LOCUS       DAFBQH010000001       100828 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.001, whole genome shotgun sequence.
ACCESSION   DAFBQH010000001 DAFBQH010000000
VERSION     DAFBQH010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 100828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 100828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.001"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(553..2166)
                     /locus_tag="LK694_000001"
     CDS             complement(553..2166)
                     /locus_tag="LK694_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK5050907.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2307..3215)
                     /locus_tag="LK694_000002"
     CDS             complement(2307..3215)
                     /locus_tag="LK694_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5050908.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3398..4522
                     /locus_tag="LK694_000003"
     CDS             3398..4522
                     /locus_tag="LK694_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK5050909.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4749..5666
                     /locus_tag="LK694_000004"
     CDS             4749..5666
                     /locus_tag="LK694_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5050910.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5659..6492
                     /locus_tag="LK694_000005"
     CDS             5659..6492
                     /locus_tag="LK694_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5050911.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6575..7693
                     /locus_tag="LK694_000006"
     CDS             6575..7693
                     /locus_tag="LK694_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBK5050912.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7687..9585
                     /locus_tag="LK694_000007"
     CDS             7687..9585
                     /locus_tag="LK694_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBK5050913.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9579..10802
                     /locus_tag="LK694_000008"
     CDS             9579..10802
                     /locus_tag="LK694_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBK5050914.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10888..12822)
                     /locus_tag="LK694_000009"
     CDS             complement(10888..12822)
                     /locus_tag="LK694_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK5050915.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13048..13704
                     /locus_tag="LK694_000010"
     CDS             13048..13704
                     /locus_tag="LK694_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBK5050916.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13778..14131)
                     /locus_tag="LK694_000011"
     CDS             complement(13778..14131)
                     /locus_tag="LK694_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBK5050917.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14323..15252
                     /locus_tag="LK694_000012"
     CDS             14323..15252
                     /locus_tag="LK694_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBK5050918.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15263..16099
                     /locus_tag="LK694_000013"
     CDS             15263..16099
                     /locus_tag="LK694_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBK5050919.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16347..17123
                     /locus_tag="LK694_000014"
     CDS             16347..17123
                     /locus_tag="LK694_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5050920.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17299..18042
                     /locus_tag="LK694_000015"
     CDS             17299..18042
                     /locus_tag="LK694_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5050921.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18035..19615
                     /locus_tag="LK694_000016"
     CDS             18035..19615
                     /locus_tag="LK694_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBK5050922.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19866..20345)
                     /locus_tag="LK694_000017"
     CDS             complement(19866..20345)
                     /locus_tag="LK694_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBK5050923.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20361..21113)
                     /locus_tag="LK694_000018"
     CDS             complement(20361..21113)
                     /locus_tag="LK694_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBK5050924.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21230..21300
                     /locus_tag="LK694_000019"
     tRNA            21230..21300
                     /locus_tag="LK694_000019"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21262..21264,aa:Gly,seq:ccc)
     gene            21661..22386
                     /locus_tag="LK694_000020"
     CDS             21661..22386
                     /locus_tag="LK694_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBK5050925.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22641..22907
                     /locus_tag="LK694_000021"
     CDS             22641..22907
                     /locus_tag="LK694_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBK5050926.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22904..23335
                     /gene="ruvX"
                     /locus_tag="LK694_000022"
     CDS             22904..23335
                     /gene="ruvX"
                     /locus_tag="LK694_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBK5050927.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23360..23665
                     /locus_tag="LK694_000023"
     CDS             23360..23665
                     /locus_tag="LK694_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBK5050928.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            24234..25070
                     /locus_tag="LK694_000024"
     CDS             24234..25070
                     /locus_tag="LK694_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBK5050929.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            25135..25566
                     /locus_tag="LK694_000025"
     CDS             25135..25566
                     /locus_tag="LK694_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5050930.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEA
                     KKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(25634..26956)
                     /locus_tag="LK694_000026"
     CDS             complement(25634..26956)
                     /locus_tag="LK694_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBK5050931.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(27110..28750)
                     /locus_tag="LK694_000027"
     CDS             complement(27110..28750)
                     /locus_tag="LK694_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBK5050932.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            29080..29550
                     /locus_tag="LK694_000028"
     CDS             29080..29550
                     /locus_tag="LK694_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBK5050933.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            30179..31282
                     /locus_tag="LK694_000029"
     CDS             30179..31282
                     /locus_tag="LK694_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBK5050934.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            31566..32246
                     /locus_tag="LK694_000030"
     CDS             31566..32246
                     /locus_tag="LK694_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBK5050935.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVS"
     gene            32336..33370
                     /locus_tag="LK694_000031"
     CDS             32336..33370
                     /locus_tag="LK694_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBK5050936.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            33384..33719
                     /locus_tag="LK694_000032"
     CDS             33384..33719
                     /locus_tag="LK694_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5050937.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            33703..36183
                     /locus_tag="LK694_000033"
     CDS             33703..36183
                     /locus_tag="LK694_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5050938.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            36239..37798
                     /locus_tag="LK694_000034"
     CDS             36239..37798
                     /locus_tag="LK694_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5050939.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            37795..39159
                     /locus_tag="LK694_000035"
     CDS             37795..39159
                     /locus_tag="LK694_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK5050940.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            39330..40400
                     /locus_tag="LK694_000036"
     CDS             39330..40400
                     /locus_tag="LK694_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5050941.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            40415..41083
                     /locus_tag="LK694_000037"
     CDS             40415..41083
                     /locus_tag="LK694_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5050942.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            41103..41681
                     /locus_tag="LK694_000038"
     CDS             41103..41681
                     /locus_tag="LK694_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK5050943.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            42084..42995
                     /locus_tag="LK694_000039"
     CDS             42084..42995
                     /locus_tag="LK694_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK5050944.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(43251..44168)
                     /locus_tag="LK694_000040"
     CDS             complement(43251..44168)
                     /locus_tag="LK694_000040"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBK5050945.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            44370..44807
                     /gene="zapA"
                     /locus_tag="LK694_000041"
     CDS             44370..44807
                     /gene="zapA"
                     /locus_tag="LK694_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBK5050946.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            44865..45413
                     /locus_tag="LK694_000042"
     CDS             44865..45413
                     /locus_tag="LK694_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HBK5050947.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            45528..47888
                     /locus_tag="LK694_000043"
     CDS             45528..47888
                     /locus_tag="LK694_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBK5050948.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            47975..48289
                     /gene="trxA"
                     /locus_tag="LK694_000044"
     CDS             47975..48289
                     /gene="trxA"
                     /locus_tag="LK694_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBK5050949.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            48471..50249
                     /gene="uvrC"
                     /locus_tag="LK694_000045"
     CDS             48471..50249
                     /gene="uvrC"
                     /locus_tag="LK694_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBK5050950.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            50394..51293
                     /locus_tag="LK694_000046"
     CDS             50394..51293
                     /locus_tag="LK694_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5050951.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            51286..52548
                     /locus_tag="LK694_000047"
     CDS             51286..52548
                     /locus_tag="LK694_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5050952.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            52711..54609
                     /locus_tag="LK694_000048"
     CDS             52711..54609
                     /locus_tag="LK694_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBK5050953.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(54908..55627)
                     /locus_tag="LK694_000049"
     CDS             complement(54908..55627)
                     /locus_tag="LK694_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBK5050954.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            55964..56296
                     /locus_tag="LK694_000050"
     CDS             55964..56296
                     /locus_tag="LK694_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5050955.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            56283..58286
                     /locus_tag="LK694_000051"
     CDS             56283..58286
                     /locus_tag="LK694_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBK5050956.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKHQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            58305..58775
                     /locus_tag="LK694_000052"
     CDS             58305..58775
                     /locus_tag="LK694_000052"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBK5050957.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            58796..59380
                     /locus_tag="LK694_000053"
     CDS             58796..59380
                     /locus_tag="LK694_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBK5050958.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            59395..60381
                     /locus_tag="LK694_000054"
     CDS             59395..60381
                     /locus_tag="LK694_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBK5050959.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            60371..60682
                     /locus_tag="LK694_000055"
     CDS             60371..60682
                     /locus_tag="LK694_000055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBK5050960.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            60704..62485
                     /locus_tag="LK694_000056"
     CDS             60704..62485
                     /locus_tag="LK694_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBK5050961.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            62478..63854
                     /locus_tag="LK694_000057"
     CDS             62478..63854
                     /locus_tag="LK694_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBK5050962.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            63861..64496
                     /locus_tag="LK694_000058"
     CDS             63861..64496
                     /locus_tag="LK694_000058"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBK5050963.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            64526..65881
                     /locus_tag="LK694_000059"
     CDS             64526..65881
                     /locus_tag="LK694_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBK5050964.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(66073..67467)
                     /locus_tag="LK694_000060"
     CDS             complement(66073..67467)
                     /locus_tag="LK694_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBK5050965.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFNSPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            67559..68530
                     /locus_tag="LK694_000061"
     CDS             67559..68530
                     /locus_tag="LK694_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBK5050966.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            68527..69309
                     /locus_tag="LK694_000062"
     CDS             68527..69309
                     /locus_tag="LK694_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBK5050967.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(69478..69849)
                     /locus_tag="LK694_000063"
     CDS             complement(69478..69849)
                     /locus_tag="LK694_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK5050968.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            69983..70426
                     /locus_tag="LK694_000064"
     CDS             69983..70426
                     /locus_tag="LK694_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBK5050969.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(70469..71278)
                     /gene="yidA"
                     /locus_tag="LK694_000065"
     CDS             complement(70469..71278)
                     /gene="yidA"
                     /locus_tag="LK694_000065"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBK5050970.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(71297..72664)
                     /locus_tag="LK694_000066"
     CDS             complement(71297..72664)
                     /locus_tag="LK694_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK5050971.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(72648..73478)
                     /locus_tag="LK694_000067"
     CDS             complement(72648..73478)
                     /locus_tag="LK694_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBK5050972.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            73641..74078
                     /locus_tag="LK694_000068"
     CDS             73641..74078
                     /locus_tag="LK694_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBK5050973.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            74154..74774
                     /locus_tag="LK694_000069"
     CDS             74154..74774
                     /locus_tag="LK694_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBK5050974.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            74963..75469
                     /locus_tag="LK694_000070"
     CDS             74963..75469
                     /locus_tag="LK694_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBK5050975.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            75486..76385
                     /locus_tag="LK694_000071"
     CDS             75486..76385
                     /locus_tag="LK694_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBK5050976.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            76472..77356
                     /locus_tag="LK694_000072"
     CDS             76472..77356
                     /locus_tag="LK694_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBK5050977.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(77548..77619)
                     /locus_tag="LK694_000073"
     tRNA            complement(77548..77619)
                     /locus_tag="LK694_000073"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(77584..77586),aa:Arg,seq:ccg)
     gene            77679..79013
                     /gene="rpoN"
                     /locus_tag="LK694_000074"
     CDS             77679..79013
                     /gene="rpoN"
                     /locus_tag="LK694_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBK5050978.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            79162..80253
                     /locus_tag="LK694_000075"
     CDS             79162..80253
                     /locus_tag="LK694_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HBK5050979.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            80294..81295
                     /gene="gap"
                     /locus_tag="LK694_000076"
     CDS             80294..81295
                     /gene="gap"
                     /locus_tag="LK694_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBK5050980.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            81405..82595
                     /locus_tag="LK694_000077"
     CDS             81405..82595
                     /locus_tag="LK694_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBK5050981.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            82699..83454
                     /locus_tag="LK694_000078"
     CDS             82699..83454
                     /locus_tag="LK694_000078"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBK5050982.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            83570..84868
                     /gene="eno"
                     /locus_tag="LK694_000079"
     CDS             83570..84868
                     /gene="eno"
                     /locus_tag="LK694_000079"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBK5050983.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            85268..86407
                     /locus_tag="LK694_000080"
     CDS             85268..86407
                     /locus_tag="LK694_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBK5050984.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(86474..87049)
                     /locus_tag="LK694_000081"
     CDS             complement(86474..87049)
                     /locus_tag="LK694_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK5050985.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(87054..88028)
                     /locus_tag="LK694_000082"
     CDS             complement(87054..88028)
                     /locus_tag="LK694_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBK5050986.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(88052..88096)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            88311..89279
                     /locus_tag="LK694_000083"
     CDS             88311..89279
                     /locus_tag="LK694_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK5050987.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            89392..89670
                     /locus_tag="LK694_000084"
     CDS             89392..89670
                     /locus_tag="LK694_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBK5050988.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            89788..90024
                     /gene="secG"
                     /locus_tag="LK694_000085"
     CDS             89788..90024
                     /gene="secG"
                     /locus_tag="LK694_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBK5050989.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            90127..90882
                     /locus_tag="LK694_000086"
     CDS             90127..90882
                     /locus_tag="LK694_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBK5050990.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            90885..93242
                     /gene="rnr"
                     /locus_tag="LK694_000087"
     CDS             90885..93242
                     /gene="rnr"
                     /locus_tag="LK694_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBK5050991.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRSTTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            93264..93728
                     /gene="smpB"
                     /locus_tag="LK694_000088"
     CDS             93264..93728
                     /gene="smpB"
                     /locus_tag="LK694_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBK5050992.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            94030..94749
                     /gene="atpB"
                     /locus_tag="LK694_000089"
     CDS             94030..94749
                     /gene="atpB"
                     /locus_tag="LK694_000089"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBK5050993.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            94802..95017
                     /gene="atpE"
                     /locus_tag="LK694_000090"
     CDS             94802..95017
                     /gene="atpE"
                     /locus_tag="LK694_000090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBK5050994.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            95108..95632
                     /gene="atpF"
                     /locus_tag="LK694_000091"
     CDS             95108..95632
                     /gene="atpF"
                     /locus_tag="LK694_000091"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBK5050995.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            95619..96161
                     /locus_tag="LK694_000092"
     CDS             95619..96161
                     /locus_tag="LK694_000092"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBK5050996.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            96184..97740
                     /locus_tag="LK694_000093"
     CDS             96184..97740
                     /locus_tag="LK694_000093"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBK5050997.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            97754..98656
                     /locus_tag="LK694_000094"
     CDS             97754..98656
                     /locus_tag="LK694_000094"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBK5050998.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            98679..100085
                     /gene="atpD"
                     /locus_tag="LK694_000095"
     CDS             98679..100085
                     /gene="atpD"
                     /locus_tag="LK694_000095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBK5050999.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            100099..100521
                     /locus_tag="LK694_000096"
     CDS             100099..100521
                     /locus_tag="LK694_000096"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBK5051000.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT        33230 a        17530 c        20935 g        29133 t
ORIGIN      
        1 gttaaataaa taggaacaaa aaaattaaat tatttaatac acacattagg gatcattcat
       61 tgaaggatta gaagacaacg aggatggcga aaaatatgat tacaatagaa aagtcgttct
      121 aaaccaatca gttcctagta tcacacaacg caagccagtc gatttggtga gattagagga
      181 gtgaaagcaa actttattat ataagaaaaa atattcgaaa gggatgctag tttaggtact
      241 agtgctgata gattaatatg atgcctcgta atattaggcg tagttactct agagacatca
      301 ctatggacta tgatttgtct cagagtcaca acatgttttt ttatgaaaag aaacagctaa
      361 aataattaaa ttgcgcaatg tttaaacttt cttaaactat aacctaataa atgtgaagaa
      421 ataatgcaca atttcttcac atttatcttc tattctatat acataccaac caacaaccct
      481 atatactttt tcatttttac tcctcaagta aaaataactc ctagaccgcg gctacccacc
      541 gcggtcgttt ttttattctt caatcaaaat cgctttgata cccaaacctg ccaagattcg
      601 ggcagaagta taagtattca atcctcttct gaaagtaatg taaacttttt tatcttttgg
      661 gatttcattg attcgatctc gtaactcatt cattggaata ttttttgttg ccgagatact
      721 gccagaaggt gctttattag gttcacgtat atctaaaaag aacgcttctt tcaaatcgtt
      781 ttcgggtata tcacttgctt tgatcgtttc tacgatattc gtcatttgat tgattgcgac
      841 ataaccagct atattcaacg ggtcacgggt agtagaatac ggtggagagt aaggcaactc
      901 caaatcaggc aaatcaaaga ctgtcaagtt tccggtgatt gctacagaaa gttctcccat
      961 ccgtttatcg acaccttttt ctccaaatgc ttgtccacct aaaatacgac cgctatctgc
     1021 atcaaaaatc agctttagat ttaatcttgt agcttctgga tagaaatagg catggtcaaa
     1081 gggagtgata aaaacagttt tataatttgt tatgcctgat gcttttaatt catgttctgt
     1141 cattcccgca taacttgctg tatgatcaaa gatttttgcg acacctgcac caatatatcc
     1201 gcgatagcgc ataggcgtac catttaaaat atctgccagc atatgtcctt gacgattggc
     1261 cgcactggaa agcatacttt ggatcggctg accagtcact acactagtcg tttcgataat
     1321 atcgccaatc gcatagatgt ccggcagatt ggtttgtaac tggtcatcaa caatgatctg
     1381 acctgtatct gataattgta tgccggttgc tttcaccact tcattcttcg gagaaacacc
     1441 aacagcaaaa attagcatat cagcagaaag gacggagcca tctgataata ctatttctcg
     1501 acctttatca cggatctcag taactcttgt ttctaaatga acggctaatc cttctttcag
     1561 caatttgtca taaactaaat tagcaatttc tggatcataa gggaaagcga cttgtggcaa
     1621 ttgatcgatt aaagtcacgt ctaagccgcg atgcttgaaa ttctcagcta attccagacc
     1681 cataacacct gcaccaagaa tgacgacatg ttgtggattt ttttcttcaa ggaacgattt
     1741 gattcgatcc gcatccgtca cactacggag gacaaagccg ttttctgctt caaaaactcc
     1801 gggaatatcc ggataatctg gtctagcacc agaagagatg atcaacttat cgtaatctgt
     1861 ttttatttct tcatttgtgg ataggtcttt aactgttaat cgcttagtag aaggatcgat
     1921 tgctgttact tcgtgtttag taaagacatc gatgttgttt tttgttttca atatttctgg
     1981 tgtacgttcg atcagcgagt cacggtcagt gatcacacct cctaaataat aaggcaaagc
     2041 acaacttgca taagaaatat tttctccgcg ttcatagata atgatttcgt gcgcttcatt
     2101 caatcgacgg aaacgagtgg caaatgaagg accgccagct acaccgccaa tgacaacaac
     2161 tttcataaaa acatctcctt ttctacgttg atcgtactat gttatggtaa aaaacagcaa
     2221 ataattgtgc cggaaaatta ttgtttattt tgaatagaca gaaaatatag agaaataatt
     2281 tgtggataaa acctaaaaat ttttttctat tttaaggata gataatactt tgaaggggat
     2341 aaccctgtat attttttaaa agctgaagaa aaatgatgga tcgaatgaaa tcctaatata
     2401 tctgcaattt ctgtataggt taattcattt tctagcacga gacgttttgc tttttccatt
     2461 tttagttctt taaaatagct aataacactt tttccattca cttttctaaa atgttttttt
     2521 aagctggctg gactgataaa aaaaatttgg gctaattgtt caagcaaaaa attttctgaa
     2581 agatgttctt ctaaatatcg tatgattgga tcaactaatt catctgaata aagaatatgc
     2641 ttttttttag gaatgacttt ctcatttgaa ggtattgtca ataaagcaag ttcctgttga
     2701 taatgccttg gtaaggaaga caattgaatt ttttgcagac aataagtaac taataaggaa
     2761 agcaaatgaa tgctcatctg ttggcatagt atgaaaaaag aattgatttc ctgatcatag
     2821 gaaatagctg gcttactatg ttttagataa tcaattttga actgttcatt tttcagaaaa
     2881 gtaatgatag atgttatttc ttgatctata gaaaaagatt gtcccgtaat atgtgctaat
     2941 aattgctcat caaaataaaa ctggataatg ataaaagttt gtgtttcctt ttgagtagaa
     3001 aagatttgga cttcttgctg tttatcaatg aataaaaggg aaggattatc tagttctata
     3061 gatttatgtg caaattcagc atataaatta gcgctttcat ttattagtag tcgaaaatag
     3121 ggagcagcag ataaatgaag atgggtatct atggaatagg ttagtgtttt tttgacaaga
     3181 ttggaggatt ggttgtttgt taacaaaaga accactttcc tttctctttt tatataaaaa
     3241 gctttctatt tataattaat gactattata acaaaaatga aaagccaaaa cgacaagtaa
     3301 aatatttttt tatataaata tttgtgttac taattccgtt aatatacata agtgaaagcg
     3361 ttttatataa gaacttgaaa agaaagaagg gatagacatg aaaaaaagat acaaattggt
     3421 gatagctttg ctaaacatta ccctattatt cagcttaagt gcatgtggaa aaactaacga
     3481 agaagtaaca gcaaacatcg cagatccaat gataaaaaaa gccactacat tagaagtata
     3541 tgtcgcaaac ggcaaactga caaactatgt tgaaaatgct gcaaaaatct ataaccgcaa
     3601 aactggagca aatattgact taaaaattgt gaatgtagct tcgggaacag caacagtaca
     3661 aatgatcacc ccaaaattgg taacacacga agatatgccg gatattatct ccatggcaga
     3721 cacttcagca gcaggtgtgc tggaaaaatt tcaagatgct ttttatagtg ctgatgaata
     3781 tggattttat gacaaatatg gcgatcagtt ttatgatcaa aaattgaata ttttgcgtca
     3841 acaaacgagt aatgaaacga ttattccttg ggctagtgat tttaccccag cactttgtta
     3901 ttatcaacca aaaaaatttg ctgaaatagg tgtagatttt gaagaaatca gttcttggga
     3961 agaatatatc caggtggcaa aagcattaaa agaaaaaact ggtagttttg gaattgcctt
     4021 gccagaatct ggggaccaag aattgtttat caacatgcta gcccagcaga atcaatcttt
     4081 gcttgatgaa gaaggaaata tccgtctgaa tactaaagaa gcgaaacatg ctgcaaagtt
     4141 aatcaaagaa atgatcgact cagatattgt acatttctac ggtgcacaag atgctgagaa
     4201 agcatttcaa gaatctgcaa tgtttgttgc tggcggctgg tatgctacga atatgtcttt
     4261 gaattttcct gatgcatctg acaaatggcg catggctcca ctgattcctt tttcaaaaga
     4321 aaatccagga aaaggtgcag tatcaggagg atcaagtttt tatgtcccta aagatgccaa
     4381 aaatccacaa gtggcacaac aatttcttac atttatgctg acagatgaag aatgcttagc
     4441 caacgcactg gagctgggcg tggctacatc taactcattg gcctatcaaa cagaagcagc
     4501 tgagaaaaag tttgactact aaggaggaca aaaatactat gaactattga ataaaatcaa
     4561 tgaaaataca tcaaatactg ttttaccagc cacttattca gatgctacgt cttatttgac
     4621 taccgctgca tataactatt ggcaaacagg tgattttgaa aattcatatc taaaagaagc
     4681 agaaaatttt gctcataagt acggcgtaaa attaactggt gattaaaaaa tattaggagg
     4741 acttaacgat gatgcaatct tcgttaccaa gcaagacaat aaagaacagc aagaaaaaag
     4801 caacagatac gtctcataaa aaaagtgctt atggattggt cacacctttt gttattttct
     4861 ttattgtgac gattttgatt cccatcatta tctcgtttgc ttttagtttt atgaaaattg
     4921 gctactcatt tgaatttgtt ggatttgaca attatctagc tatcttccga gatccattgt
     4981 ttattaaatc ttacacgaat gttttgatta tcatggcagg atcaattcca ataacgatgc
     5041 tattagctat ttactttgct gttttattga attcagcaca gttaaaagga aaaggatttt
     5101 ttagaactgt ctattatatt ccaaccatta catcaacggt ggctgtagct tccgtattca
     5161 tgactttttt caatccgact gggttattca atggattttt gaatgcgcta ggaattaacg
     5221 gtgtacagtg gttaacaaat cctttttgga tacgaatatc aatgatcatc acgatgattt
     5281 ggcttagtgt aggatataac actattttat ttttagctgg tcttcaaggg atttctaaag
     5341 agatatacga aagtgctgaa atagatggag catcgaaatt tcggcaattc tttttattaa
     5401 cgatccccat gctaaagcca atcattttaa tggccacagt tttagccact atcaatggct
     5461 tgggtaattt caatatccca aatatattct ttgggacttc caatggacca gaaaattcag
     5521 ctttggtcgt aggagtcaat ttatataaaa caagttttga aatggttgat tttggaaaag
     5581 cctctgctat tgcctggacg atggtgtttg tatctgttat attatcggct attcaattta
     5641 aattcggagg tgataaaaat gactaaacaa aagatacaaa gcagaatttt tacgtacgtc
     5701 attattgtgt ttggtgcctt aatatttttg atgccatatt tatatatgat tgtagcttca
     5761 acgcaaagta atcaagaaat tttaagtggc ggaatcaatt tcaagtttgg ggatatgcta
     5821 ttaaaaaact ggcaagccat catggcgaga tataattatc ccttgattat ttggaattcg
     5881 attgttattg ctgtaatcag tacagtttta gctacagcca gttctacaat ggcgggttat
     5941 gcattagcaa agtatcgttt tatggggaat aaactgttgt tcaatacagt tatgctatct
     6001 aggatggtgc ctttttttac cacattgatt cctttatttt acattatgag tcgtttagga
     6061 ttatcgaata cttatgcagg tgtgatcatc ccttcgattg ccagtacaac ttcagtgttt
     6121 atgatgcgac aatatagcag tcaatttcct acagcactga tggaggctgc cagaatcgat
     6181 ggtgccaatg agtgggtgat ttttacgaaa atcgctgtac cgattttatc acctacgatt
     6241 attactagtg gtttacttat ctttatggga agttggaatc aatatctatt tccattagtc
     6301 atgctttcag ataaagataa atatacggtt cccttaatta tcaaaaatat gacggtttct
     6361 agtacaggtg agccaattaa ttatggcgca attatgctaa ttttagcaac ttcagtgatc
     6421 ccaatggtct tgatttatgt gtgggcgcag tctaaattta aggagaatga cattggagct
     6481 gcaaataagt aaaaaaaata acgaatgtta accatctagt tcgttctagg agcaactcca
     6541 cattcataaa aattaaaaga atggcgggat atccatgaat gaacagacga aggaatggtc
     6601 aagaaaaatt ttgaatgtag cttctgaaaa gatagatgcg gaattagaca gaatggggaa
     6661 taaaattcca tatatttctt acaacggaaa atatacagac gtaataaagg acagaggaat
     6721 cgactggtgg accaatggtt tttggagtgg tattttatgg cagctctttc acaagacaaa
     6781 acgagaaaag ttcaaagagg cagcaataca acaagaacaa ttattagaat ctgccctgta
     6841 ccatttttca gacgttcatc atgatgtagg ttttttattc ttaccatcgg ctattgcaca
     6901 ttatcaacag tctcaaaaca aacatagtcg acaactaggg ttacatgcag ctacggtact
     6961 tgctggacgc tataatattt cagggaacta tttagtagca tggaatgacc ataaacctgg
     7021 ttggatgatt attgatagta tgatgaattt accattactg tattgggctt cgagggaaac
     7081 tggtgatccg aggttctcta aaattgctcg gcagcatgca gatacaacag ctaagtatct
     7141 agtgaggaaa gatggatcaa ccgggcatat cgcagaattt gatgcagaat cagggaaata
     7201 tattcggtta cttggcgggc aaggatacaa tgagaattct tgttggagca gaggaaatgc
     7261 atgggcttta tatggattcg cgttaagtta tcgttacaca aaaaatccgt actatctagc
     7321 tatagcagaa aagatagcag taaattttat tcagaaaatc gaaaaaactg gttatattcc
     7381 tctcattgat tttgctgtaa aagattcgaa tattgatacc agtgctggag tatgcgctgc
     7441 atgcggtctt ctggaattag caggatatgt ttcctctgta cgatcaaaaa tgtatcatga
     7501 agctgctatc aatatactcc aaaagacaga aaagcactat gcaaattgga atatggatga
     7561 agatgggatc attggaggcg gagcagaagc ataccataga gaactgactt ataatgtgcc
     7621 actgatctat tctgattatt tttttattga aggcttactt cgattagaac agtcggaatt
     7681 agctatttgg tagtaaaaag aggagaaaat acaatgaaac atccagtacc actattaagg
     7741 accagacagg attactttga cttatttaaa aagataaatc aaccactcca gccgttttat
     7801 cgagcaagtg gcgcacaaat tgattatata catcaagggg taggatatgg aaataaaatt
     7861 gcaggaatgg aaggattctc tcgggtttta tggggagcgg ggcccgcaat tgaccaatta
     7921 gatgaacgat ggttgaaatt aattctatca ggaataaaag ctggaacaga tccttcccat
     7981 cccgattatt ggggagaaat acatgaaaga gaccagcgta tggtagaaat gcccgcaatg
     8041 ttattagcgt tatatcatca tgaacaatta ttatggaaaa aattagatac aggagaaaaa
     8101 agccagatta ttcattggtt ctcgcaaatt tttgaatatg aatgtgcaga tggtaattgg
     8161 caatttttta agataattgt cggaacaatt atccaaaaat ttggttatcc agttaaagaa
     8221 tcagcactga aagaagcatt tgataatata gaaaattgtt atcttcaaaa tggctggtat
     8281 cgagacagtt caagaggtcg ccaagattat tataatcctt ttgcatttca ttattatggt
     8341 ttgatttata gcgtactaga accagaaaag cccattagcc agatatataa gaaccgagct
     8401 attgattttt ctagagacta tattcatttt tttgcggaag atggtgcaaa tgtgcctttt
     8461 ggtcgaagca tgatttatcg ttttgcagtt tcaagctttt ggtcagctat gttgtgggcc
     8521 gatcttttgc cttttaaatt aggtgaaatc aaggggttag tcaatcggaa tataagatgg
     8581 tggatgcaaa aagaaatatt tgatgaaaaa ggtatcctga atattggtta tacctaccct
     8641 caattgacat tgactgaacc ctataatagt acgttatctc cattatggct gaataagatt
     8701 tttttattgt tggcgctacc tgaagaccat gagtattgga cggctaaaga aatgcctatg
     8761 cctattcgtg aaggaactaa acttctttca gaagccaatt tgctcataca acatgataat
     8821 ggacatacat tttttttgaa tgcgggacaa ttagggccaa atttccatac attgaccaat
     8881 gaaaaatatt taaaatttgc ttattctagt caatttggtt tttcgatacc gcgagcgaat
     8941 caattgaaag cagaaatggc aatggattca atgctaggga ttcagcgagc ggacacatat
     9001 atcacgacca gttttaagaa tgagagcatc aaaacctatg gacagttcat cgttcgaaat
     9061 aatgtacgag acattgtatg taagaaagag gtacttgcct ctacatggga acctacttta
     9121 caggcaaaaa tcagaacttg gcttattcct tttgaaggct ggcagattcg cgtacacaaa
     9181 gtagaattag atacagaata tgttctatat gagacagggt ttgctattga atgttctcct
     9241 gagaaagaag gaataatcat agaagaagac aaggaaaact atagggtgag tgaagctggc
     9301 ttttcaggta ttatctgttt aagtgacgat actattaaac gaaaatccac agctattatg
     9361 ggtttgccca atacaaactt gatgacatgg gaaaacactt ttattcctgg attagagggt
     9421 acatttggaa aaggaaccca ttggctcgga acaggagtgg ttgctcatca agatcgggat
     9481 tatagtttag aagtatggaa caatcgtccg aaaattgatt ttgatgggac aaccgggcta
     9541 actatccaaa ataaaaacaa taaaaaaaga ctaaaattat gctaaaggct ggtggggaaa
     9601 tgcttgataa agtgatgttt gaagagataa tacatccaga aaaatggata aatgaatgtt
     9661 ataaagaatt aatggaaaaa gaatggaata atgagatcga gcgttgtcgc agacacacgg
     9721 agaggttaat caaacgtatc agtaacagtc agtatcaatt cccaagcgag tgtcctataa
     9781 aaggacgtta tccattaaga gacaatattg gttggacaga aggattttgg acaggaatta
     9841 attggttgtt atttgagaag acgggtgatc ctttatttca acaaacagct gaaaaagcag
     9901 atatagattt tatccacagg attgactgtc aaattatcac taatcatcac gatcttggtt
     9961 ttttgtattc tttatcaacc gtagcagatt ataaaataac gggaaatcga aaaatgaagg
    10021 aagcttcaat tgatgctgca gaaacattac ttcagcgttt ttgggagaag gcaggagtta
    10081 tacaagcttg gggagatcct agtgatcctt cacaacaagg tagaatgatc atggattgtt
    10141 gcatgaatct tccattattg tattgggcga gtgaagaaac tggaaatcaa aaatataaaa
    10201 aagctgctca ggctcatatt caaaatgcag cagattattt agtaagagaa gatggttcaa
    10261 cttatcatac attttatatg gatgtcaaag atggaaaacc aagatatgga agtacgcatc
    10321 aaggttatag tgataattcc tgttgggcta gaggccaagc atgggggatt tacggttttg
    10381 cattaagtta tcgttatttg aaagaacaaa aatattttga tttaagcaaa atattagcca
    10441 attattttat gaatcggttg ccagaagata ccatttgtta ttgggatttg atctttgatg
    10501 attctagcag agaagaaaaa gatacatcta gtatggtgat catggtttgt ggattattag
    10561 aattaataga atcaggtcta ttagacaaaa cagaaaagaa cttataccag tctgctgttt
    10621 ggaaaattat gcgacaattg aaagaaagat atgctacaac ggaagaagaa gatggctttt
    10681 taacacagtc tgtttataat aagcccaaaa ataatggggt aaatacacct tgtatttggg
    10741 gtgattacta ttatttagaa gctttagtga gaatgactag taattggtct tcttattggt
    10801 gaaaaaggta aatatggaat ttttgtttga taaaataaaa aaagaaaaac caaaacagca
    10861 tgaatggttt ctgttttggt tttttcgtta attttcatat tcttctaatt ccaagcttag
    10921 ttcttcccat tccgtcaatt ttgtttcttg atcttgtcta gcagcttcta aatcttggtt
    10981 cagctcattg agttttacgt gatcttccag gttttctggc gcgaccattt cttgttccaa
    11041 ctgatcgatc cattcatcga tccgcgccat ttcttcttca acctgagtaa tttttcgttg
    11101 aaggctacgg ataagctttt gctgttcttt gctttggtaa taatcagatt tgttcgtggg
    11161 agcaggagca gattctggct catcttgtgc caacaattcg gcgatttctt cttcttcctg
    11221 ctttttctct aaataataat catagtctcc tagatagact ttgctgccat tttcagagag
    11281 ttcgacgact tttgtcgcga tccgattgat gaaatatcgg tcatgggaaa caaaaagcag
    11341 tgtgccgtca taatcgatca atgcattttc taatacttct ttgttgtcaa tatctaaatg
    11401 gtttgttggt tcatccaaaa tcaaaaaatt ttctttgttc atcgctaact tggctaaagc
    11461 aacccgtgct ttttcaccgc cacttagcag agggattggt ttttctacat cttcgccaga
    11521 aaataagaaa gcacctaaag cacttcggat ctctttttct ggagtggtag gatgctcatc
    11581 ccacaattct gctaggatcg tcttgtttgt atgcagatca gcttgacctt gatcgtaata
    11641 gccgatggtg acatttgtcc ctagcgtttc ttttccacgg ataaaaggga tctggtgaat
    11701 gattgatttc agcaacgtcg atttcccgat accattcgga cctactagag caatcgcttc
    11761 ttgacgacgg atatctagat tgattggttc agataaggtg acattatcat atccgatagc
    11821 ggcatcttcg atttgcaaga ctacatttcc agaaggcttt tctatactga atagaaagtt
    11881 tgcttgtttt tcatcccctt gaggacgatc cagccggtcc attttttcta atgtctttct
    11941 gcggctttgc gctcgttttg tggtagaggc gcgaacaagg ttccgagcaa cgaagtcttc
    12001 taatttatgg atttctgttt gctgcttttc ataagccttc cattcacttg ctaactgttc
    12061 tgctttcaaa tctagatatt tcgaatagtt acctttgtaa tggcgcattt ttcttctgct
    12121 taattcatag acttcattga ctactttatc taaaaaataa cggtcatggg aaacaatcaa
    12181 taatgcacca gtatagcttt gcagataatt ctcaagccag gaaagtgtct cgatatctaa
    12241 atggttcgtt ggttcatcta gaatcaaaat gtcagggtgt tgcaaaagca tgcgagctaa
    12301 agctaaacgt gtgcgctgtc cgcctgataa agtatcgatt ttctgatcat aaaatgaaga
    12361 atcgaattta aacccatgca atacggaacg aatttcattt tcataaccat agccgtttcg
    12421 atctgaaaaa tcgtgttgca gccggtcgta ttcttttaaa taagaactat aagcaggtgt
    12481 gtctggcgta ccttcgctaa tgaaaatctc tagctcgcgc atccgctctt ccatttgtct
    12541 aacttgttca aaagctttca gcatctcgtt ccaaactgtt tcattggaat caagtcctgt
    12601 gtcctgagcg aggtatccga gagtcgctgt tttattttta gcgatctttc cttcatcagg
    12661 tgcttcaatt ccagcaatga tctttaataa agtcgatttt ccagcgccat ttctgccaac
    12721 taaagcgaca cgagcgcctg tactgatttc taaatgtatg ttttcaaata agacttctgc
    12781 accaaaatgg cgagcaatct gattggcttg taataatatc atggaatcct cttttctact
    12841 ttgtgaattc tcctcaaata aaaaactatt tttcaattag tagtctacca taaattccat
    12901 tatactgcga gatttgcaga aatgataaat aaatgaataa agaataaaat ttcacaatat
    12961 ctgagcaatg gaattgattt tttcctataa atactgctat gatggagaaa tcaaagatgt
    13021 aattagacaa tacgtggagg tcatttcgtg aaagatcata ttattccaaa ggcgacagct
    13081 aaaagactgc ctttgtatta tcgctattta aggattttaa ataatgctgg aaagaaaaaa
    13141 gtgtcttcaa ctgaattaag tgaagccgtt caagtagata gtgcaacgat ccgtcgagat
    13201 ttttcttatt ttggcgaatt aggaaaacgt gggtatggtt atgatgtaga agatcttatg
    13261 cacttttttg caaagacatt aaacgacgat caattgacaa atgttgcgct gatcggtgta
    13321 gggaacttgg gaagtgcttt actaaaatac aaattccatc aaagtaatag catacgtatc
    13381 agttgtgctt ttgatgtgaa tgaggaaatc gttgggcgca tcgtagacgg tatccccgtt
    13441 tatccaatgg aagatatgtc agaacaaatc aagatccaac aaatcgaagt agcaatttta
    13501 acgatacccg ctgaaagagc acaagaagta gtcaatcagt taacggaagc aggaatcaaa
    13561 ggaatcttga actttaccgc tgctcgattg aatgctccgt cagatgtatt gatccaaaat
    13621 gttgatttga cgaatgaatt gcaaaccttg atctatttct tgcattctga cgcaaatgcg
    13681 cattccgaaa atacagcaga ataaaaataa aaagcgattg aacaaatgaa ggcaaaactt
    13741 tcacttgttc aatcgctttt ttctgtttgg acaacgatta ttttgttcgg aagatttctt
    13801 cataagaaag ggaatcatct tctgctgtca gttcatctag tagataacgg atttttttaa
    13861 agtcgatgaa ccgtgcttgc cgaaagactt cttttccttg atgaaaaatc aggacaactg
    13921 gcgcagtcag cacttcaaat ttactagcta cttctggtgt ttcaaatgca tccaaatgaa
    13981 gtgccggaat atcataatgt gtgagaagtt cctctagtcg tggacgaaca gcgtgacaaa
    14041 cactgcaatt aggcatcgaa agataaatga cggcaacgga tgactgttgc agtatgtctt
    14101 gaacttcagt caagttagtg gtttgtttca tttttattct cccttttatg atagaagtga
    14161 tatgtatgag accttaattt aatgggaata acagaaaaag tcaattgaag tgctttggta
    14221 aaaaataaga gttgtgtaaa aatttacaga gcaacttgtt ttttttgaat aaaagcgtat
    14281 atttgcaaag cgaatgaact agaaataaaa aggaggaaac tcatgttttc ttttattcca
    14341 aattcggttt tgcagatgag tacgattttc ttatcgatcg ttattgaagc attacctttc
    14401 gtattattag gctgtcttat ctcaggcgcc ttacaagtat ttttgacccc agaacgtgtg
    14461 aagagatggc ttcctgaaaa tcgtatcttc tctattttta ctggttcgat cttaggattt
    14521 ttctttcctt catgtgaatg cggcatcgta ccgatcgttc atcaatttgt taaaaaaggc
    14581 gtaccagttc acacagcgtt tgcttttatg ctgactgctc cgatcatcaa tccaatcgtg
    14641 attttttcca cttttatcgc atttggcaat tcttggcaga tggcaagctg gcgggtgata
    14701 ggcagctttt ttgttgccct tgtgattggt gtctggcttg cctattttca aaaagacgct
    14761 gtattgaaag aaaaaatcac gtctaaagta gcagaaaatc accatcatca tgtccatgaa
    14821 gaagaacaaa gtgagaagaa tgaaccaaaa aatcagctga tccatcaaac tgggcatgtg
    14881 ttgacacatt caatcgacga attttttgat acaggtcgtt atttgatcat tggcggatta
    14941 atcgcagctg cgatgcagac ttatttacca actagaatta tgctaacgct cggctcaaca
    15001 aagattttag ccattatcat catgctgtta ttggccttta cgatgtctct ttgctcagaa
    15061 gcagatgcgt ttataggatc ttctttgtta agtctgttcg gcacagcacc tgtagctgcc
    15121 tttttggtct ttggcccaat ggtcgatatc aaaaatctac tgatgatgaa acgatatttt
    15181 acaggacgct ttattttatc gctgatcgga ctgattgccc ttagcgttat tggattcaca
    15241 ttatttattt aggagggaaa atatgctgcg gtttttgatt ttatctgggt atttcgaatt
    15301 gatgatgtac ttgcaagttt ctggcaaact cgatcaatat atcaatgtcc attatcgcta
    15361 cttagctatt ctttcaatga ttttatctgc tcttttggct ttagttcagc tgtatttgtg
    15421 ggtcaaaaac ggaaaagcag aagaaaaggc acacgagcac gatcatgacc atgggttgtc
    15481 aaaaccttat caacgtggaa tcgcttatgt actgctagct ttgccagtaa tcgtggggac
    15541 gttgtttcca acagttagtc tcgacactac gatcgtagaa gccaaaggat ttaatttccc
    15601 agtaagtaaa gaatcagtag gggatccgga gatgcagaca caatacttaa agccggatac
    15661 cagtatttat ttcaacaaat cagactactt agaccaaatg aatgagttga aagaaaagta
    15721 tggggataag caaactatca agatcacaga tgaaaactat ttagaagtta tggagctaat
    15781 ttataattac cctagcgaat tcattggcaa aaaaatcagc tatgaaggat ttgtttacca
    15841 aacaccgaac gatagcaata cagatatctt cttgttccga ttcggcatta tccattgtgt
    15901 ggcagattca ggagtctttg gtttgttgac gcatatgccg gaaggagtaa cggttaaaaa
    15961 tgatgaatgg tataaaatag aaggaaccat ccaatcagac tattatcaac cattcaagcg
    16021 tgaaatccca tctgtagttg ttacaaacgc tgagaaagtc tctgctccga aaaatcagta
    16081 tgtgtatcgt tctttttaaa agaacaaaat aaataaacat agctcagaca gctgacacgc
    16141 aaggacgtcg gttgtctggc tttttttgca tgatgtattc atttgtttga tatttatttt
    16201 agaagcagca tcctgaaagt atttgatatc ggtttcaaag gtgttggaaa agattgtgaa
    16261 gagaattaca atttattcct aacatgtatg ttgaaaagtt gtatgaaatg attttgatag
    16321 taaaatatcg agtggaggta ctttttatgg taacgcgtat atttattttg gatgatagtt
    16381 tggtttttga aaaaatgcta gtagaaattt tggaagaatc cagaatttta ttaagtcctt
    16441 ggaaatttga gatcgttcag ggattgaacg ttatacaatt tgttgaattc attaaaaata
    16501 atgaaatcta ttcctcagat atttttttcc tagacatcga tttgaacgcc tattatagcg
    16561 gtatcgaact aggcgaaatg cttagagaaa agaatgaaaa gtgtaaaatt gtttatctaa
    16621 cgtctcatcc gaataaagca ataactgtta ttaatcgaaa gattacgcct agtggttatt
    16681 tgataaaaga attcacttat gaaaaaaata aaaaaagcat ccaaagttac ttattgaaag
    16741 aaaaagaaaa ctatcctgtt caacatgcta cgaaaaaaag gtggacaact gtccgttatg
    16801 gtaatagcga tcactatatc cattacgatg aaatccttta tctaatgagc atgccaggtt
    16861 ataaaaataa aataggccta gtattgaaag atgaagagat tctcgtaaac ggcacaatta
    16921 aaaaatataa aaaatcactc gaatatgagt atttatgtaa agatttaaaa gcttatatca
    16981 ttaatactga aaacattaca tctattagca gagtaaacga aatagtaaca tttgcaaatg
    17041 gagaacaact agagatggga aagagaagta ttgataaact aaagaatttt atgcaagaaa
    17101 agatagaaaa tgaatcattt taaagaaaac aaagactcat atcttacctg ttagaagagt
    17161 gttttcctgt agaagagaac aacccaagta gcagcctagt cgtttctatc cttttttgtt
    17221 atgcactctt tttgacattt actccaccaa taacgaaggg tacaataaaa agtaataaca
    17281 aacaaaagga gtacagaaat gattgaattc atcaatgtaa gtaaaagtta cgggaaaaaa
    17341 caagcattaa aagacttgtc tctctcgata agacaagggg aaatttttgg atttttagga
    17401 cataatggtg ccggaaaatc aactacgatt aaaagtttag ttagtattat tgaaccatca
    17461 tcgggaacga ttttagtaga tggaatgaaa ttgacggaaa atcgattgag tatcaaacaa
    17521 aaaatcggtt atgtgcctga ttccccagat atttttttac aattgacggc tggagaatat
    17581 tgggacttga tttcagctgc ctatgaatta gacacacaaa aaaaagaaaa gcgtttagca
    17641 gaattgacag cgctttttga tatgtatagt catcaaaatg aaacaatcgc aagtttttca
    17701 catggaatgc gtcaaaaaac gattttgatc ggcacgttgc tgccagatcc agatatttgg
    17761 gtattggatg aacctttgca aggacttgat cctcaagcag catttgattt gaaagaaatg
    17821 atgaaggctc atgccgcaaa aggaaaaaca gtcatttttt ctacacatgt attggatacc
    17881 gcacaacagt tatgcgataa actggctatt ttgaagaaag gtgaattgat ttatcagggg
    17941 gcagttagtg aattattgaa tggctcacct gatgaaacgc tagaaaagat ttatttgaaa
    18001 atggctggaa ggcaagcgac aggagatgga ccgtatgtat aagcgacaat tattagaatt
    18061 aattcgagtt aatcttttat atgttaatcc acagctcacg aataaagcaa gagagaaagg
    18121 aaaaaaggga aagcgattaa ctatctatct tttgtctcaa aatctttttt cagggctgat
    18181 ttttttactg atttacggat ttgctatgtt tgctatgaac tttgttaaat tgccaggttt
    18241 ctttacttat tacgtggcac tttttggtat gttaggttta actcaaagta tatcagtcat
    18301 ttataatatc ttttttgaag gaaatgacct gcaagtattt ttgcctctgc cttttggaca
    18361 agaacaggtt ttttcagcaa agattttggt agttgctttg accatcattc cttttgtttt
    18421 tccgcttcta gtcatgttta ttttaaccgg atggcgagct gatctaggga ttttgcttac
    18481 tatcattatc tcaatccttt tgttttctct atttttgatt attctttttt gtctggctag
    18541 tttgattgtt tttggtttgg ctcgaacagc agtgtttaaa aaacatagaa aaacagtttc
    18601 tagtatcttg cttggcagct cgatgctgat cgcatttgct ggaatcatgt ggatgaatca
    18661 tcaaacgggg actattgaaa atgagttaac agatcggcat ccgatttcga tattattgcc
    18721 attctatgat gctgccagtc agccactttc tcaacaaggt ttattgggtt ggggaatact
    18781 agctatcacg gctctgggat ttttccttgc tgtaaaacag ctgatagtac caaagcttac
    18841 agagcaactg actgattcaa acagtgagtt caaaataaaa agaacccata aaaagaacca
    18901 aaatgtgcat cagcttttat ttagttacaa tgctcaatta gtaaaagaac ctaatttgat
    18961 tatgcaagtt ctttcttctt ccttgttaac tccaatcatt tttattcttg cttttgccct
    19021 aggaggcgaa ataaaattaa ctgatttaga taataagtta atcggtgttg ttttcctggt
    19081 agggatcgca ttggctttct taacagttaa tcagacttct tttatcagca atcttatttc
    19141 tttggatcaa gaaaattttc tgtttatccg ttctttgcca atctctatga accaatattt
    19201 aaaagaaaag ttcagattcg gatggatatt gcagagtttt ttaactggtg ggatcgcatt
    19261 attaagcgga ctgtcttttc agctgccttt gttttttatc cctagcttgc tatttggttc
    19321 tttattcggc tgctacttat tgtcactacg ttatttcgct agagattatc ggttgctgct
    19381 gctgaactgg accaatatta accaattatt taacagaggt tcaggaagtt tgggtttagt
    19441 cgcaactatg atgggttcat tgatcatcag tattattctt atcgtcttat acggaatgtt
    19501 ggctttgttt tatccatttt ggttagtgaa tattcctgtg atcggtttag ttctaatact
    19561 ttccgctcta tggatcgttt attatcaacg aacgttttgg aaaaaatttg aataaagaag
    19621 aggggccgtg acaaaaatca tgtcgcagcc ccttttttac agatagaggg tgttcaaaag
    19681 tcagtttctc agtattaagt caattttatg taaaaatgtg agaagctatt tttacaaaac
    19741 tttttttaat actcataatt acttcttatt gctcctgcgg ttacttgtcg caatgtttgt
    19801 gagatatttt gtcatcgctc aaggctgaac gtctctttca caaccttttc tttgttacac
    19861 ttcgtttatt taaaaagccg tttttttcgt ttcatcatga ttgcgtcacg ctttactttg
    19921 aaattacgga tgttatggtt aaagacttct gataatacaa gacctaaagc gattgctcca
    19981 gcaatcataa ttgcttggac cgcataatga gccgcttctc gataggattc agtaacgaga
    20041 tttcgaaccg cttgataagc taaaccacca gggactagag gaaccatccc gggaatattg
    20101 aaaatcgtta cgggcatttt caaacgttta gagaaaagat cgctgatgaa tgctactcct
    20161 aaagaaccaa gcaatgaacc aaaagcagca gatccgccta tttgaacaca gatccagtag
    20221 atcatccagc caaaagcacc agaaagacca caacaaatca atgaacgcct aggtacattt
    20281 gtaataacgg caaatgccgc agtcgctaaa aaactaaaag aaaactgaat aaatatcgtc
    20341 cccatacttg tgttcctttc ctaataaaaa agctggaaaa taaaagcaat cgcaaaaccg
    20401 atcatcgtag cagtcatcat cgcttctgtt ccgcgagaaa ctcctgagac ataatgtcca
    20461 gctagcaaat cacggatagc gttcgtaatt tgaactccag gaactaaagg catcacacat
    20521 ccgataatga ttagatcctg attgcttcct aagccaaaac gtacgctgta gatagcggct
    20581 atgccaatca ataaagaaga taaaaattcc gataaaaatt tgattctaaa aaatttcaaa
    20641 ctatatagat aagctgcgta tcccaatcct ccaatgacta aagtcacagg taaatcggtc
    20701 atctttccgc caaataacac cataattgtt ccactgatca ctgccgcgct aacgatttgc
    20761 aaccagacag gaaagaaaga acggtctcgg tcgattgctt gcagttcgca gtaaagttct
    20821 tctaatgtta gctctgaagc aacatattta cgtgaaagat catttacttt tactactttt
    20881 tctaaattaa tcgaacggtt ggtaatttgt tccattcgga tggtcgaggt acgatcaaga
    20941 ccaataaaga gaccggtttg agtgacatag ctgaccaaac ggaaatttcc agaagctaaa
    21001 gcaattcgac tcatcgtatc ttctacccga tacatctccg cattgctttc catcatgatt
    21061 ttcccagcga gtaaacaagt gtccagcaac agatctaatt cttttcccgt catagcatct
    21121 tctccaataa ttgattgatt agttttattt tactacaaaa cataccagaa gcgtaaagaa
    21181 taaaaaaact tgaggaagga ataaaagagg cgtatactgt ttttgtattg cgggtgtagt
    21241 ttagtggtaa aatcccagct tcccaagctg atgtcgcggg ttcgattccc gtcacccgct
    21301 taaaataggt tgtgaaaaag ctgctctgca acaagtaata agaacagcca agcaaaaaca
    21361 acttcttatg tttttaactt ggatggactt attaccgcag atgcaagctt ttgcgcaccg
    21421 tttaccaagc ggttgtgaaa tcagtgtatt gtcttgagta tgaagaaaat ttttgaataa
    21481 taataaatac cttacagata gagtggtgac tgattagaga cgaggaatcc gtctcttttt
    21541 tgtattttta agccatctta aaacaggttt tattttttgc tgtttctatt tctttctagt
    21601 atttatttat aggggaacat atgttaagat ttagggagct gttagtaaag gagtaaagaa
    21661 atgagcaaaa aaacaaccaa ggcgatcgtg gggatcgtcg ctgctgtgat tgcaggggca
    21721 ttcggtgttt ccaccacaca aaaagattca gatatctttc agcagattac aactattttt
    21781 gattccacac cacaagcttc tcaagtagcg gatgtgccag aatatgatgg aaaacatcaa
    21841 gaaatagaaa tcaatgggaa tcagccggaa tttacaaaag aggatcttag cctttccaaa
    21901 ggaacatggg aaagctattc aaatattgat aaattgaaca gagtaggaca agccaatgct
    21961 atgcttggaa aagatttgtt ccctactgaa aagagagagc ctttgtatat tgacccgaca
    22021 ggttggaaac aaaagaaatt aagtgacggg caatggttat ataatcgcag ccatttaatt
    22081 ggtttccagc tgactggtca aaataacaac attaaaaact tgatgacagg gacacgttcg
    22141 ttgaatgctc catacatgct tgctcacgaa aatgatattg cggcatatat caaagaaaca
    22201 aatcatcatg tacgctaccg tgtcactcct cattttgagg ggaatgaact ggtagccagg
    22261 ggagtgcaat tggaagcaga aagcattgaa gacaaaaaaa ttgaattcaa tgtctttatc
    22321 tacaatgttc aagatggcta cacgatcaat tatgagacag gacaagcaac aaaaacaaaa
    22381 aattagtagc agctattcga attttttatg aatattatga aaaaattgtg taaaaacata
    22441 cattcaaaga aatatagcgt atactttatg aaaagagtac ttttgataaa gctacagaaa
    22501 aaaggaaaaa aacatacaaa tagtgtttct taatttattc ggaagtttgg aatatttgtt
    22561 tgtagttttc cctttcttcg agtaaaataa ggttgtagaa ggtaagctgg aattaccttg
    22621 cgggaaagag gtgtagaatc atgggtttta cagatgaaac cgtacgtttt ggttttgacg
    22681 atagtcgcaa aaaagaagtg agcgaaacat tagcgattgt ttatcgggct ttggaagaaa
    22741 aaggctacaa tccaatcaat cagattgtcg gctacttgct ttctggagac ccggcctaca
    22801 ttcctcgtta tcgtgatgct cgaaatttga ttcgccgtca cgaacgcgat gagatcatgg
    22861 aagtaattgt caaggactac ctctcaaatc atggagtgaa tttatgagag taatgggctt
    22921 ggatgtcggt tctaagacag taggtgttgc tatcagtgac ccctttggct ggacagctca
    22981 aggagtagag atcattcgga tcaatgaagc agaagaagag tttggctttg atcggttgaa
    23041 ggaattagta gcacaatatg aagtagaccg gtttgtagtt ggattaccta aaaatatgaa
    23101 caattccatc ggaccaaggg cagaagcttc catgtcatac ggagaaaaga tcaaagaact
    23161 attctccttg ccagttgatt atcaagatga acgattaacc actgttcagg cagaacgtat
    23221 gctagtagag caggcaaata cttcaagagc caaacgaaaa aaagtcatcg ataaattagc
    23281 agctgtgatg atcttacaaa attatttaga cggctcaggt aagcaattat tttaataata
    23341 aaagaaaaga ggtaatctta tgacagaaca taaccattcc cacgaccatg aaggacacga
    23401 acacatcaca ttggtggatg accaaggaaa tgaaacatta tatgaaatcc tactaacagt
    23461 tgacggacaa gaagaatttg gacgtaacta tgttctttta tatcctgctg gtgtttcaga
    23521 agatgaagat gtagaattac aagcttatgc atacatcgaa aacgaagatg gaacagaagg
    23581 cgagttagaa caaatcgaaa cagacgcaga atgggacatg attgaagaag tattcaatac
    23641 attcatggcg gaagaagaag aatagaacaa aagaatgaat taataagata taaaaaaagg
    23701 gattgtgaca aacgtcttgt cacaatccct ttttccaaat aaacgatgtt caatcagctg
    23761 cccctcggca ttcgctcgaa aaacggcgaa atatgaagaa ctattttgcc atttgtttct
    23821 ctaataatca ggtcaaaacg ttgacttcac agtctcttcc ttaaacggtg ttctcctgcc
    23881 aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgtttt ttgaaattgt
    23941 tccttgtgac tcggagctga acggcaggct cacgacctct ttcttaaacg gtgttcaaaa
    24001 gcttgcatct gcggtaataa gttcaaccaa acgaaaatta tgaggagcta tttttgtttg
    24061 gctgttctta ttacttgatg caggacagct ttttcacaac ctcttttcgt ttagcactaa
    24121 atttccttaa aaagtagaaa agctttcgaa gcctcgtttg tttcttttac tgtataataa
    24181 aagaaaagag taacaatcgc taaaagatat aaacaagaga aaggaaatac aagatgattg
    24241 gtctgattta ttttgtggtg atcgttctgg cgaacacagt tggcgctatt tccgggatgg
    24301 gtggaggcgt gttgatcaaa ccgattcttg atcttatcgg cgcccattct gttgcaggga
    24361 tttcttttta ctcaacagtt gcggtattca cgatgtccat tgtttcgact gtgcgacaag
    24421 tatcaagcgg gaaatcactg aattggcaga ttgtcggttg ggtatctagt ggagctgttg
    24481 ttggaggagt agtcggcaac attgtattcg aagttttttt acagttattt gaaaacgaga
    24541 agcatgtgca gctgatccaa atttttttga cggtattgac cttggttttt gcgttcttct
    24601 acaccaagca tcatcagcca aaatttcatt tgacgtcatg gacttggtac cttatatgtg
    24661 ggggggttct gggcttttta gctagttttt taggtattgg tggtggacca gtcaatgttt
    24721 ctttgttgat gctgatgttt gcattgccaa taaaagaagc aacactatat tctctatcta
    24781 cgattttctt ttctcaactg gcaaaattgg taacgattgc tttaacaagt agctttatgc
    24841 gctttgattt atctatgttg ttttatgtta tccccgctgc tgttattgga ggtttgtggg
    24901 gagcaagatt tagcagggtt ttgtctccaa aaaaagtgac ctttattttt caagtaattg
    24961 tcctcgtggt tcttttggtc aattgttaca atgcctttgt catcttacaa acattaggag
    25021 aatctagact aacgcaagtc aaaatcactg aaacatattg ttctaaatga aaaaaagagt
    25081 atactatgat aaaaaaattc attttaaaaa atcagaaatt ggctggtgat ttggatgtta
    25141 cgggaaatgc gtttagaaga tgcagaagca ctacgtgaaa tcaatgcgga tcaattaggg
    25201 tatgatatac cattaaccgt tacggtgaga caaatgaaga aactgctgaa ggagccagag
    25261 aaaaattttt tcttggttta tgaagatcct acttctaaaa aagcagctgg ctatgtacaa
    25321 gcggagtcct atgaatcgat ttttagcgat ccaatgttca atattatggc acttgctgtg
    25381 tctaaatatg ctgaaaaaag aggcatcgga aaggctttga tgactggcgt agaagaagcg
    25441 aaaaaacgag gaataacgac aataaggctt aattctgcag aatacagagt agaagcacac
    25501 caattttatg aacacatcgg ttatcattcc gataaaatgc aaaaaagatt tttgaaaatc
    25561 ctgtaaatct ttatcaaaaa aatacccgat caccaagccg atacttgatg atcaggtatt
    25621 tttgtttttt tgactaagaa agtaattgca tcaagaaacc agagatgatc aaaacgatgg
    25681 caccaccaag tcgattgccc atttgagcaa aagcaataag ttccatccga ttagctgcag
    25741 ataagacggc tacatttcct gttccgccca tcgagttgtt gcataagcca gcagtaatag
    25801 ttgattcaat tggatataaa ccaaacaaac gaccgacaaa tcctgaaaca actgaaatcg
    25861 cgataacact tgtcagacat aagacaacaa attgccatgt caaagcagaa gctaatacat
    25921 tcagattcag caacgcaata ccgataccgg caagaacagc tgctgtcaag tttttgacaa
    25981 tcaagttatt gaacatgata gctgcatctt cataatactc tggtaatagg ttggcgattt
    26041 tacagatgac aacgataata atcataaatg cgtaagcatg gactttagga acaaaatagt
    26101 taccaatttg tcctaagatg aagaaagtca atgataccat caaaccaaca cctaactgtg
    26161 gaatggaagg tttcacagtt cgaggttttc catcgccaag atcgcctttt tccattaatt
    26221 taccattgcc gttgaatttt ggcattgatt gtccagcacg agcaactaat gctgcaccga
    26281 tgatagctaa gacatttgtc attgctgtcg caggaatcag gcgggaaatg atagaagaag
    26341 catccgtacc taaaacattt gcataaatcg ttgataaagg aacggctccg gctccaacac
    26401 caccagccat cgcaggtaat gagatgtata aaacggaatt tgcaaaacca tttccgataa
    26461 gcatgcccac gagtcctacg gcaaaaaagg agacgaccat tgaaataagt gcaacgggaa
    26521 taaaacgaac agaagctttc atcaatagat tacggttcat accaaggatg ctaccagtaa
    26581 tcaaggcagc aatataaaag tccaaaaagc ccatattgct gacaaagttt tctgtggatt
    26641 tcactacata ttcaggtaag atcccaaatg tagcaatcgc agcagaagcg aagatcgcaa
    26701 aaacggaacc accacccaaa taacttttga cgatagggat ttttgtcccg ataaaatgaa
    26761 ataaattacc gaaaataacc aaaactgcga ttggtccaat catattcgtc ggtaatttcc
    26821 ctaaagccat tgcaatgatc aggacaagga ccatgatcaa ataaagtggc aaacttacgc
    26881 cggtgatttt tgtggaccaa aaaccttctt ttttcttttc ctcaactgca gttgcagttg
    26941 ttgttttttg tgccattttt acagatcctt tcttattctg tatcatatga aaaaagccaa
    27001 agaaaagcga caaaagagca ggagttggct ggccgctgtt ttgccgcagt cgcttttttc
    27061 tttttagacg tcatacaaag tttctgtatg ttcttttact ataacgatct taatcttcta
    27121 aagatttata ttcaggaacc catttagcag attcaactgc ttctttgata tccgtgattt
    27181 cttcacgatt caagttttgt gcgacaactg attttgcgac agtttcagca atcgtttgag
    27241 aaaattcagt gatttttgct actggaggca agatagctgc tcctggtttt gttacatcaa
    27301 cgattccgcc taatgctcga ctagcttgag aaatgatttc agcatttact cgtgtagctg
    27361 ttgaagcaat caaaccaagt cctaaacctg ggtacatcaa agcattattt gcttgaccga
    27421 tttggtaagt tacaccatta tattcaacat cagcagcagg gatacctgtt ccaactaatg
    27481 cttttccgtc cgtccattct attaaatctt ttgcttttgc ttctgccaat tttgttggat
    27541 tagacaaagg catgatgatc ggacgtggtg tatgagcagc catttctttc acgatttctt
    27601 cagtgaatgc tccaggttgg gtagaagtac caatcatgat tgtcggatgg atcgctttta
    27661 caactgcttc taagtttgtc aattcatcag cattagtgaa atctgagcgt ttgcgagcga
    27721 atgggatttg accaggagtc aatccttctg tatcttcaaa caataaacct tgtttatcta
    27781 cttgatagaa atgttggcgt gcttcttctt caggtacacc ttgacggatc atctcatcta
    27841 aaaggatgtt tgcgataccc acaccagcag taccagcacc aaatgtcaaa attatttgat
    27901 ctttcaattg ttcaccggat atattcaatc cgcctaatac acctgctagg acaacaattc
    27961 ctgtaccttg gatatcgtcg ttgaaagtag tgatttgatc ttcgtatttt tctaagatcg
    28021 ctgcagcatt tcctcgtcca aaatcttccc aatgcaataa caattctggg aataattcgg
    28081 tagctgattg aacgaattga tcgatgaatt catagtaagt ttcgccttct acacgagcat
    28141 gacgattgcc taagtataag ggattattca ataattcttc attattagta ccagcgtcga
    28201 tcgatacagg caaaacttgt gctggattaa taccagcagc agcagtatag accatcaatt
    28261 tgccgatagc gatgtcaaca ccgttcacac cccagtcgcc catacctaag attccttctg
    28321 catcagtcac aacaatcaaa cgaatgtctc gtccatctgc cgcgttcttc aaacttgctt
    28381 tgatgttttc tggttcgtcg attgaaagaa atgcagcatc ttgcggtttc aagaaaattt
    28441 cgttgtattg ttcgattgaa tccgcaacga caggatcata aacgacaggc atgaattcaa
    28501 ctaaatgttc gcccatcaat ttataaaaca atgtccgatt cgtattgaat agattcatta
    28561 agaagatacg tttttccaaa tctgtttgtt tgcttaagta ttgaccataa gcttgaacag
    28621 attgttgttc cagtgtttgg acagtacttg gaagcatacc agtgattcca tattttgccc
    28681 gttcttcttt tgtaaaagct gttcctttat ttagaaaagg attatttaat aagtttaacc
    28741 ctgtaagcat gaatgattcc tcctaagttt taatacaaac gaagtataga agtcagatga
    28801 tgatatagtc aaaaagagca ggtatgataa attttattta tatgtaatat agccaaaaga
    28861 aatcaaaata gcagtatttg ttctttttat atcgacaatc cttgcttgaa tactatgcgt
    28921 atctcttata tgctagagaa agaaaagtag aagaataagg gatagatgga atggataaat
    28981 aggatttctg aggaaaaaag cacatgatag cgttgatagg agtcacctat gtcaggtgat
    29041 aaaaatacat aagtagaaaa ttaagaggag gaagagaaaa tgaaagaagt tcaattgatt
    29101 agaaaaagcg aactatcaga aggcggttgc aatgcatgtg gcgtagtaga agcgacaagc
    29161 tatacattaa aattagattc caatcaagca ataatctctg aactgacagt aggcggtctt
    29221 gtggacagtc tggcgcttgc tgaaggtttt actggagaag atatttatga gatgttttca
    29281 gaagtccgtc agttaaaaaa aggagaaaaa tgcatagagg ttcatcatga gagcccgaat
    29341 gtgaggttca aacgtggtga taatgagatg atcttcaaga atcatgtgtc tgatcacact
    29401 gaactatatg aaatcgtgaa tcaaatactg tctgggattt ttgaattagg accttatgag
    29461 ttcaaggaag aaaacggtaa tccaaaatta aatgaagaat ggcaagaaac gattgaaaca
    29521 caaagaaaca atccacactt atttcaatag tcttccacag tatttcgtaa aaatgttgat
    29581 gaaaaagaaa caccgtgaat cagaggtcgt gaaaaacctg ccctgcatca agtaataaga
    29641 acagccaaac aaaaatagct cctcctattt ttcgtttggt tgggcttatt accgcagatg
    29701 caagtttttg aacaccgttt aaggaagaga ctgtgaagtc aacgttctga cctgagtatt
    29761 agagagacaa atggcaaaat agttcctcat attttgccat tttttgagct aataccgagg
    29821 gtcagctgat tgaacaccgt tttatcgaga ggttgtgaaa aagtcgctta gtttcgagta
    29881 gtaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttga cttaataccg
    29941 agaaaatggc ttttgaacac cgtttattca aaaaaaggga ctttgacact acttttgtca
    30001 aagtcccatc ggttttttat ctatttttat tacaatcatt tcttccagag tagtgacatc
    30061 tggaatggct gaaatgtaaa cagattattt gcctgtatca atgtcgaaga gtaatataaa
    30121 aagtgtaaac gctatcatat tgttcatcat tcaaatttta tagagagaag gcattgagat
    30181 ggaaaaaaca tatcaagcag gtacaaatga aggaatagta gattttatca atatggaaga
    30241 tttagaaatc gcggcttcac aagtgattcc agctggtggt tatggttaca tcagcagtgg
    30301 tgctggcgac ttatttactt atcaagaaaa tgaacgggca ttcaatcatc gtttgattat
    30361 tcctcatgta ttaagagatg tagaattacc cgatacaact acacattttg acgaagaaat
    30421 gctgactgca ccaatcatca tggcaccagt tgctgctcat ggattagctc atgtgaaggc
    30481 ggagaaagct tctgcaaaag gtgtagcaga ttttgggacg atctatacag caagttctta
    30541 cgcttcttgt actttagaag agataagaga agcaggcggg gagaaagcac cgcaatggtt
    30601 tcaattttat atgagcaaag ataatggaat caatttggat attcttgaag tggccaagcg
    30661 taatggagca aaagcaatcg tattgacagc ggatgcaaca gttggcggaa accgtgaaac
    30721 agaccgcaga aacgggttta ccttcccgct gccaatgcca atcgttcagg cttatcaatc
    30781 aggagtaggc caaacgatgg atgctgtata taaatcttcc aaacaaaaac ttagtccaaa
    30841 agatgtagaa tttatcgctg ctcattctga tttgccagtc tacgtgaaag gtgttcaatc
    30901 agaagaagat gtctatcgat cattagaatc tggtgctggt ggaatttggg tatctaacca
    30961 tggtggccgt caattagatg gggggcctgc tgcgtttgat tcgttgcaat atgtggcaga
    31021 agcagtagat aaacgtgtac caatcgtgtt tgacagcggt gtacgacgtg gacaacatgt
    31081 tttcaaagca atcgcatcag gtgccgatct tgttgctatt ggcagacccg ttatttatgg
    31141 attgtcttta ggaggaagta caggagtcca tcaagtgttt gatttcttta aaacagaatt
    31201 ggaaatggtc atgcaattag ctggaacaca aacagtagaa gatatcaaaa aaataaaatt
    31261 gcgtgagaat cgttttattt aagaaataaa tgacgaaaaa aataggagtg cggcagatga
    31321 tgccgtactc ctatttttat agttcaagga tgtcactaat agaataattt gaaacgaaaa
    31381 aataaaattg aaatattttt tcatacttaa taaaaaagga agaatattca tgtaaacgat
    31441 tttattgttt ttttctcttt tatacaaaaa gggtcataat ttcaagtaag cggatgaatt
    31501 catgaggatt ttttgcatac tgtctctact atacttaggt cactaagaaa aaggagtgat
    31561 ttattatgaa aaagaacatg attgggacat tattactttc aacacttctt ctaggcggct
    31621 tggcaacacc agcttttgct gagggtcaag cgacttcaaa aggagatatt acctttactg
    31681 aaccaacaaa taccgttgag cctcttaacc ctactgatcc ttcaaaacca gtagagccag
    31741 cagatccgga aaatccagca actggccaga caggttcttt aacattggat gtagttcctg
    31801 aattgccatt tggtacacac gagattgaaa gcggaacaaa aacgtatcaa gtagacgctt
    31861 caaaaaacga tacaccttat ctccaagtat cagatcgtcg aggtgtaggt gcagatggcc
    31921 aagcacaagg gtggaacgtg acagtttcgg tttcagattt tgtaaacggt tcacaagttt
    31981 tgcaaggcgc tgaattagat tttggaacat ccactgttaa aagtacgtca gataacgaat
    32041 ccactggacc aactagtcaa actgtaactg ggttgagtaa agcaagtgca gcgacaccaa
    32101 tatttacagc agcaaaagat caaggtctgg gaacatggct ttctgtttat gatccagcaa
    32161 atatcacact gaaagtgccg aaagcagcag ctggaacatt cacggctgat ttgacttgga
    32221 acctcgtggc tggcccagta tcatagagta tgaaataaat tcaacaaact gcggtgaaaa
    32281 tactataggt gaaggatttt ttaccgcagt tgttgttgaa aggatgtggt ggacaatgaa
    32341 tcaaagtaaa cgactgtttg tttcgttttt ttctatttta agtctatttt tcatttttcc
    32401 gtcaatcagc caagcaaagg attcagcagg agactttgga atcaaaccgg tatttccaga
    32461 gaaccaaata gataaagcaa tcggttattt tgatttatta gtggcaccgg aacaaaatca
    32521 gacactagaa gtaattatca gcaattcttc agatgaagaa cgaacatttg aggtctctgt
    32581 caatccggcg gtaacgagtg atggtggaac gatcgattat agtcaaaaaa acccaacgtt
    32641 agatgagaca ttgccgtttg atgtccgtga cgttttgctc attgcgaaaa aggaaatcaa
    32701 tgtatcagca catgcagaaa caactatacc tatagaagtg aaaatcccag caaaaagctt
    32761 taagggacgt gtattagcgg gtattcatgt ttcacctaaa gaagaagctg aaacagaaaa
    32821 tgctacagaa ggagcacaaa tcaaaaatcg tattgcttat aatctcgcag tcgttttaca
    32881 agaaagtcaa gagacgatag aaccggatct gaaactttta tcaggagatt tggacgaggt
    32941 caacgcaaag ccaactgttc agcttcgttt ccaaaatcct cagcctagga ttatcagcaa
    33001 tttgatattt acgagcaaga tcttttatga aaatcagctt tatattgaaa atacgtctaa
    33061 tgaattttta gttgcaccaa attcgaattt tcacttgaat cttgatttag ctggtgataa
    33121 agcaaaagcc ggcgattacc gcgcagaaat catagcaaaa tcaggcgaca gtaatgaatg
    33181 gcgatttaca caaaacttta cgatcaaaaa agaaaaagca caaaaagtaa atgaaaattc
    33241 cgtttttgca gtacaagaac aatctttccc atggctttat gcaggattgg ttgctgcagt
    33301 tctagtgtta tggttgattt tgctgttttt gtgggtccac aagaaaaaga aaaaaggaga
    33361 aacaacatga tgaggtgatt tcgttgaaaa catcgatctt attgggaggg attattgcaa
    33421 gtttgctgtt cttcagctgg caagagccag tccaagcaga taccaaaagt tcaggtacga
    33481 ttacatttta tgatctaagt ccagttccgc atccaagtgg ttcagtcaat ggcgcatcaa
    33541 aacagcagac acagctaccg atttataaag aatctaatca acatggcgta ttcccggaga
    33601 cgaatgaaga aaagatccgt tttctttatt tgatcattgg tatagaatta tggcttcttg
    33661 cgtggttgtt gttatggaga agaaaggaaa aggaggatga acatgttgct acaaaataaa
    33721 ttttcatttt ttcgctcctt cttttttact tcgctttttg ttttaagtat atttatcttt
    33781 tcaccttcgg cagaagctgt tggagcaacg cctcctgcca tttcttatga tccatacacg
    33841 tatgaaaata tcgtacctcc aaatggtgga cgtagtgcag tcaatggcgg gaagcttgtc
    33901 caactaactg gggctaacca attgcctaaa ttgactgcta cttatttgaa cgatggttca
    33961 aatgatcctt ggaaaggtgg cacggtccgt tttcattggg aaagccatgt gaaactaaca
    34021 aatgtacttg atttggtgaa tatcactgca caaggatttg catttgtggc gatcatcaat
    34081 ttgccggaac atgtatcaag cagtgatgta ttaaaagcaa ttgactggaa caacgcctac
    34141 ttgtatattg ccggtgcccc catcaaggtt actagcggta gtttattatc agcaggaagc
    34201 cataccttgc gttttgggat ggggtcgtgg gatacaaata ctctattggc agatattatt
    34261 gctattatta cgcgaggcga attcaacgtg gacgatatcc cgcttcaatt tgatgttaca
    34321 gctaatgtcg cgaagatgac agaaaacgga gataaatatg atacgagtcc aaacaaaatt
    34381 ttgaccaagg gtaaattaca accttctatg tctaaaaagg ccgttttttc tgttgatttt
    34441 tatgattcga ataatattat ccaagatcgt atgttcaatt cgattttagc tccagctgca
    34501 ggtagatttt atcctaaatt aactggcgga aacttgaatc caggagcata tgcattttct
    34561 gcaagtgcag agataaatac gtggaatagt tacatctcaa caagtgattc aacaggaaaa
    34621 tataatacat ttgctgatac cacagaatac gtggatggaa ataaccagaa ccttccaggc
    34681 tctaccaata gtcggacatt gacgatgggg ctgactgaag gacgttttga tcagttgcca
    34741 tttagtcgct ttgatcgcgt agtcaactat tttacaggaa aaaacgttac acaaggagca
    34801 atactgactc atactccagc aagcacaagt ggattgaatc agacagccaa tatcgtgtac
    34861 agtgggaaag atgcgcaagg cggattgctt tctcctgttg ctttaaaggt attggagcag
    34921 tacaagctag acggaacggt tttaccaaca aacttaacag ccgatagtca gtggggaaag
    34981 ctcctttctt ctgcatcgta ccaagtcacg gccaattggt cagcaactga actaaaaaag
    35041 ggttcgatcc attacaggct ttattcgaaa ggaacccaaa aattagcgac aggatggaca
    35101 gatcaacttt tccaaacgat ccaatcaaat aacgggggtg taaatacagc tacttctaca
    35161 ctacctgcat tggcttcagg gcaatattat tttgattatc gcttggtgga cgatgtactt
    35221 actaacatgt atccgtcttt tgcgtacaaa tggcagtctg aacaagctgg aatcaccact
    35281 cttcctcaaa taacagtggc taattttcca tctgtgagtg cagatagtca gctaaaaaac
    35341 cttagcagac aacctgagac aggagatccc ctgtctgctc tatcaggaga tacgattcaa
    35401 gaaaatgtga cattcacgtt aacaaaatct ggggatgcga taaccgacaa aaaaatcacg
    35461 atctcacttc ctgaaaatac tacttatatc aatggttcct taaaattaaa tggtacaact
    35521 atcgcagata ccggcattca acaaggagtt tctgtcccag cagatctatt gagcaaaata
    35581 ggagatacca ttcatcttac ttacaactat caattaaata ctgtagatac ttctgtacaa
    35641 tccgtcagca tactgacaaa ggcagctgta ctttccagca atattacatt agcagatggt
    35701 gctaagcttc ctaacccagt tgtccaaaca tctgctaaaa caattcttgt ccccaaacaa
    35761 gaattaacat tagttaatgt accagatgat tttacatttg gtaacgatct ccccaaaccg
    35821 ttgaagacaa gttattatga agcaaaaggc gatttttctt ttgatgtgcg ggatactcgg
    35881 cttccaagca cgtctccttg gcaactgaca gggacattga cttctttatt taagaatgat
    35941 caaggtcaag aattaagcgg aacaaaatta tacttcaatc attctggatc aaaacaattg
    36001 attcagcaag gtcaaaacac gttgatttat gagagtgatg gaacagctaa aggagaggtt
    36061 ctcgttgact ttcccgatac cgatggttta ctcttagagg taaatagcag tacgaatgct
    36121 caacctggcg caacgtatca aggcatggtc acatgggaac tgacagctgg gccaaccagc
    36181 taagtgatta gaatttgtat ggatctgtga taactgtcac agatcctctt tttcaaggat
    36241 gagaaggaaa cttctgctgt taaaatcaga aatgcttgga ggagtggact tggaactttt
    36301 cagtttgctt gaaaaaaatg aacaacggca aatcaaactt ttccaacttt tattagagga
    36361 aaaaggagag atttccgtta ataacgtact tacccgctta tcagttgatc gggctacctt
    36421 gaaagaagat ctgttttatt tacaagctaa cttggaaaat atcaaacaat tggaaattgt
    36481 tatccaagaa gggaatattt tattaatcag acatggaaat atagcagcag tcgatgttta
    36541 ttatcattgc tacttgcgct attctacaaa atatcagata cttctttatc tgttggaaaa
    36601 tggctcattt gatcggcaga aacttgtcat ggaacttaat atcagttcgg ctactttgtc
    36661 tcgcagagtg aaggaattaa atgattcttt aaatgagttt catctgaaaa taaaaagcgg
    36721 gcggctgacc ggtccagaaa gtcaaatttg ttatttttat ttccaattat tttggtttgg
    36781 acgtccttat cttgaaaaca aaaaagaata cagtgattta tcaggaaaca cttttttaga
    36841 tctgctgcaa aaagagttta gttttactat tacagaagaa ggacgattga aatttgaaat
    36901 atggctttcc attatgaaga agcgaatgcg ctgtacgaaa aaatccaaac cagattatag
    36961 caatcttcct gttggattgt ttcaaacgac acaattttta ttggacctcc atacattttt
    37021 gacccgttac ttctttcaat atgcctttcc ttggaatcaa aatgaaacgt tggtttttta
    37081 tcttttcatg atcagtaact ttacattaga tacacataac tcgcatgtcc aggagttttt
    37141 gataagcgaa aaatacaaag acgacgtagt agctcaattg aatcgaacgt ttaaacaatc
    37201 gatagaagaa tatttctctt tttatgtttt tccagaagag atttcgcaaa agattgcgtt
    37261 gacagtgatg caaaatcacg ctcattttgc ctattttaga ggatggatga atatttttgg
    37321 ccaagaagga ttaaaacagc ggttaactga agtagaagat aaacaattcc tggttttatg
    37381 tgaaagattg gtcagacaaa catttgctat tgttgatttt actgaagtag acgaggaaaa
    37441 tgcccgcttt gaattatttg ctcgctatgc atctattttg cggacgctga tcaagcagtt
    37501 agaattacgt ctgcaaattg cttatgatct tccttatgaa aggctaatgg cggatatcgt
    37561 gaaagaaatc atccaggaac aattgctgaa tacgatcaaa tatgatttag tcgaatacga
    37621 gaaagaaaag cagtatgata tcattttgac tagccaattg aaagagtacc cgtcacagaa
    37681 gtccgctaaa gtttttgtat tcactagcaa cgaatataat tatgatttcc cctatttgaa
    37741 ccaattttta aaagaatgtt atttagaaaa attgaattta aggatgaata aaacatgaaa
    37801 atcgtgatca ttggtgcttc ccatgcaggc atcacagctg ccctaaatct aagaaaactt
    37861 caaccgaaag cagaagtttt actgatcgat gaagatcata aagatggact aggctatgtt
    37921 tcaaatggaa tcaaccttta tttaaaagga aagatacgta gtttagctga agttgcccat
    37981 aacatgcgga ctcttcaaga aagtggggca aaactgatca ccgaatggag agtaactgaa
    38041 cttgatccag acaaacatca gctcatcctc gcatccaaag aaggaaaaaa cgagaccata
    38101 acctatgata aattaatcgt agcaacaggt tcctctcctg tcaccttgta taaacaaatt
    38161 gaagctgaaa atgtctacac ctataaaaat ctagtgcaat ctaagcaagt acttgccgct
    38221 ttaaaagaag caaaagaagt agtgatcttc ggtgctggtt atattggatt ggaattagca
    38281 gatgctttga gaaataaagg atacattatt catctagttg attatatgcc gaatgtttta
    38341 tcaagatatt tcgataagga tatgatcaac agttttcaaa atcagctgca aactaagcaa
    38401 atcaattttt atccaaatga attcttgatc gattggaaaa aatcagaaga gaaagtagta
    38461 tccgtacaac ttctatctca agcaataaag gcagatatgg tcattttttc tgcccaaact
    38521 cgtcctaata cgacattgtt aaaagaaaaa gtggctttgt atgaggatga gactgtgata
    38581 gtcaatgaat atctgcagac aagtgatccg gacatctatg cgattggaga tatcgtccca
    38641 gtcagttttg acaaaaataa acggcatctc tttttacctt tagttactcg agctgtacat
    38701 atggcaagag cagtagcgct aactttgagt ggtcagccaa ccgcttacga cctcagacaa
    38761 aagataacag cagcagtcat tatagactac tttttaggaa ctgtagggct gacagaagat
    38821 gaagcgccat tcttggaaca aaataccggt tcgtgttcag gagaatttga tctatttcca
    38881 caatacgatg aagaaaacaa aacagtaaat gcaaagctga tctaccatcc ggatacatta
    38941 gaaataattg gcggacagct gataagccaa gaattcttat tatcagattt gaacctgctt
    39001 gcggatatcg tgaaacacaa aactacgatt ccacagctag cagttgagga ttttggattt
    39061 ctgtctgaac acacctctcg ttttcactac ctcaatgagt tagcttttaa agtactagct
    39121 aagagagcaa atagcaatag agtagataaa agactgtgag aaatatcacg gtcttttatt
    39181 gtgtggggat ataataaata ttaatcaatc gagaataaaa aattcatata atgaaatagc
    39241 ggaagaattt tttgagcata atattcgcca aaaatttatc aagtgataaa attgttgtat
    39301 aaaatgatta tttcaggagg aggagttaaa tgtttttagc attaaatgaa attatgcatt
    39361 caaagttacg ttatgcgtta gttgcagggg tgatgttttt gatcgcctac ctggtatttt
    39421 ttttgactgg gttagcttat ggcttagctc aagataatcg aacagcagtc gacaagtggg
    39481 aggcagattc gattgtgctt tcgaaagatg caaattcaaa tctagggatg tccatgatta
    39541 ctaagaagat agcagaagaa gtcgaaggtg ggaaagtggc gtatcttgcc cagacacctg
    39601 gagtcgtgac tagtaaagac agtacagaag aaggaaaaat caatgttagc ttcttcggta
    39661 tcgataaaaa tcaattcatc atgcccaatc ttgttgaagg aaaagctttt gataacgatg
    39721 atgaggcagt aggcgatatt agtttgaaag aagaatatgg tctagccgta ggagatacag
    39781 ttaagttatc tggtagtgac aagacgttta aactaactgg ctttacggat catgccaaat
    39841 tcaatgtctc accagttctc tatacaacca tcaatgctta ccaagagatc cgcttcgaaa
    39901 aagaagatac aagtgaaaat gcacggatta acgcaatagt tgtcagaggt aaaatcagta
    39961 atttacctga agatttagaa cagatcaaaa tttctaaatt catcaatgaa ttacctggat
    40021 ataatgcgca agtattgact tttggattta tgatcggatt tttgatcgtt attgcggcta
    40081 ttgtgatcgg gattttcatt tatgtactga cgatgcaaaa gattaatatc tttggtgtaa
    40141 tgaaagcaca agggatcaca ggaggattta ttgcgcgttc tgtagtggca caaacattca
    40201 ttttatcatt tgttggtatt cttcttggtc tattaggaac agtgggaaca tcccttgttc
    40261 tacctgatgc ggttccattc caaagcaatt ggctgttttt tggcgtcatt agcttgttga
    40321 tgctggtagt agctgtgtta ggcgcactat tttctgttcg gacaatcgta aaaatcgatc
    40381 cgttgaaggc aattggataa gaaggaggaa gagcatgaaa gcaatagaat ttaaagaagt
    40441 agaaaaaagt ttccaagatg gcgaccaagt agttaaagca ttgaaagaaa caaccatgag
    40501 tatcgataaa ggggaatttg ttgcaatcat cggtccttct ggttcaggta aaagtacttt
    40561 tttgactttg gcaggcggat tgcagacgcc tagcagtggc tctgtattta tcaatgatca
    40621 agaattcagt aatcaaaaag aaagtgaacg cgttaaacta cgttttaaag aaattggctt
    40681 tattttgcaa gcttccaatc tggttccttt tctgacagta aaaaagcagt tattattagt
    40741 agataagatc aaacatgaac atcgtcaaaa agaagcaaaa gaattatttg agcagcttgg
    40801 cgtggataag ttgatcaaca agtatccaga agaactatct ggtggagaac ggcaaagggt
    40861 cgcaatcgct cgtgcattgt acaatgatcc gtccattatt ctagcagatg aaccaactgc
    40921 tagtttggat tcagaaaagg cacgagaagt agtcgatatc ttggcaaaag aatcaaaaga
    40981 caaaaataaa gcaacgatca tggttactca cgatacacgg ctgattgaca aatgcgatag
    41041 aatatttatg atggaagatg gtgtattgaa agaaaaagaa taaccaaaaa gcaggtggaa
    41101 aaatggtcca tcgatacaac aaagatacac agcaaaaaat tttgaaagaa gcgactgact
    41161 tgtttatgtc caagggatat ctcggcactt caacaagaga gatcgcacaa aaagttggga
    41221 tcacccaacc caatctctat cattatttcg gtgataaaga aaaactatat acggcagttt
    41281 tagaaaacca cttaaaagat gttggaaaag ctttgagaga aatcgttcaa acaagcgaaa
    41341 cgggatttca agtaaccttg acaaaaatgg cgcagttttt gattgagaca catctggtcg
    41401 atttgtttct gatgcttcac gatcttgaaa gcaatctttc aaaggaaaca agagatcatc
    41461 ttttctttct atggaagaaa aattatcgtg aaccttttga agaaatcttt tcagcaaacc
    41521 aatctgtgct aagaaatggg atttcaaaag aaatcgcagc aaggcatttt ttcttagtgt
    41581 tagcacctta catcactcgg tcgaaaagac ctgtggaaga accgctaaat gtggcgcaat
    41641 tgatcgattt atatttgaat ggtgtattag gagaagcgta aagctgttat atagggagaa
    41701 aaatagttag gtgaagaagc aagatatgac gaagatcgtt atatcttgtt ttttttatat
    41761 aggtagtttt tcataaatac aaatttgtta ttctctcatg attatcaggg tatattttta
    41821 tttattatgt cggtgttcca ggaagtgttt taatttaata acgaagaaat taattgtttg
    41881 tagaatgaag gaaactattt attgggtaga tgaatcacca cgaatagtca aaaggagatt
    41941 tcaggatatg ccaaaaatga aaaaaatcat tttagcagta atgttatttt ctacggttat
    42001 atcttttaca gccccttttt tgcgtactta agctgatttt aagttttgac ccttagtctg
    42061 gatctacgag gtgagagtca agaatgaaaa aaataattgg agcttttgta tttgcaatca
    42121 ttatcattat tatctgtttt caaatagatg tttttggtca aaaaaagata gttggacaaa
    42181 cggtcagcag tacgaacagt acggttacaa agacaaagga atcatttgat gatacagaac
    42241 aagagacatt aatcaaaatc ggaaaaaatg accgtacact atatacagat gactcgctaa
    42301 ctaccccact tgcaactata aacggcggag agctggcgga gtttctttct gaaacaaaaa
    42361 atgcttatca gatcaagaca aatgatggct atactggata tttagccctc gaagatggaa
    42421 ccaaagtcac aaagaatata caaacaaaac cggaagagct atctgatgca gtgattgtat
    42481 tagatcctgg gcatggcgga aatgatactg gggcattaag caatgatgaa caaacagaag
    42541 aaaaagatat aacattaagt acagccataa aagtaaaaaa ggcgctagag gatgctggag
    42601 caacggttta tttgacccat actacagatg aattagtaca gcttggagat atatgcgact
    42661 acagtgaaga aaaaaaggca gatgtcttta tcagtttaca cgcagactct acggaatatg
    42721 cgaatgaagc aactggaatc acgacatatt attactatgg acaagaggaa acattagcac
    42781 agacaattgc tgactccttc actgatctgc ctctggattc aagaggaatc tccactggga
    42841 actatcaagt tcttcgagaa aatcttcaac cttctatttt aatcgagatg ggttatatga
    42901 ataatgatag tgatttaaag gaattagtaa cagacagtta tcagcagcag attgctgcaa
    42961 gtttgacaca agcattgacc acttattttc agtaataaat aagcccagcc aaacaaaaat
    43021 agctcctcat atttttcgtt tggccgggct tattatcgca gatgcaagct tttgaacacc
    43081 gtttaaggaa gaggttgtga gcctgccgtt taactccgcg tcacaaggag caatttcaaa
    43141 aaacagcttc tcatgtttga tgactattgt gacttgtgac cgaggagttg gcatgagaac
    43201 accgtttatt cggaacaagg gactgtgaca tgacttttgt cacagttcca ttattttttt
    43261 agcagcttca aagctttttc tgtattggca aaatgaagtt tggcatattt ttcaagcata
    43321 ggcatgccgc ctttctccaa tagttttgcc gctacttggt cagatttcgt tccagctcct
    43381 gatggaaggg tgattccagc ttcagctgat gctttgtgta actcttccaa aaagctggca
    43441 cgactgatga tagaagcagc agcaacagct aaatgatatt gttctccttt agtaacgaag
    43501 tacagttttt cttgtaattg atttttttct aaacgcgcat attttttaaa atttgcttcc
    43561 ggcgtaaact gatcgatcaa aatcgcttct ggtttttctg gctcgatttt ttttagcagc
    43621 aaatggatcg cttgattgtg caaagcgact ttcatccgaa cggcattgta ttttggttgg
    43681 atctcattgt atttttctgg agataagaca agcagtttat aggggatggt tttttttaag
    43741 atgtcagcaa gctggatgat ttggctatct tttaactctt ttgaatctct aacaccaagt
    43801 ttacgtaatt ctgcaagctg atcacgtttc acataagcag cacatactgt tagcggacca
    43861 aaatagctgc catttccgac ctcatcacta cctaaaacgg ataattgact gatatttttt
    43921 ggcaaagaag aggctgtttt tgctggcttg gcttgtttat cctgtgcctc cccccattta
    43981 tcagcttcta ttgctgcatt cgtaccttga aacatcactt ttccggatgt ataagcggta
    44041 atggtcgtcc cgtctttttt tgcagcaaag ctagtataag gaatctgttt atttaacaaa
    44101 taagagcgat agtatgcttt catttgacta agttggtcgt ttgttgcttt gataatgacg
    44161 ttgctcataa aattctcctc gtttttatac ataaaactat accgcaactc acgacttttc
    44221 gttttctaag agattgcgag acatagcatt tttttaaagt ttatcatata atagttaagt
    44281 atgtgaaaaa tatttgtgga gaacggtgaa agaaccaact tcatggtaca ctaaataaaa
    44341 ctttcatcaa ataggaggaa atgattcgta tggcgcacga aaaaacacgc tacaaagcag
    44401 tgattgccaa ccaaacatat actattatcg gacgggaaac caagcatcat atggatatcg
    44461 taacgaagct gatcaatgaa cagttagcag aattgaaaca attatcacca caaatggata
    44521 atgaacaagc agcaatcttg atggctgtca atgcgctttc ggatcaattg aagaaacaag
    44581 aaagaatctt ggagctggaa gaagaaacag cagaattaaa gaaaaaaatg atcaaattta
    44641 cagagcttga aaatcgtgtg aaacgcattg aagcaatcga gaacgaagct cgggaagtat
    44701 tgaaagaaaa tggtcaagca gatcatacca ttaaaaatca tgtggaagca caacagattt
    44761 taaacgagaa acgaaaagga caaattaaac aaaaatcttc taattaagat ccttttatca
    44821 aaataagaaa aatttaaaaa agttagaaga gaaaggaacg tagcatgctg agtttgctga
    44881 ttttattttt attgctaatt gcgttttttt ctggagcacg tcgcgggttt gctttgcaaa
    44941 tcatattcgc gattggatat gtattgtcat ttattgctgc ccagcatttt tacaaaccat
    45001 tagcaagtca tttggaatta tacattccat acccagctgt caccccaaca tcaaagttag
    45061 cattttttga tcaggtattt gcttttcacc tagatgaagc tttttatgca ggaactgctt
    45121 ttctgattat tttgctaatt ggctggctgc ttacccgatt tgtgggggtt tttgtccatg
    45181 gattgacgta tgtgccgatc ttgagacagg tagactggat tgcaggaggc atcctgtcac
    45241 taatcatggc ttacgttact atatttttga ttttgcagtt acttgccttt gttccattag
    45301 atgtggtcca aaatcaattc ggcgagcata gtttcgctcg ttttatcgtt gagagaacgc
    45361 catttcttac caacaagatg tatgagcttt gggtaacgaa tattttgaat taaggaaaat
    45421 agtgctggaa aatatttcgt tttttgtaca gagtagttta tttaagaagg ctgcgtagcg
    45481 tatgtccggc acagctcttt tttcatagtg aaagataagg tgataaaatg aataaccgaa
    45541 tccttgaaac attggaattt gaaaaagtca aacagatggt cagacaattt gtagtgactg
    45601 cacaaggaaa agaagaactg gcagaattgg taccagtgag tgagaaacaa acgatcacaa
    45661 actggcttca agaaactgag gatggattga aagtgcaacg tctgcgaggc gggatcccta
    45721 tacctaaact tgaaaatatc cgtccccata tgaaacggat cgagatcgga gcagacttga
    45781 atggagtgga gcttgcacaa gtttccagag tattgtctac gacaagcgaa ttgaatcgtt
    45841 tcattgatga tctttctgac agtgaaatcg aatttgcacg tttgtatatg tgggcagatc
    45901 agttagtcac gatccctgtt ttgagccgcc gtttaaagga agcaatcgat gaagacggac
    45961 gcgtgactga tgacgcttct cctgagttaa agagcataag acagaacatt cggcgaagtg
    46021 aacaagctgt tcgtgaacag ttagatggga tcgtccgtgg gaaaaatgca aagtatttaa
    46081 gtgatgcgat catcaccatg cgaaatgacc gctacgttat cccagtcaaa caagaatacc
    46141 gcggtgtatt tggcggtgta gtccacgatc aaagtgcttc aggacagact ttatttattg
    46201 aaccaaaaca agtggtggat ttgaataatc gtttgcgtca ataccaaatc gctgaacgaa
    46261 atgagattca acgtatttta agtgaacttt ctgctgaact tgttccccat cgtcaagaaa
    46321 tcatccacaa tgcttatgtg attggtaaaa tggatctgat gaatgcaaaa gcacgatttg
    46381 gtaaagaagt aaaagccatc gtgccgggaa tcagtgaaga caatcatgtc gtcttgaaac
    46441 aagcaagaca tccactgatc gatcaggaaa aagtagtttc taatgacatc acaatcggaa
    46501 aagactatca agccatcgtc attacaggac ctaacacagg tgggaaaacg atcacgttga
    46561 agactttagg tctgttacaa ctgatggggc aagcaggatt accgattcct gcaggtgaag
    46621 aaagtcaaat cggtattttt gaggaagtat ttgcggatat aggagacgaa cagtcgattg
    46681 agcaaagtct atcgaccttt tcttctcata tgacgaacac tgtagatatt ttatcaaaag
    46741 tcaatgaaaa aagtctggta ttatttgacg agctcggtgc aggaacagat ccgcaagaag
    46801 gtgctgctct agcaatcgca atcttagatg atcttggtaa aaagtctgct tatgtgatgg
    46861 caacaacgca ttatcctgaa ttgaaagttt atggatacaa tcgggcaaat acgattaatg
    46921 ccagcatgga gttcgatgtg gatacactaa gtccaacgta tcgtttgctg attggcgttc
    46981 ctggacgaag caatgcgttt gaaatctcta gccgattagg tcttgataca gaagttattg
    47041 atgaagcgaa gcagttgatg aatgatgaga gccaagattt aaacgaaatg atcacagatt
    47101 tagagaatcg ccggaaaatg gcagagaccg aatatctgga aatgcgtcat tttgtttctg
    47161 aggcacaaga acttcacgat gatttgaaag aagcttacag ctatttcttt gaagaacgtg
    47221 aaaaagaaat ggaaaaagca aagaaaaaag caaacgaagt cgtgtcagag gcggaagaaa
    47281 aagcagaaaa aatcattgct gatattcgta aaatgcaaca gcagattggg caaggaaatg
    47341 tgaaagagca tcagttgatc gatgcaaaga cccaattagc aaatctacat caagaagaaa
    47401 ctttgaagaa aaataaagta ttgaagaagg cgaaagaaca aaaaacgttg aaaccgggag
    47461 acgaagtgtt ggttacgact tacggccaaa gagggacgct tttacgtaaa aacgggaatc
    47521 agtggcaagt agaaatcggt atattaaaaa tgaatgtgtc cgaagacgag ctgacgcctg
    47581 ttgctcctca aaaagagccg acacaacgag ttatccatgc tgtacgatct gaatctagta
    47641 gtcacgtgcc gaatcaattg gaccttcgag gaaaacgata cgaagaagcg ctaagtgagg
    47701 tagatcaata tttggattct gccattctag ctggttatcc tcaagtgact attgtccacg
    47761 gaaaaggaac aggcgcactt cgcaagggaa ttactgatta tcttaaaaac catcggagcg
    47821 tcaaaagctt tgagtttgca ccggcaaacc aaggcggaaa tggtgcgaca attgttaaat
    47881 ttaagtaaag cttactaaag gtaagcagat ggttcgctat ccaagtatag aaatgctata
    47941 ctaatcatag attttaaaaa tggaggtctt tgatatgtca caagtaatta cagataagac
    48001 atttaatgaa gaaacagata aaggacttgt attgattgat ttttgggcaa cttggtgtgg
    48061 tccatgtcgt atgcaagcac cgattttgga tcagttggaa caagaatatg atgaagaaga
    48121 attcagaatt gctaaaatgg atgtggatga aaatccagaa actcctcaac aattcggtat
    48181 catgagcatt ccaacattaa tgttgaaaaa agatggtcaa gtagtagaaa aagctgttgg
    48241 tgttcattca aaagaacaac tacgtcaaat gatcgatcaa tatttataaa aacttacagc
    48301 aaaaataagc tgagggtggg actgaatcca gttccgtcct cggttttttt gcggttgagt
    48361 taaggttatt caaattaata ttttttgagt ataattacgt tgtcaggctg gattgttttt
    48421 ttgacagaca tgctataata actttaattg aatatgaagg ggagtaccag atgaacgaac
    48481 gaatcaaaaa caaattagct ttacttcctg atcagcccgg ctgttacttg atgaaagata
    48541 aaaatggaac gatcatttat gtcggcaaag ccaaaatatt aaaaaatcgt gtccgctcat
    48601 attttactgg cagccataat acgaaaacgg aacggcttgt tagtgaaatc gaagactttg
    48661 aatacatcgt tacagaatcg aatatcgaag cattgctctt agagatcaat ctgataaaga
    48721 aaaatgatcc taaatacaac attatgttaa aggatgataa aacctatcct ttccttaaga
    48781 taacgaatga aaaatatccc cgtttggtga ttactcgtaa agtactaaaa gacaaagcac
    48841 tgtattttgg accatatccc gatgtcggtg cggcgaatga gacaaagaag atcctcgatc
    48901 gattatttcc tctgagaaaa tgcaaaccta gtcagacgaa ggagccttgt ctttactatc
    48961 atttgggaca atgtctgtgt ccttattatt tcgacgtaga tccgaaagtc tatacgaaaa
    49021 tcgttgatga agtgaaaaag tttttaaacg gaggacacga aaaaatcgag tcagagatcc
    49081 atcagaaaat ggaaaaagct gctgaaaaca tggagtttga aaaagctgca gaataccgag
    49141 accagatccg tgccattgag acaatcatga ctcgccaaaa aatgacaaat actgatttac
    49201 tggatcgaga tgtatttggt tatgcgattg acaaaggatg gatgtgtgtc caagtgtttt
    49261 tcgtaagaca aggaaagcta attgaacgag atgtttcaat gtttcctttc tataacgaag
    49321 cagaagaaga tttcttgact tacctcggtc agttttatca ggaaaatgag catttcattc
    49381 ctaaagaagt cttgattcct gatacgatcg acaagcccag tgtagaagct ttgttagcta
    49441 cgaaagtgct gcaaccaaaa cgaggagaaa agaaaaaatt agttgaattg gcgaataaga
    49501 atgcctcagt tgctttgaat gaacggtttg atctgattgc cagaaaacag gaacggacga
    49561 tcggagcagt tgaacgttta ggaaacgcga tgaatatccc gaccccaata cgtatcgagt
    49621 cttttgataa ctctaatatc atggggacga atcctgtggc tgcgatggtc gtattcattg
    49681 atggaaaacc ggcaagaaaa gaatatcgga aatataaaat aaaaacagtc aacggaccgg
    49741 atgattatgc atcaatgaga gaagtcatct atcgcagata ttcacgagtg attcgcgaag
    49801 ggttgccttt acctgatttg atcttgatcg atggaggaaa aggacaagta gatgtggcaa
    49861 aagatgtctt agaaaatcaa ttaggaatcg atattcctat tgctggcatg gcaaaaaacg
    49921 ataaacataa aacaaatgag ttgctattcg gtatagattt ggctgtagta ccgcttgagc
    49981 gaaattcacc agagtttttc ttactccagc gaatccaaga tgaagtccat cgattcgcaa
    50041 tcactttcca tcggcagcta cgcagtaaaa acagttttgc ttcccgatta gatgaaattg
    50101 aagggttagg accaaaacga aaaaaaatgc tgcttaaaga gttcaaatcg ttgaaaaata
    50161 tcacagcagc cacgacagaa gaattacaag aaatcggact gccgaaaaat gtcgcacaaa
    50221 acgtatttga taaattacat caggaatgag gaaagggcac tttacaaaga aagtaaagtg
    50281 tcctatcatt ttatgtacaa caaaaagagc gtttacgttt tgttggtaaa taataaatcg
    50341 cttacgtctg gtgtctaagg atatatgtga tttcttgaag aaggagtgaa gaaatgttag
    50401 aagtcaagga tcttgttaaa acttttggga actacacagc tgtcgatcat gtctctttcc
    50461 aaatccccga tggaaaaatc atggggctaa ttggccaaaa cggggctggg aagacaacga
    50521 cttttcgttt gatcttggat tttttgacac aagaccaagg agaagtgtta tggaatggcc
    50581 atcatcttgg ggagaaagat tatgatgtca tcggttatct tccggaggaa cggggacttt
    50641 atccgaaagt aacgattcaa gaccaactgc tttattttgc agaacttaga gggaaatcaa
    50701 gaaaagagat tgaaccaaaa atcgattttt ggatggaaaa atttcaggta aaagggaaaa
    50761 agacggataa agtaaaatct ttgtctaaag gaaatcagca aaaagtccag ctgatcgcca
    50821 cgttaatcca tgaacctaaa ttgatcattt tggatgagcc attcagcggg ttagatccgg
    50881 taaatgctga attgctaaaa gatggaatat tggaattgaa aaaacatggt tcatgtgtca
    50941 ttttttctag ccacaatatg gataatgtag aaaaaatctg cgatcatttg atcatgttgc
    51001 gaaacggaaa aatggttttg aatggaaaag tacatgagat tcgtgaatca tttggccgta
    51061 cgaagttatt cttggaatct ggtttaagcc aacaggaagt agaagaaatc gaaggcgtga
    51121 aaaaagtgat ccttcatgaa gacaatacgt tagagattac cttagaagat cctgaagctg
    51181 ggaaagaaat ctttactcgt gcgactcagt tcggctacat tcctatgttt aaccagcaac
    51241 cgccaacttt ggaagagatt ttcaaattga aagcaggtga aatcgatgcg taagttttgg
    51301 attattacca aagatgtcta tttaaaaaat gttaagtcga tttctttctt gatcatgata
    51361 ttagtccctt ttatcatgct gggtatcatt tatgtagcag gaaactttgc tcaacaaaat
    51421 agtgatgtca ataaaatcgg cgtattggct gataatgagc aaatcagtca gcaaatgagc
    51481 caagtcaaaa cagatgattt tgagttcaaa gcagttgatt ctgaagaaaa agcacaaaaa
    51541 gagctttcgg aagaaaaaat cgatgcgtat ttagtagtct caactgagaa tgataagata
    51601 aatgggatgc tttatagcga aaattcatta ggacaaagta cacaactact gatacaacaa
    51661 caactgactg gcttgcaatc gatgcttcga gcaagtcaat taggcatttc accagaagaa
    51721 gtagctagct tgagccaaca agcaagtttg actcgacaaa aagtcaattt tgattcaaaa
    51781 ggaaaaatga cagttggaga agataactcg acagtccaat atgtcgtaag ttatgtagca
    51841 acgattgtct tgttcattat tattttgacg tatgcgcaga tcattgcaca agagatcgct
    51901 tctgaaaaag gcacgcggat catggaagtg atcctgtcca gtacgactgc acaaaagcat
    51961 ttttacggaa aactaacagg tgttttactt gttgcattaa cgcaaatggc attatacggt
    52021 attatttttg gtataggata cagtcaattc aaagatatgg aaatggtcaa aaatatatta
    52081 gacggtatct cattggataa cttgattgga ccattcttgt ggtattctct tctattcatg
    52141 atctttggca ttttgatttt ttctgtttta gcagctttat gtggctcttt ggtcaacaaa
    52201 gcagaagata cagcaaaagc aattttgcca gtgacttatc tatctctagg cggatatatg
    52261 cttggattga tactaggagc ttcagatcca aacaatatcg ttattcggat cacttcgtat
    52321 attccattct tatcttcata tattatgccg gttcgcttag caaatgagac agtcggtatg
    52381 ggtggagcat tgatttcctt gttggtactt gtagccgtaa cattcttatt gatgatcttg
    52441 tctgcaaaca tgtacaaatc gaatgtactc gtgtatagcg aaggcggatt atggagttca
    52501 ttgaaacaat cgatttcaat tatgagaaat gaacgaaaaa aagcataaaa tatagaaaaa
    52561 tatagaagga agctggacat ttattgttcc cagcttcttt tttgtataga cctatttcta
    52621 tttttttatt atattggata tatatattca ttgaaaatag agcataacaa ctttataatg
    52681 ttgagtatac caatttttgg aggcgtttta atggaccgct atttagattt actttcagag
    52741 aagttcccta caacggaaga tgtcatcacg gaaatcatca atctcgaagc aattttgcag
    52801 cttccaaaag gaacagagtt atttttgagc gatatacatg gcgaatttcc tgcttttgat
    52861 catattctgc gtatcggttc aggaaatctt aaagaaaaga tcaaagacct tttctctgaa
    52921 caaatgacgg aaacggagat cagccaattc actattttta cggcttatcc agaatatgtg
    52981 ttgacgactg attggtacaa aaaacaagat aaatcccagc tgatccatcg tttgatcgat
    53041 cttttacggt ttacgacagt caaatacact cgatcaaaag ttcgaaaagg attgccgaaa
    53101 gaatatagct atatcattga ggaactcttg tatatcgatg atcgtatcaa cgggaaaaaa
    53161 gactatgtaa aaaaaatcat tgaacaactt gtcttgatga aggaagaaga aaaattttta
    53221 acaaaacttg cgcagacgat ccaaaaatct gtcatcgatc atttgcatat cgtgggtgat
    53281 atttttgatc gaggaactca agcagccaaa gtaatggatc gaatcatgga tttttcctct
    53341 gtagatatcc agtgggggaa tcatgatatt ttgtggattg gtgcgtattg cggctctgag
    53401 gcatgtttat tgacgctttt acggattgcc gcacgctaca actatttatt tgaattagag
    53461 aaagaatatg gattaaattt acgtccatta ttttcttttg cccatcaaac ctatcagaag
    53521 aatccagtat ttacccctaa aaacaggaaa gatctatctg aaagagagca agaagaatta
    53581 gaaaagatcc atcaagcact ttctatcctc caattcaaat cagaacatca gttgttgaat
    53641 cgacgacctg aatttaaaat ggacgatcgt aaattgttgg aaaaaatcaa ctatgaggaa
    53701 tcaacgatcg ttttagaagg tcagctctat gagttgaaag ggacttgttt tcaaacgatc
    53761 cagccagatg atcctaaaaa attcttggaa gaggaacaaa aagtgatcga tagtttgatg
    53821 tattcattcc agcactcgtt aagaatgcaa aaacacatga cgtttttgat cgaaaaaggg
    53881 ggaatgtatc tcacgaacaa tcagcatgta ctgttccatg gctgtatacc tgtagatgaa
    53941 aaggggcagc tcttagcatt tgaattagac caatcttatc gcggaaaaga actgctggga
    54001 ttttttgaaa aacagataac tgaaagcgca aaagatttga ctgcaaatga agatttggcg
    54061 accgatttga tttggtatgc gtggagcggg ccattttcgc ctttattcgg caagagtaaa
    54121 atggcaactt ttgagcgcta ttttctcaca gagcctcata cgcacgtcga aaaaagcaat
    54181 ccctattatc acttgcgaga tgaagagtgg ttcagtgaaa aaattttggc agaattcgat
    54241 gcaaaagaag aaggttcagc aatcatcaat ggacacacgc cagtaaaagt aaaaaaagga
    54301 gagtcaccga tcaaagcaaa cggaaaagca tttgttattg acggaggtct atccaaagct
    54361 tatcaaaaaa caaccgggat tgctggctac tcattactat gcaattctta cggtttccaa
    54421 atcgtgacac attatccgtt cttgcctatt gaacaacaat ttgcaaaaaa atctgatcaa
    54481 acgaatgtaa aaaaagtgat cgagcagcag ctcccacgca aacttaatcg gaatacgaca
    54541 aaaggaatgg aattacagca acagattact caactccaaa ggctgttgga ttatcgaaag
    54601 gacgattgag aaaaatgcta gccaacattg ctcaatagac taaaaagcga ctgcaacaaa
    54661 aggcgtgtcg cagtctcctt cggaattagc tcaaaaaacg gtaaaatatt agaagctatt
    54721 tttctgttta tttctctaat actcaggtca gggcgctgat ttcacgacct cttccttaaa
    54781 cggtgttctc ctgccaactc ctcggtcaca agtcacaata tccaacaaac atgagaagct
    54841 gttttttgaa attgctcctt gtgactcaga gttgaacggc aggctcacga cctaatcaga
    54901 aaaacgatta aaattcattc acaaagaaat cgaataaacg ctgttcagat gaatctacac
    54961 gatgagaaag ttccggatgc cattggacac caaggatttt gtgcatatta tctttacttt
    55021 ggactgcttc aatgagtcca tcgttggaaa aggcgatggc ttttaacaaa ggagaaagat
    55081 ctttgactgc ctgatgatga taggaattca caaagtatga atcagggaga aggtcactta
    55141 ataaactgtc cgaaacgatt ttgacttcgt gagttgcaaa ctgcggggca gtgggctgtt
    55201 gttcgtgttt tacggaccat tttggatata atgaaagatc ttgataaagt gttccctcca
    55261 acacgacatt caatagttga agtcctcggc agactgcaaa aatcggtttt ctttgtttca
    55321 atgcttccaa gatcaatgcc tgttcgaatt ggtctcgttg aatatttgtt tccgtcagtt
    55381 ttggatgagg gtcttccatg taaagttgcg gactaacatc ctgaccgcct gcaagaatga
    55441 gtttgtcgat tttctcaatt agttgaggcg ctgaatctgg agacatgact ggcagaataa
    55501 gaggtaatcc ttgagcatct aaaactgcat ccacaaatcc ctgcggtgta tagcttactt
    55561 gatttccttg gaaagtatct gtcgctcgaa tcagttgatt cccggcaatg ccgatgattg
    55621 gtttcatttt tattccccct aaagtagact aattgtaaca aggatttaaa aaaatttaaa
    55681 ctatgtaaga cttgccccta tacttatctg ttcttgctgt gttactatct gtaaagagaa
    55741 ttttcgtgtg aaattcatga cgatagttga aaatattgct taatttgtta taataaaaag
    55801 taagcagaaa taaaaaggtt gggcgtaagc ttcaaggatc tagttcttca aggggtgaga
    55861 aggacatttt attgaagggg cctgcctata ctaagttatc tttagaggta catactttca
    55921 atgaaaaaag aaagcatgta cctctttttt aaatcgaaag gaagtgaagc aagtgttaga
    55981 agctttggaa caaatccgaa aagcagaaga agaaaatgag aagcgtgctc gaaccctacg
    56041 gcagcagttg aagacctatg cagaagaaaa agagcaagaa ttggagataa tccaaacgaa
    56101 cagtcggcaa cagctgaaac agttggtaga agaaaaagaa caattcgaaa agcaagaatt
    56161 agaaaaagaa aaaatgaaat tgaaagcaga agcagacaag gtgcaagcaa ctttgaaaga
    56221 ccaatatcaa aagcatcaga gtcaagcaat cggtgcaatc attgaaaggg tgaaagaaac
    56281 ttatggccgt cactaagatg gaaaaagtga ccctcatttc agataaaaga aatcaggaga
    56341 ggattttaca agcagtacaa ggaatccaaa atgttgaaat ccgggatctg tatcaaaata
    56401 gaacaaacaa ccagtgggta gaaaaatatt ttccagatac cacgattatt gataaagaag
    56461 caaaattaag cgctttaggc aatcggctga aagacatcca agaagctatc caattcatac
    56521 aacaccacgg cgatagccgt caaaagaaac tccatttgaa acatcaagaa ctttccttac
    56581 aagacttgga agcaaattat tccgaagaaa atttcatgaa taaattagaa gaagtccttg
    56641 atttaaaaga aaagtgggaa cagctaactg aaaaacgtga tcgtttagaa gaagaggaag
    56701 aatggcttct tcattggcaa cagctggata ttgctccaag tatgtatgca agtaagacga
    56761 ccatttttga gatggggact gtgagtgcag caaattttga aagtttcaag gaagaactaa
    56821 gtaagcttga tgacgtttat atggaagaaa tagattatac gccaaaacaa gtttatcttt
    56881 cgtatgttgc tttaaaaaaa tcagttgctg ctatagaaga aattgctggg aaatacgggt
    56941 tcaataaaga aacgtatcct tatgaacaaa atcctaaaga aaaattgcaa gaaaccaaac
    57001 agcaattatc cattgttcac gagcagcaaa aaaagataac gacagcgtta ggtcagtgca
    57061 gcggctatat ccaagaattt gaatgggtag aagaagtcac acttgccgtt gcagaacggg
    57121 aaaaagtaaa agaacggttt atccatgcca cttatctgat cgttttgcaa ggttgggtag
    57181 atgcagaaga aaaacagaat ttgatgcatg tgttaaacga aactgtatca gaagaagata
    57241 tatacgttag tttcgaaaca ccaaactcag aagagattca gcaggaagtc cctactaaat
    57301 tgaaaaacca tccgcttatc gaaccttttg aattgctgac tgaaatgtac agtctgccaa
    57361 aatatgaaga agtagatcca acgccttgga tgacaccatt ttatcttgtc ttcttcggga
    57421 tgatggtcgc agatgttggt tacggattgc tgatgctcat tgggacaata ctagctcaaa
    57481 aactatttgt tttaccccgc gggatgaaac gtttcgtaaa attctttgag attctctcgg
    57541 taccatcgat catctgggga ttgatctaca gttcattttt cggacaggca ttaccaaaag
    57601 aactatttgg tatccgcttg cctttcccaa tcctatctac gacggatgac gtcaatacga
    57661 ttttgatttt atccgtgatt ttcggtctga tacagatctt ggttggatta ttcgtggcag
    57721 caaaggaaaa tatcaggagg aaagattact tgagtgctgt cagtgatggt tttgcctggc
    57781 aagggatcct gattgggatc gttattgcat tgattggtgt catgttgata aaaaatacag
    57841 catttgttta tcttggcgcg gggctatcga tcattgctgc tttatgtatc ttaatcgtgc
    57901 cgattatcca atcgccgtct aaagtcaagg gtgctgccaa aggagcgtat aatctttacg
    57961 gattgactgg ttatatcggt gatttagtca gttatacacg tttgatggca ctaggtattt
    58021 caggcggaag tattggcgcg gcattcaata tgctagttgc tttcatgccg ccagccgctc
    58081 gattcagtgt agggatcctt ttgatcatcg ctttacacgc actgaatatg tttttgacac
    58141 tgttaagtgc ctatgtccat ggtgcacgat tgcagtatgt cgaatttttt ggaaaatttt
    58201 atacaggagg cggacgtgcc tttgacccgc tgaaaacggc ggaaaaatac gtcaacatca
    58261 atcataagaa aaaaaacaat aaataattgg aggattttta tacaatgatg gattacttaa
    58321 ttagtcaaaa tggtggaatg gtatttgcag tattagcgat ggcaacagcg acgatttttt
    58381 cagggattgg ttcagctaaa ggtgttggaa tgacaggtga ggcagcagca gcgttaacaa
    58441 caagtcaacc cgaaaaattc ggacaggctt tgattctgca attgcttcca ggtacgcaag
    58501 ggttgtatgg attcgttatc gcctttttga tttttatcaa cttaggaaat gatatgtcag
    58561 ttgtacaagg attgaacttt ctaggcgctt ctctgccgat tgcttttaca ggtttgtttt
    58621 caggtatcgc ccaagggaaa gttgcagcag caggtatcca aattttagcg aaaaaacctg
    58681 aacatgctac aaaaggaatc atttttgctg cgatggttga aacatatgcg attttaggtt
    58741 tcgttatttc cttcctatta gtattgaatg cctaagcata aaggaggaaa agcagatgaa
    58801 cgccattgaa aaaatcatct ctcagatgaa tgaagcagca gagcaagaaa gagcggcgtt
    58861 agagcaagaa gagcgaatga agatcgatca aaactttgaa caaaaacgta cgcaaatcga
    58921 aacagaacat caaaaacaaa aagaaaaaca aatcgaatta cttgaaaaaa aataccgtca
    58981 gcttaggaat cgccaacaag tagaagtacg acaagaaaat ttgaatgcga agcaggaatt
    59041 tttacgacga ttgtttgcag atgcaatagc tgaaatggag aattgggatg aatctgaaca
    59101 aatccagttt atcaaaaatt cattgtattc attacctttg acaggcaaag taacattcat
    59161 tgctggagaa aaatcagcag cttatctttc tcagacattg ctggatgaat ggaacaatga
    59221 acttcccttt atgatggttt taagtgatga aactgttgct gatcaagcgg gttttctgat
    59281 aaacgatcaa ggagttcagt ataattttct tttcagcagc cttgtacaag atatccaagg
    59341 aacgatgagt tttgagattg ccaatcaatt attcgaataa ggagggatca gtgtatggaa
    59401 taccacgaac taaatccctt gattcgcgga agagagcttg aacttttgtc caatgagaca
    59461 tttgaacgga tgattcaaac aaattctgtc gaagcacttg gtgaaatatt gaaatccacg
    59521 atttacagtc catatattta tgacggatat gaatatgact tcgaacagaa tcttttaaag
    59581 gagcagagcc gtctatttcg ctggttaaag gaatcagcac cggagcctga aatcgtttgg
    59641 atctatacga tgcgctatac tttccataat ctgaaggttt tgacaaaagc cgagatgaca
    59701 aatcaaaatc tggaccattt gtatatcgac gatggatttt attcattaga aaccttgaag
    59761 gatgccattc atacgcaagt ttcaaatgaa ctccctgaaa gcattatgga cagtatacga
    59821 gaagtacgtg attattttga agagtctacc attcttcagg gaattgacgt gatttatgat
    59881 cgacagtttt tgacagagca gcgacggctt gccgataagc taggatatca agaattgctg
    59941 acagaagtca ttgcttttat tgacttgacc aatatcacaa cgatggctcg aggaatattg
    60001 cagaaaagaa catatgggtt catgacgacg gtactttcca gctcgggaag tatcgaaaaa
    60061 gaagtatttt tgaactttgt agataaagat atggaagatt acacgaattt tctattagag
    60121 acagattatg ctgattttct gcgccctgtc atccgtgagg gaaatcttga tctcgtaaag
    60181 cttgaacagt tgaaagatga ttatctaagt tcattgtatc aagaagcaca aacccaggct
    60241 ttcggaccac tgccactact agcctttttg aacgcaaaag aggtagaaag caaaaacctt
    60301 cgtttactgg ttatcggcaa aagaagccat ttcgaaccag aaacaatcag aaagcgggtg
    60361 agacaagtct atgacgcata agatcggtgt agttggcgac aaagattctg ttttgccatt
    60421 caaattgttt ggctttgatg ttcgttatgg tacaaccaag caaaccgttc gtcaggcaat
    60481 tgaagaaatg gcaaagaatg agtatggcgt gatttatgtc acagagcaat gtgcagcatt
    60541 gagcccagaa acgatcgaac gttacaagga acaaatgata cctgctatcg tattgattcc
    60601 caatcatcaa ggatcattag ggatagggct ggaagaaatt caaaaaaacg tagaaaaagc
    60661 agtaggtcaa aatatcttat agtatgaaaa ggagatagaa attttgcaaa ttggaaaaat
    60721 cataaaagtc tccggacctc ttgtaatggc tgagaacatg tctgatgcca gtatccaaga
    60781 catgtgtcta gtgggagatc taggagttat cggagaaatt atcgagatgc gtggcgatgt
    60841 ggcgtctatt caagtatatg aagagacttc tggaattggt ccaggcgaac cggttcgctc
    60901 tactggtgaa gctttatctg ttgaattagg accagggatc atttcacaaa tgtttgacgg
    60961 aatccaacgg ccattagata cctttatgga aatcacacaa agtaatttct tagggcgcgg
    61021 agttcaattg cccgcattgg accatgagaa aaaatggtgg ttcgaaccaa cagtagaagc
    61081 cggagaaacg gtgagtgctg gagatgttat cgggatcgta gaagaaacaa aagttataaa
    61141 acataaaatc atggtgccaa atgggataaa aggaacgatc aaaacaatcg aaaaaggttc
    61201 attcactatt gatgaaacag tatgtgtcat tgaaaccgaa aatggtgaca aaaatttgac
    61261 gatgcttcaa aaatggccag taagacgtag tcggccaatc aaagaaaaat taaacccgga
    61321 tgcacccatg atcactggac agcgagtgat tgatacattc ttcccagtga caaaaggagg
    61381 agcagcagct gtacctggtc cttttggtgc agggaaaaca gtcgttcagc atcaaattgc
    61441 aaaatgggcc gatgtggaca tggttgtcta tgtgggatgt ggcgagcgtg gaaatgagat
    61501 gacggatgtt ttaaatgaat tccctgaatt gattgaccct aatacaggag aatcgttgat
    61561 ggaacgtacg gtattgattg ctaatacatc aaacatgcca gtggcagctc gagaagcttc
    61621 tatctatacg ggaatcacaa ttgcggaata tttccgtgac atgggttatg acgttgctat
    61681 catggcagac tccacttcac gatgggcaga agcacttcgt gagatgagcg gacgtttgga
    61741 agaaatgcct ggtgatgaag gttatccagc ttatcttggt tcacggcttg cggaatacta
    61801 tgaacgatct ggaagagtca ttgcattagg atcagaaggt cgcgaaggaa gtataaccgc
    61861 aatcagtgct gtatccccgt ctggagggga tatttcggaa ccagtcacac aaaatacatt
    61921 gcgggtcgta aaagtattct ggggtcttga ttccagttta gcccaaaaaa gacattttcc
    61981 ttcgatcgat tggatacaaa gctattctct ttattcaaca gaagtaggga agtatatgga
    62041 tcatatcctt caagctgact gggcatccat ggtcaccgag ggcatgcgta ttttgcagga
    62101 agaacagcag ctgaatgaaa tcgtgcgttt ggtagggatt gattcccttt cagataatga
    62161 ccgtctgaca ttagaagtgg caaaatccat tcgtgaagat tatttgcagc aaaatgcttt
    62221 tgatgatgtc gatacattca cttcaagaga gaaacaattc aaaatgttga agatgatctt
    62281 gacctttggt gaagaaggac gccgtgcgtt gtcgttaggt gcttatttaa gtgagatcat
    62341 ggacggtacc gtatcgattc gtgaaaggat cggacgaagc aaatatatcc cggaaacaga
    62401 attagaaaaa ctagatcaaa ttattggtga aatcaagcaa acgatgcaaa cgatcgtttc
    62461 agaaggaggc atgagcaatg attaaagaat atcgtacgat cggtgaagtc gtcggtccat
    62521 tgatggcggt tgaaaaagtt gctggtgtca aatatgaaga attaatcgaa gtccgtatgc
    62581 aaaatggcga gattcgtcgt ggacaagtat tggaagtcca agaagataaa gcaatggtcc
    62641 aaatattcga aggaacaagt ggtatcaatt tacgtgactc ttctgtacga tttttaggcc
    62701 atcctttaga actaggtgta tcggaagaca tgatcggtcg agtattcgat ggtttgggac
    62761 gaccgaaaga taacggacca gagatcctgc ctgaaaagca tttggatatt aacggagaag
    62821 taatcaatcc agtagctcgg gattatccag atgagtttat ccaaacaggt atctctgcaa
    62881 tcgatcattt gaatacatta gttcgtgggc aaaaattacc agtattttct ggctctggtc
    62941 ttcctcataa agaattagct gctcagatcg cgcgacaagc aacggtactc gattcatcag
    63001 atgatttcgc cgtagtcttt gctgctattg gaatcacttt tgaagaagct gaatttttca
    63061 tggaagattt tcgtcaaacg ggcgcaatcg accgttctgt catgtttatg aatttagcaa
    63121 acgaccctgc aatcgaacgt atcgctactc ctcgtatggc tttgactgct gcagaatatc
    63181 ttgcttatga aaagggaatg catgttttgg ttatcatgac agacatgacc aactatgcag
    63241 aggcattgcg agagatttct gcagcacggc gagaagttcc aggaagaaga ggatatcctg
    63301 ggtatcttta cacgaactta gctactttat tcgaacgagc aggaagaatc cgcggactaa
    63361 aaggatcggt tactcaaatt ccgattctaa caatgccaga agacgataaa actcatccaa
    63421 tccctgactt gactggttat attacagaag gacagatcat cttgactaga gagctttaca
    63481 agagtgggat tcagccacca attgatgtac tcccgtctct ttcgcggtta aaagataagg
    63541 gaactggacc cggaaagaca agagaagacc atgcagctac gatgaatcag ctttttgcag
    63601 cctatgcaca aggaaaacag gcaaaagaat tagccgtagt tttaggtgaa tctgctttat
    63661 ctgatgtaga caagatctat gcgaagtttg ctgagcggtt tgaaaaagaa tatgtcaatc
    63721 aaggattcta cacgaaccgt tcgattactg aaacacttga tctagggtgg gaactgctat
    63781 cgatgttgcc aagaaccgaa ttaaaacgaa tcaaagacga catgctggac aaatatctgc
    63841 cagaagggaa gtgagttctg atggcacgat tgaatgtgaa tccgacccgc atggaattga
    63901 ctcgattgaa aaaacagttg acaactgcca caagagggca taaattatta aaagataagc
    63961 aagatgaact gatgcgtcaa ttcatcctgt tgattcgaaa gaacaaccag ctgcgtcaag
    64021 aaatggaaga gtctatgaca gaagcaatgt ctgattttgt attggcaaat gcttcgatca
    64081 cggaaccttt catcgaagaa ttatttgttt tgccggcgga aaacgttgat ttgtctatcg
    64141 tagacaaaaa tatcatgagt gtaaaagtac ctgtgatgaa ttttgaatat gatgaacagc
    64201 tgacagacac acctttggaa tacgggtatt tgaattccag tgccgaactg gatcggtcaa
    64261 tcgatcggtt tactaccctg cttccaaaac ttttggaact gacagaaatc gaaaaaactt
    64321 gtcaactgat ggcgagtgaa atcgaaaaga cacgtcggcg agtcaatgca ttagaatata
    64381 tgacgatccc tcaattagaa gaaacgatct attatattcg tatgaagctg gaagaaaacg
    64441 agcgcgcaga agtaacaagg ttgatcaaag taaaaaatat gggtaccact gactaagtgg
    64501 tatctttttt taaaagagga aaacgatgat taaacaaagc aaacaaataa agaaacgttt
    64561 gccggctgtt cagcttctcg cattaggatt ttttctcctg attttagttg gcggcggcct
    64621 acttacccta ccatttttta gtaatagtgg gcatgggaca aattttatcg atgcgctttt
    64681 cactgcaact tctgctgtct gtgtaacggg attgacaaca ttgaatacag cagagcattg
    64741 gaacagtgct ggtcaatttt tgatcatgct tttgattgag atcggcggtc ttggttttat
    64801 gatggttccg attctatgct ttgcattgat aaagaagaaa gtcagtctca gcacacgtat
    64861 tatattaaaa gaagcgctga atctagaaga agtatcaggt gtgatgaagc tcatgcttta
    64921 catcctaaaa ttggcctttg ttatccaatt gattggtacg gttgctttat cttttgtatt
    64981 tgtgcctgaa tttggctggt tgaaaggtat ctggtacagt ttgttccatg caatctctag
    65041 tttttgtaat gcagggtttg atttgttagg agacagttta gccaatcacc agcaaaatgt
    65101 ttatttgatc atggtggtat ctgctttgat catttctggt gggttaggct tcatcgtatg
    65161 gcgtgattta ctggaatatc atcagaaaaa gaagatgacg attcattcaa aaatcgcatt
    65221 aaccgttaca ggtacccttt tattaggcgg attcgttgtg ttctttttca cagagcgcaa
    65281 tggggcaacg ctagtggaag ggaatatgtt tcaacgattg gcaaacacct tttttatgag
    65341 tgtgacacct agaacggctg gatattattc agttgattac ctccatatga gccatgcagg
    65401 tctgatttta acgatgtttt tgatgtacat cggcggaaca tctgggtcga cggccggcgg
    65461 attgaaaaca actacgttag gtgtgttgct gatccagatg cgttcgatgt tgaaaggacg
    65521 tacgagagcg gaagcatttg gccgtaccat ccgacaaggc gcagtgttgc gggcacttac
    65581 tttatttttt gttacgttga cgttgtgtgt agtcgccatc atgattttat cggtgactga
    65641 aacgatacct aaaacttctg gcatcgaata tattgctttt gaggtcttct ctgcgtttgg
    65701 tacagtaggt cttacgatgg gattgacgcc agacttgact gcaataggga aattgatcat
    65761 tatagcgctt atgtacattg gacgagttgg tgtattgact gtggtcttct ctctgatgac
    65821 aaaagcacaa agaacacaat caaaatacaa atatccagaa gaaagtgtca tgattggata
    65881 atttcatctt ctgacagaaa cctagtgatt tagtaaaata agaaaataaa ataatttttc
    65941 ttgcttcttg gatactttaa gtgcaaaatg ttcgtttatt ttttgaggca ctacccgttg
    66001 ttgaacctcg ggtagtgcct cttttttatt gaaaggaatc aataagattt gattccttta
    66061 tttatcaata ggttataatt catatttttt tattttttct tttacgatac ttagtccaga
    66121 agctaaaatt tcaaggttat tagaggatag ggaaattcga acgaatgcat ctggttgcgc
    66181 acgtgtagta aagcgtctgg aatgataaag tcggataccg ttagcaagaa agtcattctc
    66241 aattatctta gggtcaatct caggatcaac tggaagaatt cgatagtaag ggacacgatt
    66301 cgttggtttt tctactttga aaagagaatc aaaaagctga ttggctcttt ttgtaagact
    66361 taatttagtt tctaagatcc tatttgcttc atttgaatgg aggatttgag tgatgacttc
    66421 cacatctaat ccagaagttt tcacatttat attaaaaatt gcttgttcta tcttgctttg
    66481 gacatgtact ggaactacaa gataagcaac tcgcaatcca gaacaaataa attttgtcat
    66541 tccagctaag tagattgttt ggttaggtaa taaattttgg aaaggaggaa tgatcgtatg
    66601 ttgatagtaa gttgttaaaa atgaatggat atcatcttca ataacccata gaccttcttt
    66661 ttcaatcact tcggctaatt gctctcgtct tttgtgagag atttgaaacc ctataggatt
    66721 attacaagaa ggcataagaa agacaccatg aatcttcttt tttctacatt cctgaatcaa
    66781 tatatcaggt cgcattcctt gctcgtcaaa atcaattgga acgatctcta aatgatataa
    66841 ctgagccaat tcaatgaaat tagcgtatgt atatcggtct gttgctattc tatcacctgg
    66901 agagaaaagt gcagaaagtg tgatagccaa tccattttga acaccggaaa caatagatag
    66961 agattctaag gacggaatga ctccttgagt ttttaaccaa tttgctgcag tagtcaattg
    67021 aaaatttgtg cctttgggat cttggtaatt aagcaaacca acaagatttg ggttattact
    67081 agttgattga ataaacggga caacgaattt attacattct tcaaaagaac taaccagacc
    67141 aaaatcaatg atatgttcgg ttacttcatt ggtagagatg gtgatggact caaagacatt
    67201 gggagaatta aatgtgccac ttccaatatt cgtatacagc aaacctttct caattccata
    67261 tttataagcc ttgccaacgg tagtaaaatt taaatctaaa taatcagcta gctctctttg
    67321 ggagggtaac tgagtgtttt tctgtaattt attctttaaa atatcttgtt ctaattgttg
    67381 aatcaacgat cgataataag gacgcttcaa tttttgtttg tctggtcgcc aagttaacgg
    67441 gtaattgtca taagaattaa taggcatttt ctccactcct taaaattgta taccatacaa
    67501 ttttaccatt gattgtattg attttagaag ttaatctttt gtgaggaggg aattgtctat
    67561 gaaaattatt aattttgagg aagtagtagc ttcttttagt tttgaagaag cgattcaagt
    67621 aatgaaaaaa tgtttttttg attatgaaag tggtaaaatc agccaaaatc ctcgattggt
    67681 ggaaatttta cctgacggaa aaggcaaaaa tatgtttgcg atgatgcctg cttatttggg
    67741 ggagaacaga tattttggca caaaagtcat taccgctttt ccggataatc atcagtatca
    67801 tttaccttct catttaggag agattttctt gtttgatgct caaaacggac tacccaaagc
    67861 attgataaac gcaaatgcag tcacctggat tcgaacagct gctgtatctg cgctggcaac
    67921 agattactta gcaaatccgc atgcagaaac acttgcttta attggagctg gtcaacaagc
    67981 attgtcgcat ttgactgcga tttctaccat tcgtcccatc actacagtgt ttgtttacga
    68041 tattttagaa gaaagacgaa atacttttat cgctaaagct aaaaaacagt atcctaaaat
    68101 aacgtttata aatgcaatat cagtagaaga tgcagtacgt ccagcggaaa tcatttgtac
    68161 gttgacctct agtaaagagg catttcttca aaaagagtgg gtaagggaag acgcacatat
    68221 caatgctgtg ggcacattta ctcctgatac aagagagata agtacatcgt tgatggaaac
    68281 ttctcaaatc tatgtagatg attactcagc tgccttaaca gaaagcgggg acctgcttat
    68341 agccatggca gaaggagatt tagaggaatc ttgtattgtt ggtacattag gcgaattagt
    68401 ttctgagaaa aaaacatttt ataaaggaga atcgaggccg acgattttcg acgcagttgg
    68461 tctagctatt gaagatcttt gttgtgcaga atatatttat aacaaattag agaaaggggc
    68521 tttaaaatga acttagatta ttacgtagtt gatgcatttg cagatgaggt gttcaaagga
    68581 aatccagcag cggtttatgt gctagaagaa tggctgccag aaggtacgat gcaaaaaatt
    68641 gccattgaaa acaatttatc tgaaacagca tttacagtga agaaaaacca agaatttgag
    68701 cttagatggt ttacgccaga tcgagaaatc gatctttgtg gtcacgcgac attagcaaca
    68761 gcatttgttt tatttaacta ttacaaaata cctgatgaaa cgattaaatt ttctactcaa
    68821 agcggtaatc tatttgtgac aagaattaaa gattattatt acatggattt tcctagtatt
    68881 atgccgaaaa aagtgccgat attgtctgaa tatgaagaag cgattggggc taagattaaa
    68941 gaagcctact tagcacgtga tttatttttc gtattagaag atgaaaaaac ggtggctaaa
    69001 ttgcagccag attttacagc tatcaaaaat tttgaattag gaataggagt catcgtgaca
    69061 gcagaaagtg tgcaaaaaga ctttgtttct agaaccttct ttccaaaatt aacaatcaat
    69121 gaagatccag tatgtggatc agcacattct aatcttattc cttattgggc agaaagaatg
    69181 aataaaacaa aattgtcagc ttatcaaatg tcatcaaggg gcggggatct agaatgtgaa
    69241 ttaaaagaag atcgtgtgat aataggagga aaagcagttt tgttttctaa aggtacctcg
    69301 tttatttgat ttctttcaaa tgaattaaaa ataaattggt ggagagtggc aaccttatag
    69361 ctacaatggt tgttaaaaaa agacgcaaac tttttcaatt taatgaaaag tttgcgtctc
    69421 attttaatta ttggtgtttg ttttttattc aatcaagagt aaaatgcccc atactaacta
    69481 tcctcggatg acttcgattt tataaccatc agggtcaatg acaaagtagt aagaaggcgc
    69541 agtgccaggt aatcctttta agtcagtaat ctcaagaccg gcagctttat gttgctcgtg
    69601 taatttttct agatcatcag ctgctatagc gatatgacca tagccgtctc ctagatcata
    69661 cgcaccatga tcatagttgt aagtcaactc taattcatag tcatccccag gaagtgtcaa
    69721 ataaacaagt gtaaatttgt tttctggaaa atcacggcgg cggctttctt caaaaccaaa
    69781 tgccttttga taaaattcga ttgatgcttc aagatctttc actcgaacac atgtgtgagc
    69841 cattttcata gattcatctc tccttttcct tttactcaat cataacaaaa agaagtaagg
    69901 aaatagttga tttgtgcttg ttcatgagaa atttctcatg taaaatggat agaaaataaa
    69961 actgttaagg tggaaactat tcatggataa accaattttt ggtgaaaaaa agcaatttgc
    70021 tgattatcaa acccgttatg cagcatatat tgtcatagaa agagatcagc agattgcagt
    70081 aatcgaggct ccaaatggtg ctttttttct tcctggcggg gaaattgaag gagaagagac
    70141 gaaagaagaa gcgattgtcc gtgagctgat agaagaaatg ggaattgtgg cagaaatttc
    70201 ttcttatctg ggagaagctg atgagtattt ttactcgaat tatcgtgcaa cttattatta
    70261 taatccagga tatttctttg ttgctgattc ttggaagcgt ataggagaac caactgaaaa
    70321 gaccaataaa atttggtggg tgagccctca agaagcgctt gtgaaattga aacgtggtag
    70381 ccaccgatgg gcagtcgaga gatggatcga aaaaaataaa aactgaaaga aagcgagtag
    70441 atcagctttc tctcagtttt tttatttatt acagtacata ttttttgatt gcttcagcta
    70501 caccgtgctc gtcgtttgat gaagtgatga catttgctgc ttctttgaca agagaaacgg
    70561 catttcccat tgctactcct agaccagcat attcaatcat ggataagtcg ttctcgttat
    70621 ctccgatagc catgatttca tcttgatcaa taccaaggtg ctgtgcgaga ttcgcaactg
    70681 ccgcaccttt acttgcttct ttatttagta cttcgaagta atagggcgca cttttgaccg
    70741 ttgtgtattt atcacggaaa gtttgcggta aacgcgcaat tgctgcatct aaaatttctg
    70801 gttcgtcgat catcatcatt ttgacgatgt tcatgtctgg tgtcatttct tcaggtgttc
    70861 gatattttag cggcatcttg actaaacttg cttcgtggat cgtataagcg ctgatatctc
    70921 gattaggcgt gtagatcgct ttgtcatcga tcgaatggag atgactgccg acacggcgtg
    70981 ccatcacttc gatctctagg aaatcttcat gtgtcagtcc gtgacgggcg atgattttac
    71041 ctgatttcgt ttgttgtacg agtgccccgt tataagtaat aacgtaatca ttatcttgaa
    71101 aaaggttcaa ctcgtttaac tgttcctgga cacctggtaa gggacggcca gtgcataaaa
    71161 cgatcgaaac accttttgca acagcagcgg caatcgcttc ttttacttct tttgttactt
    71221 ctcttttctc atttaataaa gtaccatcga tgtcgatagc gactagtttg atagacataa
    71281 tttcctccaa tatattttaa tttatttcaa tcaatgcgcc attacggata tggctggcaa
    71341 attcttgata agtttcgtca aataaatcat aatgatcacg caaggacgga tcaaccattt
    71401 cttttggaaa gaaaaatcgc tcatctcctt gttcttgtcc cgctaatgca gcaacaagct
    71461 gactaacctg tgataattct atcaaagttc cgtcatttcg caaaatctcg atttgtgtgc
    71521 gatgtctgcc ttgttctggg cgataaaaat cataaggtag atcgtagcta gaattcacag
    71581 cagtgtaata aacaggatta tatccgacct tttctactaa caaagtgagt tcttggacta
    71641 attttgaatc ccggttgcca gaaaaagtag cagatttcag tggtttacga ttcaaaaaac
    71701 gtttagctaa atcatttaaa ataggatcag cagattcaga ccattgagta aagtaagtgc
    71761 ccaagacccc atcatcaagt ttgaggtatt cttctaaagt aaagcttccg tcaagaaatg
    71821 gcagaagtaa ttgagaatgg agttcaaaac tgctggcatc ttcttgatac aactcgtgtg
    71881 cgcgatgaag cagatgctcc aaaattactt ccataccacg ggaaacagga tggaagtaca
    71941 cttgcacata catttgatat cggctaacga tataatcttc cactgcatgc atgccactca
    72001 tggaaaaagc gatcccgcct tcataaggac gaatcacacg taatattcgg gtaagatcaa
    72061 atgttccata ttctgttcca gtgaaataag catcccgaag caaatagtcc attcgatctg
    72121 catcgatctg actagaaatc atttggacaa cttgtgggtt ggggtaagtt ttttggatca
    72181 cactagctac cttttcagga aaaccttctt ctactcggtt aagaatctga tacacttctg
    72241 tttctggaga agtgatgatc tctacagtga tggcttcatg attcgtatgg aagatatgtt
    72301 caaatgtatg agagtaagga ccatgaccga catcatgcaa cagtgccgca cataaagtca
    72361 ccagccgttc ctgatcatcc cagcctccgt tgcctatttt ttcttttgaa aaatttcgag
    72421 aaaagatatc gcagattcgt cttgaaatct cataaacgcc taatgaatga gtaaaacggc
    72481 tatgttctgc tccgtgaaaa gtgaaagaag aagtgcctaa ttgtttaata cgtcgtagac
    72541 gttggacttc tttcgaattg atcaaatcta agattacttg atgctggaca tggatatagt
    72601 tatggactgg atcacgaaaa actttttcca taggcagcat ttgatattta taaggggttg
    72661 tcataatgta gctcctttat gttattacgt tgttccattc tctggatttg gttgttccat
    72721 tgctccagct ggatttcttt ttttagccag tcggtagaat cctgtaaatc acttgcagaa
    72781 tacagctttt caaaagcttt tgcaaatgct tctttgactt cttttacaaa aaggggttgg
    72841 tcaagcagtt cttcaagtgt tgccattgta gcaggatcca ctgcaggata gccatttgta
    72901 ccaaagtctt cttttaaagc agattgataa aatcgcttca tgacttgccc gcgtttttcc
    72961 tgattaccgt tgacacttaa gtacatcata acagaaatcc catttttgat ccgacgctga
    73021 gcgataccgg caaatttttt gccatggatg cttaaatcat aagttccagg acaataagaa
    73081 tgggtgattt cgaatgcttg tatcggtgcc tttggaaaag cgtatttagt caataggagc
    73141 atttgctcgt aagcgtcatc tatggttatt ttttttgtgt cactttgggg gaaaatcaaa
    73201 gaaatattca aaattccttc atctgatacg atccctaatc cacctgaatt tctgatcact
    73261 ggagcgtaac ctgcatcttt cagtgtttgg atcccgtcac ttagaaaagg agttcgaaca
    73321 tccttcatac ctaagatgac tgtgcagtca tattgccaga aatgtaaaaa gatttgctgg
    73381 tgttctttag aatattccgt taatatatct gttaatgcaa aaggggaaaa gtcgtttttt
    73441 tgataaatgt gctgatctag tcgaatcgca gggttcattc aaccggctcc tttgtataaa
    73501 ttcatatttt ctacaatatt atactctaaa atcaaagagt tgttttggaa aaaaggtttt
    73561 ctgtaaaaat ggacaagttg gttgacaaaa caaaagaaac taggaacaat gtagctatga
    73621 gaagtaaagg agaatgaata atggaattat cacaaggaac accgatttca atcaaattgc
    73681 gtacaaaagt aaaacagcaa ggggaagtgc aagattttta ttttgatttg aaagggcaaa
    73741 tggtgacaat tggcgatacg ctgtacatac gttataaaga gattcaggag gagacgggcg
    73801 atgagattcc tgtaacgatc aaattagttc cagatggtca tgtacagctg atccgtgcag
    73861 gagaaatgcg gatgcgtttg aaatttggct ataaagaacg gttagaaaca tcctatcgaa
    73921 caccatacgg tatgatccag attgctactt ttacgaaaga actgcatgtt agtttgaaag
    73981 accgtcccac aggagggaag gttcgaatcg attatgatct gttcatggga ccggaaaaaa
    74041 tcggcgaata ttatttgacc ttggatttta cagcgtaaat ataaaagaaa agtttgattt
    74101 tttgtgtctg attttgtatg ataaggagta gactgtgaaa ggacgtgtcc tgattggaaa
    74161 ttagtgtatt tgaaggtgca aacaaaagcg aattatcaat gatcgaagta gcacacgcga
    74221 tcttagaaca acgtggcgat gtcatggatt tttctgattt agctaaccag atccaaaact
    74281 atcttgggaa atcagacagt gaaattcgtg attcactcgc acaattttat actgacttga
    74341 atattgacgg cagctttatc tcattagggg ataatcgttg gggcttgcgt tcatggtatg
    74401 ctatcgattc gatcgatgaa gaagtaaacc atggaatgga cgaagaggat gaagatactc
    74461 cacgtcgccg taaacgcaag aaagtcaacg cctttatcaa tgatgatgaa gacgcgatcg
    74521 attacaatga cgatgaccca gaagatacag atctgacaga agaagatgac gatgatctgt
    74581 ttgatgacga tgatgatgaa gatgaagaaa tcgctgctta taattctgac ttgcaagaaa
    74641 tcggagcaga tgacccgaca gatgatgaag aagacttgcc aggaaatatc gaagaagatt
    74701 taagtatcat agacgaagac gatgatgatg aagattatga agaggaagaa ttttctgact
    74761 ctgaaaaaga ataaacatta ttttcttacg agaggttgtg acagagacga tcagcctatc
    74821 agaataagga ggtgtgacac gatttttgtc acaccttctt ttttgtatat aaaaatccca
    74881 agtaaataca taaaaaataa aaaaattgaa acttttagcg caccttgtac gtcatattaa
    74941 gtgaggcaga aaggagtgga gaatgatttt tactggatat aagaaaaaac ggcagatcga
    75001 aaaataccgt cacctgctga atggactcat cgaaactgat tatcaaaaaa tgttccgtat
    75061 cacgatgaat tacgtacatg ataaagaaga ggccttagat gtgatgcaag atagttttca
    75121 taaagccctg aactattttg aaaaagaaaa agagatcgag cattttagtg catggtttta
    75181 tcggatattg atccgcacgg cattagatag ttggcgcaga caaaaaagga cgcctgtgga
    75241 cttatttgaa attcaagata tcccccaaca gttaccttta gaaacttccg ttgcagagct
    75301 ccatatgatt ttagataaaa tagacagtcc ggaaaaagag attctgatcc tctacttttt
    75361 tgaaggattt cgtttgaaag aaattgcgac tatcttggat atgaatgaaa acacagttaa
    75421 aacgaaaatg taccgctcat tgaatgcttt acgtcaaatc ctagaatgaa ggagagaaga
    75481 gtgccatgta ttcccaaaaa gaaaatttaa aaaaactaaa gaaaacttat aaagaacaag
    75541 caattcccaa agggcttaaa gaggagatcc gccagcgttt tattatcgaa gagcaatcat
    75601 ttataaaagc aaagcggaga aagagacgtc tccaagggtt cggagtatct ttggctatcg
    75661 taatgattac aacaagtagc cttcttttta atagtcaagt tcgaagcttt gcagaagatt
    75721 tacccatctt aggatcagtc attgaattga tcttaggcga acgctttact gatcgatctg
    75781 aaaaaatcga tattcaggta ccgaagatca atacacaaaa tgagaaagaa aataaaacaa
    75841 ttcatggatt aaatcaaaaa tactttcgcg aaggacaagc agattttgaa aaagctgaaa
    75901 aagaatatgg aaattttgaa acggatcatt accaagtttt gggagattat cagaaaatcg
    75961 tagacgataa ccggtttctc gtgattgaaa ggcagatcac ccaaacagca gcagatagcc
    76021 atgttgaaaa aagatacgac acaatcgaca agaaaaacag tgtccagctc tcacttccac
    76081 ttttattcaa agatgacacc tatctatctg ttttgacaaa agaagtcaaa cggcagatgg
    76141 ccgaacaagt caaggaagat ccatctaagt actattggac tgagcaagat attcaagaag
    76201 ggacgataga aaaaccaaca cttgttacgc ctacacgaag tttttatttg aacaaagagc
    76261 atcaattagt tttgactttt tctcaatacg aaattgctcc tggttatatg ggtacaccag
    76321 aatttgtcat tcctaagtcc gtgacaaaaa cgattttggc atcggaagat tatttggatc
    76381 attagacgag aagtagttgc gactagcagt ttcaattttc aagtaagtac aagtaaataa
    76441 taagaagaga aaaagagaaa gaagagatat agtgaaaaga aattatttag ttatcagtat
    76501 tattgcttta tccgtgcttt tgtttttcga aattacttcg gtggcctcct tgatcaccga
    76561 agtaaaagga caaacacact cttcggagca ttcgtcacaa tctttcaaac aatcagaaca
    76621 atcaaccatt aaaagccaat caactgaaac agctaacgtc aaagaagcca ttcaagaatc
    76681 agcggaaaag gtacaaagtc agtcatcaca atcaacacaa gacagtacaa aaacgagccg
    76741 agatccggct gtcaaaagcg tcgcaatcag ctttgatgat ggaccaggag cgaccacaac
    76801 accgcagctt ctgcgaatct taaaagaaaa aaacgtgcat gccacttttt tcgtcttagg
    76861 agaaaatacg gcgcagcatc ctgaaatcgt gaagcagaca gctgaagcag ggcacgagat
    76921 tggcaatcac acatacgatc atcagaatct ggccactctt tctgcccagt cgatgacgga
    76981 ggaagtgacg aaagcagaca ctgagatcaa aaaagtcact ggcaaaaccc ccacttttgt
    77041 tcgtccgccc tatggttctg tcacgaatgt aggagcaacg atcatccagc gaccgatcat
    77101 cgaatggtct gttgattcag aagattggaa gacaagaaat cctgatttga ttttgcagaa
    77161 gatccaagca acagtatacg atggggcaat tattttattc catgatatct atcctgaaac
    77221 gattcgtgcc gttcctcaag tgatcgatta tctgaaagag caagggtatc gcatcacgac
    77281 agttggggat ttattagggc atccaacggc tgtcgagaac tattatggca gaaacgatca
    77341 tcggccagtt cagtaagtga gacaagtatc tttaataaat aacacatttt aataagtagt
    77401 tgaacacata actaccttac ttgcaaaggc ttaacaatat ctatacaggt acaacaaaaa
    77461 actgtccacc aattttcttt ggcggacagt tttcacttaa aaataaagaa gcaactattc
    77521 gtaaattatg ctaaaatagc agtagaacgc gtcctgaggg aatcgaaccc ccgtctcaag
    77581 aaccggaatc ttacgtgata tccactacac taaggacgca agcactggga taattgtaac
    77641 taaacagata aaaaattaca aggggattgg acaaaaaaat gaaatttgag caaaattttt
    77701 cacaaaaaca gcagcagaca cagaaactag ccatgacgca acagttgcaa cagtccatcc
    77761 aagttttaca atataacagt gaggagctgt tggcattcgt tgagaatcaa gcaatggaaa
    77821 atccattggt ggaagttgtt gagccggaat ggcagccaga ttacataaaa gcttcttcat
    77881 cttcttatga aggagaagag acaaattacc taaatcagat tcctgataca aaagggtctt
    77941 tatttgattc attgatcgaa caagtccatt tgaattaccg agatactttt ttgaggaaaa
    78001 tcgttcttta tcttgtagaa tacatcgatt tgaacggatt tttgacgatt agtttagaag
    78061 aagccggaaa agagattgga gctacgccta tccaaatgct cgatgcgttg actctgattc
    78121 agcaattaga accagctgga gtaggtgcac gtagtttgca agaatgtctc atgcttcaaa
    78181 cagaacgaga tgattatgcg ccggaattag cttatatcgt gttggaagaa tgttttgagg
    78241 aactggtgga gcgaaaatgg aaagaaatcg cacaacgttt ttctgttgat ctacatgctg
    78301 ttcagcagat attcgactat ttacaaacgt tgagtccaag ccctggccgg attttcgacc
    78361 gtagcagcga attgtacatt attcctgatg tccgggtatt agtggacgac gataaaaatg
    78421 tacaagttat ctcaaaccga aaaaatcagc caaatatacg ctttcaagag agttacttca
    78481 aacaaatgtc acaacaagca gataaagaaa ctgaaaatta tttaaaagaa cggaaacagg
    78541 aattcgaatg gttgaagaaa acgatcctac aacgtgggga tacaattttg cgggttgctc
    78601 aagtcattgt ttcccggcaa aaagctttct ttatcgacaa ggaacgtcca atcaagcctc
    78661 ttacgttaaa ggaagttgca actgagatag atgtgcatga atccacggtc agtcgtgcag
    78721 tcaacgggaa atatttggaa acagacttcg gtgtatttga attaaaaaaa tttttcacta
    78781 cacgaattcc tacgaatagt acagaacaga cagaagacct ttcagcagat acagcgaaga
    78841 aaaagctgca ggaattagtc gatcaagaag acaaaaacaa gccgttgtct gaccagaaat
    78901 tggttgaatt gctgaaaaaa gatgagatag cgatctcaag acgaacagta gcaaaatatc
    78961 gtgatttatt aggaatcccg agttcatcaa aaagaaaacg ttatgataac taatcagtca
    79021 aaagactaga aaaagtattt cactttttct agtcttttta gttgaaaaac gagagcaaaa
    79081 ctgttagact tacagtcgtg aggttgagat ttccctttat tgatgtgaaa ttcgaatagt
    79141 taatttcttt ttttatcgta tatgggtcgt tttaagtcta cattggacgt aaagtgtcca
    79201 actaaggaga tcgctatgtt ggatgaaata aaaatgattg aatctgttgc accagatatt
    79261 atcgaagtgt tgcaagaacg ctataaaatt ttacgaaata tctattggat gcagccgatt
    79321 ggacgtcgaa gtttatcgga aagtatgggg atcacagaac gagtattgcg aacagaaaca
    79381 gatcttttga aaaatctgaa tttgatcgac acctcaaaaa gcggtatgac gctgacaact
    79441 aagggcgaag aagtttatca aagccttgaa aacttcatgg atcagctttt aggtacgcat
    79501 caaaccgaac aacagttggc agaatatttt ggtaaccaac gctgtatcgt tgtttcagga
    79561 aacagcgaag agcagaccaa agtggctgat gcattcggcg aagcgctgag cgaagcccta
    79621 gaccgactac ttccagaagg cgagaatatc atcgctgtga tgggaggaac tactatggct
    79681 gtggtagccg aacaattgtc taatctggaa aataagaaac ggcataacct gtttgtccct
    79741 gcaagaggcg ggattggtga agcgataact gtacaggcaa attctgtcag tgcaagaatg
    79801 gctgctaaag caaacggtaa ccaccgtgca ctttatgttc cggaacaatt aagtctggca
    79861 acttacaatt ctttactcaa tgagccatct atccaagaag tcttaaactt gattagtgaa
    79921 gcgaattgtg ttattcatag tattgggcgt gcattacaca tggcagcacg tcgtaagatg
    79981 actgaaaaag aattagtcat gctgaaacaa gcaaatgctg ttgcggaatc ttttggctac
    80041 tttttcaatg aaaaaggtga agtcgtttat aaggtcccta gaatcggcat agaattgcaa
    80101 gatttggaaa aagtcccgat catcatggca atcgcgggcg gaaaatcaaa agccaaagca
    80161 attcgagctt atatgaaaaa tgcaccaaaa caaacgtggc tcatcactga tgaagctgct
    80221 gcaaatgaga ttttaaaagg ggtaaccctt taaaataaat atttttttga ttttcataag
    80281 gaggaaatct taaatgacag ttaaagtagg tattaatggt tttggacgta ttggacgtct
    80341 agcattccgt cgtatccaag atgtagatgg aatcgaagta gtagcaatca acgacttgac
    80401 agatgctaaa atgttggcac acttgctaaa atacgacaca actcaaggac gtttcaacgg
    80461 aacagttgaa gttcatgaag gttcattcaa cgtaaacgga aaagaagtta aagtattagc
    80521 taaccgcaac ccagaagaat taccatgggg cgaactaggc gtagatatcg ttctagaatg
    80581 tactggtttc ttcacttcta aatcagcagc tgaaaaacat ttaacagctg gtgctaaacg
    80641 cgttgttatc tctgctcctg gcggaaacga tgttccaaca atcgtttata acacaaacca
    80701 cgaaacatta actggtaaag aaacagttat ctcaggtgct tcatgtacta ctaactgttt
    80761 agctcctatg gctaaaacat tgaacgacaa atttggtgtt gttgaaggat taatgacaac
    80821 tatccacgct tacacaggtg accaaatgac tctagacgga cctcatccta aaggtgactt
    80881 ccgccgtgca cgcgctgctg ctgcaaacat cgttcctaac tcaacaggtg ctgctaaagc
    80941 tatcggttta gtaattcctg aattgaacgg taaattagac ggagctgctc aacgtgttcc
    81001 tgtaccaaca ggttcattaa cagaattagt aacagttctt gaaaaagaag taactgttga
    81061 cgaaatcaat gcagcaatga aagaagcttc aaacgaatct tacggataca acacagacga
    81121 aatcgtttct tcagatatcg ttggtatgac ttacggttca ttatttgatg ctacacaaac
    81181 taaagtaatg acagttggcg acaaacaatt agttaaaact gttgcttggt acgacaacga
    81241 aatgtcatac actgcacaat tagtacgtac tttagaatac ttcgctaact tataagatag
    81301 acaaacgtac aagtgtaaat tatagacatg tacatttaaa agcggggaag caatcgcgct
    81361 tcttccgctt tttttgataa taagtttatt caggaggaaa taaaatggct aagaaaacag
    81421 tgaaagatat cgatttaaaa gacaaaaaag ttcttgtccg tgttgacttc aacgttcctt
    81481 tgaaagacgg cgtgatcact gacgacactc gtatcaaagc agcattgcca acaatcaact
    81541 acgttctaga acaaggcgga aaagcaatcc ttttctctca ccttggacgt gtaaaaacag
    81601 aagaagataa agaaggaaaa tctttagctc cagttgcaaa acgtctaggc gaattattag
    81661 gtaaagaagt aacattcgta cctgaaactc gcggagaaca attagaagaa gcaatccgca
    81721 acatgaatga cggcgacgtt gtcgtattcg aaaatacacg atttgaagat attgacggta
    81781 aaaaagaaag caaaaatgat acagaactag gtaaatactg ggcttcatta ggcgatgtat
    81841 ttgttaacga tgctttcggt acagctcacc gtgcacatgc ttctaacgta ggtatcgctt
    81901 caactggtat cccaacagtt gctggattct tgatggaaaa agaaatcaag ttcatcggtg
    81961 aagcagtaga agaaccaaaa cgaccaatga ttgcaatcct tggtggcgca aaagtttctg
    82021 ataaaatcgg cgtaatcgaa aacttgattc ctaaagcaga taaaatcttg atcggtggcg
    82081 gaatgactta cacattctac gaagcaaaag gaatcaagat cggtaactct ctagtagaag
    82141 ctgataaagt ggaactagca aaagaattaa tcgaaaaagc aggcgacaaa cttgtcttgc
    82201 caatcgacaa tgtctgtgca ccagaatttt caaatgatgt tgaaacacaa gtaatcgaag
    82261 gagacattcc tgatggtttg atggctttag acatcggccc agcatcagta aaattatttg
    82321 ctgatacatt acaaggtgca aaaacagttg tttggaacgg acctatgggt gtatttgaaa
    82381 tgagtaactt tgctaaaggt acaatcggtg tttgtgaagc aatcgctaac ttagaaggcg
    82441 caacaacaat catcggtggc ggagactcag ccgcagcagc agaacaatta ggatttgctg
    82501 acaaattcac acacatctca acaggtggcg gcgcaagctt ggaattgctt gaaggtaaag
    82561 aattaccagg actagcagca atcaacgata aataaacgac caagtttgct caattctgac
    82621 gagtaatcga tcaaaaggtg aagggagttt tcatgactcc ctagccattt ttcaataaat
    82681 taaggagtgt gttcctcaat gcgtaaacct attatcgcag gtaactggaa aatgaacaaa
    82741 actgcatcag aagcaaaaga atttgctgaa gcagtaaaaa acaaaatccc ttcaaatgac
    82801 aaagttgatt cagtcatcgg atctcctgca ttattcttac aagaattaat gacatcagct
    82861 gaaggtacag aactaaaaat ctcagcacaa aactgctact gggaaaactc aggtgcgttc
    82921 actggtgaaa cttcaccagc agctcttgct gatctaggtg ttcattatgt gatcatcgga
    82981 cattcagaac gtcgtgaata tttccatgaa acagacgaag acatcaataa aaaagcgaaa
    83041 gctatctttg caaatggtat gacaccaatc ctatgctgtg gcgaatcttt agaaacctat
    83101 gaagcaggca aaactgctga atggatcgaa ggacaaatta cagctggttt agctgatctt
    83161 tcagctgaac aagtgtcaaa catggttatt gcttatgaac caatctgggc aatcggtact
    83221 ggtaaatcag ctgacgcaca aatcgctgac gaaatttgtg gagttgtacg tcaaacagtt
    83281 gaaaaacttt atggaaaaga agtatcagaa gcagttcgta tccaatacgg cggttctgta
    83341 aaaccagaaa atattgctga atacatggca aaagaaaatg ttgacggtgc tttagtcggt
    83401 ggcgcaagct tgcaagcaga ttcattcttg tcattgttag acgctgtaaa ataattgttg
    83461 aaaatttgcg aaaaatgcaa gaaatgtttg cactaatgag caaaattaac taaaatcgta
    83521 tatgagggaa atcccttagg aaaaaacaaa ctcaaaggag agacaaaaca tgtcaattat
    83581 tactgatgtt tacgctcgcg agatcttaga ctcacgtggt aacccaacaa tcgaagtaga
    83641 agtatacaca gaaagcggag cttttggccg cggaatggtt ccttcaggtg cttcaactgg
    83701 tgaatacgaa gcagttgaat tacgtgacgg agacaaagct cgttatggcg gtaaaggtgt
    83761 aactaaagct gtcgacaacg taaacaacat cattgctgaa gcaatcattg gctacgatgt
    83821 tcgtgaccaa atggctatcg acaaagctat gatcgcttta gacggaactc ctaacaaagg
    83881 taaattaggt gcgaacgcta ttttaggtgt ttctatcgct gttgcacgtg cagctgctga
    83941 ctacctagaa gtacctttat accactacct aggcggattc aacacaaaag tattgccaac
    84001 accaatgatg aacatcatca acggcggatc acatgctgat aactcaatcg acttccaaga
    84061 attcatgatc atgcctgttg gcgctccaac attcaaagaa gctctacgta tgggtgctga
    84121 agtattccac gcattagctt caatcttgaa agctcgcgga ttagcaactt ctgttggtga
    84181 cgaaggtgga tttgctccaa accttggttc aaacgaagaa ggttttgaag taatcatcga
    84241 agcaatcgaa aaagctggct atgtacctgg taaagacgtt gttcttgcta tggatgctgc
    84301 ttcttcagaa ttctacgaca aagaaaaagg tgtttacgta ctagctgatt caggcgaagg
    84361 cgaaaaaaca actgacgaaa tgatcaaatt ctacgaagaa ttagtttcta aatatccaat
    84421 catctcaatc gaagacggat tagacgaaaa cgactgggac ggattcaaga aattaactga
    84481 cgtattaggc gacaaagttc aattagttgg tgacgacttg ttcgtaacaa acactcaaaa
    84541 attatctgaa ggtatcgaaa aaggaattgc taactcaatc cttatcaaag tgaaccaaat
    84601 cggtacatta acagaaactt tcgaagctat cgaaatggct aaagaagctg gctacacagc
    84661 agttgtatct caccgttctg gtgaaacaga agattcaaca atctctgata tcgctgttgc
    84721 aacaaacgct ggccaaatca aaactggttc tctatcacgt actgaccgta ttgctaaata
    84781 caaccaatta ctacgtatcg aagaccaact tggtgaagtt gcagaataca aaggtttgaa
    84841 atctttctac aacttaaaaa acaaataatt cttttctaat gaagatacat gatcgactat
    84901 tgaaagatag tcgagtatga tcacttagtt aagtaatcga actctctact tgctgaaata
    84961 tcagcaggta gagagttttt tttgcaacta gcttctgtat atgatgttta caaagaatgg
    85021 tacaagaaaa gatcatcctt gaaaaaggaa agctaacaat aactagcatc atataagagc
    85081 ttttaaggat agaacagtag aataggaatc tatgagcaag tcctctcgta taaataaaat
    85141 aggagggaag tagcagcata tttttcataa agtggctctc tttcttcatt tttactttgg
    85201 ttgctcctat ttgaaaattc gagcacactc aaatacaata aaaagaaatg gcaagggaga
    85261 ggattagatg aaaaaagaag aaaaaatcgc gattttacaa gagatcatcc gaattaaatc
    85321 agtgaatgga aacgaaggtg aagtagcagc ctatttgaat aagcttttgg caaagcatga
    85381 cataacagga gagattgttt cttacagaga tggtagagat aatttgattg ctcgttacca
    85441 aaagggccaa tcaggaaaag tgttaggcct atctgggcat atggatgtcg tggcggctgg
    85501 tgatgaatcg tcttggacct atgcaccctt tgcagcagaa atccatggga accgtttgta
    85561 cggacgtggt gctacagata tgaagagtgg cttggcagca atggtgattg ccatgatcga
    85621 actgaaagag tcaggaaaac catttaacgg aacagtcaaa ttattagcca ctgtcggaga
    85681 agaagtgggt gaactaggtg gggaacagct cacgaaagca ggctatgtag atgacttaga
    85741 tgccttgatc atcggagagc caactaatta cagtttgatg tatacgcaca tgggatcaat
    85801 caactataca gtcacctcgc acggaaaaga agcgcatagc tctatgccag accaaggtta
    85861 taatgcaatc aatcacctaa atgaattcat tacgaaagca aatgctgaaa tgaatcacct
    85921 cgctgaaaca atcgagaatc ctgtcctagg aaaaacaatc cataatgtta cattgatcag
    85981 tggcgggaat caagtcaaca gcatcccaag ccatgcgcag ctccaaggga acattcgatc
    86041 gattccagaa tacccaaatg ataaaatcat tgcattgctg caatcaatcg tcaatgaatt
    86101 gaatcaagag acagactatc atttggaatt gatgatcgat tacaacaaaa tacctgtcaa
    86161 agctgatcca gattctcctt tgattcattg cattcagcaa caattcagcc agccattacc
    86221 tttagttgga gcggcagcaa caacggatgc agccgaattt acgaaagcta accattcctt
    86281 cgactttgtc gtctttgggc ctggtgtcgt tactttgcct catcaagtag atgagtatgt
    86341 tgaaatcgat aattatttgg atatgattga aaaatatcaa ggaattattt tatcctactt
    86401 agcgtaagat ttttttaata aatgaaaaaa cagagccaga ttagcattat tttgctaatc
    86461 cagctctgtt tattcagcct atttttcttt ttgaatatat caagcgttgt ggcaacatag
    86521 tgatgactaa tgaaacaacg acgatagaca acaaacgatc aaggtagtct gtacctgcct
    86581 gtaccaagat cacacttgtt gtctgactta aacctaaccc atgtaatagc tgaacgatca
    86641 tactggatcc tgaggaggtg atcccgccaa ataactgaac ggtaatgatc gaagaaacga
    86701 tcgttccagg taccgacaaa atcaaagcga gtaataacag tttcaagctg tttttatctt
    86761 tgccgagata tcttggcaaa atcaatccag caagcaagcc tgtgagaatt tggattggcg
    86821 aataatacag agaaaaaata tctgtagtca ctccacttaa aataccgctg gacaatcctg
    86881 tcagcattcc aggtacaggc ccaagcaatg caccagcaaa aatcgttccg atagaatcga
    86941 gatagattgg caaacgaagc cataaagcaa tcgttcctcc aatataattg atcccaatac
    87001 acaaagccat cactgtaagt acgcgagttg ccattttgtt tttattcatt ttgttagccc
    87061 atctttaacc tttccaaatc tttcataatc gtttcttctt gtgcatttaa aagaacggtt
    87121 aaaaactttc ggaaaaaaag acgagtatct acttttgtca taattttact gttagcaggt
    87181 ttctgccaaa attcataacg atcaaccaat gtttgtccac gacttattcc ttgagtttcc
    87241 acagcagtga agcttttgaa accttcacag atggccccct cgataaaata agcaacagct
    87301 aacggatcat tgatgacaca tcctaaaatt ctttcttgtt tccagtggaa gtcaaaataa
    87361 aaacgagtga tcgcttttag atactctccc tcttcaggat tcatttgaca gcagtattcg
    87421 aggatagtcg gtgtcaagac gatttctctt gtcacatcta acccaaccat ttctatcgtt
    87481 tgtttgagat tctcgaatac ataagaagct gcttcaggat cacaccagta attgtattca
    87541 gcaactggcg aacagttacc atggcttttg taagttccac ccatcgagac aaaacgatcc
    87601 atatgtttcc caatatttgg gttgactttc aaagcaaaag cgatattggt caaaggaccc
    87661 aaagcaatga ctgatgtatt tgtcttttct ttgaaataat cagccaaaaa atcaacggca
    87721 tggagaggtt ctgcctgttt tctcaagatc atcgggaaat ttgtttcccc taaaccatcc
    87781 ataccatgag tgtcttgtgc actgatgaaa ggttgcttca aaggtttttc cattccttga
    87841 taaacaggaa tgtctaaacg atcacagcgt tccaggcatt tcagcacatt ctgcgtacct
    87901 atatctgcag ggacattccc acaaactacg gtaatagcaa ctacttcgat atcaggggat
    87961 ttgacagcta atgataaagc taaagtgtcg tcgatccctg gatcgcaatc aataattact
    88021 tttcgcatta tttccaatct cctttcttta gagatactta gttttttatt ctgggaagtt
    88081 tccgaaccag tccaagacga gcgtcttgat tataatgcac gaaattagta tactggcttt
    88141 tattgcgaat gtaaatgctc tcttacaagt aaatctattg aggagaactt taagtaaatg
    88201 atacaatgaa aaagaaatgg aatttacgtg tgccgaaaat aaagaatcct atggatcggt
    88261 acattggata atagttagat tatagtttgg cagaaagcgg cgggtgtaca atggttattt
    88321 tttatttcat tcttattctg ataattatag ggatcagtta ccattttctt tcttattttt
    88381 atgcaaagaa attagtaaga gcgggagtac agagaggata tgactggtat gaagacactg
    88441 gtcatcgttt gatggaccct gctgcagtca cgtcagtaga aaaaaaacga caaaaattag
    88501 aagaagagtg tgaaagcttt tgggagcagg gaacacccaa aacaatcaaa agttatgacg
    88561 ggttgaaact agctggtcag atgttcatcc atccaaataa gcagaataaa tgggtcattt
    88621 gcgtacatga ttaccgaagc acagggaaaa gagacatgtc tcatataggt aaaagatatg
    88681 cagagaaagg attcaacgtt cttgtacctg atttacgtgc gcacggagaa agtgaaggag
    88741 aaatcatcgg aatgggatgg ctagatcggc tagatttaat tgcctggatc caactcatct
    88801 tagatgaaca gccgaatgcg tccattattt tacacggcgg atcaatggga gcttctacga
    88861 tcatgatggc aagtggtgaa aaacttccat cagcggtcaa aggatttatt ttagacagcg
    88921 gatatgtttc tgtttatgca gaattccgat atatgttaag taaaatcact gtctttccta
    88981 aaaaaatggt catgagatat gcgaatcatt atgcccagaa atacgccggt tactccttaa
    89041 agcaagcatc agctacgaga cagcttggca gcaaccatct gcctttattg gtcattcatg
    89101 gtgaaagaga tcattttgtc cctacagaag cagcgtacac gattcaaaat gctacggcag
    89161 gggataaagc actgctgctt gttccagaag cagaacactt ggaagcatcg atgaaagatc
    89221 cgaaaaccta ttggacagtg attttttcat ttattgaaca aagaataacg atgaattaat
    89281 tttctcttct tttttgtaag taattcgagg ataaataatt gtttatagat caggagactg
    89341 ctaaaataag gtaggaataa aaaaatatca gttatttgag gagtgcggca tatggaaatc
    89401 aaagaagaaa aaaatcgtct tgctttattc aatgatgaac aacaagagat cggagaaatg
    89461 acatggtcag atgcgggccc agacatcatg atcattgacc atacatttgt agatccagca
    89521 tatcgtggtc aaaagttagc tgaaaagcta gtgtatactg gagtagaatt agcaagacga
    89581 gacgggaaaa agatcattcc tttatgccct tatgcaaaaa aagagtttga aaagaaacca
    89641 gaatatcaag acgtcttacg tcagtcataa gaataacagc tgaaagtttt gaactttcag
    89701 ctgttattcc atttatatag agttgtcacg gacttttatc tatgataaag ttaaagagac
    89761 gatatctttt gggaaggagg ctgcgatatg tataatttaa ttttgggaat cgtgatcgtt
    89821 ttatcgattg ttatggtcat tgcgattatg atgcagccaa gcaagcaaaa tagtgcagcg
    89881 agtgcattta ccggcggtgc ggatcaacta ttcggcaaac aaaaagctcg cggctttgaa
    89941 gctgtcatgc agcgatcaac tgcagttatg ggagctatct ggatgatctt attatttgtc
    90001 ctagcatatt tatcttcgaa gtagttgaat aagcctctcg caaaacggcg ggcggctttt
    90061 tttattgcaa agagaacgct taagaagaag ttatgaaaga aactatggta aaatgaggcg
    90121 aaagaggtga gacgagtgag acaattacca aaaccccttt atgcaaaaca tggaaagcga
    90181 gcggttttgc tgcttcacgc ttactcagga agtccaaacg atgttcgaat gctcgcacga
    90241 tttttggaaa aagcagatta tacagtttat gcgccgcttt ttaaaggaca cggaacaatg
    90301 gagccatatg atattttgca agaaaaagca gaaagctggt gggcagacac taaaaaagcc
    90361 atccattttt tacagtctga gcagttttct gatattgccg tattaggatt atcaatgggc
    90421 ggcatctttg ctgtacgagc actagaagaa gaatcagtga ttggcggcgg ttttttctgt
    90481 tcgcctttgt ctcctgtaaa aacaaacgtg cctgaaaact ttgaaaaata cgtacgtcaa
    90541 gtgctgaaga cagcaggtaa atcagagaaa gaaatcaatg aaaaagcagt tgcttaccgc
    90601 tcgttagctg aacaacaatt aatggatata caagatcagg cagcaatcgt agaaagtagg
    90661 ttgtcagata tccaacagcc gatattttta gctcaagctg ggaaagatga aatgatcgac
    90721 cctaatggcg tatttgaaac tgcacgcaag ttgagccgtc agcgagtgac acttcgatgg
    90781 tatcctgaaa gtggtcatgt gattacagtt ggtacagcaa gacgcgaatt agaaaaagac
    90841 gtcttggaat ttttagaaaa attaccttgg aatgaggaat aaatatgaca aaagaaacga
    90901 taaaagagaa aattctcttt ttcatggaaa gcagtcgcaa acgcagtttt tctatggaag
    90961 aaatcgctga aggtttaggg ttccaaaaaa gcgatgattt taaattacta gtacaaacga
    91021 ttgctcaaat ggaaagagaa cagagtatag ttttcaataa aaaaggaaaa gtgaaacttc
    91081 ctttaaaacc aatcttgatc gaaggaacat ttcgagcaaa tgaacgagga tttggatttg
    91141 taacgattga tcccgaagaa gatgacatct atatcccaaa agaagcgact ggttttgcaa
    91201 tggatggaga caccgtagca atcgatatca tgaagcgagc aaatacagat atggatcgcg
    91261 gagcagaggg gaaagtagtc gagattcgag accggtcgac tactcagtta gcaggggaat
    91321 tcgttgctta ttcagaagaa gaaatacaag aaacggatct ttacggcgtt gttatcccga
    91381 aagatagaaa gctcaaccaa tttaaagtat atgcagcagc agagggagtt cgtccagttg
    91441 acggaagtat tgtgctgatc gaactgaccc attaccctga aaaagggtat gcgactagtt
    91501 tagaagggat aatcaagcaa gtgattggtc ataagaatga ccctggcatg gatatcctct
    91561 caattgtcgt ggctaatggt atccctacga aatttcctga tgatgtattg tctgaagcag
    91621 atgaagttcc agatcaaatc agcgaaaagg atataactgg acgtagagat ttaagaaatc
    91681 agctcattgt tacgattgat ggagaagatg caaaagatct agatgatgca gttacggtta
    91741 aaaaattaga taacggtaat tattttcttg gagtacacat tgcagatgtt tcttactatg
    91801 tgacacaagg cagtcaattg gacagagaag catatgaaag agggacaagt gtatatttga
    91861 ctgatcgagt gattccgatg atcccgcaac gtttatcgaa tggtatctgt tcactgaatc
    91921 cccatgttcc aaggctgacc atgagttgcg aaatggagat tgatccaaat ggacaagtag
    91981 tctctcatga tattttccca agtgtgatcc aaacaacaga acggatgacc tacacagctg
    92041 tcaatcaaat cttagaagat caagacgaag aaacgatgca acgctatgct cagctcgtac
    92101 ctatgttcca agaaatgggt gaattgcatc gaatccttga aacgatgcga atcaaacgtg
    92161 gcgcgatttc ttttgaggat cgcgaagcaa aaatccttgt tgatgctgaa ggacacccaa
    92221 atgatatcca gctaagaagc agaggaattg gtgaacgctt gatcgaatct ttcatgcttg
    92281 cggccaatga aacggtagct gaacattata ataaattaaa tctgccgttt atttaccgaa
    92341 tccatgaaca gccaaaagaa gaaaaaatgc agcgcttctt tgattttgct tcagctttgg
    92401 gcattctggt ccgaggaacg aaaggaacga tcacaccaaa agatttacag catgtgatcg
    92461 aaggagtaga agacaaacca gaagctgcag ttatcaatac tatgttgcta agaagcatgc
    92521 aacaagcacg ctattcagaa gacaattttg ggcattatgg cttagcggca gattactaca
    92581 cacactttac atctcctatt cgacgctatc ccgatctaat cgtccatcgt ctgatccgtt
    92641 cttacagtga agataaaagt gaaagcaatc aaagttcgtg ggctacggaa cttcctgaaa
    92701 ttgcagatca tagttctaag atggaacgtc gtgcagtaga aacagaacgt gaagtagatt
    92761 ccatgaagaa agcagaattc atgatggaca aagtggggga agaattcgat gggatcatca
    92821 gctctgttgt gaaattcggc ctgtttatcg aactgccgaa tacagtagag ggattgatcc
    92881 atatcaatga attgaaacag gactacttcc attttgttga aaatcattta gctcttgtag
    92941 gtgaaagaac tggtctgact tttaaaatcg gacagaaagt gcgagtaaaa gtagtcaaag
    93001 ctgatccaga tgaacgagca gtagattttg aacttgtttc cgcagaagag gttacagcgt
    93061 tggaacggcc aaaaacacga aatggaaaat caggaaaacg cgaaggaaaa caagcaggaa
    93121 aacgcccaga aagaaatggc aagaagaaca aacgatatag cgatagtaag caaacgttcg
    93181 gtaagaaaaa agggaagaag ccattttaca aaggtgttaa aaagaagaaa aagaaaaaat
    93241 aaaatgagtt ggaggaaaca gctatgccaa aaggcgaggg aaaattaatt gcacaaaaca
    93301 agaaagctcg ccatgattat tcgatcatcg acacgatgga agcagggatg gtcttgcaag
    93361 gaaccgagat caagtcgata cgaaacagcc ggatcaatct aaaagatgga tttattcgcg
    93421 tccgcaacgg agaagctttc ttgcataatg ttcatatcag tccttatgaa caaggaaata
    93481 tttttaatca tgatccgttg cgcacgagaa agttattatt acacaaaaaa caaatcatcc
    93541 ggcttgaaaa tgaattgaaa aatactggaa tcactgttgt tcctttaaaa gtctatattc
    93601 gtaacggcta tgccaaggta ttgattggtc tggcgaaagg gaaaaaatct tatgataaac
    93661 gggaagattt gaaacgaaaa gatatcgatc gacaaattga tcgaacatta aaaaatttct
    93721 ctagataatt tcattatcct ttttcgcata ttcggtgctt ttgcttttac ttaatgataa
    93781 aatttgttag catttaatct gataaaacat gattatcata ggttttttct tagatcagga
    93841 gcaccgtgta taaagaaaaa ttcgcttcgt ttttgactga aataaaaaag tgtctaattt
    93901 tcatgaaaat gttttgaatt ttctgtttca tggtggtatg ataccgtgtg gttagaaacc
    93961 gattggaaaa aaccagatac gcacatgaat tgattttatg tgaatttcgg ggaaaagagg
    94021 tgaaatggat tggatgaacg ctcgctaacg ttccacattg gaccaatttg gtttgatggt
    94081 acagtttgta taatggtgtt actgacctgt ttgattgttt tcttcttagc ttactttttt
    94141 acaagaaatc ttaagttgaa accaacaggc aaacaaaatg cgatagaatg ggtcattgat
    94201 tttagtcgag gaatcgtaac agataatctg cctagaaaag aattgagcaa tttccatttg
    94261 ttggcattta cattgttttt atttgttttt gttgcaaaca acatcggttt agtaacaaaa
    94321 atcgttttac ctaacgaaac aacactttgg aaaagcccga cagcagaccc gtttgttact
    94381 ttaactttgg catttataat gatcttactt actcacttat ttggggtgaa aaagttaggc
    94441 ttcaaaggat atttcgtaaa ttcgttcttg aaaccatata gttttatgtt tccgatgaaa
    94501 ctgattgaag aatttacaaa cttattaaca cttgctttac gtctttatgg gaatatttat
    94561 gcgggggaag ttttgctaac cttgattgca aaaatggtaa taagcttagg ctggttctca
    94621 ttgccgttag caattccatt agagatggtc tggatcgcat tctccttgtt tatcggaagc
    94681 atccaagcat ttgtcttcgt tactttatct atggtttata tgagccataa gattgaagtg
    94741 gaagaataat attcgtttta aaaacaaact actatttatc taatttagga ggaaaacaat
    94801 tatgaattat atcgcagcag caatcgcaat catgggagca gcaatcggag ccggttatgg
    94861 taacggtcaa gttatctcta aaacaatcga atcaatggct cgccaacctg aaatgtctgg
    94921 tcaattccgt acaacaatgt ttatcggggt agctctagtc gaagctgttc ctatcttagg
    94981 ggtagttgtc gccttgattc tagtttttgg tgtttaattg aatcagtatt aaggcagaaa
    95041 caatttggtt tgttactgct tttttacatg aaaactagaa gtacttcaaa gaaaggaagg
    95101 ctcgcctatg ctgaatcaat tggcaattgc agaagttggc aatgcgatgt taggaaatgt
    95161 catcgttgtc agcggctcat ttttgatact actggctctc ttgaagcatt ttgcttgggg
    95221 accaatcagc gatattttga aaaaacgtga agacaagatc gccaatgatt tggattctgc
    95281 agaacaatct cgcatcaact cagcgaaaat ggaacaagaa cgcgaacaac aattgttagc
    95341 ctctcgttct gatgcagctg atatcatcaa aaatgcgaaa gaaagtggag aattaagccg
    95401 ccaaaatatt ttgaaggatg ctcaagaaga agcagctcgt ctaaaaagta aagcccaagc
    95461 tgatatcact gtagaacgtg attcagcgct gaactctgta aaagacgatg ttgcagaact
    95521 atctcttcaa atcgcggaaa aaatcttgaa caaagaatta tctccagaaa tgcacgaatc
    95581 attaatcaat caatatatcg aaggtctagg ttcttcaaat gaaactagat aaatatacag
    95641 taggaagacg ttacgggaaa gctttatttg aactggcgat cgattcaaat aaagcagaag
    95701 aaatttatca agatctcgta agcttacgtc aaatctatga acaagtacct ggattaggta
    95761 atatgttaag tgatgttcgt ctagaacctc atgaaaaacg agaaatcatg gacaagcttg
    95821 ttagtggcta tgatggaatc gttaaaaact ttttagaagt tgtttatgcg tataaccgaa
    95881 tggatgatct tttgttcatg atcgaagaat atgaacgtcg atatgatgaa catcaaggtt
    95941 tgctgttggg aagtgtcaca acagcagttc cactttcaga cgaacaacga atacaattag
    96001 aaaagaatgt tgcaaaaaca atgaactatc aatcagtcga attaaaacaa atcgttgatt
    96061 cttctattat tggtggcgcg attgttgaag ctaaccatcg agtaatcgac ggaagcattc
    96121 gcacgcaatt agaaaatatg cgtaatcaat taaatagata gtacaaagag gtaggtgaat
    96181 cgaatggcca tcaaagcaga agaaattagt gccttgatta aagaacaaat tgaaaattat
    96241 caaaacgtgc tttcagtcga agaaatcggt accgtaactt atgtaggtga cgggatcgct
    96301 cgtgcacatg gtttagaaaa cgcgatgagt ggtgagttgc ttgaattttc aaatggctca
    96361 tacggaatgg cgcaaaactt agaatcaaat gatgtaggta tcattatcct tggcgatttc
    96421 gagtccattc gtgaaggaga taaagtaaaa cgaacaggaa agatcatgga agttccagtt
    96481 ggagaggcct tgattggacg ggtagttaat ccgctaggtc agccaattga cggactaggt
    96541 gaaatcgtta ctgacaaagc tcgtccagta gaagcaatgg caccaggcgt tatgcaacgt
    96601 aaatctgtta acgaaccaat gcaaacaggt ctaaaagcaa tcgacgctct tgtaccaatc
    96661 ggacgcggcc aacgtgaatt agttatcggt gaccgtaaaa cagggaaaac ttccattgcg
    96721 atcgatacga tcatcaacca aaaaggtcaa gatatgatct gtatctatgt agcaatcgga
    96781 caaaaagatt ctacagttcg tacacaagtt gaaacattga aaaaatatgg cgcaatggat
    96841 tacacaatcg ttgtgaatgc cggtgcgtct caaccagcgc cgttgcttta tatcgcacca
    96901 tatgctggta ctgcaatggg tgaagagttc atgtacaacg ggaaacatgt cttgatcatt
    96961 tttgatgatc tttcaaaaca agctgttgct tatcgtgaac tttccttgct gcttcgccgt
    97021 ccaccaggtc gtgaagctta tccaggggat gttttctact tacactcacg cttattggaa
    97081 cgtgcagcaa aattaagtga tgatttaggt ggcggttcaa tgactgcctt accatttgtt
    97141 gaaacacaag ctggagatat ctctgcttat atcccaacaa acgttatttc gatcactgat
    97201 ggacaaatct tcttagaaag cgacttgttc tacgcaggta ctcgtccagc ggttgatgcc
    97261 ggactatctg tatctcgtgt agggggttct gcgcaaatca aagcaatgaa aaaagttgct
    97321 gggactttac gtctagactt agcaagttac cgtgaattag aagcatttac acaattcggt
    97381 tctgacttgg atgcagcaac acaagcaaaa ctaaatcgtg gacgccgtac agtagaaatc
    97441 ttgaaacaaa aattgcatgc accgctagct gttgaaaaac aagtagtgat tttgtatgct
    97501 ttgacacatg gattcttaga tagtatccct gtagacagta ttttagactt cgaacatgaa
    97561 ttattcgaat atctggatac caatcattca gatatctttg aaacaatccg tacaacaaaa
    97621 gatcttccag aagaagaagc gttgaacagt gcgattcagg aatataaaga catgttctta
    97681 gctacaaaag gaaataattc ctctacagaa gataaattga aatccattca aaatgcatag
    97741 tgaggtgaga tgaatgggtg cttcattaaa tgaaatcaag acacgtatcg cttcaacgaa
    97801 aaaaacgagc caaatcacgc gggcaatgca gatggtgtct gcttctaagt tgacaaaatc
    97861 tgaagcttct tcacagaagt ttcaggttta tgcaacaaaa gtaagagaaa tcgttacgca
    97921 tttgactgct actcagttaa atgatattgc ctcagaaaat cctagagggg atatcaatta
    97981 caacagcatg ttgctcagcc gtcctgttaa gaaaacgggt tatatcgtca tcactgcaga
    98041 tggcggttta gtcggtggat ataacagctc gatattgaaa caaacgatgt ctattttaga
    98101 agaagaccac caatcacccg atgattacgt gatgatcgca attggcggaa ctggtgcaga
    98161 cttctttaaa gcacgcggta tcaatctagc atatgagctg cgcaatctat ctgatcagcc
    98221 aagctttgat gaagtccgca aaatcgtaaa tatggcaaca actatgtacc aaaacgaagt
    98281 attcgatgaa ttatacgtat gttataacca tcatatcaat tcgctaacta gtcagtttcg
    98341 tgtagaaaaa atgctgccaa tctcagactt agatccagaa gaagcaacta ctttcgaaca
    98401 agaatatatt tttgaacctt caaaagaaga aatcttgtct cagttattgc ctcagtatgc
    98461 tgaaagtttg atctacggtg cgatcgttga cgccaaaaca gcagaacatg ctgctggtat
    98521 gacagcaatg aaaacagcaa ctgataatgc tgctaatatc attgatgagc tgacggtttc
    98581 ttacaaccgt gcaagacaag gggctattac ccaagaaatt accgaaattg ttgccggagc
    98641 ttcggcacta gaatagtatt gagattggag gaaaaaagat gagttcaggc aagattgttc
    98701 aagtaatcgg tcccgttgtc gacgtggaat tttctttaga ccaatcctta ccagatatca
    98761 acaacgcatt agttgtttat aaaaatgacg aaaataaatc aaaagttgtt cttgaagcag
    98821 cgttagaatt aggggacgga gtgatccgga ccattgcgat ggaatcaaca gatggtttac
    98881 aaagaggaat ggaagtcatt gatacaggca aagcaatctc tgttcctgta ggtaaagaaa
    98941 cattaggtcg cgtattcaac gtactaggag atacgatcga tttagaaaca cctttcccag
    99001 aagatgcgga aagaagcgaa attcataaaa aagcaccagc ctttgacgaa ttaagtacaa
    99061 gtacagaaat tttggaaaca gggatcaaag ttatcgattt gcttgcccca tatttaaaag
    99121 gtgggaaagt cggactattc ggtggtgccg gtgttggtaa aaccgtactg atccaagaac
    99181 tgatccataa tatcgcccaa gaacatggtg gtatttctgt atttaccggt gtaggtgaac
    99241 gtactcgtga aggtaatgac ttgtattatg aaatgaaaga ttcaggagtt atcgaaaaaa
    99301 cagccatggt gttcggacaa atgaacgaac caccaggtgc acgtatgcgt gttgctttga
    99361 ctggtttgac gattgcggaa tatttccgtg atgtagaagg tcaagatgta ctgttgttta
    99421 tcgacaacat tttccgtttc actcaagctg gatctgaagt atcagccttg ttaggacgga
    99481 tgccttctgc ggttggttat caaccaacat tggcaacaga aatgggtcaa ttgcaagaac
    99541 gtatcacatc tacgaaaaaa ggttcgatca catcgatcca agcaatctat gtgccagccg
    99601 atgactacac tgacccggcg ccggcaacag cattcgccca tttggatgcg acaaccaact
    99661 tggaacgtaa attaacagaa caagggattt atccagcggt agatccattg gcttcttctt
    99721 caagtgcttt agcacctgaa atcgtgggag aagaacatta taaagtagca acagaagtcc
    99781 aacacgtatt gcaacgatat cgtgaattac aagatatcat tgctatctta ggtatggatg
    99841 aattatccga tcaagaaaaa gtgttagttt ctcgtgcccg cagagtccaa ttcttcttgt
    99901 cacaaaactt taacgtggct gaacaattta ctggtcttcc aggttcatat gtccctgtag
    99961 aagaaacagt gaaaggattc cgcgaaatct tggaaggcaa atacgatgat cttcctgaag
   100021 aagctttccg aagtgttggc cgaatcgaag acgtcgtaga aaaagcgaaa acactaggct
   100081 actagaaagg ggtgccccat ggatcaatat ttaacagtaa atgtggtaac tcctgccggt
   100141 ttggtctatg atcaccatgc agctatcgtc gttgcacgta cgacagctgg tgagcttggg
   100201 attttgccta aacatgcacc aatcatcgtt ccattgacga ttgatgaagt tcgtgtaaaa
   100261 cgaacagatt cagataccca cgtcgattgg atcgctgtta atggcggaat catggaagtt
   100321 cgtgataatg ttgtttcaat cgttgctgat agtgcggaac gtgaacgtga tatcgatgta
   100381 tctcgtgcgg aacgtgcaaa acaacgcgca gaacgccaaa tcgaagaagc aaaagcaaaa
   100441 caagatacga acgaattgaa acgggcaact gttgcgttgc accgagcaat caatcgtatc
   100501 aaagtatcta agcactcata aaatgagatg cggatgttta tccgtgttta agaacagtaa
   100561 aaagaggtta tgacagaagt ggtcagcttc aagacataag aaggaattta cgaaaattgc
   100621 ttctcaaatt tttgtgaaat ctcagcttat ttttcgaaga agctacttct ggaacaccgt
   100681 atatcggagg ttgtgaaatc agcgttttgt cctgagtatt agagaaacaa atggaaaata
   100741 gttcctcata ttttgccgtt ttttgagcta ataccgaggg tcagatgatt gaacaccgtt
   100801 tatttggaat aagggactgc gacaagac
//
LOCUS       DAFBQH010000002        98736 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.002, whole genome shotgun sequence.
ACCESSION   DAFBQH010000002 DAFBQH010000000
VERSION     DAFBQH010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 98736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 98736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..98736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.002"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..106)
                     /locus_tag="LK694_000097"
     rRNA            complement(<1..106)
                     /locus_tag="LK694_000097"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            847..1536
                     /locus_tag="LK694_000098"
     CDS             847..1536
                     /locus_tag="LK694_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5051001.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1523..2731
                     /locus_tag="LK694_000099"
     CDS             1523..2731
                     /locus_tag="LK694_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK5051002.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            3025..4296
                     /gene="serS"
                     /locus_tag="LK694_000100"
     CDS             3025..4296
                     /gene="serS"
                     /locus_tag="LK694_000100"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBK5051003.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4845..6329)
                     /gene="guaB"
                     /locus_tag="LK694_000101"
     CDS             complement(4845..6329)
                     /gene="guaB"
                     /locus_tag="LK694_000101"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBK5051004.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6834..7529)
                     /locus_tag="LK694_000102"
     CDS             complement(6834..7529)
                     /locus_tag="LK694_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBK5051005.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLTPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7619..8719)
                     /gene="ychF"
                     /locus_tag="LK694_000103"
     CDS             complement(7619..8719)
                     /gene="ychF"
                     /locus_tag="LK694_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBK5051006.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8728..8919)
                     /locus_tag="LK694_000104"
     CDS             complement(8728..8919)
                     /locus_tag="LK694_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBK5051007.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8940..9830)
                     /locus_tag="LK694_000105"
     CDS             complement(8940..9830)
                     /locus_tag="LK694_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBK5051008.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9817..10584)
                     /locus_tag="LK694_000106"
     CDS             complement(9817..10584)
                     /locus_tag="LK694_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK5051009.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10596..11309)
                     /gene="rsmG"
                     /locus_tag="LK694_000107"
     CDS             complement(10596..11309)
                     /gene="rsmG"
                     /locus_tag="LK694_000107"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBK5051010.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11541..13442)
                     /gene="mnmG"
                     /locus_tag="LK694_000108"
     CDS             complement(11541..13442)
                     /gene="mnmG"
                     /locus_tag="LK694_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBK5051011.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEVQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13459..14856)
                     /gene="mnmE"
                     /locus_tag="LK694_000109"
     CDS             complement(13459..14856)
                     /gene="mnmE"
                     /locus_tag="LK694_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBK5051012.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            15155..15538
                     /locus_tag="LK694_000110"
     CDS             15155..15538
                     /locus_tag="LK694_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051013.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15706..16464)
                     /locus_tag="LK694_000111"
     CDS             complement(15706..16464)
                     /locus_tag="LK694_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HBK5051014.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16482..17300)
                     /locus_tag="LK694_000112"
     CDS             complement(16482..17300)
                     /locus_tag="LK694_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBK5051015.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17297..17653)
                     /gene="rnpA"
                     /locus_tag="LK694_000113"
     CDS             complement(17297..17653)
                     /gene="rnpA"
                     /locus_tag="LK694_000113"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBK5051016.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17771..17905)
                     /gene="rpmH"
                     /locus_tag="LK694_000114"
     CDS             complement(17771..17905)
                     /gene="rpmH"
                     /locus_tag="LK694_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBK5051017.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18428..19762
                     /gene="dnaA"
                     /locus_tag="LK694_000115"
     CDS             18428..19762
                     /gene="dnaA"
                     /locus_tag="LK694_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBK5051018.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19960..21090
                     /gene="dnaN"
                     /locus_tag="LK694_000116"
     CDS             19960..21090
                     /gene="dnaN"
                     /locus_tag="LK694_000116"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBK5051019.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21319..21561
                     /gene="yaaA"
                     /locus_tag="LK694_000117"
     CDS             21319..21561
                     /gene="yaaA"
                     /locus_tag="LK694_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBK5051020.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21548..22672
                     /gene="recF"
                     /locus_tag="LK694_000118"
     CDS             21548..22672
                     /gene="recF"
                     /locus_tag="LK694_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBK5051021.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22669..24615
                     /gene="gyrB"
                     /locus_tag="LK694_000119"
     CDS             22669..24615
                     /gene="gyrB"
                     /locus_tag="LK694_000119"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBK5051022.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24635..27106
                     /gene="gyrA"
                     /locus_tag="LK694_000120"
     CDS             24635..27106
                     /gene="gyrA"
                     /locus_tag="LK694_000120"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBK5051023.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDRAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27342..27641
                     /locus_tag="LK694_000121"
     CDS             27342..27641
                     /locus_tag="LK694_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBK5051024.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27691..28203
                     /gene="ssb"
                     /locus_tag="LK694_000122"
     CDS             27691..28203
                     /gene="ssb"
                     /locus_tag="LK694_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK5051025.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            28229..28465
                     /locus_tag="LK694_000123"
     CDS             28229..28465
                     /locus_tag="LK694_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBK5051026.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28608..30581
                     /locus_tag="LK694_000124"
     CDS             28608..30581
                     /locus_tag="LK694_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBK5051027.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30587..31039
                     /gene="rplI"
                     /locus_tag="LK694_000125"
     CDS             30587..31039
                     /gene="rplI"
                     /locus_tag="LK694_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBK5051028.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31313..32680
                     /gene="dnaB"
                     /locus_tag="LK694_000126"
     CDS             31313..32680
                     /gene="dnaB"
                     /locus_tag="LK694_000126"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBK5051029.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNIIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33460..34752
                     /locus_tag="LK694_000127"
     CDS             33460..34752
                     /locus_tag="LK694_000127"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBK5051030.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35539..36402
                     /locus_tag="LK694_000128"
     CDS             35539..36402
                     /locus_tag="LK694_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK5051031.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36531..38153)
                     /locus_tag="LK694_000129"
     CDS             complement(36531..38153)
                     /locus_tag="LK694_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5051032.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38402..38923
                     /locus_tag="LK694_000130"
     CDS             38402..38923
                     /locus_tag="LK694_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBK5051033.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(39109..40839)
                     /gene="spxB"
                     /locus_tag="LK694_000131"
     CDS             complement(39109..40839)
                     /gene="spxB"
                     /locus_tag="LK694_000131"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBK5051034.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            41004..41822
                     /locus_tag="LK694_000132"
     CDS             41004..41822
                     /locus_tag="LK694_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBK5051035.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41925..42122)
                     /locus_tag="LK694_000133"
     CDS             complement(41925..42122)
                     /locus_tag="LK694_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051036.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            42291..42869
                     /locus_tag="LK694_000134"
     CDS             42291..42869
                     /locus_tag="LK694_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBK5051037.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            42964..43101
                     /locus_tag="LK694_000135"
     CDS             42964..43101
                     /locus_tag="LK694_000135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051038.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(43914..44417)
                     /locus_tag="LK694_000136"
     CDS             complement(43914..44417)
                     /locus_tag="LK694_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBK5051039.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44775..45800
                     /locus_tag="LK694_000137"
     CDS             44775..45800
                     /locus_tag="LK694_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK5051040.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            46123..47001
                     /locus_tag="LK694_000138"
     CDS             46123..47001
                     /locus_tag="LK694_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HBK5051041.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            47477..47593
                     /locus_tag="LK694_000139"
     CDS             47477..47593
                     /locus_tag="LK694_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBK5051042.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            48521..48859
                     /locus_tag="LK694_000140"
     CDS             48521..48859
                     /locus_tag="LK694_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051043.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48953..49183
                     /locus_tag="LK694_000141"
     CDS             48953..49183
                     /locus_tag="LK694_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051044.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            49583..49954
                     /locus_tag="LK694_000142"
     CDS             49583..49954
                     /locus_tag="LK694_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBK5051045.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(50228..50920)
                     /locus_tag="LK694_000143"
     CDS             complement(50228..50920)
                     /locus_tag="LK694_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK5051046.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(50923..51708)
                     /locus_tag="LK694_000144"
     CDS             complement(50923..51708)
                     /locus_tag="LK694_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK5051047.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            51945..53345
                     /gene="lacG"
                     /locus_tag="LK694_000145"
     CDS             51945..53345
                     /gene="lacG"
                     /locus_tag="LK694_000145"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBK5051048.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            53370..53687
                     /locus_tag="LK694_000146"
     CDS             53370..53687
                     /locus_tag="LK694_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBK5051049.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            53700..55373
                     /locus_tag="LK694_000147"
     CDS             53700..55373
                     /locus_tag="LK694_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBK5051050.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            55387..57018
                     /locus_tag="LK694_000148"
     CDS             55387..57018
                     /locus_tag="LK694_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HBK5051051.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(57509..59074)
                     /gene="guaA"
                     /locus_tag="LK694_000149"
     CDS             complement(57509..59074)
                     /gene="guaA"
                     /locus_tag="LK694_000149"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBK5051052.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(59133..60056)
                     /locus_tag="LK694_000150"
     CDS             complement(59133..60056)
                     /locus_tag="LK694_000150"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBK5051053.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            60252..60857
                     /locus_tag="LK694_000151"
     CDS             60252..60857
                     /locus_tag="LK694_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK5051054.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            61165..62181
                     /locus_tag="LK694_000152"
     CDS             61165..62181
                     /locus_tag="LK694_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBK5051055.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            62322..63623
                     /locus_tag="LK694_000153"
     CDS             62322..63623
                     /locus_tag="LK694_000153"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBK5051056.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            63669..64331
                     /gene="deoC"
                     /locus_tag="LK694_000154"
     CDS             63669..64331
                     /gene="deoC"
                     /locus_tag="LK694_000154"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBK5051057.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIVETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            64352..64750
                     /locus_tag="LK694_000155"
     CDS             64352..64750
                     /locus_tag="LK694_000155"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBK5051058.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            64816..65898
                     /locus_tag="LK694_000156"
     CDS             64816..65898
                     /locus_tag="LK694_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK5051059.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            66110..67672
                     /locus_tag="LK694_000157"
     CDS             66110..67672
                     /locus_tag="LK694_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5051060.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            67665..68798
                     /locus_tag="LK694_000158"
     CDS             67665..68798
                     /locus_tag="LK694_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5051061.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            68798..69754
                     /locus_tag="LK694_000159"
     CDS             68798..69754
                     /locus_tag="LK694_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5051062.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            69853..71019
                     /gene="deoB"
                     /locus_tag="LK694_000160"
     CDS             69853..71019
                     /gene="deoB"
                     /locus_tag="LK694_000160"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBK5051063.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            71043..71861
                     /locus_tag="LK694_000161"
     CDS             71043..71861
                     /locus_tag="LK694_000161"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBK5051064.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            71878..72585
                     /gene="deoD"
                     /locus_tag="LK694_000162"
     CDS             71878..72585
                     /gene="deoD"
                     /locus_tag="LK694_000162"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBK5051065.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(72883..73569)
                     /gene="gpmA"
                     /locus_tag="LK694_000163"
     CDS             complement(72883..73569)
                     /gene="gpmA"
                     /locus_tag="LK694_000163"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBK5051066.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(73741..74322)
                     /locus_tag="LK694_000164"
     CDS             complement(73741..74322)
                     /locus_tag="LK694_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5051067.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(74455..75138)
                     /gene="rpiA"
                     /locus_tag="LK694_000165"
     CDS             complement(74455..75138)
                     /gene="rpiA"
                     /locus_tag="LK694_000165"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBK5051068.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            75457..75870
                     /gene="rpsL"
                     /locus_tag="LK694_000166"
     CDS             75457..75870
                     /gene="rpsL"
                     /locus_tag="LK694_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBK5051069.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            75954..76424
                     /gene="rpsG"
                     /locus_tag="LK694_000167"
     CDS             75954..76424
                     /gene="rpsG"
                     /locus_tag="LK694_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBK5051070.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            76518..78605
                     /gene="fusA"
                     /locus_tag="LK694_000168"
     CDS             76518..78605
                     /gene="fusA"
                     /locus_tag="LK694_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBK5051071.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            78777..79964
                     /gene="tuf"
                     /locus_tag="LK694_000169"
     CDS             78777..79964
                     /gene="tuf"
                     /locus_tag="LK694_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBK5051072.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            80371..80679
                     /gene="rpsJ"
                     /locus_tag="LK694_000170"
     CDS             80371..80679
                     /gene="rpsJ"
                     /locus_tag="LK694_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBK5051073.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            80710..81339
                     /gene="rplC"
                     /locus_tag="LK694_000171"
     CDS             80710..81339
                     /gene="rplC"
                     /locus_tag="LK694_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBK5051074.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            81367..81990
                     /gene="rplD"
                     /locus_tag="LK694_000172"
     CDS             81367..81990
                     /gene="rplD"
                     /locus_tag="LK694_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBK5051075.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            81990..82280
                     /gene="rplW"
                     /locus_tag="LK694_000173"
     CDS             81990..82280
                     /gene="rplW"
                     /locus_tag="LK694_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBK5051076.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            82319..83152
                     /gene="rplB"
                     /locus_tag="LK694_000174"
     CDS             82319..83152
                     /gene="rplB"
                     /locus_tag="LK694_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBK5051077.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            83195..83473
                     /gene="rpsS"
                     /locus_tag="LK694_000175"
     CDS             83195..83473
                     /gene="rpsS"
                     /locus_tag="LK694_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBK5051078.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            83496..83843
                     /gene="rplV"
                     /locus_tag="LK694_000176"
     CDS             83496..83843
                     /gene="rplV"
                     /locus_tag="LK694_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBK5051079.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            83857..84513
                     /gene="rpsC"
                     /locus_tag="LK694_000177"
     CDS             83857..84513
                     /gene="rpsC"
                     /locus_tag="LK694_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBK5051080.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            84516..84950
                     /gene="rplP"
                     /locus_tag="LK694_000178"
     CDS             84516..84950
                     /gene="rplP"
                     /locus_tag="LK694_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBK5051081.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            84940..85128
                     /gene="rpmC"
                     /locus_tag="LK694_000179"
     CDS             84940..85128
                     /gene="rpmC"
                     /locus_tag="LK694_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBK5051082.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            85153..85419
                     /gene="rpsQ"
                     /locus_tag="LK694_000180"
     CDS             85153..85419
                     /gene="rpsQ"
                     /locus_tag="LK694_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBK5051083.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            85477..85845
                     /gene="rplN"
                     /locus_tag="LK694_000181"
     CDS             85477..85845
                     /gene="rplN"
                     /locus_tag="LK694_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBK5051084.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            85882..86190
                     /gene="rplX"
                     /locus_tag="LK694_000182"
     CDS             85882..86190
                     /gene="rplX"
                     /locus_tag="LK694_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBK5051085.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            86218..86757
                     /gene="rplE"
                     /locus_tag="LK694_000183"
     CDS             86218..86757
                     /gene="rplE"
                     /locus_tag="LK694_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBK5051086.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            86776..86961
                     /locus_tag="LK694_000184"
     CDS             86776..86961
                     /locus_tag="LK694_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBK5051087.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            86997..87395
                     /gene="rpsH"
                     /locus_tag="LK694_000185"
     CDS             86997..87395
                     /gene="rpsH"
                     /locus_tag="LK694_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBK5051088.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            87427..87963
                     /gene="rplF"
                     /locus_tag="LK694_000186"
     CDS             87427..87963
                     /gene="rplF"
                     /locus_tag="LK694_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBK5051089.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            88124..88480
                     /gene="rplR"
                     /locus_tag="LK694_000187"
     CDS             88124..88480
                     /gene="rplR"
                     /locus_tag="LK694_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBK5051090.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            88501..89001
                     /gene="rpsE"
                     /locus_tag="LK694_000188"
     CDS             88501..89001
                     /gene="rpsE"
                     /locus_tag="LK694_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBK5051091.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            89016..89195
                     /gene="rpmD"
                     /locus_tag="LK694_000189"
     CDS             89016..89195
                     /gene="rpmD"
                     /locus_tag="LK694_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBK5051092.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            89239..89679
                     /gene="rplO"
                     /locus_tag="LK694_000190"
     CDS             89239..89679
                     /gene="rplO"
                     /locus_tag="LK694_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBK5051093.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            89679..90974
                     /gene="secY"
                     /locus_tag="LK694_000191"
     CDS             89679..90974
                     /gene="secY"
                     /locus_tag="LK694_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBK5051094.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            91036..91683
                     /locus_tag="LK694_000192"
     CDS             91036..91683
                     /locus_tag="LK694_000192"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBK5051095.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            91874..92092
                     /gene="infA"
                     /locus_tag="LK694_000193"
     CDS             91874..92092
                     /gene="infA"
                     /locus_tag="LK694_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBK5051096.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            92125..92241
                     /gene="rpmJ"
                     /locus_tag="LK694_000194"
     CDS             92125..92241
                     /gene="rpmJ"
                     /locus_tag="LK694_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBK5051097.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            92259..92624
                     /gene="rpsM"
                     /locus_tag="LK694_000195"
     CDS             92259..92624
                     /gene="rpsM"
                     /locus_tag="LK694_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBK5051098.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            92652..93041
                     /gene="rpsK"
                     /locus_tag="LK694_000196"
     CDS             92652..93041
                     /gene="rpsK"
                     /locus_tag="LK694_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBK5051099.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            93121..94059
                     /locus_tag="LK694_000197"
     CDS             93121..94059
                     /locus_tag="LK694_000197"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBK5051100.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            94087..94467
                     /gene="rplQ"
                     /locus_tag="LK694_000198"
     CDS             94087..94467
                     /gene="rplQ"
                     /locus_tag="LK694_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBK5051101.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            94739..95578
                     /locus_tag="LK694_000199"
     CDS             94739..95578
                     /locus_tag="LK694_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK5051102.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            95554..96423
                     /locus_tag="LK694_000200"
     CDS             95554..96423
                     /locus_tag="LK694_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK5051103.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            96413..97210
                     /locus_tag="LK694_000201"
     CDS             96413..97210
                     /locus_tag="LK694_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBK5051104.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            97413..98159
                     /gene="truA"
                     /locus_tag="LK694_000202"
     CDS             97413..98159
                     /gene="truA"
                     /locus_tag="LK694_000202"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBK5051105.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT        30840 a        18211 c        20486 g        29199 t
ORIGIN      
        1 tctttttccg gtggagcaag ctccggtgga aaaagaagcg ttcgacttgc atgtattagg
       61 cacgccgcca gcgttcgtcc tgagccagga tcaaactctc ataaaaagtt cgaacaatct
      121 tgcgattgtt aagctcaatt tgtttgctag catattgctt gcttgttaaa aatgtttgtt
      181 gtcttgaatt gaatcaagac gccctacaca tttggtttgt cttactttgt tcagttttca
      241 aaggtctact tcatcaggag tgtttcccga ttcgtttttg gttgccgtag caacttttat
      301 atcatatcag agagacaagt aattgtcaac tcttttttaa aagttttttc gatttcgttt
      361 ttcgaaatct tccagcaact catttatcat aacttatcgt ttgttatttg tcaagaactt
      421 ttttgaagtt tttttcgata gaatcaagct tcgcaagtgg ttctttttta acgactcagt
      481 tatcttatca tgtcatttcc aacatgtcaa caacttattt tttattattt aataagttat
      541 tggtgttctt ttgaacaaat aataatatat caacatcaca tgtgaaagtc aataagaaaa
      601 tgcttattta tcaacatttc tagcttacat gatcattggt tagttctggt cctttttcgt
      661 tcgtctttat aactccgaga gacgtaaaat ccttttgttt tcagtttaat gttcatgttc
      721 ttgtcttatt cacaccaact agatgtctca ccttctaaaa ttttccttaa caaagtgctg
      781 cttttatcta tttctgcttt tttttgctag aataatagta acaatatgac tctggaggat
      841 ttatttatga aaattttgat tgctgacgat gataaagaaa tcgttgaatt attaagtatc
      901 tatgttcaca atgaggggta tgaagcagta aaggcttatg atgggaaaga agcattatct
      961 aaaatcagaa caatccctga tattgaatta cttattttag atatcatgat gcctgagatg
     1021 gatgggatgc aagtagtgaa agagctacga aaagaatccc aaataccaat cattatgctg
     1081 acagcaaaaa ctactgatat ggataagatc aaaggactgg tcgctggtgc agatgattat
     1141 gtgaccaagc catttaatcc gttagaagta atggctagag taaagtcttt attacggcgt
     1201 acaaaaatgc agattgcccc agatcagcct gatgtattag aagtaaatgc attaatcatc
     1261 aataaagact ctcatgaagt taaaacaatc gatgggaaag aaatccagtt aacagctttg
     1321 gaattcggta tcctctactt gcttgctagt catcctaatc gagtatttag cgcggatgaa
     1381 atttttgagc gtgtttggaa acaagaaagt attgtctctg ctaaaactgt catggttcac
     1441 gttagtcatt taagagataa gattgaagaa gcaactggtg gtgaaaaagt cattcaaacc
     1501 gtttggggag tgggctataa aattgacgca agatagtaaa cgagcaactg aaaaacgccg
     1561 tcggatcaat ttgacttcaa aagaaatcag cgaattgtta gcggaaggaa tcatcacgat
     1621 tatccttttg cttctattaa atgtttctat tctggttgtt atcagttcgg tgatcaacag
     1681 ttcaccttct ttgacgaatg cgatttggga ttcaaagaat atttttgcag aacgattgaa
     1741 cactgatttg ttctggaacg gccggaattt tattattccc tttttctttc tattagatat
     1801 tggtgttttg tattggcgct tgattcgaag atatcgccaa atgcaattgc ggcatatcat
     1861 cagcgaactt cactacatcg caaacggaaa ttatgatcat cgtatccctt ttgaactgag
     1921 tggtgactta agccgagtgg taacaagtat caatggattg gtggatagta ccgtagcagc
     1981 gattgaagac gaacgtaaaa tcgaaaaatc aaaagatgaa ttgatcacga atgtcagtca
     2041 tgatatccgt accccactca cgtccatcat tggataccta ggactgatag aagatggcca
     2101 gtatcactca gaagaagatc ttttaaagta tacgcatact gcttatatca aagcaaagca
     2161 gatgaaatct ttagtggacg acttatttga atacactaag gtacgccagc ctgctgtccc
     2221 ggtgaatttt tctgcctttg acatgatcca gttgatcgaa cagttagcag ctgattttga
     2281 gttggaagct tctaaaaaaa atatccaaat acttgttcaa tcaaaagtag actcccttat
     2341 catggatggc gataccgaaa aacttgtacg tgtcttcaat aatcttttga cgaatgcgtt
     2401 gaaatacgga aaaggagcaa cgaagatcgt aatcgaagta gaacgtatcg gttcagaagt
     2461 tgttgcaaca gtcaaaaata acggagcaat gatcccacaa caggcaatcg ataatctatt
     2521 cgaccgtttt tatcgtgtag aagaatctcg ttcacaagca actggaggaa ctggacttgg
     2581 tttagcaatc gctcaaagca ttgtggcttt gcacggcgga tacatttatg caaaatcaga
     2641 taagcaatgg acttctttca tcatgcacct tccaatcaag aaaaatgaaa aattacctat
     2701 tgatacacag gaagttattg acgaaagctg aaatattcgc tattcttatt acaattatta
     2761 gaataaatga aaaacacaga acccagtaca ctttattctt tttcagagag ctgatggcag
     2821 gtgtaaatca gcaaaggata atttggaagt tccagttcgt ttacgtgccc tttcgaaata
     2881 agaaaggtcg gttcaacagc cgttatgtgt tcaacaagtg cagctgacgc tgaacttggg
     2941 tggtaccgcg aatttcagac tgattcgtcc caagaataat gggatggatc agtctttttt
     3001 tattttcaaa aaggaggaaa tcatatgtta gatgtaaaaa tgatgcgaca aaatattgaa
     3061 gaagtacaag aaaaattgat gactcggggc gtaaaaaaag aaacattagc tgaattcatg
     3121 gaactagatg aaaatcgtcg taagctacta gtcaaaagtg aagaattaaa aaaatatcga
     3181 aatgatgttt ctgctgagat cgcccaattg aagcgtgcaa aagaagatgc ttctgctaaa
     3241 atcgctgaaa tgaaagaagt cggtggcaac atcaaagcat tggatactga aattgctgaa
     3301 gtggatgaaa aattgaaaca aattgcaact actttgccaa accttcccca tgattctgtg
     3361 ccagttggaa cagatgaaga ggacaatgta gaaatccgtc gttggagcga accacgtaca
     3421 tttgcttttg aaccaaaacc acactgggaa attgcagaaa atcttgatat tttagatttt
     3481 gaacgtggcg caaaagtagc cggtagccgt tttgtctatt acaaaggatt aggtgctcga
     3541 ttagaacgtg ctgtctacaa cttcatgctt gatcagcata tttacgaaca cggctatact
     3601 gaaatgatca ctccttatat cgtcaacagt aaagcgatgt ttggtacggg acaatttcca
     3661 aaattcaaag aagatgtttt ccaattggag aatactgatc taacattaat cccaacagct
     3721 gaagtaccat taactaacta ttataatgat gaaattttag atggtgcaga tttacctatt
     3781 tatttcactg cactaagccc tgcttttcgt tctgaagccg gaagtgctgg tcgggataca
     3841 cgaggactga ttcgtcttca ccaattcaat aaagtagaaa tggtcaaatt ttcggatgta
     3901 gagcattctt acgaagaatt ggaaaaaatg acagctgatg cagaagatat cttgaaaaaa
     3961 ttgcatttgc cttatcgtgt gatggcctta tcaactggag acatgggctt ttctgcagct
     4021 aaaacttatg acttagaagt atggatacct gcacaagata cttatcgtga aatcagttct
     4081 tgttctaact gtgaagattt ccaagcacgc cgcgcaatga tccgttatcg cgataatgat
     4141 ggaaaaatcc actatactca tactctgaat ggttcaggac ttgcagttgg ccgtacagta
     4201 gctgccattt tggaaaacta ccaaaatgaa gatggttctg tcacagttcc agaagcgctc
     4261 gttccttata tggggaatct gaaagttatt aaataaagag gctgtgaaaa agccgcttag
     4321 tttcgagtat taagaaaaat tttgtgaaaa tggctttcca catttttgca taaattggac
     4381 ttaataccga gaaactggct tttgaacacc atttatcgag aggttgtgaa atcagcgaat
     4441 taacctgagt attagagaaa aaaatggaaa aatagtccct catattttac cgtttgttga
     4501 actaataccg agggtcagct gattgaacac cgtttagtgg agaggtcgta agcctacccc
     4561 ccagctccta gttacaagga caaattcaaa aaacagctct tcatatttta tgaatgttgt
     4621 acttgtgatt cggaggtggc aagggaacac tgtttattca aaaagaggct gggacagaag
     4681 cgtttaactc caagaaataa gaagaaattc acgaaaattg cttttcaaat ttttgtgaat
     4741 ttcttcttat ttccgaagga gttgcttcta cttccgccgt ttatacgtgt ttagagcgtg
     4801 agacaaaagt gttctttact tttgtcccac gctctttaat gtttttaaga ttcaactgaa
     4861 tagtttggtg cttcttttgt gatttgtaca tcatgagggt gtgattcttt taatccactg
     4921 ccgctcattt ggataaattg cgcttcatca cgtaattgct gtagattggc tgccccaaca
     4981 taacccattc ctgaacgcaa accgccgatc atttggaaaa taatatctgc aacacttcct
     5041 ttataagcaa cccgaccttc gattccttct ggtactaatt tatttgcttc attgacacta
     5101 ccttggaagt aacggtcttt tgaacctttt tccatcgcgc ctaatgagcc catacctcgg
     5161 tatgttttga atcgacgtcc ttggaagatt tcgaattcac ctggtgattc atctgtccct
     5221 gccaacatac ttcctagcat cactgcatgt ccgccggcag ctaatgcttt gacgatgtca
     5281 ccagaatatt tgatccctcc gtctgcaata atcgctttgc catattgacg agcaactgat
     5341 gctgcatcat aaatagctgt gagctgaggt acacctacac cggctaccac acgtgtcgta
     5401 caaatcgatc ctggtccgat accaactttt acgacatcta cacctgcatc atataaagct
     5461 tttgtcgctt cagccgtcgc aacgtttcct gcaatcaatg ttgcatctgc aaaagttgaa
     5521 cggatttctt gaatttttcg gatcacacct gcactatgtc catgagctgt atcgatgatg
     5581 attgcatctg cccctgcttc taataaagca ttcgcacgct caaatgtatc gcttgttaca
     5641 ccaacggctg ctgctacaag caatcgacca tgttcatctt ttgctgcatt tggaaattcg
     5701 atcactttct cgatgtcttt gatcgtgatc aatccgctta aacgaccttc gttatctacg
     5761 attggtaatt tttcgatttt atgtttttgc aaaatctttt ctgcatcttt tagagaggta
     5821 cctactgggg cagtcactaa gttgtctttt gtcatcacat cagcaatcgg catgtgataa
     5881 tctgttacaa aacgcatgtc gcggtttgta attatcccta ccaattttct gttttcaagt
     5941 gtttcaacga ttggcacacc actgatacga tatttgctca tcaattcttc tgcgtctgct
     6001 acaagatgct gtggcgttaa gaagaacgga tcaatgatta cgccactttc agaacgtttg
     6061 acttttctta cttcatccgc ttgctggctg atcgtcatgt ttttatggat cacacctagt
     6121 ccgccttgtc ttgccatagc aatggccatc ttgctgtcgg taacagtatc catacttgca
     6181 ctgatgattg gaatattcaa cttaatattt ttagctaatt gtacgctcat atccacttcg
     6241 tttggtaaca cgtgactttc tgctggtatc aataagacat catcaaaagt aaatcctttt
     6301 ttcgtgaact ttgtttccca gttggacatt tttagtacca ctcctctaat ttagtttatc
     6361 tattaaaata attgattttt tcatttaagt caatgatcta cttggaatca agttagcttt
     6421 ctcatagaat accaatataa agcgtttaca tctgtgtgta ttaaaagaga ttgtaaaaaa
     6481 gctgtcctgc atcaagtaat aagaacagcc aatcaaaaac agcttctcat gtttttgaat
     6541 tggttgggtt tattaccgca gatgcaaact tttgaacacc gtttagtgga gaggctgtga
     6601 aaaagctgct tagtttcgag tagtaagaaa aattttgtga aaatggcttt ccacattttt
     6661 gcataaattg gacttaatac cgagaaactg gcttttgaac accgtttatc gtaaaaaggg
     6721 actgtgacaa gacttttgtc acagtccctt actattattt aactatggat aaacaaaatg
     6781 aggttcttta ctacccctag atatctagca aaatagctga tctttttgct agtttatctt
     6841 cttgggacgg ataaacttcc aataatcccg tattttttct tcaagtagta acggattgct
     6901 aaagcagttg ccccgataat gataactact actggatcta atactggatt gataactggt
     6961 gtcaatagtg ttgtcgcaga aaacagtacg acccaaagaa gtgtcgttcc acctaggata
     7021 agcaatgtct tacccattcc tggtttcttg cttcgatctg ctccgggata ttcatattga
     7081 tagatgtatt tgtacatcat ataaaagacc cagccaccga ttgcagaacc tagaacgagt
     7141 gtagaaaagc cataagctgg cgttgttcca cgtgaaaaca atccggtaat cgcaatcatg
     7201 atacttaata gaccaagcaa cagcaacgta ttatctaacc acatcaataa aggggtggat
     7261 gaatttttta cttctgctgg cttattgatg attgcttccg ttctttcaga gactgtccca
     7321 aacaattgac gggctgttct tccgcctttt tgttctgcaa ctaaagtagg cagaatgtca
     7381 tgaagggctt ttactttttc ttcttcagac aaattcgctg cttctaaaga ttttttcaaa
     7441 tcaaaaatgt actgttgatt tcttttggtt aattttgttt ctaaactatt gttttctttt
     7501 acgattgctc gtaattgttc tggttccatt atctaatagt cctccttagc cgttcctttt
     7561 ctcaacggtc gtcacaaaag acattctacc atattctaca gctagtttga tagccctttt
     7621 acacattaaa gcggaataac attacgtcgc cgtcttgtac gacataatct tttccttcta
     7681 atcgaacacg tccagcttct tttgctgcat gcatgtttcc gtaatgatcc aagtcttcaa
     7741 aagagacagt ttctgcacgg ataaatccgc gttcgaaatc tgaatggatg attcctgctg
     7801 cttgtggtgc tttgattcct tttttgaatg tccaagcacg tacttcttgt tccccagcag
     7861 taaagtaagt agctaagcct aacaaatcat aagcagcacg gatcaactga tccaaacctg
     7921 attcttcgat tcctaaagct tctaaaaatt ccgctttgtc ttcttcatct aattctgcga
     7981 tttcttcttc tgcacgagca ctgaccacga tcacttctgc attttcttct gcagcaaagt
     8041 tgcgcacttc ttgtacataa tgattcgcgt ctgcatctgc tacgtcatct tctgagacat
     8101 tggctacata aagaattggt tttgtcgtca ataaaaatag tgatttaacg attttttgtt
     8161 cttcttcagt aaattcgatc gttcgggcag attttccttc ttccagtaca ggtttgattt
     8221 tatccagtac agctagttct gccactgcat ctttatcttt ggttttagca acttttgcta
     8281 cacgagtata gcgtttgttg atagattcta agtctgctaa cactagttcc aagttgatcg
     8341 tatcaatatc ttctagtgga tcaacacgtc cttctacgtg agtaatatta tcatcatcaa
     8401 aacagcgaac aacgtgacaa atagcgtcta cttggcggat atggcttaag aattgatttc
     8461 ccaaaccttc tcctttactt gcgcctttta cgattccagc aatatctgta aattcaaaag
     8521 tcgtcggaac agtttttttc ggatgtacaa gttctgttaa gcgttgcaaa cgccagtcag
     8581 gtacttctac catccctacg ttggggtcga tcgtcgcaaa gggatagttt gctgcttctg
     8641 ctcctgcttt tgtgatcgca ttaaacaaag ttgattttcc tacgttgggc aacccaacaa
     8701 ttccagcagt caatgccata aatttattca ctctttctct gcattttttt caattacttt
     8761 ttttaatttc ttttcaaaat ctcgacgggt catcatgacg atatgtccgc aattcataca
     8821 tctgattttg atatctgcgc ccattcgaat gacttcccag cgattcgtct ggcaagcgtg
     8881 aggttttttc atttcaacga tatcgtgcaa gtcgtacata aaaatagcct cctttttgtt
     8941 tattcttcat caaattgaat attcaagata tcaagaattc gagtcaaatc tttttgagaa
     9001 agatattcaa tctcgatctt acctttccca tttttttctt ggatagctac acttgtacca
     9061 aatttatcca tcaaacgatc ttcgctttct cggatataat atggtttttc ctttgctagc
     9121 cttgggattt ttttcttttc ttttccatca ttttgattta actcagaaac aagctgttcc
     9181 agttggcgga cagttaaatt ttcagttact gcacgattgg ctaatttcaa gatcaatgtt
     9241 ttatttttta aacctaacag cgtacgggct tgtcccatag ataaacgctg atcttgtacc
     9301 atttccttga caagatccgg taaagaaagc aagcgcaaat aattggcgat ataaggtctg
     9361 ctctttccta aacgactagc tacttctgct tgtgttagtt tcaggttttt catcagcatc
     9421 tcatatgctt ctgcttcttc taacggattc agatcttctc gctgcaagtt ttccagcact
     9481 gctacttgca tcatcgcttc ttcgtcaaag tctctaacga tagcagggat cgtttctttt
     9541 tcagctagtt tggacgctcg aaaacgtcgc tcccctgcaa taatctcata tccttttact
     9601 gcggaacggc gtacgatgat gggttgaaag acacctgatt gttcaataga attagctagt
     9661 tcctgcaaag atacttcttc aaatgtttta cggggctgat acggatttgg tcgcagctca
     9721 ttcaatggta gctggacaac ggtatcatct tttacatcca ccgtctctaa gttttctaaa
     9781 tcttgaaaca gtgcatcgat tcctcggcct aatcctttac ttttcttcac gacttaccac
     9841 ttcctttgcc aatgcttggt agacttcagc tcctcgagaa cgtggatcgt agtcgatgat
     9901 cggttttcca tgactaggtg cttctgataa acgaatattt cttggaatga tcgtttcgta
     9961 aactttttcg cggaagtatt tacgtacttc ttctacgact tcatttccca gatttgttcg
    10021 tgcatcatac attgtcagta agactccctc gatctctaat tctggattaa agtgtttttg
    10081 gactaaacga accgtattca gtaattggct taatccttcc aatgcgtaat actcacactg
    10141 aacaggtatc aaaatcgaat cacttgctgt aaatgaattg attgtcagat ggccaagaga
    10201 aggcgggcaa tcgatcaaaa tataatcata ctgactgctt acttctgcta aagatccttt
    10261 taatcttgat tctcttgcca tcattgacgt caattcgatc tctgctcctg ctaattggag
    10321 agtcgcaggc acaatactca aattttcgtg ttcagtgatc aaagttgcct catcaattgg
    10381 caattcattg actagaacat catagatgtc tcttgtcaca tcaggttttc tgatcccgac
    10441 accactggta gcatttcctt gtgcatccat atccactagc aatacttttt tgccaaggct
    10501 agcaagacaa gcacctagat tcaccgtcgt cgttgttttg ccgacgccac ctttttggtt
    10561 cgcaacagat attatttgtg ccatctcttc tcctcctatt tgattggctg cttattaggc
    10621 agtccgggct ttcttgggta tttctttggg gttggtttct ttttttgaat aacgatgata
    10681 tgccgttgat cagagacatt cggcaaggta accgcctctt ctttgaggaa tttaccacct
    10741 aataaagcaa tcgcttgttt tgcttctgtc aattcctctt cactttttgc tgctttcaat
    10801 gcataaaaat agccttcttg tttagccaag ggcaaacaca attccgctaa cacattcaaa
    10861 cgagcaaccg cacgagcagt gacaaggtca aatgatgcac gaaagtgagg attttgccca
    10921 aatgtttctg cccggtcatg atataaggaa gcatcgattc ctaataatcg ggttaactca
    10981 gataagaagg ttattcgttt attcagagaa tcaacgatcg ttatctgcag ttctggaaag
    11041 gcaattttta aaggtatgct aggaaaccct gcccccgaac caacatcgca taaagataaa
    11101 ggttgcgtaa agtctacttc aaatgctaat gtaagtgaat cgtaaaaatg cttcagatat
    11161 acgtcttttt catccgtaat agctgtaagg ttcatctttt cgttccattc gactaatgtc
    11221 cgataataaa tagcgaattg attcatttga tgatcggtaa gctctatccc tttttctgcc
    11281 aaggattggc gaaattcttc tggtatcata aatattcctt tcattgtgaa acagcaattg
    11341 tttcacagat tctctcccta ctttaacgca aaatctctag acatgcaatc cttccatatg
    11401 gattcttaaa aatttgaaaa gtaagaaaag ccgatagact ttttctttct agtatgactc
    11461 ataaaatata cacagaagag agaactagct ttgagcaatt tctgcaaaaa ctagttctct
    11521 cttctattta ttattacgta ttacgctggt tcactgactt tcgcaatctt cccttgttcg
    11581 atatagacca ttaggatact taggtctgca gggtttacgc cactgattcg gctagcttgg
    11641 gcaattgttt ctggttggat tttctgcaat ttttgttttg cttctgttgc taatccgttg
    11701 attgctgcgt agtcgatgtt ttctggaatc tttttcgctt ccatgcgttt taatttttct
    11761 actttttcca tggctttttt gatgtagcct tcatatttga tttggacttc tacttgttcg
    11821 atttcttttc gtgtcaattc ttcattttct ggaataaacc gtagaagttc tgcataagta
    11881 atctcaggac gtcttaaaaa gtcgcttgct agtacaccat cttttaatgg tgccgcatta
    11941 atcgttgcta aaaagtcttg gacttcttct gttggtttga ttcgtacatt catcaaacgt
    12001 ttgatttctt gttcaactgc ttcttttttc ttcaaataat tcttgtattg atcttctttt
    12061 actaagccta cacggtgacc catttctgtt aatcgcaaat ctgcattatc atgacgtaaa
    12121 agcagacggt attcagcacg tgaagtcagc aaacgataag gttcgttcgt tccttttgtt
    12181 accaaatcat cgatcatgac accgatgtaa ccgtcacttc gtttgatcac taaaggttct
    12241 tttccctgaa ctttcaaagc tgcattgata ccggcaatca gcccttgtcc agccgcttct
    12301 tcataaccgc ttgtaccatt tgtttgtcca gctgtataaa gattttcaac gactttagtt
    12361 tctaatgtcg gacggagctg gtgtggctct actacgtcat attcgatagc atatcctgta
    12421 cgcatcattt ctactttttc taatccagca atagaatgaa gcatgttgac ttgaacatcc
    12481 tctggtaaag aagtagataa accttgaaca tagacttctt ccgtatttaa gccttctggt
    12541 tccaaaaata attgatgacg tggtttgtct gcaaaacgca cgatcttatc ttcgatggat
    12601 ggacaatagc gagcgcctac cccttcaacg atccctgtaa acattggggc acgatgtaag
    12661 ttgtctcgaa taatttggtg agtcgtctca cctgtatagg tcagccagca agattcctga
    12721 tctagtttat aggcactatc tggtgtgctg aagctaaaat gattcggctc tttgtcacca
    12781 ggctgctctt ctgtaacaga atagtcgatc gtacttgatt ttactcgagg cggtgttcct
    12841 gttttgaacc gttcgatagc aaagccaagt tcttttagat gattcgctag tccaatagaa
    12901 ggttgagagt tattcggacc tgatgaatat ttcaattcac cgataatgat ttctccgcga
    12961 agtgctgttc cggctgtaat aacaactgct ttggcccggt agctcgctcc tgtggacgtc
    13021 actactccat gacaaacacc atcttcgacg atcagttctt cgacgattcc ttgtcttagc
    13081 gtcaaatttg gttctttttc gatcgtatgt ttcatttctg ttgaataagc atgtttgtct
    13141 gcttgtgctc gcaatgctcg gacagctggg ccttttcctg tgttcaacat acgcatttgg
    13201 atataggttt tatcgatatt cttacccatt tctccgccta acgcatcgat ttctcgaacg
    13261 acaacgcctt tagctggtcc gcctacagaa ggattacatg gcatgaatgc taccatgtcc
    13321 aaatttattg tcagtaataa tgtttttgat cccatacgtg cagcagcaag tgcagcttct
    13381 gaaccggcgt gtcctgcgcc aacaacgatc acgtcatacg catctgcttc ataatgttcc
    13441 aatcgtttgt tcctcctttt attttcctaa acaaaattga ctgaataatt gagtgattag
    13501 ctcatcttgt acactgtcgc cgacaacttc tcctagatag tcccagcaac gcgtcatatc
    13561 tatttgaacg agatccacag gcattcctga atcaatacct gcaatgactt cttcaagtga
    13621 gagggaagct ttttctaaca acgcgatatg gcgggtatta gaaacataag tagcgtctct
    13681 ttctcctgtt tctccgctga agaataaatc agaaatagct gattccaaac gatctagtcc
    13741 gtcgttttta gctacggaaa ttgaaaaaac tggttcattt tcgatcaatt tcttcaattt
    13801 ttcttgttcc agctgtgctg gcaaatctgt tttatttaga agaatgatcc gtttcaatcc
    13861 acttgtcgct tccaataatt gttcatcttc tgctgtcagt ggttcgcttt ggtttagaac
    13921 aagtaagatc aagtctgctt ctgctaatgc ttttcggctt ctttcgaccc cgatccgttc
    13981 caccacatct tctgtttccc gaattccagc tgtatcgatt agcttgagcg gtaccccccg
    14041 aacattgaca tactcttcga ttacatctcg agtcgttcct gcaatatccg taacgattgc
    14101 tttttcttct cttaacaaat ggtttaacaa acttgatttg ccgacatttg gccggccgat
    14161 gattgccgta cttaaccctt cccgcagaac cttcccttgc ttggatgtag caagtagcgc
    14221 ttggattctt tgttggatca tctgcgcttt ttcaagcaat aacttcgtag tcaattcttc
    14281 gacatcatca tattctggat aatcaatatt tacttctact tgtgctaatg tctctaaaat
    14341 ctcttgtctc aatgagcgga tcaaagcaga cagattgccg tctaactgat taagagctag
    14401 tcccattgct ttgtctgttt ttgcccgaat caaatccatc acagcttctg cctgtgaaag
    14461 gtcaacacga ccattcaaga atgctcgttt tgtgaattcg cctggctcag ctaatcttgc
    14521 accttctctt aacaatagct gcaaaatttg attgactaca acgatcccac cgtgacaatt
    14581 gatttctact acatcttccc gcgtaaatgt ctttggtgct ctcatcacgg agaccatgac
    14641 ttcatcgaca agctgattgc tcttgggatc aacgatatgt ccataatgga tcgtatgact
    14701 tggtacttct gataatcgtt tgtttccaga ttgataaact ttatctgcta gttgcacagc
    14761 ttgatcgcca cttaaacgaa cgatactgat tgcaccttct ccaggtgggg tagaaattgc
    14821 cgcaatcgta tcaaattcta aagtaatttc agccattttt tcacttcctt tttcattttg
    14881 cgcacaaaaa aagtgcccac tccacccttt atgcacaata aacggtgaac ttcgcacttt
    14941 gactgcttgt tcttgattaa attctggtca tagattatca cacttgctat aaggaatcaa
    15001 gtgtttaaaa caattagatg aagttgagcc tgttctgctt ccattacctt atatttaatt
    15061 gtccttggat gtctctttgc gttctttggt ttattcgaag agaagatact gtataaacaa
    15121 ggctttatct ttcttaaaaa aaacggtaca ctaaatggta gcgctatcta aactatacac
    15181 ttttgaaagg agaaaatatg tgagcccgtt cagatctgct gcattggaag aagaaagtga
    15241 ccctcggatc aacgatgtcg ttttatctac agggaatatc aaccagaaat atatcgtccg
    15301 cgatatcgtt tttgttgtgg aatgcttaga agctgatttg tttgacccat cttttcccaa
    15361 agatgacctt ttcttaacaa cgaagaggaa attaaagacg aaggtacttg aatatggtgc
    15421 gaatgcagta atcaactgtc attttgagta tcagcaaaaa aaacgcgacg gaaagtattt
    15481 tctggaactt tttgcttatg gaacagtgat ccaatttatc caaacaacga ttggttaaat
    15541 gaaacagaca aaaaaagcgg atcaaaactg aagacagttt cgatccgctt ttttctatca
    15601 aagttagtga ctacttcatc gtatactctg ataagaatga tttctagtaa aatatacttg
    15661 gtttgaaaaa ctttgtcaac gtgatttatt caaaaagtaa tggaatcaat aatatttttt
    15721 agcaggttct actactaaat aacgataagg ttcctcacct tccgaatgtg tttttacatg
    15781 ttcatctttg cttaatgcag catggatttg tttacgttca aatgcaggca ttggttctaa
    15841 gaaaacaggc tgccctgtcc gttttacttt ttcagccgtg cgatcagcta aacgctgaat
    15901 gatctcttgg cgcttttcac ggtaattgcc tacattgact actacagaca gcttatttct
    15961 tgctaaacga tggatgaaga cttgtgccag atattgcaat gcattcagtg tttttccatg
    16021 tttcccaatc agtatgcctt gcttttgtgt ttctaaatgg aaaacaacca tattatttga
    16081 ttctctttcc attttgacaa gagctggagc atcaagctgt tttgaaattt cagtaagata
    16141 aactgccaat tccgttaaag ctgcttcatc acctagctca cttacttctt ctgttttttc
    16201 ttcaagaatc gtttcgttga ctacctcgat ctcagctgtt ggttcttctt ccaaaatttc
    16261 ttctacagtc tgttctactg tttcagaaac tttttcgctg atacttggct ggatcgaaag
    16321 ccgtgcattc tttcgaccca ttcctaggaa accttttttt ccttcttcca gtatttcgat
    16381 ttcgacttgg tctttggcca aacccaaatc agccagacct agttggacag cttcgtctat
    16441 tgttgcagct tcataaatcg gcattagcat tacctcccct attatttccg ttttttctta
    16501 ggattcattg ctttcttaag agcacgttct ctttcgcgga ttctccgtgc ttcttcttca
    16561 cgctcttttc gaattttgaa agggttgttg atcagcagcg tttgtacaac ttggaaggca
    16621 ttggaaacta cccagtataa tgaaagaccg ctggctaaat tgacacccat cactaaaatc
    16681 attacaggca tcacgaaatt catgatcttc attgatgcat tcgattcaat ttgactcatg
    16741 ctagataaat acgtacttgc aaaagtaaag attgctgcta aaatcggcaa aataaatgtt
    16801 ggatccgcgt tccctaagtt caaccataag aaatgccctt gtttcaactc tggtacacgt
    16861 gaaatagatt gccataacgc catcaagatt ggcatttgta ctaataaagg aagacaacca
    16921 gcatatggat tgacgccatt ttctgcatat aaacgctgct gctcttctct taacttactt
    16981 tgtgtttccg gatctcttga actatattgt tgctgcagtg cttttaattt cggttgtaat
    17041 tcttgcgttt tacgcatgct ctttgtttgg aaatgcatca acggcaatag gatcacgcgg
    17101 atgatcagtg taaataaaat gatccctatc cctgcattac caaatgacaa cgccttgatc
    17161 gcctcagcaa agtaataaac aatataacga tcccaaattc ctgtactctg tgcgctgact
    17221 tccccagttc cgcaaccgga taagacgatg accaaactaa cgataccggc catcaaaagt
    17281 aaacgtttgt atttcttcac ttactctttt cctctctgtc gattattttt gcaagtttta
    17341 agacgtggac taaattggag cgaacctcat cataagtcaa ccccttcaca cttggtctag
    17401 caatcacaat aaaatccaac tccggatcaa tttcttcttt tatagaataa actgctgctc
    17461 ggatttttcg tttgacttca ttgcggttta ccgcgttgcc gacctttttt cctacagata
    17521 aaccaactcg aaaatgaggc tgtcccggtt tttccaaacg gtaaacgacg aatttgcggt
    17581 tagcaaaaga atctcccgta tggaacacgg cttgaaactc tcgttctttt tttactcgat
    17641 acgcttttct cattagtttt ctttcctttt ctatctctca cttacgctca taatcatacc
    17701 ataattacga ttgtcctttc atcatttccc taaaaaagag tataaaaaaa accactgaaa
    17761 ccccagtggc ctaagctgaa agaacttttc ttcctttgcg acgacggcta gctaacacac
    17821 gacgtccatt tttagtactc atacgtttac ggaatccgtg aactttttga cgtttacgtt
    17881 tatttggttg gtatgttctt ttcatatatt ccacctccaa aatgttctta tctattttat
    17941 aaacacagac atactttgat agtataacga caaagtagtg attttgtcaa tataaaatag
    18001 aagatcgatt gttaaaactt atccacaaga ttattttttt ctatgggaat cctatgtgga
    18061 aaaagttatg cacagggatt atccacagat aataaaaggc tggttttaca cagaaaacac
    18121 acgtgtaaaa aaagtaatcc acaatcaggc gatttcctgt gtattccgtc tcctaactat
    18181 tggtatatct gtatttttta tccacagacg caagaggaaa tctattggtt tttacgagtt
    18241 ttccacctgt gcattagtgg tgtggaaaac tttttttgac aactgtgttt ttctcttttt
    18301 tctttttgtc gattgtgtgg ataattgtaa ttaaaaaatt tttttcttcg gtttctgtgg
    18361 aaaactattg aaaaaaatgc taaattagta ctagttattc actttttaat gaaggaggag
    18421 gccttatatg gtatccctcg atgctttatg gaatgaatta aaagcaacat accaaaagga
    18481 tttatcgcct gctagctata atacatggat cgaaacagcg aaccctcgaa cccttgatca
    18541 aaaccagctc gttgttgagg ttcctagcaa gattcataaa gaatattggg aaaaaaacct
    18601 ggcgacaaaa atcgttgaag tcggttatat gttatcagga aatgagatta tccctcgctt
    18661 tattactggc gaagaagcag agcaagaaga agttatagaa gaaaaaaatc caaaagttgt
    18721 ggcacctagt ccgctaaaaa aagccatgct gaaccctaaa tatacctttg atacgtttgt
    18781 cattggtaaa ggaaaccaga tggctcatgc agcagcgctc gttgtagcag aagatccagg
    18841 ttctatttat aacccgctct tcttttatgg aggcgtggga cttggtaaga ctcacttgat
    18901 gcatgcaatt ggtcatcaaa tgctgcaaag tcagcctaat gccaaagtaa aatatgtaag
    18961 cagtgaaact tttgctaatg atttcatcaa ttcgatccaa aacaaaacag cagaagagtt
    19021 ccgtcaagaa tatcgaaatg ttgatttact gctagtggat gacattcaat tttttgcaga
    19081 aaaagaagct acgcaagaag agtttttcca tactttcaat gccttgtaca atgaagggaa
    19141 acaaatcgta ctaaccagtg atcggttgcc aaacgagatt ccgaaactgc aagaacgcct
    19201 tgtttctcgc tttgcatggg ggctgtctgt tgatattaca ccgccggatc ttgaaacaag
    19261 aacagcgatt ttacgtaaaa aagcggcggc tgaacgtcta gagatcccag atgacacact
    19321 aagctacatc gcaggacaaa ttgattctaa cattcgtgaa ttagaaggtg cgcttgttcg
    19381 agtgcaagct tttgctgcga tgaatagtga agatatttcg actagtctag ctgcagacgc
    19441 actgaaaacg ttaaaatcag gaaaaggaca tcctcaattg tcaatcctgc agatccaaga
    19501 agaagtggca aagtattatc acatccaatt gaaagatctc aaaggaaaga aacgcgtcaa
    19561 atctattgtt gttcctagac aaatcgctat gtatttggca cgtgagctca cagataattc
    19621 tttaccgaaa atcggagctg aattcggtgg caaggatcat acaacggtta tccatgctca
    19681 tgaaaaaatc cagcagctga tggattctag tgtcagtatg caaaatgaag tatcagaaat
    19741 caaaaacttt ttactaaatt aatttgtcga ttctatcaac tgaaaatagc atgtggataa
    19801 aagaaaaaaa cgttcaaaag ttatacacac tttttccaca gctgtttttt cgtttctctc
    19861 ttacgttttt tgagttttcc acagaatcaa catgccctat tacttttatt attcttttta
    19921 actaataata aataaacaaa cattcaaagg agatacatca tgaaagttac tttaaaccga
    19981 gctagcttta tgcaggaatt gcaaactgtt caacgagcta tttcaagcaa aaccacgatc
    20041 cctattttga caggtgtaaa aatcacactg acacaagaag gtttgacttt gacggggagc
    20101 aacgctgata tatcaattga aacttttttg tctgttgaaa acgaaaaagc aaatatgcaa
    20161 atcgaatcta ctggttccat tgttttacaa gcacgtttct ttagcgaaat cattcggaga
    20221 cttcctgaag aaacatttac tttagaagtt ttagaaaata aacaagtagc gatcacttct
    20281 ggaaaagcga attttatcgt aaatggatta gatgcagata actatcctca tcttcctgtt
    20341 gtcgaaagcc ataaccagat gaaattacct gtacacgtat tgactaaact aatcaacgaa
    20401 acagtttttg ctgtctcaca acatgagagt cgtccaatct tgacaggtgt gcatttcatt
    20461 ttatctgata attctttatt agctgtagct actgattctc accgtctaag tcaacgcgtg
    20521 attccagtag aacaagcggc tgatcatttt gatattgtta ttcctggaaa aagtttgatc
    20581 gaattatctc gctcattaac aaatgaagaa gaaatcgtcg aaatcagcat tatggaaaac
    20641 caagtgttgt tcaaaacaga aacgatgtat ttctattccc gtttgctaga aggaaactat
    20701 ccagatacca atcgtttgat tccttcaagt tttaatacag aggttgaatt ttctgttcct
    20761 agctttttag cggcgatcga acgggcttct ttactttctc atgaaggccg taacaacatc
    20821 gttcgtttat ctattcgtcc agatgcggtt gttttatacg gaaattcgcc agaaatcggg
    20881 aaagtagaag aaagcttgag ttatacagca agcagtggcg atccattaga tatttctttc
    20941 aatccggatt atatgaaagc agcgttgcgt gcatttggag atatgagcat caaagtgaaa
    21001 tttatctctg ctattcgtcc atttacattg gaaccaacgg aagatggcgt tcaatttatt
    21061 cagctgatta ctcctgtacg tacaaattaa caatgatttt caatgttctt gaaaatttaa
    21121 ttgaaaagaa atagaaaaaa taaaatttta aacagaaggc cctaaaatct cttttaaggc
    21181 tttctgttta tttttgttta aatcaccttt tttagattaa atcgtcttat atggaaaaat
    21241 atatggataa atttgtttta ttgcgaaaaa aaaggtataa tagagtatac ttttaataat
    21301 gaaaatgagg gatgggcatt gaaacaacaa gtcattctaa atacggaatt catgacgttg
    21361 ggccaaatgt tgaaagaagt cacagttatc ggtagtggcg gacaagcaaa atggtatttg
    21421 gcagaaaaca ctgtgttagt cgatggcgaa ccagaaaatc gtcgtggacg caaactttat
    21481 cccggcatga tggttgaagt tccagaagta ggaacttttt ttatggtaaa aaaaggaaac
    21541 gacgataatg aggctgaata agctgtattt aaaaaattat cgaaattacg aagagctgaa
    21601 catcgaattt tccaagaacc tcgtcatctt tttaggagaa aatgcgcaag gaaaaacgaa
    21661 tcttttggaa agtatctacg tattagctat gacgcgaagt catcggacaa gtagtgagca
    21721 ggaattgatt ggctggactg acgatcaggc gatgattcaa ggagagatca caaaaggttc
    21781 atccactctt ccattagaaa tcttattatc taaaaaaggc cgaaaaacca agatcaacca
    21841 tatcgaacag aaaaagttaa gtacttacgt gggacaaatg aatgtcattt tatttgcacc
    21901 agaagatctt tctttagtaa aaggcagccc gcagatcagg cggaagtttt tagatatgga
    21961 attaggccag atcaatccaa tctatcttta tgatcttgtc caataccaag caattttgaa
    22021 acaaagaaac cagtacttga agcaattgaa tgaaaaaaaa caaactgatt tgctttattt
    22081 ggatattttg tccgaacaac tagcagcgtt tggcagcaaa gtattgaaag caagagcaca
    22141 gttcgtcaag cgattggagt tttgggctaa ttctttgcat cagcaaatca cccatcaaaa
    22201 agagcagctg gaaatcgagt atctcaccgc agtcgattct ttagaaacac atacgcaaga
    22261 gcaaatccaa gaacaatttc tcgcactttt gaaccaaaat aaaaagaaag acctttttcg
    22321 gggaacaacg acagttggtc ctcatcgtga cgatctttcc ttttttatta atcagaaaaa
    22381 tgtgcagact tatggatcgc aaggacagca aagaacaact gcactaagtg tcaaattagc
    22441 agaaatcgat ttgattaaag aagagacagg ggaatatcca atccttttgt tagatgatgt
    22501 gatgagcgag ctggatgata atcgtcaact ccatctgcta gaaacaatcg aaggaaaagt
    22561 tcaaacgttc ttaacaacaa cgacacttga ccatgtaaaa aataaaatga ctgttgaacc
    22621 tgagattttt tatgttcaac aagggaaact aaaaggagaa gaaactgaat gacagaagaa
    22681 agaagtttag tagaacgcgc taaagagtat gatgccagtc agattcaagt acttgaagga
    22741 cttgaagccg ttcgtaaacg acctggcatg tatatcggtt cgactagttc tgagggtctg
    22801 catcaccttg tttgggaaat tgtcgataat tcgatcgatg aagtattagc aggttttgca
    22861 acgaaaatcc atgtgattat tgaaaaagac aacagtatca ccgttattga cgacggtcgg
    22921 gggatacctg tagatatcca ggcaaaaact ggacgtcctg cagttgaaac ggtctttact
    22981 gttttacatg ctggaggaaa attcggcggt ggtgggtaca aagtttctgg tggacttcac
    23041 ggagtaggat cttctgttgt aaacgcgctt tctacacagc ttgatgtcaa agtatacaaa
    23101 gaaggaaatg tgtactatca agaatatcgt cgtggagctg tagtggacga cttaaagatt
    23161 atcgaacaaa cggatcgcca tggaacaacg gttcacttta cgccagaccc tgaaattttt
    23221 acagagacaa ctgagtttga tttttcaaaa ttggctaccc ggatccgtga acttgctttc
    23281 ttgaaccgcg ggatgcgtat atcgattgaa gacaaacgag aagaagaacc agtcatcaat
    23341 gaataccatt atgacggcgg gatcaaaagt tatgtcgaat acttgaatgc aaacaaaacc
    23401 gtgatttttc cagaacctgt atatttggaa ggtgaacagc aagatatcgc agttgaagtc
    23461 tctatgcaat atacagacgg ctaccattcc aatatcatga gttttgccaa caatatccat
    23521 acgtatgaag gtggaacaca tgaatcagga ttcaagactg ctttaactcg tgtgataaat
    23581 gattatgccc gaaaacaaaa gctgatgaaa gaaaatgacg acaacttgac cggtgaagac
    23641 gtgcgagaag gattgacagc tgttatttct atcaagcatc ctgaccctca attcgaagga
    23701 caaacaaaaa caaaattggg caattccgaa gtacggacag tcactgatcg gttatttagc
    23761 gaacatttca tgaaattctt gctggaaaac ccaagtgtgg gccggcaaat tgttgaaaaa
    23821 ggattactag cttccagagc tcgtctagca gcgaaacgtg cgcgtgaagt aacgagacgt
    23881 aaaggtgctt tagagattag taatctgcca ggaaaattag cggactgctc tagcaacgat
    23941 cctgaaaaat gcgaattgtt catcgtcgaa ggagattcgg ccggcggatc agctaagcaa
    24001 ggtcgtaatc gagagtttca agccatcttg cctattcgtg ggaaaatctt gaatgttgaa
    24061 aaagcaagca tggacaagat cttggcaaat gaagagatcc ggtctttatt tactgctatg
    24121 ggtacagggt ttggagaaga ttttgatgta tcgaaagctc gctaccacaa attagttatc
    24181 atgactgatg ccgatgtcga cggcgcgcat atccgtacat tgctgctgac tcttttttat
    24241 cgttacatgc gtcctattgt tgaagctgga tacgtctata ttgctcagcc acctttatac
    24301 ggggtaaaac aagggaagaa tattacgtat gtacaaccag gaaaaaatgc agaagaagag
    24361 ttgaaacaag tcgttgcttc acttcctgcc tcaccaaaac caagcgttca gcgttataaa
    24421 ggacttggag aaatggatga tcatcaacta tgggaaacaa ctatggaccc aagcaatcgg
    24481 atgatggccc gcgtcagtgt ggacgatgct attgcagcag atcagatatt tgaaatgttg
    24541 atgggggatc gagtagaacc ccgtcgtgca ttcatcgagg aaaatgcaca ctatgtgaag
    24601 aacttagata tttaatcatg aaggaggaaa aagaatgagt gaagaaatca gagaaaacat
    24661 ccatgatgtc aatctaacca gcgaaatgaa agactccttc atcgattatg cgatgagtgt
    24721 tattgtcgca cgtgcacttc ctgatgtcag agatggtctg aaacccgttc atcgccgtat
    24781 tttatacggc atgaacgaat taggtgtaac accggataaa gcccataaaa aatctgccag
    24841 aatcgtcgga gatgtcatgg gtaaatacca tcctcatgga gaccgtgcga tctatgagtc
    24901 aatggtacgg atggcacagc cattcagcta ccgttatatg ctagtggatg gtcatggaaa
    24961 ctttggttct gttgacggtg acggtgccgc cgctatgcgt tataccgaag cacggatgag
    25021 caaaatcgct acagaaatgc tgcgtgatat caataagaac actgttgatt tccaaagcaa
    25081 ctatgatgat acagaaaaag aaccagttgt actacctgct cgcttcccga atcttttagt
    25141 gaacggaaca acggggatcg ccgtaggtat ggccacgaat atcccacctc ataacttgtc
    25201 tgaggtagta gcggctatcg atttgctgat ggataatcct gatgtgacga ccaatgaatt
    25261 gatggaagta ttacctggac cagattttcc aacaggtggt ttggttatgg ggaaatcagg
    25321 aatccgaaga gcgtatgaaa caggtaaggg ttctattacc gtacgtgcaa aagttgagat
    25381 tactgagatg ccaaatggta aagaacggat catcgtgaca gaattgcctt atatggtcaa
    25441 taaagcaaaa ttgatcgaac ggatttctga actgcacaga gacaagcgta tcgaagggat
    25501 cacagatttg cgggacgaat cttcccgtga aggtatgcgg atcgtcattg acatccgtcg
    25561 tgacgccagc gcatccgtta tcttgaataa cttatataaa ttgacatcct tgcaaaattc
    25621 atttggattc aatatgttgg cgatcgaaaa aggtgtgccg aaagttttga gcttgaaaca
    25681 aatccttgaa aactatatcg aacatcaacg cgaagtcatc acacgacgga cggtctttga
    25741 aaaagaaaaa gcagaagcaa gagcacacat cttagaaggt ttgcgtattg ctttggatca
    25801 tatcgatgaa atcattgcga ttatccgcgg gtctaaatca gatgacgagg caaaagcaac
    25861 aatgatcgaa cggttcgatt tatccgatcg ccagtctcaa gctattttag atatgcgttt
    25921 acgccgattg accggtttgg aacgggaaaa aatcgaaaat gaataccaag aactcttaaa
    25981 actgattgaa gatttaactg acattctggc aagaccagaa cgtgttacag aaatcatcaa
    26041 gaatgaatta gcagacttga atcaacgatt tggtgataaa cgacgcacag aattactagt
    26101 cggtgaggtc ttaagtctgg aagatgaaga tttaatcgaa gaagaagaaa tcgttattac
    26161 attgacaaac aatggttaca tcaaacgttt ggcaaataat gaattcagag ctcaacgaag
    26221 gggtggacgt ggtgtccaag gaatgggtat ccacaatgac gacttcgtca aaaatctggt
    26281 ttcttgttct acccatgata cattgttatt ctttacgaat aacggaaaag tgtatcgggc
    26341 aaaaggatac gaaattccag aatacgggcg tacagccaaa ggaatcccaa tcatcaattt
    26401 actaggtatc gattcgagtg aaagcatcca ggcaatcatt gcggtgacgg gagaagcaga
    26461 agaaggccac tatctcttct ttacgacgcg tcaaggtaca gtaaaacgta cttctgtcac
    26521 tgcttttgct aacatccgaa gtaatggtct gatcgcaatt ggtttgaaag aagacgatga
    26581 gctcgtcaat gtgctactga cagatggtca atctaatatc attattggaa cacataacgg
    26641 ctactctgtc actttcaaag aagaagcagt ccgcgatatg ggacgtacag cttccggggt
    26701 cagagggata cgcctgcgtg aggaagatta tgtcgttggg gcagccttaa tgaaggaaaa
    26761 tcaagaagta ttagtgatca cagaaaaagg ttacggaaaa cggacaaaag tatcggaata
    26821 tccagtgaaa ggccgcggag gtaaagggat caaaacggcg aacatcactg aaaaaaatgg
    26881 tccactagct ggattgacca ctgtttctgg agatgaagac atcatgctga ttacagataa
    26941 aggtgtcatt atccgtttca acgtatcgac tgtttctcaa actggacgtt caacattagg
    27001 cgtacgtttg atgaagatgg aagctgatac gaaagttgtc acgatggcag cagtcgaacc
    27061 agaagctgca gaacaaacag ttgaagaaca aggatcagaa gaataaccat caagactaag
    27121 tgtgtaagct agcaagaata accgattaaa aaatctggtt attcctgctg gcttttttga
    27181 ttagttgaat ttctcttaaa atattcgaaa ctgttcattc tgtattgctt ttgaccaaaa
    27241 tagagtgtat aattagagct tgtgagtagt ctaattaatt agataactct ccttgctctt
    27301 aggaacctaa gagccgaaag tccacaggga ggtgaatatc aatggaaaac acgaaatatg
    27361 aaatcatgta cattattcgt ccaaacattg atgaagaagc aaaaactgct ttaatcgaac
    27421 gtttcgatgc aatcttgaaa gataacggag cagaagttat cgaatcaaaa gattgggaaa
    27481 aacgccgttt cgcttatgaa atgaacggtt accgtgaagg tatctatcac atcgttaaag
    27541 tatcttctcc atcatcagca aatgctgtca atgaatttga ccgtcttgct aaaatcaacg
    27601 acgatatcat tcgtcacatg attgttaaag aagaagctta attgttgttt catgtgaaac
    27661 aatagaaaac gatgagagga gatgactatt ttgattaata atgttgtatt agttggacgt
    27721 ctgacgaaag atccagattt gcgttacaca gcaagtggta cggcagtcgc aacatttact
    27781 ttggctgtga atcgtaattt tacaaaccaa agcggcaatc gcgaggctga ttttatcaac
    27841 tgtgtgattt ggcgcaaatc agcagaaact ttagcaaact atgctcgtaa aggaacactt
    27901 ttaggtgtta ctggacgtat ccaaactcgt tcttatgata atcagcaagg acaacgtgta
    27961 tacgtgacag aagtagtagc tgacaacttc caattgctgg aaagtcgttc tgcatctgaa
    28021 aatcgccaac aaagcggcgg attccaaagt tctggtcaat cagctggcgg atttggcgga
    28081 aacaataact ctaatcaaac atctcaatca tccaacggta tgcctgattt cgaccgtgat
    28141 acttctgacc cattcggctc aagttcaacg atcgacattt cagacgatga tttaccattc
    28201 taaacgaatt tgataggagg gaaatagaat ggcacaacaa agaagaggcg gacgtaaacg
    28261 tcgtaaagta gactacattg ctgctaacca tattgaatat atcgattata aagatatcga
    28321 attactaaaa agatttattt cagaacgcgg taaaatcttg ccacgtcgtg taactggtac
    28381 aggcgctaaa aaccaacgta aaattactgt tgcaatcaaa cgcgctcgta tcatgggctt
    28441 gctacctttc gttagcgaag actaataaat cctaggaatt gaatgactga tccttttgat
    28501 cagctattca attccttttt ttctgtaaaa ataacctcta ttcgtttagt cgctcgaatg
    28561 acgaaagtta tggtaaaatc aaattagtca tatatgggag gaaaattatg gaaaataaaa
    28621 acaatcattt atccccgaaa cctctcttcc ttatcctgtt tatattaatc gaagtattgg
    28681 tagtcttaat tttaccattt cgttttgtta gcgcttcggt cattctattg atgaatttat
    28741 ggggtgttcg ctattggttg aaaatgaaac gttggcttat tttaaataaa cgtgaaaaaa
    28801 tccagcaggc ttcgactgta gctgaaacca atctagctta tgtctcagaa gtcatgcctg
    28861 taggagtgat tgcttatcag ccatcaagcc aaaaaatcga atggctcaat ccattagcat
    28921 tgaaaatcaa agatcaaagt ggattaacag aggaagaatt gctagatact ttttttactg
    28981 caaaagaaaa aaagaaaaac caagtctatc tagcagataa aacatttcag tttgatatgg
    29041 atacagtaaa gggtgttgtt tattttaatg atattaccca aaaccagttg ttaaaactcc
    29101 atcagacgaa cagtcagccg gtgattggta ttttatctat tgataactat gcagatgcca
    29161 ttgataaatt agatgacaaa gagatttctt atttgaactc tttcgtcact acactagttt
    29221 ctgactggat gaatgaatac catgtctttt tcaaaagaat caacgcagaa cggtttttct
    29281 tcgtagcaca gatgatcgat ctgaaaaaga tgatggataa aaaatttgat ttgctggatc
    29341 gctttcgagc agaagctgaa aaacagagct cgcctatcac ccttagtatg gggattgctt
    29401 atggctcaag aaccctcgat gaaatcggtg gagaagctca gaataattta gatgttgctt
    29461 tagtccgtgg aggcgaccaa gttgtcgtga aagaggtaaa agaaggagca aaacctgttt
    29521 attatggggg gaaatctgct aagaatgtca aacgcacgcg tgttcgttct cgagcgatga
    29581 gtaccgcttt gcgtcggatt tttgatgaaa caggcgatgt gtttatcatg gggcacaagt
    29641 ttccggatat ggatgcttta ggctctgctt ttggtgtcag ttgtctggca cgcttcaacc
    29701 agaaaaaagc ttacatcgtt atcaatgaga acgagatcat tccagatgta gaacggtgtc
    29761 ttgaagaaat ccataagcat cctgaattag aaatgcaact aatctctcca gaacgtgcca
    29821 tagagctgaa aaaagaagag gacctcctcg tgatggtgga ttaccataaa ccttctctat
    29881 cgatctctca gggcctatat gagcaatttg ataagattgt gatcattgat caccatcgtc
    29941 gtggggaaga gtttccaagt aaacctctgc tgacttatat tgaatcttca gcttcttcag
    30001 cttcagagct agtttctgag ctgatccaat accaaagcag caaaacaaat cgcttagatc
    30061 gttttgaggc cacattattg cttgcaggga tggttgtaga tacaaatagt ttcaatgtcc
    30121 gtacaactgc acgaactttt gacgttgcca gctatttaag gtcttgtgga gcagacgcat
    30181 ccttagtaca atatttattg agctcggatt tagcgacata cttggagatc agcaatttga
    30241 ttgctggaag cgactatgtg acttctgata ttgttgtggc ggcagggaca gacgaaaaag
    30301 agtataatag tgttatagca gcaaaaacag cagatacatt gctttcgatg atcaatatca
    30361 atgcagcttt cgtcattaca aagcggacag acggcttgat tgggatcagt gccagaagta
    30421 ccggcacgat caacgtccaa atcatcatgg aatccttagg tggcggagga cacttcacaa
    30481 acgcagctac tcaactggaa aatacgacag ttgaggaaac aaaacagaag ctatttgctg
    30541 caatcaaaga aaatatgaat caaatatatg ataaggagta atccaaatga aagtaatttt
    30601 tttacaagat gtaaaaggaa aaggtaaaaa aggggacatc aaagaagttc cagcaggtta
    30661 tgcacaaaat tatctaatca aaaataactt ggcaaaagaa gcaacgaaaa gcagtatcag
    30721 tcagttagca ggacaaaaga aagcacaaga aaaacacgaa gctgaaatct tggaagaagc
    30781 taaaaagtta aaagaattct tggaaaaaga agatactgta gtcgaattga aggcgaaagc
    30841 aggggaagac agccgtttgt ttggttcgat cccttcaaaa caaattgctg aagggttaaa
    30901 caaacaatac aaaatcaaat tagataaacg aaaaatcgaa ttagaaaatc cgatccgttc
    30961 actaggctac acaaacgtac ctgttaagct tcatcatgag gtgacggcta cgatcaaagt
    31021 tcatgtcgta caagaataac aaatcttgat agcgatgttc agctggtaag tataaccaga
    31081 aggaaaaaag acaaaccgaa atttatctga gaaaacagct gcagcaatga aagcaaaagc
    31141 tgcaaatcga tttcagattg atttcggttt cttttttggg aacagcaaaa tagcataaag
    31201 aaaacgagta taataaaaga gaactatttt tttattacac tttcggtacg aatgatgact
    31261 tttctctcat tttcttgtaa aatgaagcaa ttgaagacaa ggagcgatac gtatgaatga
    31321 agtgtggcag gatcgaatcc caccgcaaaa tattgaagct gaacaagcgg ttttaggctc
    31381 tgtttttctt gacgcagaaa cgatcattga tgccatggag tacatagagc caaaagactt
    31441 ttatcgtcgt ggccatcaaa ttatttttga gacaatgatc gagttaaatg atcgtaatga
    31501 agcgattgac gtggttactg tcaaagaccg gctggaacaa gccaatttgt tagaagatgc
    31561 cggtggttta agttatctct cagacttggc attagcagtt ccgacagcag caaatatcgt
    31621 ctactatgcg aaaatcgttg aagaaaaatc attgctgcgt acattgatcc agacagcaac
    31681 aggaatcgtt acgaaaggat tcgagcaagg agaagaagtt caaacaattt tagatgatgc
    31741 cgaacgcagt atcttggagg tttcggaaaa aagaaatcga agcggctttt tatctattgc
    31801 tgaagtactg aactccacga ttgaaaatat cgatcgacta gctcaaaaca atgaagaaat
    31861 cacaggtcta ccaactggct atcaaggtct ggataaaatg acagcaggtt tccagcccga
    31921 agaactaatc attcttgctg cacgtccagc ggtaggtaaa acagcgtttg cattgaatat
    31981 tgctcaaaat gtaggaacaa aaacagatcg ttctgttgcg atatttagtt tggaaatggg
    32041 ggcagaatct cttgtaaatc ggatgctttg tgcagaaggt tcgattgagg ccagccattt
    32101 acgtacaggt cagctttcgg aagaagaatg gtcgaatctg atcgttgcga tggggagtct
    32161 ttctcgagca aatattttta tcgatgatac accagggatc aaaatctctg aaatccgtgc
    32221 gaaatgtcgg aaactagcgc aagaaaaagg caatctaggc ttgatattga tcgattattt
    32281 gcagttgatt gaaggtaacg gtaaagagaa ccgtcaacaa gaagtatcag atatctctcg
    32341 ccaactgaaa aaactggcga aagagctgaa agtacctgtc attgctcttt cacagttatc
    32401 tcgtggagta gaacagcgac aagacaaacg accagtgcta agtgatattc gtgaatccgg
    32461 atcaattgaa caagatgcgg acatcgtagc cttcttgtat cgagatgatt attatgaaag
    32521 agaaggcgga gatgacgatg atggaaacga gccaaaaaat gacaacatca tcgaagtcat
    32581 catcgagaag aaccgaagcg gtgcacgagg aacagttgaa ctactgttta tcaaagaata
    32641 caataagttt tcgtcgcttt cgccaagaga agaattttaa agaagaggtt gtgagccttg
    32701 agcaatcccg agagctctcg taagaagatg caaggagtaa ccgcaggaac gactagagta
    32761 cttaatttaa acagatctcc aaagggattg tgacaaaagt cttgtcacag tccctttttc
    32821 cgaataaacg gtgttcaatc agctgaccct tggtattagc tcagcaaacg tcaaaatatg
    32881 aggaactatt ttgacgtttg tttctctaat actcaggtca aaacgctgat cttacgacct
    32941 cttttcctta aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatcatcaaa
    33001 catgagaagc agttttttga aattgctcct tgtgagtcag agttaaacgg caggctcaca
    33061 acctctttct taaacggtgt tcaaaagctt gcatctgcgg taatacgccc aatcaaacga
    33121 aaaatatgag aagctatttt tgtttggctg ttcttattac ttgatgcagg acagcttttt
    33181 cacaacctct gattcacggt gttccaaaag tagcttcttc gaaaaataag tcgaaatttc
    33241 acaaaaattt gagaagcaat tttcgtacat tccttcttat ttcttgaagc tgaccacttc
    33301 tgttacaacc tcttttttaa taaccattct aaaaagataa agttcgtttt ttaagcgttt
    33361 ttataaaata aaaataaaaa tcattcgatt ttatttgaaa tatgtatcta ttattgatac
    33421 aatggttcag gattaaataa tatagaacga ggtgttcaca tgtcatctgt agtagtagta
    33481 ggtacgcaat ggggcgatga agggaaagga aaaatcacag actttctcag tgaaaatgct
    33541 gaagtgattg cacggtacca aggtggcgac aatgcagggc acacaattaa gtttaacggt
    33601 gtgacttata aactgcattt gattccttct gggatttttt ataaagacaa gatcagtgtg
    33661 atcggaaacg gcgttgtagt caatcctaaa tcattggtta aagaattagc ttatttacat
    33721 gaaaaaggtg tagaaacaag taatttgcgt atttctgacc gcgcgcacgt aatcttgcct
    33781 tatcatatca aattggacca attacaagaa gatgctaagg gcgaaaataa gattggtaca
    33841 acgattaaag gaatcggacc tgcctatatg gataaagcag ctcgtgtagg tatccgtatt
    33901 gcagacttat tagataaaga aatttttgaa gaacgtcttc gtttgaatct agatgacaaa
    33961 aaccgtcaat ttgtaaaaat gtttgattca gaaccacttg attttgacga tatttttgaa
    34021 gaatattatg aatacggaca acaaatcaaa caatacgtca cagatacttc cgttatcttg
    34081 aatgatgctt tggatgcagg caaacgcgtg ttgtttgaag gcgcacaagg tgtaatgctg
    34141 gatatcgacc agggaactta tccttttgtt acttcttcta acccagtagc cggtggggta
    34201 acaatcggaa gcggtgtagg gccttcaaaa atcaataaag ttgtcggcgt ctgcaaagcc
    34261 tatacgtcac gtgtcggtga tggaccattt ccaactgaat tatttgatga aacaggagaa
    34321 caaatccgcg aagtaggacg agaatacggt acgacaactg gtcgtccgcg tcgtgtaggc
    34381 tggtttgatt ctgtagttat gcgccattct aaacgagttt caggtattac caacctatct
    34441 ttaaactcga tcgatgtatt aagtggattg gaaacagtaa aaatttgtac ggcttatgaa
    34501 ttagatggcg aactaatcta ccactatcca gcaagcttga aagagttgaa ccgctgcaaa
    34561 ccaatctatg aagaattgcc aggctggtca gaagacatta caggatgcaa aacacttgca
    34621 gaactgccag aaaacgcacg taactatgta cgtcgtatct cagagcttgt tggtgtacgt
    34681 atctcaacat tttctgtcgg acctgataga acacaaacaa acatcttaga aagcgtctgg
    34741 gcgcaaatct aaggataaaa acgacgactg aatcaggacg ttcttaccag agcgagtcga
    34801 agtgtttatc tatacgataa taataacttg aagaggtagt gaaagagacg tttagcctta
    34861 ttgccgatag gctgtctctt gaacactgtt tattcagaat aagggactgt gacaaaaacc
    34921 gtgtcacagt ccctttttcc gaacaaacgg tgttcaaaag cttgcatcta cggcactaag
    34981 cccaatcaga tgaaaaatat ggagtgctat ttttatctga ttgttcttac tgcttgatgc
    35041 agagcagctt tttcacaacc tctccactaa acggtgttct cctgccaact cctcggtcac
    35101 aagtcacaat attcaacaaa catgagaagc tgttttttga aattgttcct tgtgactcgg
    35161 agctaaacgg caggctcaca acctcttggt aaacggtgtt caatcagctg accctcggta
    35221 ttagctcaaa aaacggcaaa atatgaggaa ctattttttc cgtttttttc tctaatactc
    35281 aggtcaaaac gctgatttca caacctctga ttcacggtgt tccagaagta gcttctacgg
    35341 aaataagccg aaatttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc
    35401 ttgaagctga ccacttctgt cacaacctct tattttgatt agaacttgcg aaaataaacc
    35461 ggttaaagtg tgtctagcgc aagagatttg ctataataga cgataagggt aaaatgtaac
    35521 gagaaggggt tgaaaaatgt gtatcaaatt gtaacagatt cttgttgtga tctgccatac
    35581 catgtattag caaaagaaaa agttgatttt ttatcaatga atctccaatt gaatgatcaa
    35641 gagttagtag atgacctcgg aaagacattc aattatgacc gctacatgaa tcagctgaaa
    35701 aatggtgcga tgcctacgac atcacaaatc aatgttggcc gatatttaga atttttccgt
    35761 tcttatgcgg aaaaacagat tcctgtttta tatgtggctt tttccagtgg attaagcggc
    35821 tcttacagta gtgcgcttca agcggttgaa ttgctgaagg aagaatatga agaccctgag
    35881 atctatgttt ttgatacaaa agctgcaagt attggggaag ggcttttagt attggaagct
    35941 gtaagattga aaaaagaagg taaaagcatc gaagaaaccc tagagtggct ggaaagtaat
    36001 tatttgaaag tccattcatg ggtaacagta gacgatctga aatatctaga acgaggcggt
    36061 aggatttcca aagcaagtgc tactcttggc agtctattga gtgtaaagcc tattattacg
    36121 gtggataaag aaggaaggct gacgaatgtt gacaaagttc gaggaagaaa caagtcgatc
    36181 cagacaatcg tcaatgagac ggtaaaaaca atcgttgcac cacttgatca aatcgtttat
    36241 gtggcttacg ctggggatct agagagcgcg gagaaggcaa aacggctttt ggaagagcag
    36301 ataaagatga aagatgtgaa gctttatccc ttaggaccaa cgatcgccag tcacacaggc
    36361 tatggctgta tcgcgatttt ttcgatggga atcagtcgat aaaagcagta actcattctg
    36421 taaaaaagcc cagaaattga ttgtaacaaa aacagcgact cttaaaagat agatgagcat
    36481 cctatctctt agaggtcgct gtttttttat tttttaagct aaataggcca ttatgcccat
    36541 aactcgttca attgtttttg aatatctgca ttttccaaaa attcatcata tgtcgtatca
    36601 gctcgatcga tcacaccttt atccgaaacg gcaatgatgc ggtttgccaa tgtttggata
    36661 aattgatggt catgtgacgc aaataagatt gagccagtaa atgccatcaa gccgtcattc
    36721 aatgctgtga ttgattctaa gtctaaatgg ttcgttggat catctaatac aagtacgtta
    36781 gctttcgaaa gcatcatttt tgaaagcatg caacggactt tctctccacc agataaaaca
    36841 ttcactggtt tcaatacttc ttcaccagaa aacaacattc ttcctaagaa gcttcttaag
    36901 aaggtgttat catcttcttc tttacttgca tattgacgca accagtctaa aatcgttaga
    36961 tcagtatcga attctttcgt tgtatccttt ggtaaataag aattacttgt tgtcacaccc
    37021 caacgtactg ttccagtatc tggcgtaatt tcacccatga taactttgaa tagcgttgtt
    37081 gttgttatat cattttctgc gacgaaagcg accttgtctt ctttgctcaa attaaaagat
    37141 atatcatcta aaattttctt tccatcaatc gttacactta cattttctac ctgcaataag
    37201 tcgtttccaa tctcacgttc aggtttgaat ccaacgaatg gataacgtct agaagaaggt
    37261 tggatatcgt ctaaagtaat tttttcaagc atttttttac gtgaagttgc ttgttttgat
    37321 tttgatgcat tcgcactaaa gcgggcaata aattcttgga gttctttgat ttgttcttct
    37381 tttttggcat ttgcgtttgc ttgaagttta gccgctagtt gactagattc caaccaaaaa
    37441 tcataatttc ctacataaag tttgatttta ccaaaatcta gatccgccat atgtgtacat
    37501 actttgttca aaaagtgacg gtcatgggat acaacgatca cagtattttc aaaattgatc
    37561 aaaaattctt ccagccaatt gatcgattgg atatccagac cgtttgttgg ttcgtctagc
    37621 aaaagaacat caggtttacc aaatagtgtt tgtgctaata acacttttac cttttgtcca
    37681 ccagttaatt cactcatttg ctgatggtgc aattcttcag ggatgttcag accttgcagt
    37741 aaaacagctg cttctggttc agcttcccat ccattaagct cagcaaattc tccttctaat
    37801 tcagctgctc gaataccatc ttcgtcagtg aaatcctctt tcatgtaaat cgcatctttt
    37861 tctttcagca cttcatacag acgcttgtgt cccatgatga ctgtttcaag tacagtatag
    37921 tcttcatagt cgtaatggtt ctgtttcaat acggccatcc gttcatctgg tcccatagta
    37981 acggaacctg tggaaggctg cagatcccct gataacactt taaggaacgt tgatttacct
    38041 gcaccatttg caccaatcaa gccatagcag tttccggggg taaatttaat attcacatca
    38101 tcaaatagtt tgcggtctga aaagtgcaaa ctcacatcat ttacagtaat caattttttt
    38161 cctcactttt atcattatta gttttgacaa gtcttataga attatatagg ggaaaggtaa
    38221 aagattcaag gcaatcgaac attctttgtg tttcacgtga aacagaatga aaaaaataaa
    38281 aataaaagcg gagcgaaaat agctactata ggatttatta gtttgcaaca gcataatttt
    38341 tgacgttatt tataagaggg agtactataa aactgtaaaa gaaatgaaag aaggattgaa
    38401 aatggatgaa aaaagaacca tcgactgggg ctcattgatt ttgggaattt tatttgtact
    38461 tgtctcattg ttatcatttc aagaccctgt cggaaattta gttgcaattg tagtagtctt
    38521 tgcgattttt gcctttatta aagggatatt tgaactgttt gtacggaaca gattaaaaga
    38581 gctaacagga tataaaggaa agatgccctt aataatcggg atcatcgata ttctagttgg
    38641 cgtctttttc ttattcaata ttggtgcagg agtcgcagca ttgccttttg tttttgcagt
    38701 atggtttatt gctgattcag ttttagcgtt atttactgct gacttggcaa gaggagtaag
    38761 tgaaggatat tattggttta cgatcatcgt caatatttta ggaattttat taggaatttt
    38821 cttattgttt aatccaattt cttctgcgtt gacattaagc tttttagttg gattctattt
    38881 tatgttattt ggtatcacac atatcgtcta tgcatttaga taagtaaaat caaacaaata
    38941 aaaaagccgg cttccgatta cagttttact gttgtaattg gaagccggct tttttatcat
    39001 ggacaaatat gcacgaggtg atttttaggt gtcgagacaa atttatgcaa aaatgtgaga
    39061 agctatatac acaaatttta tcttaatact cgaagcatag cgcagttgtc acaacctctt
    39121 ttcatataat tcattcaata ctggcaaatc atggacttca tatttttctt tgaatgcttg
    39181 gatttcttct tcagaatatt ttcttggatc taaatgcaga tcttctactg gcaatgggcg
    39241 cttgttattg atcttcacat cgattactac aggacgatct gattttgcag ctgcatcaaa
    39301 ggcaaatcga agttgcgagt attctgtgac tgtaaagcta tttgccccga gcgcttctcc
    39361 aactttaccg aaatctgcat cttctaaaaa tacaccaaat ttcttttgtt ccgtatcttc
    39421 ttgttctgct tcgatgaaac caaaagaatc attcgaaaag acaacattaa taattggaag
    39481 ctgatatttg acttgtgtaa tgatatcttg catgaccata gcaaacccgc catcaccact
    39541 taaggtaaat acttgttttt ctgggtagct tagttgtgcg gcaattcctc ctggcacacc
    39601 attacccata gttgcgaacc atccagatgt cgtatgtttt tgtttaccgt tcatattcaa
    39661 taaacgaata gaatggatag tcgtgttacc gacatctgtg acgaaaatcg catcgtcttc
    39721 agcaatacga ttgatttctt tatagacagc ctctggacga atcggttctt cctctcgatc
    39781 ttcaaaagat ttcaaccaat ttacccattt ttctttattt tcttgatttg ctaggtacca
    39841 gctatcagca gggcgagcat cccctaactc gacaagtctt tgcaaagctg tgtttgcatc
    39901 accgaggatc gacaaagatg tttcatggcg tcgtccaaat tttgacgcat cgatatcgat
    39961 ttggataaat tcagcagttg gattaaaaaa tgcacgtcca aatgggaagt cgctaccgac
    40021 aaataagatc agatcggctt ctgccaatgc ttcatttccg ggtttagtag cgacacgtcc
    40081 agcaaaacca agaaagtttt cataatcatc aggaataatt ccttttgcta gaacggaagc
    40141 gacaacagga actgaaaaat gttctgagaa ttctttgatt tgcggaaatc cgtttcgcgt
    40201 tccttgacca acataaagaa ctggtttttt tgctttttca agataaggca aagcagctag
    40261 caaatctttt tcttctggta atatcacact tgttttgtga gtgtgagcgg tggagaaagg
    40321 ttgttcgtca atttcttcaa aaccaagatc aacagggata gtcacgatag ccacgccttt
    40381 atgctcatag gcggctttaa tggcttcatc tactacatgc ggaagacttt gtggtgtcat
    40441 gactgtacga ttgtatacgc taacatctgc gaaaatagga ttttcattta gttcctggaa
    40501 agagttatag ttcatggatg tagaagcaac ttgtcctaag aacgctacta gaggcacatg
    40561 gtccatctgt gcatcataca aaccattgat caaatgtgtc gcaccaggtc ctgcagagcc
    40621 gaagacagcg ccgactttcc ccgttaattt tgcatctgca gcggctgcga gtgcgccgac
    40681 ttcttcatga cgaacttgaa tgtagttgat ttcggttttt tcttgatata gcgcatccat
    40741 aattgaattg aaagatcctc cgggaatgcc gtaaatatgg tcgatacccc agctttcaag
    40801 aacttttacc attgcgattc ctgcattgat ttttgacatg gtgtttcact cctttagcaa
    40861 tgatgataca taaatttttt gtatctagag tatagaactt tttatcgaaa atcccaacta
    40921 attcgtcttt tcacaaaaat gtagtcatgt ttttgggaat atgctagagt agtacataga
    40981 tagattcaag agaggggaat ggcatggttg aatggctatt gagacttgaa ccttggcagc
    41041 aagcacttgc tgggacagct tttacttatt ttatgacagc tcttggtgcc gggttggttt
    41101 ttttctttaa agaaatcaaa aaagaagtgc taaacttgat gttaggattt gcttctggtg
    41161 tcatgattgc tgcaagtttt tggtctttgt tagatccggc tattacaaaa gcagaagaaa
    41221 atggagatat tgcttggcta gttgtaagta ttgggtttgg tttaggcggt gtgtttttat
    41281 atatggcaga taaaacactg ccccatatgc attttggacc acaacatgaa gcagaaggat
    41341 tgcctaccca tttaaaacgg acgatcttgc ttgttttttc tattacattg cataacattc
    41401 ctgaaggttt agccgtgggg gtagcattcg gtgcagcagc aactgcggat aatccgacag
    41461 ccgctatatt agccgctgtt tcggtggcgc taggtattgg tatccaaaac ttcccagaag
    41521 gagcagctgt atcaatcccg ttgcgacaag aaggtctgag tcgtaaaaag gcctttgttt
    41581 acggacaagc ttctggaatc gttgaaccaa ttgctggagt gattggagcg ctactagtaa
    41641 cgaaagtgga gctgctgctt ccttatgcac ttgcttttgc cgcaggagca atgatttatg
    41701 tcgtggtgga agaattgatc cctgaagcac agcagacatt gtccagcaaa cgtcattttg
    41761 cagtttttgg cgtcatgagt ggatttatca ttatgatgat tttggatgtg gcattaggat
    41821 aaataaaaaa aatatatatg atatatcaaa tagggccgcg aacttatatc gcgaccctat
    41881 ttttttagta agttatatca taatgaagtg aatggtatca tacattaata atagtttttt
    41941 ctatgtgtat ttcctttgat ccagcgaaca agccagatga taagtgcgat tgggattaga
    42001 actttgaccg caaaccataa aagcccgctt aggacactga caaaaagact gatgacgaag
    42061 ctagctgcaa ttagcactaa aagaaccatg aagatattta agaacatatt tttttgacgc
    42121 atacaaaacc ctcctcttgt tcattcttta ctatagaaaa agtataacag ggattgttta
    42181 aaaacacctc caactgaaga ctgattttta cttcatctac ttaaatgatc aaacaattgc
    42241 tttttctctg gtttatcatc taaaatagag cggaaaagga ggagaatatc atgcctaaaa
    42301 atcgtcttga agcatttaca gatgctgtaa tcgcgattat catgactatt ttagttttag
    42361 aacttcatga acctaacgga gacacgtggg aggcgctcgc tgcattaggt taccgatttt
    42421 ttatctatat tgtcagtttt atcattttag cgatttattg gaacaatcat catcatttat
    42481 ttcaagttgt acataagata aatggacggg tcttatgggc gaacagtttc tttgtttttg
    42541 cgctttcgct ttttccgttt gcaacttcat ggatcagtga gtacatcaat agcctggctc
    42601 cagaaatgac ttatggtttt gttaccttga ccgctaatat tgcgtattat atcttggcaa
    42661 aagaacttgt caaagcaaat aaaggccacg agaaagcatt gaaggcgatg ttgaataatt
    42721 acaagaaatc gtatctatcg attggtttga atgtgttagg aattgtctta gggtatttag
    42781 tagcgccagt agccgtgctg atcgcaaaca tcgtgattct agctgcttgg atcatcccaa
    42841 gtcgtaccat cgaatcacaa tatcggtaaa atgatccgtg tttaaaattt aagagaaaaa
    42901 tatagaaaaa ataaataaac gatcaaatat ccgagtgata ctagtattca aagagggatg
    42961 cagatggatc aaaaaagaat tcatgtagga ctgattacta gttttgtcac gatgcttatt
    43021 tatttggatc aaggtcattt gacttggaca gtagttggtg tcgtaggatt tttgacatta
    43081 ttcacagcaa taaaaaaata aacatcttta tgtaatctga tgttctatct atttaagaaa
    43141 agcatgttta cagcagtatg attctgttgt aaacgtgttt ttatttgtgg aaaatttaat
    43201 ttttagaaaa gtgtatcgaa aaatagatag ttagtttttc cacagtggat aacttcgtaa
    43261 gagataaaaa taaaaaatta gtcaaagaag ggcgtataga tttcttttga taggagatat
    43321 acaacagaaa tgaataaata ttaacgtgtt ttcaaacagt ataaaacggt gtggtttttg
    43381 gaataaaaaa ataacatatc tcattttaca cttttgttat tttatactta aaaaataacg
    43441 acacagaaaa taccgtgtct cagatttctc tactacttct aaaaactact gtttttttcc
    43501 tttgaaatgc cttcaaaaag taggatcagg cagaatatac tggcagaatc aaggagaagg
    43561 gagcctaaat aaggagaaaa accatagaaa atataaagta attacttata aaacaagctt
    43621 tttatagaaa aataatatac ctgagtaggg tataaattaa gacggaattc ctttctagat
    43681 tgagatattg actaggaaga atgttaagat tttaagcctt taagctaaaa aaaaaccctt
    43741 tttgttaaat aaggggaata tagcctgtca gtttcaaact gcaagtcgtc ctgctgcaat
    43801 aatatagtag attagagatg aaaatagatt tttttaaacc aatgactgaa taaaaagacc
    43861 gtcatagata cacattggaa tgtgtctatt acggtctttt tttacgaacg attttattga
    43921 agacggacag ttcgggtcaa aaataaaaaa gctacgatca gaaataagaa taaaatgtat
    43981 cccaaaataa tggctagacg gtctaactta tttgtcgata gatgtttgtt tagaagttga
    44041 taggcccata aacctatcag tcctcctgtt gtattagtta tcaaatcagt gatgtccgct
    44101 acgccaatac cgaataggaa ttgaatcaat tctgccaaga agcttacaag gaaaataatc
    44161 cctaattttt tagaaaaaga aagtctcttg aaattgacat tcaaaagcac cccaaaagga
    44221 aaaaagacaa tcaagttata agtggtctca cgtaataccg tcgtattttc aaaagaaaaa
    44281 gtagaaaaag ggacgagatt gacattgctg taagaataag tgagaaattg tggaagatta
    44341 aatgatagct tgaataaaat caaccataat agaaagaata aataaatccc taataaaaat
    44401 tttgatacat accgcataga catctcctcc atttaaaaag tataccatcg aaaaccgctg
    44461 aaaaatcata aaaaagtatg aagtttaagg accaacgtag ttatcgtacg aaaattagaa
    44521 gctttttttg ttgggaatcc ggtttcttta acaaagctaa gatagcgagt tgcttggaaa
    44581 agttgctatc ttttttctgt caaaacgact cggaatgttc gttttttgtt ctaatttctc
    44641 caagttgttt actttttgat actaagttta tgaaaaatca agaaatagtt gttatactag
    44701 gtaatgctga caaacgggat tggcatttca tcttggttca gctttttttc caattactag
    44761 gaaggaggga tagattgttc aagacttatt ttcgtttcaa taaaatacaa gataatcctt
    44821 ttcgtatcat gaatctgatg ctacttatct ttattgtatt atggttaggt tacataaatc
    44881 atgatttttt gatttcaagt ttttcatcat tagggatctt tacttttttc tattatcaaa
    44941 acataccgat gaaatatttg ctgaaacgta tgtttctgat cggttgcgga ctgttggttg
    45001 ctttcgtttt ggggatctta tcgacttatg ttttatggct tgaaccattt gccgtagctt
    45061 tggttgcttt ttccagcaga ttcgtgttgc gattattcca tatttctaaa ccaggtggct
    45121 tgttttttgc gatgctttct gccatgggaa cgagcatgca gctgccaatc gctcaattac
    45181 cgatcgtctc actttatttt tttatgggtg tcgtttttgc cctgattgcg gcagtgatta
    45241 caaaattact ggattcacgg cctgagcaga caattgaaaa agcaacttta aaagaaagat
    45301 ttcacgaaga gcctttagtg atcattgatt ctgttttcta cagcgctgcc ttatttcttt
    45361 ccgtttatgt cagtcacggc ttgaatctgc ataatcctta ttggttgacc ttgtcatgtg
    45421 catcgatttt gctagcagaa aacttggatg cgatgaagca tcgacaagtc caatatctga
    45481 ttggatcgat gggtgggtta tgtgtgtcgg cattcttgtc atttgtgccg tttacgcagt
    45541 tgcagaccat ttttctgatc acttttctat atggaatcgc acaatttcta gttgctagaa
    45601 attatgcagt tgctaatatt tttcttaatc cgatggcgtt gatgctatct acacttattc
    45661 gtggagcata cttgatttct ttgatcgaat atcgtttttt agggatcgtc ataggaagtt
    45721 tcatcggttt aggtgttgcg tgggtaatga cggttggttt gcaacattat ttaacggttg
    45781 ttcgtcaaaa attagaatag aaggacaaat atatgtgcca aaagaggtta tgacaagatt
    45841 tttgtcacaa cctctaattc aaggtgttca aaagccagat tctcagcatt aagtcaaatt
    45901 cacacaaaaa tgtggaaagg aactttcaaa aaaattttat atttattcta tcaaaaaatt
    45961 ttgtttatga tggtaactga ataaactttc ctgagatggt caatgctttc cttgaaagtc
    46021 ttacagatat tctttcttca tagaaagttt aagttttata gttagtgtac aataaaagta
    46081 tgtttatacc atatggtgtc cggataatca gtcatttaaa caatgaggtt caaagatatg
    46141 aaaaggggaa atttgatgaa gaaaataagc aatgaaatcc aaattctggc agaaacttta
    46201 actcttccca aagtcgttat agagcaaatg attgtacaag caaaaaacat ccttgatgag
    46261 gtgtttgagc aatatagtca tgaactaatg gatcctaata aagcaaaggc gactgcaaag
    46321 aaaatagaca aagtctatga agaggaacct tttgcagcat tagctattta tctatatgca
    46381 gcttctcaaa gttggaaaaa aatttatcaa ccattgaaga ttccaagaac tatctatttg
    46441 gcaacaatga atgcttttac tagatttatt catgaacatt tccaagtaac aggaagctat
    46501 cgctttgacc gtggtttttg gatttggcat tacactagtg gcttgatctt tcggattggt
    46561 gaattggagt acgaaatgac atattttccg aaagggtaca cggatctaag attagaagga
    46621 aagcagtgcc tttctcttca tattccttcc gatgcagatt tatctcagaa taagataaga
    46681 gatagttacg aaagtgccat tcgttttttt gaactttatt ttcctgatta ccattaccaa
    46741 gtgatgtaca cagatacttg gttactatca cccaatctta ctaaatggtt gaaaaaagag
    46801 tcaaaaatct gtctttttgc agccgattat cgattattat cagttgatga acaggacgat
    46861 agtggagtcc cttggatctt tggacgagta gatgcccaaa tacatgatta tccagaaagt
    46921 acgagtttac agcgacaagc aaaagagcaa ttgcttgcag gcgagcacat tggctctggc
    46981 ttaggtattc ttacttcata agtttagtac ttcgaaatag tttaaaagag cgtgagacaa
    47041 aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacggcggaa gcagaagcaa
    47101 ctccttcgga aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaatttctt
    47161 cttatttctt ggagttaaac gcttctgtcc cagcctcttt tttagtgttc tatcgaacac
    47221 acagtacgtt caataggtaa actagctata acaatagata ttaaagttaa cggacaagag
    47281 ggcctttttt aggtagctaa aacgtaataa tgaaaattta tttccagaga taggagtcaa
    47341 tcagctagtc tgatcgactg ttttaacaag ttgactttca ttttaacttt tgataccatt
    47401 taaaacacaa atcgtaattg tttcgatttg tgttttttag catgactaca atcaaagaag
    47461 aaagtgggtc gtccatatgg aaaaaacaga tatctcaagt gcataccgcc gcttgaaaag
    47521 cccaaatatc aaaacacgaa aacgtgcgct gaagatcatc aaagatgtga aacggaatag
    47581 tgggaaaagg taagaagaag gaaacttaat taaaaaaata gccgaaaaac gtttttcggc
    47641 tattttcaat aatcggtttt tgtgtaaggt ttccgaaaga actgctgttg attttccatt
    47701 tttaacatat gataaattgg tagaatcttt cttttcaaat tgataccatt ccctaattcc
    47761 agttatatag ttaactacag aaccacaaag cacgtacgtt tataaaagta ttatctgtag
    47821 ttatttttca attccctttc aacctcttat tttgaccagt agtattgatc actttacctt
    47881 ttaacacctt tgttcgtttt tgaaacattt ttagtcaaga atggaaacgt aactacataa
    47941 tcaaaggttt tgcgaaatat ttcatgaaat atttcatgta gaagcgagca atacggttgc
    48001 aaataaccat tatcgtttaa taagcagtta cgaaagctcc caatatctct aacgttcagc
    48061 tcactccatt tcttttcttc aaaaaaatca gtgattcgta tctctttcta tttgtcgaaa
    48121 aagttaactt taaaaagtaa cgccgaaaaa atatgcaaaa aaataaaaaa gcagttacta
    48181 ttgctagaat aacatcaagg aataataagt tttggttttc agataatagt cgataagttg
    48241 tgtattggaa ttttagatac agtaataaag tatttttcat gaaattttgt gagaatcact
    48301 attacaagta tctttaataa aattaatggt taaggaggaa cttaaagtag aatgtaaatg
    48361 aatagttgta gtcagtcgat tgtgatacta gactcaaagt tgcaggaata agtttttcaa
    48421 tttgtgattg gaagataaaa aatgaaaaag aagggctttt ttttatactt tgttgattgt
    48481 agtgtttatt ttccctctgt ttagccaagt aacattttct atgactcaac cagtttcaag
    48541 tgaggaacat gataaccttt ttcgtgctac agaagagttt aagaaatatc aggaaaatat
    48601 tgttccggag actccattag ctagtgagcc aattactgat attgatggaa accaaatagg
    48661 atggtatata cagtacgaag ttatggtggt aaataatgac tcaatagtaa aaaatacagt
    48721 aggcttagag ctaacaccga ttgaaaaggt gagttcattg ttaacgtata tttatactga
    48781 tgaaagtgac gagactgaga tcattttaac ggattactca aaaattgcta ctgataaaaa
    48841 gtatatattg tggatgtaga cactagtgaa atactttatg atatagaaat tgatgaaaat
    48901 ttactagatg tagcacaaga aatcgatcat caaaagcagg aagctattcg atatgctggt
    48961 agaaaaggga aaagaaaata tttattacgt gaatgtagca aaagttagag aagatgaaaa
    49021 tgagtggaaa gaattcaaga gtcgctactc aattaactcg actccgacat ttacagttta
    49081 cagagaagga tcgatagaaa aaactgtttt ttggactaaa gaaagtggaa tgtctttagc
    49141 tgaagtagaa gagtttttgg attatgttag tatgcaacag taagggagtt cttattatga
    49201 aaaattttga cacatattat gttattgttt tttcgatatc tttgctgtta atattactaa
    49261 ctttaatgca gtataaagag tatccagagt attttatttt aacttttgta tatttagtaa
    49321 gtagctacac acttctatat aaattcagaa gttcttctag agtttctaaa gtttgtttct
    49381 taattagctt attgattcta taatattttt gatttatata ggaaattaaa aatattatag
    49441 aatcaacact cagagaacgg gttggttttt aatagattta ataagtactg ataatgaaat
    49501 ttctcctggt tgggaaaacg aagttggtta ccctgtagaa gaattttttc aagattactt
    49561 tgcggagtat gccgatagga taatgagaga ttacgtaaca gataaaactc aagaagtcat
    49621 caatcaatcc cagtcattaa atgaattatc tgataaggcg aaagaagaag cagttgccat
    49681 tatttcttca gggattagtg actatattga tatgagtggt gaaatcaatt atggcgataa
    49741 agacaaaatt gtacgcatga taaaagcttt tatcaaaact tttgatgtga aggcagatct
    49801 taagtttaat gatacttacc tagtaggaaa attaattaat atccttggga cagagaaaga
    49861 tacggaaacg tttatttcct ctttgtctat gttgtttaca gataagcatt tttcaactca
    49921 gcttggtgga gaggaactag tggaaatctt ttaaccatta cgcccagcat taataaaagt
    49981 atatcaaaaa agtactcctg atgattttta agattggttt gagaaataat gtgaaaacac
    50041 tatttttaat gttaattata gatagagatg aacgcgtcat atggttaaaa gaatgaaaaa
    50101 gtcaaagata cactgctata tttcactaag gatatagcag tgtatctttc atgtcgtttc
    50161 actggcagtt ttttataaaa ggaggtgtgc atttcagttg cttttctttg gtagttagtt
    50221 gaatcgttta agaattaacg gatgtggcag acaggtgctc tgtccaaaaa ccagtaaaat
    50281 ctttgatggt tccatcttta tttagctgtg cctgttcaaa tactgttttg tttttaccga
    50341 atggatcaat tgcaataatt gttccgatcc ccgcacgttt ggcagcaagt aatcctgagt
    50401 aagcatcttc gataacaaga caatcagccg gagctaagct cagtttgtct gccgctttaa
    50461 gaaaaatatc cggttgcggt ttcccaggga aagagccgtc gtcatagaca atcttgtccc
    50521 agtcaaacca gcgggctaaa tcgaaaatat caaaataaaa agcaacattc tctttgacag
    50581 tagccgtggc aatcgttaat ggaatctgtt ggtcagtcag ctggtcaaga gtcgcttcaa
    50641 gcccttttgt caactgaagc tcgttttcat tttgcaagca aagcatacga taatagcttt
    50701 ctttttcttc tgacaatacg gcgatctcag aggcggtcaa atgctctgaa atgaaatgtt
    50761 tcaaaatctc cgtattcgtc cgaccatgaa tattttttaa aatatcctcg tctgataaac
    50821 catcttttgt atgatcatgt atcatatgca gccaagcagc ttcatgcaga tgagagtcca
    50881 aaaacattgt tccgttgaaa tcaaatataa tccctttcat agttacgctc ctatagtggt
    50941 attttcggtt gaatcagcaa tttgcagatc gacatattgt gcatagtgct cttgaacctc
    51001 ccgcgagatc tggcgatccg taatgagcag atcgaaatca tgcaaattat aaaacacata
    51061 aaaatcatct cgattgaatt ttttattatc aacgagtaga tatttcttac gggaattatt
    51121 tagagcgatc tgctgtgctt ctccttcttc ggtactgtaa gtagagatcg cttcgttgtg
    51181 aacgccattt gaactgataa atgcttttgt aaactttagt cttcttaaat tgtcgttggt
    51241 gatcggtccg acaaatgcgc cggtattttt ccggaaatca ccgccaatca gtagaatttc
    51301 tggtccatct gcttgagcaa gaatatcaaa aactggataa ttatttgtaa tgatccgaat
    51361 tttcttgttc agtaaatgcg tcgccaatag ttcgattgtt gtacctggtc ctaagaaaat
    51421 cgtttctccc tcgtgaatca aggaggctgc tgtggcagca atttcttttt tttcttgaat
    51481 ctgcagagtg gctttttcag tgtgagaaag ttcttgatct aaagtgtagg aaagactttg
    51541 agcccctcca tggacacgca gcagcttgcc cgatttttct aattcatcta aatctctgcg
    51601 aacggtcata tcagaaacat ctaattgatt gatgatttca ttaacggtaa ggattccttt
    51661 tttattgacc atttctacga tagtaagcaa tctttctttt ttcaacattg tcttcacctc
    51721 taatttaagg ataatctatc atataaacgg acaacagtca atctaattcg aacaaaagta
    51781 aacatttagg gtggttttta tgtttgttac aatagatttt attgctgttt tctggttttt
    51841 atgattattt gtgtttgttt ttgttgacaa atgtttcgga tgcgtttaca atgcaatcag
    51901 ttagaaagag cgaaaactga ttatagattt gaggagagat gtacatgctt aaattaccag
    51961 aagattttat ttttggcgga gccacagcag cctatcaggt tgaaggagcc actaaggaag
    52021 gtggcaaagg cgctgttgct tgggatgact ttttagagga acaagggcga ttcagtcccg
    52081 atccggcaag tgatttttat catcaatatg caaaggacat tgaattgtgt gaacgttttg
    52141 gtgtaaatgg tctaagatta tccattgctt ggagccgcat ttttcctgat ggagctggga
    52201 aaccaaatcc agaaggaatc gctttttatc atcgtgtgtt cgaagaatgt aagaaaagaa
    52261 acgtaacgcc gtttgtgacg ttgcatcatt ttgatacccc taaacgtttg tttgaccatg
    52321 gagatttttt gaatcgagaa acaatagaag catttgtttc ctatgccatt ttttgttttc
    52381 atgaattcaa agaagtcaag gtgtggagta cctttaatga aatctatcca gttgcaacca
    52441 atcaatattt gttaggtgtt ttcccaccag gaatcaaata tgattttaca aagatcgtcg
    52501 cctgtcttca taatatgatg gttgctcatg cccgtgtagt gaactatttc aaagaaaacg
    52561 aacttccggg tgaaattggt gtagttcact cattagagac gaaatatgcg gcaacagacg
    52621 cgccggaaga taagcatgct gcctttttag acgatgcatt gtcgatccgt ttcttattag
    52681 atgctaccta tttaggctat tattcaactg aaaccttgac tgctcttgat gagatatgtg
    52741 aagcaaatca ggccagctat cattttcctg aagaagattt cgttgaattg aaaaaagcat
    52801 ccacgcgtaa tgactactta gggatcaatc actaccaatg ccattttgtc aaagcttacg
    52861 atggagaaaa tgcaatccat cataacggta cgggtgaaaa agggacctct gtttataaag
    52921 tgaagggaat cggagaacgt atttataaag aaggaattcc tcgaactgat tgggactggc
    52981 tgatttatcc agaaggtcta tatgacttgt tgctgcgaat taaaagcgat tacccacatt
    53041 acaacaaaat ctatatcact gagaacggga tggggtataa agatcagttt gaagatggca
    53101 ttattatgga tcagccgaga atcgactatt tgagagtcta tttggaatcc ttaagcaagg
    53161 cgataacagc cggggtaaat gtgaaggggt attttttgtg gtcgcttatg gatcttttct
    53221 catggacaaa tgggtataac aaaaggtatg gcttgtttta tgttgatttt gaaacccaga
    53281 agcgttaccc aaaagaatca gcttactggt acaagttggt cagtgaaacc aaaacgatta
    53341 tttaataagt gataatggag gaagaaaaga tgaataaaga ggaattacaa atgcttggtt
    53401 ttgagatcgt tgcatattca ggcgacgctc gcagtacatt attgaaatta ttgaaagaag
    53461 taagacaagg aaactttgaa catgttgatt cggcgttgaa agaagccgac gaaaatttaa
    53521 cccttgcaca taattcgcaa acaaaaattt tagcggaaga agcatctggg aaagagatgg
    53581 agatgggatt catttttatc catgggcagg atcatttaat gaccacttta ttgctgagag
    53641 atctgattca agactttatt gtactgtaca gacagaatca aggataggag aagaacgcta
    53701 tggatggaat cattaaacaa atcgaaaaag ggaaaccatt ttttgaaaaa atctcacgta
    53761 atagttattt aggagccgtt cgtgacggat ttttaacagc gatgccagct attttatttt
    53821 caagcatttt cattttgatt gctgcggttc ctgagatttt tggttaccaa tggcctgaat
    53881 ctgtctccac atggctatgg aaagtctata attattcgat gggtgttgta gggttactcg
    53941 tttcagcaac cactgctcgc tgcttggctg attcgatgaa tcgtcgctta ccgaaaaaca
    54001 aagtaatcaa caccacctct gtgatgctgg cctctgtggt aagtttcctg ttgttaagcg
    54061 tctctccgat tgaaggtgga ttttcaacgg aatatatggg gacaaaggga ctactggctt
    54121 cctttgtcgc tgcattttta acagttaatt tatataagtt ttgtgtgctg aaagatatta
    54181 ccattaaaat gcctaaagaa gttcctggaa cgatttcgca aaccttccga gatatttttc
    54241 ctttctcttt ttcagtattt gcggccgttt taattgattt aggcattcga caaatcttcc
    54301 aaatctcatt tgcagaagca atcattacgg tgctgcagcc gctatttaca gcagctgacg
    54361 ggtacttagg aatcgctatt atctggggag cgatggcgct gttttggttt gtcggtgttc
    54421 atggaccgtc gatcgtggag ccggcaattg cggcaattat ctatgccaat gtggaaacca
    54481 atctgcaatt gttcaattcc ggtgaacaag cttctaaagt actgacggtg ggtttaggga
    54541 attttgtagg aactatggga ggaactgggg caacattggt tgttcctttt atcttcctac
    54601 tctttgctaa gtctaagcag ttgaaggcag ttgggaaagc ttcctttatt ccagtaagtt
    54661 tcgctgtcaa cgaaccattg ctatttgcgg cacccatgat tttgaaccct tattttttta
    54721 ttccattttt gttaacacct gttgtaaacg tttgcctgtt caagtttttt gtcgatgtat
    54781 tgggcatgaa cagctttatg tacgttttgc cttgggcaac tccagcaccg atcggtttgg
    54841 ttctaggaac gggcatgggt atcttagctt ttgttctagc agcggttcta atcgtcgttg
    54901 acttcctgat ctatctacca ttttgcaaag catacgatgc tgtcttagta gagaaagaag
    54961 cacaaaaagc agaggtgatc gcaaccgagg aaggtgcttc tgccgatgta cgtgaggaag
    55021 tacctgtggc agcgagtcaa gttatcagcg atgcaaataa agaaatcaat gtgttggtac
    55081 tttgtgctgg cgcagggaca agtgcgatgt tagctaatgc tttaaaaaaa ggtgcatctg
    55141 aatcgaaagc accgatcagt gcaatggcgg gtgcttatgg ttctcattac gaaatgctgc
    55201 aggattttga tatggtggtc ttagcaccgc aagtcaattc ttactatgat gatatcaaag
    55261 aagacaccga tcgcttagga atcaagctgg ttgcgacaaa aggagcagaa tatatcggat
    55321 taacgagaga tcctgaaaaa gctgttcagt ttattctgaa ccaatttcaa taaaggagga
    55381 gccataatga aaatgggaat ttggttgcgt aaagctgttt tatttgcagc tgtgttaggg
    55441 gctttggggg ctgtttcaaa aactgttgaa gcatctgcca gcgaatttat tcgcggagca
    55501 gatatttcga tcctgtcaga tatggaaaaa tctggtgcaa aatattatga agacgatatt
    55561 tcaaaagatg ccttagagat tttaaaaaat aatggtgtca actatgttcg actgcgtttg
    55621 tggcaggatc cttataatag taatggagaa agttatggag cagggacaaa tgatctggaa
    55681 acgacgattg accttgcaaa acgagcaaaa aatctgggac ttaaggtgct gctggatttt
    55741 cactacagtg atttttgggt tgatcctggg aaacagaatc ttcctaaagc atggcaaggt
    55801 ctgacctttg aagaaatgaa tacggcattg tacgactata caaagaacgt tctgtcggaa
    55861 atgaagcagc tggacgttta tcctgatatg gttcagatcg gcaatgaact gaatagcgga
    55921 atgctctggc cttatggcaa gtcttgggga gaaggcgggg gagaatttga tcgattggct
    55981 gcttttttga agtctggtat ccaagctgtt cgagacactc aacctaaaac aactccagta
    56041 atgctccact tagctgatgg tggcgataca ggagcattta cttggtggtt tgatgaaatc
    56101 acgagtcgag gtgtgtcatt tgatttaatt ggagtgtcgt attatccgta ttggcacggt
    56161 tcaatgaatg aacttcaaac caatatgaat gcgatcagcc aacggtatgg taaagaagtg
    56221 atcgtagttg aaacagctta tgggcataca acagcgaatg ccgatactat gccaaatgct
    56281 tttggtgaag ccgaagcagc tgcgggggga tatgaaccaa caccgacagg acaagcagag
    56341 tatttgctgg atcttgccga tcggattcag gcagtaccga ataatcgcgg ggcgggcttc
    56401 ttctattggg aacctctttg gtacaacgga aatgtgagtt gggcaacaca agcaggtatg
    56461 aattatctca gtgttcaatc cactatggga aacgaatggg acaaccaagc gatgtttgat
    56521 tttgctggaa acgccttgcc aagcttgcgt gcgtttaaac aagcaggggc gcaaataaat
    56581 ctagttaaaa atgcaagttt cgaaagcgat ggaccaacga ccagtccttc caattggcaa
    56641 acctggtttc aacaaggaac cgctaagggt acgatcaaaa cggaagcggg cacttttttt
    56701 ggggactaca aactaacttt ttggcaggat caagcatacg aggcatcagc ttatcaaaat
    56761 gttagtggca taagcaaagg aacttacaca ttgagtgcct atgtaatgtc tgggggagag
    56821 tttgatacta atcagttata tatcaaaaat tatggtggag cggaactaaa tactgcggtt
    56881 gttaaaacaa gtgaatggac aaaaattaca atcaaagaca ttccaattac aacaggtgtt
    56941 tgtgaaattg gggtttatag caaggcttca ggaaatgctt ggacgaacgt tgattttgtt
    57001 tcattggtga aacaatagac tgcggatcgt ttcgattcaa gatcggaggt tcccttcagc
    57061 cacatcgtta gcgtagaagg caggaatcga acctgcacct cgcctttgcc aaaaataacc
    57121 agaaacgatt tccagtcatt tttaccaaaa cgagtaaagc accagacttc atctacactt
    57181 tgtattatgc taaagagagt tcaacgattt cgtaagaagc aatgaaaaaa ttgcaaaaag
    57241 ttgtgcagat aacataaagt ttgtttaaga aagggtaacc ttagaattcc gtcgtgaata
    57301 tgatttgagc acttagatgg atagaaaaaa gttctcggaa aacagttcgt ttttcgagaa
    57361 cttcttttta tgataagaat gaaacaaatt aacttatatt atagtctctt ttggtaggga
    57421 accatcttat ttttaatgaa aatcaagcat tttagctatt gaagaatgtt gatttatagg
    57481 gattccccct ccatattatg ttggctcttt attcccactc cactgttgct ggtggtttag
    57541 aagtgatgtc atacacgata cggttgacgt gggctacttc gtttacgata cgtacagaga
    57601 ttttttgcaa gacttcccat gggatacggg caaagtcagc ggtcattccg tcgattgaag
    57661 ttacagcacg gataccgact gtgtagtcgt aagtacgtcc gtcacccata acaccgacag
    57721 agcggatccc tggaagaaca gtgaaatatt gccagatatc acggtcaagt ccagcagctg
    57781 cgatttcttc acgcaagata gcatcagatt cgcgtacgat ttctagcttg tcttcagtga
    57841 tttcgccaag aacacgaata ccaagaccag gaccagggaa tggctgacgc caaacgattg
    57901 aatcaggcat gcctaactga gtccctaatt cacggacttc gtctttgaat aatgtgttta
    57961 atggttcgat caattcaaat tgcatatctt ctggcagtcc acctacatta tggtgagatt
    58021 tgatcgtctg tgctgtttct gtaccagatt cgataacgtc agtatacaac gttccttgtg
    58081 ctaggaaaga gacgccttct tcaccagcaa gttttgttgc ttcgtcatca aaaacatata
    58141 cgaattcatt cccaatgatt ttacgttttt gttctgggtc agatacacct ttcaacttgt
    58201 ctaagaagcg atctcttgca ttgactttga tgatgttcaa gccgaatttt ccgcctagac
    58261 tttccattac ttgttccgct tcgcctttgc gtagcagacc gtggtcaaca aagatagatg
    58321 tcaattggtc accgatcgct ttttgtaaaa gaaccccaac gacactagaa tcaacgccac
    58381 ctgaaagacc aagcaagact tttttgtcac caacttgttc acggattttt tctacttgca
    58441 tatcgataaa gttatccatt gtccagttac cttcacagtg acaaacatca aaggcaaagt
    58501 ggcgcagcaa ttcgattccg tattcggaat gacgtacttc tgcatggaat tggataccat
    58561 aaaaattacg tgttgtgtct tggatagaag caatcggaca atctttgctt gttccgactg
    58621 tttcaaagcc ttcaggaact ttagttacca agtcaccatg gctcatccat actgtttgtt
    58681 gttttggtgt ttctttaaac aagacagctt gatcgcttgt tacttctaac aaggctttcc
    58741 catattcgcg gtttttagca ggctctacgg aaccgccaag tttgtacgtg atcaattgca
    58801 taccatagca aatccctaat acaggaatac ctaaattgaa gatctcagga tcgatcccaa
    58861 atgcattctc atcatataca ctgtttggtc cgccagaaag aatgattccc tttggattca
    58921 tcgcttttac ttcttctgca gtaatgcggt ggctcaataa ttcggaaaag actccaagat
    58981 cacgaatacg tcttgtgatc aattggttat actgactacc gtagtctaat acgataatct
    59041 tttcaacgtt cgtcatatcg gcaacgtttg tcaaatttat tacccctatc cctttaaatt
    59101 ttatctcaaa atagtcaaac tattttaaaa tgctaatatt ttcgcatgaa aatcgaatcg
    59161 atgatatgat tttcagtttt atggagaatt atatcggctc ttcctcgtgt aggcaagata
    59221 tattcctgta aatttttcaa attcacattt ttccatacat cttttgccat tttaaaggct
    59281 tcttcacgat ctccgatagc aaaacgataa taataattct ctggattttg gaaagccgta
    59341 tccagtaacg caccaaaacg atccaaatac catttttcga tcagcacggg atcggcatcg
    59401 acatagacag aaaaatcaaa aaagtcgctg acgtagattt gctgattcgc aggtaattga
    59461 agcgtattga tcccctcaac gatcaagata tcaggctgct ggatcaattc gtacttgtct
    59521 ggaatgatat catacacatc atgtgagtaa acaggtactt ttatctcttc ctgaccagat
    59581 tttacacggt tcaagaaatc gatcagcatc tccatatcat aactttcagg aaatccttta
    59641 cggtccatga ttccacgctc ttttaatata cgatttggat aaagaaatcc gtcagtcgtg
    59701 atcagctgaa cgtttcttcg tttgaaggta cgagacaaaa tcgtctgcag cagtctggct
    59761 gttgtacttt ttccaacggc tacacttccg gcgatcccga taataaaagg cggtacttct
    59821 acgtatttat gaagaaataa acctttactc aagaacaaag attcaaattc tttcatataa
    59881 agataaatca gatgagcaag aggtacatag atatctcgaa catcctgcat ggaaatgcgg
    59941 tcattcaaac ttttgatatt atctagttca tcttgtgtca aaggtgcttg cccattacga
    60001 taaaattctc gccattcttc tcttgccaca cggtagtagt tcattggatc attcatatta
    60061 gcctcctgcg ttctcccatc aatggaacta ttttaacaca aaataaacgc aaaccccacc
    60121 acttaagaga gattgccgat ctttttgtat aatctttata atagaaagaa acggagtctt
    60181 attcttgact tgcgcacgga tacataaaag gatagactgt tgtcatgaaa gaaatgagaa
    60241 ggatggaaga catgacaaat cattactatt ctgaacgccc agatacggcg cacgattttg
    60301 aacaatgggc ttttgagtta aaaggcagaa cctttcaatt tgtaacagat tctggtgtat
    60361 tttcacgtga aacagtggat tatggttcac gtgtcctgat tgatgctttc gattgggaag
    60421 agttacctga tgaaggaaag atcttagatg tgggatgcgg gtatggaccg attgggttag
    60481 ctttggcatt tgcgacacag cggtttgttg aaatggtcga cattaatgaa cgagcggtaa
    60541 gtctagcgca aggaaacgca aagcgtaacc aaatcgaaca tgttgatatc catcaatcga
    60601 atatctatga agcagttcat gaagaaacct atgcagctat cgtaagcaat ccgccgatcc
    60661 gtgctggaaa aaaagttgta catgaaattt tgacaggcgc atatcctcgg ttgaagaagg
    60721 gcggtacact gacaattgtg atccagaaga aacaaggagc gccgagtgcg caaaagaaaa
    60781 tggaagaaac atttggtaat gcagaaatcg tcacaaaaga taaaggatat tatatcatca
    60841 aaagtgtgaa agaataacta gctgaaaaat agtggagcga cgaaagagtg gaactgggcg
    60901 tgcatgtacg ggtacgtatc agttcttttt ttgttgagaa gtttttaaaa acaaagaact
    60961 attttttctt caaaaagaaa tagtaggaac agagtcgttg ttttttacga taaaataaat
    61021 aagatacaga acttgtatag aaggctgcca agtcggtaga taaaggaggt ttttatcaaa
    61081 aaacatactt tccttttttc tctattcttt tcttgggggt tttggtacac tgatagggaa
    61141 tttagataga agggagccaa taggatgaag ctgaccatca gagatatcgc agaaatggca
    61201 ggcgtatcag taacaactgt ctcacagatt ttgaataaca aaggaagcag atttagtgat
    61261 aagacaagaa aaaaagtgtt gtcgatcgtg aatgaacatc attataagcc cgattatttc
    61321 gcttcaaatg tgatcaatcg acattcaaag accatcggaa tgatcgtccc tgatgtcact
    61381 gatttcttct tctccaaact gatcgaaggt gtagagagct atttgaatcc tttgggctat
    61441 gtgatcatgt tatgcaattc tcgtcatagc caagaaaatg agatcaagta tttacaagag
    61501 ctttcccacc gctcagtgga cggaatcttg ctggccacac caaatatttt acctgagaaa
    61561 tatgcattag acagcggttt ttatcaaaaa atgccaatca ttttaatcga tcgcggattg
    61621 aatcgtcggg ataacggacg gcttatcgta aaggaatatg agggcgccta tcaagctgtt
    61681 tcgctgttta ttgaaaatgg ccatacgaag atcggcatgc tgaaggaaac cactggatac
    61741 tatcaattag aagaacgttt caacggatat cgtcatgcgc tgaaagatca tggcctgcct
    61801 tttaatggga aatttgttga acatggagag ttgactgtcc aaggtggata tactgcatca
    61861 aaaaaattac tcaaacataa agatatcacg gctatttttt gcgggaatga tgccatggcg
    61921 atcggatgct atcaggcgat cgatgaattg ggcaaaaaaa tcccagaaga tatctcagtc
    61981 ataggattcg atggattaaa gctgtcagaa tacatgattc cgcaattgac gacagtgcag
    62041 cagccaagtt ttgatattgg attttatgct gcacgtttcc tgatcgatac gatcgaattt
    62101 cctcaaagaa aagttccgaa caaagtgttc gaaacaaaat taattattcg tgagagcgta
    62161 aaagtattga caaagggcta actcggcggt atattgctta tgtaagcatt tacaaaggct
    62221 agttagtgct ttttttattt ttagtaaacc agtttactaa agtggtttac ctttcctaaa
    62281 aataatgtat aataccgaat gaagacaagg aggatcaggc aatgcgaatg gtggatctga
    62341 ttgaaaaaaa gagggatgga aacgagctgt cgaaagagga aatcgagtac atcgtcacga
    62401 actatacgaa tggaaagatt ccagattatc aagtcagtgc attattgatg gcaatttttt
    62461 atcaagacat gacaaatgaa gaaatcacga acttaacatt ggcgattgct aattctggtg
    62521 atgtcatcga tttgagttct cttgaaggaa taaaggtaga taagcattcg acaggtggcg
    62581 ttggtgacac gaccacattg attttagccc ccctagtagc tagtgtcgga gtgacagtgg
    62641 ctaaaatgtc tggacgcggt ctaggttaca caggtggtac actagacaag ctagaagcaa
    62701 ttcccggatt tcagatcgag ctttcggatg aagaatttgt acgcattgtt aatgaaagta
    62761 aagtagcggt aattggtcaa tcaggaaact tggcaccggc ggacaaaaaa ttgtatgcct
    62821 tgcgtgacgt cacagcgacc gttgattctc ttccgctgat tgccagttcg attatgagta
    62881 aaaaaatcgc ggcaggagca gatgcaatcg ttttggatgt gaccactgga gacggtgctt
    62941 tcatgaagaa catcgaagat gcaagacgtc tggcaaagac catgacgagc attgggaaac
    63001 tggctaaccg agaaacagtg gcggttatct cagatatgtc agaacctctt ggcgaagcta
    63061 tcggaaatag tctagaagtt gtcgaggcaa tcgaaacatt acaaggaaat ggtccagaag
    63121 atctagtaga aatgtgctac gccttaggaa gccaaatggt tgttcttgct ggcaaagcta
    63181 agacaaccga tgaagcaaga acgttgcttc aagaagctct ggaatcaggt aaagcgttag
    63241 caaaattcaa agaaatgatc caaaatcaag ggggagaccc agcgatcgtg gaacatccag
    63301 aacgaatttt gactgcacgc tacacaatgg agcttcctgc taaacaaagc ggagttgtgt
    63361 caaaaatcgt ggcaaatgaa ttaggaattg ctgcaatgat gctaggtgct gggcgtaaaa
    63421 ccaaagaaga agatattgat cacgctgttg gattgaaact gcataaaaaa attggtgaca
    63481 ctgtgacaaa aggtgagtca ctattgacga tctatagtaa cgacgaagaa atcacttctg
    63541 tcattgaatt attatataaa aatattgaaa ttggtgaatc ggcaatgaag ccaacgttga
    63601 ttcatgacat catcacagaa taaaggaaaa aagcggactt taccgtttta aagaaaaggg
    63661 gaaaagaaat ggaactaaac cgaatgatcg accatactat tttgaaagca gacgcatcta
    63721 aagaagctgt gatgcaaatt atcgaagaag caaaaaaata ccatttttat tctgtctgca
    63781 tcaatccagc atgggttgcg ctagcgaaag aacaattaca aggtgagcca gtagccgtat
    63841 gtacagtaat cgggtttcct ctaggagcaa acacacccga gacaaaagca tttgaaacaa
    63901 cagatgccat caataacggt gcagatgaag tcgatatggt catcaatatc ggtgctttga
    63961 aatcaaagga tgatcaacaa gtccaaaaag atatcgaagc agttgttgaa gcagcaaaag
    64021 atcgtgcgct tgtcaaagtt attgttgaaa catcgttatt agatagagaa gaaatcatcc
    64081 gagcttgcga aatagcaaaa gctgcaggcg ctgattttgt caaaacatca actggtttct
    64141 caacaggcgg agcaaaggta gaggatgtcc gtttgatgcg tgaaactgta ggaccagaaa
    64201 tgggtgtcaa agcttccggc ggtatccata acgaagaaga agcaatggca atgatcgaag
    64261 ctggtgctac aagaatcgga acaagcgcag gagtagcaat cgtatctggt tcaactggtg
    64321 aaggctatta attttttcaa aggagaagct catggcagaa ctcaaacaag aatggatcaa
    64381 cacagctatt gaagcgttag ataaagccta tgttccttat tcccattttc cggtaggcgc
    64441 ttgtctcgta acggaatcag gcaagattta tcaaggcatc aacattgaga atgcatcatt
    64501 tggattaacg aattgtgccg agcgcacagc ttttttcaaa gcagtatctg aaggagaaaa
    64561 aacatttacg catctagtcg tagcggggca cacccccgat cctatctctc cttgtggtgc
    64621 atgtcgacaa gtaatggcag aattctgttc ccctgatatg ccggttacgc tagtaggaga
    64681 caatggtgtg acgaaggcaa cgaccgttcg tgcactgctg ccttatgcat ttactgaaaa
    64741 agatctataa ctcatgttca ttcagtcagc tgactgttga aaaataaaac aattatttta
    64801 ggaggcttta cagaaatgaa aaaagcaaaa ttatttggtt taggtgctgt cgcactagca
    64861 gcagggttat ttttaggtgc atgcggcaac aacggctcta ctgattcatc cggtggcaaa
    64921 agctcatcag acacaacaac tgcagcattg atcacagaca caggtggtgt agacgaccgt
    64981 tcattcaacc agtcagcttg ggaaggacta gaaaaatggg ggaaagatca aggactttca
    65041 cgaggaaatg atggcttcca atatttccaa tcttcaaatg aatcagatta catcccaaat
    65101 attgaccaag cattgaatgc tggattcaaa acgatttttg gtattggtta taaactaaaa
    65161 ccagcgatcc aagaacaagc aacaaacaat acaggtacga actttgttat tatcgatgat
    65221 gtcatcgatg gattagataa cgttgtatct gcaactttca aagacaacga agcttcttat
    65281 cttgcaggtg ttgcagcagc ttatacgact gaaacaaacg tagtcggctt tatcggcggt
    65341 gtaaaaggtg aagtaatcga tcgttttgat gctggattca aagccggtgt tgatgcaggt
    65401 gcgaaagaat taggaaaaga aatcaaagta ttgaatcaat atgctggtga cttctctgct
    65461 ccagataaag gacgttcaat tgctcaaggt atgtatgcac aaaacgcaga tatcatcttc
    65521 catgcttcag gcggtacagg taacggtgta ttccaagaag caaaatcatt gaacgaatca
    65581 ggcgacaaaa aagtttgggt tatcggtgtt gaccgcgacc aatcagacga aggcgaatac
    65641 acattaaacg gcgaaaagaa aaacttcaca ttgacatcta cactgaaagc agttggaaca
    65701 gtagtagaag atttggcaca aaaatcagcg gacggtaaat tccctggcgg cgaacacact
    65761 gtttatggat tgaaagaaga cggtgttggt ctaacagaag gacaactttc tgacgaagcg
    65821 aaaaaagcag ttgatgaagc aaaagaaaaa atcatttcag gtgacgtaaa agttccagaa
    65881 actcctgaag aaaactaaaa ccatttctat agccttgtta gacacatgtt tgacaaggct
    65941 atggtttgat tagaacaaga attgttcatt tatccaagct gccttgtgac agtttggata
    66001 agggaacaac tccctgaagt aatgtttgtt tcaaaagaag aacgctcact aagcgtgaat
    66061 cttagtcaat cgttgcccta gatagaaatc gttaggatgt gaaaaaaagg tggttcaaga
    66121 aaatattgtc atagaaatgc agaatattac caaagaattt ggaacattca aggcaaatga
    66181 caacatcaat ttacaagtaa aggcaggaga aattcatgcg ttgcttggag aaaacggtgc
    66241 tggcaaatct acattgatga acgtgctttc cggattatta gagccgacat cagggaaaat
    66301 tttgatgcgt gggaaagaag taaagatcac aagtccgacc aaagccaacc aattagggat
    66361 tgggatggtc catcagcact ttatgcttgt tgatgccttt actgtaacag aaaacatcgt
    66421 gttgggaagc gagcctagtc gcgcagggat gcttgaccat aaaaaagcac gaaaagagat
    66481 ccaaaaagtt tctgaacagt atggattgtc agtcaaccca gatgcttacg ttcgtgatat
    66541 ttcagttgga atggaacaac gggtagaaat tttaaaaaca ctttaccgag gagcagatgt
    66601 actgattttt gatgagccga cagctgtatt gacccctcaa gaaattgatg aattaatcgt
    66661 gatcatgaag gaattagtca aagaaggcaa gtcgatcatt ttgattacgc ataagttaga
    66721 tgaaatcaaa gcagtagctg accgctgtac agttatccgc cgtggaaaag gaatcggtac
    66781 agtcaacgtt aaagacgtaa cctcacaaca attagccgat atgatggttg gaagaacggt
    66841 ttcattcaaa acgatgaaaa aagaagcgaa gcctcaagaa gtcgttttgt ctattgaaaa
    66901 tctagtggtg aaagaaaatc gtggattaga agccgtgaaa aacttgaact tagaggttcg
    66961 tgctggcgaa gtacttggta tcgctggaat cgatggaaac gggcagtcgg agttgatcca
    67021 agctttgact ggtttgcgaa aggcagaaag cggacatatc aagctaaaag gggaagacat
    67081 cactaataaa aaaccccgaa aaatcactga acatggtgta ggacacgtgc cagaagaccg
    67141 tcataaatac gggttagtcc tagatatgac attatctgaa aatatcgcac tacaaaccta
    67201 tcatcaaaaa ccttacagta aaaacggcat gctgaattat tcagtaatga atgaacatgc
    67261 cagagaattg atcgaagaat atgatgttcg aacaacgaat gaacttgttc ccgcaaaagc
    67321 tttatcaggc ggaaatcagc aaaaagcaat tatcgctcgt gaagtcgacc gagatcctga
    67381 tctgttgatt gttgcaaatc caactcgtgg actggatgta ggtgcgatcg aatttattca
    67441 taaacgtctg atcgaacaaa gggacaaata caaagcagtg ttactgatca gtttcgaatt
    67501 agaagaaatt ttaaatgttt cggatcgtat tgctgttatc catgaaggag aaattgtcgg
    67561 tatcgttgat ccgaaagaaa catctgaaaa tgaattaggt ctattgatgg cgggctatac
    67621 cttagaagaa gcacgaaaag agttagaaaa gggggctggc gcacatgaat gatcgcagcg
    67681 aaaaattccg taatttatta gtgcctgtac tttcagttgt tttaggattg atcttaggcg
    67741 cgatattgat ggctgctttt ggttttcagc ccatccaagg ttattcttct atgctagatg
    67801 cagcccttgg ttcgcaaaga gggataggtg aaacgttaag gcaagcaacg ccgttgattt
    67861 ttacagcatt aggtttttct gtagctaact cagctgggtt tttcaatatt gggctttctg
    67921 gacaagcact ttgcgggtgg atcgtcagtg tctgggtggc acttgcgttt ccagatctgc
    67981 caaaagttgt tttgcttccg ttatgcgtga tcttaggtgc cttagctggt gcgatggcag
    68041 cggctgttcc aggtttactt cgagcatttt ttggaacaag tgaagtgatc gtaacaatca
    68101 tgatgaacta tatcttatta tacgtaagca catttgcttt acatgaagtg atgcctgaaa
    68161 gctatcgctc aagtttggac tcttccaata tgatcagtca aaatgcctca ctgcggttac
    68221 catggttaac acaagctttt ggcggctctc gcgtaaactc aggattattt ttagcaatca
    68281 tcgcgcttat tttcgtttgg ctgctgatga agaaaacgac tttaggattt gaaatccgtt
    68341 ctgttggatt gaatcctttt gcttctgagt atgcaggaat gagtagtaaa cgtacgatcg
    68401 ttttgtcgat ggtcatttct ggcgctttgg cagggcttgg cggtgtggtt gaaggattag
    68461 gaacgtatca aaacttcttt gttcaaacga cttcgctatc tattggattt gacggaatgg
    68521 ctgtttcctt gcttggttct ggatcagcaa tcggtattct tttatcagcg atcttgttca
    68581 gtattttgaa gatcggtgga cttggcatgc agacaggagc aggtgttccg tttgagatcg
    68641 tcaatgtatc gattgctttg attatcttct ttgtcgggat caacttcctg attcgtttcg
    68701 tcatggctaa agtcttccaa gggaaaaaac aagaagagat cgttgcgaca atagaatcga
    68761 aaccaacgaa tcaaaaccat gaagggggcg aactataatg tcgactatcg aattaattgc
    68821 cttgatcctt acttctacac tggtttattc aacaccgcta attctaacgt cactaggcgg
    68881 aactttttct gaacgaagcg ggatcgtaaa cgtagggcta gagggaatca tggtcatggg
    68941 tgcttttagt tccatcgttt tcaacttgac gatgagtcca tcacttggtt ctatgacacc
    69001 atggatcgct attttagtgg gtggcgtcgt aggtatccta ttttctctga ttcacgcagt
    69061 agcaacagtc agtttgcgtg ctgaccatat tatcagcggg acagtaatta atctgatggc
    69121 acctgcttta ggtgttttct tgatcaaagc atggtatggt aaaggtcaaa ctgataacat
    69181 cacgcagaat ctggggtatt tttctttccc aggattagca gatattccgg tcattggacc
    69241 aattttcttt aaaaatacat tcttacctgc ttattgtgct attttgattg ctgtcctagc
    69301 ttgggtgatc atcttcaaga cgaaattcgg cttgcgcctc cgttcagttg gtgaaaatcc
    69361 tcaagctgcc gacacattag gtatcaatgt atatgctatg cgttattcag gcgtactgat
    69421 ttctggcttg ttaggcggta tgggcggtgc tgtttttgct cagtcgattt ccggtaactt
    69481 ttcagctggt acgatcgttg gacaagggtt catctccatg gctgctatga tctttggtaa
    69541 gtggaatcct ttaggggcaa tgggtgcctc attgttcttt ggttttgctc aaagcttgag
    69601 tatcattggc gcacaattac cagttatttc ttcgattccg ccagtattgc tgcaaattgc
    69661 tccatatttg ctgacaatca ttgtgctggt tgttttctta gggaaagctt ctggtccaaa
    69721 agcaaacggg aagaactata tcaaatcaaa ataatctttt agcgaataaa aagggtgggt
    69781 tacgggaagt gtttttcgtc tcgccctttt tgttttaaag gcaataaaaa taaaaagaaa
    69841 gaaggaataa tcatgtttaa acgcgtacat ctgatcgtca tggattcagt aggaattgga
    69901 gaagcaccag atgccgataa gttcggtgat gttggttctg atacattagg acacattgcg
    69961 aaagaagcag gattgaacgt tccacattta gagcagcttg gtttaggaac gatccgtcct
    70021 cttgaaggtg tggaatcagt gaaagatcat caaggctatg cgacgaaatt agaagaagtt
    70081 tctgtaggga aagatacgat gacaggccac tgggaaatca tgggattgaa tatcaaaact
    70141 ccatttcgtg tgtttcctaa tggattccct gatgatctga tcaaacaaat cgaagaattt
    70201 tctggccgta aagtcgtatg taacaaacct tatagcggaa cagctgttat tgacgattac
    70261 ggtgaacacc aaatgaaaac aggcgattta attgtatata cttctgccga tcctgtcctt
    70321 cagatcgcgg cacatgaaga aatcattcct ttagaagaat tatatcggat ttgccaattc
    70381 gttcgtgata taacaaaaga tgatccgtat atgattggcc gaatcattgc acgtccgtac
    70441 gtgggtgaac caggagactt cacacgaaca agcaatcgcc atgattatgc gttgaatcca
    70501 ttcggacaca cagtccttga ttctttgaaa gaagcaggaa aggacgtgat tgctgttgga
    70561 aagatcaacg atattttcaa cggacaagga atcactgaat ctgtccgcac gaaaagcaat
    70621 atggatggcg ttgaccagct gctgaaagtt atgaaaaacg atttttcagg aatcagcttt
    70681 accaatttag tagattttga tgcattatac ggtcatcgtc gggatgtagt cggctatgcg
    70741 catgcgattg aagaatttga cttacgaatt ccagaattgc ttgaagcgat ggaagaagac
    70801 gacttgctaa tgattacagc cgatcacgga aacgatccga cgtttactgg taccgatcat
    70861 acgcgagaat atgtaccgtt attggtttac agtaaaaaaa tgaaagaaca aggggcattg
    70921 cctcaaggat attattcaga tatttcagca acgattgctg agaactttag tgtttcttcg
    70981 actgaaaatg gcgaaagctt cttgaaatta ttaaaataat ctttttaaat aggagaaagc
    71041 aaatgaccaa gttaagcgag atgttacaag aaacgaccca gtttatcctt gataaaggag
    71101 taaaagaaat tgattttggt ttgattctag gttcaggatt aggtgaatta gcagatgaaa
    71161 tcgaagatgc tgtaatgatt tcttatgatg agatcccgtt ctttcctgtt tcgacagtag
    71221 ttgggcatgc aggaaagcta gtttatgggt ctctggcagg caagaaagtg ttagccatgc
    71281 aagggagatt ccactactat gaaggtcatt ccatgcaaac agtgacctat ccagtacgtg
    71341 tgatggccgg actaggggcc cattcagtca ttgtgactaa tgcatgtggt ggtgtcaacg
    71401 aatcatttgt accaggagat ttgatgctga ttaccgatca tatcaatttt actggtgaaa
    71461 atcctttgat cggtccgaat gaagatgcaa tgggccctag atttccagat atgagtcaag
    71521 cttatacgcc tgactatcaa gcaacagcaa aagcagcagc agaaaaacta ggtttggatc
    71581 ttaaagaagg cgtatatatg ggctattctg gaccaactta tgaaacgcct gcggagatcc
    71641 gaatgtctcg gacaatagga gccgatgcag tagggatgtc gactgttcca gaagtcatcg
    71701 tagcagctca cagcggatta aaagtgttag gaatctcttg tattaccaac ttagcagcag
    71761 gtatgcaagc gaatctgagc catgaagaag ttgtagaaac aactcagaga gtcaaacaat
    71821 cttttaaagc attggttaaa gaaacattga cattattgta ataggaggaa caataaaatg
    71881 agtgtgcata ttgaagcgaa agaaggcgag atcgccgata agattttact tccgggagat
    71941 cctttgcgag ctaaatatat tgcggagact tttttggaag atccagtttg ttacaatcag
    72001 gtacgcggta tgttaggctt taccggcaaa tataaaggag aacgtatttc tgttcaagga
    72061 acaggaatgg gaatgccatc agccaccatc tatgcccatg aattgatcca gtcgtatggt
    72121 gtaaagaaat tgattcgagt aggtacatgc ggtgcattgt caaaagacgt ccatgttcgt
    72181 gatttagtac tagcacaagg tgcagcgacc agttcttcga tgatcgaaaa aaatttccaa
    72241 gcctttcatt ttcctccaat cagtgatttc aatctattac tgaaagcata tgagatagct
    72301 aaagaaaagg gctatacggt tcatgtagga aatgttcttt cggaagattc tttctacaaa
    72361 gatgatttga cagagacgtt ccaattagct gaacttggcg ttttaggagt ggaaatggaa
    72421 gcagcggctc tttattactt aggcgcgaaa taccatgtgc agacattaag tttgatgacc
    72481 gttagcgacc atttgatcac cggtgaagaa acaacagctg cagaacgtca atctacgttc
    72541 aatgaaatga tcgaagtagg attggaaaca gcgatagcag aataagaagg aaatgaaaaa
    72601 gctgccctgc attaagtaat aagaacatcc aagcaaaaat agctcctcat gtttttgact
    72661 tggctggact tattattgca aatgtaagct tttgaacacc gtttaccaag aggttgtgaa
    72721 tctgccgttt aactctgagt cacaaggaac aatttcaaaa aacagctttt catgtttgat
    72781 gaatattgtg acttgtgacc gagaagttgg caggagaaaa ccgtttatac gaaaaaaggg
    72841 actgtgacaa atcgtgtcac agtccctttt ttatagtgtt ttttataggt agtatttttt
    72901 agcaactgtt aagtcatcat ttaattcata aacaagtggt tgacctgttg gaatttcaag
    72961 atccatgatg tcttcgtcag agataccttc gatatgttta gctagggcgc gtaatgagtt
    73021 accatgtgca gctactaaaa cagttttgtt gtctagtaat gctggagcaa tttcatcttg
    73081 ccagaatggc aaagcgcgtt ctaatgtcac tttcaagttt tctccaccag gaatatcgcg
    73141 tttgtctaac attgcgtaac gacggtcatt tgctgcagaa ccttcgtcag tttcttccat
    73201 taatggagga agagtatcgt aagaacgacg ccagatgtgc acttgttcgt cgccgtattt
    73261 ttcagctgtt tcttttttgt tcaaaccttg taatttacca tagtgacgtt cgtttaagcg
    73321 ccatgatttg atttgtggta cccaaagttg atctgaatat tctaagatca agttacatgt
    73381 tttgattgca cgagttaaaa cagaagtgta agcaacatca aattcgattc cagcttcttt
    73441 gattttgcgt ccgccttctt ttgcttcttc gattccttct ggtgctagat ttacatcagc
    73501 ccaaccagta aattggttca atgcattcca ttcactaaga ccgtgacgag aaaaaactaa
    73561 ttttggcatg tttagctctc cttttaagat aggtttatat ttctgaaatt ttcagttctg
    73621 ttttattgta caattttttg gaagagattt ccatcgaaaa attagataag attctatgag
    73681 aaaacaagta gagaatataa aaaaacgaac agtgcccacg caactgttcg tttttaggta
    73741 ttagatgcat aattgctcga tttctttttc aggtaaattg atatatttat aacatgttcc
    73801 aactgaaaca ccgcattttg aagaaataaa ttggatcgtt tctttttttt cataatagag
    73861 acgtcggatt tttttgatcg tttttgcatc gatttttggt cttccgccga tttttccttt
    73921 tttacgagct tcatctaatc caactaatgt acgttctttg atcaatgcgc actccatgtt
    73981 tgctaggttt tccattaatt ggaaatagat ttttcccatt tcgcgcgtat cgattccttc
    74041 atcaagacta atcaagtgca gcccgcgatc tttgaataat tgcgtgagtt ctgttaattg
    74101 ccgcgtagat ttgcctaatc gatgaagttg gcaaatgact agtgcatcgc cttgttgcat
    74161 gtcagcgatc actgactcca aatgaacagc agtagaatca ttttgttcca cttcatatgt
    74221 ctcatggaaa atttcttgac aaccaaagtc agataattga tcaacctgag ttgttaaatc
    74281 aacgtcattt atggttgtgc gtgcataacc aataattttc atggtgattc ctttctagca
    74341 gtgattttct aactggatat aacagtaaaa atgtagcata aagtacgtca aaaagatagt
    74401 tttttcgcaa attttccgct gtcttttttt attggatgat caagcagttt ttctttattt
    74461 acgacggatt gcgtcaagaa tctcaacgcc tttttctgag ccgacaacta ctttagaaac
    74521 catgtttagg aaaagcccat gttctactac cccaacaagt tcatctaaat aggtagctaa
    74581 gctttctggc tgttcgatga cttctagatg cagatcaatg atgtaatgac caccatctgt
    74641 ggttaagact tctccgtttt cgtctgtacg caagacgggt tggaatcttt tttcttcaaa
    74701 aatatgaagt agctgttggg agccataagg gatcacttca actgggagtg ggaacttccc
    74761 aagtttatcg actaatttag aagaatcaac gatccaaatc gtttcttttg agtacgtagc
    74821 gactattttc tcgaaaagca aagcagcgcc gccgcctttg atcccttgaa aatcagcact
    74881 gatttcatca gcaccgtcta ttgtcaaatc gactacgggg acttcatcaa tactctttaa
    74941 cggtattcct aatttttccg cttgttcttt tgtcgcattt gaagtagtaa ctcctgtgat
    75001 acgtaaacct tcttcacgca tacggcgacc aatctcttct accatataat aggcggttga
    75061 accggtcccg agacctacga tcatcccgtc ctttacgaat ttcgcggctt caatgccagc
    75121 taattgtttt aaattcatct ttgcgtccac ctttttttag tttgagcatc gtgctttcct
    75181 cactatttta gcgtgattta cggtaaaaag ataccattct tttttattcc aataaagctt
    75241 tttcaaaaaa aattgcatct cttgttgaca tgttttttca ttcgtgatat tctaacaaag
    75301 gtgctttatc tacaggtttc tgtgaagaaa cgtgctgact gtttatcaaa gcatatttga
    75361 taaggatgca gacattgcat ttatttttta tcacaaaaaa agaaccacct ggatgtgtgg
    75421 aactagacgc aaatcgtggg aaggaggaaa caaggaatgc ctacaattaa ccaattagta
    75481 cgtaaacctc gtaaatcaaa ggtggaaaaa tctaattcac ctgctttgaa caaaggatat
    75541 aacagcttta agaaaactca aactaacgta aactcacctc aaaaacgtgg ggtagcaact
    75601 cgtgtgggta caatgacacc taaaaaaccg aactcagctt tacgtaaata tgcacgtgtt
    75661 cgtttgtcaa acttaattga agttactgct tatatcccag ggattggtca taacttacaa
    75721 gaacacagtg ttgtattatt acgcggtggt cgtgtaaaag acttaccagg ggtacgttac
    75781 catatcgttc gcggtgcttt agatactgct ggtgttaacg accgtaaaca aagccgttct
    75841 aaatacggaa ctaaaaaacc taaagcttaa ttttaattat taaacaaatg aaacttgctt
    75901 ctactatata gaagtaatta ctatgaacaa tatttcggaa ggaggagtta cggatgccac
    75961 gtaaaggtcc tgttgcaaaa cgtgatgttt taccagatcc tatttataac tcaaaattag
    76021 taactcgctt gatcaaccgt gtaatggttg acggaaaacg cgggattgca gctaacatta
    76081 tctataatgc ctttgacttg atcaaagaat ctacaggtaa cgatccatta gaagtgtttg
    76141 aacaagcaat gaaaaacgtt caacctgttc ttgaagttaa agcacgtcgt gttgggggtt
    76201 caaactacca agtaccagtt gaagttcgcc ctgaacgccg cgttactcta ggtcttcgtt
    76261 gggtagtaaa ctacgctcgc ttgcgcggtg aacacacaat ggaagaacgc ttagcaaaag
    76321 aaatcatgga tgctgcaaac aacacaggtg cttctgttaa aaaacgtgaa gacactcata
    76381 aaatggctga tgccaaccgt gcgtttgctc actatcgctg gtaagattct ctctgtttgg
    76441 tcggctttta taagtcgacc tcagatagtg tccttatttt tagcaactag atagtaaaga
    76501 gaggagaaac aaggagaatg accagagaat tttctttaca aaacactcgt aatatcggta
    76561 tcatggccca catcgatgct ggtaaaacga ctgctactga acgtatcttg tactatactg
    76621 gtaaaatcca taaaattggt gaaactcatg aaggagcttc acaaatggac tggatggaac
    76681 aagaacaaga acgtggtatt acgatcactt ctgctgctac tacagcacaa tggaagggtc
    76741 accgaatcaa catcatcgac acaccaggac acgtagactt cacagttgaa gttgaacgtt
    76801 cattgcgtgt acttgatggt gctgtaactg ttcttgatgc acaatcaggt gttgaacctc
    76861 aaactgaaac agtttggcgt caagcaacaa cttatggtgt acctcgtatc gtatttgcaa
    76921 acaaaatgga caagatcggt gctgacttct tatattcagt atcaacttta catgaccgtt
    76981 tgcaagcaaa cgctcaccca atccaattac caattggtgc ggaagacgac ttcacaggaa
    77041 ttatcgacct tgtaacaatg aaggctgaaa tgtatacaaa cgatcttgga acagagatcg
    77101 aagaaacaga aattcctgaa gaataccgcg aattagctga agaatggcgc gaaaaattgg
    77161 ttgaagctgt agctgaaact gatgaagaac ttactttgaa atacttagaa ggtgaagaaa
    77221 tcactgaagc tgagttaaaa gaaggtatcc gccgcgcaac agttaacgta gaattctacc
    77281 cagtactatg tggttcagca tttaagaaca aaggggttca attactattg gacgctgttc
    77341 ttgattactt gccatcaccg ttagatatcc cagctatcaa agggattgac cctaaaactg
    77401 atgaagaagt agaacgtcct gcagacgaca gcgctccttt ctcagcttta gcatttaaag
    77461 ttatgactga cccattcgta ggtcgtttaa ctttcttccg tgtgtactct ggtgttttac
    77521 aatcaggttc atacgtacaa aacgcaacaa aaggcaaacg tgaacgtgtt ggtcgtatcc
    77581 tacagatgca tgccaactct cgttcagaaa tctctgaagt ttacgctgga gatatcgctg
    77641 ctgctgttgg tttgaaagac acaactactg gggacacact atgtgatgaa aagagtctag
    77701 ttattcttga atccatggaa ttcccagaac cagttatcca agtcgctatt gaacctaaat
    77761 caaaagctga ccaagataaa atgggtgtgg ctttacaaaa actatctgaa gaagatccta
    77821 ctttccgtgc tgaaacaaac gtggaaacag gcgaaacaat catcgctggt atgggtgaac
    77881 ttcacttaga tatcatcgtt gaccgtatgc gtcgtgaatt caaagttgaa gctaacgtag
    77941 gtgcgcctca agtatcttac cgtgaaactt tccgtgctgg cactcaagcc gaaggtaagt
    78001 ttgtacgtca gtctggtggt aaaggtcaat acggtcacgt atggattgaa tttacaccaa
    78061 atgaagaagg tgctggtttc gaatttgaaa atgcgatcgt cggtggggtt gttcctcgtg
    78121 aatacattcc agctgttgaa actggtttga aagatgctat ggaaaatggt gtcttagctg
    78181 gctatccatt agtagatatc aaagctaaac tttacgatgg ttcataccat gatgtcgatt
    78241 caaatgaaac tgccttccgt gttgctgctt ctatggcatt acgtgcagca gctaagaaag
    78301 ctaatccagt aatccttgaa ccaatcatgg ctgtagaagt tgttatccct gaagattatt
    78361 taggtgatgt tatgggtcac gtaactgctc gtcgtggacg tgttgaaggt atggaagctc
    78421 gcgctggcgg ccaacaagtt gttcgtgcga tggttccact tgctgaaatg tttggttatg
    78481 ctacaactct tcgttctgct actcaaggtc gtggtacatt tacaatgaca tttgaccact
    78541 acgaagatgt accaaaatca gtacaagaag aaatcattaa gaaaaacggc ggaaaagctg
    78601 aataatttct taaaaccgga tagtcctgca aagagattat ccaaagagac tatattttta
    78661 atatagtttc gtgtaaaata attatcgatg atatgggcat gtggtgtacc ttgtgtacat
    78721 catataccta tcagacaaat aaaaaatata ttgtattttt aggaggacat tttaaaatgg
    78781 caaaagaaaa atttgaccgt tctaaaccac acgttaacat tggtactatc ggacacgttg
    78841 accatggtaa aactacatta acagctgcaa tcacaactgt actatctaag aaaaacggcg
    78901 gccaagctat ggcttacgat caaatcgatg gtgctccaga agaacgcgaa cgcggaatca
    78961 caatctcaac agctcacgtt gagtatgaaa cagatacacg ccactacgct cacgttgact
    79021 gcccaggaca cgcggattac gttaaaaaca tgatcactgg tgctgctcaa atggacggag
    79081 ctatcttggt agtttctgct gctgacggcc caatgcctca aactcgtgaa cacatcctat
    79141 tgtctcgtca agttggtgtt ccttacatcg ttgtattctt gaacaaagta gacatggttg
    79201 atgacgaaga attactagaa ttagttgaaa tggaagttcg tgacctatta acagaatacg
    79261 aattccctgg tgacgatgtt cctgtagttg ctggatcagc tttgaaagct ctagaaggcg
    79321 acgcttcata cgaagaaaaa attcttgaat taatggctgc agttgacgaa tacatcccaa
    79381 ctccagaacg tgacaacgac aaaccattca tgatgccagt tgaagacgtg ttctcaatta
    79441 ctggacgtgg tactgttgct acaggtcgtg ttgaacgtgg acaagttcgc gttggtgacg
    79501 aagttgaagt tgttggtatt gctgaagaaa cttcaaaaac aacagttact ggtgttgaaa
    79561 tgttccgtaa attgttagac tacgctgaag ctggagacaa cattggtgct ttactacgtg
    79621 gtgttgcacg tgaagacatc caacgtggac aagttttagc taaaccaggt acaatcacac
    79681 ctcatacaaa attctctgca gaagtatacg tgttgacaaa agaagaaggt ggacgtcata
    79741 ctccattctt cactaactac cgtccacaat tctacttccg tacaactgac gtaacaggtg
    79801 ttgttgaatt accagaagga actgaaatgg ttatgcctgg cgacaacgta acaatggaag
    79861 ttgaattaat ccacccaatc gctatcgaaa acggtactaa attctcaatc cgtgaaggcg
    79921 gacgtacagt tggtgccggt gtcgttacag aaatcaaagc ttaattcact taagttttaa
    79981 agtaaagcaa catttattcg gacgtaatcg ctcggaacga aggtcttagg atctttgttc
    80041 cgagcttttt tatttattgc aggatcagga aaaaagacag gagtattata aatagaagaa
    80101 acacgaaata aaagagtcag tattttctaa atgaaacttt atgaaaacaa aatgtttttt
    80161 acaagaaaaa gccttgcttt gtgagtttat atatattata atgacaaagg tgcgtttact
    80221 ttagtgaatt gcacagacgt aatcagaatg atttcgtcgg ctaagaaacg agaggttgcg
    80281 acacgcccgg acgctttgcc acgaacgagc gtgacggaaa ttttcgtgga gctatgtcta
    80341 cttttcaaaa taggcgaagg agggaacaag atggcaaaac aaaaaattcg tatccgttta
    80401 aaagcgtatg aacaccgtat tttagatcaa tcagcggata aaatcgtgga aactgcaaaa
    80461 agaactggag ctagcgtgtc tggtccaatt ccattaccaa ctgaacgcag tctttacaca
    80521 atcatccgtg cgactcataa atacaaagat tcacgcgaac aattcgaaat gcgtacacac
    80581 aaacgtctta tcgacattgt gaacccaaca ccaaaaactg ttgatgcttt gatgaagcta
    80641 gacttaccat ctggtgtaaa catcgaaatc aaattataaa actaaaacaa aaataatgga
    80701 ggtgtactca tgaccaaagg aatcttaggg aaaaaagtgg gaatgacaca aatcttcact
    80761 gaatctggtg aattaattcc agtaactgtt gtagaagcta ctccaaacgt agtactacaa
    80821 gtgaaaacaa tggaaacgga tggctacgaa gctattcaag ttggttacca agatatgcgt
    80881 gaagttttat caaacaaacc tgcgaaaggt catgttgcaa aagcaaacac ggctcctaag
    80941 cgcttcattc gtgaattcaa aaatgttgag ctaggagaat atgaagtagg aaaagaaatt
    81001 acagtagatg ttttccaagc aggagacatt attgatgtaa caggtactac gaaaggtaaa
    81061 ggattccaag gggttatcaa acgccacgga caaagccgcg gaccaatggc tcacggttct
    81121 cgttaccatc gtcgtcctgg gtcaatgggt ccagttgcac ctaaccgtgt atttaaaaac
    81181 aaacgtcttg ctggccgtat gggtggcaac cgcgtaacta tccaaaactt ggaagttgta
    81241 cgtgtcgatg cagaaagaaa tgttatcttg atcaaaggta atgttcctgg agctaaaaaa
    81301 tctttaatta ctatcaaatc agctgtgaaa gctaaataat tgtaggaaga aaggaggaac
    81361 taaggaatgc cgaatgtagc attattcaaa caagatggaa gccaaaacgg tgaaatcaca
    81421 cttaacgaag aaatcttcgg aattgaacct aatgaaagtg ttgtttacga tgcaatcgta
    81481 atgcaacgtg cgtcattaag acaaggaaca cacgcggtta aaaaccgtag cgctgttcgc
    81541 ggcggcggac gtaaaccatg gcgtcaaaaa ggaacaggtc gtgcacgtca aggatctatc
    81601 cgctcaccac aatggcgtgg aggtggcgta gtctttggac caacaccacg ttcttacagc
    81661 tacaaacttc ctaaaaaagt tcgtcgttta gcaatgaaat ctgtattgtc agaaaaagtt
    81721 gctgaaaaca acttggtagc aatcgaaggc ttgaacttcg acgcaccaaa aacaaaagaa
    81781 ttcaaacaag ttcttgctaa cttatctatc gactctaaag tattggttgt tctagaaaaa
    81841 ggtaacgact ttgctgcttt atcagcacgt aacttaccaa acgtttctgt agtaacttct
    81901 gataatgtga gtgtgctaga cgttgtatca aatacaaaag tgttagcaac acaaactgct
    81961 cttactcaaa ttgaggaggt gcttgcataa tgaacttact agacgtaatc aaacgcccag
    82021 tgatcactga aaaatcaatg cttgccatgg atgacaagaa atacactttt gaagtggaca
    82081 ctcgcgcaaa caaaacttta gtaaaacaag cggtagaagc tgcttttgac gttaaagtca
    82141 aaaacgtgaa catcgtgaac gtacgtgcga aattcaaacg tatgggcaaa tatgcaggat
    82201 atacaaaaaa acgtcgcaaa gcgatcgtta cattgactga agattcaaaa gaaatccaat
    82261 tattcgaagc tgctgaataa gcactaatag ataactcatt aaattaggag ggaaaaacgt
    82321 ggcgattaaa aagtacaaac ctacctcaaa tggtcgtcgt aacatgacag gttctgattt
    82381 tgctgaaatc acaacatcaa cacctgaaaa atcattgtta caaccattga aaaaccatgc
    82441 tggacgtaac aacaacggcc gcatcactgt acgtcatcaa ggtggcggac acaaacgcca
    82501 atatcgcgtg atcgacttca aacgtaacaa agataacgtt gttgcaactg ttcaaacgat
    82561 cgaatatgat ccaaaccgtt cagctaacat cgcgttagta cactatgaag atggagtgaa
    82621 agcttacatc ttagcaccta aaggactaga agtgggtatg aaacttgttt caggtccaga
    82681 agcagatatc aaagtcggaa atgctcttcc attagaaaac attccagttg gtactgttat
    82741 ccacaacatt gaaatgaaac ctggtaaagg tggacaattg atccgttctg ctggtacaag
    82801 tgctcaagta cttggtaaag aaggcaaata cgtattgatc cgcttgaact ctggtgaagt
    82861 tcgtatgatc ttagctgcat gccgtgctac aatcggttct gtaggtaacg aacaacacga
    82921 attaatcaac atcggtaaag ctggacgctc tcgttggatg cgtaaacgcc caactgtacg
    82981 tggtagcgta atgaacccta atgatcaccc acacggtggt ggtgaaggta aagctcctat
    83041 cggacgtaaa gcaccagttt ctccttgggg tcaaccagct cttggttaca aaactcgtaa
    83101 caaaaaagct aaatcagaca aacttattgt tcgtcgtcgc aaaaataaat aattactttg
    83161 accatgtgtc gcacattttg agaggaggtt caccatgggt cgtagtttaa agaaaggacc
    83221 ttttgtcgat gagcatttaa tgaaaaaagt tgaagctcaa gcaggggttg aaaagaaaaa
    83281 agttatcaaa acttggtcac gccgttcaac agttttccca agttttgttg gatataccat
    83341 cgcagtttat gatggacgta aacatgtacc agtttacatc caagaagaca tggtaggaca
    83401 caaattaggt gaatttgcac ctacaagaac ttatcgtgga catgctgctg acgataagaa
    83461 aacgaaacgc taatttgagg ggaggataaa tccaaatggc agaacaaatc acatcagcta
    83521 aagcaactgc aaaaacagtt cgcacttcac ctcgaaaagc ccgtttagta attgacctta
    83581 tcagaggtaa aagtgtcgca gatgcaattt caatcttgaa attcacacca aacaaatctg
    83641 ctggaatcat tgaaaaagta ttgatgtcag cagttgcgaa tgcagaaaat aactttgact
    83701 tagatgttga aaacttggta gtaagcgaag cttttgttaa cgaaggacct acaatgaaac
    83761 gtttccgtcc tcgtgcaaaa ggatcagctt ctccaatcaa caaacgtaca agtcatatca
    83821 cggtagtcgt atcagaaaaa taaggaggga taatcagtgg gtcaaaaagt acatccaatt
    83881 ggaatgcgtg taggcatcat ccgcgactgg gatgctaaat ggtatgctga aaaagagtat
    83941 gctgagttct tacacgaaga tttaagaatc cgtaaattta tttcaactaa acttgctgat
    84001 gctgctgtat caacagttga aatcgaacgc gcaacaggtc gcgtaaacat ttcaattcac
    84061 acagctaaac caggtatggt tattggtaaa ggcggttctg aagtcgaaag cctaagaaaa
    84121 gaattaaaca aattaactgg taaaagaatc catatcaata tcgtggaaat caaaaaacca
    84181 gatctagatg caaaattagt aggtgaagga atcgcacgtc aattagaaaa ccgtgttgct
    84241 ttccgtcgcg ctcaaaaaca agccatccaa cgctcaatgc gtgctggcgc tcaagggatc
    84301 aaaactcaag tatctggtcg tctaaacgga gcagatatcg ctcgttcaga aggatactca
    84361 gaaggaactg ttcctcttca tactttacgt gcggacattg attacgcatg ggaagaagca
    84421 gatacaacat acggaaaact aggagttaaa gtgtggatct atcgtggaga aattcttcca
    84481 actaaaaaaa acactgagaa aggagggaaa taatcatgtt agtacctaaa cgtgtaaaac
    84541 accgtcgtga attccgcgga aaaatgcgcg gggaagctaa aggcggaaaa gaagtagcat
    84601 tcggtgaata cggtttgcaa gctgttgatt cacattggat cacaaaccgc caaatcgaag
    84661 ctgctcgtat cgcaatgact cgttacatga aacgtggtgg gaaagtatgg attaaaattt
    84721 tccctcacaa atcttatact gccaaagcaa ttggggtacg tatgggttct ggtaaagggg
    84781 cacctgaagg atgggttgca ccagtaaaac gtggtaaaat catgtttgaa atcgcaggcg
    84841 ttcctgaaga agtagctcgt gaagcgttac gtctagcttc tcacaaatta ccaatgaaaa
    84901 ctaagatcgt aaaacgtgag gaaatgggtg gtgaatcgaa tgaaggttaa agaaatcaga
    84961 gaattaacca ctgccgaaat gcttgatcaa gaaaaacaat taaaagaaga attgtttaat
    85021 cttagattcc aattagcaac aggtcaatta gaaaacactg cacgtattaa agaagtacgt
    85081 aaatcgattg cacgcatcaa aactgtgttg cgtgaacaag ctaagtaatt atggaaggag
    85141 gccattaagc gtatgactga aggaagaaat caacgcaaag tttatcaagg tcgcgtggtt
    85201 tcagacaaaa tggataaaac aattaccgtt gctgtcgaaa caaagaaaaa ccaccctatc
    85261 tatggcaaac gcatgaacta ttctaagaaa tacaaagtgc atgatgaaaa caacacagca
    85321 aaagttggcg acatcgtgag aattatggaa actcgtccat tatcagctac aaaacgtttc
    85381 cgtttgttgg aagtagtcga agaagcagtt attatctaat aacacgagat tttcctatat
    85441 agattgaatg caaaatctga aaggaggata cacaacgtga tccaagcaga aagtcgttta
    85501 aaagtcgctg ataactcagg cgcacgtgaa attttgacga tcaaagtcct tggtggatct
    85561 ggtcgtaaaa ctgctaacat cggtgatgtg atcgttgcta cggttaaaca agcaacgcca
    85621 ggtggggttg tcaaaaaagg tgacgtcgtt aaagccgtta tcgttcgaac aaaatcagga
    85681 gctcgtcgta cagacggttc atatattaaa tttgatgaaa atgctgcagt gattattcgt
    85741 gatgataaaa gccctcgtgg aacacgtatc tttgggccag ttgcacgtga actacgtgaa
    85801 aacaacttca tgaagatcgt ttcactagca ccagaagttt tataatcttg agacacttat
    85861 caaaggaggt gcgaaacagt tatgtttgtt aaaaaaggcg ataaagttaa agttatcact
    85921 ggaaaagaca aaaacaaaga aggcgttgta ctagcagcgt tcccgaaaaa agataaagtt
    85981 attgtcgaag gtgtaaatgt cgttaaaaaa caccaaaaac cttctcaagc tgctcctcaa
    86041 ggtggaattg ttgagatgga agcgccaatt catgtttcta acgtgatggt ggttgactct
    86101 actggtgtag ctggccgagt tggctacaaa gaagtagacg gcaaaaaagt ccgtgtttct
    86161 aaaaaaaccg gtgaagtttt agataaataa aagtttaagg aaggagggtc ttactgaatg
    86221 aaccgcctaa aagaaaaata tcttaaagaa gtaactccat cattgatgga aaaatttaac
    86281 tatagctctg ttatgcaaac acctaaagtt gaaaaaatcg tcatcaacat gggtgttggt
    86341 gatgcggtat caaacgctaa aaacttagat aaagcagttg aagaattaac tttgatcact
    86401 ggtcaaaaac caatgattac aaaagctaaa aaatcaatcg ctggattccg tttacgtgaa
    86461 ggaatgccaa ttggtgcaaa agttacattg cgcggagaaa gaatgtacga attcttagac
    86521 aaattggttt ctgtttcact accacgtgta cgtgacttcc atggtgtaag taaaaaagcc
    86581 tttgacggtc gtggaaacta tactttaggt attaaagaac aattgatctt cccagaagta
    86641 gattatgatt tagtagataa agtacgtggt atggacatcg ttattgtaac aacagcaaac
    86701 tctgatgaag aatctcgtga gttgttgaca caattaggca tgccattcca aaaataatta
    86761 aaggaggcga actacgtggc taaaaaatca atgattgcta aaaacaaacg tcctgcaaaa
    86821 ttttcaacac aagcttatac tcgttgcgaa cgttgcggac gtccacattc agtttatcgt
    86881 aagttccatc tttgccgtat ttgcttccgc gaacttgcct ataaaggtca aattcccggc
    86941 gtgaagaaag ctagctggta aaaagtaaat tcgcataaga aggaggtaaa agatcaatgg
    87001 tcatgacaga tccaattgca gattttctaa ctcgtatccg taacgcaaac atggttaaac
    87061 atgagtcttt agaagtacct gcatcaaaaa ttaaacgtga catcgctgaa atcttgaaac
    87121 gtgaaggttt cgttcgtgat gttgaataca tcgaagatga caaacaaggc gtgatccgtg
    87181 ttttcctaaa atttggtaaa aacggcgaac gtgttatcac aaacttaaaa cgtatctcta
    87241 aaccaggttt gcgtgcttat gtcaaagctg acgaagtacc aaaagtattg aacggtttag
    87301 gtatcgcaat catttcgact tctgaaggtg ttgtcactga taaagaagct cgtgaaaaaa
    87361 atatcggcgg cgaagttatc gcttacgtat ggtaatcaag tagaatctac aaggaggtgt
    87421 ctttaagtga gccgtatcgg aaataaagtc gttgttatcc ctgaaggagt aacagttact
    87481 caagacggaa acaacgttac agttaaagga ccaaaaggtg aattgactcg tactttttca
    87541 gcagatatca aaatgaatat cgaaggaaac gaagtaactt tcactcgtcc aaacgacagt
    87601 aaagaaatga aaacaattca tggaacaact cgtgcgaact tcaacaacat ggttgtaggt
    87661 gtcagcgaag gtttccaaaa agcgttagaa ctaatcgggg ttgggtaccg tgcacaatta
    87721 caaggatcaa aacttgtatt gaacgttggt tactcacatc cagtagaaat cacaccacca
    87781 gctggagtaa ctgttgaagt accagctaac acacaagtaa ttgttaaagg tgcgaacaaa
    87841 gaagaagttg gcgaattagc tgcaaacatt cgtggaactc gtcctccaga accttataaa
    87901 ggtaaaggta ttcgttatgt tggcgaatat gtacgccgta aagaaggtaa aactggtaaa
    87961 taatcgttta gaaaaattgc atagaagcca gcagcaggaa aaaggcattt ggctgaagtt
    88021 taccttcaaa aaaatacatt tttacttgtg ccgcagcttc tatttttcga aacgtattta
    88081 ctgcttgaaa tagtagtaca ataaaaataa agaggtgaca attgtgatta caaaaccaga
    88141 taagaacaaa acacgtcaaa agagacatcg tcgtgtacgt aacaatatct ctggtactgc
    88201 tgagcgccca cgcttgaacg tttttcgttc taacaaaaac atctacgctc aaattattga
    88261 tgacgtagcg ggtgtaacgc tagcaagtgc ctctaccttg gataaagaaa tttcaggtgg
    88321 aacaaaaaca gaagcagcac aagctgtcgg taaactagtt gctgaacgtg cagctgaaaa
    88381 aggcattaaa gtagtagtct ttgaccgtgg tggatacctt taccatggcc gtgtgcaagc
    88441 tttagctgaa gctgctcgcg aaaatggact agaattttag aagaaggagg aataccattc
    88501 atggtttata tcgatccaaa acatttggaa ttagaagacc gcgttgttgc aattaaccgt
    88561 gtaacaaaag ttgttaaagg tggacgtcgt ctacgttttg ctgctttagt tgttgtcggt
    88621 gacaaaaacg gacacgtagg gtttggtaca ggtaaagctc aagaagttcc tgaagcaatc
    88681 cgtaaagcaa ttgaagatgc gaagaaaaac ttagttgaag tacctatggt tggttcaaca
    88741 atcccacacg aagtaatcgg tgcttttagt ggtggtagaa tcctaatgaa acctgctgta
    88801 gaaggttctg gggttgctgc tggtggacca gttcgtgccg tattagaatt agctggggta
    88861 gcagatatca cttctaaatc tttaggctca aatacaccaa tcaacgttgt tcgcgcaaca
    88921 gttgaaggtt tgaaacaatt gaaacgtgca gaagaagttg cagaacttcg cggcaaatca
    88981 gttgaagaat taatcggata aggaggacaa caataatggc tgaattaaaa gttactttaa
    89041 aacgcagtat tatcggacgt cctcaaaacc aacgcgatac agttaaagcg ttaggtctta
    89101 caaagattaa cagttcagtg gttaagcctt ctaatgaagc aattaaaggc atgatcaata
    89161 ctgtttctca cctagtggac gttgaagaaa tttaatttag aaaaatcata actagaacat
    89221 taaggaggtg ccaaacctat gaaacttcat gaattaaaac ctgctgaagg ttcacgccaa
    89281 gtacgtaacc gtgttggtcg tggtacttca tctggtaacg gaaaaactgc cggacgcgga
    89341 caaaaaggac aaaaagctcg ttcaggcggc ggtgtgcgtt taggatttga agggggacaa
    89401 acaccattgt tccgtcgttt accaaaacga ggattcacaa acgtgaaccg taaagactat
    89461 gcagtcatta atcttgatgt attgaaccgc tttgaagacg gagctgaagt aactcctgtt
    89521 gctttagttg aagctggaat cgtgaaaaac gaaaaagctg gaatcaaagt attagctaat
    89581 ggggaactaa acaagaaatt aactgtgaaa gcagcaaaat tctctaaagc agctcaagaa
    89641 gcaattgaag cggctggtgg atcaatcgag gtgatctaat gtttagacta ttaaaagatg
    89701 cttttaaagt aaaagacatt agatcgaaaa tcctatttac tgtatttgtg ttgtttgtat
    89761 ttcgcctagg tgcacatatt actgtaccag gcgtaaatgc tgcttcattg caaaaccttt
    89821 cttctttgcc atttttcaac atgttgaact tggtaagtgg tagtgcgatg caaaactttt
    89881 ccattttctc aatgggggtt tcaccataca ttactgcttc tatcgtcatt cagcttcttc
    89941 aaatggacat cgtgcctcga tttgttgaat ggtcgaaaca aggggaagtt ggtcgtaaaa
    90001 aactgaatca agcgactcga tatctgacat tagtattggc atttatccaa tctgtcggta
    90061 tcactgctgg attcaatcaa tacgctcaat taggtttcgt gaataatcct aacgtagcaa
    90121 cttacgtaac gatcggtttg attctaacag ccggtacgat gtttgttact tggctaggag
    90181 aacagatcac agaaaaaggt atcggaaatg gcgtatcaat gatcattttt gcaggtatca
    90241 tttctagatt gcctgaaagt atcagagaaa tttatgaaga ttactttata aatattgatc
    90301 cttcagacat atggaaatct gctatttttg tggccatttt actagtagcg gtcttgttga
    90361 tcgtgacatt cgtaacattt ttccaacaag ctgaacgcaa gatcccgatc caatatacga
    90421 aacgcgtagc tggcgcgcct acaagcagct atctgccgtt gaaagtaaat gcagctggag
    90481 ttattccagt tatctttgct agttcgttta ttgctacgcc aaatgctgtt ttacaagcat
    90541 tgagcggtag attcggttct gaaaactggt acaaagtgat gatggagatc tttaactata
    90601 atacagcacc tggtgctacg atttatacga ttctgatcgt tgccttcact ttcttctacg
    90661 cctttgttca agtaaaccct gagaaattag cggaaaactt gcaaaagcaa ggaagttata
    90721 taccaagcgt acgccctggt aaggggactg aagagtatgt ctcaagatta ctgatgcgtt
    90781 taagtacagt tggtgcagtc ttccttggtt tagtggcatt gcttccaatc attgctcaaa
    90841 tggtttggga tcttccacaa tcaatcggat taggcggtac aagcttactg atcgttatcg
    90901 gtgtagcttt agaaactgcg aaacagttag aaggcttaat gctgaaacgt aaatatgttg
    90961 gattcattga gtaagaaagc gtgttgaggt ttcccctcaa cacacgttct cacacattta
    91021 ttaggaggga aaacaatgaa cctcatttta atggggctgc caggcgcagg taaaggaaca
    91081 caagctgaaa aaatcattga tgcttatggt attccgcaca tctcaacagg agatatgttc
    91141 cgcgcagcga tgcaaaacga aacagctctt ggcctggaag caaagtctta tatggataaa
    91201 ggtgcattag ttcctgatga agtaacaaat ggaatcgtga aagagcgact agctgaacca
    91261 gatacagaga aaggcttctt attagatggc tttccacgta cgctagatca agcaaaagct
    91321 ctggacgcaa tgctaaaaga tttgaataaa aaaattgatg ctgtcatcga tatccatgtt
    91381 ggtgaagaaa tcttggtcga acgtctagcc ggccgattta tctgcagcaa ttgtggagca
    91441 acttaccata aaattttcaa ccctacaaaa gttgaagaca catgtgatcg ttgcggtggg
    91501 catgaattct atcaaagaga agatgataaa cctgagacgg ttaaaaatcg tctggctatc
    91561 aacatcaaaa atagtgaacc aatcttggct tattataaag aacaaggttt gctaaacact
    91621 attgatggtg ctcgtgaaat cgatgctgta tttgcagatg tacaaaaaat cattgaaaaa
    91681 tagcacgatt gcccgtagcc gacttgcatt atgcagttac tcttgtcaag ttcaataaat
    91741 tatgatagaa tatgtcagtc cgacttctag agaatcctct agatgatcca ttttgagaat
    91801 tttgaggtgg gaactgcagt ttccgccatt ttatcgtgtg aaaatgcgaa acaagtacaa
    91861 ggaggtactg tgcgtggcaa aagaagatat gattgaagtc gaaggtacag tcgtcgaaac
    91921 tttgccgaat gcaatgttta aagttgaact agaaaacgga caccaagttc ttgctactgt
    91981 ttcaggtaaa attcgtatgc attacatccg tatcttacct ggtgacaaag taacggttga
    92041 attgtctcca tacgatctta accgtggccg tattacttat cgctttaaat aattgtactc
    92101 cgtaaaacta caggaggtat tatcatgaaa gtaagaccat cagtaaaacc aatgtgcgaa
    92161 cattgtaaag taattcgtcg taaaggacgc gttatggtga tttgcccagc aaatccaaaa
    92221 cataaacaac gtcaaggata attggaggtg ctataaatat ggctcgtatt gcaggagtag
    92281 atattcctcg tgataaacgt gtagtagttt cacttactta tatctatggt attggtaaca
    92341 ctactgctaa aaagatctta gctgatgctg gtgtatctga agatgttcgc gttcgtgaat
    92401 taacgaatga acaaacagat gctatccgtg cagaagtaga taaattaaaa gttgaagggg
    92461 atcttcgtcg tgaagtgaac ttgaacatca aacgtttgat ggaaatcggt tcttaccgtg
    92521 gaatccgtca tcgccgtgga ttgcctgttc gtggacaaaa cacgaaaaac aatgcacgta
    92581 ctcgtaaagg cccagctcgc gctatcgctg gcaagaaaaa ataattctta agtgaaggag
    92641 gttaaaactt catggcagca aaaaaagtga atcgtaaacg ccgtgtgaag aagaatatag
    92701 aagcaggtat tgcacatatc cattctacat tcaataacac tatcatcatg atcactgata
    92761 ctcacggaaa tgcattagcg tggtcatcag ctggttcatt aggtttcaaa ggaagcaaaa
    92821 aatcaactcc ttttgccgct caaatggcag cagaaactgc tacaaaagca gcaatggaac
    92881 acggattaaa gactgttgaa gtaacagtta aaggacctgg ttctggacgt gaagcagcaa
    92941 ttcgttcatt acaagctaca ggtttagaag tgactgcaat tcgtgacgtg actccagttc
    93001 ctcataatgg tgcccgccct ccaaaacgcc gtcgtgttta atgagtgccg ctcattttga
    93061 gttttatcag taacgtcatt gaacaagaca cttttcgttt tgaaaggggt aaagataaga
    93121 atgattgagt tcgaaaaacc aagaatcgca aaaattgatg aagaaaaaga ttatggcaag
    93181 ttcatcgttg aacctcttga aagagggtat gggactactt tagggaattc cctacgtcgt
    93241 attttattat cttctttgcc aggtgctgcc atcactaata ttcaaatcga tggtgtactg
    93301 catgaattct ctaccatcaa aggtgttaga gaagacgtca ctcaaatcat cttgaacatt
    93361 aaaggcttag cgctgaaaat gtatggccaa gaagaaaaaa cccttgaaat cgatattacc
    93421 ggacctgcta cagtaactgc tggcgatatt atcgttgata gtgatgtaga aattcttaac
    93481 aaagatatgt acatctgtag tgtctctgaa ggagctacgt tccatgctcg cttaacagtg
    93541 aaacctggac gtggttatgt tcaagcagat gaaaataaaa aagaagatat gccaattggc
    93601 gttcttccag tcgattcaat ctacacacct gttcgtcgtg tgaactatca agttgagaac
    93661 acacgtgttg gacaccgtga agattttgat aaattgacga tggagatttg gacagatggt
    93721 tcaattgaac cgttggaagc aatgagtttg tctgcgaaaa tcatgactga acatttggat
    93781 atttttgtga acttaactga tgaagcaaaa aatgctgaaa tcatggtcga aaaagaagaa
    93841 acacaaaaag aaaaaatgtt ggaaatgacg atcgaagagt tagacttgtc tgttcgctct
    93901 tacaattgtt tgaaacgtgc aggaatcaat actgtacaag aacttacaaa taaatctgag
    93961 cctgaaatga tcaaagtccg taacttagga cgtaaatcat tagaagaagt taaagcaaaa
    94021 ttgcacgact taggtttagg tttacgtaaa gaagattaaa accttttacg aaggagggaa
    94081 accaacgtga gttatcgtaa actaggacgc acatctagcc aacgtaaagc gatgttgcgt
    94141 gatttaacaa ctgatttatt aatcaacgaa cgtattgtga ctactgaagc tcgtgcgaaa
    94201 gaagttcgct caactgcaga aaaaatgatc actttaggta aacgcggaga tttgcatgca
    94261 cgtcgtcaag ctgcggcttt cgtacgtaat gaagttgcca gcgtgcgtga agaaaatgag
    94321 gacatcgtca ttgaatcagc tttacaaaaa ctgttcaacg atattgcacc tcgttacgca
    94381 gaacgtcaag gcggatacac acgcattttg aagacagaac caagacgcgg tgatggtgca
    94441 ccaatggttg tcttagaact tgtctaaaat cacactaata acatcacttt atagatgatt
    94501 cttttagagt gttatgatgt tggaagatat ttttccgagt ctagctcagc gctgctcctc
    94561 aagcgaatat cgcttgggga gtaagcactc atcataaagt gtttttttat accttcaatt
    94621 acctgtcatg acagatatgt ttgaaggcat aaaaaaacat tttaagcgag aaataattga
    94681 aaattttgtc tttttttgat agtgttagta ggaactttag gatgaagaag gagtaatcat
    94741 ggagccaatt attgaattag aaaagataaa ttataaatac cagccagatg atcttcgccc
    94801 cgcattgaaa gatgtctcct ttacaattga taaaggtgag tggatcgcta ttattggaca
    94861 caatggttca ggaaaatcga cacttgccaa aacgatcaat ggattgcttc tgccagaatc
    94921 agggatcgtg aaagtgggaa atcaaatatt ggatgaagaa aatatttgga cgatccgaca
    94981 gatggttgga atggtttttc aaaatccaga caatcagttt gttggttcga cagtagaaga
    95041 tgacgttgct tttggtcttg aaaatcaagg gattcccaga gaagaaatgc tagtgcgtgt
    95101 gaaagatgca cttgagaaag ttagaatggc tgagtttgct tcacgcgagc ctgctcgttt
    95161 atcaggaggg caaaaacaac gtgttgccat tgccggcgtg gttgccttac gacctgatat
    95221 cattatttta gatgaagcaa ccagtatgct agatccagaa ggtcgtgaag aagtgatctc
    95281 tacgatcaag aagatcaaag aagaaagtca attgaccgtt atatctatca ctcatgatat
    95341 tgatgaagca gctaatgcca atcggatact agtgatgaga caaggcgaac ttgtccgtga
    95401 aggtacacca aaagaaattt tttctgctgg tcctgaactg atcgacttag gtctagatct
    95461 accatttcct gaaaaactga agagcgcact gaaagaacgt ggggttgatg tgcccagtga
    95521 atatatgaca gaagaaagga tggtggattg gttatggaca tccgttttga acaagtagac
    95581 ttcacttatc agccgaatac accatttgaa caaagagcct tatttgacat caatatgacg
    95641 atcaaagaaa acagttatac tgcattagta gggcataccg gaagcgggaa atcaacctta
    95701 cttcagcatt tgaatgcgct tgttaagcca acaagcggta cagtccatat tggagaacga
    95761 gatattcagc cggatacgga taataagaat ttaaagccca tccgaaaaaa agttggcatc
    95821 gtctttcagt ttccagaagc acagctattc gaagaaacgg tagcaaaaga cattgctttc
    95881 ggtcctaaaa actttggtgt cagcgaagaa gaagcactag tcctagcaaa agaaacatta
    95941 gaacaagttg ggctggatga aagctatttg gaacgttcgc catttgaact ttcaggaggg
    96001 caaatgcgta gagtagcaat cgctggtgtg cttgccatga ggccggaagt acttgtattg
    96061 gatgaaccaa cggcaggact tgatccccag ggtagaaaag aaatgatgga gatgttttgg
    96121 cgtcttcata aggaacagca gatcactatt gtattagtta cgcatttaat ggatgatgta
    96181 gccaattttg cagattatgt ctatgtgttg gaaaagggac gaatcgttaa tagcggggag
    96241 ccgcaagagg tctttcaaaa cattgaatgg ctaaaagaaa aacaactagg cgtacctact
    96301 gccaccgagt ttgcagaaga attaatggct aaagggatga actttgctac cttgcctttg
    96361 acagctgaag aattagcaga cgctatcgtc cattatgctg gaggaaatgc ccatgatgaa
    96421 taaattgata tttggtcgtt atatgcctgg agattccttg atccatcgat tggatccccg
    96481 agctaaatta ttagccagtt tttattttat cggaatcatt tttttagcaa ataattggga
    96541 aacgtacctt ttgttggcat ttttcacatt actttctgtc ttcttgtcaa aagtagattt
    96601 gaaatttttt atccgaggca ttcgcccatt gatctggcta atcctcttca ctgtagcttt
    96661 gcaggtcttg tttacaagtg gcggagaaac ttattggagt tggggtgttt tccatatcac
    96721 tgaatttggt gtgatgaacg gcctctttat tttctgtcga ttcgtgttga tcatttttat
    96781 gtcgactcta ctcacgctta ctacaccgcc tttggaatta tcggatgcga ttgagtactt
    96841 gcttcgtcca ttgaaagccg tacggttccc agtccacgaa atttcattga tgctgtcgat
    96901 tgctcttcgt tttgtgccta cattgatgga cgaaacagaa aagatcatga acgctcagcg
    96961 cgctcgcggt gttgattttg gtgaaggaaa cctagttcag aaaatgaaag cagtcattcc
    97021 tttgctgatt cctttatttg tcagcagctt caaccgtgcg gaagatcttg cgactgctat
    97081 ggaagcaaga ggctacaaag gtggggaagg aagaacgaaa tatcgtgtat tgcattggca
    97141 taatagagac acgatggtta tcattgcttt tgtattatta actgtgctac tgatcttttt
    97201 gagaggataa acgttcagtg ggccgctatc tattcttttc ttgctgatct gtttgcaaga
    97261 aaggatgcga gcagtgtatg acaagaaata ttagggtctg tggcagacta aggtcacagg
    97321 cctctttctt ttatctgaaa agaaagaacg aaaaaatttt ataaaaaaat cattattcca
    97381 gataaccttt taggaacaaa ggagagaaaa acatggtacg ttacaaagca atcattgcat
    97441 acgacggaac aaattttaat ggattccaaa aacagccaaa tgggcgtact gttcaagaag
    97501 aagtagaaaa aacattacaa aaaatggcaa atggaaaaga aatcacggtc tttggttcgg
    97561 gaaggacaga tgccggagta catgcgattg ggcaagtaat ccattttgat tatccagagg
    97621 agcgtccgtt agaacgaatg cgtttcgcgc tagataccca gtcaccagag gatattgctg
    97681 taagacaagt agaaattgtt tcagaagact tccacgcaag atatttggtc aaagaaaaga
    97741 cgtatcaatt tcgagtggat atagggaaac cacgcagtcc ttttcgacgc cattatgcaa
    97801 gttattttcc ttaccctctt gatttggaaa aaattcagcg ggcgcttcct gatttattag
    97861 ggactcacga tttcacctct ttttgtgcat caggaagctc gatagaggat aaagtacgta
    97921 cgatctacga ggctaagatg gaggtaaatg aaacaaaaga cgaattgttg tttacattta
    97981 ggggaaatgg cttcttgtat aagatgatac ggatcttagt aggaacgctt ttgaaaattg
    98041 gcaatggccg attgcctgaa gatagtatcc cagatattat tgcgaaaaaa gatcgtaatg
    98101 cagcaggacc tacagcacat cctgaaggac tttatttgta tgaagtgaaa tatgaataag
    98161 aggttgtgac agaagtggtc agcttcaaga aataagaagg aatttacgaa aattacttct
    98221 caaaattttg caaaatttcg gcttattttc gaaggagcta cttctggaac accgtgaatc
    98281 agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa caaccaaaca aaaatagctc
    98341 gtcatatttt tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgttta
    98401 aggaagaggt tgtgaaatca gcgttttgac ctgagtatta gagaaacaaa cggaaaaata
    98461 gttcctcata ttttgccgtt tgttgagcta ataccgaagg tcagctgatt gaacaccgtt
    98521 taagaaagag gtcgtgagcc tgtcatttaa ctctgagtca caagtaacaa tttcaaaaaa
    98581 cagcttctca tctttgagga atatcgtgac ttgtggacga ggcgttgata agtgaatcat
    98641 ctttttgtta cctatttttt ctgtaaccaa aaaacgatgg aacgttgttt tgattgccct
    98701 taatgaggac agtcggaaca ggtttgtcat ttttat
//
LOCUS       DAFBQH010000003        96856 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.003, whole genome shotgun sequence.
ACCESSION   DAFBQH010000003 DAFBQH010000000
VERSION     DAFBQH010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 96856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.003"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            10..588
                     /locus_tag="LK694_000203"
     CDS             10..588
                     /locus_tag="LK694_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051106.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(828..911)
                     /locus_tag="LK694_000204"
     tRNA            complement(828..911)
                     /locus_tag="LK694_000204"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(875..877),aa:Leu,seq:gag)
     gene            966..1145
                     /locus_tag="LK694_000205"
     CDS             966..1145
                     /locus_tag="LK694_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051107.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            1294..2511
                     /locus_tag="LK694_000206"
     CDS             1294..2511
                     /locus_tag="LK694_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK5051108.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2695..3531
                     /locus_tag="LK694_000207"
     CDS             2695..3531
                     /locus_tag="LK694_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5051109.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3607..4755
                     /gene="nagA"
                     /locus_tag="LK694_000208"
     CDS             3607..4755
                     /gene="nagA"
                     /locus_tag="LK694_000208"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBK5051110.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4976..6034
                     /locus_tag="LK694_000209"
     CDS             4976..6034
                     /locus_tag="LK694_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBK5051111.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6038..7072
                     /locus_tag="LK694_000210"
     CDS             6038..7072
                     /locus_tag="LK694_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBK5051112.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(7174..7890)
                     /locus_tag="LK694_000211"
     CDS             complement(7174..7890)
                     /locus_tag="LK694_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBK5051113.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(7859..8644)
                     /locus_tag="LK694_000212"
     CDS             complement(7859..8644)
                     /locus_tag="LK694_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HBK5051114.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8635..9384)
                     /locus_tag="LK694_000213"
     CDS             complement(8635..9384)
                     /locus_tag="LK694_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBK5051115.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9682..10206
                     /locus_tag="LK694_000214"
     CDS             9682..10206
                     /locus_tag="LK694_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBK5051116.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10222..11721
                     /locus_tag="LK694_000215"
     CDS             10222..11721
                     /locus_tag="LK694_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBK5051117.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11809..12411)
                     /locus_tag="LK694_000216"
     CDS             complement(11809..12411)
                     /locus_tag="LK694_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK5051118.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12515..13471
                     /locus_tag="LK694_000217"
     CDS             12515..13471
                     /locus_tag="LK694_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBK5051119.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13776..14732)
                     /locus_tag="LK694_000218"
     CDS             complement(13776..14732)
                     /locus_tag="LK694_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK5051120.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14906..15466
                     /locus_tag="LK694_000219"
     CDS             14906..15466
                     /locus_tag="LK694_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051121.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15459..16283
                     /locus_tag="LK694_000220"
     CDS             15459..16283
                     /locus_tag="LK694_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBK5051122.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16293..16910
                     /locus_tag="LK694_000221"
     CDS             16293..16910
                     /locus_tag="LK694_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBK5051123.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16928..17611
                     /locus_tag="LK694_000222"
     CDS             16928..17611
                     /locus_tag="LK694_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBK5051124.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18294..19577
                     /locus_tag="LK694_000223"
     CDS             18294..19577
                     /locus_tag="LK694_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK5051125.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            19593..20480
                     /locus_tag="LK694_000224"
     CDS             19593..20480
                     /locus_tag="LK694_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5051126.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20497..21333
                     /locus_tag="LK694_000225"
     CDS             20497..21333
                     /locus_tag="LK694_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5051127.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21336..21959
                     /locus_tag="LK694_000226"
     CDS             21336..21959
                     /locus_tag="LK694_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051128.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21956..23248
                     /locus_tag="LK694_000227"
     CDS             21956..23248
                     /locus_tag="LK694_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK5051129.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23607..23704
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23820..24401
                     /locus_tag="LK694_000228"
     CDS             23820..24401
                     /locus_tag="LK694_000228"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBK5051130.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24404..25711
                     /locus_tag="LK694_000229"
     CDS             24404..25711
                     /locus_tag="LK694_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBK5051131.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26127..26615
                     /gene="purE"
                     /locus_tag="LK694_000230"
     CDS             26127..26615
                     /gene="purE"
                     /locus_tag="LK694_000230"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBK5051132.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26608..27732
                     /gene="purK"
                     /locus_tag="LK694_000231"
     CDS             26608..27732
                     /gene="purK"
                     /locus_tag="LK694_000231"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBK5051133.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSIEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27745..29040
                     /gene="purB"
                     /locus_tag="LK694_000232"
     CDS             27745..29040
                     /gene="purB"
                     /locus_tag="LK694_000232"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBK5051134.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29220..29597)
                     /locus_tag="LK694_000233"
     CDS             complement(29220..29597)
                     /locus_tag="LK694_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051135.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29853..30581
                     /locus_tag="LK694_000234"
     CDS             29853..30581
                     /locus_tag="LK694_000234"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBK5051136.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30581..30835
                     /gene="purS"
                     /locus_tag="LK694_000235"
     CDS             30581..30835
                     /gene="purS"
                     /locus_tag="LK694_000235"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBK5051137.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30837..31508
                     /gene="purQ"
                     /locus_tag="LK694_000236"
     CDS             30837..31508
                     /gene="purQ"
                     /locus_tag="LK694_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBK5051138.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31509..33731
                     /gene="purL"
                     /locus_tag="LK694_000237"
     CDS             31509..33731
                     /gene="purL"
                     /locus_tag="LK694_000237"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBK5051139.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33716..35155
                     /locus_tag="LK694_000238"
     CDS             33716..35155
                     /locus_tag="LK694_000238"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBK5051140.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35178..36230
                     /locus_tag="LK694_000239"
     CDS             35178..36230
                     /locus_tag="LK694_000239"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBK5051141.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            36227..36805
                     /gene="purN"
                     /locus_tag="LK694_000240"
     CDS             36227..36805
                     /gene="purN"
                     /locus_tag="LK694_000240"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBK5051142.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36802..38337
                     /gene="purH"
                     /locus_tag="LK694_000241"
     CDS             36802..38337
                     /gene="purH"
                     /locus_tag="LK694_000241"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBK5051143.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38353..39627
                     /gene="purD"
                     /locus_tag="LK694_000242"
     CDS             38353..39627
                     /gene="purD"
                     /locus_tag="LK694_000242"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBK5051144.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39749..40663
                     /locus_tag="LK694_000243"
     CDS             39749..40663
                     /locus_tag="LK694_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK5051145.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40796..42361)
                     /locus_tag="LK694_000244"
     CDS             complement(40796..42361)
                     /locus_tag="LK694_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBK5051146.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42354..43805)
                     /locus_tag="LK694_000245"
     CDS             complement(42354..43805)
                     /locus_tag="LK694_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBK5051147.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTIEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43819..44835)
                     /locus_tag="LK694_000246"
     CDS             complement(43819..44835)
                     /locus_tag="LK694_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK5051148.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45055..46143
                     /locus_tag="LK694_000247"
     CDS             45055..46143
                     /locus_tag="LK694_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HBK5051149.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46197..47723
                     /locus_tag="LK694_000248"
     CDS             46197..47723
                     /locus_tag="LK694_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBK5051150.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47725..48930
                     /locus_tag="LK694_000249"
     CDS             47725..48930
                     /locus_tag="LK694_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5051151.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49187..50164
                     /gene="guaC"
                     /locus_tag="LK694_000250"
     CDS             49187..50164
                     /gene="guaC"
                     /locus_tag="LK694_000250"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBK5051152.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50428..51852
                     /locus_tag="LK694_000251"
     CDS             50428..51852
                     /locus_tag="LK694_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBK5051153.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52313..53422
                     /gene="pdhA"
                     /locus_tag="LK694_000252"
     CDS             52313..53422
                     /gene="pdhA"
                     /locus_tag="LK694_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBK5051154.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53425..54402
                     /locus_tag="LK694_000253"
     CDS             53425..54402
                     /locus_tag="LK694_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBK5051155.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            54427..56070
                     /locus_tag="LK694_000254"
     CDS             54427..56070
                     /locus_tag="LK694_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBK5051156.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            56078..57484
                     /gene="lpdA"
                     /locus_tag="LK694_000255"
     CDS             56078..57484
                     /gene="lpdA"
                     /locus_tag="LK694_000255"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBK5051157.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            57646..57921
                     /locus_tag="LK694_000256"
     CDS             57646..57921
                     /locus_tag="LK694_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBK5051158.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            57927..58694
                     /locus_tag="LK694_000257"
     CDS             57927..58694
                     /locus_tag="LK694_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBK5051159.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            58862..60691
                     /gene="typA"
                     /locus_tag="LK694_000258"
     CDS             58862..60691
                     /gene="typA"
                     /locus_tag="LK694_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBK5051160.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            60952..62724
                     /gene="recQ"
                     /locus_tag="LK694_000259"
     CDS             60952..62724
                     /gene="recQ"
                     /locus_tag="LK694_000259"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBK5051161.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            62838..63140
                     /locus_tag="LK694_000260"
     CDS             62838..63140
                     /locus_tag="LK694_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBK5051162.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            63143..64306
                     /locus_tag="LK694_000261"
     CDS             63143..64306
                     /locus_tag="LK694_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBK5051163.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            64332..67760
                     /locus_tag="LK694_000262"
     CDS             64332..67760
                     /locus_tag="LK694_000262"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBK5051164.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            67781..68905
                     /locus_tag="LK694_000263"
     CDS             67781..68905
                     /locus_tag="LK694_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051165.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            68895..69332
                     /locus_tag="LK694_000264"
     CDS             68895..69332
                     /locus_tag="LK694_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBK5051166.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            69316..69654
                     /locus_tag="LK694_000265"
     CDS             69316..69654
                     /locus_tag="LK694_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBK5051167.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            69773..70333
                     /gene="rsmD"
                     /locus_tag="LK694_000266"
     CDS             69773..70333
                     /gene="rsmD"
                     /locus_tag="LK694_000266"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBK5051168.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            70330..70821
                     /gene="coaD"
                     /locus_tag="LK694_000267"
     CDS             70330..70821
                     /gene="coaD"
                     /locus_tag="LK694_000267"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBK5051169.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            70811..71854
                     /locus_tag="LK694_000268"
     CDS             70811..71854
                     /locus_tag="LK694_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBK5051170.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            72013..72741
                     /locus_tag="LK694_000269"
     CDS             72013..72741
                     /locus_tag="LK694_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBK5051171.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            72857..73102
                     /locus_tag="LK694_000270"
     CDS             72857..73102
                     /locus_tag="LK694_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBK5051172.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            73099..73317
                     /locus_tag="LK694_000271"
     CDS             73099..73317
                     /locus_tag="LK694_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBK5051173.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            73453..73992
                     /locus_tag="LK694_000272"
     CDS             73453..73992
                     /locus_tag="LK694_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBK5051174.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            73985..76258
                     /locus_tag="LK694_000273"
     CDS             73985..76258
                     /locus_tag="LK694_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBK5051175.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            76296..77342
                     /gene="holA"
                     /locus_tag="LK694_000274"
     CDS             76296..77342
                     /gene="holA"
                     /locus_tag="LK694_000274"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBK5051176.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(77431..77685)
                     /locus_tag="LK694_000275"
     CDS             complement(77431..77685)
                     /locus_tag="LK694_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBK5051177.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(78085..78354)
                     /locus_tag="LK694_000276"
     CDS             complement(78085..78354)
                     /locus_tag="LK694_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051178.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(78824..79279)
                     /locus_tag="LK694_000277"
     CDS             complement(78824..79279)
                     /locus_tag="LK694_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBK5051179.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(79417..81345)
                     /locus_tag="LK694_000278"
     CDS             complement(79417..81345)
                     /locus_tag="LK694_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBK5051180.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(81542..82375)
                     /locus_tag="LK694_000279"
     CDS             complement(81542..82375)
                     /locus_tag="LK694_000279"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBK5051181.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(82567..83271)
                     /locus_tag="LK694_000280"
     CDS             complement(82567..83271)
                     /locus_tag="LK694_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK5051182.1"
                     /translation="MTETTLYIVRHGKPMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(83294..83920)
                     /locus_tag="LK694_000281"
     CDS             complement(83294..83920)
                     /locus_tag="LK694_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBK5051183.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            84246..86621
                     /locus_tag="LK694_000282"
     CDS             84246..86621
                     /locus_tag="LK694_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBK5051184.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(86645..87136)
                     /locus_tag="LK694_000283"
     CDS             complement(86645..87136)
                     /locus_tag="LK694_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBK5051185.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            87323..88243
                     /locus_tag="LK694_000284"
     CDS             87323..88243
                     /locus_tag="LK694_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK5051186.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            88397..88741
                     /locus_tag="LK694_000285"
     CDS             88397..88741
                     /locus_tag="LK694_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBK5051187.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(88931..89431)
                     /locus_tag="LK694_000286"
     CDS             complement(88931..89431)
                     /locus_tag="LK694_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5051188.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            89959..90042
                     /locus_tag="LK694_000287"
     tRNA            89959..90042
                     /locus_tag="LK694_000287"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:89993..89995,aa:Leu,seq:cag)
     gene            complement(90366..91004)
                     /locus_tag="LK694_000288"
     CDS             complement(90366..91004)
                     /locus_tag="LK694_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK5051189.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            91339..91773
                     /locus_tag="LK694_000289"
     CDS             91339..91773
                     /locus_tag="LK694_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK5051190.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            91796..92116
                     /locus_tag="LK694_000290"
     CDS             91796..92116
                     /locus_tag="LK694_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051191.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            92257..92448
                     /locus_tag="LK694_000291"
     CDS             92257..92448
                     /locus_tag="LK694_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051192.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(92519..92776)
                     /locus_tag="LK694_000292"
     CDS             complement(92519..92776)
                     /locus_tag="LK694_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBK5051193.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            92934..93197
                     /locus_tag="LK694_000293"
     CDS             92934..93197
                     /locus_tag="LK694_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051194.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            93221..93409
                     /locus_tag="LK694_000294"
     CDS             93221..93409
                     /locus_tag="LK694_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051195.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(93492..93677)
                     /locus_tag="LK694_000295"
     CDS             complement(93492..93677)
                     /locus_tag="LK694_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051196.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            93892..94263
                     /locus_tag="LK694_000296"
     CDS             93892..94263
                     /locus_tag="LK694_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK5051197.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(94413..94485)
                     /locus_tag="LK694_000297"
     tRNA            complement(94413..94485)
                     /locus_tag="LK694_000297"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(94450..94452),aa:Gln,seq:ctg)
     gene            complement(94531..94959)
                     /locus_tag="LK694_000298"
     CDS             complement(94531..94959)
                     /locus_tag="LK694_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051198.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(95019..95777)
                     /locus_tag="LK694_000299"
     CDS             complement(95019..95777)
                     /locus_tag="LK694_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBK5051199.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            96072..96269
                     /locus_tag="LK694_000300"
     CDS             96072..96269
                     /locus_tag="LK694_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051200.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            96375..>96856
                     /locus_tag="LK694_000301"
     CDS             96375..>96856
                     /locus_tag="LK694_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051201.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
BASE COUNT        31536 a        16581 c        20641 g        28098 t
ORIGIN      
        1 tttttagaca tgaatatctt atttttattg ctatttttct taattttcct tctaacatat
       61 aggaaatgtc aaaactatta tgtgcgtata ttgactgcat taccgttttt cttaaacctg
      121 attatttatt ttggtaatac aatgggtcaa agctttactt acgtcaatgg aaataaaagg
      181 gcaatgattt ggagtagtag taacctgaat aatctcttta cagagttagg aacgaaactg
      241 tcactttttt atccaggtac ttggatagct actttagtag tcttggcatt attactatgt
      301 ttaattgttg gaatttattt aagttttgac aataagaaaa cttctatttt tttagttatt
      361 ttgatgataa tgggattttg ttcaaggtta attatgggat tttctcccac agtatgggct
      421 tccggaatgc ggacgtacta tatactttat gttgttatcg ccatcttagt actaatggca
      481 gttaaagagc taatgaaaag catgagtgta caaaaaaatg aatttatgca atttggatta
      541 accgtgcttg gtatttgtac ctttataata acggtgataa acagataata tacaaaaaat
      601 ccctcgcttt agtattaggt gagggatttt actcgttttt cgattaattg ttttaacact
      661 tgcaagtttc tacgagtagc ttgtttccga ccttcatata ctcacctcag gctgtttaca
      721 tgattgtacg ttctttttgt aaaggataca gtgatatgta tataatggca taaagaaacg
      781 ttcaaacaaa aaaactcgtg aagcaaaatt aacttcacga gttgtagtgc agatgatggg
      841 actcgaaccc acacgagcta acgctcacat gaccctcaat catgcttgtc tgccaattcc
      901 aacacatctg cgtaataata acacactaat gatagcgagg ttttacatag ttgtcaagct
      961 caaaaatggg aggacttatt aagatggaga aatatgacgg agaattttca ggactgggaa
     1021 tgatcttagg tgtattgatt ggacttgcat tcggacgttt tttgcttggt tttatgctag
     1081 gaatcatttg tggaattgca atggattggg cagcgaatct atggaatgac tatcatgaca
     1141 attaagttct caacgcttgt ttgtgttttt ttaatactta cgtttttcct aaataagtgg
     1201 caaaattttc taggatggag tatgatattg aaatagtaaa taacaaaaaa tttggaaagt
     1261 tctaactttt tgaaatgagg gattggctaa gtgatgagag aatttgaaaa atccaacaaa
     1321 ttagaaggtg tcagttacga tgttcgcggt cccgttctag aagaagcgga gcggatgcaa
     1381 gaagagggca ttagtatttt aaaattgaac acgggaaatc ccgcaccttt tggatttgaa
     1441 gcaccaaacg aagtcattcg tgatttgatc atgaatgccc gtgactcaga aggctactca
     1501 gattccaagg ggatcttttc tgctagaaaa gcaattgagc aatattgcca actgaaacat
     1561 tttccgaatg tgacgatcaa tgatatctac accggcaacg gagtgagcga attgatcacg
     1621 atgtgtatgc aggggttgct ggacaatgga gatgaagtac tggttccaat gccggactat
     1681 ccgttgtgga cggcttctat tgcattagct ggaggcacac ctgttcatta tatttgcgat
     1741 gaagaggcag aatggtatcc tgatattgat gatatcaaat caaaaataac gagtcggaca
     1801 aaagctattg ttatcattaa tcctaataat cctactgggg cactttatcc aaaagaatta
     1861 ttagaagaaa tcgttgaagt agctcgacaa aataacctga ttatttattc cgacgaaatc
     1921 tatgatcgct tagtgatgga tggtctagaa catattccga ttgctacatt agcgcctgat
     1981 ttgttcgttg ttacgctgaa cggtctatcc aaatctcatc gtgtagcagg atttcgagta
     2041 ggctggatgg tcctaagtgg agataagtca cacgtcaaag gctatataga agggctgaat
     2101 atgttgtcct ccatgcgttt gtgttccaat gtattgtctc agcaaattat ccaaaccgcg
     2161 ctaggcggtt atcaaagtgt cgatgagctg ctcctgccag gcggaaggat ctatgaacaa
     2221 agagaatata tctataatgc aatcaatgat attcctggtc tttcagccgt aaaaccaaaa
     2281 gctgcttttt atatattccc aaaaattgat acaaagcgat tcaatatttt ggatgatgaa
     2341 aaattcgttt tggacttttt acatgaacat catattctac ttgtacatgg tggtggattt
     2401 aattggaacc agccagatca ttttcgaatc gtttatcttc ctaaaatgga tgatttgaaa
     2461 caaacagcta agaaaatgag agaattccta gcaacctatc gccaaaaatg attttaaaaa
     2521 ccaaattttt cagtaggagg gccacagaca aacacactga ggctctcttt ctagtggagt
     2581 tttcttaaat aactaaagta tattatttag ttgcaagtct tttcttgaag tttgcgactt
     2641 cctaaagtaa aatacgaata gaatgcgaat acgcatattt tggggaggat acttatggaa
     2701 acgttaatcg agtcctttgg aacgatcatt aaagagattc gaaaagaaca aaaaatgacg
     2761 caaaaaatgc tttcgcatga tatttgttca caaagtgtat taagtcggat cgaaaataat
     2821 gaggaactgc cgaatgtatt ggtgatgatg cagatttgtc aacggctagg ggtaacgatt
     2881 gatcatgtga tgaatctctc tcgaaaaact actcggacaa acgataaatt atttgaactg
     2941 ttggatagtt gtttccaaaa acgtgattat cggaagttag aacaagtact aaaatcttct
     3001 gatatttctg aaaatctgta tcaagcaact gactttcaac gctattatta ttatctcggt
     3061 gtttgcgaat tcacgttgcg tcaaaatatc tctcagtccc tttattattt gaaagaagca
     3121 ttaagctact ctagccaaaa agaaagaatc catgtttctg acacagaaat acaactgatt
     3181 agttgtatcg gaaaaatata tggtgtggca ggaaaaacgg ctgaagcacg ctactatttg
     3241 agtcgaagca tccaactgct gcatcaatca gctgatgaac gatcaaaaag tgaattttcg
     3301 caaatttttt ataattatgg aaatttctta ttccatcaaa atgaaatcga cgaagcgtta
     3361 gaacaggtga atcaaggaat ctattgggct caagagaaga atagctacta ttacctcaat
     3421 gatttatttg tgcttaaaag cttaatatac aagcgtaaag aagaaccaga aagagcagca
     3481 ttctatgaag gcttagcaca agctgtgaaa caaatttcaa aaaatttgta aatggtgtag
     3541 accattcttg acttcttctc taaataagag tactttagag gtagaaaaac aaagggagag
     3601 aatagaatga gaacttttat ctatgctgac aaattctttt tagcgtcagg tgtcaaacat
     3661 gcaggctatc ttgaaattac cgatggactt tttggtgaat acaccaaaga actacctgag
     3721 ggagaagtca acatcattga ccaaagcggc aaatggattg ctcctggttt ggtagataca
     3781 catattcacg gctatatgaa tcatgatgta atggataatg atgcagaagg catcaaagtg
     3841 atgtctgaag gtctgttatc ttgtggagtg acttcatttt taccaacaac attgacgtca
     3901 agcaaagaac gtttaaaaga tgtagctgag acaatcggca aaatgtatca ggaagcacca
     3961 ggagctaaaa tcaaaggaat ctattttgaa ggtcctttct tcacagaaga acacaaaggg
     4021 gcacaaaacc caagttattt cggggatcct gatttggata ctttccatga atggcaagaa
     4081 gcttcaggtg gtttaatcaa aaaaattgct ttagcaccgg aacgtcaagg tgtagaagag
     4141 tttgtcaaac aagtgactga agaaggagta gttgtttctc taggacacag taatgctaca
     4201 ctagaagaag cgcaaaaagc tgttgaagca ggcgcaagtg tatttgtcca tgctttcaat
     4261 ggtatgcgtg gattgaatca tcgcgaacca ggtatggtgg gtgcgttatt atctttagaa
     4321 catgtctttt ctgaattgat ttgtgatggc catcatgtac atccaaatgc tgcgggaatc
     4381 ctaatggaaa aagcaggaca tgatcatgtc gcattaatta cagactgtat gatggccggt
     4441 gggatgcctg atggaaatta caatttaggc gaatttccag tagtagtaaa agaaggaaca
     4501 gcacgtctag aatctggcaa cttagctgga agtatattga aattgaaaga agcaattaaa
     4561 aatgttgtag actggggatt agcaacacca gaacaagcag tcatgatggc tagtctgatt
     4621 ccagcaatca gctgtaaaat cgatgataca tgtggaaaaa tcgctcaagg acgcgatgca
     4681 gactttatcg tattgaaccc tgatatgaca ttggaagcaa cttatctaga tggcgtggaa
     4741 cgtttccacg cataatataa tttaagttaa ccttaaaact aaacttctta agtttggttt
     4801 taaggttttt tttgtgttct agtaaaaatc actttacata agtttcaaaa gtttctttaa
     4861 tttcctattg ttcaataagt aacaatgtaa agataaaatt aaaaatgtta atacgtatta
     4921 gcaaaataaa agcttttttt attgtgaata aatgaacata attaggagga gaaagatgaa
     4981 cttatttgac atttttcgat cgactttgac agatatgtcg attgtcagtg caatcacttc
     5041 aacagttttt attatattat tagggtttgt ttgtagaaaa aaggggattt tttctgaatc
     5101 tgttggaaaa attctatcga aagtcgtctt aacagtggct ttgccagcac tggcatttcg
     5161 agctttcatg caagatatca atacagaatt actgatgcaa ggaatcaatg tcttgatttg
     5221 gggattagtt atttatatta ttcttattct agtttcaaaa ccttttttct taagctataa
     5281 aggggataag caaacaacgt tgcgtgtcct gacgatcttt ggttctacga cctttttcgg
     5341 aacgcctatc gtcagtgcga tttatggtcc tgtcggcgtg atgtattcct caattttcaa
     5401 tattggctac cgaatctttt tgtattctta tggatacata aaaatgagtg gcttgaagat
     5461 ggaagctaaa aatatcaaag cgatgttcgt caatccaatc attatcgcta cttttgccgg
     5521 ccttttcatt tggctgttcc aagggtatct gccacaagtg tcagttacag gtgcagaagg
     5581 agagataagc caagtagcgt ttttgcgaat tgaccagact gcaccgtggc tatttcagcc
     5641 gatggattat ttagctggtt taagttcacc attggcgtgg ctagcaatcg gcgcaacatt
     5701 aggtgaagta agcttcaaaa aagctgcatc tgaaaaaact tcatggtatt acagtctagt
     5761 caaagtggta tttgtacctg cattgaatat cgtgattcta gcagttttga cggcaacgaa
     5821 tattctacca gtcaattttg aagcactggc aacaatcgtt atcatgatgg ctacaccagc
     5881 tgcaacagtg gcagcagcct atgctatcag ttttgatagg gaggcacttt tagcctccaa
     5941 tgcatcctta ctgtcaacta tttttgctgt ggtactgaca cctgtatgga tcgttgtatt
     6001 acaaatgatt catggcatag gtttattttc ataagaaatg gaggaagaaa gtatgagaat
     6061 cgcatgttac ggtgttcgtc cgaacgagat agattttttt gaaaaattga atatttacca
     6121 ttatgatctc agtctttatg aagaactgtt gacacatgac aatatcgtgt cagcaaagga
     6181 ccatgatgct gtattgttga ggggaaattg tatagcagat gagaccaatc ttgcaaaaat
     6241 gcaggaatat gggattcgtt atgtttttac acggactgtg ggagtcaatc atatcgatct
     6301 acaagcggca gctgattttg gtatgacagt agcccgtgta ccatcgtatt cgccaaatgc
     6361 gatagcagag ttatctttga cttttgccat gatgcttttg agaaatactg cgtatacgac
     6421 gatccgaaca tctttcaaag attttcgagt ggatgagcaa atgtttagtc gtgaaatcag
     6481 aaattgcaca gtagggatca taggaacagg caggataggg ttgacggaag ctgttttgtt
     6541 taagggacta ggagccaaag tgatcggcta cgatattcat cagacagaag ctgctaaaaa
     6601 agtattgacg ttccactcgc ttgaagagtt attagccaaa agcgatattg tcagtattca
     6661 tgtacctcat atcccaggtg aaaacgatca aatgatcaat gaaggatttt tagctcaaat
     6721 gaaaaaaggt agcatcttga ttaatacagc ccgtggagag cttcaagata atgaagctat
     6781 tttaagagct ttgaaaagca atcatttagc tggttttgca accgatgttt ttgcaaatga
     6841 aacagaggtt tttttccgat ccttcaagcc atgggagacg attccagacc ctgctatcca
     6901 gcaattagta gaaatgtacc ctagagttct tgtaaccccg catgtaggtt caaatacaga
     6961 tgaggcatta tcaaatatga tttcgacaag tttcgagaac tttcgtgaaa taatagaaac
     7021 tggaaaaacg aaaaatgagg tatctcttcc taaagcccgt caattaagat aagaaaacat
     7081 ggatattcgt caataaaatg tttaaaaata gtccaagtaa acagttgtta cttctgtata
     7141 cttggactat tttgtatggg ataattcgat gactcaacag taatctgtga gctttttccg
     7201 cccattcttt gtcaattcat aacaggttag cggccggccg atttccaaat atttgattct
     7261 ttcgttcaat atatcttgtt ctactaaaaa attcaaatat ttcttgacgg atatacgtga
     7321 cagtgagctt ttttgagcca gttcttttgt tgaaaaaata gtatcttctt ttagaataaa
     7381 tgcacttatt ttttttaaag tcagttttga cagaccttta ggcaaatcga tgaatgatgg
     7441 tatttcgtga atgggagtgg tgtggttgaa tacttgatcg atcaatttct gatcggcttt
     7501 ttcagtcgtt tggattgtct gagagaaagc aagatacttt tcgatcgcca gctgaaaacg
     7561 ttgcaaagtg aacggtttga tcaaataatc taaggcaccg tagcggatca cattctttat
     7621 ggtttcgata tcatctgcag cagtgatcaa aatcactggg atatcatttc cttgctgtct
     7681 aagccaagat aaaaaatgga taccactttc gccaggcatg tatacatcaa gcagcagcag
     7741 atctgcgttt ttttgtctga aatatttttt tccttcttct acatttctga aactttcgat
     7801 tacgttcata ttagggatgc tttccacaaa agtacgattc aattctgcaa ccattggatc
     7861 atcttcgatt atcaggacat ttgtcatcat tttgatcttc cttttttggt aattctagat
     7921 agacgattgt accgccattt gtttgtttgg aaaattctaa aaatccaccg tggcgtttga
     7981 cgattgaatg gatgagattc aatccaaacc cacgattgat tcccttcgtc gaaaaaccta
     8041 gcgtaaatat tttttctgag atttcttctg atatcccgac accgtcatcg cagacggtca
     8101 cgatgaatac ttcagcttct gtgtcatagt ttaaatgaat ccggacctgt ccgtttcttt
     8161 catgtttttg tagcacggct tcttttgcat tatcaagtaa gttccctaac acttgcaaaa
     8221 gtgaatggat ggagtcatcc atctccattt tagggataaa agaatcgctt tctaacagca
     8281 tttcaacaga ttgttctttg gcttcgttta ttttgcctaa taaaaaaccg gcaattgcag
     8341 gtactttgat cagacttgag acttggccaa tctctttttt ataattttga tttagctgct
     8401 ggataaaatg ggtgacttct tcatacttct tcaaatcaat caaaccagaa atgacatgaa
     8461 gtttattcat gaattcatgg gtttgagctc tcatggagtt tatatattgt ttcgtccccc
     8521 caagctcctc cattaatttc attacttctg atcgatttcg taaagttgcg actgctccta
     8581 aaaaacggtc attgctaaaa agaggtgtaa cgctagaaat agtttctatc ccatttaaat
     8641 acatggattg agtccgaacg gtcgtttgat gctcaatcgt ctctttaaac aaaacgtcat
     8701 atagattatc actgattttt ttcctacttc gaaatccaat agctgctctg tttggtagaa
     8761 aatgtttttt gcaggtgtat tgatcaagag gatagtttta tcagaagaga tggctaagat
     8821 tccttcatct acttcatttt cgataatcgt tttttcactt aactgttctg ctatttcttg
     8881 tggttcaagt ccaagcagta cttttttcat tttttgggca agaaaaacgg cgcctatgat
     8941 gcctacgcta agtccaataa tcaatgaaaa aacaatcatt ttttgtccat ctttgacttg
     9001 attgtcaatc gtgttgtttg ttaagccaac acaaactgca ccaataatct cttgatcata
     9061 ataaataggg acgaaatagc ggaagccgaa ccctaacatt ccttcttttt cagagaaaaa
     9121 ttcttgtcct ccaatcactt ttttcacatc ttcatgactt gagaaaggaa gaccgatgta
     9181 acttgggtct gcgtgagaat atcgaatgcc ttcagggtcc atgacaacga taaaatctaa
     9241 attcgatgca atcatgattt catttgttat tccttgaatc gcatgtgtcg ggtcaccagt
     9301 ctgcagcttt ttttggatga gtttatcatt ggcaacgatt ttagcaatgc tagagatttt
     9361 atctgacaca ctagatcggt tcatcgaaag aataaaattc ctgatcaata tggcagaaac
     9421 aatcaaagaa acgaatgtgg agaaacaaac gacaaggatg atcgtgttct ggagctttaa
     9481 tggttttttc ttaaaaaaag gaaacatagg ctacctcgaa agaatttatt gtgaaaattt
     9541 taacatgaaa acaaaaattt aacaaattgt caacgataaa cacaaccttc ctataaaaaa
     9601 caccactgag tcggtaaaag aagctataca gaattttcaa acattggtat gatagacgta
     9661 gaaatggaag gaggcgtgta tatgataggg ggaatcgcac ttgagacgat ttattggtat
     9721 acattgctga tttgtgcagg attggcagtc ctattgatta tttttggaga tgtattcgat
     9781 tttgatggcc cgcttgatcc gatgcttatt atcccttggt taactttcac aagcttattt
     9841 ggttttctag gtgagcgttt aacagatcaa tcatctttat ttttatttat tgctagtgga
     9901 ggcatatcga tagtgttggt atttttgctt aatttctata tattgatgcc gatgaaacat
     9961 gccgaatcaa cgatgtcgtc ttctgaaaaa actctggaag gacaagtagc tgttgtgatc
    10021 acaccgatcc ctgtacgagg tatgggagaa attcagttga aaagtgttac cggttccatt
    10081 agtcgtccag cttgttttta cagcccccaa gaacagacag ctgagcgggg aacgaaagta
    10141 ttgatcattg agatcaagga aagaatctgt tatgtcatgc cttatgaaag catgataaaa
    10201 gcttaaagga gcgtgtgtta aatggaaata ccagttgttt ttatcgcgat cgcaatcgca
    10261 gctttgattt tactgatgtt gctgatcgtc tttgtagcga agtaccagac agcaaaaccg
    10321 gatgaagcgt tgatcatcag tggaagttat ttaggaagca aaaatgtgca cattgatgaa
    10381 agcaataaca aaatcaaaat tgtgcgtgga ggtggcgcat tcgtcttgcc agtttttcaa
    10441 cgttcgaatc ggatcagtct attatcgagt aaattggacg tctccacccc tgaagtgtat
    10501 acggaacaag gcgtaccggt gatgtgtgat ggtacttcga tcatcaaaat cggttcatct
    10561 gtagaagaga ttgctacagc agcagaacag tttttaggca agacaagaga agaactggaa
    10621 aatgaagcaa gagaagtatt agaaggacac ttgcgctcga ttcttggttc tatgactgta
    10681 gaagaaatct atcaaaatcg cgataagttc agtcaaagcg tacaagaagt cgcttctgta
    10741 gaccttgcaa aaatggggtt gatcatcgtt tctttcacta tcaaagaggt cagagacaaa
    10801 aacggttatc ttgactcttt agggaaacca agaatcgccc aagtcaaaag agatgcagat
    10861 atagcagaag cggaagctct aaaagaaacg cgtatcaaaa aagctcaagc agaaaaagaa
    10921 tcacaagcag ctgaattaca gcgacaaaca gagatcgcag aatcattaaa agaaaaagaa
    10981 ctgaaattag caacctacaa acaagaacaa gatgttgcta aagcaaaagc agaccaagcc
    11041 tacaatttag aaagtgcccg agcacaacaa caagtaatcg aacaggaaat gcagattaaa
    11101 atcgtagaac gtcaaaaaca aatcgaactg gaagaaaaag aaatcacacg tcgtgaaaag
    11161 caatatgatt cggaagtgaa gaaaaaagca gatgccgatc ggtacgctaa agaacaagaa
    11221 gcacaagctc aaaaagtcaa agaagtgact gaagccgaag cagaacgatt ccgtgtggaa
    11281 gcactggcag aagcggaagc caataagaca cgtttagctg gacaagcaga agccgaagca
    11341 gcactagcaa aaggaaaagc agaagccgaa gcaaaacaaa agatcgccaa tgccttcaag
    11401 gaatatggcg aagcagctgt cttgagtatg gttatcgata tgctaccgca attgatgcgc
    11461 gaagcagcac agccgctagg aaatatcgaa aaaatctctg ttgtcgatac aggaagcagt
    11521 tcaggagaaa ccagtggagc gaaccgagtg acaaattatg ccacaaattt gctttcttct
    11581 actcaagaaa cactaaaagc gacaacagga cttgacttga aggaaatcgt agagaatttg
    11641 tcgacacgtg ggactactag ccaagtaaat atgtatgaag cgccaaaaga ggcagaacaa
    11701 aactcgatga aggaagaata aagtagtaca cttcaccgtt caatcaaaaa tggaggaaga
    11761 ctttttaaag ttcttctctc cattttttta ttttattgca aaagaaaatt agtcctcgct
    11821 atttttcttt tgtttttgga tcttagcttc tatctcttgt aaatttgttc ttagcaatcg
    11881 ttctttcttt tggagttcga ccaaatcttc tgatagttct tccttctttt ctgtttcagg
    11941 aggacgaaga aagaaattga taccgattgc aatgaatgtc cctataaaag tatctagtac
    12001 acgttggata gcatacaaag cggattcgcc ttgagggatg ctcaacgcaa tcagcaacaa
    12061 cgtagctatt gcggaaatga taccggaatt attgttaata ccatctgaca cgacgatcac
    12121 aataataact agaataggaa gcaagatcaa ttctaccaaa aaatcattgt gaaaatagct
    12181 tttgacaaaa aaataaatga tagctaatgt accacctaat gtattgccga tgatcctaga
    12241 ctttccaaaa gaaacggttg tagtcaagtc ctgacgcaaa gaaaaaacag cagataatgc
    12301 agcaatcatc gggattcctc gttgagtcaa atgaaaaaac aagatacata aaaaaacagc
    12361 taaggcactt ttgatcgtac gcatacctag tcgaaaacgg ccaaattgca tagagattct
    12421 cctttttggt ctttttacta tcatacctaa acttttgtct gatagctagt ttttcattat
    12481 tcaagtataa ttaaaataaa aaagggggaa gtaaatgcgt ataggaatgc gaacagtcaa
    12541 aacagtgatc agtgtcttct tttcgatggt cacagcgggt agcttatcgc tgttgtattg
    12601 gccagctgca gggatcattg cattattaag tgtaggaaat accaagcgct ctacattgcg
    12661 tacgggcatg aatcgaatcg cagcctttat attagctacg gcaacagcac tgttcagttt
    12721 ttttgctgtt gggtatacta tttttggctt cagtcttttt ttattcctgt tcatcccgct
    12781 tgcctctcgc ttcaaattaa ccgaagggat agtagtaaat tcggttttgg tcactcatta
    12841 tttagtggag gaaaacatgt cttggcagct gatcggcaat gaagcgggat taatggcgat
    12901 tggattagta tttgctttgc ttgcaaatgt ttacatgcca gatcgtacga agcagctgaa
    12961 ggaaaaccaa atacagatcg aaaaagaatt tcgaaatatt ttgagacaaa tggcagaatt
    13021 tcttttatca gaaaataaag aagatgtgca gatcaaatgc gaacatttac tgacattcat
    13081 tcgcgaaagc caagaagatg ccagagaaca tcaagaaaac tactggctca gacagccgct
    13141 atactacgaa acttacttct ctatgagaag agcacaggcc aatgtgatta aagacatgct
    13201 ggaaaatcta gagagaatcc agcaaccagc ttattatgga aaacatatct atggtttatt
    13261 gatttatact gcagaaacat tttcagagtc aaatgatggc agacaaattt taacacgtat
    13321 tgaagaagtt tatgtattgt atcggcaaat gcctctgccg actagtcgcc ctgaattcga
    13381 ggatcgagca gaattgttcc agtttttgca gtcttttaaa agttttattg aaatcaaggc
    13441 agaattctcc caacaaaaaa tccaaaaata gaggttgtga attcagctaa tattcaggtc
    13501 aaaaagctga tttcacaacc tcttctttaa acggtgttct cctgtcaact cctcggtcac
    13561 aagtcacaat attcaacaaa catgagaagc tgttttttga aattgctcct tgtgactcgg
    13621 agctgaacgg taggctcaca acctctttct taaacggtgt tcaaaagctt tcctctgcgg
    13681 taataagtcc agccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac
    13741 ttgatgcaga acagcttttt cacaaccttt taaaattaca tatttttcca tagaacttgc
    13801 atttgatcca agatttcgat gacatctttt gtcaagaaga gagagcgatt gggcaagtca
    13861 ccttcaatca tgtgtaccat attctttatt tcgtaattca ttgcttcaga tgtattcccg
    13921 ctttcgatga attcctttgt accatctgta aataagatct ccgcacgatc agcacgcggg
    13981 taatcagcaa tcgtcacata accgttctca aaggcaacga taccgacttt tggcattttt
    14041 gcctgaaaag tcaagctgac agcagcaagt tcattttctt tgttacgcaa gatagtgacg
    14101 gattgttcgt ccacgcctgt ttcaaaagga accattgtag aggcaactac ttcaggttga
    14161 gagctcaaaa agaaacgagc gaaagataca gcatacgtac cgatatccaa cagagctcct
    14221 ccagctaatt ctggattgaa gaaccggttt ttagggtctg gttctttgta actgccgaaa
    14281 ggtgcttgga tcatttttaa agcccccagt tttcctgtat ccattattga gcgtaactgc
    14341 tgataaagtg gcatgttgaa gattgtcatc gcttctgcta agatcaaatt cttttcttct
    14401 gctaagcgca tagcgtcagc cagttctttt ttattcatcg tgatggcttt ttcacataga
    14461 acgtgtttgc ctgcttccaa tgctgccaag atatggtcga tatgctgtct atttggtaca
    14521 gcaatgtaca cgatgtcgat ttcagcatcg tctaacattt cttgatagct tccgtaagct
    14581 tttggaatat tgtaacggtg agcgaagttt tccgcttttt ctgaagttct ggaagcaact
    14641 gctgttagtt cgctcgtctc ttgattgaag ctttcggcaa actcatgtgc gatggtacca
    14701 agaccaacga ttccccattg atattttttc atctgaaaac gctccttcat agttggattg
    14761 agtttcttgt ctgtctatta ttataacaaa aatcacgaag aataaagagg agaggacttg
    14821 aaacattaaa aaaaatgaaa gcagcgattt aagaggtgct ttttgatcaa aacttaagta
    14881 gaatagttaa aaggagggga taaacgtgca tcaagtatat attgatctta ttatgagtgt
    14941 attattagaa cagtttgaaa ctgaaaaaaa ttttttagat gaatacttgc tgatcagtcc
    15001 agaacaatgg caaaaatgga aagatggcga gaaaaatctg ccatctgaag cgatgcagaa
    15061 agtaaaaaat cttttcactg attatgaatg gatgctaacg caaaagattt tgcgtcaaac
    15121 gattattttt cccgaaaaac gaaacgttgc cgttgcagag tacaagcaaa tcaaaacacg
    15181 catcgcgcaa aaatggctta gcagcaaagc agcaaaagtc gagctgatcc ctttgaaaaa
    15241 taatcagcag gctggtagat accttgactt gaaagtaagt attcaatatg atgaatgggg
    15301 ctatgatgat attttgaatt ttcgtctgcc agcagatgtt caaaaacaga tcgaaggaga
    15361 aaaaatcgaa cttctcgatt gggtgaatga aaatctggaa gaaacttacg ttcatgaggc
    15421 gaatggggaa cgtgaagaac aacgaaagga tttttcaagt gaataaattt ttaggatttg
    15481 gagcagttgt gattatgatc gcagcaatgg gctatgttat ttttagcgat gatgattcgc
    15541 atgttcaagc cggcaatcaa gcagtcgaac gaacaagcac caaaacaact gctagtaaag
    15601 aaaagaacag tcaaacaacc agcagcaagg taaaggacaa agaaacaaaa gatcgtacgt
    15661 ccaaacaatt gccagatgtt aaaacagacg attggaagct agttttggtt ggtccagatc
    15721 acaaattaga aaaagaaatt gatgagtcta ctcaattggc atcattaagt aacggatact
    15781 tgatcgacaa acgcattgct ccagaatatg aagcatttgc caaggctgct gaagcagctg
    15841 gatttccatt agtgatggtc tcagcttacc ggtctgtatc atcccaacaa caagttttcg
    15901 aacaaaatgt ccaagatgtg atgagtcgtc aaggtgtatc agaagaggag gcgacaaaga
    15961 ttactaaaga aacgattacg gagccaggat acagcgaaca ccacacaggt ctggcagtcg
    16021 atgtcgtcga tcaaaactgg tacaacagtt atccttctac tgtattggat gcaagttacg
    16081 gagatcagcc tggagctaaa tggatcgcag ataatgctgc gcagtatgga tttatcgttc
    16141 gttatccaaa aggacacgaa gatataacta agatcacata tgaaccttgg cacttacgtt
    16201 atgtcggtaa agaaaacgct gagtatatca ccaaacacca attgacgcta gaagaatatc
    16261 tgaatgaatt aaatgaaaag taggaattga tcatggcgaa aaaaaagtgg aaaaagcaaa
    16321 agcgcaagaa tatccgttgg ccagcagtgg ttgccggtgt agcagtgata ctcttagcct
    16381 ttgttttttc cttgaagaat ctgtcttctc cttttactga taatcagcaa gaaaatgaag
    16441 cgcaatccca tcagcaattt attgaacggc ttgttccgca tgcccaagaa ctacaagatg
    16501 gttatggagt gcttcctagt atcattttag gacaagctat attagaatca aattggggaa
    16561 aaagtacctt ggctagcaaa tacaataatc ttttcgggat caaggcttat ggggatcaga
    16621 agaaagtaag cctggaaaca aaggaatttg ttaacgaaga atggataact atccaaggag
    16681 acttcaaagt gtatgattca tgggaacagt ccatggatga ccatacacaa ctgttcgtcc
    16741 aaggagtaga ttggaatcct gcgctgtatg aaaaagtgat tacggccacc aactatcagg
    16801 aagccgcaca agcattgcaa gatgcaggtt atgcgacaga ccccggttat gcgcaaaaaa
    16861 tcatccaggt catcgagacg tatcaattga atcagtatga ccggagatag caaaggagaa
    16921 gtggctgatg gcagaacaat ttacgccaga aatcatgacg gaattgagaa aagatatcgt
    16981 aagagtaccg gaaatcattt tagaagcaag cgggatcaag atatttggaa aattgatccg
    17041 ttcgatcatc tttactacgg atattgcgat catacgtaat acaaatgcga atgcagtaat
    17101 tgctgtgtat ccttttacac ctcatccagc gattactaaa agcattattg aggcagctga
    17161 cattccagtt ttctccggtg taggtggtgg tttgacacaa ggtgctcgtt cttcctacat
    17221 gagcatgttc gcagaagcgc aaggttcgtt aggggtcgtt ttgaacggtc ctacgccttt
    17281 agagacagta aaagaagttt gtcgagtagt tgatattcca gttatcagta cggtaacgtc
    17341 gaaatataca gaaatagatg aaaagttagc agctggtatt tcgattatca atatcagtgc
    17401 aggtaaagaa actgcagaag cagtacgcca ttttcgtaaa aaatatcctg aacttcctat
    17461 cattgcgaca ggtggtccaa cggaagaaag tattgctgag accattcaag caggagcaaa
    17521 tgccatcaca tatacgccac caagtagcgg agagcttttt agtcgtaaaa tggaaaaata
    17581 tcgggaaact gagaaaaaga atcatgaata acaggtgaga atcaaaagac gaggaagagc
    17641 agatagtggc tcttcctcgt cttttgtact ccagtactat ttgttcagaa ttcaaaaaaa
    17701 agagaaattt ttttgttttt ttacctgtaa tgtacataag aagagttaaa accttttgtt
    17761 attattagca ttctaacaaa aaaatgtctt gatttattga agcgctttct atagaatgaa
    17821 aaacgaataa aaactaaaaa aggcacccga caatttagta gcagctaaat tgtcggggct
    17881 tgaaaagtca gcgggaactg accaatcaag ttgccatagt caatgcttgt gcatcgacgt
    17941 gtatattgta cctaatttca tacagaatta ctagttattt ttctgttttt tagggaagag
    18001 cgcacacgaa gaagcatgct tatttagcta tgtcaaggat attgggcagt tgtctgatat
    18061 ccttttttct ttgcgtaatt atcctcccat cattgaagca agaagtgttt ttagcgatga
    18121 aagaagaatc agcactatct aatttttctt gaaagtattg agttgttcat cctaggcaga
    18181 acgataaggc aagtcttggt ttttattccc cttgaaaaag atcgtttaat aaatatgttc
    18241 tgtataaaaa tacagtatat tgaaaaataa caatggaagt agaggtgcag agaatgaaga
    18301 aaagaatgag aatcatctta gctttaagtg ttatgttatt cttcttgacg gcttgtgggc
    18361 aagaagatga caaaaaaatc acgctgaaca tggcttggtg gggttcgcaa gtccgacacg
    18421 atgcaacagt aaaagtaatc gagctttatg aagaaaaaaa tccccatgtg acgatcgatt
    18481 atgaatttta tgattatgaa ggatattaca caaaattagg tacactagca gcatcagatg
    18541 atatctggga cattttccaa ctgaacaatc aatttcctca atacatcaat cagattgaac
    18601 cattagatga atatgtggag caagggataa ttgatatttc taatgcagat gaatcctatt
    18661 tagcaacgac gacttacgac gggcaattag ttggactgtc taacggggtg aattcgtttg
    18721 cgattgctta caataaaaat atatttgaac aattaaatat ccctgaacca gcttcaaatt
    18781 ggacatggga tgaatttgaa caaatcagct atcaaatcac tgaagaatta ggaatctatg
    18841 ctatttcaag attcgaagat tttatagctg gctgtattat tcaaatacca caagttgaga
    18901 aaggattgaa tttttataat cgggaagatc tatctaacag cttgggattt gaaaatccag
    18961 actatctaac agattttttc cagctgcgta aaaatctggt agacaaaggg gcccatccag
    19021 aaccaggtgg tggagctgca agtacgacgg atctgtcttt tcaggcggtt agggatagag
    19081 aagcggcaat ggtgtttcta tcaagtaacc agttgacaga aatattgtca ggggctcctg
    19141 aagatatgga gattcagttg attaatccac ctcgcagaaa agcagatgga gaatctggta
    19201 tccctttacg ttcttcgcaa atgctgagta tggcaaaaag ctcagcccat aaagaagaag
    19261 ctgctaagtt tattgatttc tttcaaaaca gtgaagaagc caatgaaatt ttaaaaggag
    19321 aacgtggtgt accaatcatg tcgaatattc gagaacagct cgctcgtgca gatgatgcaa
    19381 ctttgcaagc gacatttgat tatttagaaa tgatttccga tatggacaac ggcgaagtga
    19441 atgtctttga tagtgctttg aatccggaaa ttcaagacca atacaatctt ttagttgaaa
    19501 aagtcattta tgaagaagta acagcagaag aagcagcaga gagtctttat gagtttgcgt
    19561 caaacattct aggatagcag caggaggaag gcatgaaaaa agaaaaacta aaacggttct
    19621 tggatcaaga caatgtggtc ggttacgtat ttgcttctcc attcatccta ggatttctat
    19681 tgataacagt gataccgatg atcttgtcac tttattattc gttcaccgat tatcgtttgg
    19741 gaaatccagt ctcatggatc ggcttggata attatataaa gatgctgagt gatgcccgtt
    19801 ttttgaactc catccgagtc acttttctct atgtatttgc atctgtacct gtgaaactga
    19861 tctttggtct attgattgct tatcttttaa cgaggaatat caaaggaacg actttctttc
    19921 gttctttgta ttatgtgcct agtttgatcg gtggcagtat ttctatcgct ctagtttggc
    19981 gggagttgtt ttctaaaaac gggttagtga atattttcct ggaacgatta ggcgcacctg
    20041 cgctttcctg gtttagcgat caaaaactgg tcatgatccc tcttgttttg atgtctgctt
    20101 ggcaattcgg ttcggcaatg atcatctttg ctgcaggtat caaagagatc ccaggctcgt
    20161 attatgaagc ggcagagatc gatggagcga acaaatggca gacgttttac aagatcacgt
    20221 tacctcttct ttcgccgatc attttgtata acttggttat gcagacaatc gcggcgttta
    20281 tgacttttac gcaggcattt atcattaccc aaggtggtcc gaatgatgcc acgaactttt
    20341 atgctcttta tgtgtataac caagcttttt cttttaattc tatggggtat gctagtgcta
    20401 tttcgtggat catgctgttg attatgggag ctgtcacctt tctgattttc aaatcatcaa
    20461 aaaaatgggt cgttgtgtga atgagaggag gaagaaatga ataatacagt aacgaaatgg
    20521 tttcaatacg gttttatttt gctattaggt ctagtcatga tttatccggt cttatggatg
    20581 atcgcaggtt cttttaaaac ggatcaagag attatgagtg gttcgctgaa tttgattcca
    20641 gagacttttc aatgggaaaa ttacgcaaaa ggatgggcgg ggtttgctgg aatttcattt
    20701 tttactttct ttaggaattc gtttgtcatc tctgcagttt cgacgatagg gacagttctg
    20761 agctcgactt gtattgctta cgcattatcg agaatcgatt tcaaaggaaa aagattctgg
    20821 ttcgtcgtca tgttagtaac gatgatgatt cctgcacaag tgattttgat tccgcagttt
    20881 attatttaca atcggctgaa tttggttggg acgtatgtgc cgctgatttt accacatttt
    20941 tttggacaag catttttcat ttaccagatc atgcaattca tggtcagcat ccccaaagag
    21001 ttagatgaat cagcgattat tgacggatgc agcaagtata gtgtctttac caaaattatt
    21061 tttcctttgc tgaaaccttc gatcatcacg acgatcatca tccagtttta ttggaaatgg
    21121 gacgacttca tgggaccatt gatttattta aacaagccga gatcttacac ggtatcgatt
    21181 gccatcaaat tatttgcaga cgctggatct agtacgactt acgcgagtat gtttgcgatg
    21241 tctacgttgt cgctgctacc ggtttttctg attttcttat tcttcaataa atatcttgtc
    21301 caggggatca gtacaagcgg tctgaagggc tgaatatgaa aaagccaata gaagagatgt
    21361 ttctttacaa gctcaactac tatgtctgtc ggattttttt aaccagtctt atcagtaatg
    21421 ctcttttcgc tttagttcta ggatgtttgt tcactttagg attagtgctg tctgctcgtg
    21481 ttgtctatct gatttatttt ttgatgatgt atccttctta ttatctatct gtccattact
    21541 tggcagaaaa aaaggtatgt tggaggaaag gaaaaatcaa acggatgtgt ttcatcatta
    21601 cgttgtttca gctgtttgtc attatttttg cagttgattg gtatttatta gtgggaaaga
    21661 ttggagcttg gtatctgatt ccttttctat tgtgtctgtt gctttttgta tcccatagtt
    21721 tttttagtac gctaattgat atctgcaaag aagataaagc agatagtctt gtttatttga
    21781 tcaaaaggaa tatctggctg ttgtcaaaaa acgtcaaggg gtttctgtgg tatttatgcc
    21841 ttttgtatgg actgttctgg tgtatacagc atgatttttt actcactgca ttactgtatc
    21901 cagggctgcc atttgtcaca gtattttgct taaaagaaaa ggagaatgac tttaaatgaa
    21961 gaaaaaatat gtacaagtcg gacttggtgg gagagcgcgt tttttttatg aggcaattgc
    22021 ctctacgtat catcaaactt gtgaactagt cggtttttgt gatgtatctc aaacaaggat
    22081 ggattatgca aatcggatac ttgtgaacaa gtatggacag aaaaaagtaa agacatgtat
    22141 gccatcacaa tttgagcaac tactggatga cgtacggccg gctgaagtca ttattacgtc
    22201 agtagatcga acgcatcatg attatatcat tcgtgcaatg aaaaaaggat atgatgttat
    22261 ttgcgaaaaa cctctgacta tcgatgagga aaaagctcaa gaaataataa cggtccaaaa
    22321 acagacggga agaagcctgc gagtcacttt taattatcgt tatgcaccgt atcacacgaa
    22381 gataaaggaa ctgatagaaa gtgatgtgat tggtgatgtg ttttctatcc acttcgaatg
    22441 gctgttaaat acgcaacacg gggcagatta tttcagaaga tggcatcgaa ataagcacaa
    22501 tagcggcggg cttcttgttc ataaatctac ccatcatttt gatctagtga atttttggtt
    22561 gaacagccgt ccccaaaagg tatttgcatt cggtggatta aacttttatg ggatgcataa
    22621 tgcggagaaa cgcggagtag aaatatttta cagtcgatgc tatggaagcg aactagctaa
    22681 agctgatcca tttgctatag acctgtcagc caatgaggaa ttgcgagaaa tgtatttaga
    22741 agcagaaaaa gacagtggat atattcgtga ccgcagtgta ttttccgatg atatctctat
    22801 tgaagataca ctaggcgtaa cggtcaaata tgaaactggt gcgattttga cgtattcgct
    22861 aaacgcgtat atgccgtggg aaggattcaa tgtttctttc aatggcagca aaggacggat
    22921 cgaaatgaaa gtgatcgaaa agtcttacat caatgcaggt ggagataaaa aggatgaagg
    22981 agcagccgtt cttcagtcca tccatgtcta tccgatgttc ggtgagccat atgaggtaat
    23041 cgtcgaacaa gaagcgggcg gacatggcgg aggagatccg cgtttattag atgatctatt
    23101 tggtgaagaa aaagaagaag atccgtggaa tcgggcagca actcacgtag atggtatttt
    23161 gtcgattttg acaggaatcg cagcaaatca ttctattgct tcaggaaaag cagtagtgat
    23221 tgacgagctt gtcagcttaa aagaataaga atagatttac ggaaattact tcccgtagtt
    23281 tataaaatct tgccttattg ccgataggct gtcttttgaa cactatttat tcggaaaaag
    23341 gggctatgac atgctttttg tcatgccttc tttttatgtg ggctgctttt ttagatagga
    23401 gaaaataaaa ataactattt aaacccgaac aaaaaggaat aatgcattaa aaatcattag
    23461 attttacaaa aaaataagta aaccacgttg tcagtttcag aaagtagtgt tagactgttt
    23521 tcaatatttg aaacaacatt ttttattcaa atatggcaaa ctgccttcac gtgcaaaaca
    23581 gttgcaagaa aatgaattta cactattatt agttttacat acctacttat atatcgtcat
    23641 aatatggatg acagtttcta gccagtaccg taaatgctgg actataagta aaagattggc
    23701 tatttcaccc ttcttttctt atgataaagt ggagggtgtt tttctttaac agaaacatag
    23761 taggtttttg cacaaaagcg gttatttcgg tattaataaa tgataaagga gcaaataaag
    23821 tgaaagaatt agttgaacgt attcaaaaag acggacgtat attaggcgag ggcgtattaa
    23881 aagtcgacag cttcgtcact caccaagtag accctgtatt gatggaacaa atgggaaaac
    23941 ggtttgcaga agtatttaaa gaacaaggaa tcacgaaagt tgtgaccatt gaagcttctg
    24001 ggatcgcacc agctttattt gctgcaagag aattagatgt tccaatgatc ttcgctagaa
    24061 aagcaaaaag tttgacgatg gatgaagaat tattgacagc ttctgtttat tcttttacga
    24121 aacaagtgac aagtacgatt tctatttctc gtaaattttt atcttcagaa gacaaagtat
    24181 tgattatcga cgatttcttg gcaaacggac aagcggcaaa aggattgatc gaattatgcc
    24241 agcaagctgg agcgcaagta gaaggaatcg ggatcgtcat cgaaaagtct ttccaagatg
    24301 gacgtcagtt acttgaagaa atgggattga gagtcgtttc tcttgcgcgt attgcttcct
    24361 tgacgaatgg acaagttgag tttcttgagg aggacgcata atcgtggaaa aagaaacaca
    24421 aaatggaaaa gctgcggttt tagggctgca gcatctgcta gcgatgtatg caggagcagt
    24481 agcggtgcct cttttgatcg gtacaggact aggctttaat caagaacaga tgacttattt
    24541 gatttcaatt gatatcttta tgtgtggatt agcgacatta ttgcaattga cagttaaccg
    24601 atttttcgga attggcttgc ctgtcgtttt aggatgtgcg atccaagcag tagctccact
    24661 gatcttgatc ggaaccaatg aaggagtagg agctatctac ggatctatta tcgcttctgg
    24721 gatctttgtt gtgttagttt ctggtgtctt ttctaaaatt aaaaaactat tccctcctat
    24781 agtaacagga acggttatca ccgttattgg tttgactttg attcctgtag caatcgagaa
    24841 aatgggtgga ggtaatgcaa cagcagctga ttttggtgac aaaacaaatc tgctacttgc
    24901 atttgtgacg attctcctaa ttgtaggcgt gcaaatgctg gcaaaagggt tcattcgttc
    24961 aatcgcggta ttgattggtt tagtcggagg aagcatctta gctgctttca tgggaatgat
    25021 tgatctgagc gcgattggag atgcgccgtt gttccatgtt ccacaaccct tttattttgg
    25081 taaaccaact ttcgacgtat ggtcgatttt actaatgatc atcatttcca tggtcagcat
    25141 ggtagaatca acaggtgtat attttgcact tggtgacatt actgggaaaa atgttggaga
    25201 agaagaattg aaaaagggat atcgtgcaga aggacttgcc gttatcttag gcggtatttt
    25261 caatacattc ccttatactg gcttttctca aaatgtcgga ttggttcaac tttcgggaat
    25321 caaaacaaga aaacccatct atttctctgc attcttcttg attgttttag gattattccc
    25381 taaaatcggt gcgttagcac aaatcatccc tgagccggta cttggtggag gaatgttagt
    25441 catgttcggg atggtggcag tccaaggaat ccgtatgctg ttggaagttg atttcagcaa
    25501 tgataaaaat ctattgattg ttgctgtttc tgttggattt gggctgggtt tcaatgttac
    25561 gccgacatta ttcagtcaaa tgccgcaaac attgcaaatg ttcactggaa atggcatcgt
    25621 aatgagtagc attacggcta tcatattgaa tttgatcttt aatggattga aaaaagatga
    25681 agaaatattg gaagaagcct tcaagaagta atagagcaaa agaaaggttg tgaaatcagc
    25741 gttttgacct gattgttaga gaaacaaacg gaaaaatagt tcctcgtatt ttgccgtttg
    25801 tttgagctaa taccgagggt cagctgattg aacaccgttg aaggaagagg tcgtgagcct
    25861 gccgttcatc tccgagtcac aaggaacaat ttcaaaaaaa agcttctcat gtttgttgaa
    25921 tattgttcct tgtgaccgag gagttggcag gagaacaccg tttatccata aaaaggaatg
    25981 tgacctaact tttgtcacat tccttttttt tatctttaat aaacacgaac aataaaatgt
    26041 ttttctttgt taacattcga aatttctttg acatcaaaaa aagtgattgt taaaatatga
    26101 atgtagaaaa actgggagga aaaacaatga cagcaaaaat ctctgtgatt atggggagta
    26161 cttcagattg ggaaacaatg aaagaagcat gtcaaatatt ggatgaattc ggtgtgtctt
    26221 atgaaaaaaa ggtcgtttct gctcatcgaa ccccagattt gatgtttgaa tatgcacaaa
    26281 acgctcgaaa aaaaggaatc aaaatcatta ttgcaggagc agggggagcc gcgcatctgc
    26341 cgggtatggt cgcatcaaaa acaactttgc cggttatcgg tgtaccggtc caatcacgaa
    26401 cattaaatgg tttggattcg cttctttcga ttgtccaaat gcctggagga gtacctgtag
    26461 ctactacagc aatcggaaaa gcgggagcaa ttaatgctgg actacttgcg atacagatgc
    26521 tttccatgta tgatttagaa ttggaggcta aattggctga aagacgttca atgttggcta
    26581 aaactgttat agaaagtagt gatcaacttg gttaaacctt tgttgcctgg ttcaacgatc
    26641 gggatcgtcg gtggcggtca attaggtcgt atgatggcga ttagtgcaaa agaaatgggc
    26701 tttaaagtag gtgtgctgga tcctgtgagc gattgtccag ccgcacaagt tgcagattgg
    26761 cacattgaaa gtgtctatga tgacaccttt gcgctgaaag agcttgccag aagaacagat
    26821 gtcattacct atgaattcga aaacgtcagt atagaggcgt taaatgcgat tttacccatg
    26881 tcatttattc ctcaagggac agacttgctt gcgattacgc aagaccgcct gttagaaaaa
    26941 tcttttttgg aaacgaataa catcgtgatt gctccttatg caacgatcgt cagtccgacg
    27001 gatatccaag atgcgattga cgggatcggc tatccttgtg ttttgaaaac cactcgaggc
    27061 ggctatgatg gcaaggggca gtatgttttg aaaagtcggg cggatctagc tcctgccatg
    27121 gaccttttaa gagaaggaac ctgtgtttta gaagcatgga tcccgtttga aaaagagatt
    27181 tcgatcatgg tggcaggaaa tggtcaagga gatttcacga cttttccggt cgtggaaaac
    27241 atccaccaca acaatattct tcatgaaacg attgctccag ccgcgattga tcaggatgtg
    27301 atcgaggagg ctgagcgtat tgcccgtgtg attgcagaag cagtgagcct ttcaggtgtt
    27361 ttaggggtag aaatgttttt aacaaaaaca ggcgggctat atgtgaatga attagctcct
    27421 cgtcctcaca attctggaca ttattctatt gaagcttgtt ctatgagtca gttcgatgcc
    27481 catattcgag gtatttgtgg ttggccatta ggagaaaaag tacgtttgat aagcaatgca
    27541 gtaatggtca atgttttggg agaacaactg ctggaaagct atcgcttgat tgagaataag
    27601 ccagactggc aattccattt ctatggaaaa aaacaagcaa aaaaaggtcg caagatggga
    27661 catatcacta tcttgacaga agacatccag caaacgctgg aagaaatcaa acaaacgaat
    27721 atttgggatt agaggagaaa aaacatgtta gaacgttata cacgccctga aatggggaat
    27781 atctggacgg aagagaacaa ataccgtgcg tggctcgaag tcgagatttt agcagatgaa
    27841 gcttgggcag aacttggtga aatcccgcaa gaagatgtga aaaaaattcg tgagcatgca
    27901 tcatttgata tcgatcggat cttggaaatc gaaaaattaa cacgccatga tgtcgttgcg
    27961 tttacacgag cggtttctga aacattggga gaagaaagaa aatgggtcca ttatggtcta
    28021 acaagtacgg atgtcgtaga tactgcttat ggctatctca tcaagcaagc aaatgatatt
    28081 ttacgggaag acttgaaacg ttttacggaa atcattggcg aaaaagcaaa agaacacaaa
    28141 tatacagtga tgatgggtcg gacacacggt gtccatgctg aaccaactac ttttggattg
    28201 aaacttgcca catggtattc agaaatgaaa cggaacatcg aacgatttga acatgcggca
    28261 aaaggagtag aagcaggaaa aatctcagga gcagtgggaa cgttcgcaaa tatcccgcca
    28321 tttattgaag agtatgtatg taaacaattg gggatccgtc cacaagaaat ttctactcaa
    28381 gttctccctc gggatctaca tgctgaatat ttctcagcga tggcgcttat tgctacaagt
    28441 attgaacgtt ttgcaacaga gatacgcgga ttgcaaaaat ccgaaacacg tgaagtagaa
    28501 gaattttttg cgaaaggtca aaaaggttct tctgctatgc cgcacaaaag gaatccgatc
    28561 ggttcagaaa atatggctgg tctagccaga gtggtgcgcg gacacatggt cactgcctat
    28621 gaaaatgtca cattatggca tgaaagagat atttctcatt cttcagcaga acgaatcatc
    28681 ttgcctgata ccacgatttt attgaattac atgttaaatc gttttggcaa tatcgttaaa
    28741 aatctaacgg tctttcctga aaacatgaag cggaatatgt ctgcaacatt tggtttgatt
    28801 tatagccagc gcgttatgct gaaattgatc gataagggaa tgacaagaga agaagcttat
    28861 gatcttgttc agccaaaaac tgcgtacgct tgggacaacc aagtgcagtt ccgcccgctg
    28921 ttagaagaag atccaaccat cacatcggta ttatctaaag aagaaataga cgatgccttt
    28981 gattatcatc atcatttgaa aaacgttgac gtgatattca aacgagtagg tttggaataa
    29041 agaagagtga gttgcaaaat tcaattaaaa tagaatagca aaaataacca aaaaagaagc
    29101 agatccgtta gcggttctgc ttcttttagt ttattatatt ttcttatcaa taataggaac
    29161 ctgattttaa aagcggctac ctctttaccg tgcctcttta catacaaaaa atagaggaat
    29221 taagcgtaaa tggcttggct attataacta aaatgctggt tatggagatg ttgcagcaag
    29281 ttttcgagaa aagcgtattc catctcttgt ccattgaata agatctggat acgataatga
    29341 tttttgaaac gcttctgttc atgaatctga ctgattgaaa gttcgccttt gtgttctgtc
    29401 tgataaagaa aagtcctttt acggcattca gcacagcgct tggttgctcg gcggattttt
    29461 cggaatgtat aaaaactagt atctttattg cataaataaa cgtctttgat cgacagcaaa
    29521 tcattatata gacaggaacg ttcttcagct gttaattctg ataaaaaaag ctgaccttgt
    29581 tttattgcat cttccatcgg caagtgcctc ctcgaaccca ttatccaaat ttttagttaa
    29641 ttctaatata ccatcattaa gtataaatgt aaacgcttgt tgatggtata agattttcgt
    29701 ttctgataaa acacgaacat taaaagaatc taatgcctta aatgtacata tttttgttga
    29761 tttataaaaa gatgattgct aaaatttaaa gtgaaaaaac gaacaaaaaa cgatgtattt
    29821 gcttttaatg taaataaaaa ggagagacgt acatggagaa gaaagaactt ttgtatgaag
    29881 gaaaagccaa aaaaatatat cttacttctg atgagaactt gatttggata gaatatttag
    29941 atcaagcaac tgcgttgaat ggcgcgagga aagaccagat aagtggaaaa ggagaattga
    30001 ataatcaaat cacaagtgca attttccgtt atctgactca aagaggaata aaaaatcatt
    30061 ttattgaaca attgtctaaa accgaacaat tagcaaaaaa agtacaaatt atccctctgg
    30121 aagtggttat ccgaaatatt gcagcaggaa gtttctcaaa acgtctaggt gttcccgaag
    30181 gtaaagaatt catcaaacca atcattgaat tctactataa ggatgaccag ttggatgatc
    30241 catttatcaa tgaagagcat atctatttct tacaacttgc aaagccggaa gaaatcagtg
    30301 agctgaaagc ccaagcgttg gctattaatg aaattttgac cgaattattt gctaaggtcg
    30361 gtttggtttt agtagatttc aagctagaat ttggccggtc gaatggacaa attttgcttg
    30421 cggatgaagt aacaccggat acttgccgat tatgggatga acgtacgaat gaacatatgg
    30481 acaaagacgt ttaccgcaga gatctaggtg atttgatccc agtctatcaa gaagtatata
    30541 accgtctatc ggaactaact gaagaaaagg agcgagtatg atgtatttcg taaaagtata
    30601 tgtaacctat aaagattcag tgttagatcc tcaaggggag gcagtaaaaa aagcagtcca
    30661 tcgtatggga tatgaaacaa tcgaagatat ccgaatagga aaatactttg agatacaact
    30721 tgctgatgca gaagaaccaa tcgatacagt gattgaaacg atttgtgaca agctgcttgc
    30781 aaatgtaaat atggaaacat accgatatga aatcaaaaaa atggaggaag tataacatga
    30841 aatttgcggt catcgttttt ccaggttcaa attgtgacgc agatatgtta tgggcagttc
    30901 gcgagatcat gggcgctgat gccgaatttg tacggcatga tgagtcctct ttagaaggat
    30961 ttgatggggt tttactgccg ggaggattct cctatggtga ctatttgaga tgcggtgcga
    31021 ttgctcggtt ttctacaatc atggaagaag tgattcgttt tgctgaagaa gggaaaccag
    31081 tattcggtac atgcaatgga ttccagatat tgacagaagc aggacttttg ccaggtgtac
    31141 tgagacgaaa tgagtctttg cattttattt gcaaaactgt accattgaaa gtcaacccca
    31201 agacggcctt tacttcagag tatgaagccg acgaaataat ctatcttcca gttgctcatg
    31261 gggaaggaaa ttatttctgt gacgaagaaa cacttcaaga actaaaaaaa aataatcaga
    31321 ttgcctttac ttataatgac aacatcaacg gcagcgtgga aaacattgca gggattacga
    31381 acaaagcagg gaatgtgtta ggaatgatgc ctcatccaga acgtgcagta gaagacttgc
    31441 taggatcaga agatgggaaa cggttctttg cttccatttt aaaaaacttc ggaaaggtga
    31501 ccgtatagat gaaaacattg aaagaaccga cagctgaaga gatcaagaaa aaaagaatct
    31561 atgccgaatg gggattgact gatgaagaat atcgaatgat cgaagaagat atcttaggaa
    31621 gaatgcctaa ttatacggaa actggactgt tttctgttat gtggagcgaa cattgttcct
    31681 ataaaaattc taaaccggtt ctacgcaaat ttcctactag tggacctcat gtgttgcaag
    31741 gccctggaga aggcgcaggc atcgtcgaca tcggtgatgg actagcagtt gtcttcaaag
    31801 cagaaagtca caatcatcct tcagccgtgg agccttatga aggagctgcg acaggagttg
    31861 gcggaatcat cagagatatt ttcagcatgg gggctcgtcc aattgcgttg ctagattcat
    31921 tgaggttcgg cgaattagat aatccaagaa cgaaatattt actagaagaa gtagttgcag
    31981 gtatcggcgg atacggcaat tgtatcggca taccgactgt aggcggcgaa attgcatttg
    32041 atccttgtta tgaaggaaac ccattagtca atgcaatgtg cgtaggtttg attgaacaca
    32101 aacatatcca gaaagggcaa gccgctggcg tcggtaattc gatcatgtat gtaggtgcaa
    32161 aaacaggtcg cgacggtatc cacggagcaa cttttgcctc agaagaattt aatcaagaag
    32221 aagaacagca acgttctgct gtacaagtag gcgatccttt tatggaaaag ctgctgcttg
    32281 aagcgtgttt ggaattaatc ttgcatcatt cggattctct aataggtata caggatatgg
    32341 gagcagcagg acttgtttca tctagtgcgg aaatggcttc aaaagctggc tcaggactta
    32401 tattgacatt agatgaagtc ccacaaagag aaactgggat gacaccttat gaaatgatgt
    32461 tatcggaatc acaagaacgg atgttgattt gtgttaaatc aggagaggaa gaaaaaatcc
    32521 aagaactatt tcagaagtat gatctagatg cagtcactat cggtaaagta acagacgacg
    32581 ggcaatatcg gctatatcat caaggagaag aagtagcaaa tctgccagtc gatgcattag
    32641 ctgaagatgc accagtttat cataaagaaa tgaaagaacc agctcgaatc gtagaatttc
    32701 agcagttagc tccttatcag ccagttatcg aagaacctgg acaagtattg cttgatctgc
    32761 tgcagcaacc gacgattgct tcaaaacgtt caatctacga aacttatgat tcacaagtac
    32821 aaaccaatac agttgtccgt ccaggaagcg atgctgctgt gatgagggtt agagggacga
    32881 ataaagcgtt agctatgacc acagactgca acgcgcgcta tctgtatttg gatccaaaaa
    32941 tcggaggaca aatagcagtg gcagaagctg ctagaaatat tattgctagc ggtggtaagc
    33001 ccttggcgat caccgactgc ctgaactatg gttctcctga taaaccagaa gttttttggg
    33061 aattatcgac ttcagctgac ggaattgctg ctgcgtgtga aacgttagga actcctgtta
    33121 tttcaggaaa tgtctcttta tataatgaaa cggatggtca agcaatctat ccaacaccaa
    33181 tgattggtat ggtcggattg attgaggatc ataaacatat cactactcag gaattcaaaa
    33241 aatcagggga tctaatctat atattaggta agacgtttgc agattttgat ggcagtgaat
    33301 tacaaaagat gcagcttgga agaatcgaag gtgtgatccg aaactttgat ctatccattg
    33361 aaaagagaaa ccaagagtta gtattgacag ccatccaaaa cggattgatt gaaagtgctc
    33421 atgactgttc agaaggtggc ttggcaattg cactggcaga atcagctttc aaacatcagc
    33481 tagggatatc tgtacagttt gaattgtcct ctgctcaatt atttgcagaa actcaatcaa
    33541 gatttgtgtt gacagtcgca ccggaaaata agacacgatt cgaagaaatg atgggagatg
    33601 ctgctgtttt agcaggtaag gtgacggatg aagcgataat cgaaatttca gcaactgacg
    33661 gtcagatcaa aatagaaact gcagtagcaa gaaagtgctg ggaggatgca atcgtatgtc
    33721 ttatgaagtg aagagtttaa atgaagaatg cgggatcttc ggtgtttggg gtcatccaga
    33781 cgccgctagt gtggcatact tcgggctcca tagtcttcag catcgaggac aagaaggtgc
    33841 aggtatcgtg tccaatgatc gaggaaagct taaggggcat cgagatttgg ggctgctttc
    33901 tgaggtattc aaagatcaaa gaaaattgca gcagttaaca ggagaagctg caatcggaca
    33961 tgtacgttat gcaacagcag gcaatggaag tgtggacaat attcagcctt ttctattcaa
    34021 atttcatgat cagtcgatcg gcttagcgca taatggtaac ttgatcaatg ctcgttccct
    34081 tagaaaagag ttagaggaaa atggggcgat cttccattct aactccgata cggagatatt
    34141 gatgcatttg atccgacgaa gtgaagaaac agatttttta aataaattaa aaagtgcttt
    34201 gaatgaagtc aaaggtggct ttgcctattt attaatgaca gaaacggcaa tgattgccgc
    34261 tttggaccca aatggttttc gtccattagc aatcggacaa atgaaaaacg gagcatacgt
    34321 gattgcctcg gaaacttgtg cattagaagt tattggggca cgttttataa gagatgtcca
    34381 gcctggtgag atagtgatca tcaatgacga aggaatccag attgattgct ttacgaagga
    34441 cacgcagctt tctatttgtt cgatggaata tatctatttt gccagaccag attcaaacat
    34501 cgcagggatc aatgtgcata ctgcacggaa aaatatggga cggaatttag cgaaagaatc
    34561 gccagtaaaa gcagatatgg tcgttggcgt acctaattct tctttgtcag ccgcaagcgg
    34621 ttatgcggaa gctagtggaa tcccttatga aatcggatta gtaaaaaatc aatacattgc
    34681 ccgaacattt atccagccaa cgcctgagct aagagagcaa ggtgttcgaa tgaaattatc
    34741 tgctgtacga ggagtagtgg aaggtaaaaa ggtgattatg gtcgatgatt caattgttcg
    34801 gggtacgaca agcagacgga tcgtccatct tttgaaagaa gcaggggcaa aagaagtcca
    34861 tgtgaggatt gcttctcctc ctttgaaata tccatgtttt tacgggattg atattcaaac
    34921 gcgtaaagaa ctcattgcgg caaatcacac gattgatgaa atcaaagaat gtatcggtgc
    34981 agattcttta ggttttttaa gtgaagaggg tctgatcgaa tcgatcggtc tggaaaaaga
    35041 tgctccgtat tcaggattgt gtatggctta tttcaacgga gattatccga cgccgttata
    35101 tgactatgaa gaaaaatatc aggaatcact aaaagaaaaa gtatcgttct tttaaaagca
    35161 gaccaagaag ggagtatatg aattcaatgg caaatgcata cgcaaaagct ggcgtagatg
    35221 tcgaagctgg atatgaagta gtagaacgga tcaagaagca tgtcaaaaaa acggaacgtt
    35281 taggtatgat gggcgctata ggaggattcg gcggttgttt tgatttatcg gtctttcaat
    35341 taaaagagcc agtccttgtc tcggggaccg atggcgtggg gacgaaatta gcagtggcaa
    35401 tcgaagcaga caaacacgat acaatcggga tcgactgcgt agcgatgtgt gtcaatgata
    35461 tcttagcaca aggcgcagag ccgttatatt tcttagacta cttagcaacg gggaaaaatg
    35521 atccgttacg tttggaaaat gtagtaaaag gtgtagcaga tggatgtgtt caaggaaata
    35581 tggcgctgat tggcggagaa acagctgaaa tgcctggtat gtactcaggt aatgattatg
    35641 atctagccgg atttgctgta gggatagctg aaaaatctca attattgaca ggagaaacga
    35701 tcaacccagg agatgtgttg cttggaatag catctagcgg gattcattca aatgggtact
    35761 ctcttgttcg caaaattttc tttgagcagc agcagttttc tttatcatca aaactgccag
    35821 aattgaaaaa aacacttggc gaagaattgc ttgagccaac gaaaatctat gtttcttcct
    35881 tgcttccgct gatcaaagaa aagaaaataa aaggagctgc acatatcact ggtggagggt
    35941 ttgtagaaaa tatcccgcga atgttaccag aatctatggc tgcacaaatc aaacttggct
    36001 catggccgat tcttccaata ttcggctgtt tacaaaaata tggtgaaatt cctgagatgg
    36061 agatgtatga aatcttcaat atgggcatcg gtatggtcct ggcagtcgaa gaatcgaaag
    36121 taaaagaaat agtacacgaa attgagcaag ctggcgaaaa agtttatgaa atcgggaaag
    36181 tgatcgccaa agaaaaacat cgtgtggtgt ttataggaga agaacgatga gaatcgctgt
    36241 ttttgcttct ggaaacggaa gcaactttca agcgctagcc gattatctat cgaaaaaagg
    36301 attggaatct tcgattgact ggttgttttg tgatcagcct gaagcatatg ttttgaaacg
    36361 tgcaactgcg ttgagtgtac cggcagattg tttttcacct aaagaatttg attcaaaaaa
    36421 agagtatgaa gaagcaattt tgcataaatt gaaagaaaaa aagatcgatt tgatcgtatt
    36481 agctggatat atgcgaatca ttggaccggt attgctgaaa aattatgata aaagaatcat
    36541 caatatccat ccgtctcttt tacctgcctt ccctggtttg catggcatac gtgatgcctt
    36601 tgaagctgga gtaaaagaaa cgggtgtcac aattcattat atcgaccaag gtgtagatac
    36661 tggaccaatc atccggcagg aaaaagtgag aattgaacag gaggacacgt tcgattcttt
    36721 agaagaaaaa atccatagag tagagcatcg aatctatcca gaagtgatta gtgaaattat
    36781 tgaaaatgga ggctatctga aatgacaaga gcattgatca gtgtatcaga taaaactggg
    36841 attgtagcat tcgcccaagg tttgattgaa caaggagtag aaatcatttc aacaggagga
    36901 acaaagcatg cgttggataa agctgggatc aaaacgcttt cgatcgaaga agtgacaggg
    36961 tttcctgaaa tgatggatgg ccgtgtcaaa acacttcatc ctaaaatcca tggtggtctt
    37021 ttaggaagac gcgatctccc tagccatatg gaggcaatgg aaacggaaaa tatccaacca
    37081 atcgactttg tttgtgtcaa tttatatcct ttcaaagaaa cgattatgaa accagatgtc
    37141 gaaatagcag aagctattga aaacattgat atcggtggtc ctagtatgct ccgtagtgca
    37201 gcaaaaaacc atgaatttgt cacctctgtt gttgatccat ctgactatga agaagtactt
    37261 tcagaattac gtcagtatgg gcatacttca ctttctttca gacaaaaact agcggcaaaa
    37321 gtctttcgtc atactgcggc atatgatgca ctgatcgctc aatacttaac ggattgggtg
    37381 gaagaaaaag aaccggaaaa attgacactt acgtatgaac gaaaacaaac gcttcgttac
    37441 ggtgaaaata gccatcaagc cgctgccttt tatcagtccg ctcttctgga aaaatattcg
    37501 atcgcagcag caaaacaact ccatgggaaa gaactttctt ataataatat ccgagatgca
    37561 gatgctgcgc tacggatcat gaaggaatat gacgagccaa cggtagtcgc cttgaaacac
    37621 atgaatcctt gtggtattgg gacagcatct acgattttag aagcgtggga agcagcgtat
    37681 gaagcagatc ctgtttcaat ctttggtggg atcatcgttt tgaatcgaga agtagatctg
    37741 ccaacagcag aacagatgca taagctgttt ctagaaatca tcattgcccc ttcttataca
    37801 caagaagctt tggaattact gcaaaaaaag aaaaacatcc gattactgac agtagacttt
    37861 gcgcaggaag aaaatcaaaa tcgagaagaa acggtctcag tcttaggtgg acttcttgta
    37921 caagatcaag atctggcaat agaaaatgaa gaagagtgga aagtcgtgac aaaacgtaag
    37981 ccgactccag cagaagcaaa agcattagtg tttgcttgga gagctgtcaa acatgtgaaa
    38041 tctaatgcga tcgtactggc aaacgaacgt cagacagtag gaatcggagc cgggcagatg
    38101 aatcgagtcg gttcagtaaa aattgcgatt gaacaagcgc aagagaaaat ggaaggtgca
    38161 gttttagcta gcgatgctta ttttccaatg gatgattcgg ttgaatatgc agccaaacat
    38221 ggtataaaag caatcattca gccaggagga agcatcaaag accaagattc tattgatatg
    38281 gcgaataaat acgatctagc aatggtcttt actgatgttc ggcattttag acattagaga
    38341 ggagtcggat aaatgaacat tttggtgatc ggtgcaggtg gtcgtgaaca tgcaataagt
    38401 aaaaaacttt tagccagtcc gaaggtggat acggtgtatt gtgcgccagg aaatccgggg
    38461 atgacaaaag atggtatcaa ttgtgtcgct atcagtgagc gagatcattc agcgttaatc
    38521 cattttgtta agaaagaggc tattgattgg agtatcgttg gtcctgaagt tcctttgtta
    38581 aatgggatcg tcgatgattt tatggcggaa ggattgaaaa tcttcggtcc gacaaaagca
    38641 gcagctctga tcgaaggttc gaaaagtttt gctaaacaat taatggatac ttatcaaatc
    38701 ccgacagcta aatccagaag ttttgaatgt tacgaaaaag cagcagcgta tatcaaagaa
    38761 aatggtgcgc caatcgtagt gaaagcggac ggcttagcag ctgggaaagg tgtagtcgtg
    38821 gctaccacag tgaaggaagc gctggatgcg gcagaacaaa tgttgaacca gcatcgattt
    38881 ggggatagta gcaaaaaaat cgtcgtagaa gagtttttgg caggagaaga attctccctt
    38941 ttggcgtttg tccgtaatac caaggtgtat ccgatggtca tttcgcaaga tcataagcgg
    39001 gcatttgata acgataaagg gccaaataca ggcggaatgg gtgcttattc accagtaccg
    39061 caaatttcaa aaaagatgat tgaaaaagct gtggaagaag ttttaaaacc agcggctttg
    39121 ggaatgaaag aactaggacg ctctttcacg ggtgttttat atgcgggttt gattgctacg
    39181 aaggaaggac caaaagtgat cgaattcaat gcgcgtttcg gtgatcctga aacgcaagtc
    39241 gttctttctc ggttgaagag tgactttgcg caagtaatcg atgatttatt agagaatcgt
    39301 acagtagagt taaagtggca gcaagaagga tacaatgtag gcgttgtggt tgcagctgcc
    39361 ggttaccccg aagaatatga gacaggaatg gttctaccag attttctgga agaagagctt
    39421 tctgtttact atgctggagt ttcctcaaat aacggcaaac tagttggagc aggaggacgt
    39481 ctttatttag tagaagcata cggtgagacg atcaaagaag cacagaaaaa aatttacacc
    39541 gcgttaatgg ataaaacgac agatggaaca ttttatcgta cagatattgg agaaaaagct
    39601 gccaatagcg aactgatcgt gaagtaaatc agctgcttcc atttataaaa aatacaacaa
    39661 acataatcgg aagcgccgat tttagtgaag ccttttcata aagagtgtgg tacacttttg
    39721 ttgtgtatat aaataaggag gagttcagat gaaaaaaatt caattaggta cttctgatca
    39781 gaaagcagct gcagtgatct taggctgtat gcgtatcaac aatgcggaag atccagtaaa
    39841 agtgattgag acagcttatg aaaacggcat tgattttttt gaccatgcag atatttatgg
    39901 aaacggtgaa tgtgaagaaa tctttgcaga tgctctagca aaaacatcaa tcaaacgaga
    39961 agatcttttt attcaaacaa aatgtggaat cgttccaggt gtgatgtttg atttttctaa
    40021 aaaacatatc attgaatctg tagaaaaaag tttgacccgt ctgaaaatgg attatgtgga
    40081 tgctttactt cttcatcgtc cagatacatt ggtagaacca gaggaagtag ctgaagcgtt
    40141 tgatgaattg gaacggtcag gaaaagttcg ttattttggc gtcagcaatc aaaaaccaat
    40201 gcaaatcgaa ttgctgaaaa aatatgttcg tcaaccatta gtagctaatc agctgcaatt
    40261 tggcttgaaa catactggaa tgatcgatca agggattcat gtcaatatga cagatgaagc
    40321 aagtatcgat catgacggta gtgtacttga ttattcccga ttgcatgata tgacgatcca
    40381 agcatggtca ccataccagt atggattttt tgaaggtgta tttatcggga acgaaaaatt
    40441 ccctgaatta aaccaaacat tagaagaact agcagctaaa tatcaaacga caccaactgg
    40501 cttggcttct gcttggattt tacgccatcc agccaatatg caagtgatcg ccggtacgat
    40561 gaacgtaggg cgaatcaagg aaatcgctca agcttcagaa attaatatgt ctcgggaaga
    40621 ttggtacgaa ttgtaccgtg ctgctggaaa cattttacct taaagattgt cataagcagg
    40681 tcaaaagggc tataaattaa aaagacagaa taacttgaaa gctgttttca gcttcgagat
    40741 attctgtctt tttttattgc aaatggaaag agtatcattg tacggatttc gtttattagt
    40801 tatttttgtt tttaaggaaa agctgtgtcc gatattcttt tggagaaatc ccttgttttt
    40861 tcttaaataa ataactgaaa tagttagggt cattataacc aatttcaaaa gcaatatctg
    40921 aaatacgata gtctgattgc tgcaataatt tttttgctaa ttcgattctt tgttctgtca
    40981 gataatcgat gaatgtttgg ttaaacgatt gagaaaaaat cgtactgaaa tgagagggac
    41041 ttaatgcaac ttgttgagcg acccaagtta aggacatatc tggatcggta aaattttctt
    41101 tgataaaatc caatgcatgg ttcaaaattg gctgatattt gttccttgaa ggctgtgagg
    41161 gcagagcagt taagaaagaa atgaacatat aaactgtcga acggtaaagc gagtcgtcgg
    41221 cgctgattgc taagcgttga ctggtggatg tgcaatgaac catttcttgc gggattttct
    41281 tttctttcag gcagacatgc tggagtagct tagtcaagac aaataaacga tataatcgtg
    41341 ttcgagttgg tgtgccttgt ggtttcgtca agtgttcgat gtacttagat acttcgtttt
    41401 tcgataatga agacaaatca agtgagaggt caaagtaaaa gacagaatca actggaagtt
    41461 tcgcttcttg aatcaaatcc tcatagctga taatttgttc tttaagggaa ctggcggttt
    41521 caagtatcat ctgcgtgatc tcaaaagatg caggtaattc gctgattctt tctgcgacag
    41581 gaccaatggc gacagaaaaa ttattttttt gtcccttttc taattcatgg accaacgttt
    41641 gggctacctg ataactggtc tcgatcacat tttcttcaag agaatcaact attaaaaatt
    41701 tgatgaatcg agaggaaaca gcagagaaga tcacttgttg attctcccta aataaaaact
    41761 tcaaataatc actgagatga gcgtaatctt caaagtctgt actgacttgg tttgctgcta
    41821 ataatacggt catttttttc cctgctaaag agcgattgaa agattcagca agtttcaatg
    41881 tttcactgat cgtaagtgag cctttgaaca aaccgttcaa gaaatgattt tttttcaatt
    41941 cgaaaacaag atcagaagaa gtgtgcgttt ctttttgctg caaatctaaa gcagttactg
    42001 ctttttctat tgcttgtttc aattcgctac ttttaatcgg tttaagcaga taatcatctg
    42061 cctgtacctg gattgctcct ttcatataat cgaaatcgtc gaatccacta ataaatatga
    42121 gacgagtcca aggaagtagt ttccttgctt cttttgaaaa agtcaacccg tccatgaaag
    42181 gcatttggat atcagtcaga atgatgtcgg gctgtaagtc gataattgaa gacagtgcca
    42241 tctcaccatc tccagcttcg ccgacataag tgattggata ataagaacca aggacagtta
    42301 tttgttcccg caagttattg cgaatgaggt attcgtcttc gatgataaaa actttataca
    42361 tgtttgtttc ctccaattgt tttgggagat agagggatca tcagtgtgac agttgttcct
    42421 tctcggtatt tgctgtcaat cgtgataggt ttttgatcat ttccgtaata aagaaggaga
    42481 cgcttgttta cgttgtaaag tccgtagccg atactgaaat ctgaatcgat ccctttggct
    42541 aactctttac gtatttctgc cagtttttct gcagggatgc caatcccgtt gtctataact
    42601 ttgaattgga tcatctcatc aattttgcat gtggtaatac gaataaatcc tttgtgacgg
    42661 acgtacttcg ttccatggta tacagcattt tcaatcaatg gctgcaagat catttttaaa
    42721 atagaaacag gaaaaagtgt ttctggtata tcgatcttat agtcaagcat atggccgtat
    42781 cttattttta agatgactag ataatcattg atatggctaa tttctttttc aagaggaatc
    42841 cagtcttgtc ctttgcttaa agacgtacga aaaaagccgg aaagtgcatg ggtcatagtc
    42901 agagcttctt caaagcagtc ttgttcgatc aaagaaataa tggcatccaa actgttataa
    42961 ataaaatggg gagtaatctg agcttgcaat acacgaactt cactttgtgc taaatggtat
    43021 tgtttcaaag cgttctcccg taaagactgg tttagatctt ccgccatttt attcatgcta
    43081 gcggtaagtg tttgtaattc cagtgtttcc ggcagggtta aacgaaagcc aaggttccct
    43141 gaagaaagtt gttctgacat ctcgatcagc tgattgatcg gttcttggac ctttctctct
    43201 aaaaagcgac gattcttttt cgcatagaaa aaaatggtac taagaactcc aagttccact
    43261 attgttaata gaatgagcga gcggacaagt gtcgtatttt ttttgcttgc taagtcgatc
    43321 tccacacgaa caaaatcttg taagatatct gacagcagtt gagtgacaga atctacctga
    43381 ttgatgatct cttcaatggt atccataaaa tcttttgcca agatattatt gatcagctcg
    43441 ttttgataat cttccagtgt atcgatcgta cgtagcgata catctaacgt actaatttct
    43501 ttaagtgtat ttgtattatt tttgatctct cgaatatcat tcctaagttc ttctaggata
    43561 ttttcctcat tgaactcatc tacggttact tggccagaaa tcaagtccca catactttcc
    43621 aaaacttttt gatcaacctt caaagcaatt gcatctgctt tttggatatt tgcgatcgtc
    43681 tgttgataaa tcaaaagctg ttgcgaatat aacagtatgc tcagtagcaa agggataaca
    43741 gcaaatagaa gcatggcttt gtttgcttgg gcaaaaatct gttttatgct taaatttttt
    43801 gtcatgatac ttctccagtc aataatttct ggttgggatg ttagctatat tctgctggga
    43861 aaagacagtc tcggaaataa aaatatcttt aggtaatgtt tttcctgttt gaatatttcc
    43921 aaaataacga gtaacggtat tggctacata ccagccgaca tcaggtttac actcgactac
    43981 acaattgacg attcctgagc gtagcttctc gatcatatct tcttgtccat caatagttac
    44041 aatgatcaaa tcttctccag gtttgattga tgtttctttt atcgcagcta aagcaccgtg
    44101 agtcatttca tcactatggc tgtataatac atcgatttcg gataaatcat ttgtttctaa
    44161 atatcgtttc atctggtctt ttcctttcat ccgaataaaa tcaccgacaa ttcggtgaac
    44221 aatagtcatc ggaggtttat ggtcgatggt tccatctttt tttagggttg gtttacgatt
    44281 gattgattcc aaaaatcctt cggagcgtag cttggtcggt gtggagttct ctgaaccagc
    44341 aagttctagg attctgattt cctgtttctt tgaatttgtg aagtgattgc tgacaaataa
    44401 acccgcccga tttccttctg ctttgaaact ggggccaatg tgggtgacga aaagctcggg
    44461 atcattcgtt tgaatatgtc tgtcgacaag tatgacaggg atccctgctc gctttgcttc
    44521 ttttaataca gagtcccacc cggattcttg caaaggagta aaaatgatca tatccacttt
    44581 ataggcaatg aacgtccgta tatcttggat ttgccgttcc tgattcatgt agccatttcg
    44641 gtagagaact tggtaatctt ctttttcaag cgcagtgata attgattcag tatgcttttt
    44701 ccgccagcta ctctccgttc ctgattgcga aaaaccaacg actagtgggt caggtgcttc
    44761 ctttgtatta ggtgtacacc ccatcatttg gtaagcgatt acaacaaggg ctaaaaaaaa
    44821 taaaacacgt ttcactttaa atcactccat ctggatttgt tagtactatc ttatcagata
    44881 aaagggaaaa tcaaagtcaa aaggaatcaa tcgaaaaaaa tccaagaaac ttaaaaaaat
    44941 ccaagacaaa aaaagtacac ctaaaaaatt tatagtaaat cccataaaaa aatcaatgtt
    45001 aacgctttct ttattagcta tactcggtgt atcaattgaa atgggggata aaaaatgaaa
    45061 aacaaattta gaaagattgt tgggactatc gctattttga ccgcaagcat tctattaagt
    45121 gcgtgtggca attcaggatc tgcagatgat agtacggggt atgtaggaat cgctatgccg
    45181 accaaatcag cagaaagatg gatcgctgat gggaacaaca tggtctcgga attagaaaaa
    45241 ctgggttata aaacagatct tcaatatgga gaagataaag tagaaaatca agtagcacaa
    45301 atagaaaata tgattacaaa aggggtagat acacttgtga ttgcttctat cgatggttcg
    45361 gctttaactg atgtgttagc taaagcaaaa gaagcagata tcaaggtgat cgcctatgat
    45421 cgtttactga tgaattcaga aaatgtcgat tattacgcga cttttgataa ttttggagta
    45481 ggtgtatcgc aagcagctta tattgaagag cacttaggat tgaaagaagg aaaaggacca
    45541 ttcacgattg aattatttgg tggctcgcct gacgacaaca acgcactaat caattacaac
    45601 ggagtgatgt ctgttttaca accatatatg gacaacggtc agttggtcgt gccatctgga
    45661 cagacgagtt tcagtcaaat cgctacgctt cgctgggatg gttctacggc gcaagcaagg
    45721 atggataatc tgttaagcgc aaattatact gaccaaacat tggatgctgt tttgtcgcca
    45781 tatgatccaa taagtttagg aatcatttcg tcattaaaag gcgtggggta tgggagtgaa
    45841 agtaaacctt tacctgtgat tactggacaa gatgcgacag ttgctggcgt taaatcgatc
    45901 attgccgggg aacaaacaca aacgatcttt aaagacacac gtatcttagc aaaaaatacg
    45961 attgaaatga tcaaagcaat cagtgatgga gaagaggttc cggtcaatga tactgaaact
    46021 tatgataatg gggtgaaaac tgtccctacg tatttagcaa atacagtatc tgtcgataaa
    46081 gataattatc aagcagagct gatcgacacc gattattata aagaatccga tctgaaaaat
    46141 taagaggaac caaacaagga actaaagaga tctaagtcag agagaggata agaattatgg
    46201 cggattgtat tttaaaaatg gcagatatca ccaaaaaatt ttctggaatc cgagcgttag
    46261 attgcgtgaa tctgatgatt gaacgtgggg aaatccatgc attgtgcgga gaaaacggag
    46321 cgggaaaatc aacattgatg aatgtcttga gcggcttgta tccatatggc tcctatgacg
    46381 gggaaatctt ttatgatgga aagctgtgtc aatttaaaga tttgaaagat agtgaagaaa
    46441 aaggcatcgt catcattcat caagaattag cactaagtcc ttatctttca ataaaagaga
    46501 atatttttct cggaaatgaa caagcagaaa gaggcgtgat tgattgggaa ctgacagaaa
    46561 agaaaacata tgaactgctg aataaagtcg gtttgtctat cgacccgaat cttctagtat
    46621 cccagattgg cgtagggcaa cagcaactag ttgaaatagc taaagcatta tcaaaatccg
    46681 tccgtctgtt gattttggat gagcctaccg ctgcattgaa tgaagaagaa agcgcgaatt
    46741 tgctgagatt gatccgagag ttgaaagagc aaggtgtaac ctccatcatt atttcacata
    46801 agctgaatga aatcgtggat atcgcagatc gaatcaccat tttacgggat ggacagacga
    46861 ttgagacact agaaaatgaa atgatcagcg aagagcatat tattcgcgga atggtgggca
    46921 gagatttagt tgatcgttat cctgaaagaa aaccggatat tggagaagtt tattttaaag
    46981 tagaagactg gacggtctat caccctcttg atcacgagcg aatcataaac gaccacatca
    47041 acttgcattt gaaaaaagga gagattgtag gaatagctgg actgatggga gcgggcagaa
    47101 ctgaattcgc tatgagtcta tttggacatt cttatggaag caagatatcc ggacgtatat
    47161 taaaggaagg ggcagagata tcagttaaga ccgttccttc agccattgca aacggactcg
    47221 cttatgtctc ggaggaccga aaatctttag gactgaatct gctgatggat attcgggaaa
    47281 acgtgacgct gagcagtttg gataaaataa gtcaaaaagg agtattagat aaagaaaaag
    47341 aagtaactga ggcagaaaat ttccggaaaa agatgcgtat aaaaacaaac tccatccatc
    47401 aaattgtcag tagtcttagt ggtgggaatc aacaaaaagt cgttttatcc aagtggctga
    47461 tgacacaacc cgatattctt tttttggatg agccaacccg tgggatagat gttggggcaa
    47521 aatatgagat ctacacgatt atcgaagaaa tggcagcaaa tggcaaagct gtctgtctta
    47581 tctcttctga acttcctgaa atattaggga tgtgtgatcg aatctatacg atgaacgaag
    47641 gacgtattac aggagaagtc tcgcgagaag atgcagatca ggaagtcttg atgaaattaa
    47701 tgacgaaaga agaggtgggt taagttggaa caggcaaaaa atcttacgga taaaaaagag
    47761 aaaaaactaa atttgaaaag tagagcagct gatctttttg gtcgctacag catggtgatc
    47821 attttagcgg gactacttat tgcgtttcaa ttgatgacgg atggtatttt ctggcgtcca
    47881 ttgaatataa ctaaccttgt tctgcaaaat agccatatat tagtactagc tgcagggatg
    47941 ttattagttg tgctcttagg tcatgtggat ttgtcggtag ggtcagtgat ggcgtttgtt
    48001 ggtgcaattg cgggcatgat gatggtcaat cgccaaatga acccgtggat gacagttatc
    48061 gcatgtcttg cagtaggcgg gctgatcggc gcatggcaag gtttttgggt agcttatgca
    48121 gggataccgg cgtttatcgt gactttagca ggattgctga tgtttcgtgg actgacacaa
    48181 gttgttctag gaggccaatc tctagcacct tttccagctg ttttccaaaa aatgtctacg
    48241 ggttatttac cagacttaag cgaaggaagc agccttcact tcttgacatt gattttagga
    48301 atgatcatca cgatcgtgtt agttgttggt caatggcgaa caagaaatcg tcggaaaaat
    48361 catttatttg aagtggaatc tccaacagct ttcttggtaa aggcggtatt tacagcagtc
    48421 gttgtactag gaataactta tatctttgcc tcttatcaag ggtatccgat tattttgatc
    48481 attcttggtt tgatcgttgc agcatatggg tttttgacaa ataaaacagt agcaggtcga
    48541 cagatttatg cgacgggcgg aaatcgaaaa gcggctgaac tatcagggat caaaacaaag
    48601 cgaatcacgt tctgggtatt cgtcaacatg ggagtcatgg cagctttagc tggattagtt
    48661 ttagctgctc gattgaatgc ggcaacacct caagcaggta catcgatgga actggatgca
    48721 atggcggcag tatattttgg tggtgcttcg acttccggcg gcattggaac aatcatgggt
    48781 gcgattgtcg gtggcttggt catgggtgta ttgaacaatg gcatgtcgat tttaggcgtc
    48841 ggggtagact ggcagcaggc aatcaaagga ttgatcttgc ttttagccgt tgtgctagac
    48901 ttgtataata agaaaaaacg aacaacgtag gagaacgccg gttattcaga aaaaagactg
    48961 tgacaagaca tttgtcacag tctttttttc gctaaaagcg aacattcatt gtttcgtttg
    49021 tatagactaa aaataatata tgttttttta ccattcgtat aaattctctt tttgctcttg
    49081 atttttttgc ttaattgagt taaattacat agggcgctaa aaaaagcctt gattaaatta
    49141 ataatgttcg tgttttgctg gatgtgaaca tcaggaggat aatgtaatga aagtatttga
    49201 ttacgaagat attcaattaa ttcctaataa atgtatcgta aatagccgtt ctgaatgtga
    49261 tacaacggtc acattaggta aacatacatt caaaatgcca gtagtgcctg ccaacatgca
    49321 gacgattatt gatgaaacaa tcgcagaatt cttagcggaa aatggttatt tctatatcat
    49381 gcatcggttt gatgaagcag ctcgaattcc gtttatcaaa aaaatgaaaa agcgtggatt
    49441 gattacctcg atcagtgttg gcgtcaagaa agaggaatat tcctttatag agaagttggc
    49501 agaagaatcg ctaaatcctg attatatcac tatcgacatc gcgcatggac atgctaactc
    49561 agttattgat atgattcagc atattaaaaa atatttgcct gaaacttttg tgattgccgg
    49621 taatgttggt acaccggaag ccgtccgtga attggaaaat gcgggcgcag atgcaacaaa
    49681 agtcgggatt ggtccaggaa aagtatgtat tacaaaaatc aagacaggct ttgggactgg
    49741 aggctggcaa ctggcagctc ttagatggtg tgcgaaagca gctcgcaagc cgatcatcgc
    49801 tgacggtggg atcagaacac atggcgatat tgcaaaatct gtccgttttg gtgcgacaat
    49861 ggtcatgatt ggttcattgt ttgccggtca cgaggaatct cctggcgaaa caaaagtcga
    49921 aaatggaatc gtgtataaag aatactttgg ttctgcttca gaattccaaa aaggcgaaaa
    49981 acgaaatgtt gaaggaaaga aaatctggat ccaacacaaa ggatctttga aagatacact
    50041 agtggaaatg caacaagacc ttcaatcatc tatttcttat gctggaggaa gagatctgga
    50101 agccattcga aaagttgact atgtcatcgt gaaaaattcg atttttaatg gagacacaat
    50161 ctaaaaaata aataaaatat taggatgttg tgaaaaagtt gtcctgcatt aagtaataag
    50221 aacagccaaa caaaaatagt tctttatatg ttttgtttgg ctggaattat tatcgcagat
    50281 acaagctttt gaacatcatt attcagaata agggactgtg ataagatttt tgtcacagtc
    50341 ccttatgttt tgcaaaaaaa gtttttcttt tttctcgtaa attcggtaaa atcaaaagaa
    50401 aaaatagaca atagaaaaga gaagcttatg tgggaaaaag tagagaaaaa acaaggaccg
    50461 atttatcgtc aaattatgga acagattatg agagaaatcg aaaacggaag gatcaatcct
    50521 ggaagtcaac ttgaatcaga acgaaaacta gggcaacttt ttggtgtgaa tcgttccacc
    50581 gttgtccatg cattggaaga gcttagggag cttggggtac taacgagcaa acgtggcagc
    50641 ggatggtttg ttaatcagac tgaatggggg aaattcgctg ttccaagaat cgattggcgg
    50701 caaatgatct ctcctcgtta tgagcagacg gactggtatg agcaaaaaat aagagaagca
    50761 aagcagatcc aaaaaacatc gtttcttgat ttatacgcta gcgagatgcc agatgagctt
    50821 ttgccgaatt ttgagtttcc ttcttattct cttgaagaaa tcttagcgga agaaaaagaa
    50881 atggatcttt taggctatac tccgttacgt aacaaaatca aacggtattt ggaaagtaaa
    50941 tttgaattgg cattttcccc caaccagctt cttgtgactg gtggcggtca gcaagcaatc
    51001 tttttgattt tacaaacgat tctttcagct ggagagggga tagctgtaga gtcaccttct
    51061 tttttttacc gattacctct tttcaaagct tcaggaacgc gtttatttgg gataccgatg
    51121 gatgaagaag ggatcgactt agaaaaactg aaagaatcga tccataaaaa taaattgaaa
    51181 gcagtgctta tcaacccgaa ttttcaaaac cctacaggaa aagtgatgag tcaaaaacga
    51241 cgagaaaagc tagtagaatt gtgtcgacag tatcggcttc caattattga agacgatgta
    51301 tttacggatc tttcatttct aaaccaagaa gaagtccttc ctatacgaac gattgatccg
    51361 gataatgtgt tatacgtcgg ttcgttatct agggttttag gaaaaacaac gaaaatcggt
    51421 tgggtgatag gacctgagac ttttatctcg caactagctc aagcgcaaga aatgatggag
    51481 ttttcgatga atatccttac acaaatcaca gtggctaatg tatttaatga agcaattgat
    51541 ttgaaaatgc aagatgtaag aaaaaagttg aaagaaaaaa gccgactgat aagcgcttgg
    51601 ggagattcac aatcgttttt caagcttcat tcaattttag gtggttacta tgcctggatt
    51661 acttgggatg ggaaaaaact agaccccgaa ctagcagaaa aagtagtaga tatgggtctt
    51721 ggaattgcac caagtctact ttttgggcaa gatataaatg gaatcagaat caattacagt
    51781 aggctggatg aaaaccaaat accgttgttt tattcaaaga tgaaaattct agaaaagtgg
    51841 ttaacagaat aatttcacaa agtaaataga ttaaagaata taacagcttg gatttaaatt
    51901 tcaagctgtt tctttttttt cggaaaaaga atacgatata aagaaactaa gaaaaaacca
    51961 ttatttgtac gtccgttcac gatgtgtaaa taaaataaat gtttgcaaca aaactgtata
    52021 acaccaacgt ttttaaatag aaacagatgg ttgtaagttt gattagaaaa tagaaaaaat
    52081 gcagatgaaa tataaaacgc tttccttatt gtgattttat tcactaatgc tgttaatatg
    52141 cggattggca taatttcctt tcatcgtgaa aatttgtgct tttagcttgt atttctcata
    52201 aatccatgat aatttattaa tgaagacggt taaatagtga tacagtttta ttcactgttt
    52261 caatcgcaga atgaagagat tttcttatag atgagagagg aatgtgtgac aaatggcaaa
    52321 caaaaaagca ccgatcgact tccaagcact gctagatgaa gtcaatgcag atttcccagt
    52381 atatcaagca cttgatcaag atggaaacgt agttaacaaa gatttagtac ctgatttatc
    52441 cgatgatgaa ctcgttgaat taatgactca tatggtatgg tctcgtgtat tagaccaacg
    52501 ttcaacagcg ctgaaccgcc aaggacgcct aggctttttt gcaccaactg ctggacaaga
    52561 ggcaagtcag ctggctagtg cttacgcatt tgataaagaa gatgttcttc ttccaggtta
    52621 tcgggatgta cctcaactag taaaacatgg tttaccttta tctcaagctt ttttatggtc
    52681 tcgaggacat gctgcaggta attcttatcc ggaagaattg aaagctctac caccacaaat
    52741 tatcatcggt gcacaatatg tacaagcagc cggtgttgca ttaggtttga agaaacgaaa
    52801 caagaaaaat gtcgtcttca cttatactgg tgatggcggt tcatcacaag gtgattttta
    52861 cgaagcaatc aatttcgctg gcgcttacca tgcaaatgcc gtattttata tccaaaacaa
    52921 cgggtatgcg atctctacac ctcgtgaaaa acaaactgca gcaaaaacac ttgcacaaaa
    52981 agctgtcgca gctgggattc ctggtattca agtcgatgga atggatccgc ttgcagtata
    53041 tactgtatct aaaatggcac gggaatgggc gatcagtgga aatggaccag tcctaatcga
    53101 aacactgact tatcgttatg gaccacatac actatctggt gacgatccaa cccgctaccg
    53161 tacaaaagac acagatgacg attggcagaa aaaagatcca ttgatccgct tccgtaaata
    53221 tttgacggaa aaaggacttt ggtctgaaga aaaagaagaa caagtcatcg aagcaactaa
    53281 agaagaaatc aaagcagcta ttgctgaagc cgacaaagta ccaaaacaaa aggtttcaga
    53341 tttcttgaaa aacatgttcg aagtatctcc acaatcaatc aaagaacaaa ttgcaattta
    53401 tgaagcgaag gagtcgaaat aatcatggca caaaaaacaa tgatccaagc aatcacagat
    53461 gccttagcac ttgaactaga aaatgacgag aatgtcgttg tttttggtga agatgttggg
    53521 aaaaacggag gagttttccg ggcaactgaa ggattgcagg aaaagttcgg agaagaccga
    53581 gtattcgata ctcctttagc tgaatctgga atcgcaggat tgagtttcgg tcttgcgtta
    53641 gaaggatttc gtccagttcc tgaaatccaa ttctttggtt ttattttcga agcaatggac
    53701 gaagtagtag ctcaaatggc tcgtacaaga tatcgaatga gcggcactag aaatctaccc
    53761 atcacaattc gttcgccatt tggtggcggt gttcatacac cagaattgca ttcggataat
    53821 ttagaaggtt tgatcgctca gtctcctggt attcgagtag tgattccttc caacccttat
    53881 gatgcaaaag gactattgat ttcttctatt agaagtaacg atccggttgt tttcctagaa
    53941 cacatgaaac tatatcgctc attccgagaa gaagtgccgg atgaagctta cgaagttcct
    54001 ttggataaag cagcagttac tcgtgaggga acagatgtat ctattatcac atacggagca
    54061 atggtgcgtg aagcaatcaa agcagcagac aatcttgcaa aagaaaatat atctgttgaa
    54121 atcattgatc ttcgtacagt tgcgccatta gatgtagaaa cgatcattca atccgtggaa
    54181 aaaactggtc gcgtagttgt tgtccaagaa gcacaacgac aagcgggcgt tgccgcgcaa
    54241 gtagtttctg agatctcaga acgcgctatc ctttcattag aagccccaat cggacgagtt
    54301 tctgcacctg atacagtctt cccatttgga caagcagaaa atgtctggtt gccaaatgca
    54361 aaagatatcg aagacaaagt aaaagaaatc gcggaatttt aagaaaagaa gggacgaaac
    54421 aaagtcatgg cttatcaatt taaattgccg gatatcggtg aaggaatcgc agaaggtgaa
    54481 atcgtcaaat ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa
    54541 gttcaaaatg acaaatcagt agaagaaatc ccgtcacctg ttacaggaac ggttaaaaat
    54601 gtgattgttc cagaaggtac cgtagcaaat gttggggatg tattagtaga aatcgatgca
    54661 ccaggacacg aagacaatga aggggacagt ggtgtagccg cagaatccca aactccagct
    54721 aaaccggctg ctgaacccac tgttgataca gaatcagctg gatcgtcaag cgaaggagtc
    54781 ttccaattta agttaccgga tatcggtgaa ggaatcgcag aaggtgaaat cgtcaaatgg
    54841 ttcgtcaaac caggagatac gatcaatgaa gatgatacat tactagaagt tcaaaatgac
    54901 aaatcagtag aagaaattcc gtcacctgtc acagggacag ttaaaaacgt gattgttcca
    54961 gaaggcactg tagcaaatgt tggggatgta ttagtagaaa tcgatgctcc tggacacaat
    55021 agtgcaccgt caactagtgc gccatcagct gaagcaccaa aagaaaaagt ggaaacatca
    55081 ggttcagcaa gtgtcgttga agctgccgat ccaaataaac gtgtattggc tatgccatct
    55141 gttcgtcaat ttgcccgtga aaaagatgta gatatctcac aagtaactgc aactggaaaa
    55201 ggtggcagag taacaaaaga ggacatcgaa aacttcttgg caggtggtcc aagcagtgca
    55261 ccagcgaaat cagaagcgcc agaagcggca gcaccgaaag aagcagctcc tgcagcagaa
    55321 agcaaaccag ctgcaccagc aaaaccattc aagtccaact taggagactt ggaagaacgt
    55381 gtagcaatga cgccaacacg taaagctatc gctaaagcaa tggtcaacag taagcataca
    55441 gctccacacg tgacattgca tgatgaagta gaagtgtcta aactatggga taatcggaaa
    55501 cgatttaaag aagttgctgc tgcaaatggt acgaaactta ctttcttgcc atatgtcgtg
    55561 aaagctttaa cagcgactgt taagaaatac ccagtattga atgcatctat cgacgatgca
    55621 aaccaagaaa tcgtttataa acattactac aatatcggta ttgcaactga tacggatcac
    55681 ggactatatg taccaaatgt gaaagatgca gaccgcaaag gaatgtttgc tattgcagac
    55741 gaaatcaatg agaaagcaaa attagctcat gacggcaaac tatctgcaga agatatgcgc
    55801 aatggaacaa ttacgatcag caatatcgga tctgtaggtg gcggttggtt tacaccagtc
    55861 atcaactatc cagaagtagc tattttaggt gtaggaacga ttgcacaaca accaatcgta
    55921 aatgctgaag gcgaaatcgt tgtcggtcgt gtgatgaaac tgtcattaag tttcgatcac
    55981 cgtatcgtag atggagcaac tgcgcaacaa gcaatgaata atatcaaacg tcttttagct
    56041 gatccagagc tattaatgat ggaaggatga taaacaaatg gtagttggag attttgctgt
    56101 agaattagat acagttgtaa ttggtgcggg acctggtggc tatgtagctg ccatccgcgc
    56161 tgctgaaatg ggacaaaaag tagcaatcat tgaaagagag tatattggcg gtgtttgttt
    56221 gaatgtcggc tgtattcctt caaaagcact gatagctgca ggacaccatt atcaagagtc
    56281 actagattct gaaatgtttg gagtaactag tgaaaacgtc aaactagatt ttgcaaaaac
    56341 acaagaatgg aaagaaaaca aagttgtcca tacgttgact tctggtgttg gtttcctatt
    56401 aaaaaaacat aaggttgaaa caatcgaagg agaagcgttc ttcgtagatg atcacacact
    56461 acgcgtcatt catccagatt cagctcaaac ttattcgttc aataacgcaa tcatcgcaac
    56521 aggttcccgt ccaatcgaga ttccaggatt taaattcgga ggacgtgtgt tagattctac
    56581 tggcggatta gcattgaaag aagtacctaa gaaatttgtg atcatcggtg gcggtgtgat
    56641 tggtgccgaa ttgggcggag cgtacgcaaa cttaggagca gaagtaacga ttcttgaagg
    56701 ctcaccacaa attttaccaa cttacgaaaa agatctagta aaattagtag aagatgattt
    56761 caagaaaaaa ggcgtaacag tcgtaacgaa tgcaatggcg aaagaagcag tcgataacgg
    56821 tgacagcgtg acagtgaaat atgcagtgga cgggaaagaa gaatccgtta ctgctgatta
    56881 tgtgatggtc actgtaggtc gtcgtccgaa tacagatgat atgggcttag aacaagcggg
    56941 tgtcgaagta ggagaacgag gattgatcac ggtggataaa caaggacgta cgaacgtacc
    57001 taacatctat gccatcggtg atatcgttcc aggggcggca ttagcacaca aagccagtta
    57061 tgaagcaaaa atcgctgctg aagcaatctc tggcaaaaaa gtagctgtag attataaagc
    57121 aatgccggca gtagccttca ctgatcctga attggcttct gtaggtatga cgatcaaaga
    57181 agcaaaagat gctggtatcg aagcaaaagc atataaattc ccattttcag ggaacggtcg
    57241 ggcattatcg cttggtaaaa cagaaggatt catccgtcta gttacgacga tcgaagataa
    57301 cgtgcttatt ggcgcgcaaa tcggtggagt cggcgcaagt gatatggtct ctgaattagc
    57361 tttagcaatc gaatcaggaa tgaatgctga agatatcgcc ttgacgattc atcctcatcc
    57421 atctcttggc gaaatcgtca tggatgcttc ggaattagca ttaggtttac caatccatat
    57481 ttgatttgaa taatacaggc gaaacgcaac tgaaaaatca gttgcgtttt gttttttaga
    57541 attatcaata atattattat gtaaacttaa aaatatgaca acttcttggt ttattaccac
    57601 ttcctatgct acactgaaga ggaatatgaa ataggagagt tgaagatgca agaatatcag
    57661 tatccacttg atttagattg gacaacagac gaaatggtca ttgtaatgaa gatgtgggaa
    57721 gatctggaac gtgcgaatga aaaagggata aacaatgaag cttttttgaa gacttatcag
    57781 cagtttaaaa ctgttgtcaa atccattggt gaggaaaaac gattgggacg ggaatttgaa
    57841 aaagcttccg gttattcttt atatcgtaca gtgcagcaag caaaaaaaaa tccaaacaaa
    57901 actttgagaa tgaacgggtg agataaatgg aacagatgat tgatcatatc aagtcctggc
    57961 ttgaagaagc tggtgagaaa atcaaatctt ctcaacaaac attaacaatc tcgcaaaaat
    58021 caaatcgaac ggatctagta acaaatgtcg atcgaaacat ccagaatttt ctaatcgaaa
    58081 aaatccattc ctttgatcca gaggcgaaaa tattggcaga agaagaaggg tgtaatcaat
    58141 tatcggatct cgatggaaga gtctttatca tcgatcctat tgatggcacc ttgaattttg
    58201 tgattgaagg ggaaaatttt tgtattatgc tcgctgttta tgaatccggc gttggaaagc
    58261 ttggtttcat ttatgacgtg atgagaaatg aacttttttg gggtggaaaa ggaataggcg
    58321 tgtataaaaa tgatgagcca ttgcctagac cagcaaataa atccttatcg gaaggcttac
    58381 ttggtgtgaa tagttatctt ttcggtcata ataaatatgg aatccgagaa attgcggaaa
    58441 gcagcatggg gacacgtatg tacggatgcg ctggtcttga actagtggca atcttgaaag
    58501 gaaaccacat tgcttatatt tcaaacctta gcccttggga ttatgcagcg ggaaatgtct
    58561 tattggaaga gtttggtatg gtaacaagcg gtctttttgg tactcctttg acatttgaaa
    58621 aaagggaata ctatttagca gggacttctt gtgcatatgc agaaatcgca gaccttttga
    58681 aaaaagaagg ctgaaaaaaa ggtagaaatc attcacaaaa atgataaaaa cgagcaaaag
    58741 attaattttc tcgaaaaaat gaaaacattt tactatattt catgaaaaaa gtctgctata
    58801 attagcagtc gagtaaaatt tgccctgtca taaagaacca ataaaaaagg agttagttac
    58861 attgaattat agaaacgata tccgtaacgt cgcaattatt gcccacgttg accacggaaa
    58921 aacaacatta gttgatgaac tattaaaaca atcagataca ttagacgcac atacgcaatt
    58981 gcaagaacgt gcgatggact caaatgcgct agaaaaagaa cgtggtatca cgattttagc
    59041 caaaaacaca gctgtagact ataaaggtat tcgcgtaaac atcatggata ctccaggaca
    59101 cgcggacttc ggtggcgaag tagaacggat catgaaaatg gttgacggcg ttgtgttggt
    59161 cgtagatgcg tatgaaggaa caatgcctca aacacgtttt gtattgaaaa aagcactgga
    59221 acaacatatc acaccaatcg ttgtcgtaaa caagattgac aaaccttctg ctcgtcctga
    59281 gcatgtcgta gatgaagttc ttgaactatt catcgaatta ggagcagatg atgatcagtt
    59341 agactttcct gtgatttatg catctgcact aaatggtacg tcaagcttgt cagatgatcc
    59401 agcagatcaa gaaccaacca tggcacctat ttttgataca attattgaaa aaatccctgc
    59461 tcctgtcgat aacagcgacg aaccattaca attccaagtt tcattacttg actacaacga
    59521 ttatgtaggt cgtatcggaa tcggacgtgt atttagagga acaatcaaag taggagacca
    59581 agtagcatta attaaattag acggaacagt taagaaattc cgtgtaacga aattatttgg
    59641 tttctttgga ctaaaacgtc tagagattca agaagcaaaa gctggagact tgattgctgt
    59701 ctcaggtatg gaagatatct ttgtcggtga aacagttact cctgtggatc atcaagatgc
    59761 gttacctatc cttcacattg acgaaccaac attgcaaatg actttcttag ttaataattc
    59821 accatttgcc ggtcgagaag gtaaattcgt aaccgctcgt aagattgaag aacgtttgat
    59881 ggcagaactt caaacagatg tatccttacg agttgaacca acaaactctc cagatgcatg
    59941 gacagtttct ggtcgtggag aattgcattt gtctatcttg attgaaaaca tgcgccgtga
    60001 gggatacgaa ttgcaagtat ctcgtccaga agttattgaa aaagaaatcg acggtgtaaa
    60061 atgcgaaccg tttgaacgcg tgcaaatcga tacaccagaa gaatatatgg gtagtgttat
    60121 cgagtccttg agcttacgta aaggtgaaat gcaagatatg gtccatacag gaaacggaca
    60181 aatccgtttg acattcttaa cacctgcccg cggtttgatt ggttattcaa ctgaattcct
    60241 ttcaatgaca cgtggttacg gaatcatgaa ccatactttt gaccaatatc tgccaatgct
    60301 tcctggtcaa attggcggac gtcaccaagg ggcgcttgtt tcgatcgata caggaaaagc
    60361 aacgacttat tctatcatga gtatcgaaga acgcggtaca gtcttcgtag aaccaggtac
    60421 agaagtatat gaaggaatga ttatcgggga aaacagccgt gataatgatt tgactgtcaa
    60481 catcacgaaa gcaaaacaaa tgacaaacgt acggtctgct actaaagacc aaacatctgt
    60541 catcaaaaaa ccaaaacaat tgacgttgga agaatctttg gaattcttga atgatgatga
    60601 atattgcgaa gtaacaccag aaagcattcg cttgagaaaa caaattttag agaaaaatgc
    60661 tcgtgaaaaa gcatctaaaa agaaaaaata atctgtaaaa ggatgaagag agaaaccagc
    60721 ttgttataaa aaatagctgg tttctttttt tgtccaactc ttttctgtgg agaagtcatc
    60781 tttgtcaatg gacataaatt cagaaaatac gccgaaaggc gtttaaaaga cttttaaaaa
    60841 acttatttct agagagtttt tgtttcataa taagataatg tttcacgaaa ggagatcaac
    60901 ttgtccttcg aagaaaatcg aagtaaaatg gcaagagaaa ggagcgggaa gatgaacgac
    60961 ttattaaaac aatactttgg ctatgatgag tttagacctg gtcaaaaaga aattatccag
    61021 aaagtcattg atcaggaaaa tgtattagga atcatgccta ccggaagtgg gaaatcaatc
    61081 tgttaccagc ttccagcttt actattagat gggcttactg tggttgtgtc ccctttaatc
    61141 tctttgatga aggatcaggt ggacgcggcg aatcaattag gtatacccgc cacttttatc
    61201 aatagctcgt tagatggtta cgaaacagct agacgcttcc aagaaatcga tagacagcag
    61261 taccgtttac tttatatcgc tcctgaacgt tttatcatgc cggattttat ccaagcaatg
    61321 aagcgatgga acgtccgtat gatcgctatt gacgaggcac attgtatctc tcagtggggg
    61381 catgatttcc gcccaagtta tttacagatg gcgaaccagc tagatcaact gccaaatcgt
    61441 cctgtaattg ttgctttgac agctacagcg actgtgcaag tagctgctga cataaaaaga
    61501 cttttaaaaa ttccagaaaa caaccatatt cagactggtt ttgaacgaga aaatctacga
    61561 tttcaagtaa ttaaagatca aaaaaaagaa caatatctaa tcgaatattt aaagatcaac
    61621 aaaaatcaat ctggtatcat ttatgcagca acgagaaaag aagtagaccg tctctatcat
    61681 ttgcttaaaa aattcgattt ttccgtgggt agatatcatg gtggtctaaa tgagaatgag
    61741 cgaacagaaa tgcaagaagc ctttttatac gatcgcttgc agctgattgt agcgacaaat
    61801 gcatttggta tgggtataaa taaaagcaat gtccgttttg ttatccatta tcaaattcct
    61861 ggctctctag aagcatacta tcaggaagct ggtcgggcgg gaagagatgg tctttccagt
    61921 gaagccatcc tgcttttctc tcctcaagat atccaagtcc aaaagttttt tatccagcag
    61981 tcccaaagag aagaagggca aaagcaaaaa gaatatgaaa aattaaaagc gatgacagaa
    62041 tacgtgtata ttgagtcctg tcttcagcaa tacatcctaa actattttgg cgagacaagc
    62101 agtccgtgca accgttgtgg taactgttta gatgacagag aaatagtaga agtaacgaca
    62161 gaagcacaga tggttctttc ttgcttaaaa cgtatgggtg agaactatgg taaacaaatg
    62221 ctgatgaaag tcttagcagg atcgaaagaa caaaagctgc aagctttagg ttttggacat
    62281 ttatctacct atggactgat gaaaaaccaa tcgcaaaaag aaacgatgca gttaattgaa
    62341 tatttgatat cgaatggcta tcttctgacc atcaatggtg agtatcctgt tttgaaagta
    62401 acagaaagag ggatacaagt attgaaagga caagaatctg tttatcgaaa ggaacccaag
    62461 aaagtccagc agctatcgga tgaagaaacc gatacactat ttgaagtgct gcgggaactg
    62521 cgaacggatc tagcatcaga agcaggcgtt cccccgtatg tcgtattttc agattccaca
    62581 ttgaaagaaa tgagccgaat acgtccatca agcagattgg agatgcttca gataaaagga
    62641 gttggccaaa gcaagcttga taaatatggc gaagcctttt tatctcggat aaaaaatgcg
    62701 gactctaacg ttctggataa gtagaaagaa cgaatacgta aaaatgaata aaaacaactg
    62761 gttaaatctg ttttttttgc tccttatgaa aatcaaatat gttataatta aggaattaga
    62821 atgggaggta agcacagatg gattctttgt cgaaaaaact tgcgatccaa gtattaaatg
    62881 atgaagcaga acggatcaaa cgattaatcc gaaatcaaaa caacagtctc tgtatctcac
    62941 aatgcaaagc cttcgaagaa gtggtagata cacagatgta cggcttttcc cggcaagtcg
    63001 gttttgctgt ccgtatagga gtaatcggaa agaacgaggg ccagctgatg ttaagtgagc
    63061 tagaacgaga attgaatcag ttatatagtg aggtttacca agaaatttat gataaaaaag
    63121 aagttggcaa ggaggaatag acttgcctaa gaaagtgaaa aaaaggcatc tattagacta
    63181 tagcatcttg attccctatt taattttatg tgtattggga ttgatcatgg tatatagttc
    63241 tacgtcgtat ctattattag aaaacggaca aaatccgtcc gcatcagtca ttaaccagag
    63301 tattttttgg gtgcttagct tgattgctat cgcactactt tataaaatga aaacagatgt
    63361 tttgaaaaac cagcgtttga tcatggcagc catcgcagtt ttgacgattt tgctgctgat
    63421 tgttgtgttt tttggtaagg agatcaatgg ggcgaaaggt tggctgcaaa ttgcaggatt
    63481 ctcgatccaa cctgccgaat acctaaaaat catttctatc tggtacctgt ctctgacttt
    63541 atctaaaaga caaaacagtg tacaaaaaga ttttcttggg actgtgaaac gtccacttgc
    63601 aatggttatc ggacttactg cattagtcgc tattcttccc gattttggta atgcggcagt
    63661 tatctttttg attattttgg ttctgctttt agccagcgga gtcaattatg tgtatacttt
    63721 gattgtagga gtaggaggct tttgtctaag tacttttacg atctggctga tcaatatcac
    63781 aaacggaaag atcttacccg gacgtttaca gtatatctat aatcgatttg ctatttacca
    63841 aaatcctttc tcagacgaat taaataaagg gcatcaacta gtcaacggct actatgccat
    63901 gttcaacgga ggactatttg gcaggggatt aggtaacagt atccagaaaa aaggattttt
    63961 acaagaagca caaaccgatt ttatttatgc aatcgtagtg gaagaactgg gagtgattat
    64021 ggggattttg atattagcgt tgctattctt tatgattgtg cgcattgtcc ttgtaggtat
    64081 ccgttcaaaa gatccgttca attcgctttt gtgcattggg atcggcgcaa tgtttttgat
    64141 ccaagtattt gtcaatcttg gaggaattac tggcgttatt cctttgacag ggatcacgtt
    64201 cccattttta agtcaaggag gctccagttt gctgatgctt tcgatttgtg tcggctttgt
    64261 cctcaacatc agtgcggatg aaaaacgcaa gagcttaggt ttataataaa aaaagatgta
    64321 ggagtggttc gatgaaaaaa gttttagttg ctaacagggg tgaaatcgct gtacgtgtat
    64381 ttcgtgcttg tacagaatta ggaattaaaa ctgttggaat ctatgcagaa gaagatgaat
    64441 attccgtaca ccgtttcaaa gcggatgaag catatttagt aggacaagga aaaaaaccga
    64501 ttgatgcgta tctagatatt gaagggatca tttctatcgc gaaagaatgc ggagctgacg
    64561 ctattcatcc gggttacggt cttttgtcgg aaaacttgaa ttttgcgcaa cggtgcgaag
    64621 aagaaggcat catttttgtt ggacccaaac tgcatcattt agatattttt ggagataaaa
    64681 tcaaagcaaa agctgcagct atcgaagcgg ggattgcttc gattccaggc acagatggac
    64741 caatcgcttc tatagacgat gccttggagt ttgcgaaaca atacggttat ccgattatga
    64801 tcaaggcagc tctaggcggc ggcggacgcg gcatgcgtgt agctcacgat gaaaagagtg
    64861 caagagaggg ctacgaacgt gcgaaaagcg aagcaaaagc agcctttggt agtgatgaag
    64921 tgtatgttga aaaatacatc gctaatccta agcatatcga agtacagatt ctcggggata
    64981 cgcatggaaa cgtcatccat ctttttgaaa gagattgttc tgttcagcgc cgtcaccaaa
    65041 aagtagtaga agtagcgcct tgtgtctcta tgaatgaaca gcaacgtcaa aaaatctgcc
    65101 aggcagctgt ccaattgatg aaatatgtcg ggtatgtcaa cgcaggtaca gtagaatttc
    65161 ttgttgaagg agatgatttt tactttatcg aagtcaaccc tcgcgtccaa gtggaacata
    65221 cgatcacaga aatgattaca gatattgata tcgtaacaac gcaactgttg attgctcaag
    65281 gtttggatct gcataaagaa atcggattgc ctcagcaaga aggaattaaa ttaaatggtt
    65341 cagccattca atgccgaatc actacagaag acccgttgaa taacttttta ccagacacag
    65401 ggaaaatcga tacgtatcgt tcacctggtg gttttggcgt acgtctggat gtcggaaatg
    65461 cttacgcggg ctatgtagtg acaccgtatt ttgattcatt gctagtaaaa gtatgtacgc
    65521 atggtgctac ctttgaaaca gccattcaga aaatggaacg ttgcttgaaa gaatttagaa
    65581 tccgtggagt gaaaacaaac attccattta tgctcaatgt catcacacat cctgaattcc
    65641 aatcaggaaa tgcaaaaaca accttcattg atagtacagc aacattattt gaatttcctc
    65701 gactacgaga ccgcggaaac aaaacgatga aatatatcgg tgaaattact gtgaatggtt
    65761 ttccaggtat cgaaagtggc gaaaaaccat tttatgaaga accacgtatg cctaaagatc
    65821 taatcacaag aactgattac gtaacggcta agaatgtatt agatgcgaag ggtgccgatg
    65881 ctttggtcga atggatcaaa ggacaggaaa atttgttgtt gacggatacg acatttagag
    65941 acgcccatca aagtctgcta gcaactcgtg ttcggacaaa ggatttcaag cagattgcac
    66001 gtttgactgg agaaggtctt cctgaactct tttcaagtga gatgtggggg ggagcaacat
    66061 ttgatgtggc ttaccgcttt ttgaatgaag atccttggca acggttgaga aaaattcgtt
    66121 ctttaatgcc taatacgttg ctgcaaatgc ttttcagagg ttcaaatgcc gttgggtatt
    66181 ctaattatcc tgacaatgta ttggtggaat ttgtcaaaga agcagcagcg caaggaatcg
    66241 atgtatttcg aatctttgat agtttgaact ggactccaca aatggaaaaa agtatccaag
    66301 ctgtacggga tactggtaaa atcgcagagg cagctatttg ctatactggc gatatcaacg
    66361 atccaagtcg ggcaaaatac aatgttcagt attataaaga catggcaaag gaactagaac
    66421 aactgggtgc gcatatcatt gcaatcaaag atatggctgg gctgcttaaa ccgcaggcag
    66481 cttatcgttt gattagtgaa ttgaaagaaa cgacagatct gccaatccat ctgcatactc
    66541 atgataccag cgggaatgga atcatcactt attctgctgc ttcaaaagca ggagtagaca
    66601 tcgttgacgt ggcaatgagt gcaatgagtg ggaatactag tcagcctagc atgagcagct
    66661 tgtattatgc actggtcaat ggtccacggc tgccagaaat cacgattgaa aatgcacaga
    66721 aattaaacca ttattgggaa gatgtgcgca tgtattacaa accgtttgaa aacggcttga
    66781 atgctcctga aacagaagta tatatgcatg aaatgccagg aggacagtat tcgaatctac
    66841 agcaacaggc aaaagctgtc ggattaggtc atcgctggga tgaaatcaaa caaatgtatc
    66901 atactgtcaa cttgatgttt ggcgatatcg ttaaagtaac tccatcatct aaagtagttg
    66961 gagatatggc actatttatg gtccaaaatg atcttacaga agaagatatt tatgaaaaag
    67021 gcgaaacact aagtttccct gaatctgttg tgactttctt ccaaggagaa ctagggcaac
    67081 ccgttggcgg atttcctaaa aaattgcaaa agattattct taaaggcaga cctgcattga
    67141 atgaacgtcc aggtcttttg gctgaatcag tcgacttcaa cgaagtaaaa aaagaattgg
    67201 cagaaaaaat tggctatgaa ccaaaacaag acgaagtatt aagctatttg atgtatccgc
    67261 aagtcttttt ggactatcaa aaagcatata atcaatttgg cgatgttaca ttactggaca
    67321 caccaacatt tttccaaggt atacgtttgg gtgaaacaat caatgtacaa atcgaacgcg
    67381 gaaaaacgct tatcattcgt ttagatgaaa tcggagaacc cgatatcgaa gggaatcgcg
    67441 tactgttctt caatttgaat ggacaaagac gtgaaatcac tatcaaagat aattcgatca
    67501 tcagtacggt ccaaacccga ttgaaagctg aaccgacaaa ccgagaacaa attggagcaa
    67561 cgatgtctgg ttctgttttg gatgtattag tgaaaaaagg cgataacgtt aaaaaaggtg
    67621 atacgttgat gattactgaa gcgatgaaaa tggaaacggc catcgaagca cgttttgacg
    67681 gagaagtcgc acatgtatat gtgtcttcag gagacactat ttcgtctggt gaccttttga
    67741 ttgaagtgac tgagaaataa taagcgaaag gtggggaaac atgaaaagac tgggtttgtt
    67801 ttggctgatt ctgctattag ttattgtggc attctacatg gaaccagtct tgtttccgcc
    67861 aaaacttgaa gtgaaggaaa actctgatca acaaatatca aaccaaatga caacgtggaa
    67921 ataccaggag cttaaagcaa ctggtttttc tacttatatc ggaaaaccga tcgatcagct
    67981 ggaaaaggat tttggccaag cctatgatcg attaactagc ggatttggtt ttgaaacaag
    68041 atattatgaa aataaagcta gtcatctcac atttgaagcg aacatccaag acagcagagt
    68101 cacaactgtt aaagtactgc aaacagggcc tgctgatatc gcgccgtttt atttagggat
    68161 gacgatgcaa gatcttacga aaatcactac tatctatact aatttcactt ttgagtacca
    68221 tgatacagaa gttggaatcg aactaatgga agaggatatg aattatcgtc cgctaattgc
    68281 ttttgataac gacacttttg ctattttatt ttttgatcaa acaacaaacg gcctattttc
    68341 agttgtgtat ttagataaag atagtctttt gaaactattg ccatatcaag tgtttggtga
    68401 aggccttcca cattatcaag cagataaatc ggctgattgg gaatcaatca atcgcgttaa
    68461 agaacgaaaa agtacgactt tattaaatat gcttagaaga gaagacgaac ttcccatata
    68521 caatcaaaca cttgcctttc aaaaccacag tcagctgcta ctacatgatt ttttgacaag
    68581 gcctgaggaa atattgtcag aggaacgtat ggctgattgg caaaaatcgc taggcagtgc
    68641 tcaaacgaca gtgaaattct cactgacgaa tgatgaattg gagaagttaa tcagtaaaca
    68701 aaaattagaa aatactagtg ggctatttat tcatccggcg attgacgcta ctttttcgtt
    68761 tttatattgg tacagcgatc cttacaacca tgaccgtttc atggacccgg gaacggattc
    68821 tgtaggcatt gctttttcta aagaaaatat gttagtctta ttgcaagaag aaaacggaga
    68881 aagtagtgat agcagtgatc gttaacgaag agctgtttca actggaggat caatgcctcc
    68941 aagtcgctga aatgataaaa aagagtaaaa cgatggcttc ttatcttgaa cataaacagt
    69001 caatggaaga atctgcaaaa gtttgccagt tgaaagaagc gttcttgtct aagaaagaag
    69061 catttgaaaa tgtagcggca tatggtgagt atgcacctga ttatcgtgaa aaaaaacgag
    69121 ctgttcgaaa agcaaaaaga cagcttgata tggatgagca tgttgcagct ttccgatata
    69181 gtgaaacaca gctgcaaagt ctgttggatc aaattgtcca agtacttgcc gaagaagttt
    69241 ctaatgaaat caaagtagat gcaggtagcc cgttttttga aacgaaaaaa cactttggat
    69301 gcggaggtaa ttgtcatgca attagacaat agtgaatttg aaataacaaa acgacgcggg
    69361 ctgatcgtat gggtctacag cttaaaacaa ttaaagaatc tgaaaaagtt cggcttgttg
    69421 cattatgtct caagaagaat gaaatatgtc gtgctttact taaatgaaga gacattggaa
    69481 gaaacagaag aacgaatccg taaattacat tttgttcgaa acgtagaacg ttcttaccga
    69541 ccggatatcg aaatgaattt tgccgaaaaa ataggaaaca aaacagtatt aaaagacgaa
    69601 ggtttcgaaa ttgaagagct aaatacagaa atccgattag ctgaacatct ttaaaaatct
    69661 cttatgagca gtttttaaat aaaaaataac ttggataacg atcgattttt gatcattgtc
    69721 caagtatttt tttggtatac tagtaccagt accatttcta aggagtgagt agatgcggat
    69781 aatatcagga aattatggcg ggcgccgttt aaaaagttta gcaggggcga acaccagacc
    69841 aactacagat aaagtaaaag aatccatatt caatatgatt ggaccatatt ttgatggaga
    69901 gacagtattg gatctgtttg caggaagcgg tggtttagct atcgaagcag tatctagagg
    69961 ctgcagtcat gcggtatgcg tagacaaaaa ttaccaagcg ctaaaaatca tcaaagaaaa
    70021 catcgaaatt acgaaagaac cagagaaatt taccacaata aaacttgatg cggacaaagc
    70081 aatcgaacgg ctagcagcgg agaaacaaca attcgactac ttgtttcttg atccgccata
    70141 tgcaaaacaa aaaataatcg atcaaatcca taaaatggaa gaactgggac ttttttctaa
    70201 aaccgcagtt atcgtatgcg aaacggataa aacagtcgaa ttgccagaaa ctgtggctgg
    70261 attccatcaa ataagaaaac agatttatgg cataacggcc gtaactatct atagaaaaga
    70321 gggaaatcaa tgaaacgtgc attattccca ggaagttttg atccttttac aaaaggtcac
    70381 ttagatacgg tggaacgtgc ggctaaatta ttcgatgagg tagtaattgg tgtttttatc
    70441 aatacttcaa agaaaagttt gtttccccca gaagaaagaa tgacattgat cacaaaagct
    70501 gtttctcatc ttcctaatgt aaaagtcatg catcaagaaa atcaattgac ggtagaaaca
    70561 gcaaaagaga ttggtgcaga tgcgttgatc agaggcatac gaagtatcaa agactttgaa
    70621 tatgaacgag agattgctca gatgaatcat catctatatg atgaattgga aactgtcttc
    70681 ttattagcaa aaccagaata tagtcacgtg agttccagta ttttaaaaga agtactgcac
    70741 tttggcggag atgtgagcag ctacttaccg ccagtgatca acgaagcttt agctggaaag
    70801 cgtgaatcaa atgaaagcta aacaatggtt gaaaacagta ctgattatca tatttggaat
    70861 gttgatcatt ggatttgtag tagttccctt gccgtattat gtagaatcac cgggagccac
    70921 gatcgatctg aaagaactaa ttacggtcaa tgataaggaa gataaaggtt ctgggtcttt
    70981 ctctcttacc tctgttggta tcaggcaagc tacaggtttc ttagctttaa aagctaaaat
    71041 gacccctttt gaagaattga tcagtgaaga agaattgact ggtggggcga caaatgaaga
    71101 atataatcaa atgcaacagt attatatgga ttcttcccaa aatgcagcaa tcgaacaagc
    71161 gttaaaactt gcagacaagc cttataaaat ggaattcaaa ggcgtatatg tcatgagtgt
    71221 agagaaaaca tcgaatttct ttggcaagct ttcagttgga gatacagtaa caaaagtaga
    71281 cgggaaaagc tttcaaagta cagaagcatt tatggattat gtaaaaagcc aaaaagttgg
    71341 acaaacaatc gaaatcagtt atttgcataa tggtgaagaa aaaacagctt ctggtgatct
    71401 gatcgagtta ccaacagata aaaaggcagg aataggtatc acgcttactg accataccga
    71461 gatcgaatca gatacagatg ttcgtattaa ttctggctct atcggtgggc catcagcggg
    71521 attgatgttc actttagaaa tctatgaaca agtcactggt aaaaacttgc gacatggtaa
    71581 gcaaattgca ggaactggaa cgatcaacag tgaaggagaa gttggccgaa tcggaggaat
    71641 cgataaaaaa gtggcaagtg cggataaagc tggcgtagag attttctttg caccagatga
    71701 tacgatttca gacgatgtta agaaagaata tcctgaaata aaaaccaact acgaagaagc
    71761 aaaagctgcc gctaaaaaat tgaattcctc aatgaaaatc gtaccagtaa aaacagtaca
    71821 agaagcgctt gactatctag agaaaatgaa atgaaactaa ataattaaag aggggttgtg
    71881 acacgagttc tgtcacaacc cctctttttc gtcaaaaatc ttcacgataa aaataaaaac
    71941 aaaagaaaaa attgacgtaa ttagatgttg aagggacgat tttgccagag ctcactgttt
    72001 cttcaatttt aaatgaaagg agtaatgaat gatttttact ggtcattctt tgaaaaaaga
    72061 acaatgatag aaaaaatcag caagaaaata ctagcttcac ccaaattgat catttggatg
    72121 tctatgatag ttcttatctt ttgtatcgca agtggttttt tattatcaaa tttgctattt
    72181 tatagagaaa cagaagagac tgctgcaaaa gaaatacttt cttcgtttag ttccagtgga
    72241 gaagatccat cagctgaagg aaacgatgta gaagaggatg ctcgtccaaa ggtcatgtac
    72301 accgatatca aaggaagtgt gaaggagcct ggtatctatt ccttttcttc agaagaacgg
    72361 gtctatgatg tactaaagcg tgcaggtgga ttattagaag aagcggatag cgatcgaatc
    72421 aacttttctg cgaagataga agatcagcaa gtcctatata tccctgctgt aggtgaagag
    72481 ctgccagaac atcttgatca gttggcatcc tcggaaggaa aacagcctac agcagattcc
    72541 gagcctccca aaataaatat caatacagcc actccatcag aacttcagca gattcctggg
    72601 atcggatcag tcaaagcgca agaaatcatt cgatttagag aagaaaacgg atccttccag
    72661 aaagtggaag acctgcagga gatttctggt atcggtgaaa agacagttga aaaactaaaa
    72721 aacttcgtta caattaaata gaatgattca aaatcgactg gcacgagcaa taaatggacg
    72781 ctaatggata ctaagaataa tccaattcta agagcttggc aggacgagga ggtagtattt
    72841 ttggatagga aatagcttga ttgttcgcaa agaaacatta aaaaaaccaa tgctgaatgt
    72901 ctatctccaa aataaaatat ctgggattca tattatgaat acggctgtca gcggcaataa
    72961 cagtcaagct ctacgagaac gctttgccaa agatgtactt agctatacag cagataaagt
    73021 atttattctc attgggacga atgatctagc agagcataaa caactttcta aagaaacgta
    73081 ccaaaaaatc tgcagtggat gatagttcag ctgaaaaaag actatagttc tgatcatgct
    73141 ttctttatca cgccaccagc agtcgatgag acaaaacaaa aatatcggaa taacaagttg
    73201 atcaaagaat atatctctgt tctcacagaa gtagcaaaac gccatgattg tgaagtatcg
    73261 atttttatac ggaatttctt gaacaaatgg attcgaagaa tttttataat gggctaatgg
    73321 atgacggact tcatttcggc agtgaagggt aggatctttt gtcagacagg atgattcgtt
    73381 atatttaaag aatattttgt ttaagcaatg agtacttttt aatcaacttg aaaaaacgga
    73441 ggaatataaa acatgttaga aaaatcttca aaagcatgcg atacgaataa agaaaaagtt
    73501 actgataagt cacataatga acgcattcct tgggaccagt actttatggc tcaggcagta
    73561 ctgttatctt tacgtagtac gtgtacacgt ttagaagtag gagcgacact cgttcgagaa
    73621 aaaaggatca ttgctggcgg ctataatgga gcagtcagtg gagatgtcca ctgcatagat
    73681 gaagggtgtt acgtcgtgga tggacactgc ttgcgaacga tccatgcaga gatgaatgca
    73741 ctcctacagt gtgcaaaact tggtatcccg acggaaggtt cagaaatcta tgtcacacat
    73801 tttccatgtt tggcctgtac aaaggctttg ttgcaagctg gtgtgaaagg gatcaattat
    73861 ctccatgact atcgaaatga tccttatgcc caagcgttga tcgaacaatt aggtgtatct
    73921 gtacaccaag ttgctttaga ctcacagcat ttttcaaaac ttcaaaatga attaacagga
    73981 aaacatgtct agcgttttag aaaggcatgt tcgaaatcgg ttgatttttc cggcaattac
    74041 gtcgatgatt atctgtttgt tgtattacac tgacaattgg ggttatcgtt tgcttcttgc
    74101 cggaattttt ttatggtttt cgctaaaaga aggacgtttt atcgcgtact tgtgtatttt
    74161 gtgtagcata acggtatcca tttcttatag gctacaagag tattcatttt tagaggaaaa
    74221 aacaacatct gcgcaaatta ctgtatttac agatacgatt cgtgcaaatg gtaatttttt
    74281 gacaatggaa ggaaggttat cagagaataa acaaaagctc cagctttctt atcaagtaaa
    74341 cacagaagca gaattgaaaa catggcttaa cttccgagga aagggaatag aattgactgc
    74401 tgaaggcgag ttcatctctc cagcaaaaaa gcggaatctt tataattttg accaagaagc
    74461 ttacttcagg aaccatagaa tttctggacg gttccaaatt caaaagattg aacagatcac
    74521 catgcaaaat cactggcgaa actggcttca gagaaaaaga ggatctttta tctcctttgt
    74581 ccaatctgaa tttccgcaaa aaacaagtgt atatattaat tctttattat ggggctataa
    74641 agatgcagga ttcagagaac cagaagaaat ttttagggag agtggtttgc tgcatctgtt
    74701 cagtctatcg ggtctacaca tccaatttta tttagggtgg ctgtattatt tatttcgacg
    74761 tatcggatgg ccactacacg tcagtattat tccgttgagt ctactaacta tctgttatgt
    74821 ttctattgca ggaagttcta tttctgtttt gcgtgctagt ttgctgtttt tattgcgatt
    74881 gctagtcaaa ttgctgaata agcattattc caccatggac ttatttgcag ttgtcttttg
    74941 gcttctttta ctatttgatc ctcgatttct ttttcatgcg ggtggacagc ttagtttttt
    75001 catgagtttt ttgtttatcc tgatttcttt tgatggagat tggttgaaaa aaacggcctc
    75061 tcacgtcctg tttactatga tcacgatgcc gttgattgta tggcattttt atgagtggcc
    75121 tattttaggt agtcttttta cattactgat tgcaccggta ttcaagtttc tttttcttcc
    75181 tggtgtgtta tttctcacac tcttcaatcg ctggctgccg gaaactttac ttttgttgat
    75241 agaagaggga ttaatcttat ttgagcgaat aatcgatttt tcttcagggt cgtctctcat
    75301 tataggacgt ttgccccttg tgctatctgg gatgtatatt ttcggattac tgatttgtat
    75361 ggataggttg aaagaacaac ctgtgaaaca cttgttttat gttggtcttt tttcttttat
    75421 ttttttgact ttgccatttt tacgcttttc ttcaacgatc gcttttatcg atgtcggtca
    75481 aggagacagt atctttttgc aaagtccttt tcgaaaagaa acgattttga tcgatactgg
    75541 aggtcagtta cactttgaaa gagaaaagtg ggcgcaaggt atgatacgtc ctcctgctga
    75601 aagaaatatc gttccatatc taaaaggtca ggggataagt gtaatcgata aactcatctt
    75661 gacacacgac gatacggatc atgtgggaga attacccacg ttatctcagc attttactat
    75721 caaaaaaatt tatatcggtt ggggcgctgg acgaaatgag ccattacaaa ggcatttatc
    75781 tgaagctcaa aaaaatggta ctgagatcat tgaaatcaaa catggggaca ggatcagcgg
    75841 ttattatgat ctttatgtat tgactccttt tgaaaaggga gagggaagaa atgaagattc
    75901 gattggtcta tggatggaat ataacaatag gcgcttttta tttttaggag atttgaatca
    75961 agcaatggaa aaacaattgc ttttgattta tcctaatctg aaagccgatg tagtcaaatt
    76021 aggtcatcac ggtagccgta cgtcaagtaa cacagatttt ctttcccata ttgaagcaaa
    76081 acatgggatt atatcctgcg gcgtaaataa tcgttatggt cacccgcaca gcgaagtatt
    76141 ggagacacta gaagaaaatc agataaaaac atggcgaacg gatcaacaag gaatgatcac
    76201 ctatcgctgg catccagttt ttcatcctca tgggctagtg gaaacaatga tagactagta
    76261 attctcatct gctcatggta ggataatttt tgaaagtggt gagtaatttg agtatacaag
    76321 cagagttaca aaaaatacgt aaagaaaaac ttgcatcttg ttacttgatt ttaggaacag
    76381 aaaagtttct ccaagatcaa gttcggacag aaattgtcaa aaaaatccaa ttagagggcg
    76441 aagatgactt aaatttttta agttttgaca tggaaaatgc aagtttagat gaggtagtag
    76501 ctgaagcaga gacgttgccg tttttcggag atcaacggtt agtattcgta gagaatcctt
    76561 attttttaac aggtgaaaaa gtaaacaatg gaatcgaaca aaatactgat ttattgacgg
    76621 cttatttgaa agaaccatta gaatcgactg tattagtatt ctttgcgcca tatgaaaagt
    76681 tggatgaacg gaaaaaagta acgaaacaat tgaaaaaaac agcaattatc atcaatgtcc
    76741 aacagttgaa tgaaaaagaa gtacgtcaat atctgatgaa taccttagag aattcctcaa
    76801 tcaaaatgga acgaagtgca attgatttat tcttgaggtt gaccgactta gatttatcca
    76861 aaatgatgag agagttacag aaattggtcc tttatggaca aaatcaacag caaatcacgg
    76921 taagagaagt agaagaatta gttcctaaaa cgttagaaca taatattttt gacatgaccc
    76981 aatatatttt aaaagggaag acggaacaag ctttgcgttt atacgaagat ttagttatgc
    77041 aaggggaaga aacaatcaaa attaatgcga ttttgctttc tcaattacgt ttatttttac
    77101 agacaaaatt tcttgtaaag attggttatc agcaggcaaa tattgctgaa acattgaaaa
    77161 tccatccata tcgtgtaaaa cttgccatgc aggaagtaaa aaaattcgat gaacatttgc
    77221 tggtacagtt gtttgatcaa ttagttgaga tggattatca aatcaaaacg ggacaaatcg
    77281 aaaaagaatt aagttttcaa ctattcgttt tgcgcacagg gcagttgtta agaaatcgat
    77341 aagaagaaag acgagggcaa agcatgcggg aaggtcgttt gtttttgtcc tttttttgac
    77401 aaataaaaaa atcggccgaa gccgattctt ttatttagcg atttttttgc ttaaacgaga
    77461 tttgtcgcgg ttagctttgt ttttgtggat tagacctttt gattcagcca tatcaatagc
    77521 tttaacagct tctttatata acgcatctac gttgtcagca cctgaagcta cagcatcttc
    77581 aaattttttg attgcagtac gcatagcgct tgtttgagat gaatttttca cattagcgtt
    77641 ttcgcttgta cgaacacgtt tgattgcaga ttcaatgttt ggcattagtt tcacctccga
    77701 taacttaagt tcttacgcgt gttaccgcat aattcaacat tcattattat acctaaaggc
    77761 agcgtgcttt gcaatatgaa catgacaagt ttttttcctt aacagtcttt tattaggatt
    77821 ttttttcagt aaaaacggat agtacaagct gaaaaaaatc aaaaagacgc tattgtggga
    77881 ggcaatagcg tcaaaccaaa ctagattttt atttatctat ttaaacaaag tttgaatggg
    77941 aatgtttggt actaagttat tatataacaa agagaaccgt ttgcaaagtt ttttttgaaa
    78001 agggatacga ttccctgttg ttctttgttt ttctattaag aaactgcagt tattttataa
    78061 ccagtatcct ttgatcagta aaggctattc tggtgtaccg attattttaa tgtattcatc
    78121 gatatttttt atcaacattt tttttatgac caactggttc cgacggttcc atttgccact
    78181 tacttccagc acatatttat ttaggggcaa acgtaagata tattttgcca aatagcgatc
    78241 tttaagcagg ccattgatca cacggtcctc aagctgcaag ttgatcccag tcagcatcat
    78301 tggctgttct tgtaaaacgt acgtatcaat taaaagacct tcaaaagttt ccacaagcgt
    78361 cttcctttct tttcaaagta acctgaaaac aggcactaat tcttccattt tgatattcgt
    78421 tgaagtggat gcgatcattc tttcttttgt ccactgtaat ctagtataac aaaaaagagc
    78481 agaagagcca aaaagaaaaa agagcatcga attctatagg aagtctcatt ggcacggcct
    78541 aaattattac aaaacattga ttaaaaaaag cccctgcatc gcaggagcat cccctatatc
    78601 gttttaatag gtcaatcaaa aagcagtatc aaacacattt tgtatcaaat caggaaataa
    78661 tagataagct aagcccagaa tcaaaagtac cgctagaaga aaccagccga ttgttcctat
    78721 gaaatacttt aatttttcga acattgtatc acctcgttgt cagtatatca cggatcagta
    78781 aattgggggt gttaagaatc tcctaaaata gacaaaattt gttctatttt cctaaagcaa
    78841 atacttgtaa gatacagtaa gtgataccag aagcaatgac cggtccggcg gcgattcctc
    78901 ttaagaagac aacacctaaa atcgtgccga aaacaagtgc aacagtgatt tgtggatcag
    78961 aagcaatcaa tcctacaccc ttggaagata agacagccac tagcccaccg caaaaaacag
    79021 ctacccagcc aataggggat ttaaaggctt ctaatagatc acgtagaccg atttctcctg
    79081 ttgcgatagg gattagaata gccgctgtga tgatcgtcac cccaatattg attcctttgc
    79141 cttgaagcca agggaaaaag gaacgggagg ctggtatgag cttcataatc aaaacaaaag
    79201 ctgccgctat catcagactt tgatttttag ctaaaattgc tacaagtaaa ataattccta
    79261 aaaaaagcca actttccatg aaatatattc ctcactttct tcgaaacaat cataacatgt
    79321 ttttagaaag ttacaaaagt aggaattcat ccttcaagac ctattcataa gctgtttttt
    79381 tcataaaata aacaaaatca taaaaaaaca ctttgcttat ttgacagaag acagccgcgc
    79441 tttgtatagt ggacaagact tacatttttt tgaagaatca gaaagatcag gtaaaggtct
    79501 tagtaaacgc aagccattca gaataaccaa tattgtgctt ccttcgtgtc ctacaactcc
    79561 aagaggtaga tttaaaaatt gcaggaagtt agaagcaatc aacaagagaa taacaagaat
    79621 cgaaaaaata atattttgtt tgatgatccg ccgcattttt aaagatagcc gatgactgta
    79681 gacaaattta tttaagtcgc ttttcattaa gacgatgtct gcgatatcca tagcggcatc
    79741 tgtcccttgc cccatagcga cacctaaaga agcggctgct aaagcaggcg catcattgac
    79801 tccatcacca accatcgcat ttacttggta aagttcattc tcttttttga ctagttgtgt
    79861 tttttcttcc ggagtacagc ttgcatagta atgttcaatc cctacttgtt cagcaatggc
    79921 ctttgctgtg ccagaatggt cgccggttat catacttgta tggatgtttt gggatttaaa
    79981 gtaggaaatt acttgttggg tatttgcttt tggaatatct agcagaccta aaacagcaat
    80041 taactgctga tctttagaaa gataaatcac ggttttgcct tgattttcca aacgttcgat
    80101 catttccgca atctctttgc tgattatcat cgtctcagga tcatatgcgt gcttgccaac
    80161 tttccattgc gcacctaaat aggtagtttg taatccaaaa ccagtaattt cttctattgg
    80221 taagtattgg atttcttcag gtattgtgca atcgaaatat tgcaaaatcg catgagcaag
    80281 tggatgcgtc gttttttgtt ccatagcaac aagaagttgt tgtgccaaat ccttttcatc
    80341 aaagaaaaag ctgtcagtta caactggttt tcctttagtg agcgttcctg ttttatcaaa
    80401 ggcaatcgct tttaattcag aaagcttttc taaatggata ccgcctttga ttaaaatccc
    80461 attttttgca gaatttgaaa gagcagctag agaagctggt gtagcagatg cgacaagcgc
    80521 acaaggagaa gccacaacta ataataccat tcctcgataa aagctttcat cccaactcca
    80581 acctaggaaa aagtggggga gaaggatcat tagaggaaca gcaagcaaaa tcaattttac
    80641 ataattattt tcgattcgtt cgataaatga agctgtttga gttggcgtat tctgcgcttc
    80701 ttctactaat tggataatct tagcaaagac agtttcggaa ctcgtttttg ttactcgaat
    80761 cgtgatggga tttccttgat tcagcgttcc tgcatacatc tcatcgccta aatatttttc
    80821 tacaggcaca gattcaccgc ttaatgcaga ctcatcgatc gttgcctgtg tgctttctaa
    80881 aaagccatct atcggaacag ctgctccttt agcgacaaat accaagttat ccacctttag
    80941 ttgagaaaca tctacttctt gcatttgtcc attttctttt aataattggg ctttttgtgg
    81001 ttgaagattc atcaaagcag taatttcttt ttgactttta ttagttgtgt attcctctaa
    81061 tgcaccgctt aaacaaaaga tgaatgtcaa cattgctcct tcaaagtaat taccgatcaa
    81121 acaggcacca atagcagcta aagccatcaa taaatccaca ttcagtgtcc gatcattcca
    81181 caattctttc aagccttccg atgtttgttt atatccaccg atgataatgg cactgatata
    81241 aaaaagtggg gtgatggtaa gagagaatcg gtcgaagata aaaccgaaga tcataaataa
    81301 aaggcagccg attgttgctg caaaagcttt tttctgatta gtcatcgtta ttagacctcc
    81361 ttgtcaaaac aaggatagca taataaaaat gtaattgata ataataacag ctaaatgaga
    81421 acgataatgg ttatcatttg gtgattaagc ttgatgttgt ttttattatt tataatgatt
    81481 attattaaag aaaataaaaa accagaacat ttctcaaata agagaagtgt tctggttttt
    81541 tctacattgc aataaaccaa taaccaatca aaatgatacc aaggataata cgataccagc
    81601 caaaagcggt aaagtcgttt ttcttcaaat agcttagtaa gaatttgata gctaagacgg
    81661 aaaccacaaa agatacgaat gttcctacaa gaagaacaac agtttcttcc attttgaatg
    81721 aattcccttc agctaaatat ttgacgatct tcaagccgct tgcgccaaac atgacaggaa
    81781 tccctaagaa aaatgaaaac tctgtagcga caaaacgcga agcaccaatc aagatagatc
    81841 ctaggatcgt tgcgccagaa cgggatgttc cgggaacaag tgccaagact tggaataacc
    81901 caacaagaag agctgcttgg tacgtaaagt cattcagaga agtccaacga ggtgttttgt
    81961 ttttgttgcg tttttcgaca ataataaagg caataccgta aacgatcaaa acaatggcaa
    82021 ctggtaagaa cttgtggaaa tttgcttcta accaatcatc caaaggcaaa ccaatgatga
    82081 tggctggtat gcaagcaacg atcactttaa accaaagtgt ccacgtatct tttttttcta
    82141 ccgttgattt acttggagaa aatgggttta acttgtgaaa ataaagaacg acgacagcca
    82201 agatcgcccc taattggata acgacattga acatcgacat aaattgtgaa ctggcatcca
    82261 gtttgatgaa ttcatctgct aggatcaaat gaccagtact actgatgggc agccattccg
    82321 ttattccttc aataataccg agaatcactg ctttgataat atttgcaaaa aacataaatt
    82381 ccttccttcc caattaagat acatcttata gtataccgtt attttttgag aaacccaatc
    82441 tgttttttaa tattaagaaa aacttgagaa ataaagcaga taagtacacg aagagaaaaa
    82501 agaagacaaa gccctcgttc agactttgcc ttttatgatt ctctggtaaa aattaaaata
    82561 tccccattat agaaacgagt cgcgactatg tgttttcccg ttatcgatat aagacgtatc
    82621 attgatgccg tggatagaaa atcgaccgtt tgcataagtg acctttgtta cgctgccatt
    82681 taaaagccca gcacgaacag gctgtttagg atcgatcaga ttaagcaaaa aggcgatcgt
    82741 caaaccatga gatacgacta aaacatttcc accaccattt ttttcgcagt gatgggcgat
    82801 atcttcaaat cctgtccata cacggtctcg caatgtttca taagtttccg cccacccagc
    82861 ggtatctaag ttgtatacag cattagcaat ttgttcaaaa gaaacttctg tggtaaacat
    82921 ttcatcatat gttttaaaat tcaatacacg agggatcaca ccccacattt ccatgtcata
    82981 tccaccttca agagacccga aacaccattc tctaatcctt ttttcaataa aataaggaat
    83041 ttcttttccc ttagaatgtt ctcctagaat aatacgggca gtttcgattg cacgaccact
    83101 gtcactagaa taagcaaatg aaaagtcgac attttttaac ccgcgaccta aataatgaat
    83161 gccttcttgt ccttgctttg tgaggggtgt atcacaccag ccttgtactc gttcaattgt
    83221 attgaacatc ggttttccgt gtcgtacgat ataaagtgta gtttctgtca ttttattctc
    83281 ctcctaagtc gttttaacga ttgaaaaaag gatttgtcct tttttcctgt tcaatcgtcg
    83341 ttgcatcgcc atgtcctgga tagactgcaa attcatcagg taatacaaac agctgggtcc
    83401 gaatactatg aagtaattgt tctaaattcc ctgtatacag atcggttcta ccgatacttc
    83461 ctctaaacag ggcgtcgcca gaaacgacaa aatcatcaaa aataaagctg acgcttccta
    83521 tagaatgccc tggggtaggg acgacgcgaa aagacatgtt tcctaaagta taagttttca
    83581 tttcaaattc atattctgca ggcgagacaa taacatcagc aatatcatca tgtctaccga
    83641 gtccagaaag attcaaaatc ggatcaccta gccatttttg ctctagtggg ctgacataaa
    83701 ctgggatttg ataatacgcg cgtaaaggtt ccactgcacc gatatgatcg tagtgagtat
    83761 gggtaagcaa aatcgctacc ggctgttggt ttgttttttt gatttcttgt ttgattcgtt
    83821 ccgcatcttc tccagggtca atgatcaaaa gtgcttcttc gttgtagacc aaataacagt
    83881 tttcttcaat cattcctgtt tttaatcttt cgatatgcat gtgcattcct ccttgatagc
    83941 caaaacttct ttcatccatt agtataacgc attcattgct aggaaacaaa aacaggtact
    84001 tatttctctt tacttatctg aattatcaga ctatatgaaa agtatttgga tattctaaag
    84061 agaaggaaag taagttttca tagcatttta atatgtcctt gttacattag attcatccaa
    84121 aaatccaaat tctatatcct atatccctgc ctaatcagca gggtgttttt ttatgtttgg
    84181 aaagagagct ctttttgcta aactaggttt tttcaggtaa tcttagagag aaagaggtgg
    84241 tggaaatgat caaacagtca aaaatatctg tgcggcactt agtagaattt attttgcgac
    84301 gtggaagtat cgacaatcgt aaaaaaagta accatactgc attagaagga gcgagaatcc
    84361 accgcaagct ccaaaaagat gcgggagaaa actatgaaaa agaagtcttt ttaaaaacaa
    84421 cagttcaatt agatgaatat tacctgatcg ttgaaggccg tgcagacggt attttcaaaa
    84481 gagacggtat ttattatata gatgaaatca aaacctccga accacgattt gaagatttag
    84541 aaccagaaca agtggaactt ttctttcatc aagcaagggt gtatgcctat atctatagtc
    84601 aagaacatga tttacaagaa ataaatctgc aattgactta cttccagacg gcagaagaag
    84661 tgatcacgag aaaggttgaa catcagacaa gagagcagtt agaaactttt tttaaaaagc
    84721 tgactgaaga ttataaagaa tggcttgttt ttcaagaaaa ctggcgcact gtacgcaatg
    84781 cctctttgat ggctttgaaa ttcccccatg atgagtatag gaaaggtcag cgtgaactag
    84841 cggtagctgc ttataaaacg atccgtacga aacagaaatt gtttgtagaa gcacctaccg
    84901 gaacaggaaa aacaatctct acgttattcc ccgctttgaa agctgtaggg gaagaagagg
    84961 gagaaaagat cttttatcta acggctaaaa caataactag acaagtagct gaagatgcta
    85021 tgacagcatt aaaagataca ggagctgaag taaaaagtgt cactttgacg gcgaaagaca
    85081 agatctgctt tttagatgag acgacctgta atccagatca gtgcccatat gccaatggtt
    85141 attacaatcg gattaatgaa ggtttatggg atcttttgaa tcatgagaat cagatcactc
    85201 gtgaagtcat cgaaacttat gcaagaaagc atacactttg tcctttcgaa ctatcattag
    85261 atgtcagtat ctggtgtgac gtaatcattg gggattacaa ttatttattt gatccaactg
    85321 tttatttaag acgttttttt gaagatgaaa aaaacgagga ctatctgttt ttgattgacg
    85381 aagcacataa tttggtgaat cgttcccgag agatgtattc ggcagaacta tcttatgaaa
    85441 aaacgaaacg aagtaaggaa gctataccaa aggaattcaa aaaacttcat cgccgtttca
    85501 acaaactatt aaaagaattt gattcgatcc gagaaatagc aaaagaagat cattgggatt
    85561 atcatcatca aaaagcaccg gctgaatctt tagtaaaggc aggttatcaa ttaagcgaaa
    85621 aaattaaaga atggctggca gaatttcctg agcacccatc acaagaacaa ttgcttcctt
    85681 attattttga tttgcttcat tttttgaagg tcagtgaatt ttatgatgat cactacgaaa
    85741 caacagtcga aaaaacgtat cgcgatctga tagtaaaaga gttttgtatt gatccaagct
    85801 tgtttcttga gcagtcgttg gataaaggaa aaagcagtat tcttttttct gctagttttt
    85861 caccactctc ttattatcag gaaacactgg ggggacaaaa aagtctggct tacaaactgc
    85921 cgagtccttt cccagaagga aaccagcaag ttttcatcgc aaattaccta gagacgactt
    85981 atcgaaaaag ggaacacagt ctggcaaaat tagtagagac gattcatgct tttgctgatg
    86041 gaaaggtcgg gaattatttc gttttctttc cttcttatca atatttagat cttgcggtgg
    86101 aagcattcag acagcaatat cccgacagca atgtgctaat ccaagaaacg gacatgaatg
    86161 aggaagaacg cgaacgattt ttaacaaaat ttcaaacaaa tcccgctgaa acattgattg
    86221 gattttgcgt acttggcggg attttttcag aaggaatcga tttgagaggg gaccgattga
    86281 tcggcagtat gatcgtaggt gtaggtttgc cgcagatgaa tcatgagcag gaactgatca
    86341 aatcatatta tgatgagaag gaacaccttg gatttgctta cgcttatcag cttcctggaa
    86401 tgaacaaagt cttgcaagct ggtggtcgag tgattcgtgg catggcggat caaggaatcg
    86461 ttgtattggc agatcaacga ttttctagtt tttcttatcg acgtttattc ccacaacatt
    86521 ggcaaacagc aagagaagta cgaagtgttg gggaattgga agaaggaata cagcgttttt
    86581 ggttatcaaa agaagcagta aatcaaaaaa acgaggaatg acagacgtca gcctcgtttt
    86641 ttgattaacg atagttagtg aattgcaact cgattggtaa gtcaagttta cgtaataaag
    86701 caatcacagc ttgtagatca tcacggttct tacctgtcac tctaatctga tcttcttgga
    86761 tttgtgcttt tactttcagt ttttcatttt tgatagctgt agtgattttc ttcgcgtttt
    86821 cacgatcgat tccgttgacc aaatcggcag tttgtcgagc tttgccacct agagcgtgtt
    86881 ctgattcgga aaaatggata tttttgattg gcacatttct tttgatcagc ttaccaaata
    86941 atacatcttt gatctgttcg attttgaaat catcatcgct caatacaact agattgctgt
    87001 tctctaattt gatttctact gtagaatcct tgaagtcaaa gcgattggac aattcttttg
    87061 ttgtttgctg aatcgcattt tttacttctt ctgtattgac ttctgataca atgtcgaagc
    87121 tagcatcttt tgccatagga accctcctga tatacatata aatatttacc atacatctat
    87181 cttatcatag gattagggca gattcttacc tgtttgctaa aattcttctc atttgagttc
    87241 gaagtatttt attttaatag ataaactgtt attcttacat ttggaatacc aaagtagggg
    87301 agaggtgctt gtgagtaaaa ttatgactgc gtatcataaa aatgaaagat taaagaaaat
    87361 agtggttatt ttgataaccg gattgaccgc tgcggtcgct ttaaattatt ttttgattcc
    87421 agccaatgtc ttttctgcag ggatgaatgg gatcgctcaa atcatttcct cgcttttata
    87481 tgaatggttt catatcaaga tagacacagg aacatttatt tttcttttga atatccctgt
    87541 gtttgtatta gggtttctca aagtaggaaa acgagcaacg atccttagtt ttgtgaatgt
    87601 tgtatgtgta tcggtgatga cgatgctgct tccagtcgga cagatcactg ataatatttt
    87661 gatgaatgcc ctagtaggcg gtgttttatt aggtcttggc gtaggattat ctttaaaaat
    87721 gggctttaca actggtggga tggatatcat ctctcttgta ctttcacaaa caactggaaa
    87781 aacagtcgga aactacatgc tgattctaaa tggtatcatt gtagtcatcg caggcttctt
    87841 ctttaactgg gaaagtgcct tgtatacgat tatttccatt tatgcaatga ctcaagtaat
    87901 tgatgccatc cacacgagtc atcaaaaagt aacagcaatg atcgttaccg tcaagccaga
    87961 agaggtaaca gaagaactag caaaacggat ggtacgtgga atgacattac tgccgtctgt
    88021 cggtggctat tcaaaaaaac aagggaacat gatcatgatg gtcatcaccc gctacgaaat
    88081 gtatgatctt gatcagatcg ttcatgaaat cgacgacgat gcatttatca atattttacc
    88141 aactcattca gtatttggcc ggtttgcaaa tgaaaacgaa caaaaaatgt atcgaacaac
    88201 tggcgtattg ccagaaataa aaaatcgttc acaaaaaaaa tgaggaatca ggtttatttt
    88261 agaataaatt taatctatta catctaataa gaacattttt ataaatttaa acgagaatct
    88321 tacttctaaa ttctcattta caagcagcta aaaaaagagt atgataaata aaaatcgtgt
    88381 taaggagtgt cgtacgatga gcgaagcaaa ccaagaacgg tctggacaag aaatagaact
    88441 aatcaaagaa cgtattcttg ctgccttgga aatggtgatt gatcctgaat taggaatcga
    88501 catcgtcaat ttaggcttga tttatgatat agaattcaat cctgaaaatg gagaaacagt
    88561 cattaagatg actctaacaa ctatgggctg tccattagca gatattttaa cagaatctat
    88621 ccatgacgca ttaaaagaag tgccagaagt aacgaaagca gaagtcaaac tagtctggta
    88681 tccagcatgg acgacagata agatgagccg ttatgcacgg attgcgttgg ggatacgata
    88741 gaaatcttgg ctgactagct atgagaagca aataggaaaa acaaaaaagc cttttatcct
    88801 ttctagaaga aaagagataa aaggtttctt tgtttataaa attttgtctg ctatattata
    88861 agttaattaa ataaatgtgt tctattgata aaggacattc aaatcccttt ccttttggaa
    88921 tccttctttt ttacatcagt ttgattttcg tatagtattc tttctcatct tcacgataaa
    88981 tcgcagcccc atttttttgt tgtgctttaa gactgccaag atttttttta tttacagaca
    89041 aaagtgcctc atcaatgccg attttttttg cctcttgtag tactaatgct aatccttgtg
    89101 tggcatatcc ttttttacga taattagggg agatgccgta accaatatgg ccagcgccgt
    89161 ttcttaatgt ttcgttcagt ttatggcgca aattaaaaat tcctacatac tcatgttcat
    89221 cactttctaa aataaagacg gttgcgggaa cgtaactctc aggaagatcg attcctaaag
    89281 acgttttttc gcattttttt acataatcta aaaattcagc aaatgtatag ccataagcat
    89341 tgttgataaa tccactctca tttggtccaa agctagtttg tagctgataa gctttcttta
    89401 aatctgcttc ccatagtttg atcagtttca tcgtattcac ctctttaagt aaccttattc
    89461 atttgttttc tactatagca ctattgttgt aaatagccat gcctattttt atttattgtg
    89521 tcatatcgta ttaagaatca tcgaacaata caaatatgaa ttacaataaa aaaattattt
    89581 gaagttcgtt cattcgttac ttatgatctc tagaagaaag aaaacgtgaa ttacttcagc
    89641 gtctagaatt ccagaaggat agtaatcaac agaagaaata atcaatatcc aaatgaaaga
    89701 agatagctga atcgagaaaa agaggaaaaa agaaatgaag aaaaaaataa aatagttact
    89761 aatcccagag tcgttcgttt aacattgaaa tcgatcatat ggataaaaca gattttttca
    89821 ccaaccctca atagatgatg gagttgctcg gatgaattat ttgtgtagat ggcataaggg
    89881 agggcgtaca gaaatgaaaa agaacaaaaa tatgttcttg acaaaaaaca catgtaatcc
    89941 taaactaaaa gatgtgatgc ggctatggcg gaattggcag acgcgctagc ttcaggcgct
    90001 agtgagggtg acctcgtgga agttcgagtc ttcttagccg cataatttgt ttttttataa
    90061 agagaaggta cacctggagc gatgagaaag aaaataacat attatctaaa agaatatagg
    90121 aaaaatttta aacaacggaa ccagattcaa ataaatcaaa aaaaatttgt gtgaaggtgg
    90181 gacagtttaa aacctaatag attgattcaa atagtagatt tatagattct gatacaagaa
    90241 atactggcaa acgatagtta aggaataaag actaaactta aactcaaaaa gaggtcatgc
    90301 gaaagaataa atcgcatgac ctcttactat attttgaatg tgtttaaacg ttcaaataat
    90361 gaatcttagt accaaccgtt tgcttcccag aatgctttcg ccgcttccca agaaccataa
    90421 cgtgaagtaa catattgttc tgctactctt tcttggtttg cagcagagta gtcaccattt
    90481 aagtaagaag aatctaattg gtaacggccg atgtaacgtc cgttcgttgc tgtgtaagaa
    90541 ccgcttgatt ctttttgtgc gatccattct tttgcagagc ttgtacttgc tggagtagca
    90601 gcagtttctg ttacagcagg agcaggttgt gtttcagcaa caggcgctgc ttgtgtttcc
    90661 gtttgaaccg tttcttgtac aacaggttgt tcagcttgaa caggttcagc ttggactggt
    90721 gcttcttgaa cggctgcttg aggttcagca gctgcttgtg cagagccttc tgttgggatt
    90781 gttaattgtt gtcctgagta gatcaggtta atatcagaaa ttgaatttga ttctgcaatg
    90841 gcattgatca aggagttatc accaacgtat ttttgagaaa tcgttgatag agtatcacct
    90901 gattgaacgg tataagcttc gtccgcatga gcagtcgttc ccatgaagaa tgcagcgccg
    90961 gcagccaaag ttgttccaaa aagtaaagtt ttaagtgatg tcatgaaaaa tctcctttat
    91021 tcgttaaaaa gttttatttg ttgtgttcaa cgaggaatac tttaacacgt gaaaatattt
    91081 cataggtaaa agaagcatga caatttatga caaatccacg ttttttgtaa cagaatgatg
    91141 ttcgctcgat gtgaaaaatg atgaggaatt tcacataatg cagaaataat gggattagaa
    91201 caggtgttct gatgagaaaa gaaatgtata aataacttga aagaaattgc ttttgttcat
    91261 cattttattt acatattgcg tatatttttt gtatttttgc ttgagatgtt acaataaaag
    91321 aaaaaggagg cggttccaat gagtaaaaga taccagaaca tcatggtagc agttgacgga
    91381 tcaagacaat cgatcaaagc gttcgctgaa gcccttgact tagcaaagga caatgaagcc
    91441 cacttattta tcgtgtcaat catcaacaaa gttgagctga cccatagtgc ctatgccttt
    91501 tcaaaaatct atgcagatga aaaacaaaga acagaagtag ccatgctgaa aaaaatcaat
    91561 gatgccaaag agtacggcat tgctgagatc cacgcaatag ttgaaactgg agatccaaga
    91621 aatttgattg ccaatgtcat ccctggacaa gaaaatattg atttgatcgt tatgggagcg
    91681 acaggaaaag gtgctataca acaagcatta gttggatcga ccgcttctta tgtcgtatca
    91741 catgcccctt gcagtgtgtt ggtcgtgaag taatgagata agggaggaat agaaaatggt
    91801 tcgtcctcaa gaagtccgtg cgccaaaaga aaaaatagaa attttagcaa ttattgaaga
    91861 cggcacacaa acaaagaagg gatactcgat cgctttagta aagtggaaag gaaaaaaagg
    91921 tgttgctctg agatgggatg gagataacca acaagataaa ggattccctg tcacagctaa
    91981 cggttatcat ccagcgtggt ttgttctgcc tgataaattt atggaattat acagctatga
    92041 ttatgcaaga gccgcagcat caatcaaagc attagataaa ttaacagaag agcagaatga
    92101 aatggaggaa aaataaaaaa gacgaaccaa tcagccttta gaaaactgat tagttcgagg
    92161 agtaaaaatg aaaaaaggtt atttgggatg aaactagaat aatgcgtata acttaaataa
    92221 aacttaaagc aataaaatgg caagagggtg aggcaaatgg aagcaggaaa agtattacag
    92281 gaagtaaaag cactatgtgc agataaaaaa tatgaagaag cgaaaagcct gatcgaaaag
    92341 aacaaagaaa atttggaaga aaagtacccg atcgcacaag aatttataga attaaaacaa
    92401 ggaacgatcc ttcaccgatt caaacatttt tttggtataa cagaatagaa aaaacgatta
    92461 aagatcggca gaaatttgct gcatctttaa tcgttttctt tttaaacatg cgagggattc
    92521 aattctgatt cttatataaa aaatagatcc aaataataag aaaaattact gaaagcgtca
    92581 attcgatgat gtgtgttatt aatacagacg ttgtttccca atgcctaaaa gaaaaaattt
    92641 gataagtaaa atatacagca ggtaaagagg ccagtaaaac tgaaaacttc ttttttgtcc
    92701 attttatgta atagaaaaga atgcttatca taataaaaaa aggaatataa aaaacaaatt
    92761 gaccgatcgc gatcatacta ttgcctccga taaataaagt ccatgataca aaataacact
    92821 ttgtgcgaaa tggcgcaagc agttttgtgg atttatccgg taaaatctgt atcgattttg
    92881 aaattagagg agaaacttgc tataatcaaa gtgaaagaag gagtgggatt ttcatggatc
    92941 atgtagtgtt agaaatcgat ttgcatttga tcaataatat tcgggtgatt tactatgttg
    93001 taagtaacag tgtcgaacaa cgtgtactga cgaataaaat aaatggtatt ttagcaaaga
    93061 aagacgttca ccgattcaat aatggtgaag gaagttatta tagtatccca gtagaaaaaa
    93121 ttatttatac gacagtcaaa gtcagagaag atctagatac caaaaaagca tatgaaccta
    93181 tttttacaac ttattgattg acagcagatg gaggaaaaaa atgaatccat atgaacaact
    93241 tattcaagat ttgatcgatg gaaaaatcga aaagattgat gtaaaaagag aagaattaat
    93301 ggcttttcga gaagcgtggc tgaagttgga gaatcgtaaa tatgtggtgg gaaaagcctc
    93361 actgaacggc aatgtgactt atcattacga tccgacacgt ttattttagt atgaagtatt
    93421 cagaaaagaa ccaaaggagg acgatgcagc agcattgtcc tcctttgatt ttacaaaagt
    93481 gaaagtttta cttatttgaa tagattttta aatttgtttt tgacactgtc gatatttaca
    93541 tcttttaaaa gattttgggc tttctcttta taaggtccta attgttcttt atgttcttcg
    93601 ataaatcgtt ttgcgccatc gaaattgccg ttcttaatca tttccttgac tttttgaaat
    93661 aattcttctt gattcatttt gaacacatcc tttgctatca acatgatttt ttacatggac
    93721 aaaacgagaa aaactgattt atccacacgt caataatatc aaataacgat catttttact
    93781 aaaaaaacac tcgattctcg cttctaaagt aggagaaaaa tctccaactt gggacggatg
    93841 gaatttcttt aactgaccca tatactgaat atataacaag gaggtagaaa gatgaaagtg
    93901 attaaatatt tgattggact ggcggcgatt agtggtattt tgctatttgg actgaaatat
    93961 tatactgaag gaagttatgg agaaatccct ggtatacttg atcagctaaa tccattagtt
    94021 gaaaaaggag aggtctatgt aaagacacaa aaaccagagg aagtaaatga atatggtaca
    94081 gcaaggtatg tgcagaaagc tgctgatgcc aatgggaaag aacgaacggt tgaatttaat
    94141 ggtctttctg tgttaaaaga aaatcattat ttgaaaatta gcaataaagg agcacacata
    94201 gaaacgtatg aggaagttcc tagagaaaaa gttccggaga aagcattagc aattattggg
    94261 taaatcagtt tttgtattaa gaatacgccc gattttatta ttcataattt aattatgtaa
    94321 actaaataca agtagtaaaa ataatacaat aatccccctg caaaaagatt gttcttttac
    94381 agggggttca ttttttcagc aagcacatca aattgggatg gcagggttcg aacctacaat
    94441 tgcttgattc agagtcaagt gccttaccag tttggctaca tcccatttat tcaacaaaat
    94501 aatcatagca ctttcctaaa aaaacgcaag ttatttttct tgatcggata gttgttgctt
    94561 tactcgtaaa aggcggcgta ctagtttctt ttctaatcca gcaggaaggg caatcagttc
    94621 tgctactgac aagacggtgt ccaataactg atattctttg acaggttcat tcataatatg
    94681 atagatgttt gctaaacgtt caatatattc aaagcgagta tactctgttc gagcaattag
    94741 aaatttcaat aaatcggtgg ctttctcgta ctcttgatct gcgtaataaa caagagattg
    94801 acgatacaat ttgatttcca gttcattgag ggtagcaggt ctgcgacgct cgttccactc
    94861 attaatagag actttacctt gaacatttcc cttgcagtca cggacaaaaa taatcggaat
    94921 ttcatttgga tccatttttt tatgtgtgaa tttttccatt atctacgctc ccttcaaagg
    94981 tttggtatct tgagtataag ggatttttgt ttgaattact agcttttctc caaagttagc
    95041 atctgtccac ccattgcagt gaaaatcgcc gaaaaatcgt tgatcggtac tgctcgattt
    95101 tttacaccat agggatcgtt gacataaata ttttctttgt catatccagt gactacacaa
    95161 gcatgaacaa gaggagtgac tcttacttct ccattatttg tggtccaagt cataaaatca
    95221 gtttcatctg ggacttcaaa attgatagtc acagaagtcc agacaggatt tcctttagat
    95281 agtgcttttt tgatatcatc gagttgagca tcggtttttg gaaaagtgtg ataatccgtt
    95341 tggacgattt gctgagcaag ggaggcaact gggggaacat agacgaccat agcccagtct
    95401 ccgccactga tatcaccaac aaatccttca ttaggatcgc catgagtccc atctgcattg
    95461 aaaacaggga cgtaatccaa ttgctcagct aactggtttt tagttgtttc ataaccataa
    95521 aaagataaaa gcatagaaag cgaagtgact tcacagccat tttctaaaga aggagaatca
    95581 aactggctct ctaatggaac atctagtaaa acttctgctt tctcttttgt ctttttcgtt
    95641 ttttgtgttg aacttttttg tgcagtgttc attgtatgtt ctggagaatg gatatgtagg
    95701 taataacttg taccgcttaa taaaagagca gctgtgcccc ctagaaacaa tagttttttg
    95761 aacgtttttt tcttcatttg aaatctccct tttcagctgt ttatctttcc tattataaat
    95821 gtatttttat tggaaatgga agtattatat tttaatatgt aggaaataat tagtttttaa
    95881 ggaatatgtt ttaatttttt gaaaaaaatt cttctaaata caacagctgt tttaattgtg
    95941 aaaacgaaaa cagtacgcta taatgcctgt aaggttgatt tttaactgta aataaaaatc
    96001 aatgagttaa atctccaaag aaatagcagt ggtaatgtgg agaaaagttc cagcagttta
    96061 ggaggatttt gatgacggaa gaaatgatta atttaggaga acaatatgca tgcaaaccaa
    96121 ttggttttac aaaaacggtc attggtgaag ttgtatcaaa aatgacaaac tgcgccgtgg
    96181 tcaaagttgc ccaatgtgct gcagaggacc aagaattatt agatgaaaaa gcaagcatgg
    96241 ttgttgcaaa atatgataca tttgaataaa gaaaacaatt ggacgtgcgg cgtgctgcaa
    96301 gtccaatttc tttgagaagg taaatcagta ttcttttcct attttctgga gaagaaaaag
    96361 cgaggcgagt tgtcatggta acattcttta cggttctatt gtttttattc gttgttgtga
    96421 tgtttttatt tctttggctg gttcgaaaag aaattattta tcggactgtg cgaaatcgat
    96481 gggtctactt ggtgataccc ttcttagtgt cactagtcat ttggtataca ctcatcagtc
    96541 atcctacagc agatgaattg gcaaaaggga tcttgtcagc aatgattttt attagttttt
    96601 tgttggacag tagaggaatc acagaagaag gacttgtttt gaatagcttt gacaaaaaag
    96661 gtgttccttt ttctgagatc aataaaattg tattatatca gccaaaaggt tctaaaatcg
    96721 taaaaatgaa ttttttccga aacggatggc gaggaccgat gctgaaattt tctgcatcat
    96781 tagaagaatt agttccattt ttgtcccaac gcttaaacga tgaggcagag attgatatta
    96841 tgattgatcc agaata
//
LOCUS       DAFBQH010000004        78975 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.004, whole genome shotgun sequence.
ACCESSION   DAFBQH010000004 DAFBQH010000000
VERSION     DAFBQH010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 78975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.004"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            14..1141
                     /locus_tag="LK694_000302"
     CDS             14..1141
                     /locus_tag="LK694_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK5051202.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1235..3070)
                     /gene="lepA"
                     /locus_tag="LK694_000303"
     CDS             complement(1235..3070)
                     /gene="lepA"
                     /locus_tag="LK694_000303"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBK5051203.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            3217..4437
                     /locus_tag="LK694_000304"
     CDS             3217..4437
                     /locus_tag="LK694_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5051204.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            4457..4951
                     /locus_tag="LK694_000305"
     CDS             4457..4951
                     /locus_tag="LK694_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBK5051205.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            4929..5615
                     /locus_tag="LK694_000306"
     CDS             4929..5615
                     /locus_tag="LK694_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051206.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(5766..8375)
                     /gene="clpB"
                     /locus_tag="LK694_000307"
     CDS             complement(5766..8375)
                     /gene="clpB"
                     /locus_tag="LK694_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBK5051207.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8626..9672)
                     /locus_tag="LK694_000308"
     CDS             complement(8626..9672)
                     /locus_tag="LK694_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBK5051208.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(9683..9955)
                     /locus_tag="LK694_000309"
     CDS             complement(9683..9955)
                     /locus_tag="LK694_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBK5051209.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(9990..10898)
                     /locus_tag="LK694_000310"
     CDS             complement(9990..10898)
                     /locus_tag="LK694_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBK5051210.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(11030..12262)
                     /gene="pepT"
                     /locus_tag="LK694_000311"
     CDS             complement(11030..12262)
                     /gene="pepT"
                     /locus_tag="LK694_000311"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBK5051211.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(12277..13398)
                     /locus_tag="LK694_000312"
     CDS             complement(12277..13398)
                     /locus_tag="LK694_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBK5051212.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(13395..14096)
                     /locus_tag="LK694_000313"
     CDS             complement(13395..14096)
                     /locus_tag="LK694_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBK5051213.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            complement(14240..14680)
                     /locus_tag="LK694_000314"
     CDS             complement(14240..14680)
                     /locus_tag="LK694_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBK5051214.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            complement(14809..15735)
                     /gene="dnaI"
                     /locus_tag="LK694_000315"
     CDS             complement(14809..15735)
                     /gene="dnaI"
                     /locus_tag="LK694_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBK5051215.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            complement(15735..17108)
                     /locus_tag="LK694_000316"
     CDS             complement(15735..17108)
                     /locus_tag="LK694_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBK5051216.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            complement(17131..17622)
                     /gene="nrdR"
                     /locus_tag="LK694_000317"
     CDS             complement(17131..17622)
                     /gene="nrdR"
                     /locus_tag="LK694_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBK5051217.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            complement(17934..18266)
                     /locus_tag="LK694_000318"
     CDS             complement(17934..18266)
                     /locus_tag="LK694_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBK5051218.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            complement(18318..18947)
                     /locus_tag="LK694_000319"
     CDS             complement(18318..18947)
                     /locus_tag="LK694_000319"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBK5051219.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            complement(18911..19747)
                     /gene="mutM"
                     /locus_tag="LK694_000320"
     CDS             complement(18911..19747)
                     /gene="mutM"
                     /locus_tag="LK694_000320"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBK5051220.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            complement(19792..22437)
                     /gene="polA"
                     /locus_tag="LK694_000321"
     CDS             complement(19792..22437)
                     /gene="polA"
                     /locus_tag="LK694_000321"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBK5051221.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            complement(22563..23246)
                     /locus_tag="LK694_000322"
     CDS             complement(22563..23246)
                     /locus_tag="LK694_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBK5051222.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            complement(23239..23724)
                     /locus_tag="LK694_000323"
     CDS             complement(23239..23724)
                     /locus_tag="LK694_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBK5051223.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            complement(24031..25365)
                     /locus_tag="LK694_000324"
     CDS             complement(24031..25365)
                     /locus_tag="LK694_000324"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBK5051224.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            complement(25496..26257)
                     /locus_tag="LK694_000325"
     CDS             complement(25496..26257)
                     /locus_tag="LK694_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBK5051225.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            complement(26273..26755)
                     /locus_tag="LK694_000326"
     CDS             complement(26273..26755)
                     /locus_tag="LK694_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBK5051226.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            complement(26775..28955)
                     /locus_tag="LK694_000327"
     CDS             complement(26775..28955)
                     /locus_tag="LK694_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBK5051227.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            complement(29099..29830)
                     /locus_tag="LK694_000328"
     CDS             complement(29099..29830)
                     /locus_tag="LK694_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBK5051228.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            complement(29899..30840)
                     /locus_tag="LK694_000329"
     CDS             complement(29899..30840)
                     /locus_tag="LK694_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBK5051229.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            complement(31020..31388)
                     /locus_tag="LK694_000330"
     CDS             complement(31020..31388)
                     /locus_tag="LK694_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBK5051230.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            complement(31999..33390)
                     /locus_tag="LK694_000331"
     CDS             complement(31999..33390)
                     /locus_tag="LK694_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBK5051231.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(33603..33989)
                     /locus_tag="LK694_000332"
     CDS             complement(33603..33989)
                     /locus_tag="LK694_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBK5051232.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(34045..34668)
                     /locus_tag="LK694_000333"
     CDS             complement(34045..34668)
                     /locus_tag="LK694_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBK5051233.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(34732..35400)
                     /locus_tag="LK694_000334"
     CDS             complement(34732..35400)
                     /locus_tag="LK694_000334"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBK5051234.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(35412..35852)
                     /locus_tag="LK694_000335"
     CDS             complement(35412..35852)
                     /locus_tag="LK694_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051235.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRARKGKGRIRVTCKTCGIKFETNV"
     gene            complement(35911..36582)
                     /locus_tag="LK694_000336"
     CDS             complement(35911..36582)
                     /locus_tag="LK694_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK5051236.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(36767..37165)
                     /locus_tag="LK694_000337"
     CDS             complement(36767..37165)
                     /locus_tag="LK694_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBK5051237.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(37162..37935)
                     /locus_tag="LK694_000338"
     CDS             complement(37162..37935)
                     /locus_tag="LK694_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBK5051238.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(37932..38615)
                     /locus_tag="LK694_000339"
     CDS             complement(37932..38615)
                     /locus_tag="LK694_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBK5051239.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(38782..39669)
                     /locus_tag="LK694_000340"
     CDS             complement(38782..39669)
                     /locus_tag="LK694_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5051240.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(39666..40535)
                     /locus_tag="LK694_000341"
     CDS             complement(39666..40535)
                     /locus_tag="LK694_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5051241.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(40672..41958)
                     /locus_tag="LK694_000342"
     CDS             complement(40672..41958)
                     /locus_tag="LK694_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK5051242.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            42231..42809
                     /locus_tag="LK694_000343"
     CDS             42231..42809
                     /locus_tag="LK694_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBK5051243.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            42809..43738
                     /locus_tag="LK694_000344"
     CDS             42809..43738
                     /locus_tag="LK694_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5051244.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            43735..45471
                     /locus_tag="LK694_000345"
     CDS             43735..45471
                     /locus_tag="LK694_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBK5051245.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(45534..46370)
                     /gene="pheA"
                     /locus_tag="LK694_000346"
     CDS             complement(45534..46370)
                     /gene="pheA"
                     /locus_tag="LK694_000346"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBK5051246.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(46367..46876)
                     /locus_tag="LK694_000347"
     CDS             complement(46367..46876)
                     /locus_tag="LK694_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBK5051247.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(46869..48164)
                     /gene="aroA"
                     /locus_tag="LK694_000348"
     CDS             complement(46869..48164)
                     /gene="aroA"
                     /locus_tag="LK694_000348"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBK5051248.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(48177..49268)
                     /locus_tag="LK694_000349"
     CDS             complement(48177..49268)
                     /locus_tag="LK694_000349"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBK5051249.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(49285..50451)
                     /gene="aroC"
                     /locus_tag="LK694_000350"
     CDS             complement(49285..50451)
                     /gene="aroC"
                     /locus_tag="LK694_000350"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBK5051250.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(50453..51517)
                     /gene="aroB"
                     /locus_tag="LK694_000351"
     CDS             complement(50453..51517)
                     /gene="aroB"
                     /locus_tag="LK694_000351"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBK5051251.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(51550..52572)
                     /gene="aroF"
                     /locus_tag="LK694_000352"
     CDS             complement(51550..52572)
                     /gene="aroF"
                     /locus_tag="LK694_000352"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBK5051252.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(53231..54205)
                     /locus_tag="LK694_000353"
     CDS             complement(53231..54205)
                     /locus_tag="LK694_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBK5051253.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(54186..56069)
                     /locus_tag="LK694_000354"
     CDS             complement(54186..56069)
                     /locus_tag="LK694_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HBK5051254.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(56087..58312)
                     /locus_tag="LK694_000355"
     CDS             complement(56087..58312)
                     /locus_tag="LK694_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBK5051255.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            58427..59461
                     /locus_tag="LK694_000356"
     CDS             58427..59461
                     /locus_tag="LK694_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5051256.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(59528..60541)
                     /locus_tag="LK694_000357"
     CDS             complement(59528..60541)
                     /locus_tag="LK694_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK5051257.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(60619..60807)
                     /locus_tag="LK694_000358"
     CDS             complement(60619..60807)
                     /locus_tag="LK694_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBK5051258.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(61247..61375)
                     /locus_tag="LK694_000359"
     CDS             complement(61247..61375)
                     /locus_tag="LK694_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBK5051259.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(61378..62751)
                     /gene="rlmD"
                     /locus_tag="LK694_000360"
     CDS             complement(61378..62751)
                     /gene="rlmD"
                     /locus_tag="LK694_000360"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBK5051260.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(62862..63917)
                     /locus_tag="LK694_000361"
     CDS             complement(62862..63917)
                     /locus_tag="LK694_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBK5051261.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(63927..65357)
                     /gene="gatB"
                     /locus_tag="LK694_000362"
     CDS             complement(63927..65357)
                     /gene="gatB"
                     /locus_tag="LK694_000362"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBK5051262.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(65354..66826)
                     /gene="gatA"
                     /locus_tag="LK694_000363"
     CDS             complement(65354..66826)
                     /gene="gatA"
                     /locus_tag="LK694_000363"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBK5051263.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(66826..67131)
                     /gene="gatC"
                     /locus_tag="LK694_000364"
     CDS             complement(66826..67131)
                     /gene="gatC"
                     /locus_tag="LK694_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBK5051264.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(67237..69273)
                     /gene="ligA"
                     /locus_tag="LK694_000365"
     CDS             complement(67237..69273)
                     /gene="ligA"
                     /locus_tag="LK694_000365"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBK5051265.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(69290..71524)
                     /gene="pcrA"
                     /locus_tag="LK694_000366"
     CDS             complement(69290..71524)
                     /gene="pcrA"
                     /locus_tag="LK694_000366"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBK5051266.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            71679..73238
                     /locus_tag="LK694_000367"
     CDS             71679..73238
                     /locus_tag="LK694_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBK5051267.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(73349..75280)
                     /locus_tag="LK694_000368"
     CDS             complement(73349..75280)
                     /locus_tag="LK694_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5051268.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(75298..76212)
                     /gene="pfkB"
                     /locus_tag="LK694_000369"
     CDS             complement(75298..76212)
                     /gene="pfkB"
                     /locus_tag="LK694_000369"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBK5051269.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(76209..76961)
                     /locus_tag="LK694_000370"
     CDS             complement(76209..76961)
                     /locus_tag="LK694_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK5051270.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(77121..77330)
                     /locus_tag="LK694_000371"
     CDS             complement(77121..77330)
                     /locus_tag="LK694_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051271.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(77341..78669)
                     /locus_tag="LK694_000372"
     CDS             complement(77341..78669)
                     /locus_tag="LK694_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051272.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            78805..78960
                     /locus_tag="LK694_000373"
     CDS             78805..78960
                     /locus_tag="LK694_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051273.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
BASE COUNT        21895 a        16996 c        14090 g        25994 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcagaac ggccatgaag gcttcttgcg gaacttcgac ggagccgatt tgtttcatcc
     1321 gcttcttccc ttctttttgt ttctctaaga gtttacgttt acgagaaaca tctccaccat
     1381 aacatttagc caagacgttt ttacgcaagg ctttgatgtc tgaacgggca acgatttttt
     1441 gtccaatagc tgcttggatt ggcacctcaa attgttggcg tgggattagt tttttcagtt
     1501 tttcaacgat tgcttttcca cgttcatagg caaagtctcg gtgaacgata aagcttaatg
     1561 catcgacttt ttctccattc agcaagatgt ccatcttcac taacttgctt ttttgataac
     1621 cagacatttc ataatccaat gaggcatagc ctttcgtgct tgatttcaat ttgtcgaaaa
     1681 agtcaaagac aatttcagaa agcggaatat tatagacgac atttactcgg taatcatcta
     1741 aataatccat cgtaatgaat tctccccgtt ttcgttgaga aagttccatt actgcaccga
     1801 caaaatcatt tgggaccatg atctgtgctt tgacgaatgg ttcttctaca tcctgaatcg
     1861 ttacaggttc aggaaaatct gccgggttat ctactgtagc agtcgtacca tccgttttat
     1921 taacatggta aataacggat ggtgcagtcg tgatcaattc tagattgaat tcccgttcca
     1981 agcgttcttg gacgacatcc atatgaagaa gtccgaggaa tccacagcgg aacccaaaac
     2041 ctaatgcttg agaggtttct ggttcgaatt gtaaagcagc atcattcaat tgcagttttt
     2101 ctaaagcttc ccgcaaatcg ttgtaacgag aagtatcaat tggataaaga ccacaataaa
     2161 ccatcggatt catttttcgg taacctggta aagcttcagc ggcaggattg tctgctaacg
     2221 ttactgtatc ccctactcgt gtatcttgga cagttttgat acttgctgtg atataaccga
     2281 catcaccgac catcaaaaag tctctagcaa ctgcttttgg cgagaagacc ccgacctctg
     2341 taacatcaaa tgttttgcca ttactcatca gtttgatttt gtctccgggt ttgaccatac
     2401 cgtccgtgat ccggacattt agaacaacac cgcggtaact atcataaata gagtcaaaga
     2461 tcaaagcttt tagcggtgca tctaagtctc cacttggtgc aggaacatat tccacgatct
     2521 gctcgagaat atcttcaatc ccaatccctg atttggcact ggctaataca gcttcactag
     2581 catcgattcc gattacgtct tcgatttctt gacggacacg ttcgggatct gctgcgggta
     2641 ggtcgatttt attgatgact ggcaggatct ccaaatcatt gtctaacgct aaataaacat
     2701 tggccaatgt ttgtgcttcg attccttgtg ctgcatcgac cactaaaacg gcaccctcac
     2761 aggcagcaag gcttcgagag acttcatagg tgaagtcgac gtgccctggg gtgtcaatca
     2821 agtgaaagat atatgtttct ccatttttcg ctgtgtaatt gagttctaca gcatttaatt
     2881 taatcgtgat tccacgttca cgttccaaat ccatcgagtc taacagctga tcttgcattt
     2941 cgcgagacgt cacagtgtgc gtcatttcta aaatacgatc ggccaatgta gatttcccat
     3001 ggtcgatatg agcgatgatc gaaaaattac gaatctgctc ttggcgttct ttcattttct
     3061 ttatatccat agcgtctcct cttttcatat aatcagcact ttccattata ccaacgaaaa
     3121 gcagtaattt aaagagtttt taattagtca gctatagaag ttttttttgt agaattttgc
     3181 tatactgtta tcaagctaat aaatggagat gaaaaaatgg atcaaaaaga ttttagagaa
     3241 aatcttgaat taaaaccggt cactcaagaa catatcgatc aattcaatga attgctttcc
     3301 tatgtgttcc aagtgactga agcagacatc gaggaaagcg ggtttgagaa taaacgagct
     3361 tttatccgtt ccaaaaaacc gattttggaa gtatcgaaag tgttcggatg gttccacgaa
     3421 aataaactga tctctcaaat cgctatctat ccttgtgaag tcaatattca cggtgcacgt
     3481 tataaaatgg gtggtgtgac tggagttgga acttatccag aatatgctaa ccatggattg
     3541 atgcaagatc taattatcgt tgcattggat aatatgcgca agaataagca atggatctct
     3601 tatttgtatc cttacagtat cccctactat cggcgtaaag gatgggaaat catgtctgat
     3661 aaactttcct ttaaaatcag agatacacag ctacctaaac aaatcccagt aagtggtatg
     3721 gttgagcgat taccagttga tcatccagac gtattttccg tttatgacaa atttgcccaa
     3781 caaaaccatg gtgctttatt ccgcagtgat tttcattggg aagagtattg gcgtttcgaa
     3841 aatgaagatg agcgcacagc cgctgtctac tacgatagca accatgaacc aagaggtgtt
     3901 ctgttctact gggtagctga agaaatattt cacgtaaaag aaatgttcta tcttgatcaa
     3961 gaagctcgaa atggcttatg gaactttatt tccgcacatt tttccatgat ctactgggta
     4021 catggagata tttataaaaa tgagccttta gcatttttga ttgaagatag tcagatcaaa
     4081 gagcagatcg aaccatattt tatggctcgg atcgttgatg taaaagaatt ccttcaacgg
     4141 tttccgtttg ttggaacagc tgatgctttt catttcatca ttgaagatcc tgtcgctcct
     4201 tggaataatg gtgtcttcgc tttaacatgg gatgaacagg gacaagtccg tgtcttaaat
     4261 gaacctatag gaaaatccgt tcgactggat atccaaacat tgacttgctt gatgatgaat
     4321 tatcgccgtg cttcttattt ggcaagaatc gaacggttag aaacagatga agaaacactg
     4381 aagtcactag agcgtatcat tccaaacatg gaagcttact ttagcgatta tttttgataa
     4441 aaaggatgat gtttagatga aaatttattt tgccgcacca atgtttgcta aaagtgattt
     4501 gttatataac caacagttag ttaaagaaat cagagagctc tcttctgagc tttcgatcta
     4561 tcttcctcaa gaaaatgaag caatcaatga caaaactgct tatgcagaca gtcgaatgat
     4621 tgctcttgct gacacagaaa aagtgttggc tagtgattta atgattgctt tactagatgg
     4681 gctgacgatc gatgccgggg tagcatcgga aatcggtgtg gcttatgcga aaggcatccc
     4741 agtgatcggc ttatatactg atactcgaca gcaaggcgga acccacccga aaaaaatcgc
     4801 tgcactacaa gaaacagctg aaaatcaatt tcactacctg aacttatata caataggttt
     4861 gatcaaattg aacggaaaag tggtttcttc agaaattgag ttgctttcag aagtaaaaag
     4921 atttttagat ggagggactt tcagtgatta aagaaatcaa aaaggtgcaa atagctttac
     4981 ttgcttttgg tgtattcgtt gtctgctata atttttatga atttatcaca caaaaatatt
     5041 cgacttctca aggaatcaca tttatcgtgg aatctttatt agggattgca ttgattttca
     5101 tgccacaagt cattttgaaa gttttcaaac ttaaaatacc agcagcaatc gttttgtttt
     5161 actggttttt cttgttcatc tctgtctttt taggcacagg gatgcatttg atcagtatca
     5221 tctctttttg ggacaaaatc ttacatgcgg tgagtccaat ggtcctgaca gctcttggct
     5281 atggattgat tggttacttg atgaaagatg ctgagatatc aaaaaccagt ccttggttgt
     5341 tcctcttatt cggctttgcg tttgctggac tttgcggcgt gttttgggag ttctgggaat
     5401 tcttatgcga ccagttctta ggcatgaatc ttcaacgttt tgctgcttct gatggcacac
     5461 tgtttgtcgg acgagcagca ttgatggata cgatgggcga cttgctgaca aatacgatag
     5521 gtgctgcctt aatgggattg tttgcatggt ctcaaagtaa aaaagatgag cgttactttg
     5581 aaagttataa attagaaaaa gtgaaaaata cctaataaaa gaggttgtga cagaagcggg
     5641 cagcttcaag aaatttcggc ttatttttcg aagaaactac ttctggaaca ctgtttattt
     5701 gaaaaaagga ggtctgacac tagttgtgtc agaccccttt tttcttttat agtctcaatg
     5761 gaagattaga cgatttcttc tagctcttct gttttgaaat gaagttgtcc atcaagtaaa
     5821 gtaatcgtta cttttgattt aggcataaca tgtcctgcaa cgatttcttt agctagtggt
     5881 gtttctactt ccttagtgat aaatcgtttc aatggtcttg caccatatgc tggttcataa
     5941 gcattttcag caatccacgt tttggcttca tcactgattg tcagcaggat ttcttgatgt
     6001 tccagacgtt gtgccaattg cgccaccatt ttgtcaacga ttcctttcac gttgtctagg
     6061 ctcaatggtg taaataaaat cgtgtcgtcg attcggttca agaattctgg tttgaagttt
     6121 ccacgtaata gcgtgttgac ttgttcagcc actgcttctg gaatcgttcc atcagcagtc
     6181 acgccttcca gcagtaactg tgaaccgata ttgctggtca tgatcagaac cgtatttttg
     6241 aaatctacta ctcgcccttt tgaatcagtc aagcgtccgt catctaggac ttgtaacaag
     6301 atattgaaga catcgggatg cgctttttcg atttcatcca acaagacgat tgtataagga
     6361 tttcgtctga cagcttccgt taattgacca ccttcttcgt aacctacata gcctggagga
     6421 gctccgacta gacgagacac agcatgtttt tccatatatt cactcatatc gatccgtacc
     6481 atatgatctt ctgagtcaaa caagttctca gctaacgctt tcgctaattc ggtttttccg
     6541 acaccagttg gtcctaggaa aaggaaagaa cctagaggac gatttgggtc ttgcaatcca
     6601 gcccgtgaac gaattaccgc gtcactgacc gcatcaactg cttcatcttg accaatcact
     6661 cgtttatgaa gtgtttcatt caatttaatt agtttttctc gttcaccttc tacaagtttc
     6721 gttacgggga tacccgttaa tctgccaacg acttgagcaa tctcattttc ggtaacggac
     6781 tcttgaacca tcttaatctc gctgtctttt gctttagctt ctaattcctt caattctttt
     6841 tctaattgag gtatcgtacc atgacgcaat acagcagcgc gttctaaatc atagttattt
     6901 tctgcatctt ctaattcgtg ttttgcttta tcgatttcag cacgttttgc tgaaacagaa
     6961 ttgacttctt ctttttctgt ttcccactgc atcttcatcg catttgcttc ttcacgaagt
     7021 tcagctaatt cttcttgcaa atttttcaag cgttttttac ttgcatcatc tgattctttt
     7081 ttcagtgcag cttcttcgat ttccaactgc atcaaacgtc tagtaacttg atctaattcc
     7141 gttggcatag agttcatttc tacccgaatc gttgcactgg cttcatcgat caaatcgatt
     7201 gctttgtccg gtaagaaccg atcagtaata taacggtcag ataaagtagc tgctgctact
     7261 aatgcgttgt catggatgtt aacaccatgg tggatctcaa aacgttcttt taatccacgc
     7321 agaatactga tcgtatcttc cactgttggt tctttgacta ataccttttg aaagcgacgt
     7381 tccaacgctt tgtctttttc catatattga cggtactcat ctaaagttgt ggcaccgatc
     7441 aaatgtaatt caccgcgagc aagcattggt ttcaacagat ttccggcatc catacttcct
     7501 tctgtttttc ctgcccctac gatattgtgg atttcatcga tgaacaagag gatacgtcca
     7561 tcactttttt tgacttcttt caagactgct tttagtcttt cttcaaattc accacggaat
     7621 tttgctccag cgatcaatgc acccatatct aatgaaaaaa tcgttttatc ttttaaattt
     7681 tcaggcacat ctttgcgtac gatacgttga gcaagccctt caacgattgc cgttttccct
     7741 acaccgggtt ctcctatcag aacaggatta ttttttgttt ttcgtgaaag aatacggatg
     7801 acatcacgga tttcttcatc acgaccgatg attggatcca tttttccgct tttgacttgc
     7861 tgtactaaat caacgccgta tttttctaat gctttgtatt gttcttcttg gttttgtgag
     7921 gtcactcttt ctcctcctct catctcttcg atgtttttac gcaattcttt ttcagataat
     7981 ccgtttttat tcaaataaac agttaacgga taatttttta atttcatcaa cgctaaaata
     8041 acgacttccg ttgaaagaaa ctcgtcgccg aagctttcac gtatttggtc tgcttcattc
     8101 aataatcgaa agaaattctg gctgaggttt tgtccatatt ggacgtttcc accagaaaca
     8161 actggatact catccagtag acgatcaatt tcatgttcaa aagattcgac atccaatccg
     8221 gcatccgtat agaaatttcg accaaaatga ttaggttgta aaaagatttt ccatacatgg
     8281 gcaatgtcga tatcttgatg ttttcgagtg accgcaatct gttgagcttc agcaatcgct
     8341 tcttgaagcg tcgtagtcat tttctcaata ttcatacagg tataccctcc taattaaagg
     8401 taagatcact tatttctttg atctttacaa aaactattat atcgctttgg tcaattttgg
     8461 tcaaaatata cacatggttt tttgactttt tttctcgact tatataaatc ttaaaaaaac
     8521 aaaattgatt gtatgtatat aaaaaaagag ccaaacaaac gtaaaattat cttatccata
     8581 acgtttttct ttgatttgat aaaaagggta gatcttataa aagacctacc cttgattttc
     8641 tttattctct ttttttactt ctattatttc ttctttgatc actcttcgcc gttcagcaat
     8701 cacatacaca aatccataga gaaatggcaa gataaaggaa ctgaaacgat acagtaaaag
     8761 cgaagaaact gcgtcaactg ttccaacaac tggtctgaat aataacaaat agacaaattc
     8821 aaaggaacca ataccagcag gcgtaggaat aacccctgaa agcactactg caaagctgat
     8881 aaacgaaatg gtcagtagaa aatctatttt aggatggttt tcaatcagaa tcaaataagg
     8941 aagtacatac caaaatacaa gttttaacat attccataga taaatccgca atagcgcagt
     9001 cctgtcttgg ataatggttt ggaccgtttc ccttaaagag tagatctgct gattgcatgt
     9061 atccacccat ttccgcaaac gctcactttt aaataactta ttcgataagc tgacaaaaaa
     9121 tatttgaaca ttcaagctga cggacatcgc caataaaaag gcaacaatca cgatcgtcaa
     9181 aaggatacca gcgatgataa accagatcat tgatggacca ttagaataaa aaagagagaa
     9241 ttggatgacc aagccagcta atgcataagt gatcactgcc agtttgtaca taatcatatg
     9301 gagtgctgtc acccctgctc cctgggatag cttcattcct tttttgttat aaaaataaat
     9361 ctccgagatc agcgttcctg taccaaacga aacgattcgg taaaacgcaa tatagctaga
     9421 ggtcaagaac ccatctttcg ttgtaaaatt cggcgcgaaa ggttcagcaa tttctttgat
     9481 tgagcgccct tcagcgattt ggtaaactgt ccctaatata acagccgctg ccaaaacaac
     9541 aacactggta gacagcaatt cggcaaagat atctgcaaat gattgattga ttaaataata
     9601 aataatggca aaaatcacaa tcagcaaaag cagattcaga atgattttag ttttcgttcc
     9661 tttagacgac atctcctgct ccttaatgga aaaaataaat gagaatcaaa tcaacaagta
     9721 cacctgcagc gaatcctata gtaaacttct ttttccgagt tttatgatgg aaaacttttc
     9781 gagagatcaa tcctcccaaa ccaccgccta acaggctgac aaatatcaaa ttattctcag
     9841 gaacacgcca gccgctagtt ctagcctgat gtttatcata ccccatcaga caaaataagt
     9901 aaaaattcac aatgaaaagg taaaaccaca aaagattgtg taacaataca tccattactc
     9961 ttcttccaac tttctgttaa ctactatttc taagcttttt cttctttctt tggtgaataa
    10021 actgttaatc cttttgatac aacagataat tcaactggaa gatcatctgt cgtgtctcca
    10081 tccgttcgag tgcccacaga tttttcctgc gcttggattt tcatgcgttt ggctgtgaaa
    10141 taagtaatgc cggggatcgt atagaatttg cctctcatga ttctaggcaa ataaaaaata
    10201 aatcggaaaa tatctaagga aggtacgatc gttacatgaa attttccatc atctagggtc
    10261 gctgagtcgt caaaatttgt aaaaccgccc acggagttag acatcgtgac tgtcaacagt
    10321 tgggattttc ctttccaatg ctcctcgtct gtttctaaat ccaaatgata ttttttcttc
    10381 ttcagcaata acttaaaaaa cttcttgata aagatccacg agccgtaacg ctgtttttct
    10441 tcttgactga tccaaactgc tgtatctgct aagattccga ttgtcagtgt agagataatg
    10501 acttcatcat tgacttgacc atagtctatt ttccgtacat tgccttccag gatcacgtca
    10561 gcagcttctt cttttttgag gggaatctca agaacacgtg caaggttgtt gacagttcca
    10621 cctggaagta atccgacatg gtattgagat aatgtttttt ggaacgcttg gacgatatga
    10681 tgaatcgtgc cgtctccacc tataattact aaagtatcag cttctatggc ttctgctttc
    10741 ttgactaatt ccgcatcatc aatcgatggg ccagtttcag ctagttctac atataattct
    10801 ggctgctttt cttttacata atcttgaaac cagcgtgcta atttctcacc ctcatctttc
    10861 cctgaacttt tgttataaaa aactagcagc ttttccattc tttacctcct atttactctt
    10921 caaaaaagag ggcgggacaa ccaattccat tgtcctgccc tttagcctta gacagatgac
    10981 attccatcat tgggacgaac gccttgtcat aagctgttct ttttcgattt taactctttg
    11041 tgttcaattc tgcaattttt acaatcacgt ctgttgcttt ttccattact tgtaaagaaa
    11101 caaattcgaa tcgtccgtgc atattttctc ctccggcaaa tagatttggc gtcggaatac
    11161 ccatgtaaga gatttttgaa ccatctgttc ctccacgaac tggttcgatc actggagaga
    11221 taccaagctc gatcattgct tgtttcgcta attccacgat gctcatatct ttttcaatga
    11281 tttctttcat gttgtaatat tgatcataca tatctacttt gattcgttct tgatcaaatc
    11341 gctgattcaa ctcttcttgg atatgtgtga tttgtgcttt gcgtgcttcg aatttttcac
    11401 gatcatgatc gcgaatgata taactcatag aagcttcttc aggattccca tccaatgcca
    11461 tcaaatggaa gaaaccttcg tatccttccg ttttttctgg aacttcatcg gctggaagct
    11521 gattatgaaa atcgattgct aactgtagag cattgatcat tgtatctttt gccgtccctg
    11581 gatgtacatt tttcccttgg atcgtaatat ttgcttgagc agcactaaac gtttcatact
    11641 gcaactcacc taaaggacca ccatcgactg tataagcaaa atctacatcg aattgctcaa
    11701 catcgaattt atccgctccg acaccgattt cttcatcagg accaaaagcc actttgatgg
    11761 taccgtgagg gatagaagga ttattgatca agatctccat tgcagtcatg atttccgcaa
    11821 tccctgattt gtcatctgct cccagtaatg tactgccatc tgttgtgatc aatgtgtggc
    11881 cttcataatt tttcaaattt ggaaaatctt ttgtattcaa tgtatagcgt tcttccgcat
    11941 ctagtacgat cgtagactcg ccatcatagt tttcaatgat ttgtggattg actcctactg
    12001 cattaaaatc tgctgtatcc atgtgggaaa taaatccaat ggaacgcact tcatgatcaa
    12061 cattgcttgg taatgttccc ataacgaatc cgttatcttc attatagaaa acatcagcta
    12121 agcctaactc ttctaattct tttttcaatt cttgcgcaaa agcaacttgt gtctgagtcg
    12181 atggagtcgt tttgcttgtt gcatcggagc gtgtttctgt tttgacatat cgtaaaaaac
    12241 gtggcaaaag attttcgtac atagtattct ctccttttaa ttaaattgaa atgggtttgt
    12301 atttgtttca gaaacgaaaa agttaatatc ccaattttct tcttgtttcc aagattcaaa
    12361 tttctcgata aatcgttgct tgcataacga ttcaatataa tgaccgggat caattgcaat
    12421 caacccagcg ctttgcatat catgagccgt atgataatag atatctcccg tgatatagac
    12481 atctgctctt tgggcgattg cttgcggata aaatttttca ccgcttccac cgcaaatcgc
    12541 aatgcgcttc acagaggatt ttgcattttt tggctggact attctcagtc catctagttg
    12601 gaacgcttct tttacttgtt ctacaaatgc ctctatagaa atttcttgcg gcaattcacc
    12661 tactcgtcct aacccaaaca tttcgactgg ttcatctata gcgaaaagat cataagcagg
    12721 ttcctcataa ggatgagctg accgcatggc ttgaatcact tgtttttcga tagtttccgg
    12781 caaaataacc tcaacttttg cctcttgaac ttgttctgtc ttccctactt tcccaatcgc
    12841 tggttgtgca ccagcttccg gagtaaatcg accgtgtcca atcgaggtaa aacttgtacc
    12901 ggtataatca ccttgtgtac cagcaccagc tgcagctaat acttcacgca ttttctgtgc
    12961 atgatccaca ggtacataaa cggccagttt tttataatga atctcatgag tctttactaa
    13021 ataactctcc acttcaatac cgagaagttc acaaaaccag tcattcagtc catcccaaat
    13081 aatatccatg ttcgtgtgtg ctgcatagac ggcaatatca tgtttcaaca aatctgcata
    13141 cattttttct tgtggctgat cagtaaccag ccgttttaca ggacgaaaga ttggaggatg
    13201 tttagcaatc agcaaatcga tttttttctt gattgcttct tcgaccactt ctggacgaac
    13261 gtctaatgtc atcattactc gttggattgg cttatccaat gttccgatat gcagtccgac
    13321 tgggtctcct tcttcagcta gccactgagg acaatagctt tcaaactttt ggatgaaagt
    13381 gcgtccgctg attgtcatgg ctttaaaacc tcctcaatca tttggatttc ctgctcgatt
    13441 tgtttttgac gtgcctcttg attagctgct tgtttgagct gttcacggat agcttttctt
    13501 tgttgaagct ctcttgtcca ttttgatttg aagatctcac ttttctcttc aagcagaaat
    13561 ggcccgaaca tcagctccat ttcattgtaa gtaactggtt tttgttcttt ttcggctaca
    13621 atgatttcat atattttttt attttcttca agtatttttt ctgattggat tttatactga
    13681 ttttcttgca gccaagatcg taatgtacgc tctccgatgt ttggctgaag gatcaaacgt
    13741 tcttgtccgc ttaagcgttg attcaacttg ccagcttcta ggatatctcg gatcagtgaa
    13801 cctcccatac ccgcaatcgt gacagctgta ataccatctt gtggctggat agcatctaga
    13861 ccatttgcaa ggcgtacggt gatacggtct tctaatccat cttttttgac ttgcttacgt
    13921 gctgattcat aaggaccttg aacgacttca ccagcaactg catattcgat ttttcctctt
    13981 aatatcaatg caacgggtaa gtaagcgtga tctgatccaa tgtctgctaa acgagcggat
    14041 tcagggacaa agtcacccac tgcggctaag cgctgagata aatctaaatg gttcatagct
    14101 tcactccttc tagataatta tagcattttc caaataaaat aaggagacta ggaataaaaa
    14161 aattcccata tctccttaaa aaatatacct tttcgttcta taaaacaaca ttttcttatt
    14221 tttgaaaaaa tgttattggt tatttgttca atgaaacatc aataatcgaa tctccaggaa
    14281 cttgcggtgg gatagacatc gtcatgatcg gcatctcaac taatgtaaat tggactttgt
    14341 caccgatttt caattcattt atgtccacgc ttattttttc agcagtagca tttaagatca
    14401 ctccgtcatt gctgaatgac gttcccatat tcgctggatc ttctttggcc atgacatcga
    14461 cgagtttcaa ctgaagcgtt tcttcttctg cttctacttg agtaatactt tcaatcgttc
    14521 cgacaaatat gctttgtctc tccgttttcg tactgcttgt ttttactgtt tgacccgcat
    14581 caatcgaatc aacagaacct gtgttgctat ctgcgctacc gcagcctgct aaaagaaaaa
    14641 atagaccaac tacagatata ctagaaaaaa tagccctcat aaaaaatcct ccgtttaagt
    14701 ttctgcatta cttgttgcgg tgattgtctc cggtcatttc tgtccatata atgattcgct
    14761 gacctgcttt tactactagg tcaaatgtca gttggacttg tcaactgttc aaccattgcg
    14821 tcggttgcga ccagtcattt cgatttcttt tgtcaaataa cgtatgcgtt ccattatccg
    14881 tttcgctttt agcggctctt catctccacg ttgagtgacg gacaaatgct gttccaattc
    14941 atccattgta aaattcgatg agaagaaggt tggtaactgt tcctgcatcc gatattgaag
    15001 gataacaccg aatacttcgt ctcgtatcca actactcatc gcatctgcac caatgtcatc
    15061 gatcatcaaa ataggtgaac gtttcactgc atctaacttt tctgccactt gatcttttcc
    15121 gattgcctgt ttcatctcaa cggcaaatgt cggaaaatgg actagcgtcg tggtgaatcc
    15181 agcaatcgct aattctctcg ctgttgctcc taataaatat gtttttccaa taccaaaact
    15241 tcctactaga tacagtcctt tatgataccc ttttggatca gcagtatatt tctctacaaa
    15301 atctagggct tctaagctcg catatcctcg tccatctgta cgttcaaagt tttcaaaaga
    15361 agccgattga atatcttttg gcatatccat tgcttttact cggcttctga tttcttcttg
    15421 tctttgccgt gctaacaatg cttccgttgg tacataagtg acatccacat aatgaaaatt
    15481 caacgtcagc ttcggctcat agccgggagc gatcatcgtt ggatcgtttt tttgatattt
    15541 acgcttttct tgcacgaatt catacaattt agcgtaactt ttttcaatat ccgcttgtgt
    15601 caattcttct tggtgtgcgc ttagaaaagc ttgaacatcc gcatcattta agacttcagc
    15661 gatcatttca gcatactttt cttgataatt gcgtttgctg atgatcctct tcaattcttt
    15721 gcctacgtct tccactagat atcaccctcc ttttttgcca ggtattcttt aaaacggcgg
    15781 tcaatctctg cttttttctc gctgctgatc tgtttttcat cttttggctg gttgacccaa
    15841 tcaggaagct tttcccgtcg aacatttcgc ttgccagttt gataatttgt ttgcttcttt
    15901 tgttttgatt gttttgcttg attacttatt tgccgaatgt gctggatcgc ttcttctgct
    15961 gtggcgattt tcttttgtgc ccattcattt gccactgtat ttacataact ggcatttaag
    16021 ctagcattat tcttgatcac tagtacataa ttcagcaaaa cattgatcac gctactggaa
    16081 agacctgatt gagaaacaag atcttgcagt aaccagtttt cttgtttaga aacatatccg
    16141 cctttttctg acttgattgc ttccagatat ttcatcggtg gaatgctctg tgcttctttt
    16201 atcagctgaa gcgctgcgtt tgtcaaatgt tcgtttgacg agggttcatt actgttttca
    16261 gaaaccgcaa ctgtttcttt tgtttgcttc accggacgtc tctgcattgc caaaatggct
    16321 ttttgcagtt ctttttcaga tactttttgt tcactaaagt cataagattg actgatcagt
    16381 tcgccaagtg tcaattcatc gaaaccgtaa agctgatgat acatgtcgac ttgtttgatg
    16441 attggatcat caagacttgc aatatgtttt tttgccagcc aatcattcaa aaactgccaa
    16501 tcgaatgtcg aatctgcttc aagtaatgct actgattcag attcttcaaa agatttattc
    16561 agcgattcga tgttttcttg attagcagcg tacactgctt cattgaagcg ataaacatct
    16621 aaaaattttt ttgtgatttt ttgatatccc tctgctgtat gccttttagg ttcaaagaat
    16681 tgggtaagct tctggaatcg tcgttcacca actttttcca gaagaagaaa agccagaaca
    16741 gagtctttga aaaatgcttt aggaatgagt ggttctaaca gctcataaat aaaacgaggt
    16801 cccagatcac tatcttcttt aaaataaaca tccagtaagc cgattccttc tagctttttt
    16861 cttgcatcat aaaattgcgg gataccgcag cctagactac taagcagatc tgcatgcatc
    16921 ccttcaggac ctttgccaga ttcttgagag atttctgata acagggtgaa atagatactc
    16981 aaggcttctc cgccgattag tggctgataa agatacagca aggcgttctt gctgtcttct
    17041 ctcagcggag ccgcttttct tccttggtaa atattttttg gttgaagctc tttccaagca
    17101 ttttccaagg aaatgcctcc tttttgtttc ttaattgttt tctttattat tctctttatt
    17161 attttctttt gctttatcca cgatatcttg caactctttt aagaaaacag acatatcttt
    17221 gaactggcga taaacacttg caaaacgaat ataagcgatt tcgtctaaat caactaaatc
    17281 ttccatcaca tattcaccaa ttagattcgt cgatacttcg ttttctccga gactgcgtac
    17341 gcggttttcg acatggttga cgatttgttc catttgttcc atagctacag gtcttttttc
    17401 tgctgaacgg atgagtcctc ttaaaatttt atcgcgattg aactcttcgc gttccccgtt
    17461 tttcttgatt actaataacg gagcagcttc aattcgttca aatgtagtga atcggaacga
    17521 acaattctca cattcgcgac ggcgacggat cgcacgtcca tcatctgctt gacggctgtc
    17581 gattactcgt gaattattat gttgacatct gggacatctc attttgtctc acctcttgca
    17641 tcgcatttta tataaaggat gtgtctttct ttacaactag ttgtattata acacgaaaca
    17701 aatcacaaac gcgactgtcc tacccaaaaa acacggatat aagaacaatt ttttctactt
    17761 tcatgcgtaa ttgtgtcccc tgtccttact aaatttcaat tttccaatac tgatcacctt
    17821 gattactgag aaataactta cacaaaagct gatttagtca tctagcacaa aaaacttgaa
    17881 gcagatgaac accttctcat ctgcttcaag tcacatctcg attttttact attttataac
    17941 ttctcttcga ttcctgtata gtgtttcatt cgctgttcct cgattttttg ctcatcggga
    18001 atgtggtcac tttctgggag cggtgtttta ctacgatcag gtgtcggatt ttttcgttct
    18061 tgagtcaaat cttgccgctt cgcttcttct tctgcctgct ggtcttctat ttcatcgatt
    18121 cttgcttggt actgcttttc tttttcttct gctgtttctg attgcgtacg accttcatcg
    18181 attagatttt ctttattgag catctcgccg attttttctt ttgcgttacc tacgacttta
    18241 tccttttgat tttttaatga tcccatcgct atccctcact ttctatatac tcaggatagc
    18301 aaaattgaca cagatactca aagaaagcga ttagcaaaaa gccaattttc gatttgtccc
    18361 acaagttttt cttttgttcc attgttgtcg aacaaaatat ccgcacgctg ctttttttct
    18421 tcgattggaa gttgactact aattctctgc tgcgcttgtt ctagcgtcag ttgattacgt
    18481 ttcatcaaac gctgaagctg agtttctggc gtagtataaa cgacagctac tgcatccata
    18541 tagtggtcat aatgaccttc gtagagcaat gggatatcta caattactaa aggatgattg
    18601 gaacttagag aggcgatttg atcttttatc gcttttcgga taaacggatc taatagctga
    18661 tcaagttttt gccgtttttc ctttgatgga aaaacaatca tcccaagttt ccctcgatct
    18721 agttgcccat caggctgcaa tacctcttgg ccaaacactg atacaatttt cctcagagca
    18781 agcgttccag gctcaacagt ttctctggca atcacatcgc catcgatcaa aggaaatcca
    18841 tgagactgaa agactttggc tgccgtactt ttaccggtag ctattccacc cgtcaaacct
    18901 aaaacaaatc ctactttgcg atttttagcc tttgacaatt cggacaatag tgcgtccccc
    18961 tttgtgccac ttttgttttc acgatcggcg ttccacaacg tatacatggt ttgccagttt
    19021 gctgatagac atgcagtgct acttgaaatc cgcctgattc tcctaatgca ttcaaataac
    19081 tgcgaatcgt ggtacctccg gcttctattg cacgatccaa aacatcaatg atcgatagac
    19141 gtaattgttc tacctctttg gggcgcagtg tgttcgccgg ttgttctgga tggatcttcg
    19201 cttcccataa tgcttcatct acataaatat tacctagacc agtcactaga cgttggtcta
    19261 gcagcaatgg cttgattgct ttgtgggact ttttcagtcc atttgcgaaa tctgctaata
    19321 agaagctatc tggcaaaggc tcaggtccca atttcatgat tcctcggtaa gttgcacttg
    19381 ctcctttttc gacgattgtc atacgaccaa attttcgtac atcgttgtag cgaagttggc
    19441 ttccatcctc aaaaaagaaa agtacatgcg tatgtttatc gattggtgta ttttctttcg
    19501 taaattgata ttttccttcc attcgcaagt gtgagatcag ttcacaatga tcgagatgaa
    19561 aaattaaaaa cttccctcta cgtccaatgg attggatggt ttcgccaaca agagaagcct
    19621 caaagatggg tgtctctggt tgctcgatga tcttgggcca caagacctga actttttgaa
    19681 tcgtttttcc tactactaac cgttccaatc ctttacgaac ggtttctact tctggtaatt
    19741 ctggcatttt ttcttttcac cttcattata ttgctctgtc ttaaacaatg tttattttgc
    19801 ttcgtaccac gtttttcccc aactgctgtc agtgatcagt ggtacatgaa gagaaacagc
    19861 ttgttccata acttccttca ccaatttatc taatgattca agttcttttt ccggtacctc
    19921 gaagacaagt tcatcatgaa cttgtaacag catcgtagct tgaagacctg tttcttttaa
    19981 tcgtttatcc aattcgatca tcgcgatttt caaaatatca gcagcacttc cttggattgg
    20041 tgtattgata gctgttcgtt ccgcaaaaga acggatattg taatttcttg aattgatgtc
    20101 tggcagatat cgacggcggt gatacagcgt ttcgacgtag ccttggtctt ttgcttcgcg
    20161 tacgattctt tccatatatt ctttgacacc tgggtatttt tcaaagtacg tatcgatgta
    20221 ttgctgtgct gcttttcggc tgattcctag attttgtgaa agtccgtaat cagagatgcc
    20281 atacacgata ccaaaattca ctgcttttgc ctgcctacgc atatttggtg tgacatcctc
    20341 tgccttttcg attccaaata cacgcatcgc tgtgctggca tggatatctt gaccttctag
    20401 gaaggcagct ttcaaatgtt catcatttga gatatgcgca agtacgcgta actcgatttg
    20461 tgaatagtca gaagaataaa tcagccagtc tttttctcta ggaacaaatg cctgacgtat
    20521 ctttctccct tcatctaatc gaatcgggat gttctgcaaa ttaggatcaa cagagcttaa
    20581 tcgtcctgtt tgggtcaatg tctggacata acgggtatgc acttttccat ctgattggat
    20641 cactttcagc agtccctcga cataggtaga ttgaattttt gcaatttgac gataggtgag
    20701 gatatcctct acgattggtg cttgttcacg aagctgttcc agtacatcta cggctgtgga
    20761 atatccagtt tttgtttttt tgataacggg taatcccatt ttttcaaata agatcactcc
    20821 taattgcttt ggtgaattca aattgaattc ttcgccggct tcttcgtaga ttttttgttc
    20881 gatctctctt aagcgagcag agaactcgcc cttcatttct tgtaagcgag tcgcatctac
    20941 tttgatcccg cttatttcca tctctgccaa aatgatagac aatggcagtt ccattttccg
    21001 gaaaagatct tcttggtttt tttctgccag ttcttggctc aattttggtg tcaacgcatt
    21061 gattgcagct acttttcgcg ctaaatgagc aaaaaagagt tcctcttctt ctggtaaacc
    21121 ttttttcgcc cctttgccgt atacagcttc atctgattgg atatcgttgt atccgtaatg
    21181 agcagctatt ccttcgatat ctgtgctttt ttcattcgta tcgagaaggt atgcacctag
    21241 caagacatca aaatcgattc ctttcgtttt tccagtatag cggtttaaag caacataagt
    21301 tcgtttcgca tcatatactt ttttcagccg agtcggatca gtgatccaag aatggaacgc
    21361 tcggttttcg aatacagata gatcgttcgt gacataaatt ttcttttctg tcccccacgc
    21421 tactccgacg atttcctctg tatgataatt gtcgcccatc atttccacgt acaacgccat
    21481 atccgtagta aacatttcct ctttgaattc atgaacgact tcaaacaaaa tatcatccat
    21541 ttttacttct tcttctacga tattcaattt agaaaggaac tgattgaaat ccatttcttt
    21601 ataaaacgga acgagtttat cgagattttt cccttcatac ttcaatgagt cgatagagac
    21661 atccacaggc gctgaagtat tgatggtcgc caattttttc gataagaaag cctgctcttt
    21721 gtcatttatc agattttctt tcattttgct ttgcttcatt tcatcgatat gttcataaat
    21781 tccttcgact gagccatatt gtttcaatag cttgatagct gttttttcac cgattttagt
    21841 cacaccgggg atattgtcag aggcatctcc ggctagtccc ttcatgtcaa taatctgttt
    21901 tggcgtcaac ccatcatatt tttccgctac atgttctggt gtatagcttt cgatatcgct
    21961 gacacctttt acagtgatat ccacttttac ttctttacta gctaattgag tcaaatcccg
    22021 atcacctgaa agcacgacga catcaaattg atctttgtct actttttctg ccaaagtacc
    22081 gataatatca tctgcttcat aattgggcaa ttcgtaatac tgtaccccta gtcctgtcaa
    22141 caaatcacgt atatatggca tttgttcttt gaattcgcca ggtgttttgg atcttccacc
    22201 tttgtactcc gcatacattt ctgtccgaaa agtcgttttt cctgcatcga acgcaaccag
    22261 aacatgagtc ggttgctctt tttgcataac attttcaaac atcgtattga atgcatagat
    22321 tgcatttgta tgaagaccgt ttctattttt gaaacgttca agagaattgt gaagcgcaaa
    22381 gaatgcgcga aaagccacac tgttcccatc aacgagtaat aatttatttt ttgtcatctt
    22441 cttaactcct tactgtaaag ttcttattca ttctaacaaa gaatcctgcg gaatgcgaaa
    22501 agaaaaagcg ggaccatacc aaataattcg gtatagcccc aactgatgaa tttttttctt
    22561 ttttagttgt tccgtccaaa gattctcaga aacgccaaga acaagttgat aaagtccaaa
    22621 tacagcttca atgccatgaa tacagcaatt ccactatgtg ctgttccgtt tgtctggaca
    22681 tagatattac ggatgctttg gttatcataa gctgtaatcc ctgcaaagat gacgactaac
    22741 agtagggaga tgaacagctc aactacgcta cttctcaaaa tgaagatgtt caagactagt
    22801 gcaatgatca ctccgattaa agctgcctgt cctgctcttc caaccccgct aagatctctt
    22861 tttgttgtta ctccgacaac agacatcccc atgaacatca aagtagctgt caagaaagct
    22921 tgcgttactg ttccaatatc gtagtatgca agagtaaccg ctaatgttat gccattcaaa
    22981 agagagtagg caataaatcc accgattgct aagctcggat tcttttgtgc ttttacgcct
    23041 agaaccatta ctaaaatcag ttcaacgatc cacagaccca caaagcctag tggaaaatta
    23101 ttgataaaag ttgctaattg caaaggaaat acccaaagag ccaagtaaga agtgactgca
    23161 ctaagggcaa gtcccatacc taaaaatcca taaacttttg cataaaaacg attgatacct
    23221 gacattgatg tacttggatt attcattcaa ttcaccttca ctttcgattg ctatttccaa
    23281 ttcaggaact tgtggcggtt ggaccataac gacagcatcc aacacacgat cttcgttcgt
    23341 attctccgct tctacagaaa tcgtcaccac atccttcatc ttatctactt ttatgacctc
    23401 aaactgaaaa gtcagtgttt cataatggaa aaccggttca ataaaattga cagaaaaatt
    23461 gacaacatgt gatcctggtc caggaagatg ctttgaaatt gcacttgtga tgatccccat
    23521 cagcatcact gatggaacga ttggctgttg atattctgtt ttttgtgcaa agtcatgttg
    23581 gatatacaaa ggatttgcat cattcgtcaa tcccaaatac agcaaaagct ggttatcttc
    23641 aatagattct gttaaggata acgagtcgcc ttcctcgatt tcatttattg tttttcctag
    23701 ttttctcggc ttacctatgt tcaagataga aaccctccag tttattttta atcatacatt
    23761 attgtaccaa aagtagcgta aaaggcaagt tctgctccct tgatttaaca gattcctata
    23821 gtttacagag gtcgtgaaaa agctgtcctg catcaaataa taagaacagc caagaaaaaa
    23881 cagcttctca tgtttttgat ttgattgggc ttattaccgc agatgcaagc ttttgaacac
    23941 cgtttatcat aagttgcaaa aaagggggct tggcaagatt tttgccactc cccctctttc
    24001 tatttttata aggacactgc agtaattgta ttacagtaca tttttcgtcg tgctgcttaa
    24061 aagtttttca tacgcttgtt caaatttttg aacatctcca gcacccatga agattactac
    24121 tgcatcatga taatctaaca atggagatac attattttct ttgatgactt ctccaccttt
    24181 tttgattttc gctccaaggt cttcaatctt aacgtttcct tgttcttccc gagccgaacc
    24241 aaaaatatcg cataagtaaa ctttatcagc taaatcaagt gcttctgcaa attcatccat
    24301 caatgcaatc gtacgcgtaa acgtatgggg ttggaaaact gcaattattt ctttatctgg
    24361 atatttttgg cgtgcgccat caatcgttgc tttgatctct gcagggtgat gcgcataatc
    24421 atcgactacg gtcatgtctg ctacgatctt ttcactgaat cgacgtttta ctcctgggaa
    24481 agtcagcatt tcttctgcaa cttctttcag atccagtttt tcgaaataag ctactgcaat
    24541 aacacctaaa gcatttaaga tattgtgttt accgaatgct ggtacagtga agtgcccaac
    24601 aaattcatcc ccatgataga catcaaaagc agaaccagaa gttgtgcgtt cgatgtttcg
    24661 tgcttggata tcatcgtttt ctgtgacacc ataataataa atcggcacat ttgcttttaa
    24721 tttgcgtaaa taagcatcgt caccatacgc aaaaatagct tttttcactt gtccagccat
    24781 tgtttggaaa gctgtaaata catcgtcgat acttgtataa taatccggat gatcaaaatc
    24841 gatattggtc atgattgcat agtctggtga ataggcaagg aaatgacggc gatattcaca
    24901 ggcctcaaat gaaaagaatt ccgcttgcgg atcaccatgt cctgttccat ctccaattag
    24961 ataacttgtt ggacggacac cactcaatac atgagagagc agtccagtcg tacttgtttt
    25021 tccgtgagag cctgttacag cgatactcgt ataattttgg ataaaatgac caataaagtc
    25081 gtgatagcga atcacttcca aacctaattc ttttgctcgc tggatttctt catgtgaatc
    25141 aggaaatgca tttcctgcaa tgatcgtcat ccctggtttt acattatctg cgttaaatgg
    25201 cagaatcgtg atatttgctt tttctaaatc tctttgtgta aagaaatatt tttcaatgtc
    25261 tgatccttgg acatttagac cttgttcatg taaaacaaga gccaaggagc tcattcctga
    25321 acctttgata ccaacaaaat gatacaattt attttgattt tccatttgaa tcctcttctc
    25381 ttatctagcg tccattcttt tagctattac attcaacacg ctcctcatta tcttataaac
    25441 gattgaaaat ttcaaccaaa tttatttgga aaggaggatt cgcttgctaa aatggtcaag
    25501 tacgagaaaa atccgttttt attgactctt taattctggt gtaagaagac caaacttcct
    25561 ctgatatatc aatgtgagga ataaaatctt ttcctttcgg accatagccg ttctcatcgt
    25621 catggagacg ttccatttct cctaatacta gcgcatggaa atattcctta ggccattgat
    25681 gattcagttc ttttgctgta aagatgatct cttcagaaaa gtgaaggatt tccggcacaa
    25741 atggcacagc attcgcgaaa atagtttgtt cattttgcca tactttctca aaagttgctc
    25801 gagtcctgcg ttgcagtgtc ggatcattca tcaataagat tttttctggt acagctccta
    25861 aggaatgaag aatctccagt gaaaaagtgg cgttctctcc agaatttgta gacttcgatt
    25921 ctatcagaaa agccttatcg ggcaaatcat agacttcttt tagatactga cgacagattt
    25981 cactttcact catgccatct tcaaaatgaa atccttgctt ttcaaaattc tcataaagga
    26041 ttctagtcgc atgccccacc cctccgacca aaaacaattg atctacttgg ccatttaaat
    26101 acatttgtgc tgctttatca gctaagatag gaagacagtt tccagctaaa ataagtgtag
    26161 gtgccgtcag ttcctcaaaa tttccctctt caggtgcggc taaaaaattt aatagctgat
    26221 tccagtcttt tagattcctc tgctcacttt tttccaaagg ccttctatcc tttcatctga
    26281 ttttttgtgc ttcattaaag tcttctcttt ttgtctcaat ccatttgatt tctccatgac
    26341 atcttccaca acgatatttg cgtacatcga ttcttctctt gcggcagatc gtggcagaac
    26401 aatcttggca tcgataatac tccatcttta tctgtttctt cgtcaaggct ggtgcatagc
    26461 gcaaccctcc tgtttttttc aaaagatact tgaaatccat atctttatgc tgataccctc
    26521 ttccagctag atgaagatga taatggcaaa gttcatgttt gatgattcct gcaatcgtct
    26581 cttcgtctga ctcttcaaat agcttcgcat taaaatctaa atgatgatcg tttaaatgat
    26641 agcgcccgcc agttgtacgt aagcgtggat tgaacattgc ttggtgcttg aaaggaagct
    26701 ggaagctttt taatgatatc tcttctacta actgctgcag ttcttcttgt gtcaattttt
    26761 gttactcctc attgttactc ttcataaggg gaaagcatcg tcaaactgat tctgcctttt
    26821 ttcgtatcga cttgttctat ccaaactgtt acgatatctc ctactgacac gacatctgta
    26881 ggatgcttga cgaatttttt gctgagtttc gatatatgga caagtccgtc ttgcttcaca
    26941 ccgatatcaa caaatgcgcc gaagtcaatg acattacgga ccgttccagt caattccatc
    27001 cctggtttca agtcttccat actcaataca tctgtacgta ataagggcgc tggcatctca
    27061 tcacgcatat ctcgtccagg ttgtgtcagc ccagctaaaa tatcggctaa cgtttcttct
    27121 ccgactgata ggctttcagc cagtttctct acggataatc gtgtcaattt ttctactgct
    27181 tcttctgtac caagctcttt ttctgaaagt tggactgaag ttaatatttc ttttgcaata
    27241 gaataacttt cgggatggat acctgtgttg tccaaaatgt ttttcccacc agggactcgc
    27301 aagaatccga ttgcttgttc atatgctttt ggtcctaagc gtggcacttt tttcaattga
    27361 gttcgtgcgg tgaattctcc gttttcttca cgataaatga caatgttttg ggcagttgtt
    27421 ttattcaatc ctgatatatg ctgcaagagc tgcgggcttg ctgtattcac atccacacct
    27481 acttggttaa ccgctgtttc cacaacaaaa tccaattgtt cagccaaacg tttttgagaa
    27541 acgtcatgtt ggtactggcc tactccgaca gctttaggat cgattttgac taattcagct
    27601 aaaggatctt gcaggcgacg ggcaatagaa acagcacttc tttcttcaac ttgtagatgc
    27661 ggaaattctt ttcgtgcaat ttcgctagcg gagtaaacag aagcgcctgc ttcgttgacg
    27721 atagcgtaat aggttttatg gtcggcggat ttcaactgct cagccacaaa aagttcagat
    27781 tcccgactgg ctgtaccatt tccgatagct accatatcta cttgatattt attaattaat
    27841 tggataaaag ctggtccagc agcttctctt tttgcttgcg ctgcaggttt atgaggataa
    27901 ataacttcga ttgctaaaac tttccctgtc gcatctacga ctgctagttt acatccagta
    27961 cgataagccg gatcaaatcc caggacaact ttacctttta gtggcggttg caacaaaaga
    28021 ttacggaggt tttcgccgaa aatagcgatt gcttgctcat ctgccttttc agtcaattca
    28081 ttgcgcagtt ctcgctcgat tgctggctga ataaagcgtt tataactatc ctgataggct
    28141 tctctgacga atgaagaaga aggagaggca ggatttttga ctatttggcg atccaagtaa
    28201 gctaaaattg ctgcttcatc tacttgaaag aatactttca gaatatcttc tttttctcca
    28261 cgattagtag ctaatattcg atgagaaacc attttatgga cgggttctgc aaagtcataa
    28321 tacatttcat atacgccttt ttcatctgct tgctcatcct tgacattgct gacatacata
    28381 cctttgttat acgtataaga ccgaatccaa gtccgaaatt ttgcattatc gctgacttgt
    28441 tcagcaataa tctcatgagc accgtgcaac gcttcttcgg cagaagagac ttctttttct
    28501 ttgtcaatat atttttctgc ttcagattgc acctctccat ctgtaagctg catcagccac
    28561 ttcgcgagtg gttcaagtcc tttttcttta gcaatcgttg cttttgtccg tcgtttttgt
    28621 ttgtatggac gatacaaatc ttccacttgt tgcattttca cggcttgggt gatttctgct
    28681 tctaactcgg gagtcagttt cccttgttca tcgattagcc gaatcacttc agttttacgt
    28741 ttttctaagt tttcaagata ggcataccgc tcttcgattt cacgaatctg gacttcatca
    28801 agacttccag tcatttcttt tcggtaacgc gcgataaaag gaacagtgtt tccttcattt
    28861 aacaaattaa gtacagtagt caactgctta ggacgatagt ctgaaagttc tttttgtaca
    28921 agctgaatga tcgtctggtt caaattttcc gtcattaaaa tgcctctttt ctgctaaaaa
    28981 ttgcttcttt attctatcat actttttatc ctgggttaaa ctactgaaag aaacaatatt
    29041 ctctataaaa ataaaaagag ccgcaacaaa gtcacagctc cttcattctt tccattagtc
    29101 agattccatg ccaaggtcct tcgttgttct gatattcaat atcttgaaga ataccttcct
    29161 tgtcaaaaac aaccccatgc aatgaccctc cgaatacagc cccaccgtcc attccgattt
    29221 tatgatcctg aatccagagg tctgttgtct gcatatctcc atggagcatt ggtgtaatcg
    29281 tatgtccaaa tacaattgtc ttacctgtat tgtttttccc ttcatgaaat ggttccctta
    29341 tccaaataaa gtctcttaga cttgtcttac gccagtcttt ctttgtcaaa tccacaccgg
    29401 catggacaaa aagataattt tcccattcat aatacaaggg gcgctggact aaaaatttaa
    29461 tcagttctga atatctcgat tggatcatca tcgagatttc agttggggag tattcctccg
    29521 atgcccctgg gtgaatcagg ctttcaaacg tttcttttcc accatttctc atataaggcg
    29581 cataccagtc ctccggtgat tgcagaaatt gcaaaaaata ttcttcatgg tttccccgga
    29641 ggtaaatggc tcctgtttct tcgactaatt gctttcctaa taaaaaacat tcttttgtac
    29701 gaggacctcg atcattgaga tcccctaata aaacaagctg atggatcttt ggatcataat
    29761 agtgaataag tttttcaaaa agctcatgtt tgccatggac atccccaata gcataaatca
    29821 atgatttcat gtggatcacc atcctcaacg ctagtatagc gcatattgga aatggtcgac
    29881 aatcaaaact attgttttct atttaattaa agctgtaaac cgcagttctg cttcttctgg
    29941 agagattcca tggtcttgaa catagcgatt gcgcggatgt ttcgcacact ctatgctaca
    30001 tgctcctaaa tgtttttctt cattttcaat ggaagcaaga atctgacgat tgcactcagg
    30061 attgctacaa ttgatatatc gttcacatgg tgttccgtca aaccagtcgc gaccgacgat
    30121 ttgtttatct acttggttga tatcgacagc gatccgttca tcaaacacat acatcttgcc
    30181 gtcccataat tctccttgga cttcaggatc cttaccataa gtagcaatac cgccatgcag
    30241 ctgtccgaca tctttgaatc cttcacggac aagccaacca gaaaacttct cgcaacgaat
    30301 gccaccagta cagtaggtca ctacccgttt ttccataaat tcttctttgt gctcgcggat
    30361 ccagtctgga agctctcgaa aactgcgtat atctgggcgt acagcccctc gaaaatgtcc
    30421 caagtcatat tcataatcat tccgtgcatc aatcaccact gtctgttcat ctaaaatagc
    30481 ttctttaaat tcttgcggtg aaagataggc acctgtcact tggtttggat caatgtcctc
    30541 ttctagcttt aaacttacaa gctctggacg gtatcttaca aacatcttct taaaggcatg
    30601 tccttcgctt ggatcgatct tgaacatcgt atcagcaaat cgaggatcag ctttcattgc
    30661 tttcatatag gcagtcgtgt tttcaacggt tccagacaca gtaccattta ttccttcttc
    30721 agctactaga atacgacctt taagatccaa agatttgcaa aaggctaaat gatttttggc
    30781 aaattcctct ggttcttcga tttttgtata attgtaataa agcagtactt gataatccat
    30841 gtatttcttc cttccttctt ttcgttccta aacagtataa agaaggcgtt cgcatttgac
    30901 gatttatttg cttgtgtata attacgcatg gaaagttttt tgtttacata atattattat
    30961 ttgaattaaa aagacttcgc tttcctgttt cgcacagaaa aacaaagtct ttatttctat
    31021 tatttacctg taaccgcaaa gtagttgcca tcgggatcag caaagttaaa gacatattct
    31081 tctccaagct gtaccatatc acctaatgtg actgattgcg tctgcatttt tttatatagt
    31141 ccaaagatat ctttactgta aaacatcaaa gatggggcac tagtagctgt gccaggtgaa
    31201 tgggactcaa caaatgctcg atcatataac agaaatgagg tttgactgtc ctcaactcct
    31261 acttcaatta taagcgtccc atccatttct tgacgatctt tttccgcaaa gccaactgct
    31321 ctccaaaacg ttgcattttt ttctacatcg tccacataaa gcatgatttt tatttcattc
    31381 gtaaacatct gtttcctcct aaacgatctg ttctaacatt ctcaactttg tcagtttacc
    31441 ataggaaaat gggaaaatct acgaaagctc tcaacagaat cgtcctactt aaatacgctt
    31501 ttctttaata ataaataagt tggttgtttt tgttatttgc gctttcactt ttcggaataa
    31561 ataatgtgta tttatatgga ataaataaaa aaagaggtcg tgactaaaaa caagtcacaa
    31621 cctctggtaa acggtgttct cctgacaact cctcggtcac aagacacaat attcaacaaa
    31681 catgagaagc tattttttga aattgctctt tgtgactcag agtgaaacgg caggctcacg
    31741 acctctttct taaacggtgt tcaaaagctt gcatctgcgg taataagctc aaccaaacgt
    31801 aaaatatgag gaactatttt tgtttggctg ttcttattac ttgatgcagg gcagcttttt
    31861 cacaacctct tattcacggt gtttcagaag tagcttctcg gaaataagcc gaaatttcac
    31921 aaaaatttga gaaactattt ttaggaaact tcttcttatt tcttaaagct gaccacttct
    31981 atcacaacct ctttttgact atgcattcac atcgatcact ttagaatcat cattgatgac
    32041 atcgcgatct aaatctttga tgcgtgcact tgtcacgata ccagctacca tgctaccgct
    32101 gacgtttact gctgtacgtg ccatatcgat taatggttca acagagataa ctaacccaac
    32161 gattgctact ggcaagttca ttgcccctaa aacgatcagt gaagcaaatg ttgcgccccc
    32221 accgactcca gctacaccaa aagaactgat tgttacaatt gccacaagca tcaaaataaa
    32281 ttccaaacta aagacattca ctccagctgc tggtgcgacg atcgttgcca acatagctgg
    32341 ataaacgcca gcacagccgt tttgtccaat agacaaacca aaacttgctg agaaattagc
    32401 tgttgcatca tctactccta aagcttttgt ctgagtctcg atattcaatg gcaaagctcc
    32461 agcacttgaa cgtgaagtaa acgcaaagct caataccgga aaggcttttt tgaaatagtt
    32521 gattggattg acttttgcac caatcaaaat cagtgagtgg acaatcaaaa cagcaagcaa
    32581 tgctacgtaa gaagcaacga caaatttacc taaattgacg atagctgcaa aatcacttgt
    32641 tgccaaaacg tttgtcatca aagcaaatac tccataaggt gtcaaacgca aaacaagcgt
    32701 cacgatccgc ataatgattt tatacagact gtcgactaaa tctgaaaaga atgctgcttc
    32761 ttttggcgct tttcttttca cgcctagata agcgattccg acaaatgccg cgaaaataac
    32821 gactccaatc gtacttgttg cacgactacc tgctaaatca gcaaaaacgt ttttagggat
    32881 aaaggataaa atctgctgtg gaatcgataa gttttcaact gtttcctgac gtgttgccag
    32941 ttcagagatt cgtgctgttt ctgcagctcc ttgtgtgaat ttagctcctt ggagtccgaa
    33001 cagcattgtt gaaccaattc cgatcaatgc tgcaatagct gttgttccaa gtaaagaagt
    33061 caatacagtc gcactaattt tccctagctt ttcagaaact ttcatttttg tgaatgcacc
    33121 aatgatcgaa ataaacacta aaggcataac tagcatttgc aagaatgata catatccttg
    33181 acctacaatc ccgatccatt ccattgattg agtaacgact ttgtcgtcta caccaaagat
    33241 caattgaagc agaccgccaa agacgatacc aactcctaat gctgtaaaga cgcgtgttga
    33301 aaattttgta tggcgctttt gcatacgata gaacacatat aataatgccg caaaaactag
    33361 aaccactaaa actgtgatga gtgttgtcat ttttctcctc ctcatattat taatgacaag
    33421 tccatcgaag gaagtaaagg ttcccgagaa tgattcttca accatcctcc tgaaaaaatt
    33481 tttttcagtg acttttatat tttagacgat ttttatcgaa atgacattct ttttgcttac
    33541 aattagtaat aaaaaaacat acctgtgata gctttaatct atcgcaggta tgtttttact
    33601 gtctattctt gtttttctga ttgaaaattc aattttaccc caggctgatc taaggcgatc
    33661 tctgtcacct cataagacat tcggcgtacg atatcgaata acggtgctac tagctcgtcc
    33721 acttcttctt gtgtgacatc attttgacct tcaattttcc gattgaggat atgattgatc
    33781 tgactcacac ttccagataa aacatatgta tcaaacacta aacggaactc taagcgtgct
    33841 cctacgatgc tatgttcttt cggatagttt tcgatcgttt cttttaaagg cgagaagcct
    33901 actttcaaat ctgtttctac ttcttggttc ggcaatctca tatcataatg aaatgcttct
    33961 acaacttctt tttgtcttct gatttccata taattttatc actcctttat gaaaattata
    34021 acatatctcc tttgaaaggg gatactactt tttaatgatg ctctgcaagg taaggatttc
    34081 attcagattt cctaccacat aatctgccgg gatctcaccc agaaaatgat cgatgtcgta
    34141 aagacaagta gcgactcctg catttttccc tgcttcgata tctaaacgac gatcgccgat
    34201 catcatcgtg tctgttcttt ctaaatgaaa agtatcgatc aaataattta aagatgctgg
    34261 atcaggttta cgaggaaagt cctgatcgat ccctactact tcctcaatca gatgtgccaa
    34321 tcgatgttct tttaacaggc cccatgttga ttctgtcaaa cggtgggtca ggatgaaatg
    34381 acgaccgccg ttgtctttta gcatttccag tacttgtttc gtttctggaa atggacggga
    34441 aacttccgta ctttcttttt catatcggtg aaataattca ttaaactctc tggcattcaa
    34501 gtttgattca ttgatcaaat aactagtaga atactttttc attttgaagt agatttcttt
    34561 tttgtccata ctgataccaa aatcttttag tgcttgccat gcaccatcta ccattgcggg
    34621 atacgtatca aacaatgtac catcaaagtc ccatatataa tttttcatgt tccttttgtt
    34681 ccattccttt gcttcttttt tctatctctc acttttagat aaaggtgttc tttaaagtat
    34741 ttttttgtcc agaattgtca aatcatctgc taatgtatac tggattggtt gtgcatttgg
    34801 gatatcgatc gtatccattt gatcttcagg aacatcttct aaatattttg tcagtgctcg
    34861 gatgctatta ccatgaccca caactagtaa atcttgccga tcttttagtt ttggtgctag
    34921 atgatcttgc cataagggaa tgatccgatt cactgtcatt tccagacttt ctccgtatgg
    34981 gatcaaatgc ggatccaagt gatcatatct gcggtcaaat ctattttctg cggttaacgg
    35041 cggaacttcc gtatatcctc tgcgccatgt ttttacttga tcttttccgt atatttcttt
    35101 catcttatct ttgtttttac ccacaagatc cccataatgc cgttcattta gacgccatgt
    35161 ttttattata ggaagccata actgacccat ttcttctagt gcgacttgtc cggtaagaat
    35221 cgcacgtttc aaaacagagg tataaacggc atcgaattga agtccggctt caagcatttt
    35281 ttgaccggca actgctgctt gttcttttcc tttttctgtt aaggcgacat ctagccatcc
    35341 tgtccagtaa ttttcaaaat ttgcttcgct ttcgccatga cgtaataata ctaatttcat
    35401 tcttttctcc cttaaacatt cgtttcgaat tttataccac atgttttgca tgtcacacgt
    35461 attcgtcctt ttcctttacg tgcacgctgc ttttgatgac aattcgggca ctcaaaaaaa
    35521 gtataacttt ttcgatccat tttgacttgc tttttgtttt ttatctgatg atatttttct
    35581 ttgactctat caagatattt caaataacgt tgattctcat ttagtctggg gtagattttt
    35641 tttgatagga aacgaaatat catccatccg aaaaagacga tgaacaacaa ttgtccgatt
    35701 ctatagggca accaacgact tagcaaacta aagacaagcg cagcgatcaa tagaccgcga
    35761 ttcaactgat caaatttcgc atatcttccc tgcataagtt tgtgatagtt ctgctgaaat
    35821 cgtattagcc tttctaacca tgtttgcatc atattatctc ctccatcatc taatatttat
    35881 tatgacaaaa aaatccaaaa agaaaaagcc ttaattggcc ttttccattg caacttggtg
    35941 aaggacattc cgatttctgg taaagtcaat cacaatcact ttctttttat cttctctgtt
    36001 gactactcga aatgtgtccg tcaatggaag aaacagcttc ttagatggta gctgttgcat
    36061 acgtataaaa cgatggatca tcgtaatcaa ttgaggaaat tgaatagacg tgtgaagcag
    36121 acctggatat tttttcaaca gctgtgtcgt tttgatcaga ttccgttttt tactgatcac
    36181 ttttaaaaag ctttttagaa tcttttgctg acgtctaaaa atagaaaaag aattttcgtc
    36241 atctatttga taacgcattt gtttttgcag ttctgctgca gtcagatgga tcttcccatc
    36301 gtttgtatcg atcattaaac cttctggaaa cagttcaacc actgctcgat cccaagattc
    36361 aagagaaagc tcgatataat cttttactgg taattccaat tctaccttaa aaaagtgcaa
    36421 aacagccgac agcccttctg cttgatagat ttctcctaat gttagcccct ctagatggaa
    36481 ctctggtgaa aaatgcagca atgttttatt ttcctgcaat tgaacatata gaaatgtaga
    36541 tcgctcaatc agagtatctt ttaaaacaac taaaaaattc atttttctcc cccttttttc
    36601 atcattttta aaatagcata ttttgaaaag aaagtctgat gcttagagta gaatttcata
    36661 aaaatttact atttcataag tattcacgag aaaaatacaa acaaaaaagc ggaaataacg
    36721 ctcatccgcc ttcttgtttg tgtttatttc ctcttcaaac gcttgtctat gattcagact
    36781 tttctctatc gttattttca tctaaaagtt cttgcattcc gataatttct cgatattgat
    36841 gaatcttagt gataccaatg actttgtcta catcaatact aaaacaaata ccattacctg
    36901 gaatatcgat gttgatgcct tgaccaatcg catccattac tttattcgtc aaactatcgg
    36961 gtactaatgt caacacaata tctttttctg gcgaaatgtc caaacctaaa aatttccctg
    37021 aatcatgtac accagaacct cttccatgta agatcgttcc gcctgctgca cctgcttctt
    37081 ttgaaaagtc gataacatct tcgcccattc ctccgtcaac gatcgtcaca atcatgtgaa
    37141 gattcaatgc tacaagatct ttcatttgct ttcccccaat ttttgcaatc tttctttttc
    37201 gattctgtct ttttcatctt ttcgtttata aatatatcct agtactaaag tggataatat
    37261 tggtgtcaat gcaatcattg caaccacacc aaacccgtca atgatcggat ctcttccttc
    37321 aatttgtgca gcgattgcca cgctcattga taaaataaat gtcgaacaca tcgggcccgt
    37381 tgctactcca ccgttatcaa aagccattgc taaaaagatc ttgtccactt ttcgtcctaa
    37441 aataaatgct agcccgtatc ctgggatcaa gaaataataa agagagaatc cgacgagcaa
    37501 acgccacata gacaatccta ctgctgcgcc gatcccaatt gaaattacca tcagcatgat
    37561 tttcgatcgg attctcccct cgctcaattc ttcaacctgt ttgatcatta catgaatcgc
    37621 cggctcagca aaccctacta agaaccccat taaaaagcca aggggaacca gtatataatt
    37681 gtaaggcaat cccgcaatag cactgcctaa atgctgtcct accggtacat aagccatatt
    37741 gactccgtgc aggaaaagga tcaacccaat agtcgttatc aagaaacctt tcataatgtc
    37801 aaagaaacgc tttttgcgca tacgaaaact aatgatattc attaaaacaa ataggattac
    37861 gataggtgcc actgccaaaa tgacttccat ggtaacgatt tcgattccat ctatcacttg
    37921 ctgccagaaa ctcattggaa gatcaccccc aaaatcatca cggcaagaat cggccctaac
    37981 gaagcaatac cgaccattcc aaaactgtca ttttcatttt tcttttgttt gatcatacta
    38041 gtcatccctc cggcaagggc caagataaat ggaacagtta ctggaccagt agtcactccc
    38101 cccgaatcaa aagcaatcgg cataaattcg gaactggtga aaaatgaggc gatcaaaacg
    38161 cctatgtagc ctatagccat caattgataa taggatagct tgaatacgac ccgaagcagc
    38221 gcaaaggcta gaaatactcc agtgccgacg ctgacgatcc caatcagcaa cactctgcca
    38281 atctctccgt cagatatatc atatacttgt tgtcccaaaa cttgcacgga aggttcagca
    38341 atcgtgatca cgatcccgat agcaaatccc aaactgacca aaacggtcaa acttttcttt
    38401 ttgatcatat atttccctac tagatctccg acttccatca tagaataatc tgctccaaac
    38461 aaaaagagcg tcattccgat catcatgatt gccgcaccaa ccagaaatga agcgaggtct
    38521 tctccttcta acggtgcaaa aacaaacgtt aaaatgacaa tcaggacagt catcggcaat
    38581 atagcagcaa tcacttcttt aaaatttttt agcatgtatc ctcctccttt actctttaag
    38641 tttaccttaa aatgaaagag ttttcgagca caaacttaaa acgttatcat ttaatttttc
    38701 tcaacataaa ataacacacg tcccaaaagt atctttttac ttggaaaaat gactttgaag
    38761 gaacgcgctt attgtgtttt tttattttac agagccgatc attccttgaa cgaattgctt
    38821 ttgcaaaaca aaaaatacga tggcagttgg taaagtagcg atgactaatc cagccatgat
    38881 cattccatag tctggtgtat aggaagcacc catagcagat aaaacaagcg gaaccgtccg
    38941 tttatcagga gattgcaaag ctactaaggg ccataaataa ttgttccagc tactcatgaa
    39001 tgtgatgatt gcagccgcgg cataggtatt tttagccgtc ggcatataca ctctaaaaaa
    39061 aatacttagt tcttttagac cgtctaatcg agcggcttct actagatctt ttggaaaaga
    39121 tttaacattt tgtcgaaaga agaagatcaa aaaagcagtg ctaacagatg gcaggatgac
    39181 aaccaagaaa ctgttgattc ccaaaaccgt tccgttcaat tgtgaaaaca ttcgatataa
    39241 agggatcatc aatgccgcaa aaggaaccat catcgatagc agtagaatat tgaaaattag
    39301 gtcttttcgt ttactttgat aaatctcaaa tccatatcct gccattgacg aaatcagcaa
    39361 tgcaaacaca gtggtcacca aagcgatcat cgctgaattc caaagggaac gagtaaaatt
    39421 cagctcattg gaaaacaaat tttgaaaatt gatgatcaag ttactgccaa tctttatttt
    39481 tcccgatgtg atatccacgg gtgtattcgt cattcccaaa accatccaga caaaagggaa
    39541 aatggaaata aatgatagga tgcttaatgc tgtatacttt aatactgacc caattgtcga
    39601 acgaagttct aatgcttgtt ttttgacgtt ggggcgttcg attgtttctt tttctaccat
    39661 atttttcatc gactttctcc tcctactttc gtttggatta tcgacaatat cacaataaag
    39721 gaaacaataa caaaggaaac tgctgccgca tatccaaaat taggcgtgaa tttataactc
    39781 aaattataga tgtattgaga tagggtggtc gttgcatttg ctggtccccc gccagtaata
    39841 ttttgtactt catcaaaaag ctgcaatgtc ccgatcgtcg aagtaatgga agtaaacaaa
    39901 atgattggtt ttaaatttgg gatcgtcact ttgataaact gctgccattt agaagcaccg
    39961 tcaatagatg ctgcttcata aatctccgaa tcaatatttt gcatgcctga aaggaaaaaa
    40021 atcatattgt atccagtcca acgccaagtg atcgaaagaa tgatcagaac tctcgcccaa
    40081 attggatgag tcaaccagcc aatcggtgtg tcaatcatac caatgcccaa caacaagttg
    40141 tttacaaggc cagattgaga aaatagactt ttcatgatca gagaataact gaccatcgaa
    40201 gtgatacatg gaagaaagat agcggttcga aacaaccctt tatatttcaa ctttttatca
    40261 tttaaaaggt tggaaataaa aagtgctaga aacaacatga tcggtacttg tacaagtaaa
    40321 tacaggaatg tattaaaaaa agctttttta aaagttgcat ctgtcaacat ccgctgataa
    40381 ttacctaacc cgttaaatgt catattgaca gggggaccac tttgaaaaga cgtgatcaaa
    40441 gccaaaacca tcggaataaa aaccatgatt gatatcaaaa tgactggcaa gattaaaaaa
    40501 gcatgcccgt ttttgaataa atctttctgc ttcattcttt tccctccaat tctatcattg
    40561 attatctaca tgaaaagaca agaaagacaa tatctgtaag gcagacgtga tcttttttgt
    40621 cttttcgttt gtctgatgaa aaagcgaaga aattcacttt tttaccaccg atcaattagc
    40681 caattgtgct tctacttgtt tttgtgtatc tgctagtacc ttgtctgtct cttctccatt
    40741 gatgattcga tgaagagctt cagctacgac tgcttcaatt gcataggtct catgtccgta
    40801 attcacttct ggtatttcag atgtccatgt tgaaaaatcg tcaaagatct tttgaccact
    40861 gtaaaattca gaagcttctt gataagcttc ttgttcattc gcgctaagca tcgtagatac
    40921 tagtccgatt tctttcgcaa gagtgcccat cagttcttca ttcgaagcaa acgttgcttc
    40981 taggaaatct ttcgctgaat cttctccagc tactcctttg atcacatacc agccagcgcc
    41041 gccaaggtta gaagcttgcg ctttttgcag attttctgga agtgcaggta ttggtgcaat
    41101 cttccatttt ccagattggt tttctgctcc ttggatcgtt gaagaatacc aagcgccagt
    41161 tgggctagaa gctactgctc ctgattgtat cgcagttact ccagcattcc agtcagaggt
    41221 ttgttcgact agatcttctt tcaaaagagt ggcaaacaat tcaagaccat actttaaaga
    41281 tgcattgtct tggatcgtga ctgtctcccc atcttcagat gtataccatt ctcctgcctg
    41341 ttgcataatc atccttactc gaccaaggtc agaaggattg acttcagcga ttttctttcc
    41401 cgtcttttct ttgacatctc gcgctacttg aatatactcg tcccacttca gattctccat
    41461 atcctcttct gtataacctg cttctgccat cagatccgta cgatagaagt ttgctgttac
    41521 gcccgaatcg aaaggaacac cgtatatttt atctcctact ttgtttaccg caaatttata
    41581 cggtgcaaaa tgatcttcct ttacaatagc agacaaatta gcgaatgcat ctggataaga
    41641 agtcaaatag ccttgtatcc tataatcttc aatcaagacg atattgggca atccttctgt
    41701 attcccgctc gctaaagcgg tgttcagttt ctgaacgata tcatcctgag acatcgtaac
    41761 aacttctatc tctgtttctt cgttctcata aacttttttt gcttcattca ctgctttgat
    41821 attgaatgtc tcatcccacg cccagacagt gactttttct tctttgtctg ctgtactact
    41881 agcacttccg catcctgtta agaaacctga tgctgccgct aaacaaacga ctccagcaat
    41941 taactttctg atgttcattt ttatccgctc cttttccatc cattagttgt ttacgtttgc
    42001 tacattcatc ataccaaaat ttgaaaacgg tttctttatt attcagatac ttcaaaaatt
    42061 tctcctaaaa taataaaaaa atacaaatca actatgacaa aaggaataat cttagcatct
    42121 agaccaaaga tcgaaatggt gaggtttctt taaaataaaa atatccttgc attttctcct
    42181 agaaataagt atttttaaat tacaagacta ttcttaaggc gggtaaaaaa atgccaatac
    42241 gcgtaattct tatcgatgat gaacagacga ttttagatgg aatgaaacag ttgatcaatt
    42301 ggtctgagca cgggtttcaa ttaataggga ctttttccag tccgctgaaa gcgcttgatt
    42361 tttctttggt acatgcaatc gatatcgtgg tcacagatgt aatgatgcct gaattaaatg
    42421 gaatcgaatt atcgcggcta ttaaaacagc aacaacctga aacacagatc cttatcctct
    42481 ctagctatga tgaatttgat atggttaaag attcttttaa agaaggcgtc gctgattatc
    42541 ttttaaagcc tcgattgagt cctgattttt ttctaaattc cttaactgcc gtttcacaaa
    42601 aaatcaaaaa aaagcccaac aaaagcccaa tcaccaatcg ttatgagcag atctgcgaga
    42661 atctttcaca gcagattgct ggacaaactc gtccattgct tgaccctctt ctttttgaac
    42721 acaccttgtt tttctgttat ataccgaaaa acggccattc agttcaaaac aaggctctaa
    42781 acaaaaacag tcgatcaccc attcattgat ggatagcgac gattcgttta ctatttatcc
    42841 tgtttctacc ttttcggaag aatccggcta tgtaatcaat actgattcca aagatacact
    42901 taggaagttt gttcaaaagt ttcgtcaaga agtttttcca ggtgattttt ttgttgtttc
    42961 tgattctttt gcaatccaag atatcaatga aatattccaa gagatcagaa attgcaccaa
    43021 aggacaacgt ttctatcata agggaaagaa ccttgcttat cagcatgaac ttcttccgtt
    43081 attaaccaac cacgagcagc aagcaagtag ttatttaaca aaaatcgttc ataaagacta
    43141 cgctttagct gtcaaagaac ttgagaattt cttcacgacg atattggaac agcctattga
    43201 tccagcttat ttgaaacatg aggcaatcaa tcgtttctat gcattactca acgctttggc
    43261 tgacgagcat gttgggaatg aagaactttc tttgctcaag atcgcccttc cttctttatt
    43321 agccgatgct gagtatttcg aagatttttc tattcttgtc aaagaaacac ttgagaaact
    43381 cacccacttt tctgcaaatg caaaaaaaga caatttcgat atcttgactt tgatctacga
    43441 gttcgtccag gaaaattatg agaaagaaat caatttacaa cttttatccg aaaaatatca
    43501 tttttcatat agctatctct cagccatctt tactgaaaaa tttggtatca acttttcaaa
    43561 atatctaaaa aaagtccgta tcagtaaagc caagctcttt ttgacggaaa cacggcttac
    43621 tttgacggaa atctgtgaga aagtcggcta taccgaactg ggttatttct ccagagtttt
    43681 taaagaagag acaggaatga caccttctca ataccgtaaa ggaacttttg taaaatgaga
    43741 aaaaaaatca aagagcatga actctttgcc aaacttctgt tcattttgtt catcggtctt
    43801 tttctgcaag ccatcgtcat ttctttgttt atttatcatc gatcaaggga tgcctatatc
    43861 cagctgttca atcaatcgaa tgatgtggta ctaaaaaaga ttcaatctga atttgaaagt
    43921 ttgaatgata caatcgagaa tacattagcc gcttttgatt ctaatccagc tgtcaaaagc
    43981 tatttttcca atgatccagc ccaacatatg gaacagcttc agcaactaag aacaatccaa
    44041 aaaatgaatg actctctttc aaaaatccat ccaatgatcg attacgatgt attgatcttt
    44101 ggagaaaatg gaagaacttt tgtcggaaac gacatgctca ctgccgtatc tgccgattct
    44161 ttttttcaat ctgcgattgc tcagagagtg aatgaacgtg ctgctgatac acaaatgctt
    44221 tttgcacacc acggattgac tcttcgcgat aagaaagcac ccagcgtctt ctttgtcaaa
    44281 aaactgaaaa atacattgaa tcacgtttac ggatatgctg ttttatcgat ctcatccaag
    44341 caactagcga atctttttca atctgtagta aatcctgaga tatcgaagat ctccttcatg
    44401 aacgaagaac agacgatcat cgcatcaaat gaagaaaata caattgggag ccgatcaatg
    44461 attttcaaaa aactaacagt aggtgaaaca acgatttcaa atggacagca gttgacccgt
    44521 cttcctctgt accgccagga ttgtacatta ctcagtcaag tcgatcttca ttctttatct
    44581 aaccaaatgg gcgtgatttt tccgattatt ttgtataata tcgtgacact tgcttttgct
    44641 ggctcattgg tatttatgta tttggatcga cacacaaaat cgatctaccg cttgattgat
    44701 tcgctaaaac ggctagagga agatggctta aaagctactg ttcctgtaca aggaagccat
    44761 gaagtccaat tgctagggaa tacgatcaat caattgctaa gtaatgctca gcatcattat
    44821 gaacagacga tccaagacga aaagaagaaa cgttcgcttg aaatccaaac tatgcaagca
    44881 cagatacagc cccattttat ttataatacg ctgactagct tgaaattttt gatttggcag
    44941 aaaaaaacag atgaagcagt tcaaggaatc gacagtttca ttactttact aagaagtacg
    45001 attgggaaaa aagaagaagt gattccagtc aaggaagaaa tcaaaagcgt acaaagttac
    45061 atcgatcttt tgagtatgcg atatggcgaa agaatctcgg ctaaaataat gattcctgat
    45121 gaactgcagt cattatctat gccgaatatg atccttcaac caattatcga aaacgcctat
    45181 cttcatgctt ttcacaataa aacgcaagga ttcatcacgg tgtacggcaa aacaaaaaat
    45241 cagcttcttt tattcgaagt gattgataac ggcgatggct tcgatactga aaataccaaa
    45301 caaaaaaatg atcatttttc aggaatcggc atcagccatg tagatgagcg gatacgccta
    45361 atgtatggcg atgcatatgg cttaacgatc caatcagtga ttgggacagg aacgactgta
    45421 acgatttgtc taccgcttct tcctaaaaat caatcagaat atcaaaaata aagaatagcc
    45481 aagcgctgat ttttttattt taagttaaat aaatcaatca gtattcttga ctttcatcta
    45541 cgcgtctctt ctactgtcag cactggataa cagcccaata tttgactttc tcctccgagc
    45601 atcttgattt cttggagcgc attctctact aatgtcattg gacggtccaa caacaaatcg
    45661 atcacgaaaa aatattctcc caagctagtt ttcaatggtc gggattcaat cttacttaag
    45721 ttgatttccc gccagccaaa agctgccagc attttatgaa gcatccctgg ccgattagca
    45781 ggtagtgtca agatgacact catttttact ggagctgact gctggctggt catctttcgg
    45841 tcaccgacga tccaaaaacg tgtctggttc aactcattat cttggatatt ctcagctaag
    45901 atctccaatc caacatgctg tgctgtttct aatgaagcaa tcgctgctgc gtcttctttc
    45961 ggatgttctg ctacatacat tgctgcagca gttgttgatt ctgttgctac taaaggaaca
    46021 ttaggataat gcgtttctaa aaactgttgt gattgtgcta gtgcttgtgg atgtgaaagg
    46081 atttttgtaa tctttgttgc tggattgcct aaaagctgct gcttgatagg aagaacgatt
    46141 tcacttttaa cttcgacttc tggatgtttg gaaagaagat cgattgtgtg atgaacagct
    46201 ccttccaatg aattctctac aggaacgaca gccagatcga cttgtttgcg aaacaaagcg
    46261 cgcaaacaaa cagggatgga agcatacgca gtcaattgtt ctgtaggaaa tagctgacaa
    46321 gccgcctgat aagtgaatga actttctggc cctagatagc tgacgttcat atgcttttga
    46381 tcctttctat aatggtatcg atgatctttt ctggtgtttg attggtcgtg ttgattcgat
    46441 gtgtcgcact ttcttcatac caagaaagtc gttgtgccaa taatgctttt atttcttctc
    46501 ttgtttttcc aagagctaaa ggtcgaatag acgtttcatc ttgttggata cgatcaacta
    46561 gacagtccgc ttctgcctct aagtaaagaa caatcggctg tgtttttaaa aaacgccgat
    46621 tttctgcact ttggacaatc cctccaccag tcgcgataat tttgtcttca tttgacagct
    46681 ttcttaaaat atctgactcc acttttcgaa agcttttttc accgtaatag tgaaaatatt
    46741 cggcaatcga caattggata tgttcctcaa tctttgtatc taaatctaca tactcttttt
    46801 gtaaacattg agctaaccct ttaccgattg ttgtttttcc agcgcccatg aaaccaatca
    46861 gcaaaatgct agccatgagc ttctcctcct gacacaagtt ctgctaaatc gtcaaaaaat
    46921 gccggataag agattgaaac agcttcagct ccttctagtt cgcaaggtcc atcagctatc
    46981 aaagcagcta cttgcagcat cataccgatt cgatgatcgc ctctgctgga cacttttgcg
    47041 ccatgcagag gtgtcggccc atggatgatc agaccgtctg ctgtcgcttt tatctttgct
    47101 cccattttct gtaattcttc tgccaccgca tcaattctgt tggtttcttt tacttttaat
    47161 tcttctgcgt ctttgatgat cgtgattcct tctgcttgag tagccactaa agctagaatg
    47221 ggcaattcat caatcagacg cggaatgatt tctccaccaa tcgttgcttt cttcaaatga
    47281 ctcgtcttga cacttaaatc agccgattgg ttctgttcat tgtgattcgt ctgggtaatc
    47341 gctgcgccca tctcctccaa cacatctaag ataccagttc ttgttggatt gaccccgaca
    47401 ttttttaaca acagttggct ttccggaaca agaagtcctg ctgctagaaa gaaagcagct
    47461 gatgaaatat cacctggaac agttatctgt tgaccagtta atttttgcgg tccggtcact
    47521 atgatcgttt tatcttccac tgtgatcctt ccgccaaatt gacgaatcat ttcttccgta
    47581 tggttacggg aacgttcttt ttcaacgatt acggatgtgc cttctgcttg taatgccgca
    47641 aacagcaaag cagatttgac ttgcgcactg gctacaggca tattgtatcg gataggtgat
    47701 aaagagcggg ttcctgtcac agtgattggt agatactggt cattttcttc tccttgcaag
    47761 tcagcgccca tctcgcgtaa aggttccatc actcgcccca ttggacgttt agacaacgaa
    47821 gcatccccga acagagtagt cgtaaaaggc tgtccagcca aaatacccat caataaacgt
    47881 atggtcgtac cggagtttcc catgtcaatt gattcggttg gttggacaaa tgagtggcta
    47941 ccgcgaccat gaatcttgat cacctcttcc tcttcttcga tctcgacacc taattgttgg
    48001 aaggcatgta aagtactcag acaatcttct gcgcgcaaaa aattttgtat agtcgtcgtt
    48061 ccttcagcaa ttgctccaaa catgatgctg cgatgggaaa ttgatttatc tgctggtatc
    48121 ctaacagtcc ctcttaatcc atgtatttgt tgtaataatt gcatttatcc agcctcctat
    48181 ttgatccagc aatgataact ggtattagac ataatgattc tttttgcttg taacaaatcc
    48241 tgttggtttt taaaactgat ttgcagtatt cctaaaatat cttccctggt ctcttggatc
    48301 ttcaaattaa tcaaagaaac tcccgctttt ccgatcagtc cagttacttc tgcaataacg
    48361 ccaggttcat ctggcacatc gataaataaa tcaaaaaagg caggaatcgc gccttgctta
    48421 tggacaggca actggtctcg actttctttt gcccgattga aaaaagaaaa gatttccgat
    48481 tggttctcag tcatgatcca ttctgctatt tgtgtcattt cttgttgcca atctgccaat
    48541 cgttccaata atatctgctt attgctcaac aaaatatctg tccacatgac aggatctgag
    48601 gaagcgatcc tagtgatatc tcgaaagcct cctgctgcca gttgtttggc tcgcggatac
    48661 tcttgattga attgttctgc ctgattgact aagccagctg ccagaatatg tggaaaatga
    48721 cttagcattc ctgttatacg gtcatgttct cttgctgtaa gagttacata ttttgctttg
    48781 gttccgcaaa atagttcttg cagtttttca acatcttttt tcgtcttttc caaaggtgta
    48841 aagatgtaat aggcattttc aaatagttta gcatctgctg cctgtatacc tgatttatga
    48901 gaaccagcca ttggatgtcc accgacaaac cgaagatttt tctgctcagc ggatgtcacg
    48961 acttgctgct tcgtactgct gacatccgta atgatgacgt gatcagacaa gttgattctt
    49021 tcaaggacat caagataagc taaggttgta cggacgggaa cggctaataa gataacgtct
    49081 gcttgttctg cacctgtttc aaaatcactt gcaacgtaat caacgatctg ctgttctact
    49141 gcttgctcac gggtttttga ctgactatcc caaccaacta ctatcagttc tggattggtc
    49201 tgacgaatac aaagtgccat agaagcacca atcagtccaa gccccacgat aaaaacatgt
    49261 tgtgccatct tatttgcccc ctttttagta atgtttcaag tagtcacggt actccttcac
    49321 tgcagctttc aactcaacaa gcgaatcact tgggaatttt gacaatagct ctgtcgctac
    49381 cacagtggct acaacatttt ccgcaacgac gctagctgca ggaactgctg tactatccga
    49441 acgttctaca cttgctttgt aaggttcttt tgtatcgata tcaacacttt gaagtggctt
    49501 gtacaatgta ggaatgggtt tcatgacccc gcgaatgaca agtggttcgc cattggtcat
    49561 tccgccttcg aagccgccta gattattggt acgtcggaaa tagcctcttt cttcttccca
    49621 taggatctca tccatcactt ctgagcctgg taatgaacca gaagtgaatc cgaccccaaa
    49681 ttctacgcct ttaaaggcat tgatactaac cacagcttgt gcgattttgg catctagctt
    49741 cgtgtcccat tgtacgtagc ttcctaatcc agctggaacg cctccaacga gcacttctac
    49801 aattcctccg atcgtatctc catttttctt tgtctgatcg atcaaatctt ttattctttg
    49861 ctcaactgct ggatcaacaa ctcgtacttc tgacgcttca gaaatttctt tgatttcttg
    49921 aatcgagata tttccaggta tttctgcttg gattccacca agacaaatca cgtgtcctgc
    49981 tacgatgatg tcaagttctt ccagaatttt cttggcaatt gcgccgattg ctacacgcat
    50041 ggtcgtctct cttgcagagg accgttcaag cacattccga agatctcgat gatggtattt
    50101 cataccgcct accaaatccg cgtgtcctgg acgcggacgt gcaactcgcc gcagtgattg
    50161 gtctttttca ggcacttctg ctacagacat gacagttgtc caatttttcc aatctttatt
    50221 ttctaccact aaagtgacag gtgaaccaat tgttttgccg tgacggatgc cagacgtgat
    50281 ccgaacacga tctttttcga tcttcatcct tcctcctctg ccgtaaccag cttgtctgcg
    50341 agcgagttcg tgattgattt cttcttctga cagaggtaat cctgctggta gtccttcaat
    50401 aatggctgtt aattctggtc catgggattc tcccgcagtc atataacgca tcttacagtc
    50461 ctcctaagta atctttgaat gtctctgtgg gaacaggaac gatttttgct tgcccaatct
    50521 tttctagcaa gatgatattc aattggcttc ctcttgcttt cttatcatgg gttatcgcgt
    50581 ggaataattc atctggatga cttagatcgt acgtaatcgg cagatggaat ttttgaagta
    50641 tcctttctaa agcagctgtc gatccagttg gagacaagcc gattttttct gcatgagaag
    50701 tgatggctac cattccaata gctactcctt ctccatgact gactacccca tatcctgctg
    50761 tattttcaat tgcatggcca attgtatgac caaaatttaa aataagtcga tttccttggt
    50821 caaagacatc ttcttccact acttttcgtt tgatttccaa cgtagcagta atgattggaa
    50881 cagcatgttc cagaagatct ttttcgtctt tcagctcgtt cagctgtttc caaagcgttg
    50941 catctgctat agctgctgat ttgatgattt cagcgattcc ttctcgtaaa cgacgtaccg
    51001 gcaaagtttt tagaacctca ggatcaatca atacaccagc tggctggcta aatgttccga
    51061 caaggttttt cgctttgttt gtattgactg ctgtcttccc gccaatacta gaatctactt
    51121 gtgcaagtag cgtagtcgga atttgaacga aatggatccc acgcatgaac gtcgaggcca
    51181 cgaatgctgc tagatcaccc accattcctc caccaagtgc aagtatccca tcgctgcggg
    51241 taaaatcatt atctgctaaa aaatcgtata actcagctgc ttgttctaaa gatttactgg
    51301 tctcacccgc cggtacgatt gaaaggactg gttcaaaatt agcttgtctc aattggaaca
    51361 tgattttttc tgcatatagt ggagcaacgt ttgtatcagt gatcacagct actttttgag
    51421 gtctccaaat cgtttgaagc catgatccag cctgttctag tgcgcctttt tcaatatgaa
    51481 tatcgtattt ttggttatca acatgtacag taagcattcg tttcacttct ttcttttaaa
    51541 aagctggaat taatcgagtg tctgaatctt ttccattgct tctttcatga tcgcaacttc
    51601 tttcatcatg cgtgagtaag tcttttcatt caacgattgg ggaccgtctg accaagcatt
    51661 tgcaggatca ggatggatct ctacgatcat gccatctgct ccgcttgcca cacccgcacg
    51721 agccattggt ggtacaagtt cccaaattcc tgtaccatga cttggatcaa caatcacagg
    51781 gaaatgagtt aattttttca tcaatgggac agcacttaaa tcaaacgtat tacgtgtcgc
    51841 tgtttcgtaa gtccgaatcc cacgttcgat aaaaatgaca ggagatttgt cttcgactgc
    51901 aatgtattct gcagcattaa gccattcatt gatcgtaccg gaaattccac gtttcaatcc
    51961 aacaggttta cctgttttac cgacagcaga tagcagttta aaattttgca tattacgtgc
    52021 gccaatctgt aggatatcgc tgtactcagc caccatatcg atatgccctt catccatcac
    52081 ttcagtaatc actttcattc caaattcatc agccgcttga cggatgtatt tcaatccttc
    52141 ttcttctaaa ccttggaaag catatggaga agtacgtggt ttgaaggcac cacctcgcag
    52201 aatttttgcg cccccggctt tggccattcg cgcacattct ctgatttggt ctaatccttc
    52261 gatagaacaa ggaccagcca tcgtcacgaa actgccgtca ccaattttta ctccgtctac
    52321 atccacgact gtattgccag gatggaattc tcgagaagtc aatttgtatg tgtttaaaat
    52381 acgcacagca ttttctactc cttcatagcg tgtaaatgct ccttcttgta aacttcgcgt
    52441 atcacctttt agtccaatca ctactcgttc tgttccttga tcgattgaga ctttgaatcc
    52501 ttctttttcg atatgatcga cgactttttt tatttgttct tccgttgctt cttttttcat
    52561 aattactatc attgtttttc ccgctttctt ttgtttactt atctatcttg gctgattttc
    52621 tctacttttt caaggtggtt ttacattaat tcctactaaa tgaccgtgtc ctgttgttct
    52681 cattttcgct ccttctttcc tacttatgac ccagataatc aacaaaaaaa gatgtccctg
    52741 taggaaattg cctctacagt gaacatcttc aacgagcctt aaaaagcgct gcaaaaatat
    52801 aaaatagcca ctatagatga atttctctac gtggctgtaa gtcgtgttta aaaatcttta
    52861 gccatcatag atacaaacct ggtttgtttg tacccacgaa gtcatgaata caaacctatc
    52921 taaaatagct aaagtaatac gtattcagtt ttgtaagttt tctagatttc ataaaaaatc
    52981 gctccaaact gatgtaagaa ttgtacctag tatagacata aaaaaatcga ctgtcaatca
    53041 tttttatgaa acttttttta attttctgat agttaacttt gtttttcaga aaaataacaa
    53101 ttggttttat tgaagaaaat agtcatgaac aaaagtatct gagaactttt ttactctctt
    53161 gttcatgaca tatagaataa agcagctttg tcttgtaaac tccgtttttc aaataataat
    53221 ctaaagattg ctactgaata ataaagccga tttcatggtc ctcttcgctg ctgattcggt
    53281 aaggtgactc aacatccgct ccccagctat tgataccccc gactcctctt acttttgcaa
    53341 aaatcgtaag aaccgttcgt ctttctggcg gtagttcttc tatatgtgtt gcctgttcca
    53401 gttcttctgc tttgaatggt aaagcggaaa aagcaaatgg cgtatcctgc tgttcaaatc
    53461 gcaacgaaaa atcttcttga tctttatccg tattatccaa cgtttgattt cttgttagtt
    53521 ctacccattt cgtttccata tgcatgccgc attcttgggg aacaaggtat ggggaaacgg
    53581 ggagtcctgc cactttataa ataccttcga caccaccagc catccgatcc ggatatgttt
    53641 cccctgatag gccttcatac tcaaaaaatt ttgctttcgt tggcataatg aaccttaaac
    53701 ccaatgcggg taattccggt aatccttttt gtccataata atgcatattg acaagcattt
    53761 taccctctcc agcgatttgg taggaaattg tcacttgggt ttttggtact gtgttgattt
    53821 ccagtgtaaa aatgatttca accgtttcag cgtattcatt acttgaatat tgattattta
    53881 ctggagcaat cggcaaatcg atctttttat tgtccaccag tacttctatt tctttgactt
    53941 ttggatacaa tccggctcct aaccacatac cggatttaaa tccaaatccg ctacctctgt
    54001 cattatcggt cgttgctcgc caaaaaactg gaagtgtttc ccggtatagc cattcttttc
    54061 cgtttttatt gagcgattcc agtccgcctc ttgtataaga aaaaatataa tgcgtattcc
    54121 ccgatttgac acctaatgta cagtcgccga agacgattgc aactttttcc gtatccatat
    54181 gacttttatt tactgtattt ttcataataa tatcttacct cctgcatggc gggtttttct
    54241 tgtccatctg caaaaacgat cccattccct gagaattcat agtcacttgg tttatcgtca
    54301 aagtcaccgc catagcgcat cacttctctg cctgtgattg gatcagtcac ttggatcgcc
    54361 tgatctttaa aatcccatat atagcctcct tggtacattt cataacgatc aagcaacgtc
    54421 atgtaagaat ccattccacc taacgaattc cccatgtcat gcatatattc acataagatg
    54481 aacggttttt ccgctttatt ttcaaggtag tcaatgatct cttctggtgg cgcatacatg
    54541 cagcttttta catctgaaat cgaatcttcg aatgctggat ttggaaaatt cccttcataa
    54601 tggacaagtc gatcggggtc tacttttttg aagtaatcgt tcatttgtcg aatgttttct
    54661 tccgcataag attcatttcc aagagaccaa aaaagaatac ttggatggtt ttttaatgtt
    54721 tcgaaattcg tttttgctcg atcaagaaca gccgcctgcc actgaggtac tgcacctggt
    54781 acgttgtaag aaggctcaaa tgcgcccatt ttttgccatg agccatggga ttcgaggttc
    54841 gtttccgcca tcacatggat tcctgcttgg tcgcataaat aataccaagt gatctggttg
    54901 gggtaatgag atgtccgtac gccgttgatc tttgcatttt ttatgatagc caaatctttt
    54961 tccatatctt ctatagtgat ggcgcgtcct tttttcggat gccactcatg tcgatttacc
    55021 ccgttgataa tcatccgttt tccattgaag tagatgattt tatctttgat ttcgatttta
    55081 cgaaaaccga aggaatagtc aatatactcc gttactcttt tttcttcgtc tatcgtctcc
    55141 aattcaagct tatataaatt gggctgaaaa tgcccccaca gtgccacatt ttcaaatcca
    55201 tcgatgcgaa tcgtcatttg ctcttttaac ggatattgct gttgatagat ttccttccca
    55261 tcaggatcta gtatcgctac attcagctga ctgtttttgg ctacttgctc aaaagacaaa
    55321 tctacttcaa gtttgccatt ttgacatttg tcatctaagc gaggtttgat ccaaagatct
    55381 tctaaatggt tttgtcctcg agcaactaag aagacatctc tgaagatccc aaaaaatcgg
    55441 aaaaagtcct gatcttccaa gaatgcagct gtacttcttt tgtaaacctc cacagctaga
    55501 atattttctt cttccaaata aggtgttaaa tcaaattcag acggagtgaa cgaatcttcg
    55561 gcatacccga taaaatgacc attcaaccaa ataaacattg cttgttctac accagcaaaa
    55621 tgaatcgaga tttgttttcc ctttagacct tcttctaatc gaaaagtcgt caaatacgaa
    55681 ccaaccggat tgtctttagc agctgaaaag atcccttccg cctctttctc agggcttgtc
    55741 gtgaaagggg gacggcgaaa tacatgtccc tcccacggat acatcgtatt gatgtaatgg
    55801 atcttgtcat aatttgctaa ttcaatatga ctaggaacag tgatttcatc aaaagaacag
    55861 gcatcaaaat cttttttata aaatgttttt ggtcgtaccg aaggatttgg cgaataacaa
    55921 aaacgccatg atccatttaa agactgttta agggaggatg tggtttctac ttcttccctt
    55981 gttgcataac aaacatgatc gctatgtgca tttgattggt tcactctaaa aacttgtgga
    56041 tcatctaacc attttatatt tgctttcata cttttttcct tctctctcat aactttttga
    56101 aatgaagcag ttctgtttgg aaatcacctt gaaaatcggg gaagtaaagt ccaactgtca
    56161 tcaattcggc ccccgaaaat atccgcttcg aggtctgttc ctgataaagt gcctcatcat
    56221 ctaaccccat cagatatact tcatggaacg ctggttgagc tgaagctagt atcctcccta
    56281 aaaacacaat agcttcatct tgtttaggag acacaaacat ccatgcggca atattttctt
    56341 caaaaggact cgccaaacga taataatggc catactggat caccggacga atcgtttgat
    56401 aggatataat ttggtctttt acttgctttt tttcttcagg agacagcttt gtcaaatcca
    56461 gttcataacc aaaaacagcg ctcatagctg tttctcctct tgttttcaac gaagtagatc
    56521 tgtcggtctg atgatttggt acagctgata catgagctgt gatggacgaa atcggataag
    56581 caaggcttgt tccatattgg attttcatac gctccactgc atccgtgtta tcacttgtcc
    56641 atgactgcgg catataataa ataaacccgg catcaaaccg tccacctcca ccagaacagc
    56701 cttcccataa gatttccgga taatcagttg tcaaatgttc aagtagttca taaagcccga
    56761 gcatataacg atgcgctact tctccttgtc tctgtgggtc aagggtaatc gaatagacat
    56821 ctgaaagcga acggttcata tcccatttga tgtagtcaag aggaatcgta tctaaaatct
    56881 ttctcatttg tttctcaatc gtctgccgaa catctaacct tgtaaaatcc agtacatgtt
    56941 gtgatctgga agcagaaggc attctacctg gctcctgcat aagaaaatca ggatgtgttc
    57001 gatacagttc agaatcaacc gagatcattt caggttcaaa ccataaacca aatttcaatc
    57061 ctttgctatg agcataatct gctatttcac gtaatccatt tgtcaacttg ccttcatact
    57121 cgaaccagtc tcctaatgaa gaattgtccg catctctttt tccaaaccag ccatcatcta
    57181 atacgaacat ttcgatgcct agctcctttg cttcatcaat gatcgccttg attttttcac
    57241 tattgaagtc aaagtaagtg gcttcccagt tattgaccag aatcggccgt tcagcaaatt
    57301 gatgacgacc tcttgcgatt ctttcacgta gcaactcatg gtggatctga ctcattttgt
    57361 tcaatccctg acttgagtaa gaaaggatca tttcaggtgt ttgaaaagac tggcctgcat
    57421 tcaattccca cgaaaaacga tggctgttga tcccaccgac gactctcaat tgattgatct
    57481 gatctttttc aagttcgaaa gaatggttgc cggaataaac aaattgaagt ccgatcgctt
    57541 ctccagtatt ttctgtcgta tgatgatgaa ctaaagcaat aaagttattc atatggtgac
    57601 tgctcgttcc tcgacggctt tcaaatactt ttgttccaga taaaacagat tgccgtgaaa
    57661 tctgtctttc attgacatgt gctcctggca aagcaatgac ttcatcaaat cgcctagtat
    57721 gggcaaagtc tagctggaaa ctagccgctt tttctagttt gattgtttct ccggtttcat
    57781 ttcttatctt tacagatcgt gtgatcactg ctctatcagt aaatatggta tagaacaaat
    57841 cgaaataaat cgctgtttct ctatccttaa gtgaaatcac caacgtttgt gcctctgact
    57901 tgtccatcac ataagtttga ggcaagcctt cgatctctgg cttcccatct gtaatataat
    57961 ggctgtcata tctcagatcc agtaagtctg atccattctt gcgctgaatg atcgatgcgg
    58021 gcgttcgaaa gtccatgctg ccatgagaag agtattcttg cggtagtgta tctttcgaca
    58081 gagaccggtc aggatttaag ggaacatttc ctgaaaatcc acgatctctt cggggataat
    58141 ttttattgtc tttgtactgc ttgactggtt tgccaaaata aatatgcgcc aatacattgc
    58201 tttcttctat ctttatcaga taagaaagaa atgtatttcg taaatggaag actcggctct
    58261 tttcatcaaa taagatgaaa tttttatctg tttttctatg cgtaatatcc atcatttcac
    58321 tcccaacaaa acggaaaccg tttacttttt tttataattc ctgtatactg tttactaaac
    58381 aattttgtca ataaaaattt tttatttttt tgaggagtgt gtaaaaatgg ttggaattag
    58441 agatgtcgca cgaaaggcaa atgtctctcc tgctacagtt tcccgtgtgt tgaataacga
    58501 tcagacgatc catgtggctc agactactcg gcaacggata gtagaggcag ctgctgcgtt
    58561 ggattatgac atcagtactc gtaaatatat caaaaagaga atgccttcga ttggtgtgat
    58621 ctctacgatc ggcaaagcga gcgaagaaca ggatatctat tacaaacatt tgcgagtagg
    58681 gatggaagaa gaagctcgac gtctgcattt agggatgaat cgaatctata atctatctga
    58741 taatcctaaa caatgggaag atctagatca gttaggtgcg gtgatcgttg ttgggactgt
    58801 tacgaaagaa tcgattaccg ccttgttaaa acaaaacatg caaatagtcg tcgtggataa
    58861 ccctgacatc cagcaagagg tcgatatggt gtatgcagat cttgaacgga tgactaaaac
    58921 cgtactggaa ttgttcttag aaaatggtca taaaagaata gcctatatcg ggggatataa
    58981 agtcgatatg gatgaattcg gtgggaagca gatttctata aatgaaaaga ggatgcgtgc
    59041 ctatcagcat tttatgatcg atcatcactt agaaaaatat atgtcttatc atctaggtgg
    59101 ctggacagaa aaacaaggtg aggtacttgc gactaatcta ttagaaaatg aatcccctct
    59161 tcctactgcc attcttgtcg ggagcgatcc aatggcaatt ggtgtctatc gggcattgaa
    59221 acaaaaaaat atccaaattg gagaagagat tgttgtagca agctttgata atatcgagac
    59281 aatcgcaaac ctctcccctg gcttgaccag tgttcaaatc aatgcgaaat caattgggca
    59341 agcagctgtt cgaatggcat tggagcgaat tgacggtata cgggacgaac caatcatttt
    59401 gacttatcct gcacgattga ttgttaggga aagttttatc ccaagaacaa aaaggtattg
    59461 aaagagaggt tgtgaaaagc cgcttcgttt cttcatactc aaaacgaaac ggctttttca
    59521 caacctctta cttatactta ttcacaaaat ccaatgtctg ctggtaacaa ttttgcgatt
    59581 cttttagcgt gtaatcaaat tggtattcat gggtaatctc tttttttgta tctgtataaa
    59641 acaaggattg cacgggtacc tttaattctg tcaaccgatt ttctaatgct tgaccttgct
    59701 cctggaatga gtatgcattt ccgtcagtta tatacgttgg cggaaattcc ttagaaacat
    59761 ggtcagccac actagcttct ttcaattgct cactcgtttt ccaatctttt gttccaatca
    59821 acgaccacgc aacggttttg acgaaaaact tcataaatcg atcatccgaa tgctgctgtg
    59881 ctgttagctt caaatcaaca ggtccgcaat aagaaatggt tcctttcaga ctatcttttg
    59941 gaagtagctg tttgattttc atttgtttag catacgcatc attcgtttgt atagctgcat
    60001 attgcaatgc gatttgtgaa cctgcactat ctccaccaaa aaataattgt tccatgtcta
    60061 gcaatttgtc gtctttttct tcagctaata aatctttgat cagttcattc acttgaagaa
    60121 cttgattagg gtattctgaa tcaggcgcta attcataatt catcgcaacc actgcgatgt
    60181 tggcgtctga aactaatttt gtcgcaaatt ctttgacacc cgatttatct ccgccgacga
    60241 atcctccacc atgtacccaa aatagaacag gaacaggccc cttgctgttt tttggatagt
    60301 agatgtcata agtattctct ttatacttgg actcatacgt caaatctgtt tttttcgcca
    60361 cgtttttttc tgcttgatcg aacgtttttt tatcagtgat ttgaacagta tttgaaaaca
    60421 tatggttgat caagaatgct ccaggtctgg gggaaagctg gaaggcaaga aagattgcaa
    60481 acgcgatccc aagaatgatt ccgaagaacc atccgataaa tttaagaatt ttctttttca
    60541 taaggctccc tcagtagtta gtattaagca ttctgattat accatagtgt tatttcctat
    60601 caagctctcc tagatgattt aatgattttc cccaaacttc ttctgcaatg cttttgacaa
    60661 aagaaatttt ttccattgtt cttcaaacaa aacattttct tcttctgttg aagcaaatcc
    60721 acattgagga cttaagcaca aacgatccaa aggcagatat cgtgtcgctt cgtagatccg
    60781 gtcaatcact tgctggtgct cttccatttc gccatttttc gttacagtca aaatctaaga
    60841 caactaattt atcttcagag actttctcta aaagaacaaa actcccagca cgttcactat
    60901 cgtattctaa ataataggca cggacatttt cttgagtaaa ataatgggtc tgctacgtcg
    60961 tcatcccccc tgatgccgcc aaaatcgaat aataatttcc gcgtcaaaca tgtatattta
    61021 gtgttgaatc atttgagaat atgaaaataa aaatcttttt gatcaatcag ttaatcaaat
    61081 tgaaataacg aaaaatctga gacagaatga cgttcattct gacccagatt tgattttgca
    61141 caactcaaca gtccttattg gcaaatcgcc aaaataatgt agactaacta aaaagactaa
    61201 gaaacaataa caaaagttaa cacttcttag tctttcttta gcacgtctat ttattaaaga
    61261 ttttagggcc ttttctcatg tttttacgat cattcttagg tgtttccatt tgtttggatc
    61321 ggcctccacc ttgtttcttt ttgatcaatt ctttcatttt ttctttttgg tccattttta
    61381 ttctccttta acgaatgaag cgactaattc gatatgatgg gtttgcggga aaagatctac
    61441 aggctgaact ttcttcacgg cataacctgc ttctgcaaat aacttagcat ctcttgcaaa
    61501 agttgccgga ttacatgaga tatagacgat tcgttctggt gacatttcga ttgctgattc
    61561 aataaaatga tgatctaatc cttttcgtgg agggtcaact acgatcacag atggtttgat
    61621 ccctttttct tgccattttt tcataacttt ttctgctttt ccgacttcat aatgaacatt
    61681 ttcgatttta ttatttaaag cattgaactg tgcatcttca attgcttctg gaataacttc
    61741 catcccatac accttatcca ctctgtctgc taaagacaag ccaatcgtac ctattcctga
    61801 ataagcgtcg atcacaatgt cttttttatc taactcggca aactcgatgg ctgtttggta
    61861 cagatactct gcttgttgag tatttacttg ataaaatgac tttgctgaga ttcgataggt
    61921 ttttcctaat agctgatctt cgatatagtc tctgccatat aaaacttttt ctttgtctcc
    61981 taaaatcaca tttgtttttt cttcattgat attttggatg accgaaacca cttctggcaa
    62041 ttcttcatga atagcttcag caatttcctt ggatttaaag aatttttctt tacgagtaac
    62101 taaaacgacc atcatttcat gggaataatg accacgacgg ataatgatgt gacgaagctg
    62161 tccttcattt ttttcttcat tatatcctcg tacttggaac cgttgcaaga tatctctgac
    62221 tactttgatt gcttgatcga tgacaggatc ttggatataa aaatcttcga tagggactag
    62281 atcatggcta ttctttttgt aaaaaccggt ggctaactgt ccatcgatac ggcgaacagg
    62341 gatctgtgct ttattacggt agccgaatgg ttctgccatt ccgaccgttg gaagtacagg
    62401 aacttctggc atcttagcaa tcttcgccat tacatgctct acttgttttt gtttgaagac
    62461 aagctgttgt tcatacttca aatgggctaa aggtgcgata cctatgcgaa gtaatgcatt
    62521 gttttctaca ggctgacgat caggtgattg tgtaataaat gtttcgactt ttccatatcc
    62581 aaacttattg cctgctttta ctactagtat ttctacttct tctccaggga gtgcattttc
    62641 gataaataat ggataaccat cgatttttgc tacccccatt ccttcataac tcaagtcgac
    62701 gatttcgact gtatatcgtt cgtttttctt tactatttgc tgttctgtca tattttgctc
    62761 ctttaagcct atttttcaat cctactgaac tctactataa atcagctaat tacacaacta
    62821 aaaccggcac tgaccttact acgttcagca ccggcttttg attaattctt ttgattaggt
    62881 gtttcgtctg ctatttttcc gtcaccgtcg atatcttcat ccgttaaacg ttcgacttct
    62941 tttacgaatt ctttgctgac aacctctaat tcttcttcat ctgtacctgt gattgcttca
    63001 tcaggaatct gatcggtatt ggcaaagaac tcaatgtgtt gatgaagatt ttggaaatgc
    63061 atcggtgctt ccccgccgta ttcaccatct aagttgatca ttactggtcg attgatatca
    63121 tcttctgttt caacgaacaa actgcttgtt ttggtataga ttattcttgg atcatcgaca
    63181 tgtcgaccgc cattcagcat gagtgctgca atatgcaaga tctcaaagat atttgctgtc
    63241 ttcacaatga ttaaagaaaa cttcccatca tccaatttgg catctggcgc gatctgttcg
    63301 aatcctccaa cagagttcgt cagccctagg aaaaacatcg aagcatttcc gatatattcc
    63361 ccttcatcat acttcaagcg cattttgatt ggctttactc gagggagcat ctctgctcct
    63421 ttcgcaagat aagcaagata accaaaaata ctctttagtt ctgatggaac ttcataagtc
    63481 aattctgtca aatagccacc tgcagcgata ttgatgaagt aactgttatc agaaaggccg
    63541 atgtccattt ttacggtttg gtgttttttt atcacttctg ctgctgcttt aatattatct
    63601 ctaggaattt ttaatgctcg agcatagtcg ttcgttgtac cagctgggat gatggccatt
    63661 ttaggacggt gttcaagcgg agcgattcca ttaaccactt cgttgattgt gccatcgcca
    63721 ccagctgcca ctactagatc aaaacctagt tctgcgactc tacatgcttc attcttggct
    63781 gaatctggtt ctggtgtagt tgcataggcg ctggcttcaa atcctgcttc ttctaatgcc
    63841 tgtaggatat ccgccaaatt ttttttcaat aattcttttc ccgaggtggg gttataaatt
    63901 acgcgtgctt ttttcatttt cagcctctat cgtttcgcta attcatcttt aagtagctta
    63961 ttcacgacac caggatttgc ttgtccttta gttgctttca tgatttgacc gactaggaag
    64021 ccaaccgcac gatcttttcc atttttgaaa tcatcgacag attgttggtt gttatccaaa
    64081 atttcattaa tgattggcag tagtttgttc ggatcagata attgcaccca accatttttt
    64141 tcaactactt cttgggcttc tccgccattt gtgatcaatt cacggaagac ttttttggcg
    64201 atttttgagc tgatcgttcc atcggcgatc aaacgaatca ttcctgctaa gttctcaggt
    64261 gtcaattttg tctcagctaa ttccagtttc tcactgttca agtaagcaga aacttcaccc
    64321 atcaaccagt tagatgcttg cttggcatct gcaccatttt ccaaaacagc ttcaaaaaaa
    64381 tcagacattt cttttgtaag tgtcaatacc attgcgtcat actctggaag tccaaactca
    64441 cggatatagc gttcgcgacg agaagctggc atctcaggca aactagcttt tactttttga
    64501 atccattcgt catcgatcac gaatttcggg atatcaggct ctgggaagta acgataatca
    64561 ctagaccctt ctttgacacg catcaaaact gttttattcg ttgtttcgtc aaaacggcgt
    64621 gtttcttgtt ggatttctcc tcctgaaaga agaacttttg cttgacgttt ttcttcaaaa
    64681 gctaaacctt ttttcacgaa agataaagag ttcaagtttt tcaattctgc tttcgtaccg
    64741 aactcttctt gtccataagg gcgaagagaa atattggcat cacagcgcat agagccttct
    64801 tccattttca catcgctgac ttctgtaaat tggataattg atcgcaatgc ttctagataa
    64861 gcgtatgctt cttctggtga acgcatatca gcttcagaaa cgatttcgat caaaggcgtg
    64921 ccttgtcggt tcaaatcaac gtaagaataa ccgccaatac catgcatgtt ttttccagca
    64981 tcttcttcta aatggacacg ttcgatccgg atttttttct ttttaccttc tacttcgatt
    65041 tcgatccatc cgtcatgacc gattggttga tcaaattggg aaatttgata tgcttttgga
    65101 ttatctgggt aaaagtaatt tttccggtca aaatgagtat ctttagagat ttcacagttt
    65161 aatgccaaag ctgctttcat cccgaattcc agcgcagctt tattcattac tggcagcacg
    65221 cctggataac cccagtcgat cacgtttgta ttgctgtttg gttctgcacc aaaatgagct
    65281 ggcgctggag agaaaatttt tgagttggtt tttaactcga cgtggacttc tagtccaatg
    65341 actgtttcaa aattcattcg tctttccctc ctaaaatagc tggttgttgt ttatggaatt
    65401 ctgttgcttg ttcaaacgca tatgccgctt gatacatcgt tttttcatca aaataattac
    65461 cgatgatctg caatccaact ggtaatcctt ctgataaacc tgccggtaca gacatacctg
    65521 gaagtccagc caagttgaca gggatcgtta agatgtcact catgtacatt gtgattggat
    65581 cgttaatgtt ctcaccgatt ccgaatgcaa ctgttggtga agatggcccg atgatcaagt
    65641 cataatctgc aaagactttt tcaaaatctc gtttgatcaa tgtacggact tgtcctgctt
    65701 ttttgaagta agcgtcataa taacccgcgc ttaaggagaa tgtacctaac atgatacggc
    65761 gttttacttc ttcaccgaat ccttctgaac gtgagttgac atatacatca tctaatgatt
    65821 tcacattttc agagcgataa ccgtagcgga taccatcgaa tcgttgcaaa ttggaacttg
    65881 cttctgatga ggcaatgata taataaacag ccacaccgta ttttgaatga ggcaagctga
    65941 cttcttccac aacagcacct aatgaacgga atgtttctgc tgctttcaat acagcttctt
    66001 ttacgccagg ggccacacct tcgcctaagt attctttagg taaagcaatc ttcattcctt
    66061 tgatgtcgcc tgtcagtccc tctgcaaaat caggaactga aacgcctgaa gaagtaccgt
    66121 ctttttcgtc ataaccgctg atagctgtca atgctaatgc attatctttc accgtacggg
    66181 tcattggtcc gatttggtca agagatgaag caaaggcaat caaaccaaaa cgtgagacac
    66241 ggccataagt aggtttcatc cctacgatgc cgttaaaagc tgctggttgg cggatacttc
    66301 ccccagtatc actacctaaa gaaactggaa tttgtccagc tgcaactgct gctgcagaac
    66361 caccagaaga gccaccaggt acttttgttg ggtcccaagc attttttgtt ttcttgaaat
    66421 aagaagtttc tgtacttcca cccatagcaa attcatccat gtttagctta ccaacaggga
    66481 tcatatctgc ttgatagatt ttatccatga ccgttgcatc ataaattggt tcaaaattgt
    66541 aaaggatctt agatgcagcg gttgtcaaga tatcttttgt tacgatatta tccttgatac
    66601 caattggaat accagcaagg acgttgcttt catcaatccc tttttcatct aaagcttttg
    66661 cctgttccaa tgctttttct tcgttcaatg taatgaatgc ctcgacctca ggctctgttt
    66721 ctttgattcg ttcaaatgtt gtttttgtca aatcttgtac agtgatttct ttcgatacaa
    66781 gcatatgatg cagttcttct agagaacgat tgtgtaattc tgtcattatg cgccagcctc
    66841 cccattatcg atgattgcgg gtactttgat aaagccgtct tctgtttccg gcacattttt
    66901 caataattca tcgcggttcc atccagcttg cgcccgatct tgtcgcatca cgtttactgt
    66961 ttcaaccaca ttcgacgtaa atggaacacc ttccgtatct acttcttcca gcatttcaac
    67021 catatcgatg attttaccta attggtcggt aaaacttgca agttcttcat ctgcgaacgc
    67081 aagttttgct aatttggcaa cgtgtttcac ttgttcttca ctaatcgcca tactacccct
    67141 tctttctaaa cgagttaaaa actttgctct ctattttaac acaagagtgc cgtgtctgaa
    67201 attgtttatt gaaatttgtc tgaaaaaact catttttcat tcttctactg agcgactttt
    67261 agccaatgca tctgccattt gatcttctgt ccaaacttcg attcctaatt cttgtgcttt
    67321 ggtcagcttg ctgccagcat cttctcctgc taccacgata tccgtttttt tcgatacgct
    67381 accagtcact ttcccgccaa gattttcgat cgtctctttt gcttcttcac gagtaaatcg
    67441 tgttagttta ccagtaagga ctactgtttt ttctttaaaa ggagactcga cttcttgcaa
    67501 ttgggcattt cgaagccctt tgtattcgaa attgacgcct gcttgtttta attcattcat
    67561 caattcatgg acttcttcgt tactgaaata agtgactaca ctgtcggcaa tgatatcacc
    67621 gatcgtatca agcgcaataa tcgactcata gtcactttta ctcaatgttt ctagatcacc
    67681 gaaatgttct gctaaaattt ttgctgcttt cgctccgaca tgtcggattc ctaacccgaa
    67741 aatcaaccgc tcgaccgaat tatctttgct gttgtcgatt gctgtcaaaa tattatttgc
    67801 tgatttttct tttattttgt ccaacgtcaa taactcttct tctgtcaatt tgtaaagatc
    67861 agcaacatct gctactagtt ttttatcata catctgttct agtacgcgcg gccccaaccc
    67921 atcgatattc atcgcatttc ttgaaacaaa gtggttcaaa ccttctttca tttgagcagg
    67981 gcatttagga ttgatgcacc gtaaggctac ttcttcatcc aaatggacaa gttcacttcc
    68041 acaaactgga caatgtgtag gcaattggta ttcttcgctg tctttaggtc gtttatctaa
    68101 cacgacttgc gaaacttctg ggatgatgtc tcccgcttta taaatcagaa ctgtatcttt
    68161 caagcggata tccttcaact taatataatc accgttatgc aggctggcac ggcttactgt
    68221 cgttcctgct actcggactg gtgtcatgat agctgttggc gttactaccc ctgttcgacc
    68281 gactgtccat tcgatgtttt cgattagcgt ttcaacttct tctggcggaa acttgtaagc
    68341 tgctgcccat ctcggtgctt tgatagtaaa accaagttga tcttgtagcg agaattcatt
    68401 cactttgatg acgattccat cgatttcata gggcaaatcc acacgtttgt catgatattc
    68461 ttcaatgtat gaccacactt catcaatcga atgacataga cgtttttccc gattcgtgtg
    68521 gaatccaatt ttttctagct cttctagcgc atcatactgg gtttttgctt gcataggacc
    68581 gaaatcagct actgtgtata aaaaagtgtc cagatttctt ttcgctgtga ttttagaatc
    68641 taactgacgc aaagaaccag ctgcggcgtt ccgaggattc gcaaagatat cttttccctc
    68701 tgcttctctg tcttggttca attggacaaa tgaccgtttt ggcatgaaac attcgcctct
    68761 tacttcgatg ttcattggtt cttttaattt gattgggacc gaccggacag tttttaggtt
    68821 ttcagtgatg ttttctccta cagttccatc cccgcgagtc gcacctctca cgaaatttcc
    68881 atcttcataa cgcaaagaaa cggaaagtcc atctattttt agttcacagc aatagtcaac
    68941 tacacgtccc actgcttttt gtacacgttg atcaaaagct atcagctctt ctttgctgaa
    69001 aacgtcgttc aaactgtaaa gcggaatatc atgagtgact ttttcaaaac cttctaatac
    69061 ttttccgcct actcgttgag tgggagaatc agacgtgatc aaatcaggat attgttcctc
    69121 gattgctact aattctgcgt attctttatc ataaacatag tcttccactg ttggtttatc
    69181 ttctacgtag tattcgcgag accattggtt cagtcttgta cgaagttctt ctgctcgttt
    69241 agcagcttcg tcaatcgtca tttcatgttc catcatttac cctccactac taagcttttt
    69301 caattggcgc aaaggcagcc aaaagacgtt ttactccttg acttgggaaa gccacatcta
    69361 gttctagatc cttcgacgtc ccgcttactc ggacgactgt tcctagaccc cattttttgt
    69421 gtttgacttt atctccaggt ttccagctgt tattttcccc tccagtagca cttttagagg
    69481 gaacaaccgc tgtctctggc tgtttatagc tatatgctgt ttttttagcg aatacaggcg
    69541 tttttttagg cgtaggacgc atgccttcga tttccagtaa ctcctgatcg atttcttcta
    69601 caaaacgaga aggtcggtta tattgcgtcc gtccatataa tgttctggag aaagcatttg
    69661 ttaaatacaa cgcttcttct gctcgcgtga tccccacata tgcgagacgg cgttcttcct
    69721 ccagttcgct ttcttccatc aatgctcgag ataaaggaaa aaccccctct tctagaccaa
    69781 tcaggaatac aactggaaat tctaatcctt tggctgcatg caaagtcatc aatgtcactt
    69841 gggaagcatc ttcttctaaa ttgtcaatgt cggaaacaag tgccagatca tttaagaaga
    69901 ccgttagttt ttcttctggt gcatcagcgt cttcttcatt ttgctgttcg aattgtttgt
    69961 cgaattcttg tgtgaccgtt aaaaattcct ccaagttttc gatgcgtgct tgtgcttcta
    70021 atgtattttg gatttttaag tcttctagat atccgctacg atccaaaacc tctttcgtca
    70081 attctgtcac tgtcaagtat gggatcatct gagtgacttc ttggatcatc tctccaaatg
    70141 cacctaattg ctgtcctgct tttccaccga tatttgccag atccacattt tgcgctgctt
    70201 caagcagtgg ccattcatga agagaagcaa aactgcgtag tttttcgata gatcctggtc
    70261 cgatgcctcg tttgggcgaa ttaacgatac gctcaaaact gattgaatct tgaggattta
    70321 ccagcacatt caaatacgct aaaatatctt tgatttcttt acgatcgtag aacttgtgcc
    70381 ctccaaccat tttataagga atatttgctt tcaacagagt ttcttccatt acacgagatt
    70441 gtgcgttcgt ccggtacaag atggcaaagt cgccgtaatt ccgatgattg cttcgaatct
    70501 cttcttgcat ccggtctacg ataaaacggg tttcgtctcg ttcattatct gcacggtagt
    70561 aagtgatctt gtttccttct ttgttttctg tccacaagtt ttttggtttt cgattgctat
    70621 tgttttcaat gacttggttt gctgcgctaa ggatgttttt cgtggagcga tagttttgtt
    70681 ctagcaaaat tactgcagca tctggataat ctttttcaaa atccaagatg ttctgcatat
    70741 ctgcaccgcg ccagccgtaa atactttgat ctgcatctcc cacgacgcac aggttcctaa
    70801 atctacctgc caataaattg actagcgtat actgtgcgtg atttgtatct tggtactcat
    70861 ctacatgaat ataatggaat ttgttttgat aatacgtgag tgaatcagga tgttcttcaa
    70921 ataatcgaat cgtattcatg atcaggtcgt caaagtccat acattgatta tattgaagct
    70981 ctttttggta agcagcataa cattttgccg caatctcttc gtaataactg ccttgcattt
    71041 ttgcaaaatc ctgaggggtc tgcaggctgt tttttgcttg agagattgtt cctaatatac
    71101 ttcgaggatc atattttttc ggatcaatgt tcagttcttt tagtatccgt ttcatcaagg
    71161 tcagctgttc agaagaatcg atgattgtaa agttccggtt atacccgata aaatcaacat
    71221 ctcttcttaa aatacgcaca cacatcgagt gaaaagtcga tacccagaca tcttcgcctc
    71281 cagatgcgag aatcgcattg acacgttctt tcatttcttt tgcagcctta ttggtaaaag
    71341 taatcgctag gatgttccaa ggattgactt ctttttcctc aatcaaatag gcaatgcggt
    71401 gtgtcagcac tctcgtcttt ccacttcctg cacctgccat tagcagtaat ggtccgtcag
    71461 tgtgaagaac agcttctttc tgacgggggt tcatcccgtt taataattca gctttggatg
    71521 acattaacaa catcctttct tttatcaatc ttgttaatta tagcattttc ttggctttca
    71581 ttaaactagc tagttcaaaa tcatttgttt ctttttatgg atttttcctt gtcaattcgt
    71641 tgatttcgcg ctaaattaaa agaaagcgag gcgtttgaat ggaaaaaaaa ggaaaaatca
    71701 agaaattaga taagactcag atatatttta ttctaaaagg aatatttgtt ggactgctga
    71761 caggaatcgt cgttagcctg tttcgattat ctatcgaaaa gctaagcgat ttagttcgaa
    71821 gtatctatga aatgagtcgg gaacagccga tttacttaat tggcctagct atttgctgca
    71881 tccttgcagc atttttcgtg ggttatctgg tcaaaaatga accggatatc aagggaagcg
    71941 ggattccaca agtagaggga cagcttcgcg gggaattatc aatgaactgg ttctctgttt
    72001 tatggaaaaa gttcattggc ggtgtacttt cagttggtgc gggccttttt ctaggtagag
    72061 aaggaccttc tatacagctt ggcgcatcca ttggacaagg agctggacaa ttgtttcgga
    72121 gtccatcttc agaagaaaag attttaattt ctagcggtgc aagtagcgga cttgcagcag
    72181 cattcaatgc tcctatcgca ggattactct ttgttttaga agaaatccat cactcctttt
    72241 ctccacttgt ttggctgaca tcatttgctt cagcaatcac tgctaacttc gtctctcttt
    72301 actttttcgg cttgagacct gttttatatc taggtgacct tccttctttg ccgttgaaat
    72361 attatggttc cttagtgatc ttaggagtac ttctaggcat cttaggtttt atttatcaga
    72421 atgtcttgct ggctttgcca aaattttaca accgattgcc tttaccagct tattttcacg
    72481 ggttcgttcc tttcttgctg attttgccaa ttggttattt atggcctagt actctaggcg
    72541 gaggcaatca aatcgtttta gcttttggac atgcttcctt gccaatcatt atgattatcg
    72601 gtttgtttgt tcttcgattc gttttttcca tgatttctta cggtgggggt cttccagggg
    72661 ggattttcct tccgatattg acattggggg ctttaatcgg tagcttttat ggaaacatta
    72721 tggttgattt cgggatggat ccaatctaca tcaaggattt tgtcgttatt gccatggctg
    72781 gttatttcac agcgattgga aaagcaccgc tgacagcaat tattttagtg acagaaatgg
    72841 ttggctctct taatcatctg atgcctttag gattggctgc tcttgttgct tatatcgtca
    72901 atgaccttct cggcggtaat ccaatttacg aatcattatt ggaacgtcta cttggaaata
    72961 ctcagaccgc ctcactaaaa ggtgcaagga caacttttga ttttccagta acggcagaga
    73021 gttctttaga cggattaatg gtacgagact tcaattggcc taaaaatatg ctgctaatct
    73081 ctatacgcag aggaaatcag gaaatcttga ctcacggtga tacgatcatg aatgtaggag
    73141 atattttggt tatcttgact gatgaagcaa acttgccaag aatcaagcag gaaatcgagg
    73201 ctaaatcttc tatcacaaat ttgaagcaat cattttaacg aacatggaaa aactatttta
    73261 caacaactat tcatctacac tacttgattc tatatgattc tataaaaaaa gaagaggctg
    73321 ggaaaaccca gcctcttctt ttttatttct atgcttctgc tactgcagga gcttttctca
    73381 ataaaccata aatcacacca gatacgactg ctccgatagc aataaacacc aaatacatga
    73441 ccgggtgact cagtaagaat acaacgaaaa tcccgccgtg aggagccagt aatttgattc
    73501 cgaaagctcc gactaatccg ccagttaaag cagaaccaat aataaaactt ggaatggcac
    73561 gaatcgggtc agctgctgca aatggaatcg atccttccgt gacaaacgat agtcccatca
    73621 cgatgttcgt caaaccagca tcttgatctg ttttgctgaa tttattctta aacaaacgtg
    73681 tagcaacgaa aattgccaat ggaggtacca ttcctccagc cataacagaa gccatcacga
    73741 cacttccgcc agttgcaaca gtcgtagcta aagttgctgt accaaacaca taagcggctt
    73801 tgttgattgg accgcctaaa tctgccgcca tcattccagc taataatgca ccaagtaaag
    73861 cagcattcga accactcata cttgtcaaga aaccgttcaa tgcatcattg attgctttca
    73921 tagggacatt gaccagcaac atcaagaaac cagtaatcag taagccaaac actggataaa
    73981 agaggatcgt tttaattcca tccaatgatt ttggtaagtt cttgaataat ttacgtaaaa
    74041 atacgatgac ataaccagct aagaatccac caatcagtgc acctaagaat cctgcaccgc
    74101 ctgtattagc taatgcacca gcagcaaatc caacgatcaa accagggcgg tcaccgatac
    74161 ttgaagcaat aaatcctgct aataccggca gcatgaaacc aaatgcagct tgtccgattt
    74221 ggttgaacca gctagcagct tgattgtaat tccctagatt tgataattga tcatgtggca
    74281 cgccaataaa ttggtcgatc atgaaagaaa gcgcaatagc gattcctcca ccaataacga
    74341 aaggtaacat gtgtgatacg ccgttcatca aatctttgta aattctttgt ccgatagtac
    74401 catccgcatt tccttcttct gaactgcttt cttttccatc accatggaat actggtgcag
    74461 taccactaag ggctagattg atcaactctt ctggtttacg gataccatca ctgacaggac
    74521 gattgatcag ttcttttccg tcaaagcgat tcatctctac ttttttgtct gctgcaacga
    74581 ttacaccttc tgcacgggcg atgtcttcag cagtcaatcg gtttttgatt ccttctgaac
    74641 cgttcgtttc gactttgatc tccacaccca tttctttcgc ttttttcttc aaagcatctt
    74701 ctgccatgta agtgtgagcg atccctgttg gacaagctgt aacggcaaca acgaattttc
    74761 tttctggatg agcagaagct tctgcagctt gtgtttcttg ttctgctttt tcttctgctt
    74821 ctttttgctc ttcagctgtt tggaacaagt cttggacttc ttctggtgta tttgcttctt
    74881 ttaattttcc tacgaaatct gcatcaatca gcaatcgaga tagtgctgct aatgcttgca
    74941 aatgtgtgtc gtttgcgcct tctggagctg cgatcatgaa aaacagataa gttggctgtc
    75001 cgtctaaggc ttcataatct actccttttt ggctttttgc gaataaaact gttgcttctt
    75061 taactgcact gtttttcgcg tgaggcatgg cgattccatc accaagacct gtagaagttt
    75121 gtgcttcgcg ggctagaatt ccttctttat atgtttcgat atcggaaatc cggccggcat
    75181 caaacatttt ttggaccatt tcatcgattg cgcctttttt atcagtcgct tgcaaatcca
    75241 tgatcatcgc ttctttgacc attaaatcct tgatattcat gattatcctc cttgaaacta
    75301 atcaattttt gtgatttcaa cttctggcag caactcatca atcaattcgc gagctgccaa
    75361 gtcatcagaa aaagctgttg cgcttccgca agcaactccc catttaaagg cttcgattgg
    75421 atctttcgta cggctgtaag ccccaataaa gccagcaatc attgaatccc ctgcacctac
    75481 tgaatttttc aatgggcgaa ctaatacatt cgttttataa gttccttgtt ccgtgaagag
    75541 caaagcaccg tctccagcca ttgagatcag cacattctgt gccccttcag ctagtaattt
    75601 tttcccatac ggaatgattt cttctacaga ttggaaggtt gtgttgtaaa gctcagctaa
    75661 ttcgtgattg tttggtttga tcaacaacgg tttcttaaca agtgcatttt ttaggtcatc
    75721 acctgttgta tcaatcacga aatctgcacc tttttctcga atgatatcaa tcagttcttg
    75781 ataaaatcct tttcgcaggc tagctggcgc gcttcctgac aatacgacga tatctccttg
    75841 tcctactgtt cctaatgctt gcttcaactc atcgatttct tcatctgaaa tagatgggcc
    75901 taagccattg atctcagttt cagtgtctga ttttagtttg atattgattc ttgtatcttc
    75961 ttttacggtt gtgaacaaag actggatttg ttctttttca agccacttcg aaataaattc
    76021 gccagtgaac cctcctaaaa accctagtgc tgtagattga gaatcgatcc gtttcaaaat
    76081 acgggaaaca ttgatccctt ttcctccagg taacttgaag tcttccttca ttcggttcaa
    76141 atcgcctagt tttaacccat ctacgcgtac gatataatca attgatggat taagtgttac
    76201 tgtgtaaatc attgtattgc ctccttaatt gttgtctttt cacggaattc attaacgaat
    76261 tctaatggac aacgctcggt tatgatcgtg gctgcttcca aaggtgcaac agaggtgaag
    76321 gacaactggt tgaatttggt atgatcaatc aatacaaatg tctcttcgga atttttcatg
    76381 gctaaacgtt taacagctgc ctcttctgga tccggagtcg tgaatccttg ttcaagatgg
    76441 gcaccgttca ttcccataaa tgctttatta aaatgaaaat tttttagttg ttctatcgca
    76501 ttagatccta aaacagcatt agttgccagc tttatcgttc cacctaaaat aatggtagca
    76561 agctccaaat caactaaatt tgccgcatgt tgaacggaat ttgtcactac tgtgatctgt
    76621 tttccttgca gaaaaggaat catttctaac gtagtagagc cagcgtctaa ataaatgaca
    76681 tcttcgtcat taatggtttc agctgccgat ttggcgattt gctgtttttc gtgaacgttt
    76741 ttgagtgatt tttcactcat attctgctca aaattcaata attgctcttt cttagctccg
    76801 ccatgaactc tttctagaaa cccactattc tccagctctt gtaaatcacg tcttatcgtt
    76861 gattcagaag cattcaaaag gtttacaaga tcttgtgatt tcacgatttt ctgctgttca
    76921 agcaagtcta ggattttttg tctgcgttcc tctgtaagca ttttcacgat cacctcaata
    76981 caaataatag catatttttt ttctactagc aatataaatc agtcaaaaac gttcataaaa
    77041 attataaatc agtcaaaaaa aaaataaaaa accagtcaga aagactggtt ttttattttt
    77101 ttgtctatca tcaagccgtt ttatcgtttt ttattttcgc ttcttgtttt tttcatataa
    77161 acagaagcag gttttctttc cttatgaaga agataataat aaagtcccat cccgccaaac
    77221 atgaccaaaa aaccgatgac tgatcgaata ggaaaattag gaacaaaaaa gagacagacg
    77281 gataataatg atgccggaac cattattcga taatagagtt gtttatccat tttcttcacc
    77341 tcatattttt tgtaaagtaa tcaattgttc taattctcgg taatctaaaa attgcacacg
    77401 cagatgctgc tcacctttct tgacatcgaa ttggatactt cgccgctttt tctttattcg
    77461 tttgaaggta acatcagaga gtctatgttt gatttccttt aacggtaatt ctgacaggga
    77521 ttctgaagga acatagattc ttaaggataa aagatactct tgatattttt catctgttac
    77581 ctctacttct agatattccc taatataata acaataagct tcgattccat tatccaaagc
    77641 aacagtcgga tcgataactt tatagcacat catgcgtgat cgattctcgt caacccaagg
    77701 aaaatacgcc tgagcattta gtgttccgcg aacacgcctt tctttataat ctccttgttg
    77761 gatatcataa ctttttgtta caaaaaatga agcattcttt ttaggaacgg gttgcatttg
    77821 tgcattctga tactggacag attggatcgt atggtcagtg taaatgtatt caacgaatgg
    77881 cggtttcaat aaaccgacac cattgtccca agcaagatga tcaaacccaa gctggtccct
    77941 aaaataggat tgaaacattt gattgtttgg cttttgccag ttaaaggggt ctcgataaag
    78001 ctggatacat atgacttcgt tttctaattc cttcactttt tctacttgtt ggatgtcgag
    78061 aaaaatcggt ttagggatga tttgatgata atagcgacta taatacattt cccaacacga
    78121 agtgaaacat aaaccttggt aaaaaagatc gtatcctgga aattggttgc aatcgactac
    78181 tacttccccg ttttgttgtt tcattttagg taaatgttcg atttcatcct ttttctcaaa
    78241 caatagccgt tgagagatct gttttgtatt gtgatagata aactcgctat aagctcgcat
    78301 ataggcgaac accccatgag caatcatttt tttctgaatg aagtctcgaa tcaaaaattg
    78361 cttttctttg aaaacagaga tagacaatac attttttatc aataattgat tatcttccaa
    78421 gcgaaaacta cattcgttct cgtcatttcc tacaactaac caactttcgt ctcttaccga
    78481 tgctagcatt attttttctt gaagctttcg aagatcatac ggatccgctt gttgtgtgaa
    78541 agagcggata aatgttggct ggatcaacag acgtgtcaaa atagcccaat cgcttatttc
    78601 ctgttttttt tcttcttcat ttaagtctgt gagctggata gtcatcaata atttatcttc
    78661 gccaatcatt ttctcacctc tttcaaatct ttttataatt taattataac caatgataaa
    78721 gaaaaactaa aactttatgt cttagaaacc aatttggacg attacatgat agactaagta
    78781 aaaagaaaca aagaggtgtt tacaatgggg ttgaaatttg aacgtacaga taatttagat
    78841 aaacttttcg aatcatttgc tgtcgatccg gaaaaaacag ttgcaccaaa agaagagatc
    78901 gatcgatttc taaaaccaca gccacaaaat gaaaagaaaa aagacaagaa acaaaagtaa
    78961 gttaagctca aaaaa
//
LOCUS       DAFBQH010000005        69008 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.005, whole genome shotgun sequence.
ACCESSION   DAFBQH010000005 DAFBQH010000000
VERSION     DAFBQH010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 69008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.005"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(37..1188)
                     /locus_tag="LK694_000374"
     CDS             complement(37..1188)
                     /locus_tag="LK694_000374"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBK5051274.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1201..1638)
                     /locus_tag="LK694_000375"
     CDS             complement(1201..1638)
                     /locus_tag="LK694_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5051275.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1653..3419)
                     /locus_tag="LK694_000376"
     CDS             complement(1653..3419)
                     /locus_tag="LK694_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBK5051276.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3683..4360
                     /locus_tag="LK694_000377"
     CDS             3683..4360
                     /locus_tag="LK694_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5051277.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4363..5490
                     /locus_tag="LK694_000378"
     CDS             4363..5490
                     /locus_tag="LK694_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5051278.1"
                     /translation="MSLLTFDRNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5690..6130)
                     /locus_tag="LK694_000379"
     CDS             complement(5690..6130)
                     /locus_tag="LK694_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5051279.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6141..8174)
                     /locus_tag="LK694_000380"
     CDS             complement(6141..8174)
                     /locus_tag="LK694_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBK5051280.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8176..9879)
                     /locus_tag="LK694_000381"
     CDS             complement(8176..9879)
                     /locus_tag="LK694_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK5051281.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9973..10923)
                     /gene="manA"
                     /locus_tag="LK694_000382"
     CDS             complement(9973..10923)
                     /gene="manA"
                     /locus_tag="LK694_000382"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBK5051282.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10962..11987)
                     /locus_tag="LK694_000383"
     CDS             complement(10962..11987)
                     /locus_tag="LK694_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBK5051283.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12196..13065)
                     /locus_tag="LK694_000384"
     CDS             complement(12196..13065)
                     /locus_tag="LK694_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBK5051284.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13524..15416)
                     /locus_tag="LK694_000385"
     CDS             complement(13524..15416)
                     /locus_tag="LK694_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5051285.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15413..17140)
                     /locus_tag="LK694_000386"
     CDS             complement(15413..17140)
                     /locus_tag="LK694_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5051286.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17156..17806)
                     /locus_tag="LK694_000387"
     CDS             complement(17156..17806)
                     /locus_tag="LK694_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBK5051287.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18010..18306
                     /locus_tag="LK694_000388"
                     /pseudo
     CDS             18010..18306
                     /locus_tag="LK694_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            18478..19098
                     /locus_tag="LK694_000389"
     CDS             18478..19098
                     /locus_tag="LK694_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBK5051288.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19190..20356)
                     /locus_tag="LK694_000390"
     CDS             complement(19190..20356)
                     /locus_tag="LK694_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK5051289.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20368..20721)
                     /locus_tag="LK694_000391"
     CDS             complement(20368..20721)
                     /locus_tag="LK694_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBK5051290.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20849..21871
                     /locus_tag="LK694_000392"
     CDS             20849..21871
                     /locus_tag="LK694_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBK5051291.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21890..22051
                     /locus_tag="LK694_000393"
     CDS             21890..22051
                     /locus_tag="LK694_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051292.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22310..22744)
                     /locus_tag="LK694_000394"
     CDS             complement(22310..22744)
                     /locus_tag="LK694_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051293.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22854..24713)
                     /locus_tag="LK694_000395"
     CDS             complement(22854..24713)
                     /locus_tag="LK694_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBK5051294.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24831..25991)
                     /locus_tag="LK694_000396"
     CDS             complement(24831..25991)
                     /locus_tag="LK694_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBK5051295.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26298..27722)
                     /gene="araA"
                     /locus_tag="LK694_000397"
     CDS             complement(26298..27722)
                     /gene="araA"
                     /locus_tag="LK694_000397"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBK5051296.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27738..28436)
                     /locus_tag="LK694_000398"
     CDS             complement(27738..28436)
                     /locus_tag="LK694_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBK5051297.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28393..30054)
                     /locus_tag="LK694_000399"
     CDS             complement(28393..30054)
                     /locus_tag="LK694_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBK5051298.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30272..31672
                     /locus_tag="LK694_000400"
     CDS             30272..31672
                     /locus_tag="LK694_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBK5051299.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31924..33003)
                     /locus_tag="LK694_000401"
     CDS             complement(31924..33003)
                     /locus_tag="LK694_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5051300.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33089..34048)
                     /locus_tag="LK694_000402"
     CDS             complement(33089..34048)
                     /locus_tag="LK694_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBK5051301.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34060..35571)
                     /locus_tag="LK694_000403"
     CDS             complement(34060..35571)
                     /locus_tag="LK694_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBK5051302.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35593..36156)
                     /locus_tag="LK694_000404"
     CDS             complement(35593..36156)
                     /locus_tag="LK694_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBK5051303.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36162..37025)
                     /locus_tag="LK694_000405"
     CDS             complement(36162..37025)
                     /locus_tag="LK694_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5051304.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37039..37935)
                     /locus_tag="LK694_000406"
     CDS             complement(37039..37935)
                     /locus_tag="LK694_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5051305.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38110..39399)
                     /locus_tag="LK694_000407"
     CDS             complement(38110..39399)
                     /locus_tag="LK694_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK5051306.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39371..40888)
                     /locus_tag="LK694_000408"
     CDS             complement(39371..40888)
                     /locus_tag="LK694_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBK5051307.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41086..41976
                     /locus_tag="LK694_000409"
     CDS             41086..41976
                     /locus_tag="LK694_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBK5051308.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42049..42873)
                     /gene="nadE"
                     /locus_tag="LK694_000410"
     CDS             complement(42049..42873)
                     /gene="nadE"
                     /locus_tag="LK694_000410"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBK5051309.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42885..44357)
                     /locus_tag="LK694_000411"
     CDS             complement(42885..44357)
                     /locus_tag="LK694_000411"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBK5051310.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44994..45356)
                     /locus_tag="LK694_000412"
     CDS             complement(44994..45356)
                     /locus_tag="LK694_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBK5051311.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45579..46331
                     /locus_tag="LK694_000413"
     CDS             45579..46331
                     /locus_tag="LK694_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK5051312.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46471..47394)
                     /locus_tag="LK694_000414"
     CDS             complement(46471..47394)
                     /locus_tag="LK694_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5051313.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47409..47681)
                     /locus_tag="LK694_000415"
     CDS             complement(47409..47681)
                     /locus_tag="LK694_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051314.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(48151..49776)
                     /gene="groL"
                     /locus_tag="LK694_000416"
     CDS             complement(48151..49776)
                     /gene="groL"
                     /locus_tag="LK694_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBK5051315.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(49827..50111)
                     /gene="groES"
                     /locus_tag="LK694_000417"
     CDS             complement(49827..50111)
                     /gene="groES"
                     /locus_tag="LK694_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBK5051316.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            50336..50998
                     /locus_tag="LK694_000418"
     CDS             50336..50998
                     /locus_tag="LK694_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBK5051317.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(51074..52366)
                     /locus_tag="LK694_000419"
     CDS             complement(51074..52366)
                     /locus_tag="LK694_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5051318.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            52536..53165
                     /locus_tag="LK694_000420"
     CDS             52536..53165
                     /locus_tag="LK694_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBK5051319.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(53268..54077)
                     /locus_tag="LK694_000421"
     CDS             complement(53268..54077)
                     /locus_tag="LK694_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBK5051320.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(54132..55001)
                     /locus_tag="LK694_000422"
     CDS             complement(54132..55001)
                     /locus_tag="LK694_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HBK5051321.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESINAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(55002..56318)
                     /locus_tag="LK694_000423"
     CDS             complement(55002..56318)
                     /locus_tag="LK694_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051322.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(56315..58150)
                     /gene="walK"
                     /locus_tag="LK694_000424"
     CDS             complement(56315..58150)
                     /gene="walK"
                     /locus_tag="LK694_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBK5051323.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(58155..58859)
                     /locus_tag="LK694_000425"
     CDS             complement(58155..58859)
                     /locus_tag="LK694_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5051324.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(59049..59909)
                     /locus_tag="LK694_000426"
     CDS             complement(59049..59909)
                     /locus_tag="LK694_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK5051325.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(59896..60465)
                     /locus_tag="LK694_000427"
     CDS             complement(59896..60465)
                     /locus_tag="LK694_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBK5051326.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            60846..60918
                     /locus_tag="LK694_000428"
     tRNA            60846..60918
                     /locus_tag="LK694_000428"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60879..60881,aa:Lys,seq:ctt)
     gene            complement(61414..61800)
                     /locus_tag="LK694_000429"
     CDS             complement(61414..61800)
                     /locus_tag="LK694_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBK5051327.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(61797..62318)
                     /locus_tag="LK694_000430"
     CDS             complement(61797..62318)
                     /locus_tag="LK694_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBK5051328.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(62320..63354)
                     /gene="sppA"
                     /locus_tag="LK694_000431"
     CDS             complement(62320..63354)
                     /gene="sppA"
                     /locus_tag="LK694_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBK5051329.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(63387..64802)
                     /locus_tag="LK694_000432"
     CDS             complement(63387..64802)
                     /locus_tag="LK694_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBK5051330.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(64771..66924)
                     /locus_tag="LK694_000433"
     CDS             complement(64771..66924)
                     /locus_tag="LK694_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HBK5051331.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            67182..67418
                     /locus_tag="LK694_000434"
     CDS             67182..67418
                     /locus_tag="LK694_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HBK5051332.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            67437..67652
                     /locus_tag="LK694_000435"
     CDS             67437..67652
                     /locus_tag="LK694_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HBK5051333.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(68355..>69008)
                     /locus_tag="LK694_000436"
     CDS             complement(68355..>69008)
                     /locus_tag="LK694_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5051334.1"
                     /translation="KIVLKTQSPLEVKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEV
                     IRKYDVQAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEYRNEIVELLLLAQERI
                     DATKQVTNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCTKDGQYEFYGRISELE
                     KKYPMLTRISRACLVNIFNVKEIDFKKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
BASE COUNT        20185 a        14209 c        11687 g        22927 t
ORIGIN      
        1 aaaatttcgt cacattcccc tttttcgtat cctattttaa gcgacttgct tggcataacg
       61 gttcacgttt tgcttgatat tcccgattgt tttcggatca ttgattcctg tgacttcttg
      121 aatcaattgt tccaagtttt catggatctt catttcatgc aattgacggc tttgttcatc
      181 ttcaggatca tagtaattaa aaataatccc cattgttgcc gtcaaatgtg ggcaagtcaa
      241 gttatgttct tgtagttctc tcactggacg tgtaaatctt tcttgataac ctaacttacg
      301 taatggtgtc cgagcgacac gtgtgatcgc atcagagata tttttgtttt ggaagcgatg
      361 aatgattttt tcaatatacg catgatgctg ctctgcatca aacccccatt tggcaatcag
      421 caaacttcct gtttcctgca aaactgattt taattgtgcc acaactaacg catcttgcat
      481 tgcttcatca atggtctggt aacctaacaa ggcacctgta taagcgacag tcgcatgacc
      541 agtattgaca ctaaatagtt tgcgttcgat atatggttct agatcatcta catataaaac
      601 cccttttaat tggatttttt tattttttct ttggctatcg tcaatgaccc attctttgaa
      661 aggttccact tggacgaata aaggatcttc atggtgttgt aatgggacga ttcgatcgac
      721 tgctgcattt ggaaagccga tatatttgtc agtaaatgat gtttccggaa gatatttata
      781 cacttctttt tctagaaatg ctgaaccgcc aatcatattt tcacaggcaa tgatatctaa
      841 agatgttgtg cttccttcct tttctctttc ttggatacct tttgcaatca actcagcaat
      901 aaaaggtaag atattcgggc caatggcggt agtaactaaa tctgcttcag cgatttcttg
      961 gattactttt tctggatttg tttgattgtt gattcctttg acatttttta cagtgatttt
     1021 ttcttttgat tcatctgcta attcgatttc gtattctttt ctttcattta aagcatcaat
     1081 gatcgtgtga ttcacatcta cgaaagtaat ctcaaatcca ttagctgcca aaacctctcc
     1141 aataaatcct cgaccgatat ttcctgcacc aaaatgtact gctctcattt ttctctctcc
     1201 ttaagcaatt gctagatttt ccgttacttc ttctttgctg agcgcgtctg ctagctggac
     1261 aacattgtcc atatcactgc aataaagtgc gatttgctgt acaagttgca aatgctcatc
     1321 tccgactcct gcaataccga acaaaacagt cgcaatcttt tcttcttctt cggtaccaaa
     1381 atctacacca tctggtactt gaacgacaca gatgccggat tttttcacat atttttttgc
     1441 ttcatctgtt ccatgaggaa tcgcgataaa attccccata taaacagata acattttgtc
     1501 tctatctacc atggcatcga tatattcctc atcaacgcac cctgcttcta caagcatctg
     1561 accgcaatag cggatcgctt cttctttggt tttaaaagat cgattgagct cgatcatgtc
     1621 gaatggcaaa ctttgcatgc tgttttcctc ccttaagctg tctgtttcaa atattgtgca
     1681 atcgattcgt atttttgcgg attgaccatg ctgttgactg ataaatgaat ggattgaggt
     1741 gcagccatct gtactttttc cgctaaactt tctttggcta cgatcagtga atcaggttca
     1801 tcttttaaat cttggatatt gacttttctt aacgggatag ttaaatggtt cttgtctaat
     1861 atatctttga gcacggtcat ccccattgtt gctgatccgg aacgatcatc gtggacaagt
     1921 agaatctctt tgatttgttt gatgtttgct gaaagaaact cttctgctgg ctcattctca
     1981 atttcagaaa ctgatgtttt ctgcttgctg cttaattctt gaatgatttc atcatatcga
     2041 ggtgaattca aaaagttttc tactgagaca aaacgcgcgc tcggtgcttt ttgttttgct
     2101 ctttcttgta gttcgacctg agtaacgatc aatgtattcg agtcatctgt cagttggctg
     2161 attgcttgat tggtcaccgt ttgaggcaag tctgcttcct gtactttttt tctcaagatc
     2221 gaagcgccca tcgcacttga tcccattcct gcatcacaag caaaaatgat tttttgtacg
     2281 ccagagaatg tagctccttg aggaatacct tgggcacctt tggaagcttg cttagctgcc
     2341 tttgtctctt gaacagattt ttcgaagtct tcatttgtat ctttcgcatc cgctttcaaa
     2401 attactgcag aaataccaaa cgaaacagct gcggcaacaa tcacgcccaa aataacagga
     2461 aggtaagaat tgaacggtgt catcgctaga atcgcaatga tcgaccctgg agaagctggc
     2521 gctcttagtc cagcatccat caattggaaa atgaaacttc ctgacattcc tccgcctata
     2581 actgataaga ataataacgg tttcatcata acgtaaggaa aatagatttc atggatcccg
     2641 cctaaaaagt gaatgatcat ggctcctggt gctgaagatt ttgctgctcc tttgccaaac
     2701 aaagtaaaag caagtaatac gccaagtcct ggaccaggat ttgcttcaag caagaataga
     2761 atagattttc cagcctctgc tacttgttca gttcccaatg gtgtcaaaat cccatgattg
     2821 attgcattat tcaaaaataa gatttttgct ggttcaataa aaatacttgt caatggaatc
     2881 aaatgggcag ataaaatgat ctctacacct tttgccattg cttgagtcaa tgcatcgaca
     2941 actggaccaa tcgcagaaaa acctaataac gctaatccaa aaccgataag acctgctgaa
     3001 aaattattga ctagcatttc aaaaccggat ttgatccgtt tttggaatag ctgatcaaat
     3061 ttcttgatgg catatccacc aagaggtccc atgatcatcg cgcctagcat cattggaata
     3121 tctgttccga caatcacacc catcgttgca attgccccaa caactgatcc gcggtcacca
     3181 gcaattactt ttccgccagt ataaccgatc aataaaggaa tcagataagt tagcattggg
     3241 cttaccatgg tagccaaagc ctcatttggt aaataaccat ctgggataaa tagagccgtt
     3301 aatacacccc aggcaatcag tgctccaata tttggcatga ccatgcttga taaagtactg
     3361 cctaattttt gtaccctcgc ttttagcgaa atgttgtttt tgggaatcgt ccgttccatt
     3421 ttgtctctcc ctctctcctt ttgatactct aattgtatcc gcttactaaa aagagaaata
     3481 gtcgtacttt ggcacgaaac tttgtgccaa aggtttgagt atgtcagcaa aataaaaaga
     3541 tttcggtatc agaaaacttc aattttaaat aaaactgtta ttttaatttt tttataatat
     3601 gcaaacaata acttttgtta gcatttaatt tagatgtttt ccattctatt atgttattaa
     3661 attttatatt ggaggcctat ctatgacatt actgatcaaa aacatgaaca tgaaacgtgg
     3721 taaaaaacgt gtgattcgac aattgaatct tttagtagaa tcaggagaag tagctgcttt
     3781 gattgccccg aacggatttg gcaaaacaac tttgctgaat ggcattgccc aactgctgcc
     3841 aacaacttat gatcaattgt cccttcacaa catctctcct actgcttata cagattatcg
     3901 ccggtctttc ttttttctag aatcaacgaa tcagttatcc gactatctat cggctgttga
     3961 ttatttgact ttgatcaaat actattggca gagcaaagta gaatgccaaa ctgttttcca
     4021 acgattgaag atgactgagt accttacttt gccgattgga aaaatgtctc taggaatgaa
     4081 acaacatgtc ttgcttgctg cttacttgat cagtgattca tctgtcatgt tattcgatga
     4141 accgttgaac ggattagacc caggaagtat tgatatcttg aatagcctca tcagacaatg
     4201 gcagaaagag gggaagacga ttcttctttc ttcccacgct atcgcgaata tccaatcgat
     4261 ttgttctaaa gcatgttttc taaaaaatgg agaaatcgtc acagaatcct caaatatgga
     4321 agagatttta gctatttatc gtaaattata tgaggtgtaa ctatgtcttt acttacattt
     4381 gatcgcaatc tcttactaaa aaacaaagta aactttttat ttcctctatt gttggtcgtg
     4441 ctctttgcat tcccactttt ttttgatcat aaacttgctt atacagaatt cgatgaactg
     4501 aatcataact atgaagagat gcaacgtttg attgaaacgt tgaaagaaga tgagaatgag
     4561 aaagaatttg tggaaagttt agagaaaagc aacaaactga tcgaagcaat cttacatgca
     4621 aaaaacacag ggaatgtcca gcaaatggtc gaagcaactt atcattatga aaaagatata
     4681 ctggatcgac tgatttctgg tcaaagacaa ggaattccaa tcattgaaca acaaaaacgt
     4741 gtcgagttgc tccgttatat gaaagaacat cagatccaaa gatattctat ttttgacttg
     4801 ccagcgcatc tccctttagc aaattactac gaaaatattt tttcaggaat gatttctagt
     4861 tttttgattt tatgtataac cgccttgttt ctttcttcca tcatctctta tgaaaaaaga
     4921 aagcaggtaa tctctctagt caatctatta ccggatagta tggtaaagaa acatagcatc
     4981 cgttttacca tttattatgg tgcagctatg ctaagtctcg tgatgccatt tttgatcgtc
     5041 tctatactag taataatcaa aaatggtttg ggagacttcc gttatcctgt aggaacgatc
     5101 attggtcagg agatacgaat ccttccgatg tatgagtatc ttttccagag ctttctgttc
     5161 ctgttgttgt gggtgctctt tttaagtaca atcagttttc ttctatctgc tttatttgaa
     5221 cattctctag tcaatctttt aggtacactt ctttgtcttt tcttagcaga gtatcgcttg
     5281 ttttcttcga ttggatggat agaatcaatc agtcattact tgccaacttc ttatgttgat
     5341 tttcaaaatg tgatcatagg aggagatatt ttctcgccac tagcttcaga acaggttact
     5401 ttcatgaatg gtatcttgac acttggtata tggagtatcg ttttattatt cataggtatg
     5461 ggcactatct atataaaaaa gagctattaa aacacaaaaa taataggggc ggtgacaaaa
     5521 accttgtcac cgtcccctat ttcaaataaa cggttttcta gaataaatag ttttcaaaag
     5581 cttgcatccg cagtaataag aacctcccaa caaaaatagc tcctcttatt tttatcttgg
     5641 ttgaagttat tacttgatgc aaggcagctt tttttactgt gaacattggt tatatatgtc
     5701 catgttcaaa aatcatttgg acttcttcaa tggttttcgc atcactgagc gccattactt
     5761 ggtcgatgtc tgaacagaaa aaggctaaat cttggagcac cgctaactga tgttcttttt
     5821 ttaaagccac tacaaataag agtgtcgcga tttttggttc ctcttttgtt ccaaagtcca
     5881 caccatcagg cacttggact aaatagattc cttcttctac tacttcttca tctttgtcag
     5941 tcccatgtgg caaggctacg aaattcccga tatagacact cgctttttct tgtctttcga
     6001 tcattgattg gatatatgcc ggtttgattc ccccttgttg ggtcatccac tggccgatct
     6061 cggtcaacgc ttctttccaa ttttgatagg attgatcgag caatattttt tctgctggta
     6121 gctgtatcat cttttcacct tcatttcttt atttcttcaa tcaggatttt cgctaacaac
     6181 tgataaacga tttctttatt tcctgagcgg aagatctcgg tgttcaagtc attcatgatg
     6241 attgctccac tgatctttcc taaaagaatc ccgtcttttt cttggattgg cgaaggcgcc
     6301 agcataacta acatacggga caaatcgact ggctgtctat ccatcccttc aatggtcaac
     6361 ggatgtttca aattgaaaat acaaaagatc ggtttagaaa tcaatgagct ggatgtatgg
     6421 aataacccca tatttgtatg aggaataccg ataggtgctt gttcatatcg cttaagcagc
     6481 cggcgatgaa ccacctcttc atccgttact agtccttcag gaaaataagg aaataagctg
     6541 cgaatcgttt cggaaatcgt ctcaggatta tccagttctt ggataaaaaa acgaccgagt
     6601 aattgattga tttgatccat gaaatgcatt gtttcctcat aagtatcttc tatcatccac
     6661 tcgttattgc tgttatacgt aattttttgc ctttttttct tctcaatatg cgaaaaagct
     6721 tcttttagct gcttgacttc atcttctagc agtagcgggg agactaaaat atattttcca
     6781 ccgtatcctg gtaaaataga tgtagaaata acaacatcat attcttcttg taaatcgatt
     6841 ttttttaaat cagcaatccg atagaaatga atttgttgga tgaagggaaa ataccttttc
     6901 aatcgtagtt ccaacatgct agtagaagct aatccacttg gcgaaaatcc tgcaatactg
     6961 atattgcttt ctttcggact tcgttccaat gaattcgcaa aatgaaggac catataagcg
     7021 atctcttctt cggataattt tttttcttga aaaatccgct ctactgcttt acggattgca
     7081 ttcgcaactt cctcgtattg aaccatgatt ttttctaaaa tcggattggt cagtgtctct
     7141 tcttgtaaaa cggctcttga aaacacacca gataaatgag tcaacagcat tttgtacaaa
     7201 gtttcatctt cataaaaatg gacattcgtc tcttcactta cttcttgaat cagttgtttt
     7261 actaaataag caagctgtgt atcgaacgtc tcttcaaaga aattctgatc aaatgagttc
     7321 gtgaagtcat ttaataatcc tgcaaaaaag acgatctcat ttactggata aagttgtttt
     7381 gtctcagaag cgatccgtga aaacaatcgt ttagctgtct gtagtaattc tttgtttgcc
     7441 agactattat atgtctcatt tgtcagtaaa tgatttttag tcacacgctc catcgtcaat
     7501 gtcagcatca aaaccaaatg ttcaagcttt ctatcactta gattggtctt tttttggatc
     7561 aattcttccc cgattacttt gcgtgcaaga gatagatgtt gcggatcaac gaatcgaaaa
     7621 aaataattca gtgatgcatc gaaagaaata ctatgaaaaa gttcatattc attgatttct
     7681 aattctaata cattcgctgt cagaagccgg cggtattttt ccggtccttc gatctcgtat
     7741 ccctggttcc gaatgatttc caaaggcagt tggcgaatac tttcttccaa ctgtttgata
     7801 tctgcataaa aagtggtatt actgatgcga tatgattcca tgaaatgagc aagactcaac
     7861 ggctctgtcg tcagtaacaa ctcggctaaa atcgcgtgtt gtcgtttagc agtggataag
     7921 tcattacttt tcccatctgt attttctttt aattgctcta atacttcttc tgatgcctca
     7981 atgacaaatg agcctctagc tgcttttttc aaagaagcat ttaccgattt caatgagact
     8041 tcaagattat ctaattcgcg gtaaatagtc cgtttactga ccttcaataa attcatcatt
     8101 ttttggatag atacaggtga cgggctactg agaaattctg ctaataattg tttttctctg
     8161 ggcgataaat acatcttata tttctccctt cacgagtttt tcgtattcat tcgtagctac
     8221 ccaatgttca actagaacta gccgagggat cttttcttct agctgttcag ttacagctag
     8281 ttctctttgt gcgatataaa tcgtttcttc ttggattttg attttttcta ttgattcttt
     8341 tgagataaca aactgttttt tcgctttttt tgctaattga cttaaaatcg tcatgcccat
     8401 agtttgtgag ccgcgtctgt tgtttttata caaaaataca atattagcag gcgacttttt
     8461 ttgttctgtg atatctgcta tctctttttt ttcttcccgc tttggaaatt ggatcgcttc
     8521 ttttattcgt gacagcaaga tatctgtttc caaaaagtga tccagtacat agatttgact
     8581 ttgaggtact ttttctcttg caagacccgc tagttcatgt tgcacgataa ccagctgatt
     8641 tacttggtta ttcaattcat agatagagct gtatccgaca gaaagatcga ttttttctgc
     8701 tgctaatttc tttttcagca gggcggctcc catagcactt gatcccatac ctgagtcgca
     8761 agcaaaaatg acggaatgaa tgatagatgg ttccaagatt tctccccttt ctttgaccgt
     8821 tttgttctct ttttctttat ttggtaattt caatagtatc gctgcacaaa tgaaggtcac
     8881 aaatgtactg attgctatcc ctatacttac cccagctagt tgtggataag gcgcattggc
     8941 aaaaatcacg agtagtgacc caggcgatac aggtgcagct aatcccgtat ctaaccattg
     9001 aaatatcaac gtgccgcttg ccccaccagc cattacagct aaaaatagat acggtcgcag
     9061 caagacaaat ggaaaataga tttcgtgaat cccgccaatc gcatggatca tcaatgctcc
     9121 actcgcattt actttttcct gtttttctgc aaaacaaagg atagaaagta agatccctaa
     9181 accaggtcca ggatttgcct ccatcaaaaa taaggaagag tacccgactg tttgtgagaa
     9241 ttcgattcct aaaggtgtca atacaccatg attgattgca ttattgaaaa atagtacttt
     9301 tagaggttct aagatcagac tgacaagtgg taacagattc attgtgacta gccaagacac
     9361 aacggaggcg agcaacaagt gaacactatt taaaactggt tcaataaaaa cgattccaat
     9421 tatacaaata acgctaccag tcagtcctac tagtaggttg ctgacaagca tttcatatcc
     9481 agccttgaat ttcacgtgga atctagaaac aaattgttca tatacgatgc cggcaagcgg
     9541 cccgataatc atcgctccta cgatttgtgg agcattggaa cgagcgatca accctaaaga
     9601 agcaattgca ccggcgactg cccctttttg tccccctacc gatttcccgc ctgaataact
     9661 gatcaagatg ggtaaaagca gctggatcat ccaatggcat acctcttcta atgtggtgtg
     9721 caaatcgcca gacacacctt ttgttgctaa tgacaagatt ccccaagcga taaacagact
     9781 tagatttggc atgatcattt catttaagtg atgtccagct tttctcagtt gtttcataga
     9841 cacgcgactg ctgttgtttg ctttccattt gtattccatc tttttcacca tacttctatt
     9901 tattgatgga cggattttat gtagaaaaag cagcaaacga aaaaccgtct gctgctttca
     9961 gctatctggg aactatttgc ctggttcagt cgcaatgatt gctagttcac cttggaccgc
    10021 ccactcagaa acgccatttg gaataataca gcttgttcct ttttctaatg gatatgtttt
    10081 tccgtcaacg acaaggtctc ctgctccttc gattaccgtc atcaatgtat aaggggcttg
    10141 ttttttgaaa gatgtgattc ctttaatatc ccactcatat acattgaaaa actcagtttc
    10201 taaataagtg acgatcgatg aattcccttt tcggacttct ttgatttgta attctggtgt
    10261 tttcgcaggg acagttgtta catcgatcga ttgttggata tgcagctctc ttgtttgtcc
    10321 ttgcgcatct tttctgtcgt agtcatacac gcggtaagtc gtgtcagaac tttgctgtgt
    10381 ttctaaaatc atgattcctt taccaatagc atggatcgtt ccgcttggta cataaaagaa
    10441 atcacctttt ttaactggta ctttcttcaa aaggtcatcc cagcgcccat ctttgatcat
    10501 ctcagctagt tcttctcttg tctttgcatg atgaccatag atgatttctg caccaggttc
    10561 agcatcgatg atataccagc attcagtttt tcctaattca ccttcatgtt tcatcccata
    10621 ggcatcatct ggatgtactt gtacagatag atcatcttct gcgtctaaga tcttgatcaa
    10681 taatggaaac actggttctg aaggatggcc aaacaattcc ttgtgttctg accatagttc
    10741 atctaatctt ttccctttga attcgccatt ttctactaca ctgacaccat gaggatgcgc
    10801 actgatcgcc caatcttctc cgattttatc gttagggata tcaaaaccaa agactgaacg
    10861 taaacgactt cctccccaaa ttttttcttg aaaaactggt tttaaaaaca taggttcctg
    10921 cataattcaa actcctctca tttttcatag ctattatagc attatttcgg taataccgct
    10981 ttctctttca aaaagttttc aacaatttca tctcgacata tgagatactc cgcacgatga
    11041 tcgacaggat gtacgcgatt tgacaaaaat atcaacgctt cttgtgtttt tatatcaatc
    11101 aagaaaaacg tacctgtata accagtatga aaaagaatcg catgaccgtc ttctacatcc
    11161 ttttttaagt cccatcctaa agaacgatag ccattttttt tcggtgtttg atcttgcagt
    11221 aaagaatcaa tcgtctcttc ttctaaaaaa cgaccattac ggatttttcc cttgttcaaa
    11281 tacatttgga caaaagtaat gctgtctttc aaatcaataa agagacccgc gttacctgca
    11341 tgttcagcca aaactcttgc tttcggatca tgagtttgtc cacgcaaaac ctcacccgaa
    11401 ggcagttgtt ctgtgggaac gatttttttg gaaggaaaag gaagaaataa gctgttcatc
    11461 attcctaatg gttccaatac ttgttcttga aagatttcca ctgcagtttc ttggtaaatt
    11521 tcctctagca tcaacccaag aagaatcgta ccggcatctg tatattttac ttcttttcct
    11581 agttgctcac ctggttctag cttcaaataa gcggcgatca attcttcctg agatagctta
    11641 tctctgtttg gtatccatgt tttgatatca gaagtatgtg tcagtaaatg acgcaaagtg
    11701 attgtctgat tttcaaattc aggaagatat ttatgaagcg gttgatcgag ttctatcttt
    11761 ccatcttcta atagacgtaa aactaatgaa gtggttgcaa cgactttcgt cagtgaggct
    11821 gcatcaaaca acagatcttc tgtcatcagc tgcttttcag gaacgacttg ggcaaagccg
    11881 gctgtaaatg tttctacttg attttctttt atgaaggcga ctgctacacc tggataaaat
    11941 ccttcttcca tctttttcaa aataagctgt ttcgtgtgtg gatacatctt tttctcctgc
    12001 ttttctatca gattcttaca gtccaagctt aaagcttaat ctatgaggaa tcaacctttt
    12061 ttacacaaaa aaactgcgcg aaaaaccgcg cagtttcccc cggtatacta ttttctcttt
    12121 ctatctctag aataagcgat agtatgaccg ttcataaaat gacgagacat gcgacaatcg
    12181 ttaagaaaga aaaagttaag cgtcgcatcg aatgtcagtt ttattctttt cttttggctc
    12241 actgacgaac caccattcga ttcctatagc atcatagatt gtgagtactg tgtgcttttt
    12301 gtctaagaag aaatcaactg atagttgctg caagtgagca atcagtcctt ccatgctttc
    12361 agtatcgatc atgaatttca gaaaatcaag tccaatcact tcatctgtag gcaaatcaat
    12421 tgcgcctccg ctagcttctg tcaaaccaac atggaaatct ccttcattca atacatggat
    12481 gccctcttct tcattatgta ccttcaaacc aagtacatga ttaagaaatt ggcgttcttt
    12541 tgttaagtta gtaacattta aatggatttt ttcaaaataa gaacctgttg atagtttact
    12601 gaatttttct tcagattttc ttaataatgc ttcttgatcc attgtttctg gaatttctct
    12661 ttggtcgttc ttattctctt catggtaaat ttccaattca ttctcttctg ggtctacgag
    12721 caaaatcccc atacgtccat gatcttctaa agcttctttg attggataat tcattttacg
    12781 gatacgacaa aggatatctg ccatttcttc tgtgctaggg ataatcaatg aaaaacggtt
    12841 cagctttttg atcaccccca tgtgatcatt cgctctagga ctttcttcca gcagcaacat
    12901 ttcacttcca gcttcttctg ttcctaaaat ggctaattcg ttttcttctc ttttcaaatc
    12961 aaagccaaca acgttcttgt aaaaatcaat cattttatct cggtctttca cccgaatggc
    13021 tacacttttt aaataggtgg cagcccccaa ttgaaaatct ttcatatttc ttgctcccct
    13081 ctacgtcaag ctttatttta acgatggatt cttcttcttt caagcaataa ctttgatttt
    13141 gttaaaaaaa atgtgagaaa attcgcattt tgcttgtttt ttgtaaatta atgagtaaaa
    13201 actcttataa aaagaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa
    13261 caaaaatagc tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
    13321 gaacaccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat tagagataca
    13381 aaacggaaaa atagcttctc atattttgcc gttttttgag ctaataccga aggacagctg
    13441 attgaacacc gtttattcgg aaaaagggac tgtgacacga cttttgtcac agcccctaaa
    13501 aaaaataata aatttgtact attctatgaa gcttcttctt cagaaaactg actgttataa
    13561 agatctgcat agaatccgtc tttttccatc aatgtatcgt ggtttcctgt ttctacgatc
    13621 gaaccatgat tcatcacaat aatgttgtct gcatcgcgta ttgtagacaa gcggtgagca
    13681 acaacgaaac ttgtccggtt ttccaatagg cggttcatcg ctttttgaat cagtatttct
    13741 gttcgtgtat ctacactaga tgttgcttca tcaaggatca ggacatctgg atttgctaag
    13801 aatgcccgcg caattgtgat tagctgacgc tgcccttgcg aaatattgct tgcatcctcg
    13861 tttaatacag tgttatatcc ttctggcaat ttacgaacaa aatcgtctac gtgagcagct
    13921 ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt tcccatattt gatattgtca
    13981 tagatacttc ctgtaaacag ccaagtatct tgcagtacca ttgaaaactg tgcgcgcaat
    14041 tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac gaatactacc tccagaaaca
    14101 tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc ctgcaccagt cggacctaca
    14161 atagcgacca tctcaccagg tttgacatcc aagttaaaat cagtcattag caagttatct
    14221 ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt atggtgtatc tttttcttct
    14281 ggaaggccag atttttcatt ggacatctct tcttcatcca acacttcaaa tacccgttcg
    14341 gctgaagcaa ctgttgattg gatagtgttc atcaaacttg caagttgagt gattggttga
    14401 gagaactgtg ttgtatattg caagaacgct tgaacatccc ctaatgtcat gttcccattt
    14461 gcaactttta ctccgcctag tacagcgacg aacacatagc ctagattttt aacgaaattc
    14521 atcaacggca tgatgattgc cgaaataaat tgagctttcc aaccagcggc atacagattc
    14581 tgattttctt tttcaaatac ttcctggtct ttttcagtat gattgaatgt tttaacaatg
    14641 gtatgaccac catatgtttc ttctacttgg ttattcaata aacctaaact tttttgctga
    14701 tctgcgaaaa acacttgtga tttcggcgct acgatcatca cgatgatcaa gcttaaaggg
    14761 atcatcaata acgcgactaa cgtcaattgc cagctgattg aaagcatcat cacgatgacc
    14821 ccaatcaatg tgactgcact agttacaaat tgagtcaaat tttgctgcag cgtgcttgcg
    14881 atgttatcca tatcattgat tgctcgagac atgatatccc catttgtatg tgtatcataa
    14941 taggaaatcg gtacacgatt cattttggct tccaaatcat gacgtaattc ataaactgtt
    15001 cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt tgaatacagc agaaatcaaa
    15061 tacatcacga ttactgtcgc aatgatctgc gcgattttat caaagtctat cggaaatttt
    15121 gttacttgtt ggccttgttg catctgttga gcgccagcca ttacaccttt gaatatttct
    15181 gtggttgctt tccccaagac ctttggtgtt tggatttgga agatcacggc tgctattgct
    15241 agtacaaata cagcgatgat cgcaaccata cgtttagaca tatagccaaa caatcggcgt
    15301 accgttcccc agaaattttt aggcttttca ccttttacac cgagatttct gccaggtcca
    15361 ccatgtcctg gaccgcctgc tggtccatga gagggacgtg tgtttgtttt actcatgcta
    15421 agtcctcctc tcttagttgt gaatgaacga tttcttgata agtttcgttt gttgctaata
    15481 gttcttcatg tgttcctttg ccaaccagtt ttccttcatc tagcacaagg atcatatctg
    15541 catccattac tgtacttact cgttgagcaa ctaaaacggt gatactttct ttcatattcg
    15601 ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc caacgctgag aaggaatcat
    15661 caaacacata gatatctgct gttttgacta atgcccgtgc aattgccaga cgctgtcttt
    15721 gtccaccaga gaagtttcct cctccttgtt ccacatggct atctaagcca tcttccatct
    15781 ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt ccaaatcatt tcatctgttg
    15841 catccggctt accatactgc atattgtctc ggatcgttcc agagaacaga actgcttttt
    15901 gaggaacaaa gcccattaat tctcgcaaat tatattgagt catgtcacta atgtccgttc
    15961 catttatttg gatcgaacca gattcgatgt catataatcg agggatcaaa ttgactaagg
    16021 tcgttttacc tgaaccagtc ccccctatga ttgcgacgat ctcgcccgac ttggcttgga
    16081 aatcaatatc ttccaatgca agtttttctg ctccatgata tcgataattg acatgattga
    16141 aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt aggattttca gggtcatgga
    16201 taccgatttt ttcatccaac acttcattaa tacggtcagc tgaagcttgt gcacgaggca
    16261 ccatgataaa aatagcggaa agcatcataa agctgatcaa gatctgcatc gcataagtca
    16321 taaatgcaat cagattaccg acttccaaag tcatatctgc tatgtagtgt ccaccaaacc
    16381 aagtgattgc tacgtttgta ccgctcatga tcaatgtcat cattggcatc atcaatgcga
    16441 cgatcgtatt aactttgatt gccgtatttg tataatcttt attcgctaaa tcaaaacgat
    16501 tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct gacccctgtc aatccttctc
    16561 gaaataccag gtttaaacgg tcagttttct tttgcatact tttgaataat ggaacagcaa
    16621 agtacatgat tccgccgatc aaaataatca ttactgggat gacaactaag aagatttttg
    16681 tcaactgatg atctttttga taagcaagta tgctcgcgcc aatcaacgtg attggtgcat
    16741 tgatcatcat acgtaaaaac atttgtgtaa ccatttggat ttgagtcaca tcattggtcg
    16801 ttcttgtgat caaagaggcc gttccatatt tatcaaacgc atcttttgtc agactctctg
    16861 attttttata gacatccgta cgcaattgct tacccagttt ctgtgattca cgagtagcaa
    16921 aaaatgtatt tgcgatagca gctaagatac tgatcaaaga aaagccgatc atcacaaaac
    16981 ctgtatgcca aatataatcg acatctcctt gcgctacccc tttatcaata atattggagg
    17041 taagtgtcgg caaatataaa tctgccagta cttggatcac catgaaaact gcagctgcaa
    17101 ttgctgaagt caaggagatc cgttttacga tttttagcat ctagttccct tctttctatt
    17161 tatatactcc gtttttcagc caagaaagtt tcaaattgaa ttcactgatt gcttgctcta
    17221 ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg cgcaattgtt gtaaatacag
    17281 tattcatcac taattggatc agtaattcca attcatgatc attttttact ttcagcttcg
    17341 tccgatcgat caattcaagc aactcttttg catcttgttt gtgttcttta tgtggatcgt
    17401 gaggcgtttt ttggcgactc gtatgaggag cgacttttct tgaggcacgg taatccatgt
    17461 tcataaacaa gttacgataa aaagaagcat tctcgccttc caaaatctca taaatcattt
    17521 tagaaaaata ggattcgaat cctttgaata gatcaccatc ggattctttc attagttgta
    17581 acatatcttt agagctgtca cgtctcattg tctcaaagta ataaaaataa agatcctctt
    17641 tatcttcaaa atactgatag aaactgcctc tagggatatc cgctaatttg acgatattag
    17701 caatcgaagc ttcttttaag ggtactctag aaaactcgat tctcgcagcc tcgattagtc
    17761 ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt cggcatatgg atcctccttt
    17821 ctttcaagat gacacattgt catatgacgc tgtgtcatta taacacctcc tacctgtttt
    17881 gcaagacatt tgcatagttt ttttcattat catccgatac attaaaaaag tgataagagg
    17941 aatccttcca tttgaattaa tgagagttgc ccattaaaat agccacatac taaacaaagg
    18001 atgagataaa tggcccaatc aattagtgtc caagacttca ataaattgcc tctcgatgct
    18061 gacagcatcg ttttagatac aagaaatacc ttagattaca caaaaaacca cttaagccac
    18121 tcgctttcta tccccgctgc tatcttgcca agacaattaa aaaatctaga tccaaagaaa
    18181 ctttaatata ttctaagcta tacaggcagg cgatcagaag cattagccga tcaacttact
    18241 gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg aagctcttca gtcgagtgtt
    18301 gcttgaatat tgcttgttag attcaaaaga ggctgtgaca aaaaatcgtg tcacagcctc
    18361 ttttataccc tgtctttttt tagttattta ttcttcaacc ttatcaatat gacttgatag
    18421 ccttttcttt ttctcatagt tataacaatc atagcaataa aaggagaagg taagcatatg
    18481 gcatctgttt tagtagtcaa aggtcatccc ctaactgcag aagaatcgcg cacagttaag
    18541 gcattgacgt cttttttaac aagttataaa gaaaatcatc cagatgatga agtgactgtt
    18601 ttggaacttt atcgcgatga tattccggaa atcgatgaag aattattatc aggttgggaa
    18661 gcattacgtg ctggtgcaga attcacttcc ttatcagaaa gtcaacaaca aaaaattgct
    18721 cgtttcaatg aattgactga tcagttcctt gctgctgata aaatcgttat cgcaaacgcc
    18781 ttatggaatc taaatatccc aacaaaatta aaagcttggt tcgataccgt caatgtagct
    18841 ggaaaaacgt tccgctatac agaaaacggg ccagaaggat tagtgacagg taaaaaagca
    18901 ctgcacatcc aatctaatgg cggtgtatac aacggacaag actttgcctc tcaatatgta
    18961 aaaggtattt taaactttgt aggcatcgat caagtagatc agctattcat cgaaggaatc
    19021 gattatgacc ctgaccgtgc agatgagttg atgcaaaatg cattagataa agctgctgct
    19081 ttaggtaaat cattttaaaa attgagataa atgcatacaa aagagacaga ggaaaaaatc
    19141 ttcaaagcag catttgtacc ttggatattt tcccatctgt ctctttttgt tattctttct
    19201 gctttttcaa cggattttct tcataaagag aagagattgt tgtgtaccat tcaaaaacat
    19261 gagtaatcac tactttagct gctgcataaa caggaatccc caagatcaca ccgatgattc
    19321 cgaacaattt cccagaagtc agcagaacta ataaaatcgt tactggatgg attgctaact
    19381 gattccctag tactaatgga gagatcaagc gtccttctat tgtctgctct actgcaaaaa
    19441 cgatcagtac tttgatgatc atgaatggac ctgctacaat ggctaaaaag atagccggaa
    19501 tcatcgctaa aaatgatcct aagtaaggga tcaggttcaa aaaacccgca ataatcccca
    19561 acgtgacagc atactctaaa ccaatgaccg caaaaccaat cataaacatg attgccacag
    19621 caaaagccac agttagttgt cctcttatat aagaagaaac ttgactattc atttcactca
    19681 gcacattcaa cgtcggtttt tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta
    19741 attgtttacc gtctttcaat agataaaata agataaacgg catcgtcagc agagccacta
    19801 aaatagtcgc taccgcgccg acaaaacttc caagactctg aactgtactc ttagatatat
    19861 cttggatcat atcgcctgcc gaattcatga acttatcacc tgctgtttcc aactgttcgc
    19921 gaaattggtt gaacagcggg tcccgcaaaa tctcggttaa tttattatca atcgtatcta
    19981 catattgagg gaagttactg atgaagctca ctgtttgttc ctgaatctta gggatgatca
    20041 ctacgctccc ccatgcgatt agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa
    20101 gccttggtac ttttcgcttt tcaaagtaat caactaccgg attcatcaag taatacaaga
    20161 ttcctgctaa aatgataggc agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca
    20221 aatatgaaac ttttgtaaat aaaaagacga tcagcaatat taacaataca acaagtaagg
    20281 ctgtgactac ttgattattt aaaaaccagc gccaaaacca cgataccggt ctgttcttct
    20341 tattttttcc ttccataatt cctcctgtta gttataaaaa atttggcttc ccaaaccaat
    20401 agcaggcagt ctatgcggat gaagatagat tccatttgca atttgtcctt cttctggaca
    20461 agcatcaaaa atcaatgata cgtgagctac tgtctctaaa ttcccattgg ccatcggacc
    20521 gacataatcg atcgtatata cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga
    20581 aaaaggttcg tcttttgata cttcttgaat caccgaatat tttcgtaagg cagctgttgc
    20641 tgttttatcg aacaaaatca gcatgggttc ttccttgttt attgcttctg tcccaatttc
    20701 tgtaatcttt gcctgaatca ttgcactctc tcctttaatt gctttcaaaa acagtataac
    20761 acattctcat tctttagccg tgtggaataa ttatgtgata gaataaaaac gaaaactatt
    20821 tcattacaat taggaggaat cagctcatat gatcgaaaag ataattttag gaacctatac
    20881 gcgtcgcgaa agtaaaggaa tctatacgat cgaccttgat acagaaaaag aagaattagc
    20941 caacctttca ttagctgttg aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg
    21001 caatctttac acagttacta gtgtggatgg attaggtggt gccggtgcgt acgataagga
    21061 ttttcatttt ttgaatgcgg taacagagtc tggcgctcct ttgtgttatg tagcggttga
    21121 tgaagcacgt caattactat acggggcgaa ctatcataaa ggtgaagtaa atgtctacca
    21181 tatattagga gatggcgaac tgaaagcagc agattcaata ttccatcaag aagcgacagg
    21241 tcctcataaa aaccaagatc atgcgcatgt ccactatacg gatctgactc ctgaccaacg
    21301 attagtcgta tgtgatcttg gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg
    21361 caaattgaat cttgtaacta ccttcactgc agagccagga acaggccctc gccatcttgt
    21421 ctttcatcca aatggacaat tcgcttattt atttggtgaa ttagacagca cagtccgcgt
    21481 gctttcttat aataaagaaa caggtgcttt tgaagaatta cagaaaatat cgaccttgcc
    21541 agaagaattc gacggtgaaa atggaggggc agctatccgc atttcaaatg atggcaagtt
    21601 cctttatgct tctaaccgag gacataactc gattgctgta ttcgcagttt ttgaagatgg
    21661 ttcccacatt caaaacaagc aaatcatctc aacagaaggg gattttcccc gcgatttcgc
    21721 actagatcca agcaataatt ttgtcgtgtg tgccaatcag aacaccgata atctgacatt
    21781 atatcgccgt catcctgaaa caggattact ggaattactg aaaaaagatg tctatgtacc
    21841 agaatgtgtc tgtgtttatt ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca
    21901 agacgtatta gacagactac gggaagaact aaatattcct ttctttgatg ggaaaatcga
    21961 agataaagag tatagcgaag aggaatatca gaaactcaaa gccgatttac agaactactt
    22021 tgaacaatat gtacagaatg tagaaaacta atcagctttc catactatct gaaaagaaca
    22081 tcaggacaag acttttccgt ctatcctgat gttcttttct acaaataaaa aaccgccgag
    22141 atggttagac tcgacggaaa aggagtattt actcgatttt gagtaagtac aaggttgttg
    22201 ttggttatag gtatgtcatt atcataaagg attttgatca ctctatcata aaggtaatat
    22261 tacatttata ttagataact ctagttttta ttgttatcaa agatttcttt tattttttca
    22321 acaacaaatt agtcattttt tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt
    22381 ttgcttcaga tgtattgaat agatcttcat ctacccctga ttcaagatcg atactttgga
    22441 tcatttcttc acagcctgct acatatgaaa gataagcttt ttcaaaattt ttatgaatgc
    22501 ccattgcttt tgctggtgga cgcaaagtac tgactttctt aagcattgcc cgatattttt
    22561 ttgttccttc ttcaaataac aggatgatct cttcaagagt cgatttgtcc agttcacttg
    22621 tttttccttc atcaattgcg tttctgatca cttcatattt cggatgcatt ccttctccga
    22681 ttgcttctgt ttccttgacg atttgatcga tggactgcac ataatagcca atattcggta
    22741 acatgttcgt tttcctactt tctccattgt tgttattatt tattctactt gtttttatcc
    22801 tcaaatgaca agtacttgaa aaaatttaat ggtttctata cagctcgcca ttcttattcg
    22861 tgctcaaaga aaccatctgc taaactatat tgtttccctg cgacaatgtc atattggatc
    22921 aattgatatt cgtcgtaaat ctcttgttgc gagcgattga attgcgcttc tttataccac
    22981 tgtccgtttt gatagtacag ttctcgttca aacgccggaa gttcctgttc taatgataaa
    23041 agcagctgat aaaagccatt tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa
    23101 taaaacggac tggtgattgc atcgtgagtc gtttgctttt ctaattctcc tttactgtcg
    23161 aacataagaa attgtgtttc atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct
    23221 gaatagatcc ctggaagatg atccccccaa aaaacaacta aagtccttct agaaagattc
    23281 ttcaattctt ccgtgaactg cttcagtgcc gtgctgctat aggaaatatc ttgcagataa
    23341 ttctctaagt caagtgtccc acttccatct gaagtttttg caggatagtc tagattatca
    23401 tactttccat cgtagggcat atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt
    23461 ttttcttcct tgagaagtgt cattacttct ttgtaagctg actcatcaga aatatatgga
    23521 ttattctcta tcgtatccgt ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct
    23581 aatgtctgat aaacatcctc tcttttatac atcgaagtat tgtatggatg gatcgctgtc
    23641 gtctggtatg attgtgcgtt taacgctgaa acgatagaag gaagctgatc catttttggt
    23701 acaagcatcg tgtaaggggt ggtgagttgc gcattaaata acgccataga aaaaccagtc
    23761 aatgcttcaa attcgatatt tgctgttcca ccgccatagt tttgagaaag catattgcca
    23821 ctatatgttt gatcagctac ttcataataa tctgccaatg gctcaccaga tacttctacg
    23881 ccgtttaatc gactaggatc tgagaaactt tcactcatca cgaacacgat gttcggcgac
    23941 tcttcctcta cagttttatt tttttcatcg gctagcttct ggtatttttc agtgatttct
    24001 ttgatctttt ctttcgaata tccttcaggt tcatccattg gttctacctt cagattgtac
    24061 aaaaacccac cgataaaacc agtgttatag tagttcattt tctggctgta aggtatccat
    24121 agtgccgttt tattatatgc ttttcgcaac aaattatcgg gattgttgaa attactgata
    24181 taccctagca gtccaattgc cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt
    24241 ccttttttca ggcttcgaaa catgtaccag caaaacaatc ctatcacggt tcctgcggca
    24301 gctaagatca ctgtaaacag catagtgccg gtcatatctt tcagcaatcc gatttcagtg
    24361 atcatcttca aatcgtctgg ataaatcggt tcttgccgat aactcatttt catataattt
    24421 gcaaaaccta atacaccgat ggctacgaca tacgtaaggg aacctaaagg gacagatcct
    24481 gctagtgcaa tcagaaaaat aagaataatc agcaagacga gacaggctaa gaagaatttt
    24541 tccgtatgcc aagaaaaagc aaatttcaac gcaaggtcta cggatagatc attttgacac
    24601 cactgcaaaa ataaattact aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt
    24661 ccttttaaaa tctttttctg tttggtcgaa agtctgctta tatatctttt caaatgcttg
    24721 ctccttcatt tctatcaata aaagaatgtt gcttctctta ttttagttgt acgaaattcg
    24781 aaagtaaaga acggaaagaa gaattaatga aatccaaata ttctctttct ttatggcctt
    24841 tctggtttct ttaataagcg gacctttcgt actccagaaa ttttttctag ctgttgtttc
    24901 acttgattga gctgttcaaa atcagtactg tctatgtcaa tcaatatgta gacatgctca
    24961 ttcttatggt tacgcgatat atctgcgatc gctagctcgt tctcattcag cagagaaaat
    25021 atctctgcta atatattggt tttcttttga taaaatatag tgaatcgaaa cggcgcttgg
    25081 aaaaacattc gtgcggatgg ataattgacc gcttctctaa ccgtaccgaa caaaagataa
    25141 tcttttaagg cttcaactgc caaacgtccg ctgtctgcca aagcttcctg agtcgatccc
    25201 cctagatgag gcaacataag tatacggtca ttttctaaga actcttcttc tggaaaatca
    25261 gtcaagtatt tagccagatg ttgtttttcc aatgcttgca taacagcata tttatcaaca
    25321 atttcacttc ttcctacatt gataagtaca gcatcttttt tcattttttc tatattcgcc
    25381 tgatcgatca atccttttgt atcttctgtc aacggcagca tgactacgat gtaatccgag
    25441 gtagaaagaa ctgtttctaa atctgcttgc cgaacataat ccaactgggc atcgcggata
    25501 gaataaccca agacatccat ccctaagcta tagcaactta gcgctacttt tgtgcctatc
    25561 gctccaagac caagaagacc gatggttttg ccttgcaatt ctcgtcccac ataagcactt
    25621 cttttgtttt ctgcctgttc taaaatatta ggacctgtta gtgtttgtac catacgactt
    25681 gcttcgatta tcggacggga agataagagc aaacagcaca aaacaagctc ttttactgca
    25741 ttagcattta cacctggtgt attcatgacg atcgtcccgt tttcagaagc tttttcaacg
    25801 ttgatcgtgt tcaccccaac accagctctt gaaattgcta gcaaatctgg tgtaagccat
    25861 tcatctttca ctttcgtact acgaataact attccttcgg ctggcaaatc atctattatt
    25921 ttgaaatatt ccccttgtaa cttttctatc tcttgttctg gaatagcatc aatcaaatga
    25981 acgtgataca taaaaccccc tccttactaa caagtatctc gaaatttctg caattagaca
    26041 actgtttacc atttgacttc ctagaagcaa aaagagcagt tagagcagag taggctgagt
    26101 gataaaaata aggctatgac aagttactgc cataagcctt attttcgaga gcaaaatatt
    26161 gagggtgaga tatcttgata aatggtgttc cgttagctga cattgataat aagactaaat
    26221 ttcaaaaaag ttgaaaagca aatttcgtca atctcttctt atttcttaaa actaagtggc
    26281 tttttcacaa cctcttttta tctaataacc actgtttcca actcaactaa ttttgcccac
    26341 gtttcaattt gatctgttgt tagatttagc gagacaacgg tatgatgacc gccacctgcc
    26401 tgaatccatg aatggacacc ttcatggaaa ttcggtttag ttttccataa cacacgtgca
    26461 acaggtaaat taggtgcttc agtcgttggt tcaaaggctt ctacttcatt gatcagcagt
    26521 ttataatgcg taccgaaatc tgccatcgat acgactaccc cttctcctgc tttcccatca
    26581 aatacaagac gcgccggatc ttctctatct cccattgata aaggagaaac aacgattctt
    26641 ggtttcgtcg cagcaagcgt cggatctact tccatcatat gggattccaa gattgcttct
    26701 tggccactag ctagctcgta agtataatct tccatgaatc ctgtatcttt ccctcgagcc
    26761 atgattttca gcaaccgatc aatcgctgca gttttccagt cgccttcccc agcaaaaccg
    26821 tatccttctg ccatcaaacg ttgtacagct agtccaggta gttgtttcat tccatacagg
    26881 tcttcaaaat tagtcgtaaa agcgttatag cctccttctt ctaagaaacg gcgaataccg
    26941 atttcctgct gagcttgtac ttttacatgt tcctcccatg tcttttcttc ataatcacca
    27001 tagtcgaagg tatacaattc cttgtactct tcaaataatt gatcgacttc ttcgtctgat
    27061 acgcgatcga ttacttgtac aagatctccg ataccaaaat aatcaacagt ccaaccaaat
    27121 tgaatctgtg cttccacttt gtctccttct gtgacaccca cgtttctcat gttgtcacca
    27181 aaacgagcca cttttattcc aaaactttca ttataggcta ctgcaacatc catccaatca
    27241 gcaatctctt tttggatctc aggtcgtttc cagtagccaa cgacgatttt attttgttta
    27301 tttaatcgag cattgatgaa cccatattcg cggtcaccat gagcggattg atttaaattc
    27361 atgaaatcca tatcgatcgt tttccaagga atgctttcgt tatattgcgt agctaaatga
    27421 agcaatggtt tttgtagaag ctttgtcccg cggatccaca tttttgctgg tgagaaagta
    27481 tgcatccaag tgatcacacc tgctacttct tctcgataat tgacctcttt catgattttt
    27541 gtgatcgtat cagcagtaac tgctaattct tgaaggcgga ttggataagg caattggcca
    27601 ttttcattca gttcatcgac catttcttgt gcatgtttct ttacttctcg cagtgcttct
    27661 tccccatata aatgctgtga tcctacaaca aaccaaaatt ctttttctcc aatattcaac
    27721 ataattagcc actcctttta tttgttttgt ccgtaatacg cattttttcc gtgttttcgt
    27781 aaataatgtt tatctaaaat ccgttgaggc agctcttctg aaaatgaatt gagctgtctg
    27841 gtaaataaat tcatcttgca tacttcatct agtacgactg cgttcattac cgcactttgc
    27901 gcatctttcc cccaagtaaa tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag
    27961 ggatcaattt tgcgttcttt gaatgtttct atgataactt ttcctgtttc atattcgtac
    28021 ccgctgtcga tttcctgttg tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta
    28081 tctgcatgcg tcgtccccat cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc
    28141 catgtcgagt gagtatggac gattcctccg atttgaggaa atgaacgata taaaacagca
    28201 tgagtaggcg tatccgaaga gggattgagt ttaccttcaa tgatatgatt gtccaagtcc
    28261 acaacgacca tgtcgtctgc tgtcatctct tcataactga ctccgctagg tttgataacg
    28321 aaatatcgtg tttcaggatc aaaagcgctc acatttcccc atgtatattt gacgagtccg
    28381 tgtttaggca agtcaagatt tgcttggtat acttcttctt tcagttgttc cagcattttc
    28441 tatcactcct ttttcttgca attgatgaat cgctgttttt tcaatgtcga tcccctcacg
    28501 gtaacgtctc ataaatatct gatagcctgc tatttcttct gctgaaggtg tgatggtgat
    28561 tccttggtct tgtccaaaca cgtcttctgc caagaagttt tctaaagact tcccatgtcg
    28621 atccatcata taggctgcta ataacgcgat tccccacgct ccaccttctc cggctgtgtc
    28681 catcactgta actggtgcct ccatagcaga tgctagaatc cgctgtccaa cttctggtgt
    28741 tttaaaaatc cccccatgcc ccactaaacg atcaatttgg acattctcag atttcaatat
    28801 atccattccg attctcattg caccaaaagc actggctaaa tggatccgca tgaaattcgc
    28861 taaatcaaaa cggctacctg gtgtacgtac aaataatggc cttccttcat ttactcctgt
    28921 aatgttttca ccggaaaaat aaccataaga aagtaatccc ccgcaatccg tatcgccttc
    28981 tagcgctttg ttgaataata ccgtgaataa tttatctctg cttatctcca tacccaaaga
    29041 attggtaaat tcttcaaaaa tactgaccca agcattgata tccgaagagc agttattcgt
    29101 atgaaccatg cctactagtt ctcctgaagg tgtagtgacc atgtcgattt ccggatggac
    29161 attttctagt tctttttcta atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt
    29221 ccctgtccgt ttggctacac tgttcgtagc gaccataccg gttcctgcat ccccttctgg
    29281 cggacaaacc gggattcctg gtctcaaatt tcccgtaggg tcgatcagat aagcccctga
    29341 ttcgcttaac ttgccagctt cttctcctgc tactagaact ttaggcaaaa tgtgatccaa
    29401 gtcaatcgct acgtgcgctt tttcgattaa ctgaccaaaa atatttttca tttcttgatg
    29461 gtaggttttc gttgaaggat cgataggaaa catgcctgat gcatctccta cgcctagtac
    29521 tttctcacca gtaagctgcc aatggacata acctgcaagc gttgtcaaaa aattaatttc
    29581 tttcacatgt tcttctttat tcaaaataga ttgatacaaa tgagcgatac tccatctctg
    29641 cggaatattg tattgaaatt tttccgttaa tattcgttct gcttcttcag tgattgtatt
    29701 tcgccaagta cgaaatggta ctagtaactg ttccttgcta tcaaatgcta gataaccgtg
    29761 catcattgca ctgaacccaa tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa
    29821 tacttctgct gccatttttc ggtaactgac gcgcagtcct ttccaaatat cttctaatga
    29881 ataggtccag ataccatttt ctagtttgtt ttcccactca taactaccag cggcaatagg
    29941 agtaaaagat gaatcaatca atacagcttt gatcctagtt gatccaaact caatccctaa
    30001 agatgtacga ccatctatga catctaacat actttgcgca tttacttcct tcataatgat
    30061 ttaactccct ccttctcgtc aaacctgatc aaaccgtctt tttagtagcg cttacaatga
    30121 ttattgtata cttttgtacg tacatgtcaa gcggttattt acaagaatac aataaaaaac
    30181 acttgttttg tacgtacata agttataaaa tgaaattgct tacaaaatct gatcaaccgc
    30241 cttctcgaaa ttctagaagg agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc
    30301 gtttatttat ttttttggcg catttggcgg cattctattt ggatatgata ttggtgttat
    30361 gaccggcgcc ttaccttttt tacagcatga ttggggtttg gcaggtaaag cttctttgat
    30421 aggatggatc acgtcttcgg tcatgttagg tgcgatcctt ggcggctctt tatctggtct
    30481 tctttctgat aagcttggac gacgaaaaat gattcttctt tctgcactta tttttatggc
    30541 tggttctgtc ctttctgcat ctgcccctca caatggttct tattttctga ttgcagcaag
    30601 gattcttcta ggtttagctg ttggcgctgc atctgcccta gttcctgctt atatgtctga
    30661 aatggctcct gctagactaa ggggaagatt gtcagggatc aatcaagtga tgattgcttc
    30721 tggcatgctc ctttcctatg ttgcagatta tttgctaaaa gtgttgcctg aaacgatggc
    30781 ttggcgcgtg atgttagggc tcgctgctgt ccccgcgctg attttatttt tcggtgtttt
    30841 agctctacca gagtcaccgc gttttcttat gcaatctggc cgcttggaag aggcaaaaag
    30901 agtattgaac tatattcgga caccaaaaga agctgaacag gaattcgaac aaatccagct
    30961 gaatgtcaaa caagaaaaga caaccggaac ttcttggcat actttgtttt tagaaaaata
    31021 tcgttcctta gtcattgccg gtattggtgt agcagtattc cagcaatttc aaggagctaa
    31081 tgctattttt tactatattc cgctgatcgt agaaaaagcg actgggcatg cagcgagcga
    31141 tgcattgatg tggccgatca tccaaggaat cattttagtt gctggttctt tgctcttttt
    31201 ggttattgca gacaagttca atcggcgaac tttattgaaa ataggcggta gcgtgatggg
    31261 actttcattc atcctaccag ctgttttagg aactgtactg gatgctcata caaattcgtt
    31321 gttgattctc ttgtttttat gtatttatgt cgcattttat tcttgcacat gggctccact
    31381 gacatgggta atcataggcg aaatattccc actagctgtc cgcgggcgag catctggcct
    31441 agcctcttct tttaattgga ttggttcttt cctagttggc ttgctttttc ctgtgatgac
    31501 tgcttctatg tctcaagaaa tcgttttcgg catttttggg atcatctgct ttttaggggt
    31561 tttatttatt caagagatcg taccagaaac tcgtgggaaa tctttagagg aaatcgaaca
    31621 aagtgcatct aaaaaaactt atcctaaacg tatttctatc catcattctt aaagaggttg
    31681 tgacagaagt ggtcagcttc aagaaataag aaggaaccgc ttagtttcca gtcacaagga
    31741 acaatttcaa aaaacagctt ctcatgtttg atgaatattg tgacttgtga ccagttggta
    31801 ggagaacacc gtttagtgga gaggtggtga aagaaacgtt cagccttgag cgatgacaaa
    31861 agctctcaca aacattgcga cgagtaaccg taggagcaat aagaggcggt aaaaacccgt
    31921 ctcttatttt tcgttttctt tcactgtttc ccgttcaact agtacaggtt catagagaat
    31981 atttggctgt gcctgtcctt tttcaatcgt tgtgacgatc caccgtgcgg catcttgtcc
    32041 caacttttct tttggatgcg tcaatgttgt caatttttta ttccctaaat ggcttaaaga
    32101 agaatcgtct gttcctacga tcgataggtc atctggaata tgatagccat tttctgtcaa
    32161 cttatctagc attaaactag ctatttgatc gttatagcaa atgatgccat ctaaagcttg
    32221 ttcttctttc aaccgttgta ctgttttttc agcgattgtt tctcgtgttt ccgtggtgaa
    32281 agtaatgata tcttccgtca aaaaacgcac attatactgt tcacacgcgc tgataaaacc
    32341 tttcatcctg tattttcctt gaagatcgtc gatttttgtg atgaacaaca gacgctgatg
    32401 tccttttttt atcaaatatt ctgctgccat aaaaccgact ttcacatcat ctaaacaaat
    32461 ataagaggca tttaactctt cataagcagc attgatcatc accatcggaa tgccttgttc
    32521 tctcaaagca acataaagtg ctaagtttgg gttatactga ttacttttcg taggctcaac
    32581 gatcaaacca tctaccccat aagcaatcat tttcttcaaa cactctttct ccaattcgtg
    32641 atcgttattc gtactggcta aaagcaatga atacccagat tctcgtaatt cattttcgat
    32701 tcctcggata atcgacggaa aaatataatc agagagatat gtggtgatga caccgatcgt
    32761 ttttgttctg ttcaatggtg ttttttcttt cttatagctg tcatctacat atgtgcccga
    32821 ccctttttct ttacgcagat agccctcatt taccaacaca gcgattgctt gccgaatcgt
    32881 atgtctgctg acgtcatata tttcttgcag cttgaattct gagggaatgg ttgtatttac
    32941 ttcgtattcc tcacgaagga tcttttgttt tatctcttct gcaatatctt gatattttgc
    33001 catttgcctt gccccttttt atttgtacgt acatatatca taacgagtct gcaaggttta
    33061 ttcaagcaaa aatcaaggtt tcttgtgctc ataaggcata cggaacaggt tcaccaaatt
    33121 ctggaaatcc ttgctcattc cacgtaaagc attgcgcgtt cgcgtgccga ttaggattgt
    33181 ctaacggatc ccctttttgg ttcttctctg gtcgagcatg atagatcaaa atatcaaagc
    33241 acccgtcttc tgaaaccgta aacgagttgt gaccaggacc aaattgctta tttttttcag
    33301 aagaaacgaa tactggctcc gataatttat gccatgattc ttttaataaa agattcttgt
    33361 gctcatcagc ccacagcagg cccatggcat agttttcatc tgttgcacta ccagaatacg
    33421 taatgaatat tttcccattg cgagtaagga ctgctggacc ttcatttact gaaaaacccc
    33481 gtttttccca ttcatactca ggtatagaaa ggaggatctg cttccccttt aacgtccaag
    33541 gattatccat ttcggataga taaagattag aattcccagt aatttcagga tcttgttggg
    33601 cccacacata atatagcttt ccttgagaag agaaagttgt tccgtctaaa ctgaaagttt
    33661 cccttttcgt ttttatttgt cccttttcta tccaatctgt attcattgga tctatatgct
    33721 gattttcaag tacgaacatg cggtgatgat gagattcatc acgtatagtt ttatgattgc
    33781 ttgcagcaaa gtaaatatac cattttccat tgatataatg gacttcaggt gcccagatca
    33841 attcactcat cggtccttct tcatgagcct gccatacaat caggcttgca gcatgttcta
    33901 attcattcag tgactttgcc cttcttaatt caatcacttg gtagcctgga accgatcctg
    33961 taaaataata ataaccatct gtatgtttat atacccaagg atctgcccgt tgccgaacga
    34021 taggattatt ataaagtgtc ttcatcataa caaatttcct cacattttct ttattctaat
    34081 tacattccaa gagtattttt ctatccctac atttaattcg tcatgttttt ctattgaaaa
    34141 atagtttatt tcaggtttta ttttttctgg ttcttcacgt gtatttttgt ccaacagatc
    34201 gctactggtt aatgtgtagt gctctccttg atctagcaaa ttgaaatcat gttcaatcgt
    34261 tatcttttgt tgttcatttg tgcgattgat caagaaaata gtcagctgat tgccgttatc
    34321 tacgatcaca ctgtccacat aaggaacatt ttttatcttt tcacaatcat aaacagggca
    34381 aatgatttgt tgtttcaata cttgaccttt agcatatttc gataagtgtt gcatcacata
    34441 ataagttgga ttcctccatg cttgtccttt agcatctgtc agtattaaag gtattgtgtt
    34501 tactaacaat gattgacaac taatctttac gcgatctgcg tgtcgtaaaa tagccagccc
    34561 taatgaacca aataataatg tatcttccat agtaaagtaa caccaatcga tgggactacc
    34621 gacttcaaaa tccctatgtc ttttttccgg ttgggcatgt acattccatt catcaaacgc
    34681 tagatacatt gttttctcac ttctcaaata tccttttaca taatcacaag tagcaacgat
    34741 ttcttcaatt tgacgatcga atcgataagc ttttgcaagg tatgtcgctg tatcatcacg
    34801 ctcattttta ggaggtttag tcaagtcttc ttcatcatac ttatcaatat agttatgcaa
    34861 agctaaataa tctacattct cataagcttc ttctaatgcg atacgatccc attcagggta
    34921 cgatggtaaa cgtggcgtag agcttccaac taagattgtt tcaattgaat catccactaa
    34981 cttcatgact tttgaggctt catttgctaa cctaccatat tcataagcac tcttagcccc
    35041 cacttgccat ggaccgtcta gctcattccc taaacaccat aatttaacgc caaaaggctt
    35101 tttttgccca ttttttattc ttaaatcact ccagaaggtt ccttttggaa aattacaata
    35161 ttctactaaa ttttttgctt catcaattcc ttttgttcct aaattcaccg tgaatatcgg
    35221 tttcgcttgt acatgactga tccatttaaa aaattcgtgg agtccaaaag aatttggttc
    35281 tatacctcgc catgctaaat caattttttt tggtcgttgt tctttcctac ctacagtatc
    35341 cacccaatca taaccagatg taaagtttcc tccaggatag cgaatgacac ctaattctaa
    35401 ttcctttaca taagagagga catcttgtct aaacccgtca ccatcagctg tttcatggcc
    35461 cggttcataa atccctgtat agacgacact tcccatatgc tcaacaaatg agccaaacag
    35521 ccgagaactg atctctccta ccgtaaaatc ctctctgatt tttactttca cttcttcgtc
    35581 caacagcttt ccctaccttt catttcctat agtcaagata atagatacaa gtttccatac
    35641 gccaccagca aagataaaaa taaaaggagg gaaaagaaat atgagcagta caaatcctaa
    35701 taacaatacg caacaaagaa tatttctcaa aaaatcaaaa aatactttcc atgcactttg
    35761 ttcaaaaatt tgtaaaaatg tgatatcttt gttttcagaa atcacatata tccaggataa
    35821 aagaaacatg gaataaataa gtaaaactac atatccacta attgaaagta cgattgtctg
    35881 ttcttctaat aattgattaa caagaaaata aataatgatc gttaacagac taaggaacaa
    35941 gttcttacca aaatgttgct ttattccttg aaagaaaaaa taaatgatat ttcctgatcc
    36001 ctcacctttc cattgcgcca ttacataaaa aacagcacat gtagcaggca cgatagtaat
    36061 aacaggcaaa cacacgatta cccagagaat acctaataag atgtaatcag aaaccacaat
    36121 caactgtcca taaaatttag tctcaactat tttcatcttc ctcactcttt cattccagac
    36181 atagctgttc cttgaacaaa atatttctgt gcaaaaatat aaaccactag taatggcagc
    36241 attgaaataa ccgttccagc cattacaggt ccataaaaag aaatgttctg ccctgtaaat
    36301 aaagctaacc ctgctggtaa agttctcatt tttgaactac ttgtcaatat caatggataa
    36361 agcaagtcat tccaactatt catcagcgtg aaaatcccta atgataaaag agtaggtttg
    36421 ctcaatggca acatgatttt caaaaaaata ttaaactcac ttaatccatc aatgcgtgct
    36481 gcctcttcca aagaatctgg taaagtaata aaaaaggaac gcatcatgaa gataccaaat
    36541 gcagttgtca tacgaggaat gatcaaacct gcataagtat caagcatgcc aagcaaattg
    36601 acttcaataa atagaggaat catgaagact tggaatggga tcatcattgt cagcaaaacg
    36661 aaatagaaca aaactgattt tcctttgaaa cgcatacgag caaatgcata ccccgcaaga
    36721 gaatcaaaga atacagaagt gatcactgtc cctccagcaa aaatcactgt atttttgatg
    36781 taatctaata aagggatcgt tgtccaaacc ttaatatagt tatctagggt atagctatct
    36841 gcaaatattc taggcggaaa tgagataatg tctttttcgt gtttaaaaga agaaataatc
    36901 agccacaaaa aaggaaacgc aataaccaac aatataacta gcatactaat cgttcgaatg
    36961 atttttttac cactaactgc ttttcgacta ttcacagtaa ctgtaatcga gctttctggt
    37021 gccatacagg tttccctcct atttcatttg tttttctttc cgtgacatgt acacattagt
    37081 ggagatagct atagttgcaa caatgataaa caaaaccgaa gacagtgctg atgcatagcc
    37141 caattcataa ggtgctgtaa atcctcgact gtaaatatat tgaacagctg tttctgtctt
    37201 aaattgtggg ccaccagcgg ttgcaacata tacttggtca aacacttgca tcgaaccaat
    37261 cagattcgtt aaaatacaaa aacctaagga gggaacgatc tgaggtagcg taatgttaaa
    37321 aaactgctgt cgatttgttg caccatccat ttgagcagat tcatacaaag agggagagat
    37381 tccctgaata gcagtcaaga gaatcgtcat agtaacacca aaattcttcc aaacagtcat
    37441 gatggctacc gtcggcatag caagggttgg atcgcgaaaa aattgaggga tcggcaagcc
    37501 gacttgtgaa aacaagtaag gaatcgcccc tacattcgga tcaagcaaca tagaaaaaat
    37561 aatcgcaatt gacgtcaatg aacaaataac tggaatgtaa aatgtcgaac gacaaaaacg
    37621 attgaatctg gtatttttag ataatgctac tgctgctaat aaaccaacga taatttgtgt
    37681 tggcgtttcg agtacggtga aatataaaga atgaatcaac gaattaactg ctcgttcatc
    37741 actaaatata cgtgtgaaat tttcaaaact tacaaaattt cttgatgtaa agaaaatatt
    37801 caaatctgta aaactaatag caaatgttcc taaaagcggt atgacagtaa ataataataa
    37861 aactaaaaaa gatggagtta aaaacaaata cgcagcacga tcaggacgat tccaatattt
    37921 ggtgaatccg ttcatatagt ccacctcatc tttcattttg ataaaatata caatcttggt
    37981 ttaaacgaat ctgaataaga tatccgaaca atcgctgtga tttttgcaat aacacctatc
    38041 tgcaaatact aggtcgatca ttcagatatc tgtatcttgc caacagtcta tcctgtataa
    38101 agttctgtac tatttttgca gtaattgatt gatggcatca tctgcatcgt tcatgagtgt
    38161 ctcaggatct tctcctgcta ataatttttc aatcagtgga ttgatgatat cgttattgat
    38221 cgcagagatt tgggtaaaac ctggtaaata tggctctgca tagtccattt gtttcgataa
    38281 ttcagataca ataggatcat tttttacttc ttcatctttt gctactgaat ccaagtaggc
    38341 aggaaaacca ttttcgatac tccatttttt accaatttca gttgtattcc aatatttcaa
    38401 aaactcatag acagcttctt ttttattatt atctgtactt gccggaatgg catatcctac
    38461 gccatctaaa atagctgttt ttttgtctga atcactccct tgtggtaagg tagtcacatt
    38521 ataatcaatc tcgtttgctt tcaatccgtt atttaaccat ggcccattga tttcaatggc
    38581 tagttgacca gcattcatta aattgtccat ttcggcgcca gaaatcgatt ctggaccagt
    38641 cttgtctaca tggatcattt tttggatcgc ttttaacgtt ttaaggttct caggagacgc
    38701 aaattcagac ttagtataat catcgttcac taatttaccg ccatttgcta acatccagtt
    38761 atataacggt gcagttccat ccttattgaa gacaaaacct gatacatttt tactactatc
    38821 agttaatttt ggtgccattt ctgtcaattc ttcccaagta gtaggtggtt gttcagtatc
    38881 taatccagct tgtttaaaca aatcattatt ccaatacata tacatgcctt gtacctgcat
    38941 ggggacaaaa tactgtttac cttctacttc acctaattca atagccgttt ctttaaaatc
    39001 agatttatct actccatcat attcccagaa atcgtctaat ggttgtaccg ctccgttttc
    39061 tacatactgt gcaaaatctg gataatttaa tgcaataaag tcaggtgctt ttttagaaga
    39121 aatcgctgta ggaagttttt cattcaaatt ggcccaagtc atgatatcca tttcaatttt
    39181 gatgttttta tcatttgttt tattaaattc attaacgatt tcttgcaata tttcaccatc
    39241 agatgcagta aacccattcc aaaaagtcaa agtcacttct ttatcagaat cagattctgc
    39301 tgaacttccg ccaccgcaag ctcctaaaat acctgttgca gctaccaaca aagagccaat
    39361 taaccatttt ttacctttca ttttttgttc ctccttcatc tgataaacga catctgacca
    39421 cattccaaga tagcggtttc aataaacaat tgattttccc atcttcagct tttacatttg
    39481 ttgaatgact tggttttact tgttcacttt taggaagatt agttgctttt gtatcaaatc
    39541 cactcatttc agaaaaatca atcactccct ctaatgataa ttcagacaag tgaacagtta
    39601 gattcatttc ttcttctcca cggttaactg caaatatgac aagttctttg tcatttatta
    39661 cggctatact ttctaaatac ggcacttctt caaaatcttc cgtcgcatag tttgctgaac
    39721 gaatttgtgg gcttaacacc tctccttgtc catacacagc agcttgcata aatggataat
    39781 agatcgtctg tctccaagct tctccgtttg tttctgtcct gattggtgca ataacgttta
    39841 ccaattgagc aagacaagca atctttactc tatctgcatg tttcaataaa gtgattaata
    39901 gacagcctaa tagtaatgca tcttcaaaat tatagttatc ttctagtaaa ggaggagcaa
    39961 tctgccaagg cgctgcttgc tcatcttgtt ccgctgaatg ataccaaata ttccattcat
    40021 caaaggacag gttgatctgc ttcttactat gtttcttcgc cttgactgta tcgcacatcg
    40081 ctgtcacttc agaaatgaac cgatccatgt ctaaggaacg tgcaagataa tgagaaagat
    40141 catttttttg gttgccataa tattgatgca gagacaaata atctatttga tcgtacgctt
    40201 gatcaagaac tgttaactcc caatcaccaa atgtaggcat ttgccgattc gaacttccgc
    40261 ataaaaccac ctcaattgaa tcatctacga gtttcatcgc cttagccgtt tcctctgcta
    40321 accgtccata ttcttccgct gttttatgtc caatctgcca aggaccgtcc atttcattcc
    40381 ctaaacacca tgtcttaatt ccaaaagggc tctcttgacc attcgaacgc cttaaatcac
    40441 tccaataagt tcctttagga aaattacaat attcgactaa attacgagct gcatctactc
    40501 cacgagtacc cagattgaca gccatattga tttcggcccc cacatgctga caccagttat
    40561 aaaattcatg aatcccaact tcattcgttt caattgtccg ccaagctaaa tccaaccttc
    40621 taggtctatt tgctatgggc ccaatactgt cttcccaacg aaatcctgac acaaaatttc
    40681 caccaggata tctaatcatg ggaatgttca attcttttac caagtgaatg acgtcttggc
    40741 gaaatccact tttattagct gatggatgat ctggctgata aatcccttca tatactgctc
    40801 gtcccatatg ttcaataaat gaactgtaca accgcggatc aactttgcct acacgcttga
    40861 cactactaat caacattgtg gcttccataa ctgacctcct ttaatttata catgaataat
    40921 acacctcgtt gaaagcgttg tcaataatat aaatgttaaa cttacttatt ttttatcgat
    40981 tttaattaac caaaatatta ataatgatta aaacaaataa tttaatataa cttttattaa
    41041 caaatatgtt aatatataag aaaaaccttt taagaggtga tatatatgca actacaaata
    41101 aacgaagaat ccttaccggt atatgaagca ttagctagta aaacaaggat caagatcatc
    41161 cagctacttt ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact tggagtgagc
    41221 agtgctatca cgacgatgca cgtgaaaaag ctagaggaag caaacatcat caaaacagaa
    41281 aaagtaggac aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat tagttttcca
    41341 gaaaaaatat tcaatgcttt tgatacgaaa gaaacatcga tacctattgg tcactataca
    41401 aattatgcaa ttgaaccaac atgcggttta gctacgatcc atgattttat cgggaaagtg
    41461 gatgaaccac gctattttat ggatcctcga aggatggatg cccgcattct ctggtttacc
    41521 agcggatttg tcgagtatca ggcacctaat tttttaaata cagaggatac attggagatg
    41581 ttggaagttt ctgtggaaat ttcttctgaa tttccttttt caaatgacaa ttggccttca
    41641 gatatcactt tcagcctaaa tggtgtggag ctaggaacat ggacgagccc tggagatttt
    41701 gctgatatcc gcggtaaata tacgcctgat tggtatccag ataatcttaa tcaatacggc
    41761 ttgctgaaga cgatccgaat taccaaacac ttgacgaata tgaatggaga acccttgtca
    41821 aacatcacga tcaacgacat ccctaaagag caagacactt ggcatttgcg aatcgaagtg
    41881 aaagacgatg ccaaacatgt ggggggctgt actttatttg gtaaaggatt cggtaattac
    41941 gatcaggata tcaaattaaa agtttactac agctgatatt tttcttttct attttataaa
    42001 acaaaaacag ccaatcccta tgaagggacg gctgtttttg ctttattgtt atttccagaa
    42061 atcatccaaa acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc accatgcttc
    42121 gattttttct tgtgcttgag cgggaacttc ttttccttct agataatcat caatttcatt
    42181 atatgtgaca cctagtgcga cttcatctgc aatcattggt ttcccatctt caagatctgc
    42241 agtcggaatt tttgtgtata atttttccgg tgcattcaat gcttgaagca attgtttccc
    42301 ttgtcgtttg tccaaacgga ataatggcaa aatgtcggct ccaccatcac caaatttcgt
    42361 gaagaatcca gtaatgtttt ctgcagcatg atctgtccct aatacggctc ctgctctttc
    42421 tccagctacg gcatattgcg taatcatacg ttgacgtgct ttgatatttc ctttgttaaa
    42481 atcgctgatt cccacacctg cttcgcttaa tacttgtact tgtgcatcaa cagctggttt
    42541 gatatttact cgcagactaa catcgggctg gataaagtca agagcagctt ttgcatcttc
    42601 ttcatctgct tgttcaccat aaggaagacg aacagcgata aattgatagt tttcatcttt
    42661 tgtttcttca cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta aagtagaatc
    42721 ttggccgccg ctgataccta gtacaaaagt tttaagaaac ggatattttt tcaaatatgc
    42781 tttcataaag tcaatacttt tacgtatttc ttcttttgga tcaattgttg gtttgacacc
    42841 taattcattg ataatttctg tctgcaggct catttattct cctcctatgc ttctgtatga
    42901 agttctgcta cgcttttttt cattcgttcc atgatagcca ttttatggtt ccagcattca
    42961 gtagacagat cgactgggta tttttgcgga ttcaagtctc gtttatactc ttcccatagt
    43021 gaatctagat tttcttttgt atattctttg atttcgttta gtgcaggtaa ttcgtaaact
    43081 ctttttcctt cgataaagat atcttgtaaa actgggcgag ctgtaaagtc tcgaacagtt
    43141 ttattgataa acgtatgcac tggatggaac atgaagatag cttcttcttc tcgagggtct
    43201 tcatcccata atgtcacgta gtctccttca gatttcccat caaaatttcg agtgatgcgc
    43261 cacacttgtt ttttccctgg agtagttact ttttctgcat tgcttgataa cttgatcgta
    43321 tccatcattt ttccttcatc atcttcgatc gatactagct tgaatactgc accaagggct
    43381 ggctgatcat aggctgtgat caattttgtc ccaacacccc atacatcaat tttagctttt
    43441 tgcatcttta aattcaaaat cgttgcttca tccaagtcat tcgaagcata gatttttgct
    43501 tccgtaaatc cggctgcatc taattgttcc cgaacacgct tagatatata ggccatatct
    43561 ccactgtcga tgcgtacgcc ttggaagttg attttgtcac ctaattcttt cgccacgcga
    43621 atcgcacttg gcacacctga tttcaacgta tcataagtgt cgactaagaa cacgcagtct
    43681 ttatgggttt tagcgtaggc cataaatgct tcataatcat ttccataaga ttggactaac
    43741 gaatgagcat gggtcccgct agctggtatc ccaaatatct tcccagcacg tacattgctt
    43801 gttgcatctg ctccgccgat atatgccgct ctagttcccc agactgctgc atccagttct
    43861 tgtgcccgtc tagttccaaa ttccaatagt ggatcttctc cgatgacaga tttgatacga
    43921 gctgcttttg tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc tgtttctact
    43981 aattggcagt gcgctaaagg cccttccact tggatcaatg gttcatttgc aaaaactagt
    44041 tctccttcac gagcagaacg aactgttgct ttaaattcaa aattttgtaa atactccaaa
    44101 aaaccttcag gatagacttc taatccacgc aagtaagcaa tatcggaatc attgaacgtc
    44161 agattctcta aataattgac taaccgttcc aatcctgcaa aaaccgcgta tccgtggtta
    44221 aaaggcattt ctcgaaagta acattcaaaa acagcatgta aatcagctcg acctaattcc
    44281 cagtatgtct gcatcatatt gatttgatat agatccgtat gcagcgtcaa actgtcgtct
    44341 gggtaagtat tattcatctt gtattactcc tcttggcaag ttttcatttc ctacccatta
    44401 taacaaaaaa aattaatagg aaaagaaatc ttccattttt tctgaaaaag cacatgtttt
    44461 acggatattt cttgctaaaa atgaactcca tctgctaaaa ctatcctttc ttattggtat
    44521 ttaacctaaa cgagaaagaa tacttatttt attcaatgta tccgagacac gaagaaatta
    44581 gcgttttcta tttttcttac ttgataaaat aagtagattt tcctactaca atcgtagccg
    44641 ttaagatacc gatgattgaa caaatataac caaatgtctc tacaacataa tcaatggcaa
    44701 aagtttttga agcaataata cgtctttcct tttttgcttc ttatccccaa agaaacggat
    44761 ataacttatt atgtattgaa tttttttgga aaggcgaatt atgacaagat ataccttgcg
    44821 ttgaaaaaac tcgcggaaaa aatatcacac gtttttaaaa agatacaaag taagaaagca
    44881 gctgtcaata gaatggaaac aaaaatggcg agacatcaat cagtctcgcc ttgtttttta
    44941 ttgtatacat tagatatgga gcaggttacc ataaagatga ttattagtcg ttattaaata
    45001 gaatccttcg tatttttttg ttctatcact acaatatctt tatttggacg tttgaattgt
    45061 attccggcac gttctagctc ctgtttcact cctacatgga ctttgacatc ttctcgtgtc
    45121 atttttaaaa cgtgatgaat cgcatagtcg atttcttgcc ttgtgatttt tttcttcgta
    45181 ttgatcatca cgacttgctt tttatcatat gtgtctacca atagcagttg tgttcgatct
    45241 aacgaaaata atttgtaata attaatagcc gtattcccaa aaaagaattc cactacttta
    45301 ggataaaatg tcttaatatt taaattacta ttcacaggtg tttcgattag cttcatctca
    45361 ttcctctttt ctatatagtc gatttagatc caatcttaag ttatgttacc atctttccta
    45421 gcattaagga atcggttaca attgaacctt taaagacaaa aggatttacc ttttatcact
    45481 taatagtata acataatttc atgagaaaaa aatacaattt tttcttgacc atctcgttac
    45541 gtaaaggatt atgcttacat ttgtcaggag gaaaaataat ggagtacaca ataaagaaaa
    45601 tgtcagagat atcaggagtc agtccgagaa ccttgcgctt ttatgatgag attggactgc
    45661 taaaaccagc taggatcaat tcttcaggtt atcggattta tggcaaaaaa gaagtcgatc
    45721 gtctgcaaca catcttattt tatcgaacaa tggcattcaa gttggatgat atccaagagg
    45781 tcttagacaa tccatctttt gaccaccaga aagctttaat caagcatcag caaatgttgc
    45841 tggaaaaacg ggcacaaatc gataccctct tgacaactgt ccaacagaca cttgatatgt
    45901 acgaaggagg aagaaaaatg agtgacaaag aaaaatttga agggtttaaa caacaaaagt
    45961 tgaaagaaaa tgaagaatcc tatggacaag agattcgaca aaaatacgga aaaaaaaagg
    46021 ttacagcatc caatgaaaaa tggatgaata tggataaaga aacgtatgaa gaaatgcaaa
    46081 ctgttgaaaa gcagatgtta tttgatctca ctctttactt aaaagaacca gaagatgaaa
    46141 gcttagcaga ccgtatcttc caagctcata agaagtggtt atcttacagc tggcctgaat
    46201 atcagccaaa agcgcataaa ggactaggaa tgatgtacga agccgatgaa cggttcactg
    46261 catattatga cgagcgaagt ggtcatggtg cagcaaaagc tttaaatgaa attatccaaa
    46321 aaaaggcgta atcttttaca tgatccaaca aaaaagtaca cgtaaaatga cctaataaca
    46381 aaacagcaga cacccaagtt agatttttat gctaactttt ggtgtctgct ccattctttc
    46441 acaagttttt tcatcctact ttaaatatat tcatcgttga ataaacgtat aaggtatttt
    46501 cactttatcc tttttttctg taagaaacaa ttgaaatgcc atttctccgc aagcttccag
    46561 atggtggtcg atggttggga aaccaagaag ctcgctgatc agcaaattat cgcgtccaat
    46621 caccactggt gtcttatctt gcagatagct ttgcaagatg cctgctgcaa ccatatccga
    46681 atttgtaaag acaccatcaa ctccaagctc tttaaaaaag aagcctgctt gttttccttt
    46741 ctctgcatct atacaatccc aaaaaacaag tgattcatca aaagatggaa aaagttgatg
    46801 acatagctgc aaagtgattt ttgagttata acttagcctg ccacttcgcc ccaacgtgat
    46861 gcccaattgc tttatacctt gctgcttcaa ataagtaaga ccttctgtaa ttgattgttc
    46921 tcgatcaata aagacacagc ttgcctgttt tcccttgatt tcttcacaaa aaactagagg
    46981 tccatatttt tgatatgcca ataaatcatc tagtgtattg gcacgagacg taatgatcaa
    47041 tccatcgaaa gctttagctg caaattcttc aagatacctt cgctcttttt ctttctgata
    47101 gcccgtcggt aaaagtgtca ctttgtaatc ataatcaaac gctgcttcaa tgatcccact
    47161 gatcaactga tcaaaatacg gatgattggt ataaggcaga atcactccaa tgcttttcgt
    47221 ttttccataa cttaactgtc tggcatttgc attagggata taatcgactt ctttcatgac
    47281 cgcaagtatt tctttccttt tttcctcatc tacatatgga tgtccattga ttacacgtga
    47341 aatagtagaa actgaataac ctgtgagctt tgcgatatct cgaatgtttg ccactgctgc
    47401 ttcgccacct atacaaaaag attttcttgt aaatagtcta ccacagctgg accgcattca
    47461 aaaaactgat acatttttcc ttcatgcgaa aaactgatcc gctgcccgcc ccaatatata
    47521 tcttttgtca aattttcgat ttcattcatt ggtatttctt ttactatatt cctattcaac
    47581 ccttgaaaac tgtacaacgt taatacggat cccttgaact cgacaaacgc ataaaaataa
    47641 ttgttcgttg tgccatctgt tatctctaca taagtattca ttgcaagccc tccttttgat
    47701 actttgagta tagtgtatga aacgcgtctc atagcaagga gtttgttttg ccaaagaaaa
    47761 gttttattcc tctttcatta ttaccaaagt ctcttatttt aaattgaata cattataatg
    47821 atactgtaaa tatatcttta ttattagtga atggatattt acttttaata gataaaatat
    47881 tataactatt aaaaataact attttcatta taattacaca caaaaaagag cgtgagacaa
    47941 aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacagcggaa gcagaagcaa
    48001 ctccttcgga aataagctga aattcacaaa aatttgaaaa gcaatttccg tgaatttctt
    48061 cttatttctt ggagttaaac gcttctgttc cagcctcttt ttatctgtct ttcactaatg
    48121 atcagttgta gataagcaag ttgctttttc ttacatcata ccgcccatca ttgatggatc
    48181 cattgcagga gctgctggcg ctgctggttc tggtttatct gcaacgactg cttctgtcgt
    48241 taataacaag gcagaaacgg aagcggcatt ttgcaaggca gaacgtgtta ctttagttgg
    48301 gtctacgata cctgcttcaa ccatgtttac ccattctcca gtagcagcat tgaaaccagt
    48361 gcctagttcc acgttcttca atttatcaac aatcactgat ccttcataac cagcgttttc
    48421 agcgatttga cgaattggtt cttctaaagc acgaacaacg atcttgatac ctgttgccac
    48481 gtctccttct gcttctacag cagaaacttt gctgataacg ttcactagag ctgtaccacc
    48541 acctgagacc attccttctt ctactgcggc acgtgtagca ttcaatgcat cttcaatgcg
    48601 taattttaat tctttcaatt ctgtttcagt agctgcacca actttaacaa cagctacacc
    48661 acctgctaat ttagctaagc gttcttgtaa tttttcgcgg tcaaaatcag aagttgtttc
    48721 agcaatttgg tttttgatca attgcacacg agcttcgatt gcttcttttt ctccagaacc
    48781 ttctacgatc gttgtattgt ctttatctac aacaacttta gaagcatttc ctaagttttc
    48841 gattgttgca tctttcaatt ctaaacctag atcgtcagta attactgtac cacctgtcaa
    48901 aatagcgata tcttcaagca ttgctttacg acgatcgccg aaaccaggag cttttacagc
    48961 tactacgttg aatgttccac ggattttgtt caatacaaga gttggcaatg cttcaccatc
    49021 tacatcgtca gcaataatca acaatggacg tgattgttgc aagatttgtt ctaacaatgg
    49081 cagaatatct tggatgttag agattttttt gtctgtgatc aagatatatg ggttttctag
    49141 aacagcttcc attttgtcgt tatctgttac catatattgt gataagtaac cacgatcaaa
    49201 ctgcatacct tctacaacat ctagttctgt ttcgatccct tttgattctt cgattgtgat
    49261 gacaccgtcg tttccaactt tttccattgc atcagcaatc aaatgtccaa ctttatcaga
    49321 acccgaagaa acagctgcta cttgtgcaat ggcttctttt gaatcaacaa cagttgagat
    49381 attatgcaat tcttcgactg ctgcttttgt tgccaattcg atcccgcgac gaatgcctag
    49441 aggattagca cctgcagtta cgttttttaa accttcgcga acgattgctt gtgtcagaac
    49501 agttgctgtc gttgtaccgt ctcctgcaat atcatttgtt tttgatgcta cttctgatac
    49561 aagttttgca cccatgtttt caaaatgatc ttctaattcg atttcttttg cgatcgtcac
    49621 accatcattt gtgatcaatg gagagccgta agatttttct agtacgacat ttcggccttt
    49681 aggtcctaat gttactttca ctgtatctgc taatttatct accccgcgta acatagctgc
    49741 gcgtgcgtct tctgcaaatt tcaattcttt tgccatgatt gattcacctc attgattatt
    49801 tttgattatt tttttatctt ccaaatttac tcaacaatcg ccatgatatc ttttcctgcg
    49861 acgatcagat attcttttcc ttcgtatttt acttctgtac cggcgtattt ttcgaacata
    49921 acttggtcgc ctgcttttac agcggcagga actttttcac cgttttctaa caaacggcct
    49981 tcaccgactg caacaactgt cccagtttgg ggtttctctt tagcagcaga agctaaaacg
    50041 attcctccga cagttttttc ttcttctttt gcgacttcga taatgacgcg atcacctaat
    50101 ggttttaaca cgataaatcc ctccgataac tcttttttta atctgttagc actcttaacc
    50161 ttcgagtgct aacctacatt tttaataata ctcattctcc ctcttttttg caagctttta
    50221 aatcaaagaa aagaaatttt attctgacag ctgcttattg aaattatgat ataattgaaa
    50281 tcaatcgata taagcttgcc ttgcatgctt gttatccctt aaaaggagga attacatgtc
    50341 tttaaaaaaa tatagcttcc tcagcatttt ttgctatggt ctaattttta tctcaccctt
    50401 gatcctagct tcgataggac ttgtaaaatc tacgtctgaa ttgatcacag caactgcagt
    50461 catgtatata ctaggcgccg ttgtcctagc tattttttat tttaagcaac gtgaaccctt
    50521 agcgatcgaa tcagcggtaa aaaaagcttc tgctccaaag attgttattt atggattggt
    50581 tggaatcttt gtcgcattga ttctgcaaag catcgctgtc atgttagaat ctgtattgtt
    50641 tggcaaagca accccttccg agaatacaca aaatatcatc cagatgatca tggaagcacc
    50701 cgcttttatt ttggcgacaa ctgttgctgg acccatcatg gaagagttcg tattccgccg
    50761 cagtatactg gggatcatca gtcgatattc aaatttttgg gtcggagcag tgatcagctc
    50821 tttgctgttt gcgtttgctc ataatgatgg tcatctgcta atctactttt tcctaggttt
    50881 cttcttcagc ctagagtaca aagcaactgg tcggatctgg acatcgatga tcacacacgt
    50941 aggaatgaac acattagttg ttcttgttca attagctgtc caaaaagggc tagtataaac
    51001 atgactataa atactcctgt catcctagat tggtgatttt cccaatctaa agcgacagga
    51061 gtatttttgt tttttagtcg ttcatctttt ctgctgctcc atcatttttt gtctgtcttt
    51121 cctttttgca gtttgcagaa tctatttttt tcaaaaatgg gaaataaatg aaaaaactaa
    51181 ttgacaacac gataccttgt aaaatcgctg caggcactcc gcctactaag aaacccgaga
    51241 ggatcggcgg tgttgtccaa gggacttgga ctgctgtaaa taaaggaacg aatccaaaat
    51301 ataaagcgga ataggtaatc aatcctgaaa cgaccggtgc gaaaataaaa ggaacagcca
    51361 tcatcgggtt catcacgatc ggtgtggaaa aaataattga ttcattgatg ttgaaaaagg
    51421 ctggtaaaag agcaagattt cctaattctc tgtatttctt cgatttcgca aaaaccgtca
    51481 taaaaaatac agcccctaac gtcaatccag cacccgtaac cgtcatgaac tgatcgagaa
    51541 attgctgagt aacgatatgt ccgccatttg ccaccgtcaa ctcttgtcct gaggctaaga
    51601 tttctgcatt tgctaatgaa ttcgcttgca agacgggact aaccaaccca ttgaccatcg
    51661 cagatccatg aagaccaaat aaccataaaa acggaacaaa aaaacctaat aaaagcacac
    51721 caaacaacgt gtctatgaac gattgaatgg gaagctggac atatgtccat actccttgaa
    51781 caacagtcat tctgaacaaa tgttgaaaaa ccgctgaacc gataagcgta aaagtgacta
    51841 taacaatgat tggaatcaat gaagtaaaag actgccaaac attttcaggt atcttctctc
    51901 tttgcttcca ccctttcgca agtcttacta aacgtgcata gccccatccg atgatgagcc
    51961 cagttgtaac tgctcctagt atcccttcag cacctgtcca gacagtatct atctgagtat
    52021 ttagtggctg aataagcagt agataagcag acaaggctgt tatgccgact ggtaagcgat
    52081 cgatgtcttc ttcttttgca taagcgtatg caatggcaaa aacgatccaa aaggctatca
    52141 catcaaatgt agcctggtat acttgtgtaa aatacataga aaatcctgtt ttgtctatcc
    52201 actgagcgat gtaatccaca ggaaaatgag caaataagaa aaacagcgag ccaacaataa
    52261 ttattgggat cgtgtaagtc attccattac gcaaagcagt taagggtcta agattcacaa
    52321 cagatgttat cattggtaaa aaggcacgat ctaaccactc tttcatttct tatctccttc
    52381 aacttctatt tttatcaatc agttttctta atttaactat acccaatgat cacgaaccat
    52441 gaaaggaatg cgcactgagc ttttcaaaaa tcggattctt tgacgaatat tcagcaactg
    52501 cttacaatgg acaggaataa aaaaggaggt tttttatgaa aattgcactc attggagcta
    52561 ctggccatgc aggttcattt attttagacg aagcattgtc cagagattta gacgtgacgg
    52621 caatcgtgcg tcatccagaa aaattgccat cagatgttcc gtttatacaa aaggatttat
    52681 ttgagttgac aactactgat cttgcatctt ttgatgttgt cgttgatgct ttcaacgctc
    52741 ccaaaggaaa agaagaaatg catcaaactt ctttgtccca tttgattgag atgcttgaag
    52801 gaaccgatac acgcttaatt gtcgttgggg gtgcctcttc gttattctta gatacagaaa
    52861 agacgactcg tatgattgac caagtaccta aaaatgcacc cttctatcca acagctttca
    52921 atatgtacga aggattgtta aaattgaaac aatctaaaaa tctacattgg acttatatca
    52981 gtccagcttc tttatttgat ccacatggca aacgtacagg tacttatttg ttaagtgatg
    53041 attatttaca aagaaacgct gctagcgaca gtacgatcag catggcagat tacgcgattg
    53101 cattagtaga tgagatactg gatgagaaac atgaaaagca gcacatcagt gtagtcagtc
    53161 gataatacgg ttattttttt aatatgctaa aaaaataaaa atagaagata aaaccaaccc
    53221 tgattgattt tatcttctgc tttttatatt tttattgatt gaactgttta aatcgaaaat
    53281 agctcagttg cttcatctgg atctgtatca tagatttcaa aatcatgtcc aacgccaaaa
    53341 tctttttctt tcaaaacatt ttccattgtt aaatgagcaa gttctttcat attgttttcc
    53401 ttgctaagat ggccaagata gattcgtttg gtccgattac cgatcacatc agtcatgaca
    53461 agtgcaccgt cttcgttgga aagatgtcct ctatctccta aaatacgctg ttttaaattc
    53521 cacggataag gtcccatgcg taacatctca agatcatgat tactttccat caaataacca
    53581 tcggcatttt caatgacacc tcgcatataa tcactgcaat atccggtgtc cgtcaacatg
    53641 acgaatgacc gattttcttt atggaaacga taaaattgcg gagccgctgc gtcatgggag
    53701 acgccgaaac tctcaatatc gatatcgccg aatgttctga ctttccccat ctcaaataga
    53761 tgtttttgtt ccactggtac atttccaatc agtggttcca tcgcttccca agtctgctga
    53821 ttggcaaaaa catccaaatg atacttacga gccaagacac ccacgccatg tatatgatct
    53881 cgatgttcat gcgtcacaag tattccatct aattggtccg gtgtccgatc aatttgagcc
    53941 atcaaggaag tgattttttt accgctaagt cctgcatcga ccaaaagccg ttttttttca
    54001 ctttcgatat aaagtgaatt acccgaactt ccacttgcta ggatactgat tttaaaagct
    54061 ggtttttctt cagccatttg actcctactt cccttcttac tttgtaaact tttttcatta
    54121 tactcgcttt tttattgatc ttccactttt ggtacagtat tgtttgtcac gatcgtattg
    54181 ctgaaagcgt tgatgctctc aatttggacc actttatcgc ttgtttcgat cgctacatgc
    54241 caaacaggaa catacacatt tttttcccgg actttcagaa tacaggaata agctagctga
    54301 cgccataaaa tttttgatcc tcttggtatt ttgttattga tgtacaatgt attgattgct
    54361 tcctcttccg tatacagttc cgtcttttcg cgcagctgct cgatatcaga taaatgcgtc
    54421 tgcatgtatt tcgtgatgct ataatcatta tcgttttttt ctaaagaaag aacgattttt
    54481 gctgtatcat cattgaatgg gatgccttca tactcttggc ttgcttggat ttctagtggt
    54541 tcttcttcta aatgagaaaa gtttttcgtg tacacataat cattaccgaa taaaatattg
    54601 tctttgttag ataaaaaatc ttccaaggaa ctactaattt tatctggatc gatcacgttc
    54661 ttttcagcat ctgaactgga taaagatttc gtcaaaacat caccgtctaa aaacacacca
    54721 cttgccagca agccgctttt accagccttg cgataattcg ctaatgaagt gctaagattg
    54781 gtctgctccg cgctaagata atagccttgc ttgttctcag acgaaatctt ccccgtgtaa
    54841 gtgatatcgt cgctagccag acgttgttgg attgattcag tctgattaga gcgaatgaca
    54901 ttgttttctt ggtataccgc gtcatgataa ctgctgaata aaaacacatt caaccctaaa
    54961 aaagcgacga aaaatatcca ttcgattttt ttgaaatcca attaattcgc ctccaatcca
    55021 ggtaattttt ccagaaggtc gttagctgaa taccagttat ctttatattt cacataccat
    55081 tcaggtaaga gatcaacgac acgattcgtt tctttgacat tttgccatgt atagccaacg
    55141 atcactgaac caatcttgtt gctatctgct cccttagctt ttaactgctg gatgatttca
    55201 ttcgtacttg caagttctac ttcttcgtct gaaggtatcg gcacttgaat cgtattcaaa
    55261 ctggtttgga tattcacttg cacagaacca ttcgtttcac cgccaacttc cacaccaatc
    55321 tgtcctctgc tgtctgtccc aaaaaccggg aagccttcca cgaatatccg ataatcgatc
    55381 gtattgtcac tgcggtcaaa atatctgagg ttccctaaag aactgcctaa tttgctaata
    55441 taggggaaac tttcagaaaa tacactagca ttttcttttt tctctggcag ctctccacga
    55501 aaatcaacta cttgcgtttc cgaatggata gacattgttt cattcccacc gtaaaaaatc
    55561 aattcattgc tttcatcatt atttttcacg ctatctggtt tctggaaaaa agcatttcta
    55621 aaaagtgtat agggttgctg tgacaggata taactatact tcttcagttt gatcgaatgt
    55681 ttcgtagcat attgcgtgtc gatcgtcgat ggttctgttg tcatttctgt ccattttgtg
    55741 tcttttgctg ttaattcttt attgatatct gtccaattca acgaaatatt tgcttcatat
    55801 acgagatggt tcgaataatt caaaaatcgg atttttttat ttccttgatc gaattgtatt
    55861 ctagtaaaat acatattttc gctttctttt gccaagttcg acacatctaa atctaaatca
    55921 aatgcttcct tgtattctct tagcaaaaaa ggcgcattat aggccaactc aaaaccagag
    55981 ttcagatttt tcacctgttc aaactgtgat ttgtctcctg aaactacttc agaaagcttt
    56041 ccaaatcttg ctttatcgat gatcaattgc aatcggtaca cgagattttc actgttggtt
    56101 tcttggatca ttcccgaatg gtgccatgta acatgaagcg gaagaaagac ttcggaagtt
    56161 tttcgattgc tttgagactc gatcatttga gtgtttgtct catctacgtt gattgaactt
    56221 tttcctgctg ggctcagcca gattgcataa gagaagtata tgcttaacgc aaccatcaaa
    56281 atgagtccta tacgaacgac tttttcagag attctcattc ccaccattcc tcctcataag
    56341 gttcatatgg cagcgagata taaaaagtcg acccttttcc ttccttgctc tctgcccaaa
    56401 tagcgcctcg atgcgcctta atcacttctc tagaaatagc taatccaagt cctgtcccac
    56461 cttgtgctct tgcacgtgct ttatctactc gatagaaccg ctcgaacact cggttcagat
    56521 cttttttagg aatccccaat ccttgatcgg tgatacttaa aatcacgtta ttatgcgttt
    56581 ctaataagcg acaagtaatc gttcccccat ctggtgaata cttgatcgcg ttattcatga
    56641 tattatcgac tacttggatg attttatctg tatctacttc gacccaaaga tctctttgcg
    56701 taaattcacg acggatctta taattttttt cctgatttcc caccatcata tcaaaccggt
    56761 caagaacaaa attcactaac tcgttgaaat tgacatattc taattgaagc tgggtattac
    56821 ctgtatccat gcgagataga tttaagaggt cattgatcat tcgaatcatt cggtctgttt
    56881 catctagcgt taccttcaaa aaatttggtg ctacttcctc gtctttccac gcaccttcgc
    56941 tcaatgcttc gatataacta cgcatacttg tcaacggtgt acgaagttca tgggaaacat
    57001 tagagacgaa ttcacgacgt tcccgttctg ttttttcttg ttctgtcaca tcatgcaaga
    57061 cagctactaa tccgctgata aaacctgatt cgcgacggat catagaaaag tctactcgca
    57121 agatcatctg atcactttcc aatgtactgg ttgaacggtc aagtaccatt tcgtttggat
    57181 cttctaataa tttccgcaac gtatattctt cttcgatatc taaaagtgtc aaaatcgatt
    57241 gtccgattgc ttcctcgttt ttgatatcta gcaaggaaac agccatatca ttgatcgtaa
    57301 tgactttacc cctgcgatct gtcgcaatga cgccgtctgt catatgggat agcacgctat
    57361 ccaaacgatt gcgttcagct tccattgtat cttgagcttc ttcaatacgt tcagctaact
    57421 gattgaaggt atcagccagc tgtccaagtt catcttgtcc atgcactttg actttccgac
    57481 tgtagtctcc tctagcgatt cgaatggctt gttctctcat ttcaccgatc ggctgtgtaa
    57541 tcgaacgagc aactaaaaca gatacgatca ttgaaataaa tgccgcaatt agtgaggcag
    57601 taaagaaaat gactgctgtg ttattgattt ccgaatattt ttgttcgata ttacttttga
    57661 cgtaaagagc cccgataacg gcatcgccgg tcggggacag gatcggttga acattgatat
    57721 tcacccgttt atcgtcgtta tctaaagcaa cataacgttt agtcgtgaaa tcattgatat
    57781 caacataatc atttttcttt cccacagcgc tttgttcatt gacatcggtt gttcctcgga
    57841 tgatcccttt ttcgtcaacg acccgtattt cgatgatatc gctagtcgca ctgttttcaa
    57901 taaatctttt gatattcgca tttacctctt catcatctgc attatctttt cgattcaatt
    57961 ccgggctgat tgttgttgct aatgattcca ccgtttgatt catatcttta ataaatgtat
    58021 tgatcgtgga acgttctaaa ccacgtataa agtaggcacc aataatttcg atcgagatca
    58081 ataaaatcag aataaaggta atagcgatct taaaattgac tgatcgaaaa aagcggactt
    58141 ttcttttcat ccaattactc ctgttctggg ttacgcaaat agtaaccgac accacgacgt
    58201 gtcactaaat aatttggatg gcttggatta tcttcgatct tttcacgcaa acgacgtaca
    58261 gttacatcca ctgttcgtac atctccaaaa taatcataac cccaaaccgt ttgtaataaa
    58321 tgttctcttg tcatcacttg tccaaggtgc ttcgccaagt aaaagagtag ttcgaattca
    58381 cgatgtgtta actcgattgt ttctcctcgt ttcgtcacca tgtaagcttc tggatggatc
    58441 gtcaaatcac caatcgtcaa ttccgcaggt gctgcctctt ctggttcctt cgcagccgtg
    58501 gcaccgcgac gaagatttgc tttgacacgt gcaactaatt ctcggttgga aaatggcttg
    58561 gtcacgtaat catcggcgcc taactctaag cctaggactt tatcaatttc agaatctttg
    58621 gctgttacca taatgatcgg catatcataa gttttgcgta cctcacgagc aacttctaag
    58681 ccgtccattt taggtagcat caaatcaagt aaaatcaaat caggttctac ctcagcaact
    58741 ttttccaagg cttcttcccc atcatatgca gtataaacgt catagccttc tttggctaaa
    58801 ttgaatttga cgatatccga aatcggcttc tcatcatcga caactaaaac ttttttcata
    58861 aagagccacc tcttatatat tattgtcata ccttctcatt ataccgtatt tctgcttttg
    58921 tttcatcttt gcctataaaa caaaagagaa aattaaaatg aaagtgcaaa atactttatt
    58981 attttttgtt ctaacttcaa taaaaaagga aaacactgat tttcacctca gtgtttcctt
    59041 tttttgattt atccttctaa aacttgtaca ccgacaccca ataatcccgg accagtgtgg
    59101 actactaatg ccggagaaat ctggccaaag tagatttggt ttgcttgtgg gaactctctt
    59161 ttaagtctcg cttccatttc aattgcttct tctttcgcat ccccatgggc aacagctaag
    59221 ttgaagacct ttgcattacc gattttttct ttgacgtaag caaccgtttt atccaaactt
    59281 tttttacgtc ccctagcttt tcctactgtg taataaatcc cttcttcatt acatgagatg
    59341 attgggttaa gctttaaagc ggttcctaca atagaagcaa ctaaaccgat ccgtccacct
    59401 ttttgaagat attccaatgt cgcaacatta aagaaaactt ttgttctttc gactaactca
    59461 ttcagattac tgatcagttg atcccattct actccttctt ccagccattt tgctgcttgg
    59521 atcgcagaaa aacctgcacc aataccaata tttttcgtat ctagtacata tgcttccaat
    59581 tcttcttgct gttgtgcaat taaacgaacc acattatatg tcccgctcaa tccactggaa
    59641 attgttaccg caagtacctt ttcataaccg tcttttttga tttgctcaaa gatttctgtg
    59701 atcgactcac catccggcaa agatgtacca gggatttcct ctggcaaacg ttgatagatc
    59761 tcttcagggg taatgtcaat tttatctgta tatacctgat ctttataaat aattttcaat
    59821 gggatcatat acatctcgta tttttttatc aattcttctg gaacatccgt acctgaatct
    59881 actaaaattg cgattttatt gtttttcatt tgttttctcc tcttttaaat attcggtttc
    59941 tagaacaatc gttttttccg caagaagttt cattgcaaac gataaagttg ccgctcttac
    60001 agcaatatat tctacagggg ttgcctggtc gaatttttgc ggatgagatt taccttgtgc
    60061 taattggctg accatccaca cagctgcttc ttgttcatcg caaaatagat tataggcttc
    60121 acgaattcct actgtttttc cttggaacaa tatcccttct ttgatttcag gaatagtcaa
    60181 tacttgtttc aacgtagtga ttgcaagtaa aaatgctacg tgttctttgt tatatctttt
    60241 tttaactggt gctggtatca aacctagttt aacgtaattg tttaccatag aagaggtaag
    60301 caatggatgc ttttctcctt gtatcacagg tgaaagataa cgatcaacta acgttctgac
    60361 ttggtccata taaagttcta agtcagggag ttcttcccag tgaggcagac ggaatgtttc
    60421 tagttccttc ccccactcca ttaattgctg acgcattttt tccatcataa ccgctccttt
    60481 tttcatttca taaacatgat atcagactta tgataaacat tcaatctagt tgctttaact
    60541 aggtgatttg attagcggga ttccatgaaa acaaaaaaca agaaggctaa gccttcttgt
    60601 cttttatcgt taagaacagg agacccgccg gggagacggc gggaaaggag taaaaaatga
    60661 aaaaagtgtt agggtttgtt ctcttgcaag aactttgttt gttggtatgc ttatataata
    60721 gcgagcaaat gtgaataatc tatgaatgat ttcctataag gaagtaaaga tttttctgat
    60781 tttctttaaa tgtagtcttg cattttaaaa ttcttagtgc tactattatt aagtactttt
    60841 tgtacgggcc gttagctcag ttggtagagc agctgactct taatcagcgg gtcgcgggtt
    60901 cgagcccctc acggcccatt gggtgccaaa cccacggaac aggtaaatgt tggtgcgaaa
    60961 gcatttcgaa aacatttacg ctgttctttt tttatttttc aaaataacta gggcttttta
    61021 tttttaactc aaagtccact gtagcccttt ggactttgag ttaaaaaaga agcataatta
    61081 tgaacagtaa agtttctaat ataacaataa ttcgagaagc tttgcaaaat catgctgcca
    61141 atgaactctc taaacatata ggattaaatc taagtactat aaaaggttaa aaagtgaaga
    61201 atatttaata gaaaaactaa atttacttgc tgctttcccc ttttccatta atacaacaat
    61261 cagagagtgt ctttttttcg aaaaaattag aataaataaa agacacgtag tttttttatc
    61321 tacatgtctt ccatcgacta caagttatca tttctcaaaa cgtatttagc ttttaattga
    61381 ttaatcgttt cacgttgtct acacattgcc taattaagat tttcttatta gaagcatcaa
    61441 tcccgcaata atacctaaaa ctccaggaat tacccacaat aatgttaaaa gaaaaaatgg
    61501 aataatagat aaaatgagta aactaatacc atagaccact gttttttgat tttttcttac
    61561 taaaaaacct attatgaaaa tcattatcga aaaaactagg gtaattcctc caaataaaac
    61621 aacattcccg cttagattga ctaacatatc tatatcctta ctggttagtt ctgtagtatt
    61681 taaagtagtt gaatcaatag aagatagaaa cataccaccc atcaatgcta aaagactaca
    61741 taaaatccct gttattccac taattaaaat caaaataaac tctgtttttc tcttattcat
    61801 ttaattctct ccagattaag tgttttagaa ttagaaattt tttgaccata gttaatagct
    61861 tctaatgaac gaataacaac cactgacgtg ttagaaaaat aatccccaat atgttgtttc
    61921 ttgggcgtga aagcagccac caaataccca atttgaaata caaaattttt taatataaac
    61981 ctacatatac actcacgaat aaagatagtt tgccaactta gctctttttc ctcaaagctc
    62041 actactctta tcccaaaaat cattttccca attgtttgtc ctgaattcag cttagtcaaa
    62101 agtacaaaat aggttaaata aatagctaat gacgctatac cataaaaagt aataatggat
    62161 ttgtcagatt ccagtcccat gattttgtaa aagagaccta tagtagcatg ggtgatgatg
    62221 ctaatacaaa ttaaatcaat aagaaaagca aaaaatcgta accaaaatcc cgcaaataca
    62281 taattaggca aagtttcaat gccactcttc ttattcattt tatagccctc catagtaata
    62341 cattggtttt ggagtatcag aaaatccaag tttttcaaaa actgaaatta cacgatctga
    62401 tgtagttgcc tttagccctt gccattcagc aatcttatta cctaaccaag aagaagcaaa
    62461 gccagttgtg tcatttttat attctataac cgtcgcatca gttaatttct tctctttttt
    62521 tagggaatct aatgcatctt cagagtaacc aatttgatcc accaacccat tttctactgc
    62581 ttggttacca tcataaattc ttccatccgc aagttctttc actgcattag tatccatatt
    62641 tcttcctttt gctacaacat tgacaaaacg atcataagca ctcatcacaa attcctgtat
    62701 aactttattc tcttcctctg aaggtttgtg ttgaggtgac atcatatctt ttaatgcacc
    62761 acttttatac gtcgcatcag ttacgccaag tttttctaat aagccagaat aatttaaacc
    62821 agaaataatg acaccaattg atccagttgt tgtttcttcc gtagcaaata ttttatctga
    62881 accagcagaa atgtagtacc ctccacttgc cgctgtattt ttaaatgcag tatagatagg
    62941 aatatctagc tttttaattt ttgccatttc tttagcaatc tcagcacttt catatattcc
    63001 tcccccaggt gaatttactt ctaataacac tcccttaacc gctttgtctt cttgaatttt
    63061 ctttagctgt gttaaaaaat tctgatggtt atattgttcc ctactaaaaa gattgctttc
    63121 acctgtatct gcaataactc cattgacgga tagtttgaca atctttttat ttgatgcgcc
    63181 ttcttctaac gtttcttctg tgatttcgtt agatccgtaa aaaatcttat ttattcctgt
    63241 tagggttgta tttgatgctt cctctttatc ttttcctgga attagcgcga cgataatgga
    63301 tagtaacaat aatccgcacg ctactcccac agcaatccat cttcttttat tcatataaac
    63361 tcctcctata ttcttagtaa aaataattat tttttattta gaaacacatc gacaatttgt
    63421 tgccaatatg ttttttctcc tacaattaaa gacaactctc cagttaatcc ataacgaaac
    63481 ggaaacgatt gagttgtttt taacgttcct ttcactgcat aaaaaacacc tttctcagtg
    63541 gcagtagaat tttctgaaat ttcatctaaa gttccgtcga tcgtaatcgg agccccacct
    63601 ttttgatcca atttaaaatg aaccttcatc ccttctttaa tataggtact ttcattcgca
    63661 ggtaaaagtg ctgtaaattt catttttgat ttttcgtgga tcggataaat ttctgcaagc
    63721 actgttcctt tggctagttc tgcttgtccc tttgtttctt catttaaatg aaccgttcca
    63781 gatacaggag cttttagtat tccttttgaa agttgatcgt ccaaactttt gataactgac
    63841 tggttcttct cttgtttttc atttagctct gtaactgttt gttttacagt tgttattgct
    63901 tgttctagct cttgctttcc tttttccgtt tgactaattc tgtcattctc agaagtagtt
    63961 ggtttaacta atttagcttt ttctgactga tactgttcta cttctttttt tagttgctca
    64021 atttgttcat ttatgcttgc tgaaattgtc agtttcactt gatttttttg atcatcagaa
    64081 acattattta gttgttcttg ccaattctca tagtttgcca tcatttcttt tgaaaaatct
    64141 tttaatgatt gattattact ccaagctacc tgtacttgtt gccattcttg taatttacta
    64201 tctgaactct caatttgttt ttctagttgt ctttttgtct tttcgtatac ttctaattgc
    64261 ttttgatcat ttaattcact ttgcttcaaa gcgtagcgga gtgattcttt ttctgacaac
    64321 atgctcttta attgattact gtagccaaaa ctgtcatccg ttgaaaacaa attttttcct
    64381 ttacttatac tttcaataaa tagctgagcc atctttattt gttgagtaat acgttggttt
    64441 tcttgttcta tctgagcctt ttcattctct attaaagaac aatccaatct aaccaacaca
    64501 tcgtccttat gaacaaattg attttctttt aggaagtttt ccattatttt agcttctaat
    64561 ggcacttgta acttctctat tttttcgggg ttcaattgag ccgttgtccg aataacaacc
    64621 tcttttttag caaaggttaa aaacaatcca ataatacata aaaaaagaat tacagggtat
    64681 aacacccaac ggtagaagct atgatgttgt ttagtataga tactagaatg ttcgaaccaa
    64741 ttattattct gcataaattg tttccctctc ctagatttcc cataattttt gatagatacc
    64801 atcggcaaaa cgaagttcat cgtgcgttcc tttttctacc aaatgccctt catggagaac
    64861 taaaatttga tcacatgctt ttgctattgg taaatgatgc gcaataaaaa taatcgtttt
    64921 ttcttttaaa ttgaggagat aattcataat tgcatgttct aacaaggtat ctaatccact
    64981 agttgcttca tctaaaatta atatgtccgg attttttaat aaagcccgtg ctaaggctaa
    65041 tcgttgtcgt tgccctcctg aaagattatt tccaccttct tccaacaatg tctcgtaacg
    65101 taatggctgt tgattaataa attcatttag ttggactatc tcacaaactt ctattatttg
    65161 ttcgaaactt ggtgggctat ctaaaccgaa tataagattc tcaatgattg ttccactaaa
    65221 gaaaaatgac tcttgaggca aatatgtcac tcgatcgcgt aatatctgaa aaggaatgtc
    65281 taagtgatta atgtttccat aatggatcga tccttctgat gcttcataaa aacgaacaaa
    65341 taactttgca agagttgatt ttccagaacc actaactcca actatagccg ttttactgtg
    65401 cgaaggaatt gtacatgaaa tttcgttcaa cgttggggcc ttcatgctat acgagaatga
    65461 aacgtttttt acagtgatat cttgttgaaa taattgttca gaaagcatat attcaaaaga
    65521 ctggttcgat ttttctgaat caatcgcgaa aatttcattt aaacgattat ttgcaacttg
    65581 agccgtttgc attttcactt gtaaattaat aatgttttgt aaaggatctg tcaaaaatac
    65641 tagtaaagca ttgtatgtga taagttgacc tagactaatt ctattatcca taacaaaata
    65701 agaacctagc cataaaatta atgcactgct aattaattga atcaaatgtt taattccttg
    65761 ttgaatatta tcaagcgtaa ccgtacgaaa tgattttttc attaatttaa caaattcatg
    65821 gtctatacgg ttatatactt tctcttcacc gctatatgct ttaattgttt ctattccttt
    65881 aagactttct atgatactag aatttacagc tgcaccggct gccatttctt ccctgttcga
    65941 tttttcatag cttttcacga aagcgaatat tgcaactaca taaaaaggca aagagcatag
    66001 agtgattaaa aacaacgtac tattctgaat agctaacgta accccaacaa gcaacaccat
    66061 accaatatct aaaaatattg ataacgtagc acttgctaaa gcatcaataa tcttactggc
    66121 atcaagaaaa cgagaaataa tttcccctga ttttctcgta gcaaaaaaat tcattggtaa
    66181 agttaacaca tgtttgaaat actgaagcat aattgccata ctcattcgct gtcctaaaat
    66241 aataagaaga taactacgac tatattcaaa caatacccga aataaataga cagcgatcaa
    66301 gccaatggaa attatattta aagttgaacg tgcttgatta gggatgaaaa aatcaagaat
    66361 accttgaaaa taatatgagc ttcctatgcc aaaaaacgta atgaaaatcg atgctaaaac
    66421 gatgtggaat actaatcctt tttgtttcca aattataggc aaaaaagaac ttaatccagc
    66481 tactttttct ttaataggaa tataagtttc tctaggcata ggtaataaaa gaatacctgt
    66541 ccattctttt tgaaacgctt caatcgtttt tctatttttt ccattagcgg gatctgcaat
    66601 ccatagtacg tttcccttcg ttttataaat tacaacgtag tgcatatagc tgttatcaac
    66661 caatatatgt gcaattaatg gtaagataag atctctttct ttccagatct cactatctgc
    66721 ttgaatagcc atacaatcaa aagacaattt ttcaaatgtc tttttcaatc caaatgctga
    66781 agtaccctct aaatcagtcc cagataggtc ccgtaattta tgaatcggga tatcagtttt
    66841 aaaatatttt aaaatcatcg aaaggcaagc aacaccacaa tctttttcat cttgttgctg
    66901 cacaaaagga aatgatttta tcacttatct aacacaactc cttctaaaaa atttttatag
    66961 aaaaattctc aatatagctt taatacagtg aaaatattgt ctcataattc cacaaaaact
    67021 ttgaaaacag ttcttgaaca ctaacttttt cattcctgaa tcaaatttat tttggaaatt
    67081 ttactattca agaataaact tgataagttt caggaagatt tttactgtta atcaaatacg
    67141 tatgttacag tagcaccgta aaaaatatta tttaaagaag cgtgtttaaa catgcaaaat
    67201 gtaaaggaac taagtattgt ggaaatgcac aaactgttgg tggaggcaat aatgcttggg
    67261 gaaaattggg gcaagttgta ggtggactta ccacaggggc agtaggtggt gcaggtttag
    67321 gaacagcaat ttgcggacca gcttgcggtg ttgttggagg gttatatgga gctgtcgctg
    67381 gaggtgctgc agctggctgg gatgctcgaa aaaaataaaa aaggagaata agtagtatgc
    67441 atattaaaaa cacaaaaaca acatttatat tatcttcaga agagttgaaa aatattcaag
    67501 gtgggagtgc cgtgggagtt ttaggcacta ctttttctgg ggcaacagca ggagtaaaac
    67561 tttgttcagc cggaggacct tatgcaatag ctgcttgtgg tgtaggtgga gctttattag
    67621 gtgctggatt cagcatgtgg accggtgcgt aaacaattaa aaaatatcaa acctcaagat
    67681 tggtttgtgt cgattacgaa cataggtatg ttctttatct tttcaaaagt ttggaatgga
    67741 cagaactttt tactaaagct tcttgtgcta gtttttataa ttatattaaa tttttatctt
    67801 ttattaaagt cagataaaaa ttctttggaa atgtaatggt agagatattt ttacaaaagg
    67861 acttgcagcg ctgtagttgg tgctgcagga ggacctattt cggccgttac aggaggtatt
    67921 ggtaatgtac ttgtcggatg tgcattaaag cctgttcctg caagttaaat ttataaaaac
    67981 aggaaagtaa ttatctcaaa tcctatcaag tacaatcata ggattgcact tgacaggaca
    68041 aattttttaa ctaaaaaatg aaaaacactt tctaaaaatt agtagaattt gccaaatctt
    68101 tcgcatcttc caccatacat ttaattttaa ttccccttta tggtacttaa taaaaacaag
    68161 aaaagaacta ttcatttggc ccttttttgg ttccagacca tagaaatatt cctccagtaa
    68221 ctaatattgc tgttatgcac gtaactaata acagctataa aaattggtac aactttaata
    68281 ggattcagaa aaatttgcac tagtgaagga atctatttta ttagaaaaat gcaatctccc
    68341 taaagaaaaa accactaaac agtgctttct ttcaacttct ccttaatttt ttgtgctttc
    68401 cctaaagtaa agtttctggc caactctgaa tcaaaatata gtgaacgttt tttaaaatct
    68461 atttctttaa cgttaaagat atttactaaa cacgctcgac taattcttgt taacatagga
    68521 tatttctttt caagctcact aattcttcca taaaattcgt actgcccatc tttagtacac
    68581 aatgataatc tatgtggtaa ttttgaagct tctacaaaat aaatgtcatt tatatcaaaa
    68641 gtaaatgttt gagaacctat ggaaaaaaca aatgcttgat tcgtaacctg ttttgttgca
    68701 tctatccttt cttgcgctaa taacaataac tcaacaatct cgtttcgata ctcatctagt
    68761 gtttgatctt ttgttacaaa tcctaacgtt tcaactcttc tttttattgt tacgggtaac
    68821 atctcatcat gagtagttgt aaaaatgatt tttgcttgaa catcatattt tctgattact
    68881 tctgctaatt ctataccgtt aacttcatga tttaaatcaa tatctaagaa atatattcct
    68941 tgctttggtc ggaattgttt gaggtatttt ttaacttcta acggactttg tgttttcaat
    69001 actatctt
//
LOCUS       DAFBQH010000006        68909 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.006, whole genome shotgun sequence.
ACCESSION   DAFBQH010000006 DAFBQH010000000
VERSION     DAFBQH010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.006"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(108..632)
                     /locus_tag="LK694_000437"
     CDS             complement(108..632)
                     /locus_tag="LK694_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5051335.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(625..1098)
                     /gene="tsaE"
                     /locus_tag="LK694_000438"
     CDS             complement(625..1098)
                     /gene="tsaE"
                     /locus_tag="LK694_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBK5051336.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1221..2201)
                     /gene="pta"
                     /locus_tag="LK694_000439"
     CDS             complement(1221..2201)
                     /gene="pta"
                     /locus_tag="LK694_000439"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBK5051337.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2354..3034)
                     /locus_tag="LK694_000440"
     CDS             complement(2354..3034)
                     /locus_tag="LK694_000440"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBK5051338.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3160..4029
                     /locus_tag="LK694_000441"
     CDS             3160..4029
                     /locus_tag="LK694_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK5051339.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4055..4570
                     /locus_tag="LK694_000442"
     CDS             4055..4570
                     /locus_tag="LK694_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051340.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4617..5210)
                     /locus_tag="LK694_000443"
     CDS             complement(4617..5210)
                     /locus_tag="LK694_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5051341.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5235..5414)
                     /locus_tag="LK694_000444"
     CDS             complement(5235..5414)
                     /locus_tag="LK694_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051342.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5491..6000)
                     /locus_tag="LK694_000445"
     CDS             complement(5491..6000)
                     /locus_tag="LK694_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051343.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6096..6476)
                     /locus_tag="LK694_000446"
     CDS             complement(6096..6476)
                     /locus_tag="LK694_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK5051344.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6806..7555
                     /locus_tag="LK694_000447"
     CDS             6806..7555
                     /locus_tag="LK694_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBK5051345.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7739..9016)
                     /locus_tag="LK694_000448"
     CDS             complement(7739..9016)
                     /locus_tag="LK694_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051346.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9095..9739)
                     /gene="pcp"
                     /locus_tag="LK694_000449"
     CDS             complement(9095..9739)
                     /gene="pcp"
                     /locus_tag="LK694_000449"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBK5051347.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVSFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9938..10882)
                     /locus_tag="LK694_000450"
     CDS             complement(9938..10882)
                     /locus_tag="LK694_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBK5051348.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10884..11570)
                     /locus_tag="LK694_000451"
     CDS             complement(10884..11570)
                     /locus_tag="LK694_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBK5051349.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12044..12364
                     /locus_tag="LK694_000452"
     CDS             12044..12364
                     /locus_tag="LK694_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBK5051350.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12518..13423
                     /locus_tag="LK694_000453"
     CDS             12518..13423
                     /locus_tag="LK694_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5051351.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13420..14193
                     /locus_tag="LK694_000454"
     CDS             13420..14193
                     /locus_tag="LK694_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBK5051352.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14374..15798)
                     /locus_tag="LK694_000455"
     CDS             complement(14374..15798)
                     /locus_tag="LK694_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5051353.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16009..16455
                     /gene="nrdI"
                     /locus_tag="LK694_000456"
     CDS             16009..16455
                     /gene="nrdI"
                     /locus_tag="LK694_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBK5051354.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16520..18502)
                     /locus_tag="LK694_000457"
     CDS             complement(16520..18502)
                     /locus_tag="LK694_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK5051355.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18516..19412)
                     /gene="murQ"
                     /locus_tag="LK694_000458"
     CDS             complement(18516..19412)
                     /gene="murQ"
                     /locus_tag="LK694_000458"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBK5051356.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19415..20476)
                     /locus_tag="LK694_000459"
     CDS             complement(19415..20476)
                     /locus_tag="LK694_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBK5051357.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20687..21253)
                     /locus_tag="LK694_000460"
     CDS             complement(20687..21253)
                     /locus_tag="LK694_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK5051358.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(21281..22783)
                     /gene="eat(A)"
                     /locus_tag="LK694_000461"
     CDS             complement(21281..22783)
                     /gene="eat(A)"
                     /locus_tag="LK694_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBK5051359.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23041..24162)
                     /locus_tag="LK694_000462"
     CDS             complement(23041..24162)
                     /locus_tag="LK694_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBK5051360.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24288..25229
                     /locus_tag="LK694_000463"
     CDS             24288..25229
                     /locus_tag="LK694_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBK5051361.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25379..26275)
                     /locus_tag="LK694_000464"
     CDS             complement(25379..26275)
                     /locus_tag="LK694_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBK5051362.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26544..27884
                     /locus_tag="LK694_000465"
     CDS             26544..27884
                     /locus_tag="LK694_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5051363.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28102..28545
                     /locus_tag="LK694_000466"
     CDS             28102..28545
                     /locus_tag="LK694_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBK5051364.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(29049..30467)
                     /gene="nhaC"
                     /locus_tag="LK694_000467"
     CDS             complement(29049..30467)
                     /gene="nhaC"
                     /locus_tag="LK694_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBK5051365.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30870..32093)
                     /locus_tag="LK694_000468"
     CDS             complement(30870..32093)
                     /locus_tag="LK694_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBK5051366.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(32124..32963)
                     /locus_tag="LK694_000469"
     CDS             complement(32124..32963)
                     /locus_tag="LK694_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBK5051367.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(32980..33660)
                     /locus_tag="LK694_000470"
     CDS             complement(32980..33660)
                     /locus_tag="LK694_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5051368.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33662..34735)
                     /locus_tag="LK694_000471"
     CDS             complement(33662..34735)
                     /locus_tag="LK694_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5051369.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(34791..35029)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(35072..35305)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(35382..36053)
                     /locus_tag="LK694_000472"
     CDS             complement(35382..36053)
                     /locus_tag="LK694_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK5051370.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(36140..38818)
                     /gene="mgtA"
                     /locus_tag="LK694_000473"
     CDS             complement(36140..38818)
                     /gene="mgtA"
                     /locus_tag="LK694_000473"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBK5051371.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            39477..39914
                     /gene="ndk"
                     /locus_tag="LK694_000474"
     CDS             39477..39914
                     /gene="ndk"
                     /locus_tag="LK694_000474"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBK5051372.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(39936..40802)
                     /locus_tag="LK694_000475"
     CDS             complement(39936..40802)
                     /locus_tag="LK694_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK5051373.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(40962..43055)
                     /locus_tag="LK694_000476"
     CDS             complement(40962..43055)
                     /locus_tag="LK694_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBK5051374.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(43804..44412)
                     /locus_tag="LK694_000477"
     CDS             complement(43804..44412)
                     /locus_tag="LK694_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051375.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLITAYSEKVDKKVVADRLAEWKKTNEQVKKTTVSSQK
                     NESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(44571..45968)
                     /locus_tag="LK694_000478"
     CDS             complement(44571..45968)
                     /locus_tag="LK694_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK5051376.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(46000..46971)
                     /locus_tag="LK694_000479"
     CDS             complement(46000..46971)
                     /locus_tag="LK694_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5051377.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            47345..47770
                     /locus_tag="LK694_000480"
     CDS             47345..47770
                     /locus_tag="LK694_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051378.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(48258..48959)
                     /locus_tag="LK694_000481"
     CDS             complement(48258..48959)
                     /locus_tag="LK694_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBK5051379.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(48975..49415)
                     /locus_tag="LK694_000482"
     CDS             complement(48975..49415)
                     /locus_tag="LK694_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBK5051380.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(49415..49990)
                     /locus_tag="LK694_000483"
     CDS             complement(49415..49990)
                     /locus_tag="LK694_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HBK5051381.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(50838..51317)
                     /locus_tag="LK694_000484"
                     /pseudo
     CDS             complement(50838..51317)
                     /locus_tag="LK694_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(51476..52177)
                     /locus_tag="LK694_000485"
     CDS             complement(51476..52177)
                     /locus_tag="LK694_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5051382.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(52285..53694)
                     /locus_tag="LK694_000486"
     CDS             complement(52285..53694)
                     /locus_tag="LK694_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBK5051383.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(53742..55607)
                     /locus_tag="LK694_000487"
     CDS             complement(53742..55607)
                     /locus_tag="LK694_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK5051384.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAEADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHRGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KEGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(55728..56579)
                     /locus_tag="LK694_000488"
     CDS             complement(55728..56579)
                     /locus_tag="LK694_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK5051385.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(56889..57602)
                     /locus_tag="LK694_000489"
     CDS             complement(56889..57602)
                     /locus_tag="LK694_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBK5051386.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(57670..57996)
                     /locus_tag="LK694_000490"
     CDS             complement(57670..57996)
                     /locus_tag="LK694_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBK5051387.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(58119..58382)
                     /locus_tag="LK694_000491"
     CDS             complement(58119..58382)
                     /locus_tag="LK694_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBK5051388.1"
                     /translation="MEKQANPYDHKKMLTQQLRELEQDESIHRKAYNQVPPKVEYSLT
                     EEGKSLREILVALSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(58667..59920)
                     /locus_tag="LK694_000492"
     CDS             complement(58667..59920)
                     /locus_tag="LK694_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBK5051389.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            59981..60721
                     /locus_tag="LK694_000493"
     CDS             59981..60721
                     /locus_tag="LK694_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5051390.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(60807..61565)
                     /locus_tag="LK694_000494"
     CDS             complement(60807..61565)
                     /locus_tag="LK694_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBK5051391.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(61562..62590)
                     /locus_tag="LK694_000495"
                     /pseudo
     CDS             complement(61562..62590)
                     /locus_tag="LK694_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(62610..65777)
                     /locus_tag="LK694_000496"
     CDS             complement(62610..65777)
                     /locus_tag="LK694_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051392.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(66001..66885)
                     /locus_tag="LK694_000497"
     CDS             complement(66001..66885)
                     /locus_tag="LK694_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBK5051393.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(66980..67417)
                     /locus_tag="LK694_000498"
     CDS             complement(66980..67417)
                     /locus_tag="LK694_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051394.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(67634..68545)
                     /locus_tag="LK694_000499"
     CDS             complement(67634..68545)
                     /locus_tag="LK694_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBK5051395.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT        19759 a        14472 c        11868 g        22810 t
ORIGIN      
        1 gtttattcaa aaaaaatgga ctgcgacacg acttttgtca cagtcccttc gtgtgttttt
       61 ctgccttctc aaaaggatga cagtttctca ctcttttcct atttcttcta ttcaatcaaa
      121 taactcattt tatatacatc taaaaattca cctagatctg ttcttgcccc tctaggcatg
      181 accgcttcga tttggaagcc catttttcgg taaaggtgta cggctctttc attacgaact
      241 tgaacgtcaa gctccaatcg aaaaagtaca cccatttcct tcgcccaact gatgatttct
      301 tctagcataa gagtgcctag tcccattccc caatattcct gcagaatact gataccgact
      361 tctcccacgt gagacaaacg aaattgactg tctgccttga cagatgctgt tccaataatc
      421 gtcccttcat agattgcaag cagcaataaa ttattattgc tttcgtatat ataatccaac
      481 tcttcttcta aggtttctgg tggtaacagg agttctgctt catccaaaac taaaaattct
      541 gtctccttat taacttgtcc catcatcaaa agaatgtctt ttgcatctcc tggcactgct
      601 tcccgcaaga tgatcccaac ttcttcactc atgattttca gcccattttt ctagaattcg
      661 ctgtttgaag gtttcacttt gctccccgta tgagattagt ttcacatacc gcttttctaa
      721 atctgtatca cttttttcta aaatcagttc caaataatcc tccggcagtg cttcttctaa
      781 tagatttccc cattcaacaa cggaaagccc atcaccttca aaatattcat ctaaccctaa
      841 atctgctccg ctagctgcta cccgataaat atccatatga tacaacggaa tccgaccttg
      901 actatattct ctaataatcg tgtatgtcgg acttttgatc atttgcgtta tccctaatcc
      961 ttgggcaatt cctttcgtca acgttgtttt acccgcacct agatcaccag tcaatatcag
     1021 attgtcacct ggcattgcgg aatgaccaat tgcctctgcg aattgttctg tcattgttaa
     1081 tcctgatagt tcgatcattt ttcatcttct cccgctcttc tttctttttg ccagtatatc
     1141 gcgtaatgaa gtttttttaa agaaaaaatg aactagtcaa gaacagcagc tgctgttttt
     1201 ggctagttcg cgtgtatttg ttaatcaatc aatgtttgtg ctgctgtgat aatcgataat
     1261 ttatatacat cttcttcatt tgctccacga gacaaatcag aaaccggttt gttcaagcct
     1321 tgtagaattg gtccgattgc ttcaaagtta ccgaaacgct gagcaatttt atagccgata
     1381 tttcctgact gcaattctgg aaatacaaag actgttgctt gaccagctac ttctgaatct
     1441 ggcgctttca gttgtgcaac tgaaggaaca taagaagcat caaattgcaa ttcgccgtcg
     1501 atcatgtatt gtggcgctag ttttttagcg attttagttg cttcaactac tttgtctact
     1561 tctggcgatt tcgctgagcc ttttgtcgag aagctcatca ttgctacttt tggttcgata
     1621 tcgaacaatt ctgctgtttt tgcagaatct acggcgattt cagccaattc ttgcgcagtt
     1681 ggattcacgt tgatcgcaca atccgaaaag acatattttt cttgatctct tccacgtacc
     1741 atgatcatcg ctccgcttgt ccgactgatg ccaggctttg ttttgatgat ctgaagtgct
     1801 gggcgaacag tgtcgccagt cgaatggatc gctccagaga ccattccgtc tgcaatcccc
     1861 atatacgtaa gcatcgtacc aaagtaattc acatctttta aaatcttttc tgcttgttct
     1921 ttggatgcct ttccatttct acgttcaaca aaagtatcca ccatttcatc ccacttatcg
     1981 taattatcag ggctgatgat cgtaaagcta gatgttttga ttcctctggc atgcgctgca
     2041 tttcttattt cttcttcgtt ccctactaaa atgggttcca caagttcttc tgctttcaac
     2101 cgggcagctg cgcctaaaat acgagcatca gtagcttccg ggaaaacgat tttgatccca
     2161 cggcgaacaa ttttaaatct taaactatca aacaattcca caagatgacc tccagacgtt
     2221 tttatatgta ttatcataca ctattccaga aaaaaagaac agtttatttt atctgtatca
     2281 gtcaaaaatg ctggaaaaat taagtttttc acatacaaag cgaactgcac tataacagga
     2341 aagcgctgtt ttattacact gtttcaggca gttgccaatc aatcggttct tctcctaacg
     2401 caaggagcgc atcattcgtt ttcgagaagg gtttggaacc aaaaaagcca cgacttgcag
     2461 ataacggact gggatgtgga gatgttatga tgatatggcg actcttatcg atcattttga
     2521 ttttttcttg tgccggtttt ccccacaaga taaacacgat tggtttttcc cgttcattta
     2581 atttttcaat gatcgtgtct gttaatcttt cccaaccttt cccgcgatga gaatacgctt
     2641 gcccttcccg aacggtaaga acggtgttga gtaaaaggac tccctgttta gcccatgaca
     2701 ctagatttcc atgttgaaca ggtgcgattc caagatcatc ataaagctct ttatagatat
     2761 ttcttaacga tggcgggatc tttacgccag gttgcacaga aaaagataag ccatgagctt
     2821 gattttctcc atgataagga tcttgtccta aaatcaccac tttaacttct tcataaggag
     2881 tcaattccaa tgcttcatag atatgataca tatctggatg gatcttttgg gacgcatatt
     2941 ctttttttaa aaattctctt agatgcagat aataatcttt tgaaaattct gaggacaaaa
     3001 cttcttgcca actgttatga atgatttctt tcaaaaactc cacctcctaa taaaaagaat
     3061 aactcacatt ttcttcaatc acaagggaca atcttcaccc tcagcacttg attaatatgg
     3121 taaactagga acaagaagca gaaaggaacg ataatcatta tgatcaaatt aattgcatca
     3181 gatatggacg gcacattatt agatgcccat atgagtgttt ctcctgaaaa tgccgaggcg
     3241 atacgttttg caaatgatgc cggtgttgag ttcatggtcg ctactggtcg aaactatcaa
     3301 gaagctcgcg cagctttaga tgaggtagga atcgattgtg cgatgatcac tttgaacggg
     3361 gcacaggtat ttgataaaga aggaaattca ttgtttaccg tgtcgattcc aaataatcaa
     3421 gcaatctctg ttttagatat tctagatgct aacggtattt actatgaagt agcaaccaac
     3481 gatggtcttt attctgaaaa tcaagcgaaa cgaattgaaa gttttgcttc catggtggct
     3541 acccatttac ctcaccttac gtataaaatg gcgattgcca tggcctcagc taagctggag
     3601 ttattgcaca tcacatacgt ggacagtatt cgtgctattt tggaagaaaa gaatctggaa
     3661 gttttaaaaa ttatttgttt tcatacagaa gggccagctg tattaggtcc agttggaaag
     3721 cagatttctc ttttaggcga tctagccgtt acttcctctg gtcaaaacaa tatcgaagtg
     3781 aaccataaaa acgctcaaaa aggaattgct gttgcccatg tcgctcacga gcgtgggatt
     3841 tctttagaag aagtcatgac gattggcgat aacttcaatg atgtcagcat gctgcaagta
     3901 gcaggagtca gtttcgcaat gggaaatgca gaaatcgaag taaaagatta tgccaaatat
     3961 acaacggaca caaaccttga atctggcgta ggaaaagcca tattgcgcgc gattgcagaa
     4021 gacttgtagt tttatctaga aaagagggat atagatgtca gaatttttca ttcaggaaaa
     4081 gcagcttgga cgtgcaactc ggacaattgt caaaaacgaa aaaggacatt ccctgttttt
     4141 gatggttggt cgctggggaa caaagggcga tgcattgtct ctttatgcca tgaatggcga
     4201 tctcgttgct cacatcaagc agataagcct gacattcggt acgcgttttg agctgtataa
     4261 agagttcgaa aaggtgggaa ccatgcagaa aatattcaat tggcccggtg acttttatta
     4321 tattcgtaga cttcactgga cagtccaagg tgatatctat aatcatcaat atcaaatcca
     4381 tcatttcaat cagcaaatca tgaaaatgga taaagcaaca cttttttcag gagattacta
     4441 tgtattggat gtctcaaatg atgaagatgc tccagtttgt atttgtattg ctgcggtttt
     4501 agattactgg ctctaccgta aagacaaaag tcgcgataag cctgtttctt accgacttaa
     4561 cttaagttaa aaagatgtac ctgaaatgcg gaaatacgca tttcaggtac atctttttag
     4621 atattctttt gcatatgatc ataaagatga ccactgatca tccgctgacc gacaactttg
     4681 aagtcatggc gttcgtataa ttttttagcc tttggatttt tttcatccac acttagaccg
     4741 atgacggagc gattagcttt cttcgccaat ttaggtaatg cttctaataa tcgcgaaccg
     4801 atgccttgtc ctctaaaatc ttctgataca ctgatagagt ccaaatacca ttcgttggga
     4861 aacgtttctt tatcaatgaa caaacgtgta gacgcatcta gctgatattt ctctaagata
     4921 ggtaccagtg gctgatcgat gattgattct tcttcatctg tataaccgta agcaactcct
     4981 gcaactttac cgtctatttc ttctacgata cctcgtgaat aactgtaacg ataatttgga
     5041 tcagtgattg cttcttctaa aatctctaaa gtcttttgtt cccctgtctg cttcaaaaat
     5101 ggcagttcca tatcttttag aatcactaag atcaatgggg cgatttctgg agcatcttct
     5161 tttctcgcaa aacgaatcat agtttcctct ccttttctcc attctaacat gcagaaaaag
     5221 aaatcgcaaa atcgttattt taaaaaatct tcaatctttt cagtaatgaa ataaccatga
     5281 gtaacgattc ttgccgcttc agttcggcaa taagggcaga cgactggcat ttctttatca
     5341 ttatgttcga ctacattatt gacttcttcg tatgcaaatt tttttccgca attagggcat
     5401 ttcatttttt ccatgttatc tcaacttcct ttcatcttta ctctcttagc ttactcttat
     5461 attgtaaaaa agagaagcaa tatccttctt ctatgcagat atccatacct gacggtcctg
     5521 ttctccatct attaatcgga ctttataata aggacgttca tctttgacta gaagtgtcca
     5581 ctctttcact tttgcttttt tcccgtagta gctgactgct tccttcatcg ctgcttgcgg
     5641 ttgtttgaac tcttccgttt tttctatatc aaaaagatgt tctgcggcct tttccgtgtc
     5701 atttcgtacg gttgttttcc cagtggatgg attgactgct atgacttgtc cttcacaaaa
     5761 aaactggtat tcatatgttg cattcgtagg attctccaaa tctttttcaa ctgcaaaatg
     5821 aatagcagat atgttatgtt cttttgtttc gttttttacg attgctgctg cttttttcaa
     5881 tgagatccga tattcctggt cactaatatc atccatgtga aaagcggcat tgagcagtcc
     5941 cgtcataccg gtaatcccta ttacgatgag ggcagcaaca ccaaacatca tttttttcat
     6001 tcttcattct cctgtttctt ttttttaaag aatatcaaga aatgaacgaa tgaaaaatcg
     6061 aatgaaacga aaaaaaagtg acaattttgt tatctttatt cgtgcagttc taatggcaat
     6121 ccgtctggat caaagaaaaa cgtcattttt tttcctgtaa aggtatcttc tctaatcggt
     6181 tctgattcga tccctaagga ctgcaattct gcaattgttt cttcaatatc gtctactttg
     6241 aaagcaagat gacgcaagcc acaagcttct ggatagtttg gtcgttgtgg cgcattagca
     6301 attccaaaaa tctccaattc agcacttcct agtttcagat ctaatttata gtcatctcgt
     6361 tctttgcggt aattttctct gattacttca aatcctaatt tattaacata aaaatcacgt
     6421 gatttctgat aatctgaaac aatgatggct atatgatgga tcgttgtaag gttcatatga
     6481 gcactcctat tcttcgttta agctcggatc tatattaatc tccagttcca ttgtaaatgt
     6541 gtcagattga aaaaaatagc tgtttttcct ttttataaga aaaaaggcaa aaaaaacaga
     6601 aagtaggtcc tccaagacac tcttcctgtt cccaatataa taaatgaaag taagaaatta
     6661 tattttcaag aaaacaaaca gaaaattatc ataacacaaa ttatttataa ttgaaagact
     6721 agtttttttt caagaaaagc atacaataga ttgtgttagt aaatacagaa cacactacat
     6781 ttagtgtctt aaggagtcct catacatgaa gtcatatttt cagtttttag ctcatcagct
     6841 aaaaggctgg ccgcaacaaa attattatct tttctttttc agtcttggct gccaggtcat
     6901 gactcttgtc aatagtccca tcacttggct gtccgttatt acatttatcg gtaccacgct
     6961 tggtgtattg tgtattttat ccatcaatgc ggcaaaatca gtcaacggta ttttaggtat
     7021 tttgtctgcg atttgtttta ttatcgttgg gttttcagca aagaactatt taagtatcgg
     7081 tgaacagatt gcttatatga tcacattgga tatccccgta ttgttaagta aagaatggaa
     7141 tcataatatg gcttcaaaaa tccgaaaatt cactggaaaa agctggttta tcgcaatcat
     7201 cagtactttc cttgtctatc ttgtttcagg attcctgatt ggtcgttata cagatgatcc
     7261 tcggccgtgg gtagatgcca ttgctttttc tattagcttg actgctggta tcatcagttt
     7321 cttgcgttac aacaaccaat attactggtg gttagcttca gggcttgctc aaatggtcct
     7381 ttggtatatt tcatttacgc atggatctgc aagtttggcg atgttcgtga acagctctgt
     7441 ttacttgctg aatgatgtcc ttgcatttac cgtttcacct tggtataacc aaaaagaacg
     7501 tgaacgtatg caaaaaatgg aagaagaata ctacaacgca caacaacacg cataaaaaaa
     7561 ctgagcgtgg gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg
     7621 cggaatcaga agcaactcct tcggaaataa gctgaaattc acaaaaattt gaaaagcaat
     7681 tttcgtgaat ttcttcttat ttcttggagt taaacgcttc tgtcccagcc tctatttttt
     7741 atagtaattc cttcagatac tttcttgcct catcattgaa acgcagtgat tcttctaact
     7801 tctgataata ttccaacgct tgtttctgag cttcagtaaa acgataatgt tttcctttcc
     7861 atttgtcgat tgtctcttgc acatttccag attggatatc tttcactact agttgccatt
     7921 tttcttttga gactgtacgg tctttcttat cagcagattg tatagcttct cgttctttac
     7981 gcaatacttt taaaaaacca tttctgacta tccgaggaat gctgtcgaag aacccttctg
     8041 taaaaccata gcgacaatat tgcttccaaa taaatagata gatttttggt tggatgaatc
     8101 gttggttttt cgcaaaatat tccatctgag cagtgacttc tggtgttcgt tgacgttttg
     8161 tctttctcaa cacggaaata aatgtttcaa aataagcatt aggatgtact ggcacttctt
     8221 catatacgat ggattgccaa tctttacgta aaaatgcgcg atagttgatc aagaattctt
     8281 ctacatcctt tactaattct tctgcaaagt cgtcaaatag ttcttttttt atctgtcgct
     8341 tttttgactg cccattgatg tgttcactta ttttcttgat ttcattttcc aatgcttgat
     8401 aatccgctat gtaaatcggc atttctactt ggcgaaattc tgcgagccgc tgagctaatt
     8461 ttatatccgg taaatatggc tgtttcatcc gacgattttg gtacaagaca aaacagtatt
     8521 tttgccatag tccttcagga aaatcgatat tcttctgctt aagccataat aattcgtcta
     8581 aggccagatc aacttttgtc attccctgat ggatcgaagc tgccactgtc ggagagacat
     8641 gcaggtgatc ggcaaagtgg ttgatgccta gttttaggat ctttccggtt tttggcgatt
     8701 taacgatgta ttgcactttt aattctctac cacactcaca atacaatttt tgctggcttc
     8761 gttttgttcg aaaaaacgga ttgatcttga agtcagaaaa cacccaatct gttgcagcta
     8821 aataatgttc ttccataaaa cgagagctga ttagatatcg gcggtgctta tcgactagtt
     8881 cccgctgttt tttcgttaat tttgcataga tctcccaacg ttgactgctt gtgtattgtt
     8941 tagaaatttt gatgggatag cttttgctga tttcttttgg aaaccattct tcaaagacgg
     9001 attgccattc ttccacggct gtttctttcc tttctaatta caaaaagctg gctactaaca
     9061 taacgttagc aaccagccag tgtttcttgc taaattagtg agttgttccg gcatttccgg
     9121 aaatatcttc tttattttct actgctgctt caactgcata agtcaaagaa gaagcaatgt
     9181 cttctaaaga catagaaggc gtacccactg gacgatcgat cacttgatcg ggtgagaatg
     9241 acacgtggat gaacccacct cgaattgccg ggaattgtgt ttcgatcaaa tgaagcaaac
     9301 gatacatgat atcattgcag acaaatgttc ctgctgtata agagatgtac gctggtagac
     9361 catgatcttg tacatttttt gtcattgctt tgattggcaa tgaggtaaag taagcggctg
     9421 gaccgttttc ttctaaagcc gtatcgaatg gctgatttcc ttcattatca gggattcttg
     9481 cttctgctag attgattgcg acacgttcga acgagactgc tgaccgccca cctgcttgtc
     9541 cgacacagat cacgacatct ggttggtgct gttggatggc ttctttgact acttgactgc
     9601 ttttttcgaa tacggtcggt acttccactt tgatgatttc agcaccgctg atttcatctg
     9661 gcattttttt tactgcttca taggctggat tcactttgtc cccaccaaat ggatcaaaac
     9721 ctgttactag tactttcatc tgttgttcct ccgtccgatt gatttaacat atggttatct
     9781 tttatcatac cattttcctt gaacagcgta aataaaggaa acaatagttt aaataacata
     9841 acagatcaaa cttaatatat tcttataaaa aaatagtgtt ccactacaat gttagaagag
     9901 gttgcgacaa ttattctgtc acaacccctc tcttctatta tttaaataga atcatataag
     9961 caatttggaa aactagcata aataacgcaa taaacaactg gtttttgatc actccgtatt
    10021 tgtctttcat ctccagcatc gctacaggca cgatattgaa gttagcagcc atcggtgtca
    10081 gcagggtccc acaaaaaccg caagtcaatg caaccattcc tattaacgct ggattcgctc
    10141 catgactaaa tacgaatggt gcaccgatcc caaccgtcat cacggtgatg gccgcaaatg
    10201 cattccccat gattaccgta aatatcacca tgccaagagc gtaaatgatg atgccgatgt
    10261 tcacatttcc ttctggaacg atcgtaccta ctgcattgga aatcactgtc ccaactccag
    10321 cgcttgtaaa taccgcacct agagaagcta aaagcattgg caacatgctt aacggcccta
    10381 ctgtgcccag catatctgct gcatcatcta aaaatgtact tggtcgatta tctcgagaaa
    10441 agaacatcaa gattagcatg gctgcaaaaa caccgacgcc cacgccgact aaagcaccta
    10501 aattggtaaa tagagcaaac aagatcgcaa agacaccgat actcaaagct ggaataaaaa
    10561 ttttcattcc tagtttgtct gacatttttt ccatatattc ttttgatgga agccggcttt
    10621 ctcctttgct gactttctta gcaattgctg gtatggtcat cgcaaagatc aagccaccgt
    10681 tcacgattgc cggaagccat cttcctcctg caataactac ccctaagacc gtccagaaaa
    10741 aggctgaccc ataacgatga ggattggttt tatctgttaa atttcttact ccagtgtaaa
    10801 tcaatacgag tcccatcacg atataaagga tctccagtaa tttatcgcct aataaaatat
    10861 caggatttgt aaaaaagtcc atcttattgc ccctttcctg gataatattt cttcgctaat
    10921 ttcttttctt taagatagaa gtagacactt gccacaacta aagccgtgat agccactgga
    10981 acttccacta aagcaagctg tcccaatgtt acgtcatatc ctagatcttt cagtgttgat
    11041 tggactagca acgcccctgc tcctccgatg aatagtactt ggccaaagaa ccagcagaca
    11101 ttttccatag ctgaagccat accttttagt tcttcttcat gcttaggatg gacttctaac
    11161 ccttttgatt cgatggtccc caatgccatc ggtaaaatga ttggacgaac aaatccagca
    11221 actccaccaa aactcacgtt aaaagccgca aagatcattc gaaagatccc ataaatatct
    11281 acaatcatac cagcagatac tttcttgaat cgcttgatca aggaagccgc tgattctttc
    11341 agaccatttc tttcaagcgt tcctgttgcc aacataatga tgataaaaat cgccatcccg
    11401 cgattgttta caaaacttgt acctaaaagt tctaacattc cttgaatgcc aagaccgcca
    11461 gtcaaagctg taacaactaa agcaaccatt acgatcaata tcgagtctaa tttaaatgca
    11521 aacccgagaa taacaatcaa gacgcctaaa aggctaagat aattttccat atattttcct
    11581 ccataaaatg acaatatttt tttaattata aagcaaaaca cataaaaaat atagttgttt
    11641 tctcacgaaa tagacactat ttttaaatat ttttcgttat ttttttatca caaaaattat
    11701 cttacttatt gtaaggtaaa gcttttttta atttattgcc cgataatata tagaaataag
    11761 gtctagttac tgtttttttt aaatgattaa taaataacaa cttctttcta tgaaattgca
    11821 aagcattcat cttcttttcc gctaacgata tatccgtcag tgcttggtac actttactaa
    11881 aaagtatgag attgccgata tctggatagg tgtgctatac tacagaaaaa gatatacaat
    11941 ctgccaccgg gtaggataaa gaggatgatc attccattag gtctttgaag ggatgatttt
    12001 cctctttttg tgtatttttt tatagttaag gaggaatgta agtatggcat ggatggaatt
    12061 aatcatagca ggaattttgg aagtattctg gtcaacgatg atgaaatgga gtgatggttt
    12121 ttctaaaatc aattattctt tttacacagt gattggcatg attgctagtt tttacttttt
    12181 gtcaaaggca atcaaaagtt tgccgatgag tctctcttat cctatctgga cagggattgg
    12241 cgcagttggt tcggtcataa ttggtgtggt cttgtttcat gataagctga atctttccac
    12301 ttggttcttt gtggggatgc tactcatcag tatcattggc atcaagatta ctagcggcca
    12361 ctagacactt ggaataagta ctgatcagaa aaaataagcc tttttattta atattcaatt
    12421 tttttatgaa aaaataagaa ataagtccaa tgacctagcg acttctccac tcatcaggtg
    12481 tatgcttgta ctatctatag aaaaggagtg aatatttttg aaaagtcttt cggtccaaga
    12541 attatccaag aactttggtc acaaacaagc cctcagcgat gtcagtttta gcttatcccc
    12601 tggcaaaatc gttggattag taggtcctaa tggcgctgga aaaacaacga tcatgaaagc
    12661 tattttagga ctgatccatt actcagatgg aacaatctgt attggtgaaa atgagatttc
    12721 tccctctagt cataaagggt tggaacaagt aggagcattg atcgaatacc cgggtatcta
    12781 tccattctta acaggctatg atcatctgaa gttattctct gaaaccaacg atgtatctgc
    12841 tatcgacact atcgtgaatc agctcaagat ggaaaagtac attcacaaaa aagcgaaatc
    12901 ctactcgttg ggaatgaaac aaaaattggg aattgctctt gccttactga accatccgtc
    12961 tatcgttatt ttagatgagc cgatgaacgg tttggatcca caagcaacaa gagatgtgcg
    13021 aaaaatcatt accgacttgg ctactcaggg cacctctttc ttgatctcca gtcacttact
    13081 cagcgagctt gaaaaaatgg tcgatgagct gatcctgatc aatcaaggaa agatcatcaa
    13141 acaatgtaca atggctgaat tgaaccaaac agcgcaagat ttcatcgtta tctctacaac
    13201 aaatgatcta gctgctcgaa cgatcttgac agaagctgga tatactttgg aagatcctga
    13261 taaaatcaag cttttaaaag aaaacgaaga attattagga accgtgatcc agctattgtc
    13321 tgcacaaaat atccatgtga cagacgtcca gcatctgacg aatgatttgg aatcttctct
    13381 attggaaatt ctggattcag taaaggggga agaagaaaaa tgacattgct tgtaaaacaa
    13441 gaattattta aattgatcaa gaaaaagtca actgctgttt tatctgttct attagttgtt
    13501 ttgctcatag gaacagcact tctagccaaa aaatacacga cgatcattga tccagttgaa
    13561 atgactgctc agctttttag tgccacatct tggattgtct ttatcatgat tgcagctgca
    13621 agcacgatca tttcgatgga agcacaatat ggtacattga aaaatctcct ttatcgaaag
    13681 tattctcgag gagaaatatt agtaagcaaa tggatcacat tagttattta ttctgtttat
    13741 ttataccttt tagctattat tgtcactgtg ttgatgaaac tgattctttt cccttcgatt
    13801 tccttcacgg agaaggtttc tactggtcaa acactgatcc aaagcttgtt cacttatact
    13861 ttgggtagtt atatcggttt atggctgatt ttaagtttag tgttgatgat tgcttgcttt
    13921 atcaatagtt ccggcgcatc catctcagca ggaatcgtct tttattttgc ttcgtcaatc
    13981 atatctggta tccttattgc actcatccaa aaatgggagt ggatcaaatg gaatccgatc
    14041 agtatgttga atctgcaaaa ccaaattgga aacgaagaga tcatgaagca attgactcat
    14101 ctttctacta atcaaatgct attcggtaat ttagcttaca tcgttttgtt cttagcatta
    14161 ggatatctcg tatttaagcg aaaaaatgta tgatgactca tcaaaaaaga ggctgtgaaa
    14221 gagacgttca gccttattgc cgattggctg tctcttaaac accgtttatt ctgaaaaagg
    14281 agctgtgaca acatttgtgt cacagctcct ttcctagtat gtttttgaat aaacgacagg
    14341 ctcacaacct ctctttatac ttcttttata cttctatacg tgaagcatcc cagtggcaaa
    14401 taaaaccatt gcaatcactc cgaccatcgt cacacctata agtaaaatct tatctaagtg
    14461 gatatgttgt acaggctcgt tttgctcctt acgtgctttg aggtaaaaat aaatgcctgg
    14521 caagaaaaga acaagaacaa gcattacttc attaaaggct acgactagca tagcccatac
    14581 ttgaaaggca cttgctaaaa gccctactaa aaattggacc cattctttcc gctgataaga
    14641 aagtttcatt tggtacaatc ctaagaaaag ccacgtgaag aaaatagctg ctgtaccaag
    14701 tgtgtaagca aagttataag cactgtcagt gaataataat gaaagtaaaa agacattcgt
    14761 acataacgtc gtcaatacga tagcataagt tggtgcccca tttttgttga gttttcccca
    14821 tgctttaggc aaaagtccgt cttcagccat gtttcccatt gtttcaatcg gtaagatcgt
    14881 ccatgagagc caaacaccaa ctaaagaaat gatcaaaccg ccgttgatca cgactgctcc
    14941 ccatttacca accatcattt caaacacata gcccatagca ggctgcgcct tgatcgtttg
    15001 cagctgattc atcgtcattg tcccatatgg aagcatggaa accaagacgt aaataataat
    15061 caagccgaat atccctagaa tcgttgcttt cccaacatct gattgttttt ttgctcgtga
    15121 acctacgaca gaggctcctt caataccaac aaatacccaa agcatcacca tgatgcaact
    15181 caatacttga ctggaaaacg ggatcggctg tccattcgca ttcaatataa tgtcgctaga
    15241 aaagttatca atgaattgtt ggatcgaaaa cgtgctgata gctactacaa taaataaaaa
    15301 taatggcact aatttacaga atgtgacgat agcattgacg aaactagcat tttcgactcc
    15361 ccgattcact agccatgcat atccccataa aaagactgta ctcatcacga tcgatgccag
    15421 attttgccca ccttcaaata ctgggaagaa atatcctaat gcgctcatca acaatgttgc
    15481 aaaagctacg ttgcctaacc agcatgtgag ccagtaaccc caaccgctga taaaaccagc
    15541 gaaacggcca aaaccttctt ctgcgtaact aaagatcccg ccttttaaat ctggacgttt
    15601 tttattgata ttattgatcg acaaggagag catcaacatt gctgttccta cgagtaccca
    15661 actgaaaata gctgggccgc cagccgatcc ggagatgtcc gtcataatac caaatacgcc
    15721 acctccaatc gctgaaccaa ttactaaggc agcaagtgga attaaaccaa ttcctttttt
    15781 ctgttgctct gtatccatgt aagcatcctc caaaataaaa ccgcttttat caaaaagtat
    15841 aacaaacctt ttttattttt acttaattaa taaaaacttt aatagaaata taattctacc
    15901 ggcttttttc tcattataca agcaaagcgc ctagtccctt tccaaattac accaccaaaa
    15961 aacttaaaaa agatgctata ctaaaaggaa agaattggag gatgatccat gaacatttta
    16021 tatatatcga tttcaggaaa tacgcgtgcc tttgccaaac atctagcaga atatgctgaa
    16081 aaaatgcatg cagaagatcc tgtcaatcct gaagttactt taaaagaaat ccatgaaaat
    16141 agtgatttta cgaaagaaac agaaccattt tttactttcg ttcctactta tttagacggc
    16201 ggaaatggtt tagataacgg tgatacagaa atcttgaccg aaacgatgcg tgaatatctg
    16261 gaacatgaag acaatcataa actttgtctt ggtgtagtcg gtagcggaaa taaaaacttc
    16321 aataatcagt attgtctaac agccaaacaa tatgcacaaa catttggctt tcctttcttg
    16381 gcagattatg aattacgggg aacaccatct gatgtgacac gaatttatca gattttagca
    16441 gaaaacaata aataataaaa aagaagaagc tgaaaaatag atttcagttt cttctttcgt
    16501 ttctattgtt tctatcatat taagctttga catccatcac aggtgctttt gcttttgctt
    16561 tacctgtttt ttccaagaca aagtttttta cacgatccat attggttacc aagacgatca
    16621 tggatgtttc tttgcctgct gctttgatcg cttctaagtc agcttctgct acaagcgtat
    16681 caggtgtcac ttgctgccct tctgttactt tcacatcaaa tggcgtaccg cctaagtcaa
    16741 ctgtgttgat tcccatatga agcaagattt ctaaaccatt tggcgttgtg atcccaatag
    16801 cgtgtttcgt tgggaagaca gagctgacta ccccagtaac tggagaatag atatgtccgt
    16861 tttctggttc tacaaagtag ccttcaccca tcatcttttg tgcaaaaact ggatcacttg
    16921 ctttatctaa agaaacaact gttccgtcag caactgatga gaggatagtc acactttcag
    16981 ctgaagtttg aggtactact ttttcaggca cttcatttac tgtggctgtt tcagtcgcta
    17041 attcttcttt ttctaaacgg tctttaggaa taccgaagaa gaatgtagca acgaagccac
    17101 cagcataagc tgccaataat ccaaggatgt agcctaacca tttaccgtca gagatcaatg
    17161 ggatcaaagc ggctcctgaa ggtccaatag cgatagcccc gacatttccg atcattccga
    17221 tcactgcccc gccgatacct ccaccgatac aagcagtaat aaatgggcgt cctagaggta
    17281 atgttactcc gtaaatcaat ggttcgccga ttcctaggat cccgactggt aaagctcctt
    17341 tgatcatttc tgttaattct ttatcttttt tgcagcgaat ccataatgcc aatgctgcac
    17401 ccacttgtcc tgcaccagcc atcgcaagaa ttggaagcaa tagcgtacga ccagtttcat
    17461 caatcatttg cacatggatt ggagtcaaaa tttgatgcag tccgaacata accatcggca
    17521 agaataatgt gcctagaacg aagccggcaa atgctccacc aacgccaagt acccagttga
    17581 tcccaccgac catgccatca gaaatgaaac ctgctaaagg catgatgaag aaaatttcaa
    17641 tcagaccgat ggcaatcaat gaaagagttg gtgtaacgat gatatctacc gcatcaggta
    17701 ctactttatg cagttttttc tcaacgattg acaatagcca aacagcgaag atgacaccta
    17761 ggatcccacc ttgtccagct gcaagagctt caccagtaaa taagtttttc agtggtgctt
    17821 ccggattcat ccctgtcagc atcacaacgg ccccgataac tccaccaaga gttggtgttg
    17881 caccaaaaac ttgtgcagca ttgatacctg tatagataac taggtagcta aacatcccgt
    17941 ttttcaagat gttcatcaca tcgatatatt gttgccatgt tgctgcatca agtgttccgg
    18001 ctgtgactaa gtttgctaaa actgcggcaa tccctgccac gatcccagta ccgacaaatg
    18061 caggaatcat tggcacaaag atattagaaa tgtcttttaa taccgatttg attttagatg
    18121 gtttttgttt cgatttttgt gccgctttca tttcttgtgc ccgttcgttt acacggtctt
    18181 ttccagatgc tgatgaattt gctgtagctg tacttacaga agaaagtttt tctcctaatt
    18241 tcacgcctgc caaatcaacc atttgctgtg ctactttgtt cactttccca ggtccgatga
    18301 tgacttgaag ctgttcatct tctactacac caagcactcc tggaatcgct tttaattcag
    18361 agacatttac aagatcatca ttgatgatct tcatacgcac acgagtcatg cagtggacaa
    18421 tgctttctac attgcccatg ccgcctgccc ctttataaat ttgttttgcc agttcttcca
    18481 aagtcagttc gttttttgcc atggtctttt ccccctcata aggtcccttt tacaaatccg
    18541 tttgcttcag ctaattttgc ttgagcttcc tctttatcac tatttgtcaa aatcatgacg
    18601 atagccagct taacattttg atctgcttct tcaaagtact tttcagctgt ttcgtaatca
    18661 gcttctgtcg cttgcatgat gattcgtttt gagcgttcga ttagtttttc atttgttggt
    18721 ttgacatcga ccatcaagtt attgaatact ttacctatac caatcatact gatagtagaa
    18781 atcatattca agataagttt ttgtgctgtt cctgacttca atcgagtaga gcctgtcaaa
    18841 aattctggac ctgcatcgac ttcgattggg aaatccgcgt gtttactgat ttcggcttgt
    18901 ttattgcaag aaatcgttcc tgtgacagca ccgatcgatt ttgcgtaatc taatccaccg
    18961 atcacatatg gtgtacgacc gctagctgcg ataccaataa ctatatcatt ttttgtcaaa
    19021 ttcagcttga ttagatcttc ttgccctagc tcataagaat cttcggcccc ttcgacagct
    19081 acagtcattg cttcttctcc accagcaatt agacctatga ccatttccgg ttctacaccg
    19141 aatgtcggta cgcattcagc agcatctaac acacctagtc ttccacttgt tccggctccc
    19201 atataaatca gacgtccacc ttttttaaag gactcaatcg tgttagcaat cactggttcg
    19261 atacttgtca gttctttttc aaccgcagcg gccactttct gatcttcttt gttcattagt
    19321 tgaacggctt gagaaatact catctcatct aatccaaaag tttcttggtt tctgcgttcc
    19381 gttgttaggt tctctaaatt gattttgctc atttttatct tccttctttt cttataaatt
    19441 gatagttcat accagctttg atttggttga tcaatggtag ctcttctttg ataactttag
    19501 ctactacatt cactttttca tccgctggca aatcatattt ggttaattgg atctcaccca
    19561 tatagcgcaa atacttttcg ttatctaaag taatagaccc tttcatacgc gtagctgtat
    19621 ttcttgctgg gataggcgga atctctcgaa aacgtgcatc tacgcttcgt atgacatctc
    19681 ttgcatcgtc ttgccgattc gtatgcttcc ctaatatata ttcgtaaaat tcttcatcta
    19741 caggttccgt atggagcaag actgtctgtt ctttttggaa agaagcaaat tgctcctgaa
    19801 cgttttctga cagacctgca tctccgatgt aaacaatatc cgtgttactt gctaatagat
    19861 ctagagcgcc tgcaagagga tggatccctc gatgttcttc caaagttggc aatccttgat
    19921 atagtgggcc tctcaactct gtatctcctg gtacaaaacc ttggacaagg agaccttgaa
    19981 ttttgagcca ttgatttttt ttctgatacc attctttgtc cagccctgtt tcgggacgtg
    20041 gataataatt atgccaagct tcaatctgtg agaaatcagc ttctgcttct ttcaattcat
    20101 cgatatcttt aggcgtgatc gtgctagcat tcaaactgat tttcaattga tgtgaccagt
    20161 cggcaatctg ctgattggaa atatgataat ccattcttaa acctgttaca ccaattgctt
    20221 tcaaaggcgt tagatcttct aatgaaaaac ccgctcgttc caatgcctct cctgaaatat
    20281 cgaccatcaa ttctagttta ttctttttcg cttcgcttcc tagttcgatc aatcgctttt
    20341 gatatgccga aacatcatct tctggtatat gcatggaagt gaaaataccg acaaagccat
    20401 tggatgccat ttggcggata tactgtttct tctcttctga caaagaatcg ttcataaata
    20461 ttgaaaagcc aaacatatta tccctctttt tctgtatccg tcttcatttc tatgactaga
    20521 gtatatatct aaagaaacaa aatttcaata cttttattaa aataattaaa tttcatttct
    20581 aaaggttgag tttatattat caaaagaatt gtcataacct aaaaaaggca ctgctaagca
    20641 aaagcagtgc cttgaaatat tttatttaaa taacacaaaa acataattac tcataagaaa
    20701 ccaattctgt attatctacc atgaatgtaa agaacccctc attaggcatc ggatgaaaat
    20761 aagcattgat cactcttatc aaggcattat ggcaaactaa cagatagaca ttttcttcgt
    20821 cttcgatgca ttctttcaat aaaggaacga tccgtgcata cacatctaag actgactccc
    20881 catttgggaa tcgtacagcg aacgctaaac gtgctttttg aaagttctca tctttgcttg
    20941 gcagtccatc ataatcccca aaatccattt ctaccaatcg ttcatcaacg gtcagcggca
    21001 agctgagacg atcagcaact gcttgtgctg tatctcttgc tcgttgaaga ggggaatgga
    21061 tgattttcgt gattggaaca ggcaaatcta ctaccttttc tgctagtttc tgtgcctgca
    21121 tctgtccttt ttcagtcagc ggcacatctg cccgtccgca aactaatcct tgtgcgttcc
    21181 aagttgtttc tccgtgtctc gttacataaa gcatgtgatt ctctccttcg ttggcaaagt
    21241 tccccttaac cataccttgt tgttcttgag attacaaggg ttagattctc tcaagagaaa
    21301 taatctcagt agatacttta tccaggaagg tttgatcatg ttccactagt aacatggcag
    21361 gtttcacgtt caagatcagt tgttctaatt gttcttgatt gaagacatcc aaataattca
    21421 atggttcatc ccatgtatat agttcagctg gctgtgataa agatttagcc aattccactt
    21481 ttttccgttg gcccatactc atctgctcga tcttgttatg aaaaacatct ctttccatcc
    21541 caagctttcg gaggttgttc aaaaatgctt ggtagtctac ttggtttttc tctgcaaatt
    21601 ccgccaacgt tcctcgattg tcttcataat tttggcttgc ataactaatg ctcagatgtt
    21661 ttggctggta tttttctcct ataaccttgc cgttgaatgc ccccagaaga taatggataa
    21721 tggatgactt tcctgcaccg ttaggacctg aaatcgccac ccgctgatga ggctcgattg
    21781 taaaatgaat tggctcgaat aacagatttt cataccctaa ttgaagatct tctacgctta
    21841 aaagtcgctt atggtgagac gcttggctat tcatcgtcaa agaatcgata tattcgatat
    21901 cttttaatag tttttctttc tcgctgatct gggtctccat ccgattcacg attgctttgg
    21961 atcgtttcat cgtccgtgca gcatcagcac ccactgctcc tttattcact cgtctagatt
    22021 cagtatcgat gaatccgact tgtttctttg ttttatctcc ttctcgggaa cgagaccatt
    22081 cggctttttc agctgctgtt ttctttagcc tactgacttc ttttttcaat ttttcatttt
    22141 gagccatttc aaattcatca cgaagttttt tctgttcttc atagatagag aaattccctt
    22201 gataaagttc cagttgactt ttttcgattg ctaaaacatg gtccactact tcatcgataa
    22261 atccccggtc atggctgacc acgatgaagc cttgtttttt ctttttcaaa taagccgcta
    22321 cttgttttct accagagata tccaaatgat tcgttggttc atcgattaac gggaaatgag
    22381 tgtcatccac aaataaaagt gccagtagga cttttgtctt ctctcccccc gatagtgtgc
    22441 tgaattctct ccataagatt tcaggatctg tttgcatcaa ttggagctct ctttcgattt
    22501 cccatatctc aaaatccgta atatcattta acacgtaata ggttaaacgt tctttatctt
    22561 ttgtctgttg cgggaaatag gcaaattctt gctgatggat tacttgccct tgataaggta
    22621 gtttgttttg tagaatattc agtaaagttg tctttcctcg accgtttcgt ccgataagtc
    22681 ctagtttcca ttgtgtgtca aaatttagat ttgcttgttc aaataataat gtgccttggc
    22741 tgtcgtagcc gaatgtcaga ttttttattt cgattttaga cattttccat cacctcgtaa
    22801 aaaaagacca gtcctatttt cagaactggc taaagaggat gcaagggttg gatacacaca
    22861 aaaagaccca tacatcgaaa aaaccaagcc aatcccgaaa atctgcacac tgcttccttg
    22921 tctccacaag gtacagtctg aatgcagctt tcatcaatcg acttaatttt tcaatgcacg
    22981 gatccctcac tttgtcctat atttgaaatt acgatagcat aaagaaaatg attttgtcaa
    23041 ctacttcggg aggatggtga aaaatctgct aagtgtctgt cctaagattt ttgttttttc
    23101 taatgtttgc ctgtgttcca ttgcttcttc taaacctatt ggacccaaat gaatacttaa
    23161 gacaattggg agaaaatcag aaagtccttc tagctcttgg tctctacttc cgcaaatagc
    23221 gattattggt tttcgatatt tttttgcaag cactgctaca ccgtatggaa cttttccgtg
    23281 gatcgtttgc tgatcgatcc gcccttctcc tgtaatgatg acatctgctt gttcgatctt
    23341 tttttccaat ccagtgattt ggctgactaa atcgaaacca gaaaccatct tcgctcccaa
    23401 caaaaaaagg gctccaccaa taccgcctgc tgctccactt cctggaatat catctaaatc
    23461 cacctcccag aaatctttta tttgctgagc aatctttcct gcttgtttat ctaagaaagc
    23521 tagcgtcgta tctattcctc ctttttgtct tccaaattgc caagcagctc cttgttgacc
    23581 tgtatatgga ttggtaacat ctgcagcgat aatcaatttt actttagaaa caagaacgtt
    23641 ttttcgatca aaatgaagtt ttttagtaga taataaagga tttccttctg tgtacccttc
    23701 taaagaagca cccaaggatt ggagtaatcc tagaccacca tcgctacacc cgcttcctcc
    23761 taaagtgaca ataatttgtt ctacttcttg aagcagtgca tctttaataa gttctcctaa
    23821 ggcataactg gaagcaagtc ggatagtttg atccgttggt ttatcaagta gatctaaccc
    23881 caatgcttgt gcagattcga tgactgcggt gttttttcct tgccaagttg tcaataaata
    23941 gctgattttt cgctgacgga ataagagatc catcccatca atggtacgcc atgttcccct
    24001 gagcgcgtaa tagattgctt ctgcagtatt ttctccgcca tctgcaatcg gcacattgat
    24061 ttttcttcct ttacgatctt cccagccatt tagaactgcc tgatttagtt caaatgaaga
    24121 agcacttcct ttaaatgaat caattgccgc caaaatatcc atctgtatcc ctcactttct
    24181 tcttaagtaa gacacttttt ttcatttttt tcaaacaaaa caatgctatt ttcttccatt
    24241 tttctatata ctaaaaggga actcattgaa taaaggagag aaaaaacatg aaaatcgcaa
    24301 tagctggggc cggagcaatg ggcagccgaa tcggattgat gctacatcaa agcggaaatg
    24361 aggtcctctt gatcgatcgt tggcctgctc acattgaagc cattcgtaca aatgggttga
    24421 ttgctgactt taacggtaaa gaagtggtcg caaaactgcc aatctattca ccagaagaaa
    24481 tcatcgaaag caacgaacac gtggacttga tcgtagcatt aacaaaagcc aatcaattag
    24541 atgatatgtt ttgttcgatc caatccatta ttactgacaa tacttatgtt ttgtgtttac
    24601 taaatggatt aggtcatgaa gatgtactgg aaaaatatgt gccaaagaaa aatatcttat
    24661 ttgggatcac gatgtggaca gctggtctag ctggtcccgg aaaagtcaca ttgttaggtg
    24721 atggagaaat cgaactagag aacttggaac cagaaggcga agcatttaca aaaaaagttg
    24781 tagaagtctt tcaagaagca aatttaaatc cgatctatag ccataacgtt cgttattcca
    24841 tttggcgaaa agcttgtgta aatggaacat tgaatggttt atgcacgatt ttggattgta
    24901 atattgctga actaggcgcg caaaaagcgg cagagagcat ggttcgaaca atcgtatctg
    24961 aatttgcttc tatcgcagca aaagaaggaa tcatactaga tcaagaagaa gtgtaccaac
    25021 atattgcttc tacttatgat cctgacaaca tcgggttgca ttacccatct atgtaccaag
    25081 acctgatcaa aaaccatcgt ttgactgaaa tcgactacat caatggtgcc atttggcgaa
    25141 aaggacaaaa atatgatatt gctacaccat attgtgcctt tttgacccaa ttagtccatg
    25201 caaaagaagg aatccttggc gcagaataaa aggctctctg atgagaatca gctgaatcag
    25261 cagctggttc tcttttttta agcccaataa aagatcaaaa agaaaacaca actctcatga
    25321 tgccataaga attgtgcttg tttgtttcag tacataatct cttcaaaatg taggtttttt
    25381 atttgttttc aacagaggag aggtcatatt cgtattgctc ataaatagtg gaatcgttag
    25441 tgataaatcg ttttgcccaa ttttctgcat cataaaattc caatagctct gataggattg
    25501 gcttaaatgg tgtatgcaga aattccggta attcatctaa atcacgttct cgaagcaaaa
    25561 aaatattttc aggacgataa aaatgataat gagcaatcgc ttgattatcc gtcactagtt
    25621 tcttttgaaa atacatggat tcaaatgtcc gcaaagtcac tccttgctga ccctcccgca
    25681 acagttccag aatcccgcgc gattcccctg tttttttcag gtactcttca taagtaagat
    25741 atccaggttg ttcattccct cgatgtggaa gaatgaaata gttggtcgta acatctcttt
    25801 ttataaattc ctgcttatag ctttctgctc gttcttttct cccattattc gttgcaccaa
    25861 aaaatagatc cgtctttggt tcgctagaag aaaaattcat atgtttcgaa taaaatgaac
    25921 tattatgttt cagcccaagt tcttttgctt ccaaaaaatc aaaatgataa atctcatcga
    25981 ttgccggttc atcttgtgcc tgcttcagca tcgcctcgtg ttctgctttg aagtgattcc
    26041 aaaaaaataa cacaattttt gcattggtaa atgggcgcac gcgtcgagcc atctccatat
    26101 caaatccata atctaaaaaa acgatcagct gataatttgc caactgttgt ctagctgatt
    26161 taatgaattc cgactgtagt aaatcttttc cggaccaaac atctaaagag cggatggaag
    26221 gcaaaaaata aagtgattta ttttttgcaa ctagcagtac gtcatttttg ttcatctttt
    26281 ccgcctcact ttctctgtca ctttttccta tgatagcctg tttaaatagt aaaaagccag
    26341 taaaacgttt cctatatttg tatgaaaatt taacatttgt atgaaaatat gatgaaataa
    26401 atcattcttc tattaaaatt cgcctaaaag ttttctgaat agttgtaatt gtctgaaaat
    26461 ttgttatcat tagtcattgt taaatattgc tatttattct taatataaat tttgattgtg
    26521 aatttaaaag aaagaggtca tttatggaag ataaaaaatt gaaaaaacaa cttagctcca
    26581 gacatatcac catgttagct ttaggtggag caatcggtgc aggtctattt aaaggaagtg
    26641 gcgaagcgat tgggatcgcc ggcccttctg tattgattgc ttttttgatt ggtgggctga
    26701 ttttatatat cgtgatgaaa ggtctgggaa aaatcgtttt aagcggtggc gacacgcatc
    26761 acggattatc tggtttgata cgcccttatc taggtgctca ttctgctgat tttactgatt
    26821 gggtctactg gtctatgtgg attatcaata tcatcgctga agctgttgca gctgcttcct
    26881 ttttgcagtt gtggttcccg cacattccag cctgggtatt tgtttttatg ttggctgttt
    26941 taactacgat catcaatctt tattcggtac ggttattcgc agaaacagaa tattggctgg
    27001 cttttgctaa aatcagtgtc attatcttac tgatcatttt tgctacttat cttgtcggtc
    27061 agcaaatgtt gggcacaggc gttttcccta cccttcagca attgactgac catggtgggt
    27121 ttactcctca tggtatgaag ggaatcatca gttctttgct tgtagttatc tattcttacg
    27181 gcggttctga actgattgct atcacggtaa gcgaagcaga tgatcctaaa cgtgcgattc
    27241 ctaaagcaat caaaggtgtc atgggaagaa tcatttcctt ttacatcatt cctttgtttt
    27301 tgcttttgat tctttttcct tggaatactt tagcaggcac gaatgtcagt ccttttgtga
    27361 tggtatttga aaaaatgaat atcccatttg cagcggatat cgtcaatttt gtgattgtcc
    27421 ttgctttgtt ctcatctatc aattcgggtg tgtatgcatc ttcaagaatt ttgttcttcc
    27481 gtttgaagga tcgaaaaggt tcctctagaa aactagctgt tttaaacaag catcaagtac
    27541 cgcaacgtgc tgttttattt tgtaccagtg ttctttatct tggtgtagct ctttcttatt
    27601 ttgtcggtga caaattattt ggctatctgg caggttccct ttcgtataca gtattattga
    27661 tctggatcat tatcagtgcg gcatcctttg tactggcact aagaaaagga acattatggg
    27721 ataaaggaat cagctttttt gcattagctg tcttaggatt gattttcctt ggtatcctgt
    27781 tcacgaactc cattggggta actgtattga ctggcctgct ttacctgttc atttatttca
    27841 gttatcaaaa aaagaatgat gcgttcgtat tgacgaatga atgaaatcga tagaaaattt
    27901 tattttattt atgtggaata gatggagcta ttgaagaaag gagttgtgac aaaagtcttg
    27961 ttgcaactcc tttttccgaa taaacagtct catgacctct tggtaaacgg tgttcaaaaa
    28021 ccctctgtgc aaaataattg attgttctcc atgctcatga catgatagaa gaaaggaatg
    28081 aaaaaaagga gtttgtttta aatgaaaaag tattggaagt ttttgttgat gatcggtgtt
    28141 tcaacggtag ttatgtttgg tatgatgtac ttgaatgtct atgcgctcga tcatatcttc
    28201 ttcagtcgta cacgagtatt catggcgttg atgatgggtg cagtcatggc agtcatcatg
    28261 ctgttgttca tgtggaaaat gtatgaaaac aaaaagataa atgttatgat cttagccgct
    28321 agtcttcttg tttttgccgg ttccttgttt atggtacgaa gccaaactac tgtcaacgac
    28381 acagcttgga tgaaggccat gattcctcat cattctatcg ctatcctggc aagtaaacga
    28441 gctgatttaa aagatcctga agtaaaaaaa ttagcggaag aaatcattca ggcacaagaa
    28501 gaagagatcg acaaaatgaa aaaaatgatc aaagaaatgg aatgaccatt atccgtccaa
    28561 ataaaaaaag aggatgttcc agaagttatc agcttcaaga aataagaaga agctacttct
    28621 ggaacgccgt gaataagggg ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc
    28681 aaacaaaaat agcttctcct atttttcgtt tggttaggct tattaccgca gatgcaagct
    28741 tttgaacacc gtttattaag aggttgtaaa atcagcgttc tgacctgagt attagagaga
    28801 caaatggaaa aatagttcct catattttgc cattttttga gctaataccg agggtcagct
    28861 gattgaacac cgtttatcga gaggttgtga aaaagtcgct tagtttcgcg tattaagaaa
    28921 aattttgtga aatggctttc cacatttttg tataaatttg gcttaatacc gagaaactgg
    28981 cttttgaata ccgtttattc gtaaaaggga ttgtgacaaa agtcttgtca caatcccttt
    29041 ttatgttttt attgttctaa ttgcgctgtc ttgcttactg cttcttgcgg tttcacaaat
    29101 ttttttgaga ttccccaacc tgtgaaggca ctgaccatcg aaaacacagg agaaagcaaa
    29161 ctgaaaaata cgaatggcaa gtaagtgagt gtaggaatac caaatgtact tgctacgaaa
    29221 cttccggcaa ttccccaagg cactaagtag ttgattactg ttccaccatc ttctagcgtt
    29281 ctacctaata cagttggatc aaatcctgct ttttcataag tttctttaaa tgcattacca
    29341 ggcagaataa cagataggaa ttgttcacca ataaaaatat ttactccgat acttgtcatc
    29401 aatgttgtaa aaatcaaatt tggtgtgttc gtcatctttc ttgatactcg ttccatcacg
    29461 gttgagatca agccaaattc gatcaacaat ccacctaatg acaaagttag aataatcaat
    29521 gacacagtag gcatcatact ttcgattcct ccgcggctaa gcagtgaatc gatttgttgg
    29581 tttccagtat ttgaaacaaa ccctgattcg atgaccccag ccaacttcgt taaggaagtt
    29641 tgtgggtttt gaataaaaat cattcctact gctacagcga tattcaacaa aatcgttaaa
    29701 attgccggca cctttttcca tgcacacagt aacattaaaa ataaaggaat cagtgaccag
    29761 attgaaatag taaagtttgc ttctagaaca tcagtcactt cacgaataga tgaaagactt
    29821 gcggctttac ttgtgtgtcc taaaatcatg aataaaaaga aagaaacaac aaatgccggg
    29881 acagttgacc acatcatatg cttgatgtgt ttgaacaaat cggcatcaac aatagctgct
    29941 gctagatttg ttgattcgga taatggagac atcttatctc caaaaacggc acctgaaaca
    30001 atagctccaa caaccagcgc tggatttaag cctagtgttg tcccaatacc gaaaaatgca
    30061 atcccaatcg ttgacataac agtaaaggca ctccctacag cgctacctac gattgcacac
    30121 acaacaaata cagaagggac aaaccactgg acactgatca atttgaaccc aatgaccatt
    30181 agagttggaa tgatccctgc ttgtatccat acagcaatca atgaaccgac taaaataaaa
    30241 ataaagattg ggatgattcc tggtttgatg ccattgatga ttccttcgtg cagccaatct
    30301 gtcgggtatc tttttatcac tgcatacaac atgaccattc cgatcgctgc taagatcgtg
    30361 atattaggtg agacctttaa tccgacaacg ccaatcgcaa tgcctaaaac aataaccatt
    30421 aatacaagtg ttgcttctaa aaaacttggt gttccttttt ttcccatgat aatctctcct
    30481 tttttagttg ttcttatgtt ctaaaaaagt taagatatcg gattactccg ccgcaattca
    30541 tccgcgattc ctcctttata aaaataaaaa gacctgtttt aaactaagga cgaatgactc
    30601 cgcggtacca ccttaattaa aaacagatct gtttttcact ttcaaacttt tattcaaatg
    30661 tatggacttt ccatgtaatt cgtcaattag gcttgatcgg ttcacagcga ccaccgactt
    30721 tctgaacaaa acacctaacc gctactgcgt gaaaagttga cttgtacgaa gtttaccttc
    30781 cataagatca tttgtcaatg aaaaagaaaa tattttttca tttttctgaa aattattttt
    30841 ataactttca cacacaagtt tattttttac tagaaagtag cgccctcttg tttttttctt
    30901 gctactattt cttgatgttc ttgcttgatt ctttctaata gctttggctc tttcaacaga
    30961 tcaagagctg tattggcgag tagttctgct gcaatcgcaa tagaagcaag gcctttgtca
    31021 cttttagcag cagctttgaa ttcttctgtg tgtccggcaa tatatgtctc agagattgct
    31081 accgtaggct ggattgttgg tatgacttgg ctgacatttc ctacatcaga agaaccgaga
    31141 cttgttcttt gttccgtttc tatttcttca tctggaacac ctgcttcttt tgcatgctgg
    31201 aagaataagt catcaaaaga aggagtaacg atgacatcat caactgcatt ttggaacaat
    31261 ccaaactcaa aagtcgtgcc cgtacttatc gcagcacctc gtacgatatt ttccactttt
    31321 tcatatactt tatctaatgt ttgccgattt cctgcacgta aatagaaacg tccagcagcg
    31381 taatctggaa tgacgttggc agctactcct ccgtctgtaa tgatcccatg gatattgaca
    31441 tcttttggca attgcaagcg tagagcgttg attccattaa acacttggat caatgcttcc
    31501 aaagcattta ctcctttttc aggtgctgct gctgcatggg aagcgacacc gtaaaatttg
    31561 atgtcgactg gatcattcgc taaagattct gtcgttttcc cataacgaaa agctggatgg
    31621 acgcataacg ctacatcgac atcctcaaaa aaaccttctc gaacaaagct gcctttagct
    31681 gatccgttct cgcccccttc ttcccctggg gtgccatata cacggatttc tccaccagtt
    31741 tcatcaatga attgcttcac tatgctggcg gctaatacgg agtaagtacc aaacaaatta
    31801 tgcccgcatg catgaccaat acctggtaaa gcatcgaatt ctgccaaaaa ggcaattgtg
    31861 ggaccgggtt tattggaaat ataacgtgca tcaaaggctg ttcggtgtcc agctacttct
    31921 ttttctaccc aaaacctttc tttttctaat tgtttaatca acgctgctga ggaataaaac
    31981 tcatagttac ttacttcagg atgttcgtgg atatctagag caagtgcttg atattctggt
    32041 aaacgatatt ggattagatc cttcaattgg tttgttgaga ttgttgttgt cataaaaaat
    32101 tcctcctatg ctagtcattt ttcttatttt ctcccgaatg tttcccatgc aggcacgctt
    32161 gatccttttg atgttttctc gattactttg actgtgtctt ctgtttgata agcgtctact
    32221 attttttgat acgttttatt gttttcatct tcttttcttg caacgatgat attcacataa
    32281 ggacgtgcat ggtcatccac tggttctaag aagatggcat cagaagcagg attaaatcca
    32341 gcatctacag ctacaccgct attgatcact gaaatgtcta catctgacaa agcacgtgct
    32401 gtttgggaag catctaattc agaaatgtct agattttttc tattctcggt aatatcagaa
    32461 acagtgggcg cttgtccttt tgccttatcc actttgatca atccagcagt ttgcaataac
    32521 agaagagctc tgccgccatt tgtaacatca ttagggattg ctacttttgc tccgtctttg
    32581 atatcagcta catccttcaa agtgttagaa taaattccca gcggtgcatt gacagtattt
    32641 ccgatcgata ctaaatcagt tccgtgttct ttattgtaat tgtctaaaaa gaattgatgt
    32701 tggaaagaat tcaaatcaat cgatccatct gctaaagcca catttggttg attgtaatct
    32761 gtaaatttca cgtattccaa attaatgttc tcctttttca aacggtcttt cacgttgtcc
    32821 catacatctg tatcttctcc aataatccct agttttacga tagtttcttt tttgtcagtt
    32881 gtatctgctt ttccttggct acaccctgcc aaccaaataa ttgctacaaa tgtaatgact
    32941 acaaaacgaa taattttttt cattttttct cctccacttt caatgtgtaa tttttttaat
    33001 cagttgatta ctgataaatt gacttaaaaa aactaagatt agaatcaata atgtagccac
    33061 aatcgttaca tctgtttgga atcgattata ccctctcgat atagcaagat tccctaatcc
    33121 gcctgcacct actgctccag ccatggtagt cagtccgatc acgttgatga tagtcaacgc
    33181 cgagacccga atgataccag gcaaaccttc taacaaatac acacgtaaaa tgataccgat
    33241 tggactggtt cccatcgctt ctgccgcttc gatcacccca ggatcaactt ctagcagtgc
    33301 gttctcgatt tgacgagcaa aaaacggaac aactccaaaa atcaatggca ctaaagctgc
    33361 tttcgcacca atcgtcgttc caactaagaa acgagtagtc aatgctaaaa gagaaagcaa
    33421 gataatgaaa gggatggaac gaatgatatt gatcagctta tcaagtattt caaatagcag
    33481 acggttagct agtaatccat tagggcgtgt cactactaac gtcacaccta agatgattcc
    33541 aatcatcccg ccaatgaatg ctgtccaaaa agacatatat agtgtttgct tcgtcgcttc
    33601 gacaaattca tccggaatca aataaacatt gggcaaatat gttttaaaga aatcagtcat
    33661 tttatcttcc tccttcgatc actttgatac gactatctaa atcgtttttt tgtaaaatat
    33721 ttatttttac accagatttg atcaaataat ccaatgcttt tttccgctgt ttggtttctc
    33781 cagctaaagt aacgatcaaa ctccctaacg gcgtttcttg taataactct acattgccat
    33841 ataaaatatt cgctgttact tgatatttgc tgtacagatg agcgattaga ggttcgttcg
    33901 tctgatttcc aatataactt aattccacca gccatttatt ggcgatctga tcggcaaaag
    33961 cagaatgggc aatgatcgtt tctaatgctt ggtcaagatg agtagctgtt cgaataaaat
    34021 ctttggtcaa ttcttcttca ggattgctga agatctggat gctgctacct ttttcaacga
    34081 ttcttccatc ttccataacc gctaccttgt tgcagatttc tttgacaact tgcatctcat
    34141 gagtgatcag gacgattgtt aaacctaact ggcgattcaa tttttttaac aatgcaagga
    34201 tctgaagggt cgttttcgga tcaagtgcac tggttgcttc atcacataat aataccttag
    34261 gttcattcgc caaagctcgt gcgattgcta ctcgttgttt ttgtcccccc gacagttgtt
    34321 taggataagc agatgctttt tcttccaaac cgacaagttc cagtagttca ttcacttttt
    34381 gacgacgttc ctgtttcgat tttccagaat actttaaaga aaaatcaacg ttgtcaaaaa
    34441 tagttcggct ttccattaga ttgaaatgct gaaagatcat accgatggcc tttctttctt
    34501 ttcgcaattc tttgggagag agctgactca gattcgtctg attgacaatg acttctcctg
    34561 ctgttggttt ctgcagcaaa ttcatcacac gaactaacgt acttttccct gcacctgagt
    34621 aaccgacgat tccataaaca tcgccttttt caatcgttaa atctacagaa tctactgcct
    34681 gtatctcttt ttctttttgt ttgaaacata cagaaacatc tcgcaattca atcattaaaa
    34741 ttcctcctta tctaaaaaaa gcgtcctcaa gaaaatgaaa atgtcatttt cttgaggacg
    34801 cttttttgcg tgttaccacc tcatgttcaa gctgtgttca cacacaactc ctcttcaagt
    34861 acgatcatac tctagcaaga tatcggtcgc agccgtcatc gccttacatc tatgctcggg
    34921 atgaattgct cgatggccat tttccaatag tcgttccttg cttcttctca gctaccgaag
    34981 ctctctgtca aaggttcttc tatcgtactt accatttcaa tgcaaataat attcaaaaaa
    35041 aaagtccttt tactaacata aactatgtca gtaaaaggac gacgtatcgc ggtaccacct
    35101 tttttcggaa gtttccttcc ctcaagccca acatctcaaa aagagagatt ggcgttcgtt
    35161 ttaacggtcg aacagttccg aatttgccta cttatcgaca aattattctc caaggccatt
    35221 ttcagctttt cttacttgac tcttctcacc agccagagcc tctctgcaaa gtttccaaac
    35281 ttactttcct tttcaacgaa atcgatattt tgttgttggg attaatctac atgaggaaaa
    35341 ctgaaaagtc aagtacgaaa ttcaaattat tttttctata tttatttttc tgttttttta
    35401 tttaacattt caataacagt tcgaagatct tttccttccg caagaagttt caatttttcc
    35461 ttttcttcga cggtataatt tcggacaaaa ctggaatcag gtatcaaaac aacgcctaat
    35521 cctgctcgtg aagcagctgt tgctcctact aaagaatctt ccactgcaaa ggctgaacta
    35581 gcttctaatc ctgttcgttc caaagctgtc agataaggaa tcggtgaagg cttgttctcc
    35641 gacacttcgt ctccgaatgt aaacgtgtcc aagtaatcag ctaatttaaa gtgatccaag
    35701 atatctgttg ctcttttcgt aacagtggtc gtagcaatgc ctagtttcat atgacgttct
    35761 tttaagtctg ccaatatttc ttttgcatat ggttttaagt ggatggctcc gcttgtcagc
    35821 tgttgataaa aatattcttc acgcttcgct cggacttcct gtactttttc agggctacct
    35881 gctgctttca ctaaaatatc aaaactttgt ttccagtttt ttccgcccca agcgtcaacg
    35941 atttcttttg ggatatggat atcaaattgc tgaaatcccc aaagccagcc atcacgatac
    36001 gttttttctg tgtcaactaa caaaccatct aaatcaaata ttgcaccttt catctataca
    36061 ttaatccttt ccaaaacaat acacatagtt tagcacgact ggtccaaaaa gaacagaagc
    36121 aaagaaccag ccttctcgtt tataagagtt cttggaatcg acggatatag attttcttca
    36181 cagctgtcac aagcgccaga taagcgatga ctgtagcaaa taaccataac cagtaattgc
    36241 caggaagtgg catcaagcct aattcagcac caaaacttgt aaaaggaagg acagaaccga
    36301 caataattcc taatgtagtg ataacaaaca tggcaaatgt tgcgttgctt tggataaatg
    36361 gtactttcgg tgtacgcagt gcatggagca ctaaggtttg tgtccatagt gattcgacaa
    36421 accatcctgc atggaataat gcgatgaaaa tcactcgctg tgaactatcc aatgtatgga
    36481 aacttccgcc cactacagca ggacaaataa caaaatacat caacaagtaa gtagtaatat
    36541 caaatatcga acttgttgga ccaaagtaaa ccatgaattc cccaatacta gatgcattcc
    36601 atttttttgg ctcgtccaaa tattctttat ccatccgatc ccatggcaag gaaatgcagg
    36661 aaacatcata gatcagattc aaaaacaaaa gttgtaaagg cagcattggt aaaaatggca
    36721 ggaacgtact tgcaatcagc acggaaaaca tattgccaaa attagaactt gctgtagcct
    36781 tgatatattt catgatattc ccaaaagttt ctcgtccaga aagaaggcct ctttccaaaa
    36841 tcgtcaaatc tttttctaaa aggatcacgt ctgcagattc tttcgcaata tccacagctg
    36901 tatctacaga aataccgata tcagccactt tcattgcagg agcgtcattg ataccatctc
    36961 ctaaaaatcc gacagtatga ccatttttac gtaaagctgt cgtcaaacgt gctttttgct
    37021 gaggattcaa ttttacgaat acttcatggt cttcagctac ttgtgctaat tcatgatcac
    37081 tcatttccga gattttctca ccggtaacca attcattgat ctctagacct acttgacggc
    37141 acactgaacg tgtaactaat tcgttgtccc cagtcagaac ttttacagct acaccatgtt
    37201 ctttcagagc tttcaacgct tgtttcgttg tttcttttgg tggatcaagg aaagcaagat
    37261 aaccaatcaa aaccatgtct gactcatctt tgactgaaaa ttctccaacg acgcttggat
    37321 tggtcttttg tgcgacacca atcactcgta agccatcttc attaagtgac tgtacttttg
    37381 caagtacggc ttcttttcgt tcttttgtta atggtgtaac agtaccgcca agatcaataa
    37441 atttagaaat gttcagcatt tcttcaatcg cgccttttgt gatcatttgt gttttaccag
    37501 ctggatcagc aaccacaaca ctcatacgac gacgttcaaa atcaaaagga atttcatcca
    37561 ctttttgata attgatctta tctgtatcta aagtttctgt tgcttcatca atgatagcca
    37621 catctaacaa atttttcagc cctgtttgat agtagctatt tagatacgca tggcgcaaaa
    37681 cacgatcatc ttcatttcct tcacaatcca tatgatattc aagaatgatt ttatcttgtg
    37741 tcaatgtccc cgttttatcc gtacataaca catcgattgc accaaagttc tggatggcat
    37801 tcaaattttt gatcactgtt ccttttttgg ccatcacact tgctcctttg accaaattcg
    37861 tcgtaacgat cattggaagc atttcaggtg ttagaccaac tgctacagat agtccaaaaa
    37921 gaaaagcttc tagccaatcc ccttttgtaa gcccattgat gacgataact gctggagcca
    37981 tcaatgacat aaatttgatc agtagcatgg atgttttatg gatgcctaat tcaaagcttg
    38041 attgaattgg tttttcagat agcgtttctg ctacatgacc aaatagtgtt tcatttccaa
    38101 cagaaacaat gattccttcc gcacttccac tgatgacatt acttcccata aagacaagat
    38161 tttcgtaact tgtttcgctt tgctgatcgt cttcacatac tttcgcatgc ttttctactg
    38221 gatagctttc ccctgtcaat gctgcttggg aaatgaataa atcctttgcg gacaataaac
    38281 gcatatccgc tggtatcata tcccctgctg aaagccgaac aagatctcca cagacgattt
    38341 cttccatagg aatctctgtg tactttccat tacgttttac agctgccgtt actttcacaa
    38401 gtgatttcag tttttcagcc gcttgattgg aacgtacgga ctgaatcaac gtcattgtcc
    38461 cgctgacgat aaccatagtg aagataatgg ctacaccgaa aagatcttta tctccaggag
    38521 ctgctaatac atattccgtg atgaatgaaa taattcctaa tgcgatcaac actaatgtaa
    38581 atggcgtgat gtatgctttc aatatttcta caatgaatgg tgtttttttg ccatatgaaa
    38641 tcgcattttc accgtaattt tctcttgaac gttcgatttg ttcttctgtc aatccatttg
    38701 ctgatgcatg gaatagttca aataattctt gtttcgtttt tccagcaaaa gctgcataga
    38761 ttttttgtgt atctttcttt ttttccatct caatctctcc tttgtgtcca aaagacagct
    38821 gttctcgctg cttttggtct ttagctattc atcggatcga tagagaaaaa actgaaaggg
    38881 gctgagaaca gatagataat agccctggcc tttcctctag atccttgttt ttcgaatgac
    38941 tgtcatccat ttgttctcat ctcctttact aagtaatttt aattcatgac ttccaatcat
    39001 ttttcgaata gttatttttc caattagacc acctcctttt tatttttcac cgatggggat
    39061 ttgcggcaac aaaaaaacct ctcgccaaat actcagcgaa aggtttgaaa aagcatatta
    39121 aaaaaagctt ctcagatctc atcgttgagt tttgacactg tacaacgtaa aaagcagata
    39181 cctgctttta gctaccttat gaaaagcctt atctcgacaa ttcctgttct acccattggc
    39241 gtctctcgac gtttttgggc agtagcctat gtttgtatag gagcctcacc taacaaatct
    39301 tattcggttt tccttcgtta atataacaac taaaaaagag gatgtcaaat caattttcca
    39361 tgtttttcat acttttttta aaatacacac gttatattcc tgtctcacgc ggttttattt
    39421 gtctcatttt ccacctcccg ctataataga aatatagtag gaaacgaggt gctgttatgg
    39481 aaagaacact tgttattatc aagccagacg gcgtgagacg ccatttagtc ggaagcatca
    39541 tccaacgttt tgaagcaaag ggactggcca ttgctgaaat gaagtttgaa actatgactc
    39601 ctgaattagc aaaagaacat tatcagcact tgactgagcg cagttttttt gatgaactga
    39661 tcgattacat gacttccggt cctgtcgttt atcttgtgct ggtcggcgag gaagtgatcg
    39721 atatcgtccg taagatggtt ggagcaacaa aagctgccga tgctgttcca ggtacgatcc
    39781 gaggagacta cgcgctgccg gggactgaaa acatcattca cgcttctgat tcccgtgatg
    39841 ctgcggtaaa agagatcgcc cgctttttcc cgccattcga tataaacaaa ccgtctgaaa
    39901 aagcggcgaa gtgatacaaa ttctaaactg ctggtctagt tgctgggttc agcaatggcc
    39961 agcagttttt tctctaagga cctccgttga ttctttccta acatatattc gagcactgtg
    40021 aagaattctt ggtagtcttt cgtcttttct tcccctaaag cagcagaaat caatctttga
    40081 taataatctg ccatcaatgt ttcatagatc actgtacttc tttctttatg tgctaagctc
    40141 cgtttcatat cttcattcac actacgtgcc tttttcaacc gaccattttc tatataaaac
    40201 gaagtcaggt tcattaaaaa agtaaagaga tactgctcat gattgttcaa ctgtgctaat
    40261 cgtcgcattc tttttttgat tttcaaataa ttgttctcaa tgaaccgatc actgaatatc
    40321 caaggaaaat cagctagaat agatagttca aattgaccat agtctggtat ctgcatcaag
    40381 tattcttcaa tgattcggca ttcataacgt aaaaattcaa attctgtctc agatacttct
    40441 gttttttcga ccatgtgaag attcaaaacc aaataataca gatcttctgt tttcttaaaa
    40501 tgcctacggt tattgctgat ctctttataa ggatcaaagc tgtcttcttg tacccgatct
    40561 agcattcgtc tttttttctt taactttatt ggttcatcca gctggtttcg catccattca
    40621 tattctgcaa gtgtcatgtt cattcgctct aaaaatgatc tcaatgttac aaatgacatt
    40681 tttttcactg aatttgtctc aatcccgact agagaagtct taaaacagat tccttccgct
    40741 aattgggact gtgtcaactt cttgttgact cgaagtttct tgatcaattc accatcatac
    40801 ataatagttc tcctttttaa tttaaatttt tccacattca ataaatagat acgttaaaaa
    40861 ggaaaaatac acgaaaaaaa taaaaaaatg tttttttcat aaaaaatacc aaccttcaag
    40921 cattagaatg ttgtctaact actgaaggtt ggtttgattg tttattgatt atttttttgc
    40981 tgttcaacgt ctgttcttgt tactggatat aagttggttg ttgttttatt accatttttt
    41041 gaaaagacac ttgtcgaatg ttcgcctttt tgcttttcga ttgcttcatt acgttccgct
    41101 tcatttttat agtcatattt tttcggatca atcgtctcaa atccgtttgg cacataaaac
    41161 cgcaagagat tttcctgatt cagcttatct gaaagactta acgcagtctt gactttttcc
    41221 tgatctttgg agatttcctc atcgattcct gctgctttcg tatcgatgat ttctcctgtc
    41281 tgattattgt aagcttttcc tcctaggacg gtgtatttgg gtgtcacaaa gttcccattt
    41341 cgaaaagcaa ctacttgatc atgctgagaa gataataagt ctgtaccaaa atgaagataa
    41401 tctttatcat cgattcccaa tagatgtagt aatgtcggca aaacatcgat ttctccaccg
    41461 tattcatgat tgactgtccc tttagtataa ccaggcatat ggatcatcaa tggcactcgt
    41521 tgcatctgtg cgttgtcaaa ttcatcccat gtatctggat ctttaccaag agcgcttgct
    41581 aaatctttat tatctgtatt cgagatccca aaatgatccc cgtaaatcac aaagattgaa
    41641 ttttggtaaa taccagattt tttcaaatac gtaaaaaatt gttccaatga ttgatccaga
    41701 taatgtgccg ttcgaacata attatctact gtgctattgc cagtattcaa agatggaaag
    41761 tcgaaattct tgtcatccgt ataataaggc gtgtggttgg ttacggaaag aaatttcgtg
    41821 taaaacggct gttgtagatg ctcaagatat tgggcagatt ctctgaataa atctttatct
    41881 aatatcccat aacctaacgt ttcgtcggat tcatcaaaat aagatcgatc aaagaaattg
    41941 tcataaccta agtttttata tacatgatct ctattccaaa aactagctac attcccatgg
    42001 aaaacagcgc ttgtatagcc ttgcttttgt ccaaggatcg ctggtgcggc ttgaaagaca
    42061 ttgtctgacc caagttctgt gaataatgag ccttgaggta aaccaaatgt gccagtttcc
    42121 aacatatttt cagcatcact tgtctttccc tgtcctactt catgaaagaa attgtcaaag
    42181 ctgatcgttg cttgattctg gaaaatacta tttaaaaacg gtgtgacttc ttgtccgtct
    42241 accttcatat taatcaaaaa ctgctggaaa ctttctaaat ggagaacgat cacatttttc
    42301 cctttagcga tcccaaatgt ttcgggattt ggctctgcat aatgtttctt tgtataatct
    42361 aaaacttcat caatcccatt actgcttgca tgcgcgcgaa cactgctagt cattcctgtt
    42421 ttgattccgt cataaatagt atacgcatcg attcctagat atttgacaat atatgaacgg
    42481 tcaaatgttc gctgcagcaa ttgtggacgg ttggcttcgc tcagcgctag atttcctgaa
    42541 aaaataagcc ctgaaagaca ggtaacagcg atagccatcg gtttgccgac agggttgctt
    42601 ttgataggta ttttcttgac cttcaaccaa accagtagcc caatgaacac agcgatatcc
    42661 agccagtaga tgacatcatg gggcttcatc aaagaaaagg agcttcctga caatccttgc
    42721 gacactttcg aaaagcctaa tacagatttg actgtaatga aatctgtgaa ttcccgataa
    42781 aaaataatat tcaagtacaa caaaagcgta tttaaaatat caatgatcaa aagaatagct
    42841 aacgctggct ttggcttttt aatatacaaa gaaaggccaa aaaacaataa ggtagtcgca
    42901 attgggttga tccacaagat aaagtactgg atcgtacctt ccacacctaa tgaaaaatct
    42961 gcatagtagg caatgatcgt cttcagccag aaaaagaaaa cagctgtcaa aacgattgct
    43021 aaacgatttt ggcacaattt tttaagttgt gtcacgtata tcacttctct ctattcacat
    43081 tttttcttat tttgctcctg aaaaatgaaa atttcctaaa aaagttgtta tcttaagatt
    43141 tttttattct ttctaaagat tattccgtcc acttttcaca gcataaaacc gctgcgatgt
    43201 aagcgattaa actaatgaag agataaaagt cttgtggtga ccgcttcact ccacggtatt
    43261 caaaagccag tttctcggta ttcgtacaaa aaaagaggct gtgacaaaat tcttgtcgca
    43321 gctccttatt tcttgatgca ggacagcttt ttcacaactt cttccttaaa cggtgttctc
    43381 ctgccaactc ctcggccaca agtcacaata tttataaaac atgagaagct gttttttgaa
    43441 attgttcctt gtggctcaga gttaaacggc aggctcaaga cctcttgata cacggtgttc
    43501 aatcagctga ccctcggtat tagattatgt caggacgcta attttacaac ctcttggtaa
    43561 acggtgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa atataaggag
    43621 ctattttttg aaattgctcc ttgtggctca gagttaaacg gcaggctcaa gacctcttga
    43681 tacacggtgt tcaatcagct gaccttcggt attagctcaa aaaacggcaa aatatgagga
    43741 actattttgc cgtttgtttc tctaatactc aggtcaaaac gctgatttca caacctctgt
    43801 ttttcattgt ccataggatt gatattgtcc ttgtgatgac tcattcgtaa taattgaggc
    43861 ttgattttct tcacttcccg ttatcgtttg tccactgcca gaatctgaag gacttgttcc
    43921 tgtcgactcg tttttttgag aagagacagt tgttttcttg acttgttcat ttgtcttttt
    43981 ccattccgct aaacgatcgg cgaccacttt tttgtctact ttttcagaat aagcggtgat
    44041 caaacgggct actttgtcgc catcatgagt caaaagcaaa acggtcaaat gcttgtcttt
    44101 aaattgatat tgtctgagag aaatcgcact aagtacgtca tcgatattaa taaattgatt
    44161 ttcttctttt ccttttggca aataaatgtc caattcgcct ttttcgtaat tggcagttat
    44221 tttatactcc gcccaccaat aagttgagaa ttcttttttg aaattatcta ctgattcttt
    44281 gcttgctacg attttttctg tttctttctg agcaagatct gctgtcattt ttcctgaaaa
    44341 tgctgcggat aaaataagca gtaagaaaaa accgaataac caaaagagaa gaaacggtct
    44401 ttttttcctc atgctttcct ccactcctat ttaattttga ctattctatt atatacaaaa
    44461 aatgaaatag aaagacaaaa aagttgaaaa agatcgtaaa taattgaact tctttttcac
    44521 aaaatggtaa gaatggagaa tttcacgcat ttgctctcaa ctattctttc ttaagcctca
    44581 aatccgtttt tatctgatac gtctttgaac caatgacccg attttttgat tgttcttttt
    44641 ccatcttgtg ctaagtctac tgaaataaag ccatatcgat tcttatatgc attggtccac
    44701 gaccagttat ccatacatgt ccacatatga tagcctaaac aattggcacc ttcttgaatg
    44761 gcttgatgga catatttcag atgagattgg acaaattcga tccgataatc atcgtggatc
    44821 atgccatctt ctttgataaa tcgtgcttcg ttttctacac ccataccgtt ttcagaaata
    44881 aagcagtcga tatttccata atgatctcgt acattgatca aaatatcata gattccttgt
    44941 tcatagatct cccatccgcg ataaggattc attttacgac caggcatttc ataattgtcg
    45001 aaataatcct caggcattgg accgttttta gtatcgattg gtgtttcttt tgcttttacc
    45061 cggcgtggct ggtaataatt gatacctaaa agatctactg tattttcttt gataaccgtc
    45121 aaatcctctg catggatcac aggcatgaga tcaagttctt ttactatctc aatcaattca
    45181 cttggaaact cgccttttac agctggatca aggaatgaac gattgaagaa cgcatctgct
    45241 atttgcgctg ctttaacatc ttcagggttt gattcgtctc ttggatagct tggtgtcaga
    45301 ttcaagatga taccaataac accaccttgc tccatttcat ggtaagattt gatggctaac
    45361 gctgaagcca acgcttcatg atagccaact tggatcgctt ttttcatatc tgactcttct
    45421 ggataatgcc atcgatataa ataacctgct tcgactggaa cgattggttc attatgagta
    45481 aaccattttt ttactttcga accaaacaat tcaaaacaac ttttggcata tttttcatag
    45541 gcttcaaccg tttctcggtt caaccatccg cctttttctt gcagatacat cggcatatcg
    45601 aaatgataca gattcacaaa gggttcgata ccgttttcaa tcaactcatc aaaataacga
    45661 tgatagaaac gaacggcttc ttcgttgatt gttccagatt catcaggtat caaacggctc
    45721 cattggatag atgttcgaaa tgaattatgc ccagtctgtt tcatcaactg tacatcctct
    45781 tgatacttcg tataaacttg cgaggttttt tcaggtccta cttgatcaaa gaacttttgc
    45841 ggttcttttt tgtaccaata atcaaaaatg ttctctcctt ttccatcgcc tgaaatcctt
    45901 ccttctgttt gtgggccact tgctgcagaa ccccaccaaa aatttgctgg aaattgatat
    45961 tttttcatca aagcacctct ttttcttttt tagttcgttt tagttgattc catgtatgat
    46021 attttccatc acaatcactt tttcattttc ttcctcttct tttttgattc gttttaacag
    46081 ctgttctgct gctcgttccc cgattttatc tgttggtggc gtgatcgtga ttttcggaag
    46141 gatactcaga tcgtttttat ctaaataatc atagtgactg aacgcaatat ccttcccgat
    46201 tctgacacct tcttgattga aacattcgat ggcaccaatc gacatattgt aattgctgac
    46261 aaaaatagca gttgcccctt tttctaataa tgcttttgcc ccgtaatagc cgctttttct
    46321 agaatagtct cctgtaaaga cctctaattg gatcgatgga tcattttgga ccgcttcttt
    46381 ggctcctgct aaacgttctc ttgctacgta gtctgtttga gtggcagcaa taaatccgat
    46441 ttttttatgt ccttgttcac catgatgagc gatcactttt gctacacttt ctcgatcatt
    46501 cactaatatt gtatcaatac ctgctggtcc ttctggacaa ttcaatgcga taactggaat
    46561 actcattttt gtgagcattt caaatccaga ccactcttct tcggataaga agacaatcag
    46621 cccatcgatc acatgcttca tcaggtattc aattttttgt tcaatttttt ctggctgatc
    46681 ggcaaacccg cttaataata agctataccc attttcttca gctactcttt cgatccctga
    46741 gacaacttcc gacccaaaac ggctggaaat cccgtttatc aaaagtccaa tagagaagct
    46801 tcgattattc ttcaaccctt tagcaatgat gttttcttta tagtccaacg ctgaaatcgc
    46861 ttctgctatt tttttcatgt tcttttcctt cagttgctga ccattcaagt aacgggaaac
    46921 tgttcccact gaaacaccag ccatcttagc cacatcacgg atcgatgcca tacattctca
    46981 ctcctgccag ttacatttga tttctctttc ttgttgttcg attatgatga ccgcattctt
    47041 tgcatagtgt tgcttgtcat caatatccat ataactaaag tcttcagtag agcaacttcc
    47101 tctcactatt aattatactt aaacaagtat ttgaaccggt tcatttttaa tatagcattt
    47161 ttttaaaaga ttgcaacact tttttaaaac gctttctgaa agtggcggat tttttgcctt
    47221 tttaaaattt tattctaaaa tttactctat ttgtgggtgt tttgtgcgtt tttctcttcc
    47281 atccgtttga agttttacat tccaatggta taatttcttt atcctatcaa atgggggaag
    47341 tattttgaag aaattaatcg tactatttat tttgtctgcg ggacttttga cattgactgg
    47401 ctgtgatttg tttggcggct ctgaccaatc ctcggacgaa acaaccgatt cttctgtctt
    47461 aaaagcggaa aaagcagata ccaccaacag tgtatatacg ctaaaagtac gtaaagatat
    47521 ggatttatcc gcgggacagt tgacatttac agataaaaaa atggaatgga cacgcacgta
    47581 tcagacacaa ggaactgatt cttctgaatc atctgaagaa aaaacagtgc tgacagatat
    47641 caagatcact accaaagatg acgaatatat tatttcagga aaagaaaata aaaaagaaac
    47701 aacagtcata tttacaaaaa tcggtaatta ccgtttgaag gatgaggatg gcaacatcta
    47761 cagtctatag tgccgtctgt tggatcttct tttgtttctc cccttctttt ctgctatagt
    47821 aaagaagcaa gatgattgta aaggagacga aatggatgat cgatagtcct gattattgta
    47881 catgtaataa caacaaaaat atccatacag aatttaatga ccaaggaagc ttgagcatat
    47941 gcgacaactg tcacaaacta gtcaaagatt catttaaaac atttgaatct tatcctgttc
    48001 aaaattatgt aaaaagtaga agaagataaa caaatccaag caaaactcac tatggctgat
    48061 ttttatgaac ttcaaactca gcttcttcct ttgatcaagc ttcatcagta aaacaaacag
    48121 tggatcattt cagtttacaa atcaaagagt aaagatttat ttatttcaaa tatttgaata
    48181 taaaataacc aaacgataga aaggcttatt ctacatcacg tagtaatttt tctttcccgt
    48241 cgtttggttt tttattttta gcctaattct tgaccttttg catgctggtt atccgtaaaa
    48301 tattttccag caggctttcc ttccaattca gctaattttt cttggatctg acggatcacc
    48361 ttttttgcat cttctggatt ttctacaaaa tcgaatttat ccccatccac ttgtatttta
    48421 ggacatacat caaaatcttc ataccattga ttatagcggg tgttcaacgt ttcataatag
    48481 gtatataacg tctcatcgct ttctagctgt tcatagtcac gcccacgttt tttgatacgt
    48541 tctagcatcg tgtccaaaga aactttgata tggaccatca aatcaggccg tttttttgga
    48601 gcaacatcat ctaactcgtt tagcatcgta tctaataagc tatcgtactg ctgtacttcg
    48661 atatccgtta cccgacctaa atcagcgttt agatggaaca acaggctatc ttcatagatg
    48721 gatcggtcta gcacattgtc atcgttcact aaagcatttt tcattgctaa aaatcgcttg
    48781 ttcaaaaaga agatttgcaa taaaaaagcg tactgctcag gatttgaata aaagagcggc
    48841 aatacttcgt tatcttcgac attttcataa aatggtttag aatcaatctc ttttgcaagc
    48901 atatccgtca aactgctctt acctgcacca atcgttcctg aaatcaaaat cactgacata
    48961 caacccttcc tcttctataa gtactcgcct acgtaagttt caacaggcaa tgtttcaaaa
    49021 tcatattcac gtacagcttg gtctgatttt aaatacgtat ggagactttg tgtaatcatc
    49081 aagttcgtag gtaccgtctc ttcttgtaca atgatcatcg gttttttcaa catagttgcc
    49141 acacctaatt cgtaagccgt tcctggatcg attgtttgat gctcaaagtc aaggatagca
    49201 acaacaaatt ctgcatttgt cagttcttcc atatcttttt cgtatacttc ctgcgcccat
    49261 ccagcgctga atagttcata attgctttct tggtgatgac gtggagagta gaaacttgtg
    49321 actgttttgt tttcctccaa cgctttttcg atacgactta ctcgatcgat ttgctcctct
    49381 gaaaaaaatg gtcctgctaa atatatttga ctcattattt tgcctccttc aataataact
    49441 gatttttaat agatactgct cgtaattcat tgaccagttc caatggattg ttggaaaaga
    49501 ttacttgatc caaccatttt ttatccataa aatcctcttt tgcaaaagtt tctaataatt
    49561 gttgcaagcc gctgtaataa ccgccaatat tcaatattcc gattggcttg tcgggcgtca
    49621 gaccgattgc tatccaagaa agaagttgtg agaattcctc caatgtacct agacctccag
    49681 ggaaaacgag gacagcatct cccttttcaa ataataatac ctttctttca tcgatcgttt
    49741 ctgtaggaat aaatgtagta acatcttttc caggcaattc agtttcaaaa agcccattcg
    49801 gatagatacc tgtgactttt ccgttatttt ccagtactgt tttactaatc actcccataa
    49861 tcccggagta tgaaccaccg taaaccagtt cgatttcttc ttgtgccatg tattttccta
    49921 acgtttgcgc aataaatttg taactttctt tattaccaaa acgggaaccg caaaaaacag
    49981 taagtttcat aaaaaaacac tccctaactc aaaagtctat cttcattcta cacgtgtttt
    50041 tttaaaataa aagactagat tttgtagttt tttagtggta tataggatag acgccactat
    50101 atatagtatt tagaaaaaaa tcatttttca gccattttat agataaaaca aaaataaata
    50161 acgtgttttc atcgatatga aatgacttac ttatagaaga tactgggatt tcgtcctata
    50221 tgccaataac gtgtattatt ttaaacaaca aatacttgtt tcttttatta acgaaaaaat
    50281 cattttgttc tcgtgttatg tttcactgat aaatgatgtt tctctaaata aaaaagccga
    50341 cgaactaaac gatcaaaatt tgatcatttt tgttcatcag cttctcctaa aaatcttttt
    50401 gttaacgata aatagctgga tttgtaaaaa tatcacttga atcgtcactg ctggaagaaa
    50461 attcagtgat aaccgcacct gttgcgcctg cctggaacta gtgtttgata ttgggctcga
    50521 acgtgtactg atctctagca tgaagaataa tttctttttt tactgtataa taactttctt
    50581 tcctttttgg aatagtacag gaaagttttt ttgtttcctt tccttcgaca taaggtatac
    50641 cagacccttt cgacaacgaa aggttctcat ttttccttca tcgtcatttc aaggagggtg
    50701 ccgatgctct ggacagtttc gatcgctcct aaaccaaaat ctgtatattc gattctttct
    50761 aactctgatt ctttcatacc attcctgtct caaggtacat ctattgtact tttttcttgt
    50821 aatccattct gcctctccta actaattgtc aaatcatccc aagatatgtc ttcttcctct
    50881 ttttcttctt ggatgatttc tgcattcgga tctactggtt ttttcaataa taaaatggtt
    50941 attcctgtga caaaagaacc agctaacaat gcaccaatga atgcccatgg tctggtcatc
    51001 gtagcgactg caaacattcc cgatgccgga atacggctat ctgcgcccca aaacatgctt
    51061 aatccgccac ctactgtccc accgactccc gttgctatga cattgcgtgc caagtcgttc
    51121 aatacgatag ggaaacatcc ctctgtgatc tgacagatcc ccataggaaa agcggtcttc
    51181 aatgtatcga tttcttgacg attgagtata tttttacgaa tgattttccc taagaaataa
    51241 gcgatcgtca agccgaatgg ggttaccatc gaagctaaaa tcaatacagt gattggttca
    51301 tagattcctt ctgaaaatcg tgccacaccc ccttatccag aataaatggt aatccagaaa
    51361 cagccgtgta tctgcggtaa taagcctaac caaacgaaaa atatgagaag ctatttttgt
    51421 ttggctgttc ttattacttg atgcagggca gctttttcac aacctctttt tttatttatg
    51481 tttcataatc aatatggtaa ggatgcagct ccgtatggta gaccgcctcc gaatattcaa
    51541 tcatttcccc aatttgcgta gacgatttcc gtatccgttt gatgccgatc tgtgcgtctg
    51601 ttttcagaat tccttgttct tcttctgtta aaaatactgc cgagaaatca tcagaaaatg
    51661 agtcaatagt atatcctttt ttcgtcagca aacgatacaa agattctttg ccaaattctt
    51721 ctctgctgaa ttgcatcatt tcttgtggaa gataatgaac aaaataaata taaggctgat
    51781 tgttcaaata atataagcgc ttaaaacaaa cagcttgctc accgaaaaag gaaaacaaag
    51841 gatcttcctt ctttatatgg accaattgca aatccagcgt ttctttccgg acttcatatc
    51901 gggagttctc caagatttgc gtaaagcttg ccgctttgga taatcgattg taaggacgat
    51961 tgcttagaac cgtcgtccca cgaccacttt tctttgcgac atattcatct gatgccagca
    52021 gttcgattgc ctttcggaca gtgattttgc tgacttgaaa cagttcttct aattcacttt
    52081 ctgtcggcaa catcgaaccg actggatatt ttcctgagaa aatatcttct ttaattttat
    52141 ctgcgacttc caagtatagt acacgtgtcc ttttcataac gtcacccttt gctttactat
    52201 tactaaaaga atagcacata aaaaataaaa aaagaacaca aatcacatgt gagaagtgtg
    52261 ttctttttca ttcagctgct ttaattacaa gtttacgcca tttgaggcaa ttacttcttt
    52321 ataccaatca aaagattttt tctttgatcg attcagtgtc ccatttcctt catcgtctag
    52381 atccacatag ataaagccgt aacgtttact catttctcct gttgatgcac tgacaagatc
    52441 gatacatccc caaggcgtat agcccatcaa atctacaccg tctgctactg cattagccat
    52501 cgcttcgata tgacgacgta aataatcgat ccgataatca tcaataatcg tgccatcctc
    52561 ttccatatga tcaatggctc caagaccgtt ttcaacaatg aataatggtt tctggtaacg
    52621 attgtataat tcattcaatg cgattctcag cccttctgga tcgatctgcc agccccattc
    52681 acttgcctca agaaacggat tttttactcc tcccagcaag ttcccttctg aagttgctgc
    52741 ttgtggatca gtctcattga ttgccgtaga catataataa ctaaatccga tgtagtccac
    52801 tggatattct tttagcaact ggaagtcttt ttccgtttgc tctaattttt ccggatcgac
    52861 tccatatttt ttatgggtac gcgctgtgta tgcagggtat tctccacgta cttggacatc
    52921 cgtgcagaaa aaattgaatt cttggttttg gatcattgtt gctgcctgat tgactggatt
    52981 ggcatcgatg ctgtaagttg ttgcatagat aatcatgcag ccgatttgga tatccgaacg
    53041 caattcgtgg ccgattttta cggctttgct acttgcaacg aattgattat gccatgcttg
    53101 gaaaatattt tggtactctc ctgctccatt gctttttact aatccttgac ttaaagctgg
    53161 aaagtgaaag gccgaattga tttcattgaa agtcatccaa tatttgactt tactgctgta
    53221 tctctctaat actgtctgag caaatcgttc atagaagtcg atcagttttc gatttttcca
    53281 gccaccgtat tcttttgcca aatgaagagg catttcgtaa tgagaaatcg tgatcacagg
    53341 ttcaatatca tattttaagc actcatcaat cagctgatca taaaattcta atcccgcttc
    53401 gttaggagta ccttcgtctc catttggaaa aattcgtgtc caggcaatcg aaaaacgata
    53461 acatttgaat cccatttccg caaacaaggc aagatcttct ttgaaacgat cgtaatgatc
    53521 gattcctcga tgattcgggt aaatatattt ttcttgatca atcgtccagt caaattcttc
    53581 actgccgata atcgcaaagc gctgcttacc tccaggcata gcatctgcaa cggatagtcc
    53641 ttttccgtct cgatcataag ccccttctaa ttgatttggc agcagttgct ccaccccata
    53701 aaaatttttc tgaaaaacgt gattgcatcc tgatttcctc cttattttac aattgccaga
    53761 acatcttcgt ttgcaataat ctctttttgg tctaattcaa gtatatcggc aaatgcatca
    53821 gtattcgtaa tgacaactgg agtgattacg ctatagccag ctgctctaat tgcttcgatg
    53881 tcaaaacgaa tcaataaatc tccttctttg acgagatccc cttgtttgac taagatctca
    53941 aagccattcc catttaattc tactgtatcc atcccgatat gaatcaaaat ctccgctccg
    54001 tcagtcgttg tgatccctac agcatgtcca gttggaaaaa tagtcgtgat ctctccgtct
    54061 gcaggtgcaa ctagttctcc tacttctgga tcaatagcga taccttttcc catcacgcca
    54121 gaagaaaaaa cttggtctgg tacttcactc aatggcagta ctttacccgt taaaggactt
    54181 tttaaggtat gccgtgctga agtaatcggt tctcctgcat tcgcatgttt gttttctaat
    54241 tgattgtctt gtgtttgttc gtcaaatcct aaaatcaatg tacctacgaa tgcaagaaca
    54301 aaagcaattc ctgtagctat tactgctact gtcacatttg actctacacc atccaccgta
    54361 ttaatgaaag tcggaatcgt caataagctg accaaactgc tggagaaagc ttttactccg
    54421 gaaaatccga taatagctcc accaatacct ccagaaatac aagcggcaat gaatggtttc
    54481 ttcaaaggta atgtcacacc atacacagtt ggttcagtga ttccaaataa cgaagtcact
    54541 gtagaagaaa tccctaatgc tcgaagtttc gtatcttttg ttcgcaatgc tacagctaaa
    54601 gcagccccac cttgtgccaa gatggctgga agaagcattg gcatcaatac atcaaaccca
    54661 tattgttcga tattcaaaaa catgatcggt acgaatcccc gatgcatgcc gaacatcacg
    54721 aatacttgcc ataaacttcc catgattaga cctgctacga ttggactaaa tccataaatc
    54781 gagttgaaga gaccgcctaa agcattaccg gcaaccgtcc cgattggtcc taaagcaaga
    54841 aatgttaatg gaaccatcac tactaaaacg acaagcggaa ctaaaatcat ttgcagaaat
    54901 tgcggaatca ctttttttac gaatggctct aatttacttt gaacccatac ggctagaatg
    54961 attggtatga cagaagaagt ataaccagat ggtgaaagaa tcactgggat cccaaagaaa
    55021 ctgatagctt ctcccgcacc agcaactgct gtgatagctg gatacacaag tgccatagca
    55081 attgctactg ctaaaaactg gttggctttg aactttttag ctgctgtaaa agcaagcatg
    55141 acaggtaaga aggtaaagac accatctgct tcagcaaaca atatttgata agtaccgcta
    55201 tcagctgtca gccagcccat caccgttgcc aatgataaga atcctttcaa taccccggcg
    55261 gctgccatcg cccctagaaa aggtgtaaat atactagaga taatatcgat cagacgattc
    55321 aaaatatttt tggactcttc ttttccatcc tccgaaactg caagaccttc ctcatccacg
    55381 atcgattgat aaacatctgc tacattactg ccaatcacta cttgatactg accgccactt
    55441 tgtacgactt ggatcacgtc cgggtcttct tttaactttt gcgtatctgc taaagattca
    55501 tcttttaatc tgaatcgcaa ccgcgtagca caatgtacta aacttttgac attcttttgt
    55561 cctcctaccg cttcccatac acgacgacca actgcttgat tatccattgt tttttccttc
    55621 tttctttttt atttaaaaaa taaaaaccca gattgaaaaa aacagtcaca aacattcgtg
    55681 aacctatttt ttcaatccag gttttgcctg ccatacagta acaatcctta tgattggatg
    55741 ctttttttct cacttactac tctttgaata tgaatcgtta aataaatctg ctctgcgatc
    55801 cccatttcct gtttttgcgt ttgtcgaaga tactcattga tccgttcgac acaatgaaac
    55861 gcttttggat actttccttg aactaatgcg tagagaaatt catcttgctc gtcagatcct
    55921 tcccctttga gataacgctg ggcaaaaaac tgcagatgag taatgatacg ctgataattc
    55981 aaagattctt catcgaacac gatgccgaag aatttactga tgatactcag tatatctctt
    56041 accattgtgg tagcggtcat cgtcacttgc atgtcttcgt ttccttgttg gctattcaca
    56101 aaatgaaagg caataaaacc ggcttcatcc tcagaaagtt ccacacccgt cttttgtttc
    56161 acgaacaaaa gagcctcttt cgctgcttga tattctttcg gataaaattt ttttgtttca
    56221 aataataatg gattaggcag ttggatattt tcttttgctc gtgtgatcgc ataatgaaga
    56281 tgatcggtca atgtcagata aatattaggc gaaaattcaa cttttagttt atcttcagcc
    56341 agttcgacta cttgtttgac ccattcgatc tcctctgccg gaatatcagc catcagctgc
    56401 tggaattggt ttttagcaaa agaatctctt aaaacaaatt ctttttggac cgcttctggc
    56461 gaaatctcgt ccccaatctt tttttgaaaa gctagtcctt ttcccataca aacgacttct
    56521 tccccgaatc catttttcga aatgacgaca ttgttattca aaattttatc aattttcatt
    56581 ttgcttcgcc ctctcaagaa aaataaaaaa acctagaatt cgcctactgt ttcttagctg
    56641 aattctggtt ttgcctgccg tacagtaaca atccatttct tattcaaaat catcatacca
    56701 tcgaattttt atttatgcaa gccttttcat ataaaaaata aaaaaactgg aacacaagtc
    56761 tatctagaaa acaaccaaat cagagatgca cctctttgcg tttctctttt tttcagcaaa
    56821 ttggtttttc cttatcgatt catatccttt ggctgttttt aatctgtaac gacttatgtc
    56881 ccagtatcct aaaatgtttt tgtctctaga ttgaaagcag atctcatatc atctgtcgca
    56941 tcttttatcg atgtaacact ttccagttct ccttgtacaa ctagtcgcgt gacggcgtcc
    57001 atgtcgccac aagtaattag tgtgacaatt tcttttccct cgacttcatt cagtaattgg
    57061 gtatctgtag gagcaacttt ttctttaaaa aatgttttgt atgtatacac attttctaaa
    57121 tctgtcaaat agatcttggc acccatctct aaattttcta gcggtgtaaa cagcaattca
    57181 gggttcgttg cacgatggct tgctaaggca taatttcctt ttcccatttc ttgatctgga
    57241 gataatgttc ctgctccgta aagtagtgac gtattatcca agcctttgaa aaccggtaaa
    57301 ttgattgaaa ctgaagggac tgcgatacta gcaatcactg gtagatctgt tcctctcatt
    57361 tgcgacctca tgaccccttc tgtcgtcatt ggttctaccg catcaaagtc aaaactgaca
    57421 tctttatcgt tattcttttt cagatcttct tttgttacat tcgctatagc atatttatct
    57481 cctgtctctc tgacaaaata atccttgatc tgttcattga aaatcaaagc tagtcctact
    57541 aataacaaga ggaacaagag gatattgact agccatcttt ttgcttttcc tttttttttc
    57601 attttctctc cctccagact cattttactg tttttctttt gctctttcca acagccgaat
    57661 gacacatttc tacaactctg tgtatttttt tcgtacacct gcgctttttt ctacaggata
    57721 tttcaaatta tttttgatca atttttcttc aatgatctca tctagatcca ggtgaaggtt
    57781 gtctgccatc atgtatgaat agatcaatac atctgccagt tcttctttga ttcgttctaa
    57841 ttgtgtttct tttacttctt ccggcgtttt ccattgaaaa agttctaaca attcagatgc
    57901 ttccaaagaa atagagatag ccaaatcttt ttcgttatga aattgtcgcc aattacgttc
    57961 atctctaaac tggttgactt tttccatact attcatttgc ttgccttctt ccaatcactt
    58021 cttacttgat taatcatacc atttttttac ataaaaaata gatgatgctt ttaaaaaaac
    58081 tcctgttttc ttttatttaa aataaatgtc atagaaaatc aataatttag gtagccacca
    58141 tgtgtatgca gtagcgtgtc ttcttccctt gaggcttttt gtttttcgat acgttcttct
    58201 tcccaaacag acaacgctac tagaatctct cttaatgatt ttccttcttc ggtgagcgag
    58261 tactctactt taggcgggac ttggttatag gcttttcgat ggatactttc atcttgttct
    58321 aactctctta actgctgggt cagcattttt ttgtgatcgt agggatttgc ttgcttctcc
    58381 acgcctaaaa taagcgatat tgctcttccc tttatctggt gtaagcaaag cagttagcat
    58441 tttgacgatc gtgggttccg cttttcactt cttatctact tagtatcatg aagtatactt
    58501 tcgtactaat actaagtagc ggaagtgtca actactttgt ttaactacat agcaaaagaa
    58561 acagaagggt gacaacggtc atcacgcttc tgtttctttt gaatatgctg tgttgttgtc
    58621 tctatctccg tgtagacttt cctcaaactt atcctttgac agtaagtcag taggctgatt
    58681 tgctctttct agatgccaga tacatttcta caaaaaaata gtgattctgt tttgaaaatt
    58741 cgatttttag ttctgcattt tcgtaaacat ccaacatttt tttcgcactg tatagaccta
    58801 acccgccatt ttttttagag gtttttcctc ttttgaccat ctccgatagc gtcaaattcg
    58861 ttggatttgc cttgttcgct atactaaaac gtgatccctc tttttcttcc agcagattga
    58921 tcgtaatcgg actctgatcg gctacacgat gatcgaatgc gttagaaata aaaatagata
    58981 aacagcgaat aaaaacaacc agatcaatcg ggattgtttt cacttcgaca ggaataacta
    59041 cttggatcgg gattttctcc gattctgctt tttctagaaa ttttaaaaag acacccctga
    59101 cagctgggga tacaatattt gttagctgtt gtatttgcgg tttttcaaaa atagctgcag
    59161 aatgatcttc taatatttct aaaaattcca acgtttcttt tttattttcc tgaagagtaa
    59221 tttttaatga aaacagtaga cttttataat catgtttgaa ttcctctaga tgatggtaat
    59281 attcttcatt tatttgatac agttgaagcg attcctgatg ttgtctttct tttacttgga
    59341 aactgatcaa cataatggaa aaaaacaaag aaaaaaaact gatgactatc gacaaaagag
    59401 atagaagcag ataattagat gagcggaagt gaacacttcc caataccaag taaatcacgc
    59461 cccagcttaa taaaatacaa atacctccaa aatagtgttc agttggataa gtaaaaagca
    59521 agttgagaaa tctcgtctgt ttgtctatac tttttactaa tagatagaaa ataaatgcga
    59581 taagtagtgc aaaaaaactt gctgttccaa gatgtctctt tcctaaataa tccatcagat
    59641 caaaaaccag aaaaaagctg agtccagtga tagcaaaaaa aagattactg atcaaacagc
    59701 tgataaacag gtttttcgta gtaaaataaa aaagaaacat caataataag ccaactaggt
    59761 aaggaaagct cactttaggg ataggaagta aaagtgtcac caccatcaaa gctatacaac
    59821 caaagctgtt ttttactagc agttgtttcg aaaagaataa atagatacct aaatctaaaa
    59881 tcaaagtaaa ggtcaaataa agtgtcactg ttgcgaccat cttattcctc cattgttttg
    59941 tttcaaaatg tacacatatt tttttaatag gagcctgcat atgaaaaagt attttatcat
    60001 agaagataac cagtttcaca gcgaatggat catcagagaa ttggtcaatc atcatatcga
    60061 gaatatcgtg cagctcactt ctatctctca aatcaatcag ttgatccagt tgggcactat
    60121 tgataatcac gatgttttct ttatcgatat cgacttgaag caatcactca caggtattga
    60181 tgtcgccgaa caaatacgta aaaaaaatgc acatagtaat attgtttttt tcaccgctta
    60241 tacgaatttt gcagtagaga tcatcaatcg gaaaatcctt ccgattggat atatcagaaa
    60301 agacaggaat ttatctgaac aaattcgtca agtgctagaa cagatcaatt ggaaagaaca
    60361 gcagatcgta aatgtcgata aaatctttat tgatcaaaaa ggtggaagtc tcgtcctgta
    60421 tcctcgggaa attttatata ttgaaagtat caaaggtttg aaaaaccaag ttatggtcaa
    60481 aacaactaca gaagataaat tagtgaatac ttctttaaaa caattgaaga cagagttaga
    60541 aaagtatgct tactttttac ttttaaaatc ttactgtatc aacttatcgc aattgcagaa
    60601 aattgattct gctcattatg tgttagaatt tttcaacggt gactccttac tcgttggtcg
    60661 aaagatcttt gaaaagacaa aggaacgttt ccatgacttc caaaaaactg cttcgagttg
    60721 atgaataaga gctgagacgt aagcatagca gatttcaggg agtgggcctt gaatcggctc
    60781 cactccctga aatcgttgat atgattttac gcttcctctt ctgtaggagc atctgttaac
    60841 gaccatgtga atgttgaaac gtagttgtct acagcataat tggatacagg aacaaataat
    60901 tccactgatt gtctcccttg ttccacattc tctcctaatg aataaatcca tgtgccaatc
    60961 ccttcatttg ctcctgcagt tactagatct actgcctgat ctgctgggat ttcgattcct
    61021 tcagcgccag ttccagaaac ggtagatggc attccactgc tattagttgc gctatttcga
    61081 atcacggcgt ttttgaattt cacctttgaa ccagttagtt tattcgtatc agatgttgta
    61141 ccaataaatt ctgttcgttt gactgataat ttccaaccag cataatttcc gcttttatcc
    61201 gttacttgta cgaaattctg tacttgtgtt tgtgctccag tactgtccgt tccgctttgt
    61261 aatgcagcat atactgtttt tgctgtacct aacttgattg ttccaaaatc aagatctgga
    61321 gcttggtcaa tcgtcagtaa tccggatgtt ccctgatttg gttcgtcagg tgtaaaagga
    61381 gtatcaggat catttggatc aacaggagga actggaacaa cgtcttcagg atttgcagca
    61441 tctggtttgg tcacagaaat cgttccttct gtagtggctg agctcttatt ctcagcaaaa
    61501 acactttgtg tacataataa tagacttcca gctagaacgg tacttaatgc aattgttttt
    61561 ttcatttctt tttccttctt tcttttaaaa taaataatcc agcaaataag ccaataagaa
    61621 tgactccaac aagtatcagc catcgccagt caaatgtttc tggctgattt tttaaatcaa
    61681 tatccttttc attcaattgg tccgctgcat catcagttat ttcaaaatcg gtatggagat
    61741 tccagcgttt ttctttccaa gcacccttca caaccgcttg ataatgtccc ggagctaatt
    61801 tttttccttc taatgggaca agtaaatcaa aatttgtatt gggtgctaca tctatttttt
    61861 ctttttttat tgaaaggtaa ggctcttttg tttctttttt gtagatttca accgatgtag
    61921 tcaagccgaa catataagaa gcatttggat tttgaagatt cgccttaatc acatttcttc
    61981 catcgctttg atccgcataa gcttttttta atttcaaatc tggggaagac tctttttcgc
    62041 tatttcaaat caagattgct ttcacttgag aaaactgatt gacgacacca actgtacttt
    62101 tgttagaaac gttctctttt ttttcttgaa acgtgatccc tcctgcgacc acaccagcca
    62161 gctctttttc tggcgctttc agtgaaagtg tctcagtgac cgcactttga ggaggaacag
    62221 ttatttctcc cactgttttt gctaagtttt tcatatcata acttaatgtt tcatcaaatt
    62281 tactttttga ttctccatat tcgatcacac cgtttgaatt cgtcgtagcg gagtggatca
    62341 atgtctcaac tgttactgat ttatctgttc ggttaaagac cgtgtagggg atttcctgtg
    62401 ttttcccagg ctccatcctt acatttatat atgttttgtt cttatcaatc tgattagccg
    62461 gtaattcaaa atctaccgca aaattgaatt cggaggcatg gacaggtgtc tgccaaagga
    62521 tgaatccaag gagaaggatc ataagtaaag agcagttgtt tcgatgactt ttattccata
    62581 ttcgattcat caatcatcct ccttgatctt tattctgtcg gcgcatctgc taatgtccat
    62641 gttatctctg ctgagtaatt accagctatc tgattttctt tggttaagac aagtttaaac
    62701 gcccgaccgt tatttaaata ttctgaataa tctgcttgca cattcgttga attttgtcct
    62761 ctgacaattt ccagttcttc ttcattcact aattgatact cctctccttt cttgaaatat
    62821 aggacatcct gcatagaaaa tcctgcatca tttgtgaaag gctgagataa ttttatcgtt
    62881 aagatccacg gatttttcgt ttctcgctga tcataaatcc ataatttttc ctgtatatcc
    62941 atttggccaa atgcttcttg tgtcaaacca gttgctttga tctgaccaaa atcgagtttg
    63001 gaaggggcaa gaaatgagag accaccttct actacgatct tcgtagcagg tgcaaaaggc
    63061 ggggcatcat gataaacaac tggcgtatca cttggattgt gattcccaat ttcatcgttg
    63121 gtacttggct tgttctgcac acccttagca gtagcttctc ctgcttggtc gtttaataca
    63181 atttctaata catctcctgc aaaaagttga ttttctggga tagaaagaac aaattcccct
    63241 gtatcatcaa tagttgtttc tgacaaaaca gcttgatttt ttttcaatga aaccaaggct
    63301 cctgcttcac ctgtacctgt gatcgtgcga gaagctgcat tgatcaatgt ggtacttaaa
    63361 ttagcaggtt caggaggaat cacatcgact gtcgttttgg cagagtccag ttctgtcact
    63421 tcttggccac ctcttacttt tttagcagca acaacttgca cttgatcctc cgtttttaat
    63481 ctctcatcat tgggtacttg aacttcgaac aagccgcctt gttcgccttc tccccatggg
    63541 ttatatcctg ccccttcatt ttcatctgtt tgtccttgcg tgagagcagt aatttctaca
    63601 gatgatttcg tcgtatctgc gtaagtaata cgtaaagtca cttctacttc gcctgtccaa
    63661 gcatctcttg gctttgcctc gacaccttcg ggaacaacga catgcccata gattttttta
    63721 tctgcattcg ttggcactct taaggattca atcatcggga gctgattatt ggcactgatt
    63781 cgattataat ttttcattcc ggcacctgtc tgagcaaatt catttgttaa acctgcacta
    63841 gggcttgata cgaaattgcc taagtcggtc ccattgatcg ataagtctgt catatcagaa
    63901 aaataatccg ggtctttttc aaagttatcg aatgttgcgc ctgtttttcg ccataaagcg
    63961 atttctgaat gggcgatctt taaagaagaa tctccatttc cttggaatac ccagccgcct
    64021 atgccattgg tctcgcttcc aatccgtttg ttttggaaat caaaataggt aggagattct
    64081 aagtccacat tcaacacatc cccactgatg atgccggtct tatcttcccc tgttttcccg
    64141 accatgacta aataagtttt ttcacctgcc gtaaaattta aatcaatatc tccagttgta
    64201 tctacagttg ctccggaatc tgcgcgtata tcgatatttg atttttctcc agtgactgta
    64261 aaatcagctg aacctggact ttctagaaaa ccagaagcgt cataaaattg gatcgcttga
    64321 tttccttgat tgagtctgtt cacgtaagat ttgccatcac ctgtatttct taccctcaat
    64381 gagcctccat tggttactgt cagcttttgc gtaccattct caaaacgaac tgcgctggaa
    64441 cgcccactct gcttatcgat gtcgataact gatccggctc cttctacgat caaagaaagg
    64501 ccttttccta caaaacgcat cccattgttc ccgggttgat cgtcatctcc agccattttg
    64561 accgagattt gtccaccact tgatacatgg atttgactag aatctccttg caagcgaaat
    64621 ccgcccgtag ttgctgtatt gatggtgatt ttcccaccat tcgatacagt gactttcgat
    64681 cctgaacctc tcatttggat gattccgcga ttgttgtcat tggtaatatt gatctttgta
    64741 tttaccgaaa attcgcttcc cgaaccactg acttcaaaca tgacattacc aggtagcgta
    64801 tcgactgcct ctccttcatc gctaacgcca tctgcccgat cataatcaag agagaccttt
    64861 gaaccgctta agatatggat tcctggcgct gtttttccac taccagtctc atctcctgaa
    64921 tctagaaaac cgtagaattt ccctagatac ggtccataaa gttgactacc attgatcaca
    64981 ttcaattgat agcttctaca aaaataggct ttacccggag cggcattcag tccttccttc
    65041 actggttgga catccaacac accggaaaca ccatctaatg tgacagttgc acgaggagcg
    65101 taaatcagtc ctaatttgga cccatcatac aaatcaagtg tcccggtaaa ttttatttct
    65161 cccgtaaaat gatatctgct tccccaaaaa tcggtcatat aagcaatgat aatggcattt
    65221 ccatcgatat ttggtgtcaa tccacctgga tgtgcagctg ttcctttaaa attctggaac
    65281 tcgatcgact gaggtatctc attgatataa atatggtgtg tgccgaaatt gtgagcgaat
    65341 ccaccaaaat caataacgat atctcgtttg atctctaatt ccctgtctaa agtcaaaggc
    65401 tgaatcagtc ggatcttagt tactgattcg tctgcgagaa gctgcgccag ttcttctgtt
    65461 gtggcagctt cgctgtaccc atccgctgaa acagctcttg ccagtgccgc ttcactgcta
    65521 tcagacgttt gttcagctac ttctgaggtc tcacttgtct tattttcttc ttgcctttta
    65581 ttagtagaag cctcactttg agattctgta gtttcagaag taatcgattc ttctgaaaaa
    65641 gtagtcttgt caaaactttg attctctgat tgcgttgatt cgtttgtttt ctctacatcg
    65701 gcaaaaacgg atgtcaccat caagagaggg aagacaaaca aaatcgacgc aatcagcttt
    65761 tttacacatt gattcattct ccaccatcct ctctgacgtt ttgacaattt tattttatcg
    65821 ttttaatcta tttccaattg tactttcata tttaaaatat gtatttttat ggaaaaattt
    65881 ttctattagg tgtaaatttc ttttttgtac aaaaaaaagg ctgtgacaaa agtcttgtca
    65941 cagtccctta tcccgaacaa gcggtgttct cccgccaact cctcggtcac aacctccatt
    66001 ttaacaataa acctgtaaat gtgagggcaa tactttgatt gtcacaggta atttatcccc
    66061 ttcatctcca tcaacattcg tcgtcaattc ttcctcatca gctaatgaca caactaattg
    66121 ctcacaagta aggtaggcta gatttttcgt agattcatct actcctttta ataggtctgg
    66181 caatgtttta agcgtatcga tcaaagaaga atcttttaaa taaaccaaat ggagcttacc
    66241 atcatccact tttgcatttg gaagtaatgt ttcaaaaccg ccgactgagt tagttagtcc
    66301 tatcagcaag gtactgctct caatttcttc cttctttcca tcaacttcta cgtgaaacga
    66361 gtaggattgt gtggaagcta actgtttgat tcctgacatg aaataagcta gttttccaaa
    66421 tttcgtcttc ttctccgcat ctacatcatt gattgattca gggatagagc caattgcgac
    66481 gacattcata aaatagtcgt catttatttt tccaatatcc aatggcttga ctgattcaat
    66541 cgaaaaatga ttgatcgctt cctcaggttc tagcggtatc cctaatgctc gagccaagtc
    66601 attcaccgta cccaatggga aaaatccgaa atttggccga tgttcttgtt cagcgattcc
    66661 actgattcct tcgttgactg ttccatctcc gcccatgaca aacacactat gatacccttc
    66721 cgtagccgct tcgcgagtaa agttttttgc atcccctgct ttttttgtat gcaagacgac
    66781 cacttcatca aacaccgatt ccaattttgc tatcgctaat tgctcaaatt cctttgcttg
    66841 ttcaccgcct gaactggggt ttaccactaa taaaactttt ttcatcactt tctttccctt
    66901 ctgattgatt tctttaatat aacgatagca taaagaaaaa aattaggctg tgatgaagta
    66961 ctgttcatga aacattcatt tattttttga caagataaaa catccatact atgaaactga
    67021 ggaaaattaa aacaatgata taggaaataa aagaaaatga taggcattgt atacctaata
    67081 aagtaaaaat cttaggtggc agataaaaac acaaaaatag caagagaatg taatagcgtt
    67141 tcttccactt tttgttatca gaccttatag cgatcagagg aataccaaat gcataactga
    67201 aaaactcttg atgtactgtt aatatgtcac ggccttgaat aataaatgcg acaatccccg
    67261 ctataattag tgtgcctaac accaaaacgg aaaattgatg aatatatttt atatacaaat
    67321 gatccattgt acttcctgcc tttttatttg tgagaaaaac aaatccgtat acgtgaataa
    67381 cttctagaaa taaaagaaat ttgcaaaacc actccattta tctacttcta cgtgcatttt
    67441 ttgatattgt tttctttatt taagattata cagaaaaata tctagggtcg ctggattcta
    67501 agtaaaaaaa atttttaaaa taaaaagaac taaatcaagt agtaattaaa ctactgttga
    67561 tttagttcta ataaaaagat ttagatgaac agttttattg atttaaatag ttcatatttt
    67621 ttatttatac cgcttattta ctacttgctg tactagaatc tgaagtgaat gtactcaaaa
    67681 cattttcaaa gtctttgtct ttgattttta cattagcgtc tttcaatact tcgccaatta
    67741 ctttattctg gaaggtactg tcacttaatt ttgtgtctgt tgcaatttct ttcaattctt
    67801 ttttgtattt atccatgtcg ttgcctttgt tttggttttt caccatttta accacataat
    67861 attccgtagt ataagttgaa gcattggtag aagtgatcac atcagaaact tgaccatctt
    67921 tcaacttaaa ggctgcttct ttgacttctg ccggaaccgt tgtagtagtg gaatcaaatt
    67981 tcacttttcc gccatcttct tttgtcgtag tatcagttga cttgtctttg gctaatttag
    68041 agaaatcgtc tcccttatca gcagctttct tcacatcttt tgcatcatct tcacttgcta
    68101 cttggataat ttgagcttcc acttctggat ggaaagattc ccaagccgtt tttaaatctt
    68161 catcggtcaa tttgatatgt ttctttagac cttcttgaaa ggctagactt tgtttgatac
    68221 tgtctttgaa tgtttgttct gtgtaacctg cagatttcaa ttgtgaatca aatgaatcac
    68281 ccaattgttt ctttgtttga tcatattgag aatcgatttc tttggttgat acgtcatctc
    68341 catatttatc ttcaaatact tgataaacga tcaactgttg cacagcggaa tctgctcctt
    68401 gcattttttt caagctatcc attacttctt gttcgctgat tgtaccgcct ttataactaa
    68461 cggctgtttt ttcaccggca ttcgtacagc ctgacaataa gccagtgcct agtacgatgg
    68521 caagtgctaa aaatcttttt ttcatttatc aattctccta cttttcgttt tttgcgaatt
    68581 gctaaaaaca aaggtgttca agatgaaaat catcaagaac acctttacta atctgctcga
    68641 tacgtcctga atagatataa ggctacacaa tccagaaagt tcgctcagca gaataaatgt
    68701 caagtagcac atcacacctt aatatactac ttcttttgtt accgtttttc aagtctatct
    68761 tgcgttccgt atttaacgta actaattctt agaacaaatg ttatcgattt tgtttttctt
    68821 cttccgctgc tgggatattc aaatcagaca cttttatgtt cgattttcca gacatttttt
    68881 tgttgttttt attatgatat atttttata
//
LOCUS       DAFBQH010000007        68851 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.007, whole genome shotgun sequence.
ACCESSION   DAFBQH010000007 DAFBQH010000000
VERSION     DAFBQH010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.007"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            303..545
                     /locus_tag="LK694_000500"
     CDS             303..545
                     /locus_tag="LK694_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBK5051396.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            542..1012
                     /locus_tag="LK694_000501"
     CDS             542..1012
                     /locus_tag="LK694_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBK5051397.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            1140..2354
                     /locus_tag="LK694_000502"
     CDS             1140..2354
                     /locus_tag="LK694_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBK5051398.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            3343..3552
                     /locus_tag="LK694_000503"
     CDS             3343..3552
                     /locus_tag="LK694_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HBK5051399.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            3664..5277
                     /locus_tag="LK694_000504"
     CDS             3664..5277
                     /locus_tag="LK694_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5051400.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTIFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            5312..6523
                     /locus_tag="LK694_000505"
                     /pseudo
     CDS             5312..6523
                     /locus_tag="LK694_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            6546..7472
                     /gene="glsA"
                     /locus_tag="LK694_000506"
     CDS             6546..7472
                     /gene="glsA"
                     /locus_tag="LK694_000506"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBK5051401.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            7680..9140
                     /locus_tag="LK694_000507"
     CDS             7680..9140
                     /locus_tag="LK694_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051402.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            9361..11124
                     /locus_tag="LK694_000508"
     CDS             9361..11124
                     /locus_tag="LK694_000508"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBK5051403.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            11208..11666
                     /locus_tag="LK694_000509"
     CDS             11208..11666
                     /locus_tag="LK694_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBK5051404.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            11775..12458
                     /locus_tag="LK694_000510"
     CDS             11775..12458
                     /locus_tag="LK694_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBK5051405.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            12605..13219
                     /locus_tag="LK694_000511"
     CDS             12605..13219
                     /locus_tag="LK694_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBK5051406.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            13235..13441
                     /locus_tag="LK694_000512"
     CDS             13235..13441
                     /locus_tag="LK694_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051407.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            13982..15112
                     /locus_tag="LK694_000513"
     CDS             13982..15112
                     /locus_tag="LK694_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBK5051408.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            15204..15959
                     /locus_tag="LK694_000514"
     CDS             15204..15959
                     /locus_tag="LK694_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBK5051409.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            16017..18056
                     /locus_tag="LK694_000515"
     CDS             16017..18056
                     /locus_tag="LK694_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBK5051410.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(18165..19316)
                     /locus_tag="LK694_000516"
     CDS             complement(18165..19316)
                     /locus_tag="LK694_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBK5051411.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(19470..20000)
                     /locus_tag="LK694_000517"
     CDS             complement(19470..20000)
                     /locus_tag="LK694_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBK5051412.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(20099..20449)
                     /locus_tag="LK694_000518"
     CDS             complement(20099..20449)
                     /locus_tag="LK694_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBK5051413.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(20606..22135)
                     /gene="ahpF"
                     /locus_tag="LK694_000519"
     CDS             complement(20606..22135)
                     /gene="ahpF"
                     /locus_tag="LK694_000519"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBK5051414.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(22145..22708)
                     /gene="ahpC"
                     /locus_tag="LK694_000520"
     CDS             complement(22145..22708)
                     /gene="ahpC"
                     /locus_tag="LK694_000520"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBK5051415.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(22852..23514)
                     /locus_tag="LK694_000521"
     CDS             complement(22852..23514)
                     /locus_tag="LK694_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5051416.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            23698..25344
                     /locus_tag="LK694_000522"
     CDS             23698..25344
                     /locus_tag="LK694_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBK5051417.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            25335..26417
                     /gene="uxuA"
                     /locus_tag="LK694_000523"
     CDS             25335..26417
                     /gene="uxuA"
                     /locus_tag="LK694_000523"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBK5051418.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(26525..28078)
                     /locus_tag="LK694_000524"
                     /pseudo
     CDS             complement(26525..28078)
                     /locus_tag="LK694_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            28226..29367
                     /locus_tag="LK694_000525"
                     /pseudo
     CDS             28226..29367
                     /locus_tag="LK694_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(29493..30572)
                     /locus_tag="LK694_000526"
     CDS             complement(29493..30572)
                     /locus_tag="LK694_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK5051419.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(30675..31115)
                     /locus_tag="LK694_000527"
     CDS             complement(30675..31115)
                     /locus_tag="LK694_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK5051420.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(31172..31582)
                     /locus_tag="LK694_000528"
     CDS             complement(31172..31582)
                     /locus_tag="LK694_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBK5051421.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(31762..32196)
                     /locus_tag="LK694_000529"
     CDS             complement(31762..32196)
                     /locus_tag="LK694_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK5051422.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(32296..33144)
                     /locus_tag="LK694_000530"
     CDS             complement(32296..33144)
                     /locus_tag="LK694_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK5051423.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            33533..35299
                     /locus_tag="LK694_000531"
     CDS             33533..35299
                     /locus_tag="LK694_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBK5051424.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            35401..36660
                     /locus_tag="LK694_000532"
     CDS             35401..36660
                     /locus_tag="LK694_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK5051425.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            36807..38108
                     /locus_tag="LK694_000533"
     CDS             36807..38108
                     /locus_tag="LK694_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5051426.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            38111..38962
                     /locus_tag="LK694_000534"
     CDS             38111..38962
                     /locus_tag="LK694_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5051427.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            38975..39790
                     /locus_tag="LK694_000535"
     CDS             38975..39790
                     /locus_tag="LK694_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBK5051428.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            39941..41107
                     /locus_tag="LK694_000536"
     CDS             39941..41107
                     /locus_tag="LK694_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK5051429.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPTQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            41337..41984
                     /locus_tag="LK694_000537"
     CDS             41337..41984
                     /locus_tag="LK694_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBK5051430.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            42068..43012
                     /locus_tag="LK694_000538"
     CDS             42068..43012
                     /locus_tag="LK694_000538"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBK5051431.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            43147..44598
                     /locus_tag="LK694_000539"
     CDS             43147..44598
                     /locus_tag="LK694_000539"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBK5051432.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            44619..45455
                     /locus_tag="LK694_000540"
     CDS             44619..45455
                     /locus_tag="LK694_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBK5051433.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            45455..46114
                     /locus_tag="LK694_000541"
     CDS             45455..46114
                     /locus_tag="LK694_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK5051434.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            46240..46929
                     /locus_tag="LK694_000542"
     CDS             46240..46929
                     /locus_tag="LK694_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051435.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            46935..47891
                     /locus_tag="LK694_000543"
     CDS             46935..47891
                     /locus_tag="LK694_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBK5051436.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            47935..48447
                     /locus_tag="LK694_000544"
     CDS             47935..48447
                     /locus_tag="LK694_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5051437.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(48703..49251)
                     /locus_tag="LK694_000545"
     CDS             complement(48703..49251)
                     /locus_tag="LK694_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK5051438.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(49340..50521)
                     /locus_tag="LK694_000546"
     CDS             complement(49340..50521)
                     /locus_tag="LK694_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBK5051439.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(50757..52265)
                     /locus_tag="LK694_000547"
     CDS             complement(50757..52265)
                     /locus_tag="LK694_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBK5051440.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            52424..52615
                     /locus_tag="LK694_000548"
     CDS             52424..52615
                     /locus_tag="LK694_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051441.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            52818..53735
                     /locus_tag="LK694_000549"
     CDS             52818..53735
                     /locus_tag="LK694_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBK5051442.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            53728..54801
                     /locus_tag="LK694_000550"
     CDS             53728..54801
                     /locus_tag="LK694_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051443.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            54949..55467
                     /locus_tag="LK694_000551"
     CDS             54949..55467
                     /locus_tag="LK694_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051444.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            55448..55840
                     /locus_tag="LK694_000552"
     CDS             55448..55840
                     /locus_tag="LK694_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBK5051445.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            55916..56851
                     /locus_tag="LK694_000553"
     CDS             55916..56851
                     /locus_tag="LK694_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBK5051446.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            56984..57763
                     /locus_tag="LK694_000554"
     CDS             56984..57763
                     /locus_tag="LK694_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBK5051447.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            57756..58073
                     /locus_tag="LK694_000555"
     CDS             57756..58073
                     /locus_tag="LK694_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBK5051448.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            58317..59927
                     /locus_tag="LK694_000556"
     CDS             58317..59927
                     /locus_tag="LK694_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK5051449.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            60084..60932
                     /locus_tag="LK694_000557"
     CDS             60084..60932
                     /locus_tag="LK694_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK5051450.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            61163..61642
                     /locus_tag="LK694_000558"
     CDS             61163..61642
                     /locus_tag="LK694_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051451.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            61675..63903
                     /locus_tag="LK694_000559"
     CDS             61675..63903
                     /locus_tag="LK694_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK5051452.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            64032..65675
                     /locus_tag="LK694_000560"
     CDS             64032..65675
                     /locus_tag="LK694_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBK5051453.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(65748..66293)
                     /locus_tag="LK694_000561"
     CDS             complement(65748..66293)
                     /locus_tag="LK694_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBK5051454.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(66316..66801)
                     /locus_tag="LK694_000562"
     CDS             complement(66316..66801)
                     /locus_tag="LK694_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK5051455.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(66791..67603)
                     /locus_tag="LK694_000563"
     CDS             complement(66791..67603)
                     /locus_tag="LK694_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBK5051456.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(67742..>68851)
                     /locus_tag="LK694_000564"
     CDS             complement(67742..>68851)
                     /locus_tag="LK694_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBK5051457.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        23047 a        12160 c        13527 g        20117 t
ORIGIN      
        1 ctaaaataaa aatggggctg aagaatcaat tctgattcat cagtcccata aattatagag
       61 ccaaaaaata agaaattgat tgtcgttttc ttttgcagaa atttaagtgc aggcaattat
      121 ggtagttgtc gtaattgctg tcagctcagt gtttatcact gatggtgtga aagatttacg
      181 gttggagatg ttcaataaga ttattggtgc ttaattattt ttattgtagt gctgatatga
      241 gaatgttata attggggtat aaaacataca aataaatttg ctgaaaaatg aggtatcaaa
      301 atatggaaaa tgaattactg gaaaacttag gaagacttca cacaactgaa cttggcgtta
      361 ttagaattaa aaagaactta tctttgaatg tagaaaatgt aattgaatgg tgcaaagaaa
      421 aaattagcct ttctaatgcg gaaatcacaa gaaaaggaaa aaactggtat ataacgattg
      481 ataattgtat tataacggta aatgcgtaca gttataccgt tatcactgct cacaaggtaa
      541 aatgataaat ttttctataa aggaattaaa cgcatttgca gttattgggc aggaagtaga
      601 actgactaat tatcaaaaaa agaatattca gattagtaca cagttttgga gaaagtttaa
      661 cagtagtttg aaaaaagcgt atctttcaca atcaggaaat tgggtaaaat atgcttttat
      721 ggaaagaaga aatggaaaac tttattattt ctgttctatt ccccaaagaa ccattacccc
      781 agagggcttt ctgtataaag aaataccgtc ttataaatat ttggttgtgg agcatattgg
      841 tgctatggat aaaatatatg aaacttatgg caagatttat gaagaaatta ttcctagtac
      901 atcgtatatt cctataaaag atattatcct acactttgaa aaatacgatt atcgttttca
      961 ttggaatagt gataattcag ttattgaaat ttggatacct attcaagatt agaagcaatt
     1021 ttatatccat acacacaaag cagttagtct taggattgac tgctttttct atacagattt
     1081 ttaatgacag gcaattatca attatggtag ttgccttttt tgattggagg aattgaagaa
     1141 tgaatgataa aaactttatt gaagaactaa gacaaaagcg tgaggaatac ggagtaacac
     1201 aaacaaggct tgctgttgcc tgtggtatca gccgtgaata ttacaaccgc attgaaaaag
     1261 gaaaacagcc attgaatgac gaattaagag aaatcataga aaaacagatt gagcgtttca
     1321 atccgcaaga accactattc ttattgattg attactttcg tgtccgcttt cctactacgg
     1381 acgcattggc gattatccgt gatgtattac aactgaaatc taattacatg ctttatgaag
     1441 attatggaaa atacggatat gaaagcaaat atgtacttgg cgacatcaat atcatgtgtt
     1501 ccatgcagga gcatttaggt gttttattgg aactgaaagg caaaggctgt cggcaaatgg
     1561 aatgttatct gctggcacag gaacgctcat ggtatgactt catgctggat tgtatgacgg
     1621 ctggtggtgt gatgaaacgg cttgatttgg ctatcaatga taaagcagga atattggata
     1681 ttccaaagtt aaaggaaaaa tacaaggctg gcgaatgtat gtcctacttc cgtatgcaga
     1741 aagattacag cggtacagaa aaatgcggta gcgatttacc aaagaataca ggagaaacct
     1801 tatatctcgg ttcaacaagt agcgaattat atatgtgtgc ttatcagaaa aattatgagc
     1861 agtatgtcaa gaatggcaca gaaattgaag atacggagat taagaaccgt tttgaaatac
     1921 gcatgaagaa cgaacgagcc tattatgcgg ttgtagattt actgacctat cgggacgctg
     1981 aacgcaccgc tttttctatc atcaatcatt atgtccgctt tgttgacaga gaggacgaca
     2041 agccaaaaag tcaatggata acaaatgatg attgggcatg gtttgtaggg gaaaacagag
     2101 agccgatacg cttaaccaca aagccagagc cttacacttt acaaaaagcg ttgcattggc
     2161 tacaaagaca agtcgcacca accataaaaa tggtacaggc attagacaga gaaaatcgta
     2221 caacgatact gaaagatatg attgagcagg cagaactaaa agacaagcat aaacatttat
     2281 tacaattaga gaaatcaacc atagaagaac gtatagatac cgctgttcca caagagaatg
     2341 acggtatttt ttaatgtaat tgaaaaattt cagatttagt cagcaaagag ctgttttcat
     2401 tccctatgtg aagtgaaaga agaaaaataa attttttatt aaatctgcaa caaaatggct
     2461 acttgcgtac agatatggtg cgagaaaagg aaatctaaac tttttttgaa aataggtcat
     2521 taaatcgggg cttgctgtcc ctatatgaag tgaaagaaga aaaaagtttt gaaaaaatga
     2581 cggaaattcg tgattagtgg acagtcttct gtgaaaagag gaaaaacatc atcattttgt
     2641 attcaaacag gcattagcag ttgagtgttg atgtgaaaag atgaaaatac tttgttctca
     2701 acttaacaat ttaaggtttc cgttccctat atggtgcaaa gaaaatattt cattctattg
     2761 ttggcagtaa cggtatggtt tgggtagtta gggtgtgaaa aggaaaatat tcttttttca
     2821 tcatcaactt agcaaaatga cattttctat ccctatatgg tgcatgaaaa gaagttaaaa
     2881 tttttccatt taaacttagc aaatggacgt ttgctgtaca gatagtagtg aaaaaggaga
     2941 aaatcaaccg ttgattgcca aaatgaaccg ttccatttgt actaataatg gaaaaagttt
     3001 tggattttgg ttacgtttct cctcttttcc aacgcgatat ataggaaaga cgattttttt
     3061 ctttcaatcg ttctttgaca actgaataaa gctattcaat acgtttgaca gacagctagc
     3121 cgttgaaaca aatacgccac gacctttccc ctgcaagagc gagcgaaaac ctatttgtgt
     3181 tcttgtaaaa gatttgtcct cttttatcgc catgacctgt actgtcagat aatgatactc
     3241 ccgtacagcc acagcttgag cattaagagc gtcgcacgca atgggtacgg ctacataaga
     3301 actatgcaga ggtggaactc ctgtgggcta tgacaaaagc cgttgaattg cttagaaaga
     3361 ttttacagaa agggggtatg tgttattgat aatgctgtaa aaacaattca aaagaaaaat
     3421 ggcaagtgtg gcattggcaa cagaggaaag acaaagattg tagtaaaaga gcatttttcc
     3481 gaaaagggca aaacaatgga agaacttcta actgatgtca tgctggaaaa agcaaagcag
     3541 acaatcgcat aaaatcggtg cagttgtaag aaatagattg aatgacaaaa agtacccgtg
     3601 ttatacttta cttgcaagca ggtattgtaa acacgggtta gttataaagg aggataactg
     3661 acaatgaaac aacagattta caatactgca ctttatctta ggttaagccg agatgatgaa
     3721 ttacagggcg aaagttccag cattaccaca cagagaagta tgttgcgttt atatgcaaaa
     3781 gaacatcatt tgaatgtcat tgatgaatat attgatgacg gctggtcggg aacaattttt
     3841 gacagaccga gttttcaaag aatgattgag gatatagagg caggaaaaat caactgtgtt
     3901 gtaacgaaag acctttcccg tcttggcaga aattatatta tgacaggaca atatacagaa
     3961 ttgtatttcc ctagtcataa tgtccgctac atagcgattg atgacggtgt agacagcgaa
     4021 aaaggcgaaa gtgagattgc accatttaag aacatcatca atgaatgggt ggcaagagat
     4081 acaagccgta aagtcaaatc agcatttaag acaaagtttg cggagggggc tcattacggt
     4141 gcttatgctc cgttaggata taagaaacac cctgacatca aaggaaaact gttggttgat
     4201 gatgaaacaa aatggattat tgaaaaaatc ttttccctag cctatcaagg ttacggaagt
     4261 gcaaaaatca caaagcagtt aagagcagaa aaagttccga cagcgtcatg gctaaatttt
     4321 acaagatacg gtacttttgc acatatcttt gagggaaaac ccgaaagtaa gcgttatgag
     4381 tggacgattg ctcatgtcaa ggcgatattg aaaagtgagg tttatatcgg aaacagtgtt
     4441 cacaatatgc agtctacggt atcgttcaag agcaaaaaga aagtgcgtaa gcccgaaagt
     4501 gaatggtttc gagtagaaaa cactcacgag ccgattattg acaaggaagt gttctatcgt
     4561 gtgcaggagc agataaaatc aagacgcaga cagacaaagg aaaaggcaac gccgattttt
     4621 gcagggcttg tcaagtgtgc ggattgtggt tggtctatga gatttggaac gaataagaca
     4681 aataaaacgc cttacagtta ttatgcttgc agttactacg ggcagtttgg caaaggtaat
     4741 tgttctatgc actacattcg ttatgatgtg ctgtatcaag ccgtattgga acgcttgcag
     4801 tattgggcta aggcagtaca gcaggacgaa gaaaaggtat tgaacaagat acagaaagct
     4861 ggcaatgcag agagaatacg ggaaaagaag aaaaaggcaa gtacattgaa gaaagccgag
     4921 aacagacaaa atgagattga ccgtttattt gcgaaaatgt atgaagatag agcctgtgag
     4981 aagataacag agcgaaattt tgtgatgttg tccagcaagt accaaaaaga acagatagaa
     5041 ctggaacagc agataacaag cctaagagaa gaactaagta aaatggaaca ggatatgata
     5101 ggtgctgaaa agtggattga gttaatcaag gaatattccg taccaaagga actgacagca
     5161 ccattattaa atgccatgat agaaaaaatc ctcatacatg aagcaacaac gaatgaggat
     5221 aacgaaagaa tacaggaaat tgagatatat taccgattta ttggaagagt tgagtaatca
     5281 acaagagtaa tatttttaac taagggaaac cggcggtgtc tatacttggg tcagtcgcac
     5341 attgggaaaa cgattcggtt ttgccgcaat ctttttccaa tggtttgaga taacagttgg
     5401 ctacttgacc atgctgtatt tcttaactgg tgcattatcc tatgcaacgg gtattggtgc
     5461 tatacaaaac aataaatatc tgaaattggc aatcctattg attattttct gggctatttt
     5521 actctcacag ttaaaaggaa ccaaatacac ttcattgatt gctcggatcg gttttgtagc
     5581 tggtatcttg ttgccagcat tagtcttgtt tggcttaggg atccattatg tcgcttcagg
     5641 cgctccacta caaacgatac tgtctatgaa aacattgatt cctgattttt cgaaattgcc
     5701 tacgctggtt gtttttgttt cctttatttt agcatatatg ggcgtagaaa catctgccag
     5761 tcatgcaaat gaaatggaaa accctaaaaa ggattatcca ttagcaatgt ttgtattggt
     5821 tattctggca atcattttag atacttttgg cggattgacc gtcgctacta cagtacctca
     5881 acatggtttg tctcttaata caggggtgat tcaatcgtta gaattcctat taagacacat
     5941 tgatccaaat cttgcatgga ttgcgaaaat tcttgcgatt cttgtttgtt taggtgtcat
     6001 cggagaaatt gcttcttggg taacctctcc ttcaaaagca cttcacgtag cagcagaaga
     6061 aggattactt cctgaatatt ttgcaaaaga aaacacacac ggtgttcctg ctcatttgat
     6121 gatcgcaaat gggatcgttg caacaatctg ggcagccgtc tttacattaa gcggcggggg
     6181 gaataatctt tcatttcttg cagcgatgtc attgactgtt gtaatctacc tgatgttgta
     6241 ctttatgttt ttcgcgggct atttgaagct ggtttttaaa gatgacagtc ttgaaagagc
     6301 ctatcaggtt ccaggcggaa aagttgtaaa agtaatcgtt gcttgtttag gtcttttgac
     6361 ttcacttttt gctttttgta tctcatttgt tccacctgct agtatcgcag ctggacagca
     6421 tgggatttat gaaacgattc tgattgtttc ttatcttatc gtcttagtta ttccatttac
     6481 gatctacgca tgccgtcata aattccagca tgcagaaaaa taaaaaatga aagctggtta
     6541 ttcaaatgga agtaaaagaa ttagaacaat tgattggaaa aaatttccac tattatcaaa
     6601 caggacaagt ggcggattac attccagcat tagctgaagt ggatcccaaa caattaggta
     6661 tggcgattta tgatttggat acaaaacaac tgattgaggg aggagacgcc gatgtgcgct
     6721 ttgccatcga aagcatgtca aaagttcccg tattgttatt agcaatcaaa gacaacggaa
     6781 tagaaaaagt ctttcaaaca atcaacacgg aaccaaccgg ttttgccttc aattctatca
     6841 tgaatatgga aatcaatcat cgcaaacatc caatgaatcc ttttgttaat gcaggagcga
     6901 ttgcaacaac ctcgttgatt gccggtaaag atgcagatga aaaatttgag cgaatcttgt
     6961 catttatgaa agagatctgt gatgatccta atatcacatt gaatgaagaa atctatcatt
     7021 ctgaatcaag aacaggtgat atcaatcgct ctcttgctta ctatatgaaa ggtaatcaaa
     7081 tgatcgaagg agacgtaccg gagatactcg atgcttattt cagacaatgc tccctcaatg
     7141 tcaccgctac aggcatagca aaattagctg ctgtgctggc aaataaagga attgctcctt
     7201 ggaatggcaa acaaatcatt actgaagaaa gtgctacaat cgtcaaatca attatgacaa
     7261 ctgctggttt gtatgatgaa tcaggagagt tttccgtcca tgttggtgta cctgcaaaaa
     7321 gcggtgtagg cggcgggttg atggcagctg tgccaaatcg ttacggcata ggtgtcttca
     7381 gcccggcact agatccattt ggtaacagcg cagctggtat ccaattgttg aaagatgtag
     7441 tcaaagaact agacgctgac atctttgaat aaccattctt gaaagaggct ggggtaaaag
     7501 tccaaacggc tcaaagctac ctacaatgag gctgaaattg tgagcttgtt tgacttttgt
     7561 cccagtttct ttttttattg gtttttgaaa gggcttttct aaataaaaag gaaacaaaaa
     7621 tacgatttac agatatgatt aaataaaatc ataagaaaaa ctggaggcgt gtatcaggaa
     7681 tgctggaaaa ttacatcgag cgaaatatat ttagaaaagt gtatctgtgt gaacaattat
     7741 ttgaattcca agaaatagac atcgaacaaa cagctatttc tctaagggtg actacaccaa
     7801 cgatccttca cgatctagaa agtctagccg agtgtctaga atattgtata aaagaacaag
     7861 taagagagaa gcataagtat aaactcgtat tcaaacatgg tattgctctt tctgagctca
     7921 cacagtttct gtacggtcaa tcttattttt taaagttttt aagttattat tttaatggta
     7981 tattcacgag tacagaactg gctgatcttg aatttatttc tttgagtaaa gtatatacaa
     8041 tcaaaaaaat cgtattggat ttttttaaag aaaatgatta tttgaaaaat aaagaaatca
     8101 ttatcccaga atttgactcg cggaacatct tgctagcttt agtacgttat gtcaattggg
     8161 aaggctatga aaataaaaat ccgtatgtag aagaagcttg taacgagttg attgattatg
     8221 tagagactca tttttttaaa agaagatatt cggaagatga aaagtccttt atcatacgag
     8281 aaatagagat cgcaatcgga agaaaggcat ttccccttgg actgaaagaa gaagataaga
     8341 aaagcgcaga aacgaagccg ttatttcaat tagtctgtca aggattggaa gagcaaaaag
     8401 gaattctaga cttacaagaa gaagatgtct attatatttt ttctttgttc aacagtagaa
     8461 attataccaa tgagaacatg gagctgttaa gaaaagattt cgaagttgtt tataaaaatt
     8521 ttgttctcaa tgacctatca ctgaatgaat tgattttaca gattattagt atcgctgaaa
     8581 tcgacgatgc agaaaatttt attttcaagc aatccttttt acaatttgtc cgtacgttgt
     8641 gggcagatgg acaagtattt ttaccggaaa ggatctatct tctatcagaa aaagaacaac
     8701 ttctgtacgg aaaaattgta gaaatattgg aagagtggaa aaagaaaaat caaatccaac
     8761 taagatggaa tgaaaatttc attcgaaaat tcgtcaaaag tctttctttg atcaacagtg
     8821 aacaagcggg gagacaggaa atagaaattt ttgttgtttc agaaagttca gtcaaacaat
     8881 ttttttatcg aacccaattt catttgtatg cggaagaagg catcgctgaa ataaatccta
     8941 ttttatttcg ttcattagaa gaattgcctg atgaatgtct atgtgcgaaa aaaagagtgg
     9001 ttttgtgcga tgctgctagc tatcaaaatg gattagaaac tgaaaagaca aagatctatc
     9061 caatctcatt aaaaggtgca aacatccatt tgtatcatat tgctcaggaa cttcacctat
     9121 taaaaaaaca tatttcttga tgagacctta attaggagac cttctacaaa gctttttaat
     9181 tgtctgtaga aggtttttat ttgagtcctg attcgtgcga agatctactt atataacaag
     9241 ctttctctga aattttttta aaatgctatt gacttagagt caactctaag tagtagaata
     9301 gataaagtgt aacaaatcta ttttcattaa aagaagatag tacggaggag agaatgtatt
     9361 atgagtacag tattacatgt attggatgca atcaatagcc ccgcagatgt caaaaaatta
     9421 tctgttgagg aaatgaaaca gttggcagaa gagatgcgcc aaaaaatctt gcaaaaggac
     9481 agcgatgtag gtgggcacgt tggtccgaat ctaggtgtag tagaattaac gattgcgttt
     9541 cattatgtat ttgattcacc aaaggataaa atcgtttggg atgtttccca tcagtcttat
     9601 ggtcataagc ttttaactgg tcgaaaaaat ggttttgaaa aaggacatta ccgtgaagtg
     9661 agcggatatt ccaaccaaca tgaaagtaaa catgactatt ttacagtggg gcacacatct
     9721 acatctatca gcttagcaac aggtttagct aaagcgagag atctgaagaa agaatcagga
     9781 aatgtggtag catttatcgg tgacggctcc ctaagtggtg gcttagcttt tgaaggattg
     9841 aacaatggtg gtgttttaca atccaatcta atcgtattag taaacgataa tgagatgtct
     9901 attgatgaga atcatggcag tttatataaa aacttagctg agcttcgtca aaccaacggt
     9961 aaaagcagca ataacctatt taaagcaatg ggatttgatt atcgttacgt ggataatgga
    10021 aacgatctgg aaacggtgat ccgtttattt gaagaagtaa aagatattga tcatccgatt
    10081 gtacttcacg tccatacgga aaaaggtcgc ggttatcagc cggcaatcga taataaaatg
    10141 aaatatcatt ggcatgtacc atttgatctg tccaccgggg aggcaaagca agtaacttct
    10201 ggagaaacat ttaatatgat catcatggat tatttggata agaaaatgca aacaagtaca
    10261 gctcctattg ccgcagtcaa tgcagcgata ccaggcgcct ttggactgaa aacattccaa
    10321 gaaaaacatc cagatcatta ttttgacgtg gggatcgctg aacagcactc tatcactttc
    10381 gcttctgctt tggcttctca gggagtacgt ccagtcgtct tccaatcaag tactttcttg
    10441 caacgggcat atgatcaatt atcacatgat ttagcgatca acgagaatcc agtgattatt
    10501 ttagtcggtg gcggaacgat ctcagctagt gaccgaacac accaaggaac atttgacatt
    10561 ccttacttat caagtatccc gaacatcaaa tatcttgctc cagcaacaaa agaggatatg
    10621 atctctatgc tagattgggc gttcgaacaa acagcgcagc ctgtagcaat ccgaattcct
    10681 acacatggtg tagaacatgg tacagctatc gcaaatgatt atagtattcc agcctatcaa
    10741 acagtaaata atggaggaaa agtagcaatt ttagctttag gcggttttct tgaattagga
    10801 aaagaaacag taaaagcttt tagaaaaaaa ggaatcgaag caacgttgat caatccagga
    10861 acgatcacag aaaatgacgt agaaacatta gataaattaa aagaaacaca cgaattagtc
    10921 atcacattag aagatggtag tttgtcagga ggatttggcg aaaatatcag tcgttattat
    10981 ggtgattctt caatgaacgt attaaatttt ggtgcagaga aagaattcac ggatagcgtt
    11041 cctgtagggc agctttatga acgttatcat ttaacaccaa aacaaatcgt tgaagatagt
    11101 ctgcgtgtct tgaatattca ataattcatt atgctcacca tccagataat tgtttatact
    11161 agtatctgga tggttttttc attataaaaa gcaaaggggt ggatgttatg tatcagcaag
    11221 tagaagaata tgtcaaaaag ttccaagaaa aatatcctag ttatcaatca gaaaaattag
    11281 gtttcttttc atttggggat acagaaaaaa tggcagacga gttgagcgaa ctagttgtaa
    11341 aaaagataaa gactggtaca tcaagtggtt acgatctgta taaacaagag aatgagcctt
    11401 taccaaaagt tggtcaatta gacgtggtcc tgaatggaaa aaatgaacca gtttgtatca
    11461 tcaaaaatat aaatgtgacc atcttgcctt ttagtgaagt cacgaaagag catgcttgga
    11521 aagaaggaga aggggatcgg acattagctt attggcggaa agtccatgct gagtttttta
    11581 aacatgctta taaagaagcg ttgaacatag aatttacaga agagaagctg gtcgtatacg
    11641 aagaatttga agtaattttc ttttagtaag aaaggtctgc tgaaagaaat agctcattat
    11701 tgtctcctga atagaaaagg gttacatttt tatatgaagc tgtattttta tataacatta
    11761 ctggaggaga tattgtggat acactggaac aaatcaaagg cggagtcgtc gtttcttgtc
    11821 aagctttgga aaacgagccg ctacacagtt cttttattat ggggagaatg gctttggctg
    11881 caaaagaagg cggagcagtt ggtattcgag caaatacgac agaagatatc aatgaaatca
    11941 agcgaatcgt tgatttaccg gtaataggga ttatcaaaag agattacgat gggtcagaag
    12001 tgtttattac tgcgacagaa aaagaagtgg aagaactttt ggagacgcaa gcagaaatca
    12061 ttgcactgga tgcaacttct cgtaaacgtc cgacagaaaa aacaactgct gaacttgtcc
    12121 aaatgattca cgaacacggt cggttagcta tggcagatat ttcgactttt gaggaagcgc
    12181 ttcaagcgca agaagatggt tttgatcttt tgtctacgac tttagcaggc tatacagaat
    12241 attcacgaaa gacgcagaca cctgattttg atttattgaa tcaaatcata gaacaagtcg
    12301 atatcccagt aattatggaa ggacatactg actctcctga acaagtagag aaagcatatg
    12361 aattaggtgc tttttcagtc gtagttggat ctattatcac ccgtccgcaa ttgatcacta
    12421 aaagatatgt cgaagccgca agtaaagcaa ataactaaaa aacacgcacg gcacagattc
    12481 ttgacaaaaa agaaaaattg atagatagtt taaagcagat tctaagaaga tgaaaaaagc
    12541 attacttttc attttgtctt caagatctgc tttttaacga attttataga aagaaggcga
    12601 aaccatgttt gaacggagtc cttcagaatt gacagaacgt gaaaattaca agttgctgat
    12661 tggttcagtc atccctagac cagtagcagt agtaacgact aaatcagcaa aagatatagt
    12721 gaatatcgca cccttcagtt acttcaatat cgtcagttcg aatcgaccca tcttatcctt
    12781 ggcgatccag agaaaacaag atgcagtaaa agacacagca aagaatattc ttgagacaag
    12841 agaagctgtt atccatatcc ttgatgagga caacgtagaa gaagtaaata aaacagcagc
    12901 caatctgcct aatgaggaaa gcgaactgta cctgactgac ttgactttaa ctgattcagt
    12961 aatcatttca gtaccagcat tgacagaagc aaaagttcgt tttgaaacat ccttatatga
    13021 acatgtcgag atacggaata aaaataaagt aacagctgat ttatttttgc tagaagtaca
    13081 gaaatatcat ttaaatgaaa cagtctatga tgataagaca ggaaggatcg atccaagaaa
    13141 attgaatgct gtaagccgtt tggcaggaaa tgattatgca gcaataggag aaattttcac
    13201 gattgcacgt cccgaataaa aaggagcact agttatgtta aaaataagct ataaaccatc
    13261 gacagattca aaagaaatga aaaaagaata tgaaacagtg aatgattttc tgcaagggca
    13321 gtacttagaa gttccgccat tgcaagacca ttttgtcgtt actactgtta ctttagatgg
    13381 taaggaaata gaaatgccag atcaaacgat tagcggattg tttaattact ttaacaaata
    13441 aatatttatt ccgaaaagtg aaaaaacata taaaaaaaca cataaaacta aaaaattatc
    13501 aaagaatcct ggtgcaactg aaagaatttg caacgggatt ctttcaactt atttgttatt
    13561 tttttctaaa aaatattaaa gattgttaaa tttcactatt tcatttcaaa ttccattgcc
    13621 taatcattag gaaaagtcta tcatgaactc gtaaatagaa tatagtaata accttgttag
    13681 gtgaggctcc tatacaaaca taggctgttg cccagaaacg tcgaaagtcg ccaatgggta
    13741 aaacagaaac tgttggatta aggctttttc caatagctaa gaatgttttc tttacgttgt
    13801 atagtgctaa aactcggacg atgagatgat gctaggtcag aattcttgtt cgaaaaggtt
    13861 tctgaccttt tctttatatt tttgtgactg acttatttga tgagacaaac tcgctcctca
    13921 cctgacaagt ggattgctgt gtgaggagga gatgcggtga aaaaagatcg attggtggat
    13981 tttgggtcct tatttgactt tgtccatgat cggtctttta gaagtataca gtgccagttc
    14041 ttatcgttta ttgcaagccg atgaaaatac aaaaagtcta ttattgcgtc aactaatttt
    14101 catatttttg agttggagcg tgatcttctt agctcgttca gtcaaactac actatttact
    14161 tcaccctaaa atagcaggat acggtttagc cttatcgatt ttctttttag tattagtaag
    14221 aatagggata ttcggtgtca ctgtcaacgg cgcacaacgt tggatctctc tgtttggcat
    14281 tcaattccag ccttctgaac tggcaaatct ttttttgatt ttttatttaa gctggttttt
    14341 tcgtgacgga aatagtagcc caaaagatct aaaaaaacca ttcctgatta cagtaggtat
    14401 aacttttctg attttatttc agccaaagat tgctggagca ttgatgatcc tttcgattgc
    14461 gtgggtcata ttttgggcag cggcggttcc atttaaaaaa gggatctatc taatcgttac
    14521 tttttctgca ttgctgattg gagcagcagg cggggtatta tatttaggaa ataaaggttg
    14581 gcttccacag atgtttaatc atgcttatga aaggatcgcg actttaagag attcttttat
    14641 cgatagtcat ggtgcgggct atcaaatgac ccattcgttt tatgcactat ataatggtgg
    14701 catatttggg cggggattag gaaatagtat cacaaaaaaa gggtatcttc cagagacaga
    14761 aacagatttt attttttcga ttattacaga agagcttggg ctaatcggtg cattgtgtgt
    14821 gcttttctta ttgttctcat tatgcatgag aatcttttgt ttaagcagtc gctgtaaaaa
    14881 tcagcaagct ggattgtttc ttttaggatt cggaactttg ctattcgttc agacgataat
    14941 gaacgtggga agtatcgcgg ggctgatgcc aatgactggt gtgccattgc catttgttag
    15001 ctacggagga acaagttatc tgatcctttc tttaggaatc ggaatcacgt tgaatatttc
    15061 ttcaaagata caggccgaag agttggcgct ctatcgacct gaaaaacaat aaagagaaac
    15121 tgagaaactc tcagtttgaa ttatcataaa gtacaatctt ttatttttca agcgagaggt
    15181 tcttttagaa agaggaattc gcaatgaaag tatctcaaaa aatcatatta tctattttgt
    15241 tactgcttac attagtagtt ggctattttt cgattgagtt tatccatgga ttttcgtctg
    15301 caaaacagag ttcaactgtg aaaaaggtag agccgaaaag tatccctacc acactaaatg
    15361 tggctttgat tggttcagat gctcgttcga aagacgaaaa cggtcgctcg gattcacttc
    15421 tggttgcaca atacaaccag aaaacacaac aagcaaaact gatctctatc atgagagatt
    15481 catatgtcga tattccaggt tacggaatga ataagataaa tgcagcttac tcttatgggg
    15541 gagttgattt attaaatcaa acattaaagg aaaatttcaa atttgaagct ccctattatg
    15601 caagtatcac atttcaagat tttatcgatt gcgttaatga actgtttcct ggtggagtca
    15661 agatcgatgc agaaaaatct ctagatttag atggcgtata tataaacaaa ggagagcaag
    15721 tgatggatgg taatacttta ctccagtatg ctcgatttcg ggaagacaaa gaaggagact
    15781 tcgggaggat cagaaggcag cagcaagtga tcaaagctat ctctcagcag ctgaaagacg
    15841 taacttcaat attcaagcta cccaaagcgg ttgggaaatt acttggtagt atccaaacga
    15901 accttccaga aagtgtgctt ctggattgtg gtatggattt cctcaaagac atatgttaga
    15961 ataaacaggt ataaatagtg aaataaagga atgacaagca agagaaggag gaaaaaatga
    16021 aaagaagtga caagcacggc aaaaatcgaa caggcgctta tattgccggc gcagtgatct
    16081 taatagcagc tgcgggtggc ggttattttt actaccagca ctatcaagaa acccaagcag
    16141 tagaagctgg agaaaagaca gttgagcaat ttgtccaagc tttgaacaag ggagattata
    16201 acaaagctgc agaaatgaca tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag
    16261 agatcttaga taaataccag aatatatacg gtgctgctga tgtcaaagga cttcagatat
    16321 cgaatctaaa agtagataaa aaagacgatt ctacttacag tttttcatat aaagcaaaga
    16381 tgaatacctc attaggtgaa ttgaaagatc tttcttataa aggaacatta gacagaaatg
    16441 atggtcaaac cacgatcaac tggcaaccca acttggtttt tccagaaatg gaaggaaatg
    16501 acaaagtaag cctgaccaca caagaagcag caagagggaa tattatagat cgaaatggtg
    16561 aaccattagc aacaacaggc aaactaaaac aattaggagt cgttccaagt aaacttggag
    16621 atggaggcga aaaaacagcc aatatcaaag cgattgcttc ctccttcgac ttaacagaag
    16681 atgctatcaa tcaggcgatt tcacaaagct gggtacaacc cgattacttt gtcccattga
    16741 aaatcattga tggagcaacg ccagaacttc cagctggagc taccattcaa gaagtagacg
    16801 gcagatatta tcctttgggt gaagcagctg ctcagctgat tggttacgtg ggagatatca
    16861 cagcagaaga tattgataag aatccagaat taagcagtaa tggaaaaatc ggtcgctcag
    16921 gtttggagat ggcttttgat aaggatcttc gtgggacaac aggtgggaaa ttaagcatta
    16981 cagatgcaga cggtgtagag aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag
    17041 atatcaaatt gacaatcgat gcgaaggcac aaaaaacagc ttttgacagt ctaggaggaa
    17101 aagctggatc aactgttgcg acaacgccaa aaaccggtga tcttcttgcg cttgctagct
    17161 ctccaagcta tgatccaaac aaaatgacaa acgggatctc acaagaagat tacaaagctt
    17221 atgaagaaaa tcctgaacat ccattcatca gccgatttgc gacaggttat gctcctggat
    17281 ctacgtttaa aatgattaca gcagcaatcg gtctcgacaa cggcactatc gatccaaatg
    17341 aagtgttgac gatcaacggg cttaaatggc aaaaagacag ttcttgggga tcttatcaag
    17401 caacgcgtgt cagtagtgat gtatcacaag tagacttaaa aactgctttg atctattccg
    17461 ataatatata tgcggcacaa gaaacgttga aaatgggtga gaaaaaattt cgtacaggct
    17521 tagataaatt catttttggt gaagaccttg atttgccaat tagtatgaat ccagcacaaa
    17581 tttctaatga agatagcttt aactcagata tcttgctagc tgatactgga tatggacagg
    17641 gcgaacttct aattactcct atccagcaag cagcaatgta ttctgttttt accaacaatg
    17701 gcacacttgt ctatcctaaa ttgattgcag ataaagagac aaaagataag aagaatgtca
    17761 tcagcgaaac agcagtacaa acgatcgtgc cagatctgag agaagttgtg caagatgtaa
    17821 atggtacagc acattctctt tctgctttag ggattccgtt ggcagcgaaa actggtacag
    17881 cggaaatcaa agaaaaacag gatgtaaagg ggcaagagaa cagtttcttg tttgctttca
    17941 atcttgataa ccaaggttat atgatggtca gcatgttgga aaataaagaa gatgatgatt
    18001 cagcaaccaa acgagcatcg gaactattgc aatacctcaa ccaaaattat caataaaaaa
    18061 ttgcttttta agtacagtaa aaggactgtg gcgttatcgt cacagtcctt ttttcaaata
    18121 aacggttttc acgatgtctc tttcacaact tctttttgat ttactcacac ttcataaact
    18181 ttttttgtaa aatatttgag aacaagtgga gaaataagtg tacttaataa aatcacgata
    18241 actaaaggtg aatagtactg tggttcgatc aattgacttt gctgtccaat ttgtaaaatg
    18301 atcaaagcca tttctccacg cgaaatcata ccagcaccga ccatcaatga actatgttga
    18361 gaaaaaccgg caatctgagc accaaaataa cctccaagca atttggtcaa aatcgctaca
    18421 agcgtcaata tagaaataaa gagtagttgt tcactaaagt cagaaaaatc aacttctaat
    18481 cctacactga caaaaaatac tggtataaat actgcatatc ctaaagcctc tacattgtgg
    18541 tatacttctt ctttaacctt tgtctgactg acagctatcc cagcaaaaaa agcaccaatg
    18601 acagagctaa gcccaacaag atccgctacg taagacattc ctaaacaaat aactagcgat
    18661 acgatgataa tagaagaatt tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca
    18721 atccatttga ctaataagaa aataaaaaca aaataaatta gctgttcaat caaaacaagc
    18781 ggtaaagaag cattgctaga agcttccccc gttagaaaag ataggctgaa acttagaact
    18841 aaaacgacta aaatgtcatc taccacagaa gcacccaaaa ttgttgatcc ttcttttgta
    18901 ttcactacgt ttagttcttt taatacttct actgaaatac tgacagaagt tgccgcaaga
    18961 ataatcccaa agaaaatcgc ttcatttccc gaaacttgga atgctttgcc tgtcaaccag
    19021 ccaaaaacaa ccgggaagat gatcccaagc aaagcaacga acataccagg tcggaaatat
    19081 tttttcaaaa gagataaatc actttccaga ccagctaaaa acattaataa gatcacacca
    19141 atctccgaaa agtcatggac tagaatatca ggtttgaccc aattcaatcc agcttggcct
    19201 agcaaaacac caacaagtaa ttgtccaatc actgctggga tacctaatct ccgcgagata
    19261 tgtgagccaa tcgttgttga aatcaatatc aggcataaaa taccaataaa ttccattctc
    19321 atcattcctt tgtataaaat atagcccgaa cttagcataa catacattat tgaaaaaata
    19381 ggaaaatata ctcacaaaaa aaccaacaat ccaaaagatt tttttggatt gttggttttt
    19441 atacggtcta ttttaataac taaaaatgat taacgtatag tcatttttct tattctgatg
    19501 aaattgataa tctcctggat gcccatgaaa atcaaaataa agccgaatat ttggaataga
    19561 agcatcaaac ttttgaatgg attgaacaat aagacgaatc cgccaatcaa caacagaata
    19621 ctactataaa cactccaact tttagctttt gtattcatgc tgaagaacaa ctggaacaag
    19681 ctgttgatga taacagctaa tccaagaaga atcggcagga tagaaacgat tgcaggtgca
    19741 aaagcataga cgatcagagc taagatcagg aaaaaaagac cactgaacag accgatactc
    19801 caagaacctg ttttcctttt aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa
    19861 acaagatagg cggtgatcag ataaccgata aaatgaaaaa cagcagaagg gtttagcaca
    19921 atcgcaatcc cagaaataat ataaacagca gctcttaata aggcgtattt ttgaatattt
    19981 tgtttaatcg tctcaaacat ataagaaaac ctcctttgac ttattttaac gtgaactagt
    20041 cattttacca aattaaatgt tacaaaatga gcgaaggaga agaatatctc tggaaaaatc
    20101 aacttctgag gatcttttcc atttttttgc cttttgctag ctcatcgatc attttatcta
    20161 aataacgaat ttctttcatc aagttatcct cgatgtcttc tactcgtaca ccacaaacaa
    20221 cacctgtaat caaggaacgt gaaggattgg ggacaggggc ttccgcaaaa aattgttcga
    20281 aagtcacttg tttttgtagt tgatattcta gttctgtttg ggaataaccc gtcagccaac
    20341 aaatgatttc atctacttcc tcttttatac gatcttttct ctccgctttt gctacataca
    20401 taggataaac gcttgcaaaa cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc
    20461 tttctttttt tcaaattatt tactacttat agtatataag aaaaagagaa gaaaaagcga
    20521 ttattcaaaa aaacaaaaag gattgtgaca aaattcttgt caccatcctt ttttccgctc
    20581 tctgactttt tacaatcttc atagctcaag tgcgcatcag ataatcgaat gctcctaaag
    20641 ctgcagtagc tcctgaaccc attgaaacaa tgatttgttt gtatggacta tctgtacaat
    20701 caccagctgc gaaaacgccg gggatattcg ttgcgccatt tttctcggtc actatttcat
    20761 ttcttggatt catttcgatc gttccatcta gccaatccgt attagggagt aagccgacta
    20821 gaatgaatac tcctgaaaca gctagtgtat gcatctccat cgtacggcga tcgatatagg
    20881 agagagattc tacctgattc gtaccgctga tttctttcgt cgctgcatgt ttgataacag
    20941 tgacattcgc aagagagact agtttatcct gtaagatttg atctgctttt aattctggta
    21001 agaattctag aacatataca tgttttgcta acccagccaa atcgattgct gcttcaacac
    21061 cagaattacc accaccgata acagcaatct cttttccttt aaataaaggg ccgtcacagt
    21121 gtgggcaata agcaattcct ttatttttaa actctttttc gcctggtaca ttgatagaac
    21181 gccagtgcgc acctgtcgcg atgatggcgg tttttgcttt aagtgatata ccgttagcta
    21241 agccaatatt aataaaatgt tgctctttat ttaaggaaac ggcctgttga ttcatcataa
    21301 tgtcgatagg gtaactacgc acatgacttt ctacttgctg catcaatttt ggaccttctg
    21361 tgtaagaggt accaatcata ttttcaatcc ctagtgtttc aacgacttgt ccgccgaaag
    21421 aatcagcgac aagtcctgta cggattcctt tacgtgctgc atagatagct gcactactag
    21481 ctgctggtcc accgccaatc actaatacat cataaggttc tttttcaaac aactcttctt
    21541 caatcaaagg gccagtgatt ttttcaacaa tttgttcgat cgtcatgcgt ccgctactaa
    21601 attcttctcc atttaaaaac acagttggca ctgccatgat ttttttatca tcgatttctt
    21661 tttggaacat cccaccttca atcatcgtat gagtaatatt tggatttaat acagctaata
    21721 tattcaatgc ctgtacaacg tcggggcaat tatgacaagt caagctaaca taactgtcaa
    21781 aatgcaatgt ttgtgttatt tttttgattt taagactcaa agattcactg attttcggtg
    21841 cccgtccact aatttgtaac atagccagta aaaaagagga aaattcatga ccgaggggga
    21901 taccagcaaa aacgattcca ctttctttgc caactggatt gatagaaaaa ctaggtgtgc
    21961 gcatcgaatc tcttttttcc caagagattt taggagacat ttctgctatt tccttcgtaa
    22021 attcagctag tttttgacta ttttcactat tatccaaact aacagaaaaa acaataggag
    22081 attcaatcag tgctaaatat tgttcgagat tttgtttagt ttctttgtct aacatatcca
    22141 ccacctaaat ctttccaaca agatcaaaac ttggtttcaa tgtttcgcca ctttctttcc
    22201 attttgccgg acaaacttca cctgggtggg tacgtacata ttgagcagcg cgaatcttat
    22261 caatcagtgc actagcgtca cgaccgattc catctgcatt gatttccata gcttgaacga
    22321 ttccgtctgg atcaataata aatgttcctc gctgagctaa tccttgctct tcatccaaaa
    22381 catcaaaaat acgagaaatc tgatgagacg gatcaccgat catcacgtat tcgattttac
    22441 cgatagcatc tgatgtatca tgccatgctt tgtgagtaaa gtgggtatcg gtagagcaag
    22501 aaaagacttc aacacctaaa gatttcaatg tactgtattg ttcttgtaag tcttctaatt
    22561 cagtaggaca aacaaaagta aaatcagctg gatagaagca aagaatactc cagttcccca
    22621 taagatcttt ttcactgact tctaaaaatt ctccttgatg ataagcatgt gcattaaatt
    22681 cttcgatttt tttcccaatc aatgacatag taaaacctcc aataataatt atttatttat
    22741 aattattgta atttagttgg agttgcaaag tcaatggata ttttgttatt taataaagat
    22801 tactcaaaaa cgaaggttaa ttataaaaat agaagaggtc gcaagaaaat gctactgctg
    22861 gaaatactcg ggataacgtt gagtaacagt ttcctgttct ttcagcatca tgtgtaagtg
    22921 caatcccgtt tgaaatcgag cttcttctgg ttttttatca actaaagcct tgagtaaatc
    22981 ataatgttgt tccatgatcg tttcccatgg aagcccctga atggtcactc gtaaccagcg
    23041 gaaacgttct aaatgggtat tatggtcatc taaccagttc caaacttctt ttcttcctgc
    23101 aatttcgaaa caagcttcat gaaataaatt atctgcacgg aaaaaagcgc gttcgtcatt
    23161 tattttgact gctttttctt gttcttctaa aatcgtttct agcactattc gtgtttgtgt
    23221 gtccaatttg gaacaacatt caatcatgat ttgctcttcc aactgttctc ggacaaagcg
    23281 agcattttct gcagaaacaa gattaatttt ggaaacatac gtaccacttt gaggtatcgt
    23341 atacacgagt tcttgctgac gtagttgaat caatgcttcg cgaatgggag tccggccgat
    23401 agcaagatgt tcctccagtg ccttttcaga tattttcctt ccaggttcta agtcagcata
    23461 gataatttgt ttacgaatcg cttgataagc ttgtgattgc aagctgcttt tcatctttat
    23521 tcctccaaaa tcatttctat gaacgtagta taacagaaga ggtctgaaaa acaatctggt
    23581 atgtcacatt aactagtaaa tttgtataaa tagtaaaatt ttggttgaca tagcgatttt
    23641 tattgcataa aattcacttg aatacaaaaa ctaatatgtt agtttaggag gggaaaaatg
    23701 attgatttaa acaactggag gaactttatc cctgaattat ctcaaaaaga agtagaaata
    23761 cctcattttg accgtgcatc attaagagaa aatggacaaa aaaatcctgt ctggatccat
    23821 ttcggtggcg gaaatcttta tcgaggattt catgcagaaa tcgctcaacg tttagcggat
    23881 ctaggcgaat tgtccactgg tgtcattgtt tgtgaaacat tcgatgaaga aataattgaa
    23941 agggcttacc aagattttga tgatttgatt cttgaagtgg tcatgccaag cagtggagag
    24001 ttcgtcaaac gtatattagc tgcgacagct gacagtctgt attgtcatcc agattgttct
    24061 gaacactttg ataagatgat tcgacacttt gaaaatccta gtttacaatt agttactttt
    24121 gctattactg aaaaaggtta tcgattgaaa gatacaaata ataaatatat aggaccgatt
    24181 gcagtcgata taaaaaatgg tccaaagaag ccatcacata caatgagtat tcttgctgtg
    24241 ctactttata aacgtttttt atctggagct tggccgattg cacttgtctc tacagataat
    24301 ttttcacaaa acggtagtca attacgtact tctttgctaa cgatcgcaaa agaatggcaa
    24361 aaggaaggtt atgtacaaaa tacatttatc caatacttat cgaatgaaga aattgttgcg
    24421 tttccatgga cgatgattga tcgtatcacc ccgaatcctt ccttgtcagt tagcaaaatg
    24481 ttggcaaaag agggcttttc aaatatgtcg attttccaaa ccgcaaaagg aaataatatt
    24541 gctccatttg tcaatacaga ggacatccat tatttaatca tggaagatca ttttcccaat
    24601 ggacgtccga aattcgaaaa agctggggta ttcttgactg atcgtgcaac ggtagataaa
    24661 gcagatacga tgaaggtgac gacttgcctt aatcctttgc atacgtcttt agctattatg
    24721 ggttgtttac tggggtatag gagcatagct gaggagatga aagatccaga tttatcgctg
    24781 ctagtttctg aaatcgggta caatgaaggc ctcccagttg tggagtctcc aggtatcatt
    24841 gatccaaaga tctttctgga agaagtaatt actgtgcgct tttctaatcc aaatattcct
    24901 aatatgcctc agcgaatcgc cacggatacc tctcaaaagg tggcgattcg ttatggtgag
    24961 acgattaaaa aatatatgga aatagaagtt aaagacccgc agacacttgt attcatacca
    25021 ttgactctcg ctgcttggct tcgatacctt attggcattg atgatcaagg aaaaccattc
    25081 atcccgagtc cagacccact attgacagac cttcagcata agctttccga tattcaactg
    25141 ggtacgcaag atacatcaca tattcatgag aaaatggctc ctattttgtc aaataaagaa
    25201 atttttggtg tagatcttta ccaagcagag cttggcagaa aaatagaaga aatgttcatc
    25261 aagatggtag caggaccaaa tgccgtacga aaaacattac aggattactt acaagaacat
    25321 ggaggaaatt tatcatggaa atgactttta gatggtacgg atcacaggaa gaaaagatta
    25381 ccttagatga aatcagacag attcctgcgg tcaaagggat tgtaggcaca ttgatggata
    25441 taccagtagg agaggtatgg cctagggaga ggatcaaaga actaaaagaa gaaattgagt
    25501 ctcgtgggtt aacactaaaa gtaattgaaa gcgtcaatgt gcatgatgcc attaaaattg
    25561 gattaccttc ccgtgagcag tatatcgaaa actataagca gacaatccga aacttggcag
    25621 aatatggaat agaagtcatc tgctataatt ttatgcctgt atttgactgg gtgaaatctg
    25681 accttgacta ccgcttagaa gacggctcat ccacgttagc ttttatatct gatgatattc
    25741 cagatgatcc aagagaaatc gtcgcgcgca tcgaacattc cagcaatgaa tttggattgc
    25801 cgggatggga acctgagcgc ttagctccta tcaaatcctt gcttgaagcc tatgcatcag
    25861 tagacgaaga aaaacttcga gaaaattttg cctattttct ccaatcaatc atcccaacat
    25921 gtgaagaggt aggggtcaaa atggctgtac atccagatga tccgccatac tcgatttttg
    25981 gattagctcg aatcgtaaag aatcgagaag atttagattg gttgtgtcgc atagtagaca
    26041 gcccttataa cggaatcact ctttgtacag ggagtattgc agaagatcca gaaaatgatg
    26101 tatatgggat tttagcagaa ttttgccaaa gagatcgaat cccatttgca catgttcgaa
    26161 atattaaatt tttagctaat cgagatttct atgaggcacc acataaatcc gaatacggtt
    26221 cattagacat gtaccgcata ctaaaaacaa tgtatgacaa cggatttaac ggatacatcc
    26281 gtcctgatca tggaagaatg atctggaatg agacaggaag accaggatac ggcttatatg
    26341 atcgagcatt aggtgctgtt tatctaaatg gattatggga agcaatcgaa aaaggaaaca
    26401 aaagtaaaga taattagtga aaaaaactgt aattttatta tgtaaataag aaggatgttc
    26461 aaattcttga tacataaatc cggaggttgt taataagcat tcggatttat gtatcttatt
    26521 tggtctagga agtaacttga ttttcttttt tcgattgatc catgagtgct tcttctttta
    26581 attcaaagat atattctgag atcctcataa tcactttttt ccagtcttct ggtgtgatga
    26641 ttccttttaa aatatagatt tttttgatat gagaaggcaa ttgtttcaaa gggcagtcgc
    26701 tgataacgat gttgcagttc tccataaaat cattttgtat tttgatcgat tcatttgaaa
    26761 aaatttgttc tatcttaacg atcagatctt tactgatata aaaatcacgc gtatagttca
    26821 caccaatcac aattcgcggc tgttttttgt agcgatcata caaatgtact aggtcttcaa
    26881 ccatccattg cacattttcc ttttctaaaa taactggtaa caagtgttga ttttgttctc
    26941 tgaaatccat ataaaattgt tcaatggcgg tttggacttc tttcaatacg ctatgttctt
    27001 gataaagcat gaaagtagga aggggcgtat ttggttgatt gaagttaaga aatccattat
    27061 aaagtttatt tcgtgcgaat gccaatgagt aaagaatctt ttctttttca ggtataaaca
    27121 aatcaaatac agtacaaagg gactccacca tttctcttga ataaagaaca tgaggaacgt
    27181 ttgctttttt taaaagtttg tattgctcca agcttgtttc ttccgattcg attttcgtga
    27241 tagaaagtcg agctaaaata ttcactaaga tgcgttgttc gcgacaaatt ggcagattag
    27301 gtcgcaaaat atctacagag agatcaataa aaaagtcaat atattcatac tctgtgaatt
    27361 ctttttgtac gtctttcaca gaagttgcag gaaaattctc tttacatatt tttaatagaa
    27421 aatataattt atctatcata ccgctattta gtcggtcaag tacttctttt ttaaaaaaag
    27481 tattcatcat aaaagccact acttgcggat cttcatcata aaattctgta gcagtacttg
    27541 aaaagatgtt ccaataaata tctaatgctg catattgaat ttgtacctct tttccagaaa
    27601 aagactttct gccattatta gaaaaggaga tagacaaacc gtactatgct aaaaatgcat
    27661 taatcttttt cattttcgta tacaaataag aggcactata gtttgtttgc tgactgaaac
    27721 tgataatcga atttgtctga cagctgaaca aagaagtcaa gatctgataa ctgactgatt
    27781 cgttgcagta tcttccaaat agacgataat agcagtaagc cggatcaatt ccttcaaaat
    27841 tcaactttaa aaaaggtttt tgcttatcaa tatgcagttc tgttagattg agacgctcaa
    27901 agtcttgttc taattctttg atgtatagta aaaaatttga ttcagacata tggaaatgat
    27961 ctgctaaata ttgtgtagta ataaagaaac gatgttcaga taggaaaaga aaaatatcta
    28021 attttgtatt gctttttttc ttcaagataa acgttttaat cgcttcttga gtttgcatga
    28081 aagaggctcc tttttactta aattgttaaa acttgaaaat attactgttt ttttcataaa
    28141 tttagacaaa aaaacagtaa acatataggt agaatctcta ctatcttatt tcttgcatac
    28201 aaaaaaatca aggagtgaga gagttatgag atattcaacc atcttaatat caacatttgc
    28261 agccttaagc ttatcgctta ctataaatca gacagtccac acagaggaga cagaaacagc
    28321 caaatggaca gaaaccaagt gggccaacgt caatcgaatt acctttcagg atgaattttc
    28381 atccttcaat tcaggagtcg caaaattaag aaaaggacaa acagcgacca tcaaagcaaa
    28441 aattgactat tcgggagact tagcacctat actacaatca gctgttacat ttgagaatgt
    28501 tgatcccact ttgtcgattg gctatgacga gaacactttg actatccaac acaagcaagc
    28561 gatatttacc ttcaccgtta cattaaatca ggacttgtta aaaccagcgc tttttactgt
    28621 caaggtatca gatggactac cgaacgatca tcatttaacg ccgtatagtc agcggcagac
    28681 aattgagcaa gattcagcca tcgactcagg aggagatctt gttgaagaac caacagataa
    28741 accagaaaat gaaaataagc cagaagtacc gcctacagaa aatccagacg gagaacagaa
    28801 accggaaata gaacctggag aagaacccga tacagaaact caaccagagc cagacaataa
    28861 atcaaaacct gaaataaccc ctggagaaaa accagatgta actccagaac cagatacgga
    28921 ttctggaaac caaacagtac cggaaacaaa tccagacaca gataatgaaa ctgaaaatcc
    28981 agaaaaacca gaggtagatc cagaagaaaa accagatgtg actccagaac cagatacgga
    29041 cgcaagagac caaggaatac cagaaaaaat aaacaaaaaa acaattcaag aggatggaaa
    29101 aaaagagagt aaaaaatcaa atctagcaat tctaaaaata aatgaagagc agctgaataa
    29161 aaaatcacga atatttgata gcgcccaatc agctgaaacc ttaaaaagta gcaaggatac
    29221 aacctttgct gtcctgaaac gaaaaataaa cagctaccaa aatccggaga gtcacaaaac
    29281 aaagttatac tgtggagcgg aatcattcta ttaagtatag caacaatgct ttctgcaaaa
    29341 agatttaaac aaaacagatc tctctaacat ttgaataatg tctgtgaacg catttttcaa
    29401 ataaaaagaa atatccagaa aatgacccca aaataccaga ttgaaatctg atatttgggg
    29461 tcgttcatac tttatctttc ttcatattta ttttaaggga tatctgtttc ttttggaaga
    29521 tggttcggga tctttttcac tttcaataca tctaaaaaga aggtaaagat cggaatgccg
    29581 acaatcaagc cccaaacgcc aaagaaccgt tcgctaacaa gtaagaccac aaatgtataa
    29641 aaaatcggca gttctgtttt gctggacatc aacttcggat tcagcacata agactcaatt
    29701 agatggacaa tcaaaatcgt aatcaggata taaacaacat ctcggatgcc tccttgggaa
    29761 taagctatga aagatagagg aatacaagaa atgataacac ctgctactgg gatcagactt
    29821 aaaatgaaaa tcatgatcgc taaactcagc agctgatgaa agccaaatac agccaaggcg
    29881 attgtagtga gcaacgtatt taaaagagcg acaacaaatt gagcttctaa taccaaacca
    29941 aatgtattga caaacttatc ggcaaaatag taaatatctt gaaagaacca agaaaattca
    30001 ctttttaaaa acaatcttga gaatacaatt gtttttttct tttcaatcat aaaaaagaaa
    30061 cttaagatga atgacatagc aaacgccact gccatttttc ctatatcctg taaatagcca
    30121 agcgcaatcg aagcgccatt ttgaagctgt tcaaaaaaat cgtttcgctc aatataagta
    30181 tggatatatt gtaaaacagg atctgcatct tttggctgat gctcgtaaaa attaataacg
    30241 gactggatca tgtcaaatgt ttgatgaata agcatcggga tatactttgt gaatgccaga
    30301 tagataaggt aaacgatcag tgtataaatc atgatagcta aaacacctgt agggattcgg
    30361 acaaatcgct gaatgatttg aacaagatga atcatcaaat aagtaaagat aaatgtgagc
    30421 aaaatggttg taatcatact tctaactaag aaaagaacaa aaacgacaaa taataaaaca
    30481 gtgagacggc gtattttttc attcgccaaa aaacgctgat aaacggtcaa atcgacaacc
    30541 tccaaataac aaaaaataaa cacgttattc attatatgat ggaatatgaa aataagaaag
    30601 agcaaaaaga aaaaccgcta aatgacgtta tcttatgagt tggttttcct cataaaatcg
    30661 ttcatctagc agtttcagcc ctttttctgg tcttgatgaa aattatctag catttttcgg
    30721 gtcagatgtt gcagtgtatc caattcttcc tgttcaagtg gaagataaga cttgatcttt
    30781 tgtggaatac cttctgcttg tttttgcaac gcagcacctt gagcagtcag cgtaacattt
    30841 acgattcttt catcacccgt ttcacgctga cgattaatat aatttttttc tgctaacttt
    30901 tttagtaagg gagtcagcgt accagaatcc agacacaaat catttcctag ctctttcact
    30961 gaagtctttt tcttttccca taaaactaac atcacaagat actgggtata ggtaagattc
    31021 agcggcttga ggaggggagt gtagtgtcta atgatttctt tagaggcggc atataaagga
    31081 aaacatactt gattaactaa atatagatct tccatttttc cacctccgct gttttactat
    31141 agtgtatcat agcggattta cacaaaaaag tctattcact aactgctttc agagtaactt
    31201 ctatatttcc agctgtagct tttgaatacg gacacacttt atgcgctgcc tctaataatt
    31261 cttgcgtttc ttccagagaa agaccttcga tagatccttc aatctctaca cctaagacaa
    31321 agctcgtttc actttctgaa taaagggaaa cttgtgcact gacagtagaa gccccagaga
    31381 tatttttttg ctgtttgatc aattctaatg cactgttaaa acatgcactg aaaccagctg
    31441 caaacaattg ttcagggttg gttgcatttt cgacgcgttt accaggagct gcaatattca
    31501 attcgaatgt acgatcggga ttatgcactt ctccagagcg tccgcctgtg ttgatcattt
    31561 tcgtagaata catttttttc ataaaattac cacctttaat tatattttat acaattgaat
    31621 tttacacaat ataattgtgg aaagcaaaga aatggattgg cataagataa ataagagaaa
    31681 gcacatgaaa cagacgcgct ttctcgaata gcaaacatat acaaaaaata tcagagacaa
    31741 aaaataatct catcataaac attacatata tttctcaaaa agttgttttc tttcttcaaa
    31801 aattacttca cgatggacct gatgaagcat ccataaataa ccaaaaggat ctctgaacat
    31861 tgagttagtc acaccaaagt ccatcatttc tgtcatttct tgaatctcta cacagccatt
    31921 tcgtaatgct ttattatgtg tgtcctcgat atcagagacg atgatattga accagatcgt
    31981 atttggatgt tcagcagtag gagcagtcaa accaagagac gtgttttcat ccagcatatg
    32041 gaatctcgta ccatacaagg taaaaatgac ttcattattg cctctagggt aatctgttgc
    32101 ctcaatcagt tgtatttcat caaagattgt ttgatacagt tctagtgctt tcaaactatc
    32161 atttaataca aaatcgattt ccgttccttt atgcataaag atctcctctc cttttcagtc
    32221 gaacagttac tagattcttt tattatacca gctaaagaaa acgcattcaa aaaaatagcg
    32281 gatttactta ccaactcatt cgattggatg ataagtaaat ccgcgtccca ttacttctgc
    32341 tacgctgctg acaatgacaa aggcacttgg atcgatttct tctatcaatg gtttcattcg
    32401 ggaaaattca tgatcagtca caacgatcaa taaaacctct tttgactttc gattataccc
    32461 gccttttgcc tctagcaggg taaggccacg tccaatctca gcacttaagc gaaggcggat
    32521 gtcttccaaa taatgttcac tcatgaccat aacagatttc tttcgattga tacctgtttc
    32581 gatataagtc atgacaattg aagtgattgc aatagataaa atagcataaa gaaactcttc
    32641 tattccaaaa ataaacagat taagagaaac aacgaatgcg tctgtaaaaa ataagccaat
    32701 cgaagtattc aagtggaaat attttttaaa aatgagggga gggatcgtcg taccgccgct
    32761 agaagagttg tttttatata aaatcgcaac tcctaaagca aaaatcgcac ttccaaaaat
    32821 gacagacaac aaccgatctg aagtaatcat tgtttctgga actattgcaa tgattaaagg
    32881 aaaaaccaag cttccgaaaa gtactttaaa aaagggtttt ttacctaaaa atagaagagc
    32941 tagtaccaac ataacgatat tcaacactaa aataactata gctcggtcaa agcctaaagc
    33001 agcttccagt aatattccta gaccgctgac acctccagca gcaatatgat gaggccctaa
    33061 aaacatattg atagcgactc ctaatatgaa gagggcaaaa ataatcttaa tgtatgtcca
    33121 tataaattgt ctcgtttctt tcatttattc acttccttaa ttcattaata atactcttat
    33181 cttattataa cttacttttc accttaagtt aactttaatt aataaaaaaa ttaggctagt
    33241 ctaaattatt atgtttcatt cttctagaat atacttctac gccaaaaact gttaaaaaac
    33301 ttttacttta gaaaattgga gaaattgatc gcttaagata aattcaggac actaaatagt
    33361 acaggaagta gactaaaact tttaaaagtt ttaagtctat ctatttggca agtttaaaaa
    33421 ataaaaattc ggtatcaaaa aactgttacc gataacattt ttgaaagcgt tgtcttttgc
    33481 ttctctatca cttatagtga tgacgtcata ttaaaacaca aggaggaaat caatggatac
    33541 agctgcaatt tatcatcgac cggaaagtga atatgcattt ctatatacga atacaagatt
    33601 tcgtattcgg cttcgtacta ggaaaaatga tatcaaaaaa gtatatgtgc tatgtggaga
    33661 tccatatacc ataacaactg aaaaatggta tcaaaaacaa cgaccaatga aaaaatgtct
    33721 aagtaccaat gttcatgatt attgggaaat tgaagtgacg agcgaaacaa gtcgcttaca
    33781 gtatgcgttc catgttgttg gggaagataa cacggactgt ttttacggtg atcaaggaat
    33841 ttttccttat cgagaaaatg tgattactga accaaatttt tattttcgaa taccttattt
    33901 tcatcaaatc gatcgattca ctattccaga atgggtaaaa gaaacagtat ggtatcaaat
    33961 ttttcccgaa agatttgcaa acggggacaa agcaaatgac cccgaacaca ctttaccttg
    34021 gggaagtaaa gatccagacc gagaagactt tttcggggga gatttgcaag gtgtgatgga
    34081 tcatttggat tacttgacgg accttggcat caatggcatc tatttttgtc ccatatttaa
    34141 agccacatcc aatcataaat acgatacgat tgattattat gaggtggatc ccgcttttgg
    34201 cgataaaaaa cttttaaaaa acttgattga tgaagcacat aaaagaggga tacgtatcat
    34261 gttcgatgct gtattcaatc atatgggtgt ccattcacca cagtggcaag acgttctcaa
    34321 aaacggagaa aaatcgatct ataaagactg gttccacatc cgttttttcc cagtagattc
    34381 ttatcagatg actgacttac cagaaactgc tgaaaatatc ccatatgata cttttgcttt
    34441 tactccattc atgccgaaac taaatacagc aaatccagaa gtgcaaaaat atttgctgga
    34501 tatcgcaact tattggataa aagaattcga tattgatgga tggcgtcttg atgttgctaa
    34561 tgaagtcgat catcattttt ggaaaaaatt cagagaagca gttacagaaa tcaaaccaga
    34621 catctatatc cttggagaga tttggcattc ctctcaagct tggctgcaag gggatgaatt
    34681 ccatgcagtg atgaattatg catttacaga ttcgattaaa gattattttg caaaaaagaa
    34741 aatcacagct tcacaaatgg tcagtggaat gaatcatcag caaatgcttt acagagatca
    34801 ggtgaacgaa ggaacattca acttattaga ttctcatgat actgccagga tcttgaccct
    34861 ttgccaaggt aacaaagaat tgatgaagtc tgtgatggcc tttatgtttc ttcaaaaagg
    34921 gtctccttgt atctattacg ggacagaaat agggatgact ggtgaagatg acccggattg
    34981 ccgcaaatgt atgatatggg agaaggagga acaagatcta gagctattcg gttttataaa
    35041 agaacttgtt tctctaagga aacagttgtc taagattatt tctgaaggtt ctactcagtg
    35101 gctaatagta aatgatcgtg aagataagct ctatttcaca cggaaactag agggacagat
    35161 catctatgtt tactttaatc agtcaaaaga accttgggta gtagaacagg aaaatgaggt
    35221 gattcttagt caaaattgtc agttgttaga agacgggaaa gcagagatac gacaatacgg
    35281 atttttgatc atgtgttgaa aactgttacc ggtaacaaca agaaagcgtt tgcaaatgaa
    35341 gatttgtgcc cattataata agggtgtaac agcacgttga atacaaagga ggaaatgaag
    35401 atgaaaaaaa gaagtgtgaa acagttgggt ttagggttaa tctctttagg aatcttaggc
    35461 ggattagcgg cttgtggcaa tggtaactct tcagcagaag gggaagccga tgaaggaaaa
    35521 acgttaacag tttccgttga ttctggatat aaagattata taaacgaaat aaaaaacact
    35581 ttcgaaaaag aaaacgatgt gaaaatcaag ctagtagaaa aggacatgtt cgatcagttg
    35641 gaatcattag cgttagatgg gcctgctgga aagggacctg acgttatgat ggcagcatat
    35701 gaccggatcg gtgcgttagg acagcaagga catctggcgg aagtaaaact gggaaatgaa
    35761 gcagcttacg atgaaacaga caaagcccaa gtgacttatg atggaaaaat ctacggagaa
    35821 cctgcagtaa tcgagaccct tgttttgtac tataacaaag atttagtcga tacagcacca
    35881 tcaacgttta aagatttgga gaatctatcc aaagacagtc gttttgcttt tgagtcagaa
    35941 gcaggaaaaa atacaggatt tttagctaaa tggactgact tttattattc ctatggttta
    36001 attgcaggtt acggtggcta tgtatttggt gatgatggca caaatccttc agatatcggt
    36061 ttgaataatg caggtgctgt agaaggaatc tcttatgcaa cagattggtt caaaaatgtt
    36121 tggccaaagg gaatgcaaga cataaaaagt gcaggagact tcgccagcca gcaattcatg
    36181 tccaataaaa ctgcagcaat cattgacggt ccatggcaag cgcaaacata caaagaaaac
    36241 aatatcaatt atggtgtagc caaaattcct actctaaata atggacagcc ttatcaaccg
    36301 tttggcggag gaaaaggatg ggtagtcagc aattatgcta aaaacaaaga cctctctcaa
    36361 aaatggcttg attatgtaac gaatcaggaa aatcaagaaa aattctttga aatggtaaat
    36421 gaaatccctg caaaccaaca agctagggaa acagcaaagg ctaaaaacga tgaattaaca
    36481 acagctgtga ttgaacaata tgaaacagct caagcgatgc caaacatccc agaaatgggc
    36541 gaagtttgga caggtgcaga aaacttgatg ttcgatgcag catcaggttc aaaaacacca
    36601 aaagaatccg cagatgaagc agtaaaaaca atcagtgaag ccatagaaca aaaatactga
    36661 ttaaaatgat aaccgtccat ttttataaaa aatggaacag gtgcttcaga taaaagtggc
    36721 aagagcgaga tggaaaatta tcttgctctt gttgccatta tcttaaaagc agtttgcggt
    36781 ttagcaagtt tagagggggc attcagatgc agcaagataa aacaagcaat atcaacgaaa
    36841 aaaaaatcac gctttgttca cttattccag ggatggggca gtttgtaaat ggccaagctt
    36901 ttaaaggtat cgtttttttc gccatattcg tcctattcat tggtcagatg cttctgggcg
    36961 gatttcatgc attggtagga ttggttactt tgggaagtat accaatggaa gatcattcat
    37021 tatttatttt gattgaaggc actttgcaat tcatcattac aatcattttc ttgatttttt
    37081 acggtttgaa tcttttagac gcttatcgag tagcgaagat gcgtcaagag ggcaaaaaaa
    37141 tcaatcggac gatgaaggaa gtcatacata atataggtga tgaaggtttt ccttatctat
    37201 tgacacttcc cgcttatctt ctcatgattt ttacgatcat tttcccagtc ctagttacat
    37261 tgttcactgc atttactaat tatgacttca atcatattcc accagccagt ttaattgatt
    37321 gggtaggggc taaaaacttt tttagtatct tctttttaag ctcatatcgt aatacttttt
    37381 ttgcagtctt ttcgtggacc gtgatttgga ctttatgtgc aacaactttg caaatcaccc
    37441 ttggtattct cacagcggtt gttgctcacc agaaatttat taaattcaaa cgtattttcg
    37501 gtatcatttt cttattacca tgggcggtcc cagcttttat tactatcatg agtttttcga
    37561 atatattcaa cgatagcgta ggtgccatca atactcaagt gattcctttc ataaaccagc
    37621 ttccttttgt agatatcagt gcaatctctt ggaaaacgga cccgaactgg acgaaagtag
    37681 caattatctt agtgcagggc tggttaggat ttccctatat ctatgtgatg gttacaggaa
    37741 tcttacaatc gatttcagaa gacttatatg aagcagcaaa aatcgacgga gcaaatgcat
    37801 ggcagcgctt ttcaaacatc acgcttcctg ctgtcttcct agtggcagcg ccaacattta
    37861 ttacacagta tacggggaat ttcaataatt tctccatgat ctatctgttt aacaatggcg
    37921 gtcccggaag cgtaggcgga ggagctggtt ctactgatat tcttatttcc tggatctata
    37981 aacttacgac tggaacttct ccgcagtatt ctatggcttc agcaatcacg ttgattatct
    38041 ccgtcatcgt tatctgtgtg tcattgctgg tatttaaaaa gacaaatgcg ttcaatatgg
    38101 gggattaaca atgaaaaaat ttcattcgag tgctcgattc caaaaacgac tttctcactt
    38161 ttttacttac ttttttcttt taatactgtc agtcattatc atctatccgc ttttgatcac
    38221 agcaagtact gccttcaagt caggaaatat tgcagctttt agtttagatc tttcaagtga
    38281 ctggacttta ggaaacttta ctcggctatt cagtgaaaca ctttacggga cttggtataa
    38341 aaatacattg atcatttctg tcctaacaat gatcgtacaa gtgactgtag taactttggc
    38401 gggctatact tatagtcgct atcgttttat cggccgcaaa tcaagtttga tgttcttttt
    38461 agtgattcag atggtaccga ctatggcagc attgactgcc ttttatgtca tggctttctt
    38521 attagaagcg ctcgataagt actggttctt gacaatgatc tatataggcg gtggaattcc
    38581 aatgaatact tggctgatga aaggatactt tgacactgta ccaatagatt tagatgaatc
    38641 tgctaaactt gatggggcag ggcatttcag gatttttttc cagattgtat taccgcttgt
    38701 caaaccgatg ctcgccgttc aagcattatg ggcttttatg ggaccttttg gggatttcat
    38761 gttagctaag ttcctgcttc gaacacctga aaatcagact gttgctgtcg gcttacaaac
    38821 atttatttct gatgtgacaa accaaagagt ctctcttttt gctgctgggg caattttgat
    38881 tgcactaccg atttgtctat tattcttctt tttacaaaag aactttgtta ccggcctgac
    38941 atctggcgga acaaaaggat aggagcagag tatcatgcaa acatttccgt taaactattt
    39001 ttccgcatta cgtacgccaa ctcaagtgtt tgcgggacgc aaactattat catggcctaa
    39061 atttttcttg atatttgtat ttttagtttc cttgatggtc atgcctgtga ctctttttta
    39121 tgccaatcag atccaagcga ttccattaga acaatttcta tccgttcatt cgctgatcga
    39181 tgaacaagga acccaaaaat tttctgaact tgaattatct gaaactgggt tacaagcatc
    39241 gcaacaaacg atagctgtga cccctgaaat tttggtaggt gtgtctttat ctgagaaaca
    39301 acaatccgat catggaactt ttattgattt tgagaaggaa caatgggtga tccaacaaaa
    39361 ggataaatct ggtatacgcc gctatacgat gaattatagc ccatctttcc aaccagattc
    39421 tgtacgtacg cccgaagatt tccaacgatt tttagaaaga gaattttatg ccagtaatcg
    39481 tccaacaatc attttatctt atagtttaag tcttggtctt gtcctatttg tcatgacgag
    39541 tctcatttta ttcggggctt cctttttctt gtggatgaca agaaaaagcc aactttcatc
    39601 cattcataca ttcaaagaga gtgcaaattt gatgctgaat gttatgggaa taggttccat
    39661 ggcagcagca gttgtagggt ttatccactt tgactttatc ttgatgctaa gtgtccaaac
    39721 aacgataact gtcttgctgc ttttatgggt ttttgtcaaa acaagattca aagataagcg
    39781 tgtcgaatga caagtttaag cgatttatgc gacaatgaat gtgaaaaaaa tgaaaaataa
    39841 agtagttttt gaaaaatttc tgaaaaaatt aaacttctta gttgattctt ttctttttta
    39901 gtattaacgt aatagggtag tttttttaag gaggaaaatc atgttagaga aaactttata
    39961 caatcaatta ttttcacgtt ctttctcgct ccctgtcgaa gtaaattatt gggacggtac
    40021 aacgaaacaa tacggagata cggacactcc tacccaaatc aaaatcaata tccatgaaga
    40081 aatcccaatg aaagagatta caagtaacgc atctttggca ctaggagaag cctatatgga
    40141 tcatcgaatc gagatcgagg gaagtatcca agcattgatc aacgatgctt atcagcaagc
    40201 aaacagcttt ttacgaggaa aagattatct aaaatggctg ccaaagaaag aaaaacatac
    40261 gaaacaaaaa aacaaagaag atatccatgc gcattatgac ttaggtaacg acttttataa
    40321 actttggcta gatccaactt tgacttattc ttgtgcttat ttcaatacac ctgaagatac
    40381 gttggaacaa gcacaaatca ataaagtcca tcatatctta gacaaactct ttatcaaaga
    40441 aggcgaaacg ttgcttgata tcggttgtgg ctggggaaca ttgatgttca ctgctaccaa
    40501 agagtataat gtcaaggcaa ctgggatcac actaagtgaa gaacaatacg atttgattac
    40561 caaaagaata aaagaagaac atttagaaga taaatgccgt gtattattaa tggattaccg
    40621 cgaactaaaa ggcgaaacgt ttgatcatat cacaagtgta ggtatgttcg aacatgtagg
    40681 ttctgaaaat cttgaaggtt atttcaaagt ggtaaaagat ttattgaaac caaaaggaac
    40741 agctttgatc catggaatca gccgacaaca aggcggtgcg acaaatgctt ggatcaataa
    40801 atatattttc cctggcggtt atgtccctgg tgttactgaa ttagttgata atattacaaa
    40861 aaacgatttg caactgattg atttggaaag cttacgtcgt gactaccaat tgacattaga
    40921 acattggaca aagaattttc atcaagtcca agatcaagtc acgaaagaaa tgggtgattc
    40981 cttctatcga atgtgggatc tatatttaca agcttgtgca gcttcatttg aatcaagtaa
    41041 tatcgatgtg attcaatatt tgcttgttca acctggaaac aatgacatcc caatgcatcg
    41101 tgcataattg aggcaaaagt actgtttaaa gactatttaa aaagaagcta agttttatga
    41161 tgctccagaa gtgaactttt ttgtctaact tctaggattg tctctaaagc ttagtttctt
    41221 tttctttata ttttcataat aaaagtttgg ttctttaatt tttaaacatg acagaaaatt
    41281 ttttcttcgt tatacttgta atagaatact attttataac aaaggggatc gaatcaatga
    41341 aaaaaactgt tatttatact agtttatttg cagctttgtt tttagctgct tgtcaatcaa
    41401 ataccaatca aggcacatct gggaatacgt cttccagtag cgaacagcgc tcttcacagg
    41461 tatcggactc ctcaacaaaa gcaagcacat cttcgtctag ccagacaaaa agttctagtg
    41521 aagcaagtac tcagtcagat atcaaagtgt ctgcagctcg tgcgatcgaa ttgttccaag
    41581 ataaataccc agatactgca attactagtt tggaattaga tagtgattgg ggatcttact
    41641 tctacaagat agaaggtgtc gatgatcaaa aagaatacga agcaagaatt gatgctcaaa
    41701 atgaaaaaat ggagacagag aatccagaaa ctctagatcg agatgaacaa aatggtcaaa
    41761 agaaaaaaga agatggtctg gatgttgata aattgatcac gattgaggaa gcagcaaaaa
    41821 tcgccgtcga caaagtagga agcggtacgg caacagattg ggatttggat aaagatttag
    41881 gtactactta ttgggaagtc aaagtaaaag acggcaatca aagtacatca gtcaagatca
    41941 atagccaaac aggcgaagta ctgtcaactg aacaagatga ttaagaattt cttcggaaaa
    42001 caagagttga tttttctttt agtcatgata cactagacaa aataacttgt ttgaaggagc
    42061 gatttacttg agtttagaaa cgacctttgc caatcatatc tttgccaatc ctttgatgaa
    42121 tgcatctggt gtccactgca tgactaccca ggaattagat gaactggctc attcagaagc
    42181 aggagcattt atcactaaga gctgtacgat caatgaacga aaaggaaatc ccgaaccacg
    42241 ctattttgat gttcctttag ggagtatcaa ttctatggga cttccaaact taggtttttc
    42301 ttattatcta gagtatgctc ttgcgtatga aaaagtgcag gaaaaccaaa atcaaccgtt
    42361 atttttttcc attgccggga tgagtgtcca ggaaaacttg gaaatgcttg agaaaataga
    42421 aaaaagtggg tttaatggga tcactgaatt aaatttatct tgtcccaatg tgcctggaaa
    42481 accacaacta gcttacgatt ttgaggccac ttatgaaaca ctgaaagaag tattcagcat
    42541 cttttctaag ccgctaggaa tcaaattgcc tccttatttt gattttgctc attttgatca
    42601 gatggctgat atcttgaatc agtttccatt gacctatgtc aatgctatca acagtgtggg
    42661 caatggtttg tatatcgata cagaacaaga agcagtagtc atcaaaccaa aagaagggtt
    42721 cggcggcatt ggcggagagt atatcaagcc gactgctttg gcgaatgttc gcgcttttta
    42781 tacacgtttg aaaccagaaa tccaaatcat tggtacaggt gggattcgga caggacaaga
    42841 tgcatttgaa catctgctat gtggcgcaag catgttacag attggtaccg agcttcataa
    42901 agaagggccg gaaatttttt ctagaatcat aaaagaatta acgcagatta tgtcagaaaa
    42961 aggatatacg tcaattgacg aatttaaagg caaattacgc accatttcat agcaaagaga
    43021 cgaaggtcat gattgatctt cgtctttttt tataaaaaac actcgtctgt tcattcctgt
    43081 gcaagcgtac tcattgtatg ttagacgaac ttgtgggaaa ataacagagg caaaaaggag
    43141 gataatatga atgatgcaac agaattagca aaagagctta aagaaaaaga atttagtttt
    43201 caagaactag ttacggatat acagaaaaaa ataaagaagc aaaatcatga attgaatgcg
    43261 tttgtcgcgc ttgaaccatt cgagagtaat tcccacgagc tattgattcc ccagtcagaa
    43321 aaagggccat tggctggtat cccatttcct ttaaaaatgc tcggtcaaga aaaaaaggga
    43381 tggctagcta cttcaggatc tcgtttattt gaaacacacc gagcttctca tacatctaat
    43441 tatgtgcggc aagcagaagc gataggtcta gttccttttg gacaaaccaa tgcgccagag
    43501 tttggtttca aaaatattac agacccagtc atctatggcc cagcaagaaa cccttggaac
    43561 cttgaatgta caccgggcgg atcaagtgga ggagctgctg ctgcagtttc atcaggaatc
    43621 gttcctttag ctggtgctag tgatggcggt ggttctatta gaatacccgc ttcgttttgc
    43681 ggattgattg ggctgaaacc ttcgcgaggg acgatgccag ttggtccata tgcttggaga
    43741 ggatggcaag gagctgctat tgatttcggc ttgacagttt ctatgagaga cacggaggct
    43801 ttattttatg ggatgagaag tatccattcc ggtgcgccat atcaagtatc gccagttgag
    43861 tggcaaacac atccaagaaa aaaacggttg aagatagctt tttgtgttga ttcaccgatc
    43921 cactcacaga ttagtgaaga ggcagtactt gctgtaaaaa aagctgtaga tttccttgga
    43981 aaagaagggc atgatttgac agagattcct tacccactag atggtcgtaa actgatcacg
    44041 tcttattatc agatgaatgg cgcagaaaca gcagcaatga tccgctcgat tgaaacaggc
    44101 atccagcgag ctgtcagaaa agaagaactt gaacccattt cttggacttt gttccaatat
    44161 ggtcagaagc tctctgccgc tacttacatc gattctctgc atgtatggga tgaagcagca
    44221 attgtcatgg aaaacctaca tcaagaatac gatctattcc tttcaccaac gactgcattt
    44281 accgccccta aaatcagcga agatttgcaa agtgatgcga taagagcaca gatggaacac
    44341 atcagtgatt gttctgaagt agaaggttta gacgttgttt cttccatgtt tgaggagagc
    44401 ttgaagatca caccttatac acagttggct aatctgactg gacaaccagc aatcagcttg
    44461 cctactcatt tgacaaaaga aaacatgcct ctaggaatcc aatttatggc tgccagagga
    44521 agagaagatt tgctatttca aatcggctat ctatttgaag caaatcatca gttccattta
    44581 ccaaatccat ggaaatagca gaaacaagga gaagaagaat gaagaaaaaa agattgtttg
    44641 ctctaataac tagtgccttt tttattttaa gcgcttgcgg tacagctgcc gctaatcagg
    44701 aagataacag ctggagtaat gtagaaaaaa agaaagaatt agtagtagcg acttctggca
    44761 cactgtttcc cgcatcttat tataatgatc aaaatgaatt agttggttat gatgtagatt
    44821 tggcaaaaga ggtggctaaa cggttaaatg tcaaaatctc ctttaaagaa tacaacgtgg
    44881 atggacaaat cacttctgtt tcaaaaagag aatcagattt tgctgcgaac gattttggta
    44941 tcacaaaaga aagagaaaaa aaattctccc tttcagagac tttcaaatat tcatttgaca
    45001 gtatgatcgt acgaaaaaaa gatgattcag gaatccactc acttgaagat cttaaaggga
    45061 aaaaagcagc tggcgaaccc aatactagct atatgcgttt agcggaaaaa cacggagcta
    45121 aattagtcac atatgacaat gccacgaatg atcagtattt aagcgatgta gcaaatggtc
    45181 gaacagatgt tattctaaat gattattatc tgcagaaaat ggccgtatct gctttaccag
    45241 atatccctgt aaaaatactg gagaacgttt atttcaatga aaacgaaagt ggttttttat
    45301 tgaaagaagg aaatgatgct ctgaaaaaga aaatcgatgg cgtattaaaa gagatgaaag
    45361 aagatgggac gacaaagaaa atatctgaaa aatattttca aactgatgtt tcagtcaaac
    45421 cgaaagaaac gatggctgag tcggtgagtg agtgatgtac tttcctctaa ttgatttttc
    45481 cctcatgtgg acttcacttc ctttcgtact gcaaggcttg gtatacacat tagggattgg
    45541 cttcgtgtcg tttcttttag gaaatctggt agggttgctt ttgacagttt taggtctttt
    45601 ggattggctg cctctgaatg tatttattcg tttttattta tcgttcttta gaggcattcc
    45661 cgcactagtc ctgctgtttc tgttatattt tgggttacct tatcaattaa gtgccttaac
    45721 ggcttcagtc atctgcttta cgattaccag cagtgcattt attggtgaaa tctatcgagg
    45781 atcactagca ggcgtttctt caggtcagtg ggacgctggt ttagcttctg gtttttcttt
    45841 ctttggggta ttgaaatata tcgttttgcc gcaagctttt cgaatctcag taccggcact
    45901 cagtaacgta gctatggatc tagtgaaagg tacttcgtta gcagcaacaa tcacagtgcc
    45961 agaaatattt caaaaagcaa agatcatagg cggtcggaca ttcgattatt tttctctcta
    46021 tattttagtt gcactgattt attgggtcat ttgtatcggg atcgaaaaag tgcaacagta
    46081 tttagaaaaa cgttctatgc atcagtcaat ctgaaaaata gaaatggtgc tagcgtggct
    46141 ggcaccattt ctattttttg tttcttgtat attctgccta gtattttaaa accatttcat
    46201 gtaaaatgta aatcgaaaat atgttcggag gtagaaaaca tgaagacccc gctaaccatc
    46261 gaaatagaat cctcgttata ccaatattgc atcgagcaag gcggcttggt tgtagaagaa
    46321 gtcacgatgc ctgatgacca aggaattgta gatacgctta gctgcttgac aaaatttgat
    46381 ggaagccgag aatggcgatg ctatgagctg aaagtgacaa aagcagattt tcgctctaca
    46441 gccaaactgt catttgttgg acattataat tattttgttt taccaagtaa actatatgaa
    46501 gaagtaaaaa ctgaaatccc tacaggaata ggtgttttag tgtatcgtcc gtacacggtt
    46561 gaagaggaga tgcctgcctc tggtacattt ctgattgcga aaaaagcagc aaagaaagag
    46621 cttgctgtac cggaagaagc tttgacaaac cgcttcatgg cttctttatt cagggaagta
    46681 agaaaagcaa agcaaatgga gcgaggcgta aaatattttc caacagatca attgtacaaa
    46741 gaattgaaaa gacgttcaga aaatcaagat ccattttcta acgaacgata ttatgaacgg
    46801 tttttagagg aaatagctag tcaggcaatc aatgagttgc aagaagaagt tatcgcgctt
    46861 cagcatgatt atgaatattt gaggcaacaa cagcagatcc gtcggttgcc tacagagcca
    46921 ttggagtgaa aaatatgtac tatccttatt taagaggaaa acaatttgac ctattagcat
    46981 taaaagaagc actgtcgcga ggattgctct ccaataaaat ccaaccagtg atcgaaccag
    47041 tacgtgattc cgctacgctt aaaaatgtga tcgaattatt tcaaaaaaag caccatcgaa
    47101 tcgctgtgat ccaaaatccc caagtaggac aattcaaatt attcgatcag catgtgcaca
    47161 gttggacagt caaagaaaat agctctgtcg tccctgcaca gatcttaacg ccagaaaatc
    47221 tcagtgaagt actggattca cctccagcgt ttcttgtttt cgatggacag cactacccaa
    47281 aagatacaga ggtgtggaaa cagttggcag gagttgattc gaaatttttg ataccagata
    47341 ctagtcgctt tcgcatctgg ctgcccgaaa acaaaattgt gatccgtgac tcttttcaaa
    47401 cgagaaagca tgttgaaagt tatgcagata aaacagatga cttttttagt gatgactatc
    47461 tttttttcca cgcagacgga tatattggat tttctgattt cacgatcgaa ggtagccgtt
    47521 atttcgataa agggttccct agccgagcgc ttgctatcca tctaacgtat attgatgctt
    47581 acggcaatat acgcgtgaaa cattttgtct ctgacagtaa cgactctgct aaagaccaag
    47641 cattgaagtt tcttgaagca ggagataaaa tgaaaaaatg gatcatgcgt caccatcatc
    47701 aactcttgat tacttcagga atgattgaat tattggctct ttaccagcaa caaaaattcc
    47761 ctggactggg agtgttgaaa aagtggtcac ttatgcatca tttggaactt attagtcacc
    47821 tgctagatca tccaaaagat tggcttaaat attattcaaa agttcaggaa ctatatgaag
    47881 tgatccaatg attagataat gtaaatgaaa aaggaataga aaagaggaaa ctggatgtat
    47941 attcggcaaa caaggaaaga agaagtagaa gaagtggtag aaatcattaa tgccgcagcg
    48001 gatttcattg cttcgcaagg ctctcctcaa tggcaaaacg ggcagaaacc aacaaaagaa
    48061 acgattttga acgatattga aagaaaagaa agctatgtat taatagtgga agatcaaatt
    48121 gctggaacag ctgcattagt ctcaagcgtg gatcccgttt acacaaaaat agatggtagt
    48181 tggaaagaat caactggtcc ttatttatcg attcatcgtg tcgctttagc agaaagtact
    48241 cgaggaaaaa aactagcaaa accgttctta gaacatttgt tgacgattgc agccagcaaa
    48301 aatatccaag atatccgtat cgatacttat ccagctaatg aaccgatgga aaagaccatc
    48361 tattctgctg gattcactta tcaagggatg attgaatttc cttttgaaca cggcgaaaga
    48421 aaagcatatc aaataatcat caattgacta taaaatcatc tgagcaaaga taaagacatt
    48481 aacactactg aaaaaaacaa tcatatgata gccaagaggt tgtaacagaa gtggccagct
    48541 tcaaaaaaca agaaagaatt tccgaaaatg cttctcaaat ttttgtggaa ttttagcgta
    48601 ttttacgaaa tagctgcttt ctgaaactcc cttcatcgag aggtcgtgac atagcttttg
    48661 tcacgacctc tcggcttatc ctacgagtat tattcgatac atttatatca ataatccttt
    48721 ttttatttta tctgcagctt cttggaacgc tccatcgttt gacacgacta aaagtgaggg
    48781 gatagctttc aaacgtttca actgtgctaa atcttctaca taaaccgcca gtctggctga
    48841 aatctgttca gatgaatctc cacgtttttt caggcgcgat tgcaatgttt ctttagagat
    48901 cgtaataaag ataggacgga ctaccgtccc gaaatactca tataaatgcc aaaaaccatt
    48961 tgctgtcaaa acggcgtaac aatttcctac tgtcagtttc tttttaattt ctgctttact
    49021 ggaaccatat ttatgttgag catattggtc ccactctgca aactcatttc ccttgatcat
    49081 ctgattgaat gtattgtcag aaacaaaata ataatcctct tgatcttttt ccccaggtct
    49141 tggtgatcga ttcgtgtaag taatgatctt ttgttccggc tgaaaaatag cttctgcgag
    49201 tgaagttttt ccagaaccag aaggaccgat aaatacatag caataattca ttgtcatttc
    49261 tcctttgatg attgaacaag tgaaaaagct ttggaaaata tttccaaagc ttttaaaagt
    49321 aactttgctt ttttgtatat tataccagca agccagtcac aatagcactt aaaaagctga
    49381 ctaaagtcgc tgttagcaaa attttcaatc cgtgttttgc gacaagattt cctttttctt
    49441 catttaatcc cttcatcgca ccagaaatga tcccgataga agagaagttg gcaaatgaaa
    49501 cgaggaagac agatataatt gctgttgtac gctcagtaaa atggaaatta ccatttgcta
    49561 attctgtcat agcaacaaat tcatttgtaa ccagtttagt cgccatgata ctaccggcat
    49621 ccactgcttc tttccatggg attccgctga tgaaggcaag aggggcaaag atgtaaccta
    49681 ggatctcttg gaaagtgatg ccgaatatcc cgtggaagat agcattgatc atggcaatca
    49741 aagcaacaaa accgataagc atagcagcaa ccgtgatcgc cacatggaaa ccatcaagaa
    49801 tgtattctcc caacatttgg aaaaatgtct gttctttgtt ttcttcaatg atcagctcat
    49861 cttctttttc ttctaataca tagggattta caattgaagc cagaatataa ccgccaaata
    49921 agttcaaaac aagggcagtg atcacatatt tagaatcgat cagtgccata taagatccga
    49981 cgatcgacat agaaactgta gacatagccg atacactcat ggtgaacaaa cgtttctcag
    50041 ataggaaggg taattccttt tttatggaaa taaaaacttc cgattgtcct aaaatagcag
    50101 aagctactcc attataagat tccaatttgc ccatcccgtt gattttactt aatcctagac
    50161 caacagcttt cataaataat ggaagaatct tgatatagcg tagaataccg ataatagcag
    50221 aaataaatac aattggcatc aatacggaaa gaaagaaagg tgcacttcct ttatttgcta
    50281 caccggcaaa aacaaagtca acaccttgcc cagcaaaagc aagaagatga tcaaacattt
    50341 ttgccaatac agaaacaacc gctgtaccaa aagttgtacg taataatacg aaaccaagaa
    50401 caagctgtaa aacgaacatc acaattagtg gtttcaattt gattgctttt ttgttactgc
    50461 tgatcaaaaa agcaagccct aatgtgacaa ataaaccaat aatacctaat aaataactca
    50521 aataatcact cctattccaa attccgtttt attttacctg ttttgcaaat aaaatactaa
    50581 tctttagtga aattttcatt acttttttag agtgaaacgc attcattttc cattagaaaa
    50641 taaagcgaac aaaataaaag gtttaaaaag tgtaacgtct tagtaaagtc tataaaaaaa
    50701 cgaaactatc gattaagcta atagtttcgt tttcaacatt tataagtaaa ctttttctat
    50761 tggtaatata gattttcgga agggtagaca ggtgcgcagg taatatcgat tcctagtttt
    50821 ttcaatgttt gttcatcatt tttattgacc atcacagtag agtgggcttg tgctccctca
    50881 agttcagcta gtttatcata agcaacttga gcgattggat tcgttactgc actgattgat
    50941 aaagcgatca agacttcatt gatgcttagt gcatccaaag aggaatgaag tgtctttgat
    51001 ttcaatcctt gtattgtttc aagaactaat ggtgacaata aaggaatgtc atctgcgata
    51061 tgggctaaat gttttactgc atttaagata actgccgctg acgcatccat caatgaactt
    51121 gtacgcccgg taatgatgac ttcatctggt aattcgattg caatcacagc agaaggatct
    51181 tttgtaggac tttgcgcttg aatcttatct gcatagtcat gggcagggac taccacgttt
    51241 cggtcttcag gacgtaaacc gacttcttcc ataatcagtt tgattcgatt aactgcttct
    51301 tcgtcgatca atcctttttt gaagtcgcat tctgtttgga aataacgacg gatgatttct
    51361 tgtttagagg cttcttggac aacggtatca tccacgatcc caaatcctac acgattcaca
    51421 cccatgtcag taggagattg ataaacagat tctttaccag taattttttc aataatacgt
    51481 ttgattacag gaaatgtctc aacatcccga ttatagttca cagctacttt attgtaggca
    51541 tcaaagtgga aggaatcgat catattgaca tcttttagat cgactgtggc tgcttcataa
    51601 gcaatgttca gcggatgttt caacggaaca ttccatactg ggaaagtttc gaacttggag
    51661 taaccagctg catgtccttt ttggctttca tgatacagtt ggttcaagga agtagcgagc
    51721 tttccacttc ctgggccagg agctgtcact acaacgattg gtttagttgt ttcaatatac
    51781 tcattttttc caaaaccatc ttctgagacg attttttcta cgttgactgg atagtcttcg
    51841 atttctgagt gtttatagac cttgatgttt cgtctttcta gtttattgat aaagactttc
    51901 gtggcaggct gtccactata gcgagtgata acgacactgt tgattaataa gccataacca
    51961 cgtaattcat cgattaaccg caagatatcc atatcataag taatcccata atcaccacgg
    52021 atcttattac gttcaatatc tccagcatag acgcaaatca gtatttcagc ttgatctttc
    52081 aatttttgca gcaatttgat tttcgcatct tcttcaaatc ctggaagtac acgtttcgca
    52141 tgcttgtcgt caactagctt cccaccaaat tctaaatata atttgtcata gtggttcact
    52201 cgttccaaaa tatacgctga ctgctcttca atatattttt ctgaatcaaa accaattttt
    52261 ttcactaggt tccctcctta agtactaaag cattttttca tttttttgag gattcgtcaa
    52321 ctggaagagc gaaattcctc aaaaaaacaa agaaaatggt ttaaaccaac ttgtctagat
    52381 agttattcat tggtatgata gtttttgata aggaaggtgc tacatgaaaa aaagacagaa
    52441 aaagaaaaac gcatataaac actatatccg ttcgattttt accggttatg aaagaatgct
    52501 tgaagatccc gaattggaac aattgacgtt tacttatcta aacgaagaaa cgcagctaac
    52561 tcgtgatgag catcagcgca ttcatttcac aacaagagat ctgcctagta aataaaaaat
    52621 aagataaagg tcatcggatt cgattctgtc ctatcagatg gaaagactag ttgatcgaaa
    52681 aaaaggctgt accaaatttt ggtgcagcct gttttatgga gcattgcccg ctctttttta
    52741 taacaatttt ttacagaaaa tggtactatt gaagtattaa taagtgaaaa acagatgagt
    52801 aagaggaggc aagtagtatg aaagagccag atacaaatgc aaattatgag ccagacgaac
    52861 tagaggagac gaatgcggat caggatacag gacaagaatc gtcttctttt tacgatatca
    52921 aagaaaaagc aagcatgaat ctttcccaat tattagaaga cttacaggct tttgaaaaag
    52981 ctgtccgcga agaagatatc cctgagatct accgtttata caatggccga ttgaatgaag
    53041 aattaaaaag aagctcaaat gaaaatcacg agatcgatca attgctgatg cgtaagatcc
    53101 atgatcgttt tttacaggaa tttcctttta tggaacaagt agaaaacatc tcgccaacga
    53161 tgagctatta caaaatcggc acttattacc atgatcggcc cacggtcggt attgatgcaa
    53221 gtctgccaga aatttttgtt ttgcctaaga tcgatgaaga gtgggagaaa tattcccaac
    53281 cggagtcaac ggactatgac aaaaaaatca atgagctaga cgcaaaggtg atcactgctc
    53341 agacagaaat tgaacgttta gatgaacaaa taaaagaaat caatcgacaa atgactgatt
    53401 tgaacgacaa taaaggattc ttgaatcgta aaaaaatcga ggaagaaatt caagaattag
    53461 agaagaagaa gcaagtacta tctaatgaaa aattaggctg gcttccttat attgagacgc
    53521 ctgaaacaat ccagcagcaa aaagaagcat tgaagcaaga agcaagagca gaccagcttc
    53581 gtgcagccat cgtagaaaaa gaacaaaggc aaatcagacg ctatttcggt agcaaagagg
    53641 gattcggtca agcaatccat gaattcttga tgaattatct tggcaatgag aatcctaata
    53701 atcaatctaa tgaaggaggt tccgagtatg aatgaacacg atgaagcaga atggacaaaa
    53761 aatgaagaac aaacagaaga tcatgaagaa acgaacactt cattggaaga acaaacaagt
    53821 aatcctctgg atgacacaga agaaatccct atgctagagg aaacaaaaat acacgaaaaa
    53881 gaaaaagtaa ctccgacgaa tacgaatact gcagaaaaca cccatgctta cgaaataact
    53941 gaaaatcccg ggtatttaga aggaaaagag caagatcatc aagatatcgt cagtttgatt
    54001 gaaacagcgg aagcagaatt agcggattta cgtatgagaa aagatttgat cgaatcagat
    54061 tttccacaat tattagaatc atatgatcgt tttatattga atgaagaacc aatctccacg
    54121 atcggaaaga caagcctgat cgaatacttc acgtatgaat tactgaaacc gttgaatgat
    54181 attggtctgg aacggtcaaa ttcagcttat gacacatgga aaacaagaca tttttcgatc
    54241 aattatacgt tgaatggaca aaacgaatta gtcttttcct ttgtattacc gaccatcgat
    54301 caacgatacc aagtggaatc gatgagtctc ctaaacgtat caccagagac aatggaaatc
    54361 aatgtccaag atgaccgggt gctttctttg atccgacact ggtcagtgga tcgagtattc
    54421 tctgcagggc agatcacgat attcaatcac aaattgaatc aattattagc ccatgcaaga
    54481 acacttggtt ttaccgttaa tcagacatta ctagacaata cgaagccact tcgtttgaat
    54541 cttcagagcg aatttgaatt gacgaatgaa gtattggatg atatttttat cgtggcaatg
    54601 aaaaatccat cctacgatat ggaaaagatc aacgaaacta cttatcaagt acgattagat
    54661 aaaggacagt ctttaacgat aacaacaaac gaaaaagatc aaacagtctt gtcgatctca
    54721 tctggagact acccacgatc tgtcattgat ttctttatca attacgaatt cttggtccca
    54781 ttaatggtga gaaagtcctg attggataag ttggattacc agatgtcatt cagataattc
    54841 tgttccaaag gacttgcggc agttatccag aataattgtc acaagtcttt tctgtatgta
    54901 ctaaatggac gtgaataact tgctgatgaa ggagggattt cattaggaat gtccacaaat
    54961 aaaatagcac agcttagctt attgtctgct gcttgtgtag caggtaggat cgcttttcaa
    55021 tttattccga atttccagcc aatgactgct atttttttat ttatagtact ttacttaagc
    55081 ttaaaagatg cgttaattgt catgagtctt tctattgcga tcagcagttt ttattttggt
    55141 gttggaccat gggtcatcgg ccaatggatc agctatactg ttgttctgag tttgttttca
    55201 atcgtctgct tgagaaaaac agtgcaaagg aatctatggt taaagggagt ctgttttttt
    55261 ctggcgggca tggtttacgg aataggtatg accgtttttg atacgctgtt atatcgtttg
    55321 cctcaacctt ggatctatta ccttcaaggg gtgtcgtttg atttgatgca cagtataggc
    55381 aatgttgtgt tctttcttgt ttttctgcca gtcgtcaaac gtttttacaa tcattcagga
    55441 ggaaaaaatg aaaaaaataa tttatagttt gcttgcaatg agtgtcagta tactcatttt
    55501 gacaagttgc agtacaacct atacacaatc ggcttccaac caaaaggagg aagtaaaaac
    55561 atcgcaaaaa gcaacaatca cgttacaaga aaatggtcaa aacttttcgg aaaaagaagt
    55621 gttcttcaaa aaaggcgatg atttactgag tattttgaaa cgaaattttg aagtcaaaga
    55681 agataacggc ttcatcactt cgcttgaagg gcactcacag gatgagaaaa atcaactcta
    55741 ttggatgttt actgtggacg ggaaaagtgc tactactggt gccaaagaga tcaaattgaa
    55801 tgatggacaa tcggtcattt tcaatttgtc taaactataa gtttcataaa aaactgttaa
    55861 agggcgaata ttgccataat tcagtgagtt cagttgatga aagaaggaaa ataacatgga
    55921 aaattttaaa aaaagcggaa tgttttccgt aattgctgct ctaggggcta atattctagt
    55981 tgctatcagc aaatttgtcg gctatgcaat cagtggcagt gccgccatgc taaacgaaag
    56041 catccacagt atcgttgatt gcagcaatca gattttgctg ttattcggtg ataaacaagc
    56101 caataaagga cagagcgagt tgcatcaatt tggtgaagga cgagcaaaat actttttcag
    56161 tacaatcgtc gcaatgatgc ttttcttcgg cggtggggca cttggggtga tggaagcttt
    56221 tgaaaagttg acgcatccat cccatgaagt gggtaatccg atcattgtta tagcaatact
    56281 gttatttggt ttggtaatcg aaggaagttc gttacgcgtg gcaatgaaag aaatcaaaga
    56341 attgaatact gaaaaacttt ccacgatgcg ctttttgcgc gaaagtcgac acagtgaaat
    56401 actgattatt tttacggaag acttgtgtgc tgtaatcggt ttggtcttgg ctttagcagg
    56461 aacaatattg acttatgcaa ccgggattgc tgcatttgat gcgatcagcg gattgctgat
    56521 cggtttcttg ctgatgggag ccgccatttt cctagcaaaa gaattttata gtctgatcat
    56581 cggtgaaagc gtgacagcaa gcgatttatc aaaaatcaag ttagcttttg aacgtcctga
    56641 gatcagtcgg ctgatcgata taaaaacagt tcatttaagt ccaacggaga ttttagttgc
    56701 agcaaaagca gatatcgttg gtaacaaaga gaatgaagct tactcggtca tcaatgatat
    56761 tgaaaaaaat atgcgtcagt ctcttcctga caagaagatg tatatataca ttgaaacaga
    56821 taaatatgac ccaaattatt ctcgtaaata aaacgtttat tcatgaattg aagcaaaaat
    56881 gagaaaaata taatcttgtt tttaaataaa acggctacaa tttagcgaat ttatgttaca
    56941 atgagtccgc tgtttatttg ttgtaattaa aggagatatt ttaatgaaca aaaagaaaag
    57001 tgcgccatgg cttcaagctt tgcatgcagt gatgccactt tgtataagct atataccagt
    57061 aggattagct tgcggagtat tgttgcaaaa agttggattt gatcctctct tatcaggact
    57121 gttatcgatt ctggtctttt ccggtggcgc gcagttttta gttgcatcta tgcttacgac
    57181 gcaagcatca tttgcaacga cattattgat ggttttcttt ttagaattac gctatacatt
    57241 attaggagca agtcttgccg gttttatgaa aaaagaaaag cggacgtttt tggctgtctt
    57301 ttctcaatca ctgaatgatg aaaactatgc ggtgaactac ttaaagtttt caactgaccc
    57361 ttcctggaat aaaagaaagg ctttatatgt caattggttc tcaatgactg catgggccgg
    57421 tagcaatatg atcggtaatt ttttcggctc ggtgattcga gtagatgctg accttgtcca
    57481 ttttgctttg acggccatgt tcattttcat gtttatcatg cagatgaaaa gcgcactact
    57541 gatctttaca ggtttattct caggcgtatt aggggtcgta ttcatggtat tattccaaaa
    57601 tactttcgga ttgattgcag caacagtcat tgcttcattt gccggattta tgctggagaa
    57661 ggcattcaaa aaagaaaaag ctaagaaact aagagaccgc ggtaaagatg tcaatgagcc
    57721 gctcttcaac ttcccggaac aggaggccaa tcaccatgac taaactatat ttgctacttt
    57781 tagttgcttg tctgttcatc gtggcatata tcccgcgctt gttcccgatg ctttatttta
    57841 ctcatcgaaa agtgccatcc tggttcagtg actggatgaa atatgtaccg gtggcattat
    57901 ttgcggcgtt agcttttaaa gatgttttca ttactcatga acatctggat attgcttgga
    57961 atatcaaaat agctgcaatg attctagtag caggcgtggc ttacaaaaca cgttcgatgg
    58021 cgttgtcagt tttgacagga cttgcgtctg tctttttgct gtctatgctt taaactgttt
    58081 tattttatta aaaacagaga ttgcgacata agttgttcaa actgatctat ccgaactgca
    58141 tttgcaacag tcaattctgg atgcaagttt attcgtaggg ataaaaaaga ttagtgttgg
    58201 cttatgatgc aagctctttt ttattttagg aattagagag ggatttgtcc ttctatgatt
    58261 ttttgctata atagggaaga tgtttatttt atgaaagaat agttgaggga atttaaatga
    58321 ataaaaaatt gatgcaagga acattttggc taacctttgc taatcttcta tgtaaagttt
    58381 taggggtagt ctatttgatt ccctggctaa gtatgatggg gaacaatcaa gatggtatgc
    58441 ttgctacgac attgtataat gttggctatc ttccttatgg attgttcctg atgcttggta
    58501 cagtcggatt tccaaatgcg attgcaaaaa aagtagctgt agccacaaaa gaaggagata
    58561 acgctgcttg tcggctgatt tttagaagta cgatcaatat catgtttgtc atcggtatcg
    58621 cctctgctgc actgatgtat ttatttgctc ctttactagc cggaatcagt ccgattgcta
    58681 acgttaataa tgggatacta gctatcagaa gtttatgccc atctttgatt gctatcccaa
    58741 ttcttagtgc aatgagagga tatttccaag gaaaaaatga tctgcggcct tatggaactt
    58801 ctttgattat cgaacaggct gtgcgagtga tcgttatctt ggctggtact tattacttgc
    58861 gcgtattgac tgacggaacc atattagaag ctgtattgat cagtacagtc gcttctttct
    58921 tcggtgggtt agctgcgatt atccatatgt atatcgtcgg acgcagaaaa gacttcttcg
    58981 aacttaaaga ttttttgatt tccagccgtt atttcgaaag agaaaaccga tctgcctctg
    59041 tctctatcat tcgggaaaca ctaccgttta tttttgtcgg ctcagtcatt actatcgttc
    59101 aaatgatcga ccaagtaacg atgaaaccac tgcttcactt tttccgtcca gagatcgcag
    59161 atcagcaact ggaattctta ttcagccgtg cttctgtcaa tccaaataaa ttgacattga
    59221 tcctgatctc catggtagga acagtagcta taagcagctt gccaatatta agcacgatga
    59281 agaaaaaaga tcggatacaa atcgaaaaaa cagtaggaga tagtttttcg attgcgttat
    59341 tgattcttct tccttcattg actggaatgt ctttattagc tggaccacta tacacattgt
    59401 tttttggcta tgatcctgaa agtgtcggat atttccaaat ggctttgcta gcttctttgt
    59461 ttttctcttt attcacgatc ctatctacga tgcttcaatc tttgaatcac catcgttttg
    59521 cgatccgatt aactgtagaa gcaattatcc tgaaagtcat cttccaagta atcggattag
    59581 gattagttgg cggatacggg atgagcttat caaatggctt agcattcggt attgtctttg
    59641 ttcgcggcta tcactttatg tgcaaagaat accgaatagc acctttagca aagatcagca
    59701 attttttctt aaaaactttc cgctccaccc tgattatgct tgcagtatgt tttgccgttt
    59761 tctttttgct gaatcagcga ttgtcaatgg aatcaaaaag ctatgcaatg atttattgtg
    59821 tggctgtagg aagtattggc ggcttagtat ttttattttc tcaattcgga aaaaatgggt
    59881 ttcgaatgtt gaaaaatgtt aaacatcata gcagaagcaa agaataggtt aactaatcgt
    59941 tttttgctgt cacttgaaaa acggcaactc gtatgattac gcttgtcgtt tttctatttt
    60001 ccaattttag tttcatatct aatcttactt atgatatagt tataaggtct ggaaaaattc
    60061 tttgaaacga ggaaattaaa cacatgaaac ttgcagtagt tacagatagt tcggcatatc
    60121 tgccggaacg tatcaagaac catcccgact tattcgttat tccaatccct ttgattatgg
    60181 atgggaaaat ttataacgaa ggaatcgata tcgaagcgga tgaatattat ggacttttaa
    60241 aaaacagtaa agattttcct actacttcac aacctgtcgt aggggaagtt ttgggtttat
    60301 atgaggaatt gaaagagaaa gggtatgaca cgattttaag tatccatctt tcttcaggga
    60361 tttctggctt tgtcaacaca ttatttgcga tgaaagatga catcagcgga gtaacggtca
    60421 ttccttatga ctctaagatc actagtatgc cgatgggaca catggtagaa gctgccttag
    60481 acttaaatga tgaaggtcag tcccttgatg aaatacttgc acatatcgat cgaatccgag
    60541 ataataccta cgcttatctg attgtagatg atttgaacaa tttagtacga ggaggacgtt
    60601 tgacaaatgg tgcagcgctg attggcagct tattaaaaat caaaccgatc ctgacatttt
    60661 ctgaagggaa aattgtttta ttcgaaaaaa tccgttcaag taaaaaagca tttgctcgag
    60721 ctgaagaaat tattggtaga agaaataacg aaatacaacc gccagtcaaa ctttacgtga
    60781 tacatgcaaa taatttagaa gtagcgattg cggagaaaga gaaattgcag aaaaaatatc
    60841 caaatgcaga aatcgaaatt ggtcatttcg gaccagtcat tggtacacat ttaggtgaaa
    60901 aagccatcgg cattgccatt tcagcgcaat aattcctttt attcaaaaaa cataccaaaa
    60961 atattccaac atcatcttgg actttctaca tgttactaat gagcgtgtaa aagaacgaga
    61021 tggtgttttt atttctatca atgaagctct ttttctttgc aatcctcact tcaatcctct
    61081 ataatgtcgc atatagttta gtaaatgagt aaactactaa tttacttatt tactaaaaat
    61141 aaaaggatga ggagttaaaa taatggaaat accaacaaat ttaaaacata aaccagtaat
    61201 cgttgtagaa gactatgata aagttgatgg aagaaatgca ttaaatacgg atgcaaaagg
    61261 gctttcactg ggccttgctc agtggaatga tcgtggaaaa gtcgatatct caggcaaggt
    61321 atggcgacat actggagaaa aatggtcacg acagtcggaa gaattaccaa tcacacgtat
    61381 tttagattta gcgatcttag ctgcacaagg aagtttatac ttccaagatg cttatcgtta
    61441 tgaaaaattt tatgatccag aaaatccagt cgtagacatc attggattac aagggaaccg
    61501 aatgacggta gaagtagata ccaaaaatcc aaagatcgat gaagacatca ttttgtatta
    61561 cgatacacta caaaaagacg gagaactgat tggggaacga ttccgtattt taaaacgtct
    61621 tttagatgag ctaggatatt aatagacaga gatgatcaaa aggggaagag aagcatggag
    61681 aatttctcac taacagaaaa ccgaaccttt tcacggctag aaaagcaaat gatctggaag
    61741 aaaccgaaaa gccatcggac aagtgcagaa gaatggcgga tcgcctccga aatcaaaggg
    61801 aattggcaag atcctgaaat gaaaatcagt aacgtacttt tggaaggaga tgccgggtcg
    61861 gggaagacac aattagccaa agcactatct gctgatctgc aacttcccta tacaaaagtg
    61921 acctgttttg cagatatgga caaatcagat gttttcggag ccttattacc tgtagtagat
    61981 tctaataagg aacaagacca agaattacta gaagctattt accaaacaga tacacttgaa
    62041 gctgtactga cactgatcca gaaccattac ggcattgatc ctttttctgc taaagaaaaa
    62101 ttagcagagc tagttcaaag gatcgaagct gattatccag atgcaatcca ttacagctat
    62161 tacccttctg aaatcgttcg agcgattgaa aaaggatatt tattagagat tcaagagccg
    62221 actgtcatcc gagatgcctc tgttcttgtg gcacttaact cagcgctcga accaaatggg
    62281 atgttgaatt taccgacagg aatcattcgt cgccatccag attgcgtcat tgtgatcact
    62341 acaaatcgaa attaccaagg aaatcgccca ttaaatgaat ctttaagaga tcggatgcaa
    62401 catgcggaaa aaatggatct tcctgcctta gaagtcatgg cagaacgtgc gatggcgaaa
    62461 actatgatcc aaaaacaaga acttctctta aagatggcag agattatccg tttgttggat
    62521 gaaactgcaa aagctaacgc catcaaaggt gtagcaggaa tgcgttctta tttttattgg
    62581 gcaaatacct ataagcaagg acaagattta ctccagtcga tttatccaaa agttttgtat
    62641 aaattatcca ctgattcaga tgaattggca attcttgaac aagctttgtc tgacagtgga
    62701 ttgctactgg aacttcagga catattgaga aaagaaaaat ggggtcatcc tcaatctgag
    62761 catacaaaag gaagaaccat tagcgccgaa gaagcaaatg aacgaaacat cactgaaaat
    62821 gctgaagaaa ttctgcgctt agatgaaaag aaatcgttgt ctgctgaaga accaaatgag
    62881 ggagaagaga aacaagaaga aacaatagaa aaaaacgaga ttcctcctca aatgcaaaaa
    62941 gaacgcagta gcgatatcga aggaaaaaat caaaaagaac aacaaacagg agatgattct
    63001 caagaaatgt ctgcttctga catggaagca catgataagg aaatcaagaa gcaactgaat
    63061 aaagaagcgc gacaaatcat gaaggaaacg attcatgaaa aagaaggtct catcgttcac
    63121 cgaccagctt tccaaggaaa aacgccggaa gcacgtgagc tacacatgca agtaatgcca
    63181 atagtagaat cacttagccg gcaaatttta gagttactgg ataatgaaca aacagagacg
    63241 taccaaaaag gaaaatacga aggtcagcgg ttcaatgcaa gcaaagtagc atatggtgat
    63301 ttgagaaatt ttgacaaaaa gaacccgcca catgagcaac cttctttagc ggtagctgta
    63361 cggatcgatg aatctgggtc aatgatacgg gatgatcgga tcgaagcggc aaaaaaagca
    63421 gcgattgcca ttgccgaatt ttctaagaaa gtcgatatcc cattgttgat ttacggagat
    63481 acagcagacc gttcgtcaag agaaaagact tctttgtttt cttataaaga atttgaagac
    63541 ggatttcaat ggttaaatga aaaattagtc actatgaaac caaggcaaaa caatcgtgat
    63601 ggtgctgcac ttcgtttagc agcagaaaaa ttaaacagac aatccgcaac aaccaaactg
    63661 ttgcttaata ttagtgacgg tcagccaaaa gcgttgccgg attatacagg caaaaaagca
    63721 aaagaagata tccagtctgt tttgaaagaa tatgaacgtc aaggaatcct atttgtctcc
    63781 gcagctattg gccaagacaa agaagaaatc aagtcgatct acggtgaaag tcgttttgta
    63841 gatattactg acttgaatga atttcccaag caaatgattc aactgattgc ccgctatcta
    63901 tgataaaata aagctcaacg aacgcactta gcagattcgt tgagcttttc gtttctagtt
    63961 accggtaaca aggatacttc taaaatagaa tcggttataa tcaagaggga ccgttaaagg
    64021 agtgaaacga tatgatacaa gaacaaaaat ggtggaaaga agcagtaggc tatcaaatct
    64081 atcctgctag tttcaaagat agcaatggcg atggtcaagg agacatcaat gggatccgtg
    64141 aaaaacttaa ttatttgaaa gatcttggta tcggctttat ctggatcaat ccgatctatc
    64201 aatctccatt tgtcgataat ggttatgacg taagtgacta tcaagatata gaagagagat
    64261 tcggaacgat ggaagatttt gatttattgc taaaagaagc acatgaatta gggatcaaag
    64321 tgattatgga tcttgtcatc aaccatagtt cggatcagca tcaatggttt gaagaatcga
    64381 aaaaatctaa agaaagcccg tatcgcgatt attacatttg ggtcgacggg gtagatggca
    64441 aagaaccgaa taattggaca tcgattttcg gcggttctgc ttgggagtat tctcatgaaa
    64501 ctggacagta ttatctgcat gtttttgcaa aagaacaacc agacttaaat tgggaaagcg
    64561 agaaattgaa agaagaacta ttcaatatga tccgatggtg gttggataaa gggatagatg
    64621 gatttcgttt agatgcgatt tctcatgtaa aaaaagatga atattcagta aaagccacag
    64681 agaacccttt ctcacccttt caaaatgtct caggaatcga agaacatttg accgatttga
    64741 aacatgtttt tgaagagtac gatatcatga ctgtcggaga agcaagtggt gtcactgcag
    64801 aagaaggacc acaatgggta gggaaagacg gttatttcga tatgatattc gaatttgatc
    64861 atatccatat ctggcagcaa gaaaaagaag ggcaattaga tgtccttaaa ttgaaacttg
    64921 cattaagcgc ttggcaaact tcattggatg gtatcggatg gaacgcttta tatatggaaa
    64981 accatgatgt tcctcgtgcc gtttcagtgt ttggagatac tcgtcccgat ttttgggcta
    65041 tgtctgcaaa agcaatcgct atgatgtatt tctttttgca aggtacgcca tttatttatc
    65101 aaggacagga aatcggaatg acgaatatgc cctttgaatc gatcgatcaa gttgatgcag
    65161 tagattcgaa aagactttat aaaaggctgc ttgcggaagg taagacaaga gaagaagcat
    65221 tggatatcat tcgtgaaaca acgagagaca acagcaggac acccatgcaa tggacttcgg
    65281 aacaatatgc tggtttttct acacatgaac cttggctgat cactaatcca aataccaaaa
    65341 cgatcaatgt agaacaacaa gaatatgaac cagagtcggt cctgcaattc tataagaaca
    65401 tgatccgcat tagacaaacc aataaaggtc tgatatacgg ttcttataaa gaatatcttc
    65461 atgaacatcc acaactttat gtctatgaaa gatatttaga agatgaacaa tatcttatta
    65521 tggtaaactt aacagaatca cttgctgact atgaacttcc aaaagaagca gatcagtcct
    65581 ggacattact cttgtcaaat tcctctagcg gagaatttga agctaaaggc atactagcac
    65641 cttatgaagc tcggttatat aaaacaaaca agtaaaactt cctcgttaaa gttcattagt
    65701 tggactttgc gaggaagttt ttcagattgc tctgtcaatt actcatttca ataattcgct
    65761 ctaggaccac cgatatattt aggacgagag cgaagggctg tcacgtgagc ttgtccaagg
    65821 gttttcaaac gaggtctgac aggtacttgg acatgtttga tatgcatacc gattgatgtg
    65881 tcaccgatat cgataccggc ttgtccaact acgtgttcca cttcgacagg atcattgaac
    65941 tggtcaaaag cagcaacaga acaagcacct ccagcatgta gtgcaggaac aacagagaca
    66001 atttcgaaat tttttgcttc tgccactgtt cgttctacta ctaatgctcg gttcaaatgt
    66061 tcacaacctt ggacagctag cgcaatttct ttaggatcca acaactcttt taacgtacgg
    66121 atgatccatt gaccaacttc cgcactggaa ttttttccaa tatgtcctcc aacgacttcg
    66181 cttgtgctgc atcctaaaac aaaaagatca ccttttttca agtgtgcctc agccaatatg
    66241 tcattaacta tttcagtcaa ttggtctttt aattcttttt ctgacaactt cattttctaa
    66301 aaccttcttt ttcaattaat attgcttccg tgcttgaata cgttgaagac ttgcagttat
    66361 aggaaaggca agtcccattc caatcagatt ttggaagatg tttcctggta tagaagccac
    66421 tccagccggc caatcatata aaatccaacc ggcgatacca tagccgatga tcataactaa
    66481 gcttcctaat aaaagcccta atccccacat acgagtagaa gatttgctga aagaaccagc
    66541 aacatatcct tgtaacccgt ggatcactaa tgaaaaaact gcccactgcg gatatcctga
    66601 aatcaaatcg atcaatagac cactggaagc accaacaact aatccacccg ttggaccata
    66661 aagaaaagca gcggtataga tcccggcctc gcataatgtc acaaatccat tcgtagccgg
    66721 tacagggatc aaaattgtta atgaaagggc aactgtcaac gcaatcagca tagcatagat
    66781 cgttaattgc ttagttttca tttctcattc ctcctttttg aataccgcca gaccagacac
    66841 ttccggtatg atcgttcatc agcacgccat tagaaataca atgggcaaca aattttttac
    66901 tttctgctac tgctttggta aaatctgccc ctaacacaag attcgctgtt attgcagaag
    66961 aaaaggcaca accggccccg tttactgttg tagtgtcgat ttttttatga gaaaatacct
    67021 cataatgatc aattgaatag agaagatcca cagcttcatc tccagccatc cttgtgccgc
    67081 ctttgatcac aatgtttttt gttcctgaag cagtgagttc tttagcgaga tggatcattt
    67141 ctgttaacga agagggaaga gcttgaccag aaagtaaagc agcttctgcc aaattaggcg
    67201 taatcatcgt aacaagtgga aaaagttctt cgatgagctg cgaaatatac aattgattga
    67261 tcacagcttc agtttctttg aatgccaaaa cagggtctaa aatgattggg atattttgtt
    67321 ttgtttttat gaagtctcga acaaccatga ttgcttcact tgaattcaat aacccaattt
    67381 tgataccatc taatgaagtc attttatcaa tcgaaactaa ctgtcgttta agcaattcca
    67441 aagatataga ttccagcata aatcgctcat taatatctag tgcccctata gaagtcaagg
    67501 cgcttagtcc gaatagacca aaattctcaa aagtttttaa atctgtttgg attcctccac
    67561 cagcaaatgg atcagaaccg ccaattgtca agattctttt cataacattt tcctccatga
    67621 ataagagtat aacgaaattt tcaaaaaaga aaaacagcca attggcatat ttttatctca
    67681 tccaatttaa aaaaacaaaa agaaaagaaa gaccaaaatg gatctttctt tccttaaata
    67741 gttaggactt ttgtgatttt aattctttct cgatttgaag ccgttgcact ttcattgtag
    67801 ctgttcgcgg gattgcatca aaatcacgga tgatagggac gttcaagcga ggaagttcat
    67861 ggacttgctc ccaccatcga ttccaatcca tttctttatc aggagctaaa gcaataatcg
    67921 gttgtggaga atgattttta tcccttacaa tcactacttc tgccaaaaaa tctaacgaat
    67981 ctaataaaaa gtcttcaata gctaatgtac tattgatcgt ttcgatcaaa tccacttgcc
    68041 gatctttcaa aaataggtgg ccatgcttat ccataaaacc gtaatctccg ctatcccacc
    68101 aatcgccata aacattttcc tggaaccgtg catcttcctt gtaataagtc aaagcccgtc
    68161 ctttagacag taattgtata tgaccatctg tgttttcagg taggacattc ccaactgaat
    68221 ctgtaatccg agcgcttgtc atgtcttcca gtccaacgcc catatctctt gcatcacttg
    68281 ttttcaaact ctctagtgta tgagccttaa gaatcattgg tccgcattca ctttggccat
    68341 atacttgaag aaataccgca tcctgtgcca tcgacgttcg caaaaatgtc ttcattgtct
    68401 ggttgtttat agcatcaaaa gtggagtgat agtatcggat acccgaaaaa gcttctggat
    68461 gctccttagc agtaaaacgc cactgaacaa agttattcgg atgggtctct agtgcaattg
    68521 gccggtgagc ttccaacata tgaacaactt ttgaactttg tgcgtttgct aaaggcatca
    68581 taggaaatcc catggacatc aaagaagaga taccaatatt gaatctggaa tgcactggag
    68641 agatgtgaaa agcgattaat ttcttttccg aaatttttgt gaaaactgtt ttttgccatt
    68701 tcgtccgcca tcccatagaa tgagcagaat gacaaataag tttagggata ccagttgtac
    68761 cagaagtatg ggtcatataa gaaatttcat ttttgtctaa aaatgattga gaaacagaca
    68821 aagcaggttg ttccattaaa taaagaatag g
//
LOCUS       DAFBQH010000008        53024 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.008, whole genome shotgun sequence.
ACCESSION   DAFBQH010000008 DAFBQH010000000
VERSION     DAFBQH010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.008"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..257)
                     /locus_tag="LK694_000565"
     CDS             complement(<1..257)
                     /locus_tag="LK694_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBK5051458.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELK"
     gene            502..1356
                     /locus_tag="LK694_000566"
     CDS             502..1356
                     /locus_tag="LK694_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBK5051459.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            1357..2694
                     /locus_tag="LK694_000567"
     CDS             1357..2694
                     /locus_tag="LK694_000567"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBK5051460.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            2699..2923
                     /locus_tag="LK694_000568"
     CDS             2699..2923
                     /locus_tag="LK694_000568"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBK5051461.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            2925..3815
                     /locus_tag="LK694_000569"
     CDS             2925..3815
                     /locus_tag="LK694_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBK5051462.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            3824..4642
                     /locus_tag="LK694_000570"
     CDS             3824..4642
                     /locus_tag="LK694_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBK5051463.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            4749..5198
                     /locus_tag="LK694_000571"
     CDS             4749..5198
                     /locus_tag="LK694_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBK5051464.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            5216..6898
                     /gene="recN"
                     /locus_tag="LK694_000572"
     CDS             5216..6898
                     /gene="recN"
                     /locus_tag="LK694_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBK5051465.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(6953..7069)
                     /locus_tag="LK694_000573"
     CDS             complement(6953..7069)
                     /locus_tag="LK694_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBK5051466.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(7142..8098)
                     /locus_tag="LK694_000574"
     CDS             complement(7142..8098)
                     /locus_tag="LK694_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBK5051467.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            8269..8637
                     /locus_tag="LK694_000575"
     CDS             8269..8637
                     /locus_tag="LK694_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBK5051468.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            8807..9238
                     /gene="mraZ"
                     /locus_tag="LK694_000576"
     CDS             8807..9238
                     /gene="mraZ"
                     /locus_tag="LK694_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBK5051469.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            9259..10218
                     /gene="rsmH"
                     /locus_tag="LK694_000577"
     CDS             9259..10218
                     /gene="rsmH"
                     /locus_tag="LK694_000577"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBK5051470.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIGLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            10223..10624
                     /gene="ftsL"
                     /locus_tag="LK694_000578"
     CDS             10223..10624
                     /gene="ftsL"
                     /locus_tag="LK694_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBK5051471.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            10625..12817
                     /locus_tag="LK694_000579"
     CDS             10625..12817
                     /locus_tag="LK694_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBK5051472.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            12843..13805
                     /locus_tag="LK694_000580"
     CDS             12843..13805
                     /locus_tag="LK694_000580"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBK5051473.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            13819..15180
                     /locus_tag="LK694_000581"
     CDS             13819..15180
                     /locus_tag="LK694_000581"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBK5051474.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            15206..16294
                     /gene="murG"
                     /locus_tag="LK694_000582"
     CDS             15206..16294
                     /gene="murG"
                     /locus_tag="LK694_000582"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBK5051475.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            16309..17529
                     /locus_tag="LK694_000583"
     CDS             16309..17529
                     /locus_tag="LK694_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HBK5051476.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            17685..19010
                     /gene="ftsA"
                     /locus_tag="LK694_000584"
     CDS             17685..19010
                     /gene="ftsA"
                     /locus_tag="LK694_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBK5051477.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            19033..20274
                     /gene="ftsZ"
                     /locus_tag="LK694_000585"
     CDS             19033..20274
                     /gene="ftsZ"
                     /locus_tag="LK694_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBK5051478.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            20291..20968
                     /locus_tag="LK694_000586"
     CDS             20291..20968
                     /locus_tag="LK694_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBK5051479.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            20982..21581
                     /locus_tag="LK694_000587"
     CDS             20982..21581
                     /locus_tag="LK694_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBK5051480.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            21596..21883
                     /locus_tag="LK694_000588"
     CDS             21596..21883
                     /locus_tag="LK694_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBK5051481.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            21932..22714
                     /locus_tag="LK694_000589"
     CDS             21932..22714
                     /locus_tag="LK694_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBK5051482.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            22735..23442
                     /locus_tag="LK694_000590"
     CDS             22735..23442
                     /locus_tag="LK694_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBK5051483.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            23704..26502
                     /gene="ileS"
                     /locus_tag="LK694_000591"
     CDS             23704..26502
                     /gene="ileS"
                     /locus_tag="LK694_000591"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBK5051484.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHLFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(26551..28077)
                     /locus_tag="LK694_000592"
     CDS             complement(26551..28077)
                     /locus_tag="LK694_000592"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBK5051485.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(28092..28739)
                     /locus_tag="LK694_000593"
     CDS             complement(28092..28739)
                     /locus_tag="LK694_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBK5051486.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(28923..29252)
                     /locus_tag="LK694_000594"
     CDS             complement(28923..29252)
                     /locus_tag="LK694_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBK5051487.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            29429..30157
                     /gene="sfsA"
                     /locus_tag="LK694_000595"
     CDS             29429..30157
                     /gene="sfsA"
                     /locus_tag="LK694_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBK5051488.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            30173..31186
                     /locus_tag="LK694_000596"
     CDS             30173..31186
                     /locus_tag="LK694_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK5051489.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            31186..32463
                     /locus_tag="LK694_000597"
     CDS             31186..32463
                     /locus_tag="LK694_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBK5051490.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            32526..35228
                     /locus_tag="LK694_000598"
     CDS             32526..35228
                     /locus_tag="LK694_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK5051491.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            35380..35697
                     /locus_tag="LK694_000599"
     CDS             35380..35697
                     /locus_tag="LK694_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5051492.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            35727..36047
                     /locus_tag="LK694_000600"
     CDS             35727..36047
                     /locus_tag="LK694_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBK5051493.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            36133..37593
                     /locus_tag="LK694_000601"
     CDS             36133..37593
                     /locus_tag="LK694_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5051494.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            37661..37882
                     /locus_tag="LK694_000602"
     CDS             37661..37882
                     /locus_tag="LK694_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051495.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            37913..38095
                     /locus_tag="LK694_000603"
     CDS             37913..38095
                     /locus_tag="LK694_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBK5051496.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            38095..38508
                     /locus_tag="LK694_000604"
     CDS             38095..38508
                     /locus_tag="LK694_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBK5051497.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            38631..39812
                     /locus_tag="LK694_000605"
     CDS             38631..39812
                     /locus_tag="LK694_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5051498.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVCLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(39883..40047)
                     /locus_tag="LK694_000606"
     CDS             complement(39883..40047)
                     /locus_tag="LK694_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051499.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            40200..40273
                     /locus_tag="LK694_000607"
     tRNA            40200..40273
                     /locus_tag="LK694_000607"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40234..40236,aa:Arg,seq:cct)
     gene            complement(40342..41481)
                     /locus_tag="LK694_000608"
     CDS             complement(40342..41481)
                     /locus_tag="LK694_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK5051500.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(41780..42415)
                     /locus_tag="LK694_000609"
     CDS             complement(41780..42415)
                     /locus_tag="LK694_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051501.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(42528..43163)
                     /locus_tag="LK694_000610"
     CDS             complement(42528..43163)
                     /locus_tag="LK694_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HBK5051502.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(43197..43658)
                     /locus_tag="LK694_000611"
     CDS             complement(43197..43658)
                     /locus_tag="LK694_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBK5051503.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(43788..44219)
                     /locus_tag="LK694_000612"
     CDS             complement(43788..44219)
                     /locus_tag="LK694_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBK5051504.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDK
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(44237..44557)
                     /locus_tag="LK694_000613"
     CDS             complement(44237..44557)
                     /locus_tag="LK694_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5051505.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            44856..45632
                     /locus_tag="LK694_000614"
     CDS             44856..45632
                     /locus_tag="LK694_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBK5051506.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            45647..45850
                     /locus_tag="LK694_000615"
     CDS             45647..45850
                     /locus_tag="LK694_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5051507.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            45866..46204
                     /locus_tag="LK694_000616"
     CDS             45866..46204
                     /locus_tag="LK694_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBK5051508.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            46191..46370
                     /locus_tag="LK694_000617"
     CDS             46191..46370
                     /locus_tag="LK694_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051509.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(46413..46883)
                     /locus_tag="LK694_000618"
     CDS             complement(46413..46883)
                     /locus_tag="LK694_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051510.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            46970..47668
                     /locus_tag="LK694_000619"
     CDS             46970..47668
                     /locus_tag="LK694_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBK5051511.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            47846..48187
                     /locus_tag="LK694_000620"
     CDS             47846..48187
                     /locus_tag="LK694_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051512.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            48180..48851
                     /locus_tag="LK694_000621"
     CDS             48180..48851
                     /locus_tag="LK694_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBK5051513.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            48857..49543
                     /locus_tag="LK694_000622"
     CDS             48857..49543
                     /locus_tag="LK694_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051514.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            49546..50295
                     /locus_tag="LK694_000623"
     CDS             49546..50295
                     /locus_tag="LK694_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5051515.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            50307..50576
                     /locus_tag="LK694_000624"
     CDS             50307..50576
                     /locus_tag="LK694_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051516.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            50581..50745
                     /locus_tag="LK694_000625"
     CDS             50581..50745
                     /locus_tag="LK694_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051517.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            50738..51040
                     /locus_tag="LK694_000626"
     CDS             50738..51040
                     /locus_tag="LK694_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051518.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            51037..51198
                     /locus_tag="LK694_000627"
     CDS             51037..51198
                     /locus_tag="LK694_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBK5051519.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            51195..51500
                     /locus_tag="LK694_000628"
     CDS             51195..51500
                     /locus_tag="LK694_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051520.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            51500..51856
                     /locus_tag="LK694_000629"
     CDS             51500..51856
                     /locus_tag="LK694_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HBK5051521.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            51843..52061
                     /locus_tag="LK694_000630"
     CDS             51843..52061
                     /locus_tag="LK694_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051522.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            52058..52477
                     /locus_tag="LK694_000631"
     CDS             52058..52477
                     /locus_tag="LK694_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HBK5051523.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            52474..>53024
                     /locus_tag="LK694_000632"
     CDS             52474..>53024
                     /locus_tag="LK694_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HBK5051524.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVV"
BASE COUNT        17902 a         8863 c        11045 g        15214 t
ORIGIN      
        1 tttttcaatt cgaggtaggt acggtattca gaaacatcgt tcattaacaa ccggacggtt
       61 gttgtacccc aacgcacaat atctgaagca atctggtgtt tacagtggat gcattcttgg
      121 gctgtataaa tcaagtttcc acgataaata tgacagattt gccctttgta tttttctttt
      181 gtgagacaat tttcgttaaa agtaactgat aagtctagaa tatctaatgt ttctttgata
      241 aggtgagtgt aagacatctt gattcctcct tttgggttgg ttcgccaaat taattctaaa
      301 ggatttcaag gtgtcttttc tatatgtttt ggaaaataaa aaagatgctg gtgtcatcca
      361 ccaacatcaa aaagtataga gccagaatta gaagctgggg cgtaagtcct agcttttttc
      421 tatgaatcat cataaacctg ttataaatcg ggaaaattct atggtagaat gaaaacacaa
      481 aaaatatctg ggagtggtta gatggcagaa ttgattaacg gaaaagaatt agctgaaaaa
      541 atgcaggcag agattgctga aaaaatccaa aaattgaaag ataatggtat ccatcccgga
      601 ttagtcgttt tattggtagg ggaaaatcca gccagccaaa tctatgtaag aaataaagaa
      661 cgtgtagcaa aagaaatcgg aatccattca ttggtagagc gttatcctga gacgatttct
      721 gaagacgaac tacttgcaga aattgaaaaa tacaatcaag atcctagttt tcatggtata
      781 ttggtccaat taccattgcc aaagcatata gatgaagaaa agatcctgtt agctatccgc
      841 cctgaaaaag acgtagacgg ttttcatccg atgaatttag gcagactcct tgcgggtaaa
      901 ccggacaaga ttccttgtac tccatacggc atcatgaaaa tgtttgaagc ttattctatc
      961 gatccggcgg gtaaacgagc agtagttatc ggacgaagca atatcgtcgg taaaccgatg
     1021 gctcagctac tcttgatggc agatgcaact gttactgttg ctcattctaa aaccgaaaat
     1081 ctggctgaac tggcacaaga agcagatatt ttagtcgtag caataggacg tggacatttt
     1141 gtcacaaaag aattcgtaaa accaggtgct gtagtgatag atgtagggat gaatcgtgat
     1201 gctaacggta agctgatcgg tgatgtgaaa tttgatgaag tggagcctat tgcaggctgg
     1261 atcactcctg tgccaaaggg tgtagggcca atgactatca cgatgctgat gtatcaaacc
     1321 gtaaaatctg ccgaagcttt tggagcaggt gaatagatgg cacaagaata tttaactgtc
     1381 actacattaa cgaagtattt gaaaagaaaa tttgatgccg atccttattt agaacgcgta
     1441 tatttaacag gcgaaatatc gaatttccgt ttacgtgcca atgcccatca atatttcagc
     1501 ttaaaagatg accatgcaaa aatatctgct attatgttca aaggagcatt ccaaaaatta
     1561 cgattccagc caaaagaagg aatgaaagtc cttgtcgttg ggcgtatttc attatatgaa
     1621 gcgggaggtt cttatcaaat ctatgtagag cacatggagc ctgatggaat aggtgcttta
     1681 tatcaagaat tggaagaacg aaaagaaaag ctgatgaagg aaggcctttt tcaagggcct
     1741 aaaaaaatgc tgccacgttt cccaaagcga atcgcggtcc ttaccagtcc aagcggagcg
     1801 gttatccgcg atatcatcac gacggtgaaa agaagatatc caattgtcca attggttttg
     1861 ttcccaacag ttgtacaagg aaaccaagca gcggacaatg ttgtcaggaa tatccagaga
     1921 gtagaagaga tgggcaattt cgatacaatg atcatcggtc gtgggggtgg ttcgattgag
     1981 gatttatggc cgtttaatga agagcgggtt gctcgggcga tttatcaagc taatacgccg
     2041 gtgatttcat ctgtaggaca tgaaacagat accacgatcg ctgatttagt agcagatgtt
     2101 cgggctgcca ctccaacagc agctgcagaa ttagctgttc ctgttttgtc agaagagttg
     2161 atgcgcattc aagaacggca agctagattg cagcaggcgt ttgtacgcca gatccagcgc
     2221 aaacaagaac ggttcgagcg tgctcgacgt tcctatgtat ttcggcagcc tgaaagattg
     2281 tatgaaggtc agaccatcaa attagaccag ttgaaacagc gattatatca agctacacag
     2341 caaatttatc atgcgaaaga aaaacaaacg atcgcattaa gccaccaatt ggaacaagtg
     2401 gctccgcttt atcgagttaa agcagctcgc caagaaactg cttatctaga aaaacgctta
     2461 gaagagaaaa tccaacaata tatgaaacaa cagcagcagc gttttgctca agctgttcaa
     2521 tctcttgatt tgttgagtcc attgaaaatc atgggaagag gctacagcta tacgacgtta
     2581 aatgatcaag tagtaaaaag tgtcaccgag attcatcaag gggatttgct gacgatccat
     2641 tatcaagatg gaatcgttaa aggacaagta caagagatag acaaaggaga ataaaaatat
     2701 ggaaaatcca acttttgaag agtcactgca agaattagaa aaaattgtca tgcagttaga
     2761 acaaggagac gttccattag aatcagctct agattcgttt aaacgaggaa tggaattaag
     2821 caaacattgc caagatacat tagctaaagc agaagagacc ttgacgaaaa tgatgacaga
     2881 atcaaatgaa gaagttgatt ttgacggaaa cgaggaaagc tgagatgaga ttaactgact
     2941 tttcagctat tcatctacct gctgtagaaa aagaaatctt gtctttttta gatgagcata
     3001 caacggatag aggactctat gatgcgatga gttattcagt gaaagcaggc gggaaaagaa
     3061 tacgtccctt attactttta acggccgttg cttcctttga tgagcctatt gatgttcccg
     3121 tctatcaagt agcagctgct ttagaaatgg tgcatacgta ttccttgatc cacgatgatt
     3181 tgcctgcgat ggacaatgat gatcttcgta gaggaaaacc taccaatcat aaagtattcg
     3241 gcgaagcatt agctatattg gccggagatg gtttgcttac aggagcattc cagctaatca
     3301 gtatggcaca cttaggtaat tctcctaagc ttctgctgct tcagcaatta gctgtttgtg
     3361 cgggatcaca aggtatggtt gctgggcaag ctgcagatat cgagggcgaa tcgaaaaagc
     3421 tttctttaga agaattagca tttattcatg aaagaaagac agggcatttg attcgttatg
     3481 ccttgctggc tggtggcata ttagcgaagc aacctgaaga aatcttattg ctacttcaac
     3541 gattagccga acatttaggt ttagcttttc agataagaga tgatctactc gatgtgattg
     3601 gtacaacgaa aacattaggg aaaacagctg gtaaagatga acgtatggaa aaaaatactt
     3661 acccgcgttt attaggccta gaaaagacac gtgaggcgtt ggagattgag ctgttatccg
     3721 ccaacaagat catagacaaa ttagaagagg acgttttttc ttttgatggc gggctgatgc
     3781 gccagatgat caagcaattt gatgtggaga aatgagggaa agaatggaaa aagaacgcgt
     3841 agatgtatta gcggtcaaac aaggattatt tgaaacaaga gaacaagcca aacgttcagt
     3901 gatggctggt ttgatttaca acgaaaaaaa cgagcggttt gataaaccgg gagaaaaaat
     3961 tccagtgagt agtgaattaa gagtcaaagg taaaaaactt ccttatgttt ctcgaggggg
     4021 attaaaatta gaaaaagcac tcaaagcatt tgatctatct gttgaaggaa aaacattgtt
     4081 agatattggt gcatccactg gcggatttac tgatgcagct ttgcaaaatg gcgcaaaaat
     4141 gagttatgct ttagacgttg gctacaatca gctagcttgg aaaataagac aagatcctag
     4201 agtcgtcgta atggaaagag tgaattttcg ttatgcaaaa cccgaagact ttaccttagg
     4261 aattccagaa gttgctgtta tcgatgtttc ttttatttca ctaaaactga tgctgcctcc
     4321 attacatgct attttaaaag aagagggaga agtgattgct ttaatcaaac cccaattcga
     4381 agcaggcaga gaagctgtag ggaaaaacgg aattgttcgt gaccctcaga cccatcaaaa
     4441 agttttagaa gacattttgt catttgcggc acaaggtgga tatgatgtgc tggaattaag
     4501 ttattctccg atcacggggg gagaaggaaa tatcgagttc ttggctcact tgaggaaagt
     4561 acccgaaagc ggaacaatca attcagctat taatatggca gaagtggttt caaatgcaca
     4621 cgaacagttt gaccataaat aaattgtaac tgtgaaaatc aagtgagaag tatgcggatt
     4681 cttctttatt ataccaagga aataagctat cattgattaa gaagaatatg ctaaggagcg
     4741 aagacgtaat gagaaaaaaa gatcggcatc gtttgattac tcgattgttg agtgagcaag
     4801 atatccgaaa gcaggaagaa tttgttgaaa tcctgaaaag caaaggaata tctgttactc
     4861 aagctacgat ttccagagat ataaaggaac tgaaactgat caaggtaccg gcttcagatg
     4921 gcggctaccg ttatagcata ccagctgaaa caaacgaaga tgtaaatact aaattggaaa
     4981 aactattaaa agacgctttc gtggcagttg accaaatgga aaaatttgtt attttgaaaa
     5041 cattgccggg aaatgcttcg gcagcagcaa atttgattga taaacgttac aagaaagaat
     5101 tattttcgat tatcaatgat gacgataatg ttttgatgat tacacgaact gaagaccagg
     5161 cagttttatt gaagaaagac ttcctgcatt acctttaagt tgaggagcga atgtgatgct
     5221 acaagaaatc agtattacta actttgcgat tattccggaa ttgcggcttt cttttcacga
     5281 aggaatgacc gcgctgaccg gagagactgg tgcgggtaaa tccattatta tcgatgcttt
     5341 aggtctgtta gcaggaggaa gaggttcaag cgattatata cgtcaaggag cagaaaaatg
     5401 tgtgcttgaa gggttgtttg aactaccaaa acaagaagga ttctctgaac tgatggttga
     5461 attagggatc gaaacagatg aggacaatct gattgttcga cgggatatgt ccttaactgg
     5521 aaaaaatgtt tgtcgagtga atggtcatat tatcacatta gctaatctga gaagaatcgg
     5581 cagttattta gtagatatcc aaggacaaaa cgaacatcag gaactgctcc agcctgaatc
     5641 acatcttgct ttattggatc gttttggaga tgtagctttc caacagaaga aaaagagtta
     5701 tcagcaagaa tatatctctt atcgtgagct ggaaaaaagg gtacgaaaag tgcagcaaaa
     5761 tgaaaaatcc tatgttcaga gaatagatat gctccatttc cagcaagaag aaatagctgc
     5821 tgctgatttg caagtcggag aagaagagaa attaaaagaa gaaagagaaa aacttagcaa
     5881 ttatcaaaaa attgctgatg gattagctgc aggttacgga gcactgacga ataatgaaca
     5941 aaacagtgta gatggtgttg gcttggccgt ttcagaactg caagggatcg cccatctaga
     6001 tgtggagtat gaagcaattt atgaaaatat ccaaagtgct tactatttgc tgcaagatgc
     6061 aatcggcgat atgagtcgtc agattgattt attggagtta gatgagagtc gtttagaaga
     6121 agttacgcaa cgtttagaat taatccgaca gttgaaacgt aaatacggcg aatccattga
     6181 gtcaatattg gcttactatg acgaaatcac cgaagagcta gcttcttctg atttttcaga
     6241 aggtcaattg gacaaaatga aagaggaact agagcaaaaa gagcttctac ttcagcaaca
     6301 agccgcagat cttcatgaag cgcgtaaaaa aatcgcaaaa gaattggaaa aatccattct
     6361 gcatgaattg aaaagtctgt atatggaaaa cactgagttt gaggtacgtt tcttaaaaga
     6421 agaaaataga caattgaatc atgatggatt tgaccaaatc gaattttaca ttacgaccaa
     6481 tccaggagag ccactgaagc ctttggtgaa agtcgcttct ggtggcgaac tgtctcggat
     6541 gttgcttgct ttgaaaacta ttttttcttc tgagcaaggt gtgacaagta ttatttttga
     6601 tgaagtggat acaggtgtga gcggaagagt tgcacaggcc atcgcagata aaatttctca
     6661 aatatcgaaa tactctcaag ttttgtgcat cactcatctg cctcaagttg ctgcagtcgc
     6721 cgactatcaa tattatattg tgaaagcagt gattgatgga cgtacgcaaa cttctgtttc
     6781 agagttgaaa acaaaagaaa gagaagaaga aattgcacgt atgctggctg gaagtgagat
     6841 tacaaaactc acactcgaac atgcaaaaga actattgaaa ctcgcaaaaa ggacctgagc
     6901 cacctcgcag tggttcaggt ccttttcagc agctgatagc tatattttat ttttacatga
     6961 cactcattac agcagtcaat acaacagatc caatcgttaa aatcagcatt gtccaaatga
     7021 caaattttgt tactttagca aaagtacttg tttgtttttt ttcacgcatt gttttcaacc
     7081 tcgtcattaa ttagttgatt caagtgtaca atcttttttc catttccaca agagggcttt
     7141 tttataaaaa cttccctttt tttaaataat aggtagttac gatacaaata aataatgtta
     7201 atagaactag ccatataaag gcatcatccc tttcagcaat aggtaactta atgttcattc
     7261 catagatact gccgacgatt gtcggtatag ttaaaataat cgttaaagaa gttaaaattt
     7321 tcataatatt attcagatta ttcgatacga ttgctgaaaa agtctgattc atttgttcta
     7381 ctagttttaa ttgaatgcga gaagtagtca ttgcctgctc tgtttccacc aatacatcac
     7441 gtaaatgatt tttcaaagca tgattttctt ggaaaagttt attttgcgct agttctttca
     7501 atgcatctaa attagcagaa gtcgcttctc tgaaataaac gaggcttttt tggatatcca
     7561 tgatttgata aagttgtttg ttttcagtgg atacttgaag tttggcttcc aaagtatcgc
     7621 attgagattg aagcgtagct aaataactgt tgtaagagag tgtgacctgc caaatgaatt
     7681 gaaagaagat attgagatta ggcgaaatat cactttttgg aaaggtctgg ctaaagactt
     7741 tctttaaaaa cagtggttca tgattcgtaa ttgtgataag cttctttttg ggcgtcaaaa
     7801 taatagagag cgggtatgta tccacttgaa aataaccgct aggacttgtt ttagcaaaag
     7861 gatattgtaa caataataac gctggctttg tcaactttga ttgattgaat tcttctgtac
     7921 gggaattttc tgcatcgtcc aatatggaag tgatgtaatc tgacggaatt tcaaattgat
     7981 gtgtcagctg atgaatctct tcttgattag gacgttctac acaataccag atcgtttggt
     8041 cagtttcctt tccggtatta cgcagtttct cattatctaa tgtaaaatat ttgatcatga
     8101 ttttcctcct gttgcctgtt tcttttatta taacggatag ggaattggaa tacatggaat
     8161 gtgtttgtaa gatatagaat ataactgcat aaatttaaat aaaatgaaac agttgtgtaa
     8221 agtatataca gtatacttga agcaacgata aaaaaggagt ttacgttcat gggcacattc
     8281 acaccactta tcttgttttg gtatatttac ccggtaatcg ttttgtttgg ttgccaattt
     8341 cttgtaaaat tattttcgtt aaatcgtcgt tttaaaataa aagcccctga tttggcgatt
     8401 ccttttttgt ggggcggtat gcatgcattg tctagaaata cagggacaat ttctatctta
     8461 ccgtttttct ttatttctgt tttgctaatg ggaatttgta ttgctgtttt ccaagcatat
     8521 tattatgaag aaattattta tccgcgttat ttcaagatga cttggcgttt ggtttttctg
     8581 ttgacaatcg tcttgtacgc tgtcttgatc attgtgaaca tactttctta cctatgaaaa
     8641 aaatagaaaa gctgtaaaaa gtcttttaaa aggctttttg cagcttgatt tttaaaaaaa
     8701 tgtttatgaa taaaaaaagt tgttcccgga tttcccagaa aaggtggtag aaagtggggg
     8761 attgtggtag actaatttta gttagtggag gaatgggggg ctttcaatgt tgatgggtga
     8821 attccaacat aatattgatg caaaaggacg tttaatcgtg ccctcaaaac ttcgtgaaga
     8881 acttggcgaa aagtttgttc tgacacgcgg attggatggc tgtcttttcg gctatccgat
     8941 gagcgaatgg gaaaatctgg aagctaaact gaacgaaatg ccgttggcta aaaaagacgc
     9001 acgtactttt gtgcgattct tctattcggc tgccactgaa tgcgaacttg ataaacaagg
     9061 acgaatcaat atacccagca cgttaagaaa ttatgcagct ttgacaaaag gttgcgtagt
     9121 catcggtgta tctaaccgaa ttgaaatttg ggatgagaca cgatggcaag atttttctgc
     9181 agcagcagaa gagaactttg acgaaattgc tgaaagtatg attgatttcg gactataaga
     9241 ataggaagag gctgactaat gactgaaaca tttcaacact atacagtcat gttgaaagaa
     9301 acggtagatg gattgaacat caaaccggat ggcgtttatg tagactgtac actcggcggt
     9361 gccggacata gtgaatattt attaagccaa ctaagttcta aagggcatct gtatgcgttt
     9421 gaccaagatc aaaaagcaat cgatcacgcc aagctgcgtt tagcttctta tgtcgaacaa
     9481 ggtatggtca catttatcaa agcgaacttc agagaattag aagaacgctt gagtgagcat
     9541 gtagataagg tagacggcat tttgtatgat ctaggggttt cctctcctca gctagatgaa
     9601 gcagaacgtg gatttagtta tcatcaagat gcaccgttgg atatgcgcat ggatcagtca
     9661 gctcctttat ccgcttatca cgtaattaat gaatattcct accatgaatt agtaaagatt
     9721 ttcttccgct atggcgagga gaaattttcc aaacaaattg ctcgtgaaat cgaacgagta
     9781 cgaaaaacaa aaccaatcca aacaactggt gagcttgtcg aaatcattaa acaagtcatc
     9841 ccagctcccg caagaagaaa aggcgggcat cctgcaaaaa gaatttttca agctgtgcgg
     9901 attgcagtaa atgacgaact cggtgccgaa gaggcctctt tagaacaggc aatcgggtta
     9961 ttgaaagtca atggaagaat cagcgtaatc acattccatt cattggaaga cagaattgtc
    10021 aaaagtatgt ttaaagaata tagtacggta caagatctac caccgggact tcctgtcata
    10081 cctgacgaat tccaaccaga actgaaagtc attactcgta aaccaatcct tccttcagag
    10141 gaagagctaa cagaaaacaa tcgatcacgc agtgcaaagt tacgtattgc agaaaaaata
    10201 agaatgaaag aggagtagaa tgatggcaga attaaagaaa atgcaagatt acgaatatga
    10261 tttacctgaa ttggtggatc agccggaaca acaaatgcct gattcttctt cgaatcaaga
    10321 aatcattctt ccacaatcac cggcaaaacg attaaagcag atttctgctt tggaaaaaat
    10381 tgctgtggca agcgtggcga ttgctattgt tgctttatgt gttttaacag ttatgctgcg
    10441 cacaaatatc agcggagctg aaaaagaaat cacgtcaata caaacagaaa tcaatgataa
    10501 aaagcaagaa aaaacgagtt tagaacaaga aaaaaacgaa ctttctcgta ctgaacggat
    10561 taaacaaatc gcagaagaaa aaggattgtc tattaatgac gacaatttaa ggaaagtgaa
    10621 gtaaatgagt cttaaaaata aaattcggcg gtttatggaa aagaaaaatc taaatccgat
    10681 gaacaatcgc aaaaaagtag gtatcatttt atttgctacg agtattggat tattcttttt
    10741 atttgccgtc cgtttctcgt atatcgttat tggaggtcat gttgctggta cctcattagc
    10801 tgaaaaaaca aaacagttgt accaaggaag tgaagtagta aaagcaaaaa gaggaacgat
    10861 ttatgatcgt aacggtgtgg cgctcgcaga agatgcctcc tcttattcaa ttaaagcgat
    10921 actctcaaaa acatatacct ctggggataa aaaactgtat gttgaagaaa agaattttga
    10981 taaaatagca gaaatcctgc ataagaatct ttctatagac aaaaaagatg cactgaatat
    11041 tttagaggac ggcgcaaaaa aagagctgta tcaagttgaa tttggtagct atgggaaaaa
    11101 tatctcccaa gaaaccaagc aaaacatcga agcggacatg aaaaaagaag gtgtcgctgg
    11161 tctttatttt gtcgaccatc aggctcggat gtatccaaat ggcgtctttt cttctcattt
    11221 tattggatac gcagttccag acaaagatga gaatggctta gttggtaaac taggtttgga
    11281 gtctgcttac aatgatatct tgagcggtaa agatggtaag attatttatc aaaaagataa
    11341 ttttcaaaat cctctgccag gtacagtggc agaagaagaa aaagctgtcg acggacaaga
    11401 tatctacacg actttagaca gcagactaca aagttattta gaaacactga tggatcaagt
    11461 aaatgaagaa tatcagccag aagaattgac agcagtcctg atgaaagcaa aaaccggaga
    11521 aatcttggca atgggacaac gacctacttt caatcctgaa acaatggaag gattgactgg
    11581 aaaagatgct atctggcgta atttcttggt acaagatagt tatgaaccag gatcaaccat
    11641 gaaagttttc actactgcag ctgctatcga agaaggagaa ttcaacgaga atgaaacctt
    11701 ccaatctgga aaaatccaag tagaagatgc tacgatcaat gaccatgact ttggtgaaaa
    11761 aggtgtgctg accatgcgtc aagctctttc atggtcaagt aacgtcggga tggtcatact
    11821 ggaacagcga ttaggcggac gatggtataa ctatttacaa aaactgggat ttggacaaag
    11881 tactcattct ggattagatg atgaagtaaa cggtgcttta cctacattaa atattgtcga
    11941 ccgagcaatg agtgcttatg gacaagcggt aggtgtgacg aatttccaaa tgatgaaagc
    12001 tttcacttct attgcgaata acggtacaat gattcaacct cgatatatca gcaaagtggt
    12061 ggatccccaa actggagaag agcgaacaac tcaaacagaa gttctagggc aacctttttc
    12121 aaaagaaaca acagaaaaag tccgtgaata tatgagagat gttgtcgaaa gcgaaaacta
    12181 cggtagtgct tatggtgtat atagtgtacc tggatacaat gtatcggcaa aaaccggaac
    12241 agcccaaatc gcttctgata cagggggata tcaaacagga gatacagcct acctgtattc
    12301 gattgtggag atggttcctt ctgaagatcc agactatgtc ttgtatctaa caatgaaaca
    12361 ccctaagaca tatgatcgaa tggcattggc taaaatcgca aatccattga tgaaacgggc
    12421 aatggatttt aaagaaactg aagaagattc agatacagaa acaaaaacag aaaaagtatc
    12481 cgtagccgat tatcgtaatc ttgaagcgga cgttgcagca gctgatgctc aaaaaagtgg
    12541 actgcaacct gtagtgatcg gtaatggtaa aaaagtacaa aaacaatcta ctgccaacgg
    12601 tgatcagttg atctctggag aaaaactgat cctatatact ggtggtgata agctgatgcc
    12661 tgacgtaaca ggatggtcaa aagcagatat catgaagctg gggaaaatcc tcggtattga
    12721 agttagtttt gatggagatg gttattgtgt caaacaagaa cttgctccat atgaaaaaat
    12781 cactgaagac aaacttagtt ttactttaga agaataagaa gagaaatagg agagaaaaga
    12841 gcatggaatg gacacaagca ttgattccca ttgtcagcag ttgtgcaatg acaattgccg
    12901 caatgccgct atttattggt tattttcaaa tgaaaaagca aggtcaggcg attcgtgagg
    12961 agggaccaaa gtggcacaat agcaaagccg gaactcctac aatgggcggc ttagtgtttc
    13021 tgatcggctc tattttgaca ggaatttggg ttggagcttg gcaaaaacag ctaactccta
    13081 ctcttttcat cttgcttttt gtattagcgt tatacggagt aatcggcttt cttgatgatt
    13141 ttataaaaat attcaagaaa agaaacatgg gactgaactc gaagcagaaa ttgattggac
    13201 agattctagg gggaatcatc ttttatcttg tttaccgttc tgaaggttat ccaggtgtat
    13261 tgaatttctt cggactagat ctacctttag gaatcattta tggtattttc gctctcttct
    13321 ggcttgttgg attttcaaat gcagtaaatc tgacagatgg catcgatgga ttagttgctg
    13381 gcttaggaag tatctctttt gcagcttacg gtctaattgc ttggcatcag catcagtatg
    13441 atgtattaat catttgctta agtgttttag gaggtttact gggtttcttc gtctacaaca
    13501 gaaaacctgc caagattttt atgggagatg tcggttcatt agcgttgggc ggacttttag
    13561 cggcaatttc tataatgcta aaccaagaat ggacattgct tttgattggt ttgatctatg
    13621 ttatggaaac agcaagtgtg atgcttcaag ttacttcctt taagcttaca ggaaaacgaa
    13681 tttttaaaat gtctccaatt catcatcact ttgaaatgtg cggatggtcg gagtggaaga
    13741 ttgatacagt tttttggttg atcagcattg tgacttcgtt gatcacatta tggatcgttt
    13801 ggtaggagat gacgggaaat gaaaaaaata tcaacatatg aaaataagaa agtgcttgtc
    13861 ttaggactcg caaaaagcgg agtaagtgcg gccaaattat tacatgaatt gggcgcactt
    13921 gtcaccgtta atgatggaaa accttttgac gaaaatccgg aagcacaaga gctgctttct
    13981 ttagggataa aagtaataac aggtagtcat ccaatcgaat tgttggatga agaattttca
    14041 ttgatggtca aaaatccagg gattccctac agtcatcctt tagtagcaaa agcccaagaa
    14101 atgggtattc cggtaattac ggaagttgag ctagcttacg aagtagcgga atgcccaatt
    14161 attgggatta caggaacgaa cggaaaaaca acgacaacaa cgatgacagg actactactg
    14221 aatgcaggag cagatcaagg aattgcacgc ttagcgggta atattgggta tccagcaagt
    14281 ggtgtagcgc aagaagcaaa atcagaagat aaaattgtga tggaattgtc tagcttccaa
    14341 ctcatgggaa tcacagattt ccgccctcat atagcggtga ttaccaatat ctatgaagca
    14401 catattgatt atcatggtac aagaaaagaa tatgtcaaag ctaaatggaa ccttcaaaaa
    14461 aacatgacag aaaaagatta tctgatcctt aactggaatc agtcagaact acaagaatta
    14521 gcgcaaagaa caaaagcacg cgtactgcct ttctcgacaa aagaagtgtt ggaagacggt
    14581 gtgtatgctg acgattactc aatatactat aaaaaagaaa aaataatgga aatatctgaa
    14641 cttggcgtac caggtaagca taatgtagaa aatgcactag ctgctatttc tgtagcaaaa
    14701 ttgtatggta tatcaaatga agctatacga gaaacgttgc attttttcca tggcgtacca
    14761 catcgtacgc aatatgtagg agaaatccaa ggcagaaaat tttataatga ttcaaaagca
    14821 accaatattt tagcgactaa aatggctcta agtggatttg aaacgtctaa ggtcgtgtta
    14881 ttggctggcg gattagacag aggaaataca tttgatgaat taattccttc attaaaagga
    14941 atcaaagcaa tggtcgtatt tggccaaaca aaagaaaagt tgatggatgc tggaaaaaaa
    15001 gcaggcatcg agacaatagt aacagccgat tctgtagaac aagcagttcc tttagcatta
    15061 gaaaacagta cagatggtga tgtagtttta ttatcaccag cgaatgccag ctgggatcaa
    15121 tacccaaatt ttgaaactag aggaaacaga ttcatggaag ctgtgaaccg attaaaatag
    15181 aaaaagtaga agcgagcgat aaaaaatgaa aattttggtt acaggcggag gaacaggggg
    15241 acatatttat ccagctcttg cctttgtcaa ttatgtaaag actcaagaac cgaatgctga
    15301 attcatgtat gtgggagcaa aacgaggtct agaaaacaaa atcgtccctg atacaggaat
    15361 gcctttccat accttggaga ttcaaggatt taaacgaaaa atttccatgc ataacatcaa
    15421 gacggtccga ttgtttctta agagtatccg tcaagcaaaa aaaatcctca gtgattttca
    15481 accagatatc gttatcggta ctggaggata tgtttccgga gcggtagtgt acgctgcagc
    15541 caaaatgggt attccaacag ttatccatga acaaaacagt gtaccaggtg ttaccaataa
    15601 atttttaagc cgatatgtgg atcggatcgc tctttcattt aaagatgcgg cttccttttt
    15661 tcctgaaaac aaagcagttc ttgtaggtaa tcctcgagca caagaagtag cagatactaa
    15721 aaaatcagat attttgaaaa catttgattt ggatccagaa aagaaaacag tcttgatttt
    15781 tggcggtagt caaggagcac ttaaaatcaa tcaagcagta tcagtttttt taagtgaata
    15841 tattccttct tcttaccaag ttttgtatgc ttcaggagat agatattatg aagagatcct
    15901 aaataaaata aatcctgaaa tacaaaatat tagtgtccag ccttatatca aaaatatggc
    15961 agaggtcatg gcttccagtg atctgctggt ggggcgtgct ggtgcaactt cgatcgcaga
    16021 attcacagct ctggggttac cggcagtgtt ggttcccagc ccatatgtta cgaatgatca
    16081 tcagactaaa aatgcaatga gtcttgttca cgcaggagca gcaaaaatga ttgcagataa
    16141 tgaactaaca ggtgaatcac taagtcagac agtcaacgaa atcatgggtg atgaagaatt
    16201 gcaaaagcaa atgtgccgag cttctaaaga acaaggaatc cctgatgctt caaaacgtct
    16261 ttatgactta gtaaaacaaa taatcaaaaa ataaaggcgg tggtcttagt gagtaagaaa
    16321 aaagaaccaa aaaaattgtc tcatccgtct tctgatgaaa acaatttgac tccttggcaa
    16381 aaggctaacc gaaaatatct ggcagaacaa gagatgaaaa accaagagcc aaaaaaagaa
    16441 gacataacat tagatacacc cctttcagat tctgaagaag aaaaaacggt tcaagaggaa
    16501 cagcaagaag aaagccagtc cgagaaaaaa atcgaatttt ttgaagagat caagcaaaag
    16561 aaaaacggtg gaccatataa cggttctttt ttgaaccggc tgcccaattt aaaaagtcaa
    16621 cggcacaaag tactatttcg tcggttagct ttgatcatca ctgtattggg tataccgctg
    16681 atttttttga tctattatgt ttctccttat agcaaattac aagcagttgc agtatcaggc
    16741 aataagacag tcaacagcca agaaatcatc tctgatacaa aattgtcatt aggagaaaat
    16801 gtttggggac agtattttca tcgatctact tatatcgatc gccttaaaaa agcacagcca
    16861 agaatcgaaa cagcaaatat acatttcaaa gggatgaatg aattcgattt ggatgtaaca
    16921 gaatataaag agattgcttt gatcgcaaaa aacaatcaat actacccagt gattgaaaat
    16981 ggaacagtat tagatgaaaa agtagcaaat cctacaaaaa atctccctat cttggaagag
    17041 ttcaaagact ctgcaaaaat caaagaatta acaaagcaat acaatcagtt atcatcagaa
    17101 ttacaaaaag caatttctga aatcaagtac acgccaagag caagcaataa aaatctgatc
    17161 caattgaata tgaatgacgg caatcaagtt attgtaaata tcaataatct tgcgaaccaa
    17221 atgaaatatt attcgcaagt agccaaagat atggatgaaa aaggtgtgat cgacatggaa
    17281 gttggtatct tctcgcatcc ttacggtagc actcaagaaa cggaagaatc gggccaatcg
    17341 gaagaaacaa ctgaaagtga agaaaatgcg gatcaaacaa acgaatcaag tgaagaggca
    17401 gaaacgcaaa aagcacaaac acctggatcg tctagcacaa acggatcatc tgattcaaat
    17461 gattcaagtg aaacaagtca aatagaagat aatccaagta caactccgtc aagtttaaat
    17521 gaaaattgat aaaacaactt taaaaagtaa gaatgtactt tctaaaaacc agtaatttat
    17581 ggtagaattg gaagcagtga atatatctat taataagtat attttaaaac ggacgctagt
    17641 tgttcgcttg tatatggaat agtaagtagg agggaatccc attcatggca aaaacaggaa
    17701 tgtatgtagg ccttgatatt ggaacgacat ctgttaaagt tgtcgtggct gaatatatcg
    17761 acagccaaat gaatattatt ggagtaggaa acgcaaaatc agaagggatt aaccgaggca
    17821 ttatcgttga tatcgataaa acggtccaag cgatacaacg agcagtacga caagcagaag
    17881 aaaaagcagg tatccagatc aaaggcgtaa gtgtcggttt gccagcaaac atgcttgaag
    17941 tagaaaattg ccagggaatg attgcagtta acggagattc aaaggaaatc accgatgaag
    18001 atgtcagaaa tgtggcttca gctgcccttg ttcgttcaat acctcctgaa cgtcaaatcg
    18061 tgtcaatatt gccacaagat tttactgtcg atgggttcga gggaatcaaa gatcctcgtg
    18121 gaatgatcgg cgtaagatta gaaatgtacg gtctgttgtt tacaggacct aaaacgatca
    18181 tccataacat acgtaaatgc gtggaaaatg ccggattaat cgtcaacgaa atggtgatta
    18241 ctcctttagc gctgactgaa tcaattcttt ctgacggaga aaaagatttt ggtacgatcg
    18301 tcattgatat gggtggcggc caaacaacaa cggctgttat gcatgataag caattgaagt
    18361 ttaccaatct tgaccaagaa ggtggcgaat ttgtcactaa agatatctct attgtattaa
    18421 atacgtcatt taacaatgca gaagcgttga aaataaacta cggggatgca tacccagaac
    18481 ggacatcacc agatgaagaa tttccagtag atgtgattgg tcagtcagaa ccagtgaaag
    18541 tcgatgaacg ctacttgtca gaaattattt cagctcgaat ggaacagatt ttcaacaaag
    18601 caaaagatgc attggaccaa atcgaagcat tagagttgcc aggtggcgtc gtattgacag
    18661 gcggagctgc tagtcttcct ggtgttgtgg acttggcaca agaaatcttc ggcgtaaatg
    18721 taaaattgta tgttccaaat cacatggggc tgagaaaccc ggtatttaca aatgtaataa
    18781 gtatcgtgga ttattcagca aacttgagtg aagtgtatca attggcaaaa attgctgtta
    18841 cgggtgaaac ctcagcagct cgtcaaatgg tagttgaaca agaaacaaca aacacatatg
    18901 agagctatga agcacctgaa gaaacaatct acgatgagcc ggaaccaaaa gagtccggag
    18961 aaaatgtaaa aaataaaatc aagggtttct ttacaaatat ttttgattaa ttcatcctgg
    19021 gaggaataag atatggaatt ctcaattgat aataatatca atgacggcgc agtaatcaaa
    19081 gtcatcggag tcggtggtgg cggtggaaat gctgtaaacc gaatgattga agaaaacgtt
    19141 aaaggtgttg aattcattac agccaacaca gacgtgcaag cattaaaaaa ttcaaaagct
    19201 gaaacagtta tccaattagg ccccaaatat actcgtggtt taggtgctgg ttctcagcca
    19261 gaagttggac aaaaagcggc agaagaaagt gaacaatcct tacgcgaagc tttagacggt
    19321 gcagatatga ttttcatcac tgcaggaatg ggtggaggaa ccggtacagg tgctgcacca
    19381 atcgttgcag gtatcgcgaa agaattgggc gcattgactg ttggtgtcgt gacacgtcca
    19441 ttcacttttg aaggaccgaa acgtggccgt tttgctgcag aaggtatcgc tcgtttgaaa
    19501 gaaaatgtgg atacattatt aattatctca aataaccgtt tattagaagt tgtagataaa
    19561 aagacaccga tgcttgaagc tttccgtgaa gcagataatg ttttacgaca aggtgtacaa
    19621 ggtatctctg atttgatcac agcgccaggt tacgttaacc ttgactttgc tgacgtgaaa
    19681 acagtgatgg aaaaccaagg tacagcattg atggggatcg gtgtggctag tggagaagat
    19741 cgcgtagtag aagcaacgaa aaaagcaatc tcctctccat tgcttgaaac atctattgat
    19801 ggagctgaac aagtacttct taacatcaca ggcggtttgg atatgacatt gttcgaagca
    19861 caagatgctt cagatatcgt agcaaatgca gcaactggcg atgtaaatat cattctaggt
    19921 acatctatca atgaagaaat gggcgatgaa atccgtgtga cggtcatcgc aacaggtatc
    19981 gatgaatcaa aaaaggaacg caagtctagc cgaccagcta gacaagcaca aatgcaatca
    20041 cctgctcaaa agactgtatt agatatggat caagctaaac caacttcttc agaagaagaa
    20101 aacagttttg gcgattggga tatcagacgt gaacaaaatg ttcgtcctag agttgatgat
    20161 tccaattttg acaatatcga aaaaaaagaa tttgatacat tcaaccgcga agaaacaaaa
    20221 tctcatggcg atgatgaatt aaatacgcca ccgtttttcc gtcgtaaaaa ataaaggaga
    20281 ggaagagcac atgattgctg acaacttgca gaaaatcaag caagaaattc aggattcgtg
    20341 tgctcttgtt catcgtgatc cgaaagaagt tacattagta gctgtaacaa aatctgttga
    20401 atccgctcaa gctgaagaat tggttagagc tggcgttatg aattgtgcag aaaaccgagt
    20461 agataaatta ttagaaaaga aacaagattt atttgatttt cctgaaataa agtggcattt
    20521 aattggtaat ttacagcgca gaaaggtaaa attgattgta aatgaaattg attactttca
    20581 tgcgttggac agtttaagat tagcagaaga aatccagaag cggttagaga aagaattatc
    20641 ctgttttatt gaagtaaatg tttctggtga ggaatcaaaa catggaattt caccagatga
    20701 gcttttaccg tttgtggaat ctttagctgc ctgcacaaaa atcaaggttg ttgggttgat
    20761 gactatggcg cctaaagaag cttctgatga gcaaatccat aaagtgtttg gtacgctgaa
    20821 agaattgcgt gatcttgttc aagcaaaagg ttatgcccat gcaccatgta gtgaattaag
    20881 tatggggatg agtcaagact ttcaaattgc gattgaagaa ggtgcaacat ttatccgtgt
    20941 aggaacagcg ctattcaaag acgaataaag gaggaacaaa aatgccgctt ttaaacagag
    21001 gagcaattgc taattttttc ggattagctg atgaggaaga gtacgaatac caagattatc
    21061 ctgaaacaac aaaaaaggct gtacagccaa ctaaaaaaaa tacaacacag aatcaatcaa
    21121 ctcattctgt atctgctgcg aatactcatg aaaaacaaaa aacggcgact tcacgtcctg
    21181 taacgcatga aagtattcag ccggctgaaa agaaaaaagt aattgaacta catcaatcac
    21241 ctacaaaaac tgtagcgaaa gaaagaacag cttcaagaac ctctacacaa cctaaagcaa
    21301 atcttgctgg aaaaatcact gtaatggaac caagagctta ttctgaagct atggcgattg
    21361 ctaaacgaat cattgctgga gaagcaacct tagtgaattt tcatttagta gaagaaaaac
    21421 aagcgagaag aatcgttgat tttctaacag gaacagttta tgcactagat ggagatatca
    21481 aacgtgttgg agatgagatt tttctttgta caccgactgg gatggagatt gacagttcaa
    21541 ccgctcaatc ttttgcagat gcgaatttgt ttgatctgta agaggaggaa ctacgattta
    21601 tactattgca ctattacttc atttacttca acgggcagcc tatttctata gcattatctt
    21661 gatggtatat gcattgctct cttggtttcc aggcgggtat caatcagggc taggacgatt
    21721 attagctaag atctgcgaac cgtatatcag tttgttcgat cgtctgcctt tgaggattgg
    21781 acccttggat ttcacaattt taggggcaat cctgatcttg aacttagccg tacaaggttt
    21841 agcacagatc gttatatgga taactgtcca tttagtttat tgattgaagc aacaaaatct
    21901 ttattctcag aatagatagg aggggaaaat tttggatgca aatgtatacc agcactttcg
    21961 ggcagatgaa cgtacattta tcgattcggt aggcgattgg attgaacagg ttcaaggaca
    22021 atatgcaccc tatctgaccg aatttcttga cccaagacaa tcctatattt tagaaacact
    22081 gattcgacaa agcagtgatt tgcgtttcat gttttatggc ggatatgaac aggcagaaag
    22141 aaaaagatgt ttgattttcc ccgattatta cgaacctgta gaggaagatt ttgaaacagc
    22201 attgtatgaa atccattatc ctagcaaatt tgccagccta agtcatggga aaatccttgg
    22261 aactttagtg ggaacgggga tcaaacgctc ttactttggc gatatcatct cggacggtga
    22321 tcgctggcaa gtatttattg caaatgaggt ggatcatttt gttgctgcac aagtaacaaa
    22381 gattggaaag gttgctgtac gcttggaacc aatcaaatac acagaaatga ttatgccgaa
    22441 agatagctgg acacaagaaa gaggaacagt tagttccatg cgactagaca gtgtgatctc
    22501 tgagatttat aatatttcca gacagcggtc caagcagctg atcgaagctg gtaaagtaaa
    22561 agtgaactgg acagaaaata caagacctga ttttgtactt gaactattag atattatttc
    22621 agtcagaggt tatggcagag tccagatcca agaacttgag gggaagacga aaaaagaaaa
    22681 gtacagggta ctgttcggtg tattacgtaa ataaaataaa aaggtggttt actcatggct
    22741 ttaactccat tagatatcca aaataagaca ttcccgacaa aaatgcgggg atataatcaa
    22801 gacgaagtag atgatttctt agatttagtt gttcgtgact atgaggaatt gactcaaaga
    22861 aatcgagaat tagaaaaagc agtcaaacat tctgaagaaa aacttgagta cttcaatgaa
    22921 ttaaaagatg ctttgaacca atcaatcatc gtagctcaag atacggctga taaagtaaaa
    22981 acaagtgcaa gcaaggaatc cgaagtgatc gttacttctg cacaaaacaa agcagatgag
    23041 ttagttgcga acgctgaaaa acgtgcacat caattaacaa ctgatgcaga agaaaaagca
    23101 agaaaaatct tgacagatgc aacagaaaaa gcaagacagc ttgcgacaga aactgaagat
    23161 cttaagaaaa aaacacgagt attccatcaa cgtatcagct tgatgcttga gtctcaatta
    23221 gaacaagtga aaagtccgga atgggatgaa attttacaac cattctccag ctacgtgact
    23281 gacagccatg aagtgatcaa agaagtttta gcaaaacagc ttgacaatga aaatgatact
    23341 gatgtaaact cagacacagg agtcatagaa gcacctatga cggcatttga tacacaggct
    23401 attgatatct cgatcgtgcc tggtgaaaat gaaaacgagt agaacagaaa agcactttgt
    23461 tattcttaaa gcgagccgat gagagtggaa gttcggtaag acactgcatg gtgaaagatc
    23521 ctctacttga gacctgcttt tccgaaatta gagtaagaag cagcggctga cctcgttatt
    23581 ggtcacagag agtttgcact agatagatgc aactaaattt gggtggtacc acggcatgtt
    23641 cgtcccttgc ggctgaacat gcttttttgt gtccggaaaa caaataaagg agtgtatggt
    23701 tgaatgaaaa tgaaagaaac cttgcaatta ggaaaaacaa aattccctat gcgtggtaat
    23761 ttgccaaacc gagaaggcga gtggcaaaaa gaatgggaag aaaatcgaat ctaccaaaaa
    23821 agacaagagt tgaacgaagg aaaacctagc tttgttcttc atgatggacc tccatatgcc
    23881 aatggtaata tccatttagg acattcattg aataaaatca gtaaagacat tatcattcgt
    23941 tcaaaatcaa tgtctggcta tcgtgcacca tatgttccag gatgggatac acatggattg
    24001 ccgattgaac aagtattggc taataaaggc gtgaaacgta aagaaatgtc catggcagaa
    24061 tacttggaaa aatgtcgtga atatgcactt tcccaagtag ataagcaacg agcagatttc
    24121 aaacgcctag gagttgcagg agattgggaa catccttatg ttacattaga tccttcttat
    24181 gaagctgcac aagtacgcgt atttggtaaa atggctgaaa aaggctatat ttacaaagga
    24241 ttgaaaccaa tttactggtc tccgtcaagt gagtcttctt tagcggaagc tgaaattgaa
    24301 tataaagatg taaaatctcc atctattttt gtagctttca atgtcaaaga cggcaaagat
    24361 attcttgatg aagatacagc ttttgtcatt tggacgacaa caccatggac attgccagca
    24421 aaccaaggta ttgctgtaaa tccaaaattt acgtatgtat tggtggaagc cgatggccgt
    24481 aagtttgtcg tagcgaaaga tttgctagaa acagtccaat cagaaatcgg ttgggaaaat
    24541 gtaacagttc taaaagaatt tgctggacaa gaaatggaat atatgactgc aagtcatcta
    24601 ttctatgaac gtgaatcact tgtgatttta ggcgatcatg ttactttaga cgctggtact
    24661 ggattagtcc atactgctcc aggacatggg gaagatgact atatcgctgg tagcaaatat
    24721 aaactatctg tagtgtcacc tgtagactca aaaggattgt ttacagaaga agcacctgga
    24781 tttgaaggta tcttttatga caaagcaaat cctatgatca ctgatctatt aaaagaaaaa
    24841 ggagctttgc tgaagctaga tttctttact catagttatc ctcacgattg gcggacgaaa
    24901 aagcctgtga tctatcgggc aactccacaa tggtttgcat ctatcgataa attccgtcag
    24961 aatattttgg atgaagtaga aaaagtagag tgggttatcc catggggtaa aacacgtttg
    25021 tataatatga tccgtgaccg cggcgattgg gtaatttccc gtcagcgtgc atggggagta
    25081 ccacttccga ttttctatgc tgaaaatgga gaagcaatca tcacaccaga aacaatcgaa
    25141 catgtggcaa aattatttgc tgaacatgga tcaattatct ggtttgaaaa agaagcgaaa
    25201 gaacttcttc ctgaaggatt tacccaccca ggttctccta atggcgaatt tacaaaagaa
    25261 aaagatatca tggatgtctg gtttgattct ggttcttctc atgaagctgt tttacgtcag
    25321 cgaccagaat tatcattccc agcagatatg tatttagaag gatcagacca atatcgtggc
    25381 tggttcaact caagtatcac gactagtgtg gctatcaatg gtgtagcacc atacaaatca
    25441 gttatttctc aaggatttgc tttagatggt gaaggccgta agatgagtaa atctttagga
    25501 aacatcattg caccagaaaa agtgatcaaa caatttggtg cagatatcct tcgcttatgg
    25561 gtatctagtg tagattatga agcagatgtt cgtgtttcta tggatatctt aagtcaagtg
    25621 gcagaagtat atcgtaaaat cagaaatacg atgcgtttct tattagcgaa tacagaagat
    25681 ttcgatccta aaaaagatac tgtttcgtac aatgatttac gttctgtaga caaatatatg
    25741 accgttcgat tgaaccaagt aatcaaagaa atccgagaag aagggtacga caaatataac
    25801 ttcatgcata tttaccgtac agttatgaac ttcttgacgg ttgatctatc gtcattctac
    25861 ctggattttg ctaaagatgt agtttatatt gaagcagaag atgattatca acgtcgttgc
    25921 atgcaaactg ttttctatca aacagctgta gctttgacaa aattattgac tcctatcatt
    25981 ccgcatacag cagaagaaat ctggtcattc ctgaaagaag aagaggagta cgttcagtta
    26041 agcgagtttc ctggttatga agagtttgca aaccaagaag aattgatgga tacctggact
    26101 gctttcatgg acttccgtga taatgtctta aaagcattgg aagaagcaag aaacagcaaa
    26161 ttgattggta aatctttgga agcaaaacta actgtttatc caaaacaaca agttcgtgaa
    26221 atgttaaaag ctttggatgc agatattgca caattattga tcgtttcacc agactacttt
    26281 gaagttatgg cagcagatga acaagtgcca gataacgcta tggtatttga tgatgttgct
    26341 attacagtgg aaaaagcaga cggggaaact tgcgatcgct gtcgccaagt tcgtaaagat
    26401 gtaggagtag atgaaaaact tcctcatcta tgcggtcgtt gtgcaaaaat cgttgaagac
    26461 ttctatcctg aagctgtagc tgaaggattc gaagaaaagt aaaagggaaa agggggtgtg
    26521 acatgttctt tgtcacaccc ccttttgtta tcaatcgttt aattttccac gttcacgata
    26581 ccagttatct ggttgccaga tccattcgaa tccgtctttt tcaagtagtt cgaaagctgc
    26641 tttaggtccc attgatccag ctttgtagtt gggaaaatcc ggttgtgttt ggtcccaagc
    26701 ttttcgaatg acatccacga tatgccaaga ttgagcgact tcttcccaat gagagaagtt
    26761 cgttccatca ccattcaacg catctaatag taatttttcg taagcttctg ggctattttc
    26821 aaccatttct gcactatttc taaattccaa tttgattggt tccgtttcaa atccttgccc
    26881 tacttctttc ccattcaagg ataaagaaaa tccttcagtt ggctgaatat agatcgttag
    26941 aacattaggt ggaagtgtcg tatcgtcaca tggttcatcg acagatgttt tgaatacatt
    27001 aacaggaact tgtttgaata cgatgttgat ccgagtccct ttttcagtca aacgtttgcc
    27061 tgtacgtaca tagaaaggaa cacctgacca acggaagtta tcgatagtga atttgcctgc
    27121 gacgaatgtt tcagtctctg attgagcatc cactttgtct tcttcgcgat agcccttgaa
    27181 ttgtacacca tccaattcac cttgagcata ttgcccgcga acgaagtttt ctaacacttc
    27241 ttcttcgtta taaatgcgga tagctttcaa tgctttgatc ttttctgtac ggatttcttt
    27301 ttcggaaaat gctactggcg gttccattgc aagtaatgct acaacctgta aaatatgatt
    27361 ttgcaccatg tctttaagtg cgccactatg atcgtagtag cccccgcgat cttctacacc
    27421 gaggttttcg ccaaaagtaa tttggatatt gtcgatatag cgattgttcc attgagcctc
    27481 aaaaatatta ttggcaaaac ggatagctga gatgttttgg atcatttctt ttcctaaata
    27541 atgatcaatc ctgaaaatat cttgttctgg gaatacttct ctgatttgct ggttcaattc
    27601 aaaagcagac tggtaatcag aaccgaacgg tttttctatg atcaaacgat tgaagccatt
    27661 ttttgtcata atattttgtg atttcaaatg agaaacgatc gttccaaaaa attgaggtgc
    27721 catagccaaa taatagacac ggttaccgtt taattgatag tgtccatcta atcggtcaga
    27781 caattctttt aatgtctgat aatgcgttga atcatttaca tcatgggatt gatagtaaaa
    27841 atggcttgca aaggtagttg cttcttcatc tgaaggattc aatgcacgga ttgtttcttt
    27901 gacaacttca cggtagtgct catcgctcca gggacggcga gcggttccga ttacagcaaa
    27961 atgctcgcct aaatttcctt ttctgtaaag gcggaacaaa gacgggtaca atttacgctg
    28021 ggctaggtca cctgtaccgc cgaatatcgt aaacaataca tttttttcat tagacatgtg
    28081 gatacactcc tttatatttt atcgacaaaa atcgtgctgg ccgccttaaa gctgacgggg
    28141 attttctttc tatcattttg gatagtgatc ggtccttcat aagctgcgat gtcaacgatc
    28201 tcatattctt cgttgatatt caggtcaata gtgactaagt aatccaacaa ttctttttca
    28261 tcaagcactc gtgcgatacg cacttttgta ccgacaggat aggactccaa tgtttgtcgg
    28321 cgttcttcat ggacaacttc ctctttttta ggaatcgatc ctccatgagg acaatgttca
    28381 ggatgattca aatagttttc taaatgatct gcaagatgtt ccgaagtcac atgttccaat
    28441 acttcagcat catcatggac ttcattccag ctatagtcta aatgttcaac taaaaaaact
    28501 tcccataagc gatgtttgcg aatcagactg ctagcttttt gcagtccgct tgcagtcaat
    28561 tgtacacctt gataagggga atgttctaca agctcttctt tcacaagttt agaaatcatt
    28621 tcactgacag atgcagctga gacatctaga ctagatacga tttgtttatt gttgactttc
    28681 gttgtatctc cacctagttc taaaatgatt ttcaaataat cttctcgatt tggtgtcata
    28741 ttcgatcatc ctttcatggt aaaaaaccat cattagtaag tgtaacaaaa aaaacgttaa
    28801 ttgactattt ttctaaataa aaaacaaagg atttttagac gtttttacaa caaaaaacga
    28861 aagatgatag agaagactca ttctttcgtt tataaaggat atatatatac attgggaggg
    28921 atctattttt cagataacaa cttatatgaa gtgtctgcag attcgtcata aagagtttgt
    28981 tcgggataag aaaagataag ataaaaacca tactgatcac gtacgactaa acgatattca
    29041 tctatttcta ccacagatgt gccaagagac tgaccgttga tcaaaaacgt gtaatcagat
    29101 gtaatctgta attgttgttt ttgctggcta tcactaaaga accatgtacc ttgatacttt
    29161 gagagtcctt ctgacttttc cgcttgtttt ttctttttca agtaaataaa acttgagaga
    29221 acggtaatga gtgaaccaag aaaatagatc atttttttta tcaccaaatc actcctttat
    29281 gagaaaataa tcgcatagac atattctttc ttctttattt cattgtaccg aataaaagct
    29341 aaaatttctc taagactttc gactggtttt tttactgttt ttctacaaat tatgatagga
    29401 tacatagtag gaagaaaggt gacataattt gaagtatcag caagtggaaa tagctttttt
    29461 tattgaccgc ccgaatcggt ttatagcaca ttgtatgaaa aaagacggag aaataattat
    29521 tgcgcatgtc aaaaatacag gtagaggaaa agaggtcctg ataccaggcg ctgaagtggc
    29581 tattgtattt gctcctggac ctaaacggaa aaccgcctat gatctgattg ctgtaaaaaa
    29641 gaagggtgac tggtttaata tcgatagtca attgcctaat cggctggcaa ttgatggtat
    29701 tttagacggc acgatccttt taccaggatt aactggtcca tttacggtat acaagcgtga
    29761 agtaacttat cttcactcca aatttgatat ttatttagag acggtggtcg gacaaaaagc
    29821 atttgttgaa gtcaaaggta tgacacttga aaataaagcg attggtgctt ttcctgatgc
    29881 gcccacactc agaggattga aacatatagg agaattaacg tatgccgcac aagatggtta
    29941 tgccgcttat gtcttgttta ttgctcagtt cgaacatctt catctggcaa cgatccatga
    30001 agaaatgcaa ccagcactag ctgatatggt tcgacatgcg cagcaaagcg gagttcaaat
    30061 cctcgcctat aattgtcaag tatcggcaga tgaggtaact gtcaagcagg ccattccatt
    30121 tgatgtagat atcccttttg aagatcctat acaatagaaa gaaggtaaaa taatgatcaa
    30181 cttaggtatt atcggaacaa attggattac tcatcaattt gtccaagcag ctttaagcac
    30241 aaaaaaatat gaattgacag cggtatattc tagacatttg gaaaaagcac aggaatttgg
    30301 ttctcattat gaaggggata tcgaattcgc aacagatttg aagacttttt tcaatattac
    30361 tcatatggac accatttata ttgcttctcc gaatagtctt cactttagtc aagctaagca
    30421 agcaattttg gcaggaaaaa atgtgatagt tgaaaagcca gctttttcga cgccaaaaga
    30481 aatggatgaa atcatccatt tggcaaatga acacaatgtg tatttttttg aagcagcaag
    30541 aaatatccat gaatcaggtt ttaaaaagat tgccgattac ttaccattaa aagacgagat
    30601 aatcggtgca aatttcagct atatgaaata ttcttcgcgc tatgaccaag tattagatgg
    30661 agaagaaccg aatatcttct cgactcattt ctcaggtggt gcattgatgg atttaggtgt
    30721 ttaccttgta tatgcagctg ttgcttggtt tggaatgcca aaagaagtcc attatttccc
    30781 aagaaagatt tctactggtg tagatggtct tggatggggg atcttacgtt atgagaattt
    30841 tgatgtatcg atccagccag gtaaaatcgg tgattcttat ttaccgtcag agatttattt
    30901 tgactcagga acgctagtga tgaatggagt caatgcaatt gaacaagcac aatttcatga
    30961 tcgcgcgcat aaagaaatca ttgatttggc ggtaacagct gcagaaaatc caatggcaga
    31021 tgaagcagag gattttgcca atgtcatggc tcatccgaaa gatccagctt ggggattgtt
    31081 gtatgaagag tggatcgagc tagctagaaa tgtgaaccaa gtgatttatg atttaagaaa
    31141 aaatggcggc atcaaattcg atgcagataa tgaggaagat ttttaatgaa cgaatttgta
    31201 caagtatttc ccgaaaattg gcagaaaaaa tatgaggata aagggtttat ctcaccttct
    31261 cttattcaac gagcagtttt tcagccacta agtaataaac aaagtatagt agcggtttct
    31321 cctacaggtt caggtaaaac actggcttat ctttggcctc tactactaaa cgtggaacca
    31381 ggtgaagcaa gtgcgttggt catttttgct tcttctcaag aactagctat tcaagtagca
    31441 gatgttgcac gtgaatgggg aaaagacaaa gagttgaaag tccaatcttt ggtgggcgga
    31501 gccaacgtca aacgtcagat tgagggtttg aaggaaaaac cagaaatctt gattggtaca
    31561 ccaggaagaa tattggaatt gatgaaagca aaaaaaataa aagcacatca agtgaaaaca
    31621 atggtatttg atgaagcaga ccagctattt gacgctggga acagccaaat cattgatcaa
    31681 attcttcatc aagctccaac agagtatcag ctagcttttt tttcagcgac agcagatcgt
    31741 tcccttgaaa gcattgaaaa aatcactggg aaaacgtttg agacaattga cgtgacagca
    31801 gaggacgatt ctcgaaaagg actgagacat tactttttac gtgtaccgat acgaaagaaa
    31861 gatgactact tgagacgtct cacgcatata gaagattttc atggtctgat tttctttaat
    31921 caactgactg aattaggcgt aatggaagaa aaattgctgt atagaggtgt accggtagga
    31981 agtctggcct ctgatcagaa caagctactc cgaaaagcag cgctagaaca attcaaaaag
    32041 aaaatggtca gagcactttt gaccacagat gtggcggcta gaggtttaga tattacaggt
    32101 cttccttatg tagtgaatgc agaagttccg ttaagtgaag aagcttattt gcatcgttcg
    32161 ggaagaactg gtcgaatggg taatgaagga atagtaatta cactcgtaca agataataat
    32221 ctaagagatt taaaacggct tttacgtcca tcaaatataa cacttgaaga gattttttta
    32281 tacggaggta agctgcaaac tgagcagcca accaaagaag atgcagctcc tgcagtaaat
    32341 aaaaagtatg cgaaaacttt ttctggaaag gagcagaacc cagaatataa caaagaaaaa
    32401 ataagaaaaa aagttaaaaa taaatcaaaa aaagagagaa ataaaggagc aaggcgcaaa
    32461 taagcgcctt tttatgctat gattatgaaa acgggaacaa aattggttgc cagaggaggg
    32521 gttatgtgaa agaacgtcaa gaagagatct tgcagctatt gacagaaaac caacaaggat
    32581 tgacagcaag tgatgtagct gagcgcttaa ctatcgatcg aagcaatgca agccgatatt
    32641 taagtgaact atacaaggcc catcatattg taaaaaccgc tggccgacca gtcgtttact
    32701 ctttgccaac ggaaaagtca aaatctgatg aagtgcatgt cgactcttct acgcaagtga
    32761 cttttgaaac actcgttgga gaaaatgatt ctttaaaagt cagcatacag caggcaaaag
    32821 ctgcgattct ttatccaccg cgggggttgc atacgattat ttttggagaa actggtacag
    32881 ggaagtcgat gtttgctgaa tgtatgtatc attttgcaat cgattcagag atgctttctg
    32941 cagatgcgcc atttgtttct ttcaattgtg cggattatgc tcaaaatccg caactgctct
    33001 ttggacacat ctttgggatc aaaaaagggg cttacacagg agcagctcaa gatagtcccg
    33061 gtctaatagc caaagcagac ggcggtatat tgtttttaga tgagatccac cgtttaccgc
    33121 cggaagggca ggaaatgctg tttactttca ttgataaagg tatttaccgt ccgcttggag
    33181 aaagtgcaca agtacatgaa gcatctgttc agatcattgg ggcgacgact gaatcttcag
    33241 aaagtttctt atctacgttc aatcgacgga ttcctatggc aatcaccttg ccgaatcttg
    33301 cttcacgttc actggatgag cgttatgaaa tcatctctct ttttatcaaa caagaagcga
    33361 atcgtttgaa tcaacgtatc gatatcgaaa aagaagcgat tcttgccttc atgctttatg
    33421 atgcggaagg aaatatcggt caagtgaaac gagacttgaa actagtctgt gcaaaatctt
    33481 ttttacatta ccgtactcat catgagaaaa agctaatcat tcgaaaagaa gaactttccc
    33541 ttcaagtaca aaaaggatta ctgaaaatca aggaaatacc ggaacgtctc gaccgattca
    33601 tggacagcaa aagtcagtat ttgacatttg aaccaggatt tgctgatgtt gtctggtcac
    33661 aagatccaga acgaaacatg caggtctata acgatattga ggaaaaagtc acttctcttt
    33721 ccgaaaccgg tgtggagaac attgatctag agaccctcat ttcaaaagat gtggatgctt
    33781 atttccagac atatgtcaaa gaattgacta aaagttcgat tcccaaagat cttttgccag
    33841 atgaaatctg gcggttaacc aatcaattgt atgatgtggc tgaaaaacgg ttagaccgta
    33901 tttataatga aaaagctcgt tttgcttttg ctttgcatct gcaaagtaca ttggatcgtg
    33961 tgagagaagg tcatatgatc gtccacccag atttgaataa cgtacggaaa aattttaaga
    34021 gggaattcca ggtagcaatc gatctttcaa cgatcattga agaagaagaa caagttgaga
    34081 ttccttttga tgaaatcggc tttattagta tgtttctttc tatcaatcta ggagaaagtg
    34141 agcctgttaa agaaaacaaa gtagatgttc tcatattgat gcacggcaga cagactgctt
    34201 caagcatgtt ggagatggca caagaactat taggaacaaa agtagggaaa gcttttaata
    34261 tgccactggt tatggaagtt caaacaatgt atgaagaagt gttacaatat gtcaaaaact
    34321 atcaagagca tttgacgaat ggcctactct tgttaacaga tatgggatca ttaaacacct
    34381 tcagtgattt gatttatgaa gagactggca ttcggacgaa agctatcacg atgacaagta
    34441 ccatgattgt gatagaagcg atacggatgg caagtgttgg aagaagctta gaagatattt
    34501 atcaaaatat ccagctttcc tttgaatcga tcgtacgtga tcagttcaaa gcggaaaagt
    34561 ctgaaacttt acagcgtaaa aaagccgtaa tcgtgacatg ctttactggt gaaggagtag
    34621 ctgccaaatt atatcagcgt attatgccag tgattaatca aaataaggta gaactgatcc
    34681 agatgcagtt tttagaacgt gaatcattca aaaaacacat cgatgcctta ttggaagaat
    34741 atgagatcaa agcaattgcg ggaacagttg atgttgatta tcaaaatatc ccattttttt
    34801 cagcctatga cgtttttgat gatgaaaaac taaatatttt aaagcgtata gctgatgatg
    34861 agatcccgac agctaaaatt gtcgattcac ttgctggatc attaagcgat gtcttttcta
    34921 ttgatgaact gatccgttca ttgcaaaaga cagttcagca gatccagacc gatttgtcga
    34981 ttatcgtgga acctggagta gatactggaa tcgttatcca tttagccttc atgttagacg
    35041 gattgatcaa acaagaaagc catcggcaat ttgagaaact gtctattttc tcaaaaattt
    35101 atcgtttgga aatggatatc gttcgggcaa acttgatggc actagaacga aaatatcagg
    35161 taaaaattcc agaagatgat atcgcatttc ttacacaaat gtttcttgaa aataaaataa
    35221 aaaaatgaac acactaatca tactgtttac acactaaaca tctgtgtgcg cactataaaa
    35281 aagagctaaa aagcatgaaa acgctatttc agctcttttt tttattggca tgcttcttgc
    35341 gttatataaa agtgtaaaaa aataaaagga gtgttaaaaa tggcaaaaaa aacaatcatg
    35401 ttagtttgtt cagcagggat gagtacgagt ttattggtca caaaaatgca aaaagcagca
    35461 gaagaaaaag ggatggaagc cgatattttt gcggtctctg cctcagatgc agataataat
    35521 ttagaatcta agaatgtcga tgtattatta ttaggtcctc aagtacgttt catgaaagct
    35581 caatttgaac aaaaacttgc gcctaaaggt attccattag atgtcatcaa tatgcaagac
    35641 tatggcatga tgaacggaga aaaagttctt gcacaagcag aaaatctaat gaaataaatc
    35701 aagtatgcag ttgggggtat aacaacgtgg aagatcaaca aaatctagaa gccatcatgg
    35761 ggttaatcat gtttggcggt aatgcaaaga gcgatgcaat ggaagcgatt gcggctgcta
    35821 aaatcggtga ttttgagtta gcggatagga aaatcgctga tgcagaagaa tcacttatac
    35881 aagcgcatca tgcacaaaca gacatgttga cacaagaagc acaaggaaat catatgcaag
    35941 tgacattatt gactgttcac agtcaagatc acttaatgac atctattgcg tttactgatt
    36001 tagcaaaaga aatcatcgaa ctttatcgtc gcgtatacag cgaataaata caacttcttt
    36061 tttgaacgga ttttttattt tgttataagg agaactctct ttatataaaa aacttatagg
    36121 aggaagtaaa aaatgaacgg actatcaagt tggatggaga aatacatcct gccggttgcc
    36181 gcaaaaatcg gagcgcaaaa acatttagtt gctttgcgtg atgcttttat cggcacactg
    36241 cccgcaacaa tggcgggatc agtcgctgta atgatcaatg caattattcg tgatttaccg
    36301 caacagttta acagtaccta tgctgacagt ttagcagcag acaaaggaat ctttgccgta
    36361 ttgaatgtca tcatcggaat caatggtttt gtctggaacg gtacactagc aatcgccgga
    36421 ttgatctttg ccttttcttg ggggtataat cttgcccgag cttatggagt aaatgatcta
    36481 gctgggggga tcgttggttt agctaccttg attcaaggta tttcctttgg tccagcatta
    36541 tcagcagcat tagatgtgaa tgttccttca aatgtcgcag atgcaatcaa tagttctgag
    36601 attggtgcaa cgattgcaga tggcacactg tcagttggat tatggggttg gctaaaacta
    36661 gatcatttga atggaaacgc ttattttaca gttatgatca tgggagctat ttcagtcatt
    36721 attttttgta aattgatgct tgccaacatt acgatcaaaa tgcctgattc agtaccacca
    36781 gcagtatcta aagcatttgc agccatttta ccagcgacga tcgctttata cgtcattgct
    36841 attatcaatt atatcgtagg tcgtgtagct gatgggcaac tgatcattga tttggtgcaa
    36901 aaatatattg cagaaccatt cctaggatta tctcaagggt tgggagcagt attgatcgtc
    36961 acagtatttg ttcaagtatt ctggttcttt ggtattcatg gaccaaatgt attggcacca
    37021 gtgctagagg gaatctgggg acaagcacaa ctaatcaata ttgatatttt ccaaaaaggt
    37081 tataacggaa aaactggtac accagccgtg ttagatgcaa tcgaagatgg aaaagcttat
    37141 atgtgggtcc gcggatcgtt cgatgctttt gcatggtttg gcggatcagg cggaacgatc
    37201 gtcttactga tagctatctt gattttctct aaacgtgcag attatctaac agtagctaaa
    37261 ttatccattg ggcctggtat cttcaatatc aacgaaccag tgatgtttgg tctgccaatc
    37321 gtattgaatg caatcatgtt tatcccattc ttgattgctc cagtagtagc aacagcaatt
    37381 ggatggacag ctacttatct tggcctagtc gctccggtat cgcagcaagt cacatgggta
    37441 gtaccgccgt tcttattgtc attcttagca acaggagcgg attggcgggc accgatcgta
    37501 acattagtat gtatggtcgt gactttcttg atttgggcac catttgtcat ctctgcaaac
    37561 aaaatggacc ctgccgcagg cgaacaacaa taagatgaac gtgaggagcg ggacgcttta
    37621 agtttcgttc ctcataatga attttcgagg aggaactgaa atgattacaa ttgccagtgc
    37681 aatgcaacaa gatgaaatca aagaattgtt agaaggatac aatgaagaag gaatgacttt
    37741 tacatttaaa gaaaaacaag gaatcaagct gttctttgaa acaagtgcag aagatccgga
    37801 agttgcggct aaaaaagcaa aggaactaat caaagcacag ccttggggaa cagtccttta
    37861 ttttcaatca acagctgttt agaaaatggt ttaaaaagat aggatggttg agatgataaa
    37921 aaacggttta tttttatgta gtattggttc tttgatcgtg ttgtatagtt tgaatccaca
    37981 ttcgcaaagg tatgatttgt atagtatggt cacaggtctt tttctaacag gattaggaat
    38041 ctatttcttc atgaagggga aaaaaagaga agaagcaaag gagaaaaata actaatggaa
    38101 atcacgaaaa aattaaatat tccagcatcg tttttttacg ataaagttat ggactctgtc
    38161 cttttcgatg tacgtaaagc gaccggcaaa tcagtaacta gaaaacagct gaaaaatttg
    38221 gagtacgtca aacaattctc gcaaaataac cgggctcgca taaaaatcga agaagtcatt
    38281 gaaaaccagt cttataagtt tcgtacatcg acaacgaaga atgaattcgt cgttcattat
    38341 caaatcaaac cattggacga ttcgacctgt gaggttcatt acacagaaaa aatggagtct
    38401 tatggcatgc ttcaaaagtt gaatgatatg cttttaggaa caatcttgat gtattttaaa
    38461 cggagacgtt tcaaaaaaat gctagatatg atcgaagcaa cgtattaaat caatgaaaca
    38521 tggtactatt tagttattga aaaatgaagt aagctagaag gaatttttca taggaagata
    38581 gatctttcct gcgaatcctc actagctttt ttgtaggaag gggcagcaaa atgacaaaca
    38641 aagtaagaga gctaggtgta tcttcgattg aggaaatgtt tgatttagcc acttatgcat
    38701 ttaatggggc agatacacct gaaaggcgag aacgttttcg tacacttgcg gagaattctt
    38761 ggaattacgg attttttgat gaggaggatc ggctaacaag tcaagtcatg gcgacccctt
    38821 ttccagtgaa tttttacgga caagagtacc taatggccgg cattggctat gttgcttctt
    38881 atccagaagc aaggggacaa ggcgggatca atcagatcat ggaaaagatc cttgaagatt
    38941 gccgtgatcg taaagtatgc ttatcttatc tggcgccatt ctcctatccc ttctatcggc
    39001 gctatggcta tgaacaatca tttgataaaa tcagttatca actaaacagt cgtgatgtgc
    39061 cgtttatcaa aaaaaaatct ggaaaaataa agcgtatgga ttttgaagat gcaaaagctg
    39121 caatcagaag aatttatgaa cagatgccgg aaaaccaacg agctggtctg aagcgcgaag
    39181 actggtggta tacgtataaa ttcaagcaaa aaggcaatta ccagtttgct ttgcattttg
    39241 atgataatga ggaagcaacg ggatatatcg tttatcagct tgcaacgtct tcctttatta
    39301 ttgcggagtg gggatttttg gatcatgatg ctttttgcag ccttgttcgc ttcgtaagtt
    39361 ctcacaatgg tgcttttgaa acatttgaat attcatgcgg ctacggcggt aatgatcaaa
    39421 attatctatt agaaaatcca tttgcttctg taatgatcac accttatatg atggcgagga
    39481 tcgtagatat ttctttgttt ttggaaaatt atccatttaa aaagagacaa gctgcatttg
    39541 ccctacaaat agaagaagat cattatgcga aatggaatga aggaatcgtg gaagtaacaa
    39601 tcgaaaatgg aaaaaatcat ctctcaaaag tagaacaaac aaaactccca atcgttcgag
    39661 gaagtatcca acatatcacg cagctattat tagggtatcg taaagtggaa gagttggtat
    39721 tccaagaaag aatacaggtg acaaaagaat atgaagaaac gttaagctca atcttgccac
    39781 agcaacagcc aattcttaat gactattttt gataaaaaac gggtgatgac aatatttttg
    39841 tcaccaccct tttgtttttc atttggcatt tgaaggaaaa tgttaaagta aatcctcttt
    39901 tttcgtaaat ttgaattttt tgttttcttt tgttttaggg ttttcttttt tgtggtcttg
    39961 gattgcttct ttcgtaatct tttcccgatt ttttttagat gaatctaagc gttctgcgta
    40021 tactttttct tttggcttat cattcattgg aaaggacctc cctacattat tcattctacc
    40081 tctagtttat catctgttaa ggactagtgg aaacgttttg cctaacttat aagctgattt
    40141 ttttagcaga taagcttgca agtaaaaaac aaacaagcta caattgattt attgaaattg
    40201 ttcctgtagc tcagtaggat agagcaatcg cctcctaagc gataggtcgc tggttcaatt
    40261 ccagtcagga acgtataaat aacccctgta tcctttgcgg tacaggggtt tgttttgtat
    40321 ttgtgccaaa aatgtgccaa actagttatc atttttatat aaactatcga acacagtatt
    40381 gattttttct ttatcctgat cttcaagctc tcgaacaata tgaagataag tagacattgt
    40441 agtttctaaa ctgctatgtc ctaaacgttt cgatacgcta agtatattta ctccttgata
    40501 aagtaaaata gatgcatgtg tatgccttag tccatgcaaa gtaaattgct tttcaatccc
    40561 taattctttt aattttttgc gcaaatattt gctgacggcg tttgatgaaa ctaatccatt
    40621 ttttaagttg aaaaaaacaa aattatgagg gtttttgatt tcaaaattct cgaataattc
    40681 tttttgattg attttaaact tttttaagag atcaatcgta tgtttatcaa tggaaatttt
    40741 cctatttgaa gtttcgtttt ttgtttttcc ccattcattt aatttataat tccatgtttt
    40801 attaatagtg atgatttgat cttcgaaatc gatatcttcc catgttaatc ctagaagttc
    40861 agcaaatcgc attccagtgg caccagcaac taacacgagc ataggagaag agtactgcgc
    40921 agaaaggttt tcttctacga gtttcattaa agatttaaat tgatcataat ctaaatattt
    40981 atcttctttt ggcttcaatg aatcttttcc tttgattact gcttttctag ttggatcaaa
    41041 cggaattaat ccttcttcta cggcatcttt caacgacgct ctaatatggt tattaaactt
    41101 aataacggta gattttacat ggtctttagc atacttattt aaaaagcgtt gatatcccac
    41161 tctatctaaa tcagaaatca ataccgctgg catatatttc tttatgttca ttaacgtatc
    41221 ttcgtatttt ctataagtga taggagatac tgttccttct ttataaagtt gcatccaatc
    41281 ctcaaagtaa tctgataata atagattttt gcgatcccct ttaaggcctt tgttcaagtt
    41341 atattctaat tcgttggcgg catcttttgc ttctcctttt gtcttaaatc ctgattttct
    41401 tatcttcgtg tattttccgt catctttttt gtatgaaatt tcgtattgcc aactatttcc
    41461 acgtttgact aatcttgcca taattgaatc agctctcttt ctctgataca ataggaacta
    41521 taaagaagcc cattgtttgg gttttattat tttttgcaca tcctttttgc ttgccggcgc
    41581 aaggatgtgc tatttgtcgt tttaatggta taattggatg tgtattagat catccaatca
    41641 gaggtgttta ttatgattaa agtgattttt aaaagaataa ctgaacttcc agagtttaga
    41701 ttatttgttg attattggta tgtctggttt tttattgttg cattgctgat aggtctagat
    41761 gtcatattag aaagaaaact taagtaagag ttgagttaat aaattgaatt cctaaatgag
    41821 atattttgta gcccatattt tgtttgccga catcacctaa tactatggag ctaatcgttt
    41881 tttctaacag accaaaatca gataatctat ttaagctacc atctattagc tcttgtgaat
    41941 agccagacat gctgatattt ccagtgaaat tttcgtttcc acgactcatt aagcatgcta
    42001 acaattgaat ctcaatcgat gttaattgat tcagtacaag aagataatac tcctcgttat
    42061 cccatgaagc gttgttaatt cgaagtaaac aattcttata aagattttta tacatatcta
    42121 ttttattttg tgcctttgat ttttctacct cgtcatttat cccttcgaat attccaatga
    42181 gttggtcctt gttctctaag ttgaaaacat cttctggaat ttgatcgcga atgttttcta
    42241 atgattcgtt taaatttttg tagaatttct cgatgcgttt gaaacgcttc tcgtttttag
    42301 acccaaaata aagtgactgc acaacgccac caatataagg aattgctcca ataccgcctt
    42361 caactattaa ttcaagatat tcttttttgg ttagtttttc attgctatta tccatagtta
    42421 cctcctattt tgtcatttcc tttacaagca ttgtgactcc tgatggcact cttttgtata
    42481 ggttttatta ttgtgcacaa tctaactctt agtggggcgt ggttcattta ttttttactt
    42541 actttactat taatcatatc ccattgagca taggattttt ctatgttcga tcctcttgaa
    42601 ctaatttctc ctgtaaaagt tactttatcg ccctttgtaa aggtatcagc attgtttaag
    42661 tccttcgcaa aaattacata tgaaacttta ggtgttgcag aaatatcatt ccaactttta
    42721 tcatcgtact ctttgtctgc tataatggct attctagtag tcattgattc gataacagtt
    42781 ccttcaaaag tatatgtttt accactaata tatttagtaa aagctgtatt ttgctcagta
    42841 gctggagtaa tagaataata actatctaag aaagcacccc agtcatgatt atttgtctct
    42901 aaaaattctt caaattgagt taggctttta ttgctgctgt tagatgtagt agtttgagta
    42961 tcagatgatg agctttcctg tttgttattg tcatttgcct cgtttgatga ggaaagcgag
    43021 aaacctacgg tagtaaaaat aatcccgata ataaggagaa tcataggtgt tttcttattt
    43081 gtttttttga taaatcttac aattaacatg attaaaccta taataaataa aacaatacca
    43141 gtaaaaccaa atataccgtc cattaataga tcctctttct cttttgatat tgaatattat
    43201 aaaccaagca attctttttt cttaaaatcg aattcttctt gattaataat cccttcatct
    43261 aacaactctt tatacttttt aatttcatct gcacttgaaa tttgtgtttc tttggaatta
    43321 ttatctaata tagattgttg cttctctata tattttttta aattttccat ttcattccaa
    43381 tatttttttg taaacataac ggtgttttca tctttcgtgg ccgctaatat acctccgcga
    43441 ctttcgtttc cgcctagcaa ggtaaattgt atataaccat tactcatacc tggttttttt
    43501 aattgtacgg ctgatatatt tttaaagggg atagtttttt ctcctttaat accttgatta
    43561 actaaattta taaatccttt tctaataatt gaaatagaat catcagttac tttaatcaag
    43621 gtttttcctg gagattttat taaaatctct ttttccattt tttcttcctc atttctgtga
    43681 tatgattttt tttgtagaag ctcatatatg aggaaagagc ccgtgttgca gcacgggctt
    43741 ttttaatgtt ttgctgaaat cggttttttt aataattctt gatgtattca tacatattgc
    43801 cttgcgtaag aatatttttt cttaaaatag cattggcaga cagcataaca agtgtatctg
    43861 agcttattct gaatagaata atactccata aatttttcga gattaaactg agatttatca
    43921 gtcagttcgt tctcaatata gatatttaat aaaattagaa tagctatttt atctgcttct
    43981 gtttcaaatt ttgagtgaaa agttgtagaa gtatcgtaca aaactgaaaa ttcaaaatga
    44041 gaggcgctga aatgtgcaag ttcatgagat aaatgaaagg cttctgcagt ttcaccgtat
    44101 agattttcat tcaaaaaaat gattctgggt tttggatagt agaaaccagg ctctttcatc
    44161 tccatataaa ctactttcaa attgtattcg ctcaacattt ctttcaattt caaatacata
    44221 caaaccatca ctccaactat tcattttcct ctaaagcttt agcaattgca atcgctttac
    44281 gcattgtctc cttagatatt tcttttccat caaaagaaaa aacagtatcg tcttctgata
    44341 aatccacatg tttaggggtt tctctttctt ctctacctag aaggtagtct acagagacat
    44401 cgaaatagtc agcaatccga cttaattcat ctgaattagg agtattattt ttccactttg
    44461 ctagataacc gttagaatat ccaaaattaa tttctaactg ccgtatagat atctttttcc
    44521 tcttcgccaa gtcttttatt atttcatagg tattcatcga taaaacaacc tttctaaatg
    44581 tttacaaaaa aagtttagaa aaatacgcag aaataatttg actaattctg agtaatgagc
    44641 tatactatat cttgtaaaca gatttaacaa ctaaaaagac aacaaaaaac actattgata
    44701 tgtaaatgca gaccgccaag aaagctttaa aatcaatgtt tttatgtctt atttaattat
    44761 gctcttagta tagagtatta ctcagatact gtcaattgaa tttagaaaaa agttgttaaa
    44821 tttgtttacg aatataaaag aaaggaatga gaaatatgaa cacaccgcaa atttttaatt
    44881 tcgaacaaaa cgaagttcgg acaattttag taaatgatga accatatttt gtaggcaaag
    44941 acgttgcgag tgttttgggt tattcaaaca ctaaagatgc tttgtcgcgg catgtagatt
    45001 tggaagataa gatggggtcg cgaatcacga cctcaggtca atcaagagag atgacaatca
    45061 tcaacgaatc tggtttgtac agtttaatct taaaatcaaa acttccctct gccaaaaaat
    45121 ttaaacgttg ggtaacaagt gaagtgttgc cagcaattag aaaacatgga ggttatctaa
    45181 ctccagaaaa agtagaagaa gctttgctta atccagatac aatcattcaa ttagcaactc
    45241 aactaaaaga agaaagaact ggaagattaa tcgcagaaca aaagattgca gagtacgaac
    45301 ctaaaatctc ctatttagat agcatattat cttctacaga ttcagtaaca attagtcaga
    45361 ttgcagcaga ttatgggatg tctccacaac agatgaataa attacttcat aaactaggta
    45421 ttcagaaaaa agtcggtaac caatggttat tgtgcaaaaa acacatgaac caaggataca
    45481 caaaatctca tacaactgag atcccgaaag ccgatggtgg cactaaaatt gtaatgaata
    45541 ccaaatggac acagaaaggg cgtctattta tctacgaatt actaaaaaaa gaaggatatt
    45601 accctcaaat ggatttagag gaaattggtt agaaaggagt tttagtatga ctgacattgc
    45661 agaaatcact caacgagata gagaaaaaat caaagaatat gtcgaaagtt caaagttctt
    45721 aacttacacc atgcttgctg aaagatttgg aattagcaaa agctacttat ctttaatttt
    45781 aaacggtaaa aagacttctg cagaagcaaa cagaattata gattcgatta tcactatgta
    45841 cgaattgtaa agggaggaac agctaatgca atatctagaa gcaaaaatcc caattccaga
    45901 aggctatgta attatctccc aagtggatta tgaggagtta aaaaaagctg atgatactgg
    45961 tagatggatg acgttgccag aagtgctaga acggattaac agaaaatatg attggtttac
    46021 ttctagagtt ttaaagaacc caagatatag aaatattatc gatatcgaga aaaacaaaaa
    46081 tggatttgtc tattatccag ttgaaggaag agacacatat ctatttttaa gaagtaaaac
    46141 acttgaattt ttagaaacaa atttttcgga aatcttaagg aggcaagcgg atgggaaaat
    46201 ttaatagagc gctagtgttc agcgcaccgc taatcatcta cgctttagga ctttggggaa
    46261 gcaggcaagc gttgatagga acgattgttt acatggtttg gatttttatg gggcttgatg
    46321 aagctgaagc tgagtacaga gcgaaaaagc caaccgaggg ggctgactaa aaatgaatag
    46381 aaaagagaaa ctagaatgta tattactatt actcagttta attctttcac taatttctct
    46441 attgggaagt ttgtattttt gatatcaaca attttgtgga tttgggttct atttagagta
    46501 aatatcattt ttgtttggtg attaaggagt ttattaaaat taattttccc tacttcaaat
    46561 gcaaggtaac aagatatagc acttttagga ggaatattta gcggtacagc ttcagagtat
    46621 attctattat catcgactga tcctccttta gcaattaatt tcttaaatgg agtagctgac
    46681 catctgcggt ttaagttagc ggatgaaagt tctaaattaa ctaacatagc tggttctgtt
    46741 gaatagttag aaatgattac ttttgtataa atcatatctg cagcgaagta agaagcatta
    46801 agttcaactt ctagttgagg gcgcattcct tttattttta tccctgttaa aaacgtactc
    46861 aatataaaac ttattattga catccattga aaaattgtta agtgaaaaaa attcaaaata
    46921 aacaccacca gtttttaact aaattatacc aaaaaggaga gaagaaataa tgcaagaatt
    46981 agtaattttg aaaaataaag aagctgtgac tacgagctta caagtggcag aaagcttcga
    47041 gaaaaaacat caacatgttt taagagatat tgatgcacta aaaaaagatg tgtccaattt
    47101 tggacagatg tttgtagaag gtaatgaacc agattcatat ggcagaaatc gacgagtttt
    47161 cttcattagt agagatggat ttttcttgct ggctatgggg tttacaggaa agaaagctat
    47221 ctacttcaaa caaaaataca ttgaagcatt caacgaaatg gaagatgtta ttcgcaagaa
    47281 tactgttcct caaacaattg aagatatgat gatctatcaa ttagaagaaa tgaaagatgt
    47341 taaaaaagat gtttccatgc ttaaagatac tatgcgaatt agcggacaac aagagtttga
    47401 aattaagcaa aaaggaaata tgaaagttat gggagttcta gggggaaaag aaagccgagc
    47461 ttatgaagaa atcagcaaaa aagtattctc aaaattttgg tctgaattta aacgtacctt
    47521 ttcaatccca agatatggcg agttacctcg taagagattc gatgatgctg tttcatttat
    47581 tgaaatgtgg ttgccagaaa ctgcgatccg tatggaaatt gatcaactga acagacaaca
    47641 gagacttttt ggtgatgaca atgaatagag ctgaagcgct aagaataggg acggcaattg
    47701 ctaattgctg gtggaaatac tataaaccaa tcatcctaag ccaacaacat attgacaagc
    47761 aaaaagcatg gcaacaaata aaaaagtgac tccgccggca agcaaagagt cacaaagaaa
    47821 acacatcata aggagatttt agcatatgga aaaagaactt tccactctag atcaatattt
    47881 gattgatcct gattggggca agccgaaaat tgaggaaaca agtggtcgaa aaatcagacg
    47941 aaatcttttg acgaatgaag aactagcttg ggatcaagat gatttaggca accatgtaac
    48001 tatttgggat catgtttatc ttatccatct atcgaagcat tcgaataaac ctgaatatat
    48061 ttacgtcatc gaagatggct tgattgatgc gctagaagag tacgacagag ataacttgat
    48121 tgatatctct tattacggac caggtaagaa atacattgct gaaatggagg cagaatttga
    48181 tgagtgaaat caaagggaca acgaactttg aaaaactttt tagtcgtaag ttaaataaaa
    48241 ttctcaagaa aaaaggaaat tttgattatt tatcttgggc tcacgcgtgg gagattatga
    48301 aaaagaatga tccacaggca acggtaacta ttaatgagta caaacactac agggttgttt
    48361 ctggaactca tcaagacttt cttgttgagg aatataaacc ttttcttatg gatgaaactg
    48421 ggacttatgt atctgtctca gtaacggtta aaggacacac ggaaaccgaa ttatttcctg
    48481 ttttagatta tcgaaaccaa ccagttgtta aaccaaatgc aatgcaaatc aataactcat
    48541 tgaagcgatg ctttgtgaaa gcattggctc tacacggact gggattatat gtatttcaag
    48601 gggaagatat tccaacacca cctagaatcg atacaaagaa attaaacatg ctagagacga
    48661 ttctagaagc tttcaatgag cagatgggta aagatatgac caaaacctta atcgaatatg
    48721 ttaatgagca gacagataaa ttagggctct tagctgataa cgttgaaact attgaacagt
    48781 taagctatga gcaatgtgcc ttgatggagc gagcaatagc agctaagaga aaagaattag
    48841 ataagaagtg atatgagtgt ttaaaccatt aatcgattca tattcagcgg ttctgaaaaa
    48901 gttcaaagga aaagacataa gcgcaaccat caatgaggaa gtgaacattg atcgactaaa
    48961 gacgatgtat gacggctacg atggtgatcg agtcattgaa attcgtttta ttgatcctag
    49021 acgtttcacc gtacagcaac gaaacttcat ctatgcgctg ataggcgata tttttatcga
    49081 tacaggcatg ccaacggact tctggaagga attcttctac tttcgttttg aaggtgtcac
    49141 agggcgcaaa ataagcctga aagacgaatc gaatacaact gtgagtgatg ccaatgtctt
    49201 agcaaatatc atcttagatt tcatctttga acatcatatt cctttcaaag aaggctatga
    49261 gattttacct gcgaatcaag aatattactt ctacaaatgc atcacaaaaa gagtctgctg
    49321 catctgtggc aaaacaggag ctgacatcga tcactttgac aaagcgctag gaagacgaaa
    49381 gcgcaaagaa gttgatcatg cagagtacac atttgcagca ctctgcagaa tccatcacac
    49441 agagaagcac aaaataggtg tgattaattt caaaaataaa tatcaaatca aagggatcaa
    49501 attaaaccag aaaacaatca aaaagttaaa tattggaggg taaaaatgac agaacatcga
    49561 agttattatg cgattatacc agccaacgta aggtacgaca aaagacttaa accaaatact
    49621 aagttgttat acggagagat aacggccttg tgtaatgaaa gaggcttttg ttgggcaggc
    49681 aatgagtact ttgcagattt atatggtgtg aataaagaga ccatatcgcg atgggtaagt
    49741 gatttgatta agtttggata cttgaatcgg gaaatcattt acaaagaggg taccaatcaa
    49801 ataatcaata ggtacctacg aattaatcaa taccctattg acgaaaaacg caatacccct
    49861 attgacgaaa aagtcaaaga taataataca tctattaata atacatttaa taatacaaaa
    49921 gaatatataa gagagttacc gccttcgaaa aaatcgaagg ctaagcccgt ccgtcataaa
    49981 tacggagagt ataaaaatgt tcttttgtca gatgagcaga tggagaaact caaaacagaa
    50041 ttccctaatg attaccaaga gcgaatcgaa cgactgtcag agtattgtga atcatctggt
    50101 aagacttata aaaactattt ggcaactatt cgaagttggg caaggaaaga aaaaagtgaa
    50161 cctaagaacg caagcagtgg atacaagcgc acaggaagac gagagaagct tcctgaatgg
    50221 gcaatcgacc aagaagccta tctcaagaaa aaagcgctag aacgagctaa tagacaatca
    50281 aaagcaccat tctaagaggt ggaaaaatga agatcgatta tctagaacta attaatgaaa
    50341 tagcgaatta taaaaagggc gaggaattag acgtcctgag agacgtatat gatcaactcg
    50401 aagaagctgg aattgaagga attaagaatg atcgttcgag ttggagtaag ctcagatact
    50461 atttcgcact ttatatcgat acaacacaat taagaaattt agcatatacc aaattactat
    50521 ttgttgattg tgttaaaggc ttgcaaaaac atcttaatga acttgagcag gtgtaatcag
    50581 atggacctaa agacatttac agcacagatt gaactaatgc atcaagaagc tttaagacaa
    50641 agtgtatcgt acgaagacaa gtggctcaac acgttccatg gcggacgtga gagcgcactt
    50701 gatcaagtac tcaaattact gaaaggagaa tgtcgggatg gataagaaag cggcaatgca
    50761 gcgaattatc gaattgactt attcagaaga ttggcaaaat gacaaagaag ctgcttcaga
    50821 agtgatgagg cttggaagag cgatgtgggc agacaagagc aacaagccaa gaccacgaaa
    50881 aatcgcaatt tggcacggtg acaaacttct agtgataggg acagctgaac agttagcaag
    50941 tctcacaggc ttgcacgaga aaatcgtgag gaaaagagca aggtgtggct acacagacgc
    51001 taagaagaga acgtttagat acgtggagga atcatcatga caacagaaga agtgattcaa
    51061 atgcgtattc gaagccttca gcgtgagatt gacgatctgg aacgaacaaa ggcagtgatg
    51121 gtcaatgaaa cggcgagaaa ggcaatcgat ttgcacatag agaatttaag aagggaaatc
    51181 catcgattgg aggaatgagc gtggataaga aagcagcaat gaaaagaatt gctgaattaa
    51241 ccaagtcaga atcttggcaa gaagacaaag aaatagttgc agaagtccaa aagctcggta
    51301 aatcaatgtg gactgaaaag cccaaacgga gaacgccgag aaaaattgca atctggcatg
    51361 atgaccgaat tttagtaaca ggtactgctg aacagttatc tgaaattact ggattaagca
    51421 aaaacattat ctgggataga gctaggagct tatggattga ttcaaaagga cgacagttta
    51481 ggtatgtgga ggagagataa tggatctcat tacacaatac agtgacatca tcctcaagaa
    51541 aatcatgatg aagattcaga aagataaaaa atcaaaagaa cgagctgaat tagttaagtt
    51601 agaaatggct gaaacaggag caggagtgcg aagtagcagg cattggaaag cagcagcaaa
    51661 cattgaattt tattacaacg aaattcaaaa agggttcgat cagatgcgtg agctggatcg
    51721 gcaaacaaat tggagcaaga aacttcatca agatcgtttc aaatttgtag agaagtataa
    51781 agaaatatta gaagagtatt tgaggaggac agcaaatgat aaaaaaactc gttcaattca
    51841 gcatggattt atatgatatc gaatcaggag caacactatc tgtggaatcg gaccatctaa
    51901 tcataaattt tggtggaaag cgccagatta ttttgtgggt agttgatgat gtactgtttc
    51961 cagaaattgt tcatgatttc gaagaatcaa aagcggttga gtttgaaata gtgaaaaaag
    52021 taatggaatt gattgaaaaa tacgaggagg acagcgaatg ataccgaagt ttagagcgtg
    52081 ggataaacga aagaacgtaa tgagagatgt agccgtcttg cattttacta aaaacggcaa
    52141 aacaaacttt attgaatatt ggataaatcc taccgaattg aaatcatatc acgtgcgaaa
    52201 catcgacctc atgcaatcca caggaatgaa agataagaat ggtgtggata tatttgaagg
    52261 ggatatagta ttagtcagcg tgcgaaatgg cttcgattac ttagataata aagtctgtat
    52321 tgtcaaaaat tcaatagatt attccggatt agtttgtgcc actgttgatg aagacttaga
    52381 gtatcaaatt tttaacacag agctgtttga agaatacacg tacgaagtca ccggaaatat
    52441 atacgagaat agcgagttat tggaggaaca gcgatgaata aacaggaagc aatcaaaaag
    52501 ttagaaagta ttaaagcgat aggaaatgat gcaatagctg cttgctataa cgagagtata
    52561 aattcaggta ttacgttaat gaaaaaaata gacgaaccgc agaaaccagt tattccacaa
    52621 ctcgtggccg gttggcttga gaaatctacg gaccctttta caaaagctga aaaaatagcg
    52681 tatttaatca aatctaaaga tggtgattca tattatttct gtgattggtt tgtacgagat
    52741 ggcatagtga cgcaagagca aggagaagaa ttactcgctt gggcaacaag acaatcatat
    52801 gaaacactat tgagcctata caacggctac gaggttgaga aagagccgtt atgggcaata
    52861 aagaatgccg atggaaacta tcttactaaa tgtgctttat ggggaaaaga tggagtaaat
    52921 tatagttttg aatgcaatcc atctcatcga ttgcttttca ctgataaagc aacagcggat
    52981 gctgcagcat tgttggtgaa tggaacagtg gaagaggtgg taga
//
LOCUS       DAFBQH010000009        51804 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.009, whole genome shotgun sequence.
ACCESSION   DAFBQH010000009 DAFBQH010000000
VERSION     DAFBQH010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.009"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(115..1362)
                     /locus_tag="LK694_000633"
     CDS             complement(115..1362)
                     /locus_tag="LK694_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBK5051525.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(1438..1584)
                     /locus_tag="LK694_000634"
     CDS             complement(1438..1584)
                     /locus_tag="LK694_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HBK5051526.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(1688..1999)
                     /locus_tag="LK694_000635"
     CDS             complement(1688..1999)
                     /locus_tag="LK694_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBK5051527.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(2001..2198)
                     /locus_tag="LK694_000636"
     CDS             complement(2001..2198)
                     /locus_tag="LK694_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HBK5051528.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(2592..4319)
                     /locus_tag="LK694_000637"
     CDS             complement(2592..4319)
                     /locus_tag="LK694_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HBK5051529.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(4312..5505)
                     /locus_tag="LK694_000638"
     CDS             complement(4312..5505)
                     /locus_tag="LK694_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK5051530.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(5692..6336)
                     /locus_tag="LK694_000639"
     CDS             complement(5692..6336)
                     /locus_tag="LK694_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5051531.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(6430..6564)
                     /locus_tag="LK694_000640"
     CDS             complement(6430..6564)
                     /locus_tag="LK694_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBK5051532.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(6566..8698)
                     /gene="feoB"
                     /locus_tag="LK694_000641"
     CDS             complement(6566..8698)
                     /gene="feoB"
                     /locus_tag="LK694_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBK5051533.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(8695..9168)
                     /locus_tag="LK694_000642"
     CDS             complement(8695..9168)
                     /locus_tag="LK694_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBK5051534.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            9569..9793
                     /gene="nrdH"
                     /locus_tag="LK694_000643"
     CDS             9569..9793
                     /gene="nrdH"
                     /locus_tag="LK694_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBK5051535.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            9952..12111
                     /gene="nrdE"
                     /locus_tag="LK694_000644"
     CDS             9952..12111
                     /gene="nrdE"
                     /locus_tag="LK694_000644"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBK5051536.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            12161..13126
                     /gene="nrdF"
                     /locus_tag="LK694_000645"
     CDS             12161..13126
                     /gene="nrdF"
                     /locus_tag="LK694_000645"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBK5051537.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(13215..14354)
                     /locus_tag="LK694_000646"
     CDS             complement(13215..14354)
                     /locus_tag="LK694_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK5051538.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(14663..15376)
                     /locus_tag="LK694_000647"
     CDS             complement(14663..15376)
                     /locus_tag="LK694_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBK5051539.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(15630..16331)
                     /gene="nagB"
                     /locus_tag="LK694_000648"
     CDS             complement(15630..16331)
                     /gene="nagB"
                     /locus_tag="LK694_000648"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBK5051540.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            16565..18097
                     /locus_tag="LK694_000649"
     CDS             16565..18097
                     /locus_tag="LK694_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK5051541.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQINWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(18288..19490)
                     /locus_tag="LK694_000650"
     CDS             complement(18288..19490)
                     /locus_tag="LK694_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5051542.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(19572..20261)
                     /locus_tag="LK694_000651"
     CDS             complement(19572..20261)
                     /locus_tag="LK694_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBK5051543.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(20367..20735)
                     /gene="rplL"
                     /locus_tag="LK694_000652"
     CDS             complement(20367..20735)
                     /gene="rplL"
                     /locus_tag="LK694_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBK5051544.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(20788..21291)
                     /locus_tag="LK694_000653"
     CDS             complement(20788..21291)
                     /locus_tag="LK694_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBK5051545.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(21495..21622)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(21701..22390)
                     /gene="rplA"
                     /locus_tag="LK694_000654"
     CDS             complement(21701..22390)
                     /gene="rplA"
                     /locus_tag="LK694_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBK5051546.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(22494..22916)
                     /gene="rplK"
                     /locus_tag="LK694_000655"
     CDS             complement(22494..22916)
                     /gene="rplK"
                     /locus_tag="LK694_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBK5051547.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            23259..23927
                     /gene="sdaAB"
                     /locus_tag="LK694_000656"
     CDS             23259..23927
                     /gene="sdaAB"
                     /locus_tag="LK694_000656"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBK5051548.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            23940..24812
                     /gene="sdaAA"
                     /locus_tag="LK694_000657"
     CDS             23940..24812
                     /gene="sdaAA"
                     /locus_tag="LK694_000657"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBK5051549.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(24903..25922)
                     /locus_tag="LK694_000658"
     CDS             complement(24903..25922)
                     /locus_tag="LK694_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBK5051550.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(26095..27000)
                     /locus_tag="LK694_000659"
     CDS             complement(26095..27000)
                     /locus_tag="LK694_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBK5051551.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(27059..27604)
                     /gene="nusG"
                     /locus_tag="LK694_000660"
     CDS             complement(27059..27604)
                     /gene="nusG"
                     /locus_tag="LK694_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBK5051552.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(27705..27875)
                     /gene="secE"
                     /locus_tag="LK694_000661"
     CDS             complement(27705..27875)
                     /gene="secE"
                     /locus_tag="LK694_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBK5051553.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(27899..28051)
                     /gene="rpmG"
                     /locus_tag="LK694_000662"
     CDS             complement(27899..28051)
                     /gene="rpmG"
                     /locus_tag="LK694_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBK5051554.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            28213..28857
                     /locus_tag="LK694_000663"
     CDS             28213..28857
                     /locus_tag="LK694_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK5051555.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(28913..29416)
                     /locus_tag="LK694_000664"
     CDS             complement(28913..29416)
                     /locus_tag="LK694_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBK5051556.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(29429..29475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            30013..30978
                     /locus_tag="LK694_000665"
     CDS             30013..30978
                     /locus_tag="LK694_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBK5051557.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(31077..32249)
                     /locus_tag="LK694_000666"
     CDS             complement(31077..32249)
                     /locus_tag="LK694_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5051558.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(32236..33381)
                     /locus_tag="LK694_000667"
     CDS             complement(32236..33381)
                     /locus_tag="LK694_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5051559.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(33395..34319)
                     /locus_tag="LK694_000668"
                     /pseudo
     CDS             complement(33395..34319)
                     /locus_tag="LK694_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            34436..35515
                     /locus_tag="LK694_000669"
     CDS             34436..35515
                     /locus_tag="LK694_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBK5051560.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            35547..36140
                     /locus_tag="LK694_000670"
     CDS             35547..36140
                     /locus_tag="LK694_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5051561.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(36250..36477)
                     /locus_tag="LK694_000671"
     CDS             complement(36250..36477)
                     /locus_tag="LK694_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK5051562.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(36580..39168)
                     /locus_tag="LK694_000672"
     CDS             complement(36580..39168)
                     /locus_tag="LK694_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBK5051563.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(39188..39847)
                     /locus_tag="LK694_000673"
     CDS             complement(39188..39847)
                     /locus_tag="LK694_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK5051564.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(39896..40399)
                     /gene="trmL"
                     /locus_tag="LK694_000674"
     CDS             complement(39896..40399)
                     /gene="trmL"
                     /locus_tag="LK694_000674"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBK5051565.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(40620..41486)
                     /locus_tag="LK694_000675"
     CDS             complement(40620..41486)
                     /locus_tag="LK694_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK5051566.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            41595..42236
                     /locus_tag="LK694_000676"
     CDS             41595..42236
                     /locus_tag="LK694_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBK5051567.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(42298..43659)
                     /gene="mgtE"
                     /locus_tag="LK694_000677"
     CDS             complement(42298..43659)
                     /gene="mgtE"
                     /locus_tag="LK694_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBK5051568.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(43677..44576)
                     /locus_tag="LK694_000678"
     CDS             complement(43677..44576)
                     /locus_tag="LK694_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBK5051569.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(44578..45375)
                     /locus_tag="LK694_000679"
     CDS             complement(44578..45375)
                     /locus_tag="LK694_000679"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBK5051570.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(45353..46057)
                     /locus_tag="LK694_000680"
     CDS             complement(45353..46057)
                     /locus_tag="LK694_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBK5051571.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            46188..46763
                     /locus_tag="LK694_000681"
     CDS             46188..46763
                     /locus_tag="LK694_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HBK5051572.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            46881..47558
                     /locus_tag="LK694_000682"
     CDS             46881..47558
                     /locus_tag="LK694_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBK5051573.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(47637..49445)
                     /gene="pepF"
                     /locus_tag="LK694_000683"
     CDS             complement(47637..49445)
                     /gene="pepF"
                     /locus_tag="LK694_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBK5051574.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(49621..50619)
                     /locus_tag="LK694_000684"
     CDS             complement(49621..50619)
                     /locus_tag="LK694_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBK5051575.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(50845..51498)
                     /locus_tag="LK694_000685"
     CDS             complement(50845..51498)
                     /locus_tag="LK694_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBK5051576.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        15619 a        10777 c         8970 g        16438 t
ORIGIN      
        1 taaatacttt atcgtgaaaa agaatgaaat tatcaataat tcgttctaac tgatttaatt
       61 gtataatttg atcttcacat aatattattg gataactcat aaatcctcct tccctcaaaa
      121 caacaaaata acttgagtag taaattgata cggatcgttt tgatagttca caaacatatt
      181 gggattcttt ttattgattt cctctatact agataaacct aacccttgat gtcccttttt
      241 tgttgttatt ccttttttca ttagagtatc tatagagagt agtatctctc tacaactatt
      301 cgatatcgaa atttctatct gtttatcatc tcggtaaata gctagagaca gttctctatc
      361 ttgactctct atagatgctt caattgcatt atcgagaata attccaagca ccctaataca
      421 gtcaaatatt ggaacaggaa cttcaataat agtacttgga cattcgaatt tacaacaaat
      481 cttattgtta tttgcttggt atagcttcgc tatcaacaaa cttttcaaat attcattttt
      541 tatatttttt aaataacgat attcaaactt attggttaga taagaattag aatagatctc
      601 taattctttt atttcttttt ccaatatcga atcatggttt tgattagcga tttcttttaa
      661 actcagcaat aaatttttat agtcatgtct aaatctggca aatttttctt gatctttttc
      721 cagtgaatca gtatattttt ttagataaac caattctttt cttttcaatt gctgttcata
      781 ttgttctcgt tgcctaatag ttattctaat aaataaaaat agaacaacaa acatttgaat
      841 gattaaaaat attaagacac caaacacaaa atgatcaaat acttgatatt gatgagcaac
      901 ataagaaaca aataaaccaa ttgtaagtaa ataaatcatg aatatagcag taagtttaga
      961 actggactgt tcaacaatcc tatttatagc taattttcta tataaccaaa taaacatcag
     1021 caaaaacaaa gaatcaagca ttatctggaa tactacataa ccataccctt ttatcccttc
     1081 aaatgaaaac atatacgtca taaccatgga agtaataata gatctgaaat catcaataag
     1141 catacataat agtaatttat ttaaaagcac tatattagtt ttcttctttt cctttaatat
     1201 aaaataactt ccgattatag gtatagctgc ggaaacatca gtaaataatt caacaaaaaa
     1261 agtgagtggt atcaataaac atacaaaaag atattttctt ttgttaagat cagtattagt
     1321 catataaaat aagagaaaaa agaaaaagtt tcccacgaac attcttaata gataagcttt
     1381 tgtatcgata gtaatcaaga gatcccctcc tgattttagg atatcataaa aaaaatttta
     1441 gttacacttt ttaaataatg aaaaaacaca ttccactaaa tttgatgccg gttttccttg
     1501 aggttttgtt ccagcacctc ctttaatttt tttaagctct tgatctgaac attgtttagc
     1561 atttagtcta tttttttctt ccattacgat tccccctaca aactataaat acttttatgt
     1621 cactgattca atatacctat ttttctaatc aaaaaatttt gagacatatt taatattata
     1681 agtatcatta aaattgagat ttatctccat aatctgctcg atataaacca tttattccag
     1741 cagttcttcc aatttcactc aaatttctta ttaattcctc ttgatattca gtgaatctta
     1801 ttttattagt ataagcagta aaatatagat aattcaccaa tctatttatt aaaggaccgg
     1861 gatctaattc atatttttgt ttttctaatt ttaaatatac cttttgtaaa acctcaagaa
     1921 tatcagaata tttaggatct ttacttatag atatatcatt atataattca tgaacaattt
     1981 gcttagcatt ttttttcatt ttagcacttc cctggaattg ctccacctaa aaaaccacct
     2041 atagacattc ctgcaataca agtagttgcc ttggcccaat cgaccgtaca tttattttta
     2101 gtgcaataca ctccatttcc ataatatttt ccactatgag tggtaccccc atagataagt
     2161 tgtgtctctt taatagacaa aatttttaaa tgtttcataa tctaatcacc cctaaattaa
     2221 tgtttttaga ctattattct aacattcatt aaatttcaaa aaacaattcc ttcttgaaac
     2281 gtagcaaaat aattcctgaa taagaattta atcactttta agtatataaa ctatgtatat
     2341 tctttaatat tttttgagaa tgcacttcgt acataataaa ctttaatcac attagtcttt
     2401 catacctacg atcgatctct ttgactcgca cccataaatt cataatagtt tgtcggcgtc
     2461 tttggacgtt tcgaagatga gctacgcatt ctcttttttt atagacaaaa aagatagaga
     2521 cgaacatgga gcttccaaat ttggatattt gatccaattt tggaagctcc atttatttcg
     2581 tgtctgtctt tttatttttt ccagctttcg actaattctt ggttctcttt gatccagtct
     2641 tttgctgctt gctgtggatc ttttccattg ttgatttcca acataacttt ttccatatct
     2701 ttttctgacc aatggaaatt gtctaatact ttgtaagctt ctggctggtc ttctttcaat
     2761 ccttttcgaa ccatcgtatg gatctgctct tcgcttccca ttccgttttc aggatcttct
     2821 aagtatttca gatcatattt ggcaaacatc caatgaggtg tccatcctgt aactacaatt
     2881 ggttcgtgtt ttttgatcgc ttgacctagt gcaacggtca ttgctcctga agaagatgtc
     2941 tctactttcc agtctttcaa gttgtcatat ttttggatcg tgttttcagc agccgtaact
     3001 acaccagctc ctggctcgat tcctatgatt ttcttacctg cctgatcggt taactcatcg
     3061 atcgaattta catccatgta ggctggcact gctaaaccaa cttttgctcc tgtcaaattg
     3121 gcacctaggt cttcgacttg atctttatat tgtgcatatt gtgaaccatg agttttaggt
     3181 agccaagctg aaaccatggc atctgactcg ccatttgaaa cagatttcca catgattgaa
     3241 ttatctaatg gcgtcatcgt tacatcatag cccatttgtt tcaatacttc accgactaca
     3301 tttgttgaag caacttctgt atcccattca acataagata agttgatacg ttttgtttct
     3361 ttggcagatg aaaaactccc tataccaatc gcacctaaga tcactactac tacagcacca
     3421 atgatcagtc cttgacgttt tttacttttc tgtgttgttg ctcctgcagc ttttttattg
     3481 tttggctggt tcaaatgctg tgtaaaacgg tcaatgatga ttgctaagat aactaatgca
     3541 accccgttga caaatccatt cccgacttgt gctctttgca atgctgataa tacgccgcgt
     3601 cctaagccgg gtgcaccgat catagaggca ataacgacca ttgaaagtga cagcattgtt
     3661 gtttgattga tccctgccat gatcgtactt ttagctaaag gcaattctag tttgaatagt
     3721 ttttgacgac ttgtgctccc gaatgaatca gaagcttcta ctaattcttt aggaacttgt
     3781 cgaatcccta aattcgtaaa acgaacggtt ggtggcagag caaagatgac tgaggcaaat
     3841 acccctggaa ccatcccgat accgaaaaat gcaactgctg gaatcaaata aacgaatcct
     3901 ggcattgtct gcataaagtc aaggatcggt ttgataatac tttgggcttt actgcttttt
     3961 gccatcaaga tccctaatgg taccccaatg atgatcgaga tcacactaga taaaagaaca
     4021 agtgtgacag tattcatcaa atcattccat agaccttggt tataaataaa ccatagaccg
     4081 attaatgtga acaacgtaag tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc
     4141 aataacaaga taaacaaagg agctggaatc actaacagta aatttgtaat ccccgacatc
     4201 actgcctgtc caatcgtttg taaaaatgaa aataggccag aaaaagttgt cgtaaaccat
     4261 tctgtgatat tttctaccca gtttgccact ggtaattgat agttcaatag attattcatg
     4321 ttcactcacc tcgcttgttt ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc
     4381 ttttaatttt ccatcctcta caactgcaag tggagtcggt gagtcataaa taatattgaa
     4441 aatatcactt accaccatgt ctttcccaat caccgtcaca ttttcatcta ggaagtcgat
     4501 caatggacgt ttttgcttcc tagcttctaa cgcttgatct gcagtgatga tccctttcag
     4561 gtgtctcttt ctgtcaacag ccatcagcat gctgacttct tcattacgca tccttgtcaa
     4621 tgcaacgttt ggtccatcag attcgatatt cgtcgtcaat gcaggtacca tgatgttttg
     4681 agccgtaagc actttggaac gatcgacttc ttccacaaat tcacgtacat aatcatttgc
     4741 tggattggtc aatatttctt cacctgttcc gatctgcatg atttcgccgt ctttcatcaa
     4801 ggcgattctg tccccaatcc gtaaagcttc attcaaatca tgggtaatga aaataatcgt
     4861 tttttgaaca ttggcttgca ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg
     4921 atcaagagcc gagaacgctt cgtccatcag caagatctca ggatcatttg ctaaagctct
     4981 ggctaggcca acccgttgtt gcatcccacc agataactga ctaggatatt ggtctttaaa
     5041 tgaaagcaaa ctagaatttt ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg
     5101 tacaccacgg acttctaacc cgtattctgt attttccaag atcgttcgat gtggaaacag
     5161 accgaagttt tggaaaacca tattgatttt atgacgacgg acttctcgca attcttcttt
     5221 gcttaacttc gatacatctt ctccatcaat atagatactg cccgaagttg gctcaatcag
     5281 acgattcaat agacggatca atgtggattt cccactcccg gaaagcccca tgatcacgaa
     5341 gatttctcct tcttttacgt caaaattcac gtcatacaca ccaaccgttg caccggtttt
     5401 ctttaaaatc tccgttttcg gctggtgatc attcatcata tctaaagcgg cttgtgtctt
     5461 tttaccaaat atctttgtta aatgttttac ttctactttt accaaacaaa ttctcctttt
     5521 ttatgtaaat tcatttttct ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt
     5581 cactttgtca aaaatgaaag aaatttgctt gtccaaactg aaaaaaactt ggctaaatct
     5641 ttgtaaataa aaggtttagc caagttaata tttttttaat atttcacaca atcattctgc
     5701 aagctgatgc tccgggtaaa aaaagtttgt catacgctgt agtgtatatt catttccaat
     5761 aaaatgaacg atgtcccctg ccgtaaattt agcatagggg ccaggtgaaa caagcagttc
     5821 ttcattatga gaaattgcga taactgtggc gccagtcgcc tgccagatat tcaactcgct
     5881 aattgaacgg tccagatgca ttgctttttc tgataaagat aattcaaacg gaagtaaagg
     5941 attcatctta tgtattcgct tcgtttgatt gaccagctga tccaataatt gagaaaaatg
     6001 attcagctca tcttgttgct tctccacact tgccatgatt tctgcttttg tatcttggat
     6061 ggattgtaca tcttgatatt gcaataggaa atcttttgct ttttccttcg aagcaacaaa
     6121 tgccccactt ccatgccgta cttccatgat tccaagatct accagcacat tgatagcctt
     6181 gcgggtagtc tcaggagaag cattgtacgt actggcaagt gttgagcggg catggatctt
     6241 ctcacccact ttatatcggt tttcaacgat acgttcggct aaatcaacag caatctgctg
     6301 gtagcgcggg cggacgattt ttttctttcc agacattttc tttcctcttt ctttttttac
     6361 tttccctcat cttactatgc tctagcaaaa taagcaaaaa aggggagaat agcctcttgc
     6421 aacgactgat catcgcttga tctgttcctt ttttactggg caagctgttt gacagtcatc
     6481 acaattctta ccggatttta aacgagtata gacaattgcg cctgcacttc caaagattaa
     6541 cacacttaat aagaatgtcg ccatcctatt cctctccttc taatgacaat atagtataaa
     6601 actggttctt ggattttggc tgacggaaca aaacataaac gagtcctatc atcagtaaaa
     6661 tagcaagata cgtccctaac gctacctgtc caccctccaa taagtgtccc aactgataaa
     6721 tcacaaaact aactaaataa gctaaaccgc attgatagcc tatggcgatc caagtccatt
     6781 tcttggcatt catttcccga tggatagccc caattgccgc aaaacaaggc gcacaaagta
     6841 aattgaaggc taacaatgaa taagctgctg cgggagtata tgtgtgttgt agcacttgcc
     6901 agatttctct gccattttcg gatacttccg catgaccaaa caaaatacca aatgtgccaa
     6961 taatattttc tttagcgacc aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc
     7021 aaccaagcgg ctgaaaaaca actgccagga ttcgcccgaa aaaggctaag atactttggt
     7081 cttcagaaac aggttgagca taaaaattat aagaagagct gaaccagatc acgatactag
     7141 aaacaaagat aatcgttcct gcttttttaa caaaagactt gctgcggtca taggtttgtc
     7201 ttaggaccgc tgaccattga ggaaaatgat atgcgggcaa ttccatgata aaaggagctg
     7261 tatctccagc aaacaaacgt gtttttttca agctgattcc agagaaaata attgcagtca
     7321 taccaagaaa ataggcagaa ggagctaccc aagattgatg aggaaaaaag gcccctgcta
     7381 ctaaggcaat aatcggcaac tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca
     7441 aacgacgatc ttgctcgttt tcaatcgtgc gactggccat gacgcctggc actccacatc
     7501 ctgtagcaat cagcatagga atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc
     7561 gatccatgac aaaagcgatc ctcgccatat atccgcagtc ttccagaaag ctcaagcata
     7621 aaaacaatat aatcaattgc ggtagaaatc ctaacactgc tcccacgcct gcgatgatgc
     7681 cgttcaagat cagctcctgc atccatcccg ctacttgcca ttcagctaac aatctgccaa
     7741 catgttctgg taaccatgta ccaaacaatg tgtcattgat ccaatctgtc cccattgttc
     7801 ctactgtttg aatagccaga taatagatca gccacatcac aaatgcgaag atcggcaagg
     7861 ctaaccaacg attcgttgca atctgatcga ttttgtcact catcgataat tggaaagaat
     7921 catttgacac aacacagagt gtacatagtt gggtaataaa ttcataacgt tcattgacca
     7981 gaatcgtctc actatcgtcg tccaatagtt tctctgttaa ctggatcagt tgctcgatct
     8041 ctttttgctg agaaactgct atctcgtaat cctctcttac cttctgatct cgttcaaaat
     8101 atttcattgc ataccagcgt ttcttgccgc tttgttctcc tagtacatct tcaatttcat
     8161 gtaaagctgc ttctaaacgt ggatcatatg tacagggaac aggaatagaa ggccgctttt
     8221 ttgcagactc ctgaatcttt tcgattagtt tttcgattcc ttgctttttc aatacgctga
     8281 ttggtaaaac tggtacacca agaccatagg aaagcttttc tagattgatt ttttttcctt
     8341 cttttttgat gacatccatc atattcagac caatgatcat aggaatgtct aaatccaata
     8401 actgcgtagt taaataaaga tttcgttcta aattggtagc atcaataatg ttgatgatca
     8461 gttcaggctg ttcctgaacc aaataatctc ttgcaatcac ttcttctggt gtataaggag
     8521 acaaagaata gatgccgggt aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc
     8581 cttctttttt ttctactgtt acgcccggcc aattccctac atattgattc gaaccagtca
     8641 agagattgaa caagctagtt ttcccgctgt ttggatttcc gactaacgcg atattcattc
     8701 tttttcctcc ctttgtatca agattagttc ggcctctgac tttcgtaacg tcagttcata
     8761 ccctcgcact gtcagttcga ttggatctcc cagaggggct agttttcgga taactatttt
     8821 tacatttcgc gtcaatccca tatccattaa ccgtcgcttc actgcgcctt gtccgtggat
     8881 tgctacgacg atcccagtct ctcctgcttt taattgatca agagaagtcc agctttcaga
     8941 ggaagcagtc tttttattga caaagatttg acctagtatt tcccgactta ctgccactcg
     9001 atgacttttt accagtaaaa tggcattgtt gcctgataaa ttgacgacga cgattttact
     9061 tccggcaacg atcccgatat tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg
     9121 ttcgacttga taaacgaaat tctgctttgc ctgcgctaag gtctgcatat tgattcctct
     9181 cttgtgaaat aaatcatgta ttaaaatcat tcattatatg ttctaatttt cttacgtgtt
     9241 acgcaaataa atgagaatca ttctcacata gaatactctc ggtaattgag aatgtcaatt
     9301 atacttgtga tattatgagc gaatctttag tattgataga taaaaatgag aaaaaaatat
     9361 ttcttcatag ttttccacaa ttttttctct attaaatttg ctattttccc acacgatact
     9421 tgttttcagc tcttttttca aaaacacaaa acttagtatt ccacagtttc cacaacacaa
     9481 tatgtagtgt ttgttgtttg acttttttac taagagtttt atactatcta ttatagataa
     9541 taagaatgtg ttagaggagg cactcaccat gaacgtaaaa atcttttcta aaaataattg
     9601 tatgcaatgt aagatggtca aacggttttt aacagaaaat caaattgctt ttgaagaagt
     9661 caatatcgat gaacagcctg aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt
     9721 tcctgtcatt acttctgatg cagaaacagt tatcggattc cgtcctgatc agttgcgtaa
     9781 acttgccagc taacatgact aaaagaatca aaacgtgata catggatttg aagggcttga
     9841 gacagcacca gtttttgtcg caaacttcca aatccttttt taaagcttaa aaacaatgca
     9901 atgatttgca atcaagcatc atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt
     9961 aaaaatttac aagatgtcag ttatttcaag ctgaataatg aaatcaatcg gccagtcaat
    10021 ggtcaaatcc ctttgaataa ggataaagaa gctttagttg ccttttttga ggaaaatgtc
    10081 aaaccaaata cgatgacttt tctatctatt atggaaaaaa tcaattactt gatcgatcaa
    10141 gaatatatag aagcagaatt cattcaattg tattcaccat catttattga aaagctatat
    10201 caatttttgg acgagcaaca tttttctttc aaatctttta tggctgctta caaattttat
    10261 tctcaatatg cgttgaaaac caacgacggt tccgcttatc tggaaactta tgaagatcga
    10321 gtagccttca atgcgcttta ctttgcgaac ggggatgaag aattagcact tgctcttgca
    10381 gacgaaatga tccatcaacg atatcagcca gctacacctt ctttcttgaa tgctggacgt
    10441 aaacgtcggg gcgaattggt gtcttgtttc ttgactcaag tcactgacga tatgaactct
    10501 atcgggcgct caatcaactc tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt
    10561 actttatcaa atcttcgtga agccggcgct cctatcaaag gatatgaggg tgctgcaagt
    10621 ggtgtcgttc cagtcatgaa attattcgaa gatagtttca gctattctaa tcaattaggt
    10681 cagcgtcaag gagctggcgt ggtttatttg aacgttttcc atccagacat tattgctttc
    10741 ctttcagcaa aaaaagaaaa tgcagatgaa aagattcgcg taaaaactct ttctcttggc
    10801 gtgatcgtac ctgataaatt ttatgaattg acacgtaaaa acgaagatat gtatttattt
    10861 agtccatata gtgtcgaaaa agaatatggt gtcccattct cttatgtaaa tattacagct
    10921 gagtatgatc atctagttgc aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat
    10981 cttgaaaatg aaatctcaaa attacaacaa gaatctggct atccttacgt aatcaatatt
    11041 gatacagcga accgcgagaa tccaatcgat ggcaagatca tcatgagtaa tttatgttca
    11101 gagattttac aagttcaaac gccttctgtg attaacggca aacaggaata tgaagtatta
    11161 ggaacggata tctcatgtaa cttaggctcg acaaatatcg tgaatttgat gcaaagccct
    11221 aactttggtc aatctgtgcg tacgatgact cgtgcattga catttattac agatgcatct
    11281 gatatcgatg tcgtaccacc gatccaaaac ggaaataagc tgaatcatac gattggatta
    11341 ggtgccatgg gcttacacac ttatcttgca aaagaacaaa tggaatatgg ctcagaggat
    11401 tcattagact ttacaaatat ttacttcatg ctgttgaatt attggacact tgttgaaagc
    11461 aacaatatcg ctcgtgaaag aaaacaagtt ttccataatt ttgaaaaatc agcttatgct
    11521 gacggcactt atttcgaaaa atacgtaact ggagaattcc agccaaaatc agaaaaagtc
    11581 aaagcattgt ttgaaggcat ttttatccca actgctgaag attggaatgc tttgaaacaa
    11641 gcagtaatga aagatggact gtatcatcaa aatcgcttgg cagtagcacc aaatggctca
    11701 atttcatata tcaacgatac gagtgcaagt attcatccta tcactcgcat gatcgaagaa
    11761 cgccaagaga agaaaatcgg taaaatttat tacccagcgc cttatctatc caatgagacg
    11821 attccgtatt acacttctgc ttatgatatg gacatgcgta aagtaatcga tgtttatgca
    11881 atggcacaac aacacgtcga tcaaggcatg agtttgactt tattcatgcg ttctgaaatc
    11941 ccagaaggca tttatgaatg gaagacaaca acgaagcaaa cgacacgtga tttgaatatt
    12001 ttaagacatt acgctttcca caaaggaatc aaatccattt attacgtacg tacctttact
    12061 gatgatgcag aagaaatcgg aagcaatcaa tgtgaaagct gcgtgatcta atatcataga
    12121 tagattcgct tattcatatt ttaattaaag gagcattctc atgtcagaaa catattatga
    12181 agcaataaac tggaatgaaa ttgaagatat cattgataaa tccacttggg agaagctgac
    12241 agagcaattt tggctagata cacgtatccc actttcaaat gacctagatg attggcgtac
    12301 attgtctcaa ttggaaaaag atacagtagg acatgtattt ggcggtttga cactgctaga
    12361 tactgtccaa tctgaaagtg gcatggatca gttgcgtaaa gatgtccgca ctccccatga
    12421 agaagccgtt ttgaacaata ttcaattcat ggaatccgtt catgccaaaa gttattcttc
    12481 tatttttagt acactgaata cgaaaaaaga aatcgaagaa atttttgact ggacaaatac
    12541 gaatccttat ctgcaaaaaa aagctgagcg tatcaatgaa atctacaaac acggtacacc
    12601 gctagaaaag aaaattgcca gcgtcttttt agaaacattc ttgttctatt caggttttta
    12661 tactcctctt tattatttag gaaacaacaa attggctaac gtagcagaga tcatcaaact
    12721 aatcatccga gatgaatctg tacatggaac ctacatcggt tacaaattcc aattaggatt
    12781 caacgagtta cctgaagcag aacaagaaac gttaaaagac tggatgtaca atcttcttta
    12841 tgagctttac gaaaacgaag aacgttatac agaagaatta tacgatccaa tcggctggac
    12901 agaagaagtc aagacattcc tacgctataa tgccaacaaa gcattgatga acctagggat
    12961 ggatccactc tttccagata ctgccaatga tgtcaaccca atcgtaatga acgggatttc
    13021 aacaggcaca agcaaccatg acttcttctc acaagtcgga aacggctatt tgttaggtca
    13081 tgtagaagcg atgaaagacg acgattattt gattggattg gaataaggat ttattttaat
    13141 tcaaaaattt ttcgaaaaat aaaaagagtt tagcgtacag atattttaaa tgtacactga
    13201 actctttttt atgcttattc acttgtttct tccagcactt ttggactgtc taacttttcc
    13261 gaaacatgtt ccattcttct caataaatag attttccgga tatttaatat tatcgtctta
    13321 atcactgcat agacaggtat gcaaatcagc atgcctaata ttcctgcgac acttcctgct
    13381 ccaatcaata ccaagatgat cgtcaaagga tggatattca ttgatttccc aaataacaac
    13441 ggcttgatca aatttccatc taattgttgg atcactaaaa tcgacaacgc catatacaaa
    13501 gcttgaaatg gagagataaa caaaccgaca atgattgctg gtaccgctcc aataaaaggg
    13561 ccgacatacg ggataatatt cgtgattccg caaatgaccg caagcaacaa gctataaggc
    13621 tgatcgaata ttctcattgc caaaaagctc attacaccaa taatcaaagc atctaagatc
    13681 gtactgctga tataggcaga taatgtttcg tttaattctt ttaccgtttg gcgtaactct
    13741 ttacgaatcg ctttcggaaa gaagtgagac aacgcatcaa taaatttgtg cccatccttg
    13801 aacataaata gcaaaataaa tggaacggta aataacaaaa taaaaaattg catcataaat
    13861 gaaatgattt ttgagacact tgcagataaa ctgactaata tgatattgaa caaattacta
    13921 agagataaat ttgctgatgc taattgttct tccaaattga aatttttaaa ctcttcccgc
    13981 tgcgccaatt catttaacca tttgcttgtt tcatctgcat acattggcaa gctctgggtc
    14041 aattgaattg tctgttccat taactgaggc actacattca tgacagttaa aacaactaaa
    14101 atgattacga ccaaaaacgt caataaaaaa ccgaacactc tcgggatccg atgcttttcg
    14161 agaaaaacaa cgattggatc gaacatataa tagataaaac cagcaacaag caatggtaaa
    14221 aaaactgccg aaagcatttt caatattgga ctaaaaatat tcggcatttg taaaataaag
    14281 tacacgccga tgatcgtgag tactagctcc actgtccaga aaaataattt tgattgttta
    14341 aaacgtgatt gcatacatct cctccaaggt cctttgatac ttctattcta tcatttaaca
    14401 aagagttacg cgctgagatt ttcatttgct taaaaaaaac aaaaaaaata aacatatttt
    14461 aatgagtaga cgagtactta aacaaaaaca cggtttggag tagtgacaaa aattatactg
    14521 cagtcccttt ttccaaataa acggtgttca aaagcttgca tcttcgtaat aagcccagcc
    14581 aaacgaaaaa tataaggaac tatttttatt tggctgggct tattacttga tgtaggacat
    14641 ctattttaca acctcagatc atttattgcg caatactgac taaatgcaac tttgtgatat
    14701 ttttgtgtac ggacaactcc tcgatcacat cggcataagt gagccctttt ggaagatcaa
    14761 tcgtatagat atttttgtac actcggtagt catcaacgaa ttttacgtca aaattcacat
    14821 cttcgattac gatatttttc tcttcaaaat aatcggtaag aaaatctttg gtttcttttc
    14881 tatggataaa ttggatttct aactttttag tcgtaggtac atggatcacg cgtttcacta
    14941 ctgtgagtgc aatagcaatc ccgataaaac tgatgatcgc aatggaatag taacccatgc
    15001 cgatggcgat ccctaatcca gcaattgccc ataaagaagc ggcagtcgta agtcccgtca
    15061 ccgattgttt cgtgacgatg atcgttcccg cgcctaagaa accgacacca ctcaccactt
    15121 gagcaatcag ccttgcttca tctgaacgaa tcgttccagc aagctgtgga tactctaaag
    15181 catctcttaa tgcaccacta gcgatctcaa cttggatcaa tgcaatgata catgctccca
    15241 tacaaactaa aatatgcgta cgcatacctg ctggacgatt tttatattgt cgttcaaaac
    15301 cgattgctcc gccaaatagc atagcgatcc ctaaacgtaa aataatctct ggaattgtca
    15361 ataccatatc attcatctaa acaccctcca gtccaatata tcatgtctga aaggagcaga
    15421 caaaaaaagt gcaattttta cctcttacga aaaaaggact gtgacaaaat ttttgtcata
    15481 gtccttttta tgaataaacg gtgttcaatc agctgaccct cggtattact caacaaacgg
    15541 caaaatatgg ggagctattt ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt
    15601 tcacaacctc tttagcctac tattgaaaat tacaacttac ttgctgcttc ttcatcgatg
    15661 atcacaacaa cattgtcgtg attttgcaat acacttgctg gcaaatgatt cgttacaggt
    15721 ccttcaatca tccctttgat tgcatcagct ttgctttcgc caaatgccat caagacgatt
    15781 tctttacttt tcataattga accgattccc attgaaacag cttttgttgg tacatcttct
    15841 gctttatcga agaatcgttt gtttgcattg attgttgatt ctgttaagtc aactactgaa
    15901 gtcgttccgt caaatgaagc accaggttca ttgaaaccga tatgtccatt ttgcccgatt
    15961 cctaagattt ggatatcgat tggatgtgca tcgatgattt gatcatactt tttgcatgct
    16021 gcttccagat cttctgcttt tccatctggt acaaacgttt ctttgaatgg ttttttatca
    16081 aataattgga catccatgaa atgacggtag ctttgttcat ctgaaccgcc tagtccaacg
    16141 tactcatcta agtttacaga agtcatttca ctgaaatcca aatcgcttga agtcatttct
    16201 ttatataaag taactggtgt gcttcctgtt gccaatccta atacttttgc acctttgttc
    16261 ataccttctt tgatgatttc aaatgctttt tttccgcctt cttcagcgtt ttttacacga
    16321 atgatttcca tagtgattgc tcctttatat tttggtatag cccaattata gcattctttt
    16381 tttcttttta caataattag tatagaccaa attgcatttt tttcattttc ttgacaataa
    16441 ataggaatcc gtgttaggtt tcataagatg gttcaatcaa acaatccatg ttgcatgaaa
    16501 accaagcaag tgattcactg tggacgcttt atgtctgcag tttttttatg aaaaaggagt
    16561 tattatgagt atactgacat taaacaacat tacccagcaa tttggcgaca aaatcctcta
    16621 tgaaggtgtc gatctgcaat taaatgctgg cgaacatctt gggctgattg gacaaaacgg
    16681 agccggtaaa agtacattga ttaaaatcat cactggtgag atcttaccag atgacggaca
    16741 gatcaattgg caaaaaaata ttcacaaagg ttacctcgat caatatgtgg aagtcgatga
    16801 aacattaaca atcgaagaat ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga
    16861 aaaaattgct tttctttacc aaacatatag cgagaccttc gatgattcct tgctggaaaa
    16921 agctgggaaa ctccaaacgg aattagacca aggagtgttt tatcaaatcg acaccttagt
    16981 tgctgagatg agcagcgggt tggggatcga tgtcttaggt ttagattcac tgttgaaaaa
    17041 tctaagtggt ggacaacgtt caaaggtgat tttagcaaaa ctacttttag aaaacccaga
    17101 tgttctgatc ttagatgaac caaccaatca cttagatgat cagcatatcc aatggctgac
    17161 gcagtttctt caggattttg acggtgcgta tatcgtgatt tcccatgacc aagaatttct
    17221 tgatcagatc accacccata tcgcagacat cgagtttgga aagattacta agtatactgg
    17281 ccatctaaaa caagcattga aacaaaagga acaaaatcgc gaaagctatt tgcgccaata
    17341 tcatgctcaa caaaaacaaa tcgaaaagac cgaagcttat attcgaaaat acaaagccgg
    17401 ttcccgatca acgatggcaa aaagccgaca aaaacaatta gataaaatcg aacggctgac
    17461 gcctccagct tcagcaagta aagctgtctt tgattttcca ttctcgccta ttgtcacgac
    17521 attagcagtg gaaacaactg atttagtcat cggatatgat cgaccgctac ttgcacccat
    17581 caatttgacc atccgctttg gtgaaaatat tgccattcga ggcttcaacg ggattggaaa
    17641 atctacttta ttaaaaacac tgatcggtga gattgaaaaa ataagtggcg atttccactt
    17701 cccagataac acaaaaatca attacttttc acaagatctg tactgggaaa atccattaga
    17761 aacaccacta caatatttaa gcagtcaatt tccaaaagcg actatcaaag aattacgtcg
    17821 ccaactagct aaagcagggt tagttaatca attagcaagt gaacctctca ctacattaag
    17881 tggtggggaa caaactaagg tgaaacttgc acagttgaca atgaaccaag gtaatttact
    17941 gattctcgat gaaccgacga accatatcga tcaagaaacg aaagaaagct tacaagacgg
    18001 gatacagaaa tttccaggaa cagtaatcat cgtctctcat gaacaagaat tttatcaaga
    18061 tcttgtgcaa cgagtcattg agattgaacc aaaataaacg tttgattgaa tagttgaaaa
    18121 aaaggagctg tgacaaaagt tttgtcacaa ctccttcttc tgaataaatg gtggccaatt
    18181 agctgacctt cggtattagc tcaaaaaacg gcaaaatatg aggagctatt tttatttggc
    18241 tattcttatt acttgatgca gaacagcttt tcacaacctc ttttcgtcta ttttttattt
    18301 tcgagatttc tctttccttt aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa
    18361 acaagcccgc taaccgttac acctaaccag ccatactgct gccacattgt cgtccctagc
    18421 aatgaaccag ccgcacctcc aataaagtac ataaacataa atacagtatt atttcggttg
    18481 cttgctgctt cacctagccc ttgtactcgt gtttgattgg cgacttgacc aaattgattc
    18541 ccgacgtcta ataaaataat agctaaaagc aaaatggcca aatggcttcc gccaaccaat
    18601 aaagcaataa agcttattgt ttgcataaca agtcccatat acacgatttt tctttcagaa
    18661 taagaatctg ccagtcgtcc cacgataggt gcagcaactg ctccagacaa accaaaaatc
    18721 gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg gactgctgat ataaaaaatc
    18781 aacgtagacc aaaagatcga aaatgttcca aacatgaaaa atccactgac tgccgcttct
    18841 cttaatagac gttgagaagc aactaaatgc ggcaaacttt taagtgaatc aaggtatgaa
    18901 acagctgtac tactttgctg gacagaacgc ggcattttcc agtaaagcag aagtgtaaga
    18961 attccgactg cacataaagc aaataaatag atcatccgcc aagaaaaaag actggcaaca
    19021 ataccagata ccgtccgaga aagcaaaatc cccacgagta aaccgctaag caatgtaccc
    19081 atgaccttcc ctcttgcttt tggtcccgct agtaccgcac cgtaaggaat gatgatttgc
    19141 ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg caaataacgt aaacgaaggc
    19201 gcaaaaaaag cggccaacag agagacggca gataacgctg ccattcgaat gatcaatttt
    19261 ggacgatcca ccacgtcccc taaaggaact aaaaacagta accctaacgc atagcctaat
    19321 tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa cagataaact agctgctact
    19381 tttgttccaa tcggctggat gtaatacata ttggcaacaa caacgccaca agtgattgat
    19441 aatagtaaaa taagcgaagt tgttaaaggt tttttttcat tactcttcat tgatttcccc
    19501 catttcctat cttagtttac aacttatgga aaagattgga aatagaaaaa tacaactatt
    19561 cgccacactt attatttgtg cttttgtttt ccttgttttt tcttgcgatc tttataaatg
    19621 aagacaacta accctactag cacagtaaca cttgcaacca acgtgactgc agttttgtta
    19681 tatccgtgaa acagagcttc tccaccacac gcatacaaca cagacgaagg gatgactcca
    19741 atcacgatcg acaatatgat ttgctgtaga ggcaatttca tcgcatctgc agcaaaattg
    19801 accatagaag atggaataac cggtatcata tagcctaaca ttacacctat ttttggatgt
    19861 ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg ttttcttatc taaaaatttt
    19921 aagcgatgca taaggaaaat tgcagtgatg ttaccaagag cattacccaa aatgttgatg
    19981 atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga ctccaactac tgaagtagga
    20041 atacctggaa cagcacacat caccgctgtc aatacgatca agagtatcga tgcaaaagtc
    20101 ccatgcgaac gaatatgtgc gagtagctct gccttactcg ctctcggatt taaaaataat
    20161 tggatgtctg tctgatattc taaatacaac cgatagccca ctaccagtat cagaatgatt
    20221 cccaacgaga tcaatatcaa tcggattatt ttctttttca tatttcctcc tgaaaaaaat
    20281 ctatattcat taaataatag tttaacataa aaacaaaaaa gcaccgaaat ccaacatagg
    20341 atctcagtgc ttttaaatag taaaaattat ttaacagtta cagatgcgcc aacttcttca
    20401 agtttagctt tcaattcttc agcttcttct ttagatacgc cttctttaac tggtgcagga
    20461 gcgccatcaa ctacagcttt agcttctttc aagcctaaac cagttgcttc acgaactgct
    20521 ttgataactt taactttttg gtctccagct gcagttaatt ctacagtgaa ttctgtttgt
    20581 tcttcagcag cagcagcgcc accagcagca gctacagcta caggagcagc agcagatacg
    20641 ccaaattctt cttcaatagc tttaactagg tcgttaagtt ctaggatagt tgcttctttt
    20701 aactcagcaa cgatgttttc aatgttcaat gccattataa tttcctccgt ttaattgttc
    20761 ttttttattt aatgctttat gcggcaatta agctgcatct ccatcttctt tgtctgccac
    20821 tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt agtacagata gcaacataga
    20881 tagaagtcct tcgcgatttg gcaattttgc caatgctgtg atttcttcca atgaagagac
    20941 tttaccttcg atgataccgc ctttgatttc taatgctttt gcttctttag caaattcgtc
    21001 catgattttc gctggtgcga ctacatcttc gttactgaat gcaacagctg taggtccagt
    21061 gaatacttcg tccatgcctt caaggccagc ttttttagct gcacgtgaaa gaatagaatt
    21121 tttgataact ttcatttcta cgttagcatc acgaagttgt ttacgtaaac gtgttacttc
    21181 atcaactgtt aatccgcggt agtcaacgac tactactgat gcagctgctg taaatttttc
    21241 gcttacttca tcaacgattg ctgctttttt tgcgataatt gcttcactca tttttgagtt
    21301 tcacctcctg aattttgatg gaagagtttt ttgcactgaa aaggcaagtc acttttatag
    21361 ttactttgct caaccagtat tattcatcac ctattcactt cgttcatagt gattctactc
    21421 gctttgctcg aaaggaaact cctttcgctt tgctcaacca gtattgttca tcatctcttc
    21481 acttcgttca tagtgattca caataaaaaa ctctatgcca ccgcagacat agagggacta
    21541 ttgaatatat tcaataaacg tcctcggtag gaaattaagg cttgatgcca cctactgtct
    21601 tcggtacaag ttaattatta acctcaacta gcttagcatg ctaaactagt tgagtcaaga
    21661 attatttttc agttggatca atcaacattc taatatttaa ttagaaagaa gcttggtcaa
    21721 catggatacc aggtccaaat gttgttgtga tcgttacatt tttcatgtat tgacctttag
    21781 ctgctgatgg tttagctttt agcaatacat catggattgc tgcaaagttt tcaactaatt
    21841 tttcatcgtc aaatgaaact tttccgattg gtacatggat atttccagct ttgtccacac
    21901 ggtaagttac tttaccagct ttcacttcgt ttactgcttt tgttacgtcc attgtaactg
    21961 taccagtttt agggtttggc attaagcctt ttggtcctaa tactcgtcct agacgaccta
    22021 cagtagccat catatcaggt gttgcaacaa ctacatcaaa gtcaaaccat ccgttttgga
    22081 ttttttgaac catgtcgtcg tcacctacga aatctgcacc agctgcttca gcttcttttg
    22141 ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt accagttccg tttggtaata
    22201 caacagcacc acggatttgt tggtctgctt ttttagggtc tacgttcaaa cggtatgcaa
    22261 cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt tgctaaagct actgcttcgg
    22321 caacaggata aactttactt gaatcaactt tttttaatgc atcttgcatt tttttgctct
    22381 ttttagccat tttggttcct ccttgattgt ggttataacg gttgtacctc ccacgtgttc
    22441 tatatctttc aatatagagc caactgagaa gctgctgtct tagggtgtat agattattct
    22501 acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc gcattgctgc ttcaacgtta
    22561 gctgcgttta ggtcttccat ttttaattca gcgatttctc ttacttgatc gctagaaact
    22621 ttcgcaactt tatttttgtt tggttcgcct gaaccttttt cgattttagc tgcttttttc
    22681 aatagaacag cagctggcgg tgtttttgta acgaatgtga atgaacggtc ttcgtataca
    22741 gaaataacaa ccggaatgat caaacctgct tgatcagctg tacgagcgtt gaattctttt
    22801 gtgaatccca tgatattgat acccgcttga cctagtgcag gacctactgg tggagctgga
    22861 gttgctttac ctgcaggaat ttgcaattta acgatttttt ctactttctt tgccacgaga
    22921 catacctcct taagtccgtg atgtggttaa ttggagatgc atatttctcc tcccacgttt
    22981 tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac aatgcacact ggtacagtat
    23041 agcaaattta tttgtttttt caagattttt ttctgctatt tttacatttt gcttgaataa
    23101 acgctaaatt tattctaaaa aaacaacaat ttttcgttct catttcttct tttatgttac
    23161 acttgcgcta gcatggttca acagatgaaa gcaaacaata acacggtttt ttagaccctt
    23221 gtacaaagat tcctacataa caaaatgagg tggagaaaat gaaagattta aggtatagaa
    23281 gtgtctttga tattattgga cctgtgatga ttggtccaag tagctctcac actgccggag
    23341 ctgctagaat cgggaaaatc atacgtagta tatttggcga acagcctgat tctgtagata
    23401 tttatctata tgaatcattt gcaaaaacct atcgggggca cggaacagat attgcattag
    23461 ttggcggcct gttagggatg gaacccgatg atgaacgttt agctgattct ttgaaattgg
    23521 catatgaagc cggtatggaa gtcttgtttg ttcctaagag tgaaaaagcc gaacatccaa
    23581 actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct ttcagtcact ggcatttcga
    23641 tcggcggtgg caatatccag atttctgaat tgaatggatt caaaatttct ttaagcatgg
    23701 gtactcctac cttgatcgtt gtccaccaag atgttccagg tatgattgct aaagtgacta
    23761 gtatactttc tgagaccaat acgaatatcg gcactatgac agtgacacgg gaatcaaaag
    23821 gtgaaaatgc cattatgata attgaagtag atgatcctca agtagaagaa acggtaaaaa
    23881 gactcaaaca gctgccaaat atcgacagcg taaattattt cgattaagga gctaaaaaaa
    23941 tgtttttatc gattgaagaa ttagtagatc aagcaaaagc ttatccaagt gtagcagaat
    24001 tgatgattgc agttgaaatg gaaatgagtg gccgttcccg tagtcacatc atcgagatca
    24061 tggaaaaaaa cctctcagta atggaacaat caatcaacga gggaaaagaa ggtgttacct
    24121 ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga tgattattta aatgaggcag
    24181 actttttaag tggagaaaca atcttaaaag ctgtacgcaa tgctgtagct gttaatgaag
    24241 tcaatgcaaa aatggggttg atttgtgcta caccgactgc cggaagcgcg ggtgttgttc
    24301 ctggtgtgct gatggctgtc cgagatcggc ttgatttgac acatgaacag caattggaat
    24361 tcttatttac cgctggcgct ttcgggttag tcatcgcaaa caacgcttct atcagtggtg
    24421 cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc aatggcagca gcagctttgg
    24481 tttgtgcaaa aaatgggact cctcatcaag cagcacaagc agtagccatt acattgaaaa
    24541 atatgatggg actgatttgc gatcccgttg caggcttagt tgaagtcccg tgtgtcaaac
    24601 gtaatgcttt aggctcttct caagctttta tctctgctga tatggcatta gcgggtatcg
    24661 aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta ccaagtcgga cgtcaaatgc
    24721 cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt tacgccaact gctaaacgac
    24781 ttgcggaaga gattttaaat aagagggcgt gataaagctc cttaatttcg agtattaaga
    24841 gattagttat cttcataaca aaaaacgaag agaggactaa ttgcccttct cttcgttttt
    24901 tattaatcca tcaatttcgt attttttact tcatcgatcg tttttgttcc tgcaagttgc
    24961 atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag atgttactcc ttctgcccca
    25021 cctagattca acccataaat cactggtcgg ccaatcgcaa ctaagtcagc accacttgcc
    25081 agagctttga agacatgctc tcctcgacgg acaccagagt cgaaaatcac tggtacacgt
    25141 ttgttgacta cttcggcaat ttttggcaat acttcaaagg acgcaggacc tccatctagt
    25201 tgacgtccac catgattgga tacccagata ccatctgccc ctgcagagat agcgattact
    25261 gcatcttctg gcgattgtat tccttttacg ataactggaa gatgggtgat ttctttgatt
    25321 gttttgatgt catctggtgt caagccttgt tttgcagctg cataaatttc tgcgattcct
    25381 tttccttcgc cattaccact tgcactttgt tcgctatacg cagctaagtt aggcattggt
    25441 aatggaaatt ggaattgatt gatgacgtct tcttcacggt aaccaccgag cgttgaatct
    25501 gccgttaaaa tgatagcttt agcccctgct tctactgctt tgttcaaaat gaactcattg
    25561 aatctgtcgt ctttactcat atataattgg aaaaactgcg gtgcgcctgg cgctgcatcg
    25621 gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg aaaagattga tccagcagca
    25681 gctactcctt tagctgtatc agcttcccct ttttcatgtg ccaatccttg ggccgctgat
    25741 ggcgcttgga taatcggtgt atctaattca attccgaaaa cagatgtatg caaatcggct
    25801 gagtcgattc cgcgtaatat ccgtggcatg attttctttg tattaaaaga agtcgtattt
    25861 tctttcatcg tccactcatc ttcagaacca ccgcgaatat agccaaaagc tcctgcttcc
    25921 atgcgctctt ttacacgacc ttctagttag gcaatgttta caatatcgat tggatgttct
    25981 tcattacttg tatgataagt catagtagat ccttctttca tcttcttgat tacatataaa
    26041 tcatactaaa cttacaaaaa gtaagcaaac atttactctc agatactttt atcgctattc
    26101 tttccaaata aatcgactta caagctgatc tacttcttgg ttttcatgaa gcatactatg
    26161 agaggcattt tcacctgtca ctactttttc ctctatttca tttccgcgtt gcttcaacaa
    26221 cgcatagacc gctaatgcac tgtttaacgg tactgttcca tcactcaagt ctgtttcatc
    26281 tagttgacca gcaatcaaga aaaatcgagt ggttatagga atattaccga tcatttgctg
    26341 gtaatcttga tagcggttag aaacgacagc aggccccttt gctaactcat ctgtcagaga
    26401 ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca attgctgtta atgtctgaac
    26461 ttgtggcaat gacgtatcct gtccatacgt tcctaaatag cgtagtgcac taactcctcc
    26521 catggaatgc cccactatgt tgactttatc tatgccgtag ttttctttca gatacaaaag
    26581 acaagttttg atccattctg cttgattcca ctcgttattt ttattatctt caaacaatac
    26641 ttggaccatc ggattgtctg cttttccaga caaatctcct gttgcttgta tttgtccatc
    26701 tgttcctact gttaaaacca attcttttac tgcttgctga tttttttcca aacgccgcaa
    26761 catcccttta aaggaatttt ctgtcccgct gtagccgtgg aaaaaaagtg ttggaatagt
    26821 ggttttttct atcactttac tggattctgc cgttttttct ttatcagaaa cttcagtagt
    26881 atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa ctgataagga ctaaagctgc
    26941 tgcagttgca cgtttgcata aatttttgat tggttctgtc atacacctct tcctttccat
    27001 atagaaaaaa gaaagacttg aaaggccaat catatgatcg acatttcaag tctctttatt
    27061 aatcgatttt atctacttga tcgaaatcaa gttctgtact ggtttcacgt ccaaacatat
    27121 cgatgttgac tttcaatttt tctttttctc cgtcaacttc tgttacttgt ccttctaatc
    27181 cagcaaatgc gccttcgatg attttcacga cttcgccaac ttctacttct agatcatttt
    27241 gtcgcaggct cataccgata gaacgcaaga tatgattgac ttcttctgga agtaatggtg
    27301 ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt cacaccaggt gtgttgcgga
    27361 caacatacca tgaagcgtct gtcatgatca tttcaacaag tacgtatcct gggaatgttt
    27421 tatgaacgat ttctttttct ttcccatttt tgacttcagt ttctttttct tcaggaacaa
    27481 cgacacggaa aataaagtcg gccattttca tactttgcgc acgagattcg atatttgctt
    27541 ttactttgtt ttcatatcct gaatatgtat gcaagacata ccagtttttt tcaaaagatt
    27601 ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc tcagtacacc cgaagttttt
    27661 ctttcgcttc attatagcac taaaagtacg ttgtggctag cgagttattt taaaatccag
    27721 ccaaataagc tttggatggc tgtatccatt acgtaaaaca tcaatccgaa aataatagaa
    27781 gtttcgatga ctactaacgt atctttacgg agttgttttc tgcttggcca agttacttgt
    27841 ttgatttcat cttttacgct ttttagaaat ttcatgagac aacctccctt gctactgttt
    27901 atttcgtttc tttatgtaat gtatattgat tacaatattt acaaaactta ttgatttcca
    27961 aacgttcgcc gctttttcct tcactgactg attttgtgta gttgcgagac ccacaaacgg
    28021 aacaagcgag tgctgctttt ttctttgcca tttacttctg cccccttgct gagaatcaga
    28081 cttatacttc ataacagtat cacgtaactt tttacttgtc aatttgtgaa ataaactatc
    28141 tattgttttg gataaaatta tgatatgatt aatttgaaat taagctaaaa tgagacaatt
    28201 ggaggtatag cgatgctatt aaaaacactt gtatataaga aaaaagatct gacaaccgtt
    28261 acagagacta gtacattaga agaagcatta aaaattttag aaacttccgg ataccgttgt
    28321 gtgccgatct tagattcttc aggtaatatt ttccgtggaa acatttacaa aatgcatatc
    28381 tatcgtcata aagcaaatgg cggtgatatg acgcttcctg tcactcatct tttgaaaaac
    28441 gctacaaagt tcatttacct ggatacttct ttcttcaaag tattctttac gatcaaagag
    28501 cttccttata ttgctgtcct tgacagtgaa aatcattttt atggtatttt gacacatagt
    28561 acgttgctta atatgctctc tcaatcttgg agcgtagaac aaggaagcta tgtactgaca
    28621 attgcttcca caggaaagca aggagatttg gctgctattg caaaaatcat ttcaaaacac
    28681 agcagcattg ccagctgtat tactcttgat atcgaaaaag acgaatacat tcgacgtaca
    28741 ttgatcacgc taccggctaa cactgaagcg aaagtatgtg aaacgatcgt caaccactta
    28801 gagaaaaaga attataaagt ggtagaaatt gaaaatttgc aaaaccaaga agcataatca
    28861 aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt cgctttttat aattagacac
    28921 gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa tggtgctgaa acagccatga
    28981 cgataaactc acttaatgca agcgtgccat aggttgtcca aaatgcttgg ccacctgttg
    29041 gaactaacat ataagcaatc agtcccatgc tgatggtaaa gaagatcgtg ttcaatacta
    29101 aacgcatctt tacatttttc actttgtttt gcaataaagc tgtcaggcct aaagaaagca
    29161 atgtttggcc gccaccaaaa accacatcca tgataccaaa accaaagaag gcattataga
    29221 cgatgacccc gcctaaaact ccccaaagca atttacggtt aaagacaact aaatgattca
    29281 aactttctga aatacgaaac tggatcggcc ctgaggatac aggcgcaact aaaatagtta
    29341 acgctagata aagagccatg atgatgccat ttacaacgat tacggataac cgttgtctgc
    29401 ttttcgtttt ttgcattttc tctcctcttt tctcctagtt tttttaacgc gggatggttg
    29461 atgaaccgcg gttatgtgaa acaaacaata attataacag acttttcatt tatttcaatc
    29521 cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg taaacgtttg ctaaaaatcg
    29581 tgggatatgg tataaagtaa gtgtaagggg tcataagaat tcccagtaaa gcagacgaag
    29641 taggatctgc aagaacagaa ttgttgtgaa acttaaataa ataaaaaagg atgtgggaat
    29701 ttgagcaacc tcttactaga gaacggtcta gcaagcagct gaagatctaa gtggacatgc
    29761 cgaaccttgg aacttctcct tacgtaagta atggatcatg cgcttgctag gcttttttgt
    29821 gtttttcggt cttcggacct ttttcatttt cctagacttc tgctataata accaagaatt
    29881 aattttgtaa cagatttttt tgaaaaaaca agtttctttc attcaaaaaa tagaaaaagc
    29941 ttgctaatta ttggttaacc agcaaacagg agtattaaaa tatcttgttt tattgaatag
    30001 caggaggaaa tcatgaaatt ccattatcat atattgatca atccatcggc aggcagcgga
    30061 aatggctata aagtagcaga aagaatttta ccggtattaa aaaataagca tattgactat
    30121 acacttcatt atagtgaata caaaggacac gatgctgaga ttgctgaaac tttggcaaaa
    30181 gaaacattga ttccttggat agaagaagaa cagaaaacaa tcgatacttt cccccttttg
    30241 atcatcgtag gaggtgacgg caccttacat caggtacttg atactttcta tcagatggaa
    30301 gtagaattcc cagttgctta tattccagca ggttctggag atgattttgc tcgaggggca
    30361 ggccttccaa aaaatcccaa aaaaggactt cagctgattt tagcagctca atcaccagaa
    30421 aaagtccaca taatggctta tgaagaaaaa ataagtgaaa aaaaaggaat agctgttaat
    30481 aatttcggta taggattaga tgcagctatc gtccatgcga caaatcattc ttccacaaaa
    30541 aagcgattga acaaatacaa cttaggatca ttttcgtatt tattctctat tctccgtgct
    30601 ttattcactc aaaaaggatt tccaattctg gtagatgcag gaggaaaaca attaagtttt
    30661 tcaaacgcat ttttatgtac agcaacgaag catccttttt ttggcggagg aatcgcgatt
    30721 gttccaactg ctgatgcgtc taaaccagtc attgattttg tcgtggtcga acgaatcaat
    30781 ttctttaaaa tcttgtggct cattctttta cttatacaga aaaaacaatt gaaatcgaaa
    30841 tatttccatc actacacaac aaacagatta cgtatcgttt caacaacacc gcaacatggt
    30901 caagaagatg gagaagaaat ggaaaaacga ccttttgata tcacaataac cacaactaaa
    30961 cagttgtttt ggtgttaata aaagaggttt tgtcagaagc tacatctgga acaccgttta
    31021 ttcggaaaaa aggactgtga cacgactttt gtcacagtcc tttttttact atgcagttaa
    31081 ttctttgccc aattgggttg ccatcgatag tttctttctt tacccaaaat aaattggatc
    31141 attgaaaaca ccgcagcaaa agcaactaag acacaaccat tgaaccaaaa agattctgca
    31201 aatgtttgat tgtaactgag tgtttcactg atcaagttat tattttgaac aaaccaatat
    31261 gttggtgtga atgccgctat tttgctaacc atttctggca agtattctgc tggtacaaac
    31321 accccaccga taaagcaact gcctgtgata aaaatatctt tgatcccacc gctggtttct
    31381 gagtttttta ccaatgaagt gatacaagcc gaaaagctaa tgatcggcaa taaaaacaag
    31441 aaagtattta aaatatgata ccataccgtt atatcccacg aggtgtggat cactaacaga
    31501 accattagaa gtaagaagcc actgaacaac agcagcgagt aactaatcaa actaaaggtt
    31561 actttgcgat tcaagtttcg tttggtaatg ggcgcactct ggtttcgttt actgatctct
    31621 ttgcgattaa atgctagatt gatgacgctg aaaccgctaa aaatggatag aaacaaccca
    31681 taagacaaga aattaaaggt tgtgcccgtc aacagctttt tcatccgctg actatatccc
    31741 gcacttagct caacttctcc agtaaccgag agtgccattt gtgtctgttc catagctgca
    31801 tcgattgttt tatcttcttc ttgaaacaga atcagtgttt gcaaaaaggt gttcacttgc
    31861 tgtgtaacaa ctgttttaga aaaggcatct gggcgactct gtatcttgat cgtcggcatc
    31921 tttcctactt cgacttgttc ggtaaagtct gcaggcagat gtaagataat gtttacacga
    31981 ttaaaataaa gagcatcatc gatttctttt tgtgaggttc catccaatgt aacgactgtt
    32041 tccttgtcat tcaaatattt acgaaggcca tcgactacag gcgactcttc ttcactaatc
    32101 aaggcgattt tcgcatcttg cagagaggta ttttcaacat caatattttg cgcaaaaaag
    32161 aatgtggcaa caacagtgat aattacacct atcagtaaat tcgatttatt tgctttaagc
    32221 aatgtccaaa tcgttttata aagcgtcata acgcacccct ctttccatcc aagcatttcc
    32281 taacgcaaaa aggaaaataa atccgatcaa ccatagaaga ttggtataaa aactagccaa
    32341 atcagtgtag aaataaagtt tatacaaact ttcgctgatc aagttgacga gatttaattg
    32401 accaacaatc ggaatatgaa gatcaatcca atacttcact tgttccgacc ccatcatacc
    32461 agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg ctatcctttt gcaccagatt
    32521 catttttggt atcaaattac caaccaatgt acctaacaaa atcgcacaca acgaccccag
    32581 agcggtaaca atcatcaacc attgccaacg ttcaccaaag tctacttgat agataaaacg
    32641 aaagacagct aacataatat aaatctctgt caaaacaatc gtaaagcttg ctgccaaatt
    32701 agcgattgaa actaacgatt gctttctagg aatcaaggac aagcgaatac catttgacga
    32761 ttgatttgct tgctgatcct gtacattgcg gaccccccac atcgtcccgt agagaatcgc
    32821 cattccaatc agagtgaaga aataaaaact tttgaaactc atattacgac ctgcatgaac
    32881 ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg ggcgcgagat caacgttctc
    32941 cacttctgcc actttactcg aaattatacc aatacgctgt aaaaattgat ccatgacgtt
    33001 tttcatcaac gtctgactca ttcctgcttg acccacatgg agcgaaacag atgaaccatc
    33061 aatgagataa aaaccagcca aatcatctgc atttagcttt tcattcgctt ctacttccga
    33121 tagttcctgt acttcaaata atggtttatc attgacttcg acttgttcta agactgtggt
    33181 aaaattttcc gaaagcgctg tttgatccgc tttgattaca attccagtat taatcgtttc
    33241 gagattgcca ccttgatcaa tctcgccaaa ggccgccata aacatcaatc ctaaaagaat
    33301 tggaaaggcc aatgtccaaa acaacatgga tttatttcgc agcagtattt ttgtgcgata
    33361 tttataaaaa tgccaaaaca ttatttcccc tcctctagtc tctcaaatcc ttgccagtaa
    33421 tctctaaaaa gacatcattc aaggacgctt ctttggttgc tatttttccg taactgatct
    33481 tttcttcttg taagattgta agaatcggca acaatactgt atttagttca gtagttgtta
    33541 cttggagctg acctttttta taagcgattc catcagaagc tattgttcgc tgacaacacg
    33601 ttttctggga gtaatgggat gtcgatcgtc aaacgatccg ccgtccgtac catatctttt
    33661 agagattctt tggttccttt agcaatcacc tgtccttgat ccatgatcac gatctggtcg
    33721 caaagaattt ctacttcttc catgtaatgt gttgtataga caatggttgc tccttgtcga
    33781 ttcagctcct tgatactttc taaaatttta ttccgacttt gggggtctac agctactgtc
    33841 ggctcatcta aaaaaattaa ccgcggcttg tgggcaatcc cacatgcgat gttcaaccga
    33901 cgtttcaagc caccactaag ttctcctgga cgaaacgaaa catatttttc taatcccact
    33961 aattgaatca cctcttcaat caaggctttc cttttctctt tttcggaaac atataaaccg
    34021 caaaaataat cgatattttc ttgcacactt agttcgtcaa agaccgcaac gtcttgagga
    34081 acgaccccaa tcattgcctt gacagcataa ttttcaggag tcattgttgt cccgaacaat
    34141 ttaatggttc ctttgtcata atttaacaaa gaaagcatac agttgatggc agtcgatttc
    34201 ccactgccgt ttggtcctaa cagacctaag acttctcctt cttgtacttg cagatcaaaa
    34261 tgattcaatg ctacaaaatc tccatagcgt ttgactaatt tatcaatttc gataatcatc
    34321 atgaaaaact ccttccaaat ttcttgttga tctgactata ataaagtgca agaaaagaaa
    34381 ctagtgacga ttggctaaac cacacatgac atttgtcaca atgaaggagt ctgctatgca
    34441 taaatctcgg ctactcaacg aaactagctt tctactggtt atctcattgc ttttgctggt
    34501 aagtcaagga ttcgttgcta tgcaagttat ctatttattg atcgccgttt tattcactgg
    34561 tgtcagttat ttactgccta caaagccaaa ttggattttc caagttattc tatttctttt
    34621 atgtgccttt ttacctgatc ttgtcctgtt tctgccagcg acgatgcgag tgatcgcccc
    34681 tgaagaaagc caccttgatt acttgttttt gttaggtttt ttgttgattt cgacggttgg
    34741 taatcatccc cccatcaagg tcgcctttgt cgtaattttg ctgttttttt ccttttactt
    34801 acgaaaaaaa gatcaacgaa cgaccgatct tgagaaaaag tacattgtct ccaaagatac
    34861 tcattgggaa aaacaacaag agttatcgga acaaaataaa gcattggccc tgtcacaaga
    34921 aacacaatta gccttgcaaa ttgtggaaga acggaaccga attgcccgag acatccatga
    34981 caatgtcggt catctgctgt caagtgcgat tttacaaatt ggtgcgcttg aaatgatcaa
    35041 tcaagaacca aaactaaaag aaccattaga aaatttaagt gccaccgttc acactgggat
    35101 ggatcggatt cgtcaaagtg tccatgacct ccatgagaca tcgatctctt ttcaacactc
    35161 cttagagttt ctgatcgctg attttccttc ttctgttacg attgaaggaa acttgtttga
    35221 aggattgaat gaaacgcagc aaaactgctt cttaatggtc atcaaagaag ccttaaccaa
    35281 cagtatcaaa catagtcatg ccaaaaccgt tgttttacat tttcgaacgt tgccggcctt
    35341 ttatcgcttg caaatcacga atgatggcac cagcccacaa aaaaattctg ctcccggtat
    35401 tggtttgaaa acgatgcgtc aacgtgttca agaacaaaag ggacaagtcc atattttcca
    35461 agaaaaccag caatttcagt taacgatcat tttaccaagg gagggacacc aatgataaca
    35521 gtagtcatta ttgagatgat ccatttgtga cgaaatcgtt acaaaccatt ttagaaagca
    35581 cgaaagagat ccgtgtgtta ggcattggtc attgtgccaa ggatgcgcta gtcttatacg
    35641 aaacccatcg tcccgatgtt ttgctgacgg acatccgcat gcctaaacaa acgggcatcg
    35701 atgctgccaa agaaattttg gctaaatttc ccaaagccat ccttttgctt ttgacgacat
    35761 tcaaagatga agaatacatc cgcgaagcat tttccatcgg tgtcaaaggg tatttaatca
    35821 aacagaactt gcaagcgatt attccttctg tcaaatccgc ttataatgga caggccgtat
    35881 ttggtaatga aatcatcgag actttcactc aaatgatgaa gaataaaccg acgattgata
    35941 gtcatgctta tgcttctttt agtgaacgtg agcttgcaat catcaaagaa gtagctgccg
    36001 ggaaaaacaa taaagaaatt gcagatgcct tgtacttgag tgacggcact gttcgcaatt
    36061 acatcagcca attactagaa aagctagatt tacgggatcg cacgcaattg gctatctatt
    36121 tttatcagca tttgcaataa gtcttatcta acacaaaaaa acctgaatga tgctccttcg
    36181 catcattcag gtttttttca catcttctat tcaattgtcc cgcaaaccat ttcctcacga
    36241 ttgaaagaat caaggattcg cagggctgcc aggattcgtt ggattgttcg gcgtgttagg
    36301 cggaaaaata ggcgccttgt ctaacgtttt ttctagtttt gattcttcac gatcgtctcg
    36361 ttctacatca ggactttcat tcaatttgaa ttctttttcc atctcatcta actttttaaa
    36421 gtcattgcca gattcttttg ctaatttcgc taatttatct tcggtttctt tagccataaa
    36481 gtaacgcccc ccttttgatt tcagcttagc atatcacact gaaaaaacaa aagaaaacgc
    36541 taaccaacga tcatttttag ctatttctca tctttaaaat caagcatttt ttgccggtat
    36601 aaaatcgtag ggagaactgt tttccatacc aatcatcgga tctactaact ggttatcgat
    36661 cacttctgct gtaagaacga aatctttaga aacagcaggt tctacctctt ctttcttttc
    36721 ttctccaaca aatgaagaaa acagatctgg ttccactgtt tccattactt ggtttttcgt
    36781 ttcattcgtc ccactgtcat cttctacagg tagcttttct tttacaaccc gtggctcttt
    36841 tgtatagttc ccatcttcaa atggatcatt tccgaccaaa gaatcttcat aagctgctag
    36901 tttggtccgg agagtgatat tcattttatc attatccatg atccttgatt tcaacgtagt
    36961 catccggctt gctgattcat tcgctacaca agtgacatag gcttgctctt cccctaacaa
    37021 aatcaacagt tccttactgc gagtcactgc cgtgtagagc aaattacgct gaagcatccg
    37081 ctgatattga tggaccatgg gcaaaataac catcttgaat tcactgcctt gtgatttatg
    37141 gattgagcaa caataagaaa gagtgatttt attccattcg tttcttttat aagtgacctc
    37201 atttgcatca aactggataa ctaattcatc cactttatct tcagaatctt ttgccaagat
    37261 gatacccgtg atttctccca tatctccatt gaatacattc aattcgggag tattgactaa
    37321 ttggaggact ttgtctccta tccgataaac ggtgtcgttc caatgtactt ccttttttcc
    37381 aggtttccct ggattgaata tctcctgcat cattttattt aacgcatcga tccctgcagg
    37441 tccccgatac attggtgcta atacttggat atcttgtgca cggaatccct tatttttcgc
    37501 tttttccaca attttagaga tcaagggttc aatttgataa gcatcacttc ggaaatagga
    37561 acgatctttt tgattctttg tgaaatcttt tggcagacgt ccttctttga tctcatgggc
    37621 aagaggaata atactagaac catcaccttg ccgataaatc tgattgagtt ccattttggg
    37681 aatttgttcg atttccaaaa gatcatggag tacttgacca ggaccgacag agggaagctg
    37741 atctttgtct cctactaaga taacttgcat attcgtcgga atcgctttga ataatgtgtt
    37801 agccagccat gtatcgacca tagacatctc atcaacaatc aaaagtccgc cttctaattc
    37861 tttcgtactg actgaaggtg ttttttcccg tccattcagc cccagaagac gatggatcgt
    37921 actggcaggc agacctgtcg tttcgttcat tcgtttagct gctcggccag ttggtgctgc
    37981 aagtagaata ggaaaaattt cttgggtata ttttgaagga tccaattcca gtccgttcaa
    38041 ctctgcaaaa agattgacga ttccgttgat cacagtagtt ttccccgtac ctggtcctcc
    38101 tgttaaaata aatagagggg aacggattgc ttcagcgata gcttgttctt gcgaaggtcc
    38161 atactgtatg ccgaatattt tttcgatacg ccgaatattc ttctgcactt ctttttcagg
    38221 ataatggatt tcttttttac gactgagaag gcgctggatc gaattaccga tcccccattc
    38281 agaaaagtac aaactgtttt cgtaaagatt agtctcttct tgttgtatct ttccttcttc
    38341 aacaagttgg ataattacag tagcaacctg atcaggctgg atttcaattg ggcgacttgt
    38401 ttctaaaata cggatcgttt gctgaagcaa tgtctccgcc ggtacgtatg tatctcctgt
    38461 ttgaactgca tgctgaaaaa tctgatgcaa gatagctgct cgaattcttc tcggtgaatc
    38521 tgctgcaata ccgatttgtt cggctagatt atctgcacgt ttgaacccga ttccttctat
    38581 gtcttcgact agctggtacg gattttcttc gatgatatcc aaagactcgt tcttataggc
    38641 ctggtagata ctgaaagcta actgactacc gaacccgtaa cgattcagtc ccatgatcac
    38701 ttgatccatc ccgtggttta gccgaatcgt ctcagcaagc atttctcttt ttggcttcgt
    38761 caaaccggag atttgtttta ataccgaagg atcagcaatg attttttcga tagcatcttc
    38821 tcccagcaat tctacaattt tttctgctgt tttcttcccg attcctggga atttttcgct
    38881 ggaaagatac gctacgatcc catttgcagt tgttggcttt tcttgctcat atctttcgag
    38941 tttaagctgc cttccgtatt taggatgatc cactaattct ccataaaaac gataagtctc
    39001 attttcttga atatcgccga aacttccggt gactacaatc tctttttcac ggtaatcagc
    39061 tgtggtttca ctgatgtgta ccaaaagcac tttgtaaaaa ttactaggat tttgaaaaaa
    39121 aatcgcggca actgtaccaa cgaaataagg catttcttct ggctgcatcg gaagccgcct
    39181 ccttcttcta tagtaaaggt tctggtgctg tttcagttcc taaaaaatct gtttggttcc
    39241 atactttcag ttcatatggc aataatctgc aaggctcttc agtctctaaa atcgtcaaac
    39301 tactattgaa caatccaccc atttcacgca actcacttag ctcttttcct gtcatccatt
    39361 gaatggctgc agttaaagaa gctccatgtc ctacgaataa aagtgggcca tctttatgtt
    39421 gggcaaccgc atctgctaca acagtttcga tacgtgtgat tgccgattca atcgtttctc
    39481 cgtgaaaagg agaaggatca tacagatcta aatgattacg caaattcggc agattatccg
    39541 gatatttctg gtacatttca tcgatcgact gaccttctaa agtacccagc cctagttctc
    39601 ttagcttatc tgtataaaaa atctctaccg gacgagcaag ctctttgttg ataccttctg
    39661 ctgtatcacg tgctcgcttt gctgtactgg aataaattgc tttaaaagga acatatttta
    39721 cctttttccc aaatgctttg atttcttcgt aactttgtgg caaaagcgga gaatcgccat
    39781 tgctcccttg aaaacgtctt tcctgattcc attgtgtctt cccatgtcgg gtaaaatata
    39841 gttccatacg cagcaaactc ctgtctttct tttattttac tctataataa actgtctaat
    39901 ccagcttgtc attttcatag tgatgcgtca attctaaatt agggaatcct tgctggcgaa
    39961 gtgcttcata cactatcatt gcagctgtat tcgataaatt caatgagcgg acatgctcat
    40021 cattcatagg gatgcgtaaa cacttttctt cgttttcacg cataaatgtt tctggcaaac
    40081 cggttgtttc ttttccaaat aaaaagaagt gctcttctcc atcagcaaaa ttttcgtcac
    40141 tatatacttt atgggcaaat tttgagatca aatgtagttt acgattacct aaaaatgtta
    40201 aaaaagcttc taaattactg tggtaagtaa tatttacatc attccaataa tccaaccctg
    40261 cccgttttag atgtttgtca tcagttgaaa aacctagtgg ctcgattaaa tgaagcggag
    40321 tattcgtggc tgcacacgta cgggcaatat ttccagtgtt tgcagggatt aatggttcaa
    40381 ataaaacgat atgattcatc aggatcattc ctctcatatt tatgttcgtt tctattttct
    40441 gacaatttga tttttgcata aaaaaaaacg tatcaactcg gtgtcagtca ctgcgagtcg
    40501 ttacgttagt gaagctcttc tcactaactt ttatcagaac aggaacctga taaaaaccaa
    40561 tgaaaaaaca acttgttgct aatataacac cctcaacttt tttttgcaac ctctactcat
    40621 taaaaactgc tagaaaaacg ttttctctta gttcctttta ataattctat gtcaattgta
    40681 atcttcttca aaggattctt atataagtct gctttgattc gctcttctac ctgccattct
    40741 aaaggtgaca tggccaaaag atctttttga ttttcctctg gtatctcaaa ttcataggtt
    40801 acacgctgac gttcagttaa tttcattgct tcagcaaact tggctactac acgttcattg
    40861 gaataactct gtttttcagg ttgattcgga taaaaagcct gacgtagttc ctttaaataa
    40921 ttttcttgtg gcaccacctt caacacttct ccatcttctg agagtacacg ttggaattct
    40981 tggtaatgag aaggcgaaaa gatatttaaa accgcgttga atgtttgatc agcaaacggc
    41041 aaattggtca aatcagctac acaccaaaat gcttcggcag attgttctgt agctgcgtag
    41101 actccttctt ttgaaatatc aaatccaaca gcattttgaa tctttttcaa ttttgaaagt
    41161 tcattcaaga agctaccttc tccgcagcca acatctaata gcgagacttc ttcaggtaaa
    41221 tgctgcgcaa tagtctcaat aacgggctga tacatgccag attggatcat tctacgcctt
    41281 ggaacgaaca ttccttgatc gtattctgtt ttaaccgcat gggataaaaa ataaagtgtt
    41341 ccttttttgg aaaggtcaaa acggtgatta tttttgcata tcaagccatt atcagacttt
    41401 tttacttctt cttggcaaac cggacagcgg aacagctgat gattttctgc caaaaactgg
    41461 cgtcctcggt caattttttt taacatagtt ccccctcatt tctgttttat cttaccgtta
    41521 gatgctcact ttggcaactg ctaaaaatat gatacaatgc ctagaagaaa gtgaaagata
    41581 aaggagaaga ttagatgatc aaagaatttt gcgctgaaaa ctacacatct attcctctag
    41641 caattgctag aggagccgat cggatcgaat tgtgtgacaa cttagcggtc ggtggcacaa
    41701 ccccgagtac tggtgtgatc gaagaaacgt taagttatgc tggggaaaaa gatatccccg
    41761 taatgacgat tattcgtcct agagggggcg acttcgtcta taacgatatc gaattgaaaa
    41821 tcatgcatac tgatttgatt gaagcaaaga aattagggac ggatggcgta gtatttggtt
    41881 gtcttactcc ttcaggctgg ctagacgaag aggccttaga actattgatc gaaacagcag
    41941 aagggttgca gatcaccttc catatggctt ttgacagtat tcctgaagat cggcaattcg
    42001 aagcaatcga ttggctggta gaacatggtg ttgaccgtat cttgactcat ggcggtcctg
    42061 ctgacaaaac tattgtagaa aatttcccac ggcttaaaga attgattgcc tatacagatg
    42121 gccgcttaat cattttgcct ggtggtggtg tcactgcaga gaatgcagca catgtagcta
    42181 aagaattgaa cgtaagcgaa gtacacggaa ccaaaatcgt ggatcttcag ttataatctt
    42241 ataaacaaaa aaacatttgc aacaggcata atgcttattg caaatgtttt tgttttttta
    42301 cataaaatag cccataaata aagaagcaat attgaaataa atcagtacac tcgtcaaatc
    42361 gctcaaagtc gtgataaagg gaccgcttgc tactgctgga tcaaatccta gacgatccat
    42421 tagcatcggg attaaactac ctgcaagatt cgccactgtg attgcaaata gcatagccat
    42481 ccctatgaca aaccccaaga taaaattatg tttccaaaaa cctacgatta gaaaaatcgt
    42541 gatccccgta acagctcctg tgacaacgcc ggtcaagact tcagccaaaa caagtcgaac
    42601 aaatccatga tctttctcat cagaaatcgc aagtcgacga acagccacag ctagcgactg
    42661 ggttccagca ttaccggcag ttcctgtaat cagcgaaatg aagactgcta aaatactcgc
    42721 ttcacttacc aagtcttcat agtgactgat taaagaagcg gtcgacatac ctaaaaacaa
    42781 cagagtgatc agccaaggca aacgtcgaga cgctgccttt actggatttt cattaatttc
    42841 ttccacattg accccggcaa gtcctgaata atcgctagca gcttcatcat caataacgtc
    42901 gatgatatca tcgactgtga cgattcctaa caaatgatca tcataatcgg taacaggaag
    42961 agctaagaag tcataatctc taaatgtttg tgccacatct tcttggtcgt cgcctacatg
    43021 aactgaaagc actcgttcgc tcatcagatc agaaatcatt gcgtcgtcat cattcacgat
    43081 taggtcgcgc agagaaataa cccctactag ctgaccttct tgattaacga catagatgta
    43141 atagattgtt tcagctacat ctgcttcgtt cttcagcacg tacatcgcag agcgaacagt
    43201 ttgattggct acgatcgaaa caaattcagt tgtcatgatc gcgccagccg tttcatcctc
    43261 gtaatggaga agttccttga tttcactggc atcgtctgcc gacatcaaac ttaaatattt
    43321 agcaatttgc tttttatcca gcgtattaag caagtcaaca gcattatctg tatacatctc
    43381 agaaagcata tcagctgcat agctaggacg catttcatct aggtattctt tcatatgttc
    43441 gttgtcttct tcaatcacat cgaacatatc cgctaattct ttaggcgaca gataggaata
    43501 gatcagctgc ctgttctctt ctgtaagaga ttggtagaat tgcccctgct catagatatg
    43561 catctctaaa aaactgtttc tgaatgcctg aatattattt tcccttaaat cttgagtcaa
    43621 ctgggcaaat ctttcctcta attcttgatt ttcatccaaa ccaatctcac ctcgccttat
    43681 tcttcaaacc gtctcacaac tgcctccatg tctgcggcta aaggctgctt gaactccagc
    43741 aactcttctg taaatggatg gtaaaaacgc agatcataac aatgaagggc ttgtcttttt
    43801 attcccagat ccattcgccc gccatacata tcatcaccga gcagggcaca gccaattgcg
    43861 gaaaaatgaa ctcttatttg atgtgtcctt cctgtatgca gctggatatc aactaacgcg
    43921 atttctgtta gtcgcttctc tatccagtac tcagtctgtg ccgaacgtcc ttcatcagag
    43981 accattcgct tcaatagcga agaataatct cgggcgatcg gcaaatcgat atgtccatgt
    44041 gccgataaat ctgaaagcct tccactgact aaggcctgat attttttcac aaacttcttt
    44101 tcacgaagtt gcatatctaa ttttgcgtgc gcaaatccat gctttgcaaa aagcatcaaa
    44161 ccagatgtat cccgatctag tcgcgtcacc acatggatta cttggttttc gtaaccttgt
    44221 tttttataat aagcctttac acgattagcc attgtcccat ttggatggta ttgggcagga
    44281 atagacgaaa cgcctgctgg tttatttact accagaacgt gttcatcttc atacacgata
    44341 tcgatcggtg tttcatccaa aagtacggtt tcatgttctc cttcagcagg aatcacaatg
    44401 gttaccttat catccttagc tagagaaaac aagacatttt ctacttgatc attaactttg
    44461 atttgaccgc cttgaaattt tattttcgct aacagaccct ttgagatccc tttttctttt
    44521 aaaaaatatt ttacttgctg agaatgatct ttctcataat cccatgtaaa ctgcatatca
    44581 atcctcacta ataaaggcat ctttcacacg atgccaaaaa tgcatatgtc gataagaagc
    44641 aaaatggatc cgttcttctg ctattcgata ataaattgag cggatatttg cttttgcgac
    44701 attcaattga tcaaccgtta caagataatc ttctgtattt tccaatttta tttctagcca
    44761 ttcatcatgg gcaatcacga tcggagaacc caaagtacga aacactcgat tattcagtga
    44821 agcaatctct gccaactgaa aggcattgat actaggatgg atcacagcac cgccgacact
    44881 tttattatat gctgtcgaac cagtaggcgt agaaatcgtc aatccatctc ctctaaaact
    44941 ttcaaacagc tcgtttttga tgtatacatc ggctaccatc gtacgattgg cccgtttaat
    45001 cgttgattca ttcagtgcta aaaaatgctg gtccggtgtt tcatccaaat aactgatccg
    45061 aacatccagc aagggataac taacactttg ttcccggttc gttcgtaagc tgtctactag
    45121 ttcttccagc tcgtaatccc gccaatccgt gtaaaaccct aaatgacctg tatgcacgcc
    45181 caagaaacgc acatcattca gacaatgact gtagtgatgg aacgccgaca ataatgtgcc
    45241 gtctccgccg actgagatga ccaattctgg ctcatgatca tctcgttgaa tccctgcttg
    45301 ttctaaaagt gcgttcaaac gttctgtcac ttctatagtc tttgcttctt tattatgaac
    45361 gatcgctact ttcatcccct gcctcctctc tttcgctttc tttcttcaaa agcgttcgat
    45421 agtagacatc atctgatttt cctcttccat gggagaataa ccgctgtgct tcttggattt
    45481 cttcacggat actggacatc tcttcatcca actgataggc agcttctgcc gcacgctgaa
    45541 gtcgttcact catttcttct ggaaaaacgc cttgatattt ataattcagc gaatgctcaa
    45601 tcgttgccca gaaattcatc gcaagagtcc gaatctggat ttccgccaaa attttcttct
    45661 caccgctgat cagctgtaca ggatactcga tcaccagatg ataagaacgg tagccacttg
    45721 cttttttatt tgtgatgtaa tctcgttctt ggagaacttt catgtctttt cttttacgga
    45781 tgatctcaac aacttgatgg atatcttcta caaactgaca catgatcctt aacccggcaa
    45841 tatcagacat ttcttcttct aaacgtccca ttggaatatg ccgtaattgt gattttgtta
    45901 caatactatc gactggcttg actctcccag tgacaaattc aatcgggaca tgtcgatttt
    45961 gttcacggaa ctgtttgcgg atcccccgca gtttcacttt caattctgat acagtttgtt
    46021 catatggtgc taaaaatgct tcccaatctc gttccatagc tgatacctct acttcattaa
    46081 cattcattgt ttattctacc atatatgcaa agttttttcg tactcttgat ttatgcattg
    46141 accaaaaaaa gaaaattagg caaaatagac aaaaaggagt cgatcaaatg acacaaatgc
    46201 ttgaaatcga atataaatct atgctgacaa agcaagagta cgagatattc atcgctcact
    46261 atcaattaac agataaagat ttccgtgtac agactaatat ttatttcgat accgttgatg
    46321 aacagctgaa gaaacaaaat tgtggcctac ggatccgttt cgttggtcat caagctgaat
    46381 atacattaaa aacacctgct gaaaaagggc gtctcgaaac aacggatacc tttataacag
    46441 aagaagcaga agcatttatc ttcgaaaaac gtctgcctcg ttcaggcgca gtttttcaaa
    46501 aactttctga tttgaatatc gatcctgctt cattaatcca aaccggaaag cttacaacaa
    46561 aacgggctga attccctatt gaagaaggac ttttagctat tgatgaaagc tggggagaca
    46621 atctacacga ttttgaactg gaactagagg taggggatgc atctgtggga aaaattgctt
    46681 ttataaattt tttaaaacgt ttttctttac cttatcgtcc tgctaagaac aagatccaac
    46741 gtatgcttga agctaaaaat tagaaacctt tcattttcac aaggcacttg agttcatttt
    46801 tttacttttt tctgttaagt tataggatgt gaaagaatct agtcactagt tgtatagaat
    46861 ctattttggg ggagattcat atgatcgaaa tctatttatt tgttaatcct ttaggggaat
    46921 actgtttcga aatggaacaa cagttattac aatttatcga agaagagtac gggacaccat
    46981 caaaagaaaa gatgcaattt cgctttcttc ctttggtcaa tttgcagaca attggggatg
    47041 tcatgcaaca aaaaggaatt tctcagaaca atcttgagga acgtaatcac ttattctcaa
    47101 tgacttattc tgcagcattg gactgcaaag cggcgcagtt tcaaggaaag aaaaaaggcc
    47161 gccgtttttt gatcaaattg caagaagcag tcggctgtaa tgacatacca tattctcaag
    47221 aacttgctga atccatattt gaagaaatcg gcggcgattt gaatatgttt aaagaagatc
    47281 gtcagtctga cttcgttaaa gaagtattct tttctgatca aaaaattgct cgggaaatgg
    47341 gcatcacaaa acacccttct gcagtcgttt acaattatgc ctgcgaccgt gattttggtg
    47401 ttttagtaga agtaaaaggt acaagtagcc tttggagaat cacagagctg tgtaaaacaa
    47461 cagataactc atatcaagtt tttaatggtg aacatttaca tacaaatgat tctcagttcc
    47521 attcaagaac tagccattta cacttgcttt ctaattaaaa aaatacgtcg actcccttac
    47581 aagagagccg acgtattttt ttcgtttcta ttcgagaaaa tcaaaaatag aaagccttat
    47641 aaacgatcga tcaattcttc taattcattt agacgttgtt caaacatcga cattgcatct
    47701 tcaatatatg cagcctgtgt catatcaact cctgcttttt tcattacttc cacaggataa
    47761 tcgctgttac ctgcttttag gtaagccaag tagttctcta gtgcctctgg ttcctggttt
    47821 agtattttct tcgccagtgc tgaagcagca gaaaagccag tcgagtattg gaaaacatag
    47881 taattataat aaaaatgcgg tatccgtgac cattcgaatt taatttctgg gtcttcttcg
    47941 actgctggac cataatattt tgcattcaat ttaccataac tatcacttag gtattcacta
    48001 gtcaatggca cacctttttc atcttccgta tgcatgaaat gctcgaattc agcgaattgt
    48061 gtctggcgga aaactgttcc tttaaaccca tccaagtaat ggttgagtac ataagcccgt
    48121 acacgagggt ctttttctgt ttccaataaa tactccgtta ggatattttc attcgttgtc
    48181 gaagcaattt ctgctaaaaa gatggagtag tcgccgtaca cataaggctg gttcgaacga
    48241 gtgaaataac tatgaacact atgtcccatt tcgtggacaa gcgtaaatag ctgatccagc
    48301 gtatcatgcc aattcaataa aatatatgga tttgtgtcat agcttcccga agaataagca
    48361 ccgcttcgtt tccctttatt ttcgacaaca tcgatccaac gttcagagaa tgctttttct
    48421 acgatggcca tgtattcttc acccattggt ttcagtgctt ctaaagcttt ttcttttgct
    48481 tcttcgtacg taaaggtaat tggagcttcc cctaagaccg gtgtataaag atcatacatg
    48541 tgcagttttt ccacttctaa taaacgtttc cgtaattcca tgtatcgatg taacaaaggc
    48601 aaatgcttgt ttaccacgtc taccaaagta tcgtatacac tttcaggaat atgattattg
    48661 ctcaaagacg cttctctggc agagctgtaa ttacggactt tcgctttaaa attatgtcct
    48721 tttatatgtg tgcctaaagt agaagcaaat gtatttctaa attgttcgta aacactgtac
    48781 aatcccttaa atgctgcttc gcgcaccctt cgatccgtgc tttctagcaa ctgaccatac
    48841 acgccatgag ataattggac tatttcacca ttttctcctt caatcgttgg aaaaactaga
    48901 tctgcattat tcaaaacagc aaatgtatca cttgaagcat caaagatttc acctgctcca
    48961 gcaagaagag attcctgctc agcagataag acatgggctc gattatctac tatttgctgg
    49021 atataatggc gatagacttc caattttggt tcttctttga aatattgcca aatctggtca
    49081 tctgacaatt gcaatatttc tggttcaaac caggaaacgg cttcactaac tttcgaaaac
    49141 agactgcttg ctcttgcata aagcgcttgg taatctgtat ttccagtgtc ttgatcgttt
    49201 ttaaggtgcg catatacata aatgacttca gtttggcgat aaaccctcaa tacgaattca
    49261 atcgcattta aaaattgaga agcgccttga tcaagtgttc ctttgtgttt ttcagattgt
    49321 tttaactctt ctgataattc caaatatttc tcatcaaact cttggtcgct tgaaaagatc
    49381 ttggtcaagt cccaagttaa attttcaggc aattcttctc gttttggcaa ctgctttact
    49441 tccatgaaat ccactccttt tcttccctta gctctatcta tcaaaataga tcatagtcta
    49501 acatccacat gtcacagcat ctaacttttc tactcattca tgtgtctgct tccatcaatt
    49561 ttagcataac taaatcttgt tttacataga aaaccgattc ctagcctatt tttatactta
    49621 ctattttttc aaagaagaca agtgtatgca ttcgttaaaa aactgctgat agaccatctc
    49681 ttcatcaatc ataggaaaaa gccattccct tttatgttct tttatttggc aaagccatga
    49741 gttgaaatca ggaactttct ggttttccaa ggcttcttcg tacaacattc gataaagaaa
    49801 aacctgttct tgtaaataaa agaaaaaccg acttttttga tagatccacg atgatagatg
    49861 caaaagatgt ttatgacgca agtagcattg ttcttgtatg gatactgtat tcttttgctt
    49921 gtgaataagt tggcgtgtca gccatttctt cccatcttca ctcacttttt ctattttttt
    49981 catttggttc attttaattt cgttactagt gaatatttct ttaaggtccc tactagaaaa
    50041 agtccagcag ctactgcaaa aggaaatatg ccctgataca gtctctgtaa tatgatgata
    50101 caaataaagc ttttgtttct tgagatcagc tttccagcaa tgcactcctc tttttctatt
    50161 atagtagcag caactttttt ctatcaatga gaattgcgat tgtccaagaa aagaattacc
    50221 aagtatccac catacttggt aactgttatt caaataattt tgggttcttt caataaatct
    50281 ctggtgagac agtctagaac attggatttc tattgctaga ttcgccaaca aaatatccgg
    50341 cctttgccgc aacctcggca aataggcttc caaaaatact ggttctactg actgtcccag
    50401 ccaatccatg aagcattcct ttaactgtaa gtgctctttt gattctcttt cactaaagac
    50461 cgaacagtta tctgattttt gatgggcaaa atgagcacat tttttctttc cctttttcaa
    50521 aatcactctt tttcgacagc cacgacaata atatttcttt cctttttctg catgtttagc
    50581 aaaacagcac ttattatatt cattcattgc aagcagcatt tttctccctc tgaacttatt
    50641 tacgtctaag aatttattta cacgaaaaaa gagggcgtga aaaagctatt ctgcatcaag
    50701 taataagaac agctaaacaa aaatagttcc caatattttt cgtttggttg ggcttgttac
    50761 cgcagatgca agcttttgaa cacatttata cgaaaaaaag actgcgacac aatttttgtc
    50821 acagtctctt actataatgc tgatttatac aaaataaaat ttcgttcgct cgatagcatt
    50881 acgcgccatc agacattgtc catattcatt caatgtttct ggtgtacgat cgctcttttc
    50941 agcaaactcc aaaattcgag caatctgatt ctctacatcc gcttggttga gattttccat
    51001 ccagaagtga acgactaggt aatagcgctc ttcgaataaa tacaaatcag tccaagcaga
    51061 ctctagttcg acatctgttg caagttggat cacttgttca aaatcttcaa aagcaaaagt
    51121 tgcttgtctt tcctgttctt ctagggcgct tccagataat tcttcctccg gatcgctgcc
    51181 aataatctgc tgacgaagaa agtcactcac atcttctgaa tcaacagtag aatcttctga
    51241 ttgagaaatt tcttcatccg gaagattttt actgataaac agttctaatc cgtctccttt
    51301 tggcaaaact tggaaagtga cagcttcgct tcctcggaac tcctcttcga tatcaacttc
    51361 ttccaaaata ctataaaaga aattttcgat ttctttgtga ttgccaagta aatccaaaaa
    51421 tgaaacgccc cgttcctcta aatcttcatg accaattagt acacggatcg tattttcatt
    51481 aatatgttcc atttccattt tgctacacct cactttgcca caatgtttaa tatagattca
    51541 ttgtagcgaa atcagcttaa atgtaaagaa aaaaaatctc gctgttaagc gaatttaata
    51601 aaaccagcgg atttttcaac ctttcaagct atcttattta actataacat ttttggcaga
    51661 cgaatactat atagccgact tttcagctaa ttttccctct ctatttttcg tgcaatcatc
    51721 atcttctact acctttttat acatctaatc aagaatactt tttgtaaaaa caaaaaaact
    51781 tggaaaaact atctttgtaa tagt
//
LOCUS       DAFBQH010000010        49982 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.010, whole genome shotgun sequence.
ACCESSION   DAFBQH010000010 DAFBQH010000000
VERSION     DAFBQH010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.010"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(84..263)
                     /gene="rpmF"
                     /locus_tag="LK694_000686"
     CDS             complement(84..263)
                     /gene="rpmF"
                     /locus_tag="LK694_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBK5051577.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(321..890)
                     /locus_tag="LK694_000687"
     CDS             complement(321..890)
                     /locus_tag="LK694_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBK5051578.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1076..1978
                     /locus_tag="LK694_000688"
     CDS             1076..1978
                     /locus_tag="LK694_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK5051579.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2013..3797)
                     /gene="pyk"
                     /locus_tag="LK694_000689"
     CDS             complement(2013..3797)
                     /gene="pyk"
                     /locus_tag="LK694_000689"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBK5051580.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3871..4833)
                     /gene="pfkA"
                     /locus_tag="LK694_000690"
     CDS             complement(3871..4833)
                     /gene="pfkA"
                     /locus_tag="LK694_000690"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBK5051581.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5001..8315)
                     /gene="dnaE"
                     /locus_tag="LK694_000691"
     CDS             complement(5001..8315)
                     /gene="dnaE"
                     /locus_tag="LK694_000691"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBK5051582.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8546..8731
                     /locus_tag="LK694_000692"
     CDS             8546..8731
                     /locus_tag="LK694_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBK5051583.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8793..9989)
                     /locus_tag="LK694_000693"
     CDS             complement(8793..9989)
                     /locus_tag="LK694_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBK5051584.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10159..11595)
                     /locus_tag="LK694_000694"
     CDS             complement(10159..11595)
                     /locus_tag="LK694_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBK5051585.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVVTTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11888..12697
                     /locus_tag="LK694_000695"
     CDS             11888..12697
                     /locus_tag="LK694_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK5051586.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12883..15036)
                     /locus_tag="LK694_000696"
     CDS             complement(12883..15036)
                     /locus_tag="LK694_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBK5051587.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(15431..16279)
                     /locus_tag="LK694_000697"
     CDS             complement(15431..16279)
                     /locus_tag="LK694_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK5051588.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(16503..18443)
                     /gene="thrS"
                     /locus_tag="LK694_000698"
     CDS             complement(16503..18443)
                     /gene="thrS"
                     /locus_tag="LK694_000698"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBK5051589.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(18780..18959)
                     /locus_tag="LK694_000699"
     CDS             complement(18780..18959)
                     /locus_tag="LK694_000699"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBK5051590.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(19006..20829)
                     /gene="cadA"
                     /locus_tag="LK694_000700"
     CDS             complement(19006..20829)
                     /gene="cadA"
                     /locus_tag="LK694_000700"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBK5051591.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            21107..23386
                     /locus_tag="LK694_000701"
     CDS             21107..23386
                     /locus_tag="LK694_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK5051592.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(23479..24126)
                     /locus_tag="LK694_000702"
     CDS             complement(23479..24126)
                     /locus_tag="LK694_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051593.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(24305..25024)
                     /locus_tag="LK694_000703"
     CDS             complement(24305..25024)
                     /locus_tag="LK694_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5051594.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(25155..26306)
                     /locus_tag="LK694_000704"
     CDS             complement(25155..26306)
                     /locus_tag="LK694_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBK5051595.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(26523..27263)
                     /locus_tag="LK694_000705"
     CDS             complement(26523..27263)
                     /locus_tag="LK694_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK5051596.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(27267..27590)
                     /gene="rsfS"
                     /locus_tag="LK694_000706"
     CDS             complement(27267..27590)
                     /gene="rsfS"
                     /locus_tag="LK694_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBK5051597.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(27647..28240)
                     /gene="yqeK"
                     /locus_tag="LK694_000707"
     CDS             complement(27647..28240)
                     /gene="yqeK"
                     /locus_tag="LK694_000707"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBK5051598.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(28224..28874)
                     /locus_tag="LK694_000708"
     CDS             complement(28224..28874)
                     /locus_tag="LK694_000708"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBK5051599.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(28884..29195)
                     /gene="yhbY"
                     /locus_tag="LK694_000709"
     CDS             complement(28884..29195)
                     /gene="yhbY"
                     /locus_tag="LK694_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBK5051600.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(29211..30320)
                     /gene="yqeH"
                     /locus_tag="LK694_000710"
     CDS             complement(29211..30320)
                     /gene="yqeH"
                     /locus_tag="LK694_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBK5051601.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(30317..30844)
                     /locus_tag="LK694_000711"
     CDS             complement(30317..30844)
                     /locus_tag="LK694_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBK5051602.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(31024..31809)
                     /locus_tag="LK694_000712"
     CDS             complement(31024..31809)
                     /locus_tag="LK694_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBK5051603.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(31802..32671)
                     /locus_tag="LK694_000713"
     CDS             complement(31802..32671)
                     /locus_tag="LK694_000713"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBK5051604.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(32672..34051)
                     /locus_tag="LK694_000714"
     CDS             complement(32672..34051)
                     /locus_tag="LK694_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBK5051605.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(34059..34478)
                     /gene="fabZ"
                     /locus_tag="LK694_000715"
     CDS             complement(34059..34478)
                     /gene="fabZ"
                     /locus_tag="LK694_000715"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBK5051606.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(34503..34979)
                     /gene="accB"
                     /locus_tag="LK694_000716"
     CDS             complement(34503..34979)
                     /gene="accB"
                     /locus_tag="LK694_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBK5051607.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(34982..36217)
                     /gene="fabF"
                     /locus_tag="LK694_000717"
     CDS             complement(34982..36217)
                     /gene="fabF"
                     /locus_tag="LK694_000717"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBK5051608.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(36236..36973)
                     /gene="fabG"
                     /locus_tag="LK694_000718"
     CDS             complement(36236..36973)
                     /gene="fabG"
                     /locus_tag="LK694_000718"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBK5051609.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(36985..37902)
                     /gene="fabD"
                     /locus_tag="LK694_000719"
     CDS             complement(36985..37902)
                     /gene="fabD"
                     /locus_tag="LK694_000719"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBK5051610.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(38009..38233)
                     /locus_tag="LK694_000720"
     CDS             complement(38009..38233)
                     /locus_tag="LK694_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBK5051611.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(38286..39248)
                     /locus_tag="LK694_000721"
     CDS             complement(38286..39248)
                     /locus_tag="LK694_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBK5051612.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(39258..39698)
                     /locus_tag="LK694_000722"
     CDS             complement(39258..39698)
                     /locus_tag="LK694_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK5051613.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            40004..40969
                     /locus_tag="LK694_000723"
     CDS             40004..40969
                     /locus_tag="LK694_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBK5051614.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(41026..41256)
                     /locus_tag="LK694_000724"
     CDS             complement(41026..41256)
                     /locus_tag="LK694_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBK5051615.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(41342..42229)
                     /locus_tag="LK694_000725"
     CDS             complement(41342..42229)
                     /locus_tag="LK694_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBK5051616.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(42318..43541)
                     /locus_tag="LK694_000726"
     CDS             complement(42318..43541)
                     /locus_tag="LK694_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK5051617.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(43558..44604)
                     /locus_tag="LK694_000727"
     CDS             complement(43558..44604)
                     /locus_tag="LK694_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK5051618.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(44768..46495)
                     /gene="ptsP"
                     /locus_tag="LK694_000728"
     CDS             complement(44768..46495)
                     /gene="ptsP"
                     /locus_tag="LK694_000728"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBK5051619.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(46495..46761)
                     /locus_tag="LK694_000729"
     CDS             complement(46495..46761)
                     /locus_tag="LK694_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBK5051620.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            47240..49474
                     /locus_tag="LK694_000730"
     CDS             47240..49474
                     /locus_tag="LK694_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK5051621.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            49622..49942
                     /locus_tag="LK694_000731"
     CDS             49622..49942
                     /locus_tag="LK694_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBK5051622.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT        14284 a        10732 c         8730 g        16236 t
ORIGIN      
        1 ttttttattt accgactaca aaaaaataaa cctcaaggct ccatggttag ccttgaggtt
       61 ttacattttc taattaaaat gtattaagct tcttttgaca ttacgtcctt accatcatag
      121 taaccacaat ttgcacatac gtgatggctt tttttcattt caccacagtt tggacattcg
      181 tttaaccctt tgattgtcaa tttgtagtgt gtacgacgtt ttgctttttt agcttttgat
      241 gttcttctag ctggtactgc cattttgcta cacctccttg taaagtcttg tatctaaatt
      301 agacacaata tattccaatc ttacttgtta tcgtcgtctt ttgatttaga atcaaaaagt
      361 tcagatagtt ttgcaagacg aggatcaatt ttattctcgg cttcttccat ttttctgcgc
      421 tggtattctt cttcggaaac aacttcccaa tcattgcctt ttggaagttc tgttgctgct
      481 tcctcttctt ttgtcaatac ccgcattgga atcgccaata agatgttgtc ttcaatcgac
      541 tctgctaaat tcaataatgg accttcaatt agcaaaatct catctgcaga catttgttca
      601 tctctacttt ggaattgttc ttctgtcatg aatacttcgt ctacagaaaa atccatgggc
      661 agttcaacag gttctagtga acgtgttgaa ggaactgtca aagtcgtctg cacagtgtaa
      721 tgaacgatat agcctttctt atcaactgag accaaacctt cgacttccac tggcgcaaca
      781 tcaagtatta aattgtctcg cttcatgaga gcggctttca agtcgagcgt ctcatgaaaa
      841 gttaacggtg tctcctggta ttttctcaat tccaataaag accatttcat ttttcatcac
      901 tcctaagcaa caaaagcaat tatacagggc aaaaagcatg ttgtcaatga aattttcttg
      961 acaccttgca gttcataaaa aaacagaaaa atctttatta gaaagcctct gtttgctatc
     1021 atcctcagaa tcatttacaa taatcattat tccattaaag gagcggatga tcttcatgaa
     1081 cacgaaagat atcgaatact atgtccaaat cgtcaaacaa aaaaatttta cgaaagtggc
     1141 taattatttc cgtgtgagcc aacctactat tacatatgca ttacaacgtc ttgaaaaaga
     1201 gtttgacacc gtacttatta caagagatcg atcgcatcac gaattaatcg tcacgccttc
     1261 tggaaagcag ctgtttctcc atgcacaaac cattctccaa gagatttcta ctgcaagagc
     1321 tgaaatcact cagttgcaaa agaaaaagct tcgttttggc atgcctccta ttatcggcaa
     1381 tgaatacttt cctaaactgt caagttactt tatcaagcat gaattgatga atcaaattga
     1441 gattaccgat ggaggctctc gggacttata cgggatgatc cgacaaggga ggatcgattt
     1501 agctgtatta ggctcaacac agccaatcgt ggatcaatat ttagaaacag agttactttt
     1561 ggacaaacgt ttcatgatcg tcgtgccacc tacacatcct ctttccaagc aggatactgt
     1621 ctctttcaaa gatatagcag atgaacagtt tgtactattg aacgaacatt atgtccaccc
     1681 cgcagcattc actaagcttt ctaaacaaac acatactcat ccaaaagttg tttatcaaag
     1741 caatgacttg acgattttaa agagtatggt ccgagaaaat gtgggtatcg gttttttggc
     1801 agagattgct gtccatcctg aagatcattt agtttgcctc cctctttcag atgaagttca
     1861 gcctcgcttc ttgatttcat tggcaaaacg aacacagcag atcgtcacgc ctcttcaaga
     1921 acaagtagtc caagttatcc atgaattcgt tacagcacaa aaaaacagct cttcgtaaaa
     1981 gaagagctgt ttttattttt tattaagcta aattagatag ctgttgtttc gccacggtaa
     2041 acgatgccgc gacgtgaatc aacggtaatc aattcacctt ctgtaattgt tgttgttgcg
     2101 cctttagcac caacgattac aggaatatct tgtgcaattc ctacaactgc agcgtgagaa
     2161 gtcaatccgc cttcttcaac gataacagca gctgcttttt cgattgcagg catatattct
     2221 ttgtcagttg atggtacaac tagaaccatt ccgtcttttg ctttttttac agcttcttca
     2281 gcagatgaag caataactgc atttgcaacg acagattttt gtccaacgcc ttgagcttct
     2341 aataatttag aaccaatcat ttgaattttc atcacgttag ttgttccgcg ttcaccaact
     2401 ggtacaccag cagtgatcaa gattaaatcg ccttctgatg cgaaacctaa ttcaacagct
     2461 tttttagctg ctagttcaaa catttcatca gttgttgatg gtttttcagc aactgttggg
     2521 taaacgcccc agtttaacat caaaccacgt tttgtgcgtt cgtcaaaagt aacagccaag
     2581 atatctgcat ctggacgata ttttgaaatc atttttgcag tgtatcctga ttcagttgca
     2641 gcaacgattg ttttcacgcc tagatttttt gcagcacgag ctacagataa accgattgtt
     2701 tcagttacat ctgttttatc gaattcgttc aattggaatg tacctaattc tgataatgca
     2761 ttttcagctt cttggtcgat acgtgccatt gttgaaacag cttcaactgg gtagtccccg
     2821 tttgcagatt caccagatag cattgttgca tctgtgccat caaatacagc gttagcaacg
     2881 tcagaagctt ccgcacgtgt tggacgtggg ttttcttgca ttgattctaa catttgtgta
     2941 gcagtgataa ctgggatacc agcagcatta cattttttga taatgcgttt ttgaaccatt
     3001 ggtactagtt cagctgggat ttcaacaccc atatctccgc gggcaatcat aacaccgtca
     3061 gaaactttga tgatttcgtc aatgttgtcg ataccttctt gagattcgat tttagggaag
     3121 atttgaacat gagtcatgtc tttttcttct aagatttcgc ggatatctag aacgtcttgt
     3181 gctttacgta caaaacttgc agcgataaag tcgatatcgt tgtctaaacc aaaacgaata
     3241 tcgtcagcat cttttggagt aatacctggc aaactaatag aaacgcctgg tgcatttacc
     3301 cctttacgtg atcctagcat acctgcattt ttaacttcta cgactaattc acggtttgct
     3361 tcgtcttttt caatgatttg catatcgatc aaaccatcat caaataaaat gtgtccgcca
     3421 acgtgaacat cgtcaaataa tcctgggtaa gtaaccgcga ttttttcttt tgaacctaca
     3481 tgttcaggat ccatagagat gcgtgtttga tctcctactt caaatttgat gtatcctgca
     3541 cgaccaaaat cagcttctgt tgtgccttga acagttgtac ggatttcagc tccttttgta
     3601 tctaaaagaa taccgacatc ttttcctgtc tttttaactg cttcacggac catagtcata
     3661 cgtgataatt gttcttcatg gtcaccatga gaaaaattaa agcggaagac atttgcccca
     3721 gattcaatca attgagaaat aatttctact gagttacttg ctggtcctaa tgtactaacg
     3781 attttggttt ttttcattta aaaaactctc ccattcaatt ttgtttattt aaagctgtac
     3841 acagcttaaa tagcttggtt tctttcactc ttaaaatgaa agtgaattat ttagatcata
     3901 tagtgataag tctggtttat gtttgttgtt ttcaagtgta tcaatgatgt cagacgcaac
     3961 gatttcgttg tcatggatac cgacacataa tccacctttt ccttcttgaa gcaattcgac
     4021 agcgtaagca ccgaatttac ttgccaacac gcggtcgcgt gcgcttggtg ctccaccacg
     4081 gacaacgtgt cccaagatag atacacgtgt atgatagtca ccaaattctg aaagtttctc
     4141 agcgaattca tttccgccca ttacaccttc agccaagatg atcaagcaat gtttcttccc
     4201 gcgatcgcgg ccttcttgga tttttttagc aactgaagcc atatcaaaat catgttctgg
     4261 gatgatgatt tcatccgctc cgccagccac acctgaccat aaagcgatgt ctcctgcatt
     4321 gcgtcccatt acttcgataa cgaatgtacg aacatgagaa gtcgctgtgt cacgaatacg
     4381 gtcaattgat tctagtactg tattgattgc tgtatcaaaa ccgattgtga aatcagtccc
     4441 aggaatatca ttatcgattg ttccaggaat acctactgct gggaaaccgc gttttgtcaa
     4501 agccattgcg ccatgataag agccgtctcc tccgataacc actagacctt cgattccgaa
     4561 ttttttcaat tgttcgattc cttttaattg accttcttca gttgcaaatt ctggataacg
     4621 tgcagaatac aagaatgtac caccacgttg gatcttgtca cctacatctg ccacatcaag
     4681 acgacgaata tctccagcaa ctaaaccggc aaaaccataa ttgatcccgt atacttccag
     4741 tccttcgtaa atacctttac gaacaacggc acgaacagct gcattcattc caggtgcgtc
     4801 tccgccactg gtcaaaattc cgatgcgttt cataataaac attcacctca ttaaaatact
     4861 ttatcctaag tatgacttag gtttacaacc atttttgcgt taaccacgac taacattcta
     4921 acattatttt tctcacttgt cttgatttac ggataaaaaa atgaaaaaaa atgaaagcaa
     4981 gtagtatttt ttaccgtaag ttatttgaaa accacattat cttcgccaag cagataaata
     5041 agttgcccct ttatactctt gctgtcatcc acccaataag catctgacag aactactttt
     5101 cgttgcgttt tttcgtgata cataactact ggtatatatc ctgggtgttt ctgaaaaatc
     5161 acttgtaatt gtttcattat ctcgggatct tcccgttctt ctgtaatacg caaatagcaa
     5221 gtttgtgaag gatactgggc ttctaactgg ttaggatcag caaagctttc tccaattagt
     5281 tgcagttcct gattatattt gctgatttcg ctccgccctt gaacaaagat cactcgatca
     5341 acggcaaaca aattacgata tttacgataa agagttggaa aaactgtgac agaaatttct
     5401 ccactactgt cggttccttc aacaaaggcc atcagttctc ctttttttgt ccgaatttct
     5461 ctaacttcct tgatcatcac taataaattg actggttgat tagggacgat atctgtaatc
     5521 agctgaactt gtttagctaa acgaagacga tcaaaccctt ctgcaggatg acctgacaaa
     5581 taaataccta agtactcctc ttccagcttc aagcgttctt ctagtgtata gtcagcgact
     5641 tcttcttctt ttaatgccat gattcccagc aagtcaagac ttccaccact gtaaacgata
     5701 ttttggattt ttccttcgag atcaagcatc aattgtttcc tgttaggaga taattcatca
     5761 aaaactccta ctgctgtcag tgattgcagt aactctaatt tcagccaacg cttatcgatc
     5821 cgtatgagga attgatccac tgactggaac gggccatttt gttttctttc gtttaatata
     5881 tcttctacaa aatcacgccg aatacctttt atcgctgtca gtccgaatct gatttcttcc
     5941 ttattgacca actcaaaact ggaattactt tgattaatgg acggcgagga aagtttgagt
     6001 ttatttttac gtgcttctgc aatatattct ttcaattttg atgtgttatg ccgaacagaa
     6061 ttcatcaggc tggtaaagaa agcacctgga tgatgggctt ttagatacgc catttggaat
     6121 cccacgaatg aataagcaaa tgcatgtgac cgattgaatc catagtttgc aaatttttcg
     6181 atatattcat atacggtcgc tgctgttttt tccgaatatc cctgttgtaa tgctcctgat
     6241 acaaaatgcg acttttcttc atctaatacg tcttttttct ttttactcac tgcccgtcgc
     6301 agaatatctg actgccctaa tgtaaatcca gccatagtcg aggcgatctg catgatttgt
     6361 tcctgataga cgatcactcc atatgtgttt ttcaaaatcg ggatcagtga atcatctggg
     6421 tagcgaatgg cttcttttcc tttcttcctg gcaatgaagg tatctatatt ttgcataggg
     6481 cctggtcgat acaatgcatt gactgcggca atatcttcga tgttttctgg atctaatctt
     6541 cgtaaaacgt tccggatacc agcagattca aattgaaata ctcctgaggt ttcgcctcgt
     6601 tgaaacaaga gcagcgtttt tgcatcattt agcgggatct cttttaatcg gatctcatac
     6661 ccttctactc gcttaacagc tttcaaagta aaatcaataa tcgataagtt gcgtagtcct
     6721 aaaaagtcca ttttcaaaag accgattgct tctacctcat tcatcgtata ctgggtcaga
     6781 aaagcttcat cagaaccgcg ttggagcgga actagttcaa gtagattctt atcgctgata
     6841 actacacctg ctgcgtgagt cgaaacatgt cttggcaatc cttctaaaat cactgcagtt
     6901 ttgaataaaa gttggttttt ttcgcttgcc tgaactaatt ctactaattg atgcgaagtt
     6961 ttataagctt gggtcaatgt gattttcaat tgattgggta ttgcttttga ccagcggttt
     7021 gcttcacttt gcgataaacc aaacactcta gctgcatctc taagaaccat ttttgctgcc
     7081 attgtgccaa atgttgcgat ttgtgccata tgatattgtc catacttttc tcgaacataa
     7141 ttcaatactt cttcacgtcg attgtcagga atatccaaat cgatatctgg catcgtaaag
     7201 cgttcggggt ttaaaaagcg ttcgaacagc aaatcatatt ctaatggatc cacatctgtg
     7261 atttctaaca cataggctac taatgaaccc gcagcagaac cacgacctgc accggtaacg
     7321 attttgtttc tatgagcaaa agccattaca tcccatacga tcaagaaata atcatcgaat
     7381 cccatttgat ggatgacaga taactcgtag tccaaacgtt cttggtattc ctttgtatag
     7441 tcagtgattc ggcgttccac ttgcgaatag cataactctc ttaaatattc tccagcttgt
     7501 tttccattag gaactggata atgcggaagt aatttctgat ggaactggaa ttcaaatgaa
     7561 atcgattcag ctaaacgctg ggcgcctatt acagcttggg aaagacccgc tttttcgaat
     7621 cgtttgatca tttcttgttc tgaaagaaga tagacatttg ctgttagtgg taaagatgca
     7681 gataaatttt ctaaaggtat ttggcttccc tctttgatat gtttcatcac ttcaagtgca
     7741 aaaagttctt cttgttgtaa actagcgatg ggatgtaatg cacaaagcga ataattttct
     7801 tttatcacat actgatacca ttcaggcaat tgatcaggcg tgttttctgt caagccgata
     7861 taaaaatttc tagtaaaaac attttcaagt aaaaactgcc aatgaccgaa aaagttatcc
     7921 tctttattga aagcctcaga aacagaatga tttgccggaa caacaaacgc tagatggtcg
     7981 gtttctgtca tttctgcaag ttccaatttt tcttggatca tttttcggct ggaaagtttc
     8041 atcagctgct gataacctat ttcatctttt gcgtacgcaa gcaagccata atttttttct
     8101 tcataagtat aactaagatg taatccaatg attggctgta ctccttcttc tacacaaata
     8161 cgatagaatt caggtgcacc gtataagaca tctttatccg ttatggctaa tgaagtgtag
     8221 ccttgttttt ttgcttcttg tacatattct cgaatacgta tcgtactttg taaaagagaa
     8281 taagaagtaa ttgtataaag ttgtgggaaa aacatcttgc attccgcctt ttttttcgtt
     8341 aaaattcact agcgttaaaa ggtttgatcc ttttactttc attaagatag caggcgctga
     8401 agttgattat tccacttcat ttgcttgctt gtaatctgta ggaatgagtt ctttcgcaag
     8461 aactcaaatt ttattgagat ttggtctcgt ttctttacat tcaaagaaaa tctggtaaaa
     8521 ttaagataga aaagaggtgt tctttatgcg atatatcgtt acattattct gggctgtcgt
     8581 tctaggtcaa gtagtcggtt atattggtgc tgcattaaca agcggaacat atgattttac
     8641 tttgacaaca atcatttcat tcattgcggg tgtcattatc cttttgattg gtgcagttgc
     8701 gccaagaaaa gaaacctctg cccattcgta aaaaaacagc caagagcttg agatttctca
     8761 agctcttttt tattttctac tcctcaccac cattataatc tgctgcgaag aactttacga
     8821 aagttgaata aagaaagaag aatatttgac agaacaaaca aacttgtcac aataaaaaca
     8881 gcactgtaat tataagcatg cgcgactgtc gatcctagca tgggacctaa aacttgtcca
     8941 aagttgctaa acatctgatt gtagctatag attcggctga ctccttcttg aggcgtaatc
     9001 ttactgatca gtgtattgac agaaggcatc aaagcgccag ttgaaaagcc taaaataaag
     9061 cgaagtatac ctaattggag cggtgtagtg acgaatgcca taggtaaata acagcaaaat
     9121 gataaaatga gtcctccaat tagaactttt tgattcccaa tcttatcccc tagttttcct
     9181 agtttcggtg aagaaaagac agcggaaaca ccagctatcg aaacaatcaa gccgctgaca
     9241 aaaaggatat tttctgtact tccgctcaat tcacgaatat ataaggtcaa aatagggctg
     9301 atcgtagtga ttcctatctg taaaatcaag gtcgtaatga agagaccgat caaaatagag
     9361 agatgatcca tttttgaaaa aatttctttg gtgcttatca gatctttttt ctcgattgga
     9421 tggaaatctt ctttgactaa gaaaatggtc aataccgttg tgatcatcaa caaagcccct
     9481 gtgatcaaga atacattttc cataccaaac cactgagcca gtgcaccgcc cattgaaggt
     9541 ccgatcagat ttccagcaat cgctccggta gacaatgttc ctaacgccca tccgctcttt
     9601 tctctaggtg cctgtgaagc aatcatcgct gtggcatttg gaatatatcc agacaaaatc
     9661 ccattccaaa accgcatgat caaaagccaa tacgcgtttg gtacaaaagc caaagatccc
     9721 attgttaatg tcatccctgc tgctgcacgg atcatcatga gttttcggcc ttttcgatca
     9781 gctagatttc cccagattgg tgcaaccacg gcagatgcaa aagcagtcac agagattgct
     9841 agtcccgaaa acaattcgat ttgatctttt ggtgttccta gttgctcaac ataaactggt
     9901 atgaatggca taactaaact aatacttgca ccagtgaaaa aacagccaat ccacgcaatc
     9961 actaaatttt ttctccaatt aatctccatc tatcatccct ctttcgtctc ttttatcata
    10021 cctattttcg aataaacaag caacgatctc gccagtaaaa agagaaaaat cccgcaaagg
    10081 tcattttata tgtaacgttc tttttatgta aaacaaaaag agatcagaac aaatactgtt
    10141 ccaatccctt ctctttagtt aaattgttgc tagaatgatg aagttgataa tgaataaaat
    10201 atcaacgatc cacaaagcaa cagaaacttg attgtatttt cctttgatta ctttcacaat
    10261 tgtatagaaa ataaatcctg ctgcaatccc ataggaaatg ctatagcaca atcccataaa
    10321 gatcgatgca aagaaggccg gtaaagcttc ttccatatct agccatttga tgtctgcaaa
    10381 agaagccatc atcattactc caactaaaat caatgctggc gcagttgctt gatttggtac
    10441 aatggcgatc agtggagaga ataaactact taatgcgaat agcactgcca ctactactga
    10501 agtcaaacca gtacgtccac cggccccgat cccagctgca gattcgacat aagttgttgt
    10561 attcgatgta ccaaagatag cccccacaga agtagcgatc gcatcggcaa atagtgcttt
    10621 atccattttt gttttgaacc cacgaccatc ttccaacgcg atctcgtctt cttttgagaa
    10681 aatacctgtg cggcgtcctg taccgataaa tgttccgatc gtatcgaaag tatcagataa
    10741 actgaatgcg atgatcgtca tcaatacttg aggaatcttg gaagaatcac tgaataaaga
    10801 ttgcatgcct tcagaaccaa atgctgcacc gaaagttgtt cctaattctt taaatgaatt
    10861 acctaacgaa ttcgtttgcc aatcaatgga acttaaatca acaacaccca taaaaatacc
    10921 aaggactgtg gtcactacga taccgataag aatagcccca cggacatttt ttacaactaa
    10981 aatggctgtc agtaccaaac caatcacagc caataagata ggagcgttgt caaagttggc
    11041 taacgcaggg acgattccac catttatcgt aacatttgtc gcttttccgc cttcaacgac
    11101 agagctcgaa atagctgact ggtctgctga aaaagataag aatcctgcat ttttgattcc
    11161 gacatacgca acgaaaatac cgatcccgcc accaatcgca tgttgaagac tttcgggaat
    11221 cgcccgaata atcatttttc gaatatttgt gacagtaata aaaatattga ttaatccgca
    11281 aataaacacc attgccaagg cttgttgcca agaataacca agcccaaaaa cgaccgtaaa
    11341 agtgaaaaat gcgttcaatc ccataccagg tgcctgagca tatggtacat ttgcgaacaa
    11401 tcccattacc agcgtaccaa taatcgatgc aatgattgtc gctagaaata ctgcttggaa
    11461 gggcatacct gatgctgaca aaatcgtcgg atttacgaat aagatataac tcatggcaaa
    11521 gaatgtcgtt actcccgcca ttatttcagt tgatacattt gtattgtttt tctttaaacc
    11581 gaaaaacttt tccataaaaa gttccactcc ctgaattttt ttctgatgga aaactataat
    11641 aataaaaaat aagtaaaata gggcaaatcc atcgaacaat ttcaaattat atagacgttt
    11701 cattcgcttt tcaacgtttt tttaacaaaa aaagcgaaaa taatacttaa cgttcgtgtt
    11761 tagcttatgt ttagctattt atatagccat aactgtagtt tttgttagga atagctgatt
    11821 gctcttgtta attacctaaa atatgctaga ctaaaacaaa tgctagttga ggaaggaagg
    11881 acccttattg aataaaaaat taattgccat cgatctggac ggaactaccc tcaatcaaga
    11941 ttcgatgatt acatctaaaa caaaagagac tctaaaaaaa gcaatcaatg ccggtcatca
    12001 tgtcagtatt gctacaggac gcccattccg aatgagccat cagttttacc aacaattaga
    12061 attaacaaca ccgatggtta attttaacgg cgcattagta cacatcccta atcagcactg
    12121 ggatggtgaa agagaaacgt tgatcaatcg tgaaatcgct tttgaaatct tatcgcaaaa
    12181 aaagcagcta aatttagatt ttatcgctgc tgaaaatcgt gatacttttt ttattgacag
    12241 ttttgatttc tttgatgaaa aaatttttgc ttcttctcgt ccaggagaga agaatttgct
    12301 ttctcctaaa aatttgacga caaatcctac atctttgctt gtccgaacag acaaacgttt
    12361 tgcaggagct gtatctgctg aattaacacg tcaatttggc agttatgtcg atgttcggac
    12421 atggggtgga cctactgcca tcttagagat tgtttcaaaa ggaatccaaa aagcaaaagg
    12481 cgtgcaagag attgctaact acctgtctat cgatcaaaaa gacgtcattg cctttggtga
    12541 cgagcataat gatttggaat tgttggatta tgcaggttgg ggagtagcta tggctaacgg
    12601 tactgaccaa ctcaaaggca ttgcaaatga tgtcactcct ttgagcaatc aggaagacgg
    12661 tttagctgtt tatctggaaa aactacttaa actataatag agaattagat tccacaaaaa
    12721 agaggttgtg acaatttttg tcacaacctt ttttgccgag taaacggtgt tcaaaagcca
    12781 gtttctcggt atgaagtcaa atttaagcaa aaatgtgaga agctattttc acaaaatttt
    12841 tcttcatact cgaaactaag cggctttttc acgacctctt ttttattttg ccatgaacat
    12901 atcataatac gcgtccatta tttctttagc aatcgtcaag tttatgtggt cttgttcgtc
    12961 taataagttc ggcaagacaa cactgatggc aatttccgga ttgtctgttg gtccgtatgc
    13021 cacgatatta ctattgactg tcgcaatatc tggatgacct tccgcaaccg tttctgcagt
    13081 ccctgttttt gcggaaaggt ccattttcgc actggctaat ggtttggctg ttgtgtaagg
    13141 atcggtacca tggacaactt ggtggaatcc ttcacggagg atcgccatat tttctgcaga
    13201 aatattgacc gtattttcta ctgttgtgcc aatttctttc ttcacttcac ccaagtttcc
    13261 attttcgtca ttgccataga tcgctttgac taggtgcggc tgtactcgtt tcccgccatt
    13321 agcgactgtt gaagcatatt gagctaattg gattggtgta tatgtgtcaa actgtccaaa
    13381 tgccaaatcg gtaaagttac ctggagcata ccattctttg ccattatccg cattgaactt
    13441 cttcatataa tcaacagaac gtgagatccc aggagattca ttcggcaaat cgatcccagt
    13501 cgttgtacca agaccaaact ctttgaaaga gtctctcaat tgctgataag ctttagcttc
    13561 ttcatctaaa ctaggtagtc ccattccagg tgtatagtct agtcctaaca tctttaacgc
    13621 cactttgatc atatacgtat tagaagacaa ttccgatgct ttgactgcat caagagcaac
    13681 ttgtccgctt cgattaaaga cagagctttt ttctgaagca ccttgcagac gaatcggctc
    13741 atcaatcaaa acttgatttc cgctgatcgc gttacttgcc cagcctgctg tcaaagtacc
    13801 agccttgact acagaccctg gatcaaaggc gctagtaatc gtgcctaaag cattctcggt
    13861 gatttctttg gaaccttgtt catgactgaa accagtcatt gctagcactt ctcctgtctg
    13921 aggattcata gccactgcat aagcaccagg cgaatactgt gctttgccgt tgttgatcaa
    13981 tgtttgataa ttccttttca aaatttcttc tacttttgat tggaattctg cattcataga
    14041 taacattaaa ttagaacctt tttccccaga gaagatttct ttttgattcg aaacatttcc
    14101 ttcattatca agggaaactt catattgggt tttggttcct tgcaggacat cttcatattg
    14161 tttttccaaa taactttgtc ctacacggtc attccgagaa taaccttttg ctaggtattt
    14221 ctctgcttca tctgctggaa gtccttgctt ttcagtcgtc acactgccaa gaatactttt
    14281 cagtgaactt gctgcattat attctcgtgt ccagtctgtg ccagtcgaaa ctcctggaag
    14341 ttcagaagcg cgttctgcca caacagctaa ctcttgatca gtaacaccag agtttttgat
    14401 atagacagtg tttaattcat atgcactatt catccgttta aagatggtag caatttttaa
    14461 ttgctcctcg tttaaattaa tctcatcagc tgtgacttta ttgaccatcg cactatattg
    14521 ttcgctgttc cctaattgct tttcttttgc cgaaagtctt tttgtggctt ctttcaaatg
    14581 tttgggatca gccagccaaa aatctttttt atctctatct gtcagatttt catctaccgg
    14641 aacatcgatc agcgagttca gcttcgttgc gatttcaagc aaatctttag ccgtaacttg
    14701 tgttcctctc gtatatgtga tggcctgatt cgctttattc gtaactaaag cttttcctgt
    14761 ggagtcgtag atcattccgc gaggagtact ttcttggacc gtaatcgtag aagtagactt
    14821 cactcgcgcc tccatctctt cgccgttcac gatctgcaaa taacccaatc tagcaatcaa
    14881 cgcaacaaat aacgtaaata taatgaaaaa aagaaaattt aagcgaaaag gaacatggga
    14941 ccggcgaaac gttttgttct taggttcttg tgcacctttt cttatttttt ctaaccaaga
    15001 agtatttaat atttttttca taaaatcttt catcatttcc tatccttccg cctatttaca
    15061 ttcatgcttc attgtaccca aaaacttctt aataaacaag aagtatttaa tatttttttc
    15121 ataaaatctt tcatcatttc ctatccttcc gcctatttac attcatgctt cattgtaccc
    15181 aaaaacttct taataaaaaa gaaaaaatgg gcggagcaag aaattcttta cctaatttca
    15241 agataaaatc aactttttgt ttcacagcaa tcacttaaag ttacatatcc aactattttt
    15301 ttcattaaaa aataaactta tttttgaaaa ttatgttttc ttaagtcgtg aaacactttg
    15361 atggatcatt tatttgtgga aaactacgtt ttgttattct aaaaaactta tataaaaaat
    15421 agtaaggttt ttatttcgaa tccataaaat attcttggat tgcttctcgt gtttttttca
    15481 ctttttcgct aagcttttcg tttttagcca tatagaaata aaaaatgata tcgatcgtag
    15541 caaattgggc caaaagtgaa ttagttgccg cacttcgatt cgtgatttct tttggtctag
    15601 atgtttgtac gagtacatcg gctatcttgc taagcgggtt atttccaaat tgcgtcaacg
    15661 caatgatttc gatattcatg cttttcgcca gctcggctaa tgcgactaca tctgcacttc
    15721 tacctgagtt tgaaacaagc caaagcactt tctttttctc attgctgaca agttgcggga
    15781 gcaagacgtg gatatctttt tcaaaaatga ttggcttacc gacacgagac catttttgca
    15841 gaatatcttc tactactaaa gaagaggcgc caaccccata taaaaaaaca gtctctgccg
    15901 attccaaaca ctctacgact ttttggatcg tttcttcttc tagaatatct actgtttctt
    15961 ttatcgctac ttgtgcattg ttaccaagtt tgtttttgat agagtaaaag gactcatttg
    16021 cttctatatc actaaaacct tcatcgattc cagtctgtag ttctgttgaa atcgtgattt
    16081 tgaagtccgt cagactttga aaaccgattt ttttggaaaa acgtacaact gtcggcgcac
    16141 tcaacccgga agcttctgct aattcattag ctgtcattgt agggaccttc tcaatattac
    16201 cgataatgta gttggccaac tttttttctg ctgaagaata atcttccatc atactttcaa
    16261 tacgactaat tacgctcata agttcctcca cccttttaat gatagtctat gtaagtatac
    16321 catatcagac ccttattcac ggtcgtatac tggataaaaa acaaaaaaac aatccgccca
    16381 acagaatatc ttatcccatc aatcccacag attctattga gcatctgtgt ctttgaggaa
    16441 tacctgttat ctattgttcg gattgctttc tatctactac ttttttacag gaaatttacc
    16501 tattagtttt ctctgctgta atttttgact tctgcttcca tagcatcaat aaagatattc
    16561 aaatcttcta cagctgtttc tttacttcca taacgacgta cgttcactgt tgcatcttcc
    16621 aattccttgt ctccaacaac taattggtaa gggatctttt gtgtttgaga ggcacgtatc
    16681 ttatagccca ttttttcatt acgatcatca acttctacac gcaacccctt catttgcaaa
    16741 cgttctttga tttcataagc ataatcgcca tgtgcatcaa cagaaacagg gatgattgtt
    16801 gcttgtatag gtgccaacca tgttggaaaa gcgcctttgt atacttctgt taaataagcg
    16861 acaaaacgtt ccattgtaga aacaattccg cggtggataa ctactggacg atgcgtattt
    16921 tccccgtctt ctccaacata tgtcaaatca aagcgctctg gcaataagaa gtctaattgt
    16981 atagttgata atgtttcttc tgtaccaaga gctgttttga cttgaacatc tagttttggt
    17041 ccgtaaaagg ctgcctcgcc ttctgcttca aagtaatcta gttctaattc atccatagcg
    17101 gctttcagca ttgcttgggc tttttcccac attgcgtcat catcaaaata tttatctgta
    17161 ttgtttggat cgcgatagct caagcggaaa cgataatctg taatattgaa gtcagcatat
    17221 actgccacca tcaattcaag cgtacgttta aattcatctt tgatttgatc aggacgaaca
    17281 aatgtatgac catcattcaa cgtcatttct cgtacacgtt gtaaacctga caaagcacct
    17341 gatttttcat agcgatgcat cattcctaat tcagcgatac gaattggtaa ttcacgataa
    17401 gaatggatcg tgtttttgta gaccatcatg tgatgagggc agttcattgg gcgaagaaca
    17461 agcatttcgc catctcccat atccattggc gggaacatgt cttcttgata atgatcccag
    17521 tgtccagatg ttttgtacaa ctcaacatct cccattattg gtgtatatac atgttggtaa
    17581 cctagactaa cttctttgtc tacgatataa cgttcgatcg tgcgtcggat tgttgcccct
    17641 tttggaagcc agaatggcaa accagaacct acttctgggg aaaccatgaa caagtcaagt
    17701 tcttttccta gtttacgatg gtctctttct tttgcttctt cacggagacg gatatattct
    17761 ttcaaagctt tcttgtcaaa aaaggctgtc ccgtatacac gttgcatcat ttgattgttt
    17821 gagtttccac gccaataagc acctgccact gataataatt taaatacttg tatacggcca
    17881 gtggatggaa catgtggtcc acgacacaaa tcgatgaatt cgccttgttg ataagcagta
    17941 atcacttctt cttctggcaa ttcactaatc agttctactt tgtacggatc acttgcaaaa
    18001 atttctaatg cttcctgttt tgaaagaaca cggcgttcga tcggcagatt ttctttgacg
    18061 attttcatca tttctgcttc gattgccggc aaatcttctg cagttacttg gttttgtcca
    18121 ttatctgtat cgtagtaaaa ccctgaatcg atagctggtc caacgccaaa gtggatattt
    18181 ggatagaggc gacgcatggc ttgggccatc aaatgagctg ctgaatgacg gactaagcca
    18241 agtgcatctt catggtctgg tgtgatgatc tcaatacttg catcttcctc gattggacga
    18301 gtcaaatcaa cgagttttcc gttcacttta ccagctaaag ctttttttgc taagcttttt
    18361 gaaatgcttt cagcaattgc caatgttgtt gttccagact caaattcttt gactgcacca
    18421 tctggaaaag taatttttat cataatagtt tcctccttga aatgttttga ttgatcagat
    18481 catcaagctg aaaaacaaaa aaatcctagc atctcttgaa aagagatact aggacgaaaa
    18541 gttcgtggtt ccacctaatg ttcaaacgca aatgcgttcc tcaagcgttg taacggtccg
    18601 accgcccttg cttcaacaag ggtttcgaaa gtggtcatcc tttggttttc tcctggaaaa
    18661 gtttcagcct agcttttcct ctcttatcga agactctcaa attcagttgg ctttctcatc
    18721 aatcaattat gtgtttattt aaccactaat aaatagaaaa atcaagtggg aaactaaact
    18781 tattttttcc attccccatt tacaccgtat gttccttttt tcatctcatt gatgatgaca
    18841 tggatgtgtt cttttggtgc ctgcgtgttt ttatgaacag cttccgtgat atctttcatc
    18901 atattttcta attgttccgg actgcgacct tctactaatt cgacatgtac gaatggcata
    18961 atgctctctc ctctttctga tcgttctttt ttcaactcga ttctatcata ctatgtttgt
    19021 cttgggtact gaatcaggaa aaatggctgg atcttttttt gctctcaagg cactcaaaat
    19081 agagaccgta tccacgactt cttgcaaaac agctccgaat aatgcgggga ttagtcccgt
    19141 acttgcaatt aacatcaaaa aaatacaaat aaagatgcca atcagtacag actgtttcgc
    19201 tattctcatc gtatctcttg aaatcactac tgcttttgct acacgcaaga gatcatcttt
    19261 caagatgacc gcatctgcac tttcactagc agctgtagaa ccatgagccc ccattgcaat
    19321 accgatatct gcagcagcaa gtgctggtgc gtcattcacg ccgtctccta ccatcgcaac
    19381 aggtctttga tctttaggga tactttctaa aatggtcagt ttatcttgag gcaagctacg
    19441 ggcatgtaca gaatctaatt ggactttttc tgcaatcgtt tgagcaatgg cagatccatc
    19501 cccggtgatc atcatcaaat ttttcacatc aagttttctt agctccttga ttgtctttgc
    19561 tgcttctggg cgaatctgat ccgaaaatag gatcgttcct gcaaaagatc gatcaattgt
    19621 tacatagatt gctgtattgg acgtttctat ttcttcagct gaaacaaaag aggcacgtcc
    19681 aatctttact tcatgattat tgactactgc ttggattcct tgaccggtca cttctttcag
    19741 ttgactaaca ggatacagtt ggctgttttt cacgttgttc actaacgaac gagctaagat
    19801 atggctagat tcctgttctg cactagctgc atacagaagt agctcttctt tttttattcc
    19861 attgactggc tgaatcgcct ctacttccaa tatccctttg gtaagcgttc ctgttttatc
    19921 aaaagcaatc gtttttgtac gtgcgagctt ttcaatcgtt gttccagttt ttacaacgat
    19981 cccgttttta cttgaacggc tcattcctgc aactaaagca atgggcgcag ctagaatcag
    20041 tggacaaggt gatgccacta ccagtacctc agcaaatcgt aaagggtctt ttgagaacca
    20101 ccaagaaata ccaccgatca aataagcaat caacgtgaaa gggactgcat aacgatcagc
    20161 taaacggaca aatcgggcag gcttttcttt tgattcttcg actagtttaa taatcatttg
    20221 atactggctg tctgcggcac gtttttcagc gatcatttgg atcgatccgt ctccatttaa
    20281 agaccctgac ataatctgat ctccaggttt cttggtttcc gggcgggact cacctgtcaa
    20341 agaagattca tcgaccgaag attctccttt ggtcactcga ccatctgcgg gtacaagttc
    20401 gccaggcttg actagtaact ggtcatcaat atttatttca tcgattacta tatcctgcaa
    20461 ttttccttct ttgaatcgat gggcaattct tggggtgtta tctaacaaag actgcagttc
    20521 tttacccgct cgtttattcg cgtaatcctc tagactatct ccgcccgtaa gcatgatcaa
    20581 tatcatcaaa ctagcccagt attctttgac ggctaatgtt gcaaggattg cggtaatcgc
    20641 taatacatct acgccatatc ttcctgaacg taacgtcttg atcatttcca aaaacatcaa
    20701 gaaggcgaga atccctccag taaatgcgac aatcgcaaat gcaagggccg gctggtgaaa
    20761 aacaaattca cttaataaag ctatcccacc aataagcaaa gtaagtaaaa attttttgaa
    20821 atgtgtcatt gttttttctc ctcttttatg tgacttagag aggttagtaa taaatttcca
    20881 gctgcctctt tcatcttaag catagtcctt gactagcact ttttaaaaca ataggcctta
    20941 attgaaaaca actatcacta tcagcgtttc caaaattcat cagttctaca aacaaaaata
    21001 ttcagcaagg attacttttt cgattcatca gttaaaaagt accttttttc gaaaaactat
    21061 gctatacttt taattcgtta tttacggcgg aaggagcaaa catgcgatga atgagaaaca
    21121 actagaacaa acgcacgtgg atgaaacgat cgaactaatc cagatcgaac aaaatatgct
    21181 ggatacaaaa caacaagaat tgactcagga aatgaatcat tcgacaaaag acacagctaa
    21241 tcacaagatt cgagctggtt ccaatgaagc tttttatgaa tctgctgttg aatatcgcca
    21301 acatgagcag gaattgcttt tgaaatacca tactcttgaa gcacagcaaa aacgcctgca
    21361 aacgttgtct gtcatgaaag atagtcctta ttttgcccgt atcgacttta aagaagaccg
    21421 tgaaacagaa acattatatc taggtatcgc ttctttaaga gatctggcag aagaaacaat
    21481 cgttattgac tggcgcgcac caattgccaa tctttattat gaaggtgaga ttggtcccgc
    21541 attttatgaa actgaaacag atagaataga tgtcgatctg cagttgaaac gacaatttaa
    21601 aatccaagaa ggacgtattt tatctatggt agatacgtcg gaagtaatca atgatgactt
    21661 tttgctagag atattggatg aatcatcaag caaccatatg aaaaacatcg tctctacgat
    21721 acaaaaagca caaaatgcaa tcattcggga cacaacaagt aaagtaatgc tgattgaagg
    21781 aatcgccgga agtggaaaga catccgcttt gcttcaacgt attgcttttc tgttgtatca
    21841 taatcgcaag tggctcgagg ctgaaaacgt tcttctattt tcacccaacc atttgttttc
    21901 tgattacatc tcgactgtat tgccttcatt aggtgaaagc ggcgtgccta cacagacatt
    21961 caaaaattat ttggtgcagc tgctgccaga gttttcttta ctagatgaac agcaacaaga
    22021 aagtggtttt ttgctagggg aaaaagatcc gatacaagtg atgaaaagcg gattgacctt
    22081 agtcgatcag attgatcgct atatccaagg catcacaaac tacggtccct tgttccgtga
    22141 tatgaaaata aatggccgta cgatcctatc aaaggaatcg attcgccaat ggtataaaga
    22201 aacaaatgaa caattacctt tacatcatag actttctcta ttacaaacga agctcctgaa
    22261 aaaattaggc ggtctgcaaa aagacgaaac acgtcagcaa tgggtcaaag atctggcgga
    22321 agaacagctc caagaactat acgcaacaga tccaaatctt gaatatacag aacaaaagga
    22381 gcgttcatta cgtaaaaaac tggccaacca aatcgtacgt aaaagattcc gtcgaatcca
    22441 tcgagggatc atgaattatc aatttgtcaa tataacgaaa caatatctcc actttctaca
    22501 aactgtgcca ctttctcttc taacacgaca taaaatcagc gagaccaggt ggcaggaaca
    22561 tttgacaact gttcgtacac acttgcgtca aagagaattg cgtcaagagg atgctgtact
    22621 attcttttta ctgatgcgcc gaacatatcc agtgacggtt acacacaaag ctcgctatat
    22681 ctttattgat gaaatgcagg atttcgcacc atcacaagtg gcattgctgc aagccttgtt
    22741 cccaaaagca aatttgactt tctgtggaga tctaaatcaa aatgtctttg gaaatgaaac
    22801 gatcgttgga tcattggaag accttttcgt ggaccgtgaa gtcactcgtt tccaattaac
    22861 gacaagctat cgctccacaa aagaaatcac tgattttgct aatcatttta ttgtgaaaga
    22921 acatcaagta gaaacaacag caagaaaagg agaccttcct ttgctcatcc aaggaacaac
    22981 agatgatatc aaattgaatt ggttagcaga taccattcga acgactcagg gaaaagcccg
    23041 gtattggcgt acagcaatca tagctaaaac acaagaagaa tgcgaagctc tttatgctca
    23101 actgccagac gagttgaaac aacaagtcca gctgatttta aatgagagtg actttatgaa
    23161 gcgttccatt attttacttc cagccttttt agcaaaaggt ctggagtttg accgagtttt
    23221 cttgtgggat gtagatgcag aacatttttc ttctcagcaa gacaaattga tcctttacac
    23281 catgtgtaca cgtgcgatgc atgaattgac cctttttacc tccaataatg aaagtccttt
    23341 gttaaacaat atggatattt catcttatga gaaaaaagta ctgtaagtaa aaagacaact
    23401 gctatcatta atgttagcag ttgtcttttt atttacaata ataattgcag aaaaagtaat
    23461 tcaatcattt aaggtcattt attctatata taaatccaaa tctgtttcag gatattttaa
    23521 tttgacaagc gaaataatca ttttcagttc ttccatgttt tctggatatt ctgggtcaaa
    23581 taccttgcta tgacggccaa tcccgccatt gtcgatcaat cggtaaattc gattccattc
    23641 aatgaattgg tcaattgatt ggaaattgct gattggatag ccgccacaat ccaaaatctc
    23701 tgatacacga aatgctaggt atgcactatc gatcacttta ttttttttcg tgtctatctt
    23761 ctcatttctt tcgataagaa ttttttcagg acgatgtttc aagctccact ctaatagtag
    23821 aaagaaagcc tccatccgat actcttcttc aaataaccga aaggctgaat cagaacgacg
    23881 aaaaagcagc ggaatcagat aattttgtct ataaatcagc attggtgcgc ggtccaccat
    23941 acactctaaa taacggttca gataccctgt accgacatag tttcctaatc gatagccttc
    24001 aacgccgaat cttgcgtatt tttgcgctaa ttttgaacaa tcagttacgt tcacagctaa
    24061 ctcttctact ttgcggtaac cgcttaagat acgttcaaaa ggttctaaag tgatttttgc
    24121 ttccatattc ttccccgtcc atcttccaca tacttcatat catttcacat gatacttatg
    24181 ctctcatcat accacaggtt gaattagaaa agaaaataaa tcacacaaaa aagcaagaca
    24241 atctgttagt gagattgtct tgcttttcga aaaagactga atacttttcg ttaaaactgg
    24301 ttgcttagtc ttctaatgcc acattatgga acacatgctg tacatcttct agatcttcca
    24361 gtgcatcaat cattttttca aatgtttctt gatcttctgg agatagtgtt acctcgtttt
    24421 gagggatcat ttggatctca gcgattgaaa aatcagtgat tcctttttct tttaatgctt
    24481 cttgtatact atggaaatcc gctacttcac cgtaaacgat gatttgtcct tcttcttcca
    24541 ttacatcgcg aacatcgatg tctttttcca ttaggtattc aaggatctcg tcagcgtctt
    24601 ctcctgcaaa agcaaagatt gctgtattgt caaacatgta agaaaccgct ccggaaacac
    24661 ccatgttccc gccatttttc ccaaatgctg cccgtacgtc tgctgctgtt cgattcacat
    24721 tgttcgtcaa tgtatcaaca atcaccattg agccgcttgg tccaaatcct tcatagcgca
    24781 attcagagta tgtttcatct cctgcacctt ttgctttttc gattgctcgg tcaataatat
    24841 gttttggtac attataagtt ttagcgcgat caatgacgaa acgtagtttt tggtttgcat
    24901 gcggatcagg atcacctgat tttgctgccg cgtagatttc aatcccaaat tttgcataaa
    24961 tccggctatt attagcatct ttagcagttt tctttgctac gatatttgcc catttacgtc
    25021 ccatattttc acttcgcttt ctgatttgat agtttcataa cgcaaaaacg tcctgttcca
    25081 ttatacttgt gaatcacgat tctgtgaagc tttttttctt tcttccttca ttcttctcat
    25141 ttttagtcaa ttatctatac tcggattggg atttgcccga aattctgttc cggaatattg
    25201 cctcccaatt gatagatttg atcacttcta actaacagtt ccgcttcttg tgcctctttt
    25261 tttccgattc gggaataaat agaaatctct tttttttctt ttaagaatct tcttccagca
    25321 tcggtaaatc caagtagtcg aagataattt ttattttgtt ggacaggaat ctcttgcttt
    25381 ttgatattca gcaagacata acaagataat cgttggatcc gtgtccatgt atatcttttt
    25441 gtttttacct ttgacacaaa atcctcaaaa ctatcagcag ttgcagctgc tgttttcatg
    25501 cgcgattcta ttccttcggt catctgatag atatctgtca gttccagcaa agtagaagat
    25561 aacaaccgat aacgcaaata cggccaataa gattcccaag tctgcagcga acaagttgaa
    25621 agatcttctt ctgtctgagg cggcacaagt ccagctattc tttttccttc ttctaacgca
    25681 tggcgaatgg ctgttgcact ggctatcctc tgatctgtga tgattttttc atgatagcct
    25741 gcacttttac gtcggatcgg atgtaattcc atagggtgga catacttggc attttccttt
    25801 gcataactta atcccaagat atgatttggc gaagaaaaat caagcgtaat atcgggatac
    25861 agactgcgaa aaacctcagt cattttttgc ggataactat acgtttgatc agacagttct
    25921 tgaaatgcct cttctatcct atcttgattc tcttggacaa agcgtccgaa actgctgtaa
    25981 tcaaacgaag tctcttcttc tgttccaaaa cacaaactgt cacagtccag tgtttgcaag
    26041 atttttactg cacctttagc aaaatagtca gctgactgaa ccgcccattc gacgggaagc
    26101 tccactacta aatccacacc attttgtaaa gcttcattcg cgcgatgcca tttatcgatg
    26161 atagccggtt cgcctctttg caaaaaattc ccgctcatta cagcaatgac gacctctgct
    26221 cctgttattt ttcgtgccat ttccacatga tagcgatgtc cattgtgaaa aggattgtac
    26281 tctacgataa tcccgcagct tttcatgcgc atcctccttc atagttatcg ataaaaaaga
    26341 aatagagaca agtaaaaaaa ggcaaaaatc ggaaaaaata cccgaactat ataaactatt
    26401 ctcgcaaaca caatacttct gcgacgaaaa gctgcactgc aagagcaatg cctatatcgt
    26461 tcggatatat aatttatgtt gaagatttat tcttttcttg ttaactcgag caggcaaaac
    26521 cgctagtctt tgtatgctac aaagaaccag cgctttgatt tctctgatgc tgcttcgtct
    26581 gtaaaatcag aaaatgcttc gactttcgaa aagccgctgt tttccagcat tcttaaataa
    26641 ctttccaatg gataggtacg ctcttgatgt agttcatctt cacgaataaa tcgaccagac
    26701 ccttcttgtt cttcaacgaa aaaggtaaga aaatgttcga tgctgtgttt ctcgcttcct
    26761 ggataactat cccacaaaaa agcaaaatta tccccttgat aatgatagct atattctgga
    26821 aaaacctcat ctacttgata ggtggagtgt acgtcaaata aaaatcggcc attttcttct
    26881 aacgcttgat agacttcatc aaacacttgc tgtacctctt gtctattttt catatagcac
    26941 agcgaatcag aaaaacaggt aattgcctga tattgtccga tttctgataa atcgagcata
    27001 ttgccttcca caaattggat cgatgcgtct tcttcatatg ctcgctggct ggcaatcatc
    27061 aacatctctt ctgaaaggtc taaccctgtc actttatatc catcttttgc aaaggcaacc
    27121 gctaatgctc cagtaccgca tgccagttcc aaaatatttt ttgtgtcttc ttgtaaatga
    27181 cgtttcgaaa aagctaacca ttgctgataa agtgtttcgt ccatcacttc atcgtaaatg
    27241 aaagcaaatg tttcataagc catatgttaa tccaaccatt cactaagatc gactagcgga
    27301 gcatctgacc atagtttttc cagattatag aaatttcgtt ctggcgcatg gaaaacatgt
    27361 acgatcacat ctccaagatc gatcaatacc cattttccgc cttcttttcc ttcaatacgt
    27421 tttacttcat atttgttttc ttcttcttta tcaatgattt cttctgtaat tgcgttgatt
    27481 tgacgttcgc tatttgctga acagatcatg aaataatctg ccaacaagga aacatttcga
    27541 acgtctaaag cgataatgtc ttctgcccgt tttgaatctg ctgcttcaat cgcaattttt
    27601 aattgttctt tactatctat gatgatttcc tcctaatatg agcagcttat ttttttgcta
    27661 cccagtaatt atatgtttcg attgtttttg gataaatctg ttgttcttgc gcaagcaaat
    27721 gggccaatgt atgttttgtt tcaaatgcta cagcttcgtc tagatcagcc catgcaatgg
    27781 ctcgtgcatc ttgtacccca ggaaaatctc gacctggttc aatatagtct gctacataaa
    27841 tgattttgtc aaggagactc atcttagcag ccccagtcgt atgcacccgg attgcatgaa
    27901 gaatctctgc atcggtgatt cctaattccc gctctacaaa cgaagcgcca acaagaccat
    27961 gccagattgc attattataa ttcaataact ccggatcaaa accgtctctt acgattacca
    28021 ttttgaactc ctcatctggt cgttcttttg cataatcatg tgtcaatgca gcaatactag
    28081 ctttttcagg agaagcaccg tattttttgg caagagccac ggctgtttct tctactccaa
    28141 gcacatgctt gaagcgttgt tcgctcattc tcatctgtat tttttgcatc aggttttctc
    28201 tagaataagc agtatattca ttactatagt ccatattcat acaacccttc attttgaata
    28261 taatcaatta ctttatctgg aacaaggtag cggatagaac agccttcttt gatcttttga
    28321 cgtattttcg tggaactgat gtcgatctca ggtacatcta cccaaattac aggatatgga
    28381 gtatccgttg tgtatcctgg tcgacgtata cccacaaagt tgaccataga agtcagctca
    28441 tcgattttat accattttgg caaatattct accatatccc caccgataat aaagtaataa
    28501 tctgtatccg gattgttttg cgtcaattcc ttcatagtat cataggtgta acttttccca
    28561 ccacgagcta attcaatcgt ttcaatctgc aaaaaaggat tgtcttccac cgctaattcc
    28621 aacatgttga gtcgatgttt tgcatcaatc gtctgtttct catcaacatg aggtggctga
    28681 taagaaggca tcaaaaatac gcggtcaaga cctaaattcc ggccagcttg ttcagccatc
    28741 actaaatgtg caaggtgaac gggattgaat gttccgccca agatccctac ttgctttttc
    28801 ttctctaaga attgaggcat ttcttcagga aataattgtg aacgaacaaa tgtcttagct
    28861 tgcgtattca tcatggtcac tccttatatt gcttttactt cttttgatag cctttgatac
    28921 ttttcttttg tggaaggttt gtataataca ataacacgtc caatgatttg gacgatttcg
    28981 caatctattt ctttttctaa tacttgagct gcttcttctg caatctcatc tgtattttga
    29041 agtaacgata ctttgatcag ttctcttttt tctaaagctt cttcaatttg aacgatcatt
    29101 gctgaattga tcccaccttt tccgatttga aaaatcggct gaagatgatg cgctttgcta
    29161 cgtaaaaaac gcttttgttt tcctcttaaa ctcataattt tccttcttcc ttaaatcaat
    29221 gctttgcgtt tcaatacatc tacacccttt ggcgcccagc ccgcaattac tactggcgct
    29281 gttacagtga tccagcctag accggcaaaa acgatgtctg ttttttcttt cacagaaaac
    29341 tcaaaacgaa caagctccgg aaatgcctcg acttcatcag gacgaggagg ctgtaataat
    29401 ccacctacat gtttttgata aaattcgtct gctttttcca gtttcgttcg atgaatattt
    29461 acatcatttg acacataagc aataaatgaa gcacgattgc cgctgataaa atcaaatctt
    29521 gcaagtccgc ccaagaaaag tgtttgccct tcattcagct gataaacttt aggtttgatt
    29581 tcttttaacg gtgcaatgat ccgcaaatct tttttaccta agtagtgcgc catttgatgg
    29641 cgatggataa ttcccggtgt atcaatcaaa aaatgcccgt catctaaagg aatctcgatt
    29701 ttatccaagg tcgttcctgg aaactgcgaa gttgtgatta catcttgtac tcccgctgta
    29761 tttttgatga tttgattgat aagcgtcgat tttccgacat ttgttactcc gacgatatag
    29821 acatcccgtc catcgcggta ttcttcgatt ttttctaata gttccgtcat ctcatgagat
    29881 ttcttagcac tagttaataa tacatctact ggacgaattc ctgcttcgta agcacgttcc
    29941 ttcatccatt gtgttaattt agtttttttc aatgatttag gtaatatatc cactttgttc
    30001 ccaactaaaa gtactggatt gtcaccgaca aaacggtgta gtcctgggat tagtgaacca
    30061 ttgaagtcaa aaatatccac cacattgacg atcaatgcat ctttttgccc taattcattc
    30121 agtaagcgca aaaagtcgtc atccgtcaat gagacatctt ggatctcatt ataatggcgt
    30181 aatctgaaac aacgctgaca atagacttcc ccggaagcta aacctttttc caaagccgtt
    30241 tgcggcgtgt atcctaattc accgggtttg tttgtttgta taacggcacc acagccgata
    30301 cattgtaatt cttctgtcat tcgattccac ctcgccagcc catatcaggg tgttttgcta
    30361 atagataggc cataattctt ttttcccaaa aacgattaaa ttgtgtcttc caactatctg
    30421 ttgcgataat gggttttaca aggatgctac gtatccctgc acgattggct ccacgtatat
    30481 cagtcataat ctgatcgcct accattacta tctcatcatc tgctaaatcc aattgtttct
    30541 ttgctatgtt gatccctctt gctaaaggtt tcattgctcg tgcaacatac aaaagatcaa
    30601 atttttcaat cgctcgcgcg acacgttttg aattattgtt tgaaacaaca acaacaggga
    30661 ttcctgcatt tttcatttct aaaatccaag taagcaactc ttctgttcca tctggattat
    30721 tccatgcaat caacgtattg tctaaatcgg tcaatactgc tttgatgcct agattcttta
    30781 attgggcagg tgtaatctga tagatggctt ctaccatcca tgttggtttg taatttgaaa
    30841 acatgctgtc tcctcatctg caaaaaatga aggagcacat aaagtactcc tcaaccccgt
    30901 attatactgc atatcagcaa aaaaaactac ctctatactc gttcgtcttt cagaaaaagc
    30961 atttctttac aaaatgacca aaaataaaga ttgatcacag aaaaatttag aaacaaaaaa
    31021 caactaatat tggcggaacc tcttgtatcg tttttccagt aatttatgct gatctaatcg
    31081 aacaagttcc gtcaatcttt caatcaacgc ttttttcata atgtgtgcga ctttatatcg
    31141 tggcaactgc tcgccgttga acgtttcagg aataactcga tcaacgattc ctaactcgcg
    31201 tagttctggc gcagtaattt tcatcaactc agctgcttct gcggcgcgac taccgtcttt
    31261 ccacaaaatc gacgcaaatc cttctggaga aagcacagca taaatcgttt cttccagcat
    31321 ccagacttca tccgctaaac ctagagcaag ggcaccgcca cttccacctt caccaatgat
    31381 gatggcgatg atcggtacct ttaaatcaga catttcgagc agatttctgg caatagcctc
    31441 tccttcgccg cgctcttctg ctcctacacc gcaatatgca cctgctgtat tgatcaaggt
    31501 aatcaccggt ctattgaact tttccgcttg tttcatcagt cgtaaagctt ttcgatagcc
    31561 ttcaggatgg ggagaaccaa aatttctgcg gatattttcc tgcagatccc gtcctttttg
    31621 aataccgatc actgttaccg gtttgccatc taatcgagcg attccaccaa caatcgcttc
    31681 gtcatctgca aataaacgat caccatggca ttcctcgaaa gaatcgaaga tctcttcaaa
    31741 aaaatccaag cttgtccatc gttctttagc tcttgctaac tgaacgacat catgtgctgt
    31801 cttatccacg atctatccat cctttctgtg tatgcaattc aagaagcgta tggattttct
    31861 gtttcaattc catgcgggga acaatctgat cgacaaaacc atgagataat aagaattcag
    31921 ccttttggaa atcttctgga agttcctgct tgatcgtctg ttcgatgacg cgtcttccag
    31981 caaaaccgat caagctttgg ggttctgcta gaataatatc gccttccatt gcaaaactgg
    32041 cagttactcc tccggtcgtc gggtccgtta aaacagttag ataaaaaaga ccttcattgc
    32101 tatgccgttt gacagcagca gagatttttg ccatctgcat tagagaaaag attccttctt
    32161 gcatccgtgc gccacctgaa gctgtgaaca agacgactgg aagactttcc tttgttgctc
    32221 gttcgaataa tctggtgatc ttttctccga caatcgttcc catacttccc atgataaaat
    32281 taggatccat cactccaata atgaagggaa tcccctgaat ggttgcttta ccagtgagga
    32341 ctgcttcatg aagtcctgtt ttatcttgca tttttttaat tttttccgta tattcgggaa
    32401 atgctagcgg atctttggtt acaagatccg tatcccattc ttcaaaactt ttttcatcaa
    32461 taatcaaagc tagacgctgc catgcaccga ttcgaaaagc gtatccgcaa tgagggcata
    32521 ctttttcttc accgatgtct ttcgtataaa ggatatgttt gcaatttgga cactttgccc
    32581 acatgttatc aggaacagat ggcgagttag caggtttgct ctctctatta ggattgatcc
    32641 gaatatattt ctttttctta aacagagcca tctatgcgcc tcctatctcc gtttctggtg
    32701 tccaattcgg taaaaattct tcttgtaaaa atgctgtgct gtaatcacct gctatgactg
    32761 caggatgact aatcaaatcc atctgaaatt ctgcgttcgt gatgattcct tctgtgataa
    32821 attcacttaa ggctcgctgc atcttcatta atgcttcaaa acgcgtattt ccatggacga
    32881 ttatttttgc aatcattgaa tcataatatg gcgggatcgt atatcccggg taaactgcac
    32941 tgtctactcg cagtcccatc ccacctgctg gaagcagtaa gttttggatc ttccctggag
    33001 aaggagcaaa atggaaagca ggattttctg cattaatccg gcattcgatt gcgtgtcctt
    33061 ggaatactac ttcttcttga cacacattca aaggctcacc ggctgctatc tccacctgtt
    33121 tttttactaa atcgattcct gtgaccattt ctgttactgg atgttccact tggattcgag
    33181 tattcatttc catgaaataa aaatcgcctt cttcatccat cagaaactcg atcgttccgg
    33241 cattttcata attgactgct ttagctgctc gaacagcagc gtctcctagt gcttctcttt
    33301 ttgtttgtga gatcactaca gaaggagatt cttccagtac tttttgattg ttgcgttgta
    33361 aagaacaatc tcgctcgcct aaatgaatga catgaccaaa tcgatcacct aagatctgaa
    33421 cttctatatg gcgagcagga taaatgattt tttccaaata catatcatca tttccaaatg
    33481 ccgctgctgc ttcttgctga gctgaagaaa aatgctgtgg gagctcttct tttgattgaa
    33541 ctttccgaat gcctttcccg ccaccacctg cagctgcttt cagcatcact ggataaccaa
    33601 ttctgtctgc tatttccaat gcttcttcta ctgtagataa tacaccagta cttcctggaa
    33661 tgactgggac atttgcttct tgcatcaatt gacgcgcatt gatcttattt cccattgcat
    33721 cgatcgttgc acttttagga ccaataaatg tgatattgca ctcttcacac atggaggcaa
    33781 atcggctatt ttcagataaa aaaccaaatc ctggatgaat tgcttcggct tttgtcacga
    33841 ttgcagcgct cagtacttcc tgaacattca gatatgaatc aacggctttt gccgggccaa
    33901 tgcagattgc ttcatctgct aactgtgtat gaagtgcttc ggcatctgct tctgaatata
    33961 tcgctacggt ttggatccct agttcgcggc atgcacgaat gatacgtaca gcgatttcac
    34021 cgcggttagc tatcaatact ttagaaaaca tatttcacct atccaatcat aaaggttaat
    34081 tccgcttcgg ctactttttt accgtctact ttagcgattc ctttgcctat tccggctgaa
    34141 gctttcactt tcaatatctc aacttcaagg tataaggtat ctcccggtgt aactttctta
    34201 cggaattttg ctttgtccaa tccgccgaaa taagccgtct ttcccttgaa ttgttcaaga
    34261 gaaagtaatg caactgctcc tgcttgagcc attgcttcaa caatcaatac ccctggcata
    34321 acaggttctt ctggaaaatg tccttgaaaa aagggctcat tgatcgttac atttttctta
    34381 gcaacgatcc gttctccttc aaccatttct tccactcgat caatcaaaag cattggataa
    34441 cgatgtggaa tgatttcttt tatttcttgt atgttcattg ttttccctct ttcctgcttt
    34501 gcttattttt gctgaatcct aaagagtggc tgtccatatt cgacaactga ctcgttttct
    34561 acaaggattt ctgtgatcac tccgtcggcc tcactagtta tctcattcat cagtttcatc
    34621 gcttcaataa tgcagataac ttctcctttg gcaacttgat cacctacttt tttgaaagct
    34681 ggtttgtcgg gcgctggttg cagataaacg attcccacga ttggcgaaac aacctcgatt
    34741 ccatcaaata ctgccgattc tttttctttc attatttctg gagcagttgt tgcttgttca
    34801 gatatgacat tgactggctc cgaaactggc aacgtttcgt ttgtttgaac catattcatg
    34861 cctcgacttg ttttattttt attcatatac aattcaaatt gaccttcacg caaatcgaat
    34921 tccgttagcg aggattgatc aaattgactg actaattctc tgatttcatt gatatccaaa
    34981 attattcctc ccagcgtttc atgcacagaa ccgcattatg tccgccaaac cctaatgaat
    35041 tactcattgc ataagtaaaa cttgcaggtt tgctttcgtt tttgacaact gtcaagtcaa
    35101 tctcaggatc ttgtttttct acattgatcg taggtgggat aaattgatgt tctaatgcca
    35161 gcaaagttgc caaggcttcg atcccgccgg ctgctcccaa taaatgtcca gtcatcgatt
    35221 tcgtgctgct gacgtaagta tctctgaaat tttcacctaa tgcgatttgg attgcttttg
    35281 cttctgcctg gtcattggct ggtgtacttg ttccgtgagc attgatatat ccgacttgtt
    35341 ccggctgtac accagcttct tccatcgcaa gcttcattgc tttagatgca ccagaaccat
    35401 ctggagtagg agctgtcata tggtaagcgt cacagtttgc accgtatcca acgatttctc
    35461 catagatttt ggcaccgcga gcttgtgcat gttctaaaga ttcaagaact agaacgcctg
    35521 ctccttcacc catcacaaaa ccattccgat tttcgtcaaa cggaatcgaa ccacgattag
    35581 gatcagttga ttctgagagt gctgttaaag ctgcaaatcc agcaatcccc atctcacaaa
    35641 tcgttgcttc tgttcctcca gcaagaatga catcagaata gccatgtttg atgttacgga
    35701 atgcttcccc aattgaattc gttccgctag cacaagctgt tacgatagct gtacaaatac
    35761 cttttgcacc tacacgcaaa gcaatatttc cagctgccat gttggcaatc gccattggta
    35821 cgaacattgg agaaactcgt tttgcacctt tttcatgtag acgaataact tggttttcga
    35881 ttgttggaag accaccgatc ccagatccga tgatgacgcc aaaccggtct tgattcattt
    35941 tttctttatc taaaccgctc atctccattg cttctaaagc agcgtagatt ccatagatcg
    36001 aaaacatatc cattcgtttg ctatcttttt taataaaata tttatcgtaa gggaaatctt
    36061 tgacttcacc cgctaaggta atccctgttt ctgaagcatc aaatttagtg atcggtgcga
    36121 tgccattcgt cccctctttt aagctattca aaaagctgtc tgcgtcattt ccaataggtg
    36181 aagtgactcc atagccagta attaccactc gattcatttg tttgctctcc ttttcttatc
    36241 cgtgcatgac caatccgcca tcgacattga tgacttgtcc ggtgatatat ggattttgtg
    36301 ctaagaaaac tgctgtttgt gcaatatctt ctacttgtcc gaaacgctgc atcggaattt
    36361 gcttttccgc ttgttccttg actttgtctg ctaaaacttc cgtcatatca gttgtgatga
    36421 atcctggcgc aatggcatta catgtaatgc ctctagttgc agcttctcgt gccacagatt
    36481 ttgttaatcc tactacgcca gctttactag ctgcataatt tgcttgacca atatttccaa
    36541 tcaacccaga tacactagag agattaatga tggccccttc tcgctgcttc atcatttttt
    36601 tcaatacgtg ctgcgtcata ttgaaggttc caatcaaatt gatatccaga cattttttga
    36661 aatcttctgc atccattcgc atcacaagtt tgtcatttgt gattccggca ttgtttacta
    36721 acacatggat cgatccaagc ttttcttctg cttctttgat catttgtccc gctttctcat
    36781 aatcagagat gtcaccggaa actcctacgc attttacacc gaatgcttcg atttcttcta
    36841 tcttttcttt tggaatctct ccgcgaccat tcaaaatgat atttgctcca gcttttgcaa
    36901 aagctaaggc catcgctttg ccgattcctc ttgtactacc tgtgataaat acattttttc
    36961 cttttacatc cataacgttc ctccttatag tattgctatt gtttcatcaa atgtctgctt
    37021 attctcaaca cgcagaactg gtattgtttt atcgattttt ttcataaatc cgcttaatac
    37081 ttttccgggg ccgacttcga tgacttgttc gatgccgatt gtttttaact tatggatact
    37141 ctcatagaaa cgaacaggtt gcatgacttg ctgttcaagt agtcccgcga ttgtttcttt
    37201 cttcattatt tccgttgttg tgttgcttat gataggaaaa gaaggttcag agaattgaat
    37261 ctgacttagg tccttcgcca gtttttttgc tgccggttct aaaatagctg tatgaaaagg
    37321 cccgctgaca tttagaggga tcattctttt gaaccctttt tcttttaata gtaaaacagc
    37381 ttcgtctact gctttttctt cgccaccaat cacgatctgc tgaggtgtat tataatttgc
    37441 aggagatacg atgccgtact tgctagcttc atgacagctt tcctcgatcg tttcaatcgg
    37501 cgcattcatc actgcaacca ttttcccgct tccagctggg gtagcttctg tcatataagc
    37561 gcctcttttt gctactaacg caactgcttc agtgaaggat agcgcattac tagctactaa
    37621 tgccgaatat tctcctaaac tcagtcctaa agctgcatct gggatgattc catgctcctt
    37681 caataagcga taatacgcga tgctgactgt tagaatagct ggctgtgtat attgcgtctg
    37741 atccaaacgt tcattttctg taaagcaaag ttcagccata tcgtagccga gaatctcact
    37801 tgcttcatca aatgtctgct ttacaacggc ttcttcatat aaatcttttc ccataccttg
    37861 atattgtgca ccttggccac taaataagaa tgctgttttc atgtttttct ccaatatatc
    37921 cagaatatat gtttgattct tcctattcaa aaaggccgag acattcctat ttcaggaaat
    37981 gtcccagccc ccaacctaat tttaatgatt atgcagtttg tttttctacg taagtcacta
    38041 aatcttgaac cgttttgatt ccgtcttctg tttcgatttt cacgtcaaat tcatcttcaa
    38101 tttcattgat gatttggaat aaatccaagc tgtcagcttc taagtcttct tggatgtcag
    38161 tagtcaattt gatttcttca gtttcttttc ctaattcttc tgcgatgatt tcttgaattt
    38221 tttcgaatac cataataata tattcctccg atattgtttg ctttttattt ttataattta
    38281 acagtctaca actccatgag aatacttccc catgttaaac ctccgccata gcctgtaaat
    38341 attactttct gctgttttcc taaggtgatc tttccactag aaactgcttc atccaataag
    38401 atagggatgc ttgcagcaga tgtattccca tatttatcca tattggtcag gaacttttct
    38461 tgcggcatct ttacttttcg ggcaattttc tcaataattc tgaggtttgc ctgatgcaaa
    38521 agcagataat caactggttt gtctttcatg acttcttgga tattgtttgc aacgtctcgt
    38581 acggcaaaat caaaaatatc tctgccagtc atttgcaaat aatacgaacc ttctgaatct
    38641 tctgaataga acggattttt attttgctgg tagccagcgg tcaatgagct acttctggta
    38701 ccgtcagctg ctaatttttc tttcagcaat tttttctgtg gattcgcttc taagagaatc
    38761 ccacctgctc catcgccaaa caacacagca gtcgtacgat cagaccaatc gattagctta
    38821 gatagtgttt caccaccaat caccagtccc ttagttgagc cgcagcggat cattttttca
    38881 gccatgctta acgcataaac aaatcctgag cacgcagcac tgatatcaaa agcgatagca
    38941 tgagttgctc ccaatttccc ttgtacttgt gctgctacag atggtgtcgt gtaatcgggc
    39001 gacattgttg ctactaaaat aaaatccaaa tctttagggt ttagattttt ctgttttatc
    39061 aatttttccg ccactaaaat gcataaatca gaagtctcct gatttgttac acatcttcgt
    39121 tcacgaatac ccgtccttga cgaaatccat tcatcagagg tatccatcat cttggaaaga
    39181 tcattattag taataatctt ttcaggaagg acgctggctg ttgcagtaat cattccaaat
    39241 tgttccatta aatatcctca cttatagtct tgcaagaagt cgtgcaagtt tttcagtgca
    39301 tccagcaagg catgctgttc ctccgtagcc attccgtcta agatgttctt gaccatttca
    39361 cgatgaaaat gctgatggac ccgaaatagt aactttcctt ttttcgttag tcccaacttt
    39421 acaacgcggc ggtcatcttc actgcggata cgttcgacat agcctttttt tactaacttg
    39481 ttaatagcag tggtcaaggt cccaacagta attgatagtt ctttggctac ttctgaagtt
    39541 gtttttttct tgtacatccc gattgcctcg atcgtgtgca tctctgtgat tgacagatct
    39601 ttgaattgag attttttcag ctcagactct tcgattgtca aaatatcatt gaagacacta
    39661 acaagataat catttactgt ttttaaatct ggttccatgt tccaccgtcc actaattagt
    39721 ttgattatca aatcttttga tgttcaaact atatcttaac ttttttcaaa atgcaacctt
    39781 aaaagatcat ttttttacta tatgagcttt ttttaagatt ttactcattt aaaacaactg
    39841 ataaatcagc atttataatt tcttttatta gaaaaatcat ctatctcaca tattttgatt
    39901 atcaaagtat tttctgtgaa cagaactata gaattcccta aaacttgtcc attatttcaa
    39961 aatttgttat agtcttttta ttcaaagcgt aaaaggagat cctatgttaa aagaaaaaat
    40021 gaaaactctc cccatgctgc cgggtgtcta tcttatgaaa gaccgagatg gtaaggtcat
    40081 ctatgttggt aaagcaaaac ggctcaaaaa tagagtaagc agctattttc atcagaataa
    40141 acagcattca aaaaaagtat tacgtatgat tcatcatata acagattttg atttcgtcgt
    40201 agtagacacg gaattagatg ctttattgct cgagtgtcaa ttgatccaac attaccgtcc
    40261 tttttataac cgacaaatga attatttcag taattataat tatgtacata ttaccaataa
    40321 gggatttgta ttaacagata ctcctacggc tcgaacatac gggccttttc gtctctataa
    40381 aaaaatgccg tctattttgc gtataatgga agaaacttat caaatgcctt ggttgtctga
    40441 aattagtttg ttagctttgc gagtccagct tcctgaccta caggaaatga cttttgaaca
    40501 gaaaaaaaag gaactccaag ggctgtttca aggccgaaat aaaaagctac tgacttactt
    40561 gaaaaaaaga caacaacatt tcatctatca gttgaatttc gaaaaagccg gtatgcttca
    40621 aaaagatatc gaattagtta cttattttat tagacggatc caggaacaaa agcagttttt
    40681 aagaacacca tcgctcacct tcagcatgcc attagcagca gatgagagtc agaaaaagca
    40741 ctacctcgtt tgttatggtc aacttgcaga aacaatgatt acctctggtg acgtttctcc
    40801 tgacttctat tatgaaaaga aagaagctca tttatctttg aaacgtcaat tgtcaaaaga
    40861 agaaatcgat cctgtacaaa tcttgatcag ttaccgcaaa aaattagaaa aagaacagtt
    40921 tgaaattgaa ctgttaaaca aaaaagaagc tgaaaagcaa ctaaattagt tgcttctcgg
    40981 cttcttttag gcatcttcaa cttcaaatgt caattatcct aaaatttatt ggattaagtc
    41041 atagatttcc atcgctacga tatcgatatt atcaaattcg tattgctgat gagtatctgt
    41101 ttcagttaat tcaaatgtat cggtcgcttt atcataagtt actgtacact tctctacgcc
    41161 ttctttttca aaacggcgaa cttgcagttc attatctgct gactcgacca tagcctccag
    41221 tcgttttatg attgcgacta attgtgaatg tgtcatcgga ggtcctcctt ttcttatttc
    41281 tgttatattg tatcaaaagc atagcctact tgcattgatt ttctctaaaa aaactcgttt
    41341 tttatgattt tttttctggt atttttcctt caggccacca aagtttgatc aaagcttggg
    41401 ttccgtcatt ctcaaaacct ttatctgcca actcttcata tagtttcaat gcttgggtag
    41461 ttgctggcaa tacaagatcc attttttctg cttcttctaa tgcaatcttt aaatctttga
    41521 taaaatgttt gacaaaaaaa ccaggtgtgt aatcttcttt taaaatacga ggagaataat
    41581 tgcttaaaga ccagttagct gcacttccac ctgaaagcgt ctcaatcact ttctttaaat
    41641 caagtccgtt tttttgtgca tagacaagca tttccgtcat tcctgtcatc gtacctgcaa
    41701 tcatgatctg attagccatt tttgtatgct gacctttccc agcagaacca tgcaatgtaa
    41761 atgtcgtacc aaattctttg aataaaggca gtacttgatc ataaacggct tcttctccac
    41821 cgaccatgat cgtcagtgta ccattctttg ctcctaagtc accaccggat accggtgcat
    41881 ctaacgcatc tgcgcctttt tcttttgctg tttttgcaat tttttctgcc aggcttggtg
    41941 tacttgtagt caaatcaact aaaatatgtc ctgagatatc tgcagaaaaa atccccgact
    42001 gtccatagta gatttcttct acatctttgg ggtaacctac cattgtaaag agaatattac
    42061 tttgttctgc taccgctttt ggtgtatctg cccagatagc tccttctgtt actaaaggat
    42121 cagcttttgc ttttgtccga ttatagacag ctacttcgtg tcctgcttct agtagatggc
    42181 gtgcgacagc acttcccatg acgcctgttc cgataaaacc aagtttcatt ttttcgacct
    42241 cctaaaaatc aaaaatcctt gcttctatgc tagtatacgc tagaaacaag gattttttca
    42301 taaaaaatgt ttgaatttta tcttttaaat gaatttaatt ttattttgat tcgttcaatc
    42361 gagtcttctt cttcttttct tcgcatctct tcttcatgat aaacaaccat gtcttgataa
    42421 aattcgatca tagcttcgcc gaaatgtgta gatgaaatat cataaagttt agcttctaaa
    42481 acttttggat ctttaggaac ttcactggaa atataatcaa taagtgctgg tgcaaagtct
    42541 tcgtcggttt caaaagtttt ccctaatgaa ggatgatcga acaatcggtt caaataagca
    42601 tttccttcca cgacacaagg tgttccggct gccatcgctt ctgtataagt aagcccttgt
    42661 gtttctgaag tagaagcact gacaaaataa tctgccgctt tataataaag tgctacatca
    42721 ttgttgtcca cttctcctgt gaactgcaca aattcttcta actgcagttc tgagatcaat
    42781 tcctctaact cttctacata aggtcctttt cctacgataa ctaatctggc gttaggataa
    42841 gcggagatga cctgaggaaa cccattaatc aaggcttgga tatttttctc ataagaaatc
    42901 ctgcttaaag aaagaatcat caaatggttt tcctgaagac ctagtgactc ccttaatttg
    42961 ctgatatcct gctgagtgat atcagaacgt ttgaattttt caatgtcgat accagtagga
    43021 atcacgcgaa gcggtgctat tacaccatag ctgcgtaatg tatcaataac acgttcactt
    43081 ggacaaacta cacctgtcgt atgatttgca aataatctag acaagtattt tacatgggat
    43141 tgcctgatca cttttccttt accgatataa tgaaggtagt cttcatacat cgtatggtaa
    43201 gtatggatca gaggtatttt caacttctta gctaccatct tcccaagtat ccctgctccg
    43261 aattctgtat gggtatgaat aagatctagt tctagttctt tggcaaccaa ataagcatac
    43321 cacatgcctc taacaacaac tcttcgatcc ttgaaagaaa taaatggtac acttggcatt
    43381 cgaatcacat ctttttccaa cttatccgca ttaggatcgg tcgttgtaaa aatatagact
    43441 tcatgcccat gtctttctaa ttcttgtttt aatgttttga tcgatgtagc aactccgctg
    43501 acttgcggaa agtaggtatc ggtaaaaaag ccgattttca tgtttttcgc ctccaaacta
    43561 gtccttgacc tgatttactt tttttaattt gctagagact ttttcattca gtacactttc
    43621 gtatacattt ttaagttctt caccgatttt attgatactt cgctcttttg ctgtttgata
    43681 accggcttgt gtcgtactcg gtagttcttg attggtaatc tgttggatca gtcggataaa
    43741 ttcttcgttt gtcttcccca tatagcagtt cttgctatct tctaaccagc cattatacac
    43801 tggaatatct cgaacaagta cgttttgttt gcttgctaaa gcttctaaaa caacgatccc
    43861 ttctgtctct tcataagaag ggaaaaagaa tagatctgca cctgaatacg caccttctat
    43921 gatctctcca cgaatgtaac caggaaacag gacattttca ggatgatctt cttttacaat
    43981 tttccggatg ttccgaggaa tcgagtacat gggaacatga ccaaaccaaa taaatgtgta
    44041 ttcgggcatc ttcttagcaa tttctacgaa atcaatgatt cctttacgtt cgaaaaacaa
    44101 accgacacaa atgattactt tttgatccgg agacagtttg aaatattctc tgaacttttg
    44161 ttctttcatt ggatcagggt gatatttctc taagtcgatc ccattggaaa tagcttgaat
    44221 cggcacacgg ataccatagc tttccaacaa ctgttttgaa taaggtgttg gcgtgatcag
    44281 atagtccgct ttttggtaca atcctaccag atattttttt acgataggag aaagctggtt
    44341 cgatccgata aaggaatttc ggaaatcttc ctctgtagaa tgcgcatgat agacaacttt
    44401 ttttccttct cttctggctt tattgaccat attatgacta ttgattccgt atgtattgat
    44461 gtgcaggatg tcataatctt ctttttcatc tagtgtataa gagataccga cttccgtcag
    44521 agcacgtttt tgatgatcaa gcgcacgccc gattccagat tttgcaagca tcttttcact
    44581 ttcaaaatat aataatacct tcaccttacg tcccccttca cttatatagt ataacatagc
    44641 ctacaaactg tcactcaaac ttaagcccta tttttgtcta ttcttactga caaaaacgtt
    44701 ttcttaaaaa agaacaaaaa aactggcgta gctcttttac aagccacacc agcttttgat
    44761 acgttgttta ttttacatat tcatgaacaa gttcaacgac ttcttccatc atatcgcatt
    44821 ctttcaatgc acgatcagca agttcagcca ttttcgctgt gtccaagcgt ttcattaaac
    44881 tacgcgtttt aagaatagat gtcgcactca ttgagaactc atctagaccc atacctacta
    44941 gcaatggaac agctgtttga tctccagcca tttctccaca catacctgcc cattttcctt
    45001 cagcgtgtgc tgcatcaatt acatttttga tcaaacgtaa aattgatggg ttatatggtt
    45061 ggtacaagta agaaacgcgt tcgttcatac ggtctgctgc cattgtgtat tgaatcaaat
    45121 cgttcgtacc tacagagaag aaatctactt cttttgcaaa cttgtctgct aagactgctg
    45181 ctgctggaat ctcgatcatg atgccgactt ggatcgtatc agatacttcc ttgccttcac
    45241 ttatcaattt ttgtttttct tcttcaaaga tcgcttttgc tgcgcggaat tctttgagtg
    45301 tagctaccat agggaacatg atacgtaaat taccatgaac agatgcacga agcaatgcac
    45361 gcatttgtgt acggaacatt tcgtcgcctt gttcagataa actgatacgc aatgcacgat
    45421 atcctaagaa tggattcatt tcatgaggta attgcagata tggtagttct ttatctccac
    45481 cgatgtccat tgtacgaaca actacaggtt ttccttccat accttctaat acagctttgt
    45541 aagcttcata ttggtcatct tctgttggga agtctggaga atccatatac aggaattcag
    45601 tacggtacag accaactgct tctccaccat tattatggac accaactaga tcttttggtg
    45661 ttccgatgtt tgcagctaat tcaaaatgct tgccgtcagc tgtgacagtt tctgcatttt
    45721 ttaatttttc ccattctgct ttttgtgctg cgtaatctgc acctgctttt tcaaattcag
    45781 ctttttgttc atctgtagga tcgatgatta catcgccttc gatcccgttg acagccaaaa
    45841 tatcgccttc tttgacttta gcagtaattt cttttgtccc aacgattgct ggaatttcta
    45901 aagaacgtgc catgatagca gaatgtgaag tacgtccacc aatgtctgtt acaaacgctt
    45961 ttacaaaatt gcgatctaat tgtgctgtat cacttggagt caagtcatgt gcaacaacga
    46021 ccacttcttc gttgatcatt gatggatttg gtaaagtaac gcctaataag tgcgctaaaa
    46081 tacgtttagc aacgtcacga atatctgcag cacgctcttg catataagca ttatcttcca
    46141 ttgcttcgaa cataccgata tacatatccg ttacttcttt tagcgctgat tcagcattta
    46201 cgctgttgtc tttaatattt tgcttgattt gacctaccat ttctggatct gaaagaacca
    46261 tcaaatgggc atcaaatact tgcgcttctg cttcacctaa gctttgcgct gctttttcac
    46321 gaatttgctg caattcttca gtagatttag ctaatgcagc atccaaacga gcttcttcag
    46381 ccgcagtatc ttctactgtt gccttactga atgttaaatc cggttgaact agcaggtaag
    46441 cttttgcaac ggctacaccg tcactagcgg caatcccttt tagcatttca accattattc
    46501 agataagcct tctttcttca ttgtttcaac gattgctgcc attgcatcag cttcgtcagc
    46561 accttctgca gtaatagtaa catcagaacc ttgacctaca cctaaagaca taacgcccat
    46621 gattgatttc aagtttactg atttaccttt atactctaag tttacatcag agttaaattt
    46681 gcttgctgtt tgtactaata gtgtagctgg acgagcgtgg atcccagttt ctgctactac
    46741 gtgaaattct ttcttttcca tattaatcgg tctcctttaa gtgagtgttg tttttatgtg
    46801 agggttttct tttttagtaa ttcagagaat cctaacaaat gccacccttt catctgtaaa
    46861 gattaacatt ttttttcgtt agatacaaca gttttcattc aatttcagat aaaagtattc
    46921 aaattattcc ggattctttt tcatcctttt cttcacattt tataacaaac gtgtttattt
    46981 cctttttctt gtcgctttct cttctcctga cgtttatcat tctttccttt gaccctcttt
    47041 gttttcatat gaaacgaatt acttgcttcc ctttttattt ttagtataat aagagtcaaa
    47101 ggtcaggaat ggtcaataga gatttcacct tttctacctc ataaatctct taaaaaacta
    47161 ggggttattc ttttacctca tgaaaaacga gatgtaaaat atggatgtac ccatttttgt
    47221 gaaaggacgt gaatgattca tgctttgtca aaactgcggc aagaatgagg caacgattca
    47281 cttgtatgcc actgtcaatg gtcagcggac acaactcgat tactgtcaga gctgttatca
    47341 aaaaatcaaa aatcaacaaa atggaggtct gactaatatg gcccaaaatg atccatttgg
    47401 atttggcagt ttggacgatc ttttccgttc catgtctcgc caaatgcaac agcaaggaaa
    47461 ttatgagcaa acaccaccaa cacaatttgg cggaaacaac aacttcaatg gtggtcagcc
    47521 acctcaaggc aatacagaag gtttattggg agaatacgga atcaatatca cagaacaagc
    47581 acgacaaggt gatatcgatc ctgtgatcgg acgcgatgac gaaatcaaac gagtcattga
    47641 aattttaaat cgccgaacca agaacaatcc tgtcttgatt ggagaaccag gtgttggtaa
    47701 aacagcagtt gttgaaggtt tagcacaaaa aatcgtagat ggtgatgtac cgcaaaaatt
    47761 aatggacaaa gaagtcattc gtttagatgt cgtatcttta gtccaaggta ctggtatacg
    47821 aggacaattt gaagaacgta tgcaaaaatt gattgaagag atccgtcaag cagaaaatgt
    47881 gatcctattc atagatgaag ttcacgaaat cgtcggtgct ggttctgctg gtgatggaaa
    47941 tatggatgct ggtaatattt taaaaccagc tttagctcga ggagaattgc aaatggttgg
    48001 tgctactacc ttaaatgagt accgcatcat cgaaaaagac gctgctttag aacgtcgtat
    48061 gcaacctgta cgggtagatg aaccaactgt cgaagaaacg atcagtattt tgaaaggttt
    48121 gcaaaaacgt tatgaagact atcatcatgt aaaatatact gatgaggcca tcgaagcagc
    48181 cgcaacttta tccaatcgtt acgtccaaga tcgcttcttg ccggataaag ccatcgattt
    48241 gctagatgaa acaggttcta agaaaaactt gacgatccaa atcgttgatc ctaaaacaat
    48301 cgagaaaaaa ctgcaagaag cagaagaaca aaaagtttta gcttctcgag aagaagattt
    48361 cgaaaaagca gcttattatc gcgatcagat caataaactt caaaaaatga aagaacgtca
    48421 gctcactgaa gaggaaacac ctgtgattac cgaaaaagat atggaaaaaa tcgttgaaca
    48481 acggacaggt attccagtgg gtgaattaaa agaaaaagaa cagacacaac tgaaaaactt
    48541 agcagatgac ttgaaagctc atgtcatcgg acaagataac gctgtagatc gagtagcgaa
    48601 agcgattcgc cgaaaccgtg ttggattaaa caaacagaat cgtccaatcg gctccttctt
    48661 atttgttgga ccaactggtg taggtaaaac agagcttgcg aaacaacttg cctacgaact
    48721 attcggttca caagattcaa tgattcgttt cgatatgtct gaatatatgg aaaaacatag
    48781 tgtctctaaa ttgattggtt ctcctccagg ttatgtcgga tacgaagaag ctggtcaatt
    48841 aacagaaaaa gtacggagaa atccttacag tcttgtcttg ttagatgaag tagaaaaagc
    48901 tcatccagat gttttgcata tgttcttgca aatcttagat gatggtcgat tgacagatgc
    48961 acaaggaaga acggtcagct ttaaagatac gatcatcatt atgacaagta atgccggaac
    49021 tggtaaagta gaagcaaacg ttggttttgg tgcggcacgc gaaggtgtta ctcgttctgt
    49081 attgaaccaa ttaaacaact actttacacc agaatttctg aaccgttttg acgggatcat
    49141 cgaattcagt gcattatcaa aagaaaactt gatgacaatc gtcacactaa tgctagacga
    49201 tgtcaaccaa atgctggcag cacagcaact tcacatcgaa gttcctacga atgtcaaaga
    49261 aaaattagtt gacttaggct atgatccatc tatgggtgct cgtcctcttc gacgcacgat
    49321 ccaagaacaa atcgaagatg gtatcgctga attttatctt gatcaccctt ctatccatga
    49381 gttgaaagca aaactggata aagatggaaa aatcatcgtt acttctaaac cagaacgttt
    49441 ggctaaagaa tcagccgaag aaacggctga ataaatccag ttttatagcg tgagacggta
    49501 tttctttgag aaatgccgtc tcattctttt ttcagaaaag tcaaaatact gttcatttta
    49561 taaaaaagaa attataataa gagaaacaag caattaaaaa tacataaaag aggtgagcgc
    49621 catgaaattg gtaaatgtaa caaacagcca ttcccgtctt gtattaaacc agcttgaaaa
    49681 cactgatgcc catctagtca aggtctacac agccggaaat accactattg tctacactga
    49741 agcccctgaa cataatgaaa ttctgctgat caatgacaaa cgtaaaatcc agccgaaaga
    49801 aatcgaagat gctaaaaatt atttcttaga taaaatcaaa gacgccgttt accacgagga
    49861 tgaaatcaaa gtaatcgaat tggcaggcct tgttgaaatc tcgattccaa aacatgaatt
    49921 aggcgctaca ttagccgtgt aaacaaattt tgaacataat aattaaagag cgtgggacaa
    49981 aa
//
LOCUS       DAFBQH010000011        46150 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.011, whole genome shotgun sequence.
ACCESSION   DAFBQH010000011 DAFBQH010000000
VERSION     DAFBQH010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.011"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(392..1459)
                     /locus_tag="LK694_000732"
     CDS             complement(392..1459)
                     /locus_tag="LK694_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK5051623.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(1479..1958)
                     /locus_tag="LK694_000733"
     CDS             complement(1479..1958)
                     /locus_tag="LK694_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HBK5051624.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(1975..2436)
                     /locus_tag="LK694_000734"
     CDS             complement(1975..2436)
                     /locus_tag="LK694_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK5051625.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(2450..3700)
                     /locus_tag="LK694_000735"
     CDS             complement(2450..3700)
                     /locus_tag="LK694_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBK5051626.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(4078..5529)
                     /locus_tag="LK694_000736"
     CDS             complement(4078..5529)
                     /locus_tag="LK694_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBK5051627.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(5850..6614)
                     /locus_tag="LK694_000737"
     CDS             complement(5850..6614)
                     /locus_tag="LK694_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBK5051628.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(6642..7340)
                     /locus_tag="LK694_000738"
     CDS             complement(6642..7340)
                     /locus_tag="LK694_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBK5051629.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(7352..8131)
                     /locus_tag="LK694_000739"
     CDS             complement(7352..8131)
                     /locus_tag="LK694_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBK5051630.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(8147..9091)
                     /locus_tag="LK694_000740"
     CDS             complement(8147..9091)
                     /locus_tag="LK694_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBK5051631.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(9137..9916)
                     /locus_tag="LK694_000741"
     CDS             complement(9137..9916)
                     /locus_tag="LK694_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051632.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(10296..12371)
                     /locus_tag="LK694_000742"
     CDS             complement(10296..12371)
                     /locus_tag="LK694_000742"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBK5051633.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(12373..13290)
                     /gene="glyQ"
                     /locus_tag="LK694_000743"
     CDS             complement(12373..13290)
                     /gene="glyQ"
                     /locus_tag="LK694_000743"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBK5051634.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(13676..14494)
                     /gene="recO"
                     /locus_tag="LK694_000744"
     CDS             complement(13676..14494)
                     /gene="recO"
                     /locus_tag="LK694_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBK5051635.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(14637..15536)
                     /gene="era"
                     /locus_tag="LK694_000745"
     CDS             complement(14637..15536)
                     /gene="era"
                     /locus_tag="LK694_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBK5051636.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(15551..15946)
                     /locus_tag="LK694_000746"
     CDS             complement(15551..15946)
                     /locus_tag="LK694_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBK5051637.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(15930..16406)
                     /gene="ybeY"
                     /locus_tag="LK694_000747"
     CDS             complement(15930..16406)
                     /gene="ybeY"
                     /locus_tag="LK694_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBK5051638.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(16435..18627)
                     /locus_tag="LK694_000748"
     CDS             complement(16435..18627)
                     /locus_tag="LK694_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBK5051639.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(18651..19622)
                     /locus_tag="LK694_000749"
     CDS             complement(18651..19622)
                     /locus_tag="LK694_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBK5051640.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            20327..21706
                     /gene="brnQ"
                     /locus_tag="LK694_000750"
     CDS             20327..21706
                     /gene="brnQ"
                     /locus_tag="LK694_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBK5051641.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(21862..22308)
                     /locus_tag="LK694_000751"
     CDS             complement(21862..22308)
                     /locus_tag="LK694_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBK5051642.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(22335..22511)
                     /locus_tag="LK694_000752"
     CDS             complement(22335..22511)
                     /locus_tag="LK694_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBK5051643.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(22673..23101)
                     /locus_tag="LK694_000753"
     CDS             complement(22673..23101)
                     /locus_tag="LK694_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBK5051644.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23193..24053
                     /locus_tag="LK694_000754"
     CDS             23193..24053
                     /locus_tag="LK694_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBK5051645.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(24147..24497)
                     /locus_tag="LK694_000755"
     CDS             complement(24147..24497)
                     /locus_tag="LK694_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBK5051646.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(24513..26240)
                     /locus_tag="LK694_000756"
     CDS             complement(24513..26240)
                     /locus_tag="LK694_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBK5051647.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(26453..27379)
                     /gene="trxB"
                     /locus_tag="LK694_000757"
     CDS             complement(26453..27379)
                     /gene="trxB"
                     /locus_tag="LK694_000757"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBK5051648.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(27524..28960)
                     /locus_tag="LK694_000758"
     CDS             complement(27524..28960)
                     /locus_tag="LK694_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBK5051649.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(28957..30360)
                     /locus_tag="LK694_000759"
     CDS             complement(28957..30360)
                     /locus_tag="LK694_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBK5051650.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            30590..31492
                     /locus_tag="LK694_000760"
     CDS             30590..31492
                     /locus_tag="LK694_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK5051651.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(31533..33419)
                     /locus_tag="LK694_000761"
     CDS             complement(31533..33419)
                     /locus_tag="LK694_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK5051652.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(33516..34368)
                     /locus_tag="LK694_000762"
                     /pseudo
     CDS             complement(33516..34368)
                     /locus_tag="LK694_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            complement(34703..36487)
                     /locus_tag="LK694_000763"
     CDS             complement(34703..36487)
                     /locus_tag="LK694_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5051653.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(36487..38238)
                     /locus_tag="LK694_000764"
     CDS             complement(36487..38238)
                     /locus_tag="LK694_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5051654.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(38380..38604)
                     /locus_tag="LK694_000765"
     CDS             complement(38380..38604)
                     /locus_tag="LK694_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBK5051655.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            38855..40441
                     /locus_tag="LK694_000766"
     CDS             38855..40441
                     /locus_tag="LK694_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBK5051656.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(40507..42282)
                     /locus_tag="LK694_000767"
     CDS             complement(40507..42282)
                     /locus_tag="LK694_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBK5051657.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(42493..43395)
                     /locus_tag="LK694_000768"
     CDS             complement(42493..43395)
                     /locus_tag="LK694_000768"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBK5051658.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            43674..46019
                     /locus_tag="LK694_000769"
     CDS             43674..46019
                     /locus_tag="LK694_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBK5051659.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
BASE COUNT        13200 a         9779 c         8016 g        15155 t
ORIGIN      
        1 tatgctttcc cttggaataa tgaaaatata cttttgtatt acacaatatt gcaattttat
       61 ttcttaatat gattatttca ctcattcatt actgccacat atacacaact tataaccacc
      121 ccctgcaaag tttagggtaa gttatcagat aatatcttcc tcttcatcat ccagaataca
      181 aaatctattt atttatagaa atatcattaa tgtttttata taatgtaccg tgcttgtact
      241 cttgctctat tacaagcact aaaacactac tattcatttg aattcttaca cggataaaaa
      301 tttacatcgt ctatttcaat attttttctc tctattttat tcacttaata agttcattac
      361 ataatcgaaa cattttttat tttccttgtc atcactcctt gctccctttt tgatacagat
      421 ttaaaattct atcaaagaaa tttccatcgt taaatttcgt tattgccaaa tccttcgaac
      481 aacgtgaaaa ttttacgtat ttatctttat cattgatttt tagtattgca gatgtcaacg
      541 ctgatacgtc accaggttga aacaaatatc cattttctcc atcacgtact gctgaagaaa
      601 tatctcctac atctgtagca attacaggca ttccataaga aatcgcctct agaatagata
      661 taggcaatcc ctcgttatag gacggtaaaa tcatcacatt tgatcttcta tagcactcct
      721 cctttttttc tcccacaacc caacctgtaa attcaaaata cgattgcaaa ttatattctt
      781 ctatcaattc tttgagcgat atttcttcat cacctgctcc agcaatctca aaaataaact
      841 ttttttctgg atattgttct gcaatgtttt taactgcttc taccaaatcg tgtacacctt
      901 ttcgctttat caaaactccc ataaacagaa atcggcacat ttttgaattc cactttatgg
      961 tatattctgg aactacaata gaattttcta caacaacagt tttacaattt ggctcaattt
     1021 ctttaattat cttcttccat ttttctccaa gtacaatcgt ggaatcagac tctcttaaca
     1081 gcaagcgaat ctgattttgt ttttttacag aaacactatc ataccatttt ttaaattccg
     1141 atccatgaag atggatgatt acaggaatat ttaataatct aaaccactta ttcagcacat
     1201 atttcctact aaaactccct ctgtacgaca tgtgcatatg aataatatta ggtttactca
     1261 ataaagagat acctataatt ttagcaaatg catacataaa atataaagat ttaaatattg
     1321 catttccacc cttataactc ggtataaaag tcatctcgat attctttttt tcccaattaa
     1381 aattgagcat ttgtgtaatt atacttgtaa ttccaccttt taccgagctg tcattaccaa
     1441 tcataagtac tctttgcata acgctttact ccttatattt atattttttc tattaatacc
     1501 tctatcgaat taataatcgt ttccgtgtta gatacatact tgttaaacat ccgaactctt
     1561 gtttctttaa cggcatcttc taaattttct aattcataac aaggatagat aatattcatc
     1621 tcatcaaatt gtctaattaa ctgcagttgg tgatcatcga catgctcacc atacttagcc
     1681 aatcgaggaa cagcaatgac ccttttacct ttttttacgg ccccaattat agcacctgta
     1741 cctccatgag taatcaccat ttctgctttt tgcatagttt cagtaaactc atccctattt
     1801 agaaatgctt tataggaata atttagtggt ttataatcac tataaccact ctgtgcaaat
     1861 gtactttcga ttattactcc tttttcaatt aaatcatcaa tagcttttaa caatctatca
     1921 aattgaaatt tttgtgaacc taatgttata aatatcattt ttttctccta ttatttaata
     1981 tatcccaccc aaataaatag cattaggata aaattgtagc atagtttccc actgaacata
     2041 aaattgatct gcaaaatgat acatcaattt tcctgtctga gtagggctgg ttacttttgc
     2101 aaacgattca atataaatca gtttttttcc catcattttt gttattaagc agatagggat
     2161 catagctaaa acacctgtac aaatcacgag atccggtttt tcctttatat atataataat
     2221 tgatttgaat aaattaataa gtatagtaca caaaaaaaac ttttcttttc tatttacttg
     2281 tctcatgtag tacgtttttt cgccttttga ctcagctgaa tattctgtct gttctgttat
     2341 aacaaaactc tcatacttct ccatcagtgg ttttaacatc attaactgtt cataatgccc
     2401 cccagaggaa gccgcaaaac atatcttttt gttcatatta acctctctat cagtcacgct
     2461 gaaaaatatc tctagtgtaa actttttctt taatatcatc caagcaggta tcatatcgat
     2521 tggcaataat agaatcacac atcgccttaa actgctctaa atcgttgata accttacttc
     2581 cgaaaaaagt ctctccatct ttcagtgcag gctcataaat caccacagtc gcacctttag
     2641 cttttacgcg cttcataact ccctggattg aactctgacg aaaattatca ctgttgctct
     2701 tcatggtcag ccggtatata ccgattaaaa ctggattttc cctgttgaca tcatactgat
     2761 tatattcagc gtagttgtaa tagcccgcca tctgaagaac acgatctgcg atgaaatcct
     2821 tacgggtacg attagattca acgatagcct caatcaaatt tccaggaaca tctgcatagt
     2881 ttgccaacaa ctgcttggta tcttttggca agcaataacc tccatatcca aaggatggat
     2941 tgttgtagtg agttccaata cgaggatcga gacaaacacc attgattatc tgctgagtat
     3001 tcagcccctt catttcagca taagtgtcta attcattgaa atagctgact cgcagtgcta
     3061 aataggtatt tgcaaacagc tttactgctt cggcttcagt gaatcccata aaaagagtat
     3121 ccacgttctc cttaattgca cttttctgca gaagtgttgc aaaaacatga gcagcattta
     3181 acaagcgctc atcagtcata tcagtaccaa caataatacg agatggatac agattatcat
     3241 ataaagcctt gctctcgcgc agaaactctg gactaaaaat aatgttctta ctccccgttg
     3301 tctcacggac tgattcagtg tagccaacag gaattgtaga cttgatgacc atgatggtat
     3361 caggattcac gtccatcaca acctcaataa ctgactcaac agcagaagta tcgaaatgct
     3421 gagtacgact gtcatagtta gtcggtgtag caataacaac gaaatctgcg gatttataag
     3481 cgctccttgc atccaaagta gcagttaagt caagttcttt ttctgtaaga tacttttcaa
     3541 tatattcatc ctgaattgga gacttccgtt tattaatcag ttcaatcttt tcagggataa
     3601 tgtctaccgc ggttacctgg tgatgctgcg ccaatagtgt ggcgatactc aaaccaacat
     3661 aaccggtgcc ggctactgcg atgttgtact ttttcttcac aatatctcct ctttcctctc
     3721 ttaagtatgg agaatgttac tcctacggct attcatcatg tcaaaataac acagcttaga
     3781 atgttgataa aataattttc cagaatgtga taaaatctct ttcatttata aaccacacat
     3841 ctaattttat ctattctcca caagacatgt agagtattct ttaataacaa gatacgacct
     3901 tactaaatac ttcaaaaaat tatcatattt aattcataaa tataaatctt cattttaaat
     3961 gtatgcccaa aaatttatct gtaatgcgtc atcaaaatct agattccaaa tctttatcaa
     4021 tttttatcca ctcattctac cactttagta atattttcta tttataacac tttcgtccta
     4081 cattgatccc cccctctgaa gtacagtttt tactgtctta aataaaattt taaaatctaa
     4141 ccccatactc caatttttta tatattccgt gtcaagtttt gctacttctt caaaatcagt
     4201 aatatcactt ctaccactta cctgccacat tccagtaatt cctggcttga tagcaattct
     4261 tgcacgatga tgataatcat attcttcata ctctgattta aggcatggtc gaaatcctat
     4321 aaggctcatc tttcctgtta gaacgttgaa aaattgaggg aattcatcta aagacgtgga
     4381 tctaataaat tgaccgattc ctgtcttttt tgttccatct ggaagtattt tatttccgat
     4441 aattcttgga tcaaactcca tcttaaacat aagattattg ctcatcttat tttctttcat
     4501 taattctact tttctttctt ctgcatccat atacatactt ctaaacttat acatcttgaa
     4561 cggtttacca ttctgaccta tacgttcttg tgcaaagaaa attgggcctg gtgaattaat
     4621 ataaataagt ggagcaataa agataaaaat aattcccgtt gcaatacatc caacaatacc
     4681 tgcaagaata tcaattgttc gttttataaa tgcctgttga gttgtcataa cattgatact
     4741 tgttgtaagt actgtatatt ctcctatacg accaatagat tgttttccca aagcactatc
     4801 tgatactttt gcaagattca aatgtacagt aaggcccatt tcagagcatc gttcaataaa
     4861 ttctttcata cacgaagtac ttttattcaa aataataaag acttcatcta cccatccctg
     4921 acataagtaa tccgctgcat ttttggaatc agaaacaact gtcactccct caacatcttc
     4981 accaatcatg cttttatcta tgataatgac accatttatt ttatacattt tatagttatg
     5041 ctctttcaca ttacgaataa ctacttcagc aatactacta gatgtaacta taataagtga
     5101 tttgttcccc tcatttttca ttttatgttt caagtaatgt ttccataaga ggcgagttat
     5161 atatgtaatt acaacgtata aacctcccat caagaaaaga acgactctag aataatcatt
     5221 tccaccctga acactaatga gatacaaact tcctaacagc accaacataa tcgcttgctt
     5281 tacagaagaa gcaaattcta tatagtaccc tctttttaat acacctctat atgcttcaaa
     5341 catgaaaata actaaaagat ctgccagttc aatgaagact gccatgtttt gatacattgg
     5401 aatcacataa ggattccaat ctacctggcg taagacatac gatagataaa aagcaagctg
     5461 gatacacaat aaatctaaaa taataaaatc aatatgttta acccatcccc ttgaactttt
     5521 cttgtacatt cctttttact cccctctttt taataaaata aattgtatta cttgttttct
     5581 ctcctaaata tctttctgaa ggattattga ctcgacaaat tggcttgtgg atattcttat
     5641 atatagaaca tccgtaagcc aacttaaaac tccttaacaa aattttttta ctcatattgt
     5701 agatcaaaac aagtatccaa gaaatcatat tttatttctt ttttctgctt actcaacaac
     5761 agctaaaaga aaaatcatca taaacaaatt caactaagcc aacgatcccc ttgtgaaatc
     5821 aagttttttc ttgatttacg aataaagagc taaaataaac caaacttctt cttttttacc
     5881 tcttcataga ctggataact gatttcatcc ccgttgatca gatcctttgc gacttgtttc
     5941 atctgtacta ctttttcctt accaaagtcc tttgcaatct gctcatagca ctcttttaag
     6001 taaaaggtgc gcttagagac cccatgggca tcagaagcag ccatctgcac gagattatgt
     6061 ttgaccatcg ttttggctgt tttctggatg tctttgccaa ataccccaac ataacttggc
     6121 gcagtgagtt gggccaaaca tcccatatct aggaacggaa gcaaatgatt tgggtcttcg
     6181 cgaaaatgca cgttgcgttc tggatggacg attactggtg tctttcctag ttctaacaac
     6241 tcaaaaaaaa gccgttctgc atataacggc acttccagtg tcggaaattc gatcagaaga
     6301 tatgtatcat ctaaatctgt aaataatatt tcatctcggt ggatgtcttc gatgagcgtt
     6361 ccggtaatac gtacttcttg tccttccaat aacgtcaaag gcaactgtct tttctctaat
     6421 tcggcttgta acgaagcgac tagagaaatg acttgtgatt tttcattgct gtattttcca
     6481 ttattgtggt gtggtgtgca taaaatatgc gtgatgcctt gctgaatcgc tttttcagcc
     6541 attgcaatac ttgcttctaa gttttctgcc ccgtcatcga ttcctggtag gatatggcaa
     6601 tgtagatcga tcatttctct tgtctcctct cttcttattc cttaatttcc gtagtaataa
     6661 ccagcatctt tgctatgttc tgctccgttg tacacaacac ctaagactcg tgcttgtacc
     6721 atctccagca gttcttttgc tttagtcaag gattctttac gtgacatatt ttcacgtacg
     6781 accaataaag tgccatccgt ttttaaagac atgatctgtg catctgttac ggctactact
     6841 ggtggcatat cgaatatcac cacgtcaaat aactgtcttg cttcggatag gatctgatcc
     6901 attcttggcg agcttaacaa ttctgatggg ttgggtggct taggtccact aggcaaaatc
     6961 gacaagttct cgactggcgt tcgttggatc acatctgcta cacttcctga tgaactaagt
     7021 gctgtactta atccactggc attgttcagc tggaatgtct tatagacgac gggtttacgt
     7081 aaatctgcat cgactagtag aactcgttga cctgatttag caaaaacgac tgcaatgttt
     7141 gctgcagtgg tggatttccc ttctccagga cctgatgaag tcacgacaat cgttttgatt
     7201 tgctgaccag ctgcggaggc aaactggata ttcgtccgga tcgtacgata ttgttcggcg
     7261 attggtgaag atgggtttgt taatgtgatc aaactgactg catgagtttg tgctgctttt
     7321 tgtttctgtg ttcgtgccat ttttcttcct cctagactct tgagcggctt ctgcgtgatg
     7381 gggctgtttt atcgtttgta gcttttggtc gtgcaggagc tgttcctttt ttacggatac
     7441 cagcgttcaa ttctttcgct gacatttctg gaactgttcc taagattggg aatcctaatg
     7501 tatctgtcac atatttttca tctttcactg tccgatcgag taattccaat aagaatgcca
     7561 gacctacacc taccatcatt cctaacatta agccagtcat taaattcaat ttattatttg
     7621 gcgaaacagg tgtcgtattg gcaactgcac ctgaaatgat cgagatttta tcaacattca
     7681 tcacatcttt tgcgttctct tggaaaactt gtgaagttgt gttagcgatc tgttgagcag
     7741 ccactgcatt cgtacttatt gcctgaatcg agaacatttg tgaattttct gcctgattaa
     7801 ctgagatggc atctttgatt tgttcagcag tcattttcac attgtcttct gtttctaaac
     7861 gatctttgac ttcattgagc actaaatcac tgacgatcat atctttatac gtattgatca
     7921 tttgcaggtt cgtattgaca tcattgacat ttgcagtgtc tgattgtggc agtgtcacga
     7981 tcaattgtgc ttgtgaactg tattttggtg tcaagatgaa aaatgttacg attcctgaga
     8041 tggccaagcc agcaaacatt gagatgatga ttattaccaa gtgtttgcgt aatgtttcaa
     8101 aaatttcttt caagctgatt gtctcttcca ttttttctcc actttcctat ttattctcta
     8161 gattcatttg atcttttaat tcttttttca cacgatcaag ttcttgatca ctgacttttt
     8221 gataggagat attatcttgc atgaagcctt ctccttgcag ctgatcttgc ttaatcgtac
     8281 caaatgcctc ccgataatct aaagcaattt ttttcatttc atcaaagttc atgtctgttt
     8341 tcatattcga ctctagagca ttcaaaacgg attgatagtt gctgacaccg tttagactca
     8401 atactttttt cgctacgcct tctacgatct ttctttggcg cccttgtcta ccgtaatcac
     8461 catttgggtc ttcataacgc atacgagaat aagataatgc ctgatccccg ttcaatgtaa
     8521 ttttaccgat tggaaagtca tagccatctt gtgagaaagt gaggtcatta ttgacttcga
     8581 taccaccaac tgcatcaacg agttccttta gtcctttcat attgatagag acatagtgat
     8641 cgattgggat gtctacatat ttttgtacgg tatccatcgc catggcagct ccgccgaatg
     8701 cgtaagcgtg gttgatcttg tcttttgtgt cacgacctac gatgtcgaca tacgtatctc
     8761 gaggaatgct gacgatcgtt gtctgtttgt cttcaggact gactgtggca accatcatcg
     8821 tatctgatct tccttgatcg acccgaccta aatctccagt atcaatcccc atcaatagaa
     8881 cagagaaaga ttcttgtttg tccagatcaa ctggtttcgt tcgttctcct tgggttcgtt
     8941 ccactgattc gtaagttttc tcaatagaat gtgagagatc cagatataat tttgctccta
     9001 ctcctacaat tgctaatact aatacgataa gaactgctaa aataatcaaa aatatttttt
     9061 tccctcttga ttttcttctt cgtcttgcca tagtttcctc cgtttgctaa ccttgcttga
     9121 atcaacgttt taactgtcaa tagcttatat tgctatagcc catttgctga gcaacagttt
     9181 gtgcttttgt tgcgtatgcg gctcttattt tttcattccg gatctgctct atttgactga
     9241 gcgccgtcaa atattgatcg taagtagcgg tagctgttac ttgaccgtct ttcagcatcg
     9301 tatcgaatag ctgatctagt tgcgtgactt gtgcaatttg ggtatcagcg aatcctagat
     9361 attgcataac gactgttttc caagcactat ccttaaaaaa gctcatattt tcacgtacat
     9421 gggcgacatc tgcacttgcc aacttttctt tgatgatgac atcatccaca gcttgctgcc
     9481 agtttgatac gtttttagta aataatttat tgacagccgt ctggatcttc agttttgatt
     9541 ctgcatcctc taactgcgtt aagactgctt tcttttcttc ttggatcgca gcagcttctt
     9601 taggtaattc tgattccttg atgtcataat catctgccga aagtttgata gaagatagtt
     9661 tatgtgacaa atttgaaact gtttcttctg ttatttcagg atttaggaaa attttttctt
     9721 ttgtactgta cagcttatct acttcttttg ataaagaggt cacggtgtct ttttgattgg
     9781 tgatggcaga ttctgcgtaa ttgatccgtt gatgatattg ccgctgatta ttgagatggt
     9841 atccaaacaa gcaaatcaca atagcgcctg tgcctaccgc cacagtagcc aataacttga
     9901 cgaacggctt ttccatagat tcactcctaa atattgatga tagataagta aagatgtgat
     9961 tcattcacat cagaatgagc gctttttatt ttaaaagcat aattaaacta atatattcat
    10021 agaaatattt ataaacaggt tttccctata taatcatacc acatacagtt tttttgacta
    10081 gggaaattcc taatttaaaa gtaaataaaa cagtaacaag attataatta atcgttttca
    10141 tttttatttc ctcacttttc atgagcgttt tgaaaattcg aacaaagaat aaaataacaa
    10201 aaattattaa aatagaatta ataacagcta tttataacaa aaaaaacaga gacacagcac
    10261 gtttcgtgca tgctctctgt ttttcaaaca aaagactatt ttgtgatgat ttgatccaag
    10321 cttgctaatg acaaagccat ggcagctatt tgacttagtt gtgttagtcg gttcttacgt
    10381 actgctggtt catcagccat gaccatcgtt tgatcaaaat aattttcgat cagtggacga
    10441 agagcgacaa gctggtcgta gttttcgtgg atcgtttggt cagcaaaact tgctttcatt
    10501 gcttctactg ctttgtgtaa ttctttctct gcgtcgtttt cgaacagctc agggtctact
    10561 tctgattgtt tttcaagctg cgcttttttc gctaagttca cgacacgtgt caaagcttcc
    10621 attgaaggtt tgaagtcttc atctgcaaga tgttgtttga acaattgtgc tgctgcaaat
    10681 acttttgtca gatctttttg ttccgcattc aaaacagcat cgatgacatc atgacgaata
    10741 ttcttcgttg tcaataattg gcgcagacgg cctttgataa agtcgatcac ttgttgttgc
    10801 ccttctgata aaccaatgcc gaaacgatcc gtatctttac tgataacatc gctaatcgtt
    10861 tcttgcagaa cagacaaagg aaaggcccat tttttcgatt cgatgatccg aacgacaccg
    10921 tatgcttggc gacgtaaggc ataaggatcg tttgacccgc ttggaatcat cccaactgca
    10981 aagaaagaaa agatgctatc taatttatct gcaatcgcta aaacagaacc tactgtagac
    11041 tgaggtagct ctccttcact ggatataggc atgtaatgct cacgaatcgc tgtcgctaca
    11101 gcaggtttct cgcctttcag aagagcatat ttttcgccca tgatcccttg tagttcagga
    11161 aattccccta ccatgttggt tactagatca aatttataga tttcagcagc tcgttgcaaa
    11221 tccatcaatt cttcctcaga tagtcctact tgttcgccaa tgatacgggc aatgacttgt
    11281 acccgttgca ttttctcata gatactaccg attttttcat ggaaagtaac agtttttagt
    11341 ttttcaacgt attggtcgat tgaagctttc ttgtcttcat tataaaagaa ttccgcatct
    11401 tctaaacgag ccgtcaatac tttttgattg cctcgaacca catcttccaa ttttacccga
    11461 tcaccattac gagcagaaat aaaatgagga agcagcatac cttgatcatt ccgtacctcg
    11521 aaataacgtt gatgttcttt catagaagtg actagtactt cttcaggaac agaaagatat
    11581 ttttcttcga agtcgccaga aaagacagtt ggatattcga ctaaattgtt gacttcttct
    11641 agtaaatctt cgtctagatc aataacccag tgattttttt ctgctaattc gtttgcttgt
    11701 gtaacgatca tttcttgtct tttttgcgga gacgcaatga cgaattgttc attcaatttt
    11761 tcttcatatt cttttgcatg cgcaaatgtc acatcttcgc ctaagaaacg atgacctcga
    11821 gaggctctgc ctgttttgac atccaagatt tcgaatggga ttacttcgtc atctaacaat
    11881 gccacgatcc agtgaatagg gcggatatat tcaaaatcgt attctgccca atgcatcgtt
    11941 acagggaaag tcaaactttt gatcacccct gctaagcctg tcaatacttc ttttgcttcc
    12001 ttaccatgag tgtatttcgt tacatagaca tattccacac cttttaattc tctaaaagtg
    12061 atagcgtctg tcgtcagacc ttgtccgcgg acaaatcctt ccgctgcttt cgtccagttt
    12121 ccatctgcgt ccaaagcaat ttttttggct ggtcctttga cttcttcttc actgtccgct
    12181 tgtcgttctg gaatatttgt tactttgatt gctaaacggc gcggtgtgga aaatgtttcg
    12241 atcgtatcat aagtcaattg gttttcatct aaaaattttg ctgttttttc ttctaattgt
    12301 ttcatgcttg gtgtgacaac atgggcaggc atttcttcca gcccgatttc aagtaataag
    12361 tcttttgcca tcttattcag cctccttcac ggtttctgga tttgctgtgt ttttattcaa
    12421 caaagggaat cctaattttt cgcgttctgc cacaaagatt ttggctacag cacgagccat
    12481 gttgcggatg cgagaaagat aaccggcacg ctctgttacg gatactgcac cgcgggcgtc
    12541 taataagttg aatgtatggc tacatttcaa gatataatca tacgccggat gaacaagatt
    12601 ttcatcgata cagcgtttag cttctttttc aaaacgatca aagttgctta gtagcaagtc
    12661 ttggtcgctt acttcaaaag aatatttgga atgttcatat tctggttgtt tgaagatctc
    12721 gccatatttc acgccatcag cccattctaa atcatagaca ctttcaactt cttggatata
    12781 agaagcaaga cgttctaatc cataagtgat ctcagaagtt actggtttac aaggcagtcc
    12841 accaacttgc tggaagtacg tgaattgtgt gatttccatt ccgtcaagcc atacttccca
    12901 acccacaccg gcacagccca tggatgggtt ctcccagttg tcttcaacaa aacggatgtc
    12961 gtgttctaat ggatcgattc ctaaaaggcg caagctttct aaataaagct cttgaatatt
    13021 ctcaggtgaa ggtttcatca ccacttggaa ttgatgatgc tgatacaaac ggtttgggtt
    13081 ttcaccataa cgtccatcag ctggtcggcg agatggctct acgtaagcag cattccatgg
    13141 ttctggtccg attgcccgta aaaaggtata tgggctcatc gtacctgctc ctttttctgt
    13201 gtcataagct tgcatcagca tgcagccgtt tgatgaccaa aatttttgta atgttaaaat
    13261 catatcttgg acggtcaatt ttttactcat ctgttttgcc cctttcaatt ttaaagcgtt
    13321 gctgtcaaat aagaatacag gaaagaaaac gacaaaaaat ccctatgtct gattacagac
    13381 atagggacga ataattcgcg gttccaccct acttccgaat aaagattcgg cagcttcatt
    13441 tatcgtactc gcaagtgcct cttcaaaaat cgtttgggtc cggctcccac tctcccgaac
    13501 tcgctaaacc acgtgttttt tactgatctt ggtcaatgta cgtattcatt ttgcagtttt
    13561 cttgtttcta taatagatcc tatcactgaa cgatctattt aatctatggt taataataga
    13621 cggaactccc tgtgtttgtc aaatggtttt gattttttgt tgtatgtttt atttactatt
    13681 cttctttttc aggtgtctct ttttcttttt cagacatgtc tttctctgac gtctttaata
    13741 cgtgtgccca agacttcatt tgattgatga actttttgct tttcaaatgg ataccgacat
    13801 attcatcata cagctggtcg atgagttcac ggattgcttg tttggtttct tctttcagat
    13861 ggattccact aattttatca taagaaatag ctgaaaacat ccgaacgaaa taaacagctc
    13921 gaggatctgc atgataacgg tgatcgtcca tcgaccaatg tctttcgcat atcacaccac
    13981 catatttgga agaatagtca aattttcctt ttgtttcccc gcatacgacg cagtgtgtcc
    14041 agattggtgc aattccgaag cgttgcagta tttggacttc aaaaatattc gtgatgattt
    14101 ctgcatcgtc tccttggtcc atccgttgca gtgcttgttg cgtaaactgg aacaaatgcg
    14161 ggtcgtactg atgatcttcg attgccgcat ctactagatt caataaatat gtgccatagc
    14221 cgctcaaaaa gatgtcttct tggatgtgtc gaaacgcatg gacatctttg acactgctta
    14281 aaaatgacaa accttcactt cgaaaattcc cgatataaac tgcttcagta aaagggagca
    14341 aagccggagc taagggctga ttttgccgat ggatctcttt gatataaaac atcattttgc
    14401 cagaagattc tgtaaagatt ttgactaatt tatctttttc tttgtaatta cggctagata
    14461 aaatcaagcc ttttgattct gcaagctggc tcatattctc cctcctaaaa aataaatctg
    14521 gaaaagcaga ttctgccgat ccagatcatt tatgttgcga tcttccgcag cttttctcaa
    14581 cacgcggatt tttattcagt ttatttctat acctaattta caagaagctc agaatatcag
    14641 tattcgtctt ttcggtagcc gaagtcttgc agatagactt ttttgtctcg ccagtctttt
    14701 tgaactttga cccaaagttc tagaaagact ttgtctccta aaagattttc gatatctttt
    14761 cttgctttcg ttcctacttg cttcagcatt tttccgcctt tgccgataat gatccctttt
    14821 tggctatctc tttcgacaat aatcgtagcc tggatatgga ttttatcatt ctcgtttcgc
    14881 ttcatcgaat caacaacgac tgcgacagaa tgaggcactt cgtctcgtgt taaaaacaag
    14941 actttctcac ggatcaattc agaaacgatg aagtattcag gatgatcagt cacttgatca
    15001 tctggaaaat attgtgggcc ctctggcatt tcactgacta atacatccat caaacgttcg
    15061 acgttgtttc cttctgtagc agagattgga atcacttgtg caaaaggcat ctgcgttgaa
    15121 taatcttcga tgattcccaa caactcgtct ggatggattt tgtcgatttt attgataatc
    15181 agataaacag gagaatgcac gttttttaga cgttcaataa taaaatcgtc tccccgaccg
    15241 cgtttttgat cagcactaat cataaataag accgcgtcca cttctttcaa cgcactgtaa
    15301 gctgcttcta ccataaaatc tcctaaacga tgctttggtt tatggattcc tggtgtatca
    15361 ataaaaatca actgagcttc tggtgtagtg tatactcctt ggattttatt tctagttgtc
    15421 tgtgccttgt cactcatgat ggcaattttt tgacccacga ttcgattcaa taatgtggat
    15481 ttccctacat ttgggcggcc gacgattgcg acaaaccctg atttatgttc tggcatatat
    15541 gttctccttt ttaattaaac aacaaatgat atattttagg gacaaataaa atcaacccaa
    15601 cgatgacggt aaaacaagcg gtcactaaaa ctgctccagc tgccatatct ttgatttttt
    15661 tcccaatcgg atgaaaatga aaatccgtaa acatgtccac gacattttca aaaacagtat
    15721 tgatgatctc tactatccat acgagaaaaa cagctaaaag cagccataac cattctatcc
    15781 gatcaagttg gaaaatcacg cccataaaca aagcgaggat tccgaacgca acatgtttcc
    15841 tcatgttgcg ttcttcctca aaaacagttt tgactccttg gacggcaaat tccacggaca
    15901 tcaggaaatg tttgtttttc tctacgtttt tatcttttaa gtccataggc atctaagatc
    15961 tctttctgta aaccaaacat ttctttctca tcctctggtg tcatatggtc atacccgttg
    16021 atatgcaaga atccatgaac cgctaaaaag ccaagttccc gatcatacga atggccgtaa
    16081 gcttctgctt gctctgcagc acgttcagta gagatcatga tgtctcccag tacacgaggc
    16141 aatggattct ctccatcttc aaaaatcact gggatttcgt cttcgccctc ttcttccaag
    16201 gcaaaactga tgacgtctgt cggtgcatct tttccacggt aatcacgatt gattacttgg
    16261 attgctgcat tgtccatgaa agtaacagac atttctgttt cttctggcaa ctctaaatat
    16321 gatgctgcaa attgcagcaa gtcgtcgatt tctttcattt tctcttctga gacttggttt
    16381 gtttcatcca tgaaggtaat atccattata catcgcttct ttctattctt cttgctattc
    16441 ttcttgttct tcttttatct tctcaacttc cgatttcatt ttaggatatt tgatccgaga
    16501 atggaaggta ctgcttaaac cgctgatcaa tgatttttct actttacgga tttcttttaa
    16561 cgtcaatcca ctgtcgtcca attgtccatc ggaaatacgc tcttcaataa gattatggac
    16621 aaaactttcg attttttcgc tcgttggatg gtccattgcc cgtactgccg cttcacaact
    16681 gtcagctata ttgacgattc ctgcttcacg gctttgaggt tttggtccag gataacggaa
    16741 ttgctcttcc gtaacttccg ggttgcgttc ctttgcctta acgtagaaat aacgcatcag
    16801 cgtggttcca tgatgctgtc tacaaatatc gatcaccatt tgcggcatat tataatcttc
    16861 caatattttg gcaccatctg tcacatgtcc aaagataata tgtttactgt cttctggcaa
    16921 taagaaattg tgcggatttt ccgctcctga aggcaagttc tcaacgaaaa agctggcgtg
    16981 tttgatcttg ccgatatcat gataatagca agctactcga gttagtaaag aacgtccacc
    17041 gatttctgct acagcattag cacttagatt ggctaccatc atgctatggt gatacgtacc
    17101 tggtgcttct tccagcaact gtttcaataa aggatgattg ggattgctga gttcatttaa
    17161 tacgatcaca ctatcatcat tgaccatcaa ttcaatatag gggtgcagac ctatcgtcag
    17221 caagaatgaa aacagtgcac ctgtgaatcc gcagactagc gttaaccaag tcgttgcatc
    17281 cccaaagctc attccttggt agataatcaa aatgacatcc atcatcacag gaaaaataat
    17341 gacccacata gcagcggata acccttgttc tgagatccgt ttgcgtctga cgatcgttgc
    17401 catgagacca gaaaatacat aggacatcaa aataactgtc agaaagttcg tgccaatcga
    17461 atcataaaaa atgaaaagtg cggatactgc ttgaaaaata ccagccatga tccctgctct
    17521 tcgattcaag aaaaagctta aaacaagtgg agcaaaagcc gcggggtaaa acagcggaat
    17581 ataagcagct tgttctgttt ggaagagttg gaaaaatttc attagtacga cgcttactga
    17641 cattgccgtc acgtagaaaa gtacgtattt tgttctttct cctgcatttt ggaattgaag
    17701 tgaattataa atcagtacac cgatttgaag caaaacagct aaaaccatgg caatgagtgg
    17761 gaaaatagaa gtcgtttgat tcgtcaatcc tagtaattcc aattttttca tcgctgaagc
    17821 atcaatttga tttccttcgc gaacaataat ttctccttgg aagatcatca ctggttgtac
    17881 agcttctcgt gcagattgtt ttaattcttc tgtctttttc tcattcaata catcattaac
    17941 tacgatgcct tgatccacta agtagcgaat catttgttgc tgctctgttg tgacattcaa
    18001 atattgaatc tgatcttctg cttcttgttt aaaagcttcc aattcgcttt cacgaacttg
    18061 tttactcatc tgttcgtcaa tgagcttcaa actttcatcc ctaactgtgt gaatttccgt
    18121 ttcagtcatt tcaaatatcg tcgaataaaa attgttcggc agtttttggt aaaacgcaac
    18181 gttttctgca ttcacgtttt caaaattttt cttcagtgta gcaatccgct cttctgtagt
    18241 aggtttcggg atattttcat cttccttcgc cttatctacc ttgccattat aagatttgtt
    18301 gatggtatca ttcgtcttat cgatcaattc gaataattgc ttgatccgat tgttctggtc
    18361 atctgccaga tctttttgat aagtatattc tggagtcact gcttctgctg ccagttttct
    18421 tctttgttcg gtttcttccg tgttctcaat cgttttattc gcacggatac tctcttctgc
    18481 tacttggcct tctctgaaat caatggcctt ttgtttgaca ctactaaatg taacgccgca
    18541 gacaatgata aaaaacaaga acagaaggaa aggcaatgct gcctttccga gtttttcttg
    18601 tagtttctgc agtgttttat tcaacatgtg cttatcctcc taagcaaagc ttattcttga
    18661 tgaagatccg ccttttcata agctttgatg atttcggcaa ctacagggtg ccgaacaacg
    18721 tcacttgctt caaaattaac aaaatcgatt tttttgattt gctttaatgt tcgttctgca
    18781 tgaacaagac cacttgtcgt cccacgaggc aagtctatct gacttgtatc cccattgaca
    18841 atcatttttg attgataacc gagacgggtc aagaacattt tcatctgagc gactgtcgtg
    18901 ttttgtgctt cgtccaagat aacgaatgca tcatccaatg tccgcccccg catgtaagct
    18961 aaaggtgcga tttcgatcac gcctcgttcc atcaatcggt tcgtatgatc caatccaaaa
    19021 atttggtata aagcatcata aacaggccgc agataaggat ccactttttc tttcagatcc
    19081 ccgggtagaa agcctaggtt ttcccctgcc tccacagcgg gccgcgtcaa aatgattttt
    19141 tgcacttcgc ctttttttag ggcagcaatt gctaatacta cggcaagaaa cgttttccct
    19201 gttccggctg gccctacacc aaatacgatg tcatgtttgg ctaccgattc aacgtatttc
    19261 ttttgaccgc tgttttttac ccgtataggt tttccattac ggtctttgac gatctcttcc
    19321 tcatacatct caacaaaata atctaatgta cctttttgcc ccattttcaa tgccgtcaca
    19381 acatctggcg tacttacgtg gattcctcgt ttgatcaatt cctgtaatgt ccgaatgact
    19441 gaggcagcca aggaaatctg ggattgctca ccaatgatct gaatgatttc tccacgtgta
    19501 tggatggttg tttccgttgt ttcctcgatc agttttatat gcttgtcgtg tgcacctaaa
    19561 agcatgctga catcatcagc agctgtcaat ctaatctcta aagaactaga ctcttccgtc
    19621 aaaaatagac atcccctttt tttcgattca ttctttccta ttttaccata ctcagaagaa
    19681 actatatagc aaataagaaa aggttgtaaa agagtcgctt aaccatacgt accaagaaac
    19741 gttaaaaaat agccgtttcc attcgctaaa ggtctcgttt tgataaaaag aagctgagaa
    19801 tgtaagttat atgaaagtgg agaacaaaat atttcgtttc attcaacaag tattctctag
    19861 gtatttttcc tgtttcaaaa aaagaaaaca aaatagttaa aatattcttg catttcaaaa
    19921 aaaacaggtg tattattacg tcaacaaata aacatacagc aaagcgtcga ggaaaacagt
    19981 aggctattca acgatttatt cccagtgagt catgtaagtg agaaatgaca ataaatcgaa
    20041 tagacgaaca tcatttccga gtcaatgtcg aaaacgtaga tcattgtggc tgcctccatt
    20101 atcaggctag cggataactt tatcaaaaag cgtccgtgaa tgaagaagct tttttaagcg
    20161 aaaaaaggtg gtaccgttct agaatctaga gcccttttcc caagtggaaa agggcttttt
    20221 tttattttaa ggtggtggtt ttcatggaat ctgatattag tggcattact aattgctgca
    20281 acgtttatat cgtcaatcaa aatgataatt attggaggaa aaatttatgg aagaaacaaa
    20341 aacttattca tggaaacaac tattgatggt cagttcactt atcttcggga tgttcttcgg
    20401 gtcaggtaat ctaatctttc ctgtccactt aggtcaaatg gctggcgcaa actgggtcag
    20461 tgctggcgta ggatttgcta tctcaggtgc tcttttccca ttactagcta tcttagctgt
    20521 agtagtgaca aaaagtgatg gtctgtatga cttagctaaa cctgtcggaa aatggtatgc
    20581 tgccttgttc ttaatccttg ttcatttgac gatcggacca tttttcggta caccacggac
    20641 agctgctact gcttttgaaa tggctgctaa accatttttg cctgaaaaat ataattcatt
    20701 aggtatgttt ttattctctg ctcttttctt tttacttgct tatttcctta ctgtccatcc
    20761 aacacggttg gtcaaatacg tcggaaagta tctaaactcc ttgtttcttg cattattagc
    20821 aatcattttt gtcgtttctt tattccatcc aatgggaaat ctaaatcaat ctgctgcaga
    20881 tacttatcaa acaaacgctt taacaaatgg tgtcctagaa ggttataaca cggttgatgc
    20941 agtagctttg cttgccttaa gtgtcacttt tgtccatgca gtccgtgacc ttggtttcaa
    21001 agataaaaaa gtcacagaat taacggcaaa agctggtagc ctgagcgttg cgctagaagt
    21061 catgatctat tttggacttg ttcttctcgg tgcgatgagt ttgaataaga tgtctttatc
    21121 agaaaatggc ggaactgcac tttctcagat cactaatttt tatttaggta acttcggcgc
    21181 tagcttctta ggagtgatgg tcacattagg tgtcttcact accgctatgg gactcgttgt
    21241 ttcttttgcg caagatttcc ataaactgtt ccctaaagtc agttatatga catggctgcg
    21301 gttaacgact ttcgtctctt ttgtcgttgc aaatgctggt ttagacaata tcatccaatg
    21361 gtctttacca gtattgatgc ttttgtatcc attgtcgctt gcgttgatct tgctttcctt
    21421 aacagcaaaa tttttccaaa agacaccatt tgtctatcaa gtaacgatgt tgtttgctgc
    21481 tgttcctgcc gtattggata tgctggcaaa ttcgcctgct ttagtgagtc aacaaagagt
    21541 tgtagcaagc atgctcgaat tttatcatca tcacgttcct tttgctgcat taggacttgg
    21601 ctggatggtc ccaacgttac ttggttacgc tggcagccta ctgttttatt acgcctatcg
    21661 cctatcaggg tataaacaag aagcaaatga actaccagaa gaataaccaa atcaaacaca
    21721 atttaacata agaaatatta aacaggaaaa aaccagctag atgaaagaat cctattcttt
    21781 cgtctagcta ttttttagtt ctctttttta cgcgcacaaa aaaactgcgg acaatgattg
    21841 tccgcagttt aaatgataaa attaagattg taatagttct ttcacaatgg catttacttg
    21901 gtttccatct gcttttcctt tgactttagg catgatcgca cccatgactt tgccaaattc
    21961 tttcattgat gaagcaccag tttgtgtgat tgcttcttga acaagttgtc gaatttcttc
    22021 gtcggaaagt tgttccggca tgtatttttc gactatcaca atttcacttt ttactttttc
    22081 agctaaatcg tcacgtcctg cttcttcaaa ctcatgcaaa gaatcacgtc gctgtttcat
    22141 ttcgcgagac aacaccgtta attcttcttc ttctgtcaag tcgtgacctt ctttaatctg
    22201 ctcgttttgt atagatgact tgatcattcg gatgacttgt aaagattcct tatctttcgc
    22261 tttcatcgct gttttcatgt catcgtttaa tctacttagt agtgacacac taccaactcc
    22321 aattcatcac agaactagaa ttttttgcgt tttctagctg cttcagattt tttcttgcgt
    22381 cttacacttg gtttttcgta gaattcacgt ttacgagatt cttgtaaagt acccgctttt
    22441 gacacggaac gtttgaagcg acgaagagca tcgtcaagtg attcgttttt gcgaacgact
    22501 gtttttgaca tgttaattcc ctccctccga gcttaaaaag taaccgtaat tgttattgaa
    22561 atatatctca aaaaacaaga ctgtcctgta aatattatat ctaatgctgt tttgaccgtc
    22621 aaggaaatct tgcttttttt taaaattatc aaatgttatt ttatttaaat ccttattgac
    22681 aattttcaca tcttccgaaa atctcgaatc tatgcccttc gatcgtacat ccaccgagct
    22741 gttcttcgaa ataattcatt ggacacatat ggatttcttt cgttgttccg cacaccgtac
    22801 agataaagtg atggtgatgg ccgatttccc cttgacagca atgaaaacga aacttcatct
    22861 ctccatttaa atcggtttct tccagtaaat cgatttctgc aaaatcatgg agatttcgat
    22921 aaatcgtatc gtaacttact cctttgaatt cctgattcat atagtcaaaa acttctttag
    22981 cggatacgta gcgattgcgc ttcactaaat aagttaacaa tgcttctctt tttttagtat
    23041 atttaaaacc gttatttttc aatgtttcta gtgattgttc aatcaatgtc tgttttttca
    23101 tgacttattc ctccaaatca aaatgattac gaagtacaca ttctagagta tggtataata
    23161 aacataaaaa aaatgcaagt gaggttttat ccatgtcttc cgattctaca gaaatcatta
    23221 ctttttttgt catctctgac tctgctggag aaactgcgac aaaattagcg caagccacta
    23281 tggcccaata tcctaccgtt gagttcaatt tattccgcag aacatttgtg acagatgaga
    23341 aaacattgat gcaggcactg caagatgcac taaaagaaaa agcccttgtc ctacacacat
    23401 tgatcaatca agaattgatc gagatgacgc gttctttttg tgaagaacat caattattta
    23461 gttttgatgt gatgactccg ccgatatcag aaatcgagcg cttaactggt atcaagccca
    23521 ttcggcaacc tggcgccctt catttattga atgagaacta tttcaaacgg atcaaagcaa
    23581 tggaatttgc tgtaaaatac gatgacggga aagatcctcg tgggtttcta gaagcagatg
    23641 tcgttttgct aggcgtatcc agaacttcca aaacgccttt gagcttgttt ttagctaaca
    23701 aaaatttaaa ggttgccaat ctacctttga tcccacaagc acatattccc aaacagcttt
    23761 gggagatcga tcctaaaaaa atcgtgggac tgacgaataa cccagatatt ttaaacaata
    23821 tccgtaaaga acggatgcgc tcttacggat taaatcctga tactgcttat tccgatatcg
    23881 aaaaaatacg agctgaactc gattttgcga atgatcttta tgaaaaattg ggctgcgtag
    23941 taatcaatgt cgcttcttta tctatcgaag aaacagcttc acttattttg aatgctttag
    24001 acctagaaga tcatagttat tatggaactg aaaccaccga agaaacagac taacagtttt
    24061 tcttgtcttt ttcttaaaaa aactggactg tgtcagcgaa gaagattcgt tgatacagtc
    24121 cagtttgtct ctatttgcac ctatcgttat tgatgttcca catgtttgag cgtctggttc
    24181 aaaatatcca gtacgtgaga atcatcaaga ctatacaaaa tcgcttttcc ctcacgatgg
    24241 gacttgacga catggttttc tcttaataaa cgcagttgat gcgatacagc agattgttcc
    24301 atttctactg cttgggtgat tgctgtcaca ttttgttccc cgtctttcag atacagcaga
    24361 attttgagac gagtggggtc actcaacatt ttgaatagtt ggctgacttt ctctactttt
    24421 gttttttgcg tgtctggcaa tccattcacc tccaacttga aaaaagactg acttctaaag
    24481 tttaccactt tttccatcag tctcaagttt ttttattctt ctgtcatttt tttgataatc
    24541 gcttcataag cggctacttt ttcagctgcg tcttctgaag agtttccttt tacaccgata
    24601 taaaatttga ttttcggctc agttcctgaa ggacggatag cgatccagcc attttcttcc
    24661 agcgtatact tcaatacgtc agatggcggt gtcgttagtt tttctacgtt tccttcacgg
    24721 tctgttctcg tcaactcttt gaagtcttct gtttggatga cttttgctcc ggcaaattct
    24781 gtcggtgctt cttcacggaa tttttccatc aaagcagaga tttttgcaga gccttcttgt
    24841 ccgctcattg tcacagaaat cgttttttct gcgtagtagc catatgtttc aaaaatcgtt
    24901 tgcaagccgt catacaatgt tttgccttgt tttttatagt aagctgctac ttctgctaat
    24961 aagagcaatg cttgaatcgc gtctttgtcg cgaacgaaag gtttaactaa ataaccgtaa
    25021 ctttcttcga atccgaacat aaaagtatgg gaatgatctt cttcaaattg ttgaattttt
    25081 tcagcaatga atttgaatcc tgtcaataca ttgaacatgg tagccccata gtgttccgca
    25141 attgctgtag gcaattcgct agaaacgatt gatttcaata ccgctgcatt ttcaggtaaa
    25201 gtgcctcgcg attggtgtgc ttccaaaata tattgagcaa acagggctcc gatttggttt
    25261 cctgtcaata cttcatagga gccatttggc aaacgaacgg ctgcgcctaa acggtctgca
    25321 tcaggatccg ttgcaatcag taagtcagcg ccttctttct cgccaagtcg catcgcatac
    25381 tcgaaagcag agtgttcttc tggattcggt gatttgactg tagtgaaatc cgggtctgcg
    25441 acagcttgtt ctggaactag tacgaattgt tcgaatccag cttgtttcaa cgcacgttcg
    25501 cccaacattt ttcctgttcc gtgaagtggt gtgtacacaa gcttcaaatc tttacccatt
    25561 gtttccacca attcatgatc aatcgttact gctttgattt cttctagata agcggcatcc
    25621 acttcttctc cgatgatcgt gatcaatccg ctatgttcag cttcgtcgtc agataatact
    25681 tcaactttta acggattgct gacttcacgg acatatttcg tcaatgcatc tgcgtctgca
    25741 ggaggcattt gtcccccatc ttctccgtag actttatatc cgttgtaagc tgctggattg
    25801 tgggatgcag ttaccatgat tcccgcaaac gtcttcaaat gacggactgc aaaagaaagc
    25861 tctggtgttg gacgtaaact ttcgaataca taagaaggga tattgtgatg tgctaatgtt
    25921 ttggctgctt ccatggcaaa ttctggtgat tgatggcgtg agtcataagc gatagcgaca
    25981 cctcggcgtt ttgtttcttc accttgtgta tccatgaaac gtgcaagtcc ttctgttgct
    26041 tgtcttaccg tatagatgtt cattcgattg atcccaggtc ctaagattcc ccgcatgcct
    26101 gcagtaccga attccaacgg tgcataaaaa gcatcttcaa gtttttcagg atcttcaccc
    26161 agatcattta attgtttttt taagttttct tctaaatttt cttcatgtac ccattgttca
    26221 tacacttgtt cccaagacat gaaaataaca cctctttctg aaatatggtt cttaataagt
    26281 atagcattca cttattagat atgaaagtat gaggaccaaa aaacgcttaa atcaagaaaa
    26341 gattctttta tcaagcacgg ttacaagtat tcgcataaat cggattcagt agatttcacg
    26401 caaaaaaaag agtaaaagaa caattgaaac cgttctttca ctctttttaa acttatgcag
    26461 ttgtttcttc taaagattcc aagtatttat acacttgttg tccagcaatc ccaccatcac
    26521 caacagcagt agtgatttgt cgcaaatctt tttcacgaac atctccgatg gcaaaaacgc
    26581 ctggtatatt cgtcttcatt tcgttgtctg ttggaatcca gccggcttcg ttcgtcaacc
    26641 cagcttctct aaatggtgca gttaatggat ccaatcctac atagatgaat actccgtctg
    26701 ctgccaaatc aaactcattg ccagttttga cattacgtgc tttcacacct gtcactacca
    26761 tttcatttcc aacgatttcg tcaacgactg tatcccacat aaaggaaatt ttttcattcg
    26821 caaatgcacg gtcttggatg atcttttgtg cacgtaattc atctctacgg tggacgatga
    26881 ctacttcaga agcaaattgt gtcaaataga tggcttcttc tactgctgag tctcctcctc
    26941 cgatcactac tagacgtttg ttacggaaaa atgcaccatc acaaactgca cagtaagaaa
    27001 cgccacgtcc agcaaattct tcctcgccag gtacgcctaa tttacggtgg atacagcctg
    27061 ttgcgataat gactgttttt ccttcgtaag ttttatcttc acaaataact tctttgtggc
    27121 tgccgtgatc cttgatatct tgcacgattc cataagcatt ttctgtccca aatcttgaaa
    27181 caccttcata catcttataa gctagatcag gacccatgat tgaatcgaaa ccaggatagt
    27241 tttcgatttc tgccgtattg ttcatttggc cgccaggagc gccttgttcg atcatcaata
    27301 cggataagtt tgaacgtgaa gcatataaag cagcagtcat cccagcaggc cctgcgccaa
    27361 taacgattac atcatacata tgtagattct cctttactta atcaagtaag catactttac
    27421 agccaatgcg aaaaaaagtc tattgttctg cttgcgtttc tcgaatagct ttattatact
    27481 cgaaaaatcc gctgaaaaac aatagacttt tataaatcat tgatcaatcg ttcgaaagat
    27541 tttctccatt tgaatcaatt acttttttat accagttgaa tgatttcttc ttcgaacggg
    27601 ctaatgtgcc atttccttcg ttgtcacgat cgacataaat gaatccgtac cttttcttca
    27661 tctcacctgt accagctgat actaaatcaa tacatcccca agtcgtatag cctaatagat
    27721 cgactccatc taattctact gcatctttca ttgcctggat atgtgctgcc agatagtcga
    27781 ttcgataatc atcttccaca taaccattct catcgggcgt atcgactgca cctaagccgt
    27841 tttctacaat aaatagtggt ttttgataac gatcataaat atcattcatc gttattctca
    27901 agcctaatgg atcaatttgc caaccccatt cacttgcttc cagatatgga tttttgacag
    27961 aagcaaagat atttcctgca gtctgttcca agagttcgtt gtcagtagtc gaaactcgag
    28021 aagaataata agaaaaggag ataaaatcaa cagtatgttc tttcagcaat gcggcatccc
    28081 caggttccat tttgatctga atgccatttc gttccatttc tttcaatgca taagcgggat
    28141 attctcctcg tgactggaca tcaatgaaga aataattttc tcgatctgac tttctagaag
    28201 cccaaacatc ttcaggattg caagtgtatg gataatatgc tcctgctgcc agcatacagc
    28261 ctactttgtt ctccggatct acttcatgag cgatcttcgt tgcgatggca cttgcaacca
    28321 actcatgatg tgccgcttga tatttgattt gttcttcatt ttctccttct tcaaaataca
    28381 gccctgcgcc catgaatggt gcatgaagga tcatattgat ctcattgaat gtcaaccaat
    28441 atttgacaag tcctttatag cgattgaaga tgactcggca cagattttca taaaaaccaa
    28501 cgagttctct ggaacgccac gcaccatatt ctttgatcaa atgcatcgga caatcaaaat
    28561 gtgtaatcgt cacaagcggc tcgatcccgt acttttgaca ttctttaaag acatcctcat
    28621 aaaatttcaa cccagattca tttggctctt tttcatcgcc cattggaaaa atacgactcc
    28681 aagcaatgga catccgataa gtcttgaacc ccatttctgc aaataaagca atatcttcct
    28741 tataatgatg atacatatcg atcgcaattt ttgctggata aaaatgattt tcatcaaatt
    28801 caaacatttt cttttttcca gtaatgacag gaaaacggtc ttctccgatt ggcacaacat
    28861 ctacgttcgc taacccacgt ccgccttcat cataaccgcc ctcgcattga ttggctgctg
    28921 tagctccgcc ccataaaaaa tcttttctga atcccatcat acttcctcca ttacatgtgt
    28981 attgcttgat aattctcttg ccagttcatc ttggacttcg attccttctg cttttaaccg
    29041 gctgattgtt tccttgaatt gcggtaaata ctgctgatgt gcaataaaca actcatccat
    29101 gatccgtttt gctgtctttc ctgatcgtat aagcggattg atcgtaaacg cttgcaaagc
    29161 tgttccataa tcaccagtca cggctgcttc gatcgtcagc aattccatat tcttcattac
    29221 ttgcaaccag cctctttcag cagcaggaag ggaaccaaac gctacatttc gtgcgccttg
    29281 tgcgccaaca taggcagtga cttctactac acaatctgcc ggcagatcag gaacagctcc
    29341 atcattcttt gtagatacca cgatctgtgt ttctttattt ccatagatcg aagcaatcgt
    29401 ttcacaggca gcatctgaat aataagcacc acctcgtttt tgcaattgtt caggtttatg
    29461 atccaattct ggatctttgt ataattcaaa cagctctgct tctgtttctt tcacttgctg
    29521 cgctcttgta ccaatcgttt gatattcttc taaagcatgt tccagcattt cttcttcacg
    29581 atagtaatag cggtggtagc cacaagggat catgttcatt tgttttaatt gttctttgaa
    29641 aaacgggatg tcaaagatat ttttcggcaa tccattgtct tcatcgtata aacgatcaat
    29701 caattctgcc gtgcggtcat ttcctttatt atctgctact ttgtgccaat ggaagtgatt
    29761 tagaccggca aacttgtaga tcagctcttc ttttttcttg ccaagcattg ttggttctgt
    29821 catcatcgcc attactggca cattgcataa tccgatcact ttatcccatt tgccatatct
    29881 gataactgct tctgtcacca tcccgcttgg gttcgcgaaa ttaatcagcc aagcatttgg
    29941 acataaacgc ttcatgtctt caacgattgc caaaaccact ggaacggtac gaaaagcttt
    30001 gaatatcccg ccagcaccat ttgtttcttg cccaatcatc ccataataac tagggatccg
    30061 ctcgtctttg attcgtgcat ctaattgacc gacacgaaac tgagtcgtaa caaaatccgc
    30121 atctttcaat gcttcttccc gatccagagt tgcatagatt ttgacttcat acggagaagc
    30181 atcccacata cgctgagaca tctgactgac gatctcaagc ttttcttttc cttcttctac
    30241 atctacgagc cagatttctc gtatcggtaa ttcctcatat cttttgataa atccttccat
    30301 caactcgggg gtgtaactgc tgcctccacc aatcgtagcg attttgattc cttttgccat
    30361 gttctcactc ctttactttt tacactttta ttatcttttt tgtaaacaaa acaaacaata
    30421 gaaattcgtt tctttccagt aacatttaca aaagttagtg ttttcttctg gatttttgta
    30481 aatatcttgt caaaaaatgt ctttattaag ctatataaca taaatccgtt tacaaatcat
    30541 tttcaaaaaa gttataattt acgtaaaaaa acaagaagaa ggtatctaca tgttattacg
    30601 tgaaaaaatc aaagaaatct ccttttctcc ttcagaaaat gaagtgattc agtttctctt
    30661 aagcgattct ccagcacttg gcgaaatgac catccaagag attgctgagc gatgttatgt
    30721 ccacccgtcc acactcatcc gtgtagcaaa aaaaatgggt tttgatggct gggtcagttt
    30781 tcgtaatgct tttttagctg aaacagccta tcttgaaggg aattttcaag acgtagatgc
    30841 gaatcttcct ttttccacca cagacggtat tatgacgatc gcaaataaaa tggcttcttt
    30901 agaacagatg acgattcaag atacactttc tctcattcat catgaccaac tgcaaaaagc
    30961 caaacaacta atgcttcaag caaaaaaaat cgtgattttc gccagtaact cgaatacttt
    31021 gatttctcaa gatttcatgc tgaaaatgaa gcgcatcaaa atggatgtca cgattgtcac
    31081 cacttttggt gaatcgttct atgaagcgta caattgcaca agtgatatgt gtgggattct
    31141 gatttcttat acgggtgaaa acaatgtgat ccttcgtacc gcagaaatat tgaaacaaaa
    31201 tcatgtgcca attttagctt tgaccagtat cggtgattca acgctatctt caatgagtac
    31261 agccgctttg cgacttacga cacgcgaacg actgtattcg aaaattggta attttacgat
    31321 caatacatcg atttgttatc ttctagacgt gttatatagt tgtgtttttg cagaaaatta
    31381 tcaacagaat ttagtccatc taatttctgt aggacaaaaa tttgataaac gccctatcag
    31441 ctcagccatc atgaaagagc ctttcaattt tgaaaataaa gaagtggact aaaaagaata
    31501 gttaagccta gtccattctt tcacctaacc gttcaggtca gtgcggtgat aagtgcatcg
    31561 tctggcccga ccgtttcccc ttgattttcc aaaatatcca aataatccat gtaattgatg
    31621 acaatcacag gtgtttctaa actgtatcct tcctcttcga ttttcttttt gtcgaagcga
    31681 agcaacagat cgccttgttt cactctttct ccttgctgca ccaagctttc aaaatatttc
    31741 ccttccaact gaacagtatc caatccgaca tgaatcagca attccagacc tgcttcagaa
    31801 accatcccga ttgcatgttt cgtcggaaac agggacatga ctgttccgtc aaaaggggca
    31861 cgtacttctc ctttttcagg gtagatcaag attcctcgac ctagtgttcc ttgtgcaaaa
    31921 gctgcatcct ttgctgcatt cagtggagcg atttgtcctt gtatcggtgt ttttacaatt
    31981 tctttacctg atcttttctg tctatcatct gtttcatctg cttcctcctt ccaaaagaaa
    32041 aaagtcagcg caaaggatac caccatcgca atggcgatcc caatcaatac attaatcaca
    32101 tctgttaaat caccatctgg tctaatatag ttgggaattg taaagatgcc tagtccacct
    32161 tgattccaag aagttaaatt cattaaccca ctatacgcgc cccctaacgc accaccgaca
    32221 caggtaaaaa taaatatttt tttacgtggc aatgtaaacc cataaatagc tggctctgta
    32281 atccccacaa taccagaaac aatggctggt attgcaattg ctttgtcttt tggatttcta
    32341 agcttaaagt agagtgcggt aagtgctgca gtttgtccaa aagacactac tgcaacaggt
    32401 gtcataatat tttgccaacc attcacagaa aattgcatca atcccattgg tacgattgcc
    32461 caatggagac caaacatcac cagaatttgc cagaagaagc ctacgattgc tccataaata
    32521 actggcgaaa gtgctaaaag tgtttcaaat ccagccatta aaatatcagt tgcgaatgtg
    32581 aatactggtc caacgagtaa aaaacctaaa ggcattgtaa taagaacggt gaagaatgga
    32641 acgaagaaat tcgcgatcat ctcaggtact aactttttaa ttggtttctg aatttggcta
    32701 gctaacaaca caataaagat aatgggaatg acagaagaag cgtagttacg cgcaatccag
    32761 ggaatgccga atactgtttg ataaataggt gcttcaaaaa tagtgccaga aaatatggtc
    32821 gttagtggtg cttctgctgc accactcaaa gcttccagcg acaagctagg attcattaat
    32881 gctgcgccaa gcaacatccc tacgaattca tttactttaa atttacgggc agctgttaaa
    32941 ccgataataa caggcataaa ttggaataca gaatcaccga tattatcaaa cataacaaat
    33001 gtgccagaac cgcggtcaaa cgcgcctaag aagacaagga atgctgtcag tccacgaatc
    33061 atcccgtcag cagctaggat agcaaggaat ggttggaaac agcccgatat tccatcaata
    33121 agcatatcaa acggtctcat tttttctgtt gaaacagcct catcaaaagt aaggtttcct
    33181 gcttttataa cctcttcaaa cacgactggc acatgattcc cgatgacaac ttgatactgc
    33241 cctcccgctc gcattaccgt tacaataccg tccatatttt tcaagatctc atcatttgct
    33301 ttttcttcgt tttttaattt gaaacgaagc cttgtgatac aatgtgtcaa accattgata
    33361 ttttccttcc cgccgacatt ttctacgatt tttctcgcta attcttggta ttttcccatt
    33421 tctattccct ccaatttatt agtggaaggt ttagccaact gaatgtcact acccttattt
    33481 catacgttct tgttaattta tcctcctttt tttatttatt tacaacgatt ctcatgcact
    33541 aaacgcgcag tatggatcgt taaatacatt ttttcgtccg tggacatctg gtagttgaac
    33601 gtctcggcta aaaagtctgc tattttctca acgcaaccaa acgtatcctt atattttttt
    33661 tgaataacct gaaacaattc ctcagaaact tctcccactt ctgatttttc tccatctagg
    33721 acgcgtgtca gaaaaaaacg taagtgtgtg acgaatcgaa aaaaatggac tgaatcttca
    33781 tcaaatgtaa tcttatagta atagcggacg atatttagaa tatcctgtat tgttatagtg
    33841 aacaggtagg tattgtcttg ttggctttga atgccttgtg cattgactaa atgcaatgcg
    33901 ataaatccgg cttcatcttc tgataacttg atgtggtatt tatccgagat tttcttcact
    33961 gttttttttc ccgatggcat actctttagg aaatagttgt ttgatatcat acagcaatac
    34021 atttggtaca aatattccca ttttgtgacg ttcgatagcc gtatacaaat gatccgtcaa
    34081 tgcaatatac acagaatcat tcaactggac ttgtagagcc tcttttgtat attcaatgat
    34141 ttcacaagaa atttctaagt actcaagagg aatcgcttgt gccaattctt ggaactgttg
    34201 tgaaagcccg tcatttgtca actgaaattt tttgtcgatg catgagtcaa aaatcgggtc
    34261 acccactttc tttttgaatg ccaaaccttt tcccatcacg atcatttcct gatttctttc
    34321 atccctggtt attacgacgt tgttattcag tactttctca atcagcatgt tttgctcctt
    34381 tccatgatat atagggctgt atccaaaaca aaaagaaaaa cccaaaaagc cgtccatgga
    34441 accatgggag actcatcagg tttagcttgc atcaggcaat cactatccaa gttgtatcgt
    34501 tttctttata ttatcgaaag caaacgcttt tgtcaacgct ttcttagtgg ttattagccg
    34561 cttttgacta gctgtcgtcg tcttttttaa atcgggtgac tacgccacta acgctcacag
    34621 atgaagaatc tgcgaagctc tgttttattt tcactctagc gcttagggca aaatgctact
    34681 atgctactgt cacaacctct ttttacacac cactgctttg cagtttgtac atatctgcat
    34741 acaaaccatt ttgcgctaga agtgtttcat gattcccacg ctctactata tgacctttgt
    34801 ctaaaactaa aatcaattct gcatcccgaa tcgtggacag ccgatgggca atcgctatcg
    34861 ttgtccgtcc ttgtcgcatt tttgccaatc cttcttggat caatccttcg gtttcagtat
    34921 cgatatttgc tgttgcttca tccaatacta gtattttagg atctgtcacg attgttcgcg
    34981 caaaagtgat cagctgtcgt tgtccactag aatagctggc accgtgttcg atcacttttg
    35041 agtgatatcc ttttggtaat tcatcgatga acttgtttgc ttgaacaaac tcagctgcat
    35101 ggcgaatctg ttcgtctgtg atagctggat tcatcatccg gatatttcct gcaatatctc
    35161 cataaaacaa gaaggcatct tgcaagacca atcccatttt cttacgtaac tcttccattg
    35221 gatattctcg aatgtcatga tcatcaatca agatttggcc ttcataaaat tcgtaaaacc
    35281 gcatcaatac gttgatgatc gagctttttc cgcttccggt gtgccccact aatgcaacgg
    35341 tctcacccgg attagcgaca aaagagatat tgttcaatac attgtgctct ccatcataag
    35401 aaaagcttac attgcgaaac tcgatcttac ctgaagtaat cgcttgatct gctccaggat
    35461 tttgttgcgg tgcatattct tctgtatcca tgattttcaa aatacgacta ccggcaacca
    35521 tcccatctgt aaaggtgctc aaaaagtcca tcatttgaga cattggatta aaaaattcct
    35581 ggacataggt cgtaaatgca tagatcagtc caacttctac gggagaatgc aacgcatcga
    35641 tcccaaacaa agtcaacgcc aacgcaatcg ccaatgcata gagaaaattg atgattggac
    35701 ttaacagaat cgaattcgtt ttgatcatcg catagcgcgt gtctaaatac tcgttgttca
    35761 cttgttcaaa ttctttagct aaacgagctt cttggcgaaa ctgctggata atacgcatgc
    35821 cggaaatcga ttcattcaat ttcgtgttca gctgacttaa tttttcccgc atatttcggt
    35881 aaatacgaga actgaatttc tggtaatacc agatgacgat tcctaaaatc ggcaagaaca
    35941 ccaaagtcca caaggcaatt tctccattga tttgaaacat ggcaacgaaa gaagaaacga
    36001 ctgcaaagat cccagtcaat accatcaaaa agacatacca aaattcaaat agcgtttctg
    36061 tgtcattcgt cactcgagaa acggtcgatc ctgccggtgt ctggtcaaaa taacgcatac
    36121 ctagtgtatg cagcttttca aataatctta cccgaacata ctgataggtc ttcaatgaag
    36181 ccatggaata caaataccat tggaaaaacc aaatgatact tttgactatg actccaaaca
    36241 agtacacacc agcaaaaaac aaaataactt gtgtcgtagc tgactgttta gccagatgat
    36301 catccataaa agtttggatg atccttggca ataggacatt gatcacagac agggcaaacg
    36361 caaataagat agcaacgatg aacgttcttc taaaaggttt ggcgaacttc atcagtcgtt
    36421 tgatgatctt cttctgttct ttaagtggaa tcgattttgt ccattccgat tgataatttt
    36481 catccatcag gcctcgcctc cttctatctt cgcttctaat tgctgttttt cccacattcg
    36541 ttcataccag ccacctaatg caagcagctc gtggtgggtt cctcgttcaa tgattttccc
    36601 ttgatcgaca acaatgattt cttttgcatg catcacgctg cttaaacggt gagctgcaat
    36661 gatggttgtt ttctcttttc ttatttcttt taaattatgc aagatagctt cttctgtttt
    36721 ggcatctact gcagaaagtg catcatctaa gatcagcaat tcaggatttt tgatcaatgc
    36781 tcgagcaatc gaaatcctct gtttctgtcc gccagataaa gagactccac gttctccaac
    36841 taaggtatca taaccttttg gcaattcctt gatttcattg ttgataaatg ctaaagaagc
    36901 cgcatgctct acttcttctt ctgaaagttt tggattagaa aaacggatat tatctcgaat
    36961 ggtcatcgaa aacaggaagt gatcctgagg cacataaccg attgatccta ataaagcatt
    37021 caatgaatat tctttgatcg aacgtccgcc aaattggatt ttcccttgat aatcatcgta
    37081 ttcacgcatc agtaatttga tgattgtcgt ttttcccgca ccagttttcc caacaatacc
    37141 taatgtttct ccttcttgga tcgcaaattt gatttgttct aatgctggtg attcttcttt
    37201 cggatagtta aaagaggaaa tagctacatt cagctcgcct tttgccgggg tctgcactgc
    37261 acccagttgt tccacgatat gcgtcttttc atgcaaaagt tcattgacgc gatcataaga
    37321 agcatttcct ctttccaata cgttgaacaa tcgaccaatc gcaaacatcg gccaaacaag
    37381 cataccgata tagctaataa atgagaccat ctgcccgatt gagatcgtgc cttccaagac
    37441 atactttcca cctaaaataa ttgttgcaac ataagacaga ccgataatca aggtaatgaa
    37501 tggatcaaat aaggagtcta aaaaattgac tcgtttgttt ttaataattg catcttcaat
    37561 cttttcttca aaatccgcga gatcttcttt ttcttgtcca aatgttttaa tgactttgat
    37621 cccagtgata ctttcttgtg tcttatcatt gatactagaa aaagcttctt gcgaatcacg
    37681 aaaagcatcg tggagttttg accccagcac tcgtgaagta acggctagca aaggcagagg
    37741 aatcagcgca atcagtgtta agcgccaatc aataaacaaa atcatcgcaa tgatcgttgt
    37801 tccgcccgta atcactgaat ctgcaaaggt caagattcca gctcctgcta cattctggat
    37861 tgcattcaaa tcattcgttg catgagccat caaatctcca gtccgatatt tttggtaaaa
    37921 gagattgtcc atttctgtaa aatgtttgaa tagctgctgc cgaagttctc tctccaaccg
    37981 agctgcactt ccccaaatat tcgtccgcca gatataacga aaaagatatt gtaagaaagc
    38041 tgcaaggatc aataataaga tccaccagga aatgatcttt attgataaat tcttatcggc
    38101 gatctcatca atgatgatcc caatcacttt tggaggaact aactgtacca aggccactgc
    38161 gaatagggag aaaatcccaa ttaaataatg tttcttttcc tgacgaaaaa accaacctaa
    38221 ttttctaaaa atcgacattt tactcctcct ttgtctttat caagagcata ggattgtctg
    38281 tgcttgtctt ttttgctgaa tagtctgaat agtctaacaa attacgaaat aaaaaacgga
    38341 ctgaaaaaat cagcccgcaa aaagtctttg ggtcagaacc tattttttcc cttggttctt
    38401 catttgctgc atcatctggc gaattttctt ctcagatggt ttttgcccca ttgacatcat
    38461 cattgatcgc aacatgtcct cattaacggg aggatttttt tctaaatagt ctttcatata
    38521 tttacgcgct aggaaaaagc cacctactgc gccgattagc aaagcaataa tagcaataag
    38581 tacgacaata cctgttgata ccatataaat ctctcctttc tatttccgaa ttgctctcaa
    38641 tgatttattt tactaaagtt tattgaaaaa agaaaggcgt attctttagt ttcatgcaaa
    38701 aaatagtggt ttttttagag aagaacaaaa tttctttcat taaccagcaa gacacaccac
    38761 gaaaaaaaat taggcatacc gaatcttttt ctttaatttt ttctttctga ggtgtagaat
    38821 gtaatcgtag ataaatagga aaggtggttt tttcttgaat aatcaatcca acaaacaatt
    38881 tcataagcat cgacttattg aacatacaaa tggtttatct ttagctgaag taaatggaac
    38941 agtggatgta ccaaagggaa aaagtttttt taaaacactt cttgcttact ctggaccagg
    39001 tgcattagta gcagttggtt atatggatcc aggtaactgg tccacttcga tcactggtgg
    39061 acaaaatttc caatatttat tgatgtcagt tattttgatg tctagtctaa ttgctatgct
    39121 tttacagtat atggcagcaa aattaggaat cgtgacccaa atggatttgg cccaagcaat
    39181 ccgagcacgg acaagtaaga cattgggtgt cattttatgg atcttgactg aattagccat
    39241 tatggcaaca gacatcgcag aagtcattgg tgcagcaatc gccctctact tactttttga
    39301 tattccattg gtgattgccg tctttattac tgtattagat gttttcgttc tcttactact
    39361 gacaaaagtc gggtttcgta agatcgaagc actggttgtc tgcttaattt tcgtcatttt
    39421 attcgttttt gtctatcaag ttggcttgtc taacccagat tggcacagtt tatttgctgg
    39481 ctttatccct aacagtcata cattctcaga ctcaccggtc atcggcggcg aaactccgct
    39541 gactggagct ttagggatta ttggtgcaac agtcatgccg cataacttat atcttcattc
    39601 agcagtttca caaactcgga agatcgatca ccaaaacgaa gaagatgtag cacaagctgt
    39661 acgtttttct acttgggatt ctaatatcca gttgtcaatg gcttttctag tcaatgcact
    39721 gctattgatc atgggtgtcg ctgtcttcaa aaccggttct gtccaagatc cttccttttt
    39781 tggtctgtat gaagctttat ctgatccatc agctatgagc aatggtattt tgattgctgt
    39841 agcaaaatct ggtatcttgt ccactttatt tgctgtagca cttcttgctt ctggccagaa
    39901 ctctactatt actggtacgt tgaccggtca agtcatcatg gaaggattca tccatatgcg
    39961 tatcccgatt tggctaagaa gactggtcac acgattgctt tccgtgattc ctgtattgat
    40021 ttgtgttttg tttacaagta caaaaggaac gatcgaagaa catgccgctt tgaataactt
    40081 aatgaatgaa tcccaggtgt tcttggcttt tgcgttgcca ttttcgatga ttcctttact
    40141 gatgatgacc aacagccaag cggagatggg tagccgattt aaaaattcat tcatcgtaaa
    40201 acttcttggc tggttctctg tgattagtct aacttacttg aacttacgag gattaccagg
    40261 acagatcgaa agtttcttcg gcgagcaagc cacttccgct caactagcat tggctgatca
    40321 catcgcttat gtaatcattg ttcttgtcct tctcctgttg gcatggacga ttggtgattt
    40381 atataaagga aacaaaaaat atgaacagct tttgcaggaa acggtagaag aaatcgaata
    40441 aaaactgatt tttccacaca aagacccttg atagttaggt taaaaatcta gctaccaagg
    40501 gtctttttat ttagaagata aagctttttt cagatttaat ttactgaaaa ctagcatgct
    40561 tgttactacg atgaattgcg ttaatggcac gcctgcacca gtgtagacac ttaaaaaata
    40621 gtcctttttt tctggcgcat aagaacaaaa ccatgagaca ccgaaaagaa aaaccaattg
    40681 tgtcagtatt aatccggtga ccacaatcaa agaggaacta taataggcac gatcagcttt
    40741 tgcaaatacg gttcctagta tcataagtaa tatccctgct attgaagcaa tcagcacggg
    40801 aatagccaga acttggtaaa tttttatgat gacattcgcg acttttgctg attttgttaa
    40861 ttggaaatta ggtgttctcg gttcattact atcgatccaa ttctccatta gcggaaggtt
    40921 taataattct tgagcctgtt tgacttgttc ttgcgttccg aaactataat ttcctccttg
    40981 atggaaatct ttatatcgcc atgcataagc gtatcctgat tgaaggaaat cgaatacgat
    41041 tggcagatca ctcatcgtct ttccatctcc tgatcccatc agatagaact cttttttctt
    41101 tttgatggtt cccttgtgat aggcttcttc taattcttgg ttgacttttt tccaaaaatc
    41161 attggtttct tttgcattgt tttggtaata accagattcg cttaccacat ctcgcaatgc
    41221 ccaaaagatg atatctccgg gtaattcttt tacttttcca ccttgagtcc acgcgtgagt
    41281 cgtatataat tcgtcaatac gggtagaaac agtatggaat gtaggagatg cggctaatgc
    41341 tttatttagt gcagcatgtg agacccatat ctcactgttt tctttattca agttcgtttc
    41401 atcatctatt cggatcagtt gtttcatcaa acgtccaaat tctccactgg aacggtcatt
    41461 gatggttgca ataccgtatg ttgtctcatt tttatattcg atccactgat agacgatgga
    41521 aaagccaact ataggaagta aaaacaatga aagtttcagc cattcccgtt tcgagagaag
    41581 aaagagtcgt ttttttaact tctttacttc tttgaaagaa aagtcttgcc ctagcaagat
    41641 ctggacgaaa ctgataccca aagctactac agtaaaagga agaagccaga tagaatcttc
    41701 gcgaatgaac caaaagaaag gaaaagcgac agaaagccct aaaaaccatg gcagcaattt
    41761 tctgctagaa ccgtatttgc ggtaataaat agctaataaa caagcgataa tgatctcaac
    41821 agccggaatc acgagggcat tacgatatgt tcgagtggaa tattcatgtg taaaactaat
    41881 cggagaaaac aaaaaataaa aatagatact taaaagaaga tacttgttct tcacaatcgg
    41941 tttaaacgcg agtgcaacca atatactggc aaaaatactg aacatgccga acaaaactgc
    42001 ataagaaaga tgcagtttat gtgcaagtac taaaaataag ctgtaggaga ttcctttgga
    42061 aagtgttgta gtcgtatact cccctagcca tttaccatcc aaaatatttt gagcatagtg
    42121 aatcagcagc tgatcatcat aggtcgccga catatctgtt ccatagattg ctttactata
    42181 taaatacaac cgaataattg taaataatac ggctatgcca agaataaagt atgtatgttt
    42241 cttttttagt tggtcgatca aggggtttaa aatgttcagc atcttttcgt cctttcaatc
    42301 acgttttgct acttgtttgt gaaacttttc gttttttact tttctatcag tataccctgt
    42361 ttgtctgaat ttaaaaagag ggggcaaaag gagcatggcg aaaacgaatg caaaaagcac
    42421 tcctttgttt cttaaacaaa aaaggacaag gcagactgcc caatcctttt ttgttgctgt
    42481 caggtagaaa acttaaagtg tgacttcttc tgctaataac tcagggaaca cttcgggatc
    42541 gaatgcttgc gctttgaaca tggaaatttc ttttccatat ggtgcgtaag ctttcttttt
    42601 atctttaccg acatatgggg tttctaatat tttcgaaatg ttgtcaaaca ttggatcatg
    42661 gacgattttg ttcaatgtgt caaatccaat tgttccaaaa ccgatattgg catgccgatc
    42721 tttatgagaa ccttgtggat ttttagaatc attgatatgc agtactttaa gccgatctaa
    42781 tccgatgacc ttatcaaact cttccattac acctgaaaaa tcttctttta tattatatcc
    42841 ggcatcattc gtatgacatg tatcaaaagt aacggataat ttttcattca attttactcc
    42901 atcgatgatt tgcgctaatt cctcaaatgt tcgaccgatt tccgtacctt tccctgccat
    42961 cgtctccaaa gcgatctgtg ccgtctgttc ttttgtcaaa acttcattca acccctcaat
    43021 aattctagca atcccagctt ctgcccctgc tcctacatga gcgcctgggt gaagggtgat
    43081 ttgagtagca cctaaagctt ccgcacggac aatctcttct ctcagaaatt gtacagcaaa
    43141 tggaaacatt tctggtttga tcgtatttcc caaattaatg atataaggcg catgaacgac
    43201 gatatttgtt agtccgtttt ctgccataaa tgcttttccg gcatcaatat tcatctcttc
    43261 gatagctttt cttctagtat tttgaggagc gcctgtatag atcataaagg tcgaagatcc
    43321 gtaactggct gcttcttctg ccgcgcctaa cagcattttc tttccgctca tgctgacatg
    43381 tgagcctaat aacatggatg gattcctcct tgaaaggcaa ttgtttcgaa caattcctgt
    43441 atattgataa gatgatgtac aaagaaaatg gtttgttttt ctttgccctg ttctccatag
    43501 taactgaaac tttccctaaa gaaaagctta agaactcttt cataaaaaag atcatttcct
    43561 cacgttttat ctcattcgaa aaagaaattc ctgtcaaaaa cagccttgtc tgtttttttg
    43621 tcctattatt tgctataatt agcgaaataa aaccattagt tgaggtgacc cttttgccac
    43681 gcaaaaatac tcgaaaaaaa cagaatagaa gaaaaaaaga aaaatggttt gttcctaaga
    43741 ttatttttag agtgtttcaa tcattgactg tttttattac tgttcttctt ttgttgttcg
    43801 cagctctagg tatcggaata ggtgctggct actttgccta tcttgtcgaa gacacacaat
    43861 tgccgactaa aaaagaactg cagacagaac taggaaacat tacggaaacg tcaaagattg
    43921 tttatgcaga taatacagag atatcaaaaa tccagacaga tctgatgcgg acgactattt
    43981 catctgataa gatttcgcct ttattgaaaa ccgcgattat cagtacagaa gatgaatact
    44041 tcgataaaca tcaaggttat gtccctaaag ctgttttacg agcactagtt tcagaagcga
    44101 ctggcatcgg ctcgtctgga ggatccactt tgacacagca attagtcaaa caacagatcc
    44161 taaccgatga aacaacattc aaacgtaaag caaacgagat cgttctttct gcacaagtag
    44221 aaaaacactt ttcaaaaaat gagatcatta ctacgtattt gaatgtctca ccatttggtc
    44281 ggaataataa ggggcaaaat attgctggag tccaggaagc tgctagagga atctttggtg
    44341 tagatgcaaa ggatgtgact ctgccacaag ctgcttatat cgctggtttg cctcaaagcc
    44401 ctatcacgta tagtccatac acgaatacag gcgcgctaaa agaagatctt tctgccggtc
    44461 ttgccagaaa agattttgtt ttattcagta tgtaccgtga aggacaaatt acgaaagaac
    44521 aatacgaaga agcgaaagcc tatgatctta caaaagactt tctcccacaa caaatcgcgg
    44581 aacagactga cagagaattc ttgtattata ccgtgatgaa cgaagcaaca aggattattg
    44641 cacaacaatt ggcagaaaaa gataatgctg atatgtcaga ttcatcggtc agtgaagctt
    44701 attatcaaaa agcacagcaa acgatccaaa acaaaggata tacgatccat tcgacgatcg
    44761 ataaagatat ctatgctgca ttacaaaccg gcgtagaaaa ttatggctac atgttagatg
    44821 atggtgctgg tagccaagtg gagactggaa atgtattgat ggataacaga accggacgta
    44881 tctatggctt cgtgggtggt cggaattaca gccagaatca aaataaccac gcctttgata
    44941 cagaacgtca agctggttca tccatcaaac cggttcttgt ttacggtcct gcaatcgata
    45001 tgggacttgt cggatcagaa tcccgtgtat cagattatgc aacgacttgg caagaaggaa
    45061 aaaatgctgg tgagaaaatc gtcaatgcga ccaatgaagg gtcgaacacc ttccaaaccg
    45121 tccgtgagtc actggaatgg tccaacaata tcccagctta tcacttatat caagatgtgt
    45181 tgaattccgg tggatcgaaa caatacgctt acgagcacta cttggcgaag atgaactacc
    45241 cagcaaatga taattggggc gttgaatcgg ctcctttagg gacagtagat gtaacgacgc
    45301 ttcaacagac aaatggattc caggccttag caaatggtgg agtctacgaa gaaggctata
    45361 tcattgattc catcacagat aatgcaggta atgtgattta taaacatgaa agtaattctg
    45421 tccgtattta ttccgaagca acggcatcga tcatgaatga catgatgcgt agtgtgatca
    45481 atgaaaaaat cactacacca ttcaaagatg tcatctcaag cttaaacgga aatctaggaa
    45541 atgcagattg ggtcggaaaa actggttcta ccaatgaata ccgagattca tggctgatcg
    45601 tctcaactcc atctatcact atcagtagtt gggcaggaca cgatgacaat actggaatgg
    45661 acagccaagc aagaatacgt tcggctaatt atcttgccaa tctaatcaat caagcttacc
    45721 aagcaaaccc tgagattttc ggcactgatg agaaatttga cttatcatcc gatgtgatga
    45781 agaaaaaagt atctgggttc acaggacaaa cagctggaaa agttactgtc aataaaaaat
    45841 ccatccaaac gccaggaaaa acagtggagt cactttgggc aaaaaatggt ccgaagaaaa
    45901 gcgcctttaa attcggcgta ggcggtactg atgaaaacta tacagattac tggaacaccg
    45961 cttcagctta tgctagagcg aatccagaga ccaaagaatc agacgataaa gatgattgat
    46021 ctaatagatt aatcagaatt aaagggagct gcgacaaaaa tcttgtcaca gctccctttt
    46081 tccgaataaa tggtgttcaa aagcttacat ctacgatatt aagacaaatt tatgtaaaaa
    46141 tgtgaaaaac
//
LOCUS       DAFBQH010000012        45120 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.012, whole genome shotgun sequence.
ACCESSION   DAFBQH010000012 DAFBQH010000000
VERSION     DAFBQH010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.012"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            212..604
                     /locus_tag="LK694_000770"
     CDS             212..604
                     /locus_tag="LK694_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK5051660.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="LK694_000771"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="LK694_000771"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBK5051661.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="LK694_000772"
     CDS             3165..4775
                     /locus_tag="LK694_000772"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBK5051662.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="LK694_000773"
     CDS             5067..5936
                     /locus_tag="LK694_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBK5051663.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="LK694_000774"
     CDS             6101..7372
                     /locus_tag="LK694_000774"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBK5051664.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="LK694_000775"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="LK694_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBK5051665.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="LK694_000776"
     CDS             8763..9026
                     /locus_tag="LK694_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBK5051666.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="LK694_000777"
     CDS             9471..9920
                     /locus_tag="LK694_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5051667.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="LK694_000778"
     CDS             9977..11395
                     /locus_tag="LK694_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK5051668.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="LK694_000779"
     CDS             11395..12255
                     /locus_tag="LK694_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBK5051669.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="LK694_000780"
     CDS             12429..13202
                     /locus_tag="LK694_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK5051670.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="LK694_000781"
     CDS             13349..13714
                     /locus_tag="LK694_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBK5051671.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="LK694_000782"
     CDS             complement(13862..15070)
                     /locus_tag="LK694_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5051672.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="LK694_000783"
     CDS             complement(15089..15367)
                     /locus_tag="LK694_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051673.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="LK694_000784"
     CDS             15521..16216
                     /locus_tag="LK694_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBK5051674.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="LK694_000785"
     CDS             complement(16658..17455)
                     /locus_tag="LK694_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBK5051675.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="LK694_000786"
     CDS             complement(17452..18636)
                     /locus_tag="LK694_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBK5051676.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="LK694_000787"
     CDS             complement(18661..19908)
                     /locus_tag="LK694_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBK5051677.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="LK694_000788"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="LK694_000788"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBK5051678.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="LK694_000789"
     CDS             21888..22202
                     /locus_tag="LK694_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBK5051679.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="LK694_000790"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="LK694_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBK5051680.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="LK694_000791"
     CDS             22887..23531
                     /locus_tag="LK694_000791"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBK5051681.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="LK694_000792"
     CDS             23546..23875
                     /locus_tag="LK694_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBK5051682.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="LK694_000793"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="LK694_000793"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBK5051683.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="LK694_000794"
     CDS             24863..25687
                     /locus_tag="LK694_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBK5051684.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="LK694_000795"
     CDS             25680..26027
                     /locus_tag="LK694_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBK5051685.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="LK694_000796"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="LK694_000796"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBK5051686.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="LK694_000797"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="LK694_000797"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBK5051687.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="LK694_000798"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="LK694_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBK5051688.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="LK694_000799"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="LK694_000799"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBK5051689.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="LK694_000800"
     CDS             31657..32451
                     /locus_tag="LK694_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5051690.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="LK694_000801"
     CDS             32441..34483
                     /locus_tag="LK694_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5051691.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="LK694_000802"
     CDS             34764..34913
                     /locus_tag="LK694_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBK5051692.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="LK694_000803"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="LK694_000803"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBK5051693.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="LK694_000804"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="LK694_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBK5051694.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="LK694_000805"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="LK694_000805"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBK5051695.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="LK694_000806"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="LK694_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBK5051696.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="LK694_000807"
     CDS             39330..40412
                     /locus_tag="LK694_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBK5051697.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="LK694_000808"
     CDS             40412..41215
                     /locus_tag="LK694_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBK5051698.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="LK694_000809"
     CDS             41285..42025
                     /locus_tag="LK694_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBK5051699.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="LK694_000810"
     CDS             42036..43040
                     /locus_tag="LK694_000810"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBK5051700.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="LK694_000811"
     CDS             complement(43085..44929)
                     /locus_tag="LK694_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051701.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14533 a         7847 c         9375 g        13365 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    45001 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    45061 tttttacagc tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat
//
LOCUS       DAFBQH010000013        43872 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.013, whole genome shotgun sequence.
ACCESSION   DAFBQH010000013 DAFBQH010000000
VERSION     DAFBQH010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.013"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(6..1047)
                     /locus_tag="LK694_000812"
                     /pseudo
     CDS             complement(6..1047)
                     /locus_tag="LK694_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
     gene            complement(1324..3126)
                     /locus_tag="LK694_000813"
     CDS             complement(1324..3126)
                     /locus_tag="LK694_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5051702.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(3140..3892)
                     /locus_tag="LK694_000814"
     CDS             complement(3140..3892)
                     /locus_tag="LK694_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5051703.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(3901..4263)
                     /locus_tag="LK694_000815"
     CDS             complement(3901..4263)
                     /locus_tag="LK694_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK5051704.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(4439..5311)
                     /locus_tag="LK694_000816"
     CDS             complement(4439..5311)
                     /locus_tag="LK694_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBK5051705.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(5362..5850)
                     /locus_tag="LK694_000817"
     CDS             complement(5362..5850)
                     /locus_tag="LK694_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBK5051706.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(5988..6779)
                     /locus_tag="LK694_000818"
     CDS             complement(5988..6779)
                     /locus_tag="LK694_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBK5051707.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GKSYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(6834..7442)
                     /locus_tag="LK694_000819"
     CDS             complement(6834..7442)
                     /locus_tag="LK694_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBK5051708.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(7435..7752)
                     /locus_tag="LK694_000820"
     CDS             complement(7435..7752)
                     /locus_tag="LK694_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK5051709.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(7908..8636)
                     /locus_tag="LK694_000821"
     CDS             complement(7908..8636)
                     /locus_tag="LK694_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBK5051710.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            8795..9676
                     /locus_tag="LK694_000822"
     CDS             8795..9676
                     /locus_tag="LK694_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK5051711.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERANRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(9913..10956)
                     /locus_tag="LK694_000823"
     CDS             complement(9913..10956)
                     /locus_tag="LK694_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK5051712.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(10959..11627)
                     /locus_tag="LK694_000824"
     CDS             complement(10959..11627)
                     /locus_tag="LK694_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5051713.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            11769..14105
                     /locus_tag="LK694_000825"
     CDS             11769..14105
                     /locus_tag="LK694_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5051714.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(14220..14531)
                     /locus_tag="LK694_000826"
     CDS             complement(14220..14531)
                     /locus_tag="LK694_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051715.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            14712..16187
                     /locus_tag="LK694_000827"
     CDS             14712..16187
                     /locus_tag="LK694_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBK5051716.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            16234..16818
                     /locus_tag="LK694_000828"
     CDS             16234..16818
                     /locus_tag="LK694_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBK5051717.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            16820..17239
                     /locus_tag="LK694_000829"
     CDS             16820..17239
                     /locus_tag="LK694_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBK5051718.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(17520..17804)
                     /locus_tag="LK694_000830"
     CDS             complement(17520..17804)
                     /locus_tag="LK694_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBK5051719.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(18036..18746)
                     /locus_tag="LK694_000831"
     CDS             complement(18036..18746)
                     /locus_tag="LK694_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBK5051720.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(18743..20215)
                     /locus_tag="LK694_000832"
     CDS             complement(18743..20215)
                     /locus_tag="LK694_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBK5051721.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            20353..20826
                     /locus_tag="LK694_000833"
     CDS             20353..20826
                     /locus_tag="LK694_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK5051722.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(20979..21530)
                     /locus_tag="LK694_000834"
     CDS             complement(20979..21530)
                     /locus_tag="LK694_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK5051723.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            21635..22165
                     /locus_tag="LK694_000835"
     CDS             21635..22165
                     /locus_tag="LK694_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBK5051724.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(22648..23091)
                     /locus_tag="LK694_000836"
     CDS             complement(22648..23091)
                     /locus_tag="LK694_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5051725.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(23107..24267)
                     /locus_tag="LK694_000837"
     CDS             complement(23107..24267)
                     /locus_tag="LK694_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK5051726.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            24774..26474
                     /locus_tag="LK694_000838"
     CDS             24774..26474
                     /locus_tag="LK694_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051727.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(26576..27409)
                     /locus_tag="LK694_000839"
     CDS             complement(26576..27409)
                     /locus_tag="LK694_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBK5051728.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(27713..29878)
                     /locus_tag="LK694_000840"
     CDS             complement(27713..29878)
                     /locus_tag="LK694_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK5051729.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            30138..32432
                     /locus_tag="LK694_000841"
     CDS             30138..32432
                     /locus_tag="LK694_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBK5051730.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            32425..33090
                     /gene="pgmB"
                     /locus_tag="LK694_000842"
     CDS             32425..33090
                     /gene="pgmB"
                     /locus_tag="LK694_000842"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBK5051731.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            33083..34090
                     /locus_tag="LK694_000843"
     CDS             33083..34090
                     /locus_tag="LK694_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBK5051732.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            34112..35131
                     /locus_tag="LK694_000844"
     CDS             34112..35131
                     /locus_tag="LK694_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5051733.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(35412..36779)
                     /gene="nhaC"
                     /locus_tag="LK694_000845"
     CDS             complement(35412..36779)
                     /gene="nhaC"
                     /locus_tag="LK694_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBK5051734.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(36888..38309)
                     /locus_tag="LK694_000846"
     CDS             complement(36888..38309)
                     /locus_tag="LK694_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5051735.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(38552..40429)
                     /gene="tdc"
                     /locus_tag="LK694_000847"
     CDS             complement(38552..40429)
                     /gene="tdc"
                     /locus_tag="LK694_000847"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBK5051736.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(40720..41976)
                     /locus_tag="LK694_000848"
     CDS             complement(40720..41976)
                     /locus_tag="LK694_000848"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBK5051737.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(42088..42293)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            42556..43857
                     /locus_tag="LK694_000849"
     CDS             42556..43857
                     /locus_tag="LK694_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBK5051738.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
BASE COUNT        13392 a         9039 c         7784 g        13657 t
ORIGIN      
        1 ttattctatt tctttatatt tattttgtat tccgttacaa actcgcgtac ttggttaggt
       61 tccagagtga catctccaaa actttcaagt cgctccgctc ctggcagttc ttgagtctca
      121 aatgtgatac cgccatgatt tactaattta ttcccgcgca tttccggtcc atcttctcca
      181 aaatttgctg taaaaatcac gattcctgaa gcatctgttt ttacttggat cgatactttt
      241 tcatctgggc ttgtcaattc cgcagcattt agtaaaccag attgtttttt caacaaaaat
      301 ggatgatcga tgccgcccac tatcattgat tggcgaaaat cactggcaaa gacctcacct
      361 aattttttag gacgttggaa gtcaaatgct gtgccactta ctgaaactac ttctcctgta
      421 ggcgttgtat ctttattgag cgtagcaaat aatcggctat tcaaccaaag tatatgttcg
      481 tcgattgact gtgctggatc accggttaaa ttgaaataga catgatttgt aggattgaat
      541 actgttttgc ggtcactgat cccttggacc gtcactcgcc atgtatcatc gttcgtcagc
      601 gtataccgta cttctacatc taaagttcca ggaaatccgt tttccatatc tgggctagtc
      661 tgatagaaaa tgaccgatgc ttcgttttca ccattgatga ctgtataatg ccactgcttg
      721 gcttcaaagc taggtgcacc accatgtaaa ccaatgacca gtgtccgggt ctgtttttac
      781 ttcgaacgtt tcatcatcta atttgaatgt gccattcgta atccgtccag ccatacgtcc
      841 gattgaagca ccgatataag ggtctttttc tagatattct tttgcttcat caaaacctaa
      901 gatcaactct cgtttttcat tatcgattat tgtttgaaaa tgaacaaccc gtgccccaag
      961 atcactgaca gacaaagatg tcccattttg attcgtgatc gtaatcaagt gatagttttc
     1021 gccaaaattt tgttcattga tttccatgtt ccttgcaccc tcttttcttt catttgcact
     1081 gattccagca tacatggaaa ccgttttctt aacagcttat tccgtgagta acgagaaaaa
     1141 atttactata ttctaataaa agaccgtgaa aaaaaggttg tgacaaaaat attgtcacaa
     1201 cctctttttc cgaacaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa
     1261 acgaaaaata tgaggagcta tttttgcttg gctgttctta ttacttgatg caggacagct
     1321 ttttcacaat ctctttttca atacgattcc agtatataac catgtcgtca gtacatagta
     1381 gatgaaatac aatacgaaga atatcgtaaa cggaacccaa atatctttgt atggattttg
     1441 catgaataga ccaaacattt gtaatccgaa taagacatgg actacaccta agattcctgg
     1501 tgctaagaat aacacgccta cttctctacg gatcgattgt ttaagcaacc cgcgtcgtgt
     1561 tcctatcttt tcaagcattt cataacgagc aatgtcactc ttgcttcctg acaagatctt
     1621 gaacatcaaa cagctagcaa gcatggtcaa gaaagctaaa cctaggaaga atcccataaa
     1681 ttcaaaacca gagaatagac cattatacac ttcatacatt tcatatttct ggttgctgta
     1741 ctctactttt tgcaaagatg gattgttcat tttattttga gcaacaagtg tttcgattgg
     1801 tttcaggttt gcttggaagt cttttacctg aactagctgt aaagtggatt gtggcaagtt
     1861 cagttgatca aattctgatt gactgactac cttgatttct ttttcttgct gttccggcaa
     1921 ttcatagctt cgaagttcct cttgtacagt cagttcattc atttttttag caggaacttt
     1981 ttctttcgtg atcttcggcg gtgttccatc ttcatgtttt atcatgatca atggttgagc
     2041 cgcaaattca cttgcattat agtaaatcgt tcccgcatct tctttttgag tataagaagc
     2101 agaaagtgtt ggtgaaagtt ctttgatttt ttgctgatct tctgcccgag gattattcat
     2161 cactagatca tatccagaat tgttttctgt caagtctgca atttcatttt taaaacctaa
     2221 tcctaccgtc aaagcgccca gtgccaaggc aaacagcata gcgaccattg agagcatttg
     2281 tgtatattca cgaatacgaa agtttaactg tgacaatgtg aaattgttca attttttcaa
     2341 tgaaatctgt tctgatttct ttagtaatga taaaaagaaa atgatcacag aatggaaaat
     2401 gaaataagtt cctaatacga tcgtaactaa ggctacccct ataccaaaga cttgataggt
     2461 ccccacgtgt ttcatcatat agtaaccgca gcctagaaaa gcaattccca gtactacttc
     2521 aaggaaaaac aagaatttat gctgcttgat tcttgttggt gtctgatccg cacgaatcaa
     2581 tgctaaaatc ggttttttga caatcgaaaa agcattgaca acagcagcta ataaaaacaa
     2641 aacagcaaaa aacaaaacag tgatcaacaa cccgtgaata ctaaaaggtg taaaatgcga
     2701 aatattgaga tctaactgat ccactaataa tcggtggacg atacttgtca atcctacacc
     2761 gacagctgaa cccactaaag tagctgcgat ccctactaag aatgtttcag aaaaaatcat
     2821 ctgtgcgatt tttctgcctt ttgctcccag catcataaac attgcatagt ctttttgccg
     2881 catcgtcatc aaaaaggaat ttgcatataa aatataaacg aatgtgatga tccctagtaa
     2941 aacagaaccg aatcggaaaa tgataactgt agccgcaacg atcgtattac ttttcaaaaa
     3001 agcttcgttg cttgccattg attcaaacat gtaaaaaata gcagatgcca taactaatcc
     3061 tgagaataac accaagtagt ctcttaaacg gctcttgatc ccagtaagtg ataatttcca
     3121 aagcatagtc aatctccccc tactgttcca agtttccaag aatagatagt atttctcgat
     3181 agaaggaatc acggctttga tctccacgtt tcacttcttg atggataccg ccatccttga
     3241 tgaataaaat acgttggcaa taacttgcag aaaacggatc atgcgtcaca agaagaatcg
     3301 atacatgatc ttttgtattc aattcgtcca tcgtatctaa aagatcccgg gcacttttgg
     3361 aatccagcgc acctgtaggc tcatccccta atagaatcgt tggttgtgtg atcaatgcac
     3421 gtgcggcggc gacccgttgt ttttgtccac cggaaatctc agaaggatag ctttccaaaa
     3481 tatgagagat ggataaacgt tctgcaattt tttggacacg ttgcttgatt tcttttggtc
     3541 tgacgccttg taaagaaaga ggaacagcga tattttcttg tgctgtcaag ctttctaata
     3601 aattgaaatc ttggaaaata aagccaatct cattcgcacg aaaatctgct agttgatttc
     3661 cttttaatgt ggtcacatct ttttgattga ttcggatatg cccatctgtt ggtttatcta
     3721 atgtggataa aatattcaaa agtgtcgact taccagaacc agaagcgccc atgatcccaa
     3781 taaattcgcc tttttctaca tcgaagctga tgccgcttaa tgcttgcgtc tttttctcat
     3841 tgttttttcc gtatactttt gttacattct gtacttcgac tattttgttc atttctttcc
     3901 ctacccttta attagttttt ccagtgctgt tttcggcacg tctttttttt cgacttcttc
     3961 atacgaagtg acacctttat ttttgttcca ggtgattttt aaatatgctc ctttacgaag
     4021 cggtcgagtc aattgtccct tgaagtccag ttctttttcc ttaccctctt tcgtatagcc
     4081 aggcaggata tatctatagt tgactgtttc ttgtccagag tctgattttg aaactgtttt
     4141 tgtaccatct gtcgtaattt gtacatagta ttcttctcca cccataacta cgtaatctgc
     4201 gattttcacc cctgcaagta ccacaacgat gaaaacaagt agaccgatca gaagtttttt
     4261 cactttttcc ccttctctct tactttatat actgattata gaaaaaagcg aacaattctg
     4321 ccttcgaatc aactatcatt cttctttcaa ttttgtcatt ttctttaaaa caactaaaaa
     4381 aagagttcaa gacaataatc cgtcctaaac tccttcttcc tatcttacaa aaagtatgtc
     4441 acttacggtc tttcatgtcg cctgtattga taacaggttg cgttcctttg tctgtaatga
     4501 ttgagacaag taaattattg atcaattgga ctttccgatc atcggtgaaa ttgatttctt
     4561 gtccatcttc gatttgctct aaggccattt gagtgattgt gacagctcct tcaacaatgg
     4621 tttgtcgagc agacaaaatg gcttttgctt gttgacgttg gagcatggca ctagcgattt
     4681 ctgttgcata ggctaaatgg ttcaagcgtg tttcgattac ttcgacacct gctactgcta
     4741 aacgctcttg cagctctttt tgcaactcgt cagacactgc cgtcgtgttt cctcgaagcg
     4801 tcaaatcatt ctcattgaat gtatcatacg ggtattggct agcgatatgc cgaatggctg
     4861 tctcactttg gatttctaca aaatcttgat aatacgctac atcgaataat gcctttgctg
     4921 tatcaacgac tttaaagact actactgctg agatttcgat ggggtttcca tccaaatcat
     4981 tgacttttaa aacagaacta ttgaaattcc gaactttcaa cgaaacagtc attttttgtg
     5041 ccaaaggtat cgtaaggaaa aagccatttt ctcgaatcgt acctaaatat tgtccaaaaa
     5101 acaaaattac ttttgcttga ttaggactga ctacagttgc cgaacttaac aacaataacg
     5161 tgatcaacca aagaaaaata cttaaaaaaa gtgcccaaac tttcgcttgc cacataccta
     5221 aatagaagaa aaacaatcca actaaagcta atacgaccaa tccgacaatc cctgcataac
     5281 cattgatata aaatgctctt ttttctctca tgctacttcc tgcgctcctt tatccttcaa
     5341 taaatatcta atgttacttt tttaattttt ttttcgaata cgcgccatta caccttctag
     5401 ttctaactct ttgtcttcag tgatgtacca accgcttgcc tgtgtccctc cggctgcctg
     5461 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
     5521 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
     5581 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
     5641 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
     5701 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
     5761 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
     5821 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
     5881 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
     5941 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
     6001 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
     6061 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
     6121 ttaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
     6181 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
     6241 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
     6301 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
     6361 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
     6421 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
     6481 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
     6541 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
     6601 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
     6661 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
     6721 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
     6781 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
     6841 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
     6901 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
     6961 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
     7021 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
     7081 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
     7141 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
     7201 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
     7261 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
     7321 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
     7381 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
     7441 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
     7501 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
     7561 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
     7621 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
     7681 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
     7741 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
     7801 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
     7861 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
     7921 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
     7981 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
     8041 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
     8101 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
     8161 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
     8221 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
     8281 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
     8341 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
     8401 atcccattac ctaaatgaac aatcaaacca atcgctgttc ctatcactaa agcaaaaata
     8461 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
     8521 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
     8581 ccaattagtc cgccaaacaa aacggaacct acattgatca aaactccagt tggcatcaaa
     8641 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
     8701 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
     8761 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
     8821 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
     8881 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
     8941 acgcgccaat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
     9001 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
     9061 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
     9121 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
     9181 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaag
     9241 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
     9301 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
     9361 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
     9421 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
     9481 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
     9541 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
     9601 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
     9661 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
     9721 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
     9781 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
     9841 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
     9901 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
     9961 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    10021 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    10081 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagta caatctctgt
    10141 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    10201 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    10261 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgaa aaaaaacgat
    10321 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    10381 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    10441 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    10501 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    10561 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    10621 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    10681 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    10741 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    10801 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    10861 aataaaaccg agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    10921 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    10981 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    11041 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    11101 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    11161 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    11221 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    11281 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    11341 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    11401 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    11461 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    11521 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    11581 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    11641 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    11701 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    11761 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    11821 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    11881 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    11941 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    12001 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    12061 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    12121 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacaaatcat atgcataaaa
    12181 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    12241 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    12301 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    12361 ataatcctga actggcccat caatacgcag atcgaatcat tgaattttct gatggtaaga
    12421 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    12481 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    12541 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    12601 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    12661 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    12721 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    12781 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    12841 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    12901 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    12961 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    13021 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    13081 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    13141 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    13201 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    13261 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    13321 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    13381 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    13441 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    13501 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    13561 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    13621 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    13681 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    13741 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    13801 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    13861 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    13921 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    13981 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    14041 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    14101 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    14161 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    14221 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    14281 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    14341 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    14401 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    14461 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactcctcc
    14521 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    14581 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    14641 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    14701 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    14761 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    14821 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccaa
    14881 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    14941 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    15001 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    15061 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    15121 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    15181 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    15241 atacgatcgg tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat
    15301 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    15361 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    15421 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    15481 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    15541 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    15601 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    15661 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    15721 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    15781 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    15841 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    15901 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    15961 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    16021 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    16081 taagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    16141 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    16201 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    16261 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    16321 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    16381 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    16441 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    16501 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    16561 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    16621 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    16681 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    16741 gttaatcacc atacaatgaa acttttggat caatttgaaa ttgtaaatga gcattcaaaa
    16801 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    16861 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    16921 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    16981 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    17041 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    17101 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    17161 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    17221 tggtgaaaaa ccattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    17281 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    17341 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    17401 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    17461 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    17521 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    17581 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    17641 ttcacgatac tgccttcata tggcacattg tcaaaagttg cttggacttt aactcttcca
    17701 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgccgact
    17761 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    17821 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    17881 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    17941 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    18001 gctcacaacc tcttccttaa acggtgttca aaatattatt cgccttttgc ttctttcact
    18061 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    18121 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    18181 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    18241 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    18301 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    18361 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    18421 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    18481 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    18541 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    18601 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    18661 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    18721 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    18781 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    18841 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    18901 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    18961 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    19021 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    19081 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    19141 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    19201 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    19261 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    19321 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    19381 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    19441 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    19501 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    19561 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    19621 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    19681 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    19741 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    19801 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacac
    19861 cgcaagcttg gatcaaacga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    19921 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    19981 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    20041 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    20101 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    20161 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    20221 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    20281 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    20341 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    20401 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    20461 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    20521 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    20581 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    20641 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    20701 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    20761 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    20821 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    20881 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    20941 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    21001 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    21061 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    21121 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    21181 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    21241 ttaaccggta gttcattggt gggtgtatga atccactctg ccaattgttc cttcccttct
    21301 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    21361 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    21421 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    21481 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    21541 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    21601 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    21661 ttttattgga actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    21721 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    21781 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    21841 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    21901 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    21961 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    22021 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    22081 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    22141 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    22201 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    22261 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    22321 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    22381 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    22441 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    22501 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    22561 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    22621 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    22681 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    22741 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    22801 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    22861 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    22921 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    22981 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    23041 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    23101 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    23161 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    23221 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    23281 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    23341 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    23401 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    23461 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    23521 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    23581 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    23641 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    23701 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    23761 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    23821 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    23881 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    23941 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    24001 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    24061 gtttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    24121 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    24181 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    24241 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    24301 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    24361 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    24421 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    24481 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aatagcttct catattttct
    24541 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    24601 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    24661 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    24721 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    24781 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    24841 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    24901 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    24961 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    25021 ccgtttacgg cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    25081 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    25141 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    25201 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    25261 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    25321 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    25381 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    25441 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    25501 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    25561 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    25621 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    25681 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    25741 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    25801 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    25861 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    25921 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    25981 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    26041 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    26101 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    26161 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    26221 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    26281 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagcaaa
    26341 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    26401 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    26461 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    26521 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    26581 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    26641 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    26701 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    26761 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    26821 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    26881 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    26941 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    27001 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgaaattgg gagacggtaa
    27061 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    27121 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    27181 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt accggacaga
    27241 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    27301 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    27361 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    27421 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    27481 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    27541 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    27601 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    27661 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    27721 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    27781 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    27841 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    27901 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    27961 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    28021 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    28081 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    28141 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    28201 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    28261 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    28321 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    28381 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    28441 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    28501 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    28561 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    28621 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    28681 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    28741 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    28801 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    28861 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    28921 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    28981 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    29041 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    29101 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    29161 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    29221 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    29281 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    29341 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    29401 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    29461 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    29521 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    29581 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    29641 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    29701 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    29761 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    29821 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    29881 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    29941 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    30001 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    30061 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    30121 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    30181 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    30241 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    30301 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    30361 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    30421 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    30481 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    30541 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    30601 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    30661 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    30721 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    30781 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    30841 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    30901 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    30961 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    31021 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    31081 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    31141 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    31201 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    31261 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    31321 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    31381 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    31441 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    31501 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    31561 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    31621 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    31681 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    31741 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    31801 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    31861 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    31921 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    31981 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    32041 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    32101 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    32161 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    32221 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    32281 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    32341 gttctcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    32401 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    32461 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    32521 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    32581 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    32641 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    32701 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    32761 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    32821 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    32881 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    32941 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    33001 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    33061 ttgaaagaaa agtggcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    33121 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    33181 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    33241 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    33301 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    33361 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    33421 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    33481 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    33541 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    33601 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    33661 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    33721 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    33781 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    33841 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    33901 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    33961 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    34021 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    34081 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    34141 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    34201 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    34261 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    34321 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    34381 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    34441 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    34501 ttcagatagt caagatactg tcattgatta tttataccaa aaccacatcc ctttcacttt
    34561 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    34621 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    34681 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    34741 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    34801 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    34861 cttcgccctt cgcacgatgc agttggcgca aatgtatggc tatgtcattc cagagacact
    34921 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    34981 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    35041 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    35101 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    35161 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    35221 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    35281 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    35341 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    35401 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    35461 aagacaggtg caagaatcga gaaaaaggcg aatggcaagt aatgcaacgc gctaactttc
    35521 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    35581 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    35641 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    35701 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    35761 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    35821 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    35881 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    35941 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    36001 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    36061 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    36121 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    36181 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    36241 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    36301 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    36361 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    36421 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    36481 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    36541 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    36601 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    36661 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    36721 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    36781 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    36841 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tattttttta tttttcttct
    36901 aaactttttt ctacctcaat cccccaagaa tctttcttga agtgattgat gatcaatgga
    36961 ataacgaaca tgaccactgt gatagctgct actaataatg catcactcaa tgtcgctgta
    37021 cctaatgtag caaatacgac aacgatgata gagaagatca gcatgcagct gactacccaa
    37081 gctaatccgt ttccgttttt accaattcgg taaggacgtt cgaattctgg atgtttctta
    37141 cgcattttaa gcaatgaaac agcaatcaaa atgtacacga tacaataaat aacagttgta
    37201 gcatttgtaa gtgtcaagaa tacagcactt acgtcatcca ttacaccata aagcaaagca
    37261 aataatgaaa taacgattga ttgtgttaga acgacatttc tagatacgcc gtatttgttt
    37321 tctttatgga atccgaattt tggtggaagg aatccttcac gagctacttg gatgattgtt
    37381 tttgatggtc cagtaaccca agctgataat tgtaaaagca caccaataaa taccatgaag
    37441 ctgaagatat ttccgatcac ttctggtaag cctaggattt gacagtcaat caagataggc
    37501 tgcgtgatat ttgctaattc catttttcca tctggtacga catttgctac aagcatcgca
    37561 ttgatgacat tcaataaaac taatccgatc aaagcgataa atacgccttt tgtataattt
    37621 tttgttgcgt ctttcaaacg aggcatgtaa acagaactca tttcgatccc aacaaaaata
    37681 aatgtgatac cagctaagta tttcaatgaa tcaatatgtt ccaagtcagg gaaagctttt
    37741 gaccatgaaa agtcacctag atagccgttt gagactagtc cgactttgaa ggctgccaaa
    37801 acacctaaaa cgaacatgat gactactggg atatataccc ctagccatac accgatgttc
    37861 ccgccgactt ttgccatatc gaatttcaag ttcaagatcg tgatgatcca gtagataact
    37921 aaaatacaac ctaaaacgaa ccagtgatta ttgccaagtt ctacattacc aaatgtgttt
    37981 cctaacaatg gtcctaaagt agaagcgacc attaccatac ctgggaacat ttgcacccat
    38041 aatagccatg caacgacaaa tccccatttg ctgcccagtg ctgtttttac ccataactga
    38101 ggaccgcctt cttgaggaag catcgtggaa agctcaccgg aaaccattga tatcggaccg
    38161 gcaaaaaaca aaactgcaaa aattgaataa aaaatcaatg tccaaccgac tgcggctaac
    38221 gtaggaatac tccgaaccgt tgcacacagt gccattgtca gaccgataaa ggtcccaaga
    38281 cccattttac ttacttcaga tttactcacc gttgacactc tccttgattt tttggataaa
    38341 ataacggtgt ctttgaagta gtttcagcaa taaacgaaac aattcagcga atatttgagg
    38401 ggattctttg agtttattgc ttgaagggtt tcttcaaaga caacctcaaa acaaagggat
    38461 cgaagagctt aggtctcccc ttgctcttca gatccaaaca tgcgtcagaa acagttagta
    38521 ttcaagctgt tttctaacat gattaccttg cttattttgc ttcgcttgcc aataattggt
    38581 ctgcgtagat tttttccaat ttttcttgaa gagctgcttt gattttttca gcatattctt
    38641 caaagtgttc ttctttgttc atgtatggtg tcattacgct ggcacgcaat acagttactt
    38701 ttccggcacg gttccattct tcatctgaga atcctagttg gttcacaaat tgtaatgggc
    38761 tgttgccata gtctgggata gcaaagtcag tatgtgaagt taagaactca ttaccgtaaa
    38821 tgcttccttt cacgtatgaa gaataatcat atacgtcatg gttcaattta ttcattgcaa
    38881 ctaagtcatc gttgcctttt tctttgaata catagtctac catattgaaa tctggataag
    38941 ttaatggatg aacttcgatt tctttgtctc ctactttaaa tgacaagtca ttcaagaagt
    39001 tgtagaaacg atgtgcacct tcgattgatg cacccattaa tttaccatat cctgtgacat
    39061 tcaaaggtaa gacatggtgt gctgcccaaa cgctggctgc tgttgcccct gcttttgaac
    39121 cttccaagat ataagcccca agtaatgctg ggatatctgc gcctttttca aatacatatg
    39181 tcgcaaagta agagataacg tctctcatgc ggatatcttt gatgacgata ccaccggcag
    39241 aatatggaac atagcccatt ttatgtgggt caatcgttac agattctgct tcttcgattg
    39301 ctttatatgc actgtgtact tcttctaaga tataatcttt gttttctgta aagacattgt
    39361 atttgtaatg aacatctttt aaatcttcaa atggaatgaa gttgttgtct tcgtctaaga
    39421 aaatcgcacg gccgtatccg ccataagcag catctacgtg taagtagaag taaataccgt
    39481 ctttttctaa tacgcgacgc aatgcaacga ttttatcgat accgtcgatt gctccttctt
    39541 ctgttgatcc tacaacaccg actacaccta ggattggtgt tttttcagct gctagaccac
    39601 gaacgatttt ttcaagttcg ttgatgtcca tgcgatagtt gtggtcaact ggcactggaa
    39661 tcacttggtc taaaccgata ccgatgatgt cggcagcttt tagccatgaa tagtgtttag
    39721 tttgtggaac caaccatttt cctaattttt ctaaatgttt tccgctacgt gctgagtgag
    39781 ctttgatttc gtcgattttt tctgggacgc tgtctaacag attcatgatt tcttctgttg
    39841 acaagttcat taattcccaa tcgctttttc cagcaactag ttcaggtgtc acttctttca
    39901 ttgcaagcgg caatgatttg atgttgcggg cataccaaag tccttctaag tttgctaaag
    39961 aaccatcagc aacgatgtgg ccccagccat ctttatagct cattaatttt gcaaattcca
    40021 ttcctacttc ttcttccatt tggcttgttg caggagaaga ttcataggca acgttattac
    40081 cattccatag catagcaaaa ttgtacgcta gtaaagatgg catcaatgtt tcagagttca
    40141 tatgacccca gtaacgtccg gcattatgcc aaggaacgga atgggtacgc atccgcgctg
    40201 aaatttctga tagaacatcc tttgttttat tcacagtgtg ttcaaaacta gcactgcttt
    40261 tttcttctgg cgtaataata ggcatgtctt gaggcatgta gttttgacgc cagcctaaat
    40321 gttcatccac caattcattc aacaaagctt tatagatttg gccgttctca gctttgtccc
    40381 caatgaatag ggcatttagg tttagatctt tcgacaatga ttcactcata gtgaaaccct
    40441 cctaaaaaaa tatttaaatt ttgtcgagcc ctacattaca cctgttcttc tattctgtaa
    40501 actaagtttt ggcctgattt ttaatcatat tttaatatgt atcaattatc cgaattgttc
    40561 acacttttga aataattaaa aaaaacgagg gaatttacaa atgtaaactc cctcgcaaag
    40621 attgtattaa cgacaaccag cgttctattt taggatgggt ttcaaaattg gaatgttgta
    40681 atagtatttc cctttttcaa ggtaaaaact tactaaattc taatcaatta ctttggccaa
    40741 aaagtaattt ttcttacctt ttcgaataac cacaaactta ccatcgaatt catcggatgg
    40801 atttatgaca aaatttaaat cggtaacgcg gtcaccgtta atacttatag ctccgtttga
    40861 aacatcttca cgtgcttgac gtttagatgg ttcaattttt gtggaaacaa gcagttccac
    40921 gatattttca ggtgtgcttg aaatttcaac gttcggcatt ttaccgaatc cttgcgcgat
    40981 ttcttccgca tttagttctt taatatttcc agagaacaat gcttgcgtta ttttttgcgc
    41041 ttctttcaag tcttcttcac tatgcacaaa tcttgtaact tcttctgcca aacggcgttg
    41101 tgcttcacgt ttctcaggtt ctgtctctac ttttttcgct aagtcttcga tttcttcttg
    41161 agacaagaaa gtaaagaatt tcaagtattt cactacatcg cgatcatctt gattcaacca
    41221 gaattggtag aactcaaatg gtgaagtttt cttcggatca agccaaacgg caccgcctgc
    41281 agtttttccg aatttcgttc cgtcagcctt cagcatcaat gggatcgtta aaccgaacgc
    41341 ttctgcttct ggtccttcta attttcggat taggtctaat ccagctgtga tatttcccca
    41401 ttggtctgct ccaccgattt gcaattgcac atcgtatgtt ttatgcagat gcaaaaagtc
    41461 gatagattgc aggatttggt aagtaaactc tgtaaatgag attcctactt ccaaacggct
    41521 ggcaacgata tcttttgcta acatcgtgtt gatgttgaag tttttaccgt agtctctcaa
    41581 aaattcaagt aaagagattt ttgacaacca gtcgtagttg ttcacaaatg tgatatttgc
    41641 atctttacca aacaattttc tcatttgatt ggaaagtgcg tccacgttat gttgcacttg
    41701 ttccatcgtt tgcaacacac gttctgtttt gcggccgctt gggtcgccga tcgttcctgt
    41761 tccgccgcca atcagaatat atggatgatg gcctgccaat tggaatcttt tcatcatcat
    41821 aaatggaatc aaatgtccga tatgcatcga atcaccagtt ggatcaacgc cgcaataaag
    41881 tgaaatgctc ttttcttcaa cgagctcgcg aagtctttct tcgtttgttt gctgattaat
    41941 ggcgtcgcgc caagttaatt catcaataat attcatttta ctgacctccg tgtagttttt
    42001 ttatgaaagc aggaacctgc tagcatgtgt aattaattat ttcttcagga taggatagga
    42061 aataaaaaaa gtcccttggc atataagcca agggacgaaa tctcgcggta ccacccaatt
    42121 tgtatgtcta gatatccaac atacctctcg aacatttaac gctgtcgcgt ggcaatcact
    42181 gccaaagact cctgaaatgt aattcgcttt aaagcatgta ctggtttgca gcgaccacca
    42241 gctttctgaa acattggctt taacaactac ttcgtttcga tcttcgtctt gttattatta
    42301 atatgtgtct atcatagagc gcaacttttt ctttgtcaac agaaaaagga tatttttttt
    42361 aaattcctca ttttttcttt tttgaatccg ctctaattcc gaacatttta ttttcaatta
    42421 ttgttaaaag aaataacttt ttctgtattt tttctgcaat ttatcgagaa gtcactttac
    42481 tttgcctctt attctttctt cgttacaata aggaatggga caagtaaaaa aatgaaaaag
    42541 caagggacga ggtatatgga aaaaaagcaa aaaattattt taggagccac ttctgcttta
    42601 actatcgtta ttttaattct aagtttgttt tttgtcatcc gatcatctac aaaaaaagaa
    42661 ccaatcattc ctgaaaaaaa aataacagaa aaagcaaaag aaccgacaga tgataagcaa
    42721 caactagtta ctcaagcaaa aaatctggct caaagctacg attatgacca agcgattgcg
    42781 ttattggaaa aagatagtaa gaacggatct actgaaacac aacagcttct tcaaacacta
    42841 aaaaaggaga aagaacaact gatcgactgg tctgatccta ctcagatctc tcatgtcttt
    42901 ttccacagct tgatcgtgga cccgcaaaaa gccttccact ctcaacaggc acaaggatac
    42961 aaagattata tggtgactgt tgaagaattc aaccgttcga tcgaacaact ttatcaaaat
    43021 gattttgttt tagttaattt gaatgatttg atccaaaaag acgaacaagg aaatctttcc
    43081 tttaacgggc ttgcgctacc tgaaggaaaa aaaccattgg ttctttcaca agatgatgtc
    43141 agctattatg aatatatgga taattccggt tttcctagcc gattggtcgt atcaaataac
    43201 aatgaaatca aaaatgtcta taaagagaaa gataaagaag aaatcggtga ctttgacatg
    43261 gttcctctga tcgatgcctt tgtcaagaaa catcctgatt tttcttatcg aggcgcaaaa
    43321 ggtaccttgg ccctgacagg ttacaacgga gttttaggtt atcgaacatc aaaatctcaa
    43381 tatggagaca atgaaaaaac aaaaaaagag attaaagaag ctaaaaaagt ggcggaacag
    43441 ttgaagaaag acggctggag ctttgcttcc catacatggg gtcacttgaa tatgactcaa
    43501 gcttcactag aagatgtaaa aaaagacaat gaattgtggc agcaagaagt tgcacctatc
    43561 ctaggaaaaa caaatattct gatttatcca tttggtgcag acatcagcga ctggcagcct
    43621 tattcagcag aaaatcaaaa atttgcttac ttgaagagtc aaggtttcga tattttctgt
    43681 aatgtcgatg cttctacacc agcttgggga caattaggta cggatttcta tcgaaatgct
    43741 cggatcaata tcgatggcat tcgttttcaa tctgatttag acggaaaaaa tcctattctt
    43801 gatccattca tcaatgtcca tgatgtctat gacaaagaag cacgcgaatc tgcttgattt
    43861 tacgtcattt at
//
LOCUS       DAFBQH010000014        43210 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.014, whole genome shotgun sequence.
ACCESSION   DAFBQH010000014 DAFBQH010000000
VERSION     DAFBQH010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.014"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            203..3481
                     /locus_tag="LK694_000850"
     CDS             203..3481
                     /locus_tag="LK694_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBK5051739.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(4022..5929)
                     /locus_tag="LK694_000851"
     CDS             complement(4022..5929)
                     /locus_tag="LK694_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBK5051740.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            6059..6934
                     /locus_tag="LK694_000852"
     CDS             6059..6934
                     /locus_tag="LK694_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBK5051741.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSEKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            7014..7988
                     /locus_tag="LK694_000853"
     CDS             7014..7988
                     /locus_tag="LK694_000853"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBK5051742.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8168..9313
                     /locus_tag="LK694_000854"
     CDS             8168..9313
                     /locus_tag="LK694_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBK5051743.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9450..9794
                     /locus_tag="LK694_000855"
     CDS             9450..9794
                     /locus_tag="LK694_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBK5051744.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            10065..11351
                     /gene="efmA"
                     /locus_tag="LK694_000856"
     CDS             10065..11351
                     /gene="efmA"
                     /locus_tag="LK694_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HBK5051745.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11489..12088)
                     /locus_tag="LK694_000857"
     CDS             complement(11489..12088)
                     /locus_tag="LK694_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HBK5051746.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12492..13613
                     /gene="mnmA"
                     /locus_tag="LK694_000858"
     CDS             12492..13613
                     /gene="mnmA"
                     /locus_tag="LK694_000858"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBK5051747.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNDKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13859..15280
                     /gene="arcD"
                     /locus_tag="LK694_000859"
     CDS             13859..15280
                     /gene="arcD"
                     /locus_tag="LK694_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBK5051748.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15294..16412
                     /locus_tag="LK694_000860"
     CDS             15294..16412
                     /locus_tag="LK694_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBK5051749.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16472..17764)
                     /locus_tag="LK694_000861"
     CDS             complement(16472..17764)
                     /locus_tag="LK694_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBK5051750.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17896..18474
                     /locus_tag="LK694_000862"
     CDS             17896..18474
                     /locus_tag="LK694_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK5051751.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18603..18980
                     /locus_tag="LK694_000863"
     CDS             18603..18980
                     /locus_tag="LK694_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBK5051752.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18913..20985
                     /locus_tag="LK694_000864"
     CDS             18913..20985
                     /locus_tag="LK694_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBK5051753.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21196..22143)
                     /locus_tag="LK694_000865"
     CDS             complement(21196..22143)
                     /locus_tag="LK694_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK5051754.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22389..22853
                     /locus_tag="LK694_000866"
     CDS             22389..22853
                     /locus_tag="LK694_000866"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBK5051755.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22915..23958)
                     /locus_tag="LK694_000867"
     CDS             complement(22915..23958)
                     /locus_tag="LK694_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBK5051756.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24335..25300)
                     /locus_tag="LK694_000868"
     CDS             complement(24335..25300)
                     /locus_tag="LK694_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBK5051757.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25534..26379
                     /locus_tag="LK694_000869"
     CDS             25534..26379
                     /locus_tag="LK694_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK5051758.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26570..27757
                     /locus_tag="LK694_000870"
     CDS             26570..27757
                     /locus_tag="LK694_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBK5051759.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPVRS
                     SRR"
     gene            27763..28119
                     /locus_tag="LK694_000871"
     CDS             27763..28119
                     /locus_tag="LK694_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBK5051760.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            28121..28459
                     /locus_tag="LK694_000872"
     CDS             28121..28459
                     /locus_tag="LK694_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBK5051761.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28535..28791
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28854..29888
                     /locus_tag="LK694_000873"
     CDS             28854..29888
                     /locus_tag="LK694_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5051762.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29881..30567
                     /locus_tag="LK694_000874"
     CDS             29881..30567
                     /locus_tag="LK694_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5051763.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30591..31439
                     /locus_tag="LK694_000875"
     CDS             30591..31439
                     /locus_tag="LK694_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBK5051764.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31636..32409
                     /gene="sufC"
                     /locus_tag="LK694_000876"
     CDS             31636..32409
                     /gene="sufC"
                     /locus_tag="LK694_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBK5051765.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32422..33708
                     /gene="sufD"
                     /locus_tag="LK694_000877"
     CDS             32422..33708
                     /gene="sufD"
                     /locus_tag="LK694_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBK5051766.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33705..34940
                     /locus_tag="LK694_000878"
     CDS             33705..34940
                     /locus_tag="LK694_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBK5051767.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34927..35397
                     /locus_tag="LK694_000879"
     CDS             34927..35397
                     /locus_tag="LK694_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBK5051768.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35402..36793
                     /gene="sufB"
                     /locus_tag="LK694_000880"
     CDS             35402..36793
                     /gene="sufB"
                     /locus_tag="LK694_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBK5051769.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(36888..38030)
                     /locus_tag="LK694_000881"
     CDS             complement(36888..38030)
                     /locus_tag="LK694_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK5051770.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(38069..38803)
                     /locus_tag="LK694_000882"
     CDS             complement(38069..38803)
                     /locus_tag="LK694_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5051771.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            39035..39310
                     /locus_tag="LK694_000883"
     CDS             39035..39310
                     /locus_tag="LK694_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5051772.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            39408..41405
                     /locus_tag="LK694_000884"
     CDS             39408..41405
                     /locus_tag="LK694_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051773.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(41481..41816)
                     /locus_tag="LK694_000885"
     CDS             complement(41481..41816)
                     /locus_tag="LK694_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5051774.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            41943..42158
                     /locus_tag="LK694_000886"
     CDS             41943..42158
                     /locus_tag="LK694_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5051775.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            42195..42557
                     /locus_tag="LK694_000887"
     CDS             42195..42557
                     /locus_tag="LK694_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBK5051776.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            42603..42929
                     /locus_tag="LK694_000888"
     CDS             42603..42929
                     /locus_tag="LK694_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051777.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT        14176 a         7702 c         9035 g        12297 t
ORIGIN      
        1 tgttttatgc tcttttttat acgttatttt aaaaaaactt attcttttta cgttaataaa
       61 tctctttatt atcaacaact taagttatat tttttctttt tataaaatac acttattata
      121 attttgtttt ttattgacag taaaaataaa ttaggtaata ctttaagtaa acgctttcta
      181 atttgttacg ggaggaatac acttgttaaa aaaacgtata aaatacagtg cgctaatgat
      241 gaatctcctt atgctcagta ccccggtttt aatttctgtc gttgaagttg ctgctaacga
      301 acaacagtct gtgaacgaag aaaatgcaca cgtgaacatt gaagggatgt caacaatgga
      361 ggaaatacca agaactagtg aagatatgga tacggaagaa ggaaatgatc ctatgatgaa
      421 agaatcagaa aatgaaaaga caaaaaagca atctgaagaa gcaaatgaag agtggaaagg
      481 ggttgttttc ggagaaagca caagtgctag taggtatgcc attgaaccat tagaagacgg
      541 cgtgcggctc tcttccacga ataacggcgg aaaattccag tcaagcggat cagatggaat
      601 gacctattat tatcaagcaa tccccaaaga catcaacttt aggatgcggg cgacgatcga
      661 aatagaatct tggacctata cgaatgccca agaaggattt gctttaatgg taagagacgc
      721 agtaccaaaa aatcatttgt ttgggcaggc attttattca aattcatttg ccatactagg
      781 cagtcggatc gaatatgtct gggactcgga tagacaagaa gtggtcaaca cgagcggcag
      841 caaatatacg atgcgacttg gattggggac acgggcaatc acaggaatat catctgaaga
      901 tccgcaagct gcgccagctc ctgggagtgt ctcagtggaa acaacacctt tagagacttc
      961 agcagggttc ttggaaaaag aaacaggttc ttataatata ttcggaaacg gtcatgggaa
     1021 tctgtttccg gccacgaact cgattactaa actggacgta gagatgaaaa aaacgaatac
     1081 tggtttagag gcatattatt acgatcctga aacaggagaa gaaatggctt ctgacatcat
     1141 gtatgactgg gaaaaattat acgcttcaga tgaatcggtt atttatgcag gatttgctgc
     1201 agcaaggaat atgacgatca aggtagaagg aatcgaagtt gcttactcag atcctgcgac
     1261 agatcctcct gggcaagaaa gacctactcg cttgattgat ccgatttaca ctgtgacctc
     1321 tagtaatcat tcaggaaacc aagatcatac tatgtccttt agagcaaatg ctgacggatt
     1381 attgacaatt aaaaataaag caacaggaga ggtgcttaag aacgcaaaaa acttggctat
     1441 cactgcgaat cgggaatttg attatcagac aaaactgatg gaaggaacga acgaatttac
     1501 tttcagtttt acgccagata aaaactatcc gccagaagaa tacgtagaga tgactagtta
     1561 tgaaacaaaa gagattcttc atacagtgga ctaccgtaaa cctaaatatt cgacagttta
     1621 tgtaacacca gaagggtcag atgcaggaaa cggaagcaga caaaatccat tatctctgac
     1681 acaagcattg agatacgcat atgctgggca gacaatcgtc atggcgcctg gaacttattc
     1741 cttcgaaaga ggattgacga ttccaaaagg cgttaacggg acaagagaaa acccgattca
     1801 gttgattgca gagaaaaatc cagaaaacaa gcgtcctgta cttgatttta aaggaactgg
     1861 aaatgggatg acactgttca gtcactactg gggtttacga gggtttgaca ttacaaatag
     1921 tgcagctatg caaaaaggat tacagatttc tggaaatcat aatttgatcg aagagattca
     1981 tgcttatcgg aatggaaata caggaatcca aatcagcggt agctcaaatg atcctttcga
     2041 agcatggccc gcgcataact tagtcaagaa ctgtacatct tttgaaaatg ccgatccagg
     2101 atttgaagat gccgatggat ttgcggccaa actgacaata ggagaaggaa atgtatttga
     2161 cggttgtatc gcgtatcata atgcagatga cggttgggac ctttttgcta aaaatgaaac
     2221 aggttcgatc ggaaaagtaa tcatcaaaaa ctcagttgct tatcgaaacg gctgggtacc
     2281 aggaattgag ggagaaggta acggtaatgg attcaaaatg ggcggttctt ctttaacggg
     2341 ccctcatgaa ttgatcaata gtttgtcttt cgaaaatctt gcgaaaggaa tcgattccaa
     2401 tagcggaaca gatattatcg tcaatagcag tacaagtttt aataacggtt catataatgt
     2461 ggctctttat acctcttcag caggaaacac agactaccat gcttctggta tcttatcttt
     2521 ccgaacagag aatcttgaga tgagagagca gatccgccca ttgaatcaaa acgaagatca
     2581 aatctaccac tcgttgaact actattggaa tgaacgagag aaacaatcag aaaatactct
     2641 tggacatacc atatcaaaag attggatcga gtctctaaat acatgttcga aagaaggtca
     2701 gcagcctgtt acgcgctatg aaaatggaag tatccatgtt catggactga tgcagattaa
     2761 accggggaat cgtcaaacag aaaaggaaag agtggatggt cttccattat ctgtaggtgc
     2821 tatttttgac ggagaaacct caccgtcttc ggaatatcca gagtatgcaa tcgtacaaga
     2881 accaaatcag acaaaaccaa atgaacaaaa taatccgatt attatacaag aagcaaatgg
     2941 tgtggcggag aatgcagaag gaaatatcat tgaactgaag ccttttacag tttttgtgtc
     3001 aactttcaca ccaaatcaag gaacgcctgt ctttgaagat atgcgttatt tcccagaaaa
     3061 gatccaaatc gttgaaacac agtcaagtgc tcaattttct aatcataccg ctattttttc
     3121 agcaccttca aaaggagaat atacgctaga agtcacttat cgttcggaaa tatttgatgg
     3181 ggaaaaatgg tctgctcata cagaatctgt tacccagcaa aaagcgatca ctgtgcgaga
     3241 aactgccgat gtgcccccgg gaaatgacaa accagaaaga gaaaatccag gaaatgaatc
     3301 accaaatgga aaaccaaatg gaaaaccttc tcatccggat aggacagcaa aagaaagtga
     3361 aaaaaatggc actcaaggac tcccaaaaac gggagaattt aataacccgt tgctagcatt
     3421 agctggagga attttgttaa tcggtgtagt tgtttatgtc atgaaacagc gaaaaaaata
     3481 agtggaattt ctccttttat gatgtaaaac aattgactga ggaaagtaaa aatagaagga
     3541 aatgagacca atagatgtct cacttccttc tatcttttta tagatgaggt tctccttgct
     3601 gcttttgatt ggctgttctt attacttgat gcaggacagc tttttcacaa tctctccact
     3661 aaacggtgtt caatcagctg accctcggta ttagctcaac aaacggcaaa atatgaggaa
     3721 ctatttttcc gtttttttct ctaatactca ggtcaggacg ctgatttcac aacctctcga
     3781 taaacggtgt tcaaaagcca gtttctcggt agtaagtcaa atttatgcaa aaatgtggaa
     3841 agccattttc acaaaatttt tcttactact tgaaactaag cagctttttc acaacctcta
     3901 atacatggta ttccagaagt aacttcttcg aaaaataagc cgaaatgtca caaaaatttg
     3961 agaagcgatt ttcccaaatt ccttcttatt tcttgaagct gaccacttct ggcacaacct
     4021 cttaccagat tcgcactctt ttttctggcg ctagatacat agcgtcttgt tccgtgatac
     4081 caaatgtttc atagaattcg tcaaggtttt ttggttgcac gtttgctcgg agtttagcag
     4141 gggcatggac atcgatttgc agtaaaagtt gttggtattc tttttttgct tttgtacgcc
     4201 aaatcgttgc ccaattgaca aagaattcct cgatagaaag atctgattcc gtttttgctg
     4261 cttccaatgc acagcttaga ccgcctgcat cagcaatatt ttccgaaacg gttaattttc
     4321 cgtttacttt tccgtcagca aaaggcagac catcgaattc ttcgatcatt gcttgtgctt
     4381 tgttttgaaa ttgtgtaaga tcttcttctg tccaccagtt gttcaagttg ccatattcgt
     4441 caaataaagc gccgttatta tcaaatgcat gggaaatctc atgagcgata acagctccga
     4501 ttccaccata attagcagag cttgattgtt ctagactgta aaatggtgct tgaaggatcg
     4561 ctgccggaaa gacgatcaca ttccgaaatg gatgataata agcattgacc gtgtcagcac
     4621 tcatttccca ttctgaacga tccactggtt tgttccattt gctgaaacga tcttttaatg
     4681 tgagtttgct gaaaacaaga gtatttgaaa gcaaagtacc accctgatct gcaggtactg
     4741 ttttgaactg gtcgaataat gctgggatct tatcggggta gcctacttgg atacctaagt
     4801 tatttaattt tatgacggct ttttcttttg ttgcatcccc aagccagtca ttttcttcta
     4861 gacgtttttt ataaacggcg atcatttttt tcaccatttg ttctacgtct tttttagcag
     4921 cttcgccaaa atattttttt ccgtagtaat cgccaactac ttgatcgaat tgtccagagg
     4981 caaggtaata cgctgctttt ttctgaggca ttgcttcgtc tgttccagac aatacgcgag
     5041 aatagattcc gctgacttgg cggaattctt ctgatagata accactaaga gaacgtactg
     5101 ttttaatgat catccagctt ttgaataacg gaaaatgagc ttctgtcagg atgtcattca
     5161 aatgatcgaa gtacactgga tcagttacga tcactttatc tggagttgtc ccaatcaaac
     5221 ccgtgacaag ttttttcaag tccagtttat ctgtgtattt aataaatgta tcaaaagatt
     5281 gcggattata cattttgctg taatctgcgt tttcttctgc actttttaca tgaggagcaa
     5341 tcaatttatc aaattgcact gcttgttccg tgattgcttc tgcctgactt ttttctttgc
     5401 cggcaagctc taataactga acagccatat caaagaaaac gcctattagt tgtgcaccgt
     5461 tgggatggtc tgatgcataa tacgtcttat ctggaaggaa taaagaaggc ggataagcaa
     5521 agaaggcgtt cgtttgcgca ttcttcatat ctgcatctac atccagatta aatggcaatg
     5581 gtaatgtatc caacagccat tctggaaatt gcttattcaa ttcgtcgaaa gactgcaatg
     5641 attcaacttt ttcgattata ggttgaagcg gttgagcatg caactcattt cttcggtcat
     5701 aatcgtttgc cagttgataa tatgcaagga aatgtttcat tttttctgaa gacacttttt
     5761 ctggatgggc aagcagatcg tctgtttctc ccatcaaaag ttggtcgatc ccgtcaacta
     5821 aatcttggaa accgcctgta gctggtttat ctgcagggat ttttgctgtt ttttgccatt
     5881 cttcattgac tgcttcatag aaatcttgtt tcaatcgttc tttgttcatt catcgatcgc
     5941 tccttttttg tttcgtgtgc ccattatctt acaaaatgaa ggaattaaca aatagaacat
     6001 aaaaaattga aaattcgaac aatcaaactg atttgttttt ttcaatcctt cacttataat
     6061 gaaaaaaagg aggcgatccc aactgaatca agtagtggat aaacctttat atttcaaagt
     6121 agataaaaaa attaaaaaat tagccagcac tcagcagctg cagtcaagaa aaagtaaatt
     6181 gcttttttta gctctggttt ttgaagatca aagttatgtg attatcgatc aatcaggaca
     6241 tccggtagaa tattctccag caaaatatac ttatcaagag ggtctttctt gtaaaaaatg
     6301 gaagctgatc aatgaagagc caatcgagtt atctagatgg atcaacagaa aagaagatat
     6361 tcccgttttg atcgaagaaa aaaggtcaga aaaggaactc gcaaattgct gggtgggatt
     6421 gccagaagaa cgttttttga ggtataagaa atgggctacc ccctcaggct atctttgtgg
     6481 tacttacgcg gcagcagttc tccttgccta ttatcaagac tatcgtaagg ggtggatgct
     6541 tccaaatgag attcgcaaga aaaatacggc tgattcgttg gttttgacga aggcattgcg
     6601 tagccagatc caaccactag gacttcctac gattcctttt caggttagta cagggatttc
     6661 tagtttttta aagaagaatg gcaatcatga gcgtgcacga gcaactcttc taggttcatg
     6721 gcagcgagct acaaaacgta tacgtgaagg caaacctgtg atgattggta ttttgaaagt
     6781 acttggcagt acatatggca atcattgggt gacagcgtac gcttattttg aaactgaaac
     6841 aggagaacgt tattacaaag tacacgacaa ctggggcgat tatcataaag tcattcctgc
     6901 cagttggagt aacggaacag tttcacttcc ttaagaattt gctgaaatta acagaaaatc
     6961 ttggaaaaat atgttaaact atcgaagaat agagaagttt tggggaggac tacatgagta
     7021 ataagtatca ggatgacacg ttgaagattt ttagtttgaa tggcaaccgt ccattagctg
     7081 aaaaaatcgc gaaagtgttc ggaacagaat taggtaaaag cgtcgtgaag caatttagtg
     7141 atggcgagat ttccatcaat atcgaagaaa gtattcgagg ggatcatgtg tatatcgttc
     7201 aatcaacgaa tgcacctgtc aatgattact atatggaact actgatcatg atcgatgcaa
     7261 tgaagcgagc aagtgcgaaa acgatcaatg tcgtattgcc ttactatggg tatgctaggc
     7321 aagaccgcac ggcgaaacct catgaaccga tcactgcgaa attgatcgct aacttgatcg
     7381 aagaagcagg ggcaactcga gtactgactt tagatctgca cacggttcaa gtacaaggat
     7441 tctttgatat tcctgtagat aatttattta cgatgccgtt gtttgctcat tattaccgtc
     7501 aattaggtct gacgggagat gacatcgtag ttgtttcgcc aaaaaacagc ggtgttcagc
     7561 gtgcacgtag tctttcagaa tatttgaaca gcacattagc catcgtagat catgcagatg
     7621 atgcttcaac tgagggtggt tatgtgatcg gtgatgttca aggaaagaca tgtatcatgg
     7681 tagatgatat cttgaacaca ggagcaacgc ttgcacgagc agcaaatgta ttaaaagaaa
     7741 acggcgcaaa agaagtatat gcctgtgcgt cacatggatt attgtccgat catgcgaaaa
     7801 gtattttaga caatgcacca atcaaagata tttgtatcac tgattctgtt ttcacagaag
     7861 aaaatcgtca tccagaaaac ttaacaatcg ttacatgttc agaattaatg ggtgaagcgg
     7921 tcaaacgcat ccatgaaaat acaccgatga gtccgttatt ccgtctggaa gaaaaagaat
     7981 tcgaataaac aaaaaaaaga gggaaagaag ttgtgacaga agtgagcagc ttcaaggagc
     8041 tgcgacaaga cttttgtcac agctcctttt ttctatgccc atgaaacgtc ctctagacaa
     8101 aaatgagctc ttctttggtt tgttcgctat tttatgctag aatgtgccta gcaataattt
     8161 ggaagggatg aatgaagtga aaactattta tctggatcat gcggcaacta cccccatgca
     8221 tccacaagta attgaagcga tgacggcaat catgcaagac acgtttggaa atccttcaag
     8281 catacatgga tttggtcgcc acgcgcatga aaaattggaa acagcaagac aaatcattgc
     8341 agacagtctg caagcaaaac ctcacgagat catctttaac agtgggggaa cagaagggga
     8401 taatacagct atcttagaaa cagcgttctc gcgtcaaaaa gaaggcagac atctaatcac
     8461 tacagctatc gaacatcctg ctgttcttaa taccatgaaa tatttagaga caaaggggtt
     8521 tgacgttaca tatctgcctg tagatgaaaa agggaatctg tcgatcgaag aggtcaaaaa
     8581 agcgttacga gaagatacga tcttggtctc tgtgatgtac gggaacaatg aaatcggaaa
     8641 tttgatgcca atcaaagaaa tcggtgaact tttagcggat catccagctt atttccatac
     8701 agatgcggta caagcgtacg gaaaccaaac gatccatcca gaagaattag gaatcgattt
     8761 attaagtatc tctgctcata aaatcaatgg gccaaaaggc gtcggtttct tatataaacg
     8821 agacggagta aatatccctg cattgctttt aggcggggag caggaagaaa agcggcgtgc
     8881 aggtacagaa aatttagcag ggatctgggg tatggcaaaa gcagttgaac tgctgtcacc
     8941 tgaagaacgt gaagcacgta acaacaaata taaaggattc cagcaattga ttcttgatac
     9001 attagaagca aacgaaatcc cttatcaagt aaatggtgat ttaacgaata aactccctca
     9061 tgtgttgaat cttcgcttaa ttggcgaagc aaatgatttg ctactgatga agcttgatct
     9121 taatggggtc gctatttcta ctggttcagc ttgtacagca ggaaatatcg agccttcaca
     9181 cgtattagaa gcaatgtatg gagaagatac accgatacta aaagaatcca tccgtatcag
     9241 ctttggtttg ggaaataccg aagacgacat ccagtatttc cttgatcaat tagttcgtgc
     9301 tgtcaaaaaa taactagtga agtcattctt atatgaacag atgtctgaga gaacgccttc
     9361 attttttagg gaacttaact gaaaaaccta gtaaaaatca ggataaactt ttataataaa
     9421 gagaaactaa ttttatcgag gtgaaaatca tggcatttga aacaacagca acggtactgg
     9481 gcgcaccaat tagctatcgt ttgcatcctg atgcaaaaag atatacattg cgcgataatg
     9541 gatttacaga aacaaacgga ggcaattatc aattgatccg accattagat gccacgccgc
     9601 aaagcaaaga aggattcaaa ttaaaaatca ctgtatcaaa agatattcaa acattgaaaa
     9661 tgtcgatcac tacagcaaat ggattgaaag cagtcaatat tttcaaagat ccaaaacaga
     9721 aaatgagcca agataaattt tattttctga tggatgggat gattagccgt gggttattcg
     9781 aaaaagtcga atgatctgtg gatataaaaa gacaactagt tttgaaacgg cctattgtta
     9841 gatgactaac tttgtaagga cgacgcaaat tagttgtctt ttttattttt aaatttaaca
     9901 catgttgtta tttttaatat tttgctgatt tattgtttct aagtaaagaa atttaaattg
     9961 aaacgattgt tatttaggga aaaaggtatt ttttgaaaaa agaatcccgt ataatgattg
    10021 ttgtgcaaac gctatacaaa atgaaaaaga aaagagggat gtttatggaa aatgaacagt
    10081 cggtcgtttt aacgaattgg aagagaaatt atttgttctt tttatcggga cagtttttgt
    10141 caggaatcac tagtatggtc gtccaatatg cgatcatctg gtatttgaca agagaaaccg
    10201 gttcggcgac aattttgagt tttgctactt tactaggaat gattcccatg gttttactaa
    10261 gcccatttgt aggaccgcta gtggatcgct gggataaaaa agctcttttg attgtcacgg
    10321 atattattgt agcgattttc gcgcttattt tagcagtagt cggaacgatt tcagaatcct
    10381 tcccgatttg gctagttttt gtgtctttgt ttatgcgttc ggtcgcacaa acattccaaa
    10441 tgccaacgat ccagtcgatt atgccgacaa tcgttccatc ttctcacata acaaggacga
    10501 atggacagtt aggaatggtc caatcagcga atttcatcat tgcgccagct ctaggtgcgg
    10561 ctttgttttc agtcgttcct gtcaattacc ttattttatt agatgtattg ggggcagttt
    10621 ttggtgtcgg cttattgatt tttgtgaaga tcccaaaagt ttctcctgaa atattggaag
    10681 ttccgcttac tatttttaaa gatgcaaaat ttgggctgca gcaattgatg gataataaag
    10741 ggttatggta cataacgatc aatggtgcat ttgtgatgct gttgttcatg ccagcaatca
    10801 gtctgtatcc attgatgacg ctagattact ttggtggttc cgttggacag gcgggagcag
    10861 tggaagtagt ctacgcagtc ggaatgctct taggcggtgc actgatcagt tttataggaa
    10921 catggaaaga ccgaatgaaa cctattatta tagcctacat cattatgggc ctgacgatcg
    10981 gtgcaagcgg attggttcca aacgatagtc aagggttctt gtactttctt atcttgaatg
    11041 caggagcagg ttgtgcaacg ccatatttca atacactgct gatggcaatg atccagcaga
    11101 gttatgaatc gaatgtttta ggtcgtgtct tagggaactt caactctttg atgaacctgg
    11161 caggtccaat tggattatta tttgcaggac cattagctga tcgtttaggg gtagaaaaaa
    11221 tgttcttgtt ttccggaatc gggattttat tatgcggaat cgttttattt ttaacgtcag
    11281 ctgcccgaaa ttatgataaa gaattacaga aaaaactggt aaaagaacac catgagcaaa
    11341 aagacgaata gtatggaatg gagttgtgac agaagtcttg ttacaactcc atttctgatc
    11401 ctaatgttcc tgtggcaata agcctactca aatgaaaaat atgagtaatt atctttattt
    11461 gtttaatact tgatataggg cagttttttc acgacctatc gtacttcctc tttgcttcat
    11521 agatttcttt gatttgttgc tcagataacg gagtgccaaa tggtagtttt tcctttaaga
    11581 gttctctttc cggtgcgtct acgcctacat tgacatcgtt ctcgataggt acgctgtaat
    11641 gatggatatg tccatgaaga ttgcggatag tgtcgttagg cccgagcaac atcggataat
    11701 gagtcaagta atattgttga tggtcgaatt taacgatggc acctacatca taaaaataaa
    11761 atttgggact tccgtcagta agcctaggat catttttttc taaataacgg aaaaaagcgc
    11821 gactgtcatg gttccctttg atcaagtgga ttgttccttg cagttgagtg accagttcca
    11881 aaatttctgc atttgctttt tcatattgcg gatgcatagc aaggtcgcct agatgataaa
    11941 catggtcggt ttcttttatt actgcattcc agctagtgat caactgttgg ttcatttcat
    12001 ccacatttgc aaagagccgc ggagcaaagt cgttttttcc taataagcgt tcatggaaaa
    12061 agtgcatatc actgataaag taattcatgt atcttttgcc tccctttttt cttagagtaa
    12121 acaaaatcac ttatctgagc aactcataac atctaaaaag aaaaggttgt aaatgagacg
    12181 tgcagcttta agcgataaga agcaattttg aaaaatagtt tttcacaatt tataaaatta
    12241 ttccttattg ccgaaaggct ggcccttttt atgagtatgc caatttttta tatcgaatgc
    12301 tcaaaagatg agtgattttt tggttgacta tcatgtttgt gtactgaata ggtccagtag
    12361 aacagtattt ttacgacttg ataattaagg gatgaaattt tcagaaatat gacgtataat
    12421 gtaaaatact gaaaagttgc gaccttcaac tcgtagatgt ttcagaatct aattgaaatg
    12481 atggtgatat catgacagac aacagcaaaa cacgcgttgt cgtagggatg agcggcggtg
    12541 tcgactcatc tgtaacagca cttttgttaa aggaacaagg atacgacgtg atcggaatat
    12601 tcatgaaaaa ttgggatgac acggacgaaa acggtgtatg cacggccacg gaagattata
    12661 aggatgtagc gaaagtagct gctcaaatcg ggattcctta ttactctgtg aattttgaaa
    12721 aagaatattg ggatcgtgta tttgaatatt tcctagccga ataccgcgca ggacgtacac
    12781 ctaacccaga cgtgatgtgc aacaaagaaa tcaaattcaa agcattcttg gattatgcaa
    12841 tggatcttgg cgccgattat gtagctactg gacattatgc gcaagtcact cgcgatgaaa
    12901 acggcgtggt ccacatgctt cgtggagtag acaatgataa agatcaaact tacttcctta
    12961 gccaattatc acaagaacag ctttcaaaaa cgatgttccc tctaggcggc atggaaaaat
    13021 cagaagtacg ggcaattgct gaacgcgcag gccttgcgac tgctaagaaa aaagattcaa
    13081 cgggaatctg ctttattggt gaaaagaact tcaaacaatt tttgagcaac tatctgccag
    13141 caaaaaaagg aaatatggtc actcttgatg gtgaagtcaa aggacaacat gctggcttga
    13201 tgtattatac gattggtcaa cgtcaaggac tagggatcgg tggcggagga gattcgcaag
    13261 aaccctggtt tgttgtagga aaagatctag cgacaaacac attatatgtg gggcaagggt
    13321 tccatcatcc tgcattgtat gccacaagct tggatgcaag tgaaatccac tttacgacga
    13381 atgaaccaat gccaaaagaa ttcaagtgta cagcgaaatt ccgttatcgt cagcaagacg
    13441 ttcctgtgac agttcgctta ttggatgata atcgtgcaga agtcgtattc gacgaacctg
    13501 tacgtgcaat cactcctgga caagcagttg tattttatga tggaatggaa tgtctgggtg
    13561 gtggtttgat cgaccacgca tatcaagaaa caaaagtttt acaatacgtt taagcgatat
    13621 taaatgaatc aaaacagcta tggctaacaa aagaaacacc ttttcttttg gatagcatag
    13681 ctgttttttt gcaataaaat taatttttat tcattttata cttgaaatac ttgtgtaaaa
    13741 agttcataat atccaataat atgaataaag ttgctttttg ttaagcaaga gcagttgcaa
    13801 aatagaaggg aatcttttgc aagtgatttg ttcaatttta taagtgaggg tgatggaaat
    13861 ggaaaagaaa tcgacgggta ttagcaaatg gggactggtt gctctggttg tcagttcgtc
    13921 aatcggaagc ggtgtgtttg ggatcacaag tgatctggct agttctgcag ctccagggcc
    13981 agcgatcctt tcatggataa tcgttgggat tggtatttta gctttggtat tatcgctgaa
    14041 tcatttagga gaaaagcgtc cagatctaga tggtggtatt ttcggttacg cggaagcttc
    14101 atttggtaaa ttaggtggct tcatcagtgg ttggggttac tggttgtctg cgtggctggg
    14161 aaatgttgct tttgcaacga tgctgatgag cgccatggga gaatttttcc cgatattcaa
    14221 aggtggacaa aatgttcctt ctattttatt tgcaagtatt tttatttggg gtctgacttt
    14281 attagtaaac aatggagttg aaggagcgag cgtcatcaat acaatcgtga cgatctgtaa
    14341 attagtgcca ttattcctat ttttaatatt tgtatttatc gcatttaaag tgaatatatt
    14401 cactgctgat ttttggggta acgtatctga taatctagtg aatggaacag ggcagcaagg
    14461 aacgatctgg ttgcagatta aaggttgttt gatggttatg atgtgggtct ttgtagggat
    14521 cgaaggcgca agtgttttag ccaaccgcgc gaagaaacgt tcagaagccc agcaagcatc
    14581 gatcattggt ctgctttgtt tattgtttat ctatatcctt gcttcgatct tgccatacgg
    14641 cgtcttatct caagaagagt tagcaactat ttcacaacct gctatggcaa atattttaaa
    14701 aggaatcgtg ggaaattggg gagcagcgct gatcaatatc ggcttgatta tttcgatcat
    14761 tggttcttgg ttgtcttgga cgatgctccc agcagaaaca acgatgttga tggctcgaga
    14821 cggtctgttg ccgaaaggtt ggggaaaaac gaataaaaaa aatgccccaa cttattcgtt
    14881 agttgttact gcaatcttga ctaatctatt cttattgact ttcttagtta ctgattatgc
    14941 ctatcaattt gcctattctt tatgtacagc ggcaattttg atttgttatc tacttgtcgg
    15001 gatctatcaa gtccagtttt ctttaaaaga aggaatcaaa aaacaagtag tgatcggtgg
    15061 gattgctgta gtttttgaat tgatgggcat cattttagca ggattttctt atgtcctttt
    15121 atgtagtatt gcctatcttc ctggtttttg cttctactgg atcgcttgtc gtgaaaccaa
    15181 acatcaaatt acgggaaaag aaaaagtaat gattgggctg atttgtgtgg ccgcactgct
    15241 ttcaatcggg ttgcttgctg taggctggat caagatataa gggggaaaga aacatgaaaa
    15301 tcgcaaattt cggtgtagaa gaatggctaa ataaatggga aaagaaagcg gtatatgata
    15361 ttgcacaaag ctcgatcgaa gcattaacat tagacgaact cgttggctta gatgggacta
    15421 gcgtaagtaa atttttcgaa aaacgcaaaa acgaaccatt ggactatggc tggatcgaag
    15481 gatcagatgt tttcaaacaa gaagtcgctt ctctttatca aaccgtcgat ccggaaaaca
    15541 tcttacaaac gaatggagcg actggtgcta atcttttagc tctatacgcg ttagtggaag
    15601 caggagacca tgttgtttcg atgcttccta cttatcagca attatatgac atccccaaat
    15661 ctttaggtgc cgctgttgac tttgttcatt tgaaggaaga agaagattgg cagtttgatt
    15721 tagaacagct agaagcctta gtcaaacctg aaacaaaaat catctgtttg aatagtgcaa
    15781 acaatcctac aggcacattg ctagatcgga aaactttaga aaaaatcgca gaaattgctc
    15841 gtacagtaga tgcatatgta ttgatagatg aagtatatgc gcctttaacg gataaaggtg
    15901 aattcttgtc gatcgtagat gtctatgaaa aaggtatagc aaccaattcc ctatccaaaa
    15961 cctactccat tccaggtatt cgtattggct ggacagctac tggacctgag ttggcagaag
    16021 tttttcgaaa gtatcgagac tacacgatga tttgcggcgg agtcctgtca gatgatcttg
    16081 ctgtacatgc attgaagaat aaagaaaaaa tattggcgcg aaacaaaaag atcattacag
    16141 agaaccttgt gattttaaaa caatgggtag ccaatgaacc gaaagttgaa cttgtcgctc
    16201 ccaattacgt ctctacttct ttcattaaat tagccatcga agaagatgac cagacatttt
    16261 gtatcaattt attagaagaa acaggagtac tattagtacc aggaagtgcc tttgatttac
    16321 cgaagcatgc ccgactaggt tactgttgta aaaaagaaac cttggaaaag ggattattcc
    16381 tcttatctga gttcttgaaa aaacaggcct aaacaaaaac actgcaagga cagtctgtga
    16441 caactgttca agcagtgttt ttcatttacg cttattgtct atgagaagga gagtgccctg
    16501 aagctatgga gactcctgaa gctttttctt ctgcagtttc ttgctgatgg atctcttgtg
    16561 tattgtgtcg tacaagcaag aagttgacca aaacaagaag agaagtcgaa agaaagacgc
    16621 cacgataacc aaatgcactg gaaacactgg agccgatcat aggccctact acgttaccag
    16681 ttgcttggaa agattggtta taactgaaga tccgtccagc agcttgatga ggcgaatatt
    16741 ttgtgatcaa tgcttggaca gcagggagca gacaagcatc agagatacca atcaaaaaac
    16801 gtaaggcagc taattgccaa acattcgtga cgaatgccat tgggatataa accagaatcg
    16861 caaaacccaa accaatcgca agtattcttt cactcccaat cttgtcacct aagcgaccaa
    16921 atcttggagc ggctatcaaa gtggcaatcc caggtatcga agcaatgatt ccgctgatca
    16981 acgtaacatt cccatgtcca tgcagcaatt ggcgaatgta cagactgatg attggactaa
    17041 tggaattgtt ggatgcttgg atgatcatcg ttgtgacgaa cataccgatg acgacgtgtg
    17101 gatatttcaa gtctttgaaa atctgtttgg cagaaaccat gttttctttt ttcactggga
    17161 caaatttttc atggacgaaa aacagactca acaagaagac gaaaaataag atgatccctg
    17221 taatgaaaaa ggtagggcga tagccaaacg ctgaagcaga gatcccgcca agaagcggtc
    17281 caagtaatgt tcctgtaaca gaaccagttg ctaaggttcc aagaacttga ccactttttt
    17341 cttttggcgt cccagttgcg acaagggcag tggcattact gatataacca gaaaaaatcc
    17401 cttgaagcag acgcaatgcg actaattgat acacactagt cacgcaaccc atcaaagaaa
    17461 tgacgattgc cattcctaat gaagcacgca acagcatcaa ttttcttcct ttttggtcgg
    17521 ctaattttcc ccaccaagga gagataagcg tagtcaccaa gaaagtagat gaaaaagtca
    17581 atccgctcca aaagttcaat tgtgacgtac tataattacc taacgtatct atgtaaagtg
    17641 acataaaagg catgactaaa ctaaatccga taccagccat aaatgttcca aaccataaga
    17701 caaacaagtt ttgttcccaa gtttctcttt tcctaaataa tttttccttt attcttgcag
    17761 ccaattagac tccttctttc tgaaaatttt catctacagc tctttattat aaactacaat
    17821 atgtagttta tcaatgaaag tctctttttt ctctttcttt ttcgtggtaa actgaaagag
    17881 tgaaggattt attttatgag aggaaacatg gaaatgaaaa aaagaaatct gtctcaagag
    17941 aaaatcatcg actgctttcg agaactagca gaagagatgg atgttcagca aataaccttt
    18001 cagcatttag ccaaacgctt ggatatcaag gctccctcgc tgtataacca tttcaaaaat
    18061 attcgtgatg tcaaaacagc gctaacagcg aaacttctgc aggaacttaa tgaccagctg
    18121 cgacgggctc ttgtgggaaa aagtggtcag gaagcactgc agatctatgc agaaacttat
    18181 cgatcctttg ctttttcaaa tcaagcagtg tatgagttac tgatcagtgt accgcatacc
    18241 aatgaagctg ttttattaga tggaatccat gaaacgaatc agatcatttt gcagatattt
    18301 gaggcttttc aattaacgaa agaagaaaaa gttcatcgaa gcagggaact tcgtagtatg
    18361 atccacggtt atctttcctt acgtttttta ggatacttta caaaagaacc aacaatcgat
    18421 ccagaagaca gctatttttg gatgatcaat aactttatcg ctactttgcc ttaaacaatc
    18481 agattcattt gtaacaattt taagatgttc attaaatgac ccttttataa gagaaaattc
    18541 ttgcaacttt cttgaaaagt gactaagatg agtctagaaa agctgaaaga aggagcacga
    18601 gtatgtatca agtaattacc atgttcgggg acaatgagcc ttggtggttt tttgaagact
    18661 gggaagaaga tattgaagaa gaagaaacat tcgagtcttt cgaagaagca cgacaagcgt
    18721 acgaaaaaca atggaacgaa atccatcaaa attatgaata tatcaatgca aaaagtaact
    18781 ttctcagcgc cttttggaat gacggagatg agcgctggtg tgaagagtgt gacgatgatt
    18841 tacagcaata caagggactt gcgttgttga aaaatcatca gcccatcaca gtagaaagca
    18901 gaaaggaatt ttatgaaaca actaattcta gcggaaaaac caagcgttgc gaaagaccta
    18961 agcaaggtgc ttggtgctaa tcaaaagcac aaaaattatt atgaaggacc caaggtgatc
    19021 gttacttggg cccttggtca tttattagga ttgaaaatgc cggaagatct gaataaggaa
    19081 tggcagagct ggcaaatgga cacgttgcca atgatcccta aaaaactagg aatcaaacca
    19141 cttcctaaaa caggacatca attaaaagca atcaagcagc tggctcagcg aaaagatgtc
    19201 tcagaagcag tcattgcgac agatgccgga agagaagggg aacttgttgc tcgctggatc
    19261 ttagagtatg tgcatttcaa taaaccagtg aagcggttat ggatctcttc tcaaacagat
    19321 aaagcaatca aaactggatt caagcaacta agacctgcga aagaatatga tgcattgtat
    19381 gaatctgctc ttgcccgggc aaaagcggac tggctggtag gattgaatgt gaccagagcc
    19441 ttaactgtga agtacgcgga taatttatca gcaggccgtg tgcagacacc gacactagca
    19501 ttagtgagag accaagaaaa gaaaatcgag cagttccgtc cacaaacgta tttcacgatc
    19561 acgttgcaag ctggaaaaga acaagccaaa atgcttcaaa aaaatcaatt tgctttaaaa
    19621 acacaagaag aagcacaaaa acttgttcaa ttgatcagta caagcaaagg aactgtcaca
    19681 gaagtccaag aaaaattaaa aacggaatct gcgccattgc cttatgattt aacagagatc
    19741 caaagagaag ccaatcaacg ctatggctat tctgcaaaga aaacattaag tcttgtacaa
    19801 agtttgtatg agacccataa gatcgttact tatccgcgta cagacagcaa atatctgaca
    19861 aatgacatga aaagtacgat gaaagaacga ttgcaagcag tcagcgactt tgctcctgag
    19921 gtcaagcagt atttgaaaaa cggagccatc gtccaacaga caaaagtatt ccaagacagt
    19981 aaagtgaccg atcaccatgc acttttgcca actgaaaaca gagcacgtta tgaaaaatta
    20041 agtaatgaag agcgaaaaat ctatcaaatg attgtctctc gttttttagg actgtttgct
    20101 caaccccata aagtcagcca aacaaaagtt acggttgaat ttggtaaaga acgattcatt
    20161 tttcggcaaa atcgtgtcgt ccaagcagga tggaaaggcg aaactgaaac tgagacggaa
    20221 acagtcaaat gggaaaaagg aatgagaatc aatcccgatt tcgccatcaa aaaagaacta
    20281 tctgcaccgc ctaaaccatt gtcagaagca agcttgcttg gtcaaatgga aaaatacagt
    20341 ctgggaaccc cagctacgcg tgcagagatt atcgagaaac taatcaaatc tgaactgatg
    20401 gaacgtaccc ctcaaggact gcaagtatct cctaaaggta agcaattatt agtattggta
    20461 aatccctctt tagtaacacc agatttgaca gaaagctggg aaaaagacct tgaagctatt
    20521 gctgcaggaa agaaacaagc aggaaccttc ttgaatggaa tcgaaaaaga aacaaaacgc
    20581 ttggtgaatg agatcaaatc aagcaaacaa gaatatcaag atttctcgat tacccaaaag
    20641 aaatgcccag aatgtggggc caatctgcgc gagaaaaaca cacgtgacgg caaaatctat
    20701 gtctgcacca attcagattg caactaccgc agaagaaaag atccaaaagt ctctaatcat
    20761 cgatgcccgc aatgtcaccg gaaaatggaa atcatcgaag gtaaaaacgg tgcttatttc
    20821 cgttgtaaat acgatggaac gacagaaaaa ttgcttgata aaaaagagca gaagaagaaa
    20881 atgaccaaac atgaagaacg acgcttgctg aaaaaatatg cacaagcaga agaaccagaa
    20941 gaaagcccat tagcagcagc actaaaagca gcaatgaaga agtaataaga tatgaaagag
    21001 gttgtgatag aagtggtcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttctca
    21061 aatttttgtg aaatttcggc ttatttccga agaagctact tctggaacac catttattcg
    21121 gaaaaaagga ctgtgacaag acttttgtca cagtcctttt tcatttaaaa aaatgtttac
    21181 ttcatttgac gtatattatt ggtgaggctt caattcattt ggcttcactt tttctgtttc
    21241 tcttatgata taaacaggac gctgtttcgt ttctaagaag attttcccaa tatatttccc
    21301 gatgattcca agacataaca attggattcc accaatgaac aggaagatcg agaccatgga
    21361 aggccatcct gatgtaggat caccaaacaa caatgcccgc aaaataatga acaaaattgc
    21421 aataccagaa ccgacacaag acaaagcgcc gataaatgaa gcaatcgtta acggtgcatc
    21481 cgagaagttc acgataccat caatcgagta gttgaataat ttccagaagg accaagatgt
    21541 ttcgccagcc acacgttcgc ggttttcata agaaatatat tctgttttaa atccgaccca
    21601 gctaaataat ccttttgaaa atcggttgta ttctgttaat tctaggacgg catccaccat
    21661 ttgacgagtc atcaagcgga aatcacgagc gccatcgacc atttctgtat cgctgatttt
    21721 attgatcaac tggtagaatt ttttcgcaaa gaaactgcgt atcgcaggct cgccttcacg
    21781 agtcgaacgc cgggtgccga cacaatcgat atcagaagta gtgatccggt tgatcatctc
    21841 aggaagcagt tcgggcggat cttgcagatc aacatccatc acggtcacta aatctcctgt
    21901 agaagcttgc aatccagcgt acagagccgc ttctttccca aaatttctgg aaaatgagac
    21961 aaagcggaca taaggataag ctgaggctag cttcttgatt gctgctaacg taccgtcttt
    22021 tgatccgtcg tcaataaaca aatattcgaa ctcatgggca cctttgaatt tttcaacctc
    22081 atcaaaaaaa agaggaacag ttttttcttc attaaaacat gggacaacaa ttgatacttt
    22141 catcgtttct atcctttcaa tcgtatgttt atatatggtt tacattcaaa atttaataaa
    22201 ccttaaataa cacctttaaa atttgatttt acgtaaaaga acaggtattc tttttgtaaa
    22261 ctaacgtatt tagtttacca tgtttcctcg gtttatcaaa cttttaagaa caatttttat
    22321 tagttggcaa gtagttttgg atagagaaaa aggaggaagt tccagtctat tgactaggag
    22381 caaacaaaat gaaaatacac acacctatcg gctttttatg gttagatgca gatgaatccg
    22441 gattgactgc tgtatctttt cagccaattt cagaaagcga atcagcgaat cagcgaattt
    22501 taacgcgcac acaagaacag tttgaagaat attttgcggg aaaaagaaaa gaatttgatg
    22561 ttcctttatc tataaagaga ggtacgcctt ttcagcaaaa tgtctggaaa gctttgtcga
    22621 ccattcctta tggagaaatc cgaacgtata aagaagtagc cgttgctgtt gatagtccga
    22681 aagctgtgcg agcgattggg caggcaaatc gaaccaatcc attgccaatc gttattcctt
    22741 gtcatcgagt gatcgggcaa aatggaaaac tgacaggata tatgggaaac gcaatagatg
    22801 gggttgaaat caaacgccaa ttgttgttgt tagaaggata tctgcaaaat taaaaacagt
    22861 tgtgattcgg acattgttcc atgcgagaat cacaactgtt ttttcatgtt cattctaaac
    22921 tttgatcagc caaataaata gtaaagccat cactgtttgg agaagcccga cagctaatcc
    22981 ataagataag aagcgtgtac cttctttcat gaattttgca aagtctaaac gcagaccaat
    23041 tgccgctaaa gcagtgatct caaaccagct gctgataaaa tgtgccgatt ggtcaataac
    23101 tacaggtaaa gaaaacaagc tgttgaccaa acaaaagatc acaaacccta atacatacca
    23161 aggaagcggt aattttcgtt ttttctgcgt atcagttggc gtttcaaaca gagctggttg
    23221 tttcttgtgc atgaattttt caaacagata gacaacaaca acgagcatca taatacgtag
    23281 aatcttaaat agcatggcga attcgacaac agaattgtca agtaagcttg ccccagcgac
    23341 tacttgacca acagattgga gtgtaccacc caaaagtgca cttttttcaa tgagatttcc
    23401 accaaacaac tgtaatccaa gaaaagggag agtcaacatc atcacagtac ccagtagatt
    23461 gactagtgtg atgatctgtc ctttttcttc ctctttcgca tggatggaag gggcgattga
    23521 agcaatagca gaagaaccac aaacagcgtt tcctccagcc atcatcaaag aagaagtatc
    23581 gctgaattgc agttttttac caatcaagta agcaccgatg atagtgactg acatttgcag
    23641 taacacaaaa acaattcctt ttacaccaat ttgagcaatc gtttgagctg tgacagttgc
    23701 acctagtaaa aaaacagaga attctaatag tttgctttca gcaaattttg tgccgattgc
    23761 tagaacaggc tgtttaaaaa aagtatttcc taataaaata cccaataaaa tagcaatagt
    23821 cgctccacct aactgtggca gataaatagc aagaagcctg ctgaaggcag cgatggcaaa
    23881 agaaagcagt aaaccgggta agatttcgcg gaccgctgaa tcctttacag attttattgg
    23941 atgtgacaat gatttcattt gatcggttcc tttcgtcaat aaatactaaa agcatactcg
    24001 aacatagtag cactaaacca gtattttgac aaaagaacaa gaggtcgtga caaaagtctt
    24061 gtacgacctc ttgtttcgaa taaacggtgt tccagaagta gcttctacgg aaataagccg
    24121 aaatttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga
    24181 ccacttcttt tacgacctct tggtaaacgg tgttcaaaag cttgcatctg cggtaataag
    24241 cccaaccagt acaaaaacat gaacagctgt ttttgtttgg ccgttcttat tacttgatgc
    24301 agaacagctt tttcacaacc tattgaatcg attattaagc gattttttta gaaacaaatt
    24361 ttccatgttt gctatgttct ttaattaaga aacgtacgta atgagcaaga acgtagaaga
    24421 caactacact tgcaaacaga cattccgcaa cttttaagaa atccaatacg ctactataga
    24481 tacctaaact gttgaagtaa tgaacagtag taaatatagc tgttgcagag ataacaagcg
    24541 ggaatgtaaa ggctgcatag cttggataaa aacgaagctt gaacaaacgt ggaagcatag
    24601 acagcacaac gaaatacaat acttgagata aagcgaacaa tgctacgaca agataaaaat
    24661 taggttcagc aaagttttta agataacctg ttaggcaaag agaaccaggt gctgcaacga
    24721 tcgtaatcag cggacgtgtc ggatgagcca tatttcttac gaaaaagaca cgtttgatga
    24781 tgatcggcaa taagaacgca tagcagatca atgaaatcca aaagaaaatt tttccaataa
    24841 gtgggaagaa gttcccggca gttacagtga tgaccccaaa tccaacgtaa atgataaacc
    24901 agcttggata aattgcttca attgaaagat cagctttgat aacatggtga taattgaaat
    24961 aaaagacaag gatcacttgg aagataacag cgcacagcca aatgaatttg acaattggtg
    25021 caatactttc atatgaaaca aaataagtac aaataaccat gagtgacatc gtaaaagttg
    25081 gtgaaacgga ggcgatgatt ggatcatgca acgtttgttt cgtgtgttcg aataagataa
    25141 acaatttaac aaaaatgaat atcataagaa tcatagagat accgccagtt agattgccta
    25201 aagcaacatg atgatagtct ttaaacagat ttccaaggga agctaaccca aggatcaacc
    25261 cgcagatcgg gatcggaata attttcaaaa agttcttcat atataatatt ctccttttta
    25321 tttaatagat tttagtacca agatgaacga atacattgta aaatgaatag gaatgtatga
    25381 ttacgagcaa gcagacagct gattgtcttc atctaagtta ctagttggat cagcaactgt
    25441 tttgttgtac atttctatta tagatggttt tttgtatatt taaatcaaat agtttttata
    25501 gtaattatca taaaaagtta tggaagtggt gccatgttta agttattaac gacttttcga
    25561 gcagtatatg aaacaagaaa tttttccaga gcagcagaag tactgttttt atctcaacca
    25621 gcagtctcga atcaaatcaa acagttggag cgggaattga atatcgatct ttttcaacgt
    25681 aacggaagaa aagagatgct tccaactaag caagccgact tattatatga acggacactg
    25741 gttttgttag aagaatggga agaaactcag caaaaattgc tcaatgaaca aaatgaagaa
    25801 gaagtatgtc ggatcgttgc ttcccacacc tttgctgtct atcttttgcc tcagttgatg
    25861 aaacatctga tcaaaaggtt tcctaaagtg cattttgaga tcgaaatcac gaattctcat
    25921 gaatcattag aacaagtagc gaaacatgaa tccgattttg gttttatcga aaagccatta
    25981 gagacttccg gcgtacaaag atactctttg atggaagacc agctagtcat cgctggggac
    26041 ccaaaaagca aattgtggct tgtaagagaa gcaaactctg gtgtttttca ttacacgcag
    26101 cgttattttg aagagatgaa tatccaagat aagaagatga tcgtgaaaag taacgatgta
    26161 attgttgcgt tgctgaagga aggaatcggt cgctcgattt tgtctaagcg ctctgttcca
    26221 gaaaatgtgc caatgactcc gttgaatttc catcgatatt tttatttcat ccaacgaacc
    26281 aatctggttt ctgatcagct gctagagatt gccgaagaga tccttcgata caatgaaagc
    26341 acagaaactt ctcacagcga ggagaatctc agctggtaga taattgcatg tatcaaaaaa
    26401 agttctatga tacactaaag ataagcagta ggcttttgta ctgcatttgc tcaagcaact
    26461 gcacaaacgg aaagttaatt tttcccaaat gtctatggaa agactttcca ttatctaact
    26521 ggaccgctat aataatcagc agtatggttt ttaagaaaag ggatgaaaca tggaaaaaaa
    26581 attaaaagct tcgaacgata atcgtattga ttatggcgtg attttaccag tctttctgtt
    26641 atgtttgatt ggtctgctct cattatatgt agcacttagc catgatccga acaatccagc
    26701 agtcttgaaa ggcgtaggga tgcaagttgt ctggtattta gttggagcgg cagcaatcgt
    26761 tgtcatcatg catttgaatt ccaaatggat ctggaaactg actccttacc tttatggttt
    26821 ggggttagtg gtaatgggac tcttgctcaa attctatgat cagaatttag ctgcttcaac
    26881 agggtctaaa aactggtttc gattcggtgc gtttactttc cagccagcag agttgatgaa
    26941 gattgcgtac atcttgatga tggcactgat cgtaactaaa cataatacgc aagtaaaaga
    27001 acggacgatg aaatcggact tttggctgat tgggaagctc ttgatcgtga caataccggt
    27061 tttagcattg atcatggccc aagatgactt tggaacgatg cttgtctttc ttgcgatctt
    27121 tggcgggatt ttcctgatgt ctggaatctc ttggcgcatc atcgcacctg tagtaatctt
    27181 ggcagtagtc gttggagcag gaacgatctt cttggttacg actgaaggcg gaagagactt
    27241 gctttataaa gttggattca aatcttatca gtttgcacgt attgattcat ggctggaccc
    27301 tttccatgat acgtcaggaa tgagttatca gccggcccaa ggacttttgg caattggtac
    27361 cggtggcttg tttggtaaag gattcaacgt aagcaatatc tatgtgcctg ttcgtgagtc
    27421 cgatatgatt tttaccgtta tcggtgaaaa cttcggcttt atcggtggtg cattcgtcat
    27481 tttcttgtat tttattttaa tctatcgtat gattcgggtg tgtttcgata cgaataatga
    27541 attttacgcc tatatcgcaa gcggtttgat catgatgctg ctgttccatg tctttgagaa
    27601 tattggagca aatatcggat tacttccttt aacagggatc ccattgcctt tcattagtca
    27661 gggaggatcc tcgattttag ggaacatgat cggtatcggg ttgatattat cgatgagata
    27721 ccaaaatgaa gcacctgtaa gatcttcaag gaggtaagaa aattgtacac cttttactgg
    27781 tacccgaaat gttcaacatg caaaaaagcc aaagcgtggt tagaccagca tggcgttagt
    27841 tatcaaaccg ttgatatgat caaagaagta ccgaaggcct caacgattga gacttggcta
    27901 gaaacgagtc aagtaccaat ccgtcgcctg ttcaacacaa gcgggatgaa atatcgggaa
    27961 cttggactga aagatcaagt aggcgagttt acggcttcaa aagcgagcca agtcttatcg
    28021 acagatggta tgctgatcaa gcgaccactg atcttacgag aggaccagct tgttgcaatt
    28081 ggattcaatg aaaaaattta tgaaggagaa ctgatataaa atggcaggca agcatttgaa
    28141 gaaaaaggac aacctttgga tcttatttaa tggaaaagaa tactgtgttg gtttgacaaa
    28201 tgaagcgcaa gaagaacttg gagacatcac gtttgccaat ctgccaaaag caggacaatc
    28261 gctaaaaaaa gatgaacctc tgatcgaagt tgaagcggaa aaagcggtca atgaatttgc
    28321 cagcccgcta acgggtacag tttcttctgt taatgataaa atcggtgaag atatcaatat
    28381 cttgaatgat tcagatgaaa tgaatgcgtg gatcttaagt ttcaaagatg ttgatccaaa
    28441 agaatttgac gaattataaa atcagaaaat cacttgaaaa aagttgacac tgattcttcc
    28501 agttgctata atcataagca atcctatatg aaacgagaaa gctttgaaaa gaagagtaca
    28561 tgtcacggac gtttgaagag agccctgttt gctgaaaatg ggtaacgaaa atgagatgga
    28621 agatggtctt agagcagagt aagcgagcgt aagcaaactt actacgggag tgcccgttac
    28681 agcgcgacag tatacggaaa gaagctaagt ccaagtactg gaagaggctg ttattgcgaa
    28741 gtaacggtaa atcaaggtgg taacacggaa gttcaagctt tcgtcctttt gcttaaggca
    28801 agagggcgaa ggcttttttg tttgaaaaat cataagagaa agatgtgaat ggcatgtcat
    28861 taatccaact aacgaatgtc aaaaagacat tcaatgggaa aaacggaaac atccaagccg
    28921 tcaatgacgt ttcactaaac gttgaaaaag gggatattta tggaatcgtc ggctattctg
    28981 gcgcaggaaa aagtacatta gtccgtctcc tgaatggact tgagcttccc acgagcggcg
    29041 aagtgatcgt aaacgatcaa gatattacaa aattaaaaaa caaagaatta cgtacattcc
    29101 gaaagaaaat cggtatgatc ttccagcatt tcaacctgtt atggtcaaga actgtacttg
    29161 aaaatatcca gctgccgctg gaattagctg gcgtaccaaa gagcaaacgt aaagaacggg
    29221 cagaagaact gttgcgttta gtcgggttag aaggacgcgg acaagcatat ccttcacaac
    29281 tttccggcgg acagaaacaa cgcgtcggta tcgcacgagc attggcaaat gatcctgaga
    29341 tcctgctttg tgatgaggct actagcgcat tggacccgca aacaacagaa gaagtattag
    29401 acttattgtt agcgatcaat aagaaattga atttgaccat cgtcttgatc acacatgaaa
    29461 tgaatgtgat ccgcaaaatc tgcaacaaag tcgctgtgat ggagttgggg aaagtcgtag
    29521 aagaaggaga cgtattgact gtcttcagac aaccgaaaaa agaagtaacc aaacgctttg
    29581 tacaacaaga catcgaacca gaagctgatt cgacagaact attagctgaa ctcatcaaag
    29641 aaaatccaag cggccgtttg gcaactttga cctttagcga aagaaatgcc aacgagccag
    29701 tcatttccca agcgattcga cgctataaca tcgatgtcaa cgttgtgtat ggaaagatca
    29761 aacaagtgaa agaaggttcc tttggttcat tgactgtctt gatgactggt gaaaccaaag
    29821 aattagatca agcagaagtg ttcatcaaag aacaaggagt aggaattgag gtgatccatc
    29881 gtggataaaa gcttttcaga aacatatcta gattttaaac aaatcaaccc ggaaacgatg
    29941 aatcaggcta tccttgatac actatatatg acattaatct caatggtctt agtgacgatc
    30001 atcggattag ttttgggatt gatcctttac tctattggac gcaaaaacaa accgtatgct
    30061 cgaatcattt atggaatcgt atcgatcatc agtaatattt tccggtcaac gccatttatg
    30121 atcctgatgg ttttgatcat tccatttact aaagcaatcg taggcactat gctaggtgcg
    30181 caagcagcca ttccagctct tgttttatcc gcagcaccat tttatgcccg tctagtagag
    30241 attgcttttc gtgaagtcag ccctggtgta ctggaagcag cagatgcaat gggagcaagt
    30301 tactgggaaa tcatctggaa ggtcttgata cctgaaagta ttcccgcatt gatttctgga
    30361 ttaacagtta caacgatctc gatgatcgga tttactgcga tggccggcgc gatcggcgca
    30421 ggtggacttg gaggtcttgc ttggcaggaa ggctaccagc gtggtaatct aacagtgaca
    30481 ttcgttgcga ctttaattat tttactaatc gtatttatcg ttcaaggaat cggtgatttc
    30541 ttaactaaga aaacagacaa aagataatag gatatagggg gatgtaggat atgaaaaaga
    30601 aaatttttgg atttgcagca gcgcttttgc tgactgtagg attagcagca tgcggtaaca
    30661 acagcgacag caaagattca gcaaataaag cagacgacac gacattgaaa gtcggcgctt
    30721 caccgacacc gcacgcagag attttggaac atgtgaaacc attattgaaa gatgaaggaa
    30781 tcgatcttga aatcgtcaaa ttcgatgact atgtattacc gaaccaatct ttggaagaag
    30841 gcgacattga tgccaattac ttccagcata ttccttattt gaacaaagaa atcaaagaaa
    30901 aaggatatga ctttgtcaat gccggtgctg tccacatcga accaatgggc ttgtactcta
    30961 aaaaagtcaa agatgtttcc gaattaaaag atggtgcaac aatcattact tcaaattctg
    31021 aatcagactg gggacgcatc atcacaattc tacaagatgc tggcttagtc aaagtcaaag
    31081 atggagtaga tctggaaaca gcaacattcg atgatattga agaaaaccct aagaacttga
    31141 agttcaacca tacgatcgac ccatcacttt tagcttctac gtaccaaaat gatgaaggtg
    31201 acctagttgc aatcaacgca aacttcgctt acggcgcagg gttgaatcca ttagaagatg
    31261 ccgtattgct tgaaaaggac aactctcctt acgtgaatat tattgcgact cgtaaaggtg
    31321 atgaagacag tgacaaaatc aaaaaactga ttgaagtgct tcaccgcaaa gaagtccaag
    31381 aatggatcac agacaaatgg ggcggctctg ttaaaccagt aaaggctgat gcaaaataag
    31441 gtatggaaca aaaaagatga gaaataagtc tgtaatggct tatttctcat cttttttata
    31501 cctattctaa tttagaaatc aaaattgttt aataataaat aaaagatatt tgattttagt
    31561 aaggaaggtc attgcgcttc attctcaatt agtctaaaat gagtgagaac gaaagagaat
    31621 ttgggggtat aatgcatgtc tgtcttagaa ataaaaaatc tacatgtgtc gatcgaggaa
    31681 aaagaaatcc ttaaaggcgt aaacctaaca atgaaaaccg gagaaatcca tgccatcatg
    31741 ggacctaacg gcacaggaaa atctacctta tctgccgcta tcatgggcaa tccaaactat
    31801 gaagtaacag aaggcgaaat tttgtttgac gggaaaaatg ttttagactt ggaagtagat
    31861 gaacgtgcgc gacttggctt atttttagct atgcaatatc caagtgagat accaggtatc
    31921 acgaatgcgg aattcatgcg tgcagcgatc aatgcgaaac gtgatgaaaa cgacaaaatt
    31981 tctgttatgg catttttgaa aaagttagat gaaaaaatgg ctttactaaa tatgccagaa
    32041 gaaatggctg aacgttatct gaacgaaggt ttttctggcg gagaaaagaa acggaatgag
    32101 atcctgcaat tgttgatgtt agaacctaca tttgcaattt tagacgaaat cgattcaggt
    32161 ctggatatcg atgctttgaa agtcgttgct aaaggagtaa atgaaatgcg cggagacaat
    32221 ttcggtgcat tgatcatcac ccactatcaa cgattattga actatatcac acctgatgtc
    32281 gttcacatca tgatggaagg ccgagtagtc atgacaggga atgctgacct agctaaacgt
    32341 ctggaagcag aaggctacgc tggtatcagt aaagaattag gaatcgacta taaagaagaa
    32401 gaagcgtaag ggggagcaag aatgaaagaa aaaaactggc tcgattatct tgacgcggta
    32461 aatgattttt ctttaagtaa aggcgaacct gattggatga gaaccttccg tcaagacgca
    32521 ctagcgaaag cagatgaact gccattgccg catatcgatc gggtgaaatt ccatcgctgg
    32581 tcgttatttg atgtgaaaga aacacaaacc atcagtgaaa caggcacgat tccggcattt
    32641 gatgcaatga aagacaatcc tgtgcttgtc caacaaggtt catggacgat ttttgaacaa
    32701 ctgcctgtgg aattagcaga aaaaggagtc atttttaccg acttgttcac tgcaatgatc
    32761 gaatatccag aactagtcca agaatattat atgaaaaaag cagtaaacat gaacgaagac
    32821 caattgactg cactccatgt agcttttatg aacagcggta ttttcctgta tgtgcctaaa
    32881 aatgtcgtga tcgatgaacc actcgagtcc ttattcattc aagatggcgc atccgatgag
    32941 cacttcttca agcatgtgtt gatcgtggca gatgaacata gtgaattttc ttatctggaa
    33001 cgtttccaga caaccaaaga acaagtagca aaatcatcag gtaatatcat cgtggaagtc
    33061 atcgctaaag ctggttcaaa aatcaaatat tcagccgtag atcaattagg agaaaatatt
    33121 acttcttata tgaatcgccg tgggcacatt ttacgagatg cttctgtcga ctgggcaatt
    33181 ggtgtcatga atgacggtca tgtgatcgct gactttgact cagatttagc aggagaaggt
    33241 gcacacgcag aagtcaaaat cgtggcaatc agctcgggca gacaaatcca aggaatcgat
    33301 acacgagtaa ccaataaagc ccctcatacg atcgggcata ttttgcaaca cggagtgatc
    33361 cgagaaaaag gaacattgac ctttaatggt atcggccaca tcttgaaagg ggcaaaaggt
    33421 gcagatgccc aacaagaaag tcgtgtcttg atgctttctg ataaagcacg tggtgatgcg
    33481 aatcctattc ttttgatcga tgaaaacgaa gtaactgctg gacatgctgc cagtgtagga
    33541 cgagttgatc cagaagaaat gtactatctg atgagtagag gattgcataa agaagaagca
    33601 gagcgcttag tgattcgagg attcctcggt tctgtcttaa cagcaatccc tgtagaacaa
    33661 gttcggaaag aattagtcga agtaatcgaa gggaagttga atggatgatc gatgctaaaa
    33721 agctacgcaa tgattttcct atcttatttc aagacgtcaa tgatgaacct ttagtttatc
    33781 tggataatgc agcgacgacc caaaagccaa aacaagtact ccaggcatta gcgtactatt
    33841 acgaacacga taacgccaat gttcatcgcg gtgtccatac gttagcagaa cgcgcgaccc
    33901 atgattatga acaagcaaga gaaaaagtac gggcgttcat ccatgcaaaa gaaacagcag
    33961 aagtgctgtt tactcgtggt acgacgacaa gcttgaattg ggtagccaga accttcggcg
    34021 accgctatat caaagcagga gacgagatag tgctctctta catggaacat cattctaatg
    34081 ttatcccgtg gcagcagttg gcaaaacgaa caggtgctgt cttgaaatat atcgaactaa
    34141 cagaagaagg cttcctggat atggaggatg cagcgaaaaa gattacggac aaaaccaaaa
    34201 tcgtctccat tgctcatgta tccaatgtct taggtgtctt caatcctgtt caagaattag
    34261 ctgaacttgt ccatgaaaac ggcggggtca tcgtagtaga tggtgctcaa tctgccccgc
    34321 acatggccat tgatgtccaa gcactagact gtgatttctt tgctttcagc ggacacaaaa
    34381 tgtgcggacc aacaggtatt ggtgttttat atggtaaacg aaaatggtta gaagaaatgg
    34441 aacctgttga atttggtggc gaaatgatcg attttgtcga attatacgac agtacatgga
    34501 aagaactacc ttggaaattc gaagcaggga caccaaatat cgccggcgct atcgcattag
    34561 gccatgcaat cgattatttg caaaaactcg gtatggacaa tatccatcga tatgaagaag
    34621 aactagccgc ttatgtgttg ccaaaattac aagcaattga tggattgact gtttatggtc
    34681 cacaagaccc gaattttcgg acaggagtcg ttgcattcaa tttggatggg ttacatcccc
    34741 atgatgtcgc gacagcttta gacatggaag gggtagccgt cagagcaggt catcattgtg
    34801 cgcaaccgct attgaagtac cttgaagtat cagcgactgc tcgcgcgagc ttttatttct
    34861 acaacacaac gcaagacgcc gaccgtttag ttgaagcaat catggcaaca aaggagtttt
    34921 tccaacatgg gactatctaa attagacaat ttataccgtc aagtgatctt ggatcattct
    34981 tcccatccgc atcatcgtgg aagcttgact gactcgacaa gcaagatcga attgaacaac
    35041 cccacatgtg gagatgtgat cgaattgcaa gtcgatgtaa aagacggaat catcaaaaat
    35101 atcgcctttg acggcagtgg ctgctccatc agtacggcaa gtgcctcgat gatgacagac
    35161 gccgtcatcg gaaaatcaat agaagaagca gaacatttaa ctgtagactt tctacagctt
    35221 gtccaaggaa aagaagtcaa agatcccgac caattaggcg atgctgctat gcttggtggt
    35281 gtagctaaat ttcccgctcg gatcaaatgt gcgacacttg cctggaaagc actaggaaaa
    35341 gcaatggaaa acgatggtac cgcagctttc catcattacc acaaggaaga ggagtgagaa
    35401 catgggcgta ccagaattag aagaatataa attcgggttt cacgatgatg tcgaaccggt
    35461 tttcagtaca ggagaaggct tgacagaaga agtgatccgt gagatgtcaa gaatcaaagg
    35521 cgaaccagaa tggatgcttg aattccgtct gaaatctcta gaaacattta acaaaatgcc
    35581 aatgcaaaca tggggccccg acctttcaga catcgatttc gatgccatca aatattacca
    35641 aaaaccaagt gacaaacccg ctcgtgactg ggaagacgta ccagaaaaaa tcaaagatac
    35701 atttgaaaag attggtatac cagaagctga acgagcttat ctagccggtg cttccgcaca
    35761 atatgaatca gaagtcgtgt accataatat gaaagatgaa ttcgaaaagt taggaatcgt
    35821 ctttactgac actgactctg ctttgaaaga ataccctgaa ttattcaaag agtacttttc
    35881 aaaacttgta ccgccaaccg acaacaaatt agcagcgctg aattcagccg tatggtcagg
    35941 tgggaccttt atttacgtgc caaaaggcgt caaagtcgat gttccattac aaacgtactt
    36001 ccggatcaat gcggaaaaca cggggcaatt cgaacgtacg ctgatcattg ttgacgaagg
    36061 agcaagcatc cactatgtcg aaggttgtac ggcacctact tattcaagta acagcctgca
    36121 tgccgccatc gtagaaatct ttacaagaaa agatgcgtat tgccgttaca caacgatcca
    36181 aaactggtca gacaatgtgt acaacttagt aaccaaacga gcaaaagcat acgaaggcgc
    36241 aaccgttgaa tggatcgacg gaaacctagg tgcaaaaaca acgatgaaat acccaagtgt
    36301 ttacttagat ggaaaaggtg cacgaggcac catgctctcc atcgcctttg caggagcaaa
    36361 ccaaatccaa gatacgggtg caaaaatgat ccacaatgca ccaaatacat ctagctctat
    36421 cgtctctaaa tcaatttcaa aagacggcgg agaagtaaac tatcgaggac aagtaacctt
    36481 tgccaaaaac agtgcgggtt cgatctctca tatcgaatgt gacacgatca tcatggacga
    36541 atggtcgaaa tccgatacga tcccattcaa tgagatccac aacagccaag tctctttaga
    36601 acacgaagcc aaagtctcaa aaatctcaga agaacaactc tactatctga tgagccgagg
    36661 cctgaccgaa tctgaagcaa ctgaaatgat cgtcatgggc tttgttgaac cattcaccaa
    36721 agaacttcca atggaatatg cagtcgaact caatcgattg atcagttatg agatggaagg
    36781 cagtgttggg taaacctgtt ataggagcta gaacccttga tatataaggg ttctagcttt
    36841 tttgttttgc ctgtatacta gaaatgacca gattttgacc agctctttta ctgatgagcc
    36901 atcatatcag caaagtcttg acctgttttc ttaggtgcat tatttgtaat atgtgcatag
    36961 atgtctaatg tcactttact attttcatgc cctaatcgtt gttgaactac cttgacagat
    37021 tctcctgctt ctaataataa actagcatgt gtatgtctaa acccatgagg tgtaatacga
    37081 ggcaagttat attttttcaa aatatatctt aaccaatcat taacagtgtt tggaatgtat
    37141 agagtgtttt taactgttgt aaaaacatgt tgctctttag agctagtatt gtatccaagt
    37201 tttaaatact cttctttttg ctgtaatttc cagttgttga ggatagaaag agtctcagta
    37261 tctaaagaga taactcgtct agaagctctt gttttcggag attgaattat aactttacca
    37321 aattcatcta cagcaagagt tttattgaca tgtagttctt tattaaacgt gtctatgtct
    37381 ttccactgta aagcgagtac ttcactcttt ctcatacctg tgtatgccaa taatctgaag
    37441 aaggcgtatt gtttcatgtt accaaaatct ttaaaagcat ccagtaaatt attaagctcc
    37501 tcttttgagt aaaatttacg atttttctca tactcttttt ttcgtggaag aagtgttttt
    37561 tccataggat tattgtcaat atattgcatt gcaaccccgt agcgtaaaat ttgtgcagtc
    37621 tgttttctca tataatcgta actttcatat ttactgtgcc attcatctac cactttttga
    37681 caatatggga cagagatatt cgatagttta agcttaccaa atgctggaag tatttgatta
    37741 tttgcgtatc ttactgagat tgcgattgtt gaagctttaa cactggtacg ctgatgagtt
    37801 atccattcat tatacaggtc ttcaaatgtc atatcaggtg ctttcttttt aacaccgtat
    37861 aatatttgat gtctgtactc gtcataatct ttttgagctt cttgtttgct actaaaacct
    37921 cttctcgtag tatttatttt ttcaccagtt cgtggatcgg tggcaatgtg tcctttatac
    37981 atccacgcca cttgaccgtt tttctttttg tactttgata tttttgccat taaatcatct
    38041 cctcttagtt tacaaggagt acttaccact agatggtatc acctcccttc atttttttct
    38101 tagtgtattt tttcccaatt gatgaaatcg tttgttctag cttgtttata ttatcagtaa
    38161 tttctctcac agcttcttcg ggataagaca tgtctttcaa ttgaataggt agccattcat
    38221 ctaccattct ttctagtagt tcttctatac tgctagtgag gaaaagaatg ttattgtcaa
    38281 attcttctac aatagcattg atgaataatt cttctatttt taagatattg aaaagactaa
    38341 ttttttcatt tttaacttta ttacaagtct tgttagcaat aaattcttgc atctctggtt
    38401 ctaatttgga atatctatcc agccatgtat ctaaattaga gtgtactggt gggaactgtt
    38461 gacgaagatg ctggaacagg tttttatctg gaaatgttat accatctagt acaatgctat
    38521 ctctataaag aataattccc aaaatatatt cttctacatt gccacaaact aactcctcta
    38581 tactcatatt tcccaaatca gcaattcggg ataatcgctc tggtattggt aaataggttc
    38641 ccttctccca ttcgtagata gtttttttgt caattaattg accgttgtta atcataagac
    38701 caaatgctgg ttgtgttaat tttttactct ttcttatagc ttttattttt gttccgagta
    38761 tcttttttcg ttctaaataa tttattggtg gacgttttat catattgacc acacctccta
    38821 aaattagtat acctcaaaaa aggggtgttg taaataaatt ttacaaagct gttgacaccc
    38881 cttttttatc gaggtattat gtgtatgtcg atgaactggt aatcattttt tgtttgatta
    38941 atcggttaat aacaaaatac ttttattttg agcaaaaaat acgaagataa ttcttagaaa
    39001 atttgttcaa gcaatcacga aaggaggggg aaatatgtct ctacaaatca ttgatagcat
    39061 tgtttctata caagttgaac aacaagtgaa agagataatt acagtgctaa agcctatgct
    39121 agaagacatt atttctggag tcactctaga gtatatggac tatcagcagg caagtgaata
    39181 tctaggtgta tcagtaggaa cgattagaaa atatgtttct caatacgggt tacctgttat
    39241 aaaaattgat acggtcatta gatttaaaaa atctgatatt gatagtttca tggaacggta
    39301 taaaggttga gtaaacaaat aagactaaaa catatagtaa taaagagttt gtttttatta
    39361 tctgtatgtt ttagtcttta acagaatttt ataaggagga aacacaaatg gaaacatcaa
    39421 tgattaatat tccaatttat gtagatcaag aaaaattatt agagaagcca gttacaaaaa
    39481 aagaaaaaac tgatgtaaga gataggtgtt tagataacta tagagaagtt acaagagatg
    39541 agtttgtaga gatactaaat tcatcacaat catttattcc aagcaagaca aagaataaag
    39601 gcaatgacaa ggagagtttt gtggaaacca gagtaattat tctggatgtc gataacactg
    39661 ttaaagatga gaaggggaag gtagtagatt tgagtaaaga tgatagtaga tacttaagca
    39721 tagaaaaggt tttatctatc aatttagttc ataattctgc gtttgcgata caaaaaagta
    39781 ttcggtactc tgaaaatcta gaaaaataca agattgtctt tctattaaaa gaagcaatta
    39841 ctgactatgg taaaatggtt gctgtttacg aatacttgaa aaagaacatc cccgggtgtg
    39901 atgacaatgt aaatgcttcg aatagaatgt tttttggagg atataagtca gatgcagtga
    39961 taataattaa tgaagacaac atgttagata ttactgagct tccgattgat tttaccatga
    40021 catgttctaa aaattgtcaa agtgattcag ggagagagta tgaactagtc aatgaaaccg
    40081 attttgttaa gttgataaaa aatgacgaca aagaagaaat gaaacagtgg tttaaagact
    40141 gcctgtttga ttcctccgac atgtctttta atgagattta tgaaagattg ttaaccattg
    40201 atatgaataa attacttaag tcaagtaaaa acttaagatg tctgtttcac aacgatcata
    40261 atccctctgc tagtattttt accagtaaaa ctgggcattc cattttttat tgtcattcca
    40321 gtaattgtga tatcagtgaa aattttattg gagttgtgat gctaatttta aagaaagaca
    40381 gtcgggtaga aacatttaat tggctattag agaacctaga cttgtatcct acacattttc
    40441 gaaaattgaa agaggaatca aaagtacttt ttgacactct tgaatcaatg aaagacaaac
    40501 ttttcaggac aataaggaat tatctagagg aaatgcgacg tgtctatgat gtcttgcttt
    40561 ctgatgttaa tttttttgat gatagtaagg aaagtgtgac ttgtattttg tcgggggaac
    40621 aattagctaa aaaaatgagt tcttactatg gtaaaactta tgacatagat aaatgtaata
    40681 agttactatc acttatgact tttataggat tgataaaaaa attagatgat gaagatgttc
    40741 ctttgaagat gctcgaatac ctaaataggt taaaggaaga gaaagcgtta ggaaacttta
    40801 atcataagag aagcaatgtc tatcgattag acgtttttaa tgcaacttct gtgattcaaa
    40861 aattgaatga agatatcctt ccacttctgg aaaaaattca ttttacatat caatattttt
    40921 cttatgaatg ggttaagata tgttttaacg aaaaagaggc taaaagagta tttcctcaaa
    40981 attcccgatc agaactcagc aaggaaaaac aattgatttt agatgaagca catgaagttt
    41041 tacagggctt atcgtggcaa gggacatatc taatgacaga gaaagaactt attaacactg
    41101 tttgtgaacg cctacgtaat gttggggata acagcgtgaa aaatgcttta ataaaaaaca
    41161 gaggctactt tgttaaacaa ggattcactc ttgaacgagc ttctaaggat attaagaaac
    41221 tatacaatgt gaaagcaagt agtagttttt taatatattt agcgaaaggg gcagggatag
    41281 atgaaacaat aaaagaattg atattatcag gagctgggat agttcctctg tatcaattta
    41341 caagtgtaac aagagatgat ggacggagag taaggaagag aaaagaccca attgtattaa
    41401 actagacagg ttatccatag ctcttaatct tatttcatct caatacaccc accgttaaag
    41461 ttcggtgggt tatttatttt tcaatctttc aatagctgat tgcgttcatt ttcttgcctt
    41521 tttaatagct tcatatagtc aattgttcga actgcatctt cagtgctaag agaacgatag
    41581 agagccaaaa actcttcctc ataatctacg acaacattgt ctagaaaatg gcttacaggg
    41641 gttcctaggg ctctagaaag agctgttaag ctatcgaggt taggctctgt tacagaattt
    41701 tcccatcttg ctactgcctg ttgtgtagag aagatttttt cagctaattc tttttgtgtc
    41761 cacccgagag cttttcgctt ttctctaatg acctttgcat attgtttctg tttcattaat
    41821 acacccccat ctcagtatag tacaacaagt tgttgtattt gtaatggtat ttgaagtttt
    41881 ttatatttta tgagttgaaa tacatcatat tgttgtatat aataaaacag gaggggagaa
    41941 tcatgaaaaa agtaagtaat ctcagccgat acagaaaaga aaaaggactt tctcaaacgg
    42001 agttagcaaa aaaaatgaat gttacacagc aatgtgtaag ttcgtggcaa acagggagaa
    42061 cgattcctaa accctatcaa atgaagatgc tttctgagat attaagtgta ccaatgaatg
    42121 agctgttttc tgatgtattt aataaagtaa acagttaact aataagcaat tgaatgattt
    42181 taggaggaga agacatgtat tgtagaaaat gtggcaaaga aataacagat gaaagtaagt
    42241 attgccagtt ttgtggagca ctacaagaaa cagatgagac aaccggaaca gacacagtaa
    42301 agaaaagtac tgagcagaca attaatgaaa tgaaggaaca aatcgtacat ggacaagaga
    42361 cagggaagct gtatttgatt attggttgga tttctatggt agtatcgtta gcatttattc
    42421 ctgttgtatt tggtgctgtt gctgttatta tgggctactt atatcgagaa aaagatgaga
    42481 aaatgggtac gattttgatg atagcagggg tagctggagc aatctttggt gtacttttag
    42541 ggatgtcaat gtactgataa gagggataag aacaaattat tttggagata ggagataaaa
    42601 agttgaataa agtaaatatg aacaagaagt ttgtcattat aggtattgtt gtcttggcta
    42661 ttataggtat tattttttca aaaatgacag gtacatctgg attagagaag tcatcaaaac
    42721 cattggtaac acagatttta caagaacagt acaacttaaa tcgatcatgt gatgatgtga
    42781 cgattactca tgaaaatgga gataacacat accgggctaa agctatatta gacaatggaa
    42841 gtgctatcaa cataaatatt gagtactatc caaagaaaga ccgtatatat gtggagatac
    42901 catatgctga ggtgttaatg ttaaattgat ttaaatgtat tatgcatacc gtccaaacag
    42961 gaactcccaa aggtgtaaat tcactaggga gtttttatag tttcctataa attaaatact
    43021 aatacaggtg acaaaaaaat attacaatga ttgtcgggaa tttttatgtt attcactgtt
    43081 ataacaatat aaaagaaatg aaaaagaaaa aattattatg gatatgtttg gtatttttat
    43141 tgataattgg tggaattgaa atacttacta catgtagatt agaaataaat cagtttaaac
    43201 atttgctagt
//
LOCUS       DAFBQH010000015        42486 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.015, whole genome shotgun sequence.
ACCESSION   DAFBQH010000015 DAFBQH010000000
VERSION     DAFBQH010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.015"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            75..521
                     /locus_tag="LK694_000889"
     CDS             75..521
                     /locus_tag="LK694_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5051778.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(580..1041)
                     /gene="mscL"
                     /locus_tag="LK694_000890"
     CDS             complement(580..1041)
                     /gene="mscL"
                     /locus_tag="LK694_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBK5051779.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            1293..2561
                     /locus_tag="LK694_000891"
     CDS             1293..2561
                     /locus_tag="LK694_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBK5051780.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2554..3840
                     /locus_tag="LK694_000892"
     CDS             2554..3840
                     /locus_tag="LK694_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBK5051781.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            4579..5502
                     /locus_tag="LK694_000893"
     CDS             4579..5502
                     /locus_tag="LK694_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5051782.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            5711..6289
                     /gene="pgsA"
                     /locus_tag="LK694_000894"
     CDS             5711..6289
                     /gene="pgsA"
                     /locus_tag="LK694_000894"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBK5051783.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            6529..7770
                     /locus_tag="LK694_000895"
     CDS             6529..7770
                     /locus_tag="LK694_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBK5051784.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            7879..8928
                     /gene="recA"
                     /locus_tag="LK694_000896"
     CDS             7879..8928
                     /gene="recA"
                     /locus_tag="LK694_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBK5051785.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            9339..10895
                     /gene="rny"
                     /locus_tag="LK694_000897"
     CDS             9339..10895
                     /gene="rny"
                     /locus_tag="LK694_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBK5051786.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(10974..11780)
                     /gene="proC"
                     /locus_tag="LK694_000898"
     CDS             complement(10974..11780)
                     /gene="proC"
                     /locus_tag="LK694_000898"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBK5051787.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            11921..14494
                     /gene="mprF"
                     /locus_tag="LK694_000899"
     CDS             11921..14494
                     /gene="mprF"
                     /locus_tag="LK694_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBK5051788.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            14636..15499
                     /locus_tag="LK694_000900"
     CDS             14636..15499
                     /locus_tag="LK694_000900"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBK5051789.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            15744..16682
                     /locus_tag="LK694_000901"
     CDS             15744..16682
                     /locus_tag="LK694_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBK5051790.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            16705..17385
                     /locus_tag="LK694_000902"
     CDS             16705..17385
                     /locus_tag="LK694_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBK5051791.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            17358..18182
                     /locus_tag="LK694_000903"
     CDS             17358..18182
                     /locus_tag="LK694_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBK5051792.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            18349..19167
                     /gene="purR"
                     /locus_tag="LK694_000904"
     CDS             18349..19167
                     /gene="purR"
                     /locus_tag="LK694_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBK5051793.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            19670..21043
                     /gene="glmU"
                     /locus_tag="LK694_000905"
     CDS             19670..21043
                     /gene="glmU"
                     /locus_tag="LK694_000905"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBK5051794.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            21137..22108
                     /locus_tag="LK694_000906"
     CDS             21137..22108
                     /locus_tag="LK694_000906"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBK5051795.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      22114..22216
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22282..22851
                     /gene="thiT"
                     /locus_tag="LK694_000907"
     CDS             22282..22851
                     /gene="thiT"
                     /locus_tag="LK694_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBK5051796.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(22932..23705)
                     /locus_tag="LK694_000908"
     CDS             complement(22932..23705)
                     /locus_tag="LK694_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051797.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            23971..25671
                     /locus_tag="LK694_000909"
     CDS             23971..25671
                     /locus_tag="LK694_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBK5051798.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            25641..25859
                     /locus_tag="LK694_000910"
     CDS             25641..25859
                     /locus_tag="LK694_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HBK5051799.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            25879..26769
                     /locus_tag="LK694_000911"
     CDS             25879..26769
                     /locus_tag="LK694_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBK5051800.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            26787..27071
                     /locus_tag="LK694_000912"
     CDS             26787..27071
                     /locus_tag="LK694_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051801.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            27558..28172
                     /gene="gmk"
                     /locus_tag="LK694_000913"
     CDS             27558..28172
                     /gene="gmk"
                     /locus_tag="LK694_000913"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBK5051802.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            28177..28482
                     /locus_tag="LK694_000914"
     CDS             28177..28482
                     /locus_tag="LK694_000914"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBK5051803.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            28735..31143
                     /gene="priA"
                     /locus_tag="LK694_000915"
     CDS             28735..31143
                     /gene="priA"
                     /locus_tag="LK694_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBK5051804.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            31158..31649
                     /gene="def"
                     /locus_tag="LK694_000916"
     CDS             31158..31649
                     /gene="def"
                     /locus_tag="LK694_000916"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBK5051805.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            31642..32580
                     /locus_tag="LK694_000917"
     CDS             31642..32580
                     /locus_tag="LK694_000917"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBK5051806.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            32580..33938
                     /gene="rsmB"
                     /locus_tag="LK694_000918"
     CDS             32580..33938
                     /gene="rsmB"
                     /locus_tag="LK694_000918"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBK5051807.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            33951..34691
                     /locus_tag="LK694_000919"
     CDS             33951..34691
                     /locus_tag="LK694_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBK5051808.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            34688..36757
                     /gene="pknB"
                     /locus_tag="LK694_000920"
     CDS             34688..36757
                     /gene="pknB"
                     /locus_tag="LK694_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBK5051809.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            36922..37821
                     /gene="rsgA"
                     /locus_tag="LK694_000921"
     CDS             36922..37821
                     /gene="rsgA"
                     /locus_tag="LK694_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBK5051810.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            37834..38484
                     /locus_tag="LK694_000922"
     CDS             37834..38484
                     /locus_tag="LK694_000922"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBK5051811.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            38485..39123
                     /locus_tag="LK694_000923"
     CDS             38485..39123
                     /locus_tag="LK694_000923"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBK5051812.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            39278..40132
                     /gene="mreC"
                     /locus_tag="LK694_000924"
     CDS             39278..40132
                     /gene="mreC"
                     /locus_tag="LK694_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBK5051813.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            40136..40645
                     /gene="mreD"
                     /locus_tag="LK694_000925"
     CDS             40136..40645
                     /gene="mreD"
                     /locus_tag="LK694_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBK5051814.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            40900..42438
                     /locus_tag="LK694_000926"
     CDS             40900..42438
                     /locus_tag="LK694_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBK5051815.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTVPESSATEE
                     SKTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVTPAPAP
                     SGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWTVPQES
                     AGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQWFAPDF
                     AVSM"
BASE COUNT        14005 a         7582 c         9187 g        11712 t
ORIGIN      
        1 attcgtaagg ttcgtttgct tttttgtgtg cgaatcggta tgctaagaat aaaaaagcag
       61 ggggcgtttg cttagtggaa tatcaggtaa agaaatttga agaattgtca acagcagagt
      121 tttacgcgat cgtcaaagaa cgaatcgcag tttttgttgt ggaacaacag tgtccttatc
      181 aagaagttga tgacattgat ggacaagctt ggcatacgtt ttttcaagat actgatggga
      241 agatccttgc ctacacgcga gtatatgaag aagcggagag tatccatttt ggacgtgtcc
      301 ttgttcatca ggacaaccgt aaagatggtt taggaagaaa gattgttcaa gaaacgatta
      361 acatgatcca gaaaaatttt ccagaaaaac caatcgtgat cggcgcacaa gaatacctac
      421 aagcttttta tgagtcgttt ggattcaagg caatctcaga tgtgtatttg gaagatgata
      481 ttccacatat cgacatgaaa aaggaacaag tagcaagtta agaatctaaa aaaagaaagc
      541 cgacactgaa aatcggcttt ctttttttta gattctttat tatttagaag tatcggaaag
      601 atttgtttgc tctactggat gtgtattgtc ttctgccact tgtttggcaa gaagatcgcg
      661 tatgtcacta agataatctt ctgcagtagg agtttcttcc acttccactt gctcttcttt
      721 ggagcgtaaa ttcttcgcac ggtttgccgc tttgacaact aagaataaga caaaaccagt
      781 gatcagaaat gtaatgatag cactaataac gttaccaaac gcaaatgtga cgccttttgt
      841 cggcgaccag tcaagaattg aaagtgctcc ttctaaatca gaagtacctg tgatcattgc
      901 gacaaaccaa cctattagtg gtgtgatcaa gccttgcacc acttggttta cgatgcttgt
      961 aaacgcacta ccaataacaa cgccgacagc aagatccaag acatctccac gcatgatgaa
     1021 ctctttgaat tctttgatca tgaatgaccc tcccttcttt tatttgtctt tagtatatcg
     1081 aaaaataaaa aataaaaaaa caatatacgc ttttagccgt ctgtttcttt tgataaaaaa
     1141 catctatgct atactataaa gtgtaacatc atttagaaaa aacggcgaat tgcctgaact
     1201 tataaatttc aagcagagga ataagtaaaa aaggcaggtt gacccattaa aagagtaagc
     1261 gggcaatcaa ctgtgaaaag gagcatataa atatggtgta tcaattggca gaaggcgtga
     1321 atcttcatgt cttaccaaca aaacaataca aaacgatccg gatttttatc cgttttactg
     1381 cgcgtctgca acaagaagtc atcacgaaac gttcacttct cagcagcatg ttggaaacaa
     1441 atagtttgaa ttatccggat cagacaaagt taagtgcgca acttgcagag ctttatggcg
     1501 caagctttgg attatcagtt agaaaaaaag gaaatttaca ttggctaaac gcagggatca
     1561 gttttgtgaa tggtgaatat gtcaatgatc caaacctttt ttctcaagcg gtagattttt
     1621 taaaagaggt tttattttat ccaaatatta aaaatcatca gtttgatcag ttgacgtttg
     1681 acttggaaaa gaacaatctg cgtctatatt tggaaagttt gaaggaagat aaacaaactt
     1741 ttgcttccta tgccttgcaa gaactttatt ttgaaaacag cccggaacaa aagatcccag
     1801 ggttgggcgt agtcgaagat ttggacaaga tcactgctag ttcgctagcg tcctactatc
     1861 aagaaatgat ggcaaatgat caaatcgaca tttttgttgt cggcgatgtt gatccggaca
     1921 aagcagctga agcagttggg caattaccat ttgagtcgcg ggaaacagtt tatccagatc
     1981 tgttttatac ccaacctcaa gtgaatatcg tgaaggaacg acaagtacgt gagccaatcg
     2041 ttcaagcaaa gctgaaccta gcttatcaga caaatgtgta ttacgatgaa ccagaacgtt
     2101 ttgcgctaat ggtctttaac ggtttatttg gcggttttcc tcactctaaa cttttcatga
     2161 atgtcagaga gaaagaaagt ctagcttatt atgcctctag tagcgtggat acgttccgag
     2221 gattcatgag tgtccaaacc ggtatcgatg aaaagaaccg aaatcaagta cttcatctga
     2281 ttcacgagca attagaaagt ttgcgaaatg gtgaaataac agagttggaa ctgtctcaga
     2341 caaaagcaat gctgagaaac caatatctgc tttctctaga ctctccgcaa gcggcaatcg
     2401 aagcttcttt tttggacagc tggctgccgg aaacaaaatt atctgatgaa gaatggttga
     2461 aacgtatgga gagcgtaacg atcaaagaga tacaacaagt agctgagcaa atcgaattgc
     2521 aggcaatctt ctttttagca ggaggaaatg caaatgaata aaacagaata tgagcaaatc
     2581 aatgaaacac tttatcatga agtactgcca aacggtttga ccgtgtattt gttgcctaaa
     2641 aatgactatc acaagacata tggtttgttc agtacgaatt atggttcgat cgataacgaa
     2701 tttattccct atggtgaaaa agaaaaggtg aaagttccag atggaattgc ccatttttta
     2761 gaacataagt tgtttgaaaa agaagatggt gatgtctttc aattatttgg gaaacaagga
     2821 gcttccgcca atgctttcac aagttttacg aaaaccagct atctgttttc tacgacggat
     2881 caggtagaaa agaatctgac gactctgatc gattttgtac aagcacctta cttcacagaa
     2941 gaaactgtaa acaaggaaaa aggaatcatc ggtcaggaaa tccaaatgta tgaagacgac
     3001 cctaactggc ggatgttttt tggcatacta aataacttgt atcctacaca cccattgcat
     3061 atcgatattg cgggcacggt tgaaagtatc gacaaaatca cagctcaaga cttgtatacg
     3121 tgctatcgga cgttttatca gccaagcaat atggttttat ttgtagttgg taagatggag
     3181 cctgagaaat tgatgaaact tattcgggaa aaccaagaag caaaaaattt ccctccaaag
     3241 caagaaattg tacgttattt ccctgaaaac acaaaagaaa tcatcaaaca aagtgcattg
     3301 gaagcagcca ttacacgaga taaatttgta cttggtatta aaggattaga cacattgccg
     3361 caagaaggaa cagaactgtt acgctataaa acagcaatca atctgttgtt ccaaatgatt
     3421 ttaggcaata cttctcgaaa ctatctagcg atgtacaatc aaggaattat cgatgacagc
     3481 tttggttttg aattcagctt ggatcgtgaa tttcactttg cggacttcag cggagatacg
     3541 gacgaaccag agaaagcagc agaaaaagtc aaagaaatca ttcttggatt cgctgatgat
     3601 ccggaagtgt cagaaacaaa tctcgatttg ctgaagaaga aaatgcttgg acaatatttc
     3661 caatcattaa attcgatcga atatattgcc aatcaattta cgcaaagttt atttggcgat
     3721 cgaactttat ttgatcttcc tgaaatcatc gattcgatcc aaatgaaaga cgtactggca
     3781 gctggggaag catttataag tgaagaggcc ttcagcgagt tttacatgag accaaaataa
     3841 accagtattt tatgaggaaa agtcttaaat ttcgttttag acttgtagta ggaacctcct
     3901 ccaaaatcag cttttatggt attcttagtt gtgttggaaa aattagtgag aaaataaaac
     3961 aaatcatgct ctcttttaat caacttatat taaaataaaa tcaaacgaag taatcaaaga
     4021 ggttgtgaca gaagtggtca gcttcaagaa ataagaagga atttacgaaa attgcttctc
     4081 aaatttttgt gatatttcgg cttattgccg aagaagctac ttctggaaca ccgtgtacca
     4141 agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaagta aaaacagctt
     4201 cttaatgttt ttaacttggt tggacttatt accgcagatg caagcttttg aacaccgttt
     4261 aaggaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacat acggaaaaat
     4321 atctccttat attttgccgg tttttgagct aataccgagg aacagctgat tgaacaccgt
     4381 ttaagaaaga ggtcgtgagc ctgccgttta actccgagtc acaaggaaca agttcaaaaa
     4441 acagcttctt atatttattg gatattgtga cttgtgaccg aggagttggc aggagaacaa
     4501 tgtttattcg gaaaaaagga gctgtgacat gacttttgtc acagcaccca aacataacgt
     4561 ctttcgttgc aacagcagat gcttcggaga atcaaacaga ctggagaaga tgaacgagtg
     4621 gcttttgaaa ccatcggaga aaaattacgg caagcacgcc tgaataaaaa aatatcttta
     4681 gatgaattac agcagattac aaaaattcaa aaacgttatt tggaagcaat cgacaatgat
     4741 gattttgacc aattgcctgg taaattttac gtgcgggcat ttattcggca atacgctgaa
     4801 gctgtaggag aagatggaga ccatttagtc gatgtttttg atgggaaaag caaattgaca
     4861 ggatcggtcc ctgaacgtcc gcagcctgaa acagtaagta cttctagaaa agcaatgcat
     4921 caggaagaat cgcatccaag tcgcttttgg acaagtcttc ctgtcatttt attaggattg
     4981 gttgctttgg cgattgtcgt tgtcgtgggc tatatgactt ggcaagatcg tcaagctgat
     5041 ccaataatcg gagacacagc ttcttcgctt aaagtagaag gatcagtcga ggagtcctct
     5101 tcctcagagc aaagcacgca gaccaaagag tcttctacag tagaatcaac aacaaaatca
     5161 agtgaagaaa agaaaatggc aatagctaca ggagaagatt ctggcagtgc catcgcagta
     5221 aatatcacag acgctaccaa gcctgtaacc cttgaattta ctgctgtcaa tcgtgtatgg
     5281 ttaggtgtgc tagtagacaa tgcctatgtt tatcaaggga cactggcagc aaatgaaaca
     5341 cagagtaccg tgttaccgga aacagcaaca aatgcgacca tcacgattgg tgcagcaagc
     5401 aatgcaacga tcaaagcgaa tggagaatcc gtaccagtta atccagggga aaacaatcag
     5461 agtccaaaaa atgtgaacct tactcttcaa tacgcagaat gatcgtataa attagtttac
     5521 gcatctgttt acttgtaggt ataaaaaaga aggatcgtga caaatctgtg tcgcggtcct
     5581 tcttcgttaa ttcaaaaaga tcaatttcaa taaaaatact cctttgaata aattcatttg
     5641 gaaaagtaaa taatatcggt ggaaacttcg ctgatagtca cgtagaatag ggaagaaagg
     5701 agtcgatagt gtgaatttac caaataaatt aactgtactt agaatttgta tgatcccgat
     5761 tttcattttg gttgtaggtt tacctttaga ttggggacaa tttaacgtag gagacaccac
     5821 actggctgtc acacagctag taggtgcagt tatttttgct ttagctagta tcacagactg
     5881 gttagatgga aaaattgccc gtgcgcgagg ccttgtcact aattttggca agtttgcgga
     5941 cccgttggca gataaaatgc tagttatgac tgcttttatc atgttggttg accaaggaaa
     6001 ggcccctggt tgggtagtag cgatcattgt ctgtcgtgaa ttagccgtca caggcttgcg
     6061 tttactactt gtcgaacatg gagaagtcat ggctgcagct tggccgggaa aagtcaagac
     6121 ggctacacaa atggttgcta ttatcttgct gtttatcaat aatatcccat tcagcctgat
     6181 cggtattcca gtagatcaaa tcatgcttta tgcttgtctg atctttacta tctattcagg
     6241 tgtggattac ttcatcaaaa acagcggtgt gttcaaagga tcaatgtaag tatcgtttag
     6301 tggagaggtt gtgagcctgc cgtctaactc tgagtcacaa gggataattt caaaaaacag
     6361 cttttcatgt tttatgtata ttgtgactta tgaccgaggt tttggcagga gaacactgtt
     6421 tatttggaac aaggagctgt gacaagattt ttgtcacagc tccttgccct ctttttaaat
     6481 aaaaaaatat aaaaatacag cgtatactat ataagggagc gggaagtaat gaaagcagaa
     6541 ataatagctg tcggaacaga acttttacta ggacaagtcg tgaatacgaa tgccactttc
     6601 ttgtcagaac agctagctga tctaggtatc gaagtttatt atcaaacagt tgtaggagat
     6661 aatcagcagc gtttagaaga gttgattgct ctagctgaaa caagaagtga actcatactc
     6721 ttatgtggcg gattaggacc gacagaagat gacctgacaa aagaagcaac tgcggctcac
     6781 ctcggtaagt ctctcataca aaatacggaa ggatacaaaa aattattggc atattttgaa
     6841 acaacccatc gaaaaatgac caaaaacaat ctgcaacagt cccagattat cgaaggcggg
     6901 gttcctctac caaatcgtac agggcttgct ttagggactt tttatcaaac agatacacat
     6961 gcgtatatcc ttcttcccgg tccgcctaat gagttgaaac caatgtttgt ggaacaagta
     7021 cgtcctttac tagaggaacg ttttccttct gaagaaaaac taatttctaa agttttgcgc
     7081 ttttatggga ttggtgaatc gcgactggtc actgagctaa aagatctgat tgagacacag
     7141 atcaatccaa cgatcgcacc atatgccaaa ccaaatgaag tgacattacg actgacagtg
     7201 aaaacaaatg atgttcaggc agggaatcaa gctcttttag cattagaaga aaaaatccaa
     7261 gaacgagtag gggaatattt ttatggatac ggcgatgata attcgctagc aaaagtcgta
     7321 gtggaactac tgaaagaaaa caagcagact gtaacagcag cggaaagttt gacagccggc
     7381 gccttccaag ccgcattagg cgacattgct ggtgtatctg aagtatttcc aggcggattc
     7441 gttacctact cgctccaaac aaaagctggt tttctggaga ttgatccgga gctattagca
     7501 gaatatggaa cagtcagcaa agaatgtgtg gagcagatgg ccattcaagc acgtgagata
     7561 gcaagaacag attacagtgt agctttcacc ggagtggctg gaccggacac gttagaaggt
     7621 caatctgcag gaacagtatg gatcgctatt tcatctaaag acggcgtggt ttcggaaaaa
     7681 tatcactttg ccagagatcg tgctgctatc agatataatg cggttatgca aggatttgat
     7741 cttttgagaa aacagctttt aagaaaatga taaatgcgaa tatttgttcg gttttttctt
     7801 gctttttttc actaaaaaag atatgataag gataatgaaa tgttgctctg atttgtaaat
     7861 ataaggagga ttatcgtatt ggcagatgat cgtaaagcag ccttagatgc tgcactgaaa
     7921 aaaattgaaa agagctatgg taaaggctct attatgaaat tgggcgaaaa aatcgaccaa
     7981 caaatctcta caattccaag tggctctctt gcgttagatg tcgctttagg tgtcggcggt
     8041 tacccccgtg gacgtatcat cgaagtatat ggacctgaaa gttcaggtaa aacaacagtt
     8101 gcactacacg ctattgcaga agtacaaaaa aatggcggaa cggccgcttt cattgatgct
     8161 gagcatgcgt tagatccgca atatgcacaa aaattaggtg tgaatatcga tgaactactt
     8221 ctttcacagc ctgacacagg agaacaaggt ctagagatcg ctgatgcttt agtatcaagt
     8281 ggggctgtag atatcgtagt agtcgattca gttgctgctt tagttccacg agcagaaatc
     8341 gacggcgaaa tgggtgactc acatgtcggg ttacaagcac gtttgatgtc tcaagcattg
     8401 cgtaaactct ctggttcgat caacaaaaca aaaacaatcg ctattttcat caaccaaatc
     8461 cgtgaaaaag taggcgtgat gtttgggaat ccagaaatca ctcctggagg acgcgcattg
     8521 aaattctacg caacgatccg tttggaagta cgtcgtgcag aacaattgaa acaaggtaca
     8581 gatattgttg gtaaccgtac aaagatcaaa gtcgtaaaaa acaaagtagc acctcctttc
     8641 aaaatcgctg aagtagatgt catgtatggt ttagggatct cacaagaagg agaacttcta
     8701 gacatggcag tagaaaaaga cattgttgat aaaagcggtg cttggtattc ttataaagaa
     8761 gatcggattg gtcaaggacg ggaaaacgct aaaatctata tggctaatca tcctgaaatg
     8821 atggcagaag tttctgctct agttagagca gcttatggta ttggtgaaga agtcgctgtc
     8881 ccagaagatg aaaaaggaca agaagaattg cctttagtag aagaataaaa attatgataa
     8941 acaaaaacgg ctggtctgaa gagtaggtac tctccttgac ctgccgtttt tttattgaat
     9001 aaagaaatcg tgagttattt aattatacga gtgggtacat aaaataaaaa tatctgttca
     9061 tctacaaaga ataatttaat agaaaagttt ctcaatttaa aggtttacct atatgaaact
     9121 agtgtcattt attttaaaaa ccatcgttta atgaataagt agaaagccgt ttctttgttt
     9181 atttaaagtt gacactacgg ggtacaaaca ttagaataaa gttgtgcatg ttttttgtac
     9241 acatatatgc gaagatgcat attgacgagt ttagaataat ttatcataga agctgtatag
     9301 atgataaaaa tgaaaaagat agtatggagg tggattttat ggtattcaac attctcctcg
     9361 ctatcatcgg tttaattgtc ggtcttggtt tagggtttat ggtcgcaaag tcacgtcacg
     9421 agaaagaaat agctggtgca caatcatcag ctgctggaat catcgatagt gcaaaaaaag
     9481 aagcagaaac tctaaaaaaa gaagcattgt tagaagctaa ggaagaaaat cagaaatacc
     9541 gttctgaagt tgaaagtgag ctgagagaaa gtaagttaga attaaaatct caagagaatc
     9601 gtttgattca acgcgaacaa acgctgaatc gtaaagatga ctcgttagaa aaacgcgaga
     9661 actcacttga aagtaaagaa gaagcactta gttcaaaaca gcaattaatt gaagagcgag
     9721 aaaaagacgt tgaaaaaatg attgaagatc aacaaaaaga actagaaaaa attgctggtt
     9781 tatcaaaaga ggacgcacga gatgtcatca tgaaatcgac tgaagaagag ctgaatcatg
     9841 agttaactct aatggtaaaa gaatctgaac agcgtgccaa agaagaagca gatcgcaaag
     9901 cgaaaaactt attatcatta gcgattcaac gctgcgcagc agatcaagtt tcagaaacga
     9961 ctgtttcagt cgttaccctt ccgaatgatg aaatgaaagg tcgtatcatt ggacgagaag
    10021 gtcgtaatat tcgaacgctt gagacgctga ctggtatcga tttaatcatc gatgatacac
    10081 cggaagcagt tgttctttcc ggattcgatc caatccgaag agaaatcgca cggatgacct
    10141 tggaaaaact gatacaagat ggacgaatcc atccagcgcg tatcgaagaa atggtcgaga
    10201 aatctcgtaa agaaatggat gaacgtattc gcgaatacgg ggaagaggca gcgtttgaag
    10261 tcggtgctca tacacttcat ccagatctaa tcaagatcat cggacgtctg cgattcagaa
    10321 ctagttatgg acaaaacgtc ttgaagcatt cggtcgaggt tgcaaaatta gctggtatct
    10381 tagcagcaga gcttggcgaa gatatccaat tagcaaaacg tgccgggttg ctccatgata
    10441 ttgggaaagc acttgaccat gagattgaag gatctcacgt tgagatcggt gcggaattag
    10501 ctgccaagta caaagaaaac ccagtagtaa tcaacgcgat cgcttctcat catggagatg
    10561 tggaagctac ttctgttatc tctgtccttg tagctgcagc agatgcgctc tcagcagcaa
    10621 gacctggtgc gcgaagtgaa tcattagaaa actacatccg tcgtttaaga agtctggaaa
    10681 atatttcgaa cagcttcgcc ggtgtagaat caagttacgc agttcaagct ggacgcgaag
    10741 ttcgggtaat ggtcaagcca gaagaaatct cggatttaga cgctgttcgt ttagtacgtg
    10801 atattcgtaa acgaatcgaa gaagaactgg attatcctgg acacatcaaa gtaatcgtta
    10861 ttcgggaaac tcgtgcaact gattatgcta aataataaaa aatacaaaag aagagccgcg
    10921 gcaaaagcca cagctcttct ttttttgtaa ggtacgaaaa aacattgcta tcattatttt
    10981 ttattcaaca tctcaagatc tttctcgatc gtcgtattga ttcctttgat gattgctggg
    11041 atgaatcctg cttcttctaa ggcaactaca cctgcaacag ttgtaccgcc aggagaagaa
    11101 acttggtcta caagttccca tggattttta tcacttgaaa ggaatttttg acaagaaccc
    11161 ataattgctt ttgcagcaat ctcagtggct gtctgtttgg acaaaccatt cagtacacca
    11221 gtccgtccca tcgcatcgat cagcatgtaa acgaatgcgg gtgagctgcc agcaaggcca
    11281 atgaatgtac tgaaatctga ttctgctaaa ggatagacgc tgccgattgc ttcaaaaatc
    11341 atcgtagctt gctgaagctg ttcctccgtg gcagcttcat ttttgcaaat cgcagaaacg
    11401 cctgctccta cgctgacatt gacattcggc atcacacgta cgatggaggc ctgaggatct
    11461 tcaagcgctg tttctaaatc tgttaatgat tttcctgaag caatagaaat gaataagact
    11521 ttctggctat attctcttag ttcttccaat actttttcaa tgatttttgg tttcacggct
    11581 aaaagaacga tatctgattg tgatgcaact tcctgattac ttttgcatgc atggacgcca
    11641 gtttcagcag caaatttttg tgcattttca aaatgtccac tggaaacgaa cacctcttct
    11701 ttcgagactg ttcctttttt taataaacca tcaataatag cttgtgccat attccctgtg
    11761 ccgataaatc ctactttcat gttatcctct cctttagcat taggatatct ttctttttct
    11821 atcatcgcaa gcaaagaaca ggtctaaagt ggaaaaatta aaataaataa gataacattg
    11881 gggtatgaca caaaagtata caagttagga atgggaaaat gtgaaaaata aacgatactt
    11941 gatgctgcaa tggataaagg aacatagtct gattttaaaa ctgatttttt tcggctccgt
    12001 gttagtcttt gtggcaaatc aagtcgcaaa tattgctaat ggcatgagtt ggcaagatat
    12061 ttggcaaagg atgggacaac aaagccgcat gacgatcctg ctcatgattc tggctggact
    12121 gctgggtgtc actccgatgt tgttgtatga ttgggtgacg atcagagtct tagaaaaaca
    12181 aggaaaacca aaaatggaaa gaaaagagtt tcttcttgct gcgtgggtaa caaacacgat
    12241 caacaatctc gccggttttg gtggtgtggt aggagcaagt ctgcgggcta gtttttatgg
    12301 aaagcagaca aatagaaaaa tggttttagc cactgtatca aaagtcgcac tatttatgct
    12361 gactggtttg tctgtatggt cgtttcttac atttattgat gtctttttta ttcagtcaga
    12421 aagtattttt cgaagctatt ggatctggct tttcggcggt agttttttag caccagtcat
    12481 tttctttttt gcttatctaa agagaaagac attgttcttc gagttttatc caaaaggctt
    12541 gcttggtctg ctactggcat cctttggaca atggacggga gcccttgcgg tatttttgat
    12601 catcggcaag ttgatgagaa tagatatttc gatgctttcg atctatccta tgttcatgat
    12661 tgctacattg ataggaatgc tgacgatggt cccaggtgga atggggactt ttgacgtttt
    12721 gatgatctta ggaatgagtc aattgtctgt tcggcaagat gtagcagtag tttggctatt
    12781 atattatcgg ttgttttatt atgttctacc ttttatgacg ggacttctat tgttcatcac
    12841 acatacagga gtcaaaatca atcgattttt agataacatt ccgcgactgt ttttccaaaa
    12901 ggttgcgcat atcgtgctcg taagtgctgt ctattttgcg gggatcatga tggtacttct
    12961 atcaacaatc actaatctgt ctgcaatcag tcggctattt caagtattat tgccgttctc
    13021 attcaatttt ttagatcaga cagtgaatct attggttggc tttttattac tcggccttgc
    13081 tagagcactt tcagtaaaag tcaaaaaggc atttctgcct acgattgctt tactatcttt
    13141 tggtatttta aatacaatca cacgtacgtt atcttggcag ttgatattgg tatatgtctt
    13201 gattctgtta gctgtgtggt tatccagaaa agaattttat cgaacaaaat tcgtttattc
    13261 ttggggagct ataatctttg atgctagctt gttcggcttt ttgtttatcg tttacgcaat
    13321 tgcaggctat cacagcggac gctttttagt cggtcgaata acaggaaatc attttatttt
    13381 gtttccttca gatgatgtgt ggttctcagg gcttattggt ttgggcatct cattgctagc
    13441 tttagtggcg ctttatcaat atttagctga agatactacg aagcttggca gtgagtacga
    13501 aaaagaccgt tttgaccgtt tattggaaac atacggaggc acagctacaa gccatcagct
    13561 tcgcttgtca ggttacagtt atttttatta ccaagaagac gaccaagatc aagtcgtttt
    13621 tggttatcag atcaaaggaa acaaatgctt tgttttagga gatccagttg gcaatcctaa
    13681 aatgatgcgg gcagcaacga atcagtttat gaaacaagca gattgtttag gctatcagct
    13741 tgcgttttat aagatatcag aaagctacgt agtgatgctt catgaattgg gttttcactt
    13801 cacgaaagta ggtgaagcag ggattgttga tctagcaaca gaagacttgt ctcggctgag
    13861 tcagcaactt gaatatcaac gtttgatgaa agaaggatac cattttactt ggtacgatca
    13921 actgccggaa gaattacatc aagctgtaaa tgaagtttca caaaaatggc ttcaaggtgc
    13981 acgagaaaaa tacttctctg tgggacgatt tgataaatca tatgtattat ctagcggtgt
    14041 cggtattgtc cgtaaagatc aaaaagtcgt tggcttcatc accgctcagc cgatgacgaa
    14101 gaagcaggcg ggatatgatt tgttgcgggt cttacctaaa gaatctgagg aattagctga
    14161 ctatctgtta gccaatctgt ttattgtata cgagaaaatg ggctactcgg aagccaatct
    14221 aggcttagct ccattagcta atgttggaga aacagatttt tctttctttc aagaaaaagt
    14281 catgcatatc gtatataatt atggtaattt tttctattcc ttccagtctg cttatgaaaa
    14341 gaaattaaaa tatgtcgatc attgggaagg acgttatttt gcttatagga aaggaagtaa
    14401 tttcggattt gctaccatgc agctactcag tctgatagga aaagaaaaag gaaatgttcc
    14461 gagtttggca gaagaagtgc tcacagaatt ataagaatca ctagaacttg tttggattat
    14521 gaagagaaaa ttctgtgaaa gctgttgaaa cgatgacaaa atcaaccact aggcagacat
    14581 aagctatact tttatttttc cctatgatag aatacttgcg acttagactt ttcgaatggg
    14641 tgagaggatg gaattgattg aaaaagcgcc agccaagata aatttaggac tggatgtatt
    14701 atatagacgg caagatgggt atcatgagtt ggaaatggtg atgtcgagcg tagacttggc
    14761 agatcatcta ttttttgaag aaataacaga agacaaaatc attgtagaaa caaataaagc
    14821 atttttaccg gtagatgaac ggaacaatgt ttatcaagct gctttattgc taaaaaataa
    14881 atacaatatc aaaaccggtg tcaagatctt tgttaagaag aatattccag tagcagctgg
    14941 attaggcgga ggaagtacag actgtgcagc tgctctaaga gggatcaacc aattatggaa
    15001 tctgggctgt tcgttagaag aactggcaga aatcggtttg gaagtcggta cagatgttcc
    15061 ttactgtgtc tacggaagta cggcatttgt cggtggcaaa ggggaagaaa tccaagtatt
    15121 accttctatg ccacagtgct gggttgtgtt ggtcaaacca agaatgagtg tctctacacg
    15181 tacagtgttt agagaagtag atacgcaagc catccaacat caagacatcc acggacttgc
    15241 tgaagccatc cgagagcaag attatcagca gatgatcaaa aaaatgggaa acagtttaga
    15301 agatataacg ggaaagcggc atccagtcat ccatcaaatc aaagagcgta tgatcaagta
    15361 cggagcagat gctgcattga tgagtggaag cggaccaacc gtttttgcct tgtgtcaaca
    15421 ttattcaaga gcgcagagag tatataatgg gttaaaaggt ttttgtgagg aagtttattt
    15481 agtacgaacc ctgcaataaa atataagaag taaaataggt gatgaaaaac tgaggaatcc
    15541 taaaatggta ttcttcagtt ttttattgta gtgaaatggc tagatatgag cagcaactca
    15601 tccattttac gatttaatca agaatataca tgcttaaatc agaaattgat ggatttctat
    15661 tgactgtgtt tttttatttt gatacattag ttcacgttaa taaatcgtaa aattccgatt
    15721 tgttcattga aaggagcata taaatgaaaa aatatctttt gacagctacg gtaatgatag
    15781 gtgctttact gtttgcagca tgtggaaata cgaacaagga agcagacaaa aaagaggatt
    15841 tgacgatcgt tacgactttt tatccgatgt atgattttac aaaagagatc gtaggggatg
    15901 aaggaaacgt gaaacttttg atccctgctg gaacggagcc gcatgatttc gaaccaagtg
    15961 caaaagaaag agcagaaatt tccgatgcag atgttttcgt gtataatagt tcagacatgg
    16021 aattttttgt ggattcgtta aaggattcag tggatagcaa acagaccttg atgatcgaag
    16081 cagctaaagg aatcgaccgt ttagaatcgc aagaagcaga cgaacatgaa gaatcagaag
    16141 aagggcatgg tcattcacat gaatacgatc cacatgtttg gctcgatccg gtattagcta
    16201 taaaagaagt cagaacgatt gctgaagaat taggcgaaaa atatccagat aaaaaggaaa
    16261 cttttacgaa gaatgccgat gcttatatca aaaagcttga agcactggat caaaaatact
    16321 ccgatgagct aaaagatgca acaaaccgca catttgtcac tcagcatgct gcttttgctt
    16381 atttagcgaa tcaatacgac ttaaaacaag tagcgatttc gggcgtttca ccagatcagg
    16441 aacccacacc aagtcgctta gctgagctaa aagaatttgt gaagaaaaac aacatcaaag
    16501 tgatttattt tgaagaaaat gcctcttcga aggtagctga gacattgtcg aatgaaacag
    16561 gagtgaagct agaagtgttg aatcctttag aaagcttgac taatgaacaa atcaaagcag
    16621 gcgaaaatta tatttctgtc atggaaaaaa atctgaaagc tttaaaagaa agtatcaatt
    16681 aagttttaga atggaggttg taaggtgcgt tatatcgaaa tatctgattt gaccttttac
    16741 tatgatgatg aacctgtact agaagatgtc tcttatcatg tagatgcggg tgaatttgtc
    16801 attttaactg gagaaaatgg agcagccaag tcaaccttgg tgaaagcatc gttaggatta
    16861 ctgaaacctt caaaaggaat gataaagatt gctgagaaaa atcaagaagg aaaaaaatta
    16921 agtatcggtt atattcctca acaagttgca tctttcaatg caggttttcc tagtacggtg
    16981 atcgaattag tccgttcagg aagatatcca agaggccgct ggttcaagcg tctctctgaa
    17041 aaagatcatc aacatgtaca gcgtgcttta gaagctgtag ggatgtggga tatgcgaaat
    17101 cggcgaatag gtgaattgtc tggcggacaa aagcagcgaa tcagtttagc gcgtattttt
    17161 gctactgacc ctgacttatt tgttcttgat gaaccaacaa caggtatgga tgaagaatcc
    17221 agaaatgaat tttatcgtct tctccgacat agtgcgcatg tccatgggaa atcagttcta
    17281 atgatcaccc atgatcatga agatatcaaa cagtatgcgg acagacagat ccgactggtg
    17341 agaaaggagg attctcaatg gcgttgcttt catatgaatt catgagaaga gcgtttcttg
    17401 ccgctatctt cattgccgga atcgccccga tgctaggtgt gtttttagtg atcagaagac
    17461 aatcgctgat ggctgatacg ctttctcatg tctctttagc aggggtagcg ttaggattct
    17521 ttttgaacct taatcctaca cttacgacca tgctagtcgt tgtattagca gctgtgatat
    17581 tggagtatct gcgaacgatc taccgaacct attcagaaat ctccattgcc attttaatgg
    17641 ctggtggttt ggcgctagcg ttagtattga tgaatttgag cggagggaat tcagcaacaa
    17701 gtatccaatc ctatctgttt ggttcgatcg tgaccattac atgggagcaa gtcatctttt
    17761 taggaatctt attcgttgcc atcggtatat tgttcttttt attcaaacgg ccgatgtatg
    17821 ttctgacttt tgatgaagat accgcccacg tggatgggct acccgttcat ttgatgtcga
    17881 tgatgttcaa tgtgattact ggtgtggcta ttgctgtgat gatcccgatt gccggtgcac
    17941 ttttgatatc cgcaatcatg gttcttcccg cagcaatcgg tatgcgatta ggcaaaagct
    18001 tcaatgtcgt gattgctatc agtgtttgtg taggatttct agggatgctg tcaggactaa
    18061 ctagctcgtt ttatctagat acacctccag gagcaacaat cacattagtc tttattgctt
    18121 tgttcctgat tgtaaatatc atcaaacgaa tattggttgc cttcagaaga aaaaataact
    18181 aaattataaa gaaaagaagc cttataaaaa cgaaaaattt tttataggct tctttttatt
    18241 ttaataaata ccgaactatt ttcttatttt ttcataaaat gctcgaattt ttgaagaaaa
    18301 acttatataa tgtaaaacga taaaatttct taaaaggagt tggaataggt gaaagtacgt
    18361 cgcagtgaac gtctagttga catgacacag tatttattga atcacccgca tgaattgatt
    18421 tccttaacaa gctttgccga acgttatgaa tctgctaaat cttctatcag tgaagatttg
    18481 ggaatcatca agaaaacatt taaagaacga gggtatggaa ccttggaaac aatcccgggt
    18541 gctgcaggtg gtgtattgtt catccctgaa atttcatatg aagaggcgaa aaaatatatt
    18601 gaatctctag cagaacgtct atcagaacaa gaccgtctgc ttccaggagg atatgtttat
    18661 ctctctgatt tgttaggaga acctgaactg ctgcgtcaag ttggaagaat cattgcttct
    18721 aaataccttg gtgaaaaaat cgatgctgta atgactgtag ccacaaaagg cgtacctatt
    18781 gcccaagctg tgtcctatta tctgaatgcg ccattcgtta ttgtacgccg ggactcaaaa
    18841 ataacagaag gatcaacagt aagtgtcaac tatgtgtctg gatcttctga aagaattgaa
    18901 aagatggaac tgtctaagag aagtttgaaa cgtggttcac gtgtccttgt agtggatgat
    18961 ttcatgaaag gcggcggaac agtcaacgga atgaagagct tgattgaaga attcgaatca
    19021 gaacttgttg gcatcactgt atttgccgaa tcaaaattca acggtcatcg cgccatcgat
    19081 gattatacgt cactgcttta tgtcaaagat gtagatactc aaacgaaaaa tattacggtt
    19141 gtgccaggaa attatttttc agaataagag gttgtgaaaa agccgcttag tttcgagtag
    19201 taagaaaaat tttgtgaaaa tggctttcca cattttttca taaatttggc ttaataccga
    19261 gaaactggct tttgaacacc gtttatctag aggttgtgaa atcagcgtcc tgtcctgagt
    19321 attagctcaa taaacggaaa aattgctcct catattttgc cgtttattga gctaataccg
    19381 aaggtcagct gattgaaaaa cgtttaatga agaggttgta agcctaccgt ttaactctaa
    19441 gtcacaagaa acgatttcaa aaaacagctt ttcatgtttt atgagtagtg tgacttgtga
    19501 ccgaggagtt ggcaggagag caccggttac caaatagata ggcccgttat ggtctatcta
    19561 tttttttatc cagaaaacgc tgggataaaa gaaaatcact tgttttgttg aaaaaatggg
    19621 tatactggga taaactgaaa tcagcaaact atatttagga gtgacgcagt tggacgcacg
    19681 ttatgcaatt attttggcgg cagggaaagg tacccgcatg aagtcaaaat tatataaagt
    19741 tttacatccc gtttctggta aacctatggt agaacatatc attaatcggg tcagtgaaac
    19801 gaaaccagat gaagtgatta cgatcgttgg acacggagca gaacaagtta aagcacaact
    19861 aggagaacgc agtaaatatg cgttacaagc agaacaattg ggaacaggac atgctgtttt
    19921 gcaagctgct tctttcttgg aaggaaaaaa aggaacaacg ttagttatca gtggcgatac
    19981 accattattg actactgaaa cgctgaacaa tctttttgaa tatcaccaag gaaaaaatgc
    20041 aagtgcaacg atcttgacgg cacaagcaga aaacccaaca ggatatggac gaatcatccg
    20101 tgaccatatt gggatcgtag aaaaaatcgt ggaacaaaaa gatgcaacac cagaagaagc
    20161 tcttgtacaa gaaatcaata caggaactta ctgttttgac aacgaagctt tgttcgatgc
    20221 gttaagcaaa gttggaacaa acaatgctca aggtgaatac tatctaacag atatcatcga
    20281 aatcttaaaa gaagaaggcc atacagttgc tgcttatcaa acggatgatt ttgaagaatc
    20341 aatgggagtc aatgatcgga tcgcgttagc taaagcaaat gagatcatga gaaagcgaat
    20401 taaccagatg catatggtca atggcgtaag ctttgtcgat tcagcaacga catacatcga
    20461 tgcaggcgta gaaatcggtc cagacacatt gatcgaagca ggtgtacaaa tccaaggaaa
    20521 tacagtcatt ggttctgatt gtgtcatcgg gtcacattca aaaatcgtag acagtaggat
    20581 tgaggatcac gtcgtcattg aaaactcagt cattgaaagc agccatgtca agaaacatgc
    20641 agatgtggga ccttatgcac atttacgacc aaaagctgaa attggcgaaa acgtccatat
    20701 cggaaacttt gtggaagtga aaaatgctca aatcggcaaa ggaactaaag tcggtcactt
    20761 gacttatgtt ggtgatgcta cgctgggaga agaaatcaat gtcggatgtg gtgttgtctt
    20821 cgtcaactat gatggaaaga acaaacatca tacgactgtc ggcgaccaca gctttattgg
    20881 ttctagcacg aatatcatcg gacctgtcga ggtagctaaa aacagttcga ttgctgccgg
    20941 gtcaacaatt acagacaata ttccagaata tgcgctggca attgcgagag caagacaagt
    21001 caataaagaa ggatatgcaa aaaaactccc ttatctaaac taagactttt gcaaaatcac
    21061 ttgatttatt taaaaaatat gactactatt tattaggtag aattctagag ttgtgaaaaa
    21121 gaaagtggag gtccccatgt caaaacatta ctttgaccca agattaaaaa tttttgcgtt
    21181 aaattccaat cgcccattag cagagaaaat tgcagcagca gtcggcgtcg agttaggaaa
    21241 atcatctgtt actcaattta gtgatggaga aattcaagta aacattgaag aaagtattcg
    21301 tggatcgcat gtctatgtta ttcaatcaac gagcagccca gtcaatgata acttgatgga
    21361 gttgctgatc atgattgacg cactgaaacg agcaagtgca aaaacaatca acgtagtcat
    21421 gccttattat ggctatgcgc gtcaagaccg taaagcacgt gcacgggaac caatcacagc
    21481 gaaattagta gcaaacatga ttcaaaaagc tggtgcaaca agaatgctga ctttggacct
    21541 tcatgcagtc caaatccaag gtttctttga tattccagtc gatcatttga tgggtgcgcc
    21601 acttattgcc aattatttct tagaaaaagg aatcaaaggc gatgatgttg tcgttgtttc
    21661 accagaccat ggtggtgtca cacgggcacg taaattagcg gaatttttga aatctccgat
    21721 tgcgatcata gacaaacgcc gtccaaaagc aaatgttgca gaagtaatga atatcattgg
    21781 acaagtcaaa ggcaaaactt gtgtactgat cgatgatatg atcgatacag caggtacgat
    21841 cacattggct gccaatgctt tgaaagaagc tggtgcaact agtgtgtatg cttcatgtac
    21901 acatccagta ttatccggcc ctgctttaca aagaatagaa gattcagcta tcgagcgtct
    21961 agttgttaca gactcgatct atcttccaga agatcgtaaa atcgacaaaa tcgatgaagt
    22021 aagtgttggc ggactaatgg gagatgcaat caaacgtatc catgaaaaca aaccagttag
    22081 tccattgttt gaaacaaaaa ataaataata aataaactca caaaggggag tccaattggg
    22141 ctgagattga atctatttct aaacccttcg tacctgtatc ggttatgcga gcgtaggaat
    22201 tgtgaataaa cagctgtttg actattttcg aaagctgctt cctcctttgt gactttttta
    22261 ttcaaaggag gaagtttttt tatgggaaaa aatagagttt gggtagaagg aacgattgtt
    22321 gcagcgttag cgatggtgtt gtcgttgatt ccgatccaga tcggcagcag tttttctatt
    22381 tcattagggc agattccatt gactcttttt gcgttaagaa gagggacaaa ggcagggatg
    22441 ctggcaggtt tcatttgggg catcctacat tttccgttgg gacaagttta ttatttaagt
    22501 gtcgttcaag tgttgattga gtacccgatt gcctatactt ttgcaggcgc agcagggctc
    22561 atggcttcaa atgtccaaaa aggctatgta gacgataata cgaaacaaat tagaaaaagc
    22621 atcgtgtttg gtgcattgat tggcgcactt tccagatatt tttggcattt tattgcagga
    22681 gtggttttct ggggagcgta tgcactatgg gggatgaatt cttggctctt ttctttcgta
    22741 atgaatggtg ccagcgggtt gagtacagct attgtaacga tcatcgtttt attagggatc
    22801 caacaaacga ctccttcgat ttttgtgcca gataaacgac aggagctgta aataaaaaag
    22861 aagcaagaaa cttgagacag aatcatgtcc tagtttcttg tttccttttt attttttgat
    22921 aagaaataga tctacttcaa tggcagataa gaaagatcgg catcttctac caattgccag
    22981 tgtcgatcgg tgacagagta gattaaagta gcgatgattt cctcttctcg ataccgggtg
    23041 ccttttaatt tccaaatata atcttcaatc acatagattg ggatacgata tttaccatcg
    23101 atcaaaccaa cttgatcaaa agaaaaaatg atcccttcct gcatcaaatt acgcatttct
    23161 ttcaataatt ccacactgat tttttcaaca ggtgtttctt ttttgaaaaa agattttttt
    23221 acattgatct tatctgacaa gatcgaactt aatttactag caaaaatcct ataaaattca
    23281 tctaaatagc gataataaat ccgattcagc tgatcattaa gatcgaagta tacaaaatta
    23341 ttctgtagtt tataaaaaaa cggcgaatga agatgtgttt tcatatgacc aaaatataat
    23401 aattctgaaa tttctaaagg tgtcaattct ttcagcatcg ttaaatcagt aaagtcgatc
    23461 cacttcaatt catcgaatcc acgcctgtta cgagaagagg aaaagaaacg ctgaatattt
    23521 tctgtaccac ggattacttt cagccctgta tgggattcat attctccagc agaagcagat
    23581 ggatcaagca agagcaagtt ttgaggaaaa tgcacgatac tgtttgcaaa gtcagtagca
    23641 gtcagccctt ttgttaaaac agcattactt gtggtatcaa tatgtacata aactgactca
    23701 gtcatcatgt tcctcctctt atttcctctt aatctctttt attttacaac atttgcatta
    23761 aaaaagcttg tttcttttat tttaaaattc cattacaaaa tcacgttttc attgaaaaca
    23821 ctctcatttt caattaattt ttgtgatttt tgtatttttt atttaaagga tgttttatga
    23881 attgttttct tatggaatct acaaagtcga atacgagact catgaccttt ttatgacgta
    23941 ctggtatact tgaagaaaaa ggagagcgcg atggagataa aaaaagttta cttttcacag
    24001 aaaatgtcgc tgtattcgcc tatccagcaa gcaaaacaac cccttccatt ttttcaaagt
    24061 catcaagaaa gtagagacgc tttttggcgt tctgcaaaaa agcaatgtgc ttttaaagca
    24121 gtatcgaaaa aatataccgt ctattttact attgctaaaa gtagaggaga tgttattttt
    24181 aataatctgt tgattgaagg tgaaactttt gaatggaaaa aattttttcg gtatatggaa
    24241 gcttgcgctc gtttttttat taaagaaaac tgttgttttt tatttcaatc tcctctatca
    24301 gaagaatggt tgcgtatttt tcataaaaac ggctatcaag gaaccacaca attaaataaa
    24361 aagctggtct atcatacagc acttgtttta ggtggaggag gagcacacgg tgcttatcag
    24421 attggtgtgt ggcaggcgct gaaggaacat gatatcaact ttgagattat cacaggtacc
    24481 tcggtaggtg cgttgaacgg cgcattgatt ttacaaggag atatgaaaaa agctgtgaat
    24541 ttatggaaaa agctgtcgac aagacaagtg cttgcgttac cagaaatggc tgcagttgaa
    24601 gaccttcgtg aacgcttcta tcgagaaaga cgccaaatga cgaaaacagc actaattgaa
    24661 ggcggggttt cttctgctcc attggaaaag atggtcaaag acaatttgga tttgtcctta
    24721 ctcaaacata atcccaaaat acgtttatat acggtgtcta ctaaactgcc agaactagcg
    24781 gaagtagtga ctgatatcca acagacaaaa acagaagaaa taccagattg gatactcgcc
    24841 tctgcttcat tttatcccgc tatggcttat cgaaagatcg gaaaaaataa atatgcagac
    24901 ggtgggtatc gcaacaaaat acctattgat attgcgatta acaaaggagc aacagaggca
    24961 tttgtcgtag atgtgcaagg gccaggccca gcgaaaaaaa ttcgtatgcc agacattttt
    25021 atccattgga aatgtcaaac attgtggaca ctaggcagct ttctgctttt tgatagccaa
    25081 aggaaccaat taaatttaca attaggctat ctggaaatga aaaaacgatt aggctattac
    25141 tatgggaact ggtatacatt tcattctgtt aaacctgcaa gaacatactg gcgaggattt
    25201 ttgtcttact taacaaaaga aattcagtta gaattatctt ttttcaaaag cataaaattt
    25261 tggcaaaaac tgcgtaattt atataagaac agggtcgtac cagagacatg cggattggcg
    25321 atgctagaac ttttagcaaa aaaacatttt ttactgccca atgaaatcta tcaagtagac
    25381 atgatgattc aaatgatctg ccaacaagat ataaaaagta tgccggatga tttattggca
    25441 caaattggtc aattaagtac tgaagaatgg cgaaggtatc gaaaatacca gcaaaaaata
    25501 caaaatgagc gcacgaaaac tgtttatttt gattatctgc tacaaggaaa acgcaaggat
    25561 cgtttgcagc atgaattgct taagcaacca gttgacacat tactgatcct atatttatat
    25621 tatctcaagg aggaacaacc atggcacaag aattttcata tgaaatcgta gaagaaatcg
    25681 ctatattgtc agaaaacaac aaggggtggc gtaaagaact gaacctagtc agctggaatg
    25741 gtcgcccgcc aaaattcgat cttcgagatt gggcgccaga ccatgaaaaa atggggaaag
    25801 gcctgacatt gacgaacgaa gagtttgaac aactgcaaaa agcaatagaa aatatgtaat
    25861 gaggaaggac cctgtcgaat gaaaagtatg actggttttg gcaaagccat ccgcgaaaca
    25921 aaagcgtatc agttagatat tgaaataaaa agtgtcaacc agcgttttct agatgttcaa
    25981 atacgaagtc ctaaaatcct gaactttgtt gaaaacgatt ttcgtcagct tattaaacag
    26041 cagttaagcc gcggtcgaat cgaagtattt gtgaatctag cttatattgg caaaaacgaa
    26101 aaacagatcg tcatcaactg ggaattgatt gattcactta tcactcaatt gacagacgga
    26161 attcaacaac gatacggtag tcaaaatcaa gtgtcaatta gtcggatggc ggaacgcttc
    26221 gcgctggacg aaaacttcgt aacggtagaa gaaaaaccgg aagagagcat ggaagaattg
    26281 gcacagttag ctctagatac attgaaaagc gcacttgatt ctattgaggc gagtcgagaa
    26341 aaagaaggtc aagctttagc tttagtgttg aaaaagaata ctgaagaatt caaagaagta
    26401 ctaaaaaaac tcgatagttt tgtagaaatc tatgaaaagg aatttcaaat aaggtatcaa
    26461 aaaaagctgg aggaatttct tggagcgaaa gtcgatgagc aacggttgtt gacggagcta
    26521 gccatcttgt tggaacgcgg agatattcac gaggaactgg atcgtctggc gatccatgta
    26581 caaaaactag atgaacttat cgaagcacaa tgtcctgtag gtcgggaatt agatttcctg
    26641 atccaagaaa tgaatcggga aatcaacacg atcggttcca aatcaaatgc aatcgaaatt
    26701 aaaaatcaag tcgtccagtc taagacgatc ttagaaaaaa tcagagaaca agtacaaaat
    26761 attgaatgac gaaagaggta gagataatga aatattatga cgtaacgttc cacgaattaa
    26821 gtggaaaaac tgtggttaaa cgggatatcc cttctgaaaa aaacggcttt gacgtatgga
    26881 aagatgcctg tgcagactat aatgagaacg aactgtttat tttgatcaac gacggtgctt
    26941 acgtgacgat gaatcggaaa tttattgttc gaattgacac agaagaggta gaagatccga
    27001 cagaaaaagc acgttctcga aaagatgaga tcatgggtgt agtgaatact ttgtccaata
    27061 tgggctttta gcaaggatgc tgaacaaaga aatacaataa ataatcagaa ttggatttct
    27121 tcgcttagct tctttgctct aggaatacat ttaaaggttg tgacaaaatt cgtgtcacaa
    27181 tcttttttta ttttgaataa gtgatgttca aaaaccattt tctcggtatt aagccaaatt
    27241 tatgcaaaaa tgtgagaaac tattttcaca aaatttttct tcatactcga aactaatcgg
    27301 cttcttcacg acctcttata cacggcgttc aaaagcttgc atctgcgata ataaccccaa
    27361 ccaaacgaaa aacatgagaa gctgtttatg tttggctgtt cttattactt gatgcaggac
    27421 agctttttta cgacctcttg gtacagggta gcttttttac caatttctag ctataattaa
    27481 agataacctt gaaagttgca gaaaaaaagt gcaaaataaa cgataagtac aaaaagacta
    27541 gttgaaagga cgtcgtcatg tcagagcgtg gattattaat cgtgttgtct ggtccatccg
    27601 gtgtaggaaa aggcacagta cgaaaagcaa tttttgagag tgaggataat gattttcagt
    27661 attcgatctc aatgactacc cgtaaaatgc gtgaagggga ggtcgaaggc gttgattatt
    27721 tcttccgaag caaagaagag tttgaatcaa tgatcgaagc tggagagatg ctggaatacg
    27781 cagaatatgt cgggaattat tatggaactc ctttgtcata tgtcaacaag acattggatg
    27841 aaggaaaaga tgttttcttg gagattgaag tccaaggagc aaaacaagtc aaagaaaaag
    27901 tgcccgatgg tgtattcatc tttttgactc cgccagattt agcggaatta aaatcacgga
    27961 tcgtaggccg aggaacagat gcagatgaag taatcgaaga acgaatgaaa gttgcaaaag
    28021 aagaaatcga aatgatggct ctttacgact atgctgttgt aaatgatgaa gtgccgctag
    28081 cagttgaacg aatcaaaaac attattgcca gtgaacattt tcgagtagat cgcgtaatcg
    28141 gtaaatatat taaaatgtta aaggagatgt agtcttatga tgttaaaacc ttcaattgac
    28201 tcactattag atcgtgtaaa ttcaaaatat tctctagtga ttttagcaag taaacgcgcg
    28261 catgaattgg atgcaagtgc acagcctact ctagattctt ttgattcagt aaaaagcgtc
    28321 ggtcaagctt tagaagaaat cgatgcagga aatgtagtca atgatccaca tcctgaatta
    28381 aaacgtgaac gtttgaagat ggaagaagag gaacgtcaag cacaaaaaga gcgtgaacaa
    28441 cacgaattgg aatcacgtat ccgtgaagag caaaaaatgt aataaagaaa aatagagcga
    28501 acatctaaaa agaaaagcaa acccagctgt ttccttttca aacattcgcc acttattgtc
    28561 tggtagaaaa ggctgtgtct gaactttttg acacagttct tttttttctt atctgctgtt
    28621 aattttttgt taggatttgg cagaagataa gacaaatcca tcgatctcta gtaaaatagg
    28681 caaggaactt tttgtcgctt gataagaaaa gacaaaaaaa ggaggaaacg aaccatgaca
    28741 ttaacagctg aagtcattgt ggacgtacct actatgcaga ccgatcagcc attcacttat
    28801 ttggttccct ctgaaatgga aacagctttg caagtcggga tgcgagtaga agtccctttt
    28861 ggtaatggca atcgtcatgt ccaaggattt gttatggcta tccaaaaatc agaagaatca
    28921 gctaatccat ctttgaaggc agttattcgt ttgctagacc ttgctccggt ggtcaatgaa
    28981 gaattacttt cattagctga ttatatgaaa aaaattactt acgcattcaa aatcacttgt
    29041 ctacaaacga tgcttccgag tgtgatgaaa gcagaatatg ataaattgat ctatcctttg
    29101 gcagacacac cagaagttca ggtattgttt gatcagcgag aagtgatttc ctggaaggaa
    29161 gcagaagaag aaggcagcct ttctcaattg attcgctggc gccaagagca attagtagat
    29221 atcaaatatg aagtccatac acgaaataaa gtaaaaacga ttcgtttggt ccgttctttg
    29281 ttaactgaaa agcaaatcga agaagaatgg gcaaaattac ggcaaaatgc caagaaacaa
    29341 aaagaacttc ttttgtgttt atctgaactg agccaagaag aaccaattgc ttatttcaaa
    29401 aataaaggaa tcagtactgc tgtcttgaat caaggaaaag aaaagggctg gctggagttt
    29461 atagaatcag aacgttatcg cgacccttat aaagaccgag tatttgacca aacgacggca
    29521 ttggaactga atgcagaaca aaaaaatgct gtcgaacaaa tcatcacagc aggtcagcag
    29581 caaaaagatc aggtcttttt gctggaggga atcactggca gcgggaaaac agaagtatat
    29641 cttcaggcaa tcgctgatgt tttgtcagaa aataaaacgg ctattatgtt agtgcctgaa
    29701 atcgcattga ctccgcaaat ggtcgagcga ttcaaaggac gcttcggaga atcagttgcg
    29761 gtcttacata gcggactttc ccaaggtgaa aaatatgatg aatggcgcaa gattgagcgg
    29821 gaagaagcgc aagtagtagt aggcgcacga tctgctattt ttgccccgct gaaaaatatc
    29881 gggctgatca ttatcgatga agaacatgaa tcaagttaca aacaagatga gacaccgcgt
    29941 tatcatgcga gagatctagc aatttggcgc agtaaatatc atcattgccc aatagttcta
    30001 ggaagcgcga cgccctcttt agaatcaaga gcacgagctc aaaaaggcgt ttatcagtta
    30061 ttgcagttaa accaccgagc caaagcagct gcacagcttc cagctattga gatcgtagat
    30121 atgcgtgaag agtttcagaa tcatcggacc tctacttttt ctgctaattt gcaggaaaaa
    30181 atacaaaatc gattagacaa aaaagaacaa actgtcctac tgctcaatcg tcgaggatat
    30241 tcgtcttttg tgatgtgtag ggactgtgga tttgttcttc catgtcctaa ttgcgatatc
    30301 tctcttacac tgcatatgga tacacgttca atgcgctgcc attattgcgg acatgaagaa
    30361 cctattccca atcgctgtcc taattgtggc ggcaataaaa tccgctacta tggaacaggt
    30421 actcaaaaag tagaagagga actccgagaa ctttatccgc aagctaggat tttgcgtatg
    30481 gatgtagata ccactcgaag aaaaggggca cacgaacaga ttttacaaaa attcggtgca
    30541 aaagaagcag atatattatt agggacacaa atgattgcta aaggtttgga ctttccagaa
    30601 gtgacattag ttggggtgtt gaatgctgat acctccctga atttaccgga ttttcgttct
    30661 agtgagcata cattccaatt gcttacccaa gtttcaggac gagcagggcg agcggaaaaa
    30721 gcaggcgaag tcgtgatcca aacattcaat cctcaacatt acgccataga attggctaaa
    30781 aaacaaaatt atgagcagtt ttatcagcag gaaatgcatg tacggcatcg tggaggctat
    30841 cctccttatt actttactgt gaaaataacc gccagccatc ctgaagaaca agtagcggca
    30901 aagaaaatct ttcagattgc caatcagcta aaagaagtgt taagccctca aagcttgcta
    30961 ttagggccaa cgcccagcat gattttgaga gtgaaaaacc gatattatta tcaactaatc
    31021 atcaaatata aacatgaacc gaatcttcct catgttttgg atgaaatttt gaatggaagc
    31081 cagaaagaac agcgacaagg tctttttgtg gcaattgaca acgaaccatt aaactttatt
    31141 taagaaaggg ccaaattatg cgctatccta tattgatcca ccctaatgat aaattaaaaa
    31201 gaactgctca gccaatcgat gtaatcacgg acgagacaat cgctttatta gacaatttat
    31261 atgagacaat gattgcgaat gacggaatag ggatcgctgc tcctcaagta ggacaaaaca
    31321 agagaattgc tgtgatcgaa gtagatgaag gcgagaaatt tgaactgatt aacccagaaa
    31381 tcatcgaagc aaaaggtgaa agccttgacg tagaaggttg tctcagtatt ccgcatgtct
    31441 atggaacggt caagcgagca gatgaagtga ccgtgcgtta ttatgatcga gacggagaag
    31501 agatagaagt gacggctttt ggctatttag cccgtgcctt ccagcatgaa atcgatcact
    31561 tagacggtat cttgttcatt gagaaaatga tacagcagat cccggaagaa gaacttgaag
    31621 aatatatgga ggaacatctt gatgactaag ctagtattta tgggcacacc tgctttttct
    31681 gtgccgattc ttgaagggct gatagaatca ggttacgaga tccaggcagt ggtaacacag
    31741 cccgaccgtc cagttgggcg gaaacgagta attacgccga caccggtaaa agaagcggct
    31801 ctgaagcacg gtatccgtgt gctgcagcca gagaaaatct ccggttcgcc agaaatggaa
    31861 gaaatcattg aattggtgcc tgatgtgatc gtcactgcgg catttggcca attcttgcct
    31921 gaaaagttat tgcaagtacc aaaattaggg gcaatcaatg tccatgcttc tttacttcct
    31981 aaatatcggg gaggtgcgcc tgttcattat gccatcatga atggggagaa agaaactggt
    32041 gtaacgatca tggaaatgat caaaaaaatg gatgcaggcg gaatctatgc gcaagaaagc
    32101 ataccaatca ctaaacaaga tgatgtaggg acgatgtttg aaaagcttag ccttttagga
    32161 agaaagctat tgctagaaac attgcccaat attttggatg gacaaaaacc agtacctcaa
    32221 gacgagtcag aagcaacttt ttcgccaaac atcacacggg aacaagaagc aatcgattgg
    32281 cataaaactg cagaagaaat tgacaaccaa gtacgcggaa tgcgtccttg gccaattgca
    32341 ttcacgacct atgagggcat acgctggaaa ttattatcgg ttgaagtatt ggcagaaact
    32401 acagacaaag cacctggtac gatcatccgg aaagacaaaa aaaatctatg gattgcttgc
    32461 ggtaaacaga ccgtcttagc catcaatgaa ttacagccag ctggtaaagg aaaacaggcc
    32521 gtacatgatt tccttaacgg aagcggtcag caagttcaga ttggtcagca ggtgaaataa
    32581 tgaaaaaaat ccctaaaaaa atgaaaaaat cagtacgata tatggcatta ctgacattag
    32641 agcgagtgaa caagggtggc gcctactcga atcttttgtt gaatgagatg atcaaacaaa
    32701 gcgagctaaa tcaaaaagat gtgggtttat ttacagaatt gatctatggt acgattagcc
    32761 atcagcgact attggaattt tacgttgcac cattgatcgc taaagcaaaa aaagtggatg
    32821 actgggtcaa gacgcttctt tatctttctg tataccaatt agaattttta gataaagtcc
    32881 ctgcacatgc tattttaaac gaggcggtcg aaatcgcaaa agtaaaagga aatcctggga
    32941 cagggaaatt tgtaaatggt gtcttgagga attatcagcg tcaaggagca ccagatctgc
    33001 aaggcattgc tgatcctatc gaaagacttt ctgtcaaaat cagcctgcct ctatggatga
    33061 ctaaacgact ggtggatcaa atcggatacg aagaaactga aaagctcggt ttatcgctat
    33121 atcagcctag ccatgctagt gcccgagtag atacacgccg actttccaga gatcaggcca
    33181 tcactgtttt gcaagaagaa gagatcacag ctgaaaaaag ccaagtcagt ccatacggta
    33241 tagtagcgaa aaaagggcat ttagcttcta gctcattgtt ccacgatggc gtcatgacga
    33301 tccaagatga aagttcgatg cttgtagcgc cgtctatgca aatcgagcct gaacatcatg
    33361 tcttagatgc ctgtgcagca cccggaggta agacgacgca tattgcttct tttttagatc
    33421 cgtcatgcgg tggagaagtg gtcgctttag atgtacacga acacaaagtc aaactcattg
    33481 aagataatgc agcacgtcta ggtgtctctt ctgctgtcta cgctcaaaag atggatgcta
    33541 gagaagtcag taaacagttc gaagcagagc agtttgatcg gattttagta gacgcgcctt
    33601 gttcaggtct tgggctgctt cggcgaaaac cagatattcg ttataaaaaa cagccggatg
    33661 aattagcgaa tttaccaaaa atccaattgg aaattttaga aagctgcgca ccaactttaa
    33721 agtcttccgg tattctgact tatagcactt gtacgatctt aaaagaagaa aaccaagacg
    33781 ttgtttctgc tttcttggaa agacaccctg attttgagag gattgatgtt ttgaccaatc
    33841 ctatgctaca atcatcaatc gaagagaaaa tgttgacttt gtatccgcat cagttttaca
    33901 cagatggatt tttcatttgt tgtttgcgca aaaagtaatg aggtgaaaaa atgcagattg
    33961 aatatcaatc agatgtagga agacgacgta acacaaatca agactatgcc agtgtattta
    34021 ccagccgttc aggtctcaag cttgctgtat tagcagacgg gatgggtggt catcgtgccg
    34081 gagacattgc cagtcagatg gcggtaacga atctaggcga agcttgggaa aaacaagaag
    34141 ctgttgacga tgaaaaaatc gcccaatggt tcattcaatc gattcaagaa gaaaatatgc
    34201 tgatttatca gcgtggacaa gaacagccgg aatataacgg tatgggaaca acaatcgtag
    34261 cggccgctct gtcagatgac tggttcacga ttgccaatgt tggggatagc agagcttatt
    34321 tgattcgtga agataaaatc atgcaattga cggaggatca ttctttagtc aatgaactag
    34381 tcaaatcagg agaaatcagc gaagaaatgg cagtgaacca tccaaggaaa aatattttaa
    34441 cacgttctgt cggcatgcct ggaacagtcg aagtagatgt ttctactcat ctttggcaac
    34501 taaaagatcg actgctgtta tgttcagatg gcttgacgaa catgctgtct gaagaagaaa
    34561 taggaacgat catcaatcaa gaaggcccat tgtctcataa agtaactgag ctgattgatc
    34621 gtgccaatga agcaggcgga gcggataata tcaccgttct tcttatcgaa tataaggagg
    34681 acagtgtatg atcgagttgg gcaaaaaatt aaatggccgt tatcaaatca ctggaaatat
    34741 cggtagcggt ggtatggcaa atgtattttt ggcgcacgat ttaatcttgg atcgtgatgt
    34801 agcagttaaa gtcttacgat ttgactttca aaatgaccag actgcgattc gacgatttca
    34861 gagagaagct ttagcagcaa cagaactcgt tcatccaaac attgttagtg tttacgatgt
    34921 tggtgaagaa gacaacatgc agtatctggt gatggaatac gtcaaaggaa tggacttgaa
    34981 acgatatatc caaacacatt atcctgttcc ttatgaaaca gcagtaaata ttatgcagca
    35041 gatcctttct gctatttctt tagctcataa ccatcaaatc atccaccgag atttgaaacc
    35101 ccaaaacgta ctgatcgata atgaaggtgt tgtaaaaatc acggatttcg ggattgctat
    35161 cgctttatct gaaacatcga tcacgcagac gaatacgatg ctaggatctg ttcattatct
    35221 gtcccctgag caggccagag gaagtatggc aacgaagcag tcagatattt atgctttagg
    35281 gatcatcctt tatgagatgt tgacgggtag cgtaccgttt gatggtgaat ctgctgtaac
    35341 aattgcttta aaacattttc aagatgatct tccatctata aaagcacttg atccaaatgt
    35401 gccacaagca ctggaaaacg tgattttacg ggcaacggct aaagaacctg cagaccgtta
    35461 taagtcagcc gaggaaatgt ctgatgatct ttctacagta ttatcaccag ctcgtgcaaa
    35521 cgaagaaaaa tggcagcctc atgtgatgga taatgaaaca aaagttatca caccccttac
    35581 ggaagatgca ccaatgcctg attcattcaa atctatgcct ctgccaaaag acaagccaga
    35641 aaacgaaacc ttaccagagg aggaagaagt tctgcctgat cctaaaaaga aaggacagaa
    35701 aaaatggtgg ctgatcctat tagctattct tgctgtttta ggaatcggaa cagctgtatt
    35761 ctttgcttct ggcgggcgag gagaagttgt tgttcctgat gtttctgatc tttctgaagc
    35821 ttctgctaga gaaacactcg caaaagcgaa attagaagta gcaacaaaaa cagaagaaat
    35881 tgcagatgac aaaatcgaag aaggtaatgt agtgaagaca gacccggcag cgggaacgac
    35941 agtcaagcaa aaacgcgaag tcactctgta tattagtagc ggcacgaaaa aaatcaaatt
    36001 agatgattac agtggtaaaa gttatgaaga tgccgaagca agtttgaaag aattagggtt
    36061 ttctgctgac ttgattcaaa aagaagagga attcagttca gaggtagaaa gcggaatgat
    36121 catttcgcaa tctccatcag aagatacaga agtcgatcct aaaacagaca cgattacttt
    36181 cacagtcagc aaaggaccag aaacagtgac tttgagtagt catgtaggag aaaactacga
    36241 aactgttcgt aattggcttg ttacacaagg attcaatatc aataatatta gtgctactta
    36301 tgactatagc gacactgtac catcaggttc gatcatcagt caggatccag cctcgggtga
    36361 agttgtagca gaagaaactt acatcaattt tgtcgtaagc caaggaacag agcctaaatt
    36421 gaatgatatc tccggctata caaaaagcga agcacaaagt tatttagcta gtgtcggtgc
    36481 agaatatatc gggcatgaga cttccgaata ttcaaacacg gtggataaag ataaagtgat
    36541 tcgtacagta ccaggggcag gaacgacaat taccaaagga atggttgtga acgtaattta
    36601 ctctaaagga ccagatccag cgtcttctag ttctgaaaaa gactcttcag acagttctag
    36661 cagctcaagt agctccagta gcacaagcaa ctcttcaaaa gaagaatctt ccagctccac
    36721 aagttcatca gctagcacct cagaaagttc aaaataagtg ctgaataaaa tatatcagaa
    36781 gggaatgcga cattgttttg ttgcattctc ttttttgtgt ttctatctct tttgatcaat
    36841 tttcaagcca tatactttga gaagtttgca tggaataaca cctactttcc tcttcgtatg
    36901 gtaaaataaa caaaaacgga ggtggcactt atcaaaggac aaatcagaaa agcaatcagc
    36961 ggtttttact atgtatacgc aaacggagag acctatcaaa caagaggaag aggaaatttc
    37021 cggaatcgga aaatcacgcc gcttgtcgga gatcaggtga tctttgagag tgaaaatcaa
    37081 acagacggct atcttctgga aatcttgccg agaaaaaatc aattagttcg cccaccagtc
    37141 acaaatgttg atcttggaat cattgtcaca agtatcgtag agcctgcatt ttcttataat
    37201 ctacttgacc gctttctagt taccttggaa tacgagtcga tcgaaccgat tatttttttg
    37261 acaaagaccg acttgtcaga cgacccagaa aaaatcgaag agatcaagca aacctatact
    37321 aaaataggct atccggtgat tgtacccaat catacgggtg atacgacaga actgatccgt
    37381 tacttcccag aacggctatc ggtctttatg ggtcagtctg gagcaggaaa atcgacgcta
    37441 ctgaatcaaa tatcaccgga actgaattta gcaacaggag agatttctga tgcacttgga
    37501 agaggacggc atacaacacg tcatgtggaa ctgattccta tctatggcgg gttagtagca
    37561 gatacaccag gatttagctc gattgacttt ttgacaattg aagcaggaga actgccgaga
    37621 caatttcctg aatttgtaga agcttctgtc cattgcaaat ttagagagtg catgcatgct
    37681 aaagaacctg gctgcgaagt aaaacataga gtagaaacag gtggtatcgc acagacacgt
    37741 tacgacaatt atttgcaatt cttacaagaa atcgaaaaaa gaaaaccaat gtatgcaaaa
    37801 aaagaaggta aacgaaaata ggaggaaaca agaatgaaga tagcaccatc cattttgagt
    37861 gcagattttg ccaatttaca aagagacatt gagttagtgg aaaaaggagg cgcagattac
    37921 atccacgtag atgtaatgga tggccaattt gtcccaaata tcacattagg gccaaatatt
    37981 gttcaggcaa tccgtcctgt tactaaatta ccgttagacg tccatctgat gatcgttgat
    38041 ccagaaaact acattccagc atttgcaaaa gctggagcag atatcattac tgtccacgtc
    38101 gaagcaactc ctcatattca tcgggcactt caaatgacaa aaagcttagg agttaaagct
    38161 ggagtagtta tcaatccagg aacaccagtc tccatgatca aacatgtttt gcctttggct
    38221 gatcaagtac tagtcatgac tgtcaatcct gggttcggcg gacaaagctt tattgaagaa
    38281 acagtagaaa aaattacgga actcgctgat ctacgtagag agaacaattg gacttatgca
    38341 atcgaagtag atggcggaat cgttccagaa acagcaaaaa tctgtaaaga tgcaggggcg
    38401 gatgtcttcg ttgcagggtc atatatctat ggagccgaaa atccagtcgg acaaatcgaa
    38461 cgattaaaag aggcattaga ctaaatgaaa attttattag ctgctggagg taatccgcaa
    38521 aactggccgc agtctctgct gtccaaatac gatgcctatg tcgggataga ccgtggtgcg
    38581 ctgtacctgc ttcaagcagg ctacccagta gatctggcag tgggtgattt tgattcattg
    38641 tcgaacgaag agcagaagga agtatttgca aaagccaaag agtatacccg ttcccctgca
    38701 gaaaaagacg atacagatac acagttaggt atcttaaaaa catttgaacg ttacccgcaa
    38761 gcggaagtca ctttgattgg agctacgggc ggaagattgg atcatctttt agccaatctc
    38821 tggttaggta tggaacctcg cttcaaaccc tttgcccatc agcttattat cagagattgt
    38881 caaaattatt tgtcttatta tttgcctgga gagcatatag taagacagat accagatatg
    38941 aagtatttag cttattgctg cttgactccc gtctcgaatc ttacattaaa aggcagcaag
    39001 tatgtattgg aaaataaaga agtagaatat ccgacatctt atgcaagtaa cgaatttctt
    39061 tcagataccg cagaattttc tttttcagat ggtatgattg cagttattca aagcaaagat
    39121 tgatccataa acaaataaaa aaatttgtct ttcaggctaa aaatgacaga atctattatt
    39181 tgctaaaaaa aagcttaaga ggagcaactt accttttgtt tcacggcatt tagtgataaa
    39241 attgaacaca agagtctaat taattcgagg tgtctttgtg aagaagttca atcctaataa
    39301 aaatattatt attaccttgg ttattgttat catcgtggtg acgatactaa gtgtgaccgc
    39361 tgcgcagcga gcgaatgaag gaaagcccaa tttcttccaa tccatcgtta atgacagtgt
    39421 tgcaatggtt gataaaacaa tctctgcccc agtacggtgg gtacgaaatg gagtagattc
    39481 tgtccataat cttttttcaa cttatgccga aaatgagcgg ctaaaagaaa aaatcgatag
    39541 ctatgatgac gtttcacagc aaaacaagaa ttacgagaga gaaatcgaag cgttgaaaaa
    39601 agaacttgag ttgaatcaga cgctgactag ttacgaaaaa gtaacggcaa atgtcattac
    39661 tcgttcgcct gacagttggc aggacatgct gatcgtagac aagggttcta aagacggtat
    39721 cgaagcaaat atggcagtca tgtcgcaaaa aggattgatt ggccgagtga tcgaggtcaa
    39781 tacggcttcg tctaaaatcg aattactgtc atcctctaat gaaagctcca atcattttcc
    39841 agtacgggta tcttcggcta atggcgaagc gtttggtttg cttaaaaact atgatgaaaa
    39901 gctccatgcc ttagtggtga cccaattaac tggtgatacg gatatcaaag aaggggatgt
    39961 tgtccagaca tccggtcttg gagggaattc tccagctaac ttgccgatcg gtacggttat
    40021 taaaacgaaa ccagatagtt atgggctgga tcgggaagtt tatgtgaaac cttatgcaga
    40081 aatgtatgac gtgtcagttg tgacgattgt ccaacggcta gcggaggatt agtccatgtt
    40141 gaagaaagaa acaatgaagt actatctgcc aatcgttttg ttctttttga tgttgataga
    40201 tggtcattta acaagaatgc taggggagtg gtcgaaaggc acctatatgt caaatgccca
    40261 ctttctgata ttggcattat tatgttgcag tatggcgttt gaaaaacgtt atttactgat
    40321 taccacgatt gttctcgggg ctatctatga cgcttactat attggcgtta tcggtatcta
    40381 tgcagtagct ctccctttaa ttgtatggtt gatgtatgta atgaaagacg ttatccatgt
    40441 caacatcttt actgaatttt tcagtatgat catctttgtc acgggttatg aattgtttac
    40501 gatggtggtc cagttgattt ttaaattagc agtagtaaat aacacgtatt ttattacaag
    40561 gtttttagga cctacactgc tgttgaacat gattatattt gtattattca tttttccctt
    40621 taagaaatta ttcagtgatg aataatttct ttttttattt ttaggtcgaa agcgaaaaag
    40681 aatcgttgtt atcttaagaa atgctagact taacgcggct ttttcatgat cattttctga
    40741 aacgaacagt tttgtcattt ttaaaagttg ttttgaaaca tttttgtaac ataactatta
    40801 tatcgttgac acactgctat ggtacaattt ttatatcgtt aagaaagact taaatatatt
    40861 tagatattta ttaaattaag ccagtcggag gaatgaagag tgaaaaagag tttaatatca
    40921 gcagtaatgg tatgttcaat gaccttgact gccgtagcat caccgatcgc tgccgcggcg
    40981 gacgattttg attctcagat acaacagcaa gatcaaaaga ttgcagactt gaaaaatcaa
    41041 caagcagatg cacaatcaca aatcgatgcc ttagaaagcc aagtttctga aatcaacaca
    41101 caagcacaag acctattagc aaaacaagat acattgcgtc aagaatctgc acaattagta
    41161 aaagacattg cagatttaca agaacgtatt gaaaaacgtg aagatacaat ccaaaaacaa
    41221 gcacgcgaag cacaagtaag caacacaagc tcaaactaca tcgatgctgt attgaatgct
    41281 gattcattag cagatgctat tggacgcgtt caagcaatga caacgatggt aaaagcaaac
    41341 aacgacttga tggaacaaca aaaacaagat aaaaaagctg tagaagacaa aaaagctgaa
    41401 aacgatgcta aactaaaaga attagctgaa aaccaagctg ctttggaatc tcaaaaaggc
    41461 gatcttttat ctaaacaagc tgatttgaac gtactgaaaa cttcattagc tgctgaacaa
    41521 gcaactgctg aagacaagaa agctgacttg aaccgtcaaa aagcagaagc tgaagcagag
    41581 caagctcgta ttcgtgaaca acaacgtcta gcagaacaag ctcgtcaaca agctgcacaa
    41641 gaaaaagctg aaaaagaagc acgcgaacaa gcagaggctg aagcacaagc tactcaagct
    41701 tcatctactg cacaaagctc agcaacagaa gaatcatcag ctacacaatc ttcaatgact
    41761 gaagaatcat catcagctac acaatcttca gcaacagaag aatcaacaac acctgaatct
    41821 tcaacagaag aatcaacagc gcctgaatct tcagcaacag aagaatcaac aacagtgcct
    41881 gaatcttcag caacagaaga atcaaaaaca gtgcctgaat cttcaacaac agaagaatca
    41941 acaacacctg cgcctacaac accatcaaca gatcaaagtg ttgatacagg aaacggcaca
    42001 ggaagtagta ctccggctcc aacgccaaca ccaacacctg aacaaccaaa acctgtaaca
    42061 cctgctccag ctccttctgg aagtgtgaat ggtgcagcaa tcgtagcaga agcttacaaa
    42121 tatattggta ctccatatgt ttggggcggt aaagatccaa gtggctttga ctgctcaggg
    42181 ttcacacgct atgtttacat gcaagtaact ggtcgtgaca tcggtggttg gacagttcct
    42241 caagaatcag ctggaacaaa aatttcagtt tctcaagcaa aagctggaga cttgttattc
    42301 tgggggtcac aaggcggaac ttaccacgta gcgattgctt taggcggagg acaatatatc
    42361 catgctcctc aaccaggcga aagcgtaaaa gttggttcag ttcaatggtt tgcccctgac
    42421 tttgctgtca gcatgtaaga atgattaatc aaaaagagtt agcccattct ggactaactc
    42481 tttttt
//
LOCUS       DAFBQH010000016        41307 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.016, whole genome shotgun sequence.
ACCESSION   DAFBQH010000016 DAFBQH010000000
VERSION     DAFBQH010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.016"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(26..328)
                     /locus_tag="LK694_000927"
     CDS             complement(26..328)
                     /locus_tag="LK694_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5051816.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="LK694_000928"
     CDS             complement(353..2446)
                     /locus_tag="LK694_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBK5051817.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="LK694_000929"
     CDS             complement(2935..3699)
                     /locus_tag="LK694_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HBK5051818.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="LK694_000930"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="LK694_000930"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HBK5051819.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="LK694_000931"
     CDS             complement(4704..5780)
                     /locus_tag="LK694_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBK5051820.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="LK694_000932"
     CDS             complement(5804..6397)
                     /locus_tag="LK694_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HBK5051821.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="LK694_000933"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="LK694_000933"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HBK5051822.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="LK694_000934"
     CDS             complement(8067..8366)
                     /locus_tag="LK694_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HBK5051823.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="LK694_000935"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="LK694_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HBK5051824.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="LK694_000936"
     CDS             complement(10121..11032)
                     /locus_tag="LK694_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBK5051825.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="LK694_000937"
     CDS             11152..12009
                     /locus_tag="LK694_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK5051826.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="LK694_000938"
     CDS             complement(12196..12558)
                     /locus_tag="LK694_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBK5051827.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            12904..14343
                     /locus_tag="LK694_000939"
     CDS             12904..14343
                     /locus_tag="LK694_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5051828.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="LK694_000940"
     CDS             complement(14350..16902)
                     /locus_tag="LK694_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBK5051829.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..18347)
                     /locus_tag="LK694_000941"
     CDS             complement(17016..18347)
                     /locus_tag="LK694_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5051830.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18313..19791)
                     /locus_tag="LK694_000942"
     CDS             complement(18313..19791)
                     /locus_tag="LK694_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5051831.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="LK694_000943"
     CDS             complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="LK694_000943"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBK5051832.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22401..23828
                     /locus_tag="LK694_000944"
     CDS             22401..23828
                     /locus_tag="LK694_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5051833.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23920..24285)
                     /locus_tag="LK694_000945"
     CDS             complement(23920..24285)
                     /locus_tag="LK694_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBK5051834.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(24282..24647)
                     /locus_tag="LK694_000946"
     CDS             complement(24282..24647)
                     /locus_tag="LK694_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBK5051835.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24839..25087)
                     /locus_tag="LK694_000947"
     CDS             complement(24839..25087)
                     /locus_tag="LK694_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051836.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25086..25214
                     /locus_tag="LK694_000948"
     CDS             25086..25214
                     /locus_tag="LK694_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HBK5051837.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25271..26965
                     /locus_tag="LK694_000949"
     CDS             25271..26965
                     /locus_tag="LK694_000949"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HBK5051838.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            26969..27088
                     /locus_tag="LK694_000950"
                     /pseudo
     CDS             26969..27088
                     /locus_tag="LK694_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            27065..27361
                     /locus_tag="LK694_000951"
     CDS             27065..27361
                     /locus_tag="LK694_000951"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051839.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFLTWLDLLPQMQA
                     FEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLPRGYEKGV"
     gene            27739..28872
                     /locus_tag="LK694_000952"
     CDS             27739..28872
                     /locus_tag="LK694_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBK5051840.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28903..29223
                     /locus_tag="LK694_000953"
     CDS             28903..29223
                     /locus_tag="LK694_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HBK5051841.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29252..29716)
                     /locus_tag="LK694_000954"
     CDS             complement(29252..29716)
                     /locus_tag="LK694_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5051842.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29847..31104)
                     /locus_tag="LK694_000955"
                     /pseudo
     CDS             complement(29847..31104)
                     /locus_tag="LK694_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(31133..31450)
                     /locus_tag="LK694_000956"
     CDS             complement(31133..31450)
                     /locus_tag="LK694_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBK5051843.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(31476..31796)
                     /locus_tag="LK694_000957"
     CDS             complement(31476..31796)
                     /locus_tag="LK694_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5051844.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(32260..33675)
                     /locus_tag="LK694_000958"
     CDS             complement(32260..33675)
                     /locus_tag="LK694_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK5051845.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIYLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(33692..35008)
                     /locus_tag="LK694_000959"
     CDS             complement(33692..35008)
                     /locus_tag="LK694_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5051846.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(35158..35856)
                     /locus_tag="LK694_000960"
     CDS             complement(35158..35856)
                     /locus_tag="LK694_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBK5051847.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(36145..37101)
                     /locus_tag="LK694_000961"
     CDS             complement(36145..37101)
                     /locus_tag="LK694_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK5051848.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(37214..38047)
                     /gene="msrA"
                     /locus_tag="LK694_000962"
     CDS             complement(37214..38047)
                     /gene="msrA"
                     /locus_tag="LK694_000962"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBK5051849.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(38222..39856)
                     /locus_tag="LK694_000963"
     CDS             complement(38222..39856)
                     /locus_tag="LK694_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051850.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(39843..41249)
                     /locus_tag="LK694_000964"
     CDS             complement(39843..41249)
                     /locus_tag="LK694_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051851.1"
                     /translation="MDFFIYTKYFILSSFYYGEKFFKKIDAAFLFSCLYTFATYRTVD
                     IYYRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSAL
                     IACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFK
                     TDLQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVS
                     TWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLII
                     GATVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKE
                     RPHLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVV
                     NDSYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKL
                     KDSRYVTKKEINKKNENK"
BASE COUNT        12552 a         8488 c         6973 g        13294 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    17221 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    17281 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    17341 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    17401 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    17461 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    17521 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    17581 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    17641 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    17701 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    17761 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    17821 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    17881 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    17941 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    18001 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    18061 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    18121 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    18181 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    18241 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    18301 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    18361 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    18421 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    18481 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    18541 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    18601 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    18661 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    18721 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    18781 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    18841 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    18901 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    18961 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    19021 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    19081 tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat
    19141 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    19201 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    19261 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    19321 taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt
    19381 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    19441 cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc
    19501 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    19561 attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga
    19621 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    19681 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    19741 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    19801 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    19861 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    19921 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    19981 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    20041 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    20101 ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga
    20161 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    20221 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    20281 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    20341 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    20401 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    20461 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    20521 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    20581 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    20641 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    20701 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    20761 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    20821 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    20881 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    20941 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    21001 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    21061 ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg
    21121 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    21181 gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    21241 ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    21301 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    21361 aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag
    21421 ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac
    21481 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    21541 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    21601 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    21661 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    21721 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    21781 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    21841 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    21901 taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    21961 tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga
    22021 attttttttt tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg
    22081 gatttttctg ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa
    22141 aatagataac ctaaaaaaag cgaacaaaca aataaaatga ctgtccttca ttcactaaac
    22201 atatttttat ttttttatat tgttaaaaat tcgcaattta ttttttaata gaattatctg
    22261 ttttccaaat tttataacat aaaaaacctt ttttaacggc atttatatgc tttttcagaa
    22321 attaactgat gatttcaaaa agaaaacatg tcataatatt tcccatattg tgcaaaaaaa
    22381 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
    22441 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
    22501 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
    22561 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
    22621 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
    22681 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
    22741 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
    22801 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
    22861 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
    22921 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
    22981 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
    23041 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
    23101 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
    23161 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
    23221 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
    23281 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
    23341 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
    23401 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
    23461 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
    23521 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
    23581 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
    23641 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
    23701 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
    23761 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
    23821 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
    23881 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
    23941 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
    24001 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
    24061 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
    24121 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
    24181 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
    24241 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
    24301 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
    24361 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
    24421 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
    24481 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
    24541 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
    24601 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
    24661 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
    24721 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
    24781 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
    24841 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
    24901 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
    24961 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
    25021 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
    25081 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
    25141 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
    25201 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
    25261 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
    25321 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
    25381 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
    25441 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
    25501 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
    25561 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
    25621 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
    25681 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
    25741 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
    25801 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
    25861 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
    25921 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
    25981 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
    26041 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
    26101 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
    26161 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
    26221 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
    26281 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
    26341 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
    26401 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctatcgt
    26461 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
    26521 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
    26581 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
    26641 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
    26701 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
    26761 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
    26821 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
    26881 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
    26941 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
    27001 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
    27061 gcagatgcaa gcttttgaac accgtttacc aagaggttgt gaaaaagctg ccctgcatca
    27121 agtaataaga acacccaagc taaaactgct tcttatgttt ttgacttggt tggacttatt
    27181 accgcagatg caagcttttg aacaccattt accaagaggt tatgaaaaag ctgccctgca
    27241 tcaagtaata agagcagcca agctaaaaca gcttcttatg tttttgactt ggttagactt
    27301 attaccgcag atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa aaggcgttta
    27361 gtttcgagta tgaagaaaaa ttttgtgaaa atggttcctc acgtttttgc ataaatttga
    27421 cttaataccg agaaactgat cccttctgtc ataacctctt tttatttatg tctgtttctt
    27481 tttgaaaaca actatttttt caacgtttac tctcttgcca atccaacatc atctaacgga
    27541 tacaactatt tcaatgatta ccattacact aaaaaatcgg aaacacacct ctccgaacac
    27601 aaatacataa gactatagaa tgtttcattt aagaaaaaaa gcgtttcatt atcaaaaagg
    27661 taagatctcc tttaaaatga aattatctgt agtactaata atgcagacaa ttttatgaat
    27721 ctgaaaggag catctccaat gaaagcagtc acttggcaag gaaaacagaa aatggaaatc
    27781 agaaacgtag atgatccaaa aatattagaa ccaacagatg caatcatccg tatcacagca
    27841 acagccattt gcggctccga tctgcatctt taccatcatg gtgaagcagt tttggaaaaa
    27901 gggtatgttg taggacacga accaatggga atcgttgaag aagtgggatc tcaagttcaa
    27961 acattgaaaa aagggcaacg tgtggttatc ccttttaata ttagttgtgg tcattgtcat
    28021 ttctgcctga atcacatgga aagccaatgt gatgaatcaa atccagatca gttatatggc
    28081 gggcttttcg gatttggcaa gttgaacggt aatcattggg gtggtcaagc ggaatatcta
    28141 agagttcctt atgcagactc tacttcattt gttgtaccag ataatgatct tcctgatgaa
    28201 aaagttttat ttctatcaga tattttgccc acagcttatt ggagcgttga aaacgcagga
    28261 gtaaaaaaag gcgatacagt tgtgattcta ggttctggtc cagtcggtct attcgctcaa
    28321 aaattcgctg ttatggctgg agcagagcgc gtaatcgcag tagatccagt acagcaccgt
    28381 ctagataaag cggctaatta caatggggtt gaaacttatt tcttagaaga cacggcacaa
    28441 gctggaacag aattgtatga actggctaaa ggtggcgcag atgtcattat cgattgtgta
    28501 ggaatggacg gattagagcc tgtcaaagaa aaagcgaaaa atttagtaag cttacaaagc
    28561 ggcacaatct ctccattaca gatggcatcg caagctgtca aaaaatttgg aacagtccaa
    28621 gtaactggtg tttatatgac tccagcttct tcttacccgc tgcaagaatt ttttatgcgg
    28681 gatatcgctg tcaagcatgg acaggcacca attatccatc taatgccaaa aatttacgca
    28741 atgattgcag aaggactatt cgatcctaca caaatcatta ctcattcgct gccactatca
    28801 gaagcagcac aagcatatga gatctttgac aaaaaagaag ataaaaatat aaaagttgtt
    28861 ttgaaacctt gaattcagta aaaaatacag gtgattttct ttatgcccaa aatcaaacga
    28921 aatacccatg agatcgaaat aggaaaagaa gaagatatcg aaatacgagg acgtcgcttc
    28981 cgcttctatt tccaaaatcg ttacacattg atctcactgg ctgtcgattt attaaccggg
    29041 attttctata tttttggaag ccttgcttcc ttaactaaaa tccccgatcg atatggcacc
    29101 tatttttatt tggctggtgc tattttcttg acgattcgtc ctattctcag aatcgtaagg
    29161 aacattttta tttacgacga aaaaaaagca atgaagagaa caagcaaaaa aaaagaggca
    29221 tgagaatttt tcaaatctca tgcctctttt ttcaccctat cgtatcactt tgatacagac
    29281 gagcggtcgt cccactttct tccttgacca ttgtcaaaaa tgtccactcg tatcgttcat
    29341 aaaacgtggt atgatctgta attaagtaaa gtttttcgta cccaagttga tgcatttctt
    29401 ttctgatttc cgctaatatc cgcttggcta tcccctgctt acgcttttcc ggttcaacaa
    29461 acagggcaca aagatttggt gtcagatcag ggcggttatg gaagtcgttt tcaatgacgc
    29521 ccgctcctcc gacgatggtt tctttttcat ctaagaccac ataccattga ggaatacttt
    29581 ccttattagc gattccagta cgaatacttt cctcatacac aacagcagga actccccatt
    29641 ttgatgaaaa ccaattcgcg ctttgtttta ctaattctgg gtgttctctt aatttgatcc
    29701 agttatatgt aaccaatgtt taaacctccg actaaaataa aacgtttaca aaaattatac
    29761 aagtctttat gaaaattagc aatgacaaca atagtgatgt aaaaaaaaaa cggaaaacaa
    29821 tcacttgttc tccgtttttt gattaatcaa gcatctaaag cttcttttct tctttcttgg
    29881 atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa attgacagcg
    29941 gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat tggtggagtt
    30001 acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc aaagtaagca
    30061 acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg gttcaacacg
    30121 ataggtaacc caaacatcgt tggttcatta atattgaaga cacctggcgc aatcgacaag
    30181 tttgttacaa ccatatatgg tttgttttta cgtcctacta aataaatagc taacagtaaa
    30241 cctaaagttg cacctgttcc tcctaagttg acaaagctgt caaagaaagg tttgttcacg
    30301 ataaacggtg cgctatgccc agctgcgatt gcattaacgt ttgcttcaat ggctggcgca
    30361 ttgattgttt gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa gaaccataag
    30421 aatggtgtga taaatgcaag cagtaaggca gatggataag agtttgcaag tcccataaat
    30481 ggttcttgga ccactgtata gaaagaacct acgatatcat caacaccgaa tgcagcaaaa
    30541 attgcagcaa ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa tgaagcaaat
    30601 gcttttgcaa ctgccggtgg cacaccatca ggcattttga tgatcaattt ttcatttccg
    30661 cttaaacgta caaagatttc tgttgaaata atcccaacaa taatagcaat aaacaaaccg
    30721 tttgagctca tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga aatcacacct
    30781 gcggaaacac cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa gcaagaaagc
    30841 aatcaagaca gaaagaatgg caaatgtacc gttccaagca gcagccccga atcctttcca
    30901 tgattcacca ccaaaaattg agttcatcaa ttcttggaac ccgggaatcg gtaagttatt
    30961 gatcattact gctaatgctc ctaagatcat taacggcata ctcaccataa atgagtcccg
    31021 aatcgcaact aaatgccgtt gtgcaccaat ttttgatgct acaggtataa aatgtttctc
    31081 cataaatgca acaaatttat ccataaatct tccctctttt ctttactgta gattacgcgt
    31141 tcaatcgacg atacagatca atgatctctt ttgctaaatc tgtaaaagct atagatgtca
    31201 tcagatgatc ttgactgtgg accgtcaaaa gcgtgacttc aacgtgattt ccttgtgctt
    31261 cttgtgtcag catctctgtt tgagagtgat gtgcttggac taaagattcc tctgcatctt
    31321 tgattttttc atctgctaaa gtgaaatcac cttttttcgc agcagcaatc gcttccattg
    31381 catcgctttt tgcatttccg ccaaacatga tcagacccat gatggcttct agattttttt
    31441 gatcttccat ttattgtatc ctccgataat ttttattatt tgtttattag tttttctgct
    31501 tggtcgagta ctttttcacc attcatcatg ccatagtcag ccatattgat tacatctaaa
    31561 ggaatgcctt taggtgctag cttttcagca aattgatttt gcataaagcg tacttgcggt
    31621 cctaaaagta aaacgtctac atttttagat tctagttgat tatccgcatc tgaagcagat
    31681 acagcaaaga tgtctgcttc cattcctttt gcttctgctg ccttttgcat ttttgtgact
    31741 aggagacttg tactcattcc agctgaacat acaagcatga ttgttttctt ttccatttta
    31801 aacacgttcc tttctatact tacacttgta tataagcaag tagcgtgcca actttaaaag
    31861 cgctttaaaa agttgatatc atcacattta aggtctatac caagttgcat acacactaaa
    31921 aaagaaagcg cacactaaac acacaaatta atttttatta ccttttttct tttaacgtgc
    31981 acaaaaaaag ttgtgacaca aaacttgtca caactttttt caaataaacg gtattcaaaa
    32041 gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca taagatgttg tttttacttt
    32101 gctattctta ttacttgatg caggacagct ttttcacaac ctctcaataa acggtgttca
    32161 atcagctgac cctgggtatt agctcaaaac gctggattca caacctcttt tatttcaaat
    32221 aaccagtaaa tacaatgaac atctacaaaa aacttctatt tatttataga tttcgtcagg
    32281 caaatccaat tctctcttat caatcaaagt ctgataccac gaagcagatt ttttcaatgt
    32341 tctgcttcgt tcatcgttca attcgattcg aactaatcca tatcgattct taaatgcatt
    32401 cattggtgac acacagtcag tgaatgccca taacatatag ccttcgcaat ttgcgccttc
    32461 ttctgttgct ttgatcaagt aatgcaaatg ctgtccgata aattcgatac ggtaatcatc
    32521 ttgcacgatt cctttctcat ccatatactg ttcttcatat tctcttccca taccgttttc
    32581 tgttatcagc caagggatat tatgataatt ttcttttaga tacatggcta tatcatacat
    32641 gattttcgga tagatttccc atcccctgga attattcatt ttcttcccag gtaaatcaaa
    32701 gacttcataa tatcgttcag gatggaaagg tgtctcttca ttccattgat aacgaggggc
    32761 gcgaacgcgt ttaggataat attgattgat tccaaggaaa tctactgtat gatgctgaat
    32821 cgtttctaaa tcctctgttg caggatcgaa ataaatcgat tgtttttgac acaactccag
    32881 gaactcttct gtgtagtatc cttttaccat cggatcaaaa aatactcgat tataaaacaa
    32941 atcatacata taagcagctt tttggtcttc tggagaattg gatcgagcat agaccatttc
    33001 aggatttaaa atacagccaa tccttccacc ataatttccg ctgcggtaaa tctctactgc
    33061 tttagccgtt gctaaaactt tatgataatt ccacatcatc cacttttttg tgttttgttc
    33121 atgcggccag cgaacagcat ccaaatagca acgcgtttgg ggaacgattg gttcgttgaa
    33181 tgtaaaccag ttttttaccc tatcaccgta acgatcaaag gcaatttgag catacttagc
    33241 ataaaaatca actacttttc ttgaagacca gccatcatat ttttccaaaa gaaaagctgg
    33301 gacttcataa tgttccaaac aaatcatcgg ttctacgtct gcttcgatca aagagtcgat
    33361 catttcactt atatgctgag catattcttc atctactata cagttctcat aatcagtaaa
    33421 aaaacgagcc caattgatag aagtccgata atgtgtcaga tagatggatt tcatcagatc
    33481 cacatcctgc tggtattgtt ccataaaatt agttgctacg gctgggccat acccattatg
    33541 ccaaacaaaa tgttcatttt tataccagct atctataaac gaatcctgtc cttctttttt
    33601 tcctttccaa ccttccgttt gccaagcaga agatgcagca ccaagaataa aattttttgg
    33661 aatagtaatt ttcatgattt cctcctgttt tttagatccc atcattttcc acacgatttg
    33721 ctacttttac aaatggcaga tagatcaaaa tagaaacaat aatacaaaca agttgcgtaa
    33781 taactgcacc gatatttcct gctgttgcca gataagcatt gacgatcggc ggcattggcc
    33841 aagggaccat tacaactgct ttcgccgcaa atcctaaagc ggttgcaaaa taaccaatcg
    33901 ttccagtgat aagaggcgtt aagataaacg gaatagctaa aattggattc agcatgatcg
    33961 gcataccaaa aataactggt tcattgatgt tgaagatccc aggagcaagg gataacttgg
    34021 tgatttgttt catatcttcc cgttttccaa ctaacaatac tgcaatcagt aaaccgatcg
    34081 tgactccaga cccgcccata ctcatataca tatcccaaaa tggcatagta ataatgttcg
    34141 gtacttcttt tccagcttca aaagcttccg tatttacagc aattgccgct aaaagcaacg
    34201 gttcccgtat cggcttcacc atttgatttc catggatacc aatcacccaa aagacttgcg
    34261 caataaacat caatagcaaa atacccggta acccttgtac gacaccttct aatgggcgtt
    34321 gtacgacatt gtaaatgata tcataaaggt acattcctgt aagttctttg atgataaatc
    34381 ccgcagccgc gatacccgat acagtcagaa tcgtcgggaa taaagcagaa aaactagcag
    34441 aaacattcgt tggcacggta tcaggcattc tcagctgtag tttcttctgt ttcctcaacc
    34501 agcaataaag ttcaatagaa aggatggaga ccaacattcc caaaaataac cctttcgtat
    34561 ctgtatattg tttagccaat acgttttcta ctataacagt ctgattttca ttcactaatt
    34621 cataaacagt tggattaaca gataaataag acatcactgc gatgatacca gggaatacac
    34681 catccacttt attcagcttg gctaattcga tccctatcag aaaaacagca tagatggtca
    34741 agaaacttag tgtgacatag ctgatggcag atgcgatagg ctttaattct tctaaaaaac
    34801 gcaagaaact tatctgtgct aagccatttt ccgagtcaaa gaccattgct gaaaacaatg
    34861 tgccaaaagc acccgtaata attaccggaa tcaatgcagc aaatgcgttt tttatcacca
    34921 tgatatacct taaattattg attctcgttg ccacgttccc taaaacattg gctaatttat
    34981 ccataaaact cattccagat cccctcattc ctgcttcttc tccttgacgt catctctttt
    35041 tgtctattag tcagcgctta catttcgatg aacagaaact atttactcct cttttttacc
    35101 aaatataaaa caaaaattca tctgatgtat ttatcataaa atacatcaga tgaactgtca
    35161 attagtttct ttttctccta ttattttctg taattctctc cgatttattg ccatatggac
    35221 agtcattgca tcaaacgcag caagtgcatt ccgcctttcg atggcctgaa cgatctctgt
    35281 atgaatatcg atcgtctcga ttttcagatg ctggatatca taatttttat taaacaaagc
    35341 aattgattca ttaatgattg ggataatatg cagcaaggct acgtttccgc tagcttctgc
    35401 aatcatcgta tgaaaatcga tgtctaattg aacatgttct tgtgtgcctt ctcgaaaaga
    35461 agtttcaatc ttttgcgtta attttttcat ttggacgatt tgattcttcg ttgcatattc
    35521 cgctgccaaa cctgccatct taggctccaa taaatatctc agttcaaata agtctcgtat
    35581 catccgatcc tgatcttcaa tcaaagaaaa accgagtggg tcattggcaa tccctcgctt
    35641 atcactgaca aatgtccccg ctccttgccg aacttcaagg atattcctgg aagctagagc
    35701 ccgcacagct tctcgcaccg tgcttcgccc cacctctaaa taatcggaca attcatattc
    35761 attcggcagt ttatctccta cttgcaactg catttccttt aaatatctaa ggatcttgtc
    35821 agtagttact tctactaggg ttttattttc ttccaaacca catacctcct tttcatctga
    35881 tgtatttttt cgtaattatt ataacacgtt tgaatcatct gatgattttt tgtatacaaa
    35941 attgattaat ccgttcatag acatacaaac gaagaagata tttaccataa aaaagactag
    36001 gataaaattc actcctagcc ttcgatcatt tttattttga acgacaaaaa atatgatgtt
    36061 caataacaga cgatcttcat tcgattcttg atgtcatctt ttgttttttt taatatatgg
    36121 ataaaaagtt tgattacatc gtttctattc tagaatttgg tcgattctca attcttcttc
    36181 tggtgagaaa tctaaattat ccatcattcg aacactttct tgtaactgac ttaaccgact
    36241 tgctccgacc agtacacttg taacagattt ttgtcgcaaa ttccatgcaa gggccatttg
    36301 cgctaatgat tgtcctcgtg atacggcgat tgtttgcagt gctttgattt tttccagagt
    36361 tgatccaact tgttcttctg aaaggaatgg tatttcctta cgatgtgctc tagaatcttc
    36421 aggaatacca tgtagataac gatttgtcag caacccttga gcaagcggac tgaacgtgat
    36481 tgcccctagc ccttcttctt ctaatactgg agatagatcg tcttctagta gtgcacgaga
    36541 aaacatattg tagcgcatct gatgaataat gaacggtgct tcctttcttt ttaaaatttc
    36601 agtcattttt tttgtatctt ctccattata gttggatata ccaatatata atgcttttcc
    36661 ttgtcttaca agttgcatca aagcttctgc tgtttcttcc aaaggtgtat cagggtctgg
    36721 gcgatgatga tagaagatgt caacataatc tagccccatt ctttggagac tttggtcaca
    36781 acttgaaatg atggatttac gagagcccca ttccccatat ggcccttccc acatatgata
    36841 acctgctttg ctagaaataa tcagttcatc acggtaggct tgaaaatctt ctctaaaaat
    36901 acgcccaaag tttttctctg cagctccagc agggcctcca taattattcg caaggtcgaa
    36961 atgggtaatt cccatgtcga atgcgcctct taaaatctct ctttgattat ggataggatc
    37021 ataatcacca aaattttgcc ataatccgag agataaaatc ggtaatttca aacctgaatg
    37081 accggattta cgatattcca ttttttcata acgtgcacta tctgcaatat acaatttttc
    37141 tcctcctcat caaatctttt taaaacgctt tcttttttct atttttagtt tactacattt
    37201 taaagaaaag gagttaatca aaatgatttt ttcgtatatt tttttgccat ttgtttttta
    37261 ctcgttgata gatcagaaat tgattacgtg cttgctggat ttttttataa cgtttcggtt
    37321 gttttttgta aaattgctga tgatagtttt ctgctggcca aaacgtaccg gctggttcga
    37381 tttttgtgac gattggttgg tggaaagttc ctgaactgtc tagtttctgt tttgattgtt
    37441 ccgctaattc tttttgtcgt tcgccttgat aaaaaatcac tggacgatat tgcttccctc
    37501 tatcttgaaa ttgtccaaat gtatctgtgg gatcagtaat ttgccaatag attgtcagca
    37561 actcgctata acttatttct cttgtgtcat aaatgatctc cacagcttcc acatgacctg
    37621 tgtaaccacc acttacttga tcataatttg gtttttcaac atgtccgccc gtgtatcctg
    37681 acaaaacaga taggatccct tttttcgatt caaacggctc gaccatacac cagaaacatc
    37741 cccctgcaaa tactgcacgt tcttgatagt cagcatcttg catggcatgc ttggaaaagt
    37801 catatttttg ctccttatca aatccttccg taatcaataa ataaaagtcc attacatcag
    37861 gcgtcagatt cattcggata gctaatggac gtagtgttgc ttctagtttt gataactgtt
    37921 ctttaacgga tatattttct cttatgtcat tctttgtctg aatcaatact tttcgttccc
    37981 aatctcgtgt attcggattc aaaatcagat tataaagtgt ttgaatgaat atttgttcct
    38041 catccataga ttctcctcct ttccgaagaa gacaagctag tgactttagc tgtaatttat
    38101 cataaagtga ggaaataagc gattacttca tccccctaca gatttttcgt ataaaaaaga
    38161 gagaatcctt atcgacagat tctccttttt aatcaattta ttttataaat gaaaagttaa
    38221 tttacctttt atggaaaact attttaaaaa ataaacataa atatgcagtt aatgtaacaa
    38281 gataagaaat aatagtcgtt actgtataac ttgtgcttac tttaatatca gcaactcctt
    38341 tgactccgct tatttgtacc aatccattag cattcacctg ctcctttaca ggttgatgat
    38401 ttatttctac attcgttcct aaataagcat atacaggaag tgtgattact tggctcttct
    38461 cattagttac tctatatatg atagaactac cagttgtttt ataaggaagg tttacccatt
    38521 gactgccaat ctttgcttcg tgttttatta tatgctcctt attctccaat gaagcgatag
    38581 gagaataatc atataaaaaa taatccattc ctttagtggt atacgcctct actgtttctt
    38641 tgtttacaac aatatgtccc ggctgagaaa ttattttttg attgaaattt tttttactag
    38701 aaacagttaa agtgattaat aacaaaacag tcataaataa ataaaatagt tctcttgtct
    38761 tattgtatct tctatttttg aaaaatatca tcgttaatat cagagaagag aatattattt
    38821 gcaagcttaa gatccgccaa ggaaattgta acaatttggc tggcgtattt tgcagaagat
    38881 accatggcac caaactagtt gtagctagcc aagtcaaaca cgtaccataa taaatctttt
    38941 tttctatgtt tgttaatttt ttaaataaaa tcaggggaat acataaagat aacagaccaa
    39001 ctagtcccat tgagtaagta gttaagtcgt tgttcatagc ttcagtcaga ctatcgaaca
    39061 cattagatgc atgagcatat aaatttgtca cgctaggttt gttgattttt tggtagagca
    39121 tttgttcaaa catcggatac caaaaagcac tcgtcagaac caatgtcatt cctgccgcac
    39181 taaataattt tttaaatata gcaataaaat ctgcattctt gaaaccttta gaaaccaaac
    39241 gaataaaaat aaaaatgata attaatgctg tactcattaa tgttgatagt acatggctgt
    39301 acactagcaa actcatactc aatgctagct ttacagacgg atacttttct tcttttccag
    39361 agatgatttg ataagcataa aataatacag ggataacaag tgttattgcg atagcttcgg
    39421 caattgcgga acgatagtag atgtctactg tccgataagc agagaaaata tagagacaac
    39481 taaataaaaa tgccgctttt ttttgtttta aaaaccgctc gccataatga taagctacga
    39541 gtgttgtaca caagttcaat aaccaaacgt aaataatata gcttacaatc aaattttttg
    39601 tcatccaata taacattaca gctggaaaaa aagtcagaaa gggataaaaa taatttacac
    39661 catatccagt ataattgaat gtctcataat taatcggact ggtaaaaata gtcgataacc
    39721 ccttgattcg agataagtga aaagaaagat ctccagtggt ataaatactc tgtgtatttt
    39781 tgaaaaagat gaaagaatat atactactaa ctataattaa tccactcaat attactactt
    39841 ttttatttat tttcattctt cttgtttatc tcctttttag tcacatatcg agaatctttc
    39901 aacttattag gtgcaaatcg tttaaaactt gaatacacca ctaaacaata agtcagaata
    39961 ctgatccata aagccatgag agcagttgga gtatattttg ttgtaatttc tatcttattt
    40021 tctccagcat ttacatccac attcactaag ccatgttttt tataagagtc atttacgact
    40081 ttaccgttta cttttacctg tgtacctaaa taagcatata ctggtactgt cactttttgc
    40141 tttttgttgc ttgccactgt gtaagtaatg ttattatctg cagctttata cttccctttt
    40201 tcccacgatt ctcctacttt tacttcatgt ttttctaagt gaggcctttc ttttatcgct
    40261 tctgttgggg cgtaatcgta taatcctccc atatctgatg ttgtataacg tgggagtgtt
    40321 tcttcattga taacaatatg accgtgtttt gattggataa cttttgcgta attttctttt
    40381 gctgctatag acgtgacgag tagaaacacc acagtagcgc caatgattaa ccatgaatat
    40441 tttttattca tagtcttttt cataaaaaca agcgttatga tcattgaaga aaaaaagatt
    40501 tggataccta agatacgcca aggaaactgg agcaagctta tcggtgtttt ttgaaacaaa
    40561 aaccatggaa ataaattagt tacgcatacc catgtagaaa ctacgccaac atagatgatt
    40621 ttttcttttt tctccatttc tttcagtatg aataaaggaa aaatcaacgc aaccaatcct
    40681 aataacccca tagtgtatgt tgtaatatcg ttatttaacg cttgaatgat actatctgaa
    40741 atagaaagtg cttctagctg caaatctgtt ttgaagggac tgtgtatttc ttgataattc
    40801 atctgctgca acataggata ccaaaaatag ctagttaatg cacaagtcaa aaaaacagcc
    40861 tttactaaat tcacgaccac aaattttata tcgtttttta tttgcttgct taaaaacaaa
    40921 cgaactataa tcatacaagc aatcaatgca ctacacaata gtgtagttaa gacatgagta
    40981 tacacaagta gacttatact gacagataaa gcgatgactt ttttttctct attgaatatc
    41041 aatccataag cataatataa aacagctggg ataatcgtaa gagcgattgc ttctgcaatt
    41101 gcggatcgat aatagatatc aactgttcga taagttgcaa aagtatataa acaactaaat
    41161 aaaaaagcag catctatctt tttaaaaaac ttttctccat aataaaaact agagagtata
    41221 aaatattttg tgtaaatgaa aaaatccata caaaaaagga agtcccttct gtagaataaa
    41281 gttaacgaca accaattcac agaaaag
//
LOCUS       DAFBQH010000017        39958 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.017, whole genome shotgun sequence.
ACCESSION   DAFBQH010000017 DAFBQH010000000
VERSION     DAFBQH010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.017"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="LK694_000965"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="LK694_000965"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBK5051852.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="LK694_000966"
     CDS             1011..2258
                     /locus_tag="LK694_000966"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBK5051853.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="LK694_000967"
     CDS             2334..2678
                     /locus_tag="LK694_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051854.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="LK694_000968"
     CDS             complement(2795..3121)
                     /locus_tag="LK694_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051855.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3494..4696)
                     /locus_tag="LK694_000969"
     CDS             complement(3494..4696)
                     /locus_tag="LK694_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5051856.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4813..5328
                     /locus_tag="LK694_000970"
     CDS             4813..5328
                     /locus_tag="LK694_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK5051857.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5527..5973)
                     /locus_tag="LK694_000971"
     CDS             complement(5527..5973)
                     /locus_tag="LK694_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBK5051858.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6020..7498)
                     /gene="msr(C)"
                     /locus_tag="LK694_000972"
     CDS             complement(6020..7498)
                     /gene="msr(C)"
                     /locus_tag="LK694_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBK5051859.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7852..8481)
                     /locus_tag="LK694_000973"
     CDS             complement(7852..8481)
                     /locus_tag="LK694_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBK5051860.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8465..8929)
                     /locus_tag="LK694_000974"
     CDS             complement(8465..8929)
                     /locus_tag="LK694_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK5051861.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9416..10684)
                     /locus_tag="LK694_000975"
     CDS             complement(9416..10684)
                     /locus_tag="LK694_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBK5051862.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10915..11652)
                     /locus_tag="LK694_000976"
     CDS             complement(10915..11652)
                     /locus_tag="LK694_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK5051863.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11719..12288)
                     /locus_tag="LK694_000977"
     CDS             complement(11719..12288)
                     /locus_tag="LK694_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBK5051864.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12416..13288
                     /locus_tag="LK694_000978"
     CDS             12416..13288
                     /locus_tag="LK694_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK5051865.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13630..14502
                     /locus_tag="LK694_000979"
     CDS             13630..14502
                     /locus_tag="LK694_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK5051866.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14499..16457
                     /locus_tag="LK694_000980"
     CDS             14499..16457
                     /locus_tag="LK694_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK5051867.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16708..17559)
                     /locus_tag="LK694_000981"
     CDS             complement(16708..17559)
                     /locus_tag="LK694_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK5051868.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17682..18149
                     /locus_tag="LK694_000982"
     CDS             17682..18149
                     /locus_tag="LK694_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBK5051869.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18412..18894)
                     /locus_tag="LK694_000983"
     CDS             complement(18412..18894)
                     /locus_tag="LK694_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBK5051870.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18994..19857)
                     /locus_tag="LK694_000984"
     CDS             complement(18994..19857)
                     /locus_tag="LK694_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBK5051871.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            20455..21303
                     /locus_tag="LK694_000985"
     CDS             20455..21303
                     /locus_tag="LK694_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051872.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(21474..21815)
                     /locus_tag="LK694_000986"
     CDS             complement(21474..21815)
                     /locus_tag="LK694_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HBK5051873.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            21901..22608
                     /locus_tag="LK694_000987"
     CDS             21901..22608
                     /locus_tag="LK694_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBK5051874.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            22595..22918
                     /locus_tag="LK694_000988"
     CDS             22595..22918
                     /locus_tag="LK694_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBK5051875.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(23007..24044)
                     /locus_tag="LK694_000989"
     CDS             complement(23007..24044)
                     /locus_tag="LK694_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBK5051876.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(24491..25474)
                     /locus_tag="LK694_000990"
     CDS             complement(24491..25474)
                     /locus_tag="LK694_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBK5051877.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(25890..26660)
                     /locus_tag="LK694_000991"
     CDS             complement(25890..26660)
                     /locus_tag="LK694_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5051878.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(26653..27588)
                     /locus_tag="LK694_000992"
     CDS             complement(26653..27588)
                     /locus_tag="LK694_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5051879.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(28182..29831)
                     /locus_tag="LK694_000993"
     CDS             complement(28182..29831)
                     /locus_tag="LK694_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBK5051880.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(30026..30922)
                     /locus_tag="LK694_000994"
     CDS             complement(30026..30922)
                     /locus_tag="LK694_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK5051881.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(30915..31595)
                     /locus_tag="LK694_000995"
     CDS             complement(30915..31595)
                     /locus_tag="LK694_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5051882.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(31596..32378)
                     /locus_tag="LK694_000996"
     CDS             complement(31596..32378)
                     /locus_tag="LK694_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5051883.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(32371..33288)
                     /locus_tag="LK694_000997"
     CDS             complement(32371..33288)
                     /locus_tag="LK694_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5051884.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(33291..33575)
                     /locus_tag="LK694_000998"
     CDS             complement(33291..33575)
                     /locus_tag="LK694_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051885.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            34363..37590
                     /gene="fss3"
                     /locus_tag="LK694_000999"
     CDS             34363..37590
                     /gene="fss3"
                     /locus_tag="LK694_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBK5051886.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            37703..38017
                     /locus_tag="LK694_001000"
     CDS             37703..38017
                     /locus_tag="LK694_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK5051887.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            38030..38404
                     /locus_tag="LK694_001001"
     CDS             38030..38404
                     /locus_tag="LK694_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK5051888.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            38414..38749
                     /locus_tag="LK694_001002"
     CDS             38414..38749
                     /locus_tag="LK694_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HBK5051889.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            38832..>39958
                     /locus_tag="LK694_001003"
     CDS             38832..>39958
                     /locus_tag="LK694_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBK5051890.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRD"
BASE COUNT        12155 a         8142 c         7384 g        12277 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgac cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
     3421 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
     3481 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
     3541 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
     3601 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
     3661 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
     3721 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
     3781 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
     3841 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
     3901 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
     3961 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
     4021 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
     4081 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
     4141 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
     4201 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
     4261 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
     4321 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
     4381 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gtttttttca
     4441 aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg actaacatgt
     4501 aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt
     4561 ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta gctgctacaa
     4621 ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata tctatttgat
     4681 tctccaatat ttccatccct ttctctctat tagcatcata catgatttat tttacatgta
     4741 aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa taagacgaga
     4801 ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt atccaaacca
     4861 cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca tcacagtcag
     4921 atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt ggacaaagac
     4981 gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct tgtagaggaa
     5041 tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc aaaaatctat
     5101 gctttttcga cgctcgataa aacaacagct actggactgc tattcactag agaacaacag
     5161 ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct agacaagaaa
     5221 caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca gcaggaactc
     5281 caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg gataaacaga
     5341 acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc aatacagttg
     5401 gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca ctatttttct
     5461 agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc aaataacagt
     5521 gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg ggccaacgat
     5581 gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca tataagcaga
     5641 gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca tacatgtcac
     5701 cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc tttctggatt
     5761 tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga catccaaaat
     5821 gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat gaagatcttg
     5881 atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca tcattgttgt
     5941 aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta aatttgtcac
     6001 gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt ttctgccctt
     6061 ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga gaagtaaaca
     6121 aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt gccatatcaa
     6181 taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa agcaaagcaa
     6241 tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc aaagtttcct
     6301 gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac tctgtttgtc
     6361 tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt tgctgatata
     6421 cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg atatgttcta
     6481 ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta cctaaagaaa
     6541 actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg acattttgtc
     6601 ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa cggatttgtt
     6661 tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct aatgttttcg
     6721 cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac aaccgatccg
     6781 gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc gcttgttttt
     6841 tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac tctgcttctc
     6901 gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa ttccccgaaa
     6961 actctcggat ttctccgtct tttacttccc agattttcga tgctagctca tctagaaaat
     7021 agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct tcaatcagtc
     7081 gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc ccttgttcat
     7141 aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc catttccgct
     7201 ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt aggtcctttg
     7261 cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt tggattttcc
     7321 catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta ccgacaccat
     7381 tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca atagatagtt
     7441 ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa ttttccatat
     7501 gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag agcagaaaaa
     7561 tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata actatgacta
     7621 aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa atggacatac
     7681 tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg ttattcaaaa
     7741 gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt attaggttac
     7801 cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct tttattcgaa
     7861 cgtagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc
     7921 tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg cctctgccaa
     7981 ctgatctgct tcgaatccac caattggatt tgtttcatag ccatgatcac gcgcaactaa
     8041 catgaattgc atagccgcta agctagaatc gactttgact acatcgttca tttcttctct
     8101 tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga cttcttttgg
     8161 catcttccct gattcgtaag cttggttata gatttcttcg ccatattcgt aacattgcat
     8221 atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat tgaaacggat
     8281 caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc gccatggttg
     8341 catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca tttcttcgtg
     8401 tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca taattgttga
     8461 aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt atattgcaaa
     8521 gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca tcgctgaaag
     8581 ccggggatag cacggaacct gtatctttat ggtccatttc acaatgctcg attttcttct
     8641 gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt ctctgacgcg
     8701 aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct tgatcaatct
     8761 gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt ctcatcatga
     8821 tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg attttttgat
     8881 cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata atttcctcct
     8941 ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa aagaaaaaag
     9001 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
     9061 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
     9121 ttgattctta tcaaactata gaagatagct cttctgcagt agaaaatttt acaccgccaa
     9181 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
     9241 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
     9301 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
     9361 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
     9421 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
     9481 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
     9541 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
     9601 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
     9661 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
     9721 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
     9781 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
     9841 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
     9901 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
     9961 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
    10021 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
    10081 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
    10141 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
    10201 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
    10261 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
    10321 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
    10381 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
    10441 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
    10501 ttgtattttc tgagacagta gctgactgca aagtactctc tgtatcgttt tcaattcctg
    10561 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
    10621 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
    10681 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
    10741 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttgata aatttgcttt
    10801 ttctacataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
    10861 tcgtttgaat aactatttaa caagtaattc tctttggata ttatccgtta tggattatac
    10921 ttcctgtgtc gtcggacgga taatcatgtt actgacagac atccggtctg gcgtttctat
    10981 cgcaaatgct acagcagaag ctatatcttc ggaggtcaat ccccattctt tttgcgcttc
    11041 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
    11101 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
    11161 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
    11221 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
    11281 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
    11341 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
    11401 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
    11461 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
    11521 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
    11581 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
    11641 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
    11701 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
    11761 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat
    11821 ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt
    11881 tttcttgatg tggattggct ttactgacaa gcctcttcgt tcttttggat cggttagatg
    11941 gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc
    12001 acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg
    12061 tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact
    12121 aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc
    12181 tgctaaaggc tcttcgtttt cagcttggat ctgatggatc acttcaaaga gcggatccgt
    12241 tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc
    12301 ttttatcctt actttataac ggacggctta tcatgtctaa tacttataaa acatacttaa
    12361 ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga
    12421 tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc
    12481 aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga
    12541 acttggtgtg acacttttca ctcggggctc ccgagagata cagttgacgg attctggtcg
    12601 atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt
    12661 aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat
    12721 ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca
    12781 tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt
    12841 tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca
    12901 agatcgttgg ggacttttga tacatcgaaa tcatccttta gcaaagcaaa ccgtcatcca
    12961 tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca
    13021 actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt
    13081 attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg
    13141 gatcatcaat acttatggga cggaaaaaat attcatccca ctcgatccgc cattacaagc
    13201 ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt
    13261 agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt
    13321 aacaacgaat aagataagca aaagatccaa cgattaaata acataagttt cttaatttat
    13381 ttcttccttt tatttctgat aattccagta aattactata attataaaaa aagaattgac
    13441 aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag
    13501 atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc
    13561 tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag
    13621 ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt
    13681 ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg
    13741 atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg
    13801 caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat
    13861 catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac
    13921 cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta
    13981 agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat
    14041 ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat
    14101 attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt
    14161 gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca
    14221 ttttcactga attattcaag actggtgaat catttacgac tgcttctaca acgaacccat
    14281 gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca
    14341 gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg
    14401 atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc
    14461 gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa
    14521 tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga
    14581 tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg
    14641 gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt
    14701 cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa
    14761 atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc
    14821 catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag
    14881 ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca
    14941 agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc
    15001 tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag
    15061 gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc
    15121 atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat
    15181 ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa
    15241 tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa
    15301 acaaagcaaa aacagcttcc ttgatgctag cagcagcttt tgcttcattc tttactggtg
    15361 tgacagaacc acttgaattt tcatttatgt tcgttgcttg gccattatat gtcttgcatg
    15421 caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg
    15481 cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc
    15541 cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact
    15601 ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg
    15661 acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg
    15721 ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt
    15781 tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg
    15841 gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg
    15901 ttgcagatga gatggcaaaa ctacatggtg gtgctgcagc acgacctgca caaacaaaca
    15961 ccgtggttaa aacagaaaca tttgccggtg aaacgatcga acaagatatt tacgctatcg
    16021 caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga
    16081 tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca atcgctggta
    16141 cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg
    16201 aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat
    16261 atgtagaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg
    16321 ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag
    16381 tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg
    16441 tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga
    16501 gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca
    16561 actccgcggt aataagtcca gctaaacgaa acatatgaag agctattttt tatttgactc
    16621 ttcttattac ttgatgcaga acagcttttt cacaaaattt ttcttcatac tcaaaactaa
    16681 tcggcttttt cacaacctct ttatacatta ttcctgatcc aatatttctt ttaatgctgc
    16741 ttcaagagaa gttaatggtt ttcccaatac tttttctaaa tcatcagagg aaatagctaa
    16801 ctgatccttt ttaatatcat gttggatcgc taaaaacatg tcagctgctt cttgagggaa
    16861 tccggcttct tttagatttt cgacaaacgc tgcatcactt gcttcaataa tttctaactc
    16921 tttacctgtc gcacgctcta atgccttggc taattcttca tagtcagtta gattgccgga
    16981 taattctaag atttctggag agtcactttc tactagtact tttgctgcag cttctgcata
    17041 ttcacgtttc aatgcccaac ctgtttttcc atttcctgca gcataaacga attgaccagt
    17101 ttttaaagcc tgcccaatga taggtaattc attctctaca taccaattat tccgtaaaaa
    17161 tgtatgggaa atacctgaat ccttaatcat tttttcagtg aattggtgat ctggcgctaa
    17221 tatactgatc gagtgatcgg catctgcaaa acttgtataa gcgatataag ataccccagc
    17281 tgcttgtgct gccttcacta cattcttatg ttcggcttga cgatttcctg gaacgccaga
    17341 gacaaaaagc aatcgatcta ttcctttcaa cgcttctttc attgagtcta aatcatcata
    17401 atcaccaata cgtatattaa atccagcttc tttcagtgct tctccttttt cttcactacg
    17461 tactagtaca tagatttcat tttgtggaac catctttttt aagaattcca gcgcataacc
    17521 accaaaacct ccagttgctc ctgttactaa ataatgcata tttgccactc cctattcttt
    17581 ttatttgtta agatttacag ctgtacattt ataaattaca cctttatcat aaagagtgtg
    17641 gtaagataag tcaagcaaaa aaaatgaaag gatgacaact gatgaagtat tctctgcaat
    17701 ttagtgatgc aatccatgta cttgcctata ttgaaatgtt tcaagatacc cgatcacttt
    17761 ctagtgagat gattgccagt agtatcgaaa caaatgcagc aaatgtacgt aaaatcatga
    17821 gtcagctcaa aaaatccggt ttgatcttta caaaaaccgg aaaaccgcag ccttcccttg
    17881 ccaaaagtcc agatcagatt tctttactag atgtctatcg cagcatcgaa ggtaatacac
    17941 agcttatcca agtcgatcca aaaacaaatc caaattgcgt cgttggtgcg aatatccaag
    18001 aagctttaga agaaagctac gctcggctgc agaaaaaggc agaagaagaa atggctaaga
    18061 tcacacttga tagtctgata cataaaatag ccgttttgga aaaagaaaaa agaccagaaa
    18121 acacaggcct cgtagagaga tttttataat tctctctact gtgcctgcat ttttctgatc
    18181 ggaatcgtct acataaattc ttttattctc cgggggacaa aagccttgtc atgccccttt
    18241 ttcctctcta atgctcaggt caaagcgctg aagtcatgac gtcttgataa acggtgttca
    18301 agtagctgac cctcggcatt agttctgaaa aatgcaaaat atggaaagct atttcccatt
    18361 tttcctctct aatgctcagg tcaaagcgct actttcacga cctctatatt atcatggttt
    18421 attgactacc ggtatccaga tttctcccat atatttcccg ttggtaactc ccatttcgac
    18481 tacagcattt ggacccccaa cataggcgta atcttctact tctcttaaaa catgcccaaa
    18541 ggccgtattc gtcaaatcat ttcttaattc ttcaggcgat tctgctaaag cgcttacaac
    18601 aagatacgtt ccttttggga atgggatgat tcgcgtagtg tttggaacat cctgactcgt
    18661 ttgaacaccg acatagtaca tcattttccc gttatagggt tcgttgactg taaagaaata
    18721 atgattttca gcagcttgtt tcaatgcatc tattgttcca ttaccgataa ctttttgctc
    18781 aaatgcttgt ttctctgcag ccaaaccgac aaaatccgta ttgctactct tcaattccac
    18841 accaataccc gtcaccgtaa aatctggttt ttcttcgatc ctatactttg ccatttctcc
    18901 caccctttct attatattaa gttcatctta cataaaatta aagggaaatg acacgattac
    18961 gctctttttt ctagggatat ttttattttt taactatttt tttgctttca ctgcaatcca
    19021 aatctcactg taaacattac tgaaatcctc tccccatttt gtaaaagaga tctcgggagc
    19081 ttcgactaac ccataatctg aagaaggaag ccattctgca tagattttcc cccaagtatc
    19141 ttgtaaagtt tggggaaatg gccctttatt aggaaaaatg gcccaagtgt gttcttctat
    19201 agcaagcatt tctaaatcag cgtacggatt ttcttttgtc gtagcaaagc caatcatgtg
    19261 catcaaactg cccttttctt ccaagcggtc tccatcaaat ccataagaaa cgttgattac
    19321 ttgatttgga tataaatctc ctaacttatg catctcttcc ctttgagtct ccgtgatcgt
    19381 ctgcgctaac ttttgtattt cttgattgac tccttcaaat ttgacaggaa cttttttcgc
    19441 cacacctgcc acataaaact gttctttctt ttcgatacga aattccatcg attttcctcc
    19501 tctgacatca ataaaaaatg tcaagcgtgg aaaagatttt tgtatgccgt atctactgat
    19561 ttctgaaggc agatatcctg accaatcacg aaaagctctt gaaaaacctt cgactgtttc
    19621 atatccatat ctaaacgccg tttctgtcac tcttgatcca tctagaagat cgcgattagc
    19681 caaagccaat ctacggtttt tgatatagtc cattagcgac atccctgcaa taaacgtaaa
    19741 gatgcgtttt aattgatatt cagagattcc tgtattttcg gctattattt tataggaaag
    19801 tccttctgtc aaatgttctt cgatgtaatc gatcaattgg ttcagatctt taagcatatt
    19861 tatcttcctc cttgattgcc tttttatcat attatcaaag aaagtcaatt acttgatgaa
    19921 tcaggaacca atttagtcag atgtagaaaa tgttgtatgg atctaaagaa aattctcaag
    19981 cagcgtttcg taacggataa ttggtattat ctgcctatct atgttttacc tttagaaaaa
    20041 taaattttgg ggaagacaga ttctaagtaa acaaagcggt aaaaatatct tttattccct
    20101 tagttaatcc gcttactacc tactgtacaa agcaaattgt tgttcaaact gctgatcaga
    20161 acgctgcttg gatttttctt tgcgatacat catacctctt ttatcaattg gaacaattat
    20221 ttatttctaa caaaatcact tcatttttgt cagataaata aaaaacaaaa aaagttccat
    20281 ctttaaaaca cgctattctt ttactttttc tcacattctc taagaaaaga aaaggagaat
    20341 gaatttttga caattcgagc aaaagtgatt atcaaatatg taaaatcagc tacttttttc
    20401 ttaaacgttt ctttatacta aatataaata agtgtttcat gaaaggagaa ctagatgaat
    20461 aattggcttg agtattttcc tgaaaacgtt ttggaacgcg gttacagcta tcatctgcac
    20521 ggttttgtcc gtcatttgaa ctatacaagc aagtacttgt ccgcaacagt ttctggcacg
    20581 gaagattata aggtagtgat cacttgggat gaaaagacca acatgacttg tgattgtcta
    20641 tatgccatag aaggaaaaaa atgcaaacac atggctgctg tcttattcgc ttacgaagaa
    20701 cgtccaataa aaaaatccaa ctactcactt tccgaactct cgtcccttgt ttcctccgct
    20761 agttcctctc ttgtacggga gcttttaaca gagattctta tagaacatcc gcactttatc
    20821 gaacgcttca aagttaagat gccatttcat gcaataaatt attcagataa attaacaact
    20881 atcatccata aatacgatca tattatcaag aaaaacaaaa acaggaaaac agcgaagttc
    20941 atcatggaga tgcgaaagtt catccaagaa gcagttgaat cgcttattca gcaaaatgcc
    21001 tatcttcccg cattcgaatt gatcaatgaa gtgattgcta ctctcgaaac attctattgg
    21061 gagcctgagg atgaacgaac ccttcttctg atagaggact gttattattt atggaaagaa
    21121 ttattagcag aagcacctca tgcagaaaaa aggcaaatgt tctcttggtt cgtctgccaa
    21181 gtcgatcata ctgacgcctc ttattcaaaa cgctactcta taaaaatcct gaaagaagac
    21241 tttcgggaaa aagaattttc taaccaaaag aaaaaaatag ataaaaaact aaagaaacgg
    21301 taaagaaaga cgatttcaat gaaaaatagc aagtaaacga taacaaccat cctaaatcca
    21361 caagaaatac agcagacctt gctttaaaaa cacgaatcat gcaaaggatg aagtaaagaa
    21421 gacttaacgc gtacccaata ttagtcattt gactattagg tacgcgtttt gttttataag
    21481 tcatgacaaa tttccttcgt tgctggatag attttccggc attgttgata gattttatga
    21541 tacttttcta cattttgtaa gagaggttcc gctgcatctg tatattggat aaaattctga
    21601 caacaacttt caggtgtgtc gaaccaccct aaccctagtg cagcaatcat tgttgcgcct
    21661 aatgcaggtc cttcctccac acttaaacaa tagaccgttg catcgaaaat atcagcttgg
    21721 atttgtagcc atgcaagatt tttcgcgcct cctcccaccg aaacaatacg tgtaaatctg
    21781 cgacccgcta gttctttacg tttcaatccc accattgaca aatgctcatt aaaaatttag
    21841 aatattcaca agtttaacga caattatgaa aatttatgtc gtgctaaagg agttttgtta
    21901 ttggatgaaa agttagatat aaaaactgcg atcaaagaca cgttacctac cgttttcggt
    21961 tatatcggta ttggacttgc atttggtatc gttgggaaag ctgccggatt tcatccacta
    22021 gtcgtcacgt tgatgtccct gctggtctat gctggttctg cccaatttat cacagtcagc
    22081 atgcttgcta gtcacagccc attgctttcc atcgttttct cgacctttct agtcaattcc
    22141 cgaatgattc tgatgagtat gacgattgct ccttatttca agaaaaatcg gctgcttcaa
    22201 aatctgctga ttggcacatt gctgacagat gaaagtttcg cactgggaat gaataaattg
    22261 aactatactg gacaaaagtt aaactttcgt tggatgaata cagctaattg gatttcttat
    22321 ttgacgtggg tgggttcttc tcttgttggc gcacttttag ggaattttat tactgatccg
    22381 aaaaagtttg gtttggattt cgccatcgtc gctatgttta tcgggttact ttatcttcaa
    22441 gtcatttctg atagaaacac ctctaaacga ttacagttga tcctgattgg tctcactttg
    22501 attctggtat atgtcggact gatttttatc ccaagcaact tagtgattgt agttgtaaca
    22561 ctgattgggt gcggattggg ggtgtggatc aaacatgcct tcttttgagt ttacattgct
    22621 gactatttta ggttgtactt tagcaacttg gctttctagg gtcctaccat ttgtcttact
    22681 gaaaaaattc gatttaccgc aaccgttgct tgagtattta agttttgtcc cgatcgtcat
    22741 tatgtctgct ttatggttca gcagtctatt tacccaaaat atcggacatc tgccgcagat
    22801 caatctcgaa aatgctttgg cttcagttcc tacactttta gctgctattc tttcaaaaag
    22861 cttattggtg atcgtcttgg caggaattct ttccctttcg ttgattcgct tattataaat
    22921 aaaaaaacaa gagcttatgg caaattactg tcatgcgctc ttgtttttat caaagtcaaa
    22981 taagaaaaaa atcggcgtat tcttgcttat ccgttgaacg gaccactaac aacgactttt
    23041 cctaacatat gtcctgtttc tacgtctttg gttgcttgtt tcaagttctt cgcattcatc
    23101 ccatctgaat agaccttgct caatgtactg cggataatcc cttcatttgc taaagcagca
    23161 atgtgcgcta aagcttctcc ttgcgtttcg atttcatagt tatagtctgt tttagcgaac
    23221 atatactccc agtcgaagct gatagattgg tttttccaaa cacctatatc taacggctct
    23281 ttgatcccta cgatcgttcc cacatgtcca aatggtcgga caaggtgctt gatttgatca
    23341 aagtagcggg taatatcgaa taatacagcg acatactcta cttgatcgat tcctaattca
    23401 cgaaaacttt tacttaagtc gtgatgataa tcgattggat agtctacacc tgtttttttc
    23461 agccattcaa aattcttcgg acttgcagtc gcgtagactt ctaggccagc ccaatgcgct
    23521 agttgattca aaatcgaacc gacgcccccg gatccgttga tcactaagat ttttttcccg
    23581 cgattggcat tttcttctgg agttagacca aatttttcaa ataacaattc ataagcggtc
    23641 aatgaggtca acggcaaggc tgcagcttct tcatctgaaa gattttttgg tgcaagagcg
    23701 acaatacgtt cgtcaactag ttggtattct tgattgcttc ctgctcttgt tgttgtacct
    23761 gcgtaaaaaa cgcgatcgcc gacagaaaat ttttgcactt cgtcacctac agcaacaact
    23821 ttcccgactc catcaaaacc taagatacgt aaagcatttc ttatcccgtt atcttggcga
    23881 agtttcgtat cgacaggatt aacagatact gcgttgactt ttaccagaag atcccgtgaa
    23941 ctgggtacag gtatctgttc tttttcatca ataaagcttt ttggatcatc gatcggcaac
    24001 ccttcataaa aaccgactac cttcattaat tccttttctg acatacgaat tcctccttag
    24061 cttcatcaag ttggagcatc catacagtgt tcatttcaat atatatagta tgctatttgt
    24121 tccacctttt ttattcatca taattattct cttacttaaa gtaaactatt tgcacttttt
    24181 tggttaaaaa agaggttgcg acaagacttt tgtcacaacc tcttataaac ggtgttctcc
    24241 taccaactct tcggtcacaa gtcaaaatat tcatcaaaca tgagaagctg ttttttgaaa
    24301 ttgctccttg tgactcagag ttaaacggca ggctcacaac ctctgatata aacggtgttc
    24361 aatcaactga ccctcggtat tagctcaaaa aacgacaaaa tatgaagagc tatttttgtt
    24421 tggctgttct tattacttga tacaggacag ctttttcacg acctctaatt cacggtgttc
    24481 aagcaagaaa tcattgttca ctgaaaagcg ttggatgttc tttttcaaag gtctctaaga
    24541 tctctcgtac ttcatcagta gatttcgtat tcatcaactg aactcgcaag tcatttgctc
    24601 ctggaaatcc tttgatatag attttaaaga aacgatgtag tcctgtgatg gaacgcggga
    24661 tgatttctga ataatgatct tgcagatcta gttgcagacg aagtagtccg atcagttctt
    24721 tttctgtatg ttccctcggg tctttttcaa atgcataagg attcttgaaa atccctcggc
    24781 caatcatgat gccatcaacg ccatatttct cggccaattc ttctcctgtc tggcgatcgg
    24841 ggatatctcc attgatcgtg attagtgttt gtggagcgat ttggtcacgt aaagcaatga
    24901 ttttaggaat gacttcccaa tgtgcatcta ctttactcat ctctttacgc gtacgcaaat
    24961 ggacagaaag attggctata tcttgatgaa gtaggtgact gatccattct tccatctcag
    25021 acatttccgt atagccgata cgcgtcttga cgctgacagg caaaccgcca gctttagctg
    25081 cttcaatcaa ttgtgccgct acttctggac gaaggatcaa gccactgcct tttccacgtt
    25141 ctgctacatt agggactggg cagcccatgt tgatatcgat tccttggaag cccatttctg
    25201 ccatagcaat gctcatttct ctaaaaaatt ctggcttgtc tccccaaata tgggcaacca
    25261 tcggctgttc atcttctgta aatgttaatc ttcctcgtac actgtctttt ccatctggat
    25321 gacagaaact gtctgagttt gtaaactcag taaagaagac atccggtgct gctgcatgct
    25381 ttacgacatg gcgaaataca acatctgtta catcttccat tggtgctaaa acaaaaaaag
    25441 gctttggcag ctctttccaa aaattagtag tcatttattt gttactctcc attctatcaa
    25501 gcagtgaagt cgcaatcttt ctcaaaaaca ttgtcattat actaggaaac aaaaaaagaa
    25561 gcaaagtgaa cagcttaaaa gatgtttctg attgagcaaa aaaaagactg tgacaaaaat
    25621 cttatcacag tccattttcc ggaataaatg gtgttcaaaa gcttgcattt gcggtaataa
    25681 gtccaaccaa acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg
    25741 cagaacagct ttttcacaac ctctccacta aacggtgttc aatcagctga ccctcggtat
    25801 tcgctcaaaa aacaacaaaa tatgaggaac tattttcccg tttgtttctc taatactcag
    25861 gtcaaaacgc tgatttcaca acctcttttc taacttctca atcctcttcc tttattgatc
    25921 aaataccaag taacggcgta caaaacaacg atgaataaca caagtacgat catcgatgtt
    25981 gctactggca catcgatcgt tccaaggaat ccgtaacgga aaccggagat catataaacg
    26041 atcggattga cttttgaaac agcttgccaa aagggcggta acatagagat ggcatagaaa
    26101 acgccaccta gataagttaa cggctgcaag acaaatgtag gcacgatcga tacatcatca
    26161 taagactgag caaaaatccc gttgagcagt cctgctaatg aaaacaagat tgccgtcatc
    26221 aataatgtaa tgatgaccat gaaccatgaa tacacatgaa gtggtacaaa gaacaaggaa
    26281 atgatcgtta cgagagagcc gacgagaata ctgcgaccca caccaccaat cacaaagccc
    26341 caaataatca catgcgtagg aacaggtgct accaagattt cttcgatatt tttctgaaat
    26401 ttttgagaga agaaggacga agaaacattg gcatacgaac tagtgatagc agacatcata
    26461 atcaaaccag gaacgatgaa ttccatatat gaaaaaccac ccatgtcacc gatccgccca
    26521 ccgatcattt ttccgaaaat aacaaaatac aaggatgtcg tgatcactgg cggaaccagt
    26581 gtttggaccc atatacgcaa atagcggttc gtttccttga tagctaaact ttttaatgca
    26641 gtaaaataaa gattaaacat ttttctcctc cgtatgatta ttttcttctg tgattttcaa
    26701 gaacagttct tctaaacgat tcgacttatt cctcatagaa agaactttga ttccttgtct
    26761 tgtaagctga tcgaagattc cgttcacccc ttgatttcgt tcaacttcga cagcgattgt
    26821 ctgctcatct tcaaatgtac aagcatatcc ttcaatcaca ggtttggtat catatggcgc
    26881 tagatcaaag ataaatgttt caaattgcag ttttgacaga agctctttca tacttgtatt
    26941 ctcgatcagt tcacccgatt ggatgattcc gatgttgcga caaagcattt cagcttcttc
    27001 tagataatgc gtcgtcaaaa tgatcgttgt accattttcg ttcaattctc gtagaaactc
    27061 ccacatctcg cgtctcaact cgatgtcaac tcctgctgtt ggttcatcca agatcagcaa
    27121 acgtggttca tgcatcaatg ccctagcaat catcagtctt cgcttcattc ccccggaaag
    27181 catacgtgca cggacatttc tcttttccca aagattggac tgcttcaaat atttttcgct
    27241 gcgtttcatc gcttctttgc gagagacacc gtagtagcct gcttgattca cgacgatttg
    27301 ctggaccgtt tcaaaagggt taaaattgaa ctcttgtgga accaatccaa tctgttgctt
    27361 tgctcgaact agatccttgt ccagatcata accgaagaca ttcacttttc cagatgtttt
    27421 attgactaaa gaagtaataa tcccgatcgt tgtagacttc ccagctccat ttggccctaa
    27481 tagcgcatag aaatctcctt ctttcaccga aagatcgatt ccccgcaatg cttcgacccc
    27541 acttccatag atctttttca aattatttat ttcaagcgca tttgccattt atttcctatc
    27601 tccttataga catgattctt aattgtatca tggaatacag cagatgaaaa aacaacttgg
    27661 cttccacaca aaaacacacc tgctaacaaa gaggagatgt gttttctagt tcatgatcat
    27721 cttcgataga ataactatct aaacaactct ggaactttta aaatcataaa agtaccgaca
    27781 gcaactacta cgatgacata tagaagatcc ttataactga ggttcttgtc ttttttattc
    27841 atgttaagct ttcctccgtc cttttatcga aagctttttt cttttatttt aaccgaaata
    27901 aataaaatgg aaatagttat tttcaatttc tgttttcgct gtttgtttta cggagaactt
    27961 tgccatggaa cgtgcgtctc gtcgctgatg ctaagttttt tcgataaaag ctgctagaag
    28021 gaagacagcg tggtttattt tgtatcgtct tttgctaaat cttccaagat catctggaca
    28081 gcagactcgt tcccattgaa ttctgccact tttttggcat caatgtactt cttttgataa
    28141 tcgtcttttt ggacccacgc atcatgcttc gtttgataaa gttaatcaaa cggataccac
    28201 tgacgctgtt catcgaacaa caaactttct ttttgggcct gttcaactat ggatttagga
    28261 taattcaaaa ctttcagcaa ggcaatcgca tttcgggtcc ttgaaggtcc ttcttttaat
    28321 ttgaaatcaa aactgattcc ttttccttct gttacttgtt cttcaaagtg gacattctca
    28381 caataattct ttaaaatctc tgtcaattcg atatcatgtg tggcgacaaa tgctaaagat
    28441 ggatactccg caagccaccg aacgactgaa gcggaagaag caatccgttc gatcgtattt
    28501 gttcctttta aaatctcatc tacgaagcag tagcagcgtt ctttggaagc cacttggtcc
    28561 agcaaacgtt tgatcgattt gatttctgaa acaaaataac tgtcgccttc aaataaatca
    28621 tcttcgattg ccatggaagt caacacatgt ccaggttgca gggtaaactg ttctgcaatc
    28681 gctgtttgga tcgtctgtgc tagaatacag ctgatggcta tacttttgac atacgtagat
    28741 tttcctgatg cattggaacc cgtgactaat gtattcttct gccagttgac tggattaact
    28801 accgcttctt ttaacaatgg atgatagata tctttcgctg tcacaccgcc ctcagtaaac
    28861 tctggtcggc aagtgatcgg catataggtg cgataattca aaacggctgc tgcaacttcc
    28921 aattctccca gcaattccca cagcttgatg gcttcagttg aatgcttttc taatttagtg
    28981 agaacgaaat gataggagat aaatggcagc atgaacatga tgttgacata ttcaaaaatc
    29041 aattctgctt cactcccgct cttcattcga aatgaaaaac ctaatttagg gatgtctctt
    29101 aatggtttag tttgctgttt cagctctttt tgaagtggtg tcgtcatttt tgctaactga
    29161 ttggcggctg aaatcgttga aaccaaataa ctcatgctat tcagctctgt ttctagtgtt
    29221 tgtttcttca ctagataata gaccgtattg aatactaagc caccgattgc tagatagatt
    29281 cctagtactt gccctaaaag aagcaaacct acacccacca gcggaagcaa gccaagaaaa
    29341 ataaataaac ctacgcgtcc aatcttttta ctttttccat ccgctagata ttgttttgca
    29401 aaattatgat ctttttttcc taaacgagcg aattgatatt ggattctttc tctcagttga
    29461 ggattttcct gataaaaagc aattagtttt tctaactgat aatcttctcc aaaatcaaag
    29521 ctacgtaaac gctgatacaa cgcttctgaa cctacactag aataggtact gttgatccct
    29581 tcaaatactt cgaacatatc caagtcatac caagtaagat cgtcgatttc actatcccat
    29641 gaacggaatt ttttctccgt gagccacgca tccttcaaac tttcttcttt atcaaatctc
    29701 ggttgataag gaatttttcc ccaattacta cgaactgttt cctttacttt caaacgtgaa
    29761 aaaatgtcta aaacaactaa taaaataaca actgcaataa taattaaaac gataaattct
    29821 ctttggccca tatccctctc cgttccttga tttgttagtt ttagtatagc actgttgcgc
    29881 tatttgaaaa gggggtacaa aaataactgt cttcgttacc taattcgtat caatcacacc
    29941 aaaataaaaa caccttccaa aaaagaagat gtctttaaaa cgagatacaa ttttttgtca
    30001 ttcacttcag gtaaattaga gccaattaaa ccctccgcaa cttgacggag atggagaaga
    30061 tgcccccgtc taatctagcg gtcatctctc ctcccattcc ttccaccaat ttttttgaca
    30121 ggtaaagccc gattccgcta gattccgtat ttgtgcggct ggtattttcg gtataaaaac
    30181 gctggaagac tttttcaaca gaggtttgcg ctcctggttt gataggattg gccactgtta
    30241 atcgaatgaa tgaatcctct tttgaaaatg tcacctttgc ttgtccatct gcataacgca
    30301 ataaattctg tatgatattt tgcagcactc gcagcaacat gagcggatct gctaatacag
    30361 ttgtatttat cgagtgttca gcgaacgcta cctcgatatt tttctcagaa aattgttcat
    30421 aaaaagagaa aaatacttct tccacctctt tagaaaaaga aagcgtatgg agatccattt
    30481 ctttttgctt cgtctctatt cgagcaagat cataaaactg atccgtcata ctgagtaatc
    30541 ttgcaacaga ttgtttgatg atcgttaaat acttctcccg tttctccgta tcctctacct
    30601 gttccagtaa ttgtacataa ccgttaatag cagtcaaagg agttctgaga tcatgagaaa
    30661 ggccgacgat cgatagtctg atgttttctt ccatttcttg aaatgcttgt cctttttgtt
    30721 cattttcatc cacccatgta ttcagtgcat ctactaggtc cattagattc ttttctcgaa
    30781 attcgactga aagacgacca ccatattctt gtcgttcaga aagagtggcg attttcttag
    30841 ttaatctctt gatttccctt tgcaggaaga aaataaaact tcccataaag ataacaacga
    30901 gaagcaacag ccacttaatc aaatctaaac cccactcccc acaccgtatg aatatacaca
    30961 gcatgtgcct ttgctagttt ccctcgaaga ttggaaatat gcatattcac tgttttctca
    31021 ctatcaaaat atggttcttg ccaaactgtt tcataaagat ttgctcgcga aaatactttt
    31081 ttcggatgtt ctaaaaaaag cttcagcaga tcgaattctc ttcctgtcaa atggacagcc
    31141 acaccattca catttacatc cctcgactct ttgtccagta agatttcctt gtaatagatg
    31201 cgttgcgaca gatcttttac ttggacagaa gtctgatgac gaagttggat gccgattcgt
    31261 aacaacagtt cttcgatatc aaatggtttc gtcacatagt catccgctcc tgctgccaac
    31321 aaggaaactt tatctctttg atcgcttttt gctgaaagaa cgatgatcgg aacattcgat
    31381 gtctgtcgga tttctgctaa caatgcttca cctgaaagcc ctggaagcat gagatctaga
    31441 aggatcaaat cgacaggata tgtgcttaac aataattttc cttccgtacc tgaataagca
    31501 tcaagaagtt tgtatctctg ttcaagaacc tcctccagta aagaatgaac agcttcatcg
    31561 tcttcgataa tcaaaatagt ttccagcagg aacacctact taatgtcaga tttttggaaa
    31621 ataaacattc ctagagctga tgataccacg attgttacta gactgaccag aatcattttc
    31681 ggtgcttgcg aataagttgc ttgttgcgca aagtccgtat acatgagcca cgtttccgta
    31741 aaccactccg ttagaaattc caagcctttg atttttgcga aaacaccggg aacgaaggcc
    31801 gttcccatga tataaagaaa agaaagaagc aaactgacac tggtacttct agtgatgaat
    31861 gtcaaagcca ctggaacaga taatgtcgct aatatcagca gaagcaatac aggaaatact
    31921 tttagaacat aatttccata gtttttgatt ccctcaactt caccaaatcc gaaagcaagt
    31981 gtgtaagcaa tcatggctag ccccgtaatc aaaaccacgc caagtgttgt gatcaaaagc
    32041 gtaacgagaa gtttcgacac ataataagtc attcgattcc gtcctaaaga taacgtattg
    32101 cgaatcgtac cgttttgaaa ttcttcccca atataaaagc acgcaaatgc tgcgaaagcc
    32161 agaagaatcc atgcagccat tttttccacc attaaaatcc ctgttgcacc gttgatgact
    32221 gtatccgttg cttggtttaa ttcattgccg tattttgtat gcatatacca ttcgttccaa
    32281 gaataaaaaa gcggaagtac gaccatcagt cctagtgaga cccacatttt acggctcttc
    32341 catactttta ttttttcagc agttaataga ttaaacatgt ggattgcctc cagttaattg
    32401 taagaaataa tcttccagtt tttgcccaac taggtggata ccatccacag gaacttttgc
    32461 ctctaccaat gcttgattga tggctgctac ttggtcaatt ccttcaaaaa gctggataac
    32521 acggctagat tggacaaaat aatccctata accaagagag tcgagaaccg ttactgcttg
    32581 ttccgtcgcc gaagtgtcaa tttttataaa ttgacgagat tcttgtgcca attcttcttt
    32641 cgataattct ttgatcaagt gtccttcatg caaaataccg tagtgagtgg cgatctgtga
    32701 caattcatct agcaagtggc tagaaagcaa gacggtgatg ccccgttctt ttaccaaacg
    32761 ttggatgatt tctcgcattt ctgccatccc tgctggatcc atcccattcg tcggttcatc
    32821 caaaatcaaa aattctggat tcgtgatcag tgcatttgca atagctaaac gctggcgcat
    32881 acctaaagag aaattctttg ctttcttttt ccctgtattt tccaatcgca tcattttcag
    32941 caggtcttcg atttctctat ccgacacacc gccgttcgct gcttggatac gtaggttatc
    33001 tcttgcagtt aattctggat aaagagcagg ggtttcaatc gattgaccca tacgtcgtct
    33061 tgctgcctgc aaatccgtcg ttccaaataa ttcgatcgaa ccttcatcaa tcgagatcaa
    33121 acccataata gttctcataa aggtagactt acccgctcca ttctccccaa tcaaaccata
    33181 gatcattcca cgtttgattt cgatcgatac tttatctaat gctttggcag caccgtattt
    33241 tttggttata ccagttgctt tcaagaccgt ttcatttcca aaatccatac ttatccttct
    33301 ttcttattga taagtgaagt atagttgacg ggtataaaga aagcagccag aaatgtataa
    33361 agaaagtgta aattttccaa ttgaatttca agaaaaatca gactgtctcg cagacgtggt
    33421 tgagttgtct gccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc
    33481 atggtaatcg ttgttatcgt tgacatcaga cgtccttgta tatgcgttgg aacttctcgc
    33541 tgaagccaga ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa
    33601 aaataataca tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag
    33661 acttttcaca atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa
    33721 gtcacaatat ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag
    33781 ctgaccactt ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat
    33841 ctttttcccg tttgtttata gatcgaatga tattatagac caagaatcct acctattaac
    33901 atagctaaaa acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga
    33961 ttaaggtaac tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg
    34021 aacgataatc gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct
    34081 ggttttttct tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg
    34141 taaatatatt ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta
    34201 atttaaaggc agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat
    34261 ccattctatt cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga
    34321 gcatcttatt tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa
    34381 aagaaattat gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt
    34441 gctcctacga ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta
    34501 gatttagcac atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa
    34561 gtcactgttc cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa
    34621 ccaggagtcc cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat
    34681 atgtccgata aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat
    34741 actcatattg tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca
    34801 ataaaacgat cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa
    34861 gccattgacg attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta
    34921 gggcaatcga ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt
    34981 gtggaaaatg ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca
    35041 aatgccaatt caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca
    35101 cctgtcgcct ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct
    35161 aatacctaca caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc
    35221 gataaagaat cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact
    35281 ttacctgcaa aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct
    35341 catggtacaa aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc
    35401 acccccatga ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca
    35461 cgggaaaaag ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg
    35521 aatgacaatt attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt
    35581 gaaatcgttc aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt
    35641 gctaatgcgt tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc
    35701 gtgaatacgt tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagccctt
    35761 gttaccagta acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa
    35821 gaagacaaag atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac
    35881 accttgttta ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag
    35941 ttagcgaagg gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac
    36001 caagttgccg ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc
    36061 gaaggatata gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc
    36121 gaaaaaaatg ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc
    36181 tttgatttct ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat
    36241 ctgaccttta aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa
    36301 gcgaccacag cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt
    36361 ccttatgggg attatttact tgaagaagta gaagccccag aaggttttca aaagattacg
    36421 ccattagaaa tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat
    36481 gtctttacca ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac
    36541 gagaagctga cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg
    36601 cctgaggaag aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta
    36661 acttctctgg attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa
    36721 gaagactggt atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa
    36781 aaagatgaaa aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa
    36841 gagaaaacag gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa
    36901 actattgtct tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag
    36961 ccagtagcga aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa
    37021 caccatgttt ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat
    37081 ggtgatgtgg tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca
    37141 aaagaagctt tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt
    37201 tggaaatctg gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt
    37261 gcgaaaaaag tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc
    37321 aattacgata aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct
    37381 caaaccttaa caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca
    37441 ccaactgttc caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc
    37501 ggggagaaaa attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg
    37561 ggctattatt tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag
    37621 aaccaacgtg cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta
    37681 acaatgaaaa gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac
    37741 ctttggaaag ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa
    37801 tactacggtg attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat
    37861 tgaggtggta gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa
    37921 agccgtgaat ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga
    37981 ttacgtgttg tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac
    38041 agaaggtatt gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg
    38101 ccgagaagta cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac
    38161 ctataattta aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt
    38221 acccttacgc gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac
    38281 cgttgccaat tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga
    38341 tttagtcttg aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa
    38401 ataggtgaag attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg
    38461 aaaaggcgag ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt
    38521 tctgattata catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt
    38581 actagactgg ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt
    38641 atgtcggcag gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga
    38701 aggcttttta aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac
    38761 tagcataact ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg
    38821 aaaccatcaa aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc
    38881 actttgttct tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc
    38941 aagagtttag acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt
    39001 ctataaaaga tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc
    39061 tttatattca cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc
    39121 ggatgattct agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca
    39181 aagatttgaa tagtagccga accagagaga ctatcagtta cttccccaaa atctattatc
    39241 gaatgaaaga tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc
    39301 agcttttaaa attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg
    39361 aactcaaaga ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg
    39421 ggatagacga agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg
    39481 cttatgattc tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt
    39541 tcctcctcac cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc
    39601 ccaaaaatgc agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg
    39661 aagaaatttc tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa
    39721 tgaaagaaat gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca
    39781 actttttaat ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg
    39841 cggtgatttt gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc
    39901 tgattttggc ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatca
//
LOCUS       DAFBQH010000018        39136 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.018, whole genome shotgun sequence.
ACCESSION   DAFBQH010000018 DAFBQH010000000
VERSION     DAFBQH010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.018"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..320)
                     /locus_tag="LK694_001004"
     CDS             complement(<1..320)
                     /locus_tag="LK694_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051891.1"
                     /translation="MFKVYFPFQWLFKGILLLLLGLILFGSYLTIKARYFPEEKPVKI
                     VTKDLKMGETYQADGVEITFKKVELTEKRNEFAQHDRVLQVTYTMKNDTENPIRPQQW
                     SIEN"
     gene            complement(313..615)
                     /locus_tag="LK694_001005"
     CDS             complement(313..615)
                     /locus_tag="LK694_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051892.1"
                     /translation="MSDPYIKVYCSKETKTIFDSFYEQNKGIFGTKANMFRVMVSNLP
                     VLANQSNVKLSNPESKGIEQKISEIESIVVGKLDEIDQKLSYYLKNKCKTEEGKDV"
     gene            complement(831..2222)
                     /locus_tag="LK694_001006"
     CDS             complement(831..2222)
                     /locus_tag="LK694_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBK5051893.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDNSEE
                     ALQEMNLVRTRMNKSEDIAQAFHVIHSFDKNISKELSIEKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNIDMETCKRLRIDPKDLERMRKINDEVLVENGLEPVDNQY
                     YENLEKRTNLYSNDPSKLKNGKINYQEFVQNAIEATLENCEVNSFVRFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQDVNFYERRDELITLGREKQSRKEKSQYVERTFSARKLGK
                     KYSFESVFERLEKNLEETHMNNFVDLDTEQIIALASGVDYKTGELNVPFVELDSIAQK
                     QFSDHEGAKKIIKYDDFLREVDRHYANTISLEDDQEYTIKLHTDVNPRTLRSTKSKTI
                     LVKKTDQKELFLPAEGFKNCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQRRQRDFEMDF"
     gene            complement(2207..2818)
                     /locus_tag="LK694_001007"
     CDS             complement(2207..2818)
                     /locus_tag="LK694_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBK5051894.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVALIKQLNQIGNNLNQAVKALNIIAKYFREQSENKKEK
                     SLHFSFDVSRYNIFKDELANSIAKKMMISYPDDIFLESNRSTSSFLKLSEVEKDYFQY
                     WLEKQTRPDTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            complement(2830..3018)
                     /locus_tag="LK694_001008"
     CDS             complement(2830..3018)
                     /locus_tag="LK694_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051895.1"
                     /translation="MQLILQISTQDFLELAELCDSVKKATNEKITVTNYIELMIQEEL
                     MMKNEIESNFKCCSLEKM"
     gene            complement(3032..5677)
                     /locus_tag="LK694_001009"
     CDS             complement(3032..5677)
                     /locus_tag="LK694_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051896.1"
                     /translation="MKIIKKKIANRKTNWLPVIFLVLIAVILFTSNIAEGSIFNWFDD
                     TESQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAVVDGIQNMIPEVLDLF
                     SFVESTGLNKVYQAVLSTIVVGLMILSLVIIGYKMITGKGTVDLKSVGMNVVMSISLI
                     LLMPTLIHSGIEFSKTFYNDTTTITNSDEGVAWSLIEQGVTDLAYINKTDQYSMIDKT
                     NDRNNLTKENFWKTDLTQVLTDNVLDKMEKENPIADNLRYELVENSDNEFVATKFSDN
                     FLSTFVEGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRVLGVLV
                     FATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFVNYLGTGSGSSVNPFIKAVA
                     YVCAVIVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRSKGGKN
                     SGGNDQAEGEERKPERNFGQSLSQKANKTGKTLGYARERGLSGLASDGATMASERATK
                     PFKSMRDMANDTKNKFKEGLDDGTVYAINKNSKPIVAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNINPASAIQQKVKQDIEERKNAMHGQAKS
                     AEVLINQKRQEAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGL
                     EKSVKENLQNSTSSQDRRSVDVRENLQNTTSSQNGNRTVYVRENVQSSGTSQPRTVDV
                     RENLQNTTSSQNGNRTVDVRENVQSSGTSQPRTVDVRENLQNTTSSQNGNRTVDVREN
                     VQSSASNQPRTVEVRETRAEASQGDKTQKINIIEERKERPNEKVIVEQEVKEKKRPRF
                     TYENNPLFQDISDKPNPLFDSLFKK"
     gene            complement(5664..5882)
                     /locus_tag="LK694_001010"
     CDS             complement(5664..5882)
                     /locus_tag="LK694_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051897.1"
                     /translation="MIENHLVDKIHCLDQNADTLELTESICSNTIVLDLKAVNDDYVT
                     HIIIDRKIAQKLSESLRKWAEGGNNENN"
     gene            complement(5884..6927)
                     /locus_tag="LK694_001011"
     CDS             complement(5884..6927)
                     /locus_tag="LK694_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBK5051898.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKNKMQMFPNFYYLLKPVLCKDDVYKINKITHQENSQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLFESLS
                     TKNNKLDEELIETFIEENLLKQMWGESIVNCLKLASNSDYRQFDNWYKKFNYAIKSAE
                     KEQKVQLKIIYEICNNSYFVDHVREQLAMTLRDLIRRAKTDHRIKQKNNYIFTSLKNK
                     ALELIKLQKKEGI"
     gene            complement(7082..7408)
                     /locus_tag="LK694_001012"
     CDS             complement(7082..7408)
                     /locus_tag="LK694_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051899.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(7416..7619)
                     /locus_tag="LK694_001013"
     CDS             complement(7416..7619)
                     /locus_tag="LK694_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5051900.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKSK"
     gene            7694..8344
                     /locus_tag="LK694_001014"
     CDS             7694..8344
                     /locus_tag="LK694_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5051901.1"
                     /translation="MSIKTLLNLWFLCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            8399..9565
                     /locus_tag="LK694_001015"
     CDS             8399..9565
                     /locus_tag="LK694_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK5051902.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(9654..10046)
                     /gene="rpsI"
                     /locus_tag="LK694_001016"
     CDS             complement(9654..10046)
                     /gene="rpsI"
                     /locus_tag="LK694_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBK5051903.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(10062..10505)
                     /gene="rplM"
                     /locus_tag="LK694_001017"
     CDS             complement(10062..10505)
                     /gene="rplM"
                     /locus_tag="LK694_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBK5051904.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(11024..11491)
                     /locus_tag="LK694_001018"
     CDS             complement(11024..11491)
                     /locus_tag="LK694_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBK5051905.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(11611..13149)
                     /locus_tag="LK694_001019"
     CDS             complement(11611..13149)
                     /locus_tag="LK694_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBK5051906.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            13229..13915
                     /locus_tag="LK694_001020"
     CDS             13229..13915
                     /locus_tag="LK694_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBK5051907.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(14116..14643)
                     /gene="msrA"
                     /locus_tag="LK694_001021"
     CDS             complement(14116..14643)
                     /gene="msrA"
                     /locus_tag="LK694_001021"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBK5051908.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14727..15404)
                     /gene="pgmB"
                     /locus_tag="LK694_001022"
     CDS             complement(14727..15404)
                     /gene="pgmB"
                     /locus_tag="LK694_001022"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBK5051909.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(15401..16531)
                     /locus_tag="LK694_001023"
     CDS             complement(15401..16531)
                     /locus_tag="LK694_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBK5051910.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(16531..18714)
                     /locus_tag="LK694_001024"
     CDS             complement(16531..18714)
                     /locus_tag="LK694_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBK5051911.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(18711..19736)
                     /locus_tag="LK694_001025"
     CDS             complement(18711..19736)
                     /locus_tag="LK694_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK5051912.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(19781..20608)
                     /locus_tag="LK694_001026"
     CDS             complement(19781..20608)
                     /locus_tag="LK694_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBK5051913.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(20605..21672)
                     /locus_tag="LK694_001027"
     CDS             complement(20605..21672)
                     /locus_tag="LK694_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBK5051914.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(21698..22534)
                     /locus_tag="LK694_001028"
     CDS             complement(21698..22534)
                     /locus_tag="LK694_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5051915.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(22531..23400)
                     /locus_tag="LK694_001029"
     CDS             complement(22531..23400)
                     /locus_tag="LK694_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5051916.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(23470..24753)
                     /locus_tag="LK694_001030"
     CDS             complement(23470..24753)
                     /locus_tag="LK694_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBK5051917.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(24767..26452)
                     /locus_tag="LK694_001031"
     CDS             complement(24767..26452)
                     /locus_tag="LK694_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBK5051918.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            26727..27752
                     /locus_tag="LK694_001032"
     CDS             26727..27752
                     /locus_tag="LK694_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5051919.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(28347..32021)
                     /gene="rpoC"
                     /locus_tag="LK694_001033"
     CDS             complement(28347..32021)
                     /gene="rpoC"
                     /locus_tag="LK694_001033"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBK5051920.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKSVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(32079..35696)
                     /gene="rpoB"
                     /locus_tag="LK694_001034"
     CDS             complement(32079..35696)
                     /gene="rpoB"
                     /locus_tag="LK694_001034"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBK5051921.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            36424..37392
                     /locus_tag="LK694_001035"
     CDS             36424..37392
                     /locus_tag="LK694_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK5051922.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            37410..38315
                     /locus_tag="LK694_001036"
     CDS             37410..38315
                     /locus_tag="LK694_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5051923.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            38312..39118
                     /locus_tag="LK694_001037"
     CDS             38312..39118
                     /locus_tag="LK694_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5051924.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
BASE COUNT        11036 a         8216 c         6922 g        12962 t
ORIGIN      
        1 ttattttcaa tcgaccattg ttgcggacgt atcggatttt ctgtatcatt tttcattgta
       61 taggtcactt gtaatacacg atcatgttgc gcaaattcat ttcttttttc ggtcaattca
      121 acctttttaa acgttatttc gaccccatct gcctgataag tttcacccat ttttaaatct
      181 ttagttacaa tttttactgg tttttcttct ggaaaatatc tggctttaat tgttagatag
      241 cttccaaaaa gaatcaatcc tagtagcaat aaaagaattc ctttaaagag ccactgaaac
      301 ggaaaataca ctttaaacat ctttcccctc ctccgttttg catttgtttt ttagataata
      361 cgataacttt tgatcaattt catccaactt tcccaccaca attgattcta tttcagaaat
      421 tttttgttca attcctttgc tttcaggatt acttaatttt acatttgatt ggttagctaa
      481 cactggtaaa tttgatacca tcacacgaaa catattggct tttgttccga aaatcccttt
      541 gttctgttcg taaaaactgt caaaaattgt tttagtttct tttgaacaat acactttaat
      601 atacggatca gacatgtgtt tttactcctt atcttttttt atttagggag tctgtaggga
      661 gcttgctccc tcagaggttt tccccatcta acttgccaga aagtagccct gcgtaagcaa
      721 gggcggattg actggcaagt agatgggtag ctggcgtatg ccctcaaaac cgcctctttc
      781 caattccctc cgtatcgatg ggaaactgga aagtctactg gctaattcct ttagaaatcc
      841 atctcaaaat ctcgttgtct cctttgctga ttttcaatca tttgttgcgt gaactcgtaa
      901 tcttttttag cacgatacat tttcacatcg tcattcatta tgcgactgat ttgatagtga
      961 atctgtttag cagaaggtgt atgagaacaa tttttaaagc cctcagctgg tagaaataat
     1021 tctttctgat ctgtcttttt aactaaaatt gttttacttt tagtactacg taaagttcta
     1081 ggattcacat cagtatgaag tttaatcgtg tattcttgat catcttctaa agatattgtg
     1141 tttgcataat gcctgtctac ttctcgcaag aaatcatcat atttaatgat ttttttagca
     1201 ccctcatgat cactaaactg cttttgtgca atggaatcta attccacaaa cggtacattt
     1261 aactccccag ttttataatc aactccacta gctaacgcta taatttgttc cgtatctaga
     1321 tcaacaaaat tattcatatg tgtttcttct aaattctttt caagtcgttc aaaaacagat
     1381 tcaaatgaat attttttccc taactttctt gcactaaaag tacgttcgac atactgactt
     1441 ttttcttttc tagattgttt ttctcttcct aaagtaatta gctcatctct acgctcatag
     1501 aaatttacat cttgtttgta aagaacatag ccaagtttgt tatttgtttt tgaaaattga
     1561 taaacttcga tgttgtattc ttttgctaaa tgatccgaaa aacgcacaaa tgaatttact
     1621 tcacaattct ccaacgtagc ttcaatcgca ttttgtacaa actcttgata attaattttt
     1681 ccatttttca gtttacttgg atcgtttgaa tagagattcg ttctcttttc taaattttca
     1741 tagtactgat tgtctacagg ttcgagtcca ttttcaacaa gcacctcatc gttaattttt
     1801 cgcattcgtt ctaaatcttt agggtcaata cgcaacctct tacaggtctc catatcaata
     1861 ttattaataa caagatgact atgaaaatga tctttatcat tgtgacttgc aacaataatt
     1921 tgcgaatttg gaaaagcttt ttctgcaaat tcgacagcta tttcgtgcat tttctcaata
     1981 cttaactctt tagaaatatt cttatcaaac gaatggataa catgaaatgc ttgtgctata
     2041 tcttcagatt tattcatcct ggttcttact aaattcattt cttgcaatgc ttcctcacta
     2101 ttatcactac aatttattcc tgagactgca acgcattttt ctttttttga aggattagct
     2161 gcatattgta atagcgagga taaatttttc ttgcgtcctt tcgatgttat agtagctccc
     2221 aaagttgttt cacctcatct tttaaacttg caataatttg aatcgagtga attaaattcc
     2281 ggtcaacttt ttgggaatac tcttctttcg tatcgggtct agtttgcttt tctaaccaat
     2341 attgaaaata atctttttct acttctgaca gtttgagaaa agaagatgta ctccgattgg
     2401 actccaaaaa aatgtcatct ggataactaa tcatcatttt ttttgctatg ctattcgcaa
     2461 gttcatcttt aaaaatgttg taacggctaa cgtcaaaact aaaatgcagg ctcttttctt
     2521 tcttattttc agattgttct ctaaaatatt tagcaatgat atttaatgct tttaccgctt
     2581 gattcaaatt gttaccaatt tgatttaact gtttgattag agctacccta tcatcttcag
     2641 ccaataacgg cggttcatat ttgagtccct ttaaccgctc acgacaaaca tctgctctag
     2701 aaattcctct ttgttttgca atcaatgtca gttcaagata agtcttttta tcgacacgaa
     2761 aatttaaggc ctcactatgt ttgcgatttt tttcgtacat tttttgttta tcatccattg
     2821 attatcccct cacatttttt ctagagagca acatttgaaa ttgctctcta tttcattttt
     2881 catcattaat tcttcttgaa tcatcagttc aatataattt gtgaccgtga ttttttcatt
     2941 tgtcgctttc tttactgagt cgcataattc ggcaagttct aagaaatcct gcgtactaat
     3001 ctgtagaatt aattgcatga caatcacctc tttatttttt aaatagacta tcaaacagcg
     3061 gatttggttt atcagaaata tcctgaaaca gtggattatt ttcataagta aatcgtggac
     3121 gtttcttctc cttcacttct tgttctacaa tcactttttc gttaggcctt tcttttcgtt
     3181 cctcgataat atttattttt tgcgtcttat ctccctgact tgcttcagct cttgtttcac
     3241 gtacttctac cgttcttggt tgatttgacg cactactttg tacattttct cgtacatcta
     3301 ctgttcgatt tccattttga ctacttgtag tattctgcaa gttttctcgt acatctaccg
     3361 ttctcggttg acttgtccca ctactttgta cattttctcg tacatctact gttcgatttc
     3421 cattttgact acttgtagta ttctgcaagt tttctcgtac atctaccgtt ctcggttggc
     3481 ttgtcccact actttgtaca ttttctcgta catatactgt tcgatttcca ttttgactac
     3541 ttgtagtatt ttgtaagttt tctcgaacat ctactgagcg cctatcttga ctacttgtag
     3601 aattttgtag attttctttg actgattttt ctaatccggt atttgaattt ttagcttctt
     3661 ggattctttc tttagtcaac gcttcttttt cgtccatatt agcgcctgta cgcccttcta
     3721 cacgtttcct taacatttcc tcacgattca ttgcttcagg cgtgtatttt gcttcctgtc
     3781 gcttctgatt aattaatact tcagcagact tagcttgacc gtgcatagca tttttacgtt
     3841 cttcaatatc ttgtttaact ttttgctgaa ttgcacttgc aggattaata tttgattcag
     3901 cattattttt cattgcttca cgcatacgtt cagaactaga cataattcta tcagcgtttt
     3961 caactttagc gccatctttt ctatccgaaa gacgtgttgg catagaatca gcatattttc
     4021 cagtttcatc ttctttgttt ttagctacta taggtttgct atttttatta atagcgtaca
     4081 cggtaccatc atctaaacct tctttgaatt tattcttggt atcattagcc atatcacgca
     4141 tactcttaaa tggttttgtt gctctttcac tggccattgt agctccatcc gaagctaagc
     4201 ctgataatcc tcgttcacga gcatacccaa gtgttttccc cgttttatta gctttttgag
     4261 aaagtgattg accaaagttg cgttcaggtt ttcgttcttc cccttccgct tgatcgtttc
     4321 caccactatt tttcccacct ttagatcggt tcataccacc tgttccttta cgaaacagca
     4381 tagctcccat gccaaatgct gaagcaagct ggccatagcc ttcttttaaa ccaatatcca
     4441 cgccaaagta acgcatgact gttccactac cctttatcag cacaataaca gcgcagacgt
     4501 acgctactgc ttttataaaa ggatttacag aagaaccgct acctgttccg agataattaa
     4561 caaacattgc aaacatagac aatccgaacc cttgaaatcc gactgtcaaa taacactgta
     4621 aaatatctga caaaacttgc tttgatcgtt ggcctgtttc caagtcagta gcaaatacta
     4681 aaactccaag tacgcgttta aacgctagct cgagtattgc tgtaattata atgaaagcac
     4741 taaaaacata cgctaccgct aaagcaatca atccaaaaga aatcccccat agatttgctt
     4801 gatagcgata atatcctgat ttaatgccct caacaaaggt cgacagaaaa ttatccgaaa
     4861 atttggtagc aacaaattca ttatcagaat tttcaactaa ttcataacgc agattatcag
     4921 ctattgggtt ctctttttcc attttgtcga gtacattatc tgtcaatact tgcgtaagat
     4981 ccgttttcca aaaattttct tttgttagat tgttccggtc attcgtttta tcgatcatgg
     5041 aatattgatc tgttttattg atataagcta aatcagtaac cccttgttca atcagcgacc
     5101 acgccacgcc ttcgtccgaa tttgtaatcg tagtcgtatc attataaaat gttttagaaa
     5161 actctattcc gctgtgaatc aacgttggca ttaaaagaat caatgaaatt gacattacaa
     5221 cattcattcc aacagatttt agatcaaccg ttcccttacc tgtaatcatt ttataaccaa
     5281 taatcactag tgaaagtatc attaagccaa caacaatggt acttaaaaca gcttgataga
     5341 ctttgttcaa tccggtactt tctacaaagc taaacaaatc taaaacttcg ggaatcatat
     5401 tttgaatgcc atcgacaaca gcgaacattg attttataac agaccagcct atccacatac
     5461 cgaactggct gtaaaaattt ccgttgtatt ttttcaaata attctggtaa agcggattag
     5521 ttaaaaactc agtttgactc tcagtatcat caaaccaatt gaaaatactg ccttctgcaa
     5581 tgttactcgt aaaaagaatc acagctatta gaacaagaaa aataaccggt aaccaatttg
     5641 tttttctatt agcaatcttt tttttaatta ttttcattgt tacctccctc agcccatttt
     5701 cttaggctct cagagagttt ctgagctatt tttctgtcaa ttatgatatg agttacgtaa
     5761 tcatcattaa cagcttttaa atcgagaaca atagtatttg agcagatact ctcggttaac
     5821 tcaagagtat ctgcgttttg atcaagacag tgaatcttat ctactagatg attttcaatc
     5881 attttaaatt ccctcttttt tttgtagctt gattagttct aacgctttgt tttttagaga
     5941 agtaaagata taattatttt tttgtttgat acgatgatct gtttttgctc ttcgtatcaa
     6001 gtcacgtaaa gtcattgcta gctgttctct aacatgatct acaaaataac tattattaca
     6061 aatttcatag ataattttca gttgaacttt ttgttctttt tcagcagatt taattgcata
     6121 attgaatttt ttgtaccagt tgtcgaattg acgataatca ctattgctag ccagttttaa
     6181 gcaattcaca atactttctc cccacatttg tttaagaaga ttttcttcaa taaatgtttc
     6241 gatcaattct tcatctaatt tattattttt agttgaaagt gattcaaaaa gatcatcatt
     6301 atgtgattct ttaatatctt tactatcttt ataagactta tataattcta gttcattttt
     6361 tgaactatca gcaaaattgt ttttgtctct tgttttcatt gatgtgtcgc atagttcaaa
     6421 ttttgaacta tcaacagaat attgatttgt tccatagttc aaattttgaa ctatcaagtt
     6481 tttttgacta ttttcttgat gggttatctt attaatttta taaacatcat ctttgcacag
     6541 tacaggcttt aacaaataat aaaaatttgg gaacatttgc attttatttt tattgaagcc
     6601 attttttctt ttcacaagaa gtttaacttt ttgaagttca tctagtgctg ataagattgt
     6661 attttttgac attcccgaaa tttctacgag atttttgatt tcaaatatca aaaaaatttt
     6721 ttgattctca tcatacaagt tatttttcaa tgaaagtcta tttctatctt ttagtaacat
     6781 ataaactaac ttagaactta gtttcatttt cttatatttt tcatttacgt ttaaaaaaat
     6841 aggatattga tagtataatt ctgcatatat atcagaatta gaatagcctt gcttttcaac
     6901 taagctttca acttcttttt cattcattgt cacacctgct tctgatttta tttgctataa
     6961 tagattcaaa taaagttttc tcagaacttt attattgagt gttcccccga tctgcaaact
     7021 gacgggaaca ctctttttaa ttttttctac ttcatcatta ttcaaaaaaa cacctcgttt
     7081 tttaaatttg atgactcaat atccattttt tgcattcttc ttttgaaaac aactccattt
     7141 tttctaataa aataactggc aatcccattc ttttaaattt caaaaaagtt gtatatgata
     7201 cattcaaata tttagccatc tcttttcctt ttagaaatgg tctttctaac ccggtatcaa
     7261 ttcttgcttg atcaatagcc tcttttgtca tttcaaaaac ataatttttc aattgattcg
     7321 tttgttgttt atctaacatt actgtaattc ctgcatccat tatttttctc ctctcatttt
     7381 ctatttcttc aatcagccaa ctcgccaagt tgtatttatt tagatttcaa atcattaagt
     7441 aaccaattta ccaacttttg ataagtagat gctctaatac tttctttgct cccattttcg
     7501 attttgacat atgttttccg atttatacct atcaattttg ctgcatcgtt tttagtaagt
     7561 ataagatcgg ctctatgtct tcttaagctt ctaatgaatt gatcattaag agttatcatt
     7621 cagcgcctcc ttttaaatta ttcactttgt ggtttttatc ttaataagca cattttaaac
     7681 cacgaaacga gtaatgtcaa ttaaaacatt actcaatttg tggtttttat gctataatag
     7741 tttggaattt actatagggg gtgttacttt gaatagaata aaattttata ttgattccag
     7801 taaaaagacc attaaacaaa ttagtacaga aactggtatt aactatacaa ctcttacgaa
     7861 ctattaccgt ggtgagcgaa atcctagaaa tactgaaaca tggaatatcc ttgctgatta
     7921 tttccatgtt cctgtaagtt atttgatggg attagacgaa acaaattcca tagaacaacc
     7981 taatcttgat agaataaaaa ctgctcgttt agaaaaaaag gacaaaatag tcaagaatat
     8041 tgccagtctt gactcagatt attttactga attatctaat ctatactttt ccatgacttc
     8101 tttacttgaa gtcaaacttc aagaagatga ctccgacttt ctttttttaa aaagtattct
     8161 agaggactcc tttgaataca cgattctatt tgccaaacaa aagaagttta ttaataaagc
     8221 tttaaactta aatatcgatg aaagaaaaga gttagaatct gagttatttg gcaacttaac
     8281 ccaagtacag gttaacaatc taaaaagaga gataacagct cgattaatat caaaacttca
     8341 ataaaactaa ttattcaatc agccaactcg ccaagttatc agattggaga gattagttat
     8401 gtcaatacaa aagtacacaa caaaaagcgg tgcacgatat agtgtccgct tgtatgttcg
     8461 taaagatgaa aatggaaagc aagaatacta tgtaaaaaaa gggtttaaaa ctgaaaaaga
     8521 agccaagtta cacgaagcaa gaaaaaaggt cgagattgaa gataccggct atcataagcc
     8581 ttctaatgac ttgtttataa cagtgtatga agaatggttg ccttcttata ggaatcgtgt
     8641 acaagaaacg acatatcaac gtacaaaaga ccttttccgc ctgcatatac taccaaaatt
     8701 cgggaataag tccatttcta agattactcc tgttcactgt cagaatgcag tcaatgaatg
     8761 ggctttaaaa ttcaagaata tcaaacagct taagtcttac agctctcaaa tatttgagta
     8821 tgccattttt gcagagctat tacaccgaaa tcctatggca aatacaaatc taccaaagcg
     8881 ggaaaagaaa gaaaatgaaa attacttttc attagaagaa ctaaaagctt tttgggatat
     8941 tctcaataaa gaagagcctt taaaacatat tttaatcttc cagcttttga ttactactgg
     9001 tatacgtaaa ggagagctag ctgctcttca ttggtctgac atttattttg aagaacagct
     9061 aatgtatgtt agaaagagct atgcaactat tcgaaatgaa gagaagaatg caagaagaaa
     9121 aactgtcagg attcaaaaaa atacaaaaaa tatatcttcc gaacgaatat taccaattga
     9181 tgcacaaact attgaaatgt taaaaatctg gaaaaaagaa caagctcgag agcttttgca
     9241 gtttggcata aatacaaata caaaaaatca acttatcttt acctatgtaa atgcagacgg
     9301 tgacattaat caacctttgc atgcggacta ttcaaataat atattgaaac gtttagagaa
     9361 gaaatataaa tttaagcacg ttactattca tggattccgg cacactcacg ctactcttct
     9421 actagaaggt ggagcaagta tcaaagaaac acaagatcga ttgggtcata aaaatgctga
     9481 aactaccctt aacacttatt ctcatgttac tgaaaaagct caacgaaatg cgttagaaaa
     9541 attcattgct tacactggtt tataattttg tcattacctt tctcattacc agaacgaaaa
     9601 aaagacacct tctcgcaatg agaaagtgtc tgaaatcgtt ggtataacaa cgattaacgt
     9661 tttgaaaact gtgaagcttt acgtgctttc ttaagacctg gttttttacg ttcgaccata
     9721 cgtgcgtcac gagtaagtaa gccagcacgt ttaagagcta aacggaagtc aggatcaact
     9781 tctagcaatg cacgagcgat accatgacgg atagctcctg attgtccagc gtatcctccg
     9841 ccgtttacgt taacaaatac gtcatatgcg ccttttgttt cagtaacagc gaaaggttga
     9901 ttgattactt cacgcaagtc agcatgtgga atgtattctt caacatcttt tttatttaca
     9961 gtaattttgc cagttcctgg tactagacgt acgcgagcaa ctgcgttttt acgacggcct
    10021 gtgccgatat attgaacttg tgccaatgaa attccctcct attagattaa gtttgtgatg
    10081 tctaaaactt ctggttgttg tgctgcatgt ggatgttctg ctccaccgta tacgttcaat
    10141 tttgtaaatt gtttgcggcc taaagtgttt ttaggaagca taccttttac agaagtttcg
    10201 atcaaacgac gagaattttt agcacgcaat tcgccagcag tgattgattt caatcctcct
    10261 gggtagttgc tgtgacggta ataaattttg tcagtcgctt ttttacctgt taatttcact
    10321 ttgtctgcat tgatgacgat gacgaaatcg ccagtatcca aatgaggtgt gaaagttggt
    10381 ttatttttac cacgtagtac agatgcaaca actgttgaaa gacgtcccaa tggaacatca
    10441 gttgcgtcta ctacatacca tttacgttct acttcgcctg gtttggccat atatgttgta
    10501 cgcacgttat tttcctccaa tttaagttct gatgtttgac atacacttcg agtttccggg
    10561 gctctttgtg gggcaaacaa taccatttaa catattaccc gctaatctag tcaatgtcaa
    10621 cgtttttctg cttgattgaa aaatttatat gaaactttac ttcttttctt tcttttatat
    10681 aaaagggtcg tttcctgtca aaatgaaaaa caaaaagagg ctgtgacaaa agtcttgtca
    10741 caacccctta ttttgaataa acggtgttct cctgccaact cctcggtcac aagtcacaat
    10801 atccataaaa cataagaagc tgttttttaa aattgtttct tgtgactcgg agctgaacgg
    10861 caggcttaca acctcttggt aaacggtgtt caatcagctg accctcggta ttagctcaaa
    10921 aaacggcaaa atatgaggaa ctattttgac gtttgtttct ctaatactca ggtcaaaacg
    10981 ctgatttcac aacctctttt tcggtcaagt tcaacaacag tttttacgcg tcgatctctg
    11041 gcgctttgcc caaatgagct tggatcatcc agatacgttt ttctgtggct gttttgaatt
    11101 cgataaagat atcttgtgtt ggatcgtcgc cttcttctcc agaaacttcg attccttttt
    11161 ggtaaagatc tgctagataa cgatagcctg caacaagtgt ttccaaacgt tctggaatag
    11221 tacgatccca gcggccaatt tcatcaggga tttttgtgtt atctgcaaat tcacgcaatg
    11281 tagaataagg tgaaccgtct aatgtgatca agcgttcgga tacttcatct aactgagcgt
    11341 tcaaatcatc catgaactca tccatcattg ggtgcaatgt aaggaaacct ggtcctctca
    11401 tgtaccaatg tgtttggtga acaacaacag acatttggct tagatctgca actaattgat
    11461 ttaatacttc tttggtttgt tggaatttca tctttcttgc ctcctaacat tttcttacac
    11521 ttaaaataat agtagacttt caatgaaaag tacaacaaaa tcatttacct gagaacgatt
    11581 ttcagttcgc ttttttatcc gtaaagccaa tcatttttca gaatcagcta attcttctcg
    11641 cagttgagca aattgctgat aagtctgtgc taaatgcttt tgtgtcgaaa caaataatgg
    11701 atagattttc tgatattttt tatgatcagt ttcttgtact ccaacttgct ctttcaattc
    11761 tatcaattcc tgtgcttcac tgatactttg gatctttccg atgcttctcc agcctaaaat
    11821 cacagctcct aatgcggatg cttctgtgac agtagggaaa acaagcgggt aacccagtac
    11881 atcagccatc atctgacgaa aaatcggaga ttgagcaaac ccgcctgtag cacggatctc
    11941 tgtgagctga ttttcctggc aaactccttt taaaatccga taaaggttga gacagatccc
    12001 ttctagaatc gccttcatca tttctttttc tgtatgttgc cgctgtaaac cgataaaact
    12061 gccaaacgca tctgcgtccc aaagcggtgc tcgctcacca agtaaatagg gatgaaaaat
    12121 cagaccattg gcacccgctg gcacaaaagc gatagccgaa agcagtgcat catatggatt
    12181 ttctcctttt tctttagcag attgaacgac ttcctgtaaa aatgtatgcc ctgcccaatc
    12241 aaagatgcca gcaccattgc tagtcgctcc tcccactacc caatgtttct gatccagaac
    12301 ataacaaaac gtctccattt ctggatggaa ctgaggctga gtagtaataa aacgaagtgc
    12361 tccgctcgtt cctacggtca aagcggcgat cgtatcacca aaagcaccta accctagatt
    12421 agaaagcggc ccgtctgccc ctcccaaaac aaatgatata tcatttggca aacctagttc
    12481 tgctgctgtt tttgatggta gacctgttac ttgataggtg gaaggaaaga cttgcggcaa
    12541 ttgctctatt cgaatgttca agtagtcaag cacttcttct gcccattgga attcatggat
    12601 atcaaaatat ccagttcctg aagctgttcc gtagtcgctg atcagttcgc caaacaatct
    12661 ataaaagata tagtctttga taccgacaaa ataagcagtt tttttgaata attcaggttg
    12721 gctgttttgc agccacttga ttttgttcaa tggtgtcatt ggatgaatcg gcgtcccagt
    12781 ctgccgataa aattctttgc caagcacggt atatttcagt ttttctgctt cgtccgctgc
    12841 tcggttatct gcccaagtga tcattcgaga taacggtgtt ttctcttcat ccaacaatat
    12901 caggccatgc atcgcagagg agaaggagac aaattgaatg attgtgtctg aaggcaactg
    12961 tttaattact tcttttataa cggtgcagac accttgaaag atcaattcct gatcttgttc
    13021 tgccatccct tgtgtttctt ggatcaaagg gtagcgaaaa taagcggatg ctgcttcttg
    13081 ggcttcatct gtgaaagcaa ctgctttggt ttgtgtggtt ccgatatcta atccgattga
    13141 aacgttcatt ttttcacctc aaattttatg tatgtacaaa aatttagttt aagcttgcgt
    13201 atcttggttt tgaaaagggg gagatggcgt gttactttat tttatcattg gttgctgtat
    13261 cggatcattt ttatgtttaa ccgcccagcg gattccgtta ggtcactcta ttatttatcc
    13321 tagatctcat tgtgtcaaat gctgtcgttc gctttcttgg tatgaattga ttccgattct
    13381 ctcgatcatc gtccaacgat ttcgctgccg ctattgcagg tgtcggttgc ccttctacta
    13441 tttgttggca gaagccttat gtggtggtct ttttgcctgg ttctttactt tttcttccgc
    13501 aagaaatttt tcgacgttca tatggatgct ttcggctttg ctattttcgt tgatggattt
    13561 attttacttc atggtccatt ctcacacgct attctgttct tgggcaattt tatggatttt
    13621 ttggttgcag gccggcgtct tccaatggca aagtgtgctg ttaacgttta tcgtaggcgg
    13681 tgtgtgcctt cgctatggtc aatcctatct cggagctggc gatactctgt tgatgctttc
    13741 ttggagtggt ggcttgtctc tagaagagtt gcttcagatc ttgtttcttg ctagtctgtt
    13801 tggaatatcc gttttttctg tttactttat ctgtcataaa aaaaagttgg aaaagctgcc
    13861 tttcattcct tttttgagca gtgcgttgct gattgtcctt catcttaggt attgaaaaaa
    13921 ggttgtgaca aacgacttgt cacaacctct gatatatagt gttctcttac caaatccttg
    13981 tgacttggag ctaggcgaca ggttcacaac ctctccacta aatggtgttc aatcagctga
    14041 ccctcagtat tagctcaaaa tgctggtttt acaacctctg atatacggtg ttccataagt
    14101 agctttttcg gcttcttatt tcttgacgcc ccaatgtcga cgaaggaaac ctgcacgtcc
    14161 agaaccttct cgataaagat tgtaatggag cttgtttttc ttgtaataat cctgatggta
    14221 atcttctgca ggataaaatt cgcttcttgg ttcgattttt gtcacgattg gcttatcgaa
    14281 tcgcccgctg tcttgcaatg cttgttttga acgctctgca atatttcgtt cttcttcatc
    14341 attatagtag atcactggac gataagaatc accgcgatca gcgaactgtc ctaatgcgtc
    14401 agtcggatcg gtttgctgcc aatagatttc caccaactga tcataagaaa taacttctgg
    14461 atcatattcg atctctactg cttctgtatg ccctgttgtg cctgttgtta cttgttcata
    14521 agttggattt ggtacatgcc cgccagtata accggatgtc acagaaatga tccctggctg
    14581 ctcttcaaat ggtttgacca tacaccaaaa gcatccgcct gcgaaaactg ctttttttgt
    14641 catataaaat cccccttttt tctcatcata ccttgatttc caaaaggagg ataacgtttt
    14701 gcttgtgaca gaaaaaacgg aaactattat atacgtgata cggcttctga taaatgccaa
    14761 aacgaacgta cttgatcaaa aacggttaaa tcaggaatca catattcagc agaaggaaga
    14821 tttatgtttt cacctattcc caatacatgc atgttaccag ccaaagctgc ttgacaacca
    14881 gcttctgaat cttcaatgac caagcatgca tttggcggta cattcaattg ctgtgctcct
    14941 ttgagaaaaa cttctggatc aggttttgct tttgatactt gcgtgccgtc aataaggaca
    15001 tcaaaataac ttgtcaaatt cgttttttct aaaatgaggc gtgcattttt gcttgctgat
    15061 cccaatccga tcttgatgtt tgtttttttc aagtactcaa gaacagctat tgcaccaggc
    15121 aaaacggatg tttcatccat tttttgaatc gcttgaagat atagattgtt tttttgctca
    15181 gcaaaggctg atttctcttt ttctgagaat ttctgttcca tttttccaaa tgacagcaat
    15241 cgctctagtg actcggttct ggatattcct ttcagttgct cgttttcttt ctcagtgaat
    15301 ggaatggaca gctggttagc taaaaccagc cacgcttcgt aatgataacg agctgtgtct
    15361 acaagaacgc catctaaatc gaatagtgct gctttgattt tcatcgatta cctccttgaa
    15421 aatgaacgac ttgaagtaaa gatgcgatgt ttttagctgt gcgatgaaga ttatcagtgg
    15481 cttgctgtaa agcttcttgt aaaggcacag ctgcaggaag gatcggaaaa acggccgaaa
    15541 tgccaacatc tgctgctttt tcgcttcctt taccaacact tccgcaaata gctatcacag
    15601 gaacgccctc tggtactctt ctagcaatcc caactggcgc ttttcctttt agactttgtc
    15661 catctagacg tccttcgcct acgattacga ggtctgcttg ctgtacccgt ttatcgaact
    15721 ccagtaaatc caatacgtag tcgatgccag agactatttt ccctgaagca aatgctaaca
    15781 gtcctgcacc cattccacca ccagcactac tgcccgatgc ctcgattact tgaggggcaa
    15841 atctcatata aaaatcatgc atggcttggt cagccactgc taattcattt tctttcaagc
    15901 ctttttgtgg tccgaaaaca aaagaagcgc cttcttttcc gcataaaaga ttagtaacat
    15961 cagtaatgac agtgattttg gctttaatca gctcaatagg cacgttcgtt ttatcaactg
    16021 tagcaatttt acctagattt tctcccagtg cttctacttt ttgattatct tgatcgtaaa
    16081 aagcataacc tagaccttct gccatgccga tgccgccatc gttggtacta cttccgccaa
    16141 cgccgatgat gatgtgtcga acaccttgtt gcaacaattg tcgtatcagt tctcctactc
    16201 ctcgcgtgga taacctaagt ggctgttgtt ggtttagtgg gacatctttc aatccgacga
    16261 tttctgccat ttcaaaaatc gcagtttgac cgtctgaagc gtactggatt tctttgtttt
    16321 tgccaaatgc attagtaacg actgctgttt gccgcttcaa ccttaaactg tctgctaatg
    16381 atgaaaccgt tccttctcct ccatcgccta taggtagcag atcatattca gcctgaacaa
    16441 aaacttcttg aaaacctgat tgaatggctt gtgctgcctc taaggctgtc agactttctt
    16501 tgaaagaatc tggcgcaatc agaatacgca ttattttccc ctccgttcca agatcagttc
    16561 tttttccagc tggtattggt ctccattgac aaaaataggt aatgtttctt tcgtctcttt
    16621 ttttattact atttgttttt ttgtcaaaac aaatagcaac ctgtgatttt tccaaataaa
    16681 aggaaaagaa attttttccc atgctgaagg aagtcttgga gaaatagaaa gagttccatt
    16741 cgttactgta atacctgaaa aaccaaatat aactgctagc catagtgctc ctaatgaagc
    16801 agcatgaatt ccgtcatctg atgaatgcgg atctggtcct aaatcaatca ggcacgcttc
    16861 ttggaataaa tgatacgccc attccatttc ttggatacgg caagcctcta tggcgtgaat
    16921 cgctttactc aacgatgagt cgtggattgt tttgttttca tagtaatgca aattcttaga
    16981 aacgattggt tgagaaaaat actcaggtaa taagtataac agcatgacaa gatctgcttg
    17041 cttgctgact tggagttcat ttacttcgct acgggcataa tcaagtaaaa tagactggct
    17101 gccaggacgt tctttgtaag tggtcaaatc aataactggc ttactgagat acgtgtcatc
    17161 ttgcggaatc agctgttctt tgttaggcac tggcaaatat aacttatcta aaaaattttg
    17221 acattctttc aaaaaaagcg gatcgccagc ttgatattta acagctaagg ccacattata
    17281 atgcgccaga tagttcgtat atgcattatt gtcgatatat tctgtgtatt catctggacc
    17341 aatgactctg tgtatctcta aatgatcatc gacttttaca gctctgctca gccaaaaagc
    17401 tgctgtttca tgaatgagcg tttttccctt tgtttctaaa aacgattgat cctctgttgc
    17461 ttggacatac tcttgaacgg cataagcaag gtctgcactg atatgatgtt ctgcggcagc
    17521 tgaagcaact tcttgtcttg ttccggtttt gatattcaat gcagcaaatt tgggtgtctc
    17581 ttcctgacca gtgtgtgcgc tttcccatgg gaaaagtgca ccttgatagt gataatctct
    17641 tgctttttgc cgtgcgcctt ctaatcgatc ataacggtac agcagcaaat tctttgctac
    17701 ttcaggaaac tgatataaaa agaaaggcag gataaaaatt tccgtatccc agaaaacatg
    17761 ccctttataa ccttcgccag ttagtccctt cgcccctaca ctaacacttg gatcattgcg
    17821 gggagtcata atctgtaaat ggtaagcagc gaagtccatc gctaactgat cgaaagaaga
    17881 atcgccttgg atcgttatcc gatgctgatg ccaaaaatct gtccaataag acatcgactg
    17941 ctctaaaatt tctgtataac taacgctttt ctgggcttgg gcaatacttt tcttaaacgg
    18001 gtctgtttgc tgaagagagg tagagagcac gatgatcttc tccagcgtaa aggacgtatt
    18061 ttttctgatc ttttctgaaa aggaagcggt gatccggcga ttttttgctt ggaatacacc
    18121 tgctttattg aatttagctg caatcccaat atcaatttta ctttctgttg tctggtagga
    18181 tgcactgatc aaatcgtcac caaatactcg cagttctttt tcaaccaact gctgtgtacc
    18241 aaaattggtt tgctgtccat cgatacccgt tttcacatct acttggcagt cttcggtcaa
    18301 cggcttgatg accactttca gggctgcctg atggagatta ttttgatcag caacacgaca
    18361 agtaatgact tccacttttt ttccttgagc agtttcaatg agcgcatagc gtttaagctc
    18421 tcccatttct aaatctagtg cccgctcgta tgcaagaact ttcatcgtct caagagaaaa
    18481 atgttcaccg tcgattttta tttcccatct gatgacatct gggagattca ctaactcggc
    18541 tgattctgct cctaaaggtc ggttatatac accagctgca aacattcccc ttacttgatt
    18601 ccgatagtct tcctcctggc ttcctctgat accaaaaaat ccatttccaa tggtcatctg
    18661 actcgcatac ttattgatat actctaaatc aaactgtttt tcttctactt tcataaggta
    18721 ataacttctc ctgtttctgc cgaacgatat aaattttcca ccagattctg gatgacatat
    18781 ccttgccagc catctgcaat catctgagtc tggtcaccga gcgcccgatt cacaaaatga
    18841 gccatactta gttcatgtgg gtcattaact ggcgtggtct tttgtaagga aaccagcttt
    18901 ccatctacat cttcgaatag ttcgcctgga tacagattag cacctgcctg atcgccacaa
    18961 aaagccacat tcagtttgtc ttctctgata ttcaaagcaa acgaagtatt cagttggatg
    19021 atcttgccgt tttctaattc cagtgtgcca aaaattgcat cttctactgt gaatttgctt
    19081 ggatcccatt tcccgaattt ccctgaattt tttttcgtac caattttttg gaagctgtac
    19141 gcccgaacgc tttttacttt aggaaatcca gtaatataca tcgctgtatc aagcatgtga
    19201 atgccataat cgatcaaagg cccgcctcct tgaagttctt tgtcgataaa cgttccccag
    19261 cctggaacac cagaccgacg aagcgcattc gcttctacgt aataaatatc cccgagaaca
    19321 tcaagatgat tcttgatcat tgttgcttcg tcagaaaaac gatgctggaa tccataagcc
    19381 aaaatctgcc cgtttttatc ggctgtctct gccatctttc ttgcttgatc agctgtcata
    19441 gctggtggtt tctcgcacat cactgaagca ttagcttcga gggcagccaa tacatgttcg
    19501 aagtgaaagc gattgggtgt acagacactc acgatatcgg gagaaatctc attcagcatt
    19561 tgtgtaactg atgtatacgc atgagaaaat cctgtacgtc ggcaaaagtc ttctgctcgc
    19621 tggctgactg gatcaaccac agcggccaat tctaagacat ctgatctttt ttgataataa
    19681 gcagcatgta ctttttctgc tacttgtcca gcgcctatga ttgctacttt cttcatttat
    19741 ttctccctcc tttgcttatt tacttattgt tattctttct ctattctttc tctaaatcat
    19801 ttaaatgacg cgctgtttcc agacagtttt tcaagtattg caatgattcc tgataaattt
    19861 tcacttcatt ttctcctcgc aaacgacatt catataccat ataaccttca tactcatttt
    19921 gaagtagcgt ttgaaaatgg cgagtaaaat caattgcgcc agatcctggt tggtagcgat
    19981 gattatctgc taaatggata tgacccacat actctttttg cgtagtcaat gcttttgaaa
    20041 tatcatcttc ttcaatattc atatgataaa aatcagcgat gatctgcaca tgtttcaatt
    20101 gattagagtc aatgtaatga cgcgcatccg ctaacaaatt gatcatatgg tcttggtatc
    20161 ggttcaaagg ctccaaataa ataaccgtac ctgtttcttc ggctacttta tctaaataac
    20221 gaagtgattc gctgactgct ttatagtctc cttctttgct tcggggtgaa accatcggcg
    20281 gtaatcgata agtgaacatt ccccaagctg ctgggataac gattcctttg cctccaacct
    20341 cgcttagtgc acgcaaaatc tcagcaattt gacgtacacc attaagacgc cgttcttcga
    20401 taaagtcacc gatccaccca tcgtagccgt tacaagctgt cactactggg atccctgttt
    20461 ctttgattgc ctgtttgact gtgtcgatat tttctattaa caacttaccg tcgatttcgt
    20521 attcttcaaa tccacagtct ttgataaatc taaatttttc taaaatgtct cttgggaaaa
    20581 atgcttgatc ttgcgtaccg aatttcattg ttctcctcct cggacttgaa cgcccatttt
    20641 gatactttct tctgggtgct ggtctacata acgcatgtaa gcttctgcgc tttctgcaaa
    20701 tggcacaact ggatcaatga tttcttcaca atccaagtaa ccgttcatca ataattccca
    20761 acatgtgtcc tcgattcgtt tacggttcca tcttgggtaa tctgggtttg gttcactaga
    20821 tgctcgagca aagacgattt ttgcattatt gaaatgtgct tcgcgaccaa gattaaaccc
    20881 agctggaaaa ggtttagcaa aggcaacata agaaatgatc ccgccatagc cgatccctct
    20941 taaagaagct tgtaatgcgg cagcactgcc actcgtttca ataactgaat ctactccacg
    21001 tttatctgtc aattccttta gacgaaaacc gacatctgct ttcataggat ctagcgtgta
    21061 atcggcaccg tatttttctg ctaattctct acgctgcgct atgggatcga tcccaacgat
    21121 ggtagtagca cctgcttttt tagctaattg aatcgcaatt tgcccgatag cgcctaaacc
    21181 gaagattgct acagcatcac ctgctcgaac atgtgcttcc cggataccac ctaaagcgaa
    21241 ttgagcagga tcgtaacata ctgcattttt ccattgatcc aaccgatcaa gtttacgtag
    21301 tttataatta tcaacaccgt ttgtaatcag cgtttcacga atgggtccat aagtgcagac
    21361 taactctcct ttttgatact ctgtcaccaa actgcccgtt tcttcgatcg taccgacaat
    21421 catatttcct aattgaaatt cgccaaatac cacaccgcgt tggcttcctt cttctcttgg
    21481 caagaatagg cgccattctt ctgaaaattc ttcatcgata aatggactta atcctctaaa
    21541 gtcgacaatt tctgttcctg gttttggagt agcgaaaatt actcggatac gtacttcgtt
    21601 cttttcgatc tcacggtctt cgtaatcttt gaatccggct attcttggtt ctaatgcaac
    21661 taattttttc atcttttctt tcctctttct tttcttttta tccttttacg ccgccttcga
    21721 ctaaatcacc tttaataaat cgttcagata acgcatacat aatgatgact ggtaaagcgg
    21781 tcaccaatga agcggccatc attcgtcccc aaatgtagtc tggtgtactg aataatgtgt
    21841 tcaaaccgat tggtaaagta aatttttcgg aggtcgacag gaaaatcgaa gcaaataaat
    21901 aatcattcca cgaaaccata aagcaataaa cgaagacaga aatgattcca gaaatagcca
    21961 atggtataat gatccgaata atgatctgga tccggctaag accatcgatc attgctgctt
    22021 cttcaatatc agtcggaatc gtgtcaaaat aactttttag catgaatatc gcagtaggaa
    22081 gtgtttgaac gaccatcgtg ataatcagtg ctgtattggt atcatacaaa cctagaccgg
    22141 aaatgatttt aaataatgga acaacgatca aaataccgga aaacatataa acggtgtaaa
    22201 agctagcatt gattttcgag cgagctttga agcgtagttt tgacaaagcg tatgccccgc
    22261 tgatcccaat cactactgca gttacggagg ctatcaagga gatcactaaa ctatttttga
    22321 aatacatgat gaatggaaaa atctcagcat tgaaaatatc ctgataatgt tcaaaagtcc
    22381 attctttcgg aaaaatagtt tgtgtcgttg caattgcttc ttgtgaatcc ttgaatgatg
    22441 tcatcagcat gatcaaaaat ggaaacaaat tgacagctaa tagcaacaaa atcgtcgcgt
    22501 aaaataagat tttcctgctc tttttatttt tcatttcaag tccaccttct ttcgtgagag
    22561 caagacgacc gctgcaataa tgatgaacaa tagaatcgat atagctgctg cttttccaag
    22621 atcattgaac gcaaaggctg tctgatacaa ataaacgcct aagatattga ctttattcgt
    22681 cagtagatag acatctgtaa acatatagaa catccagata gatcgtaaaa caatcacagt
    22741 tgctagtact ggtttgattg ccggcagtgt cacgatcttg aacttctgcc aagcatttgc
    22801 accgtccatt tcagctgctt cataaagcgt tttgtcgact gtttgcaaaa tagccaaaaa
    22861 tgaaataaac gcatacggat agtatctcca gatagcaaac aaaacgacca taaagaagct
    22921 agcaactggc tggtcgaacc acagcggtgc ttcgttgaaa agctgcaacc tatctactaa
    22981 cagatcattg acgattccat aactattatt gaacatatat ttccaagcaa aaatcaaaga
    23041 aatagatggc gtaacataac ttaataaaat cagtgaacgc accgttttcc taaatctgaa
    23101 cttgcggtta agcaaaatag ctacggctaa tcctaaagct gtactgcctg ctacaactaa
    23161 gaatgtgtaa gcaactgtta aacctaaaga acgataaaat tcactatcac ttaaaatttc
    23221 aagataattg cttaatccga taaaaacggc gtctgagtta ggattcagcg gcatatcgaa
    23281 aaaactgatc tgaatattgg aaagcattgg aatcagcact aaaacagcta ataaaagcat
    23341 gcttggtgct aaaagtccca tggctaattt taagtcagaa tgtttccttg gacgattcat
    23401 tgaaacctcc ccctgataat ctgccaatag ctatctactt ttttcgaaca ccattggcag
    23461 aacttcttgt tatttcgtat ttttttgtgc gtcagctaaa gcttgatcga catcttcgtt
    23521 tccgacagtt acttggttca ctgcttttcc gatcacacct gagcttgtga tatctcccat
    23581 tttcgtgaaa ttcttttccc cgactagtcc aaagacttgt acttcatcaa aagcactagc
    23641 aatctcagat ggcagctcgc caaaagactt gatgacttca ttttctttgt aaccatccag
    23701 ttcgactact tgtttattca ctggttgagc accaccagga gacatcaaga cccacttttc
    23761 catgtttttc ggttgagata agaaagctgt gaatgcttca gcagcctctt tttgtttctc
    23821 gtctagacca gaagaaatcg tcaatccaga tactgtgcca tagacagctt gatctttttg
    23881 tgttgggata gcaaaaccga tattttctga gttccccgct tcataaacag aaggcaaaat
    23941 gtaagttgaa tagattgcca ttggtgctgt tccattcatg aaagcatctt tgatttctgt
    24001 cacatcatta gagcctggca tcgtgtattg ggataagttt tgataaaacg acaatgcttc
    24061 tctcatcttt tccgtatcga tcgttacctc gccttcatca tccaaaacat ttgctccatt
    24121 tgacaaagca aattgagaga acgcttgctc actcattgtt ccttcagcag taggcatagc
    24181 aattccatat tgtttattct ctttatctgt gaaatgttga gcaattttta aaatatcatc
    24241 ccaattttct ggttctgaaa aaccagcttc tgataatttt tctttgtcat accaaatacc
    24301 ttgaacccag ccgctaattg gtgcagcaat atagcttttt ccatcttctg aacgaacaag
    24361 gtttttggct ccgtcataat aattatcttc tccagcttcc tcgataacgt tttggactgc
    24421 cgtctgatca atcagttcat ctttatccat cactttagca aaatcctgac tgacttccat
    24481 tacttctggt aatttaccgg aacgagctaa cgtaacgatt tttgtattga acgctgattc
    24541 ttctaccggc acttgcttga ttttgatgtc tggattttct ttttcaaaat cagcaaccag
    24601 ttcattgatc acacttaatc gttcctgttc gacagaagag tgcatgaatt gaatcgttgt
    24661 ctgttgtgat tcgttagagc tttgactttc accacaagca gtaagaagaa atccagtagc
    24721 tagcagacag gtagtaacaa taattttttt catttttttc cacctcttat tttattattt
    24781 tcagccacat atattcatac ggctggaata tcaactttcg ttcttgactg caatggataa
    24841 tttgtccaga aaacaggtct aggtaatctc cttttggtaa aagataacct gctgattgat
    24901 tggaaaaatt atggataact aagactttct cttccttcgc ttcgcgaata aaactgataa
    24961 aagaatctgc tgagtccagt aagtaaatca aactattcgg atggaaggct ttttcttttt
    25021 ttcgaataga tagcatctgg gtcaattcac catatactgc tgttcttaat ggatctgtct
    25081 gcaactcttt ctcaatatct gctaacaaaa atttttgccg attgattttt cgattctctc
    25141 ctgttttttc tacgcctttg taatcatttc gacttcccag cacactttga atataaattg
    25201 ccggcacgcc tggtaacgtt accaaaaatg aatgagcagc taaaaagcgt ttgcttctcc
    25261 attccgtcgt atctgttgga gaacttaacg catctagata cgtagcgttt atctcatatg
    25321 gactggtcgt tccatctggg tttttcttgt agtttactcg ggcaccctct ttcttcagct
    25381 gttccactaa ttcaaggatc gttttttccg gcagaatacc tcgtaccgga ttcaatccga
    25441 taccgtcatg ggaggctaaa aaattaaaaa aagttgtttt tttcgatgcg aaatcgatgg
    25501 tttttaacca ttctttcaac ttacttgtat tacccgaacg aatcgcttga agggtcaaag
    25561 gtggtagcga aaactgatag accatctgtg cttcattctc gccattccca aaataactga
    25621 tattatcttt gtgtggcaca tttgtctcag tgatcaatac cgttcctgga gctgcttcat
    25681 cagcgattgc acggaataat tggatgattt tatgcgtttc tggcaggtgt atacaagaag
    25741 ttcctagttc tttccataaa aatcccacag cgtccaagcg aataaatgaa gcgccttttt
    25801 ctaagtaaaa caaaagaata tcgatcattt tcaataaaac ttctggattt ttaaaattca
    25861 agtctacttg atcttcgcta aaagtcgtcc atacgtgttt ttctttcccg tttttgtcaa
    25921 tgaacgttgt aagtaacggg ctggtccttg gtctagtcac gctgcttgta tctgtatcca
    25981 gtggcaaatc aataaaaaaa tctttgtacg tatcattcaa gtttaaatat tcctgaaacc
    26041 aagtactttc ggcagacata tgattgcaga caaagtcgaa cattaaccgt gtttcgcttc
    26101 tcatttcttc tatatcttcc catgtgccca aacggtcgtt gattgcctga taatttacaa
    26161 cggagaaacc atcatcagat gtatatggga aaaatggcaa gaaatgtaca actctaaatg
    26221 tatctgccaa atacttttga taaaatttct taaatgtacg caaagttggt tgctgctctt
    26281 caaaaaattg atccccataa gtaatcaaga aaatatcttc ttcgtcccaa tgcagtttgc
    26341 ctgcctggcc tttcttctgc tgataaacac tgactttttg ttcaatcata gaggcggttt
    26401 tctcagacaa atctgagtcg cgataaatcg cttgtaaaag ttttgtatac atcggattcc
    26461 cctttctttt ttgggagcgt tcccaacgat ttgatcatat tatggaaaac gcttactgtc
    26521 aatagcattt ttttaaataa ttttgatata cacaaaaata caaataagaa agcttatgta
    26581 tcaataatta atagcttctt aaaaaaatat ggtctctcat gatcatgcaa gctattccta
    26641 aattttcaaa atcatagaca aaaaagaatg tctattgtac tatggaatta ttctaaaacg
    26701 ctcccaaaaa ggaggtgcat ataaaattga aacctactat ttatgacatt gcgaaaaaag
    26761 ctaatgtctc aaaatcaact gtctccagag tattaaataa ccagccaaat atctctgata
    26821 aagcaaaagc aagtgtactt caagcaatca aagaattgaa ttatgaaccg agcaaaattg
    26881 caagaggact gtcaggaaat ggttttgacg cgattctagc attatctcat cgtacttctc
    26941 atacgaccta tggcaatcct tttttttcag atgttattca atccatttct gccgtttctg
    27001 aacaaaataa cttcgacttg attctacaaa cggcaaaaac accagaagaa gagctagaaa
    27061 aatgcgcgaa aaagatccaa gaaaaaatga tccgaggaat tattatttta agctctacga
    27121 tggatgaaac acttcttttt gaattggatc aatacaatat tcctattgtg gtaatcggaa
    27181 aagtcggaaa cgactacacg aacatctact cagtggacac ggataatttc caggatagtt
    27241 acgatttggt ggaaacactg atcaagaatg gacaccgtca tatcggctgt ctccatgctc
    27301 cgacagaagt ttatgtatcc aatgatcgtc tcaatggtta ccgtcaatgc ttatttgatc
    27361 accacctaga tatccgtaat gaatggatta tagacggcgg ctttcgctta gaagatagca
    27421 tggcagcaat gaatgattta atgaagctac gagaaagacc aactgcgatt ttttctaccg
    27481 atgcattaaa atcattgagt atttacaaag tggcagaaca atctggaaag cagatacctg
    27541 atgatctttc gatcgcagga ttcagtgatc ccttattgtc ctccctattt tcgccacagt
    27601 tgactcggat agacattccg acaaatcagt taggaatcaa agccacagaa cttttattcc
    27661 aactaatcca ccaccaatac cctgaagaaa aaagaatcat tctttcaacc gagattactc
    27721 tcacagattc gatccaacaa caagaaatgt agaacagaat tataaaagaa cagaaacact
    27781 gaggataagt cagttactct gttctttttt caatcactca tccctcgtta ccccctcccg
    27841 gtattttagc gaatttttct tattttccat ctgttttaga tagctgtgga taactataat
    27901 gtttgtgtta aactattttt tccacaggga agaaaaaact atttttaacc acgttgtgta
    27961 taagttttta tagttcgttt ttttaaacac atttaccctt atttcaatcc atgttatttg
    28021 gggatactta tctctaaaac ataaatagaa taacagacat gtctactggt tttgttttat
    28081 tcgaggaatt attcaataat agctagcaaa atattaaaaa taaccgctct atcttcccat
    28141 caatttattt tgcattcaaa aaatgatcta tctttacttt tgttgattga cttctcttgg
    28201 aagatgatcg ttccgatacc ttaattggat acgaaccgat gttgttaaga tcctttccca
    28261 cttttcttct agttagacaa attattttag acaaaccaaa aaagaggttg tgacaataat
    28321 ttgtcacaac ctctttttta aaatatctat tttgtttgat tgttcattgc gtcttctgca
    28381 gccatttgcg cttcaatatc tgaaatactg tagacatttt cgcttgctac accgacttct
    28441 tttggttcca tgtcacgata acgttgcata ccagtaccag ctgggatgat cttacctata
    28501 ataacatttt ctttcaaacc aagaagagga tctttcttac cgcggatcgc agcatccgtc
    28561 aagacacgag ttgtttcttg gaatgatgca gcagataaga aactatttgt ttctaatgat
    28621 gcttttgtga tacctagtaa gacaggtcga gaagttgcag gaacgcctcc tgagactagc
    28681 gtattgtagt tttgatcttt aaagtctgcg atgtctaata atgtacctgg taagatatct
    28741 gtatcacctg gatccattac acgaacttta cgcagcattt gacgaaccat tacttcgata
    28801 tgcttgtcgc cgatttctac cccttgcata cggtaaacac gttgtacttc acgaagcaag
    28861 tagttttcaa cagataatac atcgcgaact tgcaacaatt gttttggatc gattgatcct
    28921 tctgtcaatg gtgcaccacg gtggatcata tcgccttctg ctactttcat acgtgctgta
    28981 taaggaacag tatacgtacg tgtatctgtt tttcctttga ttgttacttc tttggtacgt
    29041 gttgcaggat cttctgagat atcgatgaca tcccctgtta cttctgtgat cactgcttgc
    29101 cctttcggat tacgtgcttc aaagatttct tggacacgag gaagaccttg agtgatatcg
    29161 tctccggcaa ccccacctgt atggaatgta cgcatcgtca actgagtacc aggttcaccg
    29221 attgattgag cggcgatcgt accaactgct tctccgactt caacgtcaga gccagttgca
    29281 aggttgcgtc cgtaacaatg tttgcagaca ccatgttttg tattacatgt gaagacagaa
    29341 cggattgtca ctgtttcgac acctgcatca acgatttgtt ttgcgatgtc ttcggaaatc
    29401 aattggtttc ctgcaatgat caattcattt gtttcaggat gacgtacttc tttacgggtg
    29461 taacgtccca ctaaacgatc ttctaatgat tcgatgattt catttccctc gcggatggct
    29521 tggatttcaa gacctcggtc agtaccgcaa tcttcttcac ggatgattac atcttgggcc
    29581 acgtcaacca gacgacgagt caagtaacct gaatcggcag tcttcaaggc tgtatcggtc
    29641 atccctttac gagccccgtg agttgagata aacatttcca agacggatag tccttcacgg
    29701 aagttcgaaa tgattggcaa ttccatgatt cgaccattag gagcggccat caaaccacgc
    29761 attccggcaa gctgtgtaaa gttggaaatg ttaccacgag ctccagaatc agacatcatg
    29821 aagattggat ttttcgcttc caaaccttcc atcagttttt gttggatgct gtcttttgca
    29881 gcattccaaa cagcaatgac acgttcataa cgttcgtcat ctgtgatcag accacgacgg
    29941 aattgttttg taatagtttc aacttgttta tgggcatttt caatgatttc ttgtttttca
    30001 ttcagtacac tgatgtcagc gatccctact gtgataccgg catgtgtcga atgtttgtac
    30061 cctaagtctt tcatacggtc aagcatctta gaagtttctg ttactttgaa tcgtttgaag
    30121 acttctgcga tgatgtttcc taagtttttc ttcttgaacg gtaatactaa ttcttgctct
    30181 ttgatatgtg caggaatatc agtaccagct tcaacaaagt atttgtccgg tgtttggacg
    30241 gtcaagttga agtctgttgg ttcattcaga taagggaatt caggaggcat gatctcgttg
    30301 aagatgatct taccaactgt tgtgatcatc gtacgttctt tttgccattc agtaaatggt
    30361 ttttcaccta atgagttagg gttcacaccg atacgtgaat gtaaatgaac atatccgttg
    30421 cgccatgcga taacggcttc gttttgatct ctgaagacca ttccttcgcc ttcgcgtcct
    30481 tcttcttcca ttgtcaagta gtagttacct agaaccatat cttgagaagg agtaacaact
    30541 gatttaccat ctttagggtt caagatgttt tgagcagcta acatcaacat acgtgcttct
    30601 gcttgtgctt cttcattcaa tggaacgtgg acagccattt ggtctccatc gaaatcggca
    30661 ttataagctt cacatacaag tggatgcaaa cggattgcgc gtccttgtac taaaactggt
    30721 tcgaatgctt ggataccaag tcggtgaagc gtaggtgccc ggttcagcaa tactgggtgt
    30781 tccttgatta cgtcttctaa aacatcccat acttcgtctt cttgacgttc gattttacgt
    30841 ttcgcatttt tgatgtttga agctagttcg cgttgaacaa gctcacgcat cacgaatggt
    30901 ttgaataatt caatggccat ttcttttgga agtccacatt ggtacatctt caagaaaggt
    30961 ccaacaacga taacggaacg acctgagtag tcgacacgtt tacctagcaa gttttgacgg
    31021 aaacgacctt gtttcccttt caacatatga gaaagagatt tcaatgggcg gttacctggt
    31081 ccagtaactg gacgtccgcg acgaccgtta tcgatcaatg catctactgc ttcttgcagc
    31141 atacgttttt cattttgaac gatgattcca ggtgcattca agtctaacaa acgtttcaaa
    31201 cggttattac ggttgattac acgacggtaa agatcattca agtcacttgt tgcaaaacgt
    31261 ccaccttcta attgaaccat tgggcgtaaa tctggtggaa taacagggat aacgtccata
    31321 accatccagc ttggttgatt tcctgaagcg cggaatgctt ctaaaatatc caaacggcgg
    31381 attgcacgtg tacgtttttg tccttgtgca gatttcaatt cttctttcaa ttcggctgct
    31441 tctgcatcta gatcaacatt atcaagtaat tgtttaatcg cttctgcacc catagcagct
    31501 tggaattctt gtccatattg ttcacgtttt tcgcgatatt cgcgttctgt caacaattgt
    31561 tttttctcta aattcgtatt tcctggttcg atgactacat atgaagcaaa ataaatgatt
    31621 tcttccaaag cgcgtgggct catgtctaat acaagaccca tacgtgaagg aatcccttta
    31681 aagtaccaga tatgtgaaac aggagctgct aattcaatat gtcccatgcg ttcacggcga
    31741 actttcgaac gagtaacttc cactccgcat cgatcacaaa cgattccttt ataacggata
    31801 cgtttgtatt ttccgcaggc acattcccag tcttttgtag gaccaaaaat ccgctcacag
    31861 aacaaccctt cgcgttcagg ttttaaggta cgatagttaa tcgtctcagg tttttttact
    31921 tcaccgtaag accagcttct gattttttcg ggagaagcca aacctatttg catactttcg
    31981 aatttattta catcgatcaa aaggggttcc ctccatttca tttaatggca aaccgcaaga
    32041 cggctgatct tcgaccgcct tgcagctcat agaccggatt agtctaaatt atcttctgcg
    32101 gtttcaaagt tttggatgac atcgtctttt tcatcttcca cttgttcagc agctttcttt
    32161 tctaattctt tggcagtttg ctgttcggca aattttgtca aagcatcaac agtgatcaaa
    32221 tcgtcgtctt catcgtccat gtcgcgaagt tcgatctctg tttcttcgat atccaagaca
    32281 cgcatgtcca gacctagtga ttgcaattct ttcaccaata cgcggaatga ttccggtaca
    32341 cctggttttg gaattggttc acccttgacg attgcttcgt atgttttcac acgtccgact
    32401 acgtcatctg atttgtatgt caagatttct tgtaaagtat aagccgcacc gtaagcttcc
    32461 agtgcccata cttccatttc accaaaacgt tgtccaccaa attgagcttt acctcccaat
    32521 ggttgttgtg taaccaatga gtaaggtccg attgaacgtg catgcaattt gtcatcgacc
    32581 atgtgagcaa gtttgatcat gtacatcaca ccgacagaca cacgtccatc aaatggttca
    32641 cctgtacgtc cgtcgtacag aacagttttc gcatcgctgg ccataccggc ttcacgaact
    32701 gtttcccata cgtcttcatc acttgcacca tcaaatactg gcgttgctac gtggatacct
    32761 aactgacgtg cagccatacc caagtgtaat tccaatactt gcccgatgtt catacgtgaa
    32821 ggcaccccta aaggattcag catgatatcg attggtgttc catcaggtag gaatggcata
    32881 tcttcttccg gcataatacg ggatacaacc cctttatttc cgtgacgacc ggccatttta
    32941 tccccttcat ggattttacg tttttgaacg atataaacac gaactaacat gtttacgcct
    33001 ggtgacaatt catctccagc ttcacgagta aagattttca catcgtgaac gataccgccg
    33061 ccgccatgtg gtacacgtaa tgatgtatcg cggacttcac gagctttttc gccaaagatc
    33121 gcatgcaata gacgttcttc tgcagataat tctgttaccc cttttggcgt gactttccct
    33181 acaagcaaat cgccatcttg gacttctgca ccaatacgga tgatacccat ttcgtctaaa
    33241 tcttttaatg cgtcttcccc gacattaggg atttcgcgtg tgatttcttc aggtcctaat
    33301 tttgtatcgc gggcttctga ttcatattct tcaatatgga tagaagtgta tacgtcatct
    33361 tttaccaaac gacggctcat gatgatcgca tcttcatagt tgtacccttc ccaagtcatg
    33421 aaaccaacta aaacgttttg gcctagagcc atttcgcctt gttccataga tggtccgtct
    33481 gccagagtat cccctttttc gactttctca cccaaatgaa cgattggacg ttggttgtag
    33541 cttgttccgg agtttgaacg acggaatttt gttacatcgt atttatctaa tgcaccgttg
    33601 tcgcggcgta cgcggatttc agaagcatca acgtattcaa cgacaccgtc atgtttacat
    33661 aacaaagcag ctcctgagtc atgcgctgat ttgtattcca taccagtacc aacccatggt
    33721 gcttgaggat tgatcaacgg cactgcttga cgctgcatgt tggcacccat caaggcacgg
    33781 ttggagtcat cgttttccaa gaaaggaata catgctgtcg caacggcaac tacttgttta
    33841 ggtgatacgt ccatgtagtc tactttatca atagatactt ctaagttttc actttgagca
    33901 cgagccataa caacgtcttg agcaaatgtt ccatcttcat tcaatgggct gtttgcttgg
    33961 gctacgatgt aatggtcttc gatatcagct gtcaagtagt cgatttggtc agtaacacga
    34021 cctgtttcac ggtctacacg acgataaggt gtttcgataa atccgaattt atttactttc
    34081 gcataactgg acaaactatt gatcaacccg atgtttgggc cttcaggagt ttcgatcgga
    34141 cacatacggc catagtggga gtagtgaacg tcacgaactt cataaccggc gcggtcacga
    34201 gtcaatccgc caggtcctaa ggctgaaaga cgacgtttgt gtgtcaattc gccaagaggg
    34261 tttgtttgat ccatgaactg tgacaactga gaagaaccaa agaattcttt gatacttgcc
    34321 acaactggac ggatattgat caattgttga ggtgtcaatg tttcagtatc ttggatcgac
    34381 attctttcac gaaccacacg ttccatacgt gctagcccga tacggaattg gttttgtaaa
    34441 agttcaccga ctgaacggat acgacgattt cctaagtggt cgatatcatc gacattgccg
    34501 ataccttcca tcaagttcaa gaaatagctc attgaagcaa tgatgtcggc tggacgaacc
    34561 gtttttacag ctgcttctgg atagccgttt ccgatcacgt ttacttcacg ttcagggtct
    34621 cttggtgaaa atactttgat gacttggaca gtcattggat ctgtgaccac accgtcttct
    34681 gatggatagt aagtcactga atttaggcca ttatcaatga aaggagctag tgtttccatg
    34741 acttggtgag tcagaactgt tcctttttca acgatgatct cgcctgtttc tggatcaact
    34801 aatgtttcag ctaatgtcag attcaataga cgtgttttca aatctaattt tttgtttact
    34861 ttgtaacgac caacgtttgc taagtcgtaa cgttttggat cgaagaaacg agcattcagc
    34921 aagttacgtg agctgtctgc tgtttttggt tcgcctggac gcaaacgttc gtaaacgtct
    34981 ttcaaacctt cttctgttct tgagtcgctt gcgtttttat gcaagtcttt ttcgactgta
    35041 ttgcgtaatg tttcgctgtc gccgaagatt tcaaagatcg tatcatcgga accaaatcct
    35101 aacgcacgaa ctaatacggt taaaggaatc ttacgtgtac ggtcgatacg tacataagag
    35161 atgtcttttg catcagtttc catttctagc caagctccac ggttagggat aacagtagaa
    35221 ccgaatcctt cttttccgtt tttgtcgact tttccatgga aatagacacc tggtgaacgg
    35281 actaactgag aaacgattac tcgctctgcc ccgttgatga tgaatgttcc ttgttctgtc
    35341 attaatggga aatcaccgaa gaatacttct tgtgctttga tttcgccagt ctcgcggttt
    35401 gtcaaacgca atgttacatg taatggtgca gagtagtttg catcatgcgc acgagcttct
    35461 gctactgtat actttggttc tttcaattca taatctacaa attccaaaga tagattgcca
    35521 ttgaagtcat cgattggcaa aatgtcttca aacatttctc taagtccctc atctaagaac
    35581 cattggtaag agtctgtttg aatttcgatc aaatttggta attccaacac ttcactgatt
    35641 cttgcgaaac ttctacgttc gcgatgtttt ccgtatttta ctacgtgtcc agccaagctc
    35701 ttcacccctc taaaatgtca ttcaaaaaga ctacccgatc aattgttaca ataatatttc
    35761 aaatattatt tttaggtaac aacagacatt ttttgcggct tttaggcaaa aaaaaaccaa
    35821 aaatagaaca ttctcttgaa accttacttc gtgagcttct acttttgttt ccttatatta
    35881 aagccgaaac ggacaatatt tcgtttctgc cttttcttca tcagatagtt tttgcaactt
    35941 catatactac acttattttc tataaaagtc aacacttcct ggcgtttctt ctgctttttc
    36001 gcaacatttt cttaatgttt tcaattttat gttttcaatt atctgacaat tcttaattga
    36061 cagacctttt tatctcggtt atactttaga aaattcattt tgtggaggaa cttctatgcg
    36121 aaccaacatc aaccaaatcg caccaacaca actgaataac ttttacttta gctactttag
    36181 ataggtttgt attcatcatg atggatacaa acgttaaacg acagtttgta tctatgatgg
    36241 ctaaagaact ttgtattgca gcaaaaagcc acgtagacaa aatgctaagt ggctgagcag
    36301 tgaaaataca aagtaaaagt atcaagcatt ctactttttc aatttatttt ccgacttttc
    36361 aatccatttg gacattttgt ctgaatggat tttttgtttc ccaaaattaa ggaggaaaaa
    36421 gaaatgaaaa aaaatataat catcggatta ggcgctgccc tgctactagc tttaggaggt
    36481 tgcgcacaaa aagacaattc cgcagaggca tcagatggac aaaaaacaat tggcgtattg
    36541 caacttgtac aacataactc gttagatgct gcctataaag gattcaaaga aggattagca
    36601 gaagaagggt ataaagaagg cgataatcta acaatcgatt atcaaaatgc ccaaaacaat
    36661 caagacaacc tgaaaagtat gagtgagaaa ctcgtcaaag aagatcctga tttgctactt
    36721 ggaatcgcca ctccagcagc acaatcttta ttaaatgaaa caaccactat cccgatgact
    36781 gtcacagcgg tcacagactt ggaacaagcc catttagtca catccaatga aaaacctggc
    36841 ggcaatgtga ctggcacgac agatatggtg ccaatcgaaa aacaaatcgg cttactgtta
    36901 aatatcgtgc cagacgccaa aacaatcggc atcatgtata acaacggtga agcaaactct
    36961 aagatccaag ctgatttggc agaaaaagaa ttgaaaaaag cgggcgtcaa agtcaaagta
    37021 ctgacagcaa attccacaaa tgatgtccaa caagtgacga aaagtctagc taaagatgta
    37081 gatggaatct atattcctac tgataacacg ttcgcaagtg cggcaagtgt tgtcggtgaa
    37141 gtcgcaaaag aaacaaaaac acctgtcgtt gctggcgcag ttgatcaagt caaagacggc
    37201 ggtctagcaa cagtaggaat cgactacgaa agcttgggca aacaaacagg gatcatggca
    37261 gcaaaaatct tagacggcaa gaatccaagt gaacttcctg tcgaaacagc gaatgatttg
    37321 gaactgtatg tcaataaaga catggccaaa gcattaggca tcaatccaga cagcatcaaa
    37381 gaagctgaat aaaacacagg aggattctca tgacaaattt gctctctctt ttcttatcat
    37441 ctgcatcgca aggggtctta tgggccctac ttgcgattgg tgtatacgtc actttccgta
    37501 tcttggacat tgccgacttg actgctgaag gaagctttcc tttaggtgca gggatcgcgg
    37561 cagtaagtat tacaaatggc tatcatccat tgacagcttg tcttctaggc tttttagggg
    37621 gtgcggcagc agggcttgtt tccggactgc ttcacacaaa attgaaaatt cccgcccttc
    37681 ttgcagggat catcaccatg actggtttat attctgtcac atcccgtgtg atgggtgcgc
    37741 cgaacatctc tttattgggt cagccaacga tctttcattg ggtcgagtca ttcggcataa
    37801 caaaagacaa cgctgtactg atcgtcggtc tgctttttgc tttgatcgtt gtatgtttac
    37861 ttgttttgtt cttacgtaca gaaacaggac ttgctattcg tgcaactggc gataatctgg
    37921 caatgagcga agcaaacggg atcaacacag ataatatgaa gatcatcggt tatatgattt
    37981 ctaatggatg cattgctctt tctggctcct tgcttgcgca aaacaacggc tttgctgatt
    38041 taaattctgg tattggaacg atcgtaattg gacttgcttc tattatcatc gcagaagttt
    38101 tgttacgtaa ccagccacta tttctccgtc tcttgactgt cgttttaggt gcggtcattt
    38161 atcgttttat tttagcgtta gtcttcgaac tgaatgttga accaagtgat tcgaaacttg
    38221 cttctgcttt cgtgctagtg atctgtcttt ctttcccgca gatccagcaa aaattccatt
    38281 tacctcaaaa aaagaaaggc ggtagtgtca aatgaatgac gtcttaacat tggaaaatct
    38341 ccatcatacc tttgaaaaag gaacgatcaa cgaaaatcat gtcctgaaag gcattaatct
    38401 ttccttagat cccggcgact tcgtgacgat catcggtgga aatggggctg gtaaatctac
    38461 tttgctgaac agcatcgccg gtacattttc tgtcgatcag ggtcaaattt tattgaatgg
    38521 aaaaaatatc acgaaaaaat ctgttgtcga acgttcgaaa tccatcagtc gcgtattcca
    38581 agatcctaaa ctagggacag ccgttcgttt aacggtagaa gaaaatcttg ctcttgctat
    38641 gaaacgagga aaaaaacgcg gcttcttccg tggtgtcaaa ccacaagacc gaacattttt
    38701 caaagaccag ctggttcggc taaatttggg gttggaaaat cgtttatcct cagaaatcgg
    38761 tttgctgtct ggaggacaac gtcaagctat tacgttattg atggccacat tacagcgtcc
    38821 agaactgatc ttattagatg aacacactgc agcgcttgac cctgctactt ccaaaacggt
    38881 gatggaattg acagaaaagc tgatcactga gcagaatctc actgctttta tggtcactca
    38941 tgacatggaa gacgcgatcc gctatgggag tcgactgatc atgctgcatc aaggaaggat
    39001 cgtcgtagat atccagcaag aggaaaaaca gcagctcaca gtgccccaat tattagcgtt
    39061 gttcaaacaa aacagcggca cggcattaaa agacgatcaa gtacttttat cttcataatc
    39121 agaaagagga tgggac
//
LOCUS       DAFBQH010000019        39076 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.019, whole genome shotgun sequence.
ACCESSION   DAFBQH010000019 DAFBQH010000000
VERSION     DAFBQH010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.019"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            6..305
                     /locus_tag="LK694_001038"
     CDS             6..305
                     /locus_tag="LK694_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5051925.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="LK694_001039"
     CDS             378..1739
                     /locus_tag="LK694_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBK5051926.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="LK694_001040"
     CDS             1761..2021
                     /locus_tag="LK694_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051927.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="LK694_001041"
     CDS             2035..2787
                     /locus_tag="LK694_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBK5051928.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="LK694_001042"
     CDS             complement(2881..3486)
                     /locus_tag="LK694_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK5051929.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="LK694_001043"
     CDS             complement(3539..4513)
                     /locus_tag="LK694_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBK5051930.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="LK694_001044"
     CDS             complement(4891..5610)
                     /locus_tag="LK694_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5051931.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LK694_001045"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LK694_001045"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBK5051932.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="LK694_001046"
     CDS             7161..8558
                     /locus_tag="LK694_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK5051933.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="LK694_001047"
     CDS             8639..9079
                     /locus_tag="LK694_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK5051934.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="LK694_001048"
     CDS             9227..9553
                     /locus_tag="LK694_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK5051935.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="LK694_001049"
     CDS             9554..10303
                     /locus_tag="LK694_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051936.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="LK694_001050"
     CDS             complement(10383..10838)
                     /locus_tag="LK694_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBK5051937.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="LK694_001051"
     CDS             complement(11265..11540)
                     /locus_tag="LK694_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051938.1"
                     /translation="MKLEDKKVILKKIKRLPEQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="LK694_001052"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="LK694_001052"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBK5051939.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="LK694_001053"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="LK694_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBK5051940.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="LK694_001054"
     CDS             14770..17436
                     /locus_tag="LK694_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK5051941.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="LK694_001055"
     CDS             17800..18552
                     /locus_tag="LK694_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK5051942.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="LK694_001056"
     CDS             complement(18574..19728)
                     /locus_tag="LK694_001056"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBK5051943.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="LK694_001057"
     CDS             19913..22354
                     /locus_tag="LK694_001057"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBK5051944.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(22684..23388)
                     /locus_tag="LK694_001058"
     CDS             complement(22684..23388)
                     /locus_tag="LK694_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051945.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(23575..23820)
                     /locus_tag="LK694_001059"
     CDS             complement(23575..23820)
                     /locus_tag="LK694_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051946.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(23837..24688)
                     /locus_tag="LK694_001060"
     CDS             complement(23837..24688)
                     /locus_tag="LK694_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK5051947.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            24854..25858
                     /locus_tag="LK694_001061"
     CDS             24854..25858
                     /locus_tag="LK694_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBK5051948.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(26099..26455)
                     /locus_tag="LK694_001062"
     CDS             complement(26099..26455)
                     /locus_tag="LK694_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK5051949.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            26823..28010
                     /gene="tuf"
                     /locus_tag="LK694_001063"
     CDS             26823..28010
                     /gene="tuf"
                     /locus_tag="LK694_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBK5051950.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(28198..30789)
                     /gene="mprF"
                     /locus_tag="LK694_001064"
     CDS             complement(28198..30789)
                     /gene="mprF"
                     /locus_tag="LK694_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBK5051951.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(30815..31576)
                     /locus_tag="LK694_001065"
     CDS             complement(30815..31576)
                     /locus_tag="LK694_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK5051952.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            31854..32375
                     /locus_tag="LK694_001066"
     CDS             31854..32375
                     /locus_tag="LK694_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HBK5051953.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            32434..33294
                     /locus_tag="LK694_001067"
     CDS             32434..33294
                     /locus_tag="LK694_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBK5051954.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            33479..35794
                     /locus_tag="LK694_001068"
     CDS             33479..35794
                     /locus_tag="LK694_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK5051955.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            35976..37817
                     /locus_tag="LK694_001069"
     CDS             35976..37817
                     /locus_tag="LK694_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBK5051956.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            37841..38950
                     /gene="rpoD"
                     /locus_tag="LK694_001070"
     CDS             37841..38950
                     /gene="rpoD"
                     /locus_tag="LK694_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBK5051957.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT        12572 a         6875 c         8095 g        11534 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttgctct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttgctt ttttctctat cagaaaatat
    22441 gtttattatt agaatttttt atagttaagg ttaggcatct taagaaatca ggcaactcca
    22501 agatatcttc aggtagaaga gaagtcgcct aacattgtag gatcataggt gatttgttgg
    22561 acaggtcaag ctgatatttg agcctgatgt tagaaggaat ttctttttac ttgctgtaga
    22621 aattattata tgactaaaaa ggctgggatt ttaaatccca gccttttatg acaaatagaa
    22681 tttttatcga tcagtaatcg ctgtcgctgc aaattgttcg atcaaaggaa tcaaagtcaa
    22741 tttgactttg tatttccgtt cgagtgcttt ttcgataacg aactcatttt cagtagtcat
    22801 agaaatagtt gctaatttac gaggttcatc agcatttttt tcattaataa ctacttcatg
    22861 ttcattttgt tctgtgtaaa atttaggata ctcagcaacg gcttttgaaa aatcagcaaa
    22921 acttaaagcg gcattcgtat cagttgagcg tgtattttca gttgtgtccg cttttggttt
    22981 ttccgttttt tcttctggca cactgactgt ttcttctgta actggtgtcg caacagcttg
    23041 cgcagttggt tcatcgtttt tcaatcgttg atcattccag taagacacga tttcaataag
    23101 aagattgtca cggaatcgtt cgaaaattcc gttttctcct gaacgaattc tctttaatac
    23161 agtattgaag ttgtcatcga ttggcacgtt gatccgtgta tcttcatttg ttttcccagc
    23221 taatccgatc gttaagattt gacggttttt gggatcaagc atgacttgta acggcaaacc
    23281 ataacgtcgc aagctctctt cttctagaga acggaataat tgtcctctta caccatccaa
    23341 tgaaggacgg aattgattta ataatttttg tttctgtttt ttcttcatat gttctctcct
    23401 tcgctcatac cctttcgatt ataagaaaaa aacagccaaa tgaaaaggag taatgcaaaa
    23461 aaacttgcgt atacgcaaat tacaacagaa aaatttgcgt attttcccgt tttttgaaag
    23521 aaaaataaaa aatggaacgg attatgtata tatacataat ccgttccatt ctttctattt
    23581 tttcggtatc aaggcaaaaa caggtagttc aggtgaataa atgacttcat gttcgtgcca
    23641 gggtttgacc attagcgaat gatcgaaccg ttttttcagt tcttcgatga tttgatcatg
    23701 tgtccagttt cttgcaggaa aatgttggat tttctctgca tcgatcaaaa agacaaaatc
    23761 ggaatgaaaa gtctgggtaa cttcttccga gagagaattc aagcgttcga ttggatccat
    23821 aaaatcacct ttcgacttaa aattgggtag tatctgggtc ttttgctttg ccgactacac
    23881 catctaactg atcgatttgt ttcatttctt cttctgttaa ttcaaaatca aataattcag
    23941 tattttcttt gattctgctt ggtgtcactg acttaggaag aggaaggaat tcgtgttgca
    24001 gcgaccaacg caaagcaact tgtgcaatcg tcttgtcatg tgcttcagcg atttgtttca
    24061 tttcaggcac atcgaagatt ttaccagtgc ctaatggact atatgcttcg aggatcatat
    24121 catgtttttt tgcatactca actacagttg tctgtaattc gcctggtgca aggaaaactt
    24181 gattgaccat cggcataatt tttgctgttt ttgctagtgt atctagatgg tggggtaaga
    24241 aattgctcac accgattgct ttgattttac ctgcttcata cagttcttca aatgctcgcc
    24301 atgtatctgc atttgcttgt tcccaattat cacgaaaagc aacagggttt ggccagtgga
    24361 tcaaaaacag atctaaataa tctgtttgga gcttcttcaa tgattcttca aaagaagaca
    24421 tcacaagttc atagctgtgg tttgcattcc ataattttgt tgtcaagaat atctcttctc
    24481 taggaattcc actttcctta atcgcttggc cgacgctttc ttcattcttg tatccttggg
    24541 cagtatcgat atggcgataa cctgcagcca gcgcttcttt aacagaggaa acagcgacat
    24601 ccccatcagg tgtttgccat gtgccgaatc ctactaccgg gatttcgtaa ccatttgata
    24661 gacgataagt gcttgttaat gaattcatac tttcagctcc ttttttattc gttcaactat
    24721 aagtgtagcg catttttcga aaaaaagaat ccattttgtt tctactcttg aaatttttgt
    24781 ttaaaagaaa atgattttga ttctggtatt tttgattttt tttccgtaaa ctaagacaga
    24841 agggaatgag aaaatggata tctatttaaa aaaagcaatt ctgcatatta ttgacagaga
    24901 aactggttct ccagtctttt cacaaaaaga gctggatctg acaaaagaat atattcgtga
    24961 ttttcttcaa aagaagatcc aaaaaatctc ttctgctcaa acaaaaactg gacaactagc
    25021 agaagaaagc atgatcgttg aatcacttaa aatcgctcaa gaaaattttg tggaagcaag
    25081 tgaagcaatc gttcagcggt ggttcgatat ctaccaagaa agtgaagaag cacctagtgc
    25141 agatgtattt gttgtcttgt atgaattaga tacagtgatg tacttatctt tattgaaagt
    25201 caattatcgt gaggcttaca ctcactttgt tgaagcagac gaagcaggga tcgacaaccg
    25261 cttgattttg aatcgagcaa ttctaggggc gaaaacacaa aaagcagatg aagcattcgc
    25321 tgtgaatttg actgattgga cctacgaatt aattgaaaaa aaatatgagt tttctggcaa
    25381 aaaagagctt tatttttctg gaagagtgat cgaaagtgtt cctgcacctt ctttagaaga
    25441 aaatgtaaaa gtgatccaaa aagtagccaa gcatcttgga aaaaaatttg agacagaaga
    25501 atttgatatc atggctgatt tgaaagaagc tgtctatgat acgatagaag aaaaaggtcg
    25561 gttggatcat gagatgattg cggaaaaagt cttcaaggaa aacatcacgg caaaaatggc
    25621 ttttaaagaa gaagtacaag agcaaggatt tgtcccagaa gcacctcctg taaaagaagt
    25681 acaagaaatt tctgagaaaa aatttggaaa acaaaaattc aagctatcaa acgggatcga
    25741 actgatcgta ccagtagatg tatatcgcaa cccagatttg attgaatttg tgaataatcc
    25801 ggatggtacg atatccgtga tgatcaaaaa tgtggatgaa gtattgaatc gtttataaaa
    25861 atgactagaa tattaggtgt tcaagtacca gtgatcagtt aaatgaattg tgtcaagaga
    25921 aggatcggaa aagacgaata taacaaaaaa agagcgtgag acaaaagtaa agaacacttt
    25981 tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt cttatttctt
    26041 ggagttaaac gcttctgtcc cagcctcttt ttatctaata aaattttaag accatttact
    26101 aattatcgat tttttctgcc gcatttttag gcacttcaga agcctttatt tcttcccagc
    26161 ttaatacacc tttacgagga ttgactttga gtttaagata cgcattcatc tttaatggat
    26221 gttctctttc ttcgcgaagc gtcacctgtt ttttttcacc atctttatta taagccgctt
    26281 gcttgtaggt ataagtcgtg aatttctctc cgttatctgc ttgggtagct gacatttcgc
    26341 cattcgtcgt gatttttgta taataagctt cgccgccgta aaaataatga taagctgcaa
    26401 aacttccgcc gagaaatatg cctaaaacga caagtacgcc aattaatttt ttcatgtact
    26461 tcacctcttt ccttattatt aacgaaatag aagatttcgt ctattgaaag ttgttggcaa
    26521 cagctgaatt ttaaactttt cctcattttg ttcttattgc ttcaaaagaa agttaaggac
    26581 tatcttgaag tttttctcaa aaaacacatt tttttattta taaaaggtac ttttttcgcg
    26641 tataagacag acgagtgctc ttagttgact ttgaccaata ttgacgtata ataagcttag
    26701 ttcgaatctt acaaaaagta agtcggacgt attctttttg atttagtcaa aaaaagacta
    26761 taataaaaag gtgagagctg aaatttttca gtgatctata ttatttagga ggtttgtaaa
    26821 ttatggcaaa agaacattat gacagaagta aaccacacgt taacattggt acgattggac
    26881 acgttgacca tgggaaaact actttaactg ctgcgatcac tacagtatta ggcaaaaaag
    26941 gcctagcaaa tcctcaagac tatgctagta ttgatgcagc tccagaagaa cgtgaacgtg
    27001 ggattacgat caataccgca cacgtagaat atgaaacaga aaaacgccac tacgcgcata
    27061 ttgatgcacc aggacacgcg gactatgtga aaaacatgat tacaggggca gcacaaatgg
    27121 atggtgcaat cttagttgtt tctgcaactg acggtccgat gcctcaaaca cgtgaacaca
    27181 ttttattgtc acgccaagtt ggtgtaaaat acctgattgt tttcttgaac aaagttgatt
    27241 tagtcgatga tgaagaattg atcgatttgg tagaaatgga agttcgcgag ttattgagcg
    27301 aatatggttt cccaggcgat gacactcctg tgatcaaagg ttccgcatta aaagcattgc
    27361 aaggcgatcc agatgctgaa gctgctatta tggaattgat ggatacagta gatgaatata
    27421 tcccaacacc agaacgtgat acagataaac cattactatt gccagtggaa gacgtcttct
    27481 caattacagg tcgaggaact gttgcctcag gtcgtattga tcgtggtgct gttcgtgtcg
    27541 gtgatgaggt agagatcgta gggatcaaac ctgaaacaca aaaagcagtt gtaacaggtg
    27601 tagaaatgtt ccgtaaaacg ttagattacg gggaagctgg ggataacgta ggcgtgttgt
    27661 tacgggggat ccaacgtgac gatatcgaac gtggacaagt acttgctaaa ccaggttcca
    27721 ttactccaca tacaaaattc aaagcagaag tgtacgtgtt gacaaaagaa gaaggtggac
    27781 gtcatactcc attcttcaac aactatcgtc cacagttcta cttccgcaca actgatgtta
    27841 caggaacaat cacattgcca gaagatacag aaatggtcat gcctggtgac aacgtgacga
    27901 ttgatgttga cttgatccat ccaatcgctg ttgaaaacgg aacgacattc tcaattcgtg
    27961 aaggcggacg gacagtaggt tcaggtatcg ttacagagat cgaagcataa gaaacgcgac
    28021 tcgaatattt tggtaatgaa aaatgtttaa aataaaagag cggtccaaac aggatttctg
    28081 tttggaccgc tctttgttct tcaattgtat aagaagagat tgtagaagca tgaactttcg
    28141 ttttacttca gtctctttta ttcatcatga gagtgttcat ctatcggtat gcagttttta
    28201 atactttctt cgtatccatt ttttcaaacc acttattcta ccttgtgtct gctcaactgg
    28261 cgcattatca ataatcagca aagcaatcat aacatatgca atccaactat ctctagaata
    28321 tagagtataa cgtgagaccc agtttgtagc gtacttgctt ttatattctc ttaatccatg
    28381 gaaagaataa aaatgagaac caaactcgta aaccaagtat gcaatccttt cctgtaaaaa
    28441 actctttctg gaagttccga catttgaaag aggggccatc cctaaattga accattggat
    28501 atgttcttct ttcatatagg caaacaaatg caagaacaag aaatccatac tgccagaagg
    28561 tgctttttta gaatcatatc gcatcagatc aatcgttcct actttattgt tcgtgtaagt
    28621 aggcatgatc gttgcaaaag cgacaatttc ttcttgggta tttttgacaa ctgcaatagg
    28681 cgctctttgt agataatctt cagagaaaaa gcctaaagaa aacccttttt cctttcgtga
    28741 acccaaccaa ttatcagaga tatttttgaa agtactcatt tgctcagtag aaaatggtgg
    28801 ttgtaagaca tcaaatgtaa atccttctcg ttcgatccga ttcaagacag cacgagtgcc
    28861 cttcattttt ttccctgaag ttgtaaaact attcaggtct acataagctt cttcccccat
    28921 cttaatgaaa tcgtagccga attcatgcaa aatcataaca atctcttcgc ttgtttcgta
    28981 gaaaactggt aaatagcata gtcgatctgt ttcttcgata aaagcttcga ttgcttctgg
    29041 aaaatcttct ttcttaccag aaggatcacc cattacaata catttattat tgaagcaagc
    29101 gaattggaga agaacagttg gttcaccgtc tttttcatag gcaaacatcc gcttatcttt
    29161 taagaagatc aattgactat cggagtttcc tccataagtt gtaaggatct tcagggcctt
    29221 ttcttcatta aaggcttcgc caatctgttt cttttctcct tgaaggaaat gaacaaatag
    29281 aacgatcatg aaactaacag cgatgatggc caacaatccc gaaaaccaaa tcttttcaga
    29341 aggaaataag aaaaaggaaa taaaatggtg acgccgatga gggaaatccg gcagattgta
    29401 taccccaata acgatgtaaa gcaaggacag tgcaccaata atgattccat cgatcgtacg
    29461 ccactcccat gaatagatca attgttcccg aaataattcg tttttagaag caagaatgat
    29521 cagaagcaga atagacaaga aaaccaccgg tgtgaaacta aaatcactta ataaaacata
    29581 aagtaaagct agaacaatca agaagattgt aggcaaatat gctcgtttca cacgtgctgc
    29641 gatccctctg cccataatga ttaaaagaaa tcccaatagt atactaggaa attggataat
    29701 aaaatgaaat ttcaatgggt tcaaactatg aagccagcga aactctgtaa aagcttgcgg
    29761 gatagtggca gaaagtacta gcatgattcc agaaaagtat aataagacaa ccacgatttt
    29821 atgcgcaatt tctgttgcta attgtttagg gataccagaa taacgctgat caaaagtgga
    29881 accgatattt ttgaagaaaa agacgattcc aatcaaaaac ggaatgatgt agtagaacag
    29941 gcgatagagc aagatccaca caacgactat ttctcttggc acaccaatag cggacaaccc
    30001 gataatcatc attacatcga aactcccaat ttctcctgga atcatagaca cgattcctat
    30061 cactgaagca gcaatgaata atggaattgt ttgccacaga tcaatccgga tttccattaa
    30121 ataaccaaca gaaaggaaac taaacaggac accggtccat tccaacaatg aaactagcaa
    30181 tagctgtcct tgagttttaa gtgaggtacc accaaggaat cctttctttt tgaaagtagt
    30241 aaataaaaag acgacaggaa aatatagacc gccgccgatc aaccatatcc aatattgctt
    30301 caaaaaagga gcaactggcg tgaagactac aagtaaaaaa gaaagcagac tgtaaataga
    30361 aagtccagcc atcaaaaaca agaaaacttt cgataatgct tggatcacgt ttctgctctc
    30421 tttcttttgt ccatatagtt cagagcgaag ccccacacta acaaacccgc cgaaaccggc
    30481 aatattgttg atcgtattga tcagccagct tgtctcaaac aggtaacgag gattctgctt
    30541 ttgttcaagc atccgattca aaataatatc ataaccaacc ataggtaaca cagagataag
    30601 accaatcaat agcattagtc cgcttttcca tacaggtata gtggcaaacg tttctcctaa
    30661 ttgctggata gatagagttt taccgatgga catcaattca ccgacaataa tgacaatgac
    30721 agaaatcaaa aagattgttt tgaacagtcc taggtggttt ttcagccatt gtatggtatt
    30781 ttttaacaaa taaatcacct tctattccta atagttatgt ttcagaaaga aattgaagaa
    30841 cttctcgatt gaagcgttca ggatcttttc tagctaattc atgtccttgt tctttcacaa
    30901 gcacaaagga agcttttggt atagttttag ctatatacaa agaatgtttt agcttgatta
    30961 catctttttt tcccacgatg atcaaagtgg gggagttgat tttttttaaa tcattttctg
    31021 taagcccgat gtcatgcagc aaaagtttga taactaaaag atttttgcga agacttgggc
    31081 gaaataaaga gagtaaccat acccatgcat aatgaatatt actgatgact ctggcagaaa
    31141 aacgaacgcc tttaacaagc gtgtttccag aatttaaaat caatcgatga acttttttag
    31201 ggaagctgct ggcaaatacc agtgctagat ttgctccatc actaaatcca agaaaatctg
    31261 cctgatcgat tttttccagc aacatgatcg tattcagatc ctcggccatt aactgaaaat
    31321 tcaacataga tgcttcgttt gtcgaacgac catgtcctcg gctatctact agatacacgg
    31381 tatagtagcg ttcgagtaca gggacctgtt ctgaaaaaaa gcggccgctg ccgtcattgc
    31441 catgcagtaa aaagagaggg aagccttgac cgctcttttc atagtaaatt tcgctcccat
    31501 ctggcatttt tgcatatttc ttttcttttt tcacataatc acctactgtg taaagtataa
    31561 cgattatatc agtcattggg caattgtttt tactgaaaaa caacgatcat tttcgtaaga
    31621 tgtcaaaaaa tacaggaaaa ttcatttttt cttaaggcgt acacagaaat caaggaagaa
    31681 atagccaaga agataaagaa atagtaagat aaaaaaagat gtcaaaataa ctaggtgatg
    31741 aaatgaagcg aaaacgatta tctattatcc gtattctttt ttacatgatc attctgacgg
    31801 ttattggtgc ggtgttgttt ttatgaggaa gaacttatcg ttcggtaaaa cgagtggaaa
    31861 gctatcaaat agaaataaca acagctgcaa aaaattatca tatggaaaac tacgagaatt
    31921 tgatcaaggc gataatattg accgaatcaa aaggaaaagg gacagatctc atgcagagct
    31981 cggaaagtct gcatggagaa gctgagatca ttgataatcc agctgacagt attgatcaag
    32041 gtgtcaaata tttggcggaa atgatagtag aagcaaagaa acaaggatgc gatttatgga
    32101 cagctgtcca agcatataat tttgggaaag attatattgc ctatgtaaaa aatcatggcg
    32161 gagaaaacac agtagaatta gcagaagcgt attctaaaga tgtgctgagc ccacttttag
    32221 gaaatgagga agggaaacaa taccgatatt ggcggatcca gtccttgctt tacaatggcg
    32281 gctatctcta tcataatgga ggtaacatgt tttatcctga tattgtcaaa atgaatgaaa
    32341 aaataataga gacctatgaa aaagtcttta agtaaaatag caggaaatgt tcaatcatga
    32401 ataaaattaa atgaaagaag gagtgcgcaa atagtggaaa aaaggcaaaa aatggaaaat
    32461 caagaagtgg ggtacacaac caaagactgg atgttacggt ttgtgaaggg aatgtttatc
    32521 ggttcaggat ttatcttgcc aggagtcagc ggtggtgcat tagcagctgt ctttggcatg
    32581 tatgagcggc tgatttcttt tttagcccac cttacaaaaa atttcaaaga aaatgttttg
    32641 ttttttctgc ctgttggtct aggcggagtg acaggcatct ttctactttc ttttttagtc
    32701 agttttttat taggtgcgta tgaaacaacg atattatggt tttttgtcgg atgtatcgct
    32761 ggaactgttc ctgcgttatg gaaagaagca gggaaaaaag gaagagatac ttctgatgtg
    32821 atcatcatgg ttgttacttt tgttctggct tatttgtttt tattatacgg cgctcaatta
    32881 tttgacgggc aagtcgatca gaattttgga acatggctga ttgccggcgg attgattggt
    32941 ttgggtatga tcgtcccagg attgagccct tctaattttt tggtgtatat gggattgtat
    33001 aaagcgatgg ctgacggatt taaagaagta cgacttgaag tgattgtgcc gatcggaatt
    33061 ggtgggattc tttgtgttct tggattgtca aaaatcatgg attacatttt ctcaaaggca
    33121 tatagcaagc tattccattt cattttaggt gtagtttttg cttcaacgat tatgatcatt
    33181 cctaccgatt acgcgggatt gggagttgct ggggtactag cttgtcctgt tctttttata
    33241 gccggtacag cactaggttg gtggatgagc cgtttggagg aacaatataa gtaaagatag
    33301 attgataata aaaacagcac acgggtgatt caaacaaaaa ggttggcatc ttttttgtaa
    33361 aatcattcgt gtgtttttct ataaatttta taaaaacaag ttgtttttaa tcttggaaca
    33421 cgctagactt aaacacgtga ttatttttga aagttggcgg aaaatggaga aaaagaaaat
    33481 gaatggactt acacagcaag aggtagttca gaaaatggct tctggccagc aaaatgacta
    33541 ccaggaagat gcatcaaaaa gtacaaaaca aatattcagc gataatatat tgacgttatt
    33601 caattttctg aactttggta ttgggatttg tctgttattt gtaggtgcgt attccaacat
    33661 ggcgtattta gcaattattg tcgtcaatat cgttattgga atttatcaag aaatacatgc
    33721 aagaaatctt gtacggaaat tatcgattgt ttcccaatca gaagtactag tgatccggga
    33781 cggtaaagaa caatcgattt ctactaaaga gctggtatta ggagatttgg taaaaatcgg
    33841 agcaggagaa cagataccag ccgatttaca ggttatatct ggacgggcag aagcaaatga
    33901 agctcttttg acaggcgaat ctgatttgat tgtcaaagaa actggagctg aattgctttc
    33961 tggaagcttt ttgacaagcg gttctgtcat ggctgaagtt atccgagtag gtgcagataa
    34021 ttatgctgtc cgcttgacta gcgaggcaaa gactcacaaa gctattcgtt ctgaacttgt
    34081 agattcgatt cgcaaagtat ccaaattcac tagtttcgtg attattcctt tggggatcat
    34141 actatttttg gaagcactgt tcatccgcga tgcttctatc aaaatttctg ttattgcatc
    34201 tgcagcagct ttgttaggca tgcttccaaa gggtttggta ttgctaataa gtattgcatt
    34261 aacgacaggg gtaaccaaac tggcaaaaaa acgaatcctt gttcaagaca tgtattcaat
    34321 tgaaacgctg gcccatgtcg atacattatg cttggataaa acagggacca ttactgaagg
    34381 gaaaatgagt atccagcggg tagaagcact acatccgctg tacgagaaag ccatcccaga
    34441 aattatgggg acgtatctgg cagaaagcag tgataacaac atcactatgc gggctatgag
    34501 agattatttt gagctttcta atcggtatga tgtgacaaac aaagtcgcat tttcttctga
    34561 tcgcaaatgg ggcgcgatgg aatttccaga actgggagtc gtgtacgtag gagcgcctga
    34621 acgtttggtg gaagaagatc ggcttccgga cagtctaaaa acagcccaag cgaatggcta
    34681 tcgcgtactt atgttgggga tcgctccaaa tcagcaattg aacgaagaaa aaccagcatt
    34741 tgttgagccg gttgctctgt ttgaaatcga tgatccaatc cgaaaaaacg ctcaagaaac
    34801 actagcttat cttcatgagg aaggtgtgga tctgaaagtg atttctggag acaatccttt
    34861 tactgtctcc aatatcgcta gacgtgcagg attaccgaat tatgatgcgt atgtcgatct
    34921 ttctcaaatt actgaagaaa tggaagtgcg agaagctgct catcgctata cggtttttgg
    34981 acgagtgagt ccgcagcaaa agaaattgct ggtaaatgaa ttgaaagaaa gcggcagaac
    35041 ggttgcaatg acaggcgacg gtgtgaatga tgttttggcg ttaagagaag cagattgcag
    35101 cattgctatg gcagaaggtg atggagcaac acgtcagatt gcgaatctgg tattgttgga
    35161 ctctgatttt actacattgc cagaagtcct gtttgaagga cgccgagtgg tcaacaatgt
    35221 gacgaaagtc tcaggcatct tttttatcaa aacgatttat tcatttattt tatcgattat
    35281 atgtgccgtt acagcaattg gatttccgtt tatccctatc caaatcacgc tgattgattt
    35341 agcgattgaa gggtatcctt ccttcttttt atcttttgaa caagataaaa gaaaaatcac
    35401 ttaccgttat ttgcctacag cacttattaa tgctttacca aatgccttgc ttgtagtagt
    35461 gaatatcatt gcggtgtatc tacttggaaa aacattcgca tggggaaatg ctgaaactac
    35521 tactttgatg tattacttgc tgattgggat cagttgcatg gcagttgtaa aagcttgtta
    35581 tcccttcaac ccattgcgca tcttcctagc agtaacgacg atcgtaggga tatatgtagc
    35641 tgcaatgctg ttccatggaa ttttggaagt aaacttaggg ttgggagaga gcgtaggata
    35701 cttctttgta ttcatgctcg taaatattgt tcttcgagta ttgtactgga aggctgatat
    35761 tcaaaatcag ttactgaaaa gagtagcaaa ttgatgctta atgagataga aatgtgttat
    35821 acttgaaaaa gcaatgaaat atgatacaat caaacatgta taattgtgtc ctttaaaatc
    35881 gcactgattt cagttagtgc gattttttac cggttattct cctgaaatat cttctgttaa
    35941 acccaaaaga tatcaataga gcagggggtg aaaaaatggc tcaacggatt cctcaagagg
    36001 ttatcgaaac agtacgcagt caaacgaata tcgttgaggt gattggacaa tatgtccagt
    36061 tgaaaaagtc tggtaaaaat tacttggggc tgtgtccttt tcatgaagaa cgtacaccgt
    36121 ctttttctgt tgcagaagac aagcagattt ttcattgctt tggttgtggt aaaggcggaa
    36181 acgtgtttac ttttcttcaa gaactagaag gtctaagttt tccagaagca gtggtaaaag
    36241 tggcagactt cgaacagata ccgttagaag atcaatggcg tcagcgtact atagctgtcc
    36301 aaaatcctga atctgctacg gggaaactga ttgctgctca tgaaaaagca gcggaaatct
    36361 atcatcacat gctattgcat acaaaagctg gacagccagc tttagaatat ttgcaaagtc
    36421 gtgggttgtc tttagaatta attgaagaat tcaacatagg ttttgcacct aatgagcgtt
    36481 cttttcttca gcaagtattc aagaatgaat caatttcaaa tgaattgtta gaacgctctg
    36541 gattgtttgt cacgcatgat gatggccgta tgtctgatcg tttttatcaa agagtgatgt
    36601 ttccacttcg taattctcaa ggccagacga tcggtttttc aggacgctgg cttgcacctg
    36661 aaaaagggca ggaaaaagat cagcccaaat acttgaatag tccagaaaca gaactgttca
    36721 ataaaagaca agttttattt aatcttgata aagcaagaag cgatatccgg aaaaacggag
    36781 aaattttgtt gttcgaaggg tttatggatg tgattgctgc atggcaggct ggtatcaaaa
    36841 ctggattagc ttctatgggg acaagtctaa cagctcaaca gatccaacaa atggaaaagc
    36901 tgacgaaaga attggtcttt tgctatgacg gagacaacgc aggttttgaa gcaaccaatc
    36961 gagggattga attgcttcgg caaaacagtc gattagcact ttctgtcgta gtcgttccag
    37021 aaaagcttga tcctgatgaa tatctccgta agtatggaga agcatctttc caagaattag
    37081 tggctcatgg gcgcgaaaca gtctttactt ttaaatcacg ttatttaaaa caagggaaaa
    37141 atttagataa tgaaaaagac aagatcgaat acctcgaaga attactagtt gaacttagcc
    37201 gagttgtttc accaatcgaa caagatatgt atctatctca actgtcaaca gagtttcaga
    37261 tcggcagaga gacgatacaa aaacaactgc gcgatcttcg aaggacaaat cagcaaaatc
    37321 gtcaagaacc tcaggaaagt caacacactc aggttacgag acaagaaact gcacggaaaa
    37381 agagaccttt gacgcaagta gaaaaggcag agcagattct attatatcgt gcatttaaag
    37441 aagtttctgt ccgtaatatt ttaaaagaac gagaatttca gtttattcat gatgcatatg
    37501 cagaagttta ttttttattc gatgcatatg tctcacaaca ttatgaattc aaccttgcag
    37561 agtttttaga ctttttgcaa gatgaaaatt taaggcggtt ggtggtggaa atcgaatatt
    37621 taccggtggc agaagaaagt tcgccacgag aaatccaaga cttgctagat gtgatcagca
    37681 aatctggctt agctgatgaa ataacattaa aaaaacaaat gcagcaagaa gccagacgaa
    37741 ctggaaacaa acagcttgag cttgaattga cgattgaaat catcaattta acgaaacagt
    37801 taaaacaagc caaatagcta ctcaaggggg gccttctttt atggcaaatg gaacagataa
    37861 aaaaacgtat gaagcagcag tagcagcttt catcaaagaa aacaaaccca aacgacaagt
    37921 tgtatatgat gatttatcca atcagcttgc tacaccattc actttgaatg cagatgaaat
    37981 ggacaaattg atccaaaaga ttgaagacgc aggtatcagt gtggtggatg aaaatggtga
    38041 tccatcaatt cacagcttga agagtgctga gaaaaaagct gaacaagcaa aaacagaaga
    38101 tctttctgcg ccaaccggtg taaaaatcaa tgacccggtt cgtatgtatc tgaaagaaat
    38161 tggccgtgtc tcccttttaa ctgcggaaga agaagtagcg ttagcattga aaatcgaaga
    38221 aggcgaccaa gaagcgaaac agcgtctggc agaagcaaac cttcgtttag tagtaagtat
    38281 cgctaaacgt tatgtaggac gaggcatgca gttccttgac ttgatccaag aaggaaacat
    38341 gggactgatg aaggcagttg aaaaatttga ctaccgcaaa ggatttaagt tttcaaccta
    38401 tgctacatgg tggattcgtc aagccatcac tcgagcaatc gctgaccaag cacgtacgat
    38461 ccgtattcca gttcatatgg ttgaaacaat taataaattg atacgtatcc aacgtcaatt
    38521 gctgcaagac cttggaagag aacctacgcc agaagaaatc ggtgcggaaa tggatcttcc
    38581 aacagaaaaa gtccgagaaa tcttgaaaat cgcgcaagaa cctgtttccc ttgaaacgcc
    38641 aatcggtgaa gaagatgact ctcacttagg tgatttcata gaagaccagg aagcaacaag
    38701 tccagcagaa cacgcagctt atgagttact aaaagaacaa ttggaagatg tactagatac
    38761 attgactgat cgggaagaga atgtccttcg cttacgtttt ggtttagatg atggccggac
    38821 gagaacattg gaagaagtag ggaaagtatt tggtgttact cgggaacgta ttcgtcagat
    38881 cgaggctaaa gcattaagaa aattgcgcca tccttctcgt tcaaaacaat taaaagattt
    38941 cttagaataa gcgtggacta ttgaccagaa gagaaatcag taaaactgtt tggtttattt
    39001 taaaagaaaa aaagcttgag actacgcagt ctcaagcttt tttttctata ggaaggaaat
    39061 tcattcatta ttttta
//
LOCUS       DAFBQH010000020        38656 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.020, whole genome shotgun sequence.
ACCESSION   DAFBQH010000020 DAFBQH010000000
VERSION     DAFBQH010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.020"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            154..1860
                     /gene="efbA"
                     /locus_tag="LK694_001071"
     CDS             154..1860
                     /gene="efbA"
                     /locus_tag="LK694_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HBK5051958.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2018..2920)
                     /locus_tag="LK694_001072"
     CDS             complement(2018..2920)
                     /locus_tag="LK694_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK5051959.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2917..3609)
                     /locus_tag="LK694_001073"
     CDS             complement(2917..3609)
                     /locus_tag="LK694_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBK5051960.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3652..4293)
                     /locus_tag="LK694_001074"
     CDS             complement(3652..4293)
                     /locus_tag="LK694_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK5051961.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4331..4645)
                     /locus_tag="LK694_001075"
     CDS             complement(4331..4645)
                     /locus_tag="LK694_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051962.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4633..5985)
                     /locus_tag="LK694_001076"
                     /pseudo
     CDS             complement(4633..5985)
                     /locus_tag="LK694_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5982..6914)
                     /locus_tag="LK694_001077"
     CDS             complement(5982..6914)
                     /locus_tag="LK694_001077"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBK5051963.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6911..7687)
                     /locus_tag="LK694_001078"
     CDS             complement(6911..7687)
                     /locus_tag="LK694_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBK5051964.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7741..10926)
                     /gene="carB"
                     /locus_tag="LK694_001079"
     CDS             complement(7741..10926)
                     /gene="carB"
                     /locus_tag="LK694_001079"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBK5051965.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10931..12007)
                     /gene="carA"
                     /locus_tag="LK694_001080"
     CDS             complement(10931..12007)
                     /gene="carA"
                     /locus_tag="LK694_001080"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBK5051966.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12012..13292)
                     /locus_tag="LK694_001081"
     CDS             complement(12012..13292)
                     /locus_tag="LK694_001081"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBK5051967.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13311..14237)
                     /locus_tag="LK694_001082"
     CDS             complement(13311..14237)
                     /locus_tag="LK694_001082"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBK5051968.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14250..15533)
                     /locus_tag="LK694_001083"
     CDS             complement(14250..15533)
                     /locus_tag="LK694_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBK5051969.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15547..16086)
                     /gene="pyrR"
                     /locus_tag="LK694_001084"
     CDS             complement(15547..16086)
                     /gene="pyrR"
                     /locus_tag="LK694_001084"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBK5051970.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16321..17232)
                     /locus_tag="LK694_001085"
     CDS             complement(16321..17232)
                     /locus_tag="LK694_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBK5051971.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17229..17693)
                     /locus_tag="LK694_001086"
     CDS             complement(17229..17693)
                     /locus_tag="LK694_001086"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBK5051972.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17698..18396)
                     /locus_tag="LK694_001087"
     CDS             complement(17698..18396)
                     /locus_tag="LK694_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK5051973.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18383..18769)
                     /locus_tag="LK694_001088"
     CDS             complement(18383..18769)
                     /locus_tag="LK694_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051974.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18916..20586)
                     /locus_tag="LK694_001089"
     CDS             complement(18916..20586)
                     /locus_tag="LK694_001089"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBK5051975.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20769..21002
                     /locus_tag="LK694_001090"
     CDS             20769..21002
                     /locus_tag="LK694_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBK5051976.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21101..21532)
                     /locus_tag="LK694_001091"
     CDS             complement(21101..21532)
                     /locus_tag="LK694_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBK5051977.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21611..22024
                     /locus_tag="LK694_001092"
     CDS             21611..22024
                     /locus_tag="LK694_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBK5051978.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22062..23210)
                     /locus_tag="LK694_001093"
     CDS             complement(22062..23210)
                     /locus_tag="LK694_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBK5051979.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23332..23928)
                     /locus_tag="LK694_001094"
     CDS             complement(23332..23928)
                     /locus_tag="LK694_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK5051980.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24128..26770)
                     /gene="alaS"
                     /locus_tag="LK694_001095"
     CDS             complement(24128..26770)
                     /gene="alaS"
                     /locus_tag="LK694_001095"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBK5051981.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27158..27589
                     /locus_tag="LK694_001096"
     CDS             27158..27589
                     /locus_tag="LK694_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5051982.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27634..28983)
                     /locus_tag="LK694_001097"
     CDS             complement(27634..28983)
                     /locus_tag="LK694_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBK5051983.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29053..30216)
                     /locus_tag="LK694_001098"
     CDS             complement(29053..30216)
                     /locus_tag="LK694_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBK5051984.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30345..30668
                     /locus_tag="LK694_001099"
     CDS             30345..30668
                     /locus_tag="LK694_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5051985.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30851..32200)
                     /locus_tag="LK694_001100"
     CDS             complement(30851..32200)
                     /locus_tag="LK694_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5051986.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32269..33222)
                     /locus_tag="LK694_001101"
     CDS             complement(32269..33222)
                     /locus_tag="LK694_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBK5051987.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33324..34514)
                     /locus_tag="LK694_001102"
     CDS             complement(33324..34514)
                     /locus_tag="LK694_001102"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBK5051988.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(35159..35842)
                     /locus_tag="LK694_001103"
     CDS             complement(35159..35842)
                     /locus_tag="LK694_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK5051989.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            complement(35923..37419)
                     /locus_tag="LK694_001104"
     CDS             complement(35923..37419)
                     /locus_tag="LK694_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBK5051990.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            complement(37465..38631)
                     /locus_tag="LK694_001105"
     CDS             complement(37465..38631)
                     /locus_tag="LK694_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBK5051991.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
BASE COUNT        10950 a         8432 c         6761 g        12513 t
ORIGIN      
        1 ctaaaaattt ggatttcaac gatagaaaca ctgtttttta tgtaattagt ccaagatttt
       61 tctagacttt tgctttacta ttcggccaaa ttggtttttc ttcttttttt cgttataatg
      121 aatatactta tgaaaagaaa tgaggaatga accatgtcct ttgatggtgt atttacccac
      181 gcgatggtca acgaattgcg tgaaacgtta ctatctggcc ggatctcgaa aatccatcag
      241 ccttatgaaa atgaaatcgt gctggtgatt cgttcacgcg gcaaaaacca tcgattatta
      301 ttatctgcgc acccaagtta tgctcgtgta cagattaccc aaatcgatta tcagaatcct
      361 gataatccgc ctaacttcgt gatgatgctt cgaaaatatt tggatggcgc tattttagaa
      421 gacatcgaac aaatcgaaaa tgatcgagtg atccatttcc actttgctaa acgaaacgag
      481 ttgggtgatc tacaaaatat catattgatc gttgaattaa tgggacgtca tagtacgatc
      541 gtcttagtta atcgagaaac tggaaaaata ctggatgcga tcaaacacat tggaagctct
      601 caaaacactt atcgttcttt gcttcctggt gtagaatatg ttgctccgcc aaaacaagag
      661 gtcttgaacc catttagctc cgagaaagaa aaaatctttc agcggctttc ccaaactgaa
      721 ctagatccaa aagcaatcca acgtcagttt cagggaatcg gtttcgatat cgctcaagag
      781 ctgaccaaaa gattactgga acgtcctaat gaaaagatgg ttgtatggga cgaatttttc
      841 tcagccatca gccatcagcc tattcctact ttttacgaaa cggaaaataa agattttttc
      901 acaccgattg cttatcaggt attgtccgaa caggcatctg ctgtaacagc ttatccaaca
      961 ctcagtcaat tattagatag ctattatcac gaaaaagcag agaaagatcg ggcaaaacaa
     1021 caaggcggag aactgattcg aaaaatcgaa aatgaactaa aacgaaataa aaataaattg
     1081 aaaaaacgtg aacaaacact caaagaatcc gaaaacgctg agaattaccg tagaaatggc
     1141 gaactactta ctacttttct gacacaagta ccacgcggcg caaaagaagt tgttttacct
     1201 aattattatg aagaagatcg gccaatcaaa atagcactag atccggcatt gacaccaaat
     1261 caaaatgcac agaaatattt ccatcgatac caaaagctga agaacgctgt taagctgatt
     1321 ggcgaacaaa tccaagaagc caaagatgaa atccaatatc ttgaatctgt cttatctcaa
     1381 ctggaaattg ccggcccaat ggatatcgaa gcaattaaag aagaattgac ttcagaagga
     1441 tacctgaaaa agaagaactt aaaaaaacaa aaacgtaaga aaccatctca gcctgatcaa
     1501 tacttttctt ctgacggaac gctcattctt gttggtaaaa acaatcttca aaatgaccag
     1561 ctgacaatga agacagctaa aaaaaccgac tattggcttc atgcaaaaaa tatacccggt
     1621 tcgcacgtca tcattaaaag tgacaagccc tcagatgaaa caattacaga agctgccgag
     1681 ttagctgcct atttttcaaa ataccgatac tctgcccaag tgccggttga tcttgtacag
     1741 gtaaagcata tccgaaaacc aaatggcgcc aagcctggtt atgttattta tgaaaaccaa
     1801 aagacgatta tcgtgacacc agaagaagaa aaaattacag ctatgaaaca aaacaggtaa
     1861 tcaatgagtg tttctagaat catatttgcg tactttccca caacagcaat tagttataaa
     1921 ttgtttgcta caaaaagagg agttgtgaca aaattctgtc acaacccctc ttttttgatc
     1981 aacggtgttc aataaacagc gttctcatct gcttttctta gctctgctgg tccagtaacc
     2041 gagtaagctc tttgataaat ctttgagcga tttcactgag cggacgtttc gccgaataaa
     2101 tgatataaaa ttcatttagt tcgccttctg ccaaagggat taaccgcaag tgctcgcgaa
     2161 tccctccaat cacaatacca gatcctgaag cgtaagcatc agtttcaaga acgatattca
     2221 tcaaagtacc tcgatcactc gtataaatca ctgtttcttg atcagggatc tcgataaggt
     2281 cttcataaaa atacgggaaa ttatttccat cctgtgtaaa acgaacttga ggataagata
     2341 acaattcttc tttggtgatc tctgcgtgtt cagctaatgg atgtccttgc cttaaaaaaa
     2401 tatgcgtttt gaattttcca atgctgcggc cacttagctc tccttgttgt aaatatcttc
     2461 tgataccagc ttgattatgc tcattcatat aaataatgcc gatctcactg tgctgctctt
     2521 tgacttcttc gatcactctc gctgttgtcg tctccatgat ccgaaattgt ttgatctcgt
     2581 ctttgaattc ctcgagtagc tgtgcggtca aaggacctaa gaaatcataa tgctgggcag
     2641 cgatcgaaaa attgtctctc ttttcactcg ttagatagtg agtttctaat gtatcaagct
     2701 ggaccaatat tttctcggca cgatttaaga agtcttgccc ttcttctgtt aaatatgccc
     2761 ctcgattcgt tcgagtaaac agcgtgatcc ctaactcggt ttctaattct ttgataccag
     2821 ctgaaagact tggctgggag acaaacattt tctttgccgc ttctctgaaa ctaccattgt
     2881 ttgctacagc gaccacgtat ttcatctgct gaatattcat tgctcaaact cctcgaaata
     2941 ataaatttct cttccattca agggctggat tggtcggttg ttcggtcgag catactgacc
     3001 atcgaagggg agaagaaact cttggttcag ttttcctaca tgatccatta caaaccattg
     3061 gattggcccg ctggtaggcg gagttgtcaa tgagccaata taatggaaat ggcttttttg
     3121 ctccggaaag aacacatccg gatttaccca atgctcatgt ttttctaagt tccaactttc
     3181 cccattttct tgattataca gccaaccttc ctgtaccatt agctggtaca tgacacccac
     3241 aactagattc tcttttttgc tgttcatatg aacaaaatga aattcaatat cttcctgttt
     3301 tccgttgatg atatgttcag aaggcaagtg gaaatgaata tctgtcaaat aatagcgttc
     3361 tttcttgaac tctacataac tgagttggtc aaaaggtact aaatgaatcg tgtttttgaa
     3421 ttccttgtca gtaaaccgtt gcttttgata atgaaaggaa agcgtgtctc cttctatttt
     3481 ggtttcttcc cccttggtca aatgaatagg cgactgcaat ggaaattccg cacctctcgc
     3541 aaaccaatca cataaactcg cccaattttc cggacctttg tctccagaat agccccattc
     3601 gacatccata ttctttttca actatacttc cccctgcatc tcatatcatt tttatttttc
     3661 ttctttcttt acaatcgttg agacggtttc taaaaacgcc tcttttccat aaaattcctg
     3721 gttcaatagt tccggaaata tctctttaaa gaaatactga acagaaggcc gatgttcaaa
     3781 gcttttaatc gtagtcactg cctcaatgaa catctcctta taaacttttt ccgggttatt
     3841 tttgatgatt tcatcaaaag attcataaag aaggtcaacc ttatctgaaa tggctaatag
     3901 ttctccttct acggtttcat cttttccttc gaacaaccga cggcgataaa cttcttggaa
     3961 ctcagctgga atctcttggt tgatgaattc gtccgtcatc ttttcttcaa ctgtttgcag
     4021 catccctctt aactctttgc ttgcgtattt caccggcgtt ttgatatcac caataaaacg
     4081 ctctgtgtag tcatgattca atgatttttc atagagcagt ttccaatcga tcggttgtcc
     4141 attcgcttct tcaatgtctc ctagaacttg cgcaatagat gctacacgat acgaatgtgc
     4201 tgcaactgta tgctctgtat atttaaaaaa tccaggtgcc cttgtgatcg tctctaaatt
     4261 gttcaatcct aaaataaatt cattcaaccc cataaaagtt acctcccttt gagatttctt
     4321 tttccgaata tcatatcatt aaataactga tattcgcaac atttccgctc tttaccattt
     4381 ttgaaacagc tcccttacca aaaaatagag aaaatctata tataatagaa aaaagagaag
     4441 agattattct cttctttctg ctaaccagtc ttagaaaaat gaattttaaa aaaatcaaaa
     4501 actgatcacg atagaagttt cttttcatac ctgatgaaaa gatgcttctc gcaatcagtt
     4561 tttggatttt tgctattcca attgatgatt ctggagacac aaatcggatt tctctatctt
     4621 catcaagcgc gcttaaatct gccaattttc aggatctttt ttccattctt ttagcagagt
     4681 taattcttgc tcatcgatat aacgatcttc taaagctgct tcaatcaata cagaataatt
     4741 ggtcaacgtc ataagaggga tttcggcttt ttcgaaattt gcttttcctt ttggcaactc
     4801 atacgtaaag atagccgcaa caccaaggat atccgcacct tctctttttg ctgcttcagc
     4861 tgcttccaag acacttccgc ctgtagaaat caaatcctcg atcacaacca tcttctgacc
     4921 ttcaaatata cgtccttcga tttggttacc tttcccatga tctttcgcct tacttcgaat
     4981 atagaccatt ggcaaatcca gtatttccgc tacccatgcg gcatgaggga ttcctgctgt
     5041 tgcagttcca gcaatcactt gtacttcagg gtaagcttct ttgattttgc ttgctaatcc
     5101 ttttgcgatc tcttttcgga cttttggata gctcatcgtg attcggttat cacagtaaat
     5161 tgggcttttg attccacttg cccatgtaaa aggattactt ggatttaaaa atactgcttc
     5221 aatttctaat aagtcttttg cgatactttg ttcaacattc attttaggtt agctccattc
     5281 tgtttgaatc gtttgatatg ctttataagg attagttgac tgagtgattg gtcgacctat
     5341 aacgataaaa gttgagccga tcttttttgc ctgagaaggt gtgactactc ttgtctgatc
     5401 tcctgcgact gttccttctg gtcttatacc cggagttaag caaacaaatt cgtctcctgc
     5461 catttctttg atcgcttcta cttcccaagc agaagaaacc acaccatcta atcctgcttt
     5521 tttggcacag ctagcataat ggatcacact tttttctaaa gaaacttcga ttagttgatc
     5581 ggcatgcatt tcttcttcac ttgtagaagt aagctgagtg atagctagaa gtagcggacg
     5641 ttttccacct tctggagtcc cctcttctaa tcctcgcatt gccgcctcca tcatacgaat
     5701 accgccagcg gcatgaacac aggtgagatc aacaccaagc tttgccaatc cccgcattgc
     5761 tttttcgacc gtattcggaa tatcgtgaag tttcagatct aaaaatacat tatgaccagc
     5821 ctcttttaga taacgtacga tttcaggtcc ttcttggtag aacaattcca ttccgatttt
     5881 cacaaacaat ttttcttctt tcgggaaaaa tcgaagaaag tcttctacct cttgcctagt
     5941 tgaaaaatct aatgcaataa ttggtcgttg gtctagttga ttcatcgttc acgttcctct
     6001 ctcacttctt tgattaatct ttccagcgat tcgatgccta gttcgctcat tcgcacaggg
     6061 agctctttaa ttagtttagg acagatatac ggatcggtga aattcgcggt ccctatagct
     6121 acagcacttg ctccagccat aaacatctca agaacatcat ccactgtctg cacgccgccc
     6181 atcccaatga ttggaaggct ggatatactt gctacttggt gaatcaaacg aatagcgaca
     6241 ggcttgattg ctggtccgga gagtccacct gtttgattgg ctaaaatcgg ttttcttgtc
     6301 ttaaggtcaa tccgcatacc aagaagagta ttgatcatcg taaacccatc tgcccctcca
     6361 gcttcgattg cctgagcgat tggaacgatg tctgttacat ttggggacaa tttgacatag
     6421 acaggaacag aagaaacttt ctttactgct tgtgttaatt gaaaagcaac atcagaatct
     6481 gttccaaaag caattccgcc atgtttgaca tttgggcaag aaatgttcag ctcgattgct
     6541 ttcacatttg gtgcttcgcc aattttgcta caaacttcta catagtcttc ttcacaagca
     6601 ccagcaacat ttgcgatgat gggtaactca ttgtactcct ctaattctgg aagaagttta
     6661 tgcataacaa cgtctagacc aggattttgc aaaccaattg cattcaacat gccagatggt
     6721 gtttccgcca ctcttggcgt gggattgcca aagcgggcat ttggagtcgt tgccttgacc
     6781 atgatcgatc caagctgatt caaatcatag tattttccgt attctttgcc aaaaccgaaa
     6841 cagccgcttg ccggcatgat aggatttttt aattctaggc caggaagttt gaccgctaaa
     6901 ggattctcat tcatataatt acctctccta ttttaaaaat cggtccttcg tcacaaactt
     6961 tcacactttt tgttcctgtt tcatcttccg gcgtatgaca aacgcaagca tagcaagccc
     7021 ctatcccaca tgccatgcgg gattctaatg ataactggac attttgatgc gaagaaaaca
     7081 gctgttccac tgtcttcaat aacccgtttg aaccacatgc aaaaacagct gtaggttccg
     7141 tcttcgttcc taacaataaa ttaccgacat ttccgtgcat accgtatgtt ccatcgtcag
     7201 tagcgattct tgtttctcct agacgattaa attctttttc ataataaacg acttcatgtg
     7261 tggcaaaacc taaaaaatgc gttggcttaa ttcctttttc gactaactgt tttgataact
     7321 catatagagg agggacaccg attcctccac cgattataaa tacttcgtct cctgtatgaa
     7381 gattggacaa atcaaaacca ttgcctaaag gacccatgac atctaaatgg tctccggcag
     7441 aaagagtaga gaagactcta gtcccctcac cttccacccg ataaatgata cggcaagtat
     7501 ttgtttcgtg gtcgatatga ttcaagctaa tcggtcggcg taatagcaag tctgcacgcg
     7561 gcacgagaat atgcaagaac tgtcctggtt gctgcatttc ttttactaga tcacctttca
     7621 atgtcatttc aaaaatcttt ggtgccagct gtttttgctg aacgatttcc atcatttctt
     7681 gtttcatctg tcccctcctg caacggtttt tcgtcttttt gtcttaacat gtgctcgccg
     7741 ttaaataggg ttagttgaaa aagcacggga ttccatcact cgaacgatcg catctgctgt
     7801 gtctaaagaa gtaaacaatg gaataccatg ttcaactgct tctcttcgga tgatgaaacc
     7861 gtctttagaa gcatttcccc gatctttatc aatcgtattg acaaccactt gtgttttacc
     7921 atctcttatt aagtcgacta catttttttc tgctgcttcc tcagaaattt tcgctactgt
     7981 ttcaacgacc agaccatttt tttcgaaata agcagctgta tgtttcgttc ctatcagact
     8041 atagccaatt tctgtaaaac gtttagctaa ttctagtgct tcttcttttg tttcgtccgc
     8101 aatcgtaaat aatacggctc catattcagg caagtgtaat ccagaagctt cgaatgcctt
     8161 gtacaaagct ttgtctaatt gtctgtcaga tcccattact tctccagtcg atttcatttc
     8221 aggccctaag taagtatcaa ctttctgcaa tttcgtaaaa gaaaagacag gtgctttaac
     8281 atgaacttct tttgtttctg gataaagtcc gttttcataa ccaagtgtct ttagtttctc
     8341 tcccaaaata gctttcgtcg caatttgtgc cattggaatt cctgtcacct tacttaagaa
     8401 tggaacagtc cgacttgctc gaggattgac ttcgatcaca taaacctcat tttcatggat
     8461 gacaaactgg atattcatca tcccgacaca attcaaacct aatgctaagc ggatcgtgta
     8521 atcttcgatc gttttcttga tcgattcaga taatgtttga ggtggataca cagccattga
     8581 atcccctgaa tggacaccag cacgctcaat atgctccatg atccctggga tgagtactgt
     8641 ttcaccgtca caaatcgcat ccacttcgca ttcaccacca agcaagtaac ggtccactag
     8701 tactggatgt tctggagatg ctttcacagc atgttccatg taatcttcta aatctcgctg
     8761 gttttcaacg atttccatcg cacgtccgcc taatacatag cttggacgaa caagaactgg
     8821 ataaccaatt ctcgaagcga taaccacggc ttcttcttta cttgtagcag tatcccctaa
     8881 aggttgagga atatccaact ctttcagcgc ttgctcgaat aaatcacgat tttccgcacg
     8941 atccaagtct tcgatcgttg tccctaagat ttttacacca gcagcagtca aaggttcggc
     9001 taagttgatt gctgtctgac caccaaattg aacgattact cctatcggct gttctagctc
     9061 gatcacattc atcacatctt cgaaggtcaa tggctcaaaa tataatttat ccgaaataga
     9121 aaagtcagtc gaaactgttt caggattgct gttcatgatg attgcttcgt atcctgcctg
     9181 ctggattgct ttgacagaat gcacagtcgc atagtcaaat tcaacccctt gtccgatacg
     9241 gataggacca gatcccagta ctagaattga gggcttttct gatcgtgaac tttcattttc
     9301 atattcatag gaactataaa aataaggtgt ttttgattcg aattccgctg cacaagtgtc
     9361 aaccatctta tatacaggaa tcaaatcatt ttcttttctc atctggcgaa cggcttcttc
     9421 gtttatttcc cacagctccg ccactttttt gtctgtaaaa ccgttttgtt tcgctgtttt
     9481 caatatcttg tcatccaacg gctgctgttt caactgctgt tcgatttcta cgatatgcaa
     9541 cagtttatct aaaaagaata aatcgatttt tgtcaaatcc gctaattctt ctatggtgta
     9601 ccctcttcta atagcttcag ctacataaaa caggcgatca tcttgtgctc gaaccacttt
     9661 ttggattagg tcatcgtccg tgatgtctgc caactcagga agttcgatat ggtgtgtacc
     9721 gatttctaaa gaacgcacag ctttcaataa agactcttcg atattccgac cgatagccat
     9781 cacttcacca gttgctttca tctgcgtgcc taaaagccgt tctccgtttt caaacttatc
     9841 aaacggccag cgaggaattt tagctacaac ataatctaat gcaggttcaa attctgcaaa
     9901 agtcgttcct gtaacaggat ttttcatttc gtctaatgtt aatccaagcg ctattttagc
     9961 agctagctta gcgatcggat agccagtcgc cttacttgct aacgcagatg aacgagaaac
    10021 acgcggattg acttcaatca cataataatt aaaactatgt ggatcaagag cgagttggac
    10081 attacagcct ccctcgatct taagtgctcg aataatcgaa agagaagcat cacgaagcat
    10141 ttgatattca tggtcagaca atgtttgact aggggcaaat acgatagaat cacctgtatg
    10201 gatacccacc gggtcaaaat tttccatatt gcagacaacg attgcattat cagccgaatc
    10261 tctcatcact tcatattcaa tttctttaaa acctgcaata cttcgttcaa tcaaacattg
    10321 ggtagcgggg gaaagactta atccattttc tgatacgata cgtaattctt cttcattttc
    10381 acacatacct ccaccagttc cgcctaaagt aaaggctgga cgaacgatga caggatagcc
    10441 gattttattt gcaaaatcta cagcttcttc tactgtattg acgatttcag actcggggat
    10501 tggctgattc aattctttca tcaactgttt gaatagatca cgatcttctg cttgatcgat
    10561 cgcagaaagc tttgttccta gaagttctat gttcaattct tctaaaatac ctgatgctgc
    10621 cagctccatt gccatattga ggcctgtctg tccgcctaaa gttggaagga gagcatctgg
    10681 acgctctttt cgaagaatcc ttgatacaaa ttcaagtgtg atcggttcga tatatacttg
    10741 atctgccact tctttatccg tcatgatcgt cgcaggatta gaatttacta aaaccacttc
    10801 atatccttct tcttttaagg caaggcaggc ttgtgttcct gcgtagtcaa attctgcggc
    10861 ttgaccaatg atgatcggac cagatccaat caccataatt ttcttgatat ctgtacgttt
    10921 cggcatctgt ttactgctcc tttcctgcgt ccatcatttc cataaattca tcaaataaat
    10981 gtagtgcatc atgcggacca ggggcagcat ctggatgaaa ctgcacactg aaagcgggat
    11041 aatctctgtg tttgactcct tctactgttc cgtcatttac ttctacatga gttacgatga
    11101 gtttttctgg atcaatcgtg gcttcatcga ctgcatagcc atgattttga gaagtaaaat
    11161 caattcttcc ggtcgctact tctcgtaccg ggtgatttaa tcctcgatgt ccgaatttca
    11221 ttttataggt atctgcaccg ttggctaaag caaataactg gtgaccgaga caaataccaa
    11281 aaatcggtac ttttccttgg atttttttga tcatttcgat tgcttctggc acatcttttg
    11341 gatcaccagg tccattcgtc agcatgactc cgtctggtga tagcgctaaa atcgtctcgg
    11401 catctgtgtt atagggaagt actgtgaggt tacagtttct tttactcaat tctcgtaaaa
    11461 tactatgttt caagccgaag tcaatgacaa tgactttccg acctgttcct ggacttggat
    11521 aaggtttgtt agttgagact tgctgcactt gattggttgc tagaacggtt gctttcagct
    11581 gatcaaatgc atgttcgaag gaatctccag catcaactat actagctttc atcgttccga
    11641 cttcacggat tttgcgagta agggcgcgcg tatcgatacc tgatattcca ggaattcctt
    11701 tttgtttcaa aaatgaatct agcgtcattt ggtttcgcca gtttgaagct acacgtgcat
    11761 gttcttttac tactactcct ttgcatgttg gagaaatcga ttcataatca tctctattta
    11821 ccccataatt acccaccata ggataggtaa atgtaatgat ttgaccgtta aaactttgat
    11881 cagtgatagt ttcttgataa ccagtcatac ttgttgtaaa aacgatttct ccgaagacac
    11941 tagcatctgc accaaaagcc tttccttcga aaactgttcc atcttctaat ataagcaaac
    12001 gttccatact tttattctcc cttctgccac acaagttttc cgttgacaaa agtcatttgt
    12061 gtttcgcctt ttactttcca gccgataaaa ggtgtattct ccccttttga taaaaagtct
    12121 tctttatcga tcgtgcaagt ttgtgtaata tcaaaaactg ctacatcagc gggggcacca
    12181 accgttagcg ttcctgcatt caaaccaaaa atctcagccg gtttaactgc caaccaattg
    12241 atcacttgtt ccaacgtgaa tcgaccagtt tctacaaaat gagtatagat cagttgaaac
    12301 gctgtttcgc ttcctacgat accaaatggt gatttcatga aactttggct tttttcttct
    12361 aatccatggg gggcatggtc tgtagcgatg caatcgatcg taccgtcaag caagccttcg
    12421 atcaaagctt ctctgtcctc ccgtcctctt agcggcgggt tcattttcca aaagccgaaa
    12481 tcttcgggaa tatcttcgtc gatcaaaata agatgatggg gtgaaacttc tgctgttacg
    12541 tggattcctg ctttttttgc gtctctgatt acacggacac tttctttagt agaaacatgg
    12601 caaacatggt aatgaacacc agttgcttca gctagcaaca gatctctagc aatttgagaa
    12661 gactcggtta cgcttaagat cccgggaaga ccaagttctt ctgccttctt gccagcgtgc
    12721 ataacgccgc caaataataa agattcatct tctgtatggg ctactagggc tttattgttt
    12781 tttgcagctt ctttcatagc cagatacatc gtcccagctg tttggacacc tacaccgtca
    12841 tttgtgaaag caaaagcacc ttcttcaatc agtgcttctt gatcaactaa tttctcactt
    12901 cgcagatttt ctgtgattgg cgcatactgc aataccttca caactgcatc ttttctaatg
    12961 atgtcatata cttgtctcag cttttctgcc gtatcaggta caggatttaa attcggcatc
    13021 gcgcatacag tagtgaaacc gccacgagca gctgagcgag tcccagcttc gatcgtctcc
    13081 ttgtatgtaa atcctggctc tctcaaatga acatggacgt ccactaaacc aggtgtgatc
    13141 aattgtccct ttgcatcaaa cacttcatca aattcggctt ctgagaatcc tgtaccgatt
    13201 gctttgattc gcccatcttc tatccaaatt tctgctggtg tcgtcatatt ttttcgtgta
    13261 ttgatctgac cgtttttaat gagtgttttc attttttcat cctcctataa ttacgctttt
    13321 ccatttagca ctgcttctaa gattgccatt ctgacgaata ctccattgct catttgctga
    13381 acgatccgcg actgtaatcc ttccactaat gaatcagcta attctacatc acggttaact
    13441 ggtgctgggt gcataatgat tgcatgtttt tgcaacttct tcgcacgctc aactgttaac
    13501 ccatgttcct gatggtagcc ttcttttgag aaactttctt ttccatcatg gcgttcgtgt
    13561 tgcacccgaa gcatcatcat gacatcaact tgatctagta gttcatccaa tggcatataa
    13621 tgtccgtaca cttcgaattc atcgtcgtac cattctctag gacctgagaa gaatacttgt
    13681 gcgcctagac gttttaacat ctgcatattg gatttagcta cgcgagagtg agtgatatca
    13741 cctacgatgg caactttcag accttcaaaa tgaccaaatt cttcataaat ggtcatcaaa
    13801 tcaagcaagc attgggttgg atgctgtccg cttccgtctc ctccattgat aatcgaacac
    13861 tggattgttt tactttgtat cagttcgtca taatagttct catcaccatg acggataacc
    13921 gctacgtcta cgcctaatgc tgacatcgtc agtactgtat cgtatagtgt ttcccctttt
    13981 tggacagagc ttgtgcttgc ttcaaattca atcacttcta gccctagttt tttctccgct
    14041 acatcgaagc ttttatgtgt gcgagtacta ttttcgaaaa agaggtttgt agcaaagtac
    14101 tggcgctttt gcggagtcca attagcacct tgcttgaatt cctgccctct gcggatcaag
    14161 cccataactt cttgatcagt aagtgcctca acggtcaata aatgctttaa gctgatacgt
    14221 tctgatttga tgatcataat gtttcctcct tagttttctt cgcttcgtga agtttctggt
    14281 aagataagat ttaaaatgat gccaagtaca gttgctaatg ccattgccga caatgtaaat
    14341 gtgcctactt caaagacgag tccgccaatt cctactacta agataactga agcaatcaac
    14401 agatttttct ttttatcaaa atcgattttg ttctcgatta aaatcttcat tccacttgcg
    14461 gcaattactc caaataagat aaaactgatt cctgagatga ctggtccagg gatacttaaa
    14521 atcaaagcgc ttaattttcc tacgaatcct aaaatgactg caaatactgc agctccaccg
    14581 atcacaaaca cgctatggac acgtgtgata gccagaacac caatgttttc accgtaactt
    14641 gttacaggag gacctccgac aaaggctgcc acgatttgtg ctaatccatc acctgtcaac
    14701 gtcttatgaa gacccggttc ttcaaagaaa ttacgtttag ttagtttatt caataccata
    14761 agatggccga tatgctctgt catcgtcaca aaagcgatgg gtgccattgt tgtgattgct
    14821 ccaagatata gctttggttc atattgtaca aagggaatct caaagttagg aacagcaaac
    14881 caggcagcgt ctacgattgg cttagtatcc acgattccaa acagcaaggc tacgatgtat
    14941 ccgctaataa tacctagtaa aatcggaatc aatccgagaa aacctcttag ccacatgttg
    15001 aagaaaattg ttaatcctaa tgtgatcagc gctacagcaa tatacttgaa atcatacgtg
    15061 ccctgattat acatagcatt tgttgctgca tttgccgcaa gtcccaaccc gataaccatg
    15121 atgactggac cgacgacgat tggaggcaag attttatcca gccatgctga accgatcttt
    15181 ttgatgatca gcgcaacaat caaatagact aatccagttg tgactgctcc ttgtgcaatc
    15241 gctggataac catctgtttt catcaaaagc tgcatcgctg caatgaaagc gaagctgctt
    15301 cctaagtacg caggtatctt tcctttagta gtaaataagt acaccaatgt acctagtcct
    15361 gaactgacta gcgcaatacc tggatcgata cctactaaga tggggactaa cacggttgct
    15421 ccgaacatcg tgaacaagtg ttgtaaactt aatcctaccc aatgtcctgg ggttgggcga
    15481 tcgtggatat ctaacaccac gtcatcatta tgaaatcctt ttttctccga catgtttctc
    15541 tctcctctaa tcttcctctt tcaaaatcat gatgcgatct tgtccatcaa gctcttgcat
    15601 ctctactaaa atctcttccg cccgagatgt tggaatgttc ttcccaacat aatctgcacg
    15661 gattggcaat tctcgatgcc cacgatcgac caggactgct aatgaaattt ttcttggtct
    15721 gccaaaatcc attactgcgt ccattgctgc tcgaatcgtt cgtcctgtat acaaaacatc
    15781 atcaatcaaa ataacttctt ttccttctaa ggaaactggg atgtcagaag aatgaagttc
    15841 cggttcttct ggattttctt ttttgtcatc tcggtataaa gtgatatcca gttctcctac
    15901 aggaatatcg atatcttcta gctgcttcag gcgttcggca attcttgatg caatgtagat
    15961 tcctcgtgtt ttgataccta ccaatacgat atcttggata ctatggtttc tctcgataat
    16021 ctcataagta attcgagtca atgctcgctt catggttacc tgatctacta cttcttttgc
    16081 ttgcattttc attcccccta cttttttgca taaaaaaact cctgccctaa gaggacagga
    16141 ggaatgttta gactgagcgt atactgatac actgatagct atactatgtc aatgagcaat
    16201 ctacttatgg cgcaccatac gtatccttgc tcccaccctt cttagcctca cgggactaaa
    16261 cttaaaggaa ttctattaag ttttatgctt tcgttgttaa ttactatacc gttatctaaa
    16321 ttaaaatgca atgttttttc ttaatttttc taaagttttt tcaaaaactt ctggaagagg
    16381 tgcttcaaag atcatctttt cgtttgtagt cgggtgcgta aaacctaaca tttgcgcatg
    16441 gagaaactga ccattgcctt tcaaagtttt cttcggtccg tatactggat ctccagctaa
    16501 cggataaccg atatacttca tatgcacacg gatctggtgt gttcttccgg tttctagttt
    16561 taattcaacc agagtaaatc cttcgaaacg ttccaataca gtaaaatgtg tgacagccga
    16621 cttcccatct tctcttatcg cctgcatttt ccggttttcc ttcgaacggc cgattggcgc
    16681 attgatggtt cctttttcgt gaggaatctc tccatggact aaagcaacat atttgcgcaa
    16741 agaggtctta tcttttaatt gctgagctaa tgattcgtgt gcctggtcat ttttggcaac
    16801 cattagcagt ccggaagtat ctttgtcgat tcggtgaaca atacctggac gaataacgcc
    16861 gttgatcgta gaaagatcct tgatatggta catcagcgca ttgaccattg tgccatgcgt
    16921 atgacctgct gaaggatgaa cgaccattcc ttgaggtttg tttatcaccg ctacatcttc
    16981 atcttcgtaa acgatcgtca acgggatatt ttctgggacc acatctaatg tttctggttc
    17041 ggggatcaag acagtaatct cgtctccttg ttttactttg taatttgctt tgactggctg
    17101 tccattgaca gaaaccgctc catttttcag ccacagctga atttgcgaac gactgtaatc
    17161 ggtgaataaa ttagtcaata ctttatcgat cctaccagat tcatggtgaa tcgtcgtttt
    17221 aagttctgtc atttttttct ttgatccttt tcatctaaaa taatataaat taacatacaa
    17281 atcactccac atacaagtgt cacatctgct acgttaaaaa tcggaaagct cataaaatct
    17341 gtctggaaca tatctactac atagccaaga cgaatacggt cgataaaatt gccaatcgca
    17401 ccagctagaa tcaaactcaa tccaatcatg aaccagcgat tacctttttc acgattttta
    17461 atcatcaatg tgagaatcac aacgactgca atgagtgtca ctatagtaaa gaaccacatc
    17521 ttcccttcta gcatactcca tgcggctcca gtgtttcgaa tatgattcag tgacaaaata
    17581 cctggtataa gatttttcgt ctctccaagc gctagattat cagtgatcca ccatttgacc
    17641 aactgatcca ctcctactag caaagcgctg aatatccaat aaataattaa caaaaaatca
    17701 atccttttct atttgtttat aaatatcacg aggtcgtacg atcccgagca gattaccatc
    17761 gggattccca tctttagtga taagtacggc ttccaaagtc ccttgctcac gaaacatcgc
    17821 ttcaacctcg taaatatgtg ttttagctgg aacaaaatga taatttgtgt actttccgtt
    17881 ttggatgatc aaatccaacg ctcgtttatt tgttaaatca accggacctt tttggctttc
    17941 ttttgctagc caaaacccga tattacgtaa agtaatcaat gctacaaatt ttcctttatt
    18001 gtataacgga aattgtgaat accttttttc tgctactatc tttaacagtt caagtaacgg
    18061 aagatcttgt tcaaaacctg ttacacgttt tgcaaatcga ggcagcacgg tttcaggctg
    18121 aatcagttct tgttcgatca gctggattcg cttgactgtc caatcatttg gctctgcaat
    18181 gacaaaatcg actgcgatcc gatcatggac gatcgcattc cttaactgag caagctgtaa
    18241 aagatcatct tcgtattttc tgatcattaa ttgttttctt tgagctaggc gtctgacaag
    18301 ttcggtaaat cccatatttc gtgcatttcc catctcgtct ctcatccatt tttcgattcg
    18361 attgaaactg attaaaaaaa gatcagagtt tgccataatt ttcctccttg atcaaatatg
    18421 cccggttttt tatatagaaa aagaacgcaa tcaacacaat aactgcaaaa aaactgaaaa
    18481 ataaattttg taggaaaacg gcccccaacc cagttgctag aaataaaaac ggatagattt
    18541 gaagaatcaa gtaattcatt gtgatcacat aagcaggctt tgcttctttc actgtataaa
    18601 cggggctttt tagccaataa aaggtgacct gcatcgtaaa gggcacttcc agatcaagcg
    18661 ttatcttttg ttcatgactg agaacactgc gtttttcatt gttgattctt agctgaatcg
    18721 agctacccat accgtaaaaa ccggtttttc ttataaatgt cgttttcatc ataccctcct
    18781 attatgcttt agtatagctc aaaatacaat aaagaaaaag aatgatccta gtgtaaacaa
    18841 aattaattcc ctatcgtcta aaaaaagaag ctgtagtcaa aatacagctc cttcgatggt
    18901 agattataat caagcttaaa acaatcccaa cacttctccg tcatttgtca cgtccatatt
    18961 caatgcagca ggttttttag gcaaaccagg catcgtcata acatctccag tcaaggctac
    19021 taaaaatcct gcccctaatt ttgggatgat ttcgcggatc gtgatggtga aatcttccgg
    19081 tgcacctagc agtgaaggat catcagaaaa tgaatactgt gtttttgcca tacaaatcgg
    19141 cagtttgtcc catccattct tcgtaaactc ttctatttgt ttttgcgctt ttttgctgaa
    19201 aacaacacca tttcctccgt aaattttctt gacgattgtt tctgtctttt cttgtatcgt
    19261 ttcttctagg cggtataatg gcttgtaatt agcttctttt ctatcaatca atcgtactac
    19321 agcttttgct aaatcaacgc cgccttctgc tccatctgcc catacactcg ctctcttggc
    19381 aagaatcccc tgattttcac acaagtgctc caacagtgtg agttcattgt ctgtatctgt
    19441 tacgaattca ttgattgcga cgatcacagg tagctgatat tgttccatat tacgtatatg
    19501 gcgctttaaa tttgcaaaac ctactttcaa agcatcaaga ttctcatttt taagttcatc
    19561 ttttttcatt ccgccatgca ttttcaatgc acggacagtg gcgacaatca ccacagcatc
    19621 cggcgctttt ttcaagttag gtactttgat atctaaaaat ttctctccgc caagatctgc
    19681 accaaatcct gcctctgtga caacgtaatc tgctaatctc aatgctgtct tggtagctaa
    19741 aatactattg cagccatgag cgatattggc aaacggtccc ccgtggacaa aagctggtgt
    19801 tccgtaaatt gtttgtacaa gattcggctt gattgcatct ttcagcagta atgccagagc
    19861 gccttctgct ttcaagtctc cagcagtcac aggctgacgg tcaaatgtat agccgacaac
    19921 gatccttgca agacgagcct ttaaatcttc aaggtctgcc gcaagacaca agatagccat
    19981 gatttcgctg gcaactgtga tatcaaaccc atcttctctt ggaacacctt ggattggccc
    20041 acccaagcca acaattactt tacgcagttc acgatcattg agatcgacta cccgtttcca
    20101 aatcactcgg cgggcatcga tgttcaattc atttccttga tgaatatgat tatctaaaag
    20161 agcagataat gcattattag ctgttgtgat agcatgcatg tcaccagtaa aatgcaagtt
    20221 gatatcttcc ataggcaata cctgtgcata tcctccgcct gcagcaccac ctttgattcc
    20281 catcacagga ccaagtgagg gttctcgcaa tgcaataaca gattttttat tgattttatt
    20341 gagcgcgtct cctaacccta cagtgatcgt tgactttcct tcgccagctg gtgttggatt
    20401 gatggaagta actaagatca aatgcccctc agcgttatcc tttgatcgat tgattccaga
    20461 aaaattgatt ttcgccttat atttcccata cagttccaaa tcgtcttcct taagatctaa
    20521 tttactagcc acttcaacaa tcggttctag ctctgtttct tgtgaaattt gcagatcatt
    20581 cttcatttga ttaccccttt tcacgaactt tattcaacaa aatatcttct ttgttaagat
    20641 atgccaatta tacctttttt tattcagaaa aaaaacataa aattacaggt ttttaattta
    20701 tttgacgtac ttataaatac tctttataat gaaggctatg atcagacttt agaaaggtcg
    20761 tgagtgcttt gaccacagga attgtaaaat ggtttgacaa caaaaaagga tacggcttta
    20821 tcagttatga tgacacagaa gaaatttttg tacattttac tgcgattgaa gaagagggat
    20881 tcaaaacttt ggaagaaaat caagtggtcg aatttgagat catcgaaggg aatcgaggaa
    20941 cacaagctgc ccatgtcaag aaaggcaacg ttcagactac cgatactagt atgcatgaat
    21001 agataaacaa atagagtgag acgctaaacg ggatgaaccg tacgtctcac tcttttcttt
    21061 tcctgtaaaa attcgttttt ttaggttctg atttttaaaa ttagcttttt ctttttttcg
    21121 tataaggata aggaattgat tctgatttgg gtccgttttt ttcaaaatga tcaaaaagga
    21181 ctacttgatc tttttttcgc atcatgaaaa cagatggggc tttctcatct tgcaacatca
    21241 tttgcacccc agtattagtt ggcgtcaaag atcgaatctg tccgacagat atttctcggt
    21301 ctttccaaaa agcagcataa cgtataatca cagagtttga ttttaaaata aaagaacgcc
    21361 gcaaacctag aaatagaaaa acaagaaaaa tacctaaaac aaaattgctt ttccaataag
    21421 gacctgtgtt ttctaaagaa agaatcagac taaaaaacag aatgatcaag gtcaatgacc
    21481 aatagatgat cgtatgtgcc agttcaggct gccatcgaat attttttttc attctctaac
    21541 ctctcaccga ttcagtaatg atataatctt accataacat tagtttgaac aaaaaggaga
    21601 atctcttttt atgttaaaag tatacattga tgcctctaca aaaggcaacc ctggtcctag
    21661 tggaggtgga atcttgattg tctatcaagg taaacaagaa cagctatcta tcccgctttc
    21721 tatagggaca aaccatcaag cagagttcga agtcttttta aaaactttag aatttctaaa
    21781 gaaaaacgac ttacagaatg aaacgatttt ctgtttttca gacagcaaaa cactagtttc
    21841 tacggtcgac aaaaacgcaa cgaagaacga gctcttcctt ccctaccttc aaagaatcca
    21901 agaacttcta tccgaatttt ctttattgat cctgcaatgg ttgcccgaaa acaaaaataa
    21961 aggagcagat aatcttgcac gtcaggctct ccataaacaa ttaaaagaaa aaaagactct
    22021 ttaaattaaa aactttaagg agtcttttta tagtttctca tttaatataa aagataattg
    22081 tgatcaaacg taatttccaa ttggtaatct ccgatttttt cttctatttg actacgtaag
    22141 attctttcta gttctgtatc aacaaactgg aatttctcag ggaccactac atcaaattgc
    22201 agcgtttttc cttgatgaat tactctaaaa tcatgaatat taagccctgt acctgtttca
    22261 agaagaattt gctttaactg ccgcaagata ttatagtatt tttcgttatc aacaggcatt
    22321 ggatccaagt gacagacaag ttccacatcc aaagactttt taaagtcttt ttctatcaga
    22381 tcaataatct tatgagccac attcaaattc attgattcat ctacttcgat gtggactgat
    22441 gcaaagcgtt tattgggtcc atagttatgc accaataaat catgatagcc aagtatatca
    22501 tcgcaagcat ttaaatgctc ttccatttct tcaatctctg ctggcgttgg gcgacttcct
    22561 aatagttcgt atacaaaatt tcgaagcatc gtcaatccac tataaaggat atataatgct
    22621 aaagcaaaac ctatatatcc atcaatacgc catccagtta cccattcgat tgctgctgaa
    22681 agcaatacag ccagtgtagt aaagacatca ttataactat ctgttgctgt agctctcaat
    22741 gtttcagaat caatggattg cgcaataacc tgatacattt tgccttgtgc aaatttcaaa
    22801 aagatggaaa tgattaggac aaaaaagacg atgggtgtta aagagacatt ttctggatgg
    22861 atgatttttt cagcagaaga ctctaaaaat tgaaatccta cataagtaat gatgatcgaa
    22921 acaaataacc cactgatata ttcgaagcgt tcgtgtccat aaggatgttc ctgatctgct
    22981 ggctttgcag caattttaaa tccgatcaaa gtcaatatgg atgaagcagt atctgataga
    23041 ctgttcactg catcagccat aatggagaca cttcctgaaa aaagcccgat cattagctta
    23101 gagacaaaca agatcagatt cgtaaccaat cctaagatac ccgctagtac tccttttttt
    23161 gtccgctgtt gcggtatttg tttctttttt tcttcttgtt tcttagccaa gttttttacc
    23221 ccttctagtt tgcttgttgc tcaggtaaaa agatggagag gacgattgtt ttcctctcct
    23281 ctcttccttt ttctatctat cgtgcgtcct attactatta aaagatggtc attattctgg
    23341 tttcaacggt gttgctgcaa ataacgcttt tgctagtaat ggtgttatga ttcccgcacc
    23401 tattgcttct ggtatcccat tgattgctgc aacccctgct aaggttttaa ataaaagagc
    23461 agagtctaca ccataggcat ctgccgttgc acctgtataa aataatccca tgaatcctaa
    23521 cactaagact gtattgacca atgaacccag tacccctgca aaggctgagc tgatcgttat
    23581 tgatttattt ttttgataaa tccagtggaa aaccaagcct gctactaagc caacaagtac
    23641 tctgggaact accgagataa tcggattcgt gaacactaat gtatcaaatg gcgttgttgg
    23701 catggcatag gcacgaacaa ttgtagcgat tccccaaaac aagccaatca ccatgccatc
    23761 ttttgggcct aatactactg cagcaataat tactgtgata tggataattg ttaaactaat
    23821 gaatcctaat ggaataaacc ccagccaagg aaccatcgct tgtacgatga tgattgctaa
    23881 caagatcgca cgtaagacta gtcgaaaggt cttatttcta ctattcattt tttcacccct
    23941 ttttatttat actaatcgta aaaatttctg tcactttcgt caactaaaga ccatccgaat
    24001 ccgaccattt gattagatgc gccatccaat tgaaaaaagt atgagggcaa gacttgtttg
    24061 tcttcgcccc cataatcatg tttcgttcaa tgtttgaact tttgtttttc tgtcaaacgt
    24121 aaacgcccta ttgttgcgca acccattctt tcgcagcttt aagtgcatct ggaataccag
    24181 caggattttt cccgccagct tgtgccatgt ctggacgtcc accaccgcca ccgcctactt
    24241 tcggtgcgat tgctttaatc aagtcgccag cttttagtcc agcttcattt gattctttcg
    24301 tcattgccgc tagcagactg acttttcctt cttgttcgtt tgctaaaaca agaatatctg
    24361 aaatattttt ttgtttccat tggtcagcta attgacgcag ctgattcata tctttcactt
    24421 ttacttgtgc tgcaatgatt gtttttccat tgatcgtttc tggatctttg aatacatcac
    24481 cagcttgttg attcgctaac ttgctagcta attgttcatt ttccttttgt agctctctta
    24541 cttgctgttg caattgttcc gcttttgaaa caacttcttt caattgaggt gatttaacaa
    24601 ttgttgctac ttgttttagt tgtttttctt cttcttcaag tagttcgtac gcttctttac
    24661 ttgttacagc ctcgattcgt cgcacgcctg caccaattcc tgattcagaa acgattttga
    24721 agattccgat gttttctgta tttgctacat gtgtgcctcc gcacagttcg attgaccagc
    24781 cgccaatatt tactactcga acgtcatgtc cgtatttttc accaaacaaa gccattgcac
    24841 ccatgttttt ggctgtatcg atatctgttt caattgtttc aactgggata gcttcccaga
    24901 ttttttcgtt gacgattctt tccatttcag ctaattcatc tgaagtgatt tgaccaaagt
    24961 gggtaaagtc gaatcgtaaa tgtccaggag cgactaaaga accagcttga tttgcatgct
    25021 cgccaagtac ttctttcaat gccttgtgca gtagatgggt tgctgtatgg tttttgatga
    25081 tccgattgcg catcgattga tcgatcaata gatgataggt ttgtccttca ctcattggtg
    25141 cttttacttc tacggtgtgc ataaattgac cattaggcgc tttttgaaca tctgtaacaa
    25201 ctgctacgat ttcgcctact tcattcatga tccatccttg atccgctact tgcccaccca
    25261 tttctgcata gaaaggtgtt tgatcaaaga tcatttgagc ttcaccttct gcgacttcat
    25321 tcaaaagaga ctcaccttta acgatgacca ataattgact atctgattct tgttctgtgt
    25381 atccaatgaa tttactttct actttgatat ctgtcaataa agcagattgt acccccattg
    25441 atttttcagt actgcgggca gagcgagcac gttcgcgttg tgcatccatt tctttttcaa
    25501 aaccagcatg atcaactttc agtccttcat cttctgccac ctcttccgtt aattctaccg
    25561 ggaagccata tgtgtcgtat agtttgaaga tgtcttttcc tgctagagtc gtcccgtttt
    25621 cgttttttac ttcttggatc acttgattta agatttccaa accttcattg attgtttcat
    25681 tgaaacgttc ttcttctgta cggataactt tttcaataaa ttctttttgt tgaagtactt
    25741 caggataata acttaccatg atttccccaa caacagggac taaacgatat aagaatgctt
    25801 gttcaatacc taattttttc ccatgcatga cagcacgacg aagcaaacgg cgtaagacat
    25861 accctctccc ttcattcgat ggcaatgcac cgtcgccaat agcaaatgaa agggcgcgaa
    25921 tatgatctgc gattactttg taagaaatat ctgttactgg gttttcacca tattttactt
    25981 gtttgcttag cttttctacg gcgtggataa taggcataaa caagtctgtc tcaaagttcg
    26041 ttgacgcatc ttgaacgatt gagaccatgc gttccaatcc catccccgta tcaatgtttt
    26101 tatgaggaag tggttcaaat gtgttatcag gcttatggtt gaattctgaa aatactaagt
    26161 tccagatttc taaataacgt tcattttctc ctcctggata gttttcagga tcgtcttctg
    26221 ctaaatcgtt ataagcttct ccacggtcat agaaaatttc cgtatccgga ccacatgggc
    26281 ctgcaccgat atcccagaag ttgtcttcta catcaataat gtgatctagt ggcaggccaa
    26341 cttcttcatg ccagatgcgt tttgcttctg tatcttttgg ataaactgtt acataaagtt
    26401 tttctggatc gaatgccatc cattttggat cagtcaaaaa ttcccatgcc cagtggatgg
    26461 cttcattttt gaaatagtca ccaatcgaaa aattacccag catctcaaac atcgtatggt
    26521 gccgtgctgt tttaccgaca ttttcgatat catttgttcg aatagatttt tgggcatttg
    26581 tgattcttgg attttctggc acaactgaac catcaaaata ttttttcaat gttgcaacac
    26641 cagagttgat ccacaataag gttggatcgt tcacggggat caaagacgca ctaggttcaa
    26701 tcgtatgtcc tttagattgg aagaaatcaa gaaacatttg tcgaacttgt gcactactta
    26761 attgtttcat gtcattacct gctttctata aattaaatac aaaaaaacac catcacagcc
    26821 aaggacgaaa actcgcggta ccaccttgat tgcaatgatg tgtaatcatt acctcttaaa
    26881 ctcgataacg ctgagctgcg tcggtatttc taccaaaata gaaaaaggga gcaatttgat
    26941 tagtgactat tcttttttca gccaagaaag aattttctgt gagtcttaaa aaacaaatca
    27001 tatccttttc gtacttattt agtatacgca ttctattgat cactgtcaat atctcaattt
    27061 ttattttcat ttccttttgg tcatactcca cgatttttgg tacgataaga ataaaggaat
    27121 caatacatct ctgctattca tgacaaggag gaatatgatg aaaaaaatag ttggagcgga
    27181 tgaatggata cgcgcagcta gctgcttttt gagatatcaa gtttttgtat tagaacaagg
    27241 gattcctgca gaactagaat tcgacggaaa agatatcccg caacaaacct attatctatt
    27301 tcttgatcaa aaaagaccag tagctacttt gcgttatgaa aagaaaacac ctacttgctt
    27361 caagccggaa cgattttgcg tagcaaaaga ataccgagga aaagggttag gcggtcagtt
    27421 gcttgaagct gctgaaaaac gggcggtttc agaaggttgt cagactagtt atgtagtagc
    27481 tgaaatgacg gctgtagatt tttatgaaaa acaagggtat catacttgca cagaaccttt
    27541 tattgaagat gaagtacttt gtatcggaat gaaaaaaagt ctagcttaaa aacaagagat
    27601 tgtgacacgc cttttgtcac aacctcttgt tttttattta tgtgaatttt tcttattttt
    27661 acgagcagta cggctttgct gtctgcgttc gattttgcgt tcttttttat ttttttctgc
    27721 aattgcccaa tcgatttttt tcttataacc aggcttgatc tttttctttt tcttcttcac
    27781 aagaccaatc agcgtgggat ctaatacatc ttttgatttt tctcgtttgg cacgtctatt
    27841 acgatcgtat gttttgacga tttccccgtt tttgatttct tttggttcaa atgagacgcc
    27901 caatttttct atcgcatcaa tggcttgctc atcatttggc gaatataacg taatggccgt
    27961 tccttttaag ccattacgtc cagtacgtcc gacacgatgg ataaagaagt ctagatcttc
    28021 tggaatttct gcattaataa catgagagac accttcgata tcgatccctc tagcagctaa
    28081 atcagtcgct acaacatatt gatactccag attctgtact tgtcgcatca ctcgttttct
    28141 ctctcttggg gtgatgtctc catgaatctt tgctactttc aagccttggt ttttcaagta
    28201 atcggcaatt tcatccacat gctgttttgt attggcaaat acgattgcta aataaggatg
    28261 accaattgtc agcagttgat aaatcatttg attctcatct ttaccttttg ttgaaatcag
    28321 ccaattttca atgtcttcag aaataactgc ttttggtttg atttcttcaa tgattggatt
    28381 ttccatgtat tttcgtaaga atggacgaag tttttctgga atagtggcag aaaaaactaa
    28441 aaactgcagc ttttctggta atcgacttgc aatttggtct acttcattta aaaaacccat
    28501 atccaatgtc atatccgctt catcgacaac aaatgcaaat gcggtgtgaa tcttcagcgc
    28561 ttgttcattc atcatgtcta atattctacc aggcgtgcct actacaacct gtggctgttg
    28621 gtgatgcaga cgatttaatt gacgctgttt gtctgtaccg ccgacaaaat tcgagacacg
    28681 gatctccggt gtagaaaatt tagcgatttg tgtagctgct tcatagatct gtgcagctag
    28741 ttccctactt ggagctgtaa taacaacttg tacttcctct ttttctgcat tgattttttc
    28801 aaacaaaggc aacaaaaacg tatgtgtttt tcctgtacca gtttgagatt gcccaatgat
    28861 attttttcct tttgcaatta aaggaatcaa acgttcctgg acctctgtgg gctgtttgaa
    28921 gccctttgcg gttaacgctt cattgataaa cggtttgaag cggaatatat caaaattatt
    28981 cattcgtgca cctcttctct ttaaattatc acgactttgg cattataaca cagacaaagc
    29041 gtttttgctc agctaatttc gagaatcatc atcctttgtt tggccaatcg tgctaacagt
    29101 atatgtgtga ctagcttttt cataaaacag aatccgtttt tttctcaaaa atgatagcaa
    29161 gatatttaaa ataaaaatac cttgtagtaa caagacaccg agagaaatga atactgcact
    29221 catctgcaac accgtattcg ttgtcgaatt catgatttgc tgaagcaact gccccaacca
    29281 gcttaaacca taaaaaagaa agtacagata cccgtagcgg atcatcaatg cttttaaaga
    29341 aattcgttcg cggtcttctt ccaccaaacg gatacgcaca atttttttgc caagtgtcca
    29401 tccattggtt ataaacggta caatcataaa gtatacccct acttctaata aaagccacca
    29461 gatatgagaa gtaactgccc ctgaatcgaa cgcaatttta aagaaaatcg ccgctaccaa
    29521 attaataaga gataaaaatg tccagtcgat caaataagcg accaagcgtc gagtcaatga
    29581 gacagtcttt cctttttcat aagaaagttc atccattcgt cttcttgtag gaagcataaa
    29641 tgtaaataac ggcgtaacga caaaaccaat gattccaccc aatgtatttg tcactaaatc
    29701 attcacatca aataagcggt atggtcttgg gtagataaaa taaagtccac ttagctgcgt
    29761 caattcaaaa aatagagaaa ggcagaaact agcaatcatc gttttcataa aagaaagccg
    29821 gaaataatat cttaaataaa tgccaaatgg tatgagtaat aaaacattaa aagctggttc
    29881 caaaaataca ctctgcttca ttgccggcag gtaggtacta ggttcagaca agaccaatac
    29941 cgtgttgtct ctgaaatgtt gccatgaagc aaataaatgc agctccatct ttggccctgt
    30001 catttgggca accgtctcct ttgccggaag tggtaagatc accaaaaaat acgcacacaa
    30061 taaataaaaa acgaaagaat acaaaataac tgcccttgtt actagaaatg ttccatactt
    30121 tcgatattca taaataaaaa aaacagatga gatcgctaag gctaaaaatg gaaaaacaat
    30181 cagcgcaaaa cgaatcggtt cgatataact tgccatatga ctcctccttg attctttgta
    30241 tctctcttat cttagcagtc ctttgctgtt tcaagcaagt aacgaggcta ttatttctca
    30301 tttctttcaa agtaaagtaa gataaacaca aagggggtgt cgagatgctg aatatgtact
    30361 ttgtttttgg cgtacctatt tttttactgt tcctttatgc tacgatcgct tatgtaagaa
    30421 aaagaactac aattcattat ttaggattta ttctcctgat tatttcgggt ttcatgctag
    30481 tcttcaatct tcagacttgg cagcaagcac ttcttgaaat ggataaaatg actccacatg
    30541 ctttgtctaa agtgcttggc taccctgtct atctgatttg gcttcccatt ttcatttctg
    30601 gttgcttggt tttattaaat atttatcgcg gcgtacgccg aatagttcag ttacgcaaat
    30661 caaagtaaaa gacagaaatc ggttgagccc gatttctgtc ttttactttg ctatttcatc
    30721 gtagacaaaa cgacatttac gtgatcaaaa ttaacgagta cttttgacat ttcttccacg
    30781 tactcctcat ttaaataaaa tattttgttt ggcagagaag tgcagttcta ataaaattcg
    30841 aatgttttag ctatctacgt tttaaataga gataaatcgg gataccagca agtgtcaata
    30901 ctatccccat cgaagctaaa aaggtctgag tgataagcgt gcttgatacg ataaaagtcc
    30961 ctccaatgat tgctactagt ggaataaacg gatacattgg aactttgtat ggtcgaaaca
    31021 aatcaggttc ttttttacga agaagaatga caccaacaaa gaccatcgta taaaaaatcc
    31081 aaatgacaaa gatcagcata tccgttagtg tatcaaaacc accgatcatc atcatgccaa
    31141 tagcgatgac tagctctaag atacctgcgg ctacaggaac tttagtttta tcatgaagtt
    31201 tagcaaagag cttgctaaaa ggcaggttgt tttcttttgc cataacataa gggagacgca
    31261 ttccagtaag ggtatagcca ttgatggttc cataaacaga aatcaagata ccaactgtga
    31321 ccagtcttcc accgaagccg ccaaaaatca tttttgcaac gtcacttgcc gcattgctgt
    31381 ttcctgcgac tccgtcgata gatgcggttc ttaagaacac cgcatttact aataaataaa
    31441 cgatcatgat tccgatgatt cctaaagaaa ttgcttttgg caaatctttc gccggctttt
    31501 tcaattctcc cgaaatattt cctacgtgta tccaaccgtc gtatgcgaac atcgttgcta
    31561 gcaagcccgc tcccaaggct gagaaaaatg aaagattttc tcctgcttgg atcggaaaca
    31621 gttggaagtc tactccttct tgtcggaaca aaccgaaaat gacgatcaca aacaaaggaa
    31681 taagcttgca gacaagcgta atcgactgaa aagcccctcc agctttagaa cctaaaaaat
    31741 tgatcaacag gatcgataca gcagctgtaa cagctacggg aacaatcatt gactgggaaa
    31801 gaccaaatag attgacgaac tgtgtaccga aaatgatcga caaggctgct acatttgctg
    31861 gaaaataaat cacgacttgc gcccagccca ataaaaaagc tgctgtattt ccgtaaatcc
    31921 gttcgatata tttaatcatc cctcctgttt ctggaatagc agctgccagt tcagctcctg
    31981 ttagtcctgc acagacagaa atcataccac cgagaaacca agaaaacata tgcaaactgg
    32041 cggatcctgt aacttctgct acgcttgcgg ctttaaaaaa tacccctgca ccgatcactg
    32101 tgcccataac agtcgataaa gcagtaaaaa atcccatcgt acgttttaat tcattttcta
    32161 tttttacttc ttttactggc gtggcttgat attcttccat cccttgtccc ctttcatacc
    32221 atagccaatc ccaaaagtat aaaaaacttt ttggatgtgg ttcttgtttt ataccgtctc
    32281 taccaatttt tctttcagtt tttccaacat atcctttgtc atcacatcta agtcgtattt
    32341 cggcgaaaag ccccattcct cttgcgcaca agaaacatct agtgaatttg gccaagattc
    32401 tgctattgac tgacgtacag gatccacatc ataatccatt tcaaaatctg gtatgatttt
    32461 ttgaatagat gctttgatct cttccgggtc aaaagacatc gccgatatat tgaaggcatt
    32521 tcgatgaacc aatcgatcag gatctgcatt catgagtttt ataatggcat cgatcgcatc
    32581 tggcatatac atcatatcca ttgatgtacc ttgatcaatg aaactagtgt actttccttc
    32641 tttgattgct gaataataaa tatcgactgc gtagtctgtc gtaccgcctc ctggcaaggt
    32701 tgcataagaa atcaatccag ggaaacggac accacgagta tctacgccat atttcacata
    32761 ataataatca catagtagtt ctccagctac cttcgttacc ccatacatgg ttgtaggacg
    32821 ctgaagagta tcttgtgggg tatgatcttt tggagtattt ggaccaaacg cgccgattga
    32881 acttggcgta aaaaatttca gctggaattc tctagcgact tccaatgcat tgaccagccc
    32941 agtcacgttc aaattccagg ctaattgtgg ttttgcttct gcaacagctg acaaaagagc
    33001 agccaaatga atcaatgtgt ccacttgata ttgttctgct atgctgcgca ttttttcgta
    33061 atccattaca tctaggatct caaagatatc gtttttctca atgacttcag gcatgcgtat
    33121 atctgtcgca atgacatttt cactgcctag ttcttgacgt agccgtgtga tcaactctga
    33181 accaatttgt cctaaacatc ctgtcaccaa tacttttttc atgatgatcc tccctgatct
    33241 tttttactac tatcttcaga tgattaacaa tatatgcgtg aacatgttct ctaattttaa
    33301 atagacgaaa aaactaaatt tagttaaata atttccattt cttttcctat tttttgataa
    33361 actgctactg cttcatcaag catttccctc gtatgttccg cagtcggcat atttctgatc
    33421 ctgcctgtcc caagaggcac agttggataa acgattggtt tagcataaat cccatgttcg
    33481 atcagcttct tagagaaagt ctgagtcact ttttcgtctc ccaaaatgac tggagtaata
    33541 ggtgtttcag acataccgat gttatagcct acttttttca attcttcttt aaaataatta
    33601 gcattttccc aaactttttc gacaagttcc ggatgttctt gcatcaaagt aatcgaagcc
    33661 aatgcagctg cagctgcacc tggagttagc gaagtagaaa ataggaatgg ccgacttcta
    33721 gctttcaacc agtcgattag tgttttcgat ccagctacat aacccccaac aacacctata
    33781 gctttcgata acgtgcccat ttgcatatcg attttgtcgc ttaatccaaa atgcttgacg
    33841 gttcctgcac cttttccagt tacgcctgag ccgtgcgcat catcgacgta ggtgatcagt
    33901 ccatattttt caacaatcgg aataatttcc ggcaatcgtg caatgtctcc atccatggag
    33961 aaaacaccat ctgtgatata catgattttt ttatatttct tactttctat cgcttccttt
    34021 gcttttttct caaggtcttt catatcttga tgtttaattc ggatgatttt tgctccggat
    34081 aagcggcaac cgtcaataat agatgcatgg tttaattcat ccgataagat agcatcttct
    34141 tttgtcatta ctgcagagat agcgcccata ttgcagttga atccagattg aaaggcgatt
    34201 gctgcttctg tccctttgaa ttctgcgatt ttttcttcta attgctgatg gattttcagc
    34261 gaaccattga tcgttcggac tgctcctgct cctacaccat aagtttcggt tgcttcaata
    34321 catgcttttt tcaattcttc gcgattggca aatccaagat aattgttgga tgctagattg
    34381 atcatctttt ttccgtctac gatgatacaa gcaccatttt cactttctag tatgtcgatc
    34441 gtattgtata accccttttc tttcagttga tccaattctt tctctaaata cattgttagt
    34501 tctttggaag acattgtctc cacctccata ttcatctgtc ttgcagaaca taccaaacat
    34561 atatcgtgtc attcatctac aaagcaagta tgctatattt ttgataacgc ttacactgat
    34621 tatgagaatt atttaaccac attttttatt tacagtatct ttttactttc aaatgactta
    34681 tatagtatga acaattacct tttagataat gatctatttt actctacctg ggcagataaa
    34741 tacaacacaa ccattgatag aagccctttc gttaaaatca attgtattct gaatatttaa
    34801 tttatttatt gcatagacca ataaaaatac ataaaatttc catcttcgtt tatcagcaat
    34861 ttttaatttt actctctgaa tatttcttaa aagttttact tgttgaattc gaaaaaagga
    34921 ctgtgacaaa agtcttgtca cagtcctatt cttccgaata aacggtgttc tcctgccaat
    34981 tccgaatcac aagttacaat attcatcaaa cacgagaagc tattttttga aattgttcct
    35041 tgtgactcgg agctgaacgg caggctcacg acctcttggt aaacagtgtt ccagaagtaa
    35101 cttttacgga aaatgatccg aaatatcttg gagctgacca cttctgtcac aacctccatc
    35161 attttttgtt atttttgatc caattgtctg gagtaaaatt ttcatcgtca atagaccatt
    35221 ccagaaaaaa ttcttctaag aattcacgca tgaaggtttc tcttcgtttg gcttcttcat
    35281 atccgtaatc agtattcatt ttatcagaca gcaagaacag tttttcataa aaatgattga
    35341 tcactgtcgt gccttttcgg taattttctt tgtccttgaa tgtttgcgga tacagttcag
    35401 gatcaaaaat cgggtgccca tgtccaccgc cgaaataagc agtacgcaaa atgccgattg
    35461 ctcctaatgc ttcgatacga tcagcgtctt gaacgatctt accttccaga gatagttcgg
    35521 ccgcttcaga gagattttta gaaaaggaca tattttcgat aatggaaaaa atcatcgaga
    35581 tctgttcctc tactattggc aaagagcgaa ggaatacacg aagctgttgc tttgcctcgt
    35641 tctcattttt aacgacttta tcatcaatcg tatcatgtag gtaagctgct gcaagcgtca
    35701 caaagcggtc tgcctgcggt tccgtctcca aaattttttc agctaacttg acaactcttt
    35761 tggtatgatc gctcccatgt cctgtttgat catgcgctaa ttggtccata ctataatcag
    35821 cgatcacttg taatttatcc attcgttcca cttctttctt aagtaaatag ctgaaaacat
    35881 ctatagatta gatgaatttc agctatcttt gtaaattgta ttttatttta cctgatatac
    35941 attaaaatac aggtctttca tatagtcgat caggtattgc ggattaagcg gttcatgtgt
    36001 tgcatccatg attaattgat ttggttttct agatgcacca tattgatgga tatgctctga
    36061 catccagttt ttgattggag aataatcgtc tgacgctaga acattttcca catctatttc
    36121 ttgctgcaaa gaatgaagta attgtgccgc atacatgtac cctaatgcat aagagggaaa
    36181 ataaccaaag cttgcacctg accaatgaac gtcttgtagg atgccttcca catcatttgt
    36241 tggtcgtatt cccagatact cttcatattt ttgattccat atttcaggta gttcagcaac
    36301 atctacccct tcattgaaaa tcattttttc gatttcataa cgaatgatga tatggagagg
    36361 gtaagtcaaa ctgtctgcct cgatacgaac caaacttgat tttgtatggt tcaatgaacg
    36421 ataaaatttg tcaaaaggaa tatcatcgaa tgttccttct gcacattctt gaaagaaagg
    36481 atattgcttt ttccagaagc tgcgactact tccaataatg atctcgttaa acaacgattg
    36541 tgattcatgg atacccattg atgtaccttc ataaaccggt gtatacgcat atttatcatc
    36601 gatgtcttgt tcatacatac catgaccagc ctcgtggatc actccaaaaa cagccatttt
    36661 gaaatctgtt tcgttccatc gtgtcgtgat tcgtgcatca ttcggattaa ttccagtcat
    36721 aaatgggtgg acagtgtcat ctaaacgacc acgactaaaa tcataaccaa gttgttcgat
    36781 tactttcact acaaatctct tctgttgttt tttcgtcatt ctccgattta aaaagtctgt
    36841 ttcaggttca gtcccttttt cttggagcgt acggcggatc gaaagaattc cttctttaag
    36901 ctgatcaaat accttatcaa ggatttcgac agtcatcccg ggttcgtatt gattcagcaa
    36961 aacatcataa ttcgtctttt catcttttct ccagtaaggt atcaactgtt ttgttaattc
    37021 aatattttga ttcaatgcgt gttgaaaatc agaaaattgt tgtgtttctc tagctttttg
    37081 ccattgtccg tgtgctgaag aaacggcagc tgtatatttt ctcataagat cttccggtac
    37141 gcgatgttcc aaatcatact cttcttttac tttggcaaaa acggcagttc caacttccga
    37201 caattcttcc ggatgttcag aaaaatagcg aatggcttct ttgatttttg gaccgatttt
    37261 ttttgaaaaa taaatgctgt aaagatagct gtctacttca cttcggtatg cactagcttt
    37321 ttcaggcata cctgtttgtg tatcccaatc gaggattcct aaagcttgtt gtagaagatt
    37381 gatttctttt acttcttgta aaaactcttt ttctttcatt ccctattcct ccaaacatcc
    37441 aattgaatga atcaattagt aagattattc gtctttctct ttttttctag gcggacgagt
    37501 tccccaatac tggaatagat ccgtacgcaa tgcaccattg tacaattttc tttttttggt
    37561 tgctttttgt ccgtaaaatt cctcaaacgc taagtcgcta gtcaagatat atttactcca
    37621 tgtttttaat ggacggaata catcgcccat ttctttatat aatttccgaa cagtttcttc
    37681 ttctcctaga cgttctccgt aaggcggatt agccacgatg accccgtatt ctttatccgt
    37741 tttgaagtct ttgactgctt gttgcttaaa ggtaatcgaa gagcctaatc cgatttcttc
    37801 tgcattggct ttggcaattt caatcatccg tccattgata tctgaaccag taatatctaa
    37861 ttcaatatca taatctgctt tttcttcggc tgctgatcga acattttcca tgatttcttg
    37921 actgaaccaa tcccaagatt cgcaagcaaa ctcacgattg aatcctggtg cgatattatg
    37981 gcctattaag gctgcttcaa tacaaagcgt acctgaacca catacaggat cataaaatgg
    38041 acggtctttt cgccaatttg tcaacataat aagtgctgct gccatgtttt ctttcaatgg
    38101 tgctccacct ttttctaagc gataaccacg tttgaataaa cttggtcctg tcgtatccag
    38161 agttaccatc acatggtctt tcagcaaagc tacttccaac tgaaaatgtg cgccagtttc
    38221 cgtcagtggt acagaagctg gacggtgata ataggtacgg agacgttcaa cgattgcttt
    38281 ttttgtgatc gcttggcaat caggtgtgct atatagtttc gacttgatcg attttccagc
    38341 tacaggaaaa gctgcatcta gtggaagaaa atcttcccaa ggcaatgctt ttacgttttc
    38401 aaacaattca tcaaacgtat acgcatcgaa ttccccaacc acgattttta ctcgatctgc
    38461 tgtacgtaac cataaattag ctgtagcgat cgtttccatt gttccttcaa aacgtgcacg
    38521 tccgttctca acttggcatt cgattccaag atctcttaat tctttaccaa ccagtgcttc
    38581 tagcccgctt gccgcagttg cgacgaggtt gaacttcctg tttttttcca tacacaatct
    38641 ccttatttat aaaaaa
//
LOCUS       DAFBQH010000021        38600 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.021, whole genome shotgun sequence.
ACCESSION   DAFBQH010000021 DAFBQH010000000
VERSION     DAFBQH010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.021"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(99..1229)
                     /locus_tag="LK694_001106"
     CDS             complement(99..1229)
                     /locus_tag="LK694_001106"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBK5051992.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDRLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="LK694_001107"
     CDS             complement(1878..3080)
                     /locus_tag="LK694_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK5051993.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="LK694_001108"
     CDS             complement(3140..3343)
                     /locus_tag="LK694_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HBK5051994.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="LK694_001109"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="LK694_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="LK694_001110"
     CDS             4880..5692
                     /locus_tag="LK694_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5051995.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6913)
                     /locus_tag="LK694_001111"
     CDS             complement(6716..6913)
                     /locus_tag="LK694_001111"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBK5051996.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="LK694_001112"
     CDS             complement(7059..7937)
                     /locus_tag="LK694_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK5051997.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="LK694_001113"
     CDS             complement(7976..9094)
                     /locus_tag="LK694_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBK5051998.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="LK694_001114"
     CDS             complement(9091..11190)
                     /locus_tag="LK694_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBK5051999.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="LK694_001115"
     CDS             complement(11241..12062)
                     /locus_tag="LK694_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK5052000.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="LK694_001116"
     CDS             complement(12059..12868)
                     /locus_tag="LK694_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5052001.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="LK694_001117"
     CDS             complement(12889..13383)
                     /locus_tag="LK694_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5052002.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="LK694_001118"
     CDS             complement(13395..13883)
                     /locus_tag="LK694_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5052003.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13960..16656)
                     /locus_tag="LK694_001119"
     CDS             complement(13960..16656)
                     /locus_tag="LK694_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK5052004.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="LK694_001120"
     CDS             complement(17084..18157)
                     /locus_tag="LK694_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK5052005.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="LK694_001121"
     CDS             complement(18154..18981)
                     /locus_tag="LK694_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5052006.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="LK694_001122"
     CDS             complement(18982..19788)
                     /locus_tag="LK694_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5052007.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="LK694_001123"
     CDS             complement(19800..20885)
                     /locus_tag="LK694_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5052008.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="LK694_001124"
     CDS             complement(20902..21444)
                     /locus_tag="LK694_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5052009.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="LK694_001125"
     CDS             21702..22691
                     /locus_tag="LK694_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBK5052010.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="LK694_001126"
     CDS             22688..23425
                     /locus_tag="LK694_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBK5052011.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="LK694_001127"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="LK694_001127"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBK5052012.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTVIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="LK694_001128"
     CDS             23989..24621
                     /locus_tag="LK694_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK5052013.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="LK694_001129"
     CDS             24706..25353
                     /locus_tag="LK694_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK5052014.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="LK694_001130"
     CDS             25358..26341
                     /locus_tag="LK694_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HBK5052015.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(26744..28258)
                     /locus_tag="LK694_001131"
     CDS             complement(26744..28258)
                     /locus_tag="LK694_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBK5052016.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            28396..29202
                     /locus_tag="LK694_001132"
     CDS             28396..29202
                     /locus_tag="LK694_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052017.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(29377..29946)
                     /locus_tag="LK694_001133"
     CDS             complement(29377..29946)
                     /locus_tag="LK694_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5052018.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(30029..32089)
                     /locus_tag="LK694_001134"
     CDS             complement(30029..32089)
                     /locus_tag="LK694_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HBK5052019.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            complement(32366..33844)
                     /locus_tag="LK694_001135"
     CDS             complement(32366..33844)
                     /locus_tag="LK694_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5052020.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(34142..35077)
                     /gene="murB"
                     /locus_tag="LK694_001136"
     CDS             complement(34142..35077)
                     /gene="murB"
                     /locus_tag="LK694_001136"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBK5052021.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(35150..36070)
                     /locus_tag="LK694_001137"
     CDS             complement(35150..36070)
                     /locus_tag="LK694_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK5052022.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            36183..36935
                     /gene="xth"
                     /locus_tag="LK694_001138"
     CDS             36183..36935
                     /gene="xth"
                     /locus_tag="LK694_001138"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBK5052023.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            37799..38332
                     /locus_tag="LK694_001139"
     CDS             37799..38332
                     /locus_tag="LK694_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBK5052024.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT        11575 a         7961 c         6544 g        12520 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ctatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttgccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacggt catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    26521 tcccacgctc tttttccgaa taaacggtgt tcaaaagctt gcatctgcgg taataagtcc
    26581 aaccaagtta aaaacataag aagctgtttt tacttggctg ttcttattac ttgatgcagg
    26641 gcagcttttt cacaacctct tcactaaaca gtgttcaatc agctgaccct cggtattagc
    26701 tcaaaacgct gatttcacaa cctcttttta tttacgttct atttcattca ttttccgaag
    26761 caaatcgagc tgcttgtatg cccgcttgtc gcccaaaaat aatgatctcc gcaactgaat
    26821 ttccaccgat ccggttgttt ccatgtaagc cacctgtgac ttcaccagca gcatataacc
    26881 ccctaattgg ctggttttct ttatctaata cttcagtgtt cgtattgatt ttcacaccgc
    26941 ccatcgtgta atggattcct ggtgcgattt tgatcgcata ataattcgga gacgataagt
    27001 catgttccat ggctgtggca cggttgaatt gagcatcttt tttctccgca acatccgtat
    27061 tccatccatc aactgttttt gctagtgttt cttttggtaa atcgattttt tctgctaatt
    27121 cttccattgt ctttccagtt gtgacatatc ctttttggtc ataaaattca atagctgtgg
    27181 cacgatcacg cacgccttgg tcaaaaatca aataagctga tttttcaggc aatgctgtaa
    27241 ttgctgcaga tactttatcc cttgtatcca tttcattgac aaatcttttc ccctcttgat
    27301 cgaccaagat tgctccttct ccgcgaacaa cctcaccaat caagaccccc tcatcctgct
    27361 gcacagttgg atgaatttgt attttatcca tatcaacggt ttgtccacct aatttttcaa
    27421 tcatggtgat accatctcca gttgatcctt cttgatttgt cgtaatataa tcagctaaat
    27481 cgggacgata tttttcaata aattctttgc tggcaccaaa accgcctgtg gtaactacga
    27541 ttgcttttcc ggtgacctct tttggttctt ctccttgaac atgtacagtc acttcattga
    27601 ccgtttcccc atttttctta atgtctgtca catctgcgtt gacaaaaata gggatttccc
    27661 gctcatggac gttattcaat aacccctcca ctaagtatcc accaatagcc gagccatcag
    27721 acggacgatg tgtcctcttt tcactcatcc ctccagtgat cgttaagttg tccagtttga
    27781 taccgttttg atctagccaa tcgaccgcat cagcagagtg atcgacaaag tatcgaagca
    27841 tttctttatc attcgttcct ccaccacctt ttagcgtttc ttcatagaag gcatcattcg
    27901 agtctttgat tccttcttct ttttgaaatt ttgtttctga agcattcata ccgcttgatg
    27961 atttagatgt gtttcctccg gcaacaggca tcttttccaa aattactgga ttcaacccag
    28021 catcttttgc ttcgatagct gctgccatcc ctgcaccacc agctccaata atcacgacat
    28081 cataagattc tttcatttct tttggatctg tatatacttg ttcagaagcg cctgatgata
    28141 cttcggcaga ttccttcgta ctagaacttg tctgcgctgt atttttctga tctgcgccgc
    28201 atgcgctcat agcaagaagt gcaacggctg ttaataaacc agcaactaat tttttcattt
    28261 gtatccccct tttctcctta tactatatta agtgattttt ttcacaatat caactctgtt
    28321 tttctttaag tcatatagct ttggtaaaca cacgcttctt agtataatta aggatataca
    28381 tgaaggagtt attttatgaa tcatttgaaa acctttttta cgaacagtac aaaaactgca
    28441 ggtagaatca caaacggaat ctatatttta ttacgtatca tcgtcgttat tatgctagtg
    28501 gatcaggtct ggaaccaaca ctggggaaat acaatgcttt tattactgac attaatttta
    28561 tttgaaatcc cttattttct ggaaaagata ttaaagatac agattccaaa tatgctagaa
    28621 atcattttgt tatcttttat ttttagttca acagttttag gagagttaag tgatttttat
    28681 ggctattttt caatgtggga tacttttcta catgcattca gcggattttt agctgggggt
    28741 gtcggctttt cccttgttta tttgctcaat aaaaatacaa aaaccatgaa cttgactcct
    28801 ttattactag cagttgtttc cttctgtttt tcgatgacag ttggtgttat gtgggagttt
    28861 gtggaatttt cagcagataa attactgcaa ttcgatatgc aaaaagatac gatcatccag
    28921 caaatcaata gtgtggagtt aagtaaagac ggaaacactg tctatcatgt tccagatatc
    28981 actcggacaa tcattgaaag tcaaaatgca gatggtaaaa cagataaaac aatcatccaa
    29041 aatggttact tagacgtggg aatcatggat actatgaagg atttatttgt taattttatt
    29101 ggagcagttg ttttcagtgt atttggttac ctctatagtc gaaacgatta ccaaaaattt
    29161 acgttcatca agaattttat tcctaaaaaa atcaaacgat aaaacatgct aaaaaacaag
    29221 agaaggggca gtgacaatac ttttgtcact gccccttctt ttttgaatgt atggcatgcc
    29281 attagttatc cgatagtatt aactcaaaaa agtaagatat gaggaatgac tatttcgttt
    29341 tttatcccaa tactgaggac ttatttcacc atttcgttat ttatctaatt tacttaaaac
    29401 ctgcataact ttttcataat ctggattagg tgtgccgtct agattttcag caataaaagt
    29461 aaaacccatt tttttataaa gagcaatcgc ctgatggctc catgtctgtg tgtgaagata
    29521 gattggtccg tccgtatgga gggtaggaaa ttcttgcaag actctggcag tcaatgcttt
    29581 tgcgagccct tttccttggt aatctggttt cactgctaac caatgaaaaa ccggatacac
    29641 ttgctctctt gatttttttc gccaagcggt acaggttgct actttttctc ctttttttgt
    29701 ctctacaaaa aacatccttc tttcaagttc ttcaggataa ggagcaaagt tttgttgaaa
    29761 atactcaagt gcttcatctg tcgtttgaaa ttccccaacg gaagtctcaa tctccgccca
    29821 agcagattca tcttcttttt gataaaatcg aaattcaaaa ccttccgcta aaggcgtgat
    29881 tggaacattt tgttgaagtg gtcttgtcat ccagatttct gcgtaaggta acgttttatc
    29941 taacattttt ctatccctcc tcgtctccat tatgccaaat aacatatgac tcagctactt
    30001 atttgactat aacagataga gatataaatc agaaccagtc atctgcgtca aattcatttc
    30061 gattagcgta tacccatgtt cctgccatac ctcgcatgaa atctcctaaa gcagaagaag
    30121 gaatacctcc accaggtgcg ggtgttcccg gactaggaag tggtacttgc ggctgcagat
    30181 gctcatcggt ggaatcaata gttgacaagc gttttttctt cgtttccttc tggtcatctg
    30241 attccttctg atcttccgac tgtttagcaa caaattcttt ttctgattgt tgttttattt
    30301 taggctctaa aatttcattt tccaatccag aaacttgtgg ggccaaatga gcttcttgcc
    30361 ttatcgtttt attagattct gagatttttc tagatattct tgctaactct aggcttcttg
    30421 tttgttttac tttccctgtt ggagaaactg catgagccat ttgttgtttt ttttctgaca
    30481 agctttcttc ttgtcttttt tgttcttttt gatctatctg tttgtttaat ggtaaaacat
    30541 gtaatagtga ctgagcatgg agttcttgga gtgaatacgg gtcatgataa ctatacataa
    30601 tcgtaaaatc acctgtttgt ttggtcaaat aaccatcctc caatggtact tcattgattg
    30661 taaccgatac atcctttaac ggtacgggtt tacctaagga atttgttgct gattggatca
    30721 actcttcttt gtttattttt tcgtttggat gaacaataag atcatgacct tctaatgaac
    30781 gattgtctgg tatataaaca taagtaaagg taatttcccc tcctgattgc ttcctattat
    30841 tcatcagttc tgagtgaagt acttcaagga tctccgacca gtgacttttt ctgtctaatc
    30901 cgtttgcttg catccaatca gaaatagaag tgactttccc attttgttca taagtcgata
    30961 aatctatatt tggcactcga tcagaaaatt cagcaggtag gcgcaaatca ggaatacgat
    31021 ttacactact catatgtgtt tctgcttgaa gttctttatt tgtttgacta tctataaaat
    31081 gagtgatgac agtcgatgaa gcagcagatg cttctacata aactacagct gttgtttctg
    31141 ccacatttga tgttttcgag tctatataag ttaatacaac gggatattct cccggagttg
    31201 tagtatctac tgtcccgttc attttgattt cttctttatt tcctgaagtt ccatcgcttt
    31261 ttgttacata ttcaacaaga tctttagggg cgtaactctc tcctgttctt aagtgaagat
    31321 cttttccttt gatagctgtt tgatcagcac gaacatggat cacagcagat gcagtcattg
    31381 tttttaaagc aattggatca aaataggaca aggaaatagt agatgttcct gtattagtcg
    31441 tatccacgat attcccattg tttgaaatta ctttggacat atctccttgt gttccatctg
    31501 cgttcttcag cctttctacc agatcagata tcgaaacttt tgtccctgcc agaacatcga
    31561 ttttctttct ttcaaactgt gaataatctt tttcatatac ataagaaact tgtgcgcttc
    31621 caccgtcctt tatatctgtc aaagcatgat tcaaaatatc agtgatctgt tttaactgat
    31681 caccttttgt aggatcagtc atatcggaaa agatagaaag cggcacactg cttccggttt
    31741 ccgatgtcca taatgttttg ttaggaatta accagtagtg gtttatcgtt ggaggataac
    31801 tgactttatt ataggaagtc aatgcaggaa gtagctgttt tccatttgca tcagtgaaag
    31861 tatgagtaat agtgataggg gaatcagtgg tctcagaaac agaggtacgt acgaccttga
    31921 tggtttgtgt cgttgcctga acgggtacat gcatatagac acctacagat aaactgatca
    31981 gcatcgtgcg catcataaac ttaacgtagt gtttttcaga acacgaccgt tccttatcta
    32041 aacgacaagc gtgtttattt agttgttttg aacgtctttt tgaacacatt tattcttagt
    32101 cctccctact cttgtctaaa catgaagtag taattttcat aagatgattt cattatataa
    32161 ataaaatcaa aagaagatgg actattagga gagtattttt tttgattttt tattgtttca
    32221 agaaaatcgg aataataact tatgtacttt ttaattttgt taagtaatat atctagttat
    32281 atctagttat atctagttac cttttctttg aaaaaaacat aaatcagcta ataaatatgt
    32341 gagacaaagc tgccatccat aaatactagc tgattttttc agttaaatgt ctattttttt
    32401 ctctgatgat ggtatttcca aaatattccc agtcatctgg agtcggtgga tttttccaaa
    32461 ttatctgttc tttattaaat ttttcaagta aacaatcaga taaataaata ttcgtacgag
    32521 aagtgattcg actctcaatc acaatattgt aatttttaaa tcctttcact tgagaaataa
    32581 tgtaatcggt ataagcagat ccttgcgaaa aatcaataca gacatgaatc ggtgcttcaa
    32641 aatatgatac cggacaaata ccgaccaata aaaagagata atcataaaat aagcgaatca
    32701 tgaaacgttc atcttttccc aagaaatagc gtttattctc ttgtatgacc ttttggattg
    32761 ctgtactgaa tattggatac gtctcgaaga aaaactgtag ctgcttttta gacgtaaagc
    32821 tagtcatctc aaagtcgaat atccaatttt ttcgattgac atgcatcata cccatacgaa
    32881 gttcttttcg tatatatagt ctttcttctt cagagatttc aatcgaaaat ggtaattgat
    32941 caatcaattc cactgacatt ttttgactaa tcatgtggat atcatgaaaa tgagcttccg
    33001 caagtccaaa tgcgccatct aattcaaatt cttcttccgt gatcaaaaac aacatgatat
    33061 aagaaatctc atcaatgatc tgctctttag ggatatgaag aaaagaaata tgtgataaat
    33121 ccttgatgat cttcattcct tctgagctct taaagtattc atctgaaaag aaacgttttc
    33181 cgattggttg attcccatag gccctcaaca aactaatagt ggttagcaaa ttgaatttga
    33241 ttcgttttgt tgcagacagt tttaaattga acagatattc aaaatattgc acgatactct
    33301 ttgcttcatt catgacgctt tctctaaatg gcatagaaaa accattaaag accccgaaat
    33361 ataccccaaa taatacgttc cgtatgttta tttcttcccc aactaatatg tttttcttta
    33421 actttatttg atgctgttca agaaaagaga ctaattctct tctttttaca taggctcttg
    33481 atatgcttaa aaaatgattc tctgcaaatt tttcgatcgt tacttttgaa tccacgatct
    33541 ccgttaacag tcgagcaatt tttgatcgtt gaaagtagat tcttcttaaa tttttgatat
    33601 ccgtcaaatt cagacgttcg acatggatct ctgcttcttc tgaaatttgg atctctggct
    33661 ttgttttgaa ttcagtaaat tctttattta attctatgat ataatttctt gttttaaatt
    33721 tagacaaacc taatgtttca tataacttag gcagcactac ataattcttt ttctcaaatt
    33781 ctaaatatct agctagcagc agtttttctc tatcttcctc ttctataagg taatccaact
    33841 tcatatccta cacctcaaaa aatgcctcat accaattact tgatatgcag atgaaagttc
    33901 atcttttcac ctatactgat tttatgatgg attatgcttg ttttttcatc agttattctc
    33961 attttttatc ttttttttga gaatattgca aaaaatggaa aaatttatga ggaaatattc
    34021 tatatttttt gttttattgt gttaaaatgt acaagatttg cgccatttgc gtaaacaacc
    34081 acaaaaaaga gcaggacggc caccgtcctg ctcttttact tattttaagt tccattactt
    34141 attaattggc taaacttctg cttgtttctg atattgcttg tttttctcca atgatccgca
    34201 cttctgtttc taaagacaca tcaaattttt ctttgattac ttcttggatg tgcgcaatca
    34261 attcaatata atctgtcgct gtcgcatgat cgatattgac gatgaatcca gcatgttttt
    34321 cagagacttg tgcaccgccc catttcaggc cttgcaatcc tgcttgttgg atcagctgac
    34381 ctgtatagtg tcctactggg cgcttaaaga cacttccgca tgaagggtat tctaatggct
    34441 gttttgattg acgcaattcg gtcaactcat ccattcttgc tttgattgct tcataatccc
    34501 ctgtttgcaa actgaatcga gcttccaata cgatagcacg taattcttgg actttactgt
    34561 gacgataaga aaataccatt tcgtcttttg tcaaagtttt caatgttcca tcttcaagca
    34621 aaagatccac ttcagcaaaa acatctttga tctctccgcc atacgcacct gcattcatat
    34681 aaaccgctcc accgacactt cccgggatgc cgcaagcaaa ttcaaatcca gtcaatgatt
    34741 ctgccaaagc tgcataagtc gtatcaatca gtgaggcacc tgcttccgca atgaccatcg
    34801 ttcctttgac atgaatctgt ttcatttcag tcagcatgat gactacaccg cgaatcccgc
    34861 catcttgaac gatcagattg cttgcatttc ctaataccat ccaaggtata tcatggattc
    34921 ggcaataatc gatgatttgc tttacttctt cttgtttttt cgggaatgca aggacatcag
    34981 ctggtccacc tgttttcgta aacgtatagt tcatcaatgg ttcatcaaac aatatagtaa
    35041 tcgtggctaa gctttcaact atttcttctt tattcactga ggattttcct ttcttttctc
    35101 aaacgtcctc attattctat cacgcttaat acaatcttgc cagtcttgtt caaagttgat
    35161 gacggtagtg atctttaagc acttcctcac ataattcatg acttcttaca acttcttttt
    35221 gtttaagcaa ttctgtttcc gatttaccta aacggatctg agaaaagaag gcctctacca
    35281 ttggctcaaa cccgcgtttg actagcgtgt ttgtccagtc gccaaattct ttcacttttt
    35341 ttccatcagc atcttgaatg acaagctggg tcagatcgtt tacttcataa gtaccatttt
    35401 tacttgttac ttgatatttt tctatattag cgccacttgc taaatccatc gttaaaatag
    35461 ccgtttgatt ttgtgtttct aattgtaaaa tggcatattc caaaaattcc tttgtttctc
    35521 ttatctggta ttttgttctc aaaatgggtt catctaataa atacacagct gtatcaataa
    35581 gatgtaaaaa caaatcataa aggacgaact ctgtcgtttc ttttgccgct attcgatttt
    35641 tttgcaggtg gatcaaccgc ttatctggga tacgtttcaa ttcttctacc ataggagcaa
    35701 agcgccggtt gaaaccgatc atcaaaatta tttggttctt atcagctaat tcttgcaatt
    35761 cacgaacttc atttagatgg acactcagtg gtttgtcaat ataaacatgg ataccatttt
    35821 ctagacattt tttggctaat tcataatgta cttttgttgc tgcgtggatc atacatgctt
    35881 cgattttttc ttctaaaagc tcatcgatcg tttcatatag atgttggaac ccgtactctt
    35941 tttgaattct ttcttttgtt tctttatttc ttgttgcaaa ataaaaatca agtgttcctt
    36001 gttttttcgc ataagttggt aaatatgctt tttctgcgat attccctaca ccgattacac
    36061 caatcttcat ctactttcct cctggatcat ttagttactt tcatcttaca ccatttttta
    36121 ctaaaatgag gtacaatagt atcgcaaagg taaggaaatt aattcgaagg agtgtgcttt
    36181 tattgaaatt tatctcttgg aacgtaaacg ggcttcgtgc catcgtaaat aaaaattttt
    36241 tagaagtttt tcatgagtta gacgccgatt ttttctgtct gcaggaaacg aaactccaag
    36301 caggacaaat tgatttagat cttccaggtt attatcaata ttggaattac gcggaacgaa
    36361 aaggctattc cggcacagcg atttttgcta aaaaaccagc attgaatgcc acttacggaa
    36421 tgggtatcga tatccatgat acagaaggcc gtctgattac tttggaatat tccgatttct
    36481 ttcttgtaac ctgttacacg ccaaattctc aaagcgaatt aaaacgttta gattaccgat
    36541 tagaatggga agaagccttt tataattatt tggaaaatct aaaaaaacaa aaaccagtca
    36601 tcgtgtgtgg ggatctaaat gtcgctcatc aaaagattga tttgaaaaac tggaaaacga
    36661 atcaaaaaaa tgctggattc actccagaag aaagagcggc tctttctcga ctgttggata
    36721 acgggtttat cgatacattc cgttattttt atccaaccca ggaaggtgtc tactcttggt
    36781 ggaactaccg attcaattca aggaaaaaca atgctggttg gcggatcgat tactttttga
    36841 cgagcaaaga tttagagcca ggactagctg atgcaaagat ccatacggac attatgggca
    36901 gtgatcattg tccagtagaa ttagatttga aataaagagg ttgtgaaaaa gtcgtttagt
    36961 ttcgagtatt aagaaaaatt ttgtgaaaat ggctttccac attttgcata aatttggcat
    37021 aataccgaga aactggcttt tgaacactgt ttatcgagag attgtgaaaa agctgtcctg
    37081 catcaagtaa taagaacagc caaacaaaaa tagttcctca tatttttcgt ttggttgggc
    37141 ttattaccgc agatacaagc ttttgaacac cgtttattaa gaggttgtga aaaagctgca
    37201 ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatattttt cgtttggttg
    37261 ggcttattac cgcagatgca agcttttgaa caccgtttat taagaggttg tgaaaaagct
    37321 gcactgcatc aagtaataag aacagccaaa caaaaatagt tcctcatatt tttcgtttgg
    37381 ttggacttat taccgcagat gcaagctttt gaacaccgtt tattgagaag ttgtgaaatc
    37441 agcgtcctga cctgagtatt agagaaacaa atggcaaaat agttcctcat attttgctat
    37501 tttttgagct aataccgagg gtcagctgat tgaacaccgt ttagtggaga agttgtgaaa
    37561 aagtcgccta ctttcgagtc gtaagaaatt tttttgaaaa tggttcctca caactttgca
    37621 taaatttgac ttaataccga gaagtcatga tccccatcta actccaaatc acaaggaatg
    37681 agttcaaaaa ctagcttttc tcaacaaata ttacataaaa aattctatgt tttttgttaa
    37741 atttatttgc ttttttcaaa agtatgttac agtaatttcc caaggaggtt ttacagaaat
    37801 gaattttgtt gccatggatt ttgaaactgc caatcatcag ccttatagtg cttgttctct
    37861 tgcgctagtc atggtaaaaa atagccagat cgttgatgaa ttctatacat tgatacagcc
    37921 tgagactcct tttttctggc gaaatgtcca aattcacgga atccatcaag aagacgtacg
    37981 caatgcgccg aaattcccag aagtctggca aactatccaa ccatattttc aaaaaaatcg
    38041 tttagtcgtt gcacataatg ctgcttttga tacaaaggtc ttagcgggat gcttagatta
    38101 ttatcatctt gaacagccta attatctttc tttatgtaca gtgaaaagta gccgtcgcct
    38161 gtttcctgaa tttccaaatc atcgattgaa cactgtgtgc gaaaatttga acatccaatt
    38221 agatcatcac catgatgcat tggaagatag tcgtgcatgt gcggaaatcc tgctttatca
    38281 agaaaaaaaa ttcgggacag atccattgaa aaaacttgtg actataaagt aaaagaatac
    38341 ttatgaaaca cacaaagagg tcgtgagcct gccgtttcac tccgagtcac aaggagcaat
    38401 ttcaaaaaat agcttctcat gtttattaaa tattgtgact ggtgaccgag gagctggcag
    38461 gagaacaccg tttattggag aggttgtgaa aaagctgttc tgcatcaagt aataagaaca
    38521 gccaaacaaa aatagcttct catatttttc atttggttgg acttattacc gcagatgcaa
    38581 gcttttggac accgtttacc
//
LOCUS       DAFBQH010000022        38334 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.022, whole genome shotgun sequence.
ACCESSION   DAFBQH010000022 DAFBQH010000000
VERSION     DAFBQH010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.022"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            161..391
                     /locus_tag="LK694_001140"
     CDS             161..391
                     /locus_tag="LK694_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBK5052025.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="LK694_001141"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="LK694_001141"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBK5052026.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="LK694_001142"
     CDS             1802..1978
                     /locus_tag="LK694_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBK5052027.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="LK694_001143"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="LK694_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBK5052028.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2593..3060
                     /locus_tag="LK694_001144"
     CDS             2593..3060
                     /locus_tag="LK694_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5052029.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3145..4029
                     /locus_tag="LK694_001145"
     CDS             3145..4029
                     /locus_tag="LK694_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK5052030.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4143..4379
                     /locus_tag="LK694_001146"
     CDS             4143..4379
                     /locus_tag="LK694_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBK5052031.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4467..4547
                     /locus_tag="LK694_001147"
     tRNA            4467..4547
                     /locus_tag="LK694_001147"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4501..4503,aa:Tyr,seq:gta)
     gene            4554..4625
                     /locus_tag="LK694_001148"
     tRNA            4554..4625
                     /locus_tag="LK694_001148"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4586..4588,aa:Gln,seq:ttg)
     gene            4750..4920
                     /locus_tag="LK694_001149"
     CDS             4750..4920
                     /locus_tag="LK694_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052032.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5140..5847
                     /locus_tag="LK694_001150"
     CDS             5140..5847
                     /locus_tag="LK694_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5052033.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5907..6551)
                     /locus_tag="LK694_001151"
     CDS             complement(5907..6551)
                     /locus_tag="LK694_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBK5052034.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6678..6965
                     /locus_tag="LK694_001152"
     CDS             6678..6965
                     /locus_tag="LK694_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052035.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7073..7393
                     /locus_tag="LK694_001153"
     CDS             7073..7393
                     /locus_tag="LK694_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5052036.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7474..8787
                     /locus_tag="LK694_001154"
     CDS             7474..8787
                     /locus_tag="LK694_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBK5052037.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8784..9503
                     /locus_tag="LK694_001155"
     CDS             8784..9503
                     /locus_tag="LK694_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBK5052038.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9631..10182
                     /gene="raiA"
                     /locus_tag="LK694_001156"
     CDS             9631..10182
                     /gene="raiA"
                     /locus_tag="LK694_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBK5052039.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10404..12938
                     /gene="secA"
                     /locus_tag="LK694_001157"
     CDS             10404..12938
                     /gene="secA"
                     /locus_tag="LK694_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBK5052040.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13064..14165
                     /gene="prfB"
                     /locus_tag="LK694_001158"
     CDS             join(13064..13135,13137..14165)
                     /gene="prfB"
                     /locus_tag="LK694_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBK5052041.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14270..14956
                     /gene="ftsE"
                     /locus_tag="LK694_001159"
     CDS             14270..14956
                     /gene="ftsE"
                     /locus_tag="LK694_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBK5052042.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14940..15833
                     /locus_tag="LK694_001160"
     CDS             14940..15833
                     /locus_tag="LK694_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5052043.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15985..17433
                     /gene="cls"
                     /locus_tag="LK694_001161"
     CDS             15985..17433
                     /gene="cls"
                     /locus_tag="LK694_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBK5052044.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGGKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17770..18627
                     /locus_tag="LK694_001162"
     CDS             17770..18627
                     /locus_tag="LK694_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBK5052045.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18742..19662
                     /gene="pstC"
                     /locus_tag="LK694_001163"
     CDS             18742..19662
                     /gene="pstC"
                     /locus_tag="LK694_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBK5052046.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19662..20546
                     /gene="pstA"
                     /locus_tag="LK694_001164"
     CDS             19662..20546
                     /gene="pstA"
                     /locus_tag="LK694_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBK5052047.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20561..21367
                     /gene="pstB"
                     /locus_tag="LK694_001165"
     CDS             20561..21367
                     /gene="pstB"
                     /locus_tag="LK694_001165"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBK5052048.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21383..22141
                     /locus_tag="LK694_001166"
     CDS             21383..22141
                     /locus_tag="LK694_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBK5052049.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22154..22831
                     /gene="phoU"
                     /locus_tag="LK694_001167"
     CDS             22154..22831
                     /gene="phoU"
                     /locus_tag="LK694_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBK5052050.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23069..24100
                     /locus_tag="LK694_001168"
     CDS             23069..24100
                     /locus_tag="LK694_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBK5052051.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSFLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24097..24864
                     /locus_tag="LK694_001169"
     CDS             24097..24864
                     /locus_tag="LK694_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBK5052052.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24964..26532
                     /gene="liaX"
                     /locus_tag="LK694_001170"
     CDS             24964..26532
                     /gene="liaX"
                     /locus_tag="LK694_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBK5052053.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26829..27149
                     /locus_tag="LK694_001171"
     CDS             26829..27149
                     /locus_tag="LK694_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBK5052054.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27149..27499
                     /locus_tag="LK694_001172"
     CDS             27149..27499
                     /locus_tag="LK694_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBK5052055.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27666..28604
                     /locus_tag="LK694_001173"
     CDS             27666..28604
                     /locus_tag="LK694_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBK5052056.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28619..29449
                     /locus_tag="LK694_001174"
     CDS             28619..29449
                     /locus_tag="LK694_001174"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBK5052057.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29493..30518
                     /locus_tag="LK694_001175"
     CDS             29493..30518
                     /locus_tag="LK694_001175"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBK5052058.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHHASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30533..31471
                     /gene="galU"
                     /locus_tag="LK694_001176"
     CDS             30533..31471
                     /gene="galU"
                     /locus_tag="LK694_001176"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBK5052059.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            31596..32036
                     /locus_tag="LK694_001177"
     CDS             31596..32036
                     /locus_tag="LK694_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBK5052060.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32039..32641
                     /locus_tag="LK694_001178"
     CDS             32039..32641
                     /locus_tag="LK694_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBK5052061.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(32704..33807)
                     /locus_tag="LK694_001179"
     CDS             complement(32704..33807)
                     /locus_tag="LK694_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBK5052062.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            34017..35018
                     /gene="ccpA"
                     /locus_tag="LK694_001180"
     CDS             34017..35018
                     /gene="ccpA"
                     /locus_tag="LK694_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBK5052063.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            35373..35768
                     /locus_tag="LK694_001181"
     CDS             35373..35768
                     /locus_tag="LK694_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052064.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            35768..36019
                     /locus_tag="LK694_001182"
     CDS             35768..36019
                     /locus_tag="LK694_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052065.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            36062..36457
                     /locus_tag="LK694_001183"
     CDS             36062..36457
                     /locus_tag="LK694_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052066.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            36454..37710
                     /locus_tag="LK694_001184"
     CDS             36454..37710
                     /locus_tag="LK694_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HBK5052067.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGQLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            37722..38045
                     /locus_tag="LK694_001185"
     CDS             37722..38045
                     /locus_tag="LK694_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052068.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            38038..38304
                     /locus_tag="LK694_001186"
     CDS             38038..38304
                     /locus_tag="LK694_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052069.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT        12859 a         6514 c         8261 g        10700 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact
     2521 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2581 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2641 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2701 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2761 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2821 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2881 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2941 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     3001 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     3061 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3121 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3181 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3241 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3301 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3361 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3421 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3481 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3541 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3601 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3661 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3721 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3781 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3841 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3901 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3961 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     4021 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     4081 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4141 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4201 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4261 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4321 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4381 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4441 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4501 gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta
     4561 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
     4621 cccagtatac cacaatcgta agctctcgtt agttcgagag ctcttttttt tgctttgcta
     4681 ccttgttcct tgaagattct gctgatttaa gatagagtta agttaactac aagaattgga
     4741 gggatccaca tgaaatggaa agaaaaagta gacgaagcag ctgaaaagtt atatgatttg
     4801 gtcaaatcag aaaaatacaa tattgaagtg aatattccga aaaaaggcgg aaaagcggtc
     4861 caggtaaaat caaaacgccc aacgaatcat acaaaaaaat ggatggctaa aaaccgctaa
     4921 taataacaaa agatccataa gtcggcattt tcatcgattt tagggtcttt tgttattttc
     4981 taggttgaac atatctaaaa aattgtttct gacaatagga gtaggaagaa cgatgtgtgt
     5041 ctaaatcctc tgttgatgga tagaaacctt tatctttttc agttgtgaat caggtatata
     5101 ccagttataa tagaagaaac gaaaggacgg gtgtacgtta tgtcgaatca attacctgta
     5161 tatatacaga ttcatgacca attaaaaaat gaaatcgaac aaggtatatg gaaagtcgga
     5221 gaccgtcttc cttcagaaag agaattagca gtgaaattca atgtcagccg tatgacgttg
     5281 agacaagcaa ttcaaaactt ggcagatgaa ggaatcttag aaagaaagat cggttcgggt
     5341 acatatgttg cgcgagaaaa agtacaagaa aaaatgtctg gtgcaacaag ttttacagaa
     5401 attatggaat cgcaaaatcg agtaccgtct agtcggacaa tctcttattt cttgacttcg
     5461 ccaagctcaa gtgaaatgga aaaactaaac ttgagtaaag atgacacgat cttgcgaatg
     5521 gaacgtattc gtttcgcaga tgatgtccct atttgttttg aagttgccag tgtgccacaa
     5581 aagctgatcg atggctacag caaagcagag attactgaat cgttgtatcg cacgctagaa
     5641 gaaaaaggcg ggaaaaagat cggtccagcg aatcaaacga ttactgctgt gttggcttct
     5701 gagaaaattg ctgactattt agaggtaaaa cgaggagatg cagttcttcg tttgcgccag
     5761 atttcttatt ttgaagacgg tacgccgttt gagtatgtgc gtacgcaata cgtaggaaat
     5821 cgttttgagt tttatttgga aaaataaaaa acaggtgatc cctgattcct tacttagaaa
     5881 taaaagagaa tcagaatcac ctgtttttat atatgatcag ttaccagttg ctcgtgataa
     5941 gaaacaggac cctcttcaaa ttggacttgt ccgttcagca gctcaatgat ggattcttta
     6001 aagtggtcag cacttttttc atctacagca cagatgacgc taacttgatc agtatattgg
     6061 atttctttta taaaaaaatg ttggagttca agataattct gtaattttcc tagttgcgga
     6121 tacgtgatca tcacagagat ctcctgttgc aaggtacctt ctacgattcc aatgtcagac
     6181 aatgcttgag agacagcatg agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt
     6241 cctccaaaat atcgagtgac tactgcagct acattgatca gttgattttt tttgaggact
     6301 tccaacatcg gtacgccagc cgttccactt ggttctccat catcgctgct tcgctggatt
     6361 tcattttttt ctccaatgat gaaggcgctg cagttatggt tcgctttcca atgttctttt
     6421 tttatttctt gaatgaaact ttttgcttgg tcctctgatt ctattcgtct aagagaacag
     6481 ataaagcgtg attttttgat ctcaatctca ctttgtccat tttcttttat tgtacgatag
     6541 cgctcgatca tcttgtcacc tctttttttc attttcttta ttcagtataa ataaagttct
     6601 aggttgtttc aacactgtaa cggttgcgca atcgctgtag tatgcgataa taaagacaaa
     6661 ggtaggtgga tctggggatg caggaacgaa tgaaaaagta cgatgcaatt acgcattatt
     6721 tgaagaacaa tggcggctct caagtaacgt tgacctttac ccaatttgac gaactgctgt
     6781 ttcccagtaa cggacttccc aaaacagcaa gggaaagtac agattggtgg gcaaatgatt
     6841 ataaacaccc tgaaaaaggt gcctatggct ggataaatgc aggctatgaa gtagttgtca
     6901 tcaatttgga caaagaatat gtcgtgttca ataaattagt gaaatctagt tggctatttg
     6961 attaaataaa ccgcgtttag aaaacgttga caaatttctt tctttttata taatagagta
     7021 acagacgcga agaaaacgtt tgcgtagttt gttttaaaaa ggagagaaaa gtatggctaa
     7081 aaaaacaatc atgcttgtat gttcggctgg tatgagcaca agtttactag taacaaaaat
     7141 gcaaaaagct gctgaagcaa aaggaatcga ctcggatatt ttcgctgtgt ccgcttctga
     7201 cgcagataac aatcttgcaa ataaagatgt agatgtactg ttattaggac ctcaagttcg
     7261 ctttatgaaa gctgattttg aaaaacgtct ggaaccaaaa ggcattcctt tagacgtcat
     7321 caatatggct gattatggta tgatgaatgg cgaaaaagtg ttagatcaag caatcacgct
     7381 cataggtgaa taaatagaac agtataacta tcgaacaagc gatccaaaaa atagatcgct
     7441 tgttttttcg tatatttttt tagggcgaat aatatgaatc atttttgggg tagaaggctt
     7501 ctgacgagag atattaaagc aatagactgt gaggaggtaa atttaccaaa ttatgagaag
     7561 atcgcagcag tggaacgttt gggcaactgg attctttgtc atcgctgtgg aatgaggacg
     7621 tccgtcattg atggacagct tgcagattac ggttattttt gtacacattg tctatattta
     7681 gggcgatgcg acactcaaca agatttatat ttgtttgatc agcctgaagc aaaacctcga
     7741 gaagtcgtct ttttttggac aggacagctt actgaaaaac aaacggaaat cgcaaagaga
     7801 atactttgcc attcagaaaa aagacatcat cttatttggg ctgtaacagg tgcagggaaa
     7861 acagagatgc tttatcctat tttagtggaa actttaaaag ctggaggaag agtagcgatt
     7921 tgtacaccgc gagtcgatgt ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca
     7981 gaagaaaaaa tcacgttgct ccatgggaat acagcaacgg cttatacctt tacttctttt
     8041 gttatctgta cgattcatca gctataccgt ttttatcggt cctttgattt aatagtgatc
     8101 gatgagatcg atgcgtttcc ttataacggg gatgcaggat tagctcatgc tgtgcaaaca
     8161 gcgattaaac cagacggacg attcatctac ttaagtgcca cacctgataa aaagttacta
     8221 aaggaaatca agcagacgtt tgacatccac aaacttgctt tgcgctttca tcggcgttta
     8281 cttcctgaac ccgtcttggt tttcatgaat aattggcgac aaaaatgcaa gaacaagaag
     8341 aagataaaat ctttggtgcg gatcattcat caattaatta aagaaaacca tatcctttta
     8401 ttttgtccaa gtatcacgat gatgcatcgt ttgaaagcca cacttcaaga ggctcttccg
     8461 agatatgaga tcacagatgt ttcttcaaga gacaatcagc gggcggaaaa agtccagaaa
     8521 atgcgagaag caaaatatga catactgtta accacgatga tccttgaacg tggagtcaca
     8581 tttgaaagaa tatccgttat cgttttagga gcggatcatc cggtttttac taaatcttct
     8641 ttggtacaaa tcgcaggaag agccgatcgt aaagggcctt atacgaacag tcaagtatac
     8701 tttttctatg aagaaaaaac tgcagctatt cgaaaagctt gtcaggaaat caaagaaatg
     8761 aatgaagaag ggaagaagat gctgtgaggt gcagttgctg cagtaaagat atcgtccaaa
     8821 atttaacatt cagcgagatt attggtgttt cgaaatacag attacctcag acttgttcag
     8881 tttgtcgcgc acaatttcat ttattggaaa caagaggctg ttctggctgt caaaaaccaa
     8941 tccatttcaa tctagagagt acgaaaaaga aagtaaagtc tattcccgtt tactgcagtg
     9001 actgtcaaga atggaagaag aaatatccga cctatgattt tcagcaccgt gccttttttg
     9061 tgtacgatga aaatatgaag gcctggctca atcaatacaa gtttatggga gatatccgac
     9121 tagcaggcac ctttgcatat aaatggacgc agataaaaaa agaatacccg acatacatct
     9181 attgtccaat acctttatct gaaaaaaggc gaacagaaag aggattcaat caagtaagcg
     9241 agatgttgaa agcagcaaga attccttttc agatgctact caaaagagag aagcatctgc
     9301 tggctcaagc aaaaaacacg cgaaaggaaa gattggagat gcctcaacct tttgaacttg
     9361 cagtcacctt ttctaagata caaaataaaa aaatattgat tattgatgat gtctatacaa
     9421 caggacagac tcttttccac gcagcagatt gtttgcaggt cgcacagccg aaaatcatcc
     9481 gaaccatttc tttagcaaga tgaattagat gtggaaattt gatagcgatt taatttgcaa
     9541 ataacctaga gttcgatata atagaattaa gaagagggaa agagtggtcc ttctccttct
     9601 tgattgtggt agacgaaagg ggtaattttt atgtttagat ataatgtacg cggcgaaaat
     9661 atcgaagtaa ctgaagcgat tcgcgactac gttgagaaaa aagtaggcaa actagaacgc
     9721 tatttcagtg acgcacccga tgcaacggcg catgtgaact taaaagttta tacagaaaaa
     9781 acagcaaaag tggaagtaac gatcccatta ccttatcttg tattacgtgc cgaagaaact
     9841 tctccagatt tatacgcaag tatcgatttg gttgttgaca aattggaacg ccaaatccgc
     9901 aaattcaaaa caaaaattaa tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct
     9961 actgctttat tcaatgaaga ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt
    10021 acgaaacgcc tttcattgaa accaatggat agtgaagaag cagtcttaca aatgaacatg
    10081 ctgggacata acttctttat ttttgaagat gcagaaacaa atggtacaag tatcgtttac
    10141 cgccgtaaag atggaaaata cggactgatt gaaacagact gatcagcctt acttaaataa
    10201 caacgaaaac ttgcagcagc tactgacagc tgccgcaagt ttttttgagt ttcctcctct
    10261 tttagatgaa atatgaataa attatgaagt tcttacttta atacgcttaa gatatttcaa
    10321 ttatctataa ctaatgataa aatgaaaagt aaagtctaca tagtctggat aatacacaga
    10381 aggaaaagga gattacacaa cgcatggcca atttgattcg ttcacttatc gaaaacgata
    10441 agaaagaact aaaacgttta gatagcattg caaaaaaagt tgaagcatac gctgaccaaa
    10501 tggcagcatt aactgatgaa caattacagg caaaaactgc tgaattcaag ggcagatacc
    10561 aaaaaggaga aacattagat caattacttc cggaagcttt tgcagtcgtc cgagaagcag
    10621 caaaacgtgt attggggtta tatccacacc acgtacaatt gatgggtgga atcgtgcttc
    10681 acgatggaaa tatccctgaa atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc
    10741 cagtatatct aaatgctctg actggcgaag gggtacacgt agttaccgtc aatgaatatt
    10801 tagcaacccg tgactcgacg gaaatgggcg aactttataa tttcttagga ttaacagtgg
    10861 ggttgaatat caactcaaaa tcttcggatg aaaaacgcga ggcttataat tgtgatatca
    10921 cttatagtac gaataatgaa ttaggatttg actacttgag agacaatatg gtcgtttacc
    10981 gccatcaaat ggtacaacgt ccgctgaatt atgccattgt cgatgaagtt gactcaatct
    11041 tgattgatga agcgagaacg ccgctgatca tttcagggca agcagaaaaa tccacagctt
    11101 tatatacaag agcagacaac ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg
    11161 atgtacaatc aaaaacaatc agtttaaccg aagcagggat cgaaaaagca gaagaaacat
    11221 tcggtttgga taacttatac gacattgaaa atactgcatt gacccatcac ttggatcaag
    11281 cattgagagc caactatatc atgcttttag atatcgatta tgttgtccaa gacggaaaag
    11341 tactgattgt tgaccagttc actggtcgga tcatggatgg acgccgttat tcagatggac
    11401 ttcaccaagc aatcgaagct aaagaaggcg tagagatcga agacgaaacg aaaaccatgg
    11461 cgacgatcac tttccagaac tatttccgta tgtacaagaa acttgcggga atgactggta
    11521 cagctaaaac agaagaagaa gaattcagag aaatttataa catccaagta taccagattc
    11581 cgacaaatcg tccggttatc cgtgatgatc gtccagattt gctttaccca acattacaaa
    11641 gcaagttcca cgctgtcgtg caagatatca aagatcgcta ccgcaaaggt caacctgtgt
    11701 tagtcggaac tgttgcggtt gaaacatctg agttattatc agatatgctg aatcaagaac
    11761 gtattcctca tgaagtgctg aatgcgaaaa atcactttaa agaagcagag atcatcatga
    11821 atgctggtca aaaaggtgct gtgacgattg ctaccaacat ggctggtcgg ggaacagata
    11881 tcaagttagg acttggggta agagaacttg gtggtctagc agttatcggg acagaacgac
    11941 atgaatctcg tcgtatcgat aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg
    12001 gtatgtcaca gttctacttg tcattggaag atgatttgat gaaacgattc ggttcagaac
    12061 gaatcaaatc tttcttagat cggatgaaga tcggtgagga agatgctgtt atccaaagca
    12121 agatgctgac gaaacaagta gaatctgcac aaaaacgtgt agaaggaaat aactatgaca
    12181 cacgtaaaaa cgtcttacaa tacgatgatg tgatgcgtga acaacgtgaa gtaatctata
    12241 aacagcgaca agaagtaatc atggaagaaa aaagcttatc caagccacta atgaacatgg
    12301 tcaaacggac aatcagtcgt gtagtagatg cgcatacaca attagagaaa gaaaattgga
    12361 atttggaaag tatcatcgat tttgccggca gtactcttgt ccatgaagat agtattactc
    12421 ttgcagatat cgaaggaaaa acagctcagg agatcaaaga gtatctaaac gaacgagcac
    12481 aagaagtatt taataccaaa gcggctcaac taaatggtcc agagcaactg ttggaatttg
    12541 aaaaagtcgt gatcttgcgt gttgtggact ctaaatggac agaccatatt gatgcaatgg
    12601 atcaattacg tcagtcaatc ggcttacgtg cttatggaca gaacaatcca ttagtcgaat
    12661 atcaaacaga aggctataag atgtttgaag atatggtagg cgccatcgag tatgaagtca
    12721 ctcgaatctt tatgaaagct gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag
    12781 gaacagcttc tcactcagaa gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa
    12841 aacagccaat ccatgtgaac aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga
    12901 aaaaatataa gaactgtcat ggaaaaaatg caaactaatt tctgacagca ggattggtga
    12961 gacaaattct ttttttaaga attcgtctca ccttctttct gttatttgct gattctgtta
    13021 gactagatat aatgaaaaaa gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat
    13081 tcgaaatctc ctgacagaaa tgcaggaaaa ggtcacaagc ttcaggaggt ctctttgact
    13141 tagaccagct ggaagaagac attgcggaag cagaagcaaa aatggctgaa cctggttttt
    13201 gggatgatag cgaagctgct caaaaagtga tcagtgaaaa caatgcaaat aaagaaactt
    13261 atgatcaatt ccattctctt gctgaagaac tggaagaaat cgaattgatg gtggagatgt
    13321 tgcaggaaga acctgatgca gccatgcaag aagaagcaga aacacgtatc aatgcgctag
    13381 atgaaaaaat gaagacatat gaattatcta tgttgttaga tggtccttat gatcgaaaca
    13441 acgccatcgt tgaattgcat ccaggggctg gcggaacgga gtcccaagat tggggcagta
    13501 tgcttttacg tatgtataca cgctgggcag agcagcatgg gtatcaagta gagacactag
    13561 attatcaagc aggtgatgaa gcaggaatca agagtgtgac cctactgatc aaaggacaca
    13621 acgcatttgg ttatctgaaa tcagaaaaag gtgttcatcg ccttgtacgt atctcgcctt
    13681 ttgactcagc gaaacgacgt catacttctt tttgttcggt agatgtgatg ccagagcttg
    13741 atgaaacgat cgatatagaa atcaatccag atgatttgaa aatcgatacg taccgagcaa
    13801 gtggtgcagg tggtcagcac atcaataaaa cagaatctgc tgtacggatc actcacttgc
    13861 ctacaggaac agtagtagct agtcaggcac aacgttcaca attgaaaaac cgtgagcaag
    13921 cgatggggat gttaaaagct aagctttatc aattagaagt ggaaaagaaa gaacaagaag
    13981 cagctgcact tcgaggagaa caaaaagaaa tcggctgggg ttcacagatc cgttcttatg
    14041 ttttccatcc atattctatg gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc
    14101 aagcagttat ggacggtgac cttgatttat ttattgacgg ctatttaaaa atgcatctac
    14161 aataagaacc cattgttaaa ttgaaataaa attgtaagat tttgcaagca tgttgaaaca
    14221 ttgccatgct ataatagcta aagactgaaa aagatatgga gagaacgcca tgattgaaat
    14281 gaaggatgta atgaagaagt actcaaatgg tacgaccgct atccgcaatc tctcaattgt
    14341 catcgaacaa ggagagtttg tctatgtggt tggtccgtct ggagccggta aatctacatt
    14401 catcaaattg atgtatcgag aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca
    14461 tgatctaata gcaatcaaaa accgcgaagt tccttatcta cgcagagaaa tcggcgtggt
    14521 gttccaagac tataaattat taccaagaaa aacagtatat gaaaatgttg cgtacgctat
    14581 gcaagttatc ggcagaaaac ctcgggatat caaaaaacga gtaatggaag tgctggacct
    14641 tgttggtttg aaacacaaag ttcgagtctt tcctagcgaa ttatccggtg gggaacagca
    14701 acgtgtctct attgctcggg caatagtgaa tacgcctaaa gtacttatcg cggatgaacc
    14761 gacaggaaat ctggatccag aaaactcatg ggaaatcatg aaattattag atcggatcaa
    14821 tgcccaagga actactgtcg tgatggctac acataacagt acgatcgtaa atacgattcg
    14881 gcaccgagta attgctatcg aaaacggccg cattattcga gatcaagcgg aaggggaata
    14941 tggatacgat gattagaacc tttttccgcc atttattaga aagtatcaag agtttgaaac
    15001 gtaacggctg gatgacagtt gcttcagtca gtgcggtaac gatcacattg actttagtcg
    15061 gtgtattcat ggcagttatc atgaacgcaa ctaaattagc tcaagatata gaaggaaacg
    15121 ttgatgtctc tgtattcgta gatatcggta caaaagacga agagatgaaa aagctagaaa
    15181 aagaattgaa tgacttagat catgttaaaa aagttgaatt ttcaagcaaa gaacaggaat
    15241 tgaaaaaaat ccaaaacgaa atgggtgatt cctggaaatt gtttgaaggt gacaataacc
    15301 cgttatatga tgtgtatgta gtaagtgcta ctgatccttc ttatacaaaa caagtagcag
    15361 aagaagcagc agatttgaag aatgtttctc gttctgatta cggtggagct tcctcagatc
    15421 ggattttcca aatcgctggt gcagttcgga catggggatt agttgcagcg gcattgctgc
    15481 tgttcgtagc gatgtttctc atttcgaaca cgattcggat cacgattttg tctagacagc
    15541 gtgaaattca aatcatgcgt ttagtcggtg caaaaaatgg atatattcgc tggccgtttt
    15601 tccttgaagg tggctggatc ggtctgatcg gtgcgattct tccgattttg atcatcacat
    15661 ttggttatgc atggtttttc caattgatca atccatcatt attacgttcc cattattcat
    15721 tgattcatcc acaaaatatc gtttggaaaa tcaatctttt aatggtcggc attggtgtga
    15781 tcattggttc acttggctcg atcatttcta tgagacgttt cttgaaattc taataccaca
    15841 atataaataa aatgagaaag ttggacagtt tcgttccagc tttctttttt tattggctag
    15901 gagaatagaa ggaagcggct tttttccctt tactttaaaa aacaacttgg gtattcttaa
    15961 gttggtagaa gaagaggtga agccgtgaaa attatcgttg acaatttttt tacgatcctt
    16021 ttgattatga atattttgct ttcctttatc atcgtttttc gagaaagaaa ggaaactgct
    16081 caaacatggg catgggtgct cgttttgatg ttcattccag ttgtaggatt tattttatat
    16141 atttttttag gaagagggat ttccaaagag aaaatctttg atttgaagat ccaagggaaa
    16201 atcggtaaga acctggaaat tgaagaagac aaacaggcat tgatgcgagg gctttatcct
    16261 catccgccta ctggacaagt agatgtgaaa caattgatct atatgctcac ggtatttgaa
    16321 agtacgctct atacgactaa aaatgaaatc actttattta cagatggtcg tgaaaaattc
    16381 gatgcgctga ttgaagatat caagcaggca gaagaccata tccacttcca gtattatatt
    16441 tatcgcagtg atgcattagg tgaagaagtt cgagatgcat tgacagaggc ggcaagacga
    16501 gggggaaaag tcagagtcct tcttgatgca tggggttcca cacaagtttc ttccagcttt
    16561 tttcagaatt tgaaaaaagc aggcggagaa gtcgcatttt tctttcctct atttgttccg
    16621 tatattaatc ctcggatcaa ttatcgcaat catcgtaaaa tcgtagttat tgatgggaaa
    16681 atcggatata caggtgggtt caatgttgga aatgaatatc taggattggt aaagaaattt
    16741 ggttattggc gagacaatca tttgcgtatt tacggagaag cggtgtatac cttgcaaaac
    16801 cgttttttga tggactggaa ttctcagcat acaaaagaag taggctattc tcccaaattt
    16861 tttccgtcca ttcactctac tggtgatata gctgttcaaa tcgtgacaag cggaccagat
    16921 accgaacatg aacaaatcaa aatgacttat ttgaaaatga tttcgttagc caaacgggag
    16981 atattgatcc aaacgcctta ttacataccg gatggatcga ttcatgaagc actgaagttg
    17041 gctcttcttt caggcgtaaa agttcatatc caaataccta acaaaccaga tcatcttctc
    17101 gtttactggg caacgtattc tttcgctgca gagctaatcg agtatggagc aaggatcgaa
    17161 acatatgaga atggatttat tcatgcaaag acgatgatta tcgatggtgg cattgtttca
    17221 gttggctcag caaatatcga tgttcgttca tttcgcttgg actttgaggt caatacactg
    17281 gtttatgatg aacgaatggc ttcacgtgta agaaaagctt tttttgaaga ctcgaaaatt
    17341 tctacgcatc tcacaaaaga aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa
    17401 ggattagcaa gattgatttc gccgctcctt taaaacataa aaaggacatg agaaccatca
    17461 acagatggag atctcgtgtc cttttctaac ggggccattt ctgagagtac gtcagaaatg
    17521 gagaaagcgt ctcgcatgag cgaaattaac tttggggtat ctcttgtttt ttggggaaag
    17581 caagaaataa gctaatatat gctattaaaa gagacacatt tcaaagccag tatagcatag
    17641 aaacttttaa tgagatagtt ttttttacaa ttatgagaat ttattttgtt atttaatgat
    17701 tttgattcgt tttcatcaag taatgaaaat gataaaataa acatacttta ggattaaagg
    17761 agatttctaa tgaaaaaact taaattattc ttaccaataa taggattagc tattctttta
    17821 tcaggctgtt caaagtggat tgatcgagga gaatccatta cggctgttgg ctcttctgct
    17881 ttacagcctt tagtagaaac agttgctgaa acttaccaga cagaaaatcc tggaaaattc
    17941 atcaatgtac aagggggcgg aagcggaact ggtttgtctc aagtacagtc aggagcagtc
    18001 gacatcggaa actctgattt gtttgcggaa gaaaaatcgg ggatcgatgc ctcagcatta
    18061 gtcgatcatc gagtagcagt tgttgggatc actcctattg tgaacaagga tattggcgta
    18121 aaagacatca gtatggaaaa cctgaaaaaa ctatttacag gcgagatcaa aaactggagt
    18181 gaagtcggtg gaaaagatca gccagtagtt atattgaacc gcgcttcagg aagcggaacc
    18241 cgtagcacgt ttgaaaaatg ggtattggac ggagaaacag ctgttcaggc acaagaacaa
    18301 gattcaagcg gaatggtgcg ccagatcgtt gccgacacac ccggtgcaat cagctatgtt
    18361 gctttttctt atgtttccga tgatgtagat acgttgaatg ttaacggtgt agaaccgaca
    18421 gatgaaaatg tgacaacgaa caaatggacg atttgggcat atgagcatat gtacacaaga
    18481 gatcatccaa aaaaactgac aaaagaattt ttagactata tcttatctga tgaagtacaa
    18541 gataacatcg ttggagaact aggttatatc cctgtttctc atatgaaagt cgaacgtgat
    18601 tggcaaggaa atatcgttaa agagtaatct ttacacaaaa tttacaaact tagctaaact
    18661 tttacatgtt attaaaacca aattgatgtt caatttgtac actgagtgag tatgttaaac
    18721 ctgattaagg gggagtcatc tgtggaagat gtgaaaaaag tattattaac aaaatcaaaa
    18781 cgttcaaaaa tggaacagcg tggaaaattc atcagttttt tatgtatcgc gctgatcgtg
    18841 ttggttgtat tatctatttt ttattttgta gctagtaaag gattggccac cttttttact
    18901 gataagatca atgtatttga ttttctgttt ggtacagatt ggaatccaag ccaaatcgga
    18961 gcaaatggga aaccaatggt aggggcattg cctatgatca caggttcgtt tattgtgacc
    19021 tttttatctg ctatcgttgc cacgccattt gccattggtg cagcagtatt catgacggag
    19081 atctctccta aaaacggaac gaagatctta cagccggtca tcgagctgct tgtaggtatt
    19141 ccttctgtcg tttatggttt tatcggttta tcagttatcg tgccttttgt cagaagtatt
    19201 tttggcggga ctgggtttgg tatcttagct ggtacatttg tgttgttcgt tatgatcttg
    19261 ccgactgtca caacgatgac tgttgatgca ttaaaggcag tgccacggca ctatcgggaa
    19321 gcttcattag cgcttggtgc cacaagatgg caaacaatct acaaagtcgt gctacgagca
    19381 gccgtgcccg ggattttaac agcagtcgtt tttggtatgg ctcgtgcgtt tggtgaagcc
    19441 ttagctatcc aaatggtcat cggtaatgca gcattgatgc caacaggtct tactactcct
    19501 gcttctacat tgacgtccat cttaacaatg ggaatcggaa atacaatcat gggaacagtt
    19561 gaaaataatg tgttatggtc attagcgctg atcttgctat tgatgtcatt attctttaat
    19621 atcgtgatcc gtatgattgg gaagaaagga gagctgaaat aatgaacgca aaacgctcag
    19681 ataaaattgc tacaggtgtt ttatatatag tttcggggat catcgtattg attttagcag
    19741 ctttactact ttatatctta gttcggggaa taccgcacat ttcatggtcc ttcttgaccg
    19801 aaccttccaa agcctaccaa gaaggcggtg gaatcgggat ccagctattt aactcgattt
    19861 atttgctttt gatcacgatg atcatcagtt ttcctatttc tttaggtgcg ggtatctatc
    19921 tttcagaata tgctggaaaa aactggctaa cagatgtgat tcgcacgtcg attgaaatct
    19981 tgagctcgtt gccttctgtc gttgtcggtt tattcggttt cttggttttc gttattcaat
    20041 tccagtatgg tttctcgatt atttctggtg cattggcttt gactgtattc aacttgcctc
    20101 ttttgactag aacagttgag gagtctttac aagcggtcca ttacacacaa cgagaagcag
    20161 gattggcctt aggtttgtca cgctgggaaa cagtgataaa agtggtggtt ccagaagcga
    20221 caccagggat tttgactggt gtgatcctaa gttctggaag aatttttggt gaagcagctg
    20281 cattgatcta tacggctgga caaagtgcac ctgcgttaga ttttacgaac tggaatccat
    20341 taagtgtttc aagcccgatc agcattttcc gtcaagctga aacattagct gtccatattt
    20401 ggaagatcaa tacagaaggc acgatgcctg atggtgtttc tgtttcggct ggcgcttctg
    20461 ccgtgttgat tattgctgtt ttactgttca acttcggcgc tcgtgcgatt ggcaaacgat
    20521 tataccgaaa aatgacttca gcctaaggag aagaagcaaa atgaaaaaat ataactggaa
    20581 tgaaacaaat gtcatcacca tgccaaaaga gaaagaagta gcactatata cggatgatct
    20641 acatgtctgg tatggcgaca atgaagcaat caaaggagtg gatctggaat ttgaaaaaaa
    20701 taagatcact gcactgattg gtccttccgg ctgcggaaaa tctacgtatc ttcgttcgtt
    20761 gaatcgaatg aatgatggga tcgctaatac gaaaatcact gggaaaatca tgtatcaagg
    20821 cgtagatatt aatgcgcctc aagtggatgt ttatgagatg cgcaaacgga ttggcatggt
    20881 ttttcaacgt cctaatccgt tcagcaagtc gatttatgac aatattacat ttgctttgaa
    20941 acagcacggc gaaaaaaaca aaaagaaatt agatgaaatc gttgaaacaa gcttgaaaca
    21001 agctgccttg tgggaccaag taaaagacag cttggacaaa agtgccttgg ctttatctgg
    21061 cgggcaacag caacgtttat gtattgctcg tgcgatcgcg atgaaacctg atatcttgct
    21121 gctagacgag ccggcaagtg ctttggaccc tatttcgaca ggaacggttg aagaaacact
    21181 tgtaaacttg aaagaagact atacgatcat catcgtgacg cataacatgc aacaagcagc
    21241 tcgtatcagc gactatactg cctttttcta tttaggaaaa gtaatggaat atgacaaaac
    21301 taacaagata tttaccagac caaaaattca ggcaaccgaa gattatgtgt ccggtcattt
    21361 tggttaagac ggaggcctca acatgacaaa agagattatt tcatctaaag atcttcatct
    21421 gtattatgga aagaaagaag cactaaaagg gatcgatcta agcttcaatc aaggggaaat
    21481 cactgcgatg atcggtcctt ctggttgcgg aaaatccact tatctgcgtt ctttgaaccg
    21541 gatgaatgac ctgattccga atgtaacgat caccggaagt gttttatata aagacaaaga
    21601 catctatggt ccaaaaacag atgttgttga actaagaaaa gaaatcggca tggtattcca
    21661 gcagccaaac ccatttcctt tttctatcta tgaaaatgtc atctatggat tgaaattaaa
    21721 aggggaaaaa gataaaaaag tccttgatca agtagttgaa gaaagcctga aggcagcgtc
    21781 tgtttgggat gatgtgaaag acaaattgca taaaagtgct ttatctttat ccggtgggca
    21841 gcagcagcgt gtatgtatcg cacgtgtttt ggcagttaat ccggaaatca tcttattgga
    21901 tgaaccaaca agtgcgcttg atcctgtttc tactgggaaa atcgaaagta tgctgttaga
    21961 attaagagag cggtatacga tgatcatcgt gacccataat atgtctcaag cttcacgtat
    22021 ttcagataag acggcattct ttttagatgg tcacttgatc gaatacaatg atacgaagaa
    22081 gatattcatg aatccagaaa aacaagagac agaagattat atttcaggtc gatttggata
    22141 aggaggaaac ggaatgctac gtacacaatt tgaagaagaa ttgctgaatc tccataatca
    22201 gttctacgaa atggggatga tggtgagcag cgccgtgcac aagtcagttc gtgcatacat
    22261 caaacacgat aaaatcttag cccaagaagt tatcgaaaac gatataaata tcaataacat
    22321 ggaaacacgc ttggagaaga aaagttttga aatgatcgca ttgcaacagc cagttacaac
    22381 tgatttgcgt atgatcatta cggtcatgaa agctagctcg gatttagaac gtatggccga
    22441 tcatgcggtg tctgttgcta aatccacgat tcgcgtaaaa gggcagacga gaataccgga
    22501 aatcgaaaaa gagatctccg atatgtccga ttatgtgaaa aaaatggtgg acaacgtcct
    22561 agttgcttat gtcaaaacag acgaaaaaga cgcacgtacg atcgccaata tggatcaaag
    22621 agtcaatgac tatttcaatc gaatctataa tgcaacaatc aaaaatatgc aggaaaatcc
    22681 tgagacagtc atcagtggaa cagactactt gaatgttgcc agttatctag aacggatcgg
    22741 agactatgtg acaaacatct gtgaatggat cgtctacttg gctacaggga aaatcactga
    22801 gttaaatacg aaccacaatg agattttcta aactgaagct gtgattatgc cgtttgttta
    22861 ggaaaagagg ttgtgaaatc agcggttgtg aaaaagctgt tctgcatcaa gtaataagaa
    22921 cagtcaaaca aaaaatagct cctcatattt ttcgtttggc tggacttatt accgcagatg
    22981 caagcttttg aacaccgttt attcgggaaa gggtttgtga caaaacatgt cacaaaccct
    23041 tttcatcatt tcatagaaag gataaagaat gcaaaaaaaa attagcatca tcactatatt
    23101 ttttatttgt gtgcttactt catgggttat tcagcatcaa gccgctttat atgaagtacc
    23161 tgtcatcgag atagaaaaag tacgaataaa agaggatcat caattgatta caggaaaact
    23221 gatgaacacg gatcaaaaag gagaatcgat ttatttatcg gttccttatc aaaaaaatca
    23281 aatagaaaca gcaaatataa aagcgagaca aatgtatcta gtccaaatgc atggagatga
    23341 atggcagcta gtagcaaaaa aaaatgatgg ctggcttttc ttcttcacaa gtatcttcat
    23401 tggcgccatg ttgcttattg gtggaaaaca gggtctattt gctttaggaa gtctcatcat
    23461 caatatcgtt ttacttttac ttttgttgct catctttact aaaacagaag ggatttctct
    23521 attaaatata tctatcctgt tcgtcgttat agggatgatt attagcatat tggctttaga
    23581 tggatggaat agaagcagta taatcaaaat ttcagctgca gttacgagtg tttttttagc
    23641 attttttatt tgtcttttga caatgaatca ttggaaagac caaggactgc gctatgagga
    23701 actgaattat ctgacacgac cgtatcgttc tgtttttctt tctagcgtac tgattggaac
    23761 agctggagga acactcgata ccgttatcac agtcattgcg acattagaag aattgactaa
    23821 gcgacaacca gaaataacag cgaaacagtt atggctttca ggaaggcaag tgggtcgaga
    23881 tgtgattggc tcgatgacca atatcctttt gttcgtctat atcagtgggt cagttccatc
    23941 gtttctttta tatttaggaa atcaatggtc gttcaaagaa acgatagaac agcatttatc
    24001 attggaattt ttacgcgtca ttgctggcag cttaggaata gtattatcga tccctatctc
    24061 ggtcctcttt tttctgactg taaggaggct gaaacgatga atgtacttat tatacttgtt
    24121 gggattttag caatcagcgt gctacttatt ggcggtacgc aaggtatgta tattctttta
    24181 ggcttattca tcaatttagg gatctttttc ctgttgttat tcggttatca tcagaagtgg
    24241 ccgatactgg cattgtcggt cataggattt ttattgattg ctgtggttat cttatttttt
    24301 atcaacggtt ataatctaaa aatgcgtgca gcttttgtga gtatactgat ctttttattt
    24361 tgtttcctac tattgcttcc tatcactgat tttttagcaa tccaaggttt tacatcggta
    24421 gaactagaag aattatctgg cttagataag actttagcga ttgattttag attacttacg
    24481 agatcgttgc ttctgattag tcttagtggt gctgttttag atgcgagtgt agcaatttct
    24541 agtgggacat tcgaagtata tcaggcaaat ccgcaattat ctttttacca attgcgccat
    24601 tctagtttcg ctattgctaa aaaaatattg gcgtcaacag taatgacact tctttttgca
    24661 ttcatgggaa gttctttagc gttgatcctc tggtttattg atttagacat tccttttcag
    24721 caagtgatca atgaaaaatc atttgttcta gaatatacga tggctattct taccacattg
    24781 tccgcactac ttgttcttcc tttaacctgc gtgactacag catatctctt tacaaaaaag
    24841 aaagaacaac tcaagatgga gtaaaagtta caacttaagt cctatgacat ttttactgaa
    24901 tcagtgcata atatggatgt aaggaaaagc ttgcaaacaa ttttgcttaa ggaggcaatt
    24961 tccatgaaag aaagagaacg tattttagat ttagtgaaaa aaggaatcct ttcgactgaa
    25021 gaaggcttag atttattaga aagcatggca actgaaaaag acgaaaagca aatcaaaaaa
    25081 gaagcagaca aggtaactgc tagtcataaa gaaaaagatc aggcaagtca attgatcgat
    25141 aaacttgaaa acggcgaaga agaaatttct gagccagttg atccaaagga aagagaaaga
    25201 caagatcaag aaaacttaga aaagatctta gatgaactag caactgaagc gaataaaact
    25261 tcggcgcgtc tagatgaagt aaatgctcaa ttcgctgaca ataaggcagc acgcaatgag
    25321 aaacaggaag cattgatgca gctgaataca aaagaagaat taggcgagtt gacagaagaa
    25381 gaattagcac agcgtcagac gttagaagca gaaatcaaag aattagaagc tagtggcgat
    25441 acactgttag aagaacaatt gaaattagaa gcagaattaa aagatatccg taagaatcaa
    25501 tggtctgaga aaaaagaaac gtttactgat aagtttgaat tgccagatga ttggaaagat
    25561 caagcaacag atacactgaa tcaagtaggc gaaaaaatgt cagaagctgg atcacagtta
    25621 ggcaaattct tgaagaaaac attccaaact gtttcagaaa cagtgaatga caacatggaa
    25681 tggaaagatg tcagcttacg tgtccctggg attgcgacaa cgaaatttga gcatgaattt
    25741 tactatgaag ctcctgcagc aagtatcctt gatatcaagg cagcaaacgg aaacgtgacg
    25801 ttaaaaacgt gggacagcaa cgatgtgaaa gttgaagcta agatcaaact ttacggaaaa
    25861 atgggtgctg aaccatttga agcattctct gaacggagcc agattgaggt taatgaagat
    25921 catatctcct tccaaattcc aaataaacgt gtgcgtgcag accttgtttt ctacttacct
    25981 aaacgagtat acgaccacgc agctattaaa cttctaaatg gcaacatcat gattgaaaca
    26041 ttggaagcta aagatattta tacaaaatct acgaatggta acatcatcgt ggaccaatta
    26101 actgcaacga tgctggaagt agaaggtgta aacggcaata tcgatattcg aaacgggaac
    26161 atcttagatt ctattatcga aacagtaaat ggtacagtga catttggtgc gacagcagaa
    26221 aacctttctg tgtctttggt aaatggtgat gtacgtctta cgattaaaga agataatttg
    26281 aaaaaagtag aagcaagttc agtgaacgga aatgtcaaag tcgctttacc agatggcatc
    26341 ggtttggaag gacatgctaa aacaagtctt ggaagtatca acagccgtct gtccaattac
    26401 gaagtagttc gtgagaaaaa agaacggaca aaccaaatgt tgcaattccg tcgtgtttca
    26461 gatggtgaaa tcgcgcaagt gcaactttca acgacaacag gaagtatcta tttaaaagat
    26521 acagataaat aaagaggttg tgaaagagat gttcagcctt gagcaataag aagcgatttt
    26581 gtaaaatagc ttcccctatt ttacaaaatt gtgccttatt gccgatagtc tatctcttga
    26641 acaccgttta ccaagagatt atgaacctgc caattatcgc cgagtcacaa ggaataattt
    26701 cagaaaaagc tcgtcacgtt ttatgaatat ggtaatttgt gaccgagaat tttgcagaaa
    26761 gacaccgatt aagaagaagg ttgtcacaac gattttcttc atttaaaata gtagaacaga
    26821 ggtgagacat gatgaaaaaa aagttgacaa aatctaataa aaatgttgtt ttgacaggga
    26881 ctttagcagg aataggagaa tacatcggga ttgatccgac tgttatccgt gtgctttatg
    26941 tattcctgag cttagttgct tttggaagtc caattatttt atacatttta ttagctttga
    27001 ttatcccatc tgcgcgttct gcacgaacaa actatggaca taacaatgca tattatgaaa
    27061 aaaacaatta tcgtgaacaa actcgtgaac aaacaaaaaa acgcaaagaa gcggaaaaaa
    27121 tcgatgatga cgaatggagt gacttttaat gtcgtacttt caacgtattt tagttaatac
    27181 gttaacattc atatcattgg ctgttctttt tcctgcgatg gtccatgtga gcagtatttg
    27241 gacggccgtt cttgccagct tcgtattgtc tgtgttgaat atgtttgtca aaccgatttt
    27301 gacgatactg tccctgccct ttacattagt cacattggga ttgtttacgt ttgttatcaa
    27361 tgcagcgatt cttaaaatga cctccttttt tgtgggagca gaacatttta gtttttcttc
    27421 attcggtgca gcgttgttag tagctatctt gatgtcgctt gtcaatgtaa ttgtcagcga
    27481 acataacagt agaaaataaa agaaagctgt gccgaactat cggcgcagtt tttttattta
    27541 gaatcaggaa tgcgttttct attacattgg taattaaata aaaacgtttt ttttaattgg
    27601 tagtactttt ggctagacaa tgataaaatg atagagaaca ctctaataga aatgaggtag
    27661 catgaatgtc agaagttgta aaagttagcc aattagtgaa aaaattatct ttggaaatcg
    27721 tgacaggcga cgagaaaagc ttagaccgtg tcatcgtaac tggggacatc tcacgcccag
    27781 gactagagtt gactggttat tttaattatt attcccataa tcgtttacag ctatttggca
    27841 gtaaagaaat cagttttgcg gaacggatga tcccagaaga gcgtttgatc atcatgagaa
    27901 gattatgtag tgaagatatg cccgcattta ttatttcaag aggattacca gctccagaag
    27961 aaatgatcca ggcagcaaat gaaaatggga tagctgtact gcgttctccc atctcaactt
    28021 ctcgtttatt aggagaatta tccagttatt tagatggtcg ccttgcccct cgaacaagtg
    28081 tccacggtgt tttagtggat gtttacggat taggtgtact gattcaaggt gacagtggga
    28141 taggaaaaag tgaaacagcg ttggagttga tcaaacgcgg ccatcgattg atcgcagacg
    28201 atcgagtaga tgtttatcag caagatgaat taacggtgat cggagaaccg cctaaaatcc
    28261 tggaacatct gattgaaatc cgaggaatcg ggatcattga tgtaatgaat ctttttggtg
    28321 cgagtgcggt acgtggctct atgcaagtcc agttggctgt ttatctggaa gcatgggcaa
    28381 aagataagaa atacgaccgt ctgggctctg aagacacgac cgttgaaatc gcggaagtgg
    28441 gcattccaca aataaaaatc cctgtcaaaa caggacgaaa cgttgcaatc atcattgaag
    28501 tagccgcgat gaatttccgt gcacgtacaa tgggctttga cgcaacgaaa acatttgaag
    28561 aaagattaag tcgcttgatt gaagaaaact caggcaacga ctagcaagga gtgacaatat
    28621 gttagcacaa atcaatcgag ttgcttttga gcttgtaggg attcctatct actggtatgc
    28681 attgatcatc gtatcaggta tcattatcgc tatgtggctt agcagcagag aggctgttcg
    28741 ggtaggatta aaagcagatg atgtgacaga ttttatgttg tggggattgc ctatctccat
    28801 tattggcgct cgcttatatt atgttttatt tgatcttccc cagtatatag ctgatccaat
    28861 ccaaatattc aatatccgtt caggaggctt agctatttat ggcggactga ttgcaggcgc
    28921 gatcacgctg tattttttta ctaaatatca ttttatttct ttgtggactt ttttagatat
    28981 cgctgcacca agtgtattat tagcacaggc aatcggtcgt tggggaaatt tcatgaacca
    29041 cgaagcatat ggaccagaca cgacgcgaag cttcttagaa agtctgcatt taccgcaatt
    29101 catcattgat aatatgtata tcgaagggat ctatcgtcaa ccaacatttc tttatgaatc
    29161 actttggagt ttgattggat ttattttgct tttgttttta cgaaagaaac cacacctttt
    29221 gaaaaaaggc gaagtccttt tatgttatct aatgtggtat agtttcgggc gtttctttat
    29281 tgaaggatta cgtacagaca gtctttactt tttaggtgtg atccgtattt ctcaattatt
    29341 atctgctgtg ttatttgtcg gtacattgat tctatttatt tggcgcagaa aaaaaggaaa
    29401 tttaccggat tatgaccgct cattaggaaa aaatcaacaa attatctgat ttttagagcg
    29461 ttaaaaatga aaaaaatttc aggagtggta aaatgaatta tagagtagcg gttttaggac
    29521 caggctcttg gggtactgct ttggcacaag tcctcgccga aaacggacat gacgttcgga
    29581 tttggggaca tcatgcttca caagtagaag aaatcaacac ccaacacacg aatcaacgtt
    29641 atttaccaga tcattatcta cccgattcca tccaagcatt caccaatatg gaagatgcag
    29701 taaaagacgc agatgcggtg ctattcgtca ttccaacgaa agcaatacgt tctgttgcaa
    29761 aagaactagt gcctaaattg aatacaaagc cagtgatcat ccatgcaagt aaaggattag
    29821 aacagggaac acataaaagg atctctgaca tcttgttaga ggaaatccca gcagaaaaaa
    29881 gaaaagctgt tgttgttctt tctggaccaa gccatgcaga agaagtagca aaacatgaca
    29941 tcactacgat cacagctgca agtacagatg caaaagcagc aaaatatgta caagagctgt
    30001 tcatgaataa ttattttcgg atctatacca atcctgatgt gatcggtgta gagatggggg
    30061 cagctttgaa aaatattatt gctattggtg ctggcgctat tcacggctta gggtttggcg
    30121 ataatgcaaa ggcagcaatc atgacgcgcg gattagctga gatcagccgt ttaggtgtag
    30181 caatgggtgc caacccgctg acctttatcg gattaagcgg aataggcgat ctagtcgtta
    30241 cttgcacaag tgttcattct aggaactggc gcgcaggcaa cttgttagga caagggcagc
    30301 ccctagaaga ggtgctagcc aatatgggaa tggtcgttga aggtgtgaat actacccaag
    30361 cagcgatgga actagcccaa atgatggaaa tcgaaatgcc catcactcaa acaatctatg
    30421 aagtgctgta tgaacaaaaa gaaatcaaaa cagccgcaaa agaaatcatg ttacgtgatg
    30481 gaaaaaaaga aaatgaattc tcattaacaa ttttttgaag gagcgaatgt atatgaaagt
    30541 taaaaaagca gttattccag cagcaggatt aggaacaaga tttttaccag caacaaaagc
    30601 tatggcaaag gaaatgttgc caattgttga taaaccaaca atccagttca tcgttgaaga
    30661 agcgttgaaa tcaggtatcg aagacatctt gatcgttaca ggaaaagcta aacgaccaat
    30721 cgaagatcac tttgattcaa atattgaatt agaaacaaac ctaagtgaaa aaggaaagac
    30781 cgaattattg aaattagtag aagaaacgac agatgtcaac ttgcatttca tccgtcaatc
    30841 tcatccaaag ggtttaggac atgccgttct tcaagcaaga gcctttattg gaaatgaacc
    30901 atttgtcgtt atgctaggcg atgatataat ggaagacgaa gtaccattga cacagcaatt
    30961 gatcgatgac tatgagcgta cacacgcttc tacgattgcc gttatgcaag ttcctcacga
    31021 tgaaacttca aaatacggaa tcatcaaccc aggggaagta ctggaagatg ggttatacaa
    31081 tgtcaaaaac tttgttgaaa aaccagatcc aagcaaagca ccaagtgatt tagctattat
    31141 tgggcgttac ttattgactc cagaaatctt tgatgtatta tcagaacaac aaccaggtgc
    31201 tggaaatgag atccaactaa ctgacgcaat cgatacgttg aataaaacac aacgcgtatt
    31261 tgcccgtaaa ttcacaggta aacgttatga cgttggtgac aaatttggtt ttatgaaaac
    31321 aagtattgaa tatggtctga cacatccaga agtaggggca cctttgcgtg aatacatcat
    31381 gtctttaggt aaaacattag aagcagaagg aagcaagatc aatcataatc caaaaggaat
    31441 aacagaaatc aaagaagaaa agaaaaaata atcttttttt ctaacgagct tcagcggcta
    31501 attgaataaa aagtagttga agctcgtttt tatttgttta aaaaacacga aaatgctatc
    31561 cttatagaaa agagagacaa gaagggagtt tgatcatgtc aggaggagaa attgcaggat
    31621 tgatcgcagc aatcgccttt gcgatacttg tcgttttcat cgttcgcgta ttgttgcaag
    31681 tttccaaaac tttagaaaaa gtagaccaga ctgtcgcaga agcaaatacg acaattgaga
    31741 tcgtcacaca agacgtagat cttttatcta gacaagtaga aggcttgcta gtcaaaagta
    31801 atgagctgct cttggatatc aatggaaaag tcgcaacgat cgatccattg ttcacagcag
    31861 ttgctgattt aagtgaaagt gtatcggaac ttaatcattc tagtaaaaac atcgtgacga
    31921 aagtcggcgg aatcggaaaa acgacagcta aagcgactgt tgccggaaaa gtcggtggaa
    31981 cagcgatgaa attttttaaa aacaaaaaac atactgaaac gacaggagga aaataagaat
    32041 ggctaaaaaa ggtggatttt tattaggtgc agtagttgga ggtacagccg cagcaatcgc
    32101 cgcattattg ttagcaccca aatctggtaa agaacttcgg gatgaattgg ctgctcaagc
    32161 agatgatttg atggacatgg cttcagatta cacagatatg gcaaaagaaa aatcatctga
    32221 gttaacaggt attgcaaaag aaaaagcgca aggtctttct caacaagcag gggatctagc
    32281 aggcaatgtt aagcagaagg taagtgaatc agcagaggaa gcaggaagtg aatcagatga
    32341 tatcttgtta aacctcaaag aacaatcagc tgatttatct aatcgtttca aaaaaacagc
    32401 agaagatctg aaaaagccag cagacgaatt aggtagtatt gctaaagata cttcagaaga
    32461 tatcatgatc gatgtaaaag atgctgcaga taaagcagga gagacggtca atgaaggaat
    32521 gtcagaagca aaaccagttg cagatgacac aaaagaaatg ttgaaaatgg caaaagaaga
    32581 tgcaaaagaa gctaaaaaag aaatgaaaga tacaatgaca gattcgacta ccaatcacta
    32641 agattactca tttctagaat aaaaaaggtg agagcggacc agctctcacc ttttttattt
    32701 tcattactct aaaacaagta attcttttgg tgtagtcgtg aatggcacac aaccatcact
    32761 tgtcacatac acacaatctt cgatacgtac ccctactttt tcaggaatat agattcctgg
    32821 ttcaagagag aagcacatcc cttcttcaat cactaagtcg tttccttcta caagagaagg
    32881 gaattcgtgg acggttgttc cgattccgtg acctaaacgg tgattgaaat attcgccgta
    32941 gccatagctg ttgatcacgt ttcgtgcgat ttgatcaagt tcacctgctg tcacacctgg
    33001 gcgaactgcc tcttgtgcgg caagctgtgc ttcaagagtg atgttataga ttttttcttg
    33061 gaactcagtt ggtttttgat aagcaactgt acgagtagca tcactgcaat agccattcca
    33121 aataacgcca agatcgaata acactagttc atttggtgaa accttcgtgc ttcctggtgt
    33181 accatgaggg cttgcagcat ttgctcctgc aaggactaat gtgtcgaaag acatttgtga
    33241 aacaccttgt ttttttaatt ggtattcgat ttctgcgata attgcttgtt cagttacgcc
    33301 ttcttttact gttttgaagc caatttcaaa ggcaacatcg gcccatttac cagcttcgat
    33361 cagtttgtct ttttcttcta ccgttttgat cagttgcaga cgctggataa tagaagttag
    33421 atcggtgtaa gaatctgtac ctgtcaagat atcagagata gcatcgaagc gatcgacagt
    33481 cagagcattc ttttcaatac ctacattacc aggttgccca tagcgtgcgc gaagctcttt
    33541 tccgatgatc tcccaaggat tttcgctgtc taagtagcca cgtacatcgt acgcccagct
    33601 gctgttttga gcatcttcaa cttccagagc aggagtgaaa aggaaaggat cttgttccag
    33661 agggataaaa agagctagta cacgctcgtg aggatcactt tcatatccgg aaaagtaagc
    33721 gatgtggcca gggtcgctga tataagccaa ttctacttgg tgttgtgcca tccattgacg
    33781 tagttcattg atttttacgt tattcatttg gcaacctact ttctaaatta attttgtatt
    33841 caataattgt accactgatt gattaaaaga ccaacgttct taaaactgaa aaaagtaaaa
    33901 attttcatga aaacagagat aaaacggttg caattgttga gattatttga tatcctattt
    33961 tagaaatgta agacgtattt tcagaaaaca tataaaatat gaataggaga acacaaatgg
    34021 aaaagcaaac aatcactatc tatgatgtag cgagagaggc aaatgtttcg atggctactg
    34081 tttcacgtgt cgtaaatggc aatccaaacg tcaaaccagc aacaagaaaa aaagtcttgg
    34141 aagttatcga tcgcttggat tatcgtccaa atgcagtcgc acgtggcttg gcaagtaaga
    34201 aaacaacaac agttggggta atcattcctg atgtcagcaa tatgttcttt gcctctttag
    34261 cacgtggtat cgacgatgtg gcaacaatgt acaaatacaa tatcatctta gcaaactcag
    34321 atggcaacga tcaaaaagaa gtcaatgtat tgaataattt attggctaag caagtggacg
    34381 gcgtgatctt tatgggtcac catatcacag acgaaatccg tggagaattt tcacgttcaa
    34441 aaacgcctgt tgtactagct ggttccattg atccagatga acaagtaggt agcgtaaaca
    34501 ttgattatgt gtcagcaaca aaagatgcag taaataaatt agcaaaaagc ggcaataaaa
    34561 aaatcgcttt tgtcagcggt gcgctgattg atcctatcaa tggtcaaaac cgtctaaaag
    34621 gctataaaga agcattaagc gaaaacaatc taccatatag cgaaggattg atttttgagg
    34681 cacaatacaa ctttaaagat ggtctgtctc tagttgaccg gatccataat agcggtgcaa
    34741 cagctgtcta tgtttcagac gatgaattag ccattggtat cttggatggt ttattagacc
    34801 gcggcgtaaa agtaccagaa gaattcgaaa tcatcacaag caacaattct ttactgacag
    34861 aagtagctcg tcctcgtttg acaagtatca cacaaccatt atacgattta ggagcggtat
    34921 ccatgcgcct attgacgaaa atgatgaata aagaagaagt cgaagaaaaa acgattatct
    34981 tgccttatgg aatcgaagaa aaaggatcaa ctaaataata aaagatcaat catttaaagg
    35041 ttgttccaaa agtggtcaac ttctggaaca acgtttatac gagaaagggg gttatgacaa
    35101 aattttgtca taaccccttt ttttttgtga ctttaaaaaa agagaaaatg gcggaaacaa
    35161 accttacgtc catcgtatac gacacaatct gtgcgtagat gctacatttt gttcactact
    35221 atcaaaatta ctttaaaatg aaaacgtgat aaaaaagaaa gtagaaaagt agataagtaa
    35281 ctctaaaaaa tccaaaaaaa tccaggctcg attgcgtact tatcttaaag gagaaacaat
    35341 ccatcatata taacccgaaa ggagacttct atttgcctaa gaaaagacga aagaccatag
    35401 tcatcatttt gaccattctt actataggaa tcggagggaa attttacatg gacaaaagag
    35461 aaatgcaaag acagaaagat ttactcgcag tagaaaaaca gtccgtcaaa gttctgaaaa
    35521 atacctttgc ggatatcaag gaagtgaaga ttgaaaaatc tgctcgaaat gaaatgacgg
    35581 gttcataccg gatagttatt ctaatgacta ataaacaaga tcaatcgatt tatttttctt
    35641 atagtttttg gaaagaaaga aatgagatag gaagttacgg aatagttgat gaaaaaaagc
    35701 aaaaagaagg aaatacgctc aataaagtta aagtaactta ctctaatgga aatgaggaaa
    35761 gtatttaatg attactgaca aacaatatag tgaattaagt gatgctgttt actggctaga
    35821 tcctaaacat agagactatg tacctgaaat gcaagaaaat ttaagtttta aaattaacgg
    35881 cactatgtat aaaatcctaa aaatcaaaaa ctccttcgac ggcatgcagg cgatggctgt
    35941 agcgccaatc gtccatagca aattggagaa aaatttcaaa aataaaaaaa ttcctgcaaa
    36001 ctttcgcgta cttaaataat gaaggtgtca atacttacat aaaaagaaag gaggcacgtt
    36061 cttgtcaagg aaaataagaa aactcagctt agccatttta ttaatcgtca tattagggat
    36121 cggagggaaa gtctatatgg acaaaagaga agcacaaagg cagaaagatt tactcgcagt
    36181 agaaaaacag tcagtcaaag ttctgaaaaa tacctttgcg gatatcaggg aagtgaaggt
    36241 agaagaattt aaaaaaaacc ctgttaccgg aagttatggt gcacttgtga caatgacaaa
    36301 taatgaaggg aaatctgttt attttgatta tagtttttgg aaggagagaa atgaacttgg
    36361 tggttttggc attgagaaca gagcggtaca aaagacagga gtcactataa aaagagtaaa
    36421 agtaattttt tctaacggac aggaggagtt agtatgacaa taaacgataa gcattacaat
    36481 gatattagtg agaaggttta ttggctagat cccaaatatc cccgctacaa tgagggatac
    36541 aaaaagaact ctgttaaaga atttgctggt atggaatttc aaatcctaca aatcaaagac
    36601 tcattggacg gcatgcaggc gatgtctgta gcgccaatca agaacggtaa agtggatctt
    36661 tctcaagtag tgattgcttt tgcaggaacg aacttaagtg attggaaaga tttggaaacg
    36721 gatgcaagaa gtatcatgaa tctctacggg aatagaccag gagcaagttt ttctgaaaaa
    36781 gcaacaacaa acaagatttt atccgttagt ggattgaaaa cagcggggca aatcaattca
    36841 gcgattgcat ttgctaatga agtaaaaaag gaatatggag aagctgctat ttctactacc
    36901 ggccattcat taggtggatt cttagcatta tatgtagcag cagagaaaca atggaaaaat
    36961 gtcggcttca acgggccgga cccgtatgat ctcttatcac cagaagcaaa aaaatgggtg
    37021 gaagaaaatc ccggtatgct caccaattac cgaaatcacg ccgattttat cggtaatttt
    37081 ggtggtaatg gcacaggtgc tgaagttcgg atcagtatgg aaacagcatt cttgaatctg
    37141 cttgcttccc acagtttaac ctcttggaag tttgatgaaa atggaaagct gattattcct
    37201 gaaaatgaaa acaatgttaa agctcgaaaa caacagaaag aaaaccaagc gacccagttg
    37261 ttttcctatc agttgcaaga actgaacaac atacgtaaaa aactacgatt aagtggcggc
    37321 cagctgacag ttaatgaaca aatttactta gatgacacac aagctaggtt agtagtggaa
    37381 aagttatcgg caaaaatgaa aagcgctatg gaagaagtta tccgcatcaa tcaaagagga
    37441 atttttgagc tagagaaaaa atggcaagaa ggcttgcggc aagcacgtgt tgtagcccct
    37501 tcgttgactg aacaggaaat catagaagca ttagctagtg tgggcgccac aaaagaaaac
    37561 ttagtcgatt ctgcgaaaga agtctatctg ctacgaatca ataaggcaag acgtatgggg
    37621 gaggcctttg atacattggc gaaggagatc caagacaaaa tcaatgaaat cgtggcacga
    37681 gatcgagaat tagcacagca attcaattaa aggagaagta gatgaaacta gaagaattgg
    37741 aaagagatta tcgaagtgaa cgaagacaat tagaagctca agaagatgaa ttgatccact
    37801 ttaagaatca aggattacga ttgatggagc aagaggagca gcgactgcat catgtattaa
    37861 agaacttagc tttagataat gagcctctgt ttcaagcaag acgaagttat tcgaattatc
    37921 aggagcatta tcaacaagcc ctacaagtag aacgacgaaa actctatcag cagcaagatg
    37981 aactagaacg ttcctatcgt caaaaaatca gacaaataac tatggagaaa tcatcttatg
    38041 aatgaatttt taatgcaatt actaaaagaa ttagagaaaa atctacatcg atcaaatgta
    38101 cgttcagttg gtatggggat atctcagcaa atttgggcag aaaaacgatt gacagatcaa
    38161 aactaccaaa tgactgcaga agaagctgct caagaatttt ctaaccgaca tgtacacttg
    38221 aaaggatcag taaaagggca atttgcagaa aaactaactg aattattcca aagacaagaa
    38281 aagaaattaa aacagtttcg ttagtaaagt tatatagaaa aattttgtga aaat
//
LOCUS       DAFBQH010000023        34677 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.023, whole genome shotgun sequence.
ACCESSION   DAFBQH010000023 DAFBQH010000000
VERSION     DAFBQH010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.023"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            3..362
                     /locus_tag="LK694_001187"
     CDS             3..362
                     /locus_tag="LK694_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052070.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            534..1541
                     /locus_tag="LK694_001188"
     CDS             534..1541
                     /locus_tag="LK694_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK5052071.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            1566..2750
                     /locus_tag="LK694_001189"
     CDS             1566..2750
                     /locus_tag="LK694_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBK5052072.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(3187..4770)
                     /locus_tag="LK694_001190"
     CDS             complement(3187..4770)
                     /locus_tag="LK694_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK5052073.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(5348..5491)
                     /locus_tag="LK694_001191"
     CDS             complement(5348..5491)
                     /locus_tag="LK694_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBK5052074.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(5713..6192)
                     /locus_tag="LK694_001192"
     CDS             complement(5713..6192)
                     /locus_tag="LK694_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK5052075.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(6265..6549)
                     /locus_tag="LK694_001193"
     CDS             complement(6265..6549)
                     /locus_tag="LK694_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052076.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(6627..6821)
                     /gene="ssrS"
                     /locus_tag="LK694_001194"
     ncRNA           complement(6627..6821)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="LK694_001194"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(6893..8179)
                     /locus_tag="LK694_001195"
     CDS             complement(6893..8179)
                     /locus_tag="LK694_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBK5052077.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            8317..8988
                     /locus_tag="LK694_001196"
     CDS             8317..8988
                     /locus_tag="LK694_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBK5052078.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            8954..9442
                     /locus_tag="LK694_001197"
     CDS             8954..9442
                     /locus_tag="LK694_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBK5052079.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            9455..10402
                     /gene="prmA"
                     /locus_tag="LK694_001198"
     CDS             9455..10402
                     /gene="prmA"
                     /locus_tag="LK694_001198"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBK5052080.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            10403..11167
                     /locus_tag="LK694_001199"
     CDS             10403..11167
                     /locus_tag="LK694_001199"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBK5052081.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITKITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            11400..13613
                     /locus_tag="LK694_001200"
     CDS             11400..13613
                     /locus_tag="LK694_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBK5052082.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            13624..14070
                     /locus_tag="LK694_001201"
     CDS             13624..14070
                     /locus_tag="LK694_001201"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBK5052083.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            14241..15500
                     /locus_tag="LK694_001202"
     CDS             14241..15500
                     /locus_tag="LK694_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBK5052084.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(15589..16536)
                     /locus_tag="LK694_001203"
     CDS             complement(15589..16536)
                     /locus_tag="LK694_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK5052085.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            16930..18222
                     /locus_tag="LK694_001204"
     CDS             16930..18222
                     /locus_tag="LK694_001204"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBK5052086.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            18237..20006
                     /gene="aspS"
                     /locus_tag="LK694_001205"
     CDS             18237..20006
                     /gene="aspS"
                     /locus_tag="LK694_001205"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBK5052087.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            20115..20990
                     /locus_tag="LK694_001206"
     CDS             20115..20990
                     /locus_tag="LK694_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK5052088.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            21256..23139
                     /locus_tag="LK694_001207"
     CDS             21256..23139
                     /locus_tag="LK694_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBK5052089.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(23214..23498)
                     /locus_tag="LK694_001208"
     CDS             complement(23214..23498)
                     /locus_tag="LK694_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBK5052090.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(23488..24222)
                     /locus_tag="LK694_001209"
     CDS             complement(23488..24222)
                     /locus_tag="LK694_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBK5052091.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            24351..24974
                     /locus_tag="LK694_001210"
     CDS             24351..24974
                     /locus_tag="LK694_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBK5052092.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            25166..26287
                     /locus_tag="LK694_001211"
     CDS             25166..26287
                     /locus_tag="LK694_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBK5052093.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            26294..29422
                     /locus_tag="LK694_001212"
     CDS             26294..29422
                     /locus_tag="LK694_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBK5052094.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            29441..32635
                     /locus_tag="LK694_001213"
     CDS             29441..32635
                     /locus_tag="LK694_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBK5052095.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(32781..33488)
                     /locus_tag="LK694_001214"
     CDS             complement(32781..33488)
                     /locus_tag="LK694_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBK5052096.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            34253..34651
                     /gene="spxA"
                     /locus_tag="LK694_001215"
     CDS             34253..34651
                     /gene="spxA"
                     /locus_tag="LK694_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBK5052097.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        11392 a         6184 c         7311 g         9790 t
ORIGIN      
        1 atatgccata tggacaaaaa acgatcagta gaaaatgtta taagggctct attttatttt
       61 ctgtcttgct tttatttctt ttatgtacgt ttttattgat ggcagcatta gaaagctacc
      121 gtttgtcaat ggatcttaat atacgaatca aaaattatta tatagcaaaa ataatgaagc
      181 agatggcttt atcggaacag tctacgcttg ccgaaagttc ggaaggagtt ttttactaca
      241 caactggatc tgtaacatat cagtgggtac aacagtccct attcttagag gtggaagtct
      301 caccgtttat ctttcggttc acagaagaag taaaaaatga tacagatact agtacagaat
      361 gatggaatgt tcgtgaaaac gccctctttt ctgttataac actgttttta ataaaaaaat
      421 gtttgccgtt tcacaaataa aaaacagtga aaacgattga aattttgtca attgttcgat
      481 agaattaaac aggtgaaaaa attgttataa tatatctaga aatgaggtgc accatgttac
      541 cagaaaaaat ggaacaaggg tttaatcaaa acctggaagc tattcagctt ttacagaatg
      601 cattgggaac ttccttttta gaagcctatg tcgaaaatgc agaaaattta atagatgatt
      661 atcaagtacg tgtggtggat ggagtgccga caaaagagac aacgcaacgg atcacagctc
      721 tttatgaaga gttgaagaaa ctttctttcg agccagaaga atggcgcaga ctgtctcaat
      781 tacttttatt gaagggcagc cagacagaac atttgcaggc gaaccatcaa ctcactcctg
      841 atagtatcgg ttttttattt gtttttctta ttgaacaact atacacaaat aaaaaagcac
      901 cggttaagat tctggatatt gcggcaggaa tgggcaactt gttattaaca gttcttttaa
      961 acctatcgaa tgcggggtac caaacagaag gaattggtgt ggatattgat gatacattgt
     1021 tggctgtcgc agcttctaca agtgatttga cacaagcaaa tgttcaatac tttcatcaag
     1081 acgggttgca ggaattatta atagatcctg tcgattttgc aatcagcgat ttgccaatcg
     1141 gttattatcc gaatgacgaa aaagcaaaag agtttttaac aagtaccgaa gaaggccata
     1201 gttatgcaca tcatttattg ctagagcaat caatgaaata tgtgaagccg gatggttttg
     1261 gtttgttttt gatgccgagt ggatttttag aaacggatca aagtgaagaa atcaaaaaat
     1321 ggtttaaaga agaaggttat cttcaaggaa tgattcaatt gccagatgaa ttgttccgta
     1381 ataaacaatc gcaaaaaagt attttgatct tacagaaaaa aggaccacaa gcacaacaag
     1441 taaaagaagt cttgcttgtg aaacttgctt cattaaaaga accagagaag gtgacggaat
     1501 ttttcaacga gtttaaaaac tggaaatcat ctgctctgta atcattcaac gagaaggaga
     1561 gtcatatgtc taaaacaatt gcaatcaatg ctgggagttc aagtttgaaa tggcaattat
     1621 atcaaatgcc agaagaaacc gtagttgcga aagggatcgt cgaacgtatc ggtttacaag
     1681 attctatttt taccatcaag tacggcgata atcaaaaatt cgaacaaatc atggatatcg
     1741 aaaaccacga agtagcagta aaaatgctgt tagatcagtt gattgaactg caaattttaa
     1801 gctcttatga tgaaatcacc ggagtagggc accgtgttgt tcacggagga gaaacttatg
     1861 gggattcagt agtgatcgat gagaaagtaa tggaccgcat tgctgaatta gcagaatttg
     1921 ctcctttgca taatcctgca aacttaatgg ggatcaaagc gtttaaaaag attttacctg
     1981 acattttgag tgtggctgta ttcgatacat ctttccactc aacaatgcca aagcaaaatt
     2041 atttatatag cttaccggtg tcttattatg aagatttcaa agtaagaaaa tatggcttcc
     2101 atggcacaag tcatcgttat gtgtcggaac gtgcggcaaa attattgaac aaaccgatcg
     2161 aagaactaaa aatcatcact tgccacttag gaaatggtgt atcgatcaca gcagtcgatg
     2221 gaggtaaatc catggatact tctatgggat tcacaccatt agctggggtg acaatgggta
     2281 cacgttcagg tgatatcgat ccagctgttc tcccatattt aatggaaaaa ttagagattg
     2341 atattgatga aatgatcaat gttttgaaca agaaatctgg tctgctaggc ctaacaggat
     2401 tgtctagtga tatgcgtgat cttgaaaaga actatgaaaa agaacatatt cgtttagctt
     2461 atgacatttt cgtggaccgc atccgaaaat acatcggcgg ttatgtgaca acgatgaatg
     2521 gtgttgatgc aattgtcttt actgctggta tcggcgagaa tgacggacat gtacgtagtg
     2581 aaatcatcaa aggtatgtct tggtttggtt gtgaaatcga tccagcgttg aatgaattgc
     2641 gtggtgaaga agtagatatc tctacaaaag aatcaaaagt aagagtatta gtgatcccaa
     2701 cagatgagga actaatgatt gcccgtgatg tggagcgtct gcgcggctaa gccaaagcat
     2761 tcgttaggat aaaagatgaa acgcatgcaa gagggaattc ttgcatgtgt tttttttgcg
     2821 gagaagaccg caaaatggta atttaacttc aatttccctt cttttttaca ggatcttctt
     2881 agggaatttt gcttggaaaa gtggtcaaaa gggaaaagac tggcatttta tttattgatt
     2941 ttcaggaaaa tcccaatatt ttattttcat tcatctttat aagggacatt acatatgtaa
     3001 aaagaaaaaa catttttaga tcagcctcgt gggctgtcga ctttattctt cgctttatgt
     3061 ttagctagaa aaccgatccg aaacattcat tgatcgaata ataaaaactt gattttccac
     3121 ctgtactttg cctgaatcgt gaaagcacag gtatgaaaat caagtttttt gttgcgaaaa
     3181 aacgtcctag tcaatcagtt ggccgagcgg ctggcggctg atctgctcct gatcaataac
     3241 gagtgcttct tggaagtgtt gctgcaaaaa gtagcggtca tggctgactg ctaggagcgt
     3301 tccttgatag tcttgcagca tttcttcaat ttcttctcga gcatagctgt cgaggtgatt
     3361 ggttggttca tccaaaatca ggacgttgat tttttctaaa aagagtttca gcaacgccaa
     3421 acggattttt tcaccaccgg ataaatcttt gatccgttta cccacatcgc tttgataaaa
     3481 gccacttttg gctaattgcc gtcgagcgac ttcttcgttt ccagtcaatg tgatggcgta
     3541 agccagcaaa cggctatcgg gatcggcaaa ctgcagctgc tgcggcaaat aaccgatttg
     3601 caagctacta cctaatttga tggtgccacc gtaaggcttt tcctcaccga gtaataattt
     3661 gagtaaggtt gattttcctg caccattgtc gccgatgatg gctaaatgat ctccacgata
     3721 gactgcaaaa ctgctgtctt tgaatagtac ttgatcagcg tactgctggt gcaagttttg
     3781 aatgatgaaa acttctttgc cagattttcc accattagac aagctttgca gccgattttt
     3841 cgggggaggt ggcggtttga cgagtgtcag tttcgccagg cggcgttcca gttctttggc
     3901 ttttttgaag aaggactcat tgtctccttc atgtgcccac tgacgaaaac gacgaatcat
     3961 gactttcaac cgctggactt ctttttgctg cagttcgtaa ttttttgtca gctcagccaa
     4021 gcgagcttct ttcaagactc gaaaacggct gtagtttccg ggatactcaa tcaatgcacc
     4081 ttcttccagt tcccatgttt ttgtggtgac ttgatcgaga aattgccgat catgagaaac
     4141 gaccagaacc gatttttttg tattctgtaa atagttttcc aaccagcgaa tactgttaag
     4201 atctaaatga ttggtcggtt catcgagtaa aagtaaatcg gcatcggctg ctaaaatttt
     4261 ggctaaagcg actttaactc gttcgcctcc gctaagttct gttaaccgtg cttcctgacg
     4321 gtgtggaatc cctaagcctt gcaagatcgc tgcaatccgg tcttctaata cgtaaccgcc
     4381 tagttcttca aagcgatgct gcagctcacc gtaacgggcc agacacttct ctaagtttgt
     4441 atctggttct gtcatcaatg cttcgtagcg actcatttgc tgtttgaggt cctgtatctc
     4501 agcggcagaa tggagcaagt agtggttgac ggtcgcttcg gaaactgtaa gttcttgttc
     4561 gaccgtcccg atctttaagc cttttttgcg actgataaca ccgctgtcga caccttctct
     4621 gccggtcaag atttgtaaaa aggttgattt gccagttccg ttgataccga ctaaggcaat
     4681 tttttcacca gcttcaattt gacaggataa atcctcaaat aatggattcc cgccgattgt
     4741 ttttttaata gaaattgctt gtatgatcat gatctctctc cttgagacca ctcaaaaagg
     4801 ccatggttat ccctggcctg tgttatttaa actcagatta tcatgagaaa aataccttga
     4861 gatggccatt gattttacgt gaattcaact gttttggaca aacttgtccc gtcaacagca
     4921 actgtaaaac caattgcatg gctaaatgcg atatggtatc gttggtaatt ttcattactt
     4981 gttattttag tcatgcgcat catagtattt tccctccttc gtgtagttgt cttttccagt
     5041 gtacctgaaa gtgtcagaaa gttcaactag cgctgacatt tacttaatat ttatcgatgt
     5101 gtaattctgt cagagagctt tttttatctg actgagggta atgtctcgtg gaccacatca
     5161 ttgtattctg agctaaacca gcgttcaggc gaaatcatat ggtgtttgat aaaaaacttt
     5221 gtctacatga cttccttttg ccatttgctt aataccgttg cattaaacag ctcttctctt
     5281 tggaaatctc accaacatcg cgttcaggag tatcgttgtg gttgcaatgg caagcggtag
     5341 cctttcatca attcttgaat gatttaccga atgtagcact tgccttctcg cggtacccga
     5401 acccgaaata aaattttagt ttggcgataa aatcggtatc cgccatcgat acttcatatt
     5461 ttttcgtatc agattcatcg gaaacttcca cattgacgaa ccaataagct ttggcatgct
     5521 taggtcgttt taaaccacac cagggcgact aatcgccctg gtgttcactt tttttatttt
     5581 acttgaactc attgactgtt ttccctttat tttaaacatt ataaagaccg atgagtgctt
     5641 gccgcgtctt ttgtttttat cacggttttt tgtgcaaaaa aacctgccga tagttggcag
     5701 gtcgtctacg tgttaagctt tggtgtggtc tttagctggc aattggtttt ctaaatcagt
     5761 tcggacaaca agcacatcgc aagctgcgtt tctaataaca tattcagaaa cagaaccaat
     5821 aaataaacgt tctacagcat tcaatcctgt ggctcctaac ataatcaaat ctacttgatt
     5881 atcttctggg atttctctgg caataatttg ttttggtgag ccatattcta cgacagaggt
     5941 tacattattc aagccagctt tcttcgcatt gctttcataa tctgccaatg tttgctttgc
     6001 catttcagta gcttgttctg caagcattcc atcaaaggat gaaacggttt ggaaagaacg
     6061 tgtgtcaatg acatgtgcta atagaagttc tccgttattt cggatagcta cattgaccgc
     6121 ctttttaaaa gctaattcag cttcatcaga accatctacg gcaaccatga tttttttgta
     6181 ttgttgtaac ataagcattc actcctcaag tcaatcgtat tgtactcttt tattgtagaa
     6241 cactttaggg aaaaagagaa ttatttactg ctttggttca gcaatttttt ttgcaaatcg
     6301 gatactgaag gtggcagaaa caataattac gatcagtaaa aagaccaagg caataaagcg
     6361 ttgattgaaa aaagctgtta aaatacccat tacgcctaaa acagcatatg cagcaccgta
     6421 aaattgtagg aatccttgat ttttatcatt tttgttgatc aatacaaaaa agatatcctg
     6481 tttattaaat aaataatagc taacgaataa taatactaaa gtggcgataa tcattaaaat
     6541 cctaatcatg aacaaatcct ccaaaataag caacaaatag aaaaagcaat ttgccaagag
     6601 caaattgctt tattctttaa aagatggtaa tctccgttaa tgccgttgtt tgtcctgtag
     6661 tattgatccc gcgaattcaa gcaggtgggt cccatggtcg tagcttcgaa ctaccaaatg
     6721 ataaggcatg ttaccagcag tgacacaaga cgggtcccaa gatatcaata aaaatgttcg
     6781 gtcaacacac aaagaggaca attcgaacat cacagatttc ccacctctat agtagcatat
     6841 gaatttaaaa agagcaatga gagagctcgc agaaacagaa ccgttttctt gtctagtttt
     6901 ttggaggatg ctttttccac tcttttatac ggtaatactg ttgacccaga gcctgttcat
     6961 atttccctgt atcttttggc tgataatact gagcattctt cagtttatct ggtaaatatt
     7021 gctgattgac ccaagcctga tcaaaatgat gaggatactg ataacccacg ccacggttga
     7081 gtgatttcgc acctttgtaa tggctatctc tcaaatgatc aggaacatct ccagctttgc
     7141 cctctcgaat atcagcaagt gctgcatcta atgccatgta ggcagaattg gattttggcg
     7201 acaagcaaag atcgacgacg acgtctgcta gtgggatacg tgcctcaggc aatcccaatt
     7261 tttcagcagc taatacagcg ttcactgtac gagccgcagc agcaggatta cctaaaccta
     7321 tatcttcata accgataacc atcagacgcc ggcaaatact tgccagatca cctgcctcaa
     7381 ctaaacgggc gagataatga agtgcagcat ccacatcact tccacgaatc gatttttgaa
     7441 aagccgaaat aacgtcataa tgagcatctc catttttgtc atgggtaaga gcttttcttt
     7501 ggacacattc ttcaatgatc gacagtgtaa gatgaatcct tccttcttta tctgaaggag
     7561 tcgatagtgt agccagttcc agcccattca atgcactccg caaatcgccg ttcgttgcac
     7621 gcgaaaggtg aagaagagca tcttcgtcta actggattgc ttgttgtcca aggccgcgtt
     7681 ctttatccct taaagcatgt tcgactgcca actggatgtc ttgttctgtt aatggtttga
     7741 cttcaaagat ctgtgtacgg ctgcggatcg ctggattgat ggtaatataa ggattctcag
     7801 tagtcgctcc gatcaagatg attcggccac tttctaaatg aggaagcaga aaatcctgtt
     7861 ttgttttatc taatcggtgt acttcatcca ataagaggat cactgtacca ctcatttttg
     7921 cttcttccgc tacaacttgt agatcctttt ttgaatctgt agcggcgttt agcatgcgaa
     7981 atgcataatt agtagagccg gcgatcgcac ttgcgatact cgtttttcca gtaccaggag
     8041 gtccatatag aatcatggaa gagagcatgc gagcatcgac catgcggcgt atgatttttc
     8101 cttccccgac tagatgctgc tgaccaacga cttcatcgat cgttcgaggt ctcatgcgat
     8161 aagccaatgg ctgttgcata ggattttcct ccttcttgtt tttacgtccc ttaattatat
     8221 catatttagc aagatgccta gaaagaacag ccttctgttg ttttgttttc atagcttatc
     8281 caactaatat tgtgtatgat aaagtaggtg agaaaaatga cggataaaca aaagatagat
     8341 gaattttttt ggaataaaac gacgccagaa gtggcacggg atttattggg gatgtatttg
     8401 gagcatgaca caccagaagg aagattagct ggatatattg tagacgcgga agcataccta
     8461 ggtccagaag acgaggccgc ccatagctat ggtttaagaa gaacaccgcg tgtgcgggca
     8521 atgtatgaaa aaccaggtac gatctacctt tacacgatgc atactcatcg gattttgaac
     8581 atcattactc agccagaagg gattcctcaa ggagtaatga tacgtgcaat cgaacccgcc
     8641 gcaatgattg atcaaatgag taaaaatcgt ggcggaaaaa ctggtccaga tatcagcaac
     8701 ggtccgggaa aattagtgga agcattagca attcctcaag aactttatgg tcagtctata
     8761 gcagatagtt cgttacgctt agtttttgag aaaaaaaaga caccaaaaaa gatcaatgct
     8821 ttgcctagaa tcggtatccc aaacaaaggt gtatggacag aaaagccgtt gcgttttgtt
     8881 gtgtcgggaa atccttatat ctctttacaa agaaaaaacc aaatcgagaa agattggggt
     8941 tggaggaaag aaaatgaaaa agaaggatct attaacatat ttagatgaaa taattgaaaa
     9001 acaagtgaca gactatgatg tagctttaga ttgggataca aaaaatcata cgatcgagat
     9061 cgttgttcgc ttgtttgcag aaaataaagc acaatttgag attgatgatg cagaagggat
     9121 cgtttcgcaa gaaccaatta tcgaatttga agacggcgta cttcttttta atcctcagaa
     9181 atctgttttt gatgaacgca attatctagc agtaatccct tatgaaggaa aaaaagggtt
     9241 ggcaaaatca gtagctgatt ctttagtgga atatctaaat gaagtattag ctcaagggca
     9301 aagcgatttg cttgattttt tggatgaaga cgatgacgaa gctgtattcg agcttcactg
     9361 ggataatcaa caactagcag aattagtaga agtaaaacag caaaatgaag cagatactta
     9421 tttaccgtac ccaagttatt aaggagtgac agatatgaaa tggaatgaag taaaagtaga
     9481 aactgaaagt gaagcagtcg aagcagttgc taatattttg atggaggcag gggcgagtgg
     9541 tgtcgcgatt gaagattctt tagatgttga acattttcag tcggatcaat atggtgagat
     9601 tttagataaa aatgatttta catcgctgaa agaaggcgca ctagtcatgg cttattttcc
     9661 ggaaacgact ttcttaccgg aaatcttgcc ttttatcaaa gatcgtatca cacaattacc
     9721 tgagtttggt ctagctattg gaaaaaataa agtaacagtt agtgaagtag aggaaagcaa
     9781 ctgggcaaca gcctggaaga aatactatca tccggtgcgt attacgcgaa tgctgacggt
     9841 tgcgccaagc tgggaaaact accagacaga agatcctcta gaaaaaatta tcacactaga
     9901 tcctggtatg gcatttggca caggcactca tcccacgact tccttgacat tgacagcact
     9961 agaaacagtc ttgcgtggtg gtgaaacatt gctagatgtg ggaactgggt cgggcgtgct
    10021 aagtattgca gcaaaatatc ttggtgcaaa agatgtctat gcctttgatt tagacgaagt
    10081 agccgttcgt tcggcgaaag aaaatatgga tatgaatgaa atagccaaag atgtccatgt
    10141 atcagctaac gatcttttga caggaatcga gattgaaagt gatgtaatcg ttgcgaacat
    10201 tttagcagat atcatcttat tgatgatccc agatgcttgg cgtttactga agcaaacagg
    10261 cacgctgatc gtttcaggaa tcattgaaga gaagaagcaa atggttctgg atgccatgtt
    10321 agaacaaggg tttatcgtgg atcaaatttt ccagcaaaaa gactggtatg caattatttt
    10381 gaaaaaacca gaggtggaat agatgcagcg gtatttttta aaagaatcat atgaatcaaa
    10441 agactatttt gttttagaag gggaaaacta tcaccacatt gtacgggtta tgaggatgga
    10501 acccgaggat gaagtttttt tggcattcaa tgatcaaata gccattgttg cagaaatcac
    10561 gaagattact gaagaggctg tttatctgaa agaaattaaa aaagaagaaa atcaaaagga
    10621 actgccaagt tcgattacca tagcgagtgg ctatcctaaa ggggaaaagc tggattggat
    10681 cgttcagaaa tccactgaat taggagcgca agcttttctt ggtttcccgg catcagcctc
    10741 tgtcgttaaa tgggatgcaa aaaaacgtat gaaacgacaa caacggcttg aaaagattgc
    10801 tcaagaggca gcggaacaat cccatcggca gatcgtgcca tcagtagagt tattggaaac
    10861 gaaacaagat ttatttgagc ggctagatga ttatgacgtg gtcttggttg cttatgagga
    10921 atcagcaaag caaggcgaaa aatcaaatgc agccaaaatt cttagtgaaa caaaaccaga
    10981 tagtcgaatc ttagctgtct ttggaccaga aggtggatta tcccgcgatg aagtcgatta
    11041 ttttgtagaa cacaaaggga tactttgtgg attaggacca agaatccttc gtacagaaac
    11101 agcacctctt tatttattaa gtgctgctag ctatcaatgg gaattaatga atccttcttt
    11161 cagctaacgt tcttcattaa taaaagagta tcgacagaaa gaattgaaaa aatagtcaac
    11221 aatttgtata atagaaacaa ttatttatac aatggatacg aaaaatcgtt tgaaaaagct
    11281 tttaaaaagc gaacatttcc gtaaaaaaac gtataactgc acggattttg ggacaattat
    11341 caaacagata tttgtcccga ttcccgtttt accgaaaaaa gaaaggggga tcgattcctg
    11401 tgacaaaaga agaaatttta acgggacccg gtgtcataaa gctcgttagt caatacatgg
    11461 gtcccgaaca tgtagcattt gttgagaaag catgcgaata cgcgactgct gcacatgacg
    11521 gacagtttag aaaatcaggc gaaccctaca ttattcatcc tatccaagtc gcaggtatat
    11581 tagcagattt aaaaatggat ccccatacag tggctacagg cttcttacat gatgttgttg
    11641 aagatacaga aatcacttta gaagatctga gagaagaatt tggcgatgac gttgctatgt
    11701 tagtggacgg cgtaaccaaa ttagggaaaa taaaatataa atcccacgaa gagcagctag
    11761 cagaaaacca ccgaaagatg ctgctagcaa tggctcaaga tttacgagtt atcatggtca
    11821 aattagctga tagactacat aacatgcgta cgttgaagca cctgcgagaa gataagcaaa
    11881 gaaggatcgc tcaggaaact ttagaaatct atgcgcctct tgcacatcgt ctagggatca
    11941 gccggatcaa atgggaatta gaagatacag cacttcgtta tctaaatcca aaacagtatt
    12001 accgtatcgt ccacttgatg cagacgaaga gagaagaacg tgaaaaatac gtaagtggta
    12061 ctgttgaaga tattcgaata gcgacagaag agctggggat ttttgcagaa atctatggac
    12121 ggccaaaaca catttattca atctatcgta aaatgaaaga tcagaaaaag cagttcaacg
    12181 aaatttatga cttgctagct attcgagtga tcgtagattc aatcaaggat tgttatgctg
    12241 tattaggagc aatccacaca aaatggaaac caatgcccgg cagatttaag gattatattg
    12301 ccatgccaaa agctaatatg taccaatctt tgcatactac cgtgatcggt ccggcaggaa
    12361 atccggtaga aattcaaatc cgaacacagg aaatgcatga aatcgctgaa ttcggggttg
    12421 ctgcacactg ggcctataaa gaaggaaaaa atgaaaaagt agaaccagat ggtatgacga
    12481 aacaattaag ctggttccat gagatactcg aacttcaaga cgaaagctat gatgcttctg
    12541 aatttatgga aggcgtaaaa ggagatatct ttagtgataa agtctacgtc ttcacaccaa
    12601 aaggagacgt tactgagtta ccaaaaggat ccggaccatt ggactttgca tacagtatcc
    12661 atacagatat cggtaacaaa accactggtg caaaagtaaa tggcaaaatg gtgcagcttg
    12721 attacaaatt gaaaaacgga gatatcattg agatcatgac ttctccaaat tcatttggcc
    12781 caagtcgcga ctggttgaaa ttagttgcta ctagcaaagc aagaaataag atcaaacgtt
    12841 tcttcaaagc ccaagatcga gaagaaaatg tgatcaaagg ccacgaatcc gtggtcaaat
    12901 gtattacaga tctaggattt acgcctaaag atattttgac gaagaacaaa ctgcaagaag
    12961 cactcgatcg ttttaattat caaacagaag atgatctcta tgcagctgta gggtatggag
    13021 aagttagccc cttgacgatg gccaatcgtc tgactgaaaa agaacgtaaa gaacaaaaaa
    13081 tcgagcagca aaagcaagaa gcagaagaaa tcatgaatca gccgaaaaaa gaacctgaca
    13141 aaatgaaagt acgtcatgaa ggtggcgttg tcattcaagg ggtagaaaac ttactaattc
    13201 gtatcagtcg ctgctgtaat ccgattcctg gtgatgatat cgttggttat atcactaaag
    13261 gcagagggat atccattcat cgtcgagatt gtccgaatgt tcagcctgac aaaccaaatg
    13321 tagcagaacg tttgattgaa gtcgaatggg aagatacatc gaatacacga aaagagtatg
    13381 atgcagattt ggaaatttac ggctataatc gttcaggctt attgaatgat gtacttcaaa
    13441 cagtcaatgc gctaacgaaa aatctcaaca gcgttgaagc acggacgaat aaagataaaa
    13501 tggcgacgat ccatttgacg gttggtatcc agaatttatc ccacctaaag agtatcgtgg
    13561 ataaaatcaa agcagtacct gatgtctaca gtgtacgccg gacgaatgga taggaggaca
    13621 gatatgagag cagttgttca acgggtaagt aaagctgctg tgtcagtcga tcagcaagaa
    13681 attggaaaaa ttggctacgg cctacttgtt ttattaggca ttcatgaaac cgatacgcaa
    13741 aaagatgtgg actatctggt aaaaaaaatt gcacaaatgc gtatttttga agatgaacaa
    13801 ggaaaaatga atattagtgt agaagatgtg ggaggacaaa ttttaagtat ctctcaattc
    13861 acactgtttg cggatacgaa aaaaggaaat cgaccaagct ttgtggaagc cgcacgacca
    13921 gaaacagcta ttcctttata cgaatcattc aatgaaggaa tcagaagtcg cggcatcaca
    13981 gtcgctaccg gacaattcgg agcagatatg tcggtttctc tcgtcaatga tggacctgta
    14041 acgatcatta ttgattctaa aaacaaataa agtaatctgg ttttcactag taaaaactat
    14101 gaaacacgtc gcttaagtta gactgataaa ctctaactta agtggctttt ttgtctgcaa
    14161 aaagcgcttc ttttgtcagc agtcggaaaa aaagttaagc ttaaattata caagttattt
    14221 tttataggag gttgctgaag atgaaagaag taccagtagt agatatcaaa atcacagatt
    14281 taaaaaaatt gtatcaaaaa ggatacaaca tgttggagga gttacgccat gaagcggacg
    14341 ctcccgtcgt caaagcaaag attttcaata aagaagctat cacgatctac ggatcatcag
    14401 cagcaaaagt attttatgat ccaaggaatt tcaaacgaaa aggtgctatg ccaaaattag
    14461 tattgaagac actttttggt caaggtggag tacagacttt ggatggagct gctcatcacc
    14521 atcggaaaaa tatttttatg gatctaatga caccggaaag aatggaagat tatcatcgta
    14581 tcttagataa gaatctgaca caagcacttg aagcacagca tggtcaattt gaactatttg
    14641 atttaagtaa gatggttttc tttacatcta tttgcgagtg ggcaggtatc aatttatcag
    14701 cgatttctaa agatgaggtc gaaaaattag cagaatatca aatctctatg atcagcggaa
    14761 cgtttacttc tccgattgat cacataaaag gtgtcgagaa tcgcaaaaaa tcagaaaaat
    14821 gggcgcaagg cttgattgaa gaagctcgac aaaatcctgt ggctggaaaa gaaaatgtgg
    14881 cattatatgc ttttgccaat gccacagatt tagatggtca gcttttacct ctggaagtgg
    14941 cagctgttga attattgaac atcatccgtc caacggttgc tctaactgtg tgggcggcat
    15001 tgatgggaca tgcacttttc agtagaccag atttgtatca gcaattaaaa aatgactttt
    15061 cgacgttaca agatccgttt attcaagaaa tgagacgcta ttatcctttc ttcccaatgc
    15121 tacctgcgat ttcgctgaaa gaagtagaag tagatggtta tcgtatacca gaaggcagtt
    15181 gggttatttt agatttatat ggaacagacc atgatgaacg aacagttgaa gcgccagatt
    15241 cctttatgat caaacggtat gtagggaaag caaaagatat ttcctataaa gaagaatatg
    15301 agatgattgc acaaggcggg ggaaattttc gacaaatgca tcgctgtgca ggagaatgga
    15361 tcactcttca tagtctacgt gtgttttccg atcagctagt aaataagttt gagttctctg
    15421 tacctgaaca agattggacg attccattca atcagtttcc gacttatccg aatagccgag
    15481 cgctacttta caagaattag attagcataa acttatcgaa aaaaaggaag atcaaacagt
    15541 gctgatcttc cttttttcat attactataa attgataagg tcaattgatc aaaatccacg
    15601 aaatgcttca tcaatggttt gataatcttc attggataaa tgcaaatcta aagcttggac
    15661 attgtcagat acttgctctg gacgcttagc tccaggtata actacagaca ctcgtggatt
    15721 cttcatgtac caagctaaaa caagttgcgc aggagtggct tggtatcttt tagcaatggg
    15781 gcgaagtaca tcgacagcgg ttaaaatttc tctgaaacgt tctccttgga aatctggatt
    15841 tctttttcga ggatctcctt caccaaattg tttttcctca ccaagttcat attttcctgt
    15901 cagcaaaccg gaagcaagag gaaaataggg aacaaaactg attttgtttt tttcaagata
    15961 agggaataat tctttttcag cctgccgatg gatcaaactg tatttatctt ctactacatc
    16021 gacatatcca tctgcattcg cttctttcaa ttgttctaac gtgaagtttg acacaccgac
    16081 tgctcggatt tttcctgctt cttttaattc gtgcaatgta gcaactgatt cattttttgg
    16141 tgtgctttca tccggaaagt ggatataaaa aatgtcgatg taatcagttt gcagacgttt
    16201 tagtgcatct tccaccgatt gtttgagaaa ctcaggagaa ttatcaaatg ttcgcccttt
    16261 atttggtaca tgagcagctt ttgtagcgat gacgacacgt gagcgatcat attctttttc
    16321 cttcagtacc tcgccaatca gttcttcgga acggccgttt ccgtaagcat aagcggtatc
    16381 gatcaaattg atcccgctat tcaaagctgt acgaactact tgttttcctg tctcatcttc
    16441 caaaccgtca aaaagattat gtcctccaac ggcatttgct cctaatccaa gctgttctgc
    16501 gtagacttgt gaatgtccga ttcttatttc tgccatagct gattcctcct ttttgtctag
    16561 tctaaaacaa aataccaaaa agctgaacta aaacgttttt gtgaggggaa atagtcagat
    16621 atctcttgac tttgagactg gtttttagta ggttataagt aatatcgaat gtcaatgaaa
    16681 gagcgagtaa gtttttgaaa ctttacaaga gaggatcatc gaggctgaaa atgatctaaa
    16741 gtttcattag cctaaagaca ctctggagat ggacgagtga aaagccgtcc cgcgtacgag
    16801 cgttattcgt aagaggaaag agaaatcttt caaaattagg tggtaccgtg tatgaatttc
    16861 attcaccctt gttcatgatg aacaagggtg atttttttat ttacatagct aagaaaggga
    16921 gagaaaacta tgagtttcca aagaccaaaa ggaacaaatg atattttgcc aggcgaatca
    16981 gaaaaatggc agtttgtcga agaaacagca cgtttattat tcagagatta tcagtataat
    17041 gaaatgcgta caccgatttt tgaacactat gaagtgatat cccgaagtgt aggggataca
    17101 acggatatcg tttcaaaaga gatgtatgat ttttatgaca aaggggaacg ccatgtgacg
    17161 cttcgaccag aaggtacagc accaatcgtt cgttcttttg ttgagcataa attgttcggc
    17221 ccagagtatg cgaaaccgtt caaaactttt tatatgggac caatgtttcg ctacgaacgc
    17281 cctcaaaaag gacggttacg tcaattccat caaatcggag tagaagcatt tggatcagaa
    17341 aaccctgcaa ctgatgtgga gagcatggtg atggcacttg atttcttcaa acagttgggg
    17401 atcacgcaaa tccggctagt catcaattca ttaggtgata aagaaacgcg tcaagcctat
    17461 cgtcaagcat tgatcgacta tcttttacct tttgaatcag agctgagtga agactctaaa
    17521 cgccgccttc atgaaaatcc attacgcgta ttggacagta aagacaaacg agatcaaaaa
    17581 tttgtggcag aagctccatc tatcttagat tatctaagcg agcctgctca aaaacatttt
    17641 gataccgttg tttccatgct ggatgcattg gctgttcctt atgaaatcga cagtaatatg
    17701 gttcgcggac tagattatta cacacatacg atttttgaaa tcatgagtga cgcaccaaaa
    17761 atgggggcac aagcaacgat ttgcgcaggc ggacgttatg acaatctagt ggaagaacta
    17821 ggtggtcctt caacacctgg cttcggattt gcaatgggta tcgagcgatt actcttgact
    17881 atggaggcag aggaagtagt tatccctgca tttaatgagt tggatgctta cgtggtcgca
    17941 ctcggagacg aaacaaatat cgaagcattg aaagtggtcc aagcgattcg aaactttggt
    18001 ttttcagctg accgcgactt catgaaccgt aaagcaaaag cgcaatttaa gacagcagat
    18061 aaagcgaatg ccaaattagt attggtgatc ggtgaagatg agcttgctag tcaaacgatt
    18121 aatgtcaaag caatggcaag ccgaaaagaa aaaagttttc cattagaaga gatatatgaa
    18181 cattttgatg aagtctatga tgaaatgact ttattcgagt aggaggaaaa aaataaatgg
    18241 cacaaagaac gatctattgc ggattagttt ccgaacaaca attagaacaa acagttacat
    18301 taaaaggatg ggtacaaaaa agacgagacc ttgggggagt aatctttatc gacctacgtg
    18361 atcgtgaagg aatcgttcaa atcgtcttta atcctgaaat caacaaagaa gcatgggaaa
    18421 tcgcggataa atgtcgcagt gaatacgtga tcgaagtgac tggggaagtt agaagacgcg
    18481 gagaaaatgc agtaaatcct aaaatggcca ctggtgaatt cgaagtgatt gcaagtgaaa
    18541 tcgtgatttt gaatcaagca aaaaccccgc catttcaaat tgaagatgat caagcaatca
    18601 gtgacgaaat acgtttgaaa tatcgttatt tggatctgcg tcgtccaaaa atgactaaaa
    18661 acatcaaatt aagaaatgcg acaacaaaag cgattcgtca ttatttagat gataatgact
    18721 ttctcgatat cgaaacacct tacctaggga aatctacacc agaaggcgca agagactacc
    18781 tagttccttc acgtgtccat gctggacatt tctatgcgtt gccgcaatct cctcaattgt
    18841 ttaaacaatt gctgatgaat gctggattcg accgttatta tcaaatcgtt cgttgtttcc
    18901 gcgatgaaga tttacgtgga gaccgtcagc ctgaatttac gcaagtagat attgaaacaa
    18961 cgttcttaac agcagaagaa atccaaactt acacagaggg attgattgcg aaggtaatga
    19021 aagaagttcg cggtatcgaa gtcacattgc catttcctcg tatgacctat gatgaagcaa
    19081 tggctcgcta cggcagcgac aaaccggata cacgttttga tatggaattg atcgatctaa
    19141 gcgatacagt caaagaagta gaattcaaag tcttccaaat ggctcttgaa aacggcggtg
    19201 tcgtgaaagc attgaatgct aaaggcgctg cagatcgcta ctcacgaaaa gacatggatc
    19261 agttaggtca atatgtagga caattcggag ctaaggggct tgcatggctg aaagtagaag
    19321 aagacggatt gaaaggtcca attgctaaat tcatgggaga agcaacagaa gcaatcatta
    19381 aagcaacaga tgcaaaacca ggtgacttgc tgatgtttgg cgcagataaa agtgaaatcg
    19441 tcgctgctgc attaggtgct atccggacac gtttaggaaa agaattagga ctaattgatg
    19501 aatcgaaatt caatttctta tgggtaactg attggccaca atttgaattt tcagaagaag
    19561 aaggtcgata tgtctctgct caccatccat tcacgatgcc aaaagaagaa gatatcccgt
    19621 tattggctga agaccctgca aaagtctatg cagaagctta tgatatcgtc ttgaacggtt
    19681 atgaattagg cggaggttct atccgtattc atactcgtga gcttcaagaa aaagtactag
    19741 aaactctagg ctttacaaaa gaacaaatgg aagaacaatt tggtttcttg ttaacagctt
    19801 tagattacgg attccctcca catggcggta ttgcattagg attggaccgt tttgcaatgt
    19861 tattagccgg agaagataat attcgtgaag tcattgcctt tcctaaaaac ggaaaagcag
    19921 cagatgtgat gagtgatgca ccaagtatgg tttcgccttt acaattgttc gaattgaata
    19981 ttgatgtaac gaatatagaa gaatagccga taaaaaggta aaaaggattt gtcgaatacg
    20041 cttcttttga caaccttgat ttaattgaaa aacactttat aatgaagcca taacatataa
    20101 agcggggctt ttccatgaaa aaaacattcg aaaattgggc ttatcatgat tacacgacca
    20161 agctttcggt atctatcgtt tatgcgttga tcgcatcagt cgcattgaat ttcttttatc
    20221 agccaggaca tatttattca agtggtatta ctggtctagc tcagattttg acgactttgt
    20281 caaaatcact gataggattc gaagtaccgg tatcaacaac tttgtattta ctgaatatac
    20341 ctttgtttgt attggcgtgg ttaaaaatcg ggaagaagtt tacaattttc acttttatca
    20401 cagtcacttt gacctccctt tgtatccata ttttaccgga aacagtccta tctaccgatc
    20461 caatcatctg tgcgttgttt gggggggctg tgatgggact aggtattggg ctagcgttga
    20521 gaaacggcct atcatcgggc ggtctagata tcgtgagtat cacgattcgt aaaaaaacag
    20581 gaaagtcgat tggttctatt tcgatctact tcaatgcatt gatcgttttt gttgccggaa
    20641 ttctatttgg ctggcaatat atgttttaca gtacgttgtc tattttcgtc agtggaaaag
    20701 taactgatgt cgtttatact aaacaaaaga agatgcaggt aatgatcgta acgaaacatc
    20761 cagaaaaagt agtaaatgaa atccaaaact gtatgcgtag agggatcacg atcatcaatg
    20821 aagcagaagg tgcttataag catgataagc agacagtatt gatgacgatc gttacacgat
    20881 acgaactgcc tctacttaga gaagcaatga aagcttctga tccacaagct tttgtaagta
    20941 ttgcagaaaa cgtccagata cttggtcgtt tctatgagga tggcatttag atcgagtttg
    21001 acaaaaattc atgaaatact gctgtagaca gacgttcgtt tgtctgcagt ttttttctac
    21061 catagtagaa tcagcaaaga aaaactgctt ttttatttga aattttctta aagttgaaaa
    21121 aaagcatgtt tattttttta aatttatggg atgatagaaa aaaatagtag agaaaggaca
    21181 cgattctact tgctattttc acttaatacc cgtatattac gaatgaaaaa agtttatgaa
    21241 aagttggaat attgaatgga aaaacaaaaa aggttaaaaa acagccgcta catcactggg
    21301 tttgatgggc tccgttcatt agctgtggtg ggtgttatct tctatcacct tctgccaact
    21361 agtatgaaag gcggctattt gggcgttccg atttttttcg tcgtatctgg ttatttgatc
    21421 acggatctgc tgcggcagga atgggaacaa aatggaaaaa tcaatatttg gcaattttat
    21481 gtcagaagaa tgaagagact ctatccggca cttgttttta tgcttgtgac agcttcggct
    21541 tatatcactt tgtttcagcg agggctgtta aacaatcttc gaggagttgt ggtcagcagc
    21601 ttactgtatg tcaacaactg gtggcaaatc aataacggtt tatcttattt cgatcgattt
    21661 gctaatgagt caccgtttac gcatatctgg tctttagcag tagaaggtca aaactatttg
    21721 gtttggccga tcttgtttgt attgttgatg gtatttgttc gtaaaagaaa atggatcttt
    21781 tatactgtgt taggtacatc cttcctctcc gccatcttga tgatgatctt gtatacacca
    21841 ggaggcgatc caacccgtgt ttattacggt accgacactc gtctgttttc tatatggttg
    21901 ggaagtgcgc ttgcgtttgt ttggccaagt acgcgtttaa agaaaaacat cccgaaacaa
    21961 gcgaaaaata ttctgaatat cgctggagga atctcgcttt tggcactgat tctttgcttc
    22021 ttctttttag atgatcatta caatttcatc tattacggag gaatgctcct agtgagtctg
    22081 ttatgcgtag tgctagttgc agtgacagca caccccggag caagtttgaa tcgatggctg
    22141 acaaatccag tatttacttg gattggcaaa cgaagctatg gcatttatct gtatcagttt
    22201 ccagttatga ttttttatga agcaaaggtg actgatattg ccgatcatgt tttgttgcat
    22261 actttagtgg aaatcacatt gattctaatc atcagtgaac tttcttatcg atatgtggag
    22321 cgtccgctag cacgttttga ttatggtcgt acattccaag tgatgaaagg atggttcagc
    22381 aaaccgatca tcagtaagag caaaccatgg gttgttccag gcacattact tgtagcaatt
    22441 gcactcgttg gtttcgttac ggcaccgaaa aacgctgtaa cagccgatca aaaacaactg
    22501 caggcaaaaa tcgctgaaag taaaaaatta gcagaagaaa ccagaaacaa ttcagaaaac
    22561 accactagtt cggttgacca agcgatcctt gataagtacg agttgtccaa ggcacaaggg
    22621 aataaggcac aaaaattaga actaacggca tttggtgatt cagttatgtt ggatgctgca
    22681 acggatctac aagaacttta tccaaaagta gtggtagatg gagatgtcgg acgacaatta
    22741 tatgccagtg taccgtacat tgaaaaatta aaggaacaag atttgttgaa agacactgtt
    22801 ctgattggac taggaacgaa tggctcattc tcggaagctc agtttgatag tgtgatggat
    22861 gcaatcggtg atcgaaaagt atactgggtc aatgtccgag taccgacgaa gcgctggcaa
    22921 aatgacgtga atgcgatgct tgaacagatg acgaaaaaat acgacaatat gacgctgatt
    22981 gattggtaca gctacagtaa tgaccatagt gactggtttt atgatgatca agtccatcca
    23041 aatcctgaag ggatgacaca ttacattcac ttagtcagcg aagccttgct aggaggaagt
    23101 tcaagtacga acagcagtga gacaaacgtg acagaatgat agctacaaaa aacttcaggc
    23161 aaaaacattt gtttttgcct gaagtttttt tatatttacc gtgtttttaa cattcatata
    23221 gattcaggaa gcaaaacggt tgagtgtgtc tgtaaataac gttctttttg ttttcgggtg
    23281 agttttttga atgcagcttc tgcttttgtt gcttcgcttc ttgaagaaaa tacttctgca
    23341 tggatcatct taacaggtct tcttttttga agacgagtat atttagcacc agtaccattg
    23401 ttatgttcct ctaatcgtct agaaagatcg gttgtatatc caccataaaa tgtctgatct
    23461 ttacagaata atacgtaaaa ataatgttca tttgccatag agcatctcac gaatctctgc
    23521 agtgtattct ccatccatat tgtaagtgat caatggtgga gcgactttga atccgtctaa
    23581 tttgccttct ttgattcctt caactaacaa aatatttgcc tcttttccag gttttgggta
    23641 aacaaattgg atccttttag gtgcaattcg atagtcttcc atgacctgaa tgatttctaa
    23701 gaaccgatca ggacgatgga ccatagtaaa gcgtccattt gtttttagta gcttacttga
    23761 aatatcgatc acttcttcta acgtcgtatg aatctcatga cgggcgattg ccaggtgttt
    23821 attagggttc gttttatttg ttggcagccc tttgaaataa ggtggattac agacgactaa
    23881 gtcacaagaa ttatgtcgaa cgacagataa gctgtctttc agatcgatgg tgtggacagt
    23941 catttgttca tttaaatggt taagcgcgat actacgtctt gccatgtctg ctagacgagg
    24001 ttgaagttcg atagcgtcga tagccgcctg ggttcgttta cttaaaaaaa gtccaactgc
    24061 gccatttcct gcacaaaggt caacaatctt ccctttttta ggtacacggg aaaagtgagc
    24121 aagcagcaca gcatctaaag aaaaagaaaa cactttggaa ctttggataa tcttgatatc
    24181 ttctgcatat aattggtcga ttcgttcgtc ttcgtgaagc atggattccc tccaaaatga
    24241 aaaaaatagt cgtctgtatt atactatttt tattaaaaag aaccaaggtg gcttgtcaaa
    24301 cctctatttt ttatgcatac tatgaccata agaagggaaa ggaagagact atgttttatc
    24361 gatttatgcg cggggtagtt cgagtactat tagccttagt taatggaaat gcccgctatg
    24421 agaataaaga agttttaccg caagatgaga attacatact agttgctccc catcgtactt
    24481 ggtgggaccc attatatctg gcggtagctg cagcacccaa ggaattcagc tttatggcaa
    24541 aagaagaact gttcaaaaat ccagttctgc gatttatatt aactcgatcc aatgcttttc
    24601 cagttaaaag agaaaatcca ggaccaagcg tcattaaaac acctgtaaag attttgaaat
    24661 cgacaaattt gagtttgatc atgtttccca gtgggacaag acatgctaca gaacttaaag
    24721 gcggaatggc attgatttcc agaatgtcta aagcgaaaat cgtgccagct gtatatcaag
    24781 gtccgttgac acttggggat ctgttcaagc gtcagagagt gacagtgcgt ttcggcgaac
    24841 caatcgactt atcagatatc aagaaaacgg ataaagaagg tatggcggag atcgaacgac
    24901 gaacacaagc agcttttgac aaattggaca aagaagtgaa ccctgatttc aaatatgaag
    24961 tcaaaaaaaa ataaaatgat aagtttatgc gtgggcggtc ttttgaatct actgtcctgt
    25021 tcagttcaga ctttttcaat gaaaacgagg gagagacgtc aagtctgccc tctttttgcg
    25081 ttccaatatg taaaatagaa gacaggtgtc atgtaatgga tcaaagtgtt ataataatta
    25141 gaaggacatt ttttgaggtg attgaatgcg ttttttacat acagccgatt ggcatatcgg
    25201 aaaaaaacta caaggttatg acttgattga agaccagcgt catgtactat cttctatact
    25261 agatatcgcg aaagaagaaa aagtagaggc aatcgtgatt gccggagact tatatgatag
    25321 gagtgtccca gctgttgatg ccgtagaatt atacaatgaa ttgatggtcg attggaattt
    25381 aaaggaaaag atcccgattt ttgctatatc aggaaatcac gatagcagta cgagactttc
    25441 agcgggtacg ccatggttca gttattcgaa ctattttttg cgaacacagc tagcagaagc
    25501 atttgaacca gtcgagttta aaaacgtaca atttttcttt cttccttact ttgaaccaat
    25561 tgcagcgaga ttatattttg aagatgacaa tatccgaacg atcaagcaag cggtagccaa
    25621 ggtgatagat aaaatgcagg agctatttga ttcagaaaaa aaacaagtat tgattagcca
    25681 tttttttgta atgggaagtg taaaatctga ttcggaaacg aaaatcgaag taggcgggtt
    25741 ggattcgatc cctggtgaac tacttacacc gtttgattat gtagcactag gacatttgca
    25801 taatcaagct gctttgagac aggataatgc tcgctatagc ggttccccat tgaaattctc
    25861 tttatctgag atcaaccaaa aaaaaggcgt atggatcgtt gacctgacaa caggcctgga
    25921 gttgaatttt agggagatcg aacccctgca tgatattgaa gaaataacag cgagtttcaa
    25981 agaattgctg gatcctgcct tttatcagtc cgtcaatcgg gagaattaca tctatatcta
    26041 tctcactgat agaacgatca tcccgaatat gatgaaccag cttcggcaaa tatatcctcg
    26101 tattttgggg attgaacggg caaacggtcg agcagaacaa acaaagcgca aacgaaaagt
    26161 actagaaatg aaagatcctc aaaaattagc agaagcattt tttagtgaaa tgacggaaga
    26221 gcagctaact gcaaaccaag aaaaatggct cggagaaacg ttgcaagaaa tcatggaaat
    26281 ggaatgatca agaatgaaac cgaaaaaatt acaacttaaa aattttggtc cttttatcaa
    26341 tgagaccgta gatttttcac gtttgacaga agcaccgctt tttttgatca gcgggaagac
    26401 aggagctggc aaaacgacga tttttgatgg gatcaccttc gcactttttg gtgaaacttc
    26461 cggaagactt cgctctggaa aagagatgcg ttctttgttt gctacaccag acgaagaaac
    26521 aagcgtgact ttttcttttg aacatcagca gatgagctat gaaatcgaac gaaaaccaga
    26581 acaagtttta gcaaaacgaa aaggaaacgg aacaaaaaaa caggcagcca aagtaacgtt
    26641 gaccattttt gatgggaaag gaaaagaaat aaaacaaatc acaaaacgta cagaagcaga
    26701 tcagttgatc agagaactga tgaatctcga tgcgaaacag ttttctcaaa tcgttttact
    26761 accccaagga gaattccgta attttttggt ttcatcaagc agtgataaag agactgtttt
    26821 acgtcattta ttcggtactc aatttttcca acagttcaat gagcgattaa aagaaaaagc
    26881 aaaacaacag caacaaaaat tggatcattt agagcaagag ctgcatcttc tgcaaaaaag
    26941 gtttgtttta gtcaaagaag aggaaccagc agcagggttt gaagccgttt tagcccaatg
    27001 gacaaaccat caggcaatac tcgaaaaaca aatcacggaa gaaaaagaag ctctaacgga
    27061 acttcaggaa aaacaaaagc aactagaaaa ttcctactat cgttttgaaa gcttgaaaaa
    27121 aagttatgaa gaaaaaacga tattaataaa caagcaaaag gaattagctg ctcaaaaaga
    27181 cgaaatagag gaaaagaaac gatggattgg gtattatgaa tttacgcaac gattagtcga
    27241 accaatccag cgcctacaag aaataaaagt tgaaaaaata gaactgtcac aaaaaagtag
    27301 tgagcaaaca aagaaactag cgatgactac gcagaattat gaagagtggc aaaaaaatga
    27361 agcaaaccgc gtacgccgac aaacaaaaat caaagaagct gctcaaaaac tgcaaaacaa
    27421 acagatgatg gtgccaattg tcgaatcctt acgtagtaaa aaacaagacg cagaacaatt
    27481 aacaatttca ttggataaag aagaattgaa tgccgagaag ctgtatcaaa atctcgctat
    27541 ctatcaggaa agaactcgaa ttcttcaaga aaatttactg gatcaggaaa aactgcaaga
    27601 agatcgtctt gcttttggac agttaaaata tctagatgaa aagcagcaag aaatagaaaa
    27661 acaaaaagca aaagacactg ggtcgctcgc acagataaga gaagaactca aaaaagcaac
    27721 tgaagaaata acaaaaaata gtcagctatt gaaagaacag acagaacaag cagaacagct
    27781 gaaaagccaa tgggcaaagc agcaaattgc tcgtcttcag ctttttttgc gtccagggga
    27841 gccatgtctt gtttgtggat caacggaaca ccccatacaa caattaaaac acgaacagcc
    27901 aaataaggaa gatatcttaa aagtagaaca acaactggca gaagctgagt ctgctgtaga
    27961 aagaactcag caaatcattg ccaaaagtga agcacatgaa aataatcttc aacaacaatt
    28021 atcagattta gaacaaaaaa tcatggcaac gaatatacaa ttggctgaac aagaagaaca
    28081 cttgcaacag gctttgaaaa aggcatttcc tgagataaca gaacaaaaaa caaaaacaac
    28141 tcttgtagta atcgaacaat ctttagatag acgaaaaagc aagatggcca atgcaagaat
    28201 agagctggaa gaaataagac aacaagcaga aaagctcgaa caacaattag ctgtctacaa
    28261 acagcagcag acagaaaaaa ataatcagct ccagcaactg caaggagaaa tcaaaagttt
    28321 agaacaacaa ttgcaagata cggatagtaa ctgtttagaa gaggagatac gacgacttga
    28381 aaaacagtta gaagaagact cgaatcaact agctgttgag cagaaagcag gagtgcaatt
    28441 gttgcaagat cttcatacgc ttcggacaca gcttgatctt caacatgaac aaatagaaaa
    28501 aatagatgaa aaaatagaaa aggaacaaga aagaatagct aaaaagttag aaagccagtc
    28561 gtatttttca acaattgaag aaatctgtgc atttgcacaa cagcaagcac aatataaaga
    28621 aaaccaacgt acagttactt cttatcatga agagcaatta gtgacagctg accggctgcg
    28681 tcaattagaa aatatccagc taactgagac attcccagat acagaagaga tcagccaaca
    28741 gttagaagaa ataagacgta tgatcgaaat aaagcaagaa aagatatacg gtttacaaga
    28801 acaaaaaatt agtaatcaaa aaatctacga tgaatgtctt tcgatttatc acacaagcca
    28861 gacgaaatta gatgagctta cgcaattaca gcagctttcg caaacattca acggagaaaa
    28921 tcctaagaag actagtttgg aacggtatgt gcttcaggtt tatcttcaag aagtgttaca
    28981 agtagcaaat gatcatctcc aacgtttaac gaaaaatcgt tatcaatttg agctagcgga
    29041 taccattggc agttatcgag ggaaaaccgg attagaaatc aatatctatg atgatcaggc
    29101 aggtatgtct cgaagtgcgc ataccctgtc tggcggtgag agttttattg cagcactttc
    29161 tttagcatta gctcttgccg atgtgatcca gacacaagct ggtggtgtag caatcgatgc
    29221 attgtttatt gatgaaggat ttgggtcatt agatgaagaa gctttggaaa tggcaatgga
    29281 agctttagaa acgatcgaaa gtgaaggaag aatgatcggc atcattagtc atgtaagaga
    29341 attgaaagaa cgagtattac aacaaataag aattgataca gaaggtagcg gacaaagcaa
    29401 aatacgctat caacttggat aaaaagggag attggtaact atgaaatgga gtattccaga
    29461 aaaagtcatt gcacgtggac gtcaatactt gcaagaaggc cgagtgttat cggttgtccc
    29521 tgaccaagaa gaaaacgtgt ggcatgcaga agtcttgggc agtgaattgt atatggttga
    29581 tttggatggt acagcaaaag agatagatta ctgccaatgt ccctattggg aggaacataa
    29641 atattgtaag catacagttg ctgtggaact ttatttacgg tcgaaaaatc tatctcgtat
    29701 catgaaaaaa gaccagccaa tgctagaacc ggtaaaagca acaagtgagg gagaaatcat
    29761 tgcaaaaggt ttttctcgtc taaaaacaca aaacaatcct caagcggtgc aacctttgat
    29821 tattgaatac caagtagaaa cgattgagac taatcagtat cacccagagc tttctatttt
    29881 ggcactttac ttgcgtatcg gttcattggc atcaccgaaa aaaacatata tcgtaaaaaa
    29941 catctatgat tttttacaag cttacacaga aaaagaaact tacactgtta ataaacagta
    30001 ccagtttcgt ctagataaac gggcgtttca aaaagaagat caaaaaatac tcacgcatct
    30061 tgcaggaagt gctcagacgc atcaattatt aggtacgaac ggcttacaag tcaaaggaaa
    30121 actagataaa agatatctgc ttctcccagt agaacaagcc cgcttcctgt tagagcagat
    30181 gaatcagact acacgtttgc atctgcagat aaatgaacaa aacgatcaag gaatacaatt
    30241 ttccgatcaa aacaaacctt tatcattcaa agtggaaaaa tttggagaag attatttgct
    30301 gcaagcattg aatgattttg attttttctt tatgcattat cagtggggag ttatccaagg
    30361 gacattttat tctctgacga aatatcaaca aacggtttat caaacttgga aacaattgat
    30421 tcgccgattg gagaaaccag aagtagtgtt caagaaaaaa gaattaccgt ctttgtttaa
    30481 agagattatc cctctgctgt cagagatcgg cgacgtagct attgcccctg cagtagaaga
    30541 ggaagtagca gatatccccg tcgaatttat tttctttttc cgaaaagcaa aaggaaaaat
    30601 ccaagctcga gtcgatttcg tctatggtga agtcatctat tcgactgacc cgaagcatga
    30661 ggtctcgaca gaacaggctt ttcaggtgtt acgtgacaca gtagaagaga aacgagtact
    30721 tgatctattc agaatgtatc gctaccagaa aaatgaaact ggttatgaac gtccattgcc
    30781 aagcggggaa gaactatatg cctttttccg cacagaacta tctgtcttcc gtcagtttgg
    30841 tgaagtcaga cttggaagaa aattgcgaga gttgtattta gatgcgcata aacatcaacc
    30901 gaaaatcgaa gtaaccgaga ccggttcatg gctcgatatt cgattcgatg tgacagggat
    30961 agaggagcaa gaaatcgatc atgtcctgca aagcttgctg agaaatgatg ctttttatac
    31021 attagaaaat ggtcaggttc tttcttttga ttcagaagaa tttcaacaaa caagccaagt
    31081 gttgcaacaa ttacgggaat cgatacgtac agaagaagga acgatccatg tacctaaaaa
    31141 tcaaggattg atcattcaaa atcaattgga aaaaagcaat gctactttta gtgaatcgtt
    31201 ccaaacgatg gtacaagatc tcattcatcc agaaagatac caggcacagc ttcccaaagg
    31261 attgaatgca acgatgagag attatcaaaa gcaaggtttt cgatggttga agatgttagg
    31321 tcattatcag tttggcggta ttttagccga tgaaatggga cttgggaaaa cactacagac
    31381 aattgccttt ttactttcgg aaaaagaaga acggaaaagc ttctctgctt tgatcgtagc
    31441 gcctgcgagc ttaatttaca actggcaggc agaggtgaga aaattcgccc cttctttaag
    31501 tatccaagta atcaatggga ataaaaaaga acgtgaagaa ttactggcta aagacacaga
    31561 tatccgagtg acttcttatg ccagcttaag acaggatcta gctaattatc agtcgcaaaa
    31621 aattgattat ttgattcttg atgaggcaca aatggtcaaa aatagcagta cgaaaactgc
    31681 acaagcattg agagaattag ctgtacctca gcggtttgca ctgagtggga caccaatcga
    31741 gaataattta gaagaattgt ggtcattgtt tgcaacgatt atgccaggat ttttcccgac
    31801 taaaacaaaa ttcagggaac ttgccgcaga agaaattgcg caaatgattc gaccgtttat
    31861 tttacggcga gataaacaaa ctgtgttgaa agacttgccg gaaaaaacag agatgaatct
    31921 atattctgca cttacagaag aacaaaaaac tgtttattta gcttacttgc gtcaaatgcg
    31981 tgaagaaatc acatcaatgg attctgaagc attcaagaaa aaccggatcg ggatacttgc
    32041 tggtctgaca agactgcgac aaatttgctg cgatcctcgt ctgtttattg aagattacca
    32101 aggcggttcc ggcaagctcg aacaagtaaa agatctactg gtagctgcca aagagaacaa
    32161 acgaagagtt ttgctgtttt ctcaatttac agggatgctg actatcttgc aagaagagtt
    32221 agccgagcta gggatctcga cattttattt aagaggcagt acgaaaccac aagatcggtt
    32281 aagtatggta gacgcattta atgctggcga aaaagatgtt tttctgattt ccttaaaggc
    32341 tgggggaact ggattgaatc tcacgggagc agatacggtc atcttgtatg acctttggtg
    32401 gaatcctgcc attgaagaac aagctgctgg acgtgcacat cggatcggac aaaaaaatgt
    32461 ggtagaagtg tggcgtatga tctctgaagg aacgattgag gaacgcatgg attcactcca
    32521 gcaagaaaaa cgcgaactgt tccaaaaagt gatccaaggt aatgaagaac agttgacgaa
    32581 actgactgaa gaagacatcc gcatgatttt aagtgtagga gaaatcgagg agtaagagac
    32641 tcgaaaaagc gttcagtaat gggtaagaaa gcctttctaa agaagcgatt gtttatttga
    32701 tttaaaaaat agcaaggagg ctgggattcc attttatccc agcttccttg ctatcatttt
    32761 tttatttcta tttttaaagg ttatacaaag attgtcttca ttgtctctaa cagctgatca
    32821 aaagaagaaa tctcgatatc gcaaattgga gtctgttcaa ttttgcgttc tcgatggttg
    32881 aaccaaagcg cttgccagtt agcacttttt gcacctagaa catcattgtc gtaattatcg
    32941 ccaacgtata aggtctcttc tggaagcata tgaaattctt tctcagccag ctgaaaaatc
    33001 tctttttctg gtttttggaa acctgttgct tgagaaatga gcatattgcg ggaaggaatc
    33061 caattgttca attgaagttg cttcagtttt tttgtctgat ggtcagtcgg cccatttgtg
    33121 ataattccta atgggacatt cttttccttc aagaaatcga gggttttttt gacctcctca
    33181 tgcaagcaga tattatcaag ttcttcctcg taaatttttt gaaataagag gccatcttct
    33241 tccgtaatat gagggtagtc taaatctttt agtgattggc tgatccgatg tgctcgcata
    33301 tattctagtg tccaatctcc tgccattact tttgggaagt tttcatcact atgatggcgg
    33361 aaccggatat atagtggatg catatctgca tctgaaacaa gcggaacgac tcttttgacc
    33421 gcgtttcgaa atggctgctg ctgatcatac atcgtatcat caacatcaaa cacaactgat
    33481 ttaatcaaaa ttttacacgc ctttcggttt tcattgattt tttcacaaat ccttgactat
    33541 tgtaacagaa aaaattgtct aaaaaagctg ataaataaag gtttttctta ctatatttct
    33601 atttccttga cagaataagg tttttatatt tcagtttttt tcactaaaaa tcgtgaaaca
    33661 cgtcaataag tttgatgaac aaattaatca ttatggtaga agtcgattga tttattttaa
    33721 tgcaatctct gtggaatttt cgaaagattt atttttttaa tacagtctca atataactat
    33781 tcaaacataa aataacgtta tttgatttat ttgtttatag taaattttat ttagtaatca
    33841 ttgctttaaa atactacgct tatataacag agaaaataag cgttataata aagaacagta
    33901 ttatttaaaa tgacagaaaa cattactatt aaagaaataa aaaacataaa aaatcttaag
    33961 aaatcggttt catttagctt ttgagataaa ataatagtac agttttatac gctgtttcat
    34021 atgtttagaa aatttgaatt agatttgaaa actctaattt ttaaatgata aaaaaagcga
    34081 gaatgctggt tttactgtat attaacctta gataactttc atttttttaa aaaaattatg
    34141 aaaaaaaggt agctatttaa aagttgatta tgtataatag aaacagatgg gagattatta
    34201 tgatcttctg ttacaaaaaa gtgattctta tcacgaaaag gagtggtgca cgttgttaac
    34261 attatatact tccccaagtt gtacttcctg cagaaaggct cgtgcgtggt tgcaagaaca
    34321 tcagattcca tttgttgaac gtaatatttt ttcagaacca ttgaacagtt ctgaattaaa
    34381 agcgatttta caaatgacag aagacggaac agaagaaatc atctcgacac gttccaaagt
    34441 attccaaaaa ctgaacatgg atttagatga tctgccgtta caagaattat tggaacttgt
    34501 gcaaaacaat cctggattgc ttcgccgtcc aatcatgatt gatgataaac gtctgcaagt
    34561 tggttttaat gaagatgaaa ttcgtcggtt cctaccgcgc gatgtacgac aattagaatt
    34621 acgtcaagca caattgatgg ctggattata gaaggataat acgaaacttg gaaaaga
//
LOCUS       DAFBQH010000024        33709 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.024, whole genome shotgun sequence.
ACCESSION   DAFBQH010000024 DAFBQH010000000
VERSION     DAFBQH010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.024"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            1..813
                     /locus_tag="LK694_001216"
     CDS             1..813
                     /locus_tag="LK694_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBK5052098.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            810..1610
                     /locus_tag="LK694_001217"
     CDS             810..1610
                     /locus_tag="LK694_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBK5052099.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1771..3039
                     /gene="rseP"
                     /locus_tag="LK694_001218"
     CDS             1771..3039
                     /gene="rseP"
                     /locus_tag="LK694_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBK5052100.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3107..4816
                     /locus_tag="LK694_001219"
     CDS             3107..4816
                     /locus_tag="LK694_001219"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBK5052101.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANNLPILLN
                     QGE"
     gene            5028..9380
                     /locus_tag="LK694_001220"
     CDS             5028..9380
                     /locus_tag="LK694_001220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBK5052102.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9524..9997
                     /gene="rimP"
                     /locus_tag="LK694_001221"
     CDS             9524..9997
                     /gene="rimP"
                     /locus_tag="LK694_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBK5052103.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10021..11196
                     /gene="nusA"
                     /locus_tag="LK694_001222"
     CDS             10021..11196
                     /gene="nusA"
                     /locus_tag="LK694_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBK5052104.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11218..11511
                     /locus_tag="LK694_001223"
     CDS             11218..11511
                     /locus_tag="LK694_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBK5052105.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11508..11819
                     /locus_tag="LK694_001224"
     CDS             11508..11819
                     /locus_tag="LK694_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBK5052106.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11832..14138
                     /gene="infB"
                     /locus_tag="LK694_001225"
     CDS             11832..14138
                     /gene="infB"
                     /locus_tag="LK694_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBK5052107.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14165..14512
                     /gene="rbfA"
                     /locus_tag="LK694_001226"
     CDS             14165..14512
                     /gene="rbfA"
                     /locus_tag="LK694_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBK5052108.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14620..14841)
                     /locus_tag="LK694_001227"
     CDS             complement(14620..14841)
                     /locus_tag="LK694_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5052109.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15289..17651
                     /locus_tag="LK694_001228"
                     /pseudo
     CDS             15289..17651
                     /locus_tag="LK694_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18019..18942
                     /gene="truB"
                     /locus_tag="LK694_001229"
     CDS             18019..18942
                     /gene="truB"
                     /locus_tag="LK694_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBK5052110.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18946..19887
                     /gene="ribF"
                     /locus_tag="LK694_001230"
     CDS             18946..19887
                     /gene="ribF"
                     /locus_tag="LK694_001230"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBK5052111.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20239..21024
                     /locus_tag="LK694_001231"
     CDS             20239..21024
                     /locus_tag="LK694_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK5052112.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21037..21831
                     /locus_tag="LK694_001232"
     CDS             21037..21831
                     /locus_tag="LK694_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBK5052113.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKN"
     gene            complement(22011..22463)
                     /locus_tag="LK694_001233"
     CDS             complement(22011..22463)
                     /locus_tag="LK694_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5052114.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(22928..23296)
                     /locus_tag="LK694_001234"
     CDS             complement(22928..23296)
                     /locus_tag="LK694_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HBK5052115.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23446..23928
                     /locus_tag="LK694_001235"
     CDS             23446..23928
                     /locus_tag="LK694_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBK5052116.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            23965..24975
                     /locus_tag="LK694_001236"
     CDS             23965..24975
                     /locus_tag="LK694_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBK5052117.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25090..25803)
                     /locus_tag="LK694_001237"
     CDS             complement(25090..25803)
                     /locus_tag="LK694_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052118.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(25993..26583)
                     /locus_tag="LK694_001238"
     CDS             complement(25993..26583)
                     /locus_tag="LK694_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBK5052119.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            27067..27294
                     /locus_tag="LK694_001239"
     CDS             27067..27294
                     /locus_tag="LK694_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBK5052120.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27310..28476
                     /locus_tag="LK694_001240"
     CDS             27310..28476
                     /locus_tag="LK694_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBK5052121.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28710..29753
                     /gene="hrcA"
                     /locus_tag="LK694_001241"
     CDS             28710..29753
                     /gene="hrcA"
                     /locus_tag="LK694_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBK5052122.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            29847..30410
                     /gene="grpE"
                     /locus_tag="LK694_001242"
     CDS             29847..30410
                     /gene="grpE"
                     /locus_tag="LK694_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBK5052123.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30461..32290
                     /gene="dnaK"
                     /locus_tag="LK694_001243"
     CDS             30461..32290
                     /gene="dnaK"
                     /locus_tag="LK694_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBK5052124.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32441..33607
                     /gene="dnaJ"
                     /locus_tag="LK694_001244"
     CDS             32441..33607
                     /gene="dnaJ"
                     /locus_tag="LK694_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBK5052125.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT        11457 a         5812 c         7275 g         9165 t
ORIGIN      
        1 atgttacgtt tttttccgca aaaaaacaaa tatattcaag aaaaaagcga atttcaattt
       61 aataccgatg gaccaattcc tcagcacatt gccatcatca tggacggcaa tggtcgttgg
      121 gcgcaaaacc gtcgtttgcc acgagttgct ggccataaag aaggcatgga aacagtaaag
      181 aaagtcacaa aaaaagcatc aaggcttgga gtgaaagtgt tgacgcttta tgccttttcg
      241 actgaaaact ggaagcgacc aaaagatgaa gtaagttttc tcatgcaact acctgtcgac
      301 ttctttgata cgttcgtacc agaattgatt aaagaaaatg tcaaagttca tgtcatgggt
      361 tatgaaaacg tattgccaga acatacgcaa gatgctgtaa gacgtgcaat tgagcaaaca
      421 aaaaacaata ctggaatggt attgaatttt gctttgaatt atggaagtag agcagagatc
      481 gtgactgctg tccaagaaat tgccgaagaa gtagcaaaag gagaaataca cgcagaagaa
      541 atcgatgatg aattgattgc taagcattta atgacaggtt tcttgcctaa agagttacaa
      601 gatcctgagc tgatgatccg tacaagtgga gaagagcgaa ttagtaattt tctgttatgg
      661 cagatcgcat atagtgagct gtattttaca aaagcgcttt ggcctgattt tgatggtgcg
      721 catcttgaag aagcaatcgc ctcttatcaa aatcgagacc gtcgattcgg cggtgtaaaa
      781 aaaacagaag atgagaaagg ggatcagtca tgaaacaacg tgtgatcaca gcagctgtgg
      841 cgctagtctt gtttattccg attatatgga tcggaggcat agcggttgag cttacagcag
      901 caatcttagc ggtagtagga gtttacgagc tgttccggat gaaaggattg acactcttaa
      961 gttttgaagg agttttatcc gccgttggtg cagtgttact tgttttgcca aaagaaagat
     1021 ggtttttctt tcttccagag aatgtaagta catttatgtt attttatctt atagttatga
     1081 ttcttttagg aacatcagtt atttctaaaa atacgtatac gattgatgaa gcaggttttc
     1141 ctgtgcttgt cagcttgtat gtgggaatag gtttccagaa ttttgtcaat gcacgaacag
     1201 aaagcttggc tgttttgata tttggtctat ttattgtttg ggcaactgat atcggagcat
     1261 atatggttgg tcgacgttat ggtcagcata agttaagtcc tgatatttcg cccaacaaaa
     1321 cgattgaagg tgcgctcggt ggtatcggta gtgctgttgt agtcgcattt ctttatttat
     1381 tgctataccc agcaaaagaa ctattcaatc atggtatgtt tgtcatgctt ctgatgacga
     1441 ttattttttc agcagtcgga caatttggtg atttggtcga atctgcaatc aaacgtcatt
     1501 atgatgtgaa agactcggga aatatcttac caggccatgg tggaatctta gaccgtttcg
     1561 acagtttgct gtttgtattt cctatcatgc atttatttgg cattatttag taagtatacc
     1621 tgcgtttctt tgtgaatttc ataaaagacg caggttttta tttttcatta tattgatact
     1681 attggtgtct ttggtatact atggtaaaat tatagtgttt tatcgttgtt ttataaaaaa
     1741 cacaaaaaac aagtaaagga gaaaaaagct atgaaaacga ttctgacatt tatcatcgtt
     1801 tttggtatat tagtgattgt tcatgagttt ggtcatttct tctttgcgaa acgatcagga
     1861 atcctcgtcc gcgaatttgc tatcggtatg ggtccgaaaa tttatgggca tcaagcaaaa
     1921 gacgggacaa catatacgct tcgcctatta cctattggcg gttatgttcg aatggcaggc
     1981 aatggggatg atgaaacaga aatggcacca ggaatgcctc tttctctttt actaaattct
     2041 gacggcatcg tagagaagat aaatttaagt aagaaaatcc aattgacaaa tgctatcccg
     2101 atggaattga gccgttatga tttagaagat gagctgacta tcactggtta tgtgaatgga
     2161 gacgaaacag aagtagtaac ctaccctgtt gatcacgatg cgacgattat tgaaaacgac
     2221 ggaacagaaa tcagaattgc gccaaaagac gtacagttcc aatcggctaa attgtggcag
     2281 cgtatgctga ccaactttgc tggacccatg aataacttta ttttggcaat cgtgttattc
     2341 atcattctgg catttatgca aggtggtgtt caagtgacga acactaatcg cgtaggagag
     2401 atcatgccta atggagctgc ggcagaagca ggcttaaaag aaaatgatga ggtagtcagt
     2461 gtggacggaa aagaaatcca ttcatggaat gatctgacaa cagtcattac gaaaaatcca
     2521 ggtaaaacat tagactttaa gatcgaacga gaaggtcagg tgcaatctgt cgacgtgaca
     2581 ccgaaatctg tagagtcaaa tggcgaaaaa gttggccaac ttggtataaa agccccaatg
     2641 aatacaggtt ttatggataa gatcattgga gggacgcgtc aagctttcag cggttcattg
     2701 gaaattttca aagccttagg atcactattt acaggtttca gtttagataa attaggcgga
     2761 cctgtcatga tgtaccaatt gtcttcagaa gcagcaaatc aaggtgtaac aactgttatt
     2821 agtttgatgg cgcttctttc aatgaatctc ggaatcgtca atctgcttcc gattcctgcc
     2881 ttagatggcg ggaaattagt cttgaatatt tttgaaggga ttcgcgggaa accattaagt
     2941 caagaaaaag aaggaatatt gacattagct ggctttggat ttttaatgtt gttgatggtg
     3001 ctagttacat ggaacgatat tcaacgattc tttttctaga aaaagttgac aaatagggta
     3061 taataacaaa cgataactat tttgaggaaa taaaggagta tatagaatga gacaatcaaa
     3121 aatgttgatt ccaacattac gcgaagttcc aaatgatgca gaagtattaa gccatcaaat
     3181 cttgctaaga gctggatata tccgccaagt atcagcgggt atttattcat atctgccatt
     3241 agcaaatcgt gtattggaaa aactgaaacg cattatgcgg gaagaatttg aaaaaatcgg
     3301 agcggtagaa atgctgatgc ctgccatctt gccagcagaa ctttgggaag aatccgggcg
     3361 ttacgaaaca tatggaccaa acttgtatcg tttcaaagac cgcaatgatc gaaaaatgat
     3421 ccttggacca actcacgaag aaacttttac tgatttgatg cgtaatgaaa tcaattcata
     3481 taagaaatta cctttaaatc tttatcaaat ccaacctaaa tatcgtgacg aaaaacgacc
     3541 tcgcttcggt ttgcttcgcg gaagagagtt tatcatgaaa gatgcatatt ctttccatgc
     3601 aacggaagaa agtttggacg agacatataa agattacgaa aaagcctata ccgaagtatt
     3661 caagcgttgt ggcttagaat ttcgctcgat catcggagac ggcggcgcaa tgggcgggaa
     3721 agactcaaag gaattcatgg cgatctcaga aatcggggag gatacgatct gttattcgac
     3781 tgaaagtgat tatgctgcaa acttggagat ggctacaagc tactatgtat caaaaaaatc
     3841 gcatgaaaca cagcttgaat tggagaaagt agctacacct aacgtaaaat caattgacga
     3901 agtagcagat ttcttttctg ttgaacctca aaaaatcatc aagtctgtat tgttcattgc
     3961 ggatgaacaa cctgtccttg ttttggtacg tggagaccat gaagtcaatg acgtaaaact
     4021 gaaaaacttt ttatctgctg actttttaga agaagcaaca gaagaagaag cacaaaaata
     4081 tctaggtgct gagtttggtt ctgtcggacc agtcggtgtt tcagatgacg taaaagtata
     4141 tgcagaccgt tatgtccaag atttagcgaa tgccattact ggagcgaacg aaacaggatt
     4201 ccactataaa aatgtgaatc cagaacgcga tttcactgtg ctttcttatg aagatcttcg
     4261 ttttgtacaa gaaggagatc cttcaccaga caacaacggt gttcttgcct ttactcgagg
     4321 aatcgaaatc ggacatatat tcaaaatcgg cactcgttac agtgaatcta tgggagcaac
     4381 tgtactagac gaaaatggac gagaaaaatt tgtgattatg ggctgttatg gtatcggtgt
     4441 aagtcgttta ttatcagcaa tcgtagaaca acattcagat gaacaaggaa tcaattggcc
     4501 aaaaggaatt gcgccatttg atctgcatat cgtccaaatg aatctaaaag acgaatacca
     4561 aacaagcctg acgaatgaac tagaagaagc gatgacaaaa gctggctatg aagtactggt
     4621 tgatgacaga aatgaacgcg caggagtgaa attcgcggat tctgatttga ttggctgtcc
     4681 aatcagaatc actgtcggta aaaaagcagt ggatggaatc gtagaagtga aaatcaagaa
     4741 aactggcgaa atggttgaag tacgtaaaga agaattggcg aataatttac caatcctctt
     4801 gaaccaaggc gaataataag cgttaaaaca cagaaaataa aaatagctga atcagctgtt
     4861 tttgacaatc tgatgattcg cttttaaaat tggaactgcg atttgaatcg cagttccaat
     4921 tttttaatgt aaaactcaga aaaaacgagt atactaaaag gtactgaaaa agaagaggat
     4981 aagatgcgat caattgcatt gtttctcatc agaaagggcg tatatctttg tcagaaaaac
     5041 gagatttatt tgaaaaactg ttggaacaaa tccaactaga ggaagcagaa aaaaatcatc
     5101 cactgcttac tgctggtgaa atggatagtg tagtggtcca tcgtaaatcg cgtttatggg
     5161 aatttacgct acatttttcc aaaattttgc ctattatgct atatcggtca ttgactcagc
     5221 atctgactat cgcttttaaa gatattgcac aagtcaaact gaatatctta gcagatgacc
     5281 agacattcga tgaacagctc ttgcaagatt actgggccca agctttggaa aatcagcaat
     5341 gcgatacacc actagcacag caagtattga aaacgcaggt acctgtgata aaagatcgta
     5401 aagtcatctt gccaatcgac agtacaggag ctatttctta tttgaagcaa caatacttgc
     5461 cattagtaga agagttgttc gtcagttacg gattcccaaa atttcgtatc gaaccagaag
     5521 tggatgagca gcaagcggaa cgtgtattga aactttttga agaacgcaaa caagaacagg
     5581 cagaagcctt tatgaagcaa gcagctgaaa gtctagttgt ccacgaacaa aagaaaaagg
     5641 aaagaaaaga gcaatcgcca gctcttgaag gacccatcca gctaggacga aatattccag
     5701 ccgatgaacc aacgacacca atgatcagta tcgttgaaga agaacgaaga gtcacacttg
     5761 aggggtacgt attcgacaaa gaagtcagag aactacgttc aaaacgcaag atcttgactt
     5821 tgaaaatcac agattatact tcatcattta tcgtcaaaaa attctcgaac ggcgaaaaag
     5881 atgaacaagt atttgatgcg atcagtgtcg gtagctggtt aaaggtacga ggaagtattc
     5941 aagaagatac gtttgtccgt gatttggtca tgaatgcgca agatatcata gaagtcaaac
     6001 atacacctcg caaagattat gctcctgaag gggaaaaacg agtagagctt catgttcata
     6061 gcaatatgag tacgatggat gcaacaaaca gcatctctga tcttgtggca caagcaggaa
     6121 aatggggaca ccgagctatc gccattacag accatggtgg tgcgcaagcc tttccggaag
     6181 cacatagtgc tggaaaaaaa gcaggtgtga aaatcttata cggcgtggaa gcgaatgtcg
     6241 tagatgatgg tgtcccaatt gcttataatg atgcacatga agcattgagt gaagccacat
     6301 acgtcgtatt tgacgtggaa acgacaggat tgtctgcagt atatgacacg atcatcgagc
     6361 tagccgcagt caaaatgtat aaaggaaatg tgatcgaaag ctttgatgaa tttatcgatc
     6421 caggacatcc attgtcacga acaacggtag atctgactgg tatcacggat gaaatggttc
     6481 gagggtcaaa gtcggaagaa gaagtattgc ggatgttcct tgaattttca aaagatacaa
     6541 ttttggtagc ccacaacgcg gcttttgata tggggttctt gaatactagc tacgcaaggt
     6601 atggtattcc tgaagcggcc aatcctgtca ttgatacgtt ggaattagct agatatctgt
     6661 atcctcaatt caaacgtttc ggattaggtg tgttatctaa aaagtttggt gttagcttgg
     6721 aacagcatca ccgagcaatc tatgatgctg aagcaactgg tcacttagca tggatctttg
     6781 tgaaagaggc aatggacaat cataacatgc tttatcatga tcagctaaat gagcatatcg
     6841 gagaaggaga ttcttacaaa agggctcgac ctttccatgt aacgatttta gcaaaaaatc
     6901 aggcaggact gaaagatttg ttcaaactga tttcaatgtc gaatgtcgaa tacttcgaac
     6961 gcgtcccacg aataccacgc tcccaattga aaaagatgcg tgaaaatcta ttgatcggct
     7021 cagcttgtga taagggagaa atattcgaag ccatgatgca aaaaggggta gaagaggcaa
     7081 gaaaccgagc aaaattctat gattatattg aagttatgcc aaaagcggtc tatgcgcctt
     7141 tgatcgagca agaattggta aaaaatgaac atgatttgga agaaattatc caaaatttgg
     7201 tagaaatcgg aaaaagtttg gataaaatcg ttgttgcaac aggtaatgta cattatctga
     7261 acgaagaaga tgccatttac cgaaaaattt tgatcaattc tatgggcgga gcgaatccgt
     7321 tgaatcgtca tagtttgccg gatgtccatt tccgaacaac cgatgagatg ctaactgcat
     7381 ttcacttttt aggggaagaa acagcgaaag agatcgtagt agaaaatacg aataaaatcg
     7441 cagatatctg cgaagaagtg attccagtca aagatgaact ttatacaccg aagattccag
     7501 gttcagaaga tgaaatatca gaattgagtt acacaaaagc aaaacaaatg tacggagatc
     7561 cgttacctga gatcattcaa aaacgattag aaaaagagtt gaattctatt aatggtaatg
     7621 gcttctcggt tatttatctg atcgcccaaa aactagtgca taaaagtaat gaagatggct
     7681 atctagtagg ttcacgtggc tctgttggct ctagctttgt tgcgacgatg acaggaatca
     7741 ctgaagttaa tccattagcc cctcattatt attgtccaga gtgccaatat tccgagtttt
     7801 ttgaagatgg tacgtatggt tctggatttg acatgccaga aaaacaatgt ccaaaatgtg
     7861 gtgcacgctt aaacaaagac ggtcatgata ttccatttga gacattttta ggattccatg
     7921 gagacaaagt gcccgatatt gatttgaact tctcaggtga ttatcaagca gaagcccata
     7981 actatacgaa agtattgttt ggcgaagact atgtttaccg tgccggaacg attggtacag
     8041 tcgccgacaa aacagcatat ggctatgtaa aaggatacga aagggacaat aatctgcagt
     8101 ttcgaagtgc agaagtcgac agattagcaa aaggcgctac tggagtcaaa cggacaaccg
     8161 gccagcatcc aggggggatc atcgttattc ccgattatat ggatgtctat gattttacgc
     8221 cgatccaata tccagcagac gatcaaaatt cagaatggaa aacgactcat tttgatttcc
     8281 actcgattca cgataatgta ttgaaattag atattctagg gcatgatgac ccaactgtta
     8341 ttcggatgct tcaagattta tcagggatcg acccacaaac gatccctacg gatgacccgg
     8401 aagtgatgcg gatatttgcc ggtcctgaag tacttggagt aagtcaggaa caaatttatt
     8461 ccaaaacagg gacattaggt attccagaat tcggcactcg ttttgttcga gggatgttag
     8521 aagaaacgca tcctacaact tttgcagaac tactgcaaat ttccggacta tctcatggta
     8581 cagacgtatg gttaggaaat gcggaagaat tgattcgacg aggagatgcg acactggcgg
     8641 aagtaatcgg ctgtcgtgat gatattatgg tttatttgat ccatgctgga ttagatagcg
     8701 ggatggcatt taaaatcatg gaaactgttc gtaaaggaca atggaacaag atccctgatg
     8761 aattgcgaga aacctatcta tcggcgatga aagaaaataa tgtgcccgac tggtatatcg
     8821 attcttgttc aaaaatcaaa tatatgtttc cgaaagccca tgctgcagct tacgtattga
     8881 tggctcttcg tgtcgcttat ttcaaggttt atttccctat tttgtattac tgtgcatact
     8941 tttctgtacg tgcagacgac tttgatctag tctccatgtg caaaggaaaa gatgctgtga
     9001 aacaagcaat gaaagaaatc acggacaagg gattagatgc ttccgtaaaa gaaaagaatc
     9061 aactgacagt gctggagtta gccaatgaaa tgctggaacg aggattcaaa tttggaatga
     9121 ttgatttgta taaatcggat gccgttaact ttgttattga aggtgatacg ttgatcgcac
     9181 catttagagc tgtcccaagt ttgggaacca acgtggcgaa acaaatcgtt gaagcaagaa
     9241 aagacggtcc atttttatcc aaagaagatc ttgccacccg cggtaaagtt tcaaaaacat
     9301 tgatcgaata tatgaacgac aatggcgtat tgaaagatct accagatgaa aatcaattat
     9361 cgttattcga tatgctataa gcatctgttt tcctgtctgc ttgtaatttt aagcagagta
     9421 tggtatagtt cttataggta atgatactaa ctgtgagtga gcggattttt cgctcactct
     9481 ttttaatgga attacgcact taatttttaa ggaggcgaaa cttttgagta gcgtcgttga
     9541 aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac ttgtcgaagt
     9601 cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca aagaaggcgg
     9661 tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac ttgatgcagc
     9721 agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg gagcagagcg
     9781 gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc atgtctcctt
     9841 ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat ttgatgctga
     9901 acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt ttgaccgaaa
     9961 aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa tcagaaaaaa
    10021 atgagtaaag aaatgttaaa tgcgttagac gctttagaag ctgaaaaagg gatctcaaaa
    10081 gaaatcgtga tcgatgcatt agaagcagca ttggtttctg catacaaacg tcactatggc
    10141 caagcacaaa acgtcgaagt ggaatttgat cagaaaaaag gaaagatcca tgtctatgcg
    10201 gtaaaagaag taacggaaga agtgatggat tcacagttgg aagtctcatt gaaagatgca
    10261 cttttaatca atccagcata tgagatcggt gatacgatcc gttttgaagt aacaccaaaa
    10321 gattttggac ggatcgctgc gcaaacggct aaacaagtta ttttacagcg tgtacgtgaa
    10381 gcagaaagaa cgattattta taacgaattc agtgcttatg aaaaagacat catgcaagga
    10441 atcgtcgaac gccaggataa acgttatatt tatgtcaatc ttggtaaaat tgaggcagtt
    10501 ctttctaaac aagatcaaat gccaaatgaa ttttatcaac cacatgatcg aatcaaagtt
    10561 tatgtttccc gagtagaaaa tacgtctaaa ggtccgcaag tatttgttag ccgcagccat
    10621 ccagacctat tgcgtcgctt atttgaacaa gaagtaccag aagtctatga tggaatcgtt
    10681 gaaattgtca gcatcgcccg tgaagctggc gatcgttcaa aagtggcagt tcgctcaaat
    10741 gatccaaata tcgatgcagt cggtacatgt gtcgggccaa agggacaacg agttcaagca
    10801 atcgtcaatg aattaaaagg cgaaaacatg gatatcgtcg aatggaatga agatccagct
    10861 gtattcatcg caaatgcatt gaatcctgca caagtcatgg atgtgatctt cgatgagaca
    10921 aattcaaagg cctgcacagt tgtcgttcca gactaccaat tatcgctagc aatcggtaaa
    10981 cgcggacaaa atgcacgttt agctgctaaa ttgactaacc ataaaatcga tatcaaatca
    11041 gaatctgaca tgacggaatt ttatgaaaat caagaacaac aaaaagaaac agaagaactg
    11101 catgatgaag caatcgttca gtcagattta acagacgatg aatatgaaac aattgcattc
    11161 aacaatgaaa cggtcgaaga aaaaccagaa gcttaacttt agtaaggagg caaagagatg
    11221 aaacaaagga aaattccttt acgcaaatcc gtggtttcag gtgaaatgaa acctaaaaaa
    11281 gagatggtcc gaatcacccg ttcaaaagaa ggggaagtat cgatcgatcc aactggaaaa
    11341 atgcctggac gaggagcgta tgtctcactt gagccggaag aagtacaaca agcttgggat
    11401 aaaaaaattc tcgatcgagt actcgaaaca acattaacag atgagtttta tcaagaactt
    11461 cttgactatg tcactcacca aaaagcacgt aaagagttat ttggaaaatg aatacagtta
    11521 atcggcaaaa agccatgaat ctcatcggtc tagcgatgcg cgcgggaaaa ctaatcacgg
    11581 gtgaagaact gaccattgct gaaatccgca aacaaaaagc aaaattggtg tttgtggcaa
    11641 ccgatgcaag tgagaataca agaaaaaaga taaaagataa gagttcatat tatgaagttc
    11701 cttgctttga gctattttct gaagcagaaa tcacgcagat gatcggaaaa cctcgcaagg
    11761 tattcggaat aatggatgcc ggatttgcga agaagaccaa ggagctaatt gaaggttagg
    11821 aaggtgattg catgggcaac aaaagaatct atgaattagc aaaagaactc aaaaaatcta
    11881 gtaaagacgt cgtggaaaaa gcacaacaac ttggcatcga tgtgaaaaac catatgggtg
    11941 cgattactag cagtgatgag aagaaattgc aacaagcgtt caaaaaccca agcacgaaac
    12001 aacaagctgc acaacaagca agcagaaaac cagccaatca acagccgaac gatcaacgta
    12061 aaaaagaaac aacgaacaat caacaaaaaa ataaaaataa ccgcagttat caagatcgcg
    12121 gtcaagggag cggtcaagtg aatcaaggaa aaaaaccaac aacaaaccaa aataatacgg
    12181 gaaataccag cagccaaaat cgtcaaggaa cgagccaagg cggcaatcaa aaccgtacta
    12241 acaataacaa caaaccaaac aataacaatc gcggcaaatt taataataat aaccgtaatc
    12301 gttataataa aaaaggtaaa aaaggcaaac agcaacaatc aactaaacca gctgtaccac
    12361 cacgcaaatt ccgtgaattg ccagatgtat tagagtatac tgaaggtatg aacgtagcag
    12421 atattgcgaa aaaaattcat cgtgagccag cagaaatcat taaaaaatta tttatgcttg
    12481 gtgtcatggt caatcaaaac caagcactgg ataaagatac gattgaactg ttagcgacgg
    12541 actatggcat ggaaccacaa gaaaaagtcc aagttgacat tgctgatatc gataaattct
    12601 tcgaggcaga agaagtcgat ccagataaat tagtctcacg tccgccagta gtaacgatta
    12661 tgggacacgt agaccacggg aaaacaacct tattggatac attgcgccat tcacgtgtaa
    12721 caagcggcga agccggcgga atcacacagc atatcggtgc ctaccagatc gatattgatg
    12781 gtaaaccaat cactttcttg gatacaccag gacatgcggc ctttacaagt atgcgtgcac
    12841 gtggagcaag tatcacagat atcacgatct tagttgttgc tgcagatgat ggcgtcatgc
    12901 cacaaacagt tgaagcaatc aaccatgcca aagctgcagg cgttccaatc atcgtcgctg
    12961 taaataaaat tgataaacca ggtgcaaatc ctcagcatgt tatgcaagaa ttaagtgagt
    13021 atgaattaat tcctgaagca tggggtggag aaacgatttt tgttgagatc tcagcgaaat
    13081 tcggccaaaa catcgaagaa ttgctggaaa tgatcctgtt agtcgcagaa gtagaagatt
    13141 taaaagcaga tccaacacaa cgcgcaatcg gaacagtgat cgaagcacgt cttgataaag
    13201 gtaaaggtcc tgtgtcaacc ttacttgtcc aagaaggaac acttcacgtt ggtgacccaa
    13261 tcgttgtcgg taatacttac ggacgtgtcc gtgtaatgac aaacgatctt ggccgtcgcg
    13321 ataaagaagc aggacctgca acgccagtag aaatcactgg cttgaatgat gtacctcaag
    13381 caggtgaccg tttcgtagtc ttcgaagacg aaaagacagc acgtgcagcg ggtgaagaac
    13441 gcgcaaaacg tgcattattg gaaaaccgtg ctgccacaag ccgtgtgact ttggacaact
    13501 tatttgaaag cctgaaagaa ggcgaattga aagaagttaa cgtaatcatc aaagcagatg
    13561 tacaaggttc agcagaagca ctggctgcat cattgaagaa gattgatgtg gaaggtgtcc
    13621 gtgtaaaaat cgtccattca gctgtcggag caatcaatga aagtgatgta accttagcag
    13681 cagccagcaa cgctattatc atcggtttca acgttcgtcc tacaccgcag gcaaaagtgc
    13741 aagctgatac tgaagaagtc gatattcgtc ttcaccgtat catctataaa gccatcgaag
    13801 aaatcgaaac tgcgatgaaa gggatgctag accctgaatt tgaagaaaaa atcaccggtc
    13861 aaatgatcgt tcgtgaaacc ttcaaagttt ctaaagttgg tacgattgca ggtgcctatg
    13921 taacagaagg atatattcgt agagatagcg gtgtccgtgt aatccgtgac ggaatcgtaa
    13981 tctatgaagg ccaacttgca agtctgaaac gattcaaaga tgacgtgaaa gaagtcaaaa
    14041 tgggttatga atgtggtgcg atgattgaaa aattcaatga catcaaagta gatgacgtaa
    14101 ttgaaggatt tatcatggaa gaagtcaaaa atgactaaaa actgataaaa ggaggccaat
    14161 cattatggct aactaccgtg atcgccgagt cggtcaagaa atcttaaaag aagtcaatga
    14221 tattttgcgc aaaaaggtcc gtgaccctcg tgtggagaac gtaacgatca cagatgtgaa
    14281 agttacgggg gacttacagc aagcaacgat ctattacagc attttatctg acttagcatc
    14341 agataagaaa aaagcacaag atggattgaa taaagcatca ggattgattc gtcgagagtt
    14401 agggcacaat ctaagtattt ataagacacc tgaattgttc tttgaacttg atgaatcagt
    14461 tgtctatggg aaccatatcg atgaattgct tcgcgacttg aacaaagact aatcaaaaaa
    14521 tcccgcaaat cctgtgaaat caggggttgc gggatttttt tgttttcatt aagacgtatt
    14581 acctaagagt tccatgactt tagtctggta gcgtgtcagc taaccaaaaa agagaaaaag
    14641 aaaagtaaaa aattcgactt tcttttttct atagtatata aatttttgca tatagataaa
    14701 gatcaagata ccgataacgg aagctagatt gtaatctatt ttagtgacag tcaggtatag
    14761 ccaagtaact agaagatcag tggaacctgc accataaaag tttgagtttg tcggtccgtt
    14821 gacagttaaa agaaaaatca cgttgaagtt attgatattt ccgattacgc cgcttcgatt
    14881 tggtcacgat ccaaattttg gataatccct gtagaaatta gcatagtagc tgggatacca
    14941 attcacatat tgacaaagat aacagagact tttacataaa acggatcaat caggaaagga
    15001 attgctcgat ccagccccag ttttccaaca atgtgttaac tggcctagcg tcattcagca
    15061 aattatgcat gatgaggagc gaaatgaact gcggaacagc cattgtgata acgacaagtt
    15121 ttttggatca atgtaaaaaa cgtcagcgaa aggaaaaatt tcgtggtttt ttatacctgt
    15181 tcatttggct agatataaaa tccaaacaaa tgaacagaag tgcttattag gtttcaattg
    15241 ggagtatctg cgctagaata agagtaataa tgaaaaggag tgttatgtat ggattattct
    15301 agtaaagaat actttgataa aatgacggct tggtggcgtg ctgctaatta tctttctgtt
    15361 gggcaacttt atcttaaaga taacccctta ctgagacgta cactgaaacc cgaagatgtc
    15421 aaaaaacatc caatcggtca ttggggaacg attcctggcc aaaattttat ttatgtccat
    15481 ttgaaccgag tcatcaataa atatgatttg aacatgtttt atattgaagg gccaggacac
    15541 ggcggacaag tcatggtatc gaatgcttat ttagatggaa gttacacaga gatctatcca
    15601 gaagtaacgg aagatgagac aggaatgcaa aaattattca aacgattttc atttcctgga
    15661 ggcattgctt ctcatgcagc accagaaaca ccaggttcta tccatgaagg cggagagctt
    15721 ggatactctc tttcacatgc agtcggagct gtattagaca atcctgaagt aatctctgct
    15781 gtcgtgattg gtgatggtga agcagaaacc ggtcctttag ctggaagctg gttctcaaat
    15841 gtctttatca atccagtgat agatggagct gttttaccga tccttcattt aaatggtgca
    15901 aaaatagcca acccaacgat cttggcacgt aaatcagatg gagaattagc aaattacttt
    15961 aacggacttg gctgggaacc tttctttatt gaaggaaatg attcagaaaa attaaatcct
    16021 gttatggctg aaaaaatgga tcaagcaatc gagaaaatta aatccatcca aaaagaagct
    16081 cgcctgaaga cagcaacaga tgtcgtgatg ccaaaatggc cagtattgat cgtacgcaca
    16141 ccaaaaggat ggaccggtcc aaaagaatgg gacggggaac caattgaagg aaccttccga
    16201 gcacaccaag taccgattcc tgtagatcaa gaacatatgg atcatgcaga tgctttactc
    16261 cgttggctga aatcctatga accagaaaaa ttatttgatg cacaaggacg tattttagaa
    16321 gaaatcagag aaatcgcgcc tactggtgac caaagaatgg caaaaaatcc tatcaccaat
    16381 ggtggaatcg atccaagacc gcttattatg ccagattgga aaaaatatac tttgcaattt
    16441 gaaaaaccag gctccgttaa agcagaagat atgaccgaac ttggtaaatt cgttcgcgag
    16501 atcattgaga aaaatccaga aaatttccgt atatttggac cagatgaaac aaaatcaaac
    16561 cgattgaatc aagttttcaa aacgacaaat cgccaatgga tggaaaaaat cgaaccagaa
    16621 aatgatgaat ggctttcgcc atctggtcgc gtcatcgaca gtcaattatc ggaacaccaa
    16681 gatgaagggt tcttagaagg gtacgttctg actggacggc atggtttttt gcaagttacg
    16741 aatcattctt gcgagtggtt gactctatgt tgactcagca cttcaaatgg atgcggaaat
    16801 cacatgacct ttcttggcga aatgattatc cttcgctaaa tctcattgca agctcaacag
    16861 tcttccagca agatcacaat ggatatagcc accaagatcc aggaatattg acacatttgg
    16921 cagaaaagaa ggcggagttc atccgtgaat atctgcctgc ggatgcaaat acattgctcg
    16981 cagtcatgga taaagcattc cgctcgtcag aaaaaatcaa tttgatcatc tcttccaaac
    17041 atccgcgtgc tcaattttac tcagcagaag aagcagccgt cttagtgaat gaaggcttga
    17101 aaatcattga ttgggcttcg acagcaaaag aggaggaacc tgaattggtc attgctgctg
    17161 caggaacgga atctaactta gaagcattag cagctgtaac tttgctgctg gaagaattcc
    17221 caaaactaaa aattcggttc atcaatgtcg tagatttatt gaaattacgt catccaagtc
    17281 aagatccgcg tgggttgagc gatgaagaat ttgatcaata ttttacaaaa gacaaaccga
    17341 tcttatttgc tttccacggt tatgaaacat tggtccgcac gattttcttt gatcgtcata
    17401 accatcattt aatgattcat ggctacaaag aaaatggaga tatcacgaca ccatttgaca
    17461 tgcgtgtagt aaatgaactt gatcgttatc atttggcaaa agacgcagct ttaaaaatca
    17521 aaggaagtca agcagaagat tttgcgaaaa aaatggatca gaaattacaa gaacatcaaa
    17581 actatattcg tgaaaatggt attgatctgc cagaagtatt ggattggaaa tggaaaaatc
    17641 ttgaccaata aaatgataaa atcgatcccg caaaatagaa ttttttacat tgattaagaa
    17701 actgaaaaaa taaataaaaa aaacggaaaa aagactggaa gaatggccag tctttttttt
    17761 cgtttttggg aagtgaaaac caaactattt acctgtaagc cacccataaa tccaatcaaa
    17821 tggtgcaaag aaaaaatctt ccattctatt ccctccttta aaaaagcata acatgatttc
    17881 tatttacgtg agcatgaatt ttgttacgtt tttattgccg aaaaataaaa accgttcatt
    17941 atgatgtcaa attcgtcagt aattgatgca atgtgttata ataattcagt taaatcaaaa
    18001 atgaaatgga gaaaagacat ggacgggtta ttacctttat ggaaagaacg cggaatgaca
    18061 agtcatgatt gcgtattcaa attaagaaaa atcttacata cgaaaaaaat cggacatggg
    18121 ggtacgcttg acccagacgt agacggtgtg ttgcccattt gtattggaaa agccacaaaa
    18181 gttatcgaat atttgacaga ttctgggaaa atctatgtag gcgagataac tctaggtttc
    18241 agtacaacga cggaagatcg atccggagaa cgggtagaaa caaaaccagt agatatgtct
    18301 ttatctgaaa aaaaaataga tgaagtgatg gcttcattta ttggtgagat cacccagata
    18361 ccaccaatgt actcagcggt aaaagtcaac ggtaagcggt tatatgaata tgcccgcaat
    18421 aatgaaacag tggagcgtcc tgttagaaag gctcagattt accgttttat ccggacaagt
    18481 gaattgacaa gagacgaagt gacaggaacc atttcttggc gcttcgaagt agaatgcggg
    18541 aaaggaacct atgtccgcac attagctgta gatacaggca agaaacttgg ttaccctgcg
    18601 cacatgtctg atctgacccg aacagcaagt gccggtatgt cccagaagga tgctctgaca
    18661 ttagaagaag tagctcaagc tgtgaaaaat gggacaatcg atgattgttt gctgccaata
    18721 gaaaaaggag tggaacggtt caaacgagta gatattactg atgaagtatg gcagaaagta
    18781 aaaaacggta tgagacttga ttatactgta tttgcgctcc cagaaatgcc gtcagaagaa
    18841 attgctttgt tttataaagg aaaagttgtc agtatttatc agccaaaccc gaaagaaaaa
    18901 aacaaattaa aacctagtaa agtattgaga aatgaggaat aacgaatgaa tatcatcaag
    18961 atcagacatc cttaccaatc agaacaaatt ccagaagacg atgtcgtttt agtgctggga
    19021 tttttcgacg gcgtgcatat cggacaccag aaagtcataa agacaggtat ggaaattgca
    19081 caaaaagaag gattgaaatt agcattgatg actttcaatc agcatccatc tattgtcttt
    19141 aaaaagatcg atcctagcag cgtgaaatat ttgactagcc ttaagcaaaa agaagaaaaa
    19201 atggccgctt tgggtataga ttatttatat gaagttgatt ttacttcttc ttttgctcat
    19261 ttagcaccgc aggaatttgt tgatcaatac atcgtagggt tacatgcaaa atatgctgtt
    19321 tccggttttg attatacgta tggaccaaaa gacattgcag atgtcgctca ttttcctgaa
    19381 tatgccaaag gaagattcaa aatcgtcaca gtttctaaag aagaagaaca aggtgaaaaa
    19441 atcagctcta cccgtattcg tggattacta gacagcggac aagtaaaaga agcagcaaaa
    19501 ctattaggtt gcacatatga ggtagaaggg gtagttgtcc atggcgatgc acgaggcaga
    19561 ctactaggat tccctacagc aaatgtcaaa gtaaagagca cggttcattt gcctaaagaa
    19621 ggtgtttatg tgtcggaaat aaaaattggg gaaacatggt atccagccat gggttcaatt
    19681 ggacataatg atacattcgg tgaaggacgg gagctgactg tagagttgta tattttggac
    19741 tttaatcaag atatctatgg tgaacatgtc gcggtacgct ggaatcaatt tttaagagat
    19801 caagtgaaat tttctagtgt agaggaattg attgaacaac tcaaggagga tgaacaagca
    19861 acagctgaat atttttctca gcaataaaac attatgcata aaatctcttg tttatgtata
    19921 actagcaatt acaataaaat gggtgataaa taagcccagg aaaggggctg tgacaaaagt
    19981 cttgtcacag cctttttttc caaataaatg gtttttcaga agtagcttct tcggaaataa
    20041 gccgaaattt cttgaagctg accacttctg tcacaacctc tttctttata gaaataaaag
    20101 gacactagca attcatgaaa acgattgcta gagcgtatca tttgctttag actatatatc
    20161 ttggtgtaca ctcactttaa ggcggaaata aaagttatcc gtaataacag gtttaggtat
    20221 gaaaaaggag tgggtactat gtacaaggaa ttagaaaaca aagtcgcagt agtaactggt
    20281 ggctcaaagg ggattggaac agcgattgct caacgattcg ggaaagaaaa aatgaatgta
    20341 gttgtcaact ataactcgga taaagaagga gcagaaaaag cagtcaaagc aatcgaagag
    20401 gcaggcggaa aagcagtctc tgtacaagca gatgtcagtt ctgaagaagg aatacagcgt
    20461 ttacttgata cggctttaga aaattttggg acactggacg tttgggttaa taatgctggg
    20521 atggaaaatc agcatccaac ccatgaactt agccttgaag attgggaaag agtgttaaaa
    20581 gtcaatttga ctggcgtttt tatcggaacg aaagcggctt taaattactt tacagagaat
    20641 aataaaaaag gaaatatcat caatatgtct tctgttcatg aacagatccc atggccaacg
    20701 tttgctcatt acgctgcttc aaaaggcggg gtaaaattat ttaccgaaac ggtagcaatg
    20761 gagtatgcac cgaagggaat cagaatcaat agtataggcc ctggtgctat ccgtacacct
    20821 attaatgcgg caaaatttga tgatcctgaa caaaaagaac tcttggaaag tatgatccca
    20881 ctaggacgcg taggtgaacc aaaagaaata gcggcgacag ctgcatggtt ggcttcagac
    20941 gaatccagtt atgttacagg tattacacta tttgcagatg gtgggatgac tttatatcct
    21001 tctttccaag gtggaaaagg ataagggtga ataaaaatgg gtttattgat tgcattgatc
    21061 ccggcagttg gctgggggat ccagccactg attttgaaaa agattggtgg cagaccaaca
    21121 aatgagatat taggaactgg tattggagcg gtgcttgtcg gcttactcgt tcaattattc
    21181 gcttctccag ctggtatttc agtgactaca tttttgatta gtttactttc tggtgcattt
    21241 tgggttattg gacaaacagg acagtataca tctttcaact tgattggtgt ttctaaaacg
    21301 atgccaattt cgactgcatt acagttagtc ggaacctcgt tgattggtgt cttcgctttt
    21361 ggagaatggt ctggaagtat ggggaaaatc attggagcag cagcaatcat tcttcttgta
    21421 tttggttctg ctttaacggc aatcagtgat gatcctggaa aaaaaggcgg tatcacaaaa
    21481 ggaattagtc tattagcatc aacaagtatt ggttattggg tatacagtgc attgcctaaa
    21541 ctagttgatg caagcggact ttccattttc tttccgcaaa tgatcggtgt ctttttaggg
    21601 gcagtgattt atgtgatttt ccgtaaacca accgcatttt ctgaagacaa gagttggaaa
    21661 gcaacgatcg ttggagccat ctttagtgtg tcagcattgg cttatatttt ttcagcaaat
    21721 gaaaatgggg ttgctactgc atatatcatt acacagctaa acgtagttat ctctacatta
    21781 ggcggtatgt acatcctgca tgaaaataag acaccaaaag aaaaaaatta acgattatcg
    21841 gattattgct gatcgtagta ggaagcatgg tcacggtctt tatttgagga taaagatggt
    21901 aaaatttcat ttattaaaaa aatatttgtt tgaaccaaac agtaagtact tataaaaaaa
    21961 tagcgctaga atttccaaca tctctggaat ttctagcgct ttttaattgt ttaagataca
    22021 agggaaatat ctgtctggat caataatttt tcattgatcc attgactaaa gtttacgaca
    22081 taattgaagt taccaccgat tagtatctcc gcatgattgt ctttataaag atcgtattgt
    22141 aaaagacggt cttttttatt tgcgtaaaca tcgttatggt actggactaa ttgttctcgg
    22201 aattgattga ttgctgtaaa atcacgatat tgaaggttga aacgcatcgt gattggtgca
    22261 tttagcatga atcgctgcat ttcttttgct gcttcctctg cttgatctcc gtataggaac
    22321 tcaaaaagat tacgttcaga aaatttccat tgcccgccaa cctttgttcc ttttagtttt
    22381 ccttcatgaa gataatttct gatagaacgt gtagtaacac ctaattgttc ggctaattca
    22441 tttactgagt aaaaatcttt caatgttcac cctcctatgt gaatggttct actgacttag
    22501 ttgatatttg cttaatcacg aaatgtagaa agaacgattt taattaaggt cattgtaaaa
    22561 atcaagtcag aaaattctaa cttttatttc tctaaagaaa ttcgttctct cctaaagtga
    22621 tattatattt aaatatacct gaaaataatg ggcagtatta tgaaagatca ctgatatttt
    22681 ctcgtatttt ttccttattc aaaagttttg caggaaaatt gctgaaaaaa taattttact
    22741 aagtagaaaa ttcaaagcca ttcaatcagt gatttttttc agcacaagaa agactcctta
    22801 taaaaatagc agatactttg tcaattaagt gtttaaccag ggatgataaa aaggatagga
    22861 ttgaacaaaa cctgtataag gtgatgaata gtttcctcgc cagtaaaagg tttattcgta
    22921 taaagcttta tcgccaaaat ttatcatgac cttttttatc ttcaatatta aatgaaatca
    22981 attgttcaat caattcccag ttgattacac ttttccaagg aactttgatg acgttatccg
    23041 tatatacgta gcctgcttct tggatatttt tttcaagggc acgaatcgct gctttttccg
    23101 gtgcgataga aaaatgattt ttagcaaaac taaatgcaat aataaaggta ccatggtcag
    23161 taaacatagg ttggttccat tttacagttg tgtctagctg gggatacgtt tctgttatct
    23221 tagcaaaaag gtctttgatc ttttgttgat gttctttttc tttgatttga ttgatgatcg
    23281 gctgaaattt ttccattcta aaacgcctcc attaagtggt ttgatttatt ttaccagaaa
    23341 aacaatagtt atgtataaaa aacgaacaaa acacaagtaa tgttgtaacc gctatattta
    23401 tatgataaga tgtctcaaat aataaaatag aaaagagaca taaaaatgat tgacttggca
    23461 aagaaattct tacacgaaaa tatatatttt gagttgtatc atcatcgagc catttataca
    23521 aatgaagatg ctttggtaat aaagaaagaa caaggattta caggtacaga gaccaaaagc
    23581 ttattcttaa aagataaaaa agataataac tacgtatatc tgacttttac tacgaaacaa
    23641 acggatttca aagcattagg gaagatcgtc ggtaaaaggc tttctattgt ttcatcagaa
    23701 cgcatggaag aacaaacggg acaaaagcag ggcgctgttt ctccgtttgg ttatgaaaaa
    23761 cagattccga ttattattga tgaagagctc ttatcaaatg aaaaacttgt ttttgcacca
    23821 ggtagaccag atcaaacgat ggtcatccaa acggttgact taaacaaaat catccagcta
    23881 cttgagatgc cgatattttt gatgccggca aacgaaaaaa acaattaacc aagtaaaata
    23941 gtgcatactt ggaggtgaag caacatgata agttatccat tattagaaag aaatgcaacg
    24001 attggggtta ctgcaccatc ctcaggcgta tcagaggaac accattcgtt actcgaatca
    24061 gctttaaaga gaatggaaga aaaatctttc caagtagtta ctggaaagac gctttggacg
    24121 caagataagg aaaaatcagg tccagcaaga aaacgagcag atgaattcaa tcaaatgatg
    24181 caagatgaaa atatccaaat gatctttccg acttggggag gagaattatt gattgagatt
    24241 ctcgattttg tcgattttga tgctctacaa ccaaaatgga tagtaggtta ttcagatatt
    24301 agtacgttgc tacttgctgt cacattaaaa acagggatag ccactgcaca tggaactaac
    24361 cttattgatt taagaggaga gtacttggat gaaacgacag caatgtggga aagtgtactg
    24421 aaaacaagag aaggagaatc gattctacag caatcttctg aaaagtatca aaaagtgtgg
    24481 ccccatgagc attcatcatc ttctgttttc catctcactg aaccaacaaa ttggcaatca
    24541 gtcagcggag atgcagtaag gatgaaggga cgattattag gtggttgtat tgatacgatc
    24601 aggcatttaa tcggtactcc ttatggagat gtccaatctt ttaaaagaaa gtataatgat
    24661 ggaaagccga tcttgtggtt tctagaaaac tgtgagctat cgtcagttga tttaagaaga
    24721 tcacttgttc agatgaaact ggcaggctgg tttgatggca gctcggggat catgttcggt
    24781 agaagtgcgg cagaggattt gttagattat aaaatgaagg atgtatacga agatctgtca
    24841 aaggaattaa aactaccgat cttgtatgat attgattgtg gtcatgtacc gccacaactg
    24901 acattgatta atggtgccta tgcagaagta gcggtagaag atagaaaagc gactgttttg
    24961 caaacgttta tttaaaaaat tttttcctgc cttggagtct atcaaagacc aataaatcta
    25021 aatagaaaag aaagtgaaag aagctgacaa aatgctgtca gcttcttttt gtataacata
    25081 ttagaaagtc taattatcta taactgtcac atcgattaac tgtttcacgt cattgggatt
    25141 tcctgtagga atagctacta cagatgcatt atcaatgatt tcttgagttg tttctctaat
    25201 gaacgctgga taagatgttt gatctgcagg catttgccaa tgtgaaggaa ctggataact
    25261 agtgattgtc ccgtcatggt tacttgaata cactaaaact ccctggtaac tgtatttccc
    25321 agtgagcata accacatctt tcggatagac tgcactccct gcatcataag gggcgatagg
    25381 tgttcctgca ggataatgaa taacatgtaa ttcgaaatca cccgatgtat ctctaaaagt
    25441 attacctcta atagctagcc aaacacgtgc gtactcaatt tgtagatcag aatatcctgc
    25501 taacggatct attgactgag aagactgtga ggaggttgtg tctattgttg aagaagaaat
    25561 atttgtctca actgtactac tagatgtaga agaagtattg ggctctgaga ctttactgct
    25621 tgttgaagat gtattcgttt cagtagaagc tgatttctga gtacttgatt tttcagctga
    25681 actggtttgt ctgattgaac ttttgtctgt ggtcgcagat ggatcactat tttctttact
    25741 aggagacgta catcctacaa ggaataaaga gaccaacaat aaaataaaac ctaacttttt
    25801 catataaaat acctcattat ttttttaata ccagtatagc ataatcctga atttttgaat
    25861 ccgtaaaacc ttgtctatga atataaatag agtgtatctt ctttgataaa tgtcagaaaa
    25921 agtgtgacaa ataatcatca gtaaaattac ttgtcacact attgataagg agtatttaat
    25981 tgaagagaat gattaggaga aaggatctcc gtacttttta tagtaatcat agaatccaag
    26041 tttgtataga cgaatcgttt tttctcttat ttcttctgtt tcaaaatgga ctgcttgaag
    26101 aatcaagtct gtgaattcta taggagctgt tccattcgca gtaatcaaat tacgatcttt
    26161 cacagcttgt tgttctataa aatcttcttt attctgataa ttttccgatt ctaaccaagg
    26221 aaattgcgca tttccggtgt gtttgtaacc ttgcaataat ccgctctgag ctaaataatc
    26281 aactgcccca caaatagcag ctactggtac gtctgttttt aacaatttat cgattatttg
    26341 atataaatga tgatttttta tgtcccatga attgccaccg atcataataa aaaggtcaac
    26401 tttttcaggt atttgttcga ttaagtaatc gatttttgtt tttaatcctc caatggaagt
    26461 gacctctgat tggatagaag cagttttgtt ttgccatttc tcattttggt ttagtgtgct
    26521 agtaatataa gcaccttccc aatcggcata ttcctctaat agaaacgtaa tcgcagtttt
    26581 cattttcaag ctctccttta ctatctataa caaggacaac agttttaacc tacagacatt
    26641 cattaaaatt gggtagcgac agcgaaaaca acaattctta agttttagac agagtataca
    26701 gatagtcaac ttaattaaat acgaactaaa taaaaatgac aggtaatcat ttttgtttac
    26761 cagcattata acattttgca tacatggatg acaaaagaac caattcccac ttgcttgagt
    26821 acaatattaa aagaaactgg aaggcaaaaa gtaattatag aaacgaaaaa taaatcatat
    26881 cgggcactca gacatgaaaa caaccctaga gatatataac taggtaacca gtttaatgaa
    26941 agaaaaaatc atccaagaag tcgattcttg gattttttag tggatttgtt cgccaaatac
    27001 gtttagtatg cgtatatgag aggttgtaag caaagcgttg tggtctgagc acttaaggag
    27061 gaatctgtga aaatagcttt tcatattttt gcagattttg acttaatgcc gaaggtcagc
    27121 tttgcgatta ccgtttatcg agaggctgta aaagcaccgt gtagctccga tgacaataag
    27181 caagcatttt tagagctagg tttccttgat tctaaatatg cggcttattt caatgagttg
    27241 gtgcttgaac gcgattttaa gaaggtcgaa aacaggcacg acttttcgac gtaaggagaa
    27301 agaatttcaa tgatagaaac aataaatccc gcagaaaaca aggtttctgc gtacattcat
    27361 atcccatttt gcgagcatat ctgctattac tgtgatttta ataaagtttt tttagaaggg
    27421 cagccagtag atgagtatgt ggaaatgctg ctgaatgaaa tgaaaataat gctagaaagt
    27481 catccgacag cgaatttaga aacactttac gtagggggcg ggacaccaac ttctttatct
    27541 gctaaacaac ttgatcgact gttatcaggt gctagagaga tcctgccttt tcaaaatggt
    27601 aatgagttta ctgtagaagc gaatccaggt gatttgaccc gtgaaaaact tcaagtaatg
    27661 aaaaactatg gcgtcaatcg tttatccatg ggagtgcaga catttgataa tcgcttattg
    27721 aaaaaaattg gacgtaaaca caccgcagaa gatgtgtatc aaactatgca atttttagaa
    27781 gcagaaaatt ttaccaatgt aagcatcgat ttgatttatg cattaccggg acaaacacta
    27841 gaaggatatc tggatacttt ggaaaaagct ttggctttgg atctgcctca ctattctttg
    27901 tactcattga tcctcgaaaa caaaacaatg ttcatgaact gggtacgaca aggaagactc
    27961 gaactgcctg atcaggaaac ggaaaccaga atgtttgaag agacgattga agcaatggaa
    28021 aagcatggac gtcaccaata tgaaataagt aactttgggc tctccggaca tgaatctcaa
    28081 cataacctca tgtactggaa caatgaccac tattttgggt ttggagctgg tgctagcggt
    28141 tatcttggag atattcgtta ccgcaacaaa ggaccaatcc aacactattt acgcccatta
    28201 cgagcaaatg aacgcccggt attggaagaa gaaaaattga cacgaaaaaa tcaaatagaa
    28261 gaagaaatgt tcttaggact aagaaaaaaa acagggatta ctaaaaaaca tttctatgat
    28321 cgctatcaaa aaacaattga atcggtgtac ggtgatgtgc taagggaatt agagcaagaa
    28381 gggttacttg ttaatgaaga agaccgtgtt tatttgacac caagagggac ctttttgggg
    28441 aacaatgtgt ttgagcgctt tttgtttgaa aaatagttta aagaatattt gttttttatc
    28501 tgaatttaaa taaaaaaagt tcaaaaatac aataaaggac tattgaaacg ttgatataac
    28561 tggatttaca tcaattattt agaattagca ctcttgtgtt tagagtgcta aaaaatggag
    28621 tctttctctt gacaggagaa gggcttcttg ctatatttat aagtggatta gcacttagaa
    28681 tatatgagtg ctaatgagag gtgaggaaga tgttaacaaa gcgacaaaac gacattttac
    28741 gtctgatcat ccaacactat acgagtagtg gcgtgcccgt tggttccaaa acattgatgt
    28801 ctgagggagt caaggccagc tcagcaacga ttcgcaatga tatgaaagca ttagaagatg
    28861 aaggattgct gttgaagact cactcttctt ctgggcggat tccatctgct ttaggctatc
    28921 gttattacgt ggatcatttg ttaaaacctg ctcgcgtagc agaagatgat ttgctgctga
    28981 ttcgtcagtc tctaggcaga gaatttcatg aaatcaatga gattattaag caatctgctg
    29041 agatattatc tgagctgaca agctatacga ctttttcatt gggacctgaa ataaaagacc
    29101 gccgactgac tggctttcgt atcgtaccct tgaataatcg acagattatt gctatcgtcg
    29161 taacagatat ggggaatgta gaaagccaag tgttcactct tcccgaatat atgggaagtc
    29221 aggaactaga aaaaatggta cagattgtaa acgaccgtct agtcggagat cctctgatga
    29281 cggtgtacca taaacttcgt acggagattc cgatgattct tcataaatat ttccaaacga
    29341 cagaagggat gtctcagtta tttgacaccg ttcttggtca tgtttttgaa gataaggttt
    29401 atgtcagtgg acaaatgaat ctcttggatt atgagccccg tcaagatctt gatcagttca
    29461 aatcgatgtt ttcttttatg aaagactcag acggacttac acaaatgatc actccgatgg
    29521 atagcaatat tcatatcaag attggtgctg aattaggcaa tgatttattg cagaatatga
    29581 gtatgattca agcaagttac gagatagatg gtcatggaag aggtacaata gccttgttag
    29641 ggcctactag tatgccttat tctcggatgt tcggtctagt cgatgtttat cgaagagaac
    29701 tagcagctaa acttgctgat tactaccgtt cgcttgactt gtcaaactcg taatgtgaat
    29761 aacgaagcta tttgcagctg atcgttctct ctttcttagt aagagcaatc aaacagctga
    29821 ctgaataatg aaaggaagac tgtatcgtga aagaaaatca agaagaacta gataaagaaa
    29881 tgacagatgc tcagccagaa ccggaaatcg atgtcgaagc tgcagaagat tcgggtattt
    29941 cagaagctga agcggaagaa ttcgaaacag cgaaattaaa agctgagtta gaagaaatgg
    30001 aagataaata cttgcgggct cgtgctgaaa tcgcaaatat ggcaaatcga ggaaaaaatg
    30061 agcgtgagca attacaaaaa taccgttcac aagatttagc caaaaaatta cttccatcaa
    30121 ttgacaacct ggaacgtgca ttagccacag aagtgtcaga tgatcaaggg gcaggtttga
    30181 aaaaaggcgt ggaaatggtc ttggaaagtt tgcgtaacgc actggaagaa gaaggaattg
    30241 aaaaaatccc tgctaaagga gaagcctttg atcctaatct tcatcaagca gtacaaactg
    30301 ttccagcgac tgaagataca ccagccgata cgattgtaga agtactacaa gaaggctaca
    30361 aattacatga tcgcgtgtta agaccaacca tggtcattgt tgcacaataa ttgtgcggat
    30421 ataaaactta cactttcaat taaagaagga gacgttaatt atgagtaaaa ttatcggtat
    30481 tgacttaggt acgactaact ctgcagtagc agtattggaa ggaaacgaag caaaaatcat
    30541 cgctaaccca gaaggaaacc gtacaacacc ttctgttgta tcattcaaaa atggagaaat
    30601 ccaagtaggg gaagttgcaa aacgtcaagc tgtgacaaac ccaaatacga tttcatcaat
    30661 caaacgccat atcggtgaag caggttacaa agtagatgta gaaggtaaat catatacacc
    30721 acaagaaatc tcagcaatga tccttcaata tatcaaagga ttcgcagaag actatttagg
    30781 tgaaaaagtc gaaaaagcag ttatcactgt gccagcttac tttaatgatg cacaacgtca
    30841 agcaacaaaa gatgctggta agatcgctgg tctagaagta gaacgtatcg tcaatgaacc
    30901 aactgcagct gctcttgcct atggtttaga taaaacagat cgcgacgaaa aaatcttagt
    30961 attcgacctt ggtggcggta cttttgacgt atctatcctt gaactaggag acggtgtctt
    31021 tgatgtattg tcaacagctg gcgacaacca tctaggtggg gacgactttg ataacaaaat
    31081 catcgactac atggtagcag aattcaaaaa agaaaacagc attgacttgt ctcaagacaa
    31141 aatggcttta caacgcttga aagacgctgc tgaaaaagcg aaaaaagatt tatctggcgt
    31201 atcaagcaca caaatcagct tgccatttat cacagcagga gaatctggac cattgcattt
    31261 ggaaatgaca ttgacacgtg caaaatttga tgaattaaca gctgacttag tagaacgtac
    31321 aaaagtacca gtacgtcaag cgctaaaaga tgcaggttta tcacaatcag aaatcgacga
    31381 agttatttta gtcggtggat ctactcgtat ccctgcagtt gtagaagcag tacgtaaaga
    31441 aacaggaaaa gaaccaaaca aatcagttaa ccctgatgaa gtagttgcta tgggtgctgc
    31501 tatccaaggt ggcgtgatca ctggtgatgt gaaagatgtt gttttacttg acgttacacc
    31561 attgtcatta ggtatcgaaa caatgggtgg agtgttcact aaattgattg accgcaatac
    31621 aacgattcca acaagtaaat cacaagtatt ctcaactgcg gcagataatc aaccagcagt
    31681 agacatccat gtattacaag gtgaacgccc aatggctgct gacaacaaaa ctctaggaag
    31741 attccaattg acagatattc cagcagctcc tcgtggtatt ccacaaatcg aagtaacatt
    31801 cgatattgat aaaaacggga ttgtaaatgt atctgcgaaa gatctaggta cacaaaaaga
    31861 acaaaaaatc acgatcaaat cttcttcagg tctaacagac gaagaaatcg aacgtatggt
    31921 aaaagatgca gaagcaaatg cagaagcaga taaagctcgt aaagaggaag ttgacctacg
    31981 taatgatgta gatgcattgc tattctctgt ggacaaaaca ttgaaagaat tagaaggcaa
    32041 agtggacgaa gaagaagtga aaaaagcaga aacagctcgc gatgaattga aagccgctgt
    32101 tgaagcaaac aacatcgaag aaatgaaaac taaacgcgat gcattgaacg aaatcgttca
    32161 aaacttgact gttaaactat acgaacaagc ggcacaacaa caagctcaag aaaacccaga
    32221 agcagctcaa ggcggagcag atgatgtcgt tgatgctgat tttgaagaag tcgatggcga
    32281 cgataaataa tcagttcact gaatttcgag agaaggccaa agttgctact ttggcttttt
    32341 cttttgaaaa atagtttgaa gtatcggttt ttgtttcaag caaaatatga tatgataaca
    32401 agcgacgtga aaatgatgag aaccaatagg aggaaagcct atggcaacaa aacgtgatta
    32461 ttatgaagtc ttagggctct caaaaggagc atcagaagat gaaataaaga aagcttatcg
    32521 taagctttcg aaaaaatacc atcctgacat caataaagaa cctgatgcgg aagaaaaatt
    32581 caaagaagta tcagaagcat atgagatttt aagtgacccg caaaaacgcg ctgcatatga
    32641 ccaatacggg catgcaggca cagatccaaa ttacggtgct ggtggagctg gtggcggatt
    32701 tggtggattc ggcggcttct ctggtggcgg cttcggtggt tttgaagata tcttcgactc
    32761 tttctttgga ggaggcggtc ggtcagtcga tccaaatgcg cctcgtcaag gtgctgattt
    32821 gcaatatacg gtcgacttgt cttttgaaga agcgattttt ggtgtagaaa aagaaatcaa
    32881 gtataatcgg gaagaaatct gtcatacttg tggtgggaac ggtgcaaaac caggcacaca
    32941 accaactact tgtcataaat gtcatggtgc aggtacgatt aatgtagaac gccaaacacc
    33001 acttggacga gtgatgagcc gtcaaacttg tgatgtttgt catgggacag ggaaagaaat
    33061 caaagaacct tgtccaacct gtcatggctc aggacatgag aagaaagcac attcagttaa
    33121 agtgaatgta ccagctggtg tagaagaagg acaacaaatg cgtttagctg gtcaaggaga
    33181 agctggagaa aacggcggac catttggtga tttgtatgtg gtcttccgtg tagaagaaag
    33241 tgatatcttt gatcgtgaag gttctgaaat ttactatgaa ttgcctttaa gttttgtaca
    33301 agcagctttg ggggatgaag tccaagtacc aactgtccat ggagatgtga aattgaagat
    33361 ccctgctgga acacagacag gtacgaactt ccgtttacgt ggaaaaggcg ctccgaagtt
    33421 acgtggtggt gcaacaggcg accaacatgt caaagtaaaa ctgatcacac cgaaaaatct
    33481 aaatgaacaa caaagagaag cattacgtgc attcgctgaa gctggcggca tcaaagttga
    33541 agaacagcaa gaagacggtt tcttcgataa gtttaaagat gcttttggcg ggaaaaagcg
    33601 gaagtagttt ttctagtaaa aacgctaagt cattaataat taaaaaccag acgaacaatc
    33661 atatgttcgc ctggtttttt tgtagttgtc atgtttgtac caacaaaaa
//
LOCUS       DAFBQH010000025        32746 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.025, whole genome shotgun sequence.
ACCESSION   DAFBQH010000025 DAFBQH010000000
VERSION     DAFBQH010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.025"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            131..505
                     /locus_tag="LK694_001245"
     CDS             131..505
                     /locus_tag="LK694_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBK5052126.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="LK694_001246"
     CDS             complement(619..1248)
                     /locus_tag="LK694_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBK5052127.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLPLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="LK694_001247"
     tRNA            complement(1382..1454)
                     /locus_tag="LK694_001247"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="LK694_001248"
     tRNA            1668..1741
                     /locus_tag="LK694_001248"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="LK694_001249"
     CDS             complement(1879..2043)
                     /locus_tag="LK694_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052128.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="LK694_001250"
     CDS             2338..3666
                     /locus_tag="LK694_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBK5052129.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="LK694_001251"
     CDS             3868..4701
                     /locus_tag="LK694_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBK5052130.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="LK694_001252"
     CDS             4717..5454
                     /locus_tag="LK694_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBK5052131.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="LK694_001253"
     CDS             complement(5609..6577)
                     /locus_tag="LK694_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBK5052132.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="LK694_001254"
     CDS             6809..7642
                     /locus_tag="LK694_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBK5052133.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="LK694_001255"
     CDS             7676..8515
                     /locus_tag="LK694_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK5052134.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="LK694_001256"
     CDS             8541..9101
                     /locus_tag="LK694_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBK5052135.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="LK694_001257"
     CDS             9300..11021
                     /locus_tag="LK694_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBK5052136.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="LK694_001258"
     CDS             11186..12328
                     /locus_tag="LK694_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBK5052137.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="LK694_001259"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="LK694_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBK5052138.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..14775
                     /locus_tag="LK694_001260"
     CDS             14128..14775
                     /locus_tag="LK694_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBK5052139.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    14904..15120
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15168..17813
                     /locus_tag="LK694_001261"
     CDS             15168..17813
                     /locus_tag="LK694_001261"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBK5052140.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18123..19436
                     /locus_tag="LK694_001262"
     CDS             18123..19436
                     /locus_tag="LK694_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBK5052141.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19426..20079
                     /locus_tag="LK694_001263"
     CDS             19426..20079
                     /locus_tag="LK694_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK5052142.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20134..20805
                     /gene="radC"
                     /locus_tag="LK694_001264"
     CDS             20134..20805
                     /gene="radC"
                     /locus_tag="LK694_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBK5052143.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(20700..22052)
                     /locus_tag="LK694_001265"
     CDS             complement(20700..22052)
                     /locus_tag="LK694_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5052144.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
     gene            complement(22118..23407)
                     /locus_tag="LK694_001266"
     CDS             complement(22118..23407)
                     /locus_tag="LK694_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBK5052145.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(23494..24198)
                     /locus_tag="LK694_001267"
     CDS             complement(23494..24198)
                     /locus_tag="LK694_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HBK5052146.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            24398..24664
                     /locus_tag="LK694_001268"
     CDS             24398..24664
                     /locus_tag="LK694_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5052147.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            24677..24835
                     /locus_tag="LK694_001269"
     CDS             24677..24835
                     /locus_tag="LK694_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052148.1"
                     /translation="MEEKNIKVPTLEEYTKIVVETDEQNPKTIAVITANDIESCEGFR
                     VRMTPRYD"
     gene            complement(24832..25062)
                     /locus_tag="LK694_001270"
     CDS             complement(24832..25062)
                     /locus_tag="LK694_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBK5052149.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            25134..25289
                     /locus_tag="LK694_001271"
     CDS             25134..25289
                     /locus_tag="LK694_001271"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052150.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            25446..25601
                     /locus_tag="LK694_001272"
     CDS             25446..25601
                     /locus_tag="LK694_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052151.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGEKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(25598..25822)
                     /locus_tag="LK694_001273"
     CDS             complement(25598..25822)
                     /locus_tag="LK694_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBK5052152.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            26089..26424
                     /locus_tag="LK694_001274"
     CDS             26089..26424
                     /locus_tag="LK694_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052153.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            26657..27598
                     /locus_tag="LK694_001275"
     CDS             26657..27598
                     /locus_tag="LK694_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBK5052154.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            27600..28490
                     /locus_tag="LK694_001276"
     CDS             27600..28490
                     /locus_tag="LK694_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBK5052155.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            28533..29333
                     /locus_tag="LK694_001277"
     CDS             28533..29333
                     /locus_tag="LK694_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBK5052156.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            29348..30199
                     /locus_tag="LK694_001278"
     CDS             29348..30199
                     /locus_tag="LK694_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK5052157.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGSIA"
     gene            30196..30555
                     /locus_tag="LK694_001279"
     CDS             30196..30555
                     /locus_tag="LK694_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBK5052158.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            30570..30731
                     /locus_tag="LK694_001280"
     CDS             30570..30731
                     /locus_tag="LK694_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBK5052159.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            30728..31054
                     /locus_tag="LK694_001281"
     CDS             30728..31054
                     /locus_tag="LK694_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052160.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            31051..31866
                     /locus_tag="LK694_001282"
     CDS             31051..31866
                     /locus_tag="LK694_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HBK5052161.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            32005..32316
                     /locus_tag="LK694_001283"
     CDS             32005..32316
                     /locus_tag="LK694_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBK5052162.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            32313..32543
                     /locus_tag="LK694_001284"
     CDS             32313..32543
                     /locus_tag="LK694_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052163.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDNTFRLGS"
     gene            32550..>32746
                     /locus_tag="LK694_001285"
     CDS             32550..>32746
                     /locus_tag="LK694_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBK5052164.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEE"
BASE COUNT        11100 a         5419 c         6654 g         9573 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt aggggaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gggactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcaga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
    14341 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
    14401 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
    14461 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
    14521 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
    14581 atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat
    14641 gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt
    14701 caatacattg atttgacaac cgagttgcag acgcttattt actttgatga gaattactca
    14761 gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat
    14821 tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa
    14881 aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc
    14941 attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt
    15001 gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag
    15061 aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa
    15121 tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa
    15181 ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt
    15241 tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa
    15301 tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc
    15361 aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta
    15421 tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga
    15481 tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag
    15541 agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta
    15601 gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct
    15661 tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac
    15721 gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata
    15781 tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa
    15841 cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca
    15901 tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat
    15961 atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat gatccaaacg
    16021 actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta
    16081 cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa
    16141 tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg
    16201 tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg
    16261 taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg
    16321 aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt
    16381 gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa
    16441 caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc
    16501 ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc
    16561 cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt
    16621 atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg
    16681 aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga
    16741 tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag
    16801 atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct
    16861 atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg
    16921 ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag
    16981 tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat
    17041 ttgatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt
    17101 tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa
    17161 aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc
    17221 caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc
    17281 tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag
    17341 ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata
    17401 caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat
    17461 ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat
    17521 caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct
    17581 tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac
    17641 tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg
    17701 tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag
    17761 agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga
    17821 aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca
    17881 ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg
    17941 tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta
    18001 tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc
    18061 atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt
    18121 tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc
    18181 ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa
    18241 aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc
    18301 tattagaaga ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca
    18361 atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa
    18421 aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg
    18481 aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag
    18541 cgattatcga agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc
    18601 tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag
    18661 aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata
    18721 ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt
    18781 atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag
    18841 tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc
    18901 aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg
    18961 aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag
    19021 gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc
    19081 atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca
    19141 ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag
    19201 taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg
    19261 aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag
    19321 cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt
    19381 tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca
    19441 gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca
    19501 caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta
    19561 ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa
    19621 acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa
    19681 ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag
    19741 attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag
    19801 aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa
    19861 ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca
    19921 ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta
    19981 gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt
    20041 gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc
    20101 ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta
    20161 cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat
    20221 caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt
    20281 gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat
    20341 gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc
    20401 gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc
    20461 tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt
    20521 tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg
    20581 ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca
    20641 gcaaggatcg ttttagcgca taatcatcct tccggaatat ataagttagt acacataaat
    20701 tatataagga aatttactgt tatattgcca tctggaaata ctattatgtt atcgataatt
    20761 gaacgccata atgcattttt ttcttcttcc gaagattctt tataaatttc atcgaattct
    20821 tttgatagaa taaattcgac attttttata tctattttag ctggctctac ttcaatttca
    20881 tttaattctt tcattaaagc ttctcgatct tttttgtatt cctctaagtc tataacttca
    20941 ttcaagtagg ctttcttcaa tctatctaat ttattctcaa tatttttctt actatttttc
    21001 tttttaataa tctttggttg tttttttgaa tagtttattt gtatttgttc aaatttttgt
    21061 ttagtagctt caaaaatata acgttctaaa tttttttcgt aaaaagcgcg cttatttgtg
    21121 caacgtaatt tattatttct tcgttttaca catctataac aagtcctttc gtatatagat
    21181 tctgtaccgt cttttttctt atatcttgct ttaatagttt gaccagctac tgatccctga
    21241 cagtcagaac attttaataa acctttaaaa atgtaatcac gttttgcatt tgtttttaaa
    21301 tttttaggta aggctttttg aacagcataa aaaagttttt tatcaataat aggaggacag
    21361 taatttttat tatttcttaa ttcaccaatg tattttttgt tcgtcaacat atttttaaca
    21421 ctttgataat ctctaacgat tccatactga gagcctaaat aataaacagt ttttcttaaa
    21481 ctaccactag aaagaaagta gttgaatata tctatcacga taggagcatc atcatttatt
    21541 actaatttct tattttctat agagtagcct aacggagttt tcccgcttac tacttcacct
    21601 tgtgcaattt tatttgccat aacagctttg atgcgttctg aatcaatttg ggcttccaat
    21661 tcagcaaaac tcattacttg tgcaatgaaa gttctgccat acgctgtagt tgtatcataa
    21721 tattgttgag aaacagcatt ccaagaaaca ttgtgttttt ctaaaatttc ttgagtgttt
    21781 aaataatgtc gtagatttct aaaccatcta tctaattttg taaataaaat tagatctatt
    21841 ttattttctt ttacatcttg taatagtttt tgaaattcat cacgatttat tttttgacca
    21901 gaaataccac cgtctacata ttcattaact actttcaaat ccttgtgttg ttcgatatat
    21961 tcatagaggg tttctttttg ttctctaaga ctatctccat gtttggcttg ttgttcagtt
    22021 gatactctca tatataatgc tactcgtttc atgttatctc ctttcgaatg tatgttctta
    22081 tcgttgtaaa agaaaagccc gaaggctaat ctttttacta cacatctgtt aattctaatg
    22141 atcttctgta ttgttcagct tgagctagtt tagtaaaaga tacagttccg ttataatttt
    22201 ctaaaacaac tttttcaatt tcatcaagag atactttaaa gaattctttt cgttgattaa
    22261 ctttattgac ttcgtttttt ctaaaatgcg aatgtagttg attttctaat gttggtgcat
    22321 cttctgaaaa tatcattgca tgaacatcaa attcaaaagg tacagaagcg ctactaagtt
    22381 ccttaattct atccataggt tctaaccgtc tagtcattcc tattttatag acatcttcac
    22441 cgaaagatcc gatattactg atgacataaa catagccagc tctagtatta agttctcttt
    22501 gcaatacatc ttctttgaca gtttctagtt ctcttaattt ctcttccagt tctttaattt
    22561 tatcagcata gagttctttt tcaacatcag cactagcttt ttgcatgtat gccattaact
    22621 tagttatctc tgagttaaac tgtcgttctt ctttatcaat gcgtttcttt tcacgttcta
    22681 tttcacgtct taccttttct tcttcaatca tttgttcttt tattgctttt tgctgttctt
    22741 tttcatcgtt aactttaact atgtatgaat agttgagttc taattcttga agttttaatt
    22801 caagtaattc ttttttgaat tctgcttcat cgtttttaaa aagcttgttt attttatcaa
    22861 aagttcttat aattttttct ctagttgaat ctacgttaga aatagtaagt ttattgagaa
    22921 gtccagttat ttctgattct aatggtataa gcattttctt tttaatatta ttagcttctc
    22981 tattattcaa ataagaagga atattaattg caccgtctaa aataaaatcc ttttctttaa
    23041 tctttaataa agaaagtttg ttttttattt cggatgaaga agtttcatct atattcggaa
    23101 taattactga tttatagata gtgaccagag atgtgtcata tttttgatta atttcatcgt
    23161 ctaaccgctg gctttcagca ttaagaatat caattttgtt ttctagcgtt gacaagtttt
    23221 tattcaattt acttatgttt ttctcttttt cagatataaa cgtttcccta gcatttaatt
    23281 cttgagaata ttcctttacc ttactctcta aggcagaagt tttatctttt aattttttag
    23341 cttcagataa tttgctacta cccataatta ttaaaactat tccaactaaa aacaatagta
    23401 ctcccattca aaacgcctcg cttcttttat aaaataattg tcaaaatcat ataaaccctt
    23461 accgaagttg acggctccgg catgggaatt tttttaaaat acaacttttc cgataattct
    23521 aatctgttct ggcgatacaa ttctatcttc gtataagtcg tttatagaac gtaaaatgat
    23581 tgtttgattt tcgtaatctt tatatatctc tttacatgta actccgtcac cacctaattc
    23641 cacaatagca atttccccat cttctacgtc cggttgttct ttaacgaata cgaattgtcc
    23701 attcctgata acaggttcca ttgaatcgcc tataataggt actgcgaagt cagcagcact
    23761 tggaactctc tcaaattctt cctcttcgac aaccgtatca ccgtaagtaa gttctgttgg
    23821 attggcagcc gaagccgaat tgtgaacagt tgggattttt tcttttttag gaaaatctag
    23881 gacattttct tgtttttgtt cttctaatcg attacttgca aattcatagg tagcaacttt
    23941 attttcgtcg ttaagctgat tatatatagg aaggatatta tatatctgat tagatataga
    24001 taaatcaata ttcatcaaat catctatatc aacattgaat atcttagcta tctcatttaa
    24061 ggtttttaat tttggtgtat attttccttt ttcccattcg cttattgagg atgaactttt
    24121 tcttcctagt ttttcggcta gttcttgttg atcaatatta tacttttctc taagaaactt
    24181 tagattttca gcgaacatag taacacctcc ttgagttaat tgtaacaccg tttcggaaaa
    24241 aatgtaacag gtttcttaga aaatatttta gtgtttttaa agaagtattt acgcaaaata
    24301 gaaatcgatt tcaggaaaac tgaaatatgc acttgacttc ggaaaaaccg aagtatataa
    24361 tttagtcaga aagttaaaga gaggaggcga ataaaatatg gataccgcga agaaagaaaa
    24421 atatacattg agacaattaa gagctttaaa gggttattca aaagaagaac tgtcaaggaa
    24481 aagcaaagtg acatcaagaa caatatttat ttacgaaaac gatgtagata aaatgaggaa
    24541 cggaaagtac gccactctag aaaaaattgc taaggcattg ggtgttagag tttccgatat
    24601 ttttttagac cctgattcgg aaaaaccgaa atcaataaca ggtgaaacaa aacaagtcag
    24661 ctaggaggtg tgaatagtgg aagagaagaa tataaaagta ccgactcttg aagaatatac
    24721 aaaaattgtt gtcgaaacag acgagcagaa cccgaaaaca atcgcagtta ttacggcgaa
    24781 tgatattgaa tcatgcgaag gttttcgagt tcgaatgacc cctagatatg attaatgttc
    24841 tttatcacgt ggaggtatag gatcgtttcc aaaactatct tttgattgta tttgtccatt
    24901 ctttttttgg acaataaact ctgattgctg ttttttagaa atctttttac catactcaac
    24961 agcatctgat ttattatcaa atagttttgt atcttcggaa ttaccttcac cacgtacagc
    25021 ccatttgcca tttctaggag atacccattg atttttaccc ataacaattc acctcgcttt
    25081 caagttaatt ctattaagag gtgaaaagaa aacaaactga tggaggtgag attgtgagta
    25141 acaagccaaa aatcactttg aatggcacac ctaatcttca aagattagca gatgaaatgg
    25201 ctagaatacg ttctagacat tggggagtta ctgtggtggc aaccataatt gaacaaaaag
    25261 aaaagcccac acagccggca agcaagtagt gggcaccata gaacaataat attatatcac
    25321 acactcgcca cttccgaaaa gaggagttgc tgctctcctt tcaagtttgc caaagtccac
    25381 gtttcttcat tttggcaact cctcttcttg gaggtggtga cggcgagaga cgaaggaggg
    25441 atgacatgaa ggaagaatca aaaataggga ttttgaatga gaacaattac acaagattaa
    25501 ttattgaaac agaagaaggg gaaaaagtag cagaagttac attgaccgat gcaactcctg
    25561 ctgacggtta tcgtattcga ttaactccta aatatgatta accttttggt gggtgtggat
    25621 cgtttccgtg actatcacgg ctttgaattt ttccatctct gccatgaata aatagctcag
    25681 atccttgatt tttagaaatc tctctagcga tttttactgc ttctgatttt gtattagtat
    25741 gtgcagtcgc tttagaattt ccagcacctt tgacattcca accaccattg gaattaggta
    25801 caacatgttg attttttccc atagttttca cctccatttc tatagaaata ttttatcaaa
    25861 tagaaatgga gaaattcaat aaacgaaagg agggatcaaa tgacattaca agagcatcaa
    25921 cacttgatgc gaaaactaaa tcaggaatac cacgaggaaa tgaaacctag attgctaggg
    25981 gaacaatggc gtgaacgcca gaagaagtgg ttagaaataa aaaagtgact cagccgtcca
    26041 aagcaatgag tcacaaacca aaaattatac ctaaggagat tttatcacat gaataagaaa
    26101 attgaaaatc ttatcgaaga attgaaacgt gaatgccaaa agcaaggtgt ttctattatt
    26161 tgtacagccc aaaaagaagg ggaacttaaa agtctcgttt atggtgagac aacagaaatt
    26221 ttactttgtc ttgctatgca agaagaacat ttagatgaaa atctcccatt gtcagcacat
    26281 attatgcgta gaattgctgt ggatgcttac gaacaagcga aaaatgaaga agaaaatcaa
    26341 ccttcaaatc atacttttgt tattaataac aaggaagatt tagctgatgt gatgactcgt
    26401 attctcaagg gggaatttca atgatgaatg aaaaaacaat tgatctactc attattttat
    26461 ttttcacgtt agtgattgta gcgaaaagtt atgtaatctt tggcttgtta ttcgcaatta
    26521 ccatgcttgt gctttgcgtg ttagcaagca gaaaagaggt taaacatgaa cgagttagca
    26581 aatcttgaca actatttaac tgatcctgat tatgctaaac caccatatga agcgccaatt
    26641 gatgaggagg atgaagatga gtaaatctac cttagaaatg agccatcaag aatggctcga
    26701 agaccgcaaa agaggtattg gtggctcaga tgttgcaacc gtacttggat taaacaaata
    26761 caaatctcct tatcaattgt ggcttgagaa aacgggtcaa attgaattga aagatttaga
    26821 aagcgaacca gcttattggg gtaatgtttt agaagaagtg gttgctaaag agtttcagga
    26881 acgtacaggc aaaaaagtac gcagaagaaa ccaagtattt gaacatccat tacatccatt
    26941 tttaagagca aatattgatc gggaagtagt gggagaaaat gccattctgg aatgtaaaac
    27001 agccaatcaa tttctcggaa aagagtggga aggtgaagag gtaccgctta gctatctctg
    27061 tcaagttcag cattacatga acgttttaaa caaagactat tgttatatcg ctgttttgat
    27121 cggtggacaa aaattcattt ggaagcggat tgaacgagat caagagctga tcgatacaat
    27181 cactgaacaa ttagtagagt tttgggaaac gaacgttctt ggaggtatcg agcctgtaat
    27241 tgacggaagt ggagcgactg ctgacttctt aaaagaaaaa tatgcagatg tagaagaaaa
    27301 tcaaacagct ctaccatcac gttttgatga acttatcgag caaaaaagag aactcaagcg
    27361 gacgaaaaaa gaaattgaat catctattcg tcaagtagac aacgagatca tcagtgagct
    27421 aggtaaacgt gaagccagta tcggtatcac acaaagaaac attatcagct ggaaacttgt
    27481 tcgtacaaga cggatgaaca cgaagaaact agcagagaaa tatccagatg tcgcaaatga
    27541 tgaagagatt tataacgtta ctgaatcaag aaggctaacc gaaaaggaga tcaaataata
    27601 tggcaacaaa tgaatcgtta aaaaatcaat tggcagaaaa gccacagaaa caagttgcac
    27661 caggacagtt agggcttaaa gctctaatga atacaccaac aatgagaaag aaatttgaag
    27721 aagtacttca tgacaatgct aatgctttta tgtcgaatgt tatgactctt gtatctaatg
    27781 acagttatct tgcagatagt gaaccgatgt ctatcatgag tggtgcgtta actgctgcaa
    27841 cattaaatct tgggctagat aagaatttag gttatgcata tttagttcca ttcaatagta
    27901 aaaacaagca aacaggaaaa tgggaaaaga aagctcaatt catgcttggc tataaaggat
    27961 atatccaatt agcccaacga tcaggtaaat acaaagcatt aaatgtgatc gaagtttacg
    28021 aaggagaact aaaaagctgg aaccgactga cagaagagtt tgagtttgat ccaaatggta
    28081 gaacgtctga tgaagtcatt ggatatgttg gctatttcga gttactgaat ggattcaaaa
    28141 aaactgtcta ttggaccaaa caagaaatcg aagctcatcg gattgctaac aataaagatc
    28201 gcgataagac aaagttaagt ggtgtgtggg catctgatta caatgcaatg gcacgaaaaa
    28261 ctgttttgag aaatcttctt tctaaatggg gaatcttgtc catcgaaatg caagaagcca
    28321 ccacatcgga tgagagagtc caaagggttc aagaagacgg cagcattatt gctgaaacag
    28381 aagttgaaga agatattcct gaaagaaaag aagcagaggt tatatctgaa gaaaatgaag
    28441 atgtacaaac tggattattt gatgcatcta atccgccgtt aaacaaataa tgagggagct
    28501 ttctcccttg cttttctaga ggagaaatac gaatgaacac aggatatgtc aaattacatc
    28561 gaaaagtgat gaattcattc gtttggacca atccttatat gtataaatta tggagcttgt
    28621 gtttgatgaa agcgagtcat gagaatcgca agatactttt taatggaaaa gagatacaag
    28681 tgaacagcgg agaattcgtc acagggcgcg atgctatcac atctgagatg aacgaaggcg
    28741 tgaaacgtga acatcaagtg aacagcggtt ctgtatggag atggctaaaa caatttgaaa
    28801 aaaacggaat gttgaacatc aaatcaacta cgaaatacag cgttatatca ataaaaaact
    28861 ggtctttgta ccaaagcagt gaacaacaaa tgaacatcaa acgcacaaca agtgaacaac
    28921 aagtgcacac aaacaagaat gaaaagaatt ataagaatga aaagaataat aaaacatcat
    28981 cgtcgcaacc acgcaaaaag cgtgtttacg acaccgactc agtttactac attctcgcgg
    29041 aggagttatt caaacagatt tgtcagaatc aggaaatcaa aaagccgaat ctgcaaagtt
    29101 gggcagataa cattcggaaa atgatcgaga tcgacaagcg gaccgaaaac caggtacggg
    29161 gaatgattga atggagtcag cacaatgtgt tttgggcatc gaatatttta tctgctaaaa
    29221 aattgcgaga aaaatacgac acaatggcag cgcaagcaaa tcgtgattat aaaacaaaac
    29281 aaactaaaac gcttgaatac gagaaattta gcacagatga gttgcctatt tgagaggagg
    29341 cgtaggcatg gaaactgttg gggaaatcat ggaaaagctg atacagaaag tgcttgtcca
    29401 acgtggcgaa tgtcctgaat gcggacagcc tttgtatgga tggcgtacga agaaccctga
    29461 tggctcagaa cgttgtaagc caacctgtat gcaatgtggg tataaagcat tgcgagttca
    29521 ggaagactta caaacagaac gaatttacaa cgagagcctg aaagcaagag caatcaattt
    29581 tttcaaaggt ggttctgttg tgcctaatca agcgttgttt gattgcacat tgcagaatta
    29641 tcaaattgtc gatcaagaaa caagacaagc ggttgaagta accaaacgct ttgttaattc
    29701 agtcttgtta ggaaatccaa gtcaccttgt tttaactgga aaacaaggaa caggtaaaag
    29761 tcacctagcg atggcagcgg cttgggaagt cttgaagcga tcaaactacg ataaaaaaat
    29821 cttatttatc gggttacagg aaatgctgga tcaaatcaaa ttttcgtaca acaatcctga
    29881 actcagaaaa acgattgagg gatcgttgat tgcagatatc aaaacagcgg atttagtcat
    29941 cattgatgat atcggttcag aactaggaaa agatgcatca gatagtcgag cgtttggcat
    30001 aaacacgcta aattcgttct tggatgcacg acagaaccta gcaacgatta tcacaacgaa
    30061 cttgcttggt gaagaactga aaaaagctta cggtacgaga acaatatcaa gaatgtttgt
    30121 caactctgat ggatttacga tggtattttc tcaaacagca gacaagcgca taaaaccagt
    30181 gaaaggtagt atcgcatgaa taaataccgt aataaaaaaa ctgttcatcg aggtatcaag
    30241 tttgattcta tcgcggaagc agagtattac gatctagcct tgtggcaagc tgaagcgaat
    30301 ggttggaaag tgaagctcca ggaaaggttt gagctgatgc cgaagtttga actagagggc
    30361 aagaagtatc gcaagatcga gtatattccc gacttcacat tttacaaaag cggcaagctg
    30421 gtcaaagtcg tagatgttaa ggggatgcag acaaaagact ttaagatcaa ggcaaagttg
    30481 ttctgtcatc aatatcaagt gccgttgata ttagccaaaa aatatcggaa tacgttcaag
    30541 gaagagcgtt tttaacgagg tggtccatca tgacaacaga agaagtgatt caaatgcgaa
    30601 ttcgaagcct tcggcgtgag attgacgatc tggaacgaac aaaggcagtg atggtcaatg
    30661 aaacggctag aaaggcaatc gatttgcaca tagagaattt aagaagggaa atccgtagat
    30721 tggaggaatg agcgtggata aggaagcggc ttggcgaaaa ttaatgttgc tgattcaaga
    30781 tgagaactgg caagaagatg aagcagtggt tgctgaagtt cagcgtctag gaaagattgc
    30841 taacggacgt atacgaaaaa agccagacaa aagaaaacag cgcaaaggga aaatcgtcgt
    30901 tgttttacac gaaggcaaaa ttttgatgca aggaacagct agtgagctgt ctgcagaaac
    30961 tggatatacg cgtgggacta ttcgaacgta cgcttggcga aatcacaccg atcgaaaagg
    31021 gcacgaatat aagtatttgg aggaagaaaa atgaacgaaa acaaattaat caaattgggt
    31081 gtagcaggag cagtaatcgt aggtattgga gttatcggag gatttaagtt cttcgaaaaa
    31141 atcgataatg gatatgtggg tgtgcgctat tcaatgaacg gcggtatcaa agatgaagca
    31201 ctgacgcaag gtgtgaaatt tgtagggatt gacaaagtga tccaatatcc aattcgcttg
    31261 caaactatcc aatcaaaaaa tatttcagta tctacaagcg acggcaaaaa gacaacgatt
    31321 gatatcaaat atgactacaa agttgattca actaaagcag caaaaatgta caaggaattt
    31381 gggaatatca cttcggaaga tatcgaaagt ggatggttaa aatctaagct tcaaaaggtc
    31441 gctcgtgaag tttatgcgaa atatagtctg cttgatgtcc tttcaggaga ttcctctaaa
    31501 gttgaagctg aggtattaac gaactttgct aaatcagttg aatctaaagg gtttgaagtc
    31561 gaagacgtaa cacttggtgt tccagatgtc gataaagaaa cacaaaaatc aatcgatgcg
    31621 atcattcgag ctggtcaaga aaatgaaaaa gcgaagctag acgcagaaac tgcaaaaact
    31681 caagctgata gtgaagctta caagaaaaca aaagctgcag aagcagaggc agaatctaat
    31741 cgcaaagtcg ccgaatcagt aacagacaat ttgattcgtt atgaagaagc tcaagctcgc
    31801 aaaaagcatg gatgggtaac agtaaatgga gcagatactg tagttacgga tgaagcaggc
    31861 aaataatatg ggattcttta tggctaaaat tctcttgttc ttaggtttag taggagcagc
    31921 atatctcgtg tatgcgctcc tttcccaaac tgatgacaaa gaagatgaca acaacgatga
    31981 aatgaaattt taggaggaga aataatggac gaactaatca caaaagtaga gcagtgggct
    32041 aaagacaagg gactggatca agcagatcca aaagcacagt ttttgaaagt agctgaggaa
    32101 ttcggggaaa tagcttcggc gatggcaaga agtaatgatg aactatttaa agatagcgta
    32161 ggagacgtta tcgtcacgct gattatcctt tccatgcaaa aagggacaaa cgtacaagag
    32221 tgtttagaaa tggcatacaa cgaaatcaaa ggacgcacag ggaaaatggt agatggtgta
    32281 ttcgtgaagt cgagtgattt ggaggatgtg aaatgaaata cgaaatacca ctaagtgaag
    32341 cgggcattca agcaattatc aatggtcggg aggttaacat agaacttcct gatggtactg
    32401 aattagtcat cagacaaagt tatttgaaag atatggcagc tccagtatta attgatcgtt
    32461 ttaacgtgac tgattctgtg gtagagaacc acttaaaaga atttcgatca agtatagaca
    32521 acactttcag attagggagt tgattgacaa tgaacaccag acaccgcaga gtagcaaaac
    32581 taagaaaaca ggaactaaat gtactaaaga caaagtttga aaaagaatat ggaatttcag
    32641 cagaagaaac atataaagtg gcaagtcagt ttgttgctga tgcaagtgat gctattcgta
    32701 agtttgggat ttcgatatta aatgatgatc gtaaatggga ggaaat
//
LOCUS       DAFBQH010000026        32590 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.026, whole genome shotgun sequence.
ACCESSION   DAFBQH010000026 DAFBQH010000000
VERSION     DAFBQH010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.026"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            170..514
                     /locus_tag="LK694_001286"
     CDS             170..514
                     /locus_tag="LK694_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBK5052165.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="LK694_001287"
                     /pseudo
     CDS             515..934
                     /locus_tag="LK694_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1026..1376)
                     /locus_tag="LK694_001288"
     CDS             complement(1026..1376)
                     /locus_tag="LK694_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052166.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="LK694_001289"
     CDS             complement(1342..2673)
                     /locus_tag="LK694_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBK5052167.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVNN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="LK694_001290"
     CDS             2929..3495
                     /locus_tag="LK694_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK5052168.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3640..3727
                     /locus_tag="LK694_001291"
     tRNA            3640..3727
                     /locus_tag="LK694_001291"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3672..3674,aa:Ser,seq:cga)
     gene            complement(3834..4982)
                     /locus_tag="LK694_001292"
     CDS             complement(3834..4982)
                     /locus_tag="LK694_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK5052169.1"
                     /translation="MATFEQYKKKNGEKLWKFQTYLGVDPLTGKQVRTTRRGFKTKKE
                     AQLALTKLQLEYESNGLNKTKELTFQEVYDLWIVNYEQTVKESSFVKTKEQFANHILP
                     AFGALKINKISIDIAQKFANEKVKKFVLYREFINNASRICDYAIKLGYLQDNPFKKIT
                     VPKRKVSVHEKDTLNFFSKEELEIFLKSVEKKKDIRMYSFFRTLAFTGMRVGELLALT
                     WKDIDFDDNYIKINKTLARGKNRRLYVEQPKTKNSKRDIPVDDETMNTLKKWRLEQRK
                     WLLTLGINTLSKNQLVFSNQKNEYLQLSKPRKWLEVIIKQNNLKRITIHGLRHTHASL
                     LLEAGANIKDVQERLGHSSIQITMDLYIHITDKRKEKTAAQFAKYIGI"
     gene            complement(5080..5520)
                     /locus_tag="LK694_001293"
     CDS             complement(5080..5520)
                     /locus_tag="LK694_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052170.1"
                     /translation="MKFIKFGIIGLLAVTVMGGCGLSTAETKESKNYSETNSTIKEVD
                     SDQTAEFATIAEKHIKEIYSIDNLKMDLKSIKVTQFPDDKNAETGEEYKNVYNGTGDF
                     TWQDKKYNFSLIYSKKDATNYTVLYLYSNLDPNKGVNTPLESDQ"
     gene            complement(5606..5806)
                     /locus_tag="LK694_001294"
     CDS             complement(5606..5806)
                     /locus_tag="LK694_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052171.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRKVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            complement(5827..6663)
                     /locus_tag="LK694_001295"
     CDS             complement(5827..6663)
                     /locus_tag="LK694_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5052172.1"
                     /translation="MARPPLNNFEKQLRKEISENLKKVTRGKTQAQISQMTGIPASTI
                     SGYFSQRSTIKEENVKKIAKAFGVSEIDIDPRFAHNYDFLRLKKDVMDELSSGKKFNE
                     NDFLQLAKKTSALGVSLNQMKTERGNNITAVYHDTHDYNFFDTSVAAGIPSSVEAFDQ
                     DHIEQIAIPDYAMGKYAGYQDVFFTKVNGDSMNNVIPNGSLIAVKKVMSCEELKDGDI
                     VVFSNDNEFSVKRYINDQQNKRIIFRPDSSNISFTDIVVNYDDTKYVQIYGKVVVYIV
                     QL"
     gene            6836..7078
                     /locus_tag="LK694_001296"
     CDS             6836..7078
                     /locus_tag="LK694_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5052173.1"
                     /translation="MKKKISLEAARILTGMTQKEAASKFGVNYQTLASWEQDSNKMKQ
                     KYVQMIPNIYNVLPEEIFFGNKNEFTRYWQGKTVFN"
     gene            7117..7866
                     /locus_tag="LK694_001297"
     CDS             7117..7866
                     /locus_tag="LK694_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HBK5052174.1"
                     /translation="MKELIKVTTNEENEQLVSGRELHEFLEVKERYNDWFPRMREYGF
                     SENVDFISFTEKSVKGGRPQKNHFLKLDMAKEISMLQRTEKGKQARQYFIEVEKEYKQ
                     QLLDTSKLSPELQMFQGIFNAVAKQELETKRLATQMDNITEIVALNTTDWRRDCRNFV
                     NKMAQTQGGYGAYQEIQTAIYEEVDRRAGSSLKTRLTNLRNRMAGEGVSKSKRDKTNK
                     LDVIESDKRLKEIYLSVVKDFAIKYGVWKEK"
     gene            7890..8150
                     /locus_tag="LK694_001298"
     CDS             7890..8150
                     /locus_tag="LK694_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK5052175.1"
                     /translation="MEVILTPENEAALRSYIHEIITDEIAKARRDASVDKRVLKQIEI
                     AKYFGVSTATIRKWEDKGLPFGRIGDQKFYDKEKCRAWVLAQ"
     gene            8199..8396
                     /locus_tag="LK694_001299"
     CDS             8199..8396
                     /locus_tag="LK694_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052176.1"
                     /translation="MDKLNTTIVFSAPIIIYLLSVWGSKQALIGVIVYLVWMFAGLDE
                     AEYQTKKRHERRQAESCPEKY"
     gene            8418..8747
                     /locus_tag="LK694_001300"
     CDS             8418..8747
                     /locus_tag="LK694_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052177.1"
                     /translation="MNEKIQNLIKELAAECANDDLGMSVSVVDEKGEVVLGQAGNDSL
                     VAWSVYRQYEKTKRDLQNNNCNCETHGTLKQLFGIEYEDPEFEEWYQDFLRFAEKMDR
                     KKGGIKF"
     gene            8747..8971
                     /locus_tag="LK694_001301"
     CDS             8747..8971
                     /locus_tag="LK694_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052178.1"
                     /translation="MISVKGLGDEIFEAMMHKAQQDVQDKILTAAKYGQTSCTVRSKG
                     LTPSFLAALESEGISNIQCEDGSVKLFWDF"
     gene            8981..9175
                     /locus_tag="LK694_001302"
     CDS             8981..9175
                     /locus_tag="LK694_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052179.1"
                     /translation="MKDFDSLGARQQPQEEARAVGVDWQGNSLYPGDSCYLTEEGYVP
                     VDDILEYAQQHYPKIELGGI"
     gene            9178..10200
                     /locus_tag="LK694_001303"
     CDS             9178..10200
                     /locus_tag="LK694_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBK5052180.1"
                     /translation="MANDLTQTIQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTEGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLANGKETTTVMTKKQIDHSWAKSKMKGSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDVTPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQDEVTRVADVPGHSEIEQAHLIENEDTDPIQEELLDIPDFGREEGVDDVSEFE
                     DDEYPF"
     gene            10163..10978
                     /locus_tag="LK694_001304"
     CDS             10163..10978
                     /locus_tag="LK694_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HBK5052181.1"
                     /translation="MSQNLKTMSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWSPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSRMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEVFMNIYQGEKEMIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLFQQYGKSFMPIIAAVSKQTPSEARLITI
                     DQDNMDYELVMLKEKIERIVRVKDGEEKPNHCGLCEYCRGNLPITGFTNMDDL"
     gene            10994..11776
                     /locus_tag="LK694_001305"
     CDS             10994..11776
                     /locus_tag="LK694_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052182.1"
                     /translation="MTGGWIKLYRQLLEKPIWQESTPEQKVILITLLSMANHEEREWE
                     WQGEPYKASPGQFVTSLDSIRKKCGKGISLQNVRTALKRFEKYEFLTNQPTNKNRLIT
                     IVNWEVYQSKDNDLTKELTSNQQATNKQLTTNKNEKNEKNDKNNNPRNSRKTREYADD
                     DPNKKLAILLLKLIRKNQNIKEPDLDKWANTIRLTIESDKRTGREVQDMIVWATSNDF
                     WSGVILSPTSLRKHFDKMAIQKNKRTQQNISNDELPETGEDW"
     gene            11776..12624
                     /locus_tag="LK694_001306"
     CDS             11776..12624
                     /locus_tag="LK694_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK5052183.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVSGSTIHAVITGRTGAGKTHLGAAVIKEVMMASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKTDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            12621..12863
                     /locus_tag="LK694_001307"
     CDS             12621..12863
                     /locus_tag="LK694_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052184.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            13006..13461
                     /locus_tag="LK694_001308"
     CDS             13006..13461
                     /locus_tag="LK694_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBK5052185.1"
                     /translation="MATRSKYGNKKHEVDGITFDSKAEARYYMKLKRNGMSFMPLSET
                     YCAMQENVLLQEGYLCNDRKIAPIYYRADFVIYENGQVKKVIDVKGYQDAISMLKMKM
                     FAHRYGFPVTFAKFDSKINKFIEMDCFESARQQRKRQTERRKKKLMEGK"
     gene            13463..14038
                     /locus_tag="LK694_001309"
     CDS             13463..14038
                     /locus_tag="LK694_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052186.1"
                     /translation="MTKQVNFRPELKKVTSKSNGNTEVLLVVSNGSLRGSTENLTEFL
                     GSTVTVVIQPETIEYTVPVNKQTKKPNVEYVVNADGTIDMLKEEQTSLDVGDGVEEVE
                     NVKILVSKETIDEFIKTATTLQLPENVTVNIRDVLIRLAEGDSMSEIAADHELSEIAL
                     IDQIELARHYFAPYADSWSKHKDDIIFPEEQ"
     gene            14035..14223
                     /locus_tag="LK694_001310"
     CDS             14035..14223
                     /locus_tag="LK694_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052187.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMDQ
                     KILDVGLLLLHLAEKNLI"
     gene            14231..14437
                     /locus_tag="LK694_001311"
     CDS             14231..14437
                     /locus_tag="LK694_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052188.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEASQWLLKTYTNNKKLRKKCPDL
                     FLKDDQIMPEPMILTSKEGGKCES"
     gene            14628..14846
                     /locus_tag="LK694_001312"
     CDS             14628..14846
                     /locus_tag="LK694_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052189.1"
                     /translation="MDLYDIESGATVSVESDHLIISFADERQIIIWVVDDMLYPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSK"
     gene            14843..15247
                     /locus_tag="LK694_001313"
     CDS             14843..15247
                     /locus_tag="LK694_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052190.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVEIFEGDVVQWGDTPDWEEEPIRVAVVKINPDIQFDSNVG
                     IFEYGRFIYRDTERFLTVLGNVYENPELLEVE"
     gene            15248..15709
                     /locus_tag="LK694_001314"
     CDS             15248..15709
                     /locus_tag="LK694_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK5052191.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            15735..16187
                     /locus_tag="LK694_001315"
     CDS             15735..16187
                     /locus_tag="LK694_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052192.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            16293..16487
                     /locus_tag="LK694_001316"
     CDS             16293..16487
                     /locus_tag="LK694_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052193.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            16518..16817
                     /locus_tag="LK694_001317"
     CDS             16518..16817
                     /locus_tag="LK694_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052194.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVINNGI"
     gene            16807..16965
                     /locus_tag="LK694_001318"
     CDS             16807..16965
                     /locus_tag="LK694_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052195.1"
                     /translation="MEFKIFEEDTRYKLEEKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVSQ"
     gene            17059..17475
                     /locus_tag="LK694_001319"
     CDS             17059..17475
                     /locus_tag="LK694_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBK5052196.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            17791..17997
                     /locus_tag="LK694_001320"
     CDS             17791..17997
                     /locus_tag="LK694_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052197.1"
                     /translation="MLNKASDQVEQLQETLDEIAIVNKAESENSSFFVKANWNIDLPE
                     GDINNPVAEPIKYNPSKAKLDKVH"
     gene            complement(17951..18199)
                     /locus_tag="LK694_001321"
     CDS             complement(17951..18199)
                     /locus_tag="LK694_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052198.1"
                     /translation="MKITAAITKEQGVTFTVVVVKNGVISSSHREQVRSSIPRNFPRP
                     IILAEQSHGKMKYHGRTDIVKFLSNVPYQALPWKDYTL"
     gene            18610..18681
                     /locus_tag="LK694_001322"
     tRNA            18610..18681
                     /locus_tag="LK694_001322"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18643..18645,aa:Lys,seq:ttt)
     gene            18817..19164
                     /locus_tag="LK694_001323"
     CDS             18817..19164
                     /locus_tag="LK694_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052199.1"
                     /translation="MNDFHEAVLSIEVEPSLATAYKKAIEDDNSRHWIKNEIKDADGN
                     IVISDIKPVWNGNYCNVDITDGVRGHSKLTITLLSRTLPNLQEQVDWYKRMGAKVISA
                     NYKGENQNGNEKN"
     gene            19145..19531
                     /locus_tag="LK694_001324"
     CDS             19145..19531
                     /locus_tag="LK694_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052200.1"
                     /translation="MVMRKIRSSITGTEYWDSEKKKTVVVPKGQEPDFEVTEEKGILI
                     DDGSFMTIKGEPITNSNEVLDSDGNTTNDLDGDEVTNDQSVEEADKLDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDILKFIREAE"
     gene            19528..19908
                     /locus_tag="LK694_001325"
     CDS             19528..19908
                     /locus_tag="LK694_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK5052201.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            20020..20472
                     /locus_tag="LK694_001326"
     CDS             20020..20472
                     /locus_tag="LK694_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBK5052202.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            20469..22196
                     /locus_tag="LK694_001327"
     CDS             20469..22196
                     /locus_tag="LK694_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBK5052203.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            22260..23447
                     /locus_tag="LK694_001328"
     CDS             22260..23447
                     /locus_tag="LK694_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK5052204.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            23419..23988
                     /locus_tag="LK694_001329"
     CDS             23419..23988
                     /locus_tag="LK694_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBK5052205.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            24001..25365
                     /locus_tag="LK694_001330"
     CDS             24001..25365
                     /locus_tag="LK694_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBK5052206.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            25367..25645
                     /locus_tag="LK694_001331"
     CDS             25367..25645
                     /locus_tag="LK694_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBK5052207.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            25626..25952
                     /locus_tag="LK694_001332"
     CDS             25626..25952
                     /locus_tag="LK694_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBK5052208.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            25942..26280
                     /locus_tag="LK694_001333"
     CDS             25942..26280
                     /locus_tag="LK694_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052209.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            26270..26635
                     /locus_tag="LK694_001334"
     CDS             26270..26635
                     /locus_tag="LK694_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBK5052210.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            26642..27268
                     /locus_tag="LK694_001335"
     CDS             26642..27268
                     /locus_tag="LK694_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5052211.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            27268..27732
                     /locus_tag="LK694_001336"
     CDS             27268..27732
                     /locus_tag="LK694_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052212.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            27927..30236
                     /locus_tag="LK694_001337"
     CDS             27927..30236
                     /locus_tag="LK694_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBK5052213.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGGVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            30248..30952
                     /locus_tag="LK694_001338"
     CDS             30248..30952
                     /locus_tag="LK694_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5052214.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            30949..>32590
                     /locus_tag="LK694_001339"
     CDS             30949..>32590
                     /locus_tag="LK694_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5052215.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPT"
BASE COUNT        11441 a         5169 c         6827 g         9153 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gtaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 attgacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcagtcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa
     2761 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
     2821 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta
     3721 ccttccttat ataacatttc ataatagagg cgaaaacatt gatatacagg catttgcttg
     3781 caatgttttc gcccctgttt ttttgtttga atacgtcttt gaatacgttc tgcttaaata
     3841 ccgatatatt ttgcgaattg cgctgctgtt ttttcttttc gtttgtctgt aatgtggata
     3901 tataaatcca tagtgatttg aatagacgag tggcctaaac gttcttgtac gtccttaata
     3961 tttgcaccag cttctaaaag taaactagca tgtgtatgtc taagaccatg aatagtaata
     4021 cgtttaagat tattttgttt gataatcact tctaaccatt tacgaggctt agataattgg
     4081 agatattcgt ttttctggtt agaaaatacc agttgatttt tgcttaacgt attaattccc
     4141 aatgttaaca accattttct ttgttctaat cgccatttct tcaaggtgtt catagtttca
     4201 tcatcgactg gtatatctcg cttagaattt ttggttttag gttgctctac ataaaggcgt
     4261 ctattttttc ctctggcgag agttttattt atcttgatat aattatcgtc aaaatcaata
     4321 tctttccatg tgagagctaa gagctcgcct acgcgcatcc ctgtgaaggc tagtgtccga
     4381 aaaaaagaat acatacgaat atcttttttc ttttctactg atttcaaaaa gatttctagt
     4441 tcttctttac taaaaaagtt taaagtatct ttttcatgaa cagagacctt tcttttcgga
     4501 actgtgattt ttttaaaagg attatcttgt agatatccta atttaatagc ataatcacat
     4561 atacgcgaag cattattgat gaattctcta tacaacacaa atttttttac cttttcatta
     4621 gcgaactttt gagctatatc aatcgatatt ttgttgattt taagagcacc aaatgctggt
     4681 aatatatgat tcgcaaactg ttcttttgtt ttaacgaaag aactttcttt tactgtttgt
     4741 tcataattta cgatccataa atcgtaaact tcttgaaaag ttaattcttt agttttattt
     4801 agaccattac tttcgtattc caattgtaat ttggtcagcg ctaattgagc ttcttttttt
     4861 gttttaaaac ctcttcgtgt agttctcact tgtttgccag tcaagggatc tactcccaaa
     4921 taagtttgaa acttccacaa tttttcaccg tttttctttt tgtattgttc gaatgttgcc
     4981 atttttttcg tcctttcgct cgggtaagtg ttcggactaa aatagcttgc atcacctcct
     5041 tagttatacg cttgctttag gctggtaggc gtttatattt tattgatcgc tttctaatgg
     5101 tgtatttacg cctttattag ggtctaaatt actatataga tataggacag tgtaattagt
     5161 cgcatctttt tttgagtaaa tcaaagaaaa gttgtatttt ttgtcttgcc aagtaaaatc
     5221 tccagtacca ttgtagacat ttttgtactc ttcaccagtt tctgcatttt tatcgtcagg
     5281 aaattgagta actttgatag attttaaatc catcttcaaa ttatctatcg aatatatttc
     5341 tttaatgtgt ttttcggcaa tagttgcaaa ttcagcagtt tgatcagagt ctacttcttt
     5401 gatagtactg tttgtctcac tataattttt tgattccttt gtttcagctg ttgataatcc
     5461 acatcctccc ataactgtca ctgctaataa tccaataatt ccaaatttaa taaatttcat
     5521 tttctactcc ttatctctat ggtattattt tattggagaa tcttagaaat aaggtttcga
     5581 gtccgtgttg ctgcacgggc tttttttatt taaataaatc ccaaaaactg aatgtagttt
     5641 tcttgtatac tttattgtaa gcagcctttt ttgggtcttt aatccagccg ctaccttttt
     5701 tcccataacc aggaataact gcttttttta cttttctttt tgcttttcca gtagttctag
     5761 cgctaatgga tttttttata cttggttttc tcattccaat tttcatttta taccctctca
     5821 aattaattat agttggacaa tatatacaac gactttcccg taaatttgaa cgtatttagt
     5881 atcatcataa ttaaccacaa tatcagtgaa agaaatgttg gaagaatcgg gtctgaaaat
     5941 gatacgttta ttttgttgat catttatata tctttttaca gaaaattcgt tatcgttgct
     6001 aaaaacaact atatccccat ccttcaattc ctcacaactc attacctttt tcacagcaat
     6061 caacgaacca tttgggataa cattattcat agaatcccca tttactttag taaaaaacac
     6121 gtcttgataa cctgcatatt ttcccattgc ataatcaggt atagcaattt gttcaatatg
     6181 atcttgatcg aaagcttcga ctgaagaggg gatgccagca gcgactgaag tgtcaaaaaa
     6241 gttataatca tgtgtatcat gataaacagc agttatgtta ttgcccctct ctgttttcat
     6301 ctgatttaaa ctcaccccta aggcgcttgt cttttttgca agttggagaa agtcattttc
     6361 attaaacttc tttccggaac tcaactcgtc cattacatct tttttcagtc ttaaaaaatc
     6421 gtaattatga gcaaatctag gatcaatatc gatctcggag actccaaatg cttttgcaat
     6481 ttttttgaca ttttcttctt ttattgttga tctttgagag aaataaccag aaatagttga
     6541 agcgggaata cctgtcattt gggagatttg agcttgagtc tttccgcgag tcactttttt
     6601 taggttctca gaaatttctt tacgtagttg tttttcgaag ttatttaatg gtggtctagc
     6661 cataactgtc atacctcctg attcattatt atataaaaga ttgtaacgga taaactcgtt
     6721 atttacaact taatagcaat aaaataactt cgttttttaa ataaaaaaac gagtttacta
     6781 attgacataa cgagtaaact cgtttatagt gtttttatga aaaggaggtg tttaaatgaa
     6841 aaagaaaatt tcattagaag ccgctagaat tttaacaggg atgactcaaa aagaagcggc
     6901 ttctaaattt ggcgtaaact accaaacttt agcaagttgg gaacaagatt caaataaaat
     6961 gaaacaaaaa tacgttcaaa tgattccaaa tatttacaat gttttaccag aagaaatttt
     7021 ttttgggaat aaaaacgagt ttactcgtta ttggcaaggc aaaacagtat tcaattaatt
     7081 aaataactaa taaattcgtc gtgaggagtg taatcaatga aagaactaat caaagtaaca
     7141 acaaatgaag agaatgagca gttagtaagt ggtagagagt tacatgaatt tttggaagta
     7201 aaagagcggt ataatgattg gtttccgaga atgagagagt atggattctc tgaaaacgtt
     7261 gattttataa gttttactga aaaatcagta aaaggtggaa gacctcagaa aaaccatttt
     7321 ttaaagttag atatggcaaa agaaatctca atgctccaac gaacagaaaa aggaaaacaa
     7381 gcgcgtcaat attttattga agtagaaaaa gaatacaaac aacaattact tgatacttca
     7441 aagctaagtc cagaacttca aatgttccaa ggaatattta atgcagtagc taagcaagag
     7501 ttagagacaa aacgtttagc aacacaaatg gataatatta ctgaaattgt ggcactgaat
     7561 acaactgatt ggcgtagaga ttgccgaaac tttgtaaata aaatggcaca aacgcaaggt
     7621 ggctatggtg cttatcaaga aattcaaaca gcgatatatg aagaagttga tcgtagagct
     7681 ggttcctctc tcaaaactag attaaccaat ttacgtaacc gtatggctgg cgaaggtgtt
     7741 tcaaaatcca agcgagataa gacaaataag ttagatgtaa tcgaaagtga taaaaggtta
     7801 aaagaaattt atttatctgt agtcaaggat ttcgctatta agtatggagt ttggaaagaa
     7861 aaataaaatt tgataggagg ttcaggatta tggaagtaat cttaacgcct gaaaatgagg
     7921 cagcacttcg tagctacatt catgaaatca taactgatga aattgcaaaa gcaagaagag
     7981 atgcctcagt tgataaacgt gtattaaagc aaatagagat agcgaaatac ttcggagtat
     8041 caactgcaac tattcgtaag tgggaagata aaggacttcc attcgggcgt ataggcgatc
     8101 aaaaatttta cgacaaagaa aaatgtagag catgggttct agcacaataa aatatcgggt
     8161 aagtgttcgg aaataatgac agcaaagaag gggaatttat ggacaaacta aatacaacaa
     8221 tcgtattcag tgcaccaatc attatttatc tgctgagtgt ctggggaagt aaacaagctt
     8281 tgatcggggt aattgtctac ctcgtttgga tgttcgcagg gttagatgaa gctgaatatc
     8341 aaactaaaaa aagacacgaa cgccggcaag cagaatcgtg tcctgaaaaa tattaattac
     8401 aaggagagtt tatcacaatg aatgaaaaaa tccaaaattt aattaaagag ttagcagctg
     8461 aatgtgcgaa tgatgatcta ggaatgtcag ttagtgtagt cgatgaaaaa ggggaagtcg
     8521 ttcttggaca agctggaaac gatagcttgg ttgcgtggag tgtttaccga cagtatgaga
     8581 aaacaaaacg tgatttgcag aataataatt gcaattgtga aactcatggt actttaaaac
     8641 aattgtttgg tattgaatat gaagatccag agtttgagga atggtatcag gactttctac
     8701 ggtttgccga aaaaatggat cgcaagaaag gtggtatcaa attctgatga tctcagttaa
     8761 agggctaggc gatgaaatat tcgaagcaat gatgcacaaa gcacaacaag atgtacaaga
     8821 caaaatctta actgcagcaa agtatggaca aacaagttgc actgttcgtt caaaagggct
     8881 gacaccatca tttctagcag cattagaaag tgaaggtatt tcaaacattc aatgtgaaga
     8941 cggtagtgtc aaattatttt gggattttta ggaggccgtg atgaaagact ttgattcttt
     9001 aggcgcaaga cagcaaccgc aagaagaagc cagagctgtc ggtgttgatt ggcaaggaaa
     9061 ttctttgtat ccaggagatt cttgctattt aacagaagaa ggctatgtac ccgtagacga
     9121 cattctagag tatgcgcaac aacattatcc aaagattgaa ttaggaggaa tttagaaatg
     9181 gcaaatgatt taacacaaac aatacaacgt tcattagacg agcaggtaat cagtaatctt
     9241 gggcggttac aagaacaagg cttagaaatg ccaccaggat atagtccaca aaatgcgtta
     9301 aaaagtgctt ttttcgaact cactaacaat acagaaggaa atttgttgca gatggctgca
     9361 aacaatcaag aaatgaaaac ctccatttct aatgcacttt tagacatggt cattcaaggg
     9421 ttatctccag caaaaaaaca atgctatttc attaaatacg gaaacaaggt tcagctaatg
     9481 cgttcatatt ttggaacaat ggcggtcctt gatcgtgtaa ctggaggggc agatattacg
     9541 cctgtagtcg ttcgacaagg tgatgagttt gaagttgcaa tggatggacc gaacatggtt
     9601 gtcaaaaagc atgaaacaaa attcgagaat ttagacaatg aaatcattgc agcctatgta
     9661 gttatcaagt tagcgaatgg taaagagacc acaacagtca tgacgaaaaa gcaaatcgat
     9721 catagttggg cgaagtcaaa aatgaaaggt tctggaccac aaaaggaatt tccagaagaa
     9781 atggctaaac ggacagtaat caatcgtgca gctaaaacat tgatcaacac aagcaacgac
     9841 aacgatttat tagttcaagc tgcaaaagat acgttagaaa atgaatttga caatgatcgg
     9901 aaagatgtaa cgccacaaac agaaaaagtg gctactctcg aacaaaaatt cttttcaaac
     9961 aaaaagatta ctgaaccaat acaaaaagaa ccagatccga ttgtgattcc agatgatatc
    10021 caagatgaag taactcgagt tgctgacgta cctggccatt cagaaataga acaagcacat
    10081 ttaatcgaaa atgaggatac cgatccgatt caagaagaat tattagatat tccggacttt
    10141 ggacgtgagg aaggtgtaga cgatgtctca gaatttgaag acgatgagta ccctttctga
    10201 tgaaaattat tactccaatg aagctgactg gcagtacatg tcgacatcac aatataagtc
    10261 ctttttaaaa tgtgaagccg cagccttagc aaagttgaaa ggcgattggt caccgacgtc
    10321 ggatcccaaa gccttactcg tcggaaacta cgtacattct tattttgaat caaaagaagt
    10381 acatgaagcg ttcaaagaag aaaataaatc cagaatgttt tctagtagga agccgtatgg
    10441 attgttgaaa gatttccaaa ttgctgaaca aatgattgaa cgacttaaac aagaagaagt
    10501 gttcatgaac atctatcaag gtgagaaaga aatgattgtg actggggaac tatttggaac
    10561 gacatggaaa ggcaagattg attgcttgaa tgttgaggat gagtattttg ttgatatcaa
    10621 aacaacaaaa gacatgcatg aacggaaatg gaacgaaaac tatggatcaa gagaaacgtt
    10681 cattgtcaat tttggttacg tgcttcaaat ggcgatttat caggaattac ttttccaaca
    10741 atatggaaaa agttttatgc cgatcatcgc cgcagtgtcc aagcagacac caagcgaagc
    10801 aagactgatc acgattgatc aggacaatat ggattatgag ctggttatgt taaaagaaaa
    10861 aattgaaaga attgtcagag tgaaggacgg cgaagagaag ccgaaccatt gcggtttgtg
    10921 cgagtactgt agaggtaatc ttccaattac tggattcact aacatggacg atttgtagag
    10981 tggtggtgat gaaatgaccg gaggatggat aaagctttat cgccaattac tcgagaaacc
    11041 tatttggcaa gaatctacac cagagcaaaa agttatctta attacccttc tttcaatggc
    11101 gaatcatgaa gaaagagagt gggaatggca aggcgaaccg tacaaagcaa gtccaggtca
    11161 attcgttact tctctcgata gtattagaaa aaaatgtggt aaaggtatct cattgcaaaa
    11221 tgttagaact gctctaaaga ggtttgaaaa atatgaattt ttaacaaacc aaccaacaaa
    11281 caaaaatagg cttataacca ttgtcaactg ggaggtttac caatcaaaag acaacgatct
    11341 aacaaaggaa ctcacaagca accaacaagc aactaacaag caactaacaa ctaacaagaa
    11401 tgaaaagaat gaaaagaatg ataagaataa taatcctcgc aactctcgaa aaacacgaga
    11461 gtatgcagat gacgatccaa ataaaaaatt ggccattctt ttattaaaac tcattcgaaa
    11521 aaatcaaaac atcaaagaac ctgatttgga taaatgggcg aatacgattc gtttaacaat
    11581 tgaatctgac aaacgaactg gtagagaagt tcaagacatg attgtttggg ccacaagtaa
    11641 tgatttctgg tcaggcgtaa tcttatctcc aacgagtttg agaaaacatt tcgataaaat
    11701 ggctatccaa aaaaataaaa gaacgcaaca aaatatttct aatgatgagt taccagaaac
    11761 aggtgaggat tggtaatgaa taaaaaacta agtgcaatgg ctgcacctta cggcggatta
    11821 aaaacagcag atcataattg cccgaaatgt ggtgatccat tgtatatctg gaaaacaaaa
    11881 aataaagatg gtactgatcg atgcggtcct acatgtatca ataagatttg tggttaccga
    11941 gaaatggtaa ctaaaaatca aaaagaagct atccaaaaag cgaatgaagc aatgaaaaag
    12001 gacgctatca atcgaatgct taacagttca atgattacag atgatgccat atggaccttc
    12061 gattttgatg gatacaaagt agttgatcag gaaacagcac aaataaaagc gatggctcaa
    12121 gaatgggcta aaaaaatcgt aagtggcagc acgattcacg cggttattac tggtagaaca
    12181 ggagccggaa aaacccatct aggtgctgca gtgatcaaag aagtaatgat ggcatctaat
    12241 tataaaattg cctgttcatt tataagctat cgagaattat tagagcaatt gaaatttgca
    12301 atgaatgatc cagaagcaag aaaagctgtg acaggttcgt tgatggctga aattaaaaag
    12361 acggattttg tagtgattga tgatttaggt gcggagctag gaagaatgga agaaaacaat
    12421 caggcaacgc cttatgatgt tgatgttctc acatcgctca cagaagctcg tctaaacaaa
    12481 gctacgatat tcacaaccaa tttatcatcg aagcaattaa aacacgcata cggcgagcga
    12541 gtgttctctc gtgtaatgaa tgggacaaaa ggaaacatag ccgtattcaa aacaacgaca
    12601 gacaaaagga ggaatccagt ttgacctttg tagtgaaaaa aatgtgctac ttagatagcc
    12661 gagggcgagg agaagccagt gtcgagtttg ctaaacacca cacgactaaa gaagaggctg
    12721 accttgttgc aagtgtttgt ggcggcgaag tagtcgaagt tattaaaccc aaaagacgat
    12781 tttcaaaacc taagacaaag ccagtgaaaa aagagtgttg ttgtccgaaa agtaaccaag
    12841 catggatgag aggtgcaaaa tgacttgttt aaaatgcaac gatgaaacag ttatttggta
    12901 taagacatcg cttggatggt caacttgtga accttgtcca atatgcaatg aaaatggacg
    12961 acgttcgaaa gaacgactcg aaagactaaa gaaggagtat agcaaatggc aacaagaagc
    13021 aaatacggaa acaaaaagca cgaagtagac ggcatcactt ttgattctaa agcagaagct
    13081 cgttattaca tgaagttaaa acgaaacggt atgagtttta tgcctttatc tgaaacctac
    13141 tgtgccatgc aagaaaatgt tctgctgcaa gaagggtatc tatgcaacga tcgtaagatt
    13201 gcaccgattt attaccgagc tgattttgtg atttatgaga atggccaagt gaaaaaagtg
    13261 attgatgtca aaggttatca agatgcgatc tctatgctga aaatgaaaat gtttgctcat
    13321 cgatacggct ttccagtgac atttgctaag ttcgattcaa aaatcaataa attcattgaa
    13381 atggattgtt ttgaatcagc aagacagcag cggaaaagac aaacggaacg aagaaaaaag
    13441 aaattaatgg agggaaaata aaatgacaaa acaggtgaat ttcagaccag aattaaagaa
    13501 agtaacatca aaatcaaatg ggaacacaga agtattacta gttgttagca acggatcatt
    13561 gagaggtagt actgaaaatt taactgaatt tcttggctca acagtgactg tagtaatcca
    13621 gccagaaact atcgaataca cagtgccagt aaataaacaa acgaaaaaac cgaatgtcga
    13681 atacgttgta aatgctgatg gcacaatcga catgcttaaa gaagagcaga cttcacttga
    13741 tgtaggtgat ggagtagaag aagttgaaaa tgtgaaaatc ctagtatcaa aggaaacgat
    13801 tgatgaattt attaaaacag caacgacatt gcaactacca gaaaacgtaa ctgtaaatat
    13861 tcgagacgtt cttatccgtt tagcagaagg cgatagtatg agtgaaattg ctgcagatca
    13921 tgaattatca gagattgctt taatagatca aatcgaatta gctagacatt actttgctcc
    13981 atatgcagat agctggtcca aacataagga cgacatcatt tttccagagg aacaatgaga
    14041 gcaactgatc cagtaattat ccttgaggaa gctaaattta tttggactca cgaagagata
    14101 gagcaagcac gcttgctctt ttctcaagga gttaagccga gcaaagtagc tgaaataatg
    14161 gatcaaaaga ttcttgatgt cggattgctt ttgttacatc tagcagaaaa aaatttgatt
    14221 tgaggtgaaa atgatgattc aacttgcagg catacaaaca gggaaaattt atttttccgg
    14281 agaaagcaaa agtgaagcta gtcaatggtt gcttaaaaca tatacgaaca ataagaagct
    14341 gagaaaaaaa tgtcctgatc tatttttgaa ggatgatcag attatgccag aaccgatgat
    14401 tttgacaagc aaggagggcg gcaaatgcga atcttagaga ttgtttggga agtaattaaa
    14461 tttatttcca atatgtattt tacgttttgg ttagtgtgct gccttctata tggattcaaa
    14521 tggcaagtag ggaatttcat gagcgttaaa ttgccgggga tattgagacg gagtaaaaga
    14581 atttacaagg aggacagcaa atgataaaaa agctcattca attcagcatg gatttatatg
    14641 atatcgaatc aggagcaaca gtatctgtgg agtctgatca tttaatcata agttttgctg
    14701 acgaacgtca aattatcata tgggtagttg acgatatgct atatccagaa attgtacatg
    14761 attttgaaga atcaaaagcg gttgagtttg aaatagtgaa aaaagtaatg gaattgattg
    14821 aaaaatacga ggaggacagc aaatgatacc gaagtttaga gcgtgggata aacgaaagaa
    14881 cgtaatgaga gatgtagccg tcttgcattt tactaaaaac ggcaaaacaa actttattga
    14941 atattggata aatcctaccg aattgaaatc atatcacgtg cgaaacatcg acctcatgca
    15001 atccacagga atgaaagata agaatggtgt ggagatattt gaaggggatg tagtacagtg
    15061 gggagatact cccgattggg aagaggaacc aattagagtt gcagtcgtga agattaaccc
    15121 agatattcaa tttgattcaa acgttggcat atttgaatat ggacgattta tttatcggga
    15181 tacagaaaga tttctcactg ttttaggtaa cgtctacgag aatccagagt tattggaggt
    15241 agagtaattg ccaaaaatat tagatgcatg ttgcggtagt agattgttct ggtttgataa
    15301 gaacaattct gacgtaacat ttatggattg tcgtcagcag tatgaagaat tatcaacagg
    15361 tcatgtgatc aatgttgatc cagatgtggt cgcagacttt cgcgatatgc cttttgaaga
    15421 tgatgtgttc gatatggttg tatttgaccc gccacactta agacatgtcg gcgaaaactc
    15481 atggcttgcc aaaaaatatg gaaaattaga cgaactttgg ccagaagaca tcaggcaagg
    15541 ttttgcagag tgtatgcgag ttttacgacc ttcaggaaca ttaattttta agtggaacga
    15601 ggaacagata ccactttctg atgtgttaga ggctattgga gaacagccat tgtttggtaa
    15661 taaacgaagc aaaacacatt ggcttgtgtt tatgaaaaaa ttcagctaac ggaggaaaca
    15721 aaggaggaaa agtaatgaag attgtattaa ataaatgttt tggtggcttt gggttgagcc
    15781 atgaagcaaa aatggaaatt tttaaaagaa aaaatattga agtattccca tacatcaata
    15841 actttagcta tgactcagat gatgaataca cgagatacac aggccaaaag ctaggttcga
    15901 tggattttat ctactatttc aaaaaagacc cgaaaatcga caaagttaca ggaatttata
    15961 gcgaaattga ccgactgtac ggaattgctg atgattccag tttttcaagt gattcaaaca
    16021 gaggtgataa ggacctcgtt gctgttgtcg aaaaactagg aagtgaggcc agtggaccat
    16081 atgcaagtct taaagtcgtt gatatacccg atggggctga gtgggaaata agtgattatg
    16141 acggcgttga aactgcacat tatggttttc aaacaggtag tatttagtcc actaatgaca
    16201 actaaagagg aggcagaagg atgagtgaag atgaatactt tgaacagtag tctattaacg
    16261 acagagattt ttaactggaa aggggtgtag tcatggacgc atcggaagta gtaaataaaa
    16321 ttaatagtct caaagaggtt ttcggagacg tcgaagtagt gataaacgca caaggctatg
    16381 acaacgaatt ttttaagcag attaccgaca ttagcattca gcaaggaatg gaagacgaag
    16441 acggaaactt tatcgatgaa gtagccattt tagtgacttg cgagtaagta attgacagct
    16501 aaagaggagg aagcggaatg aaactaaaag acggatttta ctccagcggc cacggcatcg
    16561 gcggtttaat gctagatatg ccgacaaaga atcctaaaac acgtaagaaa ccaaaattca
    16621 aagtcggtga catggttcgc tgcgaagcag aaggattcat ctatccattt cgtggatatg
    16681 tagaacacgt ctataatcac tcagcaatca ttcgcattga aaacacgatg gaatgtgata
    16741 agtggttagc gaaaagcaaa gagaatttag cagtggtgag attggtggat atggaggtta
    16801 taaacaatgg aatttaaaat ctttgaagag gacactcgct ataaattaga agaaaaatta
    16861 aatgaatttg caaagaataa tgaaattcag catatatctt taacggcttc taagtccggt
    16921 tatacaactt actatgcagc tgttgtgagc tacgtaagtc aataaaagga actcgataaa
    16981 taaaaaagcc ggatcgctcc gactgttcta ataaattcca caaatttatt atatcacata
    17041 aaggagcggt ttgacttgat gcaattgtta cgagaggtag atttcaaaca gacaagatgt
    17101 aatgcgagag atgtgctgaa gaactttcgg cgtttggagc ggatggcagg tcgctctttg
    17161 atagatatta agtcgccgat tattacggat atgccgaagg caccgaagca cggtaataag
    17221 gcagaagacg cgatcattca gatgatggat atagaagcgg agagagacgc aatactagcg
    17281 gctttgatgg ctcttagtct gattagtcgt cagatactct actacagctt ctgtgacgta
    17341 aacaagcact ctaattatga aatagggcaa ttgatacgag gatacggtga aaagaatgta
    17401 gagaagctga aatctatcgc attgattgaa tttgctgaag catacaaaaa aggcgtatta
    17461 gttcagtatc gttgattttg tagggttttt gtagggatag tgtagggttt ttgagtggtt
    17521 taacgtgata ttatgatagt gtcgaaagat tagtgatagg tctaagacaa aataataata
    17581 aaaggaacat cgttttatta ttgtttcaca attaagcttc gatagacagc agcggaaata
    17641 ttaagaataa ggatgtgaat tttaactcct tctaaattgt tcttattatc tatcatccgt
    17701 tgctgtctat taatttatgt attggaggga aaacgaatgg ataaagaaat caaagtaact
    17761 gtcaaattag atttgactga actaaaagaa ctgctcaaca aggctagtga ccaagtcgaa
    17821 cagttacaag aaactttaga tgaaattgct attgtgaata aagctgaaag tgaaaattct
    17881 agtttttttg taaaagcaaa ttggaacatt gatttaccag aaggggatat caacaatccg
    17941 gtagcagaac ctataaagta taatccttcc aaggcaaagc ttgataaggt acattagaaa
    18001 gaaattttac gatatctgtt ctaccgtgat acttcatttt cccatgcgac tgttcagcta
    18061 agataatagg tcttgggaaa tttcttggga tgctacttct aacttgttct ctatgagaag
    18121 aagagatcac accgtttttc acaacaacaa ctgtaaaagt tacgccctgt tctttggtaa
    18181 tagcagcagt tattttcata tattcaccac ctataattta tttcagcgga ccactcgctg
    18241 ataaataaaa ttatacgctt agtatttatt ttcacaatat taatttgtca ctgtggcgga
    18301 aagggtagac gctatatctg gtaggtatgc ttgaagacca gagagacaag agctaagcgc
    18361 aaccatgcaa ggttcgattc cttgccagtg actttagtgg atatccaagg taaaaggaac
    18421 ggaagtggga atcaggcatg attcggtagg ataactgcga gggcatatta agttgaccac
    18481 ttaagtaaaa taattaggga gatgcaccta gaaatagagt cggtcgctct atagggtagt
    18541 catcaaatta gactttgcta tggttagacc ctaagccagt aaagttgtgg cggtgaaccc
    18601 ttgccgcttt gacgtgaagt atctgtggtg atacgtctga cttttaatca gaaggtgcag
    18661 gttcgactcc tgtcacgtca attagcaacc gagggtggta tgaactcgtg tggtgcgagc
    18721 cctagggagg aacaggataa ccgcctgtgt gtaggttgct attacatatt agatcactct
    18781 ttgagtggtc tttttatttt gaaaggagtt ttatctatga atgattttca tgaagcggta
    18841 cttagcatcg aggtagaacc aagtctggcc actgcatata aaaaagcgat tgaagatgac
    18901 aacagtaggc attggattaa aaatgaaata aaagatgccg atggaaatat tgtgattagc
    18961 gatatcaagc cagtatggaa tggtaattat tgcaatgttg atattactga tggagtacgc
    19021 ggtcattcga aattaactat cacattgctt tctagaacat tgccaaactt acaagaacag
    19081 gttgattggt ataaacgtat gggtgcaaaa gtaatcagtg caaattacaa aggagagaat
    19141 caaaatggta atgagaaaaa ttagatcatc aattactgga acggagtatt gggattcaga
    19201 aaagaaaaag acggttgtgg ttccgaaagg tcaagaacct gattttgaag taacggaaga
    19261 aaaaggaatc ttaattgatg atggaagttt tatgactatc aaaggtgaac caataactaa
    19321 tagcaatgaa gttcttgata gtgatggcaa cactactaat gacttggatg gagacgaagt
    19381 tactaacgat cagtctgtgg aagaagcaga taaactggac aacatgactg caaaagaatt
    19441 gcgtgcatat gctaagaaac atggtattga tattcctggc gctatccgtg caaaaggcga
    19501 catcctaaaa tttatccgtg aagcagaatg aagtattgtc agtttgacgg atgtacgaac
    19561 aagatagcaa agggaatata ttgtactgaa cacaagagat caagcagatc acgcaagaag
    19621 aagcagcaag caaagtctgt ttatcatcat gagaacaaac cattctatcg aacgcaagca
    19681 tggaaagata tgcgtcaatt tatttatgaa agagaaggtg gtcattgtca gcggtgtggt
    19741 cagttcatat ttggaaagag agcacacgtc catcacattg taccaatcaa agacaacgaa
    19801 ctgcttaagc ttgatccaaa caatctcatg ctattatgtt caaaatgtca tccaattgtt
    19861 gaaaacgaaa cggaagacaa aaaagttttt ccttcgtatt tcaattgaag cccccctatt
    19921 cattttcaaa attttttcgc gtggggagat agggtagcgg ggagtcacgc gcatcgttag
    19981 gtcaaatttt tcaaaaaaca aaggggggtg tatacaaaaa tgacgactaa agcgcaacgt
    20041 aaagcgatta ttgatgaaaa agtaagtgct gaaaaagctc gtatcttaga aataatgaat
    20101 ttgtctgatt tgtacaccat cactcttgat ccattaatcg aatcatactt ggatattttt
    20161 gaaatttacc aacacaaata tttattgtgg aaggaaaaag gatttccgga gacgcaaaaa
    20221 ttcacgaata aatcaggagc tactaatcaa tcaaaacatc cattggcaca acaagtagaa
    20281 acttgggcag ataagaagat gaaagctcta gatttattag gattaacaaa caaagcaaaa
    20341 actggtagac taattgctgg aggatcgacc gcaagaaaag atgaagaaat tacacgccca
    20401 gaagtaaagc cagtagatga actagcagcg cacagaaata aatggcgtaa gaaggcaggt
    20461 gctgaaaaat gattgaacct ggtgtaaatt atgctgattt atttgcaaaa gaagtaagaa
    20521 agaaacctaa gaagtatcct aagaccgttc gtttagcaat agatcgctgg tatcagtgga
    20581 aaaaaagaaa agatatttgg tttgatgtag atcgcgcgaa tgaaatgatg gattgggtag
    20641 aatcatttat tgttcacaca aaaggagaaa tggtgggaaa accgtttctt ttagaaccct
    20701 gggaaaaatt tatttattct tggatttatg gttgggttaa agaaaatgaa aaaggccaaa
    20761 ttgtacgcgt tactcgtgag gcttatgtcc agattccaaa aaagaatggt aaaacattga
    20821 ttgctgtcgg ttcattagga tatgcaatgt acggagaagg ggcgttatca gttgattgct
    20881 atgcttgcgc atctgatttt gctcaagcgc aatatgctgc caaacctttt gcagttacaa
    20941 tcttaaacaa tccaatccta cttgaaggaa ctaaaatatt tagggggcca aagggaactg
    21001 tttctagtat tacttatgac tatattcatg aagatatggc ttactcaaat aaatttattg
    21061 ttcaaaccaa gaatattgat aatatcgagg gatccaatcc atattttgta ttgaacgatg
    21121 aattgcataa acaggagaaa atggaacagt atgacaattt taagtctgct cagatatctt
    21181 taccacagcc attgatgttt aatatctcaa ctgctgggaa aggttcgtcg tctgttggaa
    21241 tgcgtgtata tcgcgaagca aaagaagtgt tgaaacgtga tgataatgat tcaaactttg
    21301 ttctaatcta tgagccaaat aaaaattatg attggacgga tagaaaagtc tgggaaatgt
    21361 gcaatcctaa ctggggaata tcagtcgatt tatctgcttt agaatcggca tttaaaacag
    21421 cgcaacggtc agctcattcc aaagctgaat ttctaacgaa acacttggat gtatttgtga
    21481 acggcgcgga taatttcttt gaacaggatc aagtagagcc atgtttagtt accacacaag
    21541 aacttggtga tttaagtggc gaaccatgtt atatcggttt agatttatca cgtacacgag
    21601 atttgacctg tgtatcttta aacttcccaa catgggatga agatggtaaa gcggttctta
    21661 aagtaaagca actttatttt attccgaatg aagatttaga gtttcgagaa aaagaagata
    21721 atgttcctta cagtgatttg gctgaacaag gtttcgttga attttgcgac ggaaaaatga
    21781 ttgatcaaga tcagattttg cagtacatag aggactgcat gaatttatat gatattcagc
    21841 aagtgaatta tgatccagcg atgagtgaca aacttgttga gaaattagaa aacttaggat
    21901 tggaatgcgt tgaagttgct caatatccta aagtattgaa tgctcctttt gacgatgtcg
    21961 aacggttgtt ttatgagaaa cgaattcaat ttgataatcc attattcctt tattgcactt
    22021 tgaacgttgt agcaattact aacatcaatg gacaaaaagc gccaagtaaa cgtcaatcaa
    22081 agaaaaagat tgatgggttc gtggcgtttt tgtgtggtca taaggaaacg atgaatcaaa
    22141 tgacagatat tgattcagat gagttagatg attacctaag ttccatctat agataaatag
    22201 aaaggcggtg aaaaaaattg aaattacgtg atcggttatc gaatgcagtc tattctttta
    22261 tggaaaaacg tggatatatc gaggatattt ttggacacta cacacgttat ggtcaaagat
    22321 atgtgacgga ttcatctatc atggaatctt ctgatgttta tgaattagtt caagacatat
    22381 ctaatcaggt ggcattggcc acaccaattg ttattggtcc cgacggcaac gaagtcaaag
    22441 atcatcactt actaaatatt ttgaagagtc ccaatgatta tttgacagga ttcgaattta
    22501 ccaaattgga aacaaatact ttattgatca atggtgagac attcccatta acggataggg
    22561 atcagcttca tttggcgtac ggtgtaacaa ctaaaatcaa tgaacgactt caagaagaat
    22621 ttgaaatgaa tggtcaaaaa atacctggtc aaatgattcg acacattaag aacatcggaa
    22681 ctgattcatt aaaaggtgct ggaataattg atcttgcaag aaacactcta gaaggcgttc
    22741 tgagcgctga aaaagttttg acggacaaat atactaaagg cggtttactt gcgttcatgc
    22801 ttaaactaga cgcccacatc aatccaaata atagcgccca aacaaaaatt gtcaaagcta
    22861 tattggatca actggaagga acgcaaaatg agagtgatca ttctgttaag atgattcctt
    22921 tgggaaaagg atattccatc gagacattaa aaagtcctgt tgatgacgcg gcaattttga
    22981 attatttagg tgtttacaaa aaagacttag gaaaatttct aggaatcaat gttgatacgt
    23041 atcaatcgct gatgaagaca gatattgaaa aagcgatgat gtatctgcac aacaaagcaa
    23101 tcaaaccaat attgaaaaac aagagcgaac attacaccgc tctttttttt atgcctaatt
    23161 ctggctatag agtggaatgg aaaattaata ttttggattt tgttccttac tcaacaaaaa
    23221 caaatattgg ctacaacatc gttcgtaccg ggattacaag tccagataac gtggcagaaa
    23281 tgcttggttt tcctaaacag aatactccag aaacacaagc tatctatatt tcaaatgact
    23341 tatctaggat tggccagaaa aatgcaacag atgattcctt accaacgaat gatcaagact
    23401 tgaaaggagg tgatggaaat gaagaagaag gaaattcgca cgattgacat caccaatctt
    23461 tcaacacgtt ccgatgaaga aactcatacg aggaccatta gcggctacgc tgctgtattc
    23521 aacagtcaaa cacttttatg ggatgactta agcgaagtga ttgttcctgg tgcattctca
    23581 aaggcaatta gtaattcgga tgttcgttgt ttgttcaatc acgattggtc caatgtgcta
    23641 ggacgtacta aaagcggaac ccttcgcctt gaagaagatg aacgtggttt gaaattcgaa
    23701 gttgatttac cggacacaac ggtagcaagg gacttagtta aatctatgga acgaggagac
    23761 atcaaccaat gtagttttgg ttttgtgccg actgaagaaa cgtgggacta caattctgaa
    23821 ccaatgcttc gaacaatcca tgaagtggaa ttatacgagg tttctattgt gcctttgcca
    23881 gcatacgaag atacagaagc tgcattaaga agtcgtgatg aattagaaaa aaacgtcgaa
    23941 gaaagaaaaa aattaatcaa aaaaattaat caagcgctag aagcgtagga ggaaaaatac
    24001 atggataaag aattattgaa aaaaatgaag gcacgtcgtg agcaacgatt gactgaatta
    24061 cgtgaaaaag ttgaatcagg agaattacgc gaagcagatt tagaagccgt gaaagaagaa
    24121 attgacagtg ttattgatga attgaacgga attaaagacg aattaggcgc agattctgga
    24181 actgatgaaa cagacgacaa tacagatgat caatcgaata gtacggattc agacgaaagc
    24241 cgttctggtg aagacaacga tcaagaagaa gattcagaca gtgaagatag ttcagaaaat
    24301 cgttctggaa tgattactca acagcaacga gatggattac ttggatcaat taagaacgga
    24361 ttggaggcac gtgcaaaaat gaccaagaaa caaaaagatc aacaactacg aaaagcattt
    24421 gctaattttg tagctggaaa tatttctgaa gcagaagctc gagctttagg gattgaagct
    24481 ggcaacggtt cagttactgt cccagaagta attgcatctg aagttattac ttatgctcaa
    24541 gaagaaaatt tacttcgtaa atacggaaca gtggtgcgaa catcaggaga tgtcaaatat
    24601 ccaattcttg tgaagaaagc agatgcgaat gtaaacaaga aagagcgttc aactgatatt
    24661 gctgaaacag ctattcagtt tgatgaaatt ttgcttaatc ctgccgaatt cgatgctttg
    24721 gcaacagtaa ctaaaaaatt attaaaaatg tctggtgttc cagttgaaga tattgttgtg
    24781 gaagaattga aaaaagctta tgtgcgtaaa gaaatcaatt atatgttcaa tggtgatgac
    24841 gctggaaatg aaaatcctgg tgcattagcc aaaaaggctg tagcatttaa aaaaccttta
    24901 gatctaactg cttcaggtgc tgggcaaaaa ttatatgatg cattaatcga atttaaaaat
    24961 acaccagtga cagaagtgat gaaaaaggga cgctttatta ttaatcgagc tgctttgact
    25021 gccattgaaa aaatgaaaac agatgatgga tttcctttgt tgcggccatt cacacaagca
    25081 gaaggtggaa ttggttacca attagttggc tatcctgtgg attggacaga tgcagcagat
    25141 aaaaagggtg aaccagacac gccagtttta tattttggcg atttttctgc atttaaaatt
    25201 caagaagtta ttggtgcctt ggaaattcaa aaacttgttg aaaaattctc tggtaaaaat
    25261 caaattggat tccaaattta caacttgcta gatggtcaat tggtttattc tccatttgaa
    25321 ccggcagtat atcgctacga aattacaaaa ccagttggtg gttaagatgg aagagcaaac
    25381 taaagaattg tctttagagg aaaaattcaa atcacatatt cattttgaag agggcatgga
    25441 tgattctttg ctctcttttt atttaaatat ggcaaaagat tatgtcaaaa cagcaactgg
    25501 tggccaacaa gaatatctta ttttgatggt tgccggcatt gcctatgaat atagagtttc
    25561 agaggatgaa ctcgacaaag ctatgaatgc catgacgcca tttatcgtgc aaggagcgat
    25621 tcaaaatgcc gaagagacag actaataatc tcagatggaa agccgaattg ctagacatca
    25681 aaacaggaac agacggaaac gatcgtccaa ctacaattta cgaatttaag cgtctaatat
    25741 tctatgaaga actcggtgtg acttctcaag aaaaatattt atcacagcaa gccaagacag
    25801 acgttgtcag acgaattaaa gtcagatggg ataaatccat cacagagaaa ttaagtgcgc
    25861 tcaaaattga ttctgtaacg tataacatta ctcgcattta tacgaatccc gatgcaagag
    25921 aaatggagtt gagtttagct tatgtcgatt agctttgatg aattgaaaac agcgctgaaa
    25981 tcaacaaagt tagcagtgtt cagagacaaa gccagattag ggacgatgta tccatacatt
    26041 gtgtactcaa atgtgagtaa cagcaaaaaa atggcatccg gtaaagtcta taaaaaatta
    26101 ccttattacc aagtctcttt ttttacactt ggaacagaac aggatttagt tgttattgaa
    26161 caagcgctgc aaaatgctgg cattccatat tcagatttta caggtataca aggtgatgag
    26221 aacgacgata ccgtaaccaa ctattacaca tatgtgaggt gtgtggaaaa tgccaagtaa
    26281 taagaatggt ttttctgaaa tatcggatta tttaggaaac ctttcaaaag ttgatccgaa
    26341 aaaattatcg ttggaatctt tagaagaagc agctaacttt tatctaaagc agttgcttcc
    26401 taatattcct aagtcgctct taaaaaagaa acatatgcgc gatcaaataa aagtagtagt
    26461 tgaaaacgat caagtgaaag ttcaatttga agagacggcc ttttactggc gctttgccga
    26521 aaatggcaca acaaatcaac gtgcccaaca ttttgcaagt ggtacgtatg aacaaaacaa
    26581 agaaaaaatt gaagaaatta tgactagaaa aatacttgat ttatgggaag ggtgatttta
    26641 attgggaaaa caagatactt tttattttga aggattggac gacattttaa ttgcgatgat
    26701 ggcaacgcca gattctgttg gaactgcacc gacttatagt gaagttttac gattgccaat
    26761 tgccacaaaa atcggtgtca aagggaatgg aacagcatta gaaaaatggg catccagcaa
    26821 aatgtttcgt cgtgtgtcgc gagaaacaaa acatgaactt gcgttggatc acgtgggtat
    26881 tccgatagaa gtgatggatg aaatcaaagg attagttgct aaaagcggag taacgtttag
    26941 caaaaatacc gcacgagaat ttccgtactt cgcctttgga tttattggaa atattgaaaa
    27001 tggcggtaaa aaagcagttt ggtatcctaa tacacaacta tccaatgtga ttgacgaaga
    27061 gtatgccact gcagaggaag aaacaaaaat tgacgatgta actgcaaatc tggtttccat
    27121 cggactgaaa tacaataatg tgatgtatgc aagttttgat tcaaatagag acggagcttc
    27181 tctagaatta tttaataaat tcattgctca acctgtatac gatgaagaac agtggaaaac
    27241 attggctaaa gttggaggtg cgggttaatg gctcggttat ctgattatgg aattcacgtt
    27301 gaagacttaa aaaattctgc tgttgtaatt attcaaggcg cagaattccc tatttcattt
    27361 actatgcaaa cgatggaatt tatagcagat gtgtatggtg gagattattc gcaatttgaa
    27421 tctgatatga acgccatgct atctaaaaaa gaaggacaaa tttcttctgc taacttatcg
    27481 cctagtgatc taaaaatcat gcgtgccttg atttatgcca tgctgcgtac tggtggttta
    27541 gaggaagatc cagaaactat tttcaaattt ttgggaatga gtggagaggt gttgtctgct
    27601 tatagtacct gtatggaaat ttttgctagc cagacatttc aggtggaaga cctaaaaaaa
    27661 tccaagaagc cacaagactt tcaaaaagcg caagcaaaaa gaaagaaaaa caaaaagaat
    27721 cggaagagat aggaactcct tggagttttt atatttacgt tgctctcact ctattgaatt
    27781 ggagtgagag tttcttttta aagtctacac ctaacttgtg gctcaaatct tatttacagt
    27841 ggttacaaca aaacaccgat tttgaaccac ctcaatctgt aactatggat aaatcgcctt
    27901 ggtggtagaa aggagcgcta agataaatgg ctggtaaaga atctgatgtc gttcttaatt
    27961 ttaaaacgaa tggcgaagtc agttattcga aaacaatcaa agaaatcaac aaagaaatga
    28021 acttagccgc tgccgagtac aaaaaccaag tgtctgcgat ggacaaggat gcaactcaaa
    28081 cagaaaaatt gcgagcggct aaacaaaaat tagaaaaaca attaggattg gctgaacaac
    28141 gatctcaaat gctgagagag gaatatgaaa aatctgtcaa agaaaccggt gaatattctg
    28201 ctgaatcaga aaaactttat aaacaattac tcaattctga aaccggagaa aataaacttc
    28261 gtacagcatt agaacagacg aacgatgcac ttaaagaaca aggtgacgtt tctgttgata
    28321 cagcaaaaaa actccagaaa atcgaagaaa caggtgagaa agtaaaaggc gttggtgaaa
    28381 aaatgtctgt tggagtaacc gcgcctattg tagcggcagg agcagcagga cttgcagcat
    28441 ttggtgaagt tgacgaggca cttgatacca tcattacaaa aaccggagca acaggtgatc
    28501 aagctgatag actttcacag tctttcaaaa acgttggttc aaatactcat ttacctttac
    28561 aaacggttgg ggaagctatt ggtgaggtaa atacacaatt tggattcatg gataaaaaac
    28621 tggaagattc aaccaattat ctcctacagt acgctgaaat caatgataca gatgtttcgc
    28681 aatcagcaat atctgctcga caagctattg atgcttatgg actagaatat gatgatttga
    28741 attctgtcct tgatgtaaca acgaaaacat cgcagaatac tggtcaatct gtagacgact
    28801 tgatgcaaaa agcaattgat ggcgcaccac aaattaaaca attagggttg agctttggtg
    28861 aaggggtcac tttacttgga caatttgagc aatctggtgt tgattcaagc gcagctttaa
    28921 gtagtttatc taaagcaaca gtggcttatg cgaaagacgg aaaatcttta agtcaaggac
    28981 tagaagaact acaagacaag gtaaaaaatg caggttctga aacagaagcc atcaatgcag
    29041 cagcagaagt attcgggact aaaggcggtc ctagaatggc cgatgcaatt cgtagaggta
    29101 ctttgaacct ggaagattta gcaaaaactg ctggagaaag tgggggagct gtaggagaca
    29161 cattcgatgc tactctcgac ccaattgatc aagccgatca agctatgaac aatgcaaaat
    29221 tagcaatggc tgacgtaggt gaagcagtcc aagtaagcct tttgcctttt tttgaaaaag
    29281 caacttcctt attacaggaa ttctctaaat ggtgggggtc tctagatcaa gatacaaaga
    29341 attggatcat aacaattgct ggtatagcgg cagcagttgg accagtacta atcgttcttg
    29401 gttcgctgat gggatctgta acaaaaatcg ttgggggagt taaaagtttt ataggagttt
    29461 ggcaaggact tgctggttta tttggaatgt ctggtggctg gtttgcatta gcagttatag
    29521 ctattggcgc attagttact ggactaattt gggcatataa taacgttaaa tggtttcatg
    29581 atggagtgaa ttctttcttc aaaggtgttt cagatgtagc tgttgaaata tttaattttt
    29641 taagtggtta ttttggtgga tttttcgatg gtgttatagc aaatttcaat aatttttata
    29701 atgctggaaa acgaattttc agtggttttc tagattttat cactggagta tttacaggga
    29761 attggtcaaa agcatggcaa ggtctagttg atatttttgg aggtattttc gatggtattg
    29821 ttgctgtagg gaaagctcct attaatgcca tgattggatt gatcaatgga tttattggtg
    29881 gattgaataa tataaagata ccaaaatggg ttcctggtgt aggtggaaaa tcattttcta
    29941 tttcaaaact gccttattta gcacaaggcg gtcatctaat caatggacaa gcgattgttg
    30001 gtgaagctgg acctgagtta ctgactgcca agaatggtaa aaccacagtt actccattat
    30061 cagacgaaga aaaacgcaaa gggatcagtg gaagagtttc tggaggtagc attgaacagc
    30121 atatacacat tggcaaagtc gatgcaaaca atccatctga gttagatcgt atgaatcgta
    30181 aatttgctaa agcaaacaaa caggctattc gtgatttggg aggtgttcct atttgagtcg
    30241 acaatttatg aatccagatg aaccaaattt tatttggaaa aatcggaatg cagtaattga
    30301 tatgaattgc atcattgagg cagagcttcc agatattatg ccgaacaagc gatacgaaac
    30361 ttacacgatt cagggccgaa gcggagaatt gacggagacg tttgatgact atgaaccttt
    30421 tgatttggaa atagaaggga tcactattcc acattcgaaa ctgagagaag tcaaacgatg
    30481 gctcactggt aaaagccaat taatcacaca taatgatcca gacaagtatc tagaggctat
    30541 ctgcagcatg gataaagaag tccctttcga aaacgagtgg ggcttctttt atacattcga
    30601 tgttactttt cgttgtcagc cactcaaaag aaaaatcggt gaagcaccta aaatttttca
    30661 ttctagtgtt atggatattt ttgatcatgg tgacgaaatt gcacatccct atctggaaat
    30721 agaatcgaac ggaggagata taacgatcgc tgtcggtgat aaaagcctaa caatacttaa
    30781 tacgttagct gcaacaatcg tggttgatac tcagcttgga aaagcaatac aagaaggttt
    30841 gaatttattt acgcgtggtg attggccagt attgcagcct gaatggaatc aggtaaaaat
    30901 atccggaaaa tttaaagaag tgcggttttg gaataggagt gtgtatctgt gacgcaagaa
    30961 tttatttatg cttataaaaa aatgccagat gatttgagcg tgaacggagc gtcgttagtt
    31021 gattgggaag atttgccaga aatcaatcgt gtgctgaatg gccaatatcg tttttatggt
    31081 aactattcaa gaagcggaca gtatcgctct tatttaaaaa aaggaaattt tataaaagcg
    31141 aaggtaccag atggatcatg gcaatatttt gaaatttata atgtcaaaaa gaatttgacc
    31201 tccgtttctg taacagctcg gcatattggt tttatggcca ataagaactt tattgtcaag
    31261 tcgtttacgg ataacggcaa tgggtctcag atcatgacca atcttaaaaa cagtttggca
    31321 tttgatcaaa aatttaacta tctttcgaat gtagggacaa cgcaccaatt tacagcaaga
    31381 caagtcgcgc cagttgaagc aatcattggt tcaaataacg gtaatcaaaa tttgactggc
    31441 gtgactagcg cggaacttga tatggataac tatgatttga aactggtaaa acagattgga
    31501 tcagataatg gttttcggat tgattttggc atcaatttag aagcaatcga agaagaaatt
    31561 gatgaagaat caattgtcaa tagtttgtat ttggttggcg gtgttcctga taacgattat
    31621 gatgaagaca aagaacctat cgaatatggt tatttagaaa ttgatggagt aaccaacgag
    31681 aatcgacgta ttgccaaacg tgaaaacgga gattgtaaaa cagttgatga attgatcaaa
    31741 tggggcaaaa ccttatttga caatgatcgt atccatgagc caaaagctac tcatacagtc
    31801 agtatggtgg cgttggaaca cacactcgaa tacggtgaca tgtatcaaga gctagcgtct
    31861 ttacactttg gtgatgtcgc tcatgttagg gcgaaagagc tagatatcga gataaaagag
    31921 cgtatggtcg aatacactta tttcccaacg cttgggaaat ataaggatat tgttttaggt
    31981 aatgatttat cactgtacac ttcggcagta aatactcaag cacaagagtt gaaaaagaaa
    32041 atcgacaatc gaacagaaac gttagttcaa aatgtgttga atgcgacggc atggatcact
    32101 ggtaattctg gtgggcatgt cgtttttcgt ccagaaaaag ctccatcaga gattcttatc
    32161 atggatactc caaatgtggc gaatgcaaag cgagtgtggc gttggaattt aaatggctta
    32221 ggttattcag acaacggagt aaatggcccg ttcggtattg ctatcacatc aaaaggagaa
    32281 attgttgctg acttcatcaa agtaggaaca ataaatgcag aagtattcga aacttccttt
    32341 aatgcttatg gtgatgtgtt aaaacttgta aaaggcacgc ttcaaatttg gaatgaaaac
    32401 aaaaaaatca tggagctaac caaaaaaggt atggaattct ggaatagaaa agaatccatt
    32461 ggaacgatcg gaacaacaga ttcagcagga aatccatttc caaatgctgt aacgcctaca
    32521 cctcttgagg aaaattcttt ggtaatacgt acaaatggcg atggaaaata cattttaatt
    32581 tctccaactg
//
LOCUS       DAFBQH010000027        31642 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.027, whole genome shotgun sequence.
ACCESSION   DAFBQH010000027 DAFBQH010000000
VERSION     DAFBQH010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.027"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(238..723)
                     /locus_tag="LK694_001340"
     CDS             complement(238..723)
                     /locus_tag="LK694_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBK5052216.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    813..1158
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1203..1844
                     /locus_tag="LK694_001341"
     CDS             1203..1844
                     /locus_tag="LK694_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK5052217.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1863..2498
                     /locus_tag="LK694_001342"
     CDS             1863..2498
                     /locus_tag="LK694_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK5052218.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2516..3331
                     /locus_tag="LK694_001343"
     CDS             2516..3331
                     /locus_tag="LK694_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK5052219.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3650..5086
                     /locus_tag="LK694_001344"
     CDS             3650..5086
                     /locus_tag="LK694_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK5052220.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5275..7722
                     /locus_tag="LK694_001345"
     CDS             5275..7722
                     /locus_tag="LK694_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBK5052221.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7952..9019
                     /gene="rlmN"
                     /locus_tag="LK694_001346"
     CDS             7952..9019
                     /gene="rlmN"
                     /locus_tag="LK694_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBK5052222.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9212..10315)
                     /locus_tag="LK694_001347"
     CDS             complement(9212..10315)
                     /locus_tag="LK694_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBK5052223.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10868..11416
                     /gene="aac(6')"
                     /locus_tag="LK694_001348"
     CDS             10868..11416
                     /gene="aac(6')"
                     /locus_tag="LK694_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBK5052224.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            11962..13623
                     /locus_tag="LK694_001349"
     CDS             11962..13623
                     /locus_tag="LK694_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK5052225.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(13693..14040)
                     /locus_tag="LK694_001350"
     CDS             complement(13693..14040)
                     /locus_tag="LK694_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBK5052226.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            14124..15065
                     /locus_tag="LK694_001351"
     CDS             14124..15065
                     /locus_tag="LK694_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5052227.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            15066..16109
                     /locus_tag="LK694_001352"
     CDS             15066..16109
                     /locus_tag="LK694_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5052228.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            16115..17185
                     /locus_tag="LK694_001353"
     CDS             16115..17185
                     /locus_tag="LK694_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5052229.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            17175..18128
                     /locus_tag="LK694_001354"
     CDS             17175..18128
                     /locus_tag="LK694_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5052230.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            18140..19150
                     /locus_tag="LK694_001355"
     CDS             18140..19150
                     /locus_tag="LK694_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5052231.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(19435..20256)
                     /locus_tag="LK694_001356"
     CDS             complement(19435..20256)
                     /locus_tag="LK694_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBK5052232.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(20353..20631)
                     /locus_tag="LK694_001357"
     CDS             complement(20353..20631)
                     /locus_tag="LK694_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052233.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            20899..22908
                     /gene="metG"
                     /locus_tag="LK694_001358"
     CDS             20899..22908
                     /gene="metG"
                     /locus_tag="LK694_001358"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBK5052234.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(23387..23974)
                     /locus_tag="LK694_001359"
     CDS             complement(23387..23974)
                     /locus_tag="LK694_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052235.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            24149..25471
                     /locus_tag="LK694_001360"
     CDS             24149..25471
                     /locus_tag="LK694_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5052236.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            25626..26402
                     /locus_tag="LK694_001361"
     CDS             25626..26402
                     /locus_tag="LK694_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBK5052237.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            26395..26964
                     /gene="rnmV"
                     /locus_tag="LK694_001362"
     CDS             26395..26964
                     /gene="rnmV"
                     /locus_tag="LK694_001362"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBK5052238.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            26957..27841
                     /gene="rsmA"
                     /locus_tag="LK694_001363"
     CDS             26957..27841
                     /gene="rsmA"
                     /locus_tag="LK694_001363"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBK5052239.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            28292..28921
                     /locus_tag="LK694_001364"
     CDS             28292..28921
                     /locus_tag="LK694_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052240.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(29045..30154)
                     /gene="ugpC"
                     /locus_tag="LK694_001365"
     CDS             complement(29045..30154)
                     /gene="ugpC"
                     /locus_tag="LK694_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBK5052241.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            30363..30785
                     /gene="mgsA"
                     /locus_tag="LK694_001366"
     CDS             30363..30785
                     /gene="mgsA"
                     /locus_tag="LK694_001366"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBK5052242.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            31108..>31642
                     /locus_tag="LK694_001367"
     CDS             31108..>31642
                     /locus_tag="LK694_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBK5052243.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT        10378 a         5554 c         6587 g         9123 t
ORIGIN      
        1 aatcaaaaga ataaaattcc gatttataat cgaaaaaaga agcattttta tatatgcttc
       61 ttttttcgta gaatcaataa atatggattt ggctaactat ggaagaatac tcgtattagt
      121 tttcagcgtt catactagtt gttacattgt ttgattggcc aaaagaattt ctgatatatt
      181 ttgtgctaaa taaagaaaaa gacgcttgaa ttgggaaata agaaaactac tctatcatta
      241 caaattttca taaactggca gatgttctaa gtaaacaagt atcttttcaa agtcggaaaa
      301 atccataatc aacgtagtgg tattgatatt tggatgaaag cctacggtat cttgacgatc
      361 aatagcagca tcgatcacga cttggatttt tttctctttg tcgtgcatca atgctaatgg
      421 agtcactgta cctgctggaa cgcctaggag gtcaggaagc tgttcttcag aaacaaaaga
      481 taagcgcttt tcttttaatt gttcagctag ttgttttaaa tcggcgtgct tttcatcagg
      541 caagatgaca agatagattt gttttccttt tcttgatttc aataatagat tttttacttg
      601 cggaccaggt agggaaaaag ggacattttt gactgacgta atcgcaggat gatcgacttt
      661 ttgaaaagag atacccagtt tttttagtag ttcataagct tcttgttcgg ttgcacgact
      721 catcgcgcat caccctcctt tttttattag tgtatcataa aaaagagttg acaaatgaaa
      781 aataatcatg taaattacac gtaaagttaa aacaaatgac atcgaaaaga agagtagccg
      841 ttaggtgttt ttagagagtc tctggttggt ggaaagaggc agcagttaac cgtgaaacac
      901 atcttggagt cgattttctg aagcgtatag taggaaaatc cggggagccc gttacagctg
      961 cggtcgttgc tagatgacca gataaggctg gcttttccgt gaggagtcgg taaaaaaagg
     1021 tggaaccacg tgattaacgt cctttgatct tcggatcaaa ggactttttg ttttcatctg
     1081 aacggcaacg actgtgtgag gttttttctg caaggaaaaa ataaacagag gtggaaccac
     1141 gtataatacg tcctcttggc atttgccaag aggatttttt tattttaaag gaggaaaaga
     1201 agatggagta tttgattgaa gttatgccgc aattattagt aggagcactc acaacattga
     1261 aattattttt atttaccttg attggctcat taccattagg gattgtttta gcattaggat
     1321 tgtctttgca ttttaagccg ctacgctact tgcttcaaac atatgtatgg gttatgcggg
     1381 gaacaccgct gttgttgcaa ctgattttta ttttttatgg gttgccgacg attgggatcg
     1441 tttttgatcg tttcgaagca gcactcattg cctttatctt aaactatgca gcttattttg
     1501 cagagatctt tcgtggaggg atacaatcga ttcctcaagg tcaatatgaa gcagcgcaag
     1561 ttttgcaatt aagtcccttt caaacggtga cgaaaatcgt catcccacaa gtgacaaaga
     1621 ttgttttacc ttctgtaggg aatgaagtta tcaacttggt caaagattct tccttgatct
     1681 atatattagg tttaggtgac gtaatgcgtg ctgggaaaat cgcgatggaa cgcgacgcga
     1741 ctttactgcc tttgctaggc gtggcaatca tctatcttac attgactggt attctaacaa
     1801 tcataatgaa acaagttgaa ggaaaattaa attattaccg ctaactgata ggaggaagaa
     1861 atatgattga attgaagaaa gtgaacaaag catttggtga aaaaaaaata atcaaagatt
     1921 tgaatctaat cattccagat gggaaaacat tagctatcgt aggtccttcc ggtggcggta
     1981 aaaccacgct tttacgtatc ttggctggct tagaaagttc tgacagcgga gaattttatt
     2041 tagacggtgt accgttcaac ccattcgagc ttaaaatgaa agaacaagtt gtaggagtcg
     2101 tattccaaga cttccagcta tttccacatc tgaatatttt tgaaaacatt accttagcac
     2161 caaaaatggt attgaaacag acaaaagaag aatacaccaa aaaagcagaa gaattagcgg
     2221 cttcattagg gattagtgat ttattgaata tctatcctta tcagctttct ggcggacaaa
     2281 aacagcgggt agcgattgcc agagcattgg ctatgaatcc acgggtattg gcttatgatg
     2341 aaccaacaag tgctttagat cctgaattac gccagcaagt cgagcgtttg atcctatcat
     2401 tgaaagaaga tggggtaacc caaattattg tcacccatga tttagtattt gcagaaaatg
     2461 tcggtgatca attactaaaa gtttccccaa ttcattaaga aaggagaaga tagagatgaa
     2521 aaaaaccagt tttctattcg ctttgatcgc aggtcttctt ctttttgcag gctgttcgaa
     2581 caaaaaaact tcagcagata attgggcgac tatcaacgaa gagaaaaaaa tcgttatagg
     2641 tttagacgat acatttgttc ctatgggatt tagagataaa gacgggaagc tcacaggatt
     2701 tgatattgat ttagccaaag cagtctttga acaatatggg attacggtag actttcagcc
     2761 cattgactgg tcaatgaagg aatttgaact gaacaatgga acgatcgatt tgatatggaa
     2821 cggctattcc aaaacgccgg caagagaaaa gaaagtgcaa tttacaaaac cttatatgga
     2881 aaacgatcaa gtgctgatca cacctaaaga atcaggaatc acaaactttg gacaaatgaa
     2941 taataagaga ttaggtgcgc aaaatgggtc atctggttat gatgtattta cgaaacaacc
     3001 agaagtatta aaagagatag tcaaaaacca agatgctgtt ttgtatgata gttttaatga
     3061 agcattgatc gatctgaaaa gcagacgggt cgatggtctg ctgatggata aagtatacgc
     3121 agactattat ttgaaacaaa gaaacgaact agaagacttc aacataacaa aaggttctta
     3181 tgagagtgag gattttgcag ttggtgcacg caagtcagat agtacattag tgaaaaaaat
     3241 caatgaagcc ctagatcaac tagagaaaac aggtgaattc caaaaaatat ccgataaatg
     3301 gtttggcgaa gatgttacgc cgagaaatta acaaataggg gactgtgaca aaagacgtgt
     3361 cacagtccct ttttccgaat aaacagtgtt ctcctgccaa ctccttgtga ctcggagctg
     3421 aatggcaggc tcacaacctc ttggtaaacg gtgttcaaaa gccagttttt cggtattaag
     3481 ccaaatttat gcaaaaatgt agaaagccat tttcacaaaa tttttcttac tactcgaaac
     3541 taagcgactt tttcacaacc tcctttacat aattttattt tgttgtggaa ggggtttcgt
     3601 gttattctaa aagaggaacg attgaaggtt ataggaaagg aagagatgaa tggaacacaa
     3661 agaacttacc tcatttccaa aagattttct gtggggctct gcttctgcag cgtatcaagt
     3721 agaaggagct tggcaagaag atgggaaagg agaatcagtc tgggatcgat ttgtacggat
     3781 acctggaaag acatttaaag gaacgaacgg tgatttggca gtagaccact atcatcgtta
     3841 taaagaagat attgctttaa tgaaagaaca aggtctgaaa gcttaccgct tctctgtagc
     3901 ttggacacgg atttttccta atggacgagg agaagtcaat caagctgggc tcgcttttta
     3961 tgagagattg atcgacgaat tgattgaaaa cgatattgaa cctatgttga ctttatatca
     4021 ttgggatctt ccacaagcat tgcaagatga atataatggt tgggaatcac gtcagatcat
     4081 tgaagatttc actcattatg cagaaacatt atttgaggca ttccgcggga aagtccatta
     4141 ctggattagt ttgaatgaac aaaatatctt cacttcatta ggttatttgt tagcagcaca
     4201 tccgccaggt gtgacagacc cgaaacgaat gtatgaagtt aaccatattg ctaatttagc
     4261 aaatgcatct gtcatcaata aatttcacga gttagaaatg cctggaaaaa tcggaccaag
     4321 ctttgcgtat acgccaaatt atccaattga cagtaatcct gaaaatgttc tggctgctga
     4381 aaatgcagaa gacctgatgg ccaattattg gatggatgtc tatatgtggg gaaaatatcc
     4441 gattgctgcc atgaggttct tagaagaaaa aggatgggca ccaacgattg aagctggcga
     4501 tgcagaattg ctagaatctg ctaaaccaga ttttcttggg atcaattact atcaaacggc
     4561 gacgaatgca ttcaatccat taaacggagt tggtgctgga aaaatgaata cgacaggaaa
     4621 aaaaggttca tcagaagaaa ctggagttcc tggaatgtat aaaaaagttg agaatccatt
     4681 tgtcgaacga acaaattggg actgggagat tgacccagaa ggattaagga tcggactgcg
     4741 tcgtatcaca agccgttacc gcatgccggt actgattacg gaaaatggct taggagagta
     4801 tgacaagttg acagaagata aacaaatcca tgacgactat cgaatcaatt acttacaaag
     4861 ccacgtcaaa gccatcaaag aagcaatttc ggatggagca gaagtcctag gctattgcac
     4921 ctggtcttat acagatttgc ttagctggct gaacggttat caaaagcgat atggatttgt
     4981 atatgttgat caagacgaaa cacaagaagg atctttggaa cgatataaga aagacagtta
     5041 ttactggtac caaaaactga ttgaagaaaa tggagagaac ctttagttca catacttaaa
     5101 acaagaaggt acggctggat atggccgtgc cttttcttga taaaatcatt tagaaattaa
     5161 gaaaaactgt aaaataagct gaaaaatgaa agaaagcgtc agttctttca aacaaaactt
     5221 tagttccctt actttttttt gtataatgaa acttgatgaa atgagggata ataaatggca
     5281 caaaagaaac ggcctactaa aaaaaggaaa aagacgaaaa aaacgaaaaa gcaaaaacag
     5341 cagcaggaac aaatgctgac gattggtgtt gggtttgcct tgattttatt tgctgtcttc
     5401 ggctttttaa aattaggttt tttaggaata ttgattgcaa acggctttcg gatcattgca
     5461 ggaaatactt accaaatcct ctgtctttta ttagccgtat taggattttg gattgtcatt
     5521 aagaatacag agttttcaat tggtaaaaac cgacgctggt ttggcggcat actcttttat
     5581 ttcggtatct tattattgtt acatgcccat ttatttggca aactccatac tggggaacca
     5641 aatatcatgg ggacgacatg ggatttgtta gccagtgata taaaacaaag tcaagtagac
     5701 aataacgttg gtggtgggat gattggagca atcctttatc attttactta cttcttgatt
     5761 gcacagcctg gaagttattt agcagcagtg ctattactgg caggaggagc attcctgttc
     5821 agtaatctgg aaggctatca gctgttaaat ggtatccaat caattggaga acgagtacag
     5881 gaattattgg aaggtgatcc tgccaagcaa gctcgaaagc aagcggcaaa ggaagagaga
     5941 atgaaacagc gcgcagaagc gaaagaagca aggcgcttag ctgcaaaaga agctgcagaa
     6001 aaagaagctg cagaatatga gaagaaaaaa gcggtgagcc agcaaaggga aaatactgct
     6061 gatgaatggc aggaaccaga aaatacagag ccggagcagc taagttttgt tccaatcgac
     6121 agttttcaag aaaatatcca tcctgcgaat ctagagaaac cagtaccaga tacaccaaaa
     6181 cagaccaata cagcagaagg tttttcagat gagttgccag aggatgacgg tacctcatta
     6241 gaatttgaga ttgaagcaga acaggaaaat caagactatg aattaccgac agttgatttg
     6301 cttgattcta taccaactgt ggatcaaagt gatgagtata aaaaaattga aaaaaatatc
     6361 ggcgtattag aacaaacttt ccaaagtttc ggtgtcgatg ccaaggtcgt caaagccagc
     6421 ttaggaccat ccgtcacaaa attcgaagtc cagccggctg taggggtaaa agtaagtaaa
     6481 attgtgaact taacagatga tatcgcgctt gctttagcgg cgaaagatgt ccggatggaa
     6541 gcaccgattc caggtaaatc actgattggt attgaagtac caaatggaaa gatcagtatg
     6601 gtttccttta gagaaatcat cgaagcacaa ccaaaccacc cagataaatt actagaagta
     6661 cctttaggac gtgatgtttc tggacgggta caaacggctg atctttcgaa gatgcctcac
     6721 ttactcgtgg ctggttcgac tggtagtggg aagtcagtag caattaatgg gattatcaca
     6781 agtatattaa tgcgtgctaa gccccatgaa gtaaaactga tgatgatcga tccgaaaatg
     6841 gttgaattga atatgtacaa cggcatacct catctgttga cgccggtcgt gacgaaccct
     6901 agaaaagcag cgcaagcctt acaaaaagta gtacaagaaa tggaagaacg atatgaaaaa
     6961 ttcgcggcaa ccggagtaag aaatatttct ggttacaatg aatttgttca acagaaaaac
     7021 ttggaaaacg gaacgaaaca tccaacctta ccattcatcg tagtgatcgt agatgaatta
     7081 gctgatttga tgatggtagc cagcaatgaa gttgaagatg cgattattcg tttagcgcaa
     7141 atggctcggg cagcggggat tcatatgatc ctagctactc aacgtccaag tgtggatgtt
     7201 atcacaggaa tcatcaaagc aaatgtacct tccagaatgg catttgcggt atcaagcggt
     7261 acagattctc ggacgatcat tgattctaat ggggcagaaa agctcttagg gcgaggagat
     7321 atgctattct tgccaatggg tgagaataaa ccgatccgtg tgcaaggagc attcatttct
     7381 gaccacgaag tagaacgggt cgttcaattt gttacagatc agcaagaagc tcattatgaa
     7441 gagaaaatga tgccgacaga tgaagttgaa acgacaggtg ctccagaaca gccacaagat
     7501 gagctgtttg aagaagcaaa agcacttgtc gttgaaatgc aaacggcaag tatttccctt
     7561 ctccaaagaa gatttcggat cggttacaac cgagcagcac gtttagtaga tgaactggaa
     7621 gctcatggag ttgttggccc gtcagaagga agcaagcctc ggaaagtatt cattgagcag
     7681 gaagaaacat cagaaacgtt gccggacgat aatgacatgt aaaacttgtg tgatgaaggg
     7741 ttgaaaaacg aataaccaat aaaaagacaa ggcatgatga taccaaaaga aaaaatggtt
     7801 tttttccatc ttttctaata cggtatgatg tcttgtcttt ttagtttttg tgagattaaa
     7861 gttctattcg atttatttgt gaagtattgc attaaggaaa tgaatcatgt atagtatgta
     7921 gcgaaacttt gagaaaaaga gggaaaatac tttggaaaaa cagtccattt atggcttgac
     7981 gaatgaagaa ttaatcaatt ggtttataga aaacggcgag aaaaaattcc gagctgccca
     8041 agtttgggaa tggttgtatc aaaaacgtgt aagtaatttc acagaaatga cgaatctatc
     8101 gaaacaattg atcgaaaaat tatcggagca ttttatcatc aatcctctaa aacagatggt
     8161 cgtccaagaa gcttcggatg gtacagtaaa atatttgttt gaactccctg ataaaaacat
     8221 gattgagact gtactgatga gacaagaata tggcttgtca gtttgtgtga cgacacaagt
     8281 aggatgtaat atcggctgta cgttttgtgc aagcgggctg ctcaaaaaga accgtgatct
     8341 gactgctgga gaaattgttg cacagatcat gatggtccaa cattattttg atgagcgcaa
     8401 tcttggcgaa cgcgtttcac atgtcgttgt aatggggatc ggtgagccat ttgataatta
     8461 cgacaatgta atggactttt tgcatatcat caatgatgcc aaaggattgg caattggtgc
     8521 tcggcacatt acagtttcca ctagtggatt agcacacaaa atcaaggaat tcgctaataa
     8581 cggcttgcaa gtcaatctgg ctatttcctt gcacgcgccg aataatgagg tgcgaacatc
     8641 gatcatgcgg atcaaccgaa gtttcccaat tgagaaattg atggaagctg tggatgagta
     8701 tttagagaaa acaaaccgtc gaatcacgtt tgaatatatc atgttgaatc aagtaaatga
     8761 tagaccagag catgcgcaac aattagcaga tctattaaaa gataagaaaa aattagcata
     8821 tgtcaacttg attccatata atccagtcag tgaacatgat caatattctc gcagtccaaa
     8881 agctgatgta ttgagatttt atgatgtatt gaagaaaaac ggcgtaaact gtgtgatacg
     8941 taaagaacat ggtacagata ttgatgcagc ttgcggacaa ttgcgaagca agcaaatgaa
     9001 aaaaacagtt gccaaataat cgtatcctac tcgttttttc aattaccata aaaggagcag
     9061 ttttcttaat taaggaactg ttcaacttaa ataagagaaa agattctata gcgaaagcta
     9121 taaagctttt tttgacaaga aaaaccctca tcaagcaaag aaatgtcctc aatttgatga
     9181 cagcctgctt gatgggggtt ttagcaatta tttaactgat tttcttatat ccttgctggc
     9241 tttcttgtgt agcatgataa gtttctacgt caagaatggc ctgtttaaat ttttctcggg
     9301 tgatttcata aggcacatgt ttaatatcat ttgttgccat ggctttatct aagattgctt
     9361 ctaaatcggt cgtgacattg agattcatgt cggttaacga tactggaagt tggttctttt
     9421 tcataaacac atagatttta tcaaacaaat cgaactgctg atccatcatc aataaggtca
     9481 gtacaccata actgacagat gctccgtgaa ggtatccctc cattttttct agaacagtcg
     9541 taccgtaata gaatgagtgt gccatagcag aattatagtt attgttaact aaaacagaaa
     9601 ccaatccggt agtaacaatg atatccgaaa cgacttgttc aattgcatat gagggacgat
     9661 cgtcggcact atctttcaat gcttgcgtac cataagtcaa gagacgatca atgcacattt
     9721 ttccgatttg gattcctaat ctatccgtgt agcctagatc atcgccacga gcagaaaaag
     9781 tagattcgac ttctttggat aaagcatcgc caatacctgc ccataagtat ttatcaggtg
     9841 cttcggcaat aattgctgta tcaatgaagg tgtgtacagg tggttttgaa ggaaagaaga
     9901 gtccgtccat tacgccttct tcgttataga tgacacatac cgcagtgacg ggcgaacagt
     9961 tcgaaccaat agtgggaaag gtgaagactg gtttagctag tttttcacca atgactttta
    10021 cagtatcgca agcacggcca ccaccgactg caaacaggac gtctgctttt tggacacttt
    10081 catgctggat caactgttca atatttccat aagttgaatt acccccgtac cagagactgg
    10141 cgacaacctc tatttcctgt gtcaaagcta cttttatttt gttttctgcc tttgaaagtg
    10201 cagtcttccc tccaatcagc actacttttg tcccatattc tttgacgatt ccatttgctt
    10261 tttcatacgc atctgttcca atggtataat ttggtaagaa aacactttga ctcatattca
    10321 tttccttctt tcttcgttga aacgggttaa aattttaaag agtgatcgat ctgactgcgg
    10381 ctgtagatgg agtagaggtg aacctaagac aggataagtg atgaataagg tggcatgaac
    10441 accttgaaat catcaataag tatcctaaaa acgaacaagt acaaaaacat tgaaaacgac
    10501 aataatgaat ttttgttcct cttagtattt ctaccgattc cctttgcttt tttgtaatta
    10561 tctaaaaatt ctaaaaatta agattattat atgaaaacag gttttatttg tcaattcata
    10621 attggttttt tttaaagcaa aagataatct atatcatgat gaacaaacag agttttttcc
    10681 tccaattgat ggctttcctg atctttccta ttttgaataa agtgtttctg tataattttt
    10741 ttattttata agtaagataa ataatgaaag aatagagata ctgattggta gctaaagatt
    10801 tttgagcgag aacatttcgt tttttcaaaa ggactgttag aataagcaag aacaaggggg
    10861 aagacgtatg ataatcagtg aatttgaccg taataatcca gtattgaaag atcagctttc
    10921 tgatttactg agactgactt ggccggaaga atatggagac agctcggcag aagaagtaga
    10981 agaaatgatg aatccagaac gaatcgcggt agcagcggta gaccaagatg agttagtagg
    11041 atttattggt gcaatccctc aatacggtat cacaggttgg gaattgcatc cattagttgt
    11101 agaaagctcc cgacgaaaga accaaatagg tactcgatta gtcaattact tagaaaaaga
    11161 agtagcttcc agaggaggaa tcacgattta tttaggtacg gatgatttag accatggaac
    11221 aacgttaagt caaacggacc tgtatgagca tacatttgat aaagtggctt ccatccagaa
    11281 ccttcgtgaa catccgtatg aattctatga aaaattaggt tataaaatcg taggtgtctt
    11341 accaaatgca aatggctggg acaaaccgga tatttggatg gcaaaaacga ttattcctcg
    11401 accaaattct caataataaa aagaggttgt gacagaagta atcagcttca agaaataaga
    11461 aggaatttac gaaaattact tcttgaattt ttgtgaaatt ccaacttatt tccgaagaag
    11521 ctacttttgg aacaccgtgt accagaggtt gtgaaaaagt cgcttagttt cgagtagtaa
    11581 gaaaaatttt gtgaaaatgg ctttctacat ttttgcataa atttggctta ataccgagaa
    11641 actggctttt gaacaccgtt tattcagata agggactgtg acaagacttt tgtcacagtt
    11701 tctttttgtt tattcttttt tatctttgta tggcaataat agacaatata acgaaaatta
    11761 tttaatggct tttggaagac catggaaaac cttgtacaaa gctatttttt ctgaaaaatt
    11821 ccttgaaacc tcacgaaaaa atcattattt tatgaaaaag acaaaaaaaa gtgttgattt
    11881 tttaataatt attcattaaa ctcttaaaca acttcagaaa atattaatta ttctgaaaat
    11941 tccaataaag gggtacgtaa aatgaaaaag aaatttacat ttggtttggt tgcggtatgc
    12001 ggccttgtac ttgctggatg ttatggcggc agttcagcca caagcagtag ttcagcaaaa
    12061 agcggttcag cagatggagg aggcgtattt aacttagtcg tgcctcaaga aatgcctaca
    12121 gctgacttat cagttgcaac agatacaatc agttttacag cattgaataa cgtttatgaa
    12181 ggaatttacc gtcttgatga agacagcaaa ccacagccgg caggagctag cgagttggca
    12241 gaagtcagtg aagacggatt aacttacaaa atcaaattac gagaagacgc aaaatggtca
    12301 aatggtgatc ctgttacagc agcggattac gtttatggct ggcaacgaac agtagaccca
    12361 gctacagctt ctgaatatgc ttacctgtat gcgccagttg aaaatgcaga agacattaca
    12421 gctggaaaga aagataaatc agaactaggg atcaaagcag ttggtgacta tgaattagaa
    12481 atcaaattga cgaaacaaac accatatttc caatatctgc ttgctttccc atcatttttc
    12541 ccacaaaaac aatccgtagt ggaagaaaat ggggatgcct atgcatctgc aagtgataaa
    12601 gctgtttata atggaccatt cacattagaa gattttgatg gaccaggaac agatacagaa
    12661 tggacttata agaaaaatga tcattactgg gacaaagata cagtaaaact ttctgaaatc
    12721 aaagtcagtg tagtaaaaga atcttctact gcattgaact tattcaaaga tggccaagca
    12781 gatgacgtga tcttgtctgg tgagttagca cagcaaaacg caaacgatcc agcttatact
    12841 tctgttaaag aagcgcggac aagctatatc gaattcaatc aacgtgaaaa gaattcacca
    12901 ttcaacaatg tgaacctaag aaaagctatt tcttattcta tcgatcgtga agctttagta
    12961 aaacaagtac taggtgacgg atcagttgtc tctacaggat tgatcccatc agatatggta
    13021 aaaagtccaa caacgaacga agactttgca aaagaagctg gaaaattagt ttcttatgac
    13081 aaagataaag caaaagaata ttgggaaaaa gcgaaaaaag aattgggcgt tgattcttta
    13141 gaatttgatt tgatggcttc tgatgatgat tcatcgaaaa aagtaatcga atatgttcaa
    13201 aactcaatcc aagaaaactt ggatggcgta acagtcaaac caacacctgt tccattctct
    13261 gtacgtcttg accgctcaac ttcgggtgat tttgacgcag tactaggcgg atgggctgct
    13321 gactatgcag acccaagcag ctttactgat ttgtttgtca caggtaattc atacaaccgt
    13381 ggtcgctgga gcaatgcaga atatgacgaa gcagttaaag cagcttcatc aacagacgct
    13441 ggcgatgaac aagcccgttg ggatgatttc caaaaagcaa tcaagatcat ctctgatgat
    13501 atgggtgtcg ttccggtgta ccaaaaagca gaaggtcacc tagtgaatga aaaaatcaaa
    13561 ggtatcgttc accatgcggc aggcgcttca tgggattaca aatggacata cgttgaagaa
    13621 taatcgttaa taccgtgagc atgagaaaaa acgaggctgc ggcaaatgtc gcaacctcgt
    13681 tctcatattc acttatccta acaaagaaaa aagcatgaaa atagcagaaa gagcaattag
    13741 tattccggca atgatcagcc aaaagctgta cataccgtag ctggcagacg agagcgagga
    13801 aaattctggt tttttctttc ggttccttgt tcgtgcaaga tggaccgatc gctgaaaatg
    13861 atcgaagaaa cccgaagaaa agacccataa aaaaccgccg ataatcagaa acggcaatga
    13921 ccataagaat agatcattag ataattgaat gagtgtaaag gaatggttga tgagattttt
    13981 tattaaaata accagaatca gtagaaaagc tactgcgtag gggatagatt tttttttcat
    14041 ttgattgctc ctttattttt ggttcattat ctaataatgt agcgaaacat ctgtcagaag
    14101 tcaaaagcta gggggaaaca ttagtgaata gttatatcaa atatgtatta aaacgtgtct
    14161 ttttcatgat catcacgctt tggttaattg caacaatcac ctttttcttg atgcaattgc
    14221 tgccgggcac gccatatacg aatcaggaac gtttgagtcc tgaaacaatc gagatgctaa
    14281 ataaacaagt cggtttggat aaaccagtta tcgtacaata tgggatttat ctttctaatt
    14341 tgcttcaagg agattttgga atttccttcc aatttaaaaa ccagccagtc gcacatttgt
    14401 tagctggaag aatcggacca tccttgcagt tagggttaca ggcaattatc tttggaacag
    14461 tcctagggac gattttagga acgatctctg ccatgaagca aaatacatgg gcagatacat
    14521 cttctacact cgtagcgata ttaggtcgtt ccattccaaa ctttgtattt gctgttttat
    14581 tacaatatat ctttgcaatc aaattacaag ttttgccaat cgcaaaatgg gatggctttg
    14641 tgtacacaat cttaccaaca attgcgttgg ccatgtcgcc gcttgctgac tctgctcggt
    14701 ttatccgtac ggaaatggtg gaagtactgc atagtgatta tgtggaatta gcaaaagcaa
    14761 aaggattgag ccgttgggaa atcgccttta aacatggtct tcgaaatagt ttgattccat
    14821 tgatgacttt attaggaccg ttagctgtag cattgatgac cggatcactg gtggtagaaa
    14881 atatctttgc tattcctggt atcggggaac aattcgttaa gtcaatcaca acaaatgatt
    14941 atcctacgat catggctgta acgatccttt actcatttat gctgattttt gttattttga
    15001 tcgtggatct attgtacggt ctagttgatc caagaattcg tgtttcagaa gggagccgag
    15061 gataaatgga aatgatacca aagcgttatg aaacagttgc agatatccct gcggatgaat
    15121 ttcttccatt acaaaaaaat accgaagaag aacgtgaaca gattactgca ccttctttga
    15181 attttatcca agattcatgg cgccgtttaa agaaaaacaa agctgccgtt atttcaatgg
    15241 ttttactatt ggtcatcatt ttgatctctg tgatcacaat ctttgtttct cctcatgatc
    15301 caactgccca aaacgtggct tatatcaatt tgcctcctcg tattccggga atcaatatcg
    15361 atggattgaa tggaaaagca atggttggtg gtgaacttgt tgataaatat gctcaagcaa
    15421 acgtcccttc aaacgtaaat ttctttttag gaacagacgg tttgggacgt gatgtattaa
    15481 gccgcttatt tatgggaatg agaatctcgt tgctgatcgc atttattgct gcattactag
    15541 atgtaacgat tggtgtagct tacgggttgg tttctggttt gttaggcgga cgtgtcgata
    15601 acgtgatgca acgttttttg gaaatcttat caggaattcc aaacctcgtt gtcatgattt
    15661 tgatgttggt cgtttttgaa ccaggaattt tttcaatcgt ggctgctatg gccatcacaa
    15721 actggatacc aatggcacgt atcgttcgtg cacaaacaat gaaattaaaa gatcaagaat
    15781 atgttttagc tggattgaca ttaggtgaat caagaatgaa aattgcattc aaacatattt
    15841 tgccgaatat ctctagtgtc attattattc aaatgatgtt tagtattcca tctgctattt
    15901 tcttcgaagc ctttttaagc tttatcggtt taggtcttac gcctccgtca gcttcattag
    15961 gaacgatgtt gagcgaaggg tacaagacat tcctgtatct gccttatcta ttatggattc
    16021 cggcagtaac tttatcggtt atcatgattg gctttaactt acttgctgat ggattgcgtg
    16081 atgcctttga tcctaaaatg aaagagtgaa gacgatgacg aaaattttag aagtgaaaga
    16141 cttagaaatc tcttttgata cgtatgctgg aaaggtacga gcgattcgcg gtgtcaattt
    16201 tcatttgaat aaaggagaaa cattggcgat cgttggggaa tctggaagtg gaaaatcagt
    16261 gacgaccaga agcatcatgc gcttgctgtc aagcaacgcc aatatagatg ctggtcaaat
    16321 cctctttaaa ggtaacgata tcgttggaaa atcagaaaga gagatgcaaa aaatccgtgg
    16381 taaagaaata gcgatgattt tccaagatcc aatgacctct ttagatccga cgatgcccat
    16441 tgggaagcaa gtcgctgagt cccttagaaa gcataataaa gtttcaaaaa aagagggaca
    16501 aaaagctgca ttagaattgc ttaaattagt tggaataccc gaagctgaga aacgtatcaa
    16561 cagttatccg catcagttct caggaggtca gcgtcaacgg atcgtgatcg cgattgcact
    16621 ggtatgttat ccggaaatcc tgattgctga tgaacctaca acagcgcttg atgtgactat
    16681 tcaagcacag attttggagc tgctgaaaga aattcagaca aaaatcgata catcaattat
    16741 ctttatcact catgaccttg gtgtagtagc gaatgtcgct gatcgtgtcg ctgtcatgta
    16801 tggaggaaga atcgttgaag ttggaacatc ggaagaaatt ttctacaacc cgcaacatcc
    16861 ctatacttgg ggactgcttg gttcaatgcc gacattagat agtgcgaacg atcgattgta
    16921 tgctattcct ggttctccac cggatttatt gaacccgcca aaaggagacg cattctatcc
    16981 aagaaatgaa ttcgcgatga aaatcgatgc agaacaagaa ccaccatttt ttgaactatc
    17041 aaaaacacac aaggcagcaa cttggctact tgctccgcag gcacccaaag tgacaccgcc
    17101 agaagaaatc caacgtcgct gggctatctt taaagagaaa caaaaacata cttacggtca
    17161 aggaggaatt gctgatgcca aataaactac ttgaagttaa aggattgaaa cagtatttca
    17221 atgtaggaaa aaagaatgaa gtacatgctg taaatgacat cagctttcac atttatgaag
    17281 gagaaacatt cggtttagtt ggagaatctg gaagcggaaa atccactact ggccgtacga
    17341 tcattcgctt gaatgaacct actgatggtg agatattatt cgacggtcaa gatataacga
    17401 agatcaaaga taaaaaaggt ttgacgaaat tccggcatga tgttcagatg atctttcaag
    17461 atccatacgc ttcgttgaat cctcgtatga aagtcagaga tatcattgcc gaaggaattg
    17521 atgttaatgg actagctaaa tcatctgaag agcgtgcaaa aaaagtcgat gatctattga
    17581 gaactgttgg attgaatcca agccacggta ctcgttatcc tcatgaattt tcaggtgggc
    17641 aacgccagcg tattggtatt gcacgagcat tggcagtaaa accgcgtttt atcatttgtg
    17701 atgaaccaat ttctgcacta gatgtgtcga tccaagccca agtggtcaat ttgttgcaag
    17761 atctccaaaa agaacatcaa ttgacctatc tatttattgc acatgatttg tcgatggtta
    17821 aacatattag tgaccgaatc ggtgtaatgc ataacggctt gttattagag atgggaacaa
    17881 gtgatgaaat ctataatcac ggtgttcatc cttatacaga aagtttgtta tctgctattc
    17941 ctctaccaga tccagaccat gagagaaaac gcagaagaat caaatatcaa cctgaaccag
    18001 atgacggaca agtacgtcaa cttcgtgaaa tcgcaccaga acattttgtc tatgcaactg
    18061 agcaggaagt cccatattac gcaaaaaaac taaaacgaca aaaggaatct cttctggttg
    18121 ctaactagga gggacataga tgctaaaagc ttttaaattc cgtatgtatc cgacagaaga
    18181 acaaaaacag caattgattc gtacttttgg atgtgctcga tttacctata accatttgct
    18241 gaaaaagcgg cagaaaagtt ggcaacaaac aggagtagcc aatttttcgc tcacacccgc
    18301 tacattaaaa aaagaatacc cattcttaaa agaagtagac agtcttgctt tagcaaatgc
    18361 acagcttaat ttggatcggg cttttcgcaa ttatttcaag ggacgtgcca gcttcccaaa
    18421 gcttaaaacg aaaaaaagta tgtggcaatc ttatacgacc aacaaccaaa cccgtacgat
    18481 ctatctggaa aatggctact taaagttacc aaaacaaaaa gaactaatca agataaatca
    18541 gcatcggcca gttgaaggat cgattcgctc cgctacgatt tctgctcgat ataatgaaga
    18601 attttatgtc gcactacttt gtgatgtttc gccagtaaaa aaagaatctt tagctaaatg
    18661 gatcgggatc gcctatcatc cgaagacatt gatccaaaca tctcgacctc tagaagtaac
    18721 tttaccaaaa tttcaccaga cagaagaaaa gctgcaacat gcacagcgga aattgaatgt
    18781 gaaagtccgc tccgctcacc atagaaagat caggttagat caggcaagca attatcagaa
    18841 acaaaagaga aaagtaatgg atctttatct gaaacaaaag aaccagcgag aagattatct
    18901 tgaacaatta tctgggaaat tggtcaaaca atatgactac ttatttgtag aatcttttcc
    18961 aaaagaagag gcacatgctg atttttctat tcacgactgg cataaattaa taacaaaatt
    19021 gcgttataag tcccaatggt ataataaaaa atttcttttg atcaataccg acggagcaga
    19081 agaaagcaat tccgtaagaa agagccaagt agtagaaaaa atgggcagac attcggttat
    19141 caagggataa aaaatgaatg aaactgatag gagcgttagg gttagccttg ttaataaaaa
    19201 gtcttaatga cttttgtttc taggaatttt tcttttgaag aaaagctcac taattatgac
    19261 aaaccaataa aacaatagca gaaaaagttc gtacgagctt tttctgctat tgttaatttt
    19321 ttattcagaa agtttacgaa tacatcaaaa ttctgaaaca aaaaacgtaa ctcacaaaga
    19381 gttgtttttg cgttttttct tgaatgatta ggtctgaatc ttttgctcac aaccttattc
    19441 atggtgcccc ggcttgagct gatagatttt tgaaggacgg ccgattccta caggacgttc
    19501 accgatctca tcgaaatatt ggagcatagc tttcttaaaa ttggaatgat cgatcgtttt
    19561 ataatcgatc cctaagaatt tagcaaatac ttttcgagct tccgtaatcg tgaaatcttt
    19621 acctaaaact tgtagaactt gcggctcgtg ttccatttta ttgactacac ggttgaatgc
    19681 tttcaagatg atttgactat ggtcaaaagc caaagtatct ttgccagaag actctccagt
    19741 gactaaatca agcgagatag ccacttctcc gctagataaa tggatcttgt tactatgacg
    19801 ttctagtgtg aaccagcgta cttccttggc atcatctccg gcgattagcg gttcttctcc
    19861 aataaaggct aagtagctga ctgtgacgac ccagcctcgc ggatctcgat taggcgtact
    19921 gaatgtatgc agttgctcaa tattttcttt tgaaataacc acaccagttt cttcctttgt
    19981 ttctcttaag acgctttctc cagttgactc atccttttgg acaaagccac caggcaatgc
    20041 ccatgaattt cgataaggat gcccttttct ttggatcaac aaaactttca gctgatcttc
    20101 ttctttgtta tagcacatca gtacgatatc gacagtcaaa gagggcttgt catattctgg
    20161 acgttcctgt ttttgatacc agttaagaaa ttcttcttca cttgctttat gttcataata
    20221 taatttttct tcttctttag aagcaaaacg gttcatctga ccacctccat tgacagtaaa
    20281 agtacttttt accttattgt agcagactct gaaaaaaaac caaggattta cttgaaaaat
    20341 ctaggttatc atttaaatat gaaaaataaa tggaagtatg agattgaata aaaagaaaag
    20401 cagataaaac ggcaatactt tataccgctc ttcacgaatg gcaaagaagc cacaaataac
    20461 gatatatacg tcaatgataa aacgcaaaat ccaagctgct gtcataaaac tgttcaaatg
    20521 cccatggagg ttaaaataac catacatacg aatataaaaa tagctgaata ttaaatgtgc
    20581 taatgaaaga aaagcaatac taagtgtaaa aaaaagatgt ttcttctgca tgattgttcc
    20641 agtcccttac taagatagat agtctttatt ataccacgtg tgaggggaga aataaaaagc
    20701 cgttttttag tagatatgaa acaaaacaaa aacccgcatt tattcaacct attcatcctt
    20761 ttaaaggata gattgaacgt agatagaagt cgtttatcca gtctttttca ttttgaaaaa
    20821 aagctaacag catgcttttt ttatggtata ctagcaagga aacgaattcg catcatgcga
    20881 taaaggagga agagccccgt ggcagaaaag gaaacttttt atatcactac gccaatctat
    20941 tatccaagcg gcaaacttca tatcggcaac tcgtatacta cgattgcatg tgacgcaatt
    21001 gctcgttata aacggttgat gggctttgat gtgttttatc tcactggagt ggatgaacat
    21061 ggtcaaaaaa tcgaaacaaa agcagaagaa ctaggtgtaa aaccgcaaga atatgtagat
    21121 aaaatggcgg ctgatgtcaa gaaattatgg aaaacgcttg atatcagtta tgataaattc
    21181 attcgcacga cagatgatta ccataaagca gctgttcaaa aaatatttga tcgtttatta
    21241 gaacagggag atatttactt aggtgaatat gaaggctggt actctgtttc tgacgaagaa
    21301 ttctttacag aaacacaact tgcagaagtg tacaaagatg acgaaggtaa cgtgatcggt
    21361 ggtaaagcgc caagtggaca cgaagtagaa ttggtaaaag aagaatcata cttcttccgc
    21421 atgagcaaat acgcagaccg tctattggaa tactatgaag aacatcccga attcatccag
    21481 ccagaatctc gtaaaaacga aatgatcaac aactttatca aaccaggatt agaggatctt
    21541 gcagtttcac gaacaacttt tacttgggga gtaccagtca agagtgatcc gaaacatgtg
    21601 gtgtatgtat ggatcgatgc attatccaat tatattactg cactaggata tggctccgaa
    21661 gatgaaagtc tattcgaaaa atactggcct gcagatgttc agatggtcgg taaagaaatc
    21721 gttcgctttc atacgatcta ttggccaatc atgctgatgg cccttgattt gcctctacct
    21781 aaaaaagtat ttgggcatgg ctggctattg atgaaagacg gaaaaatgtc taaatcaaaa
    21841 ggaaatgtcg tttatcctga aatgcttgtg gaacgttatg gtttagatgc actccgttat
    21901 tatttgcttc gtgctgttcc gtttgggtca gatggtgtct ttacgccaga agattttgtt
    21961 tcccgtgtca attatgatct ggcaaatgat ctagggaact tattgaatcg aacgattgct
    22021 atgatcaaca aatattgtga tggactcgtt cctgactatg cctcacttgt cactccattt
    22081 gatagtgaac tgtctacgac ggctgctaac gttgtcggac gttaccatga tgcaatggaa
    22141 aaaatggaat tcaatacagc attggctgaa atctgggtgc tgatttctcg tgcaaacaaa
    22201 tacattgatg aaacagaacc atgggtatta gcaaaagacg aagaaaagaa agctgaattg
    22261 aatagtgtca tgatccattt agctgaatct ttgcgtatcg cagcgatatt acttcagcca
    22321 atcatgacag aaacgccaga gaagatcttc aatcaactag gattggactc tgaaacaatg
    22381 aatcttgaag gattacactt tggcgaattt ccttcaggaa caaaagtagt tgcaaaaggt
    22441 acaccaatct tcccacgttt agatatggaa gaagaagttg cttttattca agaaaaaatg
    22501 tctgaaggta ctcaaacaaa cgaagataca gtcaaatggg atccagaaga aacagaactt
    22561 gtttcaacga aagaaaaaca aatcaaattt gatgtatttg aaaaagtaga actaaaagta
    22621 gcagaagtca tcaactgtca aaaagtagaa ggggcagata agttgctgca attccgacta
    22681 gatgctggag acagccaaga ccgtcaaatc ttgtcaggaa tcgctgaatt ttatcctgat
    22741 ccaagcgaat tgatcgggaa aaaagtcgtc attgtagcaa atctgaaacc acgaaagatg
    22801 cgtggacaaa tcagtcaagg aatgatcctt tctgcagaag caccagatgg ctctttacaa
    22861 gtaatcgaag caccaaaatc catgccaaat ggctcagaaa tcgcatagaa attcaaaggg
    22921 ggtatgacac tagttgtgtc ataccccctt tttccgaaat ttgagaagca attttcgtaa
    22981 attccttctt attacttgaa gccaatcata taatacagaa acagagaact atccaaaata
    23041 aatcctagaa aaagaagtac gagcttcgct gatttttgtt aatttagaat aatactaata
    23101 ggaatcgtaa aaacagaaaa aaagcataat gataaagggt cgggcagatt ttaattgatt
    23161 catctggtac tcttttttat tgtccttaca ttcataaagt ttgaatgcaa tgactttatt
    23221 acatctatat ttatatctaa cagtagcgtt gatgtgttaa ttataagttc tatcacttta
    23281 tgttcaatta gtttgtgctc ttatatgtgt gattgaaaat atagataaaa aaacaatcat
    23341 cggctttgac ttcgatgatt gttttcttta ttttgtaaaa atcctatcac ttgattttat
    23401 ggtaaccttt tatttttgtc ttcaaacggc ttggtaagag agcagtaagc agtgcaaatg
    23461 ggatcgaaaa gacataccag aaaatcgtac gtccgatacc ataatgttga tagagctttg
    23521 tcaacatcac gtgtaacaag atcaacccta aaagcaagga gccagctata aaccaatgat
    23581 tatcccaatg catgacttca ctaagtgcca tcactccaaa aggatagaaa taaaaataga
    23641 tcccgactaa ggtcatccgc cagttctttg taaatgttcc gatattcaat aaaaagaaaa
    23701 tcgctgaaag caaaataccg ataggcggaa aaagcccaac caaaaaggaa tacaacatcc
    23761 cccacaagat catcacagga cctttgatca atgcaacgat tgcgatgaac aaagtcagca
    23821 atattagttt ttgtgataac gttgcaacag tcattaatcc aaataaaatt aatgtgaacc
    23881 aatgccaatg ggactgctgt tgttctttac gggaaaaatc gacccggttg gataaataat
    23941 ctgctaattt cggataagaa cgcttaaaat ccataaactg ttcctccttc gtggaaaagg
    24001 taggtttagt atacgaaaaa taaaaaagaa atacatccgt cttaagaggg agtttttcac
    24061 tgaaagtgga gaaaaaagaa tggtaaatta aaatattctc agttatactg aaaattatac
    24121 caaatttcaa agaagaaaga ggatgggaat gaaaggcaaa ctaaaacgag aaatcaatct
    24181 gtttggcgca ttagctaccg ttatgggaac cgtgatcggt gcaggcgtct tcttcaaaac
    24241 tgctgctgtt actgctagta ctcagtctac aagtttgaca ttgctagctt ggcttttggg
    24301 tggtttctta acgatctgtg ctggattgac agtggctgaa ttagctacag caatcccgga
    24361 aactggtgga gctgtaaaat atattgaagc tgcgtatgga aaattgccca gctttttatt
    24421 aggatgggcg caaagcttga tttactttcc tgctaatatt gctgcattat cgattatctt
    24481 tgcgacacaa ttgacgaact tattacaatt atctaccgat tatcttttgt tgattgcagt
    24541 gatcaccgct gtttctgtga cagggttgaa tttgttaggg acaaaagtcg gtgcttctgt
    24601 acaatcagtg acgttgattg ttaaattaat accaattgct gtgattgtca tatggggatt
    24661 attgacccca ggacaaggaa ctgtccagtt atttccaatc gaagctggaa aagatgttac
    24721 ttttgtcgaa ggattgagca gtgcgttgtt agcaacgttg tttgcttatg acggctggtt
    24781 aggtgtcggt gcaatggcag gggaaatgaa acggccggaa aaagatttgc ctaaagcaat
    24841 tattcttggt ttgagttttg taacagtcgt ttatctattg atcaattttg tttttttgaa
    24901 aactttgccg attgatcatc ttgccggtaa tttgaatgca gcttctgaag catcagacgt
    24961 catttttgga ggaatcggtg gaaagctggt cactattggg atcttgatct cggtatacgg
    25021 tgcattgaac ggttatacac taactgggat ccgcgtgcct tatgcgatgg ctttagaaga
    25081 cgagctacct ttcagtaaac agctgacaaa gctttcaaag aagtttacgg ttccttatgt
    25141 tcctgcggtt ttccagttag cagtagcttg tattatgatg agccttggtt ctttcgactt
    25201 tttaacagac atgttgattt ttgtgatgtg gctattcagt ttactgattt ttatcggtgt
    25261 ctttatttta agaaaaaaag cgccggaatt gccacgtcca tataaagtac cactttatcc
    25321 aatcgttccg attattgcga ttctcggtgc tattttcatt ttaggtatga cgatgatgac
    25381 gcaaacgaaa ttagcattga tcggtatagg tgttaccttg ataggtatcc cagtctatta
    25441 tcaaaaaaag aaaaaacgct cagaagagta aaatatttca acaacagaca gaaaaagtac
    25501 gaactaggtc ctctagatct tcgtgctttt tctatttcaa taaaacaagt tcttctattt
    25561 tgatttttgc tacttctgca agtagtgctt atgtgctaaa gtaagaatga aaaggagaga
    25621 taaacatgat atttgattct catacccatt taaatgctga acagttcaat gaagatattc
    25681 cagaaacgat tcagcgagca caagaaatgg gggtaacgaa aatggcggtc gtgggcttcg
    25741 atacgccaac gattgaaaag tctttgcaat taagccatga ctatccaaat atatacagta
    25801 ttatcggctg gcatccgaca gaggctggca gttatacgaa agaaatcgaa aataaattac
    25861 aagaacaatt gactttacca aaggttgtag cattagggga aattggcttg gattatcatt
    25921 ggatggaaga cccgaaagaa gtccaagata aagtatttcg cagacagatt gctatcgcaa
    25981 aagaaatgaa cctaccaatc agtattcata ctagagaagc acttgaagac acgtatcaaa
    26041 tcctaaaaga agaagatgtt cgagatatcg gtggaatcat gcatagtttc agtggtgatt
    26101 atgaatgggc aaaacgattt cttgacttag ggatgcatat ttcatttagc ggtgtcgtca
    26161 ctttcaaaaa agcattagac gtccaagagg ctgcagcaaa tgtaccaatc gaacgattac
    26221 ttgtggaaac agatgcccct tatctggcac cagttccttt tagaggaaag cgaaatgaac
    26281 caggctatac tcgttataca gtagaaaaga ttgctgaact tcggaatctt ccgacagaag
    26341 aagtggctgc ccaaacatgg acaaatgcgc accgattatt tggtctaagc gaacatggat
    26401 aagatgaaga tccaagagat tatagtcgtt gaaggaaaag acgatacaag aaggctgcgg
    26461 gaagtagttg atgcagatac gatagaaaca atagggtctg caatcaatga agagatcctg
    26521 atgcaaattg aacatgccca agagactcgt ggagtgatcg tttttaccga tcctgatttt
    26581 tcaggagaaa aaataagaaa aatcattatg gaagctgttc ctcaggcaaa acatgctttt
    26641 ttgccgcgca cacaagctaa accaaagaaa aagggaagcc ttggtgtcga gcatgcaagt
    26701 gatgctgcta ttttagaagc attgaaaaag gtcgtgacgc cagacatttc ccaaacggct
    26761 gtttctgaaa tcactagaca agacctttta gactatggtt taatagctgg ggcacaagca
    26821 aaagaaaaac gagaaaaact aggtgacgag ttgcgcatcg gttacacgaa cggcaaacaa
    26881 ttggaaaaac gtttgaagat gtttcgtatt acgccgaaag aactcgccca agcaatgaaa
    26941 atagtggagg aacaacatga gtgaatatcg tgaaattgcg acaccctcta gaacaaaaga
    27001 gattttattg aaacatggtt tttcatttaa aaaaagttta ggacaaaatt ttctgactga
    27061 accaaatatt ttaaggaaaa tcgtagaaac tgcgcaaata gatgaacata cgaacgtgat
    27121 cgaagttgga ccaggaatcg gtgcattgac ggaacaatta gccaagcatg caaaacaagt
    27181 cgtagcattt gagatcgatg atcgtttgat ccctgtattg gctgatacga tgcaacccta
    27241 tgacaatgtg actattgtac atcaagatat tttaaagacc gatttgtcaa cagctgttag
    27301 agaaacattt catgaggagc ttccgttaaa agtcgtagcg aatttacctt actatattac
    27361 gacaccgatc atgatgcatt ttcttgaatc agatttggtt gtggatgaac tagtcgtcat
    27421 gatgcaaaaa gaagtagctg atcgtatctc tgctgaacca ggaacgaaag cttatggttc
    27481 attatcgatt gctgttcaat attatatgga agccagttta gcattcatcg taccaaaaac
    27541 agtatttgtc cctcagccaa atgtagattc agcgattttg aaactgacaa gaagagatat
    27601 accggcagtc gaagtgacag acgaaaaagc atttttccga ttgacaaaag cggcatttca
    27661 gcaaagaaga aaaacgcttt ggaataactt gcagcattcg tatggcaaag atgaccagac
    27721 aaaagcatgg ttagcaaaaa gcttggaaac agctggaatc gatccaaaac gtcgtgggga
    27781 gactttgtca ttacaagaat tcgccgcatt aagcaatgcg atgtcagaaa atatgcagta
    27841 aaaaaagagg ttgtgacaga agttgtgaaa aagctgtcct gcatcaagta ataagaacag
    27901 ccaaacaaaa atagttcctc atgtttttcg tttgtttggg cttattaccg cagatgcaag
    27961 cttttgaaca ccgtttaaga aagaggttgt gggcctgccg tttaactcta agtcacaagg
    28021 aacaatttca aaaaacagct tctcatgttt ttgagttagt tggtcttatt acttgatgca
    28081 agcttttgac cactattatg ccgaacaagg agatgtgaca caaattttgt cacatctcct
    28141 atttttgttt cttgatcaaa aaattcttat acttgatact cacactcatt ttgtatggat
    28201 ttagaaaaga atatgtgtct tgacagttat tagtaagtga gttatactaa gtcgacattt
    28261 atgtgtcaag acatattagg agagagaaaa aatgagaaaa aattcagttc gaaaactaac
    28321 gatttctgct ttattgattg ggatgggggt cattatccca atggtcatgc ctaaaatcat
    28381 gattccacca gcttccttta ctttggcgtc tcatgtgcca ttatttattg cgatgttttt
    28441 tacaccagga gttgcagtgg ctgttgcgct aggtacgaca tttggtttct ttttaacaac
    28501 gccggttatc atcgcattgc gagcattgtc acatcttgtg tttgcgttga ttggggcttg
    28561 gtacttgcaa aagcatccga gtatcgtttt aaaaaatgga cagtttactt ttgctaattg
    28621 gcgtttccaa gggttcaatt ttgtgatcgg tgtgattcat tctttagcag aaatgctagt
    28681 agtcagcatc ttttacttta ttggtaatat gcctgaaaca tattattctc aaggatattt
    28741 ttatacagta ttcatcttga tggggattgg tgggcttatc cacagtttag ttgattataa
    28801 tattgcttat tttatagcag gaacactcag caaatatttt gatatccccg tatttacggc
    28861 agcaaaaaaa gaaaatcgga aagagtcgat tcaagggtac gcgaagaaaa cgacatatta
    28921 gacatgatct ctcaaaaaaa aaactctcat ctagtaatgg ataataaatc ctctaagatg
    28981 agagtttttt gtcctaaaaa gagctgcacc aacatcttct ggcacagctc taacaggggg
    29041 caatttatgg aagtttgatg acttgttctg tttctttatc aaagaagtgg cctttgctga
    29101 tgttgaaggc aaggtcgact atttcgcctg gtttatggaa atctcttgca tctacttttg
    29161 agatgaactc agtagaacca gtttttgtgt aaagcatcgt ttcagctcca agtaattcgg
    29221 aaacgacaac ttctgatttc actacggctt caggtgaagc ttcaagggct acttgttcac
    29281 tgtggatgtc ttctggacga ataccgaaga taacttcttt tccttcataa cctttttcaa
    29341 ctaatacttt gttttttcct tcaggaatac gaagcttaag cccatgtcca tctgtgataa
    29401 caccattttt caatgtcaca ttaaagaagt tcatggcagg agatccaata aagccggcaa
    29461 cgaacatatt gacgggtgta tcataaactt ctttcggtgt accgatttgt tggataaatc
    29521 cgtctttcat gataacgatt cggtcagcca tagtcattgc ttctgtctga tcgtgagtaa
    29581 catagatggt cgttgtttca agacgctggt gaagtttggc aatctcagca cgcatggcta
    29641 cacgtaattt agcatctaaa tttgataatg gctcatccat taagaagact tttgcatccc
    29701 gaacgattgc ccgtcccaat gccacacgtt ggcgttgtcc accagataaa gcagctggtt
    29761 ttcgtttcaa gtattctgtc agacctaaga tctctgctgc attgtccaca cgttttttga
    29821 tgtctgcttt atcatattta cgtaatttca agccaaaagc catattatca aaaacagtca
    29881 tatgaggata caatgcgtag ttttggaaaa ccatggcaat atcacggtct ttaggtgcca
    29941 cgtcattcat gacttttccg ccgatattta gttctccttc agagatatct tccagtcccg
    30001 cgatcattcg caaagtagtt gactttccac aaccggaagg accaacaaaa acgataaatt
    30061 cacggtcttt gattgataga ttaaaatcag ttacagaata attttctgca ttgtcatatt
    30121 ttttatatac gtttttcaga gccatttcta ccataatacg ttccacactt tcttttttag
    30181 ttttattaac tatttaaagt ataaatgaaa gcgttaacta acttcaatgt catgttgaac
    30241 aagaattttt tctttttttt gtcaatgtgc tagttttaag ctttaataaa tatccataag
    30301 aaataaaaac tgatacactg gagtaaataa gtatgttaac aatatcaaaa ggagtgtctc
    30361 ttatgaaaat cgctttgatc gctcatgacc gaaaaaaacc tatgatggtg aaattagcaa
    30421 cagcgtataa aacaatattg gaaaaacatg aactttttgc taccgggacg actgggcaga
    30481 ggatcacgga agcaaccggc ttgccgatcc atcggtttaa atccggaccg ttaggcgggg
    30541 atcagcaaat tggagccatg atttcggagg atcagttaga tttggtgatt tttcttcgag
    30601 acccgctggc tgcacagccc catgaaccag atgtaacagc acttatccgt ttatctgatg
    30661 tctatgagat tccgttagct acgaatatcg gaacagctga ggtgctacta agaggactgg
    30721 aagccggctt cctagatttt cgaagaatcg tccatgaaat agacaagcat ccactttcat
    30781 tttgatggat aagcgtatgg aaaaataacg tttaatgttc gcctatatgt agctataaaa
    30841 gctaaacaaa ctgataatat gcgatataat acgaacgtat tggataatat aaacaataat
    30901 attagttttt cgtatttgca gtaaatgtga aaatatgcta tagtttctgt gggaaaagat
    30961 cccaaaaata atacagagta gaaaataaag cgttaagtgc taagaggatg ggatgttgcc
    31021 tcttggacga aggacgtaat gttcgcggtt ttattttcgc attcgctgca tgatacgatg
    31081 tagcagctct taaaggagat gctaaaaatg aagaaacacc ggttagacgc acgtatgatt
    31141 gctattatgg gcttattgat tgcattgatg gtcactttgt cccgactggt agcaatcgag
    31201 accccattta ttaaaatcag tgtcacattt attccacaag tgatcatggg cattcttttt
    31261 ggtccttttt ggtcagggat aggcgcagtt ttagctgatt tagtaggaat ggccctcttc
    31321 tcgaaatctg cattctttat tggatttaca ttgaatgcat ttattgaagg agcaatctac
    31381 ggattctttt tctatcgaaa agagatcact tggaaaaatg ccattctggc tacgcttagt
    31441 gtcacgctga tcatcaactt atttttgaca ccattatggc ttgcgttgat gtaccatgtg
    31501 ccattgttca gctgggtcgt ttgggcgccc cgcctgttaa aaacagtaat atggttaccg
    31561 attcaatcaa ttgccattta ttacgtagga cgtagtattc cttacaaaaa aattttaaga
    31621 agtttagcga tccacgctaa at
//
LOCUS       DAFBQH010000028        30585 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.028, whole genome shotgun sequence.
ACCESSION   DAFBQH010000028 DAFBQH010000000
VERSION     DAFBQH010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.028"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..225
                     /locus_tag="LK694_001368"
     CDS             <1..225
                     /locus_tag="LK694_001368"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBK5052244.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
     gene            207..512
                     /gene="kduI"
                     /locus_tag="LK694_001369"
                     /pseudo
     CDS             207..512
                     /gene="kduI"
                     /locus_tag="LK694_001369"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            532..1335
                     /locus_tag="LK694_001370"
     CDS             532..1335
                     /locus_tag="LK694_001370"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBK5052245.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(1584..2648)
                     /locus_tag="LK694_001371"
     CDS             complement(1584..2648)
                     /locus_tag="LK694_001371"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBK5052246.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(2645..3730)
                     /locus_tag="LK694_001372"
     CDS             complement(2645..3730)
                     /locus_tag="LK694_001372"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBK5052247.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(3743..4720)
                     /gene="mvaD"
                     /locus_tag="LK694_001373"
     CDS             complement(3743..4720)
                     /gene="mvaD"
                     /locus_tag="LK694_001373"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBK5052248.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(4713..5657)
                     /gene="mvk"
                     /locus_tag="LK694_001374"
     CDS             complement(4713..5657)
                     /gene="mvk"
                     /locus_tag="LK694_001374"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBK5052249.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5973..6632
                     /locus_tag="LK694_001375"
     CDS             5973..6632
                     /locus_tag="LK694_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBK5052250.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            6717..7622
                     /locus_tag="LK694_001376"
     CDS             6717..7622
                     /locus_tag="LK694_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBK5052251.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(7624..8256)
                     /locus_tag="LK694_001377"
     CDS             complement(7624..8256)
                     /locus_tag="LK694_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBK5052252.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    8407..8532
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            8576..9100
                     /gene="infC"
                     /locus_tag="LK694_001378"
     CDS             8576..9100
                     /gene="infC"
                     /locus_tag="LK694_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBK5052253.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            9172..9372
                     /gene="rpmI"
                     /locus_tag="LK694_001379"
     CDS             9172..9372
                     /gene="rpmI"
                     /locus_tag="LK694_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBK5052254.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            9425..9784
                     /gene="rplT"
                     /locus_tag="LK694_001380"
     CDS             9425..9784
                     /gene="rplT"
                     /locus_tag="LK694_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBK5052255.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            10136..11374
                     /locus_tag="LK694_001381"
     CDS             10136..11374
                     /locus_tag="LK694_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBK5052256.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAES
                     YTYNDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLL
                     PVLGVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGF
                     LMTLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSA
                     ILAVVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIP
                     TGWIIGAILCLITFLLYGNA"
     gene            11422..13551
                     /locus_tag="LK694_001382"
     CDS             11422..13551
                     /locus_tag="LK694_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBK5052257.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            13526..14215
                     /locus_tag="LK694_001383"
     CDS             13526..14215
                     /locus_tag="LK694_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052258.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEIYEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(14623..15309)
                     /locus_tag="LK694_001384"
     CDS             complement(14623..15309)
                     /locus_tag="LK694_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5052259.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(15445..15639)
                     /locus_tag="LK694_001385"
     CDS             complement(15445..15639)
                     /locus_tag="LK694_001385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052260.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(15697..16128)
                     /locus_tag="LK694_001386"
     CDS             complement(15697..16128)
                     /locus_tag="LK694_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBK5052261.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYALWMM"
     gene            complement(16132..16977)
                     /locus_tag="LK694_001387"
     CDS             complement(16132..16977)
                     /locus_tag="LK694_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBK5052262.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            17126..18544
                     /gene="pepV"
                     /locus_tag="LK694_001388"
     CDS             17126..18544
                     /gene="pepV"
                     /locus_tag="LK694_001388"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBK5052263.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(18620..19822)
                     /locus_tag="LK694_001389"
     CDS             complement(18620..19822)
                     /locus_tag="LK694_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052264.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(19809..21596)
                     /locus_tag="LK694_001390"
     CDS             complement(19809..21596)
                     /locus_tag="LK694_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052265.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(21609..22562)
                     /locus_tag="LK694_001391"
     CDS             complement(21609..22562)
                     /locus_tag="LK694_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK5052266.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(22747..23097)
                     /locus_tag="LK694_001392"
     CDS             complement(22747..23097)
                     /locus_tag="LK694_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBK5052267.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            23297..24376
                     /gene="pepA"
                     /locus_tag="LK694_001393"
     CDS             23297..24376
                     /gene="pepA"
                     /locus_tag="LK694_001393"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBK5052268.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            24519..24839
                     /locus_tag="LK694_001394"
     CDS             24519..24839
                     /locus_tag="LK694_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBK5052269.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            24861..25325
                     /locus_tag="LK694_001395"
     CDS             24861..25325
                     /locus_tag="LK694_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK5052270.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            25521..26126
                     /locus_tag="LK694_001396"
     CDS             25521..26126
                     /locus_tag="LK694_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBK5052271.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(26165..27454)
                     /locus_tag="LK694_001397"
     CDS             complement(26165..27454)
                     /locus_tag="LK694_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBK5052272.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            27882..28361
                     /gene="rlmH"
                     /locus_tag="LK694_001398"
     CDS             27882..28361
                     /gene="rlmH"
                     /locus_tag="LK694_001398"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBK5052273.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            28714..29028
                     /locus_tag="LK694_001399"
     CDS             28714..29028
                     /locus_tag="LK694_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK5052274.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            29044..29430
                     /locus_tag="LK694_001400"
     CDS             29044..29430
                     /locus_tag="LK694_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK5052275.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            29459..>30585
                     /locus_tag="LK694_001401"
     CDS             29459..>30585
                     /locus_tag="LK694_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBK5052276.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRD"
BASE COUNT         9686 a         5721 c         6239 g         8939 t
ORIGIN      
        1 ttgtgggcgc taggaatcat gctagtagca ttcatcgttg ctaaaaagaa taaataccct
       61 gttgtcgaaa aaggacagat ggctcatgct ggaaaaataa taaaagaggc gattcctagc
      121 cttctattga ttgtcatcat tatcggtggt atcttgacgg ggatatttac agcagtagaa
      181 gcttcagcga tagctgtcat ttataaatgg gttatacgat tttagaacca ggaagtgctt
      241 ggaatacgat gccttgtcat acacatgaac gtcgtatgga agcttatgtc tattttgatt
      301 atgccaatga agatacacgt gtgttccata tgatgggcaa accagatgaa acaaaacatt
      361 tagtcgtaga caatgaacaa gccattattt cgccaagctg gtcgatccat tcaggtgtag
      421 gtacaagcaa ttactcattc atctgggcaa tgtgcggaga aaatatcacc tatacagaca
      481 tggatatggt gcaaatggat caattaaaat aagcataagg gagagaaaat gatggaattt
      541 agtatggata tgtttcgttt agatggaaaa gtagcattag tcaccggtgc tgtctatggt
      601 attggatttg agattgcacg ctctttagct acagcaggag caaaaatcgt atttaataac
      661 ttgaatcaag aatcagttga tgaaggtata gcgcattata aagaagcagg catcgatgca
      721 aaaggttatg tctgcgatgt gaccgatgaa gaagcggttc aagcgatggt tcaacagatc
      781 aaagaagatg taggttcagt tgatattcta gtcaacaatg cagggatcat caaacggaca
      841 ccgatgatcg aaatggatgc ggcagacttc cgccaagtaa tcgacgtgga tttaaatgcc
      901 ccattcatcg tttcaaaagc agttattcgg gatatgatcg aaaaaggcgg cggtaaaatc
      961 atcaatatct gctccatgat gagtgaatta ggacgcgaaa cggtcagtgc ttatgctgcg
     1021 gcaaaaggcg gattaaaaat gctgacgaaa aacatcgctt ctgaatacgg tcaatacaat
     1081 atccaatgta atggaattgg accaggttat attgctacac cgcaaactgc tccattacgc
     1141 gaaacacaag aaaacggtga acgtcatcca ttcgatcaat ttatcgttgg acgtacgcca
     1201 gcagcacgct ggggcgagcc aatcgatttg gctggaccat ctgtcttctt ggcttctagc
     1261 gcatctgatt tcgttaacgg ccatgtcttg tatgtggacg gaggcatact tgcctatatc
     1321 ggaaaacaac cttaaaagag ctttagtaca ccgtttagtg gagaggttgt gaaatcagcg
     1381 gcttgaacga ataccgaagg acagctgatt aaacatcatt tattcgaaaa aagaggctgg
     1441 gacagaagcg tttaactcca agaaataaga aggaattcac gaaaattgct tttcaaattt
     1501 ttgtgaattt cagcttattt ccgaaggagt tgcttttgct tccgccgttt atacgtgttt
     1561 agagcgtggg ataaaagtgt tctttacttt tgtcccacgc tcttttctgt gcgtcttcca
     1621 tacttcacta agtcgatccc tcttgcttcg caccaatcct tcactggtcc ggaaaagatc
     1681 agtgattgtt ggtgtagggc cgcggtattc gttgcgccga ccatagtata aagcagacga
     1741 agctcttctt gccattgctt catcagtata atagtttctt ctacgccgtg attcatcaga
     1801 tgcgtcagta cggttcctga agtacccact gcatttgcgc cgagacaaag ggctttaaat
     1861 atgtcatagg cattgcggat acctccggag gctaggattt ccatagaagt gtctgcttca
     1921 tttgcttcaa gcaatgaaga gactgtcgat tgcccccagt cagctaaata actcagttcc
     1981 cgcttggagc gacgggcgtt ttcgatctgg gtgaagcttg ttccgcttcg accgctgata
     2041 tcaactgtat gaactcctaa agcagctaga tcattgagcg tttctcttgt catgccaaaa
     2101 ccgacttctt ttacgatgag ggggacgtca atggctgttt gagtctcttg aataagtact
     2161 ttccaattgg taaaatcacg gtctccctca ggcataacca actcttgagg tgcattcaaa
     2221 tgaatctgta aggcatctgc gtgaaacaat cggatagctt cttgggctct ttcgacagaa
     2281 gtccctgcac cgatgttggc aatgattttc ccgtgaggat attcttgtcg cataatcgta
     2341 taagtatcgg ctaaagaagg gtctttcaag gcagcgctga ctgaaccagt ggcaatcatc
     2401 aaatctgctt ctcttgcgac gattgcaaga tcttggttga ttttttttgt tttttcgctt
     2461 ccgccagtca tcgcattgat ataaaatggg ctgcttagtg taaacccaac tgcctgtgta
     2521 cttagatcga catcagaaac tgctatttgc ggcaaagggt tatggatgat ccgtacgaag
     2581 tcaaattcgt ttttttgttt atcatgaaag gctttagcta gtgaaacatg ttcgtctttt
     2641 cgattcatcg gttttccttt ctttgatcgc tatagacatg taacggcagt ggagtgattt
     2701 ctgctttttc ccatgcactc attaaaggaa ggatgccaga tttctggtca acaattacga
     2761 ttccgcaatc gccaccacct gcaccagaag attttgcggc tccttcatac tgttcagcta
     2821 aattacacaa tttgttcaaa gcaggcgttt cgatgacgac cccagtgatt gcagataaat
     2881 catgcagtaa ttgtcggttt tttcgaatca tctgttggat caacgttaca ttattttctt
     2941 taaacccttt gatcatttcg ttgacacatt ctgtactgtt ttttaagaaa agctgataag
     3001 ccaccatttt gtcttctctc gaacggtgaa cttgatcgac caaatcagaa gtagaggcag
     3061 ggctacccgt ccaaccaatc aataaacgta aatcttcagg agcaatcaat ggctcaatgg
     3121 atagacctgg ccaatccagg gcaagtaact cactgataga atgctgagtt tcttgttctt
     3181 ggagccaagg atggtcgaag gttgagaaag cgatccagcc accataacag ctagcagcga
     3241 tgtcgccgca ggaaccatta tcttgaactg ctaaattggc tagtgctgca atcttgaaaa
     3301 tctccaattg agataaattc aacgcgtaaa aaacattcaa ggctttgacc gttgcaacgg
     3361 tcacggcacc gctagaaccc aatccatatt tccggccatt tgaactgtct aactcacttg
     3421 ttactttcag atcataaaag gataaaagaa tgtttttttc ttgtgcatac ttttcagtca
     3481 aacgaatagc agcaaggata taatgaaaag gattttcccg aatatctaaa actaattctc
     3541 cgttgcgtct tgtccaacgt acaggcatcc cactatattg tgcagattgg atacttccga
     3601 cttttcgtgc ggattctaca gtgactgtta cgaattgatc gactgcagcg ataactgctg
     3661 gatgtcctgt ttcaacaact gcatattctc cggcaatata gagtttgcct ggtgcagata
     3721 cttcaatcat aaaaatctcc ctttattcaa taatcgcaat tcctggacca gcatgggcag
     3781 taattacttg ttctttgcta aataatttgg taaatgtttc ttgtacctca gataaatggc
     3841 tgttttccac taaaactttg acattagggc cagcatccat cgtaaagtag catggaatgc
     3901 ctttttttct caattgccgc accgcatcca tcgcttttaa gctgtcagga gaccagtaag
     3961 tgaatggagg ctgggcagct aaagtcgttc catgcatttt tagtccgttt ctttccatcg
     4021 tttctccgag aagttggaaa tcttttgttt tgattgcttg tttcaattga tatagatccc
     4081 cttcaacgga atctaaccag ccttgataaa aactggatgt ttcgactgtt cgacgcatcc
     4141 catttctgct ggacacttct tttttctggt cgttgatcaa aacgaaaacc attgctaaat
     4201 cgtcttcgaa agaatcggaa gggactggct tagcgtaaga acttaagtcg tcatggcctt
     4261 tttcccattc aacgaaacca ccgaaaatac ttcggcaagc tgaaccagac ccgcgtcgag
     4321 caaatcttga aagagagaga tcgtctaatc caagcttaag agcagtattg caagctcctg
     4381 ctaaagcagc tagcccgctg gcagaggagg ctaatccagc tgcggtagga acgaaattct
     4441 gactgattac tgaagctttt agtgagaggc cggcttcttt acgaaaaagg tcaagaaact
     4501 gactgacttt ttttgttgct ttttcgtctt gtaaggtgcc atctagataa aattcatcta
     4561 ccatatagct atcagaaaag atgacttctg tctctgtata aaatgcatcc aatgttaacg
     4621 aaaggctgtt gttcattgga aggatgagtt cttcattttt cttaccccaa tatttgatta
     4681 gagcaatgtt ggtatatgcg cgtgctttgc ctttaaacat aggtatgtac tcctaatcct
     4741 tgtatccaag tttcagcagc acccgcatct tcaagtgctt gactgatttc ttgggctttt
     4801 gtttttgttt gtgctaatgc aatcatacag ccaccgcgtc caccgccagt taatttggca
     4861 cccaatgccc cggtatcttt tgctgtttgg atcaatagat taagaaaatc attgctgatt
     4921 gtcagctttt ccagaagtga ctgtgattca tccattgtct gtgccaacgt ttctggtgaa
     4981 ttctcaatga ttgcttgttt tgcttgcttc gtcaagtatc ctaatttttg gatcatttgt
     5041 ccagtttcat gaggctgggt ttcaaagagg tgagcgacat ctttgacggc ttctcttgtt
     5101 tgtcctttga ttcctgtatc agcgacaatc aaaaaagcat cgatgttcaa agaaaagtag
     5161 tcaaaaggat gcccttttgt aaaataaatc ggctgcaagc tgctagtagc ggctgcatcg
     5221 attccactag gattaccgtg ggcgattttt tccgaaagct ggacattttc taatagtatt
     5281 tcacgagaca aaggaaatgc taagtaatca taaaaagcac gagtgactgc tgtggcgact
     5341 gcagcgcttg atcccattcc acgttcagca ggaatcgtgc tttcaatggt cagttgcaag
     5401 tttttttgga tgtcatgctg tttttttaag tgaatgaata attttttgat attttttaat
     5461 gcatgaggag cttcggctaa atttcctgaa tagtaggaag aaaccagttg atcgtccatt
     5521 gcatccagct cttcaaggaa tgcggtgact tttgttgcat agaaaggaaa ggcaatcgct
     5581 ggttctccat aaacaaccgc gtgctcgccc atcaatatga tctttccgct tgattctcct
     5641 tggccatagt ttgccataat ctaactcctt ctcacgtaaa agtgattttt aagcaaaaaa
     5701 taaagcgccc aaaacgagtt gcgctgcttc acgaattttt ttgtccacta agggtctcaa
     5761 aaaacattcc tcctttattt tactaaaacc atctcagaag taaaactaag aatacgtttt
     5821 ctaaagaaaa aacacttttt tcttaagaat tttttgactt tttttcttct ttgactagaa
     5881 aaaatacaat tgacactact agatattggg ggtatattta aaaaaggttg gagttaaaca
     5941 caatatttag tgatgaagtg agctggaggt aaatgagcgt gatcgtttta gcaggtacaa
     6001 ttggtgccgg aaaatcaagt ttaacagaaa tgatggctga acactttgat agtcaggcat
     6061 tttatgaatc gattgatgat aatgaagtct tgcccttatt ttatgcaaac cctgaacagt
     6121 atgctttttt gctgcaaatt tattttttga ataaacgttt tgcaagcatc aaacaagcaa
     6181 tgaaagatga caataatgta ttggaccgtt cgatttatga agattcactg cttttccatc
     6241 taaatgcaga tctaggaaga gcaacagaaa cagaagttcg tgtgtatgat gaactgctgg
     6301 aaaacatgat ggaagaactt ccctatgctg cccataaaaa acatccggat ttattagttc
     6361 atattcgtgt atcttttgat acgatgttag agcgtatcga aaaacgtggt cgttcgtatg
     6421 aacaattgtc ctttgaccca agtctatacg attattacaa agaattgaac agacgttatg
     6481 atcaatggta tgaagaatac aaagaaagtc caaaaatcca aatcgatgga gaccgctata
     6541 attttgtgga agatcctcaa gcaaaagaag aagtcttgaa aatgatcgaa gaaaaattag
     6601 cagagattcg ccaaaccaaa gtatccattt gatttacagt tgctcaaata aagggtaaac
     6661 ctttatttga gcgacttttt tctttatact ggaaacaaat gtcaggagga tagagtatgc
     6721 gggaaaaaca ctattttaat caagaccgta atttttggat caatgtacag caaaacgatg
     6781 tgctagctgt taaaaactta aaagaagaat atggcattag tgacgagatg ctgacttatt
     6841 ctctagataa aaatgaacgg gcgcgcgtag agtacgattc ttttgatgaa gcgttactgc
     6901 ttgtatcaaa tgttccgcac cagcaaaaag tcgataatca ttatgaaact agtcctatcg
     6961 cttttatttt aaaagaagac ggtttgttta catttaccac gccgaatacc gagtacgtga
     7021 ttcgactaat tcgatcgctt ttggaacgga tgccagacat gagtgtatac agtttattgt
     7081 ttcgtacatt gtttttgatt tctgattcct tttttccttt aatcgaggaa gtgaatagtg
     7141 aacgtcagcg gcttaatctg aaattaagag aaaaaacaac gaacaagaat ctgctgcaac
     7201 tttcagattt agagatcggt ttggtttact tagttacagg aacaaaacaa aatgtagtac
     7261 ttttagaaca aatcaaggca ctggctattt atcgcaagtt gtcagaaaag gaaaaagaag
     7321 agttggacga tgcactgatc gaagcacgac aagcagttga aatgacgaac ttagcttcgc
     7381 agattttaga ccagctctct ggcacttata ataatttgct gaataacaac ttgaatgaca
     7441 cgatgaaatt tttgacggtc tggtcgctga tcttgaccgt tccaactatt gtgacaggat
     7501 tcttcggaat gaatctccag ttacccttta cccattccgt tttcggatgg gaaatcgcct
     7561 tgatcatcag tttggtctta tctatttgga tgcttattgc actgtggcgg cggattcgtt
     7621 gatctagtct ttgatctgca aaccaagggc ttgggcgatc acaagtgctt gctcttgatt
     7681 gacgattagt cccgccaaag aatcttgaga taaagcgatt gttgtgaaga aattagtcgt
     7741 aaagtctaat cccttcagag aagttcgaaa ccagttcgaa ccggttaact gattgtcaga
     7801 caaaaaaagg tttttccaag tgacctcata aaattcgctg tcttctaatt gacaatgatc
     7861 gaaggagaca gctttcaagt ttgtactact aaaggaagcc atcgaagcaa tacaatcagt
     7921 gaaagtacag tctcgaagat aactttcggc aaaattcgta cctgttaatt tacattgatg
     7981 aaacacgact tggtggaaac tgccaccaat ccactccaga ttggaaaaat cacatttttc
     8041 aaaaatgaca ttactgcatt caaaatgacg taaacctgtt ttttgcatcg taagtttttg
     8101 gatatgactc tctcttaata ccatattttt ggcatcgaga tagctttggt tacctccgtt
     8161 gatcagaaga ccagagaaga tctcctcatc ttctaaataa aactctccct ttgtaagaag
     8221 aggaagagaa ggtggaatag gttcgatttt tttcatgtgg gttcacctcg tttttcttta
     8281 gtatagcaaa gaagcgggaa tcactgcaag aaagcctgct aatctaatca ttttcagaaa
     8341 aggaagacaa actgtatgaa aaacattgac aaaaagatta gtttttgcta tactataatg
     8401 gttgagaaat aaaagcagaa gcacccgctt ctcgccttag ttgataatgt cactgggcta
     8461 gatagttttc actggttcta ttaggtgaag tacgcgcggg ttctgtaaga gaactcgttt
     8521 ttttattgcc gtttattcgg taagcttttc tttctctcat aatatttgga ggtgaatgac
     8581 catagcaaag gatatgatgg ttaacgacgg cattcgtgca cgagagttac gattgatcgg
     8641 ttcagatggt gagcaattag gagttaagac aaaagcagaa gcattgaata ttgcagaaca
     8701 agcaaacttg gatcttgtgt tagttgcccc tggtgcgaaa ccaccagttg cgcgaatcat
     8761 ggactacggt aaataccgtt tcgaacaaca aaagaaagac cgcgaagcgc gtaaaaaaca
     8821 aaaagtgatc aatgtaaaag aagttcgctt aagtcctacg atcgacttga atgacttcaa
     8881 tacaaaactt cgtaatgcac gtaagttctt ggaaaagggc gataaagtga aagcttctat
     8941 ccgtttcaaa ggccgtgcca ttacccataa agaaattggt caaaaagtct tgaatcgctt
     9001 agctgaagaa actgcggata ttgcaacagt ggaacaaaaa gcgaaaatgg acggacgcag
     9061 catgttcttg acgctggcac cgaaaaacga cagtaagtaa tttgaatgac taactggctg
     9121 ttggacagcc gatagtcgga taacgaaaga ttttgaggag gaaatatagt catgccaaaa
     9181 caaaaaacac accgcggatc agcaaaacgt ttcaaacgta ctggtaaagg cggattgaaa
     9241 cgcttccgcg cgtttacaag tcaccgtttc cacggcaaaa caaaaaaaca acgccgtcaa
     9301 ttgcgtaaag caagcatggt ttctagtggc gacttcaaac gtattcgcca acaactagca
     9361 aaaatgaaat aagaagtaca aataacagaa tctgaagatt tatttttatt aggaggaatt
     9421 atacatggca cgtgttaaag gtggaacagt aactcgtaaa cgtcgtaaaa gagtgctaaa
     9481 attagcaaaa ggttactacg gttcaaaaca cactttattt aaaacagcaa aagaacaagt
     9541 aatgaattca tacaactacg catatcgcga tcgtcgtcaa aagaaacgcg acttccgtaa
     9601 attgtggatt gctcgtatca acgcagcagc tcgtatgaat ggcttgagct actcaaaatt
     9661 aatgcacggt ttgaaagttg ctgaaatcga catcaaccgt aaaatgttag ctgacttggc
     9721 tgttaacgat gcagcagcat ttactgcatt ggctgaccaa gcaaaagaag ctttggctaa
     9781 ataagctaaa caattataaa gaggaaagaa gataacgaaa agttatgttc tttcctcttt
     9841 ttttatttac aatcctccat caaatgaaac aggacaataa cgtataagtc taaaataaca
     9901 taattaaggt tttttaattg aaaactgaac atttatggtc atcctttaat gaaagcgcta
     9961 actacataag ttagaatgag tttgactgat atattagttt gcttatcaga aaaacaaaat
    10021 ggacggagga ataacatgag tgatattatt attggtattt tgttagttat ctcattcttt
    10081 ttcatggtct ggtactgtgt aaaaggctac aacttgatgg tcggcttttg tcatcatggc
    10141 tactgtatgg atgggattag cgctagtcgg aaacacgttt tcaccaaact cggcgatgga
    10201 agggcaagga gtaattgatg tgttgacgca tatctatacg acggggccag ctgagtacgc
    10261 caaatcgatc ttggtaaatg tcttctttgg tgcctttttc gggcgtgttc tagtagatag
    10321 cggaatcgca gctaccctga ttcgaaaggt agttgaactt ggaggggaca agccaagaat
    10381 cacgatgagc ttactatgta ttgtaaccgc tgttatattc atgtcgatga caggaatcgg
    10441 accagtgatt tctattgcag ttattgtgtt gccgatcttg atgtcattag gtatttcagt
    10501 accggttgca ctgttctcat ttatgggctc gattatggct ggaatctttg ctaatatcgt
    10561 caatttcaaa caatatcaaa cgatttatgc tggtttcaat ccagcggctg aaagctatac
    10621 ttataatgac tatttccaaa ttggtatgat tgggatgatt gtaagcttag ttgtggtact
    10681 aacggtcgct aatatatcaa tgaacaaaaa gaaacgctat gcaatggcag caaacgtacc
    10741 agctgaaggt ggcgacgcac caatgatctc atggttagcc gtattattac cagttttagg
    10801 ggtcgttctt ttagatcttc aaatcatctt aggttttatt ctagcaggta tctgggcatt
    10861 gctgtttact ggaaaattgc gcggaggtta taaagaaatc tgtcgtcagt ttgcgaaatt
    10921 atttacagat ggcgcagtcg atgttgcacc aatggttggt ttcttgatga ccttagcgat
    10981 gttcaataat tcagctgcgt atgcttctcc ttatttttca gctatttttg gagattggat
    11041 accgcaatca ccattagtct tggcaatcgt atttgctata ttgacaccac taggattttt
    11101 ccgcggtcca atgaatctag tcggttcagg ctcagctatt ttagctgtcg tgttagctgt
    11161 taatccaacg atgtcgccag cctttttgtt cccgttattt gcaattacaa cgattgctcc
    11221 tcagcattta gatattacac aatcttgggt tgcttgggga ttaggttata caaaagtaac
    11281 ttctcgagaa tatatgaaga aatccatccc aactggttgg atcatcggtg cgatcttatg
    11341 cctgatcacg ttcttattgt acgggaacgc ctagtaaaca aaacgtcgat aaaaacgatt
    11401 agataaatgg aggaaaagaa aatgaaacgt tttgtgcgaa tgggaattga tgttggcggt
    11461 acacatacga aagcagtcgc aatcgataat gcgacgcacg aaatcatcgg aaaatcttca
    11521 gtgaaaacaa cccacgatga tgtccgtgga gtagccgcag gtgtcgttca gtcttttcaa
    11581 aattgtttgc gggaaaataa tatcagtcca gaagatgttg tgtttgttgc acattctacc
    11641 acacaagcga ctaatgcact gattgaagga gatgtagcaa aagtcggcgt aatcgggatg
    11701 gcaaaaggtg gattagaagg ttttttagca aaacgacaaa ctagattgaa cgatatcgat
    11761 ttagggaata agaaaaaaat agagattgtc aatgcattct tacctgtcaa aaacttaaat
    11821 gtagaccgtg tttcagaaac gatttcttct ttagaaagag aaagagcgga ggtcttggtt
    11881 tcttctatgg catttggagt agataatggg gaaccagaac gagttgttta tgaagcagca
    11941 tctgtgaaag cgatccctac aacgatggct tctgacatca caaaattata tggattgaca
    12001 agaagaactc ggacagcagc aatcaacgca tctattttac caaaaatgct agatacggca
    12061 acctcgacag aagactccgt acgagaagca ggggtcaatg tttctttgat gattatgcgc
    12121 ggagacggcg gagtaatgga aatcaatgaa atgaaaaaac gacctgtttt aaccatgctt
    12181 tccggtcctg ctgcatcagt catgggctct ttgatgtact tgagagcgtc taatggtgtt
    12241 tattttgaag taggcggaac aacaaccaat atcggggtca ttaaaaatgg acgtccagca
    12301 atcgattatt caattgtagg tggccaccca acatatatct cttcattaga tgttcgggtt
    12361 ctaggtgtgg caggcggttc gatggtacgg gctaatcagt caggaatcat cgatgtcggt
    12421 ccacgttctg cacatattgc cggtctagat tatgcagtat ttacggagac agaaaaaatc
    12481 aaaggtccta aggttgaatt tttctctcct aaagaaggag accctgctga ttacgtcaag
    12541 gtagtgatgg aagacggaga agaagtgact attacaaata catgtgcagc caatgttctt
    12601 ggattagtgc aagaagaaca tttttcttat ggaaatgtcc catctgcacg aaaagccata
    12661 caggcattag cagactattg tcatacgacg gttgaagaca tcgcagagca gatcatggaa
    12721 aaatcctatg ctaagattga accggtgatt ttagaattgg cagacaagta tcacttggag
    12781 aaagatcaga tttctcttgt aggtgtaggg ggcggcgctg cctcattgat tacttacttt
    12841 agtaataaaa tgggtgtgaa atattcgatt cctgaaaacg ccgaagtgat ttcttctatt
    12901 ggtgtagcac ttgcaatggt ccgagatgtt gtagaacgta tcatcccttc gccaagtaaa
    12961 gaagatatac gatcgttgaa aaatgaagcg atgaataaag cgattgaatc aggcgctaca
    13021 ccagaatcga tcgaagtcca cgtagaaatc gatccacata cttcaaaagt aacggctatc
    13081 gctactggtt ctacggaagt gaaagcaacc gatctaacca aggagatcac aacagaagaa
    13141 gcactagagc ttgctgcaga ggatatgcga ttaaataaaa atgaagtttg cttattggaa
    13201 aatacacctt tcttttatgt atgcggcgaa cagaatcgtt caaaaaatgc tggaagtctt
    13261 cggatcatcg atcaaaaagg atttatcaag gtccaacgtg gacatgcttc atgtatgaaa
    13321 acaactgcag ccaattatat gacagctgtc gaacaacttt gggaagatat ggcagtttat
    13381 caaactgaat tgattgcccg accagaattt tatctctgct taggagcaag agtctctgat
    13441 tttacagcga cggatctgga acaactacag cttttgatgg atctggaagt atctactatg
    13501 gaaccggagg aagaagtgat cgtcgttgcc ggaaatatca aacaaacata agttagaaga
    13561 gttactaaac gaattacaac agatccccga agaaatttgg ggattctacc agtttcggcg
    13621 agatttattt tggaaaaaga ttcctttgtc caagcagaaa attcttatac aacaatcaat
    13681 cgattgtggt atcgaaacgg catgtagcat caaaaaaaag tacccttttg ccgatgtagg
    13741 agaaatctat gaacaaatgg ctgttccaat cgtttcctgt gaatcagagc aaataaacga
    13801 acgaatcact tttgcaactt atgcagaaga tgaagggatc cgattgatga cggagccgtt
    13861 agaaaaatta aaatgttcag gactagcttc gatctctaaa gaaacagcgc aagctttgat
    13921 tattggacat gaactatttc accacatcga agcttctgtt aagggaattt atacgcaaag
    13981 cgaaaaaatt gtgctgtggc ggttgccttt ttatacacat caatcaaaca ttcgtgcatt
    14041 gagcgagata gcagcgatga gtttttcaaa agagatgaat caaagcgatt tttcgcctta
    14101 tgtattagag gcagtcttac tttggccata taatgaaatt cgtagccaag ggatcttaga
    14161 agaaataaaa gaaatagaaa aaagatgtac ggagtatgat ttagattaca aataaagaga
    14221 ctgtgacaaa agtcgtgtca cagtcctttt tttccgaata aacggtgttc tcctgtcaac
    14281 tcctcggtca caagtcaaaa tattcaacaa acatgagaag ctgttttttg aaattgttcc
    14341 ttgtgactcg gagatgaacg gcaggctcac gacctcttgg aaaaaggtgt tcaaaagctt
    14401 gcatctgcgg taataagaac agccaagtta aaaacataag aagctatttt tacttggctg
    14461 ttcttattac ttgatgcagg gcagcttttt cacaacctct caataaacgg tgtgcaatca
    14521 gctgaccctc ggtattagct caaaaaaaac ggcaaaatat gaggaactat tttgccgttt
    14581 atttctctaa tactcaggtc aaaacgctga ttccaaaacc tcttatttga tgggtctgtc
    14641 ctgaatatct tcaaaataat cggggtaatt atgaatcacg tattcttgct cctcgataat
    14701 catatgtaaa tgcatcgtgc aaagaaatcc gacttcatca aagttatgat caatcattga
    14761 ttgaagcatt gtctgatgtt catctaatac tcgaccccag tctaattctg aaatcgtcaa
    14821 acgtaaccat ctgaaacggt tcaagtgcgt actataactt tctagccaat cccaaatttc
    14881 tcgttttcca gcaatttcat aacaagttcg gtgaaaatca gtatccaaat caaaaaaagc
    14941 ttttttattt tgagcgatca ctgcctgatc ggtttgttct agtatttttt ctagtttagc
    15001 ttgatcttgc ttggtcattt tcgcactgca ttcttgcagg atcggttttt ccaacttttc
    15061 ccgcgtataa cgggctaatg aagcagatcg gatatcgatt tttgaaacat aagtgccgga
    15121 ctgtggaatg gtttttacaa gctcttgatt gcgcagttgg atcagtgctt ctcgaatcgg
    15181 tgtacgcccg atagctagtt gttcttctaa cactttatcg gagattttac ttccaggtgc
    15241 taactcggaa taaatgattt gtttacgtat agattcataa gcttgctgtt gcaaattatt
    15301 gataaccatt taaccttcct ttctatgcag atataatcta ttataacata aatcgcttac
    15361 aaatcgtgtg ggcataaaaa cacagtctgc aaaaaaagcc ctgataggca tgcctattag
    15421 agcctcgaaa agaaaaatat tgaatcaatc tggtcgtacc tcttggatcg gtttgacacg
    15481 accgccgatt cttttagcaa aagcttcgga ttcttttttg tctttgaaaa tgagcagatt
    15541 gtctaagttt ttttcccgag tgcgacagcc agttttatcg atgtaaccgt gcattacttt
    15601 aacgacatac atggggatca cctgcttttt tcttttcatt atacgaacta ctcgacacaa
    15661 tacaagaaaa atgcttagac ggtatttatt aagaatttac atcatccaaa gagcgtacgc
    15721 ttggaataac tgttctaact ccggattgac tttgctcagc ttcactaatc gttgcgaatc
    15781 agaaagtaaa aaacagtgtt tactactgcc agtagttttt aattttttgt cttcagttcc
    15841 cacaatttcg tagctaaaat ccaaccgtgt tcccgtatac ttttggatag ttgggtggat
    15901 gctcactttt tctccgtaat ggatcatttc tttatagctg caatttactt ccagaacggg
    15961 gatgatgatc ccagattctt cgattttttt atagggaaac cctagatgta ccaataaggc
    16021 tacccgagcc tcttcaaacc agcggatata attgctgtga tgaataatcc ccatttgatc
    16081 tgtttcataa taaaaaggtt ctcgcacata acctgtaaat ttctccatga ctcatgcctc
    16141 gttttccatt gattttttac aaaaataatt catccacttc ggaagtgctt cgctgatttg
    16201 tgtcggcaaa acaatatatt gttcttttgc cagctggtct ccaatgaaac tatgaagata
    16261 aacagctgct gcaatcgttt ggtcattttg aggaaactgg gctaaaaatc ctgtgatcat
    16321 cccagctaaa gtgtcaccca tgccgcctgt tgccatagca gcagaaccta aaggattggt
    16381 gtagaagtgc tggtgaccat aaatttttgt ctcatgactt ttcaaaacga gcacagcacc
    16441 gatttcctgt tgcttctttt tgttttcttt atcccgttgt tcttgtatcg gtagagaact
    16501 caaccgttgc cactccattt gatgaggtgt aagtacagtt tgttcgggat agggtagcaa
    16561 aagatgatta gcaacaagta aacttaaagc agagccatca atcaccagcc attgttcttt
    16621 tgattgtttt tctagtactt gtttgaaacg ttccaaactc ttagaagaag tacccaatcc
    16681 gggaccaatc aagagtacat ctgcttgctt tattacggaa agaaactggt cagtatccgt
    16741 ccaatctaca atcattgctt ctggcagatg actgtgaaga gcactccgat ttttttcatc
    16801 agttactaca gtcgtgagac ccgctccact gtgtacggca gctaaagcac tcataatgat
    16861 tgctccgcca tattgttcat tgccgccaat caaaacgata cgtccaaatg ttcctttatg
    16921 tgaaatcttt ggacgaggtg tgatgactct tttcactagt tgttcgttta tcagcatcga
    16981 aaaacctcct tttactcatt atagcgtata ttttttttag agaaaaacag ataagaaaag
    17041 gaattgtcag attttttgaa ctattttttt ctgtaatttg tggtatcatg aacacgaaga
    17101 cttaaataag tggaggatta actttatgac aattgattgg caaaaagaag tagaagcacg
    17161 taaagaagac ttgcttgaag acttgaaaaa tcttttacgt gtcaacagcg agcgcgatga
    17221 ttcaaaagta acaccagatg caccatttgg acctggacct cgcgacgcat tgaaacatat
    17281 gttagcttat ggcgaacgtg atggatttaa agtaaaaaat gtcgataact atgcagggca
    17341 tattgatctt ggcgaaggag acgaaacgct agggatattt ggacacatgg atgtcgttcc
    17401 tgcaggagat ggctgggaca cagatcctta tgaaccagta atcaaggatg gaaagatctt
    17461 tgcacgcgga tcaagcgatg acaaaggtcc aagtatggca gcttactatg cgatgaagat
    17521 cattaaagaa cttgacttga aactatcaaa aaaagttcgc ttcgtagtcg gcagcgatga
    17581 agaaagcggc tggggcgata tggcttatta tttcgaacat gaagaagaac cagattttgg
    17641 tttctcacca gatgctgaat tcccaatcat caatggtgaa aaaggaaatg tttctttagc
    17701 tttacgcttc aaaggcgaca atgcaggaga ttatgtcttg aaatcttttg tttccggact
    17761 tcgcgaaaat atggtgccag gaacagcaac tgcagcactt caagtaccaa gtgcagatgc
    17821 agctatcgcg atggaagaag cattttacca attcatcgaa gcaaatccag taagcggaac
    17881 gattgaagcg gacaatacgt atgtaaaaat cgaattagtt ggtaaaggcg cacatggtgc
    17941 aagcccacaa tctggtatca atgcaggttc tttcttggca ttgttcttag ataactatga
    18001 attcctagga tctgctaaac aatttatcca tgtagcagct gcttatgtcc atgaagattt
    18061 ttatggagaa aaactaggag tcgcttatga agatgaaaaa atgggcaaat tgacaatgaa
    18121 tgctggacta tttgcttttg aagaaaatgg tacagaggaa gcgaacttca tcaatatgaa
    18181 tttccgtttt ccaaaaggtg taacagtaga cggattgcaa agtgacatcg aacaaacagt
    18241 ggggcaagaa ggcgcaacag ttactcgagg tgctcgcgta atggaaccac attacgtacc
    18301 aatggatgat ccattggtgg ctacattatt gcaagtctat gaagaccaca ctggagaaaa
    18361 aggctacgaa caaatcatcg gtggcggaac ttacggacgt ttgttgaaac gtggcgttgc
    18421 ttatggagcg atgttcccag gatatactga tacaatgcat caagcaaacg aatttatgag
    18481 tcttgatgac ttgttcagag caacagcaat ctatgcagat gccatctatc gtttggcgaa
    18541 ataaaaatac taaaaaactc cggctatctc tgacagctgg agtttttatg tttaaaaatg
    18601 aattagtaca tcggtattct taaaagtaat ttagttcgtc ttttgatttc tcaggaggga
    18661 tcaaaaaggc gaaagcacta agtatagcaa aacttgtgat gatttgcgta cgatacgtaa
    18721 accaataatg aatctgtgag tgattagcta aaatattgta ccaaatatac ggcgttacag
    18781 cagctaaaac aaacaaaact agaagttgga acggacgcca gctatttcgt ttacgataag
    18841 caaaatagag aaaaacaaga caagtaatca atagcaataa tattgctact tttggataca
    18901 tcaatctaat attgcttttc agcatctcta atcgatgcaa cgggtatttg tcgtttcctt
    18961 ccgttcgaaa gaggatttgg ttgattgcat cttttacaat gtttttgttc aatatgatcg
    19021 atgataggat ccatttagcc acccatgtca aaccatagcc taatccccaa aagataccgg
    19081 taaaaacaaa actgcctact tgtttgctca atgaactgcg tttatccatt aaatacttat
    19141 ttgttacata ataaataatg atcacaggta tcccccaaga gatgaccggt gttgtcaaca
    19201 agtcaaaaaa gttagctaaa gaaccaatga caaagaaata caagaataat tgttggttgg
    19261 tgttggagag ttgtggtttt gttaaaaata gtaatacaga caataaagtc aaaatccaaa
    19321 tatttgaaaa ttgcatactg aagaaaaagg ttgcagcatt tcctatagca agcgaaatca
    19381 acaaggctgt cgttaaatag atgtcgctac acttggttaa aaagtagctc gtcaatccaa
    19441 tcagcagaaa aagaatggct gcgtaaatca ttcgaatact ccccaaactc atccctagca
    19501 gtaaaggtcg taaaaaaagc tgataaccat gccaataacg gggataatcc gccatagaca
    19561 tcgcgttctc caaggagttc aattcaggat ctgcttttgt ttttcgtatc atcaaatgat
    19621 cagtaaaatt atctaaccga gtgcctagca tatttccttg attgatagaa ggatacattc
    19681 cttctttttc aaccatctgt agagattttt ctgcattcga ttcaatagag tgctgcggaa
    19741 tgaaaaaagt aattgtcagt agtacgcaat agctagcgat caagagaaca aaagcaaaaa
    19801 atatcttttt aaatagtttc atgagtatct cctatatctg ttttcatttt ttctctttta
    19861 tctagtaaaa aaggagagag gatataaaca ggtagaccta atgctaaaac aaacacatac
    19921 cgtaaactaa aagccacagg agtagcaatc ataatagtag caaaaacaag cataccggga
    19981 agaaaaagga gtaaataccg atattttttc ttaaagattg ctaaaagtgc actgccgata
    20041 gctaaccaca gtaatgtgcc ggaaccaaag aaatcacgag catcgatgat ttttttcatt
    20101 cctgacaatc cagtaagatg ttcaatcaaa tctacttgct tcataccata attattcgtg
    20161 tttacacgag tatctaaaaa gccgtaacta ttttttgtac caggtaccca gaacccgaac
    20221 gtttctaaaa tataagcatc tgtatagatt tttatattac ttggtaaatt tcttgcccat
    20281 agtgatagaa attctttttt atgtgtattc aaaaactcat tgttaaaatt tttatgccac
    20341 ttgattgagt ctgcaagact aggagaatac gcttcataat cttttatcgg gagtagctga
    20401 ttcatgaatg ctttatcttt cttagacaag tcaccatcca taacaatagt tctagaaatt
    20461 tgctgcaaag gaacacctaa ttgttcagca gtttctgtag gaatagcata acgagccatc
    20521 aacgggtgga tgagtagtcc agcaatcact gtataaccca gcaccacttg aaagaatgct
    20581 tgataccttt tttgtttcaa aataatcaag ccgcaaatca aacctgtgcc ggttactaca
    20641 taaatcccat tatttcttag ataggtggtc agtaaagcaa ggatgattac gtaaatctga
    20701 ttgagaatcg ttggcttttc tcgttgaaaa ataaagtcaa aaaccgctaa ggaaaataaa
    20761 aagcaagcga tagagaacaa agtatctttt tgcatgttca aggcgtagat tggaaaaacc
    20821 ggtgcaaatg caaaatagca taaactaatg agtaccggaa aaaaatggat gtttcttttt
    20881 aacagccagc aaagagaata acttatcgct gaagctgtta tcaatgtttg aaaaatcgaa
    20941 aataaaaata caccgtgatt gatcgtatca gttggtaaat catgaacaaa tagcttgacc
    21001 agtaaggtga ataagactgg atgatgattg cttataggaa catcacccat agattgggcg
    21061 accgaactta acgaatcagg caaaaccact ccaggataca atgccaacag ataaggaagc
    21121 caacacagaa ctaaaaagac agtaaagaat aaaaaatatt ttcttgaaga gaaagcctgt
    21181 gtgttttgag cagtaaaaaa tgtttccagt tttggcattg ataaataaat cgattgtaaa
    21241 aataaaaaag aatacacaaa aatggagaaa taaacaagaa agtaaaaaat acttccatca
    21301 gcaatcgttg tctttgtata gtttgctgag acatactgaa attgtatctg tctgccaata
    21361 gaaagtgtaa gagcaaaagc tgcagaaaaa aacaatcggg caatagctgc aactttgttt
    21421 tttattgaaa acaactctat tccatattgc ttaaaagcaa gtagcaaaac catggctaaa
    21481 ccaatgatta ctgttggatt caaagttcct agaccataag aagtaaaaaa agcaaaacct
    21541 gacgcaaata aagttgcaaa aaaagtagga aatagattct cctttttctt atgcataaaa
    21601 aaggcctcct acttcatttc tttattttga tattcgtggt agacatggta taaattcaat
    21661 tcatattctt tcttggcatt tgtgacgacc gtatccaaaa tcaaccctgt tactaatgaa
    21721 agaagggcta aagtcatcaa tccagtactt aaaatcgcag aagggatgcg tgtaataaag
    21781 cttgtcagca tatattcacg aatcacagga atccccatga ccaatccaaa taaaaagaag
    21841 aagatggtcc agatcccaaa gaaaagcaaa ggtttataat ctttgcacat tttgatgatc
    21901 atcatgatca ctttgaatcc gtcagagaat gtattcaatt ttgattcgct accttcagga
    21961 cgatcgcgat aatcgattgg aatctcaacg aacttgaatt ttttatctaa agcatgaatc
    22021 gtcaattctg tttcgatttg aaaaccagaa ctcataatag ggaagctttt gacaaaaatt
    22081 cgattgaatc ctcgatagcc ggtcatgaca tcgcgaattt ttgttttata taagtaagta
    22141 attgtatttt taaccagatt attcccaaaa tcatggaaag gacgcttatt ttcatcaaaa
    22201 taggttccat ttgatagacg atcgccaatg accatatctg cttcgccaga acgtaatttc
    22261 tcaagcaaac catgtgcagc ttctgcagga taagtatcat caccatctac catcaaataa
    22321 taatcggcat caatatcaaa aaacatttga cggattacat tcccttttcc ttgacgtggt
    22381 tctttcttta cgattgctcc gccttgaacg gccaattcgt atgttcgatc ggtcgaatta
    22441 ttatcataga cataaatatc tgcttctggt aattcccttt taaaatcttc aataacagtt
    22501 gaaattgtcg cctcttcgtt ataacaagga atgagcacgg caattttttc acgtgattcc
    22561 atttgtcact ctccttatat aataaaacta atcttatttc aataataccg tataataact
    22621 acaaatacta gctattccaa gaggaaatag ccctattttt ataaaaatca cccactataa
    22681 aagaaggata ggatacctct cttttagata gtgggtgcgt attcgttatt tcattttttt
    22741 gacgtattaa tctattgcgc ccttttttct tgaaatgtct aatacggttc ctgtatgagc
    22801 atctgctaaa aattcataat aaacagtttc gccatcttct atccgggaaa gcccaccccg
    22861 ataagcactc gaccgaacgg cgaatttccg aataggttct ttttcaaaag agatccaagc
    22921 accttcgatt gcgccttctt ttaaaaaaga tgttttgata ttttcaagca cttggtctgc
    22981 tgataacact tttttgcgat gataccaaat cgttgaagct accccactca ataatcctag
    23041 accgatccca acggctaacc cgcctttaaa atagcttaat tcgctttctt catacatgtg
    23101 gatatacctc cttcttctca cttgctactc tttgtgttta ttttaccaca ctctcatatt
    23161 ttctttgaaa taaataaccg ctaccagaaa aaataagcta taataaatac gatcggtctg
    23221 cagaaaaaat catattttat ccgcagatca atataagtct gagaggagtt tagtctgaaa
    23281 tttagacgaa tagcatatgg aagaaaaaac atttcaacga atcaaagagt taacagaatt
    23341 acaaggaaca agcggttttg aacaagacgt tcgcgcctat atggaaagtc atatggaacc
    23401 gctggtagat gagttacagt tagatggttt gggcggcatt tttgggctgc gccaccatga
    23461 agaagcggat gcacctcgtg taatggtcgc agcacatatg gatgaagtcg gattcatgct
    23521 gacacaaatc caagaaaacg ggctttttaa agtcgtacct ctaggcggat ggaaccctta
    23581 cgttgtttct gctcaacgtt ttacacttaa aacctctact ggtaagaatt atccatgtat
    23641 ttcttcttcc gttcccccac atttattacg aggaacaagc ggacaaaaat cagtagaagt
    23701 aacagatatt ttgttcgatg ctgggtttga atcaagagaa gaggcaatga gttacggtgt
    23761 tcttccagga gacacaatcg ttccttacgc tgaaacgatc aaaacagcca acgggaagaa
    23821 tatcatcagc aaatcgtggg acaaccgcta tggctgtaca atggtattag aagctttaga
    23881 agcgttacaa aatgaaacat taggtcatac attgattgca ggcgcgaatg tccaagaaga
    23941 agtaggcttg cgtggttcta aagcttctgt caataaattc aagccagatc tgttctttgc
    24001 agtagactgc tctgcagcag atgacacagt gacgaaaaat ggcacattcg gacatttagg
    24061 agaaggtaca ttgatgcgta tccaagatcc aggtttgatc atgcttcctc gtttgcgtga
    24121 gtacttgcta gatatcgctg aaacaaacaa catcccttat caatactttg tttcaaaagg
    24181 cggtacagat gcaggtgctg ctcatacaca aaatgaagga atcccaagta cagtgattgg
    24241 cgtagtggga cgttatatcc atacgcatca aacgatgttc agtattcgcg attttgaagc
    24301 tgcacgagaa atgttgattc aaacattgaa aggtttagat aaatcaacag tgaacacgat
    24361 cgtatacggg aaataacgcg atagtgaaac tataaaatct ggcaaaagca accttttgtc
    24421 agattttttt tataaaaaga ggatcaatgt ttgttagttc gaaagaaagg tgtataattt
    24481 aattgaaaaa tacataaaca gaaagtagag gaaaaagaat gattatccca aatagtttag
    24541 aagaattagc aggctacgta gaaactggaa aaagtgtatt tttctttact gccggctggt
    24601 gcggagactg ccgttttatc aaaccgcaaa tgcctgaaat cgaaaatgac ttttctgatt
    24661 ggcaatttat tgaagtagac cgtgacaagt acatagatgt agccgctgaa tggaacattt
    24721 ttggtatccc aagttttgtc gtgatccaag acggaaaaga attaggtcgt ctagtgaata
    24781 aagatcgtaa aacaaaacag gaaatcgaat cgttcctaga aagtgtaata aaaggctgac
    24841 atctgttagg aggggtcaag atgaatcaag aatataagaa cattttagta ggtattgacg
    24901 gtagtgaaca agcacttgct gctttcaaaa aagcgttaga agtggctcgc cgtaataatg
    24961 ggaaagtata tgtagcaaac gtaatcgatc agcaatttta taattttatg ggctatgctc
    25021 ctattgatca aaatctagtc gatcagcaaa ctgaaacagc caagcaaatg atcgaagaat
    25081 gtaaagaata tggaaaatct gttgattaca caaatataga aggaattatt gcatacggct
    25141 ctgttcgtga atccatggca gtcaaattgc caaaaaaata tgcaatcgat ctaatcatgg
    25201 ttgggcagtc tggactaaat gcagtcgaac gctttatgac aggaagtgta gccagctacg
    25261 tcatacgtca ttcaccttgt gatgtcttga tcgtttctga cgaagaagaa cagccaagta
    25321 attaagcaag tggaacttgt ataacctttt taatcaaaca gtaaagggac tgtgacaaaa
    25381 gtcttgtcac agtcccttgt tacgaataaa tggtgttcca gaagaagctt cttcggaaat
    25441 aaagcatgaa aaaaatctca tgcagtgaat gcaatttttt gttaagataa atctcagcaa
    25501 aggataatgg aggaaagaac atgatttttg cttataacaa agcacacgtc ggagacactt
    25561 tgatggttat catagccgac gataaaggaa cagaaaacac tgtcgaacgt aaatggaatg
    25621 ttgctcggat caaagatgaa caaggtcaag ttgttgcttg gaactttttc catatctccg
    25681 atcatctaac aattgaaggg aacggccaag taagcttaac agatgaagaa ttagcgaaat
    25741 tgaatcgatt gattaaagaa gcaggattcg aagaacagct tgaagctgat catgaaccga
    25801 agatcgttgt cgggtatgta aaaacatgcg tcccgcatcc tgactctgac catttatcga
    25861 ttactgagac agaagtggac aatgggacag tattgcaaat tgtttgcggt gcaccaaata
    25921 tcgaagctgg acaaaaagtt gtcgttgcaa aaccaggagc catgatgcca gatggaatga
    25981 tgatctggcc aggaactttg cgaggggtag ccagctatgg aatgatttgt tctgctcgtg
    26041 aattgcattt gccaaatgct ccagaaaaaa aaggaatctt agtattgcct gaggatgccg
    26101 tagtcggaga agtatttcca aaataaagag gttgtgacaa gtctttgtca caacctcttt
    26161 attattaatt ttcactattt tgattttgtt ttaatgccga ttgatcgatc gtcaattcaa
    26221 cagtagttgt tttttctttc gagccttcat agtaagtgat tttgacagaa tctcccactt
    26281 ttttcttata caaagcagat tgaagttcta caccagaaga aacttctttg tcatcgatct
    26341 tagtaatgac gtcgtattgt ttcaacccag ctttttctgc cggtgtagct gtttggacat
    26401 ttgtcacgat gacaccgtta gttacagatg aaggaatctt caagatttgt tcttgttgct
    26461 gagtagaaac ggcagataag tcgaccattg tgatacctag agctggacgt gtcacttttc
    26521 cgtctttttc cagttggttg atgatattca ctacatcgtt acttgggatg gcaaatccca
    26581 ttccttctac gcttacatta gaagtcgatt cagaagtact tgcgatctta cttgagttga
    26641 taccgataac ttgtccttcg atattcacta aaggaccacc agaatttccc gggttgatcg
    26701 ctgcatctgt ttggatcgca ttgatattga ctgtttcgtt cgattcattc gtacttgtta
    26761 cttgtcggtt caaggaagaa atgattcctg aagtgacaga attcgcatat tcagatccta
    26821 atggggaacc aatcgcaatc gcaggttcac caaccttcaa agcacttgag tccccaaaag
    26881 aggcaaccgt ttcgactttg tctgaattga tcttcaacac cgctaaatct gaataggcat
    26941 ctgttccaac taattcagct tttaccttcg taccgtcttt catcatgact tccaaacctt
    27001 gctgtccatc tactacgtga ttgtttgtga ctacataagc agtattcccg tcttttttgt
    27061 agattacgcc gcttccttca ctataagctt ccaaattact atcatctgag ctgctacttt
    27121 cttcttgctg cccgaataat ccgccaaaac cgcttgattg gttttgactt tgtaagttga
    27181 taacagaaac gacagcgtct tgaactttat ctaccgcttt ggtgatatca gaatctacat
    27241 tgactttgac attttccaca actgtttcac cggcagaatt ttgattgccg cttgtagcag
    27301 tagaactatt gttgccagtg cccatcgctg cataaaaaat gccagctgtc agtagtcctc
    27361 caacgatccc tccaacaagc cccagaccca acttacgcca gaggctatta tttttatttt
    27421 ttttcatctt tggtgtcaca ttttttctat ccatgtgttc ctcctccaat tacattcatt
    27481 tatctactgt tagtatagtc ttatttgttg actttagtct actttgagac tttgaacttt
    27541 ttatgaaaaa ctttaggaaa aatcattgtt ttcagatgaa aatatttgat tttctttttc
    27601 cacagatatt gtgtaaaact acgcggatag gattggaaaa ataagggata aaacatatcc
    27661 acagcctgtg tataaagtgg ataaattctt ataaaacgta tgtttgccta atgaatgtga
    27721 tgaaataagg taaaacaacg ctgttttctg tggataaacc tgtggatgtt gtgcataact
    27781 gttggggaaa tctttttaac aaaaatagtc aaaataaaca tatagacgaa aaaattgatt
    27841 ataataaaca aagtgagcgt gaagatcaaa ggagagaaaa catgaatatc aaaattattt
    27901 cagtaggaaa attaaaagaa aaatacttga tccaaggtat caatgagtat gtcaaacggc
    27961 tgaatgccta tgcgaaaatc gaattgatcg aagttcctga cgaaaaagcc ccggagaatt
    28021 taagcgaagc gcaaatgaga caagtaaaag aaaaagaagg agaacggatc ttagcgaaaa
    28081 tcaaagaaca agaatacgtc tatggcttag caattgaagg gaaaaatccc acaagcgaat
    28141 cttttgctaa acaaatcgac caattaggta tccaaggaaa aagccacctt gtttttgtta
    28201 tcggcggttc tttaggactc agcgaagccg tcatgaaaag aagcaatgct cagatctctt
    28261 ttggcaaaat gacgtatcca catcaattga tgcgcctgat cctcgtcgag caaatctacc
    28321 gtgccttccg gatcaacgcg ggggctccat accacaaata atgatatttt ttaagagaaa
    28381 aatagcataa aaatctagtt atccgcataa aaactggact tatcacactt tatcaaggtc
    28441 aaaaccactc aatttactac taatttacta cttatgaatg agctttgata cgacgattta
    28501 tccttgaaaa gtgaagatat aaagatactt ccaataaaat ttgaatattt aataggtaga
    28561 cacttcaaaa aatgaggtgt ctattttttt acccgatttt gaaaggaagt gaacttatga
    28621 aaacaaaaaa tcaagaatca aaaggtcgtt ccccactctt taagaccatc aaacattcat
    28681 tcagccaata aaaaagaaag gataggtaaa aatatggaac ttaaatttgt gattcccaac
    28741 atggaaaaaa cattcggcaa tttagaattt gctggcgagg ataaagtcgt tcagcgaaga
    28801 atcaacggac ggctaactgt cttatcaaga agctataatc tctattctga tgttcaaaga
    28861 gcagatgata ttgtggtggt gcttcctgct gaagctggcg aaaaacattt cggctttgag
    28921 gaacgtgtga agttagtcaa tccacgtatt accgcagagg gctacaaaat cggcactcgt
    28981 ggttttacaa attacctttt acatgctgac gacatgataa aagaataaag aaagagagga
    29041 aaaatgatga gattagcaaa tggcattgta ttagataaag acacgacttt tggagaattg
    29101 aaattctctg ctctacgtcg tgaagtgaga atccaaaatg aagacgggtc ggtttcagat
    29161 gaaatcaagg aacgtaccta tgacttaaaa tccaaaggac aaggacgcat gattcaagta
    29221 agtattcctg ccagcgtgcc tttgaaagag tttgattata acgcacgggt ggaacttatc
    29281 aatcccattg cggacaccgt tgctactgcc acctatcaag gagcagatgt tgactggtat
    29341 atcaaggcag acgatattgt gctgacaaag gattctagtt cattcaaagc tcaaccacaa
    29401 gcaaagaaag aaccgacaca agacaaatag tcgctaggta gaaaggagac tttttcgcat
    29461 gaaacagcgt ggtaaaagga ttcgcccatc tggtaaagat ttagtctttc attttacgat
    29521 agcgtcactc ctgcctgttt tcctgctggt tgtcggactg tttcatgtga agacaatcca
    29581 gcagatcaac tggcaggatt ttaacctatc acaagcagat aagattgaca ttccctattt
    29641 aattatcagt ttcagtgtcg caattcttat ctgcttgctg gtagcgtttg tattcaaacg
    29701 ggttcgctat gatacggtta aacaacttta ccaccgtcaa aaactggcaa agatgatact
    29761 tgaaaacaag tggtatgaat ctgaacaggt caaaacagag ggtttcttta aagatagtgc
    29821 tggtcgtaca aaggaaaaga taacctactt ccctaaaatg tattatcgac ttaaaaatgg
    29881 cttgatacag atacgggtgg aaatcacgct gggaaaatat caagaccaac tcttacactt
    29941 ggaaaagaaa ttagagagtg gcttgtactg tgagctgacg gataaagagt taaaggattc
    30001 ctatgtggaa tatactttgc tctatgacac catagccagt cgtatttcta ttgatgaagt
    30061 agaagctaaa gatggtaaac ttcgcttaat gaaaaacgta tggtgggaat atgataagct
    30121 ccctcatatg ttgattgctg gtggtacagg tggcggtaaa acttacttta tactgacact
    30181 gattgaagcc ttgcttcata cagattcaaa actgtatatt cttgacccga aaaatgctga
    30241 ccttgcggac ttaggttctg tgatggcaaa tgtctactat agaaaagaag acttgctttc
    30301 ttgcattgaa acattctatg aagaaatgat gaaacgtagt gaggaaatga agcagatgaa
    30361 gaactataag actggcaaaa attatgctta cttaggtctc ccggcacact tcttaatctt
    30421 tgatgaatac gtcgctttca tggaaatgct gggaacaaaa gaaaacaccg cagttatgaa
    30481 taagctgaaa cagattgtca tgttaggtcg tcaagctggc ttctttctaa tactggcttg
    30541 tcaacgtcca gacgcaaaat atttaggcga cggaatccgt gatca
//
LOCUS       DAFBQH010000029        30453 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.029, whole genome shotgun sequence.
ACCESSION   DAFBQH010000029 DAFBQH010000000
VERSION     DAFBQH010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.029"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..552)
                     /locus_tag="LK694_001402"
     CDS             complement(<1..552)
                     /locus_tag="LK694_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBK5052277.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(678..2606)
                     /locus_tag="LK694_001403"
     CDS             complement(678..2606)
                     /locus_tag="LK694_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBK5052278.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(2912..3628)
                     /locus_tag="LK694_001404"
     CDS             complement(2912..3628)
                     /locus_tag="LK694_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBK5052279.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(3629..5452)
                     /gene="glpO"
                     /locus_tag="LK694_001405"
     CDS             complement(3629..5452)
                     /gene="glpO"
                     /locus_tag="LK694_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBK5052280.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5466..6962)
                     /gene="glpK"
                     /locus_tag="LK694_001406"
     CDS             complement(5466..6962)
                     /gene="glpK"
                     /locus_tag="LK694_001406"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBK5052281.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7098..8558)
                     /locus_tag="LK694_001407"
     CDS             complement(7098..8558)
                     /locus_tag="LK694_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5052282.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSSFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(8548..9843)
                     /locus_tag="LK694_001408"
     CDS             complement(8548..9843)
                     /locus_tag="LK694_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK5052283.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10175..10852)
                     /locus_tag="LK694_001409"
     CDS             complement(10175..10852)
                     /locus_tag="LK694_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBK5052284.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(10882..11963)
                     /locus_tag="LK694_001410"
                     /pseudo
     CDS             complement(10882..11963)
                     /locus_tag="LK694_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
     gene            complement(12064..12498)
                     /locus_tag="LK694_001411"
     CDS             complement(12064..12498)
                     /locus_tag="LK694_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK5052285.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13082..14539)
                     /gene="ascB"
                     /locus_tag="LK694_001412"
     CDS             complement(13082..14539)
                     /gene="ascB"
                     /locus_tag="LK694_001412"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBK5052286.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(14536..16431)
                     /locus_tag="LK694_001413"
     CDS             complement(14536..16431)
                     /locus_tag="LK694_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK5052287.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(16489..18264)
                     /locus_tag="LK694_001414"
     CDS             complement(16489..18264)
                     /locus_tag="LK694_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBK5052288.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18314..19810)
                     /locus_tag="LK694_001415"
     CDS             complement(18314..19810)
                     /locus_tag="LK694_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK5052289.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20004..21626)
                     /locus_tag="LK694_001416"
     CDS             complement(20004..21626)
                     /locus_tag="LK694_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBK5052290.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(21954..23267)
                     /locus_tag="LK694_001417"
     CDS             complement(21954..23267)
                     /locus_tag="LK694_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBK5052291.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(23512..23736)
                     /locus_tag="LK694_001418"
     CDS             complement(23512..23736)
                     /locus_tag="LK694_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052292.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(23756..24178)
                     /locus_tag="LK694_001419"
     CDS             complement(23756..24178)
                     /locus_tag="LK694_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBK5052293.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(24320..25030)
                     /locus_tag="LK694_001420"
     CDS             complement(24320..25030)
                     /locus_tag="LK694_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052294.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25048..25545)
                     /locus_tag="LK694_001421"
     CDS             complement(25048..25545)
                     /locus_tag="LK694_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5052295.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(25582..26034)
                     /locus_tag="LK694_001422"
     CDS             complement(25582..26034)
                     /locus_tag="LK694_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK5052296.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26169..26774)
                     /locus_tag="LK694_001423"
     CDS             complement(26169..26774)
                     /locus_tag="LK694_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBK5052297.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27224..27844)
                     /locus_tag="LK694_001424"
     CDS             complement(27224..27844)
                     /locus_tag="LK694_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBK5052298.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(27846..28715)
                     /locus_tag="LK694_001425"
     CDS             complement(27846..28715)
                     /locus_tag="LK694_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBK5052299.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(28738..29145)
                     /locus_tag="LK694_001426"
     CDS             complement(28738..29145)
                     /locus_tag="LK694_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBK5052300.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29164..30453)
                     /locus_tag="LK694_001427"
     CDS             complement(29164..30453)
                     /locus_tag="LK694_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5052301.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT         8627 a         6625 c         5019 g        10182 t
ORIGIN      
        1 ttttctttga aacatctttc ggtactcgac agcatacaag acaaaagatt gtaaaagacc
       61 gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt ggtaattctg ggaaaagcag
      121 caaaaggatc atgccaacat agatgacagc agtcgctact ttgccatacc acttcgctcc
      181 acttaatttg atatttttag atagcccgat taatccagct acaaacataa agccttcttt
      241 tatcaagaat agaccaaaca acagccaaag ccacggtcga taccatgcca tagacaaaat
      301 cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc accttcccta agtctgttac
      361 ctgattatat ctccgagcaa tgaccccgtc tagataatca gacaaacctg agacaacaat
      421 gaccgctcct gctatataaa atgactgtct cagcacaaaa taaagataaa tcggcagcaa
      481 aaaaatacgg aatatcgata aaagattcgg aatcgtttgt atttctttac gccagtctgt
      541 gcttaatttc attttatcgc cctacctcct cagcactctt ttctttttat tttagcttac
      601 acggggaata aaaccaatga tttcactgtt tttatttttc tttacagcca ctgatattat
      661 gagctaattt gagataatca cttgctgttt tcatcgttta ctcttctttc ttcctgtcgt
      721 ttgcgcattt cttttactag tactttatga gctttttttg atcctcgctc taggtcacgc
      781 tgccgtttac gtgttttata atcgataaac agactgtcta aatcgtaacc tgtcgggtac
      841 agatcttctg ctgaacgcac cagacgaaca cgctgacgag gcacgtggat tatttttttg
      901 tctaaatata catcaatcgt atcttcacct atatcttcat aaactaacgc ttctttttgg
      961 tattcattga caaatatccg atcacctttt gcaaagagct ggcgttcttt tttattctcc
     1021 ttcctatttg taactgctgt cttaggaaga aattcttttt tctgtgtccg ataggctttt
     1081 tccgccaagt aacgctctgc ttgatggatc aatttcatcg acatatccat tttatgagca
     1141 atccacaaag cttggctttc tcctgtttct ccaacccgca actgatattt aggacgaagt
     1201 gcttcccgat cgaaagccat agcagccggc acaaaatctt catgatctcc ggcgaatttc
     1261 ttgatttctc cataatgggt cgtggcaatg atcaatgccc cttgctcata catagattcc
     1321 atgattgcga tcgctagtgc agccccttca tttggctctg ttccactgcc aatctcatct
     1381 aaaagtacca acgtattcct ttttatcttt ttcaaaatag cagcgatgtt ttgcatatga
     1441 ccagaaaatg tactcaacgc attttctaaa ttttggtggt caccgacatc gacgaaaatc
     1501 tcatcaaaga cagcaagctc tgtcccttct tttgccggaa cttgcatacc gaacatcgcc
     1561 atcaaagtca ataaaccgac tgtcttcaac acgactgttt tgcctccggc atttgctccg
     1621 gtgataatca agcctcgata gtctttaccg atttcaacat ccaatggtac cgcatgctca
     1681 ggtaaaaatg gatgtctgcc ttgcttgata atgatatgtt ccgatttatt gattctaggt
     1741 gtgcttccat tgatttctcg gctaaatttt gcccgagcaa agatgatatc taacaccgtg
     1801 atcgtttcga tcaataaatc gatcacttct tcgttttctg cgatagcacc agtcaatgcc
     1861 gccaggactt gatattcttc actgatttct tcagctttta acaactgata gtgttcattc
     1921 aatttagaaa tcacagttgg ttcaataaaa accgtcgttc ttttatttga ttcatcgata
     1981 atcgttccat ctattttatt tttatagctg gctttgatag gaatcgtata gtattcgcct
     2041 ttttgaacaa tcattgcttc ttggatcatt tctttgtttt tcggatgacg caagaatttc
     2101 agtaattttg actggatttc tttttcgatt agttgtaatt gtttgcgtac tttacgcaaa
     2161 ttacgtgacg cgtcgtcact gactttatta ttttttattt tctggtcaat caactcttct
     2221 acatttatca aatcaggaag atgtttgcta taagcaaaca acaagggtgt ctggtattga
     2281 ttcttatcaa aaaactttgt gatcatccga ctgcttctaa gaaaatccgc atattcgatc
     2341 aaatcggttg gctgcagaat caaccctttt ttgacttgtt ctgtcagtgc atcgatcctc
     2401 ggcaacccca taaatggaac atgctgactg ctttctaaga tcaagcgtgc ttctcttgtt
     2461 tcttcttgac gatccaagac agtttgtagg tttgtcgaga ccgtcatatc gctgattcgt
     2521 tttttgctat agtttccaat tgctcttgct tggatttcct ttttgatccg atcaaattgt
     2581 aattgactaa tcatttcgtt gtccatattc tctccttttc ttgaagagat acctgtccca
     2641 aaataaataa gggcctagag ataaataaaa ggatagaaca cagcttaaaa aagtcatgtc
     2701 ctatccttat tagaatacaa agatattgtt ttcctgacag gtaccgtttc ataagcacac
     2761 caaataaact ttgtttattg gctattcgac ttactgatta gataccagct acactcacaa
     2821 aacatacccc attcttctag gtcttattta ttttccactt aactatagca agacacattt
     2881 tggttgtcaa actttagtat gttttataga tttacaccat taatgaatag acacctaccg
     2941 cgataattgc tccgatcatt ggacctgcga ttggtaccca tgaatatgcc caatctgaag
     3001 ttcctttatt agctattggc aataccgcat gtgctaatcg aggaccaaag tcccgagcag
     3061 ggttgattgc atatcctgtt ggccctccta atgaaagacc gatggctaag atcaatccac
     3121 ctacagcgaa tacgttcgtt ccaggtgcaa attcgttttg tccaaaagct agcaacccaa
     3181 gaactaatac aaatgtacca attaattctg tgatcacatt tgctggataa ttccgtatag
     3241 caggacctgt tgcaaacgtt cctaaaatcg cgctttcatc tttcgttgca ttccaatgtg
     3301 gcaaataaga aagccaaaca agaatggccc ctgcaaaagc tcctaaaact tgcgcaataa
     3361 tgaaaggaac gacaagattc caactaattg cccctgtcat cgccatagct agagagactg
     3421 caggattcaa atgagctggg cccatgaatc cagaaatata gacagccatt gttacagcga
     3481 gtccccaacc aaatgcgatg acaatccagc cagaagcctg tgctttactt ttgtttaaat
     3541 tcacagccgc acacacacca tctccaagca aaactaggat cagtgtccca ataaactccc
     3601 ccatgatttg agtcatatca ctgttcattt atttctcccc ctttagttcc cgcagatctg
     3661 attctgcaat caatgcttct aaacgtttag tctgttgcgc cttctcctct tctgtccatt
     3721 caaaatatcc agcaattgca tcgatcacag gttgcttgat ctcatcaagc tgatctcgtt
     3781 caaacaacag atgatttgta cgacgaatga gataatcgcc tggggccaaa accatttctt
     3841 cttctaaacc ataacgtaac cgagctgact cagcgagact caaaccagga taaggtgcca
     3901 tttcttttgc taattcaaag atacgtcggg cattagtccc gtaaaaatca gcgatataag
     3961 tagcatcttc ttcctctaaa ccggctgcta ctccttcttt tgctaattct gtcactgttt
     4021 cttctaattt agttggatcg aagtttccac ctgaaatctg atatttttta gagtcgattt
     4081 ctttcgtctc tattccgtat tcttctttta ataattggcg tatcaatcgc aaagctcctt
     4141 ctgccatctt acggtaatcg gtgattttcc caccagataa agtcagcaaa ccatctggtt
     4201 ctctttctaa agaactacct ctggaaatcg tagaaggtgc tttttcatca cggctgtttt
     4261 ccaaatggtt caacacgtct tctacttcag cacgagaaac tttgttttcc ttatattcac
     4321 ttactgtatc gacaacttta ttgaagctct tgtctgaaat cgatccatta tctccaccat
     4381 tgtaatcaga accagaatta ccaatcaaca gtggacgaag tcctgcccag cttgcttcga
     4441 tatcattcaa tgtgatattt gcttctggat agcgatgatt gatcacatcc aatagataat
     4501 ctacatcttc ttgtgtgact ttcggatccg taaaatctcc ttggtaatcc gtatctgtcg
     4561 taccaaagta agtcttgttt tctcttggaa tagcaaaaac catccgccca tcttgctttc
     4621 ctgtatcgaa gtatgtgggt tgcggtacag gcagtttttt cgcatctaca actaaatgga
     4681 tccctttagt tggacgcatt ttaggagaga ctggacgcgt aaaattcaag tttcgtactt
     4741 tatctaccca aggaccactc gtattgatca ctaattttgc tttgatttcg atcacttcat
     4801 ctgtcagcag gtcacgagct ttgacgccaa cgatttgatc gccatcatat aaaaagccaa
     4861 ccgctttcat tttacttact agataagccc catcttcggc agcctttttg atattatcaa
     4921 tcactaaacg ggcatcattg ttgcggaaat ctagatagac acccgcgcct tttagccctt
     4981 cttttttcaa aaacggttct ctttccaata cttcttctgg tgtcagcgta taattctcat
     5041 atttggttcc tgtcacattt gccagtttgt cataaaggtc cattgctact ttgaccgaga
     5101 acatattgaa ggttgttgct ccttcgccct catagattgg caaaagcatt ggatctggtt
     5161 ttgggatgtg tggggcaata ctttgtacga ctgcacgttc accaactgtg tcagctacta
     5221 cttccacatc aaatgtcttc agataacgaa taccgccatg aacaagtttg gtcgagcgag
     5281 aggaagtccc ttctgcaaaa tcctgcattt cgatcaatcc tgttttgatt cctgatgctg
     5341 ctgactggac tgccacaccg gcaccagtga ttccgccacc gatgatcaac agatccaatt
     5401 cttcttgctg cattttttga atgctatctt gtcttgtctt gtttgaaaac atatggattt
     5461 cctccttatt caaatgcttg ggtagctttt actgcttttt tccaaccgtt gtaaagtttt
     5521 tctcggcgct cttcctccat ttgaacttca aatagcttgc cagcttcata aaattcgcgg
     5581 atctcgtctg tatctttcca ataaccgaca gctaatcctg ctaaaaatgc tgcccctagc
     5641 gccgttgttt ccaaattttc tgctcgttgg atcggtacat tcaagatatc tgtttggaat
     5701 tgcatcagat attcattgtt tgctgcaccg ccatctactt tcaatacagg aatcttgatt
     5761 ccagtgtcat cttgcatcgt atcgatgata tctctcactt gataagcaat cgactgtaaa
     5821 gtagctttaa tgatatcttc ttttgtcgta ccacgtgtca agccaaacat agcccctcgt
     5881 gctgcctgat cccaataagg agcacctagc ccaacgaatg caggaacaac ataaacctca
     5941 tcttcgcttg tagagcgttt cgctgcgtct tctgaatcgc tagctttttg caacatttga
     6001 agtccatctc tcagccattg aatcgaagaa ccagccacaa aaatacttcc ttctaaagca
     6061 tagtaaacct ttccatttat gccgtaacca attgtcgtca ataaattatt tttggataat
     6121 tgcggttttt caccggtatt catgacaata aatgaacctg taccataagt attcttgacc
     6181 atgcctggtt cgaatgccat ttgtccgaat aatgcggctt gctggtctcc tgccatccct
     6241 gaaattggtg cttcgcttcc ataaaaatga taaccttgcg tataaccata aacttctgag
     6301 ttagaagtga cttttggtaa catcactcga ggaatattta gtaaatctaa aatctcctga
     6361 tcccaatcta gctgatggat attgaacaac atcgtccgac tggcattcga gtaatcagta
     6421 acatgtgctt gtccatcggt cagtttccat actaaccaag aatcgatcgt tccaaacatc
     6481 aattcgcctt tttcagctcg ttcttgcgcg ccttctacat gatcaagaat ccagcgtatc
     6541 ttcgttgctg agaaataggc atcgatcact aaccctgttt tttcgtggaa aaattcttga
     6601 tagccttcat tttttaagtt atctgcaatt ccagctgact gtctggattg ccaaacaact
     6661 gcatgataga ttggacgtcc tgtatctttt tcccatatta ctgttgtttc acgctggttc
     6721 gttatgccaa tcccagcaat ctgatttggt ttgatccctg attcgatgaa cgctccggca
     6781 ataactgact gtacagaatt ccagatttca tttgcatcat gttccaccca actttcttgc
     6841 gggaaatatt gcgtgaactc tttttgagaa ctgccgatgt gtcttccttt cttgtcaaag
     6901 ataattgctc gtgagctagt tgtaccttgg tcaatcgcca tgatatacgt tgattctgtc
     6961 attgtgattc cctccattat atgcaaacgc tttctttaac tactcttatc atactactgt
     7021 tttaaaaaaa gaaactgtca aaattttatt ctttttttct ttttttgaca gttttttatt
     7081 atgaagctat tttttaatca attcttttct tttttcagcg tctctattct tcttttcaat
     7141 cgaataatgt cgtaaggtga cgcaaattct gacagataat attcacactg ttttttgttt
     7201 ctctggcaaa atgtgatcac caaatcatat tctttacctt cttcataact tccaatctca
     7261 ataccgccga ttcccctcag ttctctgatc agatattgtt gaaaagcaat acgataaatc
     7321 ggcagacttt ctaaatcgat tccaatcact actggttttg caatataaaa atcgatcatc
     7381 aaaagaacat tggcatactg gatcatcagg taactaagct cactatcttc agctcttttc
     7441 ctcggcaatt gctgcaccgc tgtttcctgt aatttcgcca gtaactgtgt taaggaatgc
     7501 ccgagcattt tctgctgctg ttccaaaagc cgatcacgct catagacttc gatctttcca
     7561 acaaagaaaa accattcatt gttgatctgg gaaatttggt agcctactgc cttttcttct
     7621 ttgattttca aacgacgagg acgataatgc cataacatcg tttcccgaat gtaagtatct
     7681 aaaacagcgg ttggtaactt tttgctccgt gtcaaatcat actgataaaa tatttcttcg
     7741 tctactatcg aaaaagaaac aaaagagctg taaaacatca ttgtttcgta tacattcagt
     7801 tctgctgctg ttcgactaag atgcatcacg atgattggat cgattgcttg ataaagccgg
     7861 tccgactgat agagtaattt cttttctttc aaagttgcgt acgtactttt ttcattcaat
     7921 agtctttttt tcgtaatccc gagccagcaa gcaatctgtg cttctgctga tgccgtaaac
     7981 gttgtttcta atacacggtc caatcctaga atgaacggtc ttttttctgg tgtattttgc
     8041 aaaaaaagcg gtcgctgatc ttccgggata tattggaata gttcataata aaaatatcgt
     8101 atctgtagct cttctccgta taattgaccg tttttgattt gtaatccaaa ttcctctaag
     8161 agctggttca attctttgat ctttcgaaaa acagaagact cactgatatt aaacgctgtc
     8221 gctaatcgaa cgatggatac ttgctgatgt tctaataaat agacgagcat cttaaacttc
     8281 aagctttcct gcaataaaaa caatagaatt ttttccaaac ttacatcttc gtccaacaca
     8341 agcagcgcct gatactcatt tcgctgtact tctacttttt tttccatcat tcggccaatc
     8401 aattcaatat cttccaaata ttgatcaaac gaggcttttg acagttggac atgctctctc
     8461 aaaccattga tagaggctgt tcccccttgc agaacaaggt ttcgcaaaat caggatctgt
     8521 aactgctctt ttctttctaa taatgcttca atcttcatag gcagtattcg ccacctttga
     8581 ccacaccata tctggcacac ggtattctgg ctggaaaaat agctcatgtt cttctaactg
     8641 ttcaaacgtc catccttctt ttactgcaat tgtcagttgg tcaagaaggt ggaacatctc
     8701 ttttttgctt tggagctgtg ctccaagaat ctttttttca gaatcaaata gaaatttgag
     8761 tttaactttg ctttgattta ttgctgtaag cttgtagtct acgtatcctg tttcaatttt
     8821 ttggggataa aaaatgctct cttcttctgt caaacctacg cttcctaaga aatagccaaa
     8881 ccactgattg ccgactggac gataggttcc ctgatccttt gtctgtgagc cactaattgt
     8941 cttgctgaca tttcgtgctg tacggacagc gttatttact agagaaacat acattttttc
     9001 atttgttgga cgaaaagtta cttggatcgc atcgccaatc gcataaacgt ctttttgaga
     9061 cgtttgcaag tagtcatcta cccagatcgt tccatcatca ttcaatgtta attgttcttc
     9121 ccacatctga ttgtcggggc gagaatgatt agccagcaaa acaagatcat agttctcctt
     9181 tcttttttcc gtctctacaa tagcggtttc attttcggtc aaagacacaa cttgttcttc
     9241 taaaaaaagt gaaagccctt gatttttcat ttcctttact aacggttctg tcatttcagg
     9301 atctagataa cgaaaaagaa ttgactttcg gctttcgtaa agatggattt gtttttttct
     9361 tgatgccaac ccttcagcta tttcgattcc tgcttgtcct gcaccaataa ttgcaatttt
     9421 ctttgcattc atgatctttt gctgaagagc cgttacgttt tgtatctttt taactgggtg
     9481 aataagcgtt gattctgcag aaatatttcg aaacttttga tttgaaccag tcgctagaat
     9541 caaacgatca aaatccaatt tttttcctcc tgctaaaacg actttttttt ctttcaatcc
     9601 tattactgtc ttttctgtgt aaacatggat tcgatagcgg tcagtcagtt cttctttcgt
     9661 gatccaagaa agttgttttt cactggtaac ctttcctttt aatatcaaat tgaacccgct
     9721 gggaatccat cctaacttcg attgcttttc aatcaaatat atttcacttg ttggcatcaa
     9781 ttggcgaaga taaatagccg catgaatccc tgcaaacgac ccgccgacaa ttacaatgtt
     9841 catctgcatt cctctttctt ataaggtgtt ctactgctaa cttctcaatc agaaaacaca
     9901 gtattcataa ctcgctttca accttatgat acatagcgat gcagcagaat caaccctctc
     9961 catcataccg tgttctaggt ctgcttctca ataatgtact agtggaccta attgaagagt
    10021 atcatttctg aagagatgtt tcaaaaaatg tactcttata aaagaaaaaa actcgccttt
    10081 tacgtacctc tgtccaaata ggaaacagaa aagaacgaaa agacgagttt tagcgtgtgt
    10141 aatgtaatag ttagaataga cacatcattt atttttaatt ctttaatgct tcaacaactg
    10201 cttcgcctaa ttgatgagca gattgaggat tttgccctgt aagaagccgc ccatcaattt
    10261 gaaccacttc tgaaaaagct gctccagact gatagtttgc tcctttttct ttcaaagcat
    10321 cttctgctaa gaatggtact tcattcgttg taccatttgc ttcctcttct tcattactga
    10381 acccagtcac tgtacgtcca gctaaaatag attttccaga ctcatctgta actggcaaca
    10441 atcctacaac accgtgacaa acagctgaga ccagcccttc atttcgatag atttgtgaag
    10501 cgactttccc aagatctatt gcttctggaa aatcccacat tgttccgtgg ccaccagcat
    10561 aataaaccgc tgcataatca tttgcatcca catcatcagg acgtaaggta tttcccaagg
    10621 cccgatttct gaaatcttca tcttcataat aggcccaatc gatttcatcc atattcaaaa
    10681 cactttgcgg ttctaaaggt acatatccgc ctttaggact gacaaaatct gcatcgatcc
    10741 cattttttac taatacatca taaaaatgag tcagttcact taaccacaaa cctgtaggac
    10801 gctcgatctt ttcgtacttt tctacatttg ttaatacgat caatactttc attttcccat
    10861 cctctttcta taaaaattgc gttattgata acgtgctttc aagaaattat ataatgggct
    10921 tggcaaataa agctctttat atgcttcttc aaaaggtttc gttacaaatt catcctcacg
    10981 gttttccaat aatttctctg cccagtcagg atcgacgatc atttgctgtc caattgctgc
    11041 aagttctgca tatttcaata cctgctctac atcttcgcga ttgcggatcc caccgacacc
    11101 aactaaaggg atctttcctt gaatcgtgtt atggaccact tcaagaatcg atttatcgtt
    11161 gtatttatca gaacgagcta ttcgatcata catatttaat gaaacatgca gataatctag
    11221 ttttgactca cgtaatcgtt caagaagcca gatcgtgtct tcaaaacgga tacctggtgt
    11281 ttcatattct tctggtgaga aacgataccc tacgatgaat ggttttttag catattgttc
    11341 aaccgctgag aaaaccgttg ttaataattc ttcaataaaa cgataacgat tttccaagct
    11401 accgccccat tgatcctctc gtctattcga atgcggagag aaaaattgtt ggatcaaata
    11461 cgtatttgct ccatgcaact cgattccatc aaatcctgat tcaatcgctc tacgtgtggc
    11521 tcttggattg tttcaacgat ttcttcttca ctcattgctc gtggtgtttc cgcatcagga
    11581 cgttttgctg caactgcact tgcagataca ggctgttctc cagataaagt tgctcgactt
    11641 gtcattcgtc caccgtggaa aatctgaata atcgcttttg cgccttctcc ttggattgct
    11701 ttcgctagct cacttaatcg aggaatcatt tcatcatgag cgatgctgag ttcacctggc
    11761 cagccttttc cgatatcttg gacatttgca gcaccagtaa taacagcacc aagaccttta
    11821 gaacgttgtt tataataagt gatctcatct tcggtgatca ctccattgaa aaagctgagt
    11881 tgtgtcgtca taggcgacag catcaatcgg ttcttcaatt gaactcctga aggtaaagta
    11941 attgattgat caaattgtga cattgtcatt cctcccataa ttcgctctga ttaacctatc
    12001 gagaatcatc tatcattaaa ccactcgatt agttgtacat gcaactaatt tgcttaaaaa
    12061 attttatttt ttttgtaact tatctgttgc atcattcatt gtctgggcta attctttact
    12121 tgcttcattt cccaaaactg ctttgtatga ctgatgaagt tcttcccagc taccatcgac
    12181 ttccttagca aattggcgtc cttgttctgt aagataaact aatgacaagc gaccttcgct
    12241 aactgttttg acgtatcctt tatattctaa tttttcaaca aaacgagcaa tcgttgatgg
    12301 ggcggaatag agcatttgtg caatatcttt ttgtggtatt ccatcttttt cttttaatat
    12361 caataaaata aaaccataag ttggtgccag acctgtcctt ctaaacgctt catcagctaa
    12421 tttattcaac atgcgatcta gctttttcac gctaaagaaa agacattcat ctaacgcttt
    12481 ttcaattggc ttttccattt catcccctcc attggcgtac atatcatagt atgattttta
    12541 ttttttgtca aaaaacacgg aataatgtcc gcctcctctg tctagatttg aaaaaaagaa
    12601 gtagccatct tcaaagaatt tcggcttgtt ttcgacaaaa ctccttctgg aacaccgtga
    12661 atcagaggtt gtgaaaaagt cgtttagttt tgagtatgaa gaaaaatttt gtgaaaatgg
    12721 ctttccacat ttttgcataa atttggctta ataccgaaaa actggctttt gaacaccgtt
    12781 tatatcagag gttgtgaaaa agccgtcctg catcaagtaa taagaacagc caaacaaaaa
    12841 tagttcctca tattttgtcg ttttttgagc taatgccgat ggtcagctga ttgtacaccg
    12901 tttattgaga ggtcgtgagc ctgccgttga actccgagtc acaaggagca atttcaaaaa
    12961 acagcttctc atgttttatg actatggtga cttgtgatcg aggagttggc aggagaatac
    13021 tgtttattcg taaagaaggg gctgtgacaa gacttttgtc acagtccctt atttcctttt
    13081 attataggat ttcgccattt gattggatga cttgtctgta ccaatcaaat gattttttct
    13141 ttaaccgttt ctttgtgcca ttcccttcgt catcttgatc aacatagata tatccataac
    13201 gttttgacat ttgtgaagta gaagcgctga cgatatcaat gcagccccac gacgtataac
    13261 caaataaatc tactccatct ttgatagctt ccttcatctg ctcaatatgc tgcttcaaat
    13321 agtcaatgcg gtaatcgtct tgaacggttc catcttcttc tactttgtct atagctccga
    13381 gtccgttttc agctatgaac agtggttttt gatacctatc atataattga ttcaaagcaa
    13441 ttctcaaacc cttaggatca atctgccagc cccattcact tgatggaaga tacggatttt
    13501 taattcctcc agttattaag tttccttgga ccccttccca attttctgga tgagcactga
    13561 ctgcactaga catgtagtag ctaaaagaaa caaaatcaac agggtaggct gctaagattt
    13621 cttcatcacc tacttctatt tttaagttga cacctttttc ttcaaacatt cgttgcgtat
    13681 acgctggata atatcctctt gcttgaacat cactaaagaa caaagaagtt ctttgctgct
    13741 caattgtttt ttgaatatca tccggatcac aagaatacgg aacaagaagc tgatagctga
    13801 tcatacaacc gacttgtgaa cctttgataa tttcatgaca agcttttgta gctaaagcac
    13861 ttgctataaa ctgatggtgt gcaccttggt atttcgcttg ttccaaatgg ggatggtttt
    13921 cttcaattac tcctacgcta acaaaagggt gatgttttac acagttgatc tcattgaaag
    13981 tcatccaata ttttacttta tttttatagc gtttgaatac tgtttcagcg aaacgaacgt
    14041 aaaactgaat aagctttcga ttaggccatc caccatattc tgtaaccaaa tataacggca
    14101 tttcataatg ggaaagagaa acgactggct caatcccata ttttagacat tcgtcaaaca
    14161 cttgatcata aaaaaataat cccgcttcat tgggtgtttc ttcatcacca tgaggaaaaa
    14221 ttcttgtcca tgcgatagac atccgatagc atttaaaacc catttcagca aataaacgga
    14281 tgtcttcttt aaagtgatga tagaaatcat ttccgtgtct tttaggataa taatacttgc
    14341 tgttgggatc ctgcgccgct tttacttgct gatgcgtcat catatgccat tgatctgtcc
    14401 atttttcttt aggtaaatcg ctgtcgaatg tatacacatc agacacagat aatccttttt
    14461 catctacatc ccatgcacct tcacattgat tggccgctgt cgctccgccc cataaaaatc
    14521 cttctggaaa ttttttcata tcgatccccc taaacttgct agttttcttg tttgattatt
    14581 tttcttatga ctagtaatga tatttttgaa tctactacta tttgtaacaa taaccattgt
    14641 ggtcggatca tatccctctt tgattaattc ttgcaaatct acttccatca ataaatcgcc
    14701 tttacgtact ttttttccat cagatactgc actttgaaag tatttgccat ttaaattcac
    14761 tgtatcaata ccgatatgga tcagtatctc tgctccttct tcactgacta ccccaattgc
    14821 atgtttcgtt ggaaaaactg ttgaaacttc cccgtttaca gaactgtaga tttttccgtc
    14881 cgttggaatg atcgcaaacc cttctcctaa agatccacta gcaaatgttg gatcgtttac
    14941 ttcagataaa tcaattattt cacctgctgc aggagtactt aacactatat cctttgagga
    15001 ttcttcttcg atttcatcct tgtagccgaa aagaaaagtt agcatacagg aactgatcat
    15061 cgctaagaaa taactagcaa ctagccaaac aaatccttca ccaatataaa tcgggaaaga
    15121 caaaatactt cttgtagcaa ctgccaaagc accggctcct gcggcaccag ctactccacc
    15181 aaatatagct ccacatatac aagcaatgta aaatggtcgt ttatatttaa gagtcacacc
    15241 ataaacagca ggttcagtga ttccaaataa tgctgtgata aatgcagata aagataaact
    15301 ttttactttc ttatttttcg ttttcaaaaa taccccaagt actgctcctg cttgcgcagc
    15361 tacggcagtc atacatgctg gacctagcgt atcacgaccg tagaaagaaa gattgttggt
    15421 cattactggt acaagtcccc aatgtaaccc aaaaatcact aacacttgcc aaaatccgcc
    15481 gataacagca ccagataaaa cagggctaaa gttataaaca gctaaatatc cagctgcaat
    15541 cagctgactt gcataatctg caattggacc tacaactaat aaaataattg ggaccatcac
    15601 gatgatacaa atgaatggtg tcaataaatt tcgaatactt tcgtgcaagc ttttgcgaat
    15661 cttaggctca atataagaaa gaacccatac agctaaaatt gctggaataa ccgtacttgt
    15721 ataccttgct agaatcacgg gcatccctag aaatcgcaag gttatgctat cactatatgc
    15781 actgacaatc gtaggataaa cgagagctgc tgcaataacc atagcgatga aacgatctgt
    15841 attgaacttt tttgcagcag tatatgccaa aaatataggc aagaaataga aaaatgcatc
    15901 agctgctgca ttcaaaatga tataagttcc tgattcagta gttaataaat tcaaactcgt
    15961 acataaggcg agtactcctt tgatcaaacc acttcctgcc aagacgccta aagtcggggc
    16021 aaaaacagcg gcgatcatgt ctataaaaga gtttaaaatg ccttttttct cagttcctct
    16081 ctttccacta gatgcatcat cttctagttt catttgtttg gataatacgt catacacttc
    16141 atttactgca tttcctacta cgacttgaaa cattcctcca gcttcgacta cagtgatcac
    16201 accttctagt tgctctacct tctccttatc tgccttctta ttatctttta gggtaaagcg
    16261 taaccttgtt gcacaatggt aaacattgct gacattttct tcgcctccga caaatcggac
    16321 aatatctttc gctaattgtg aatagtccat ttttacttcc cccttgttaa ttaatgccgt
    16381 agcaatcctt cttacaagat aagtatagtt cttagaaaac ggtttctcca ttcaattagt
    16441 accccttcta atagcgaacg aaactgtaca atagtgtgta tggtttactt agccgagcaa
    16501 atcactcact gcttgaaaga aaacaagtat atcgtttctc ttatagtcaa aggagacaaa
    16561 aaaaacacgc ttgatcttcc tttgctgatg tataagtggt atctcaagct gatactctgt
    16621 gatttttgtt tctacttggc ttttgataag acagcacaac aaagtctcgg catttaacgg
    16681 aagcataact aggtcagaac taggagagat tagttgctga atggaatcta tctttttatg
    16741 gatttttatt tgattttctt ctttgtagta atgatcaagc agttttgttt tctttttttc
    16801 ttcaactatg gtcagctttt cacgcaaaat ctgataaatc tcccaagatt caaactcatc
    16861 tagaacacta ggtagataaa tcaaatcatc ccactcaaaa ataagctctt gctcagtagt
    16921 caaaattgca tcatattccg ttattttgtt tgattgattt ttgaaaagat acactggttc
    16981 tatccggcat tctttgatcc acatgttcat actttgttga atctgttttt ttagtgtatt
    17041 caaaatacta attggctggt tactaacaat cagtatctcc ccttgttttc gaaaaggatt
    17101 caacgcttcg gcaagataca aaacaaaata agcgctttca gctaaattgg gacgtaattg
    17161 aaaatgtatt gggaagaaac gccgaattaa ataaatttct aaaggataac gggagatgat
    17221 cttttgtaca aaatgattca tggtattatg tccggcatcc atcctcgttt tcatttgttt
    17281 gatatgggtt agcaaaaatt ctgtttcatg gaacaataac gaaacaggaa gatctaaaaa
    17341 acgaataatc gtttgctcaa atgctcttag attcttctct atttctttgt ttttttcatc
    17401 attaatcatc aaataattta actctaatag tcgattttca attgcttgtt tttcttgatt
    17461 ggaaaaagaa tagggtagtt ctgatgataa cttttttgtg agggtctcta ccatcggaat
    17521 aaataatgta tacctgtttg ttttttctaa aagttcttct cttcgaagaa tggtcaaaac
    17581 gagataccga gaaaatcgta tgaagtcctc tcctgaaaca atataattat atgatataag
    17641 aacctctttt aatgtctttt ggacagtcgg catgatattg gaaaaaattg tactgatttg
    17701 ttgaggaaac cttgcgtgtt ggatcaccgt ttctgttcca acaagagata agaaaattct
    17761 ttttgaattt tcttcagcaa tgatttttag tccttttgtc gacgaaactt ccagttctaa
    17821 tttaggattg cgctcgatcc aacgcaaaat cgtttttatt tgtaatgata cggctgtttt
    17881 tgaaagaaag aaaatgtctg ctatcttttg catactgaca tacccttttt gaaacaataa
    17941 gtacgtcaca atagttgaag ctcggaaatc catgctggaa tagattttat gtttcgtcac
    18001 ctcttgtatg atttgctgtt tagtttcaga ttcaattgat tctaaaaaat aacctgaacg
    18061 tttatttgac tggataatga ttttcttctc gtagatagca agcatatttt tgatttcagt
    18121 ttggatcgtt ttcttgttgg tacctaaagc ttcagctaat tcttcgcttg ttatccaatg
    18181 attcttattt cttaatacta caagaatttg gagttgtcgt ttatttaagt ccatttgctt
    18241 acacctcctc tttgttataa acatacagga aaaacctctc aaatgacaat agtcacttgc
    18301 agaggtttaa aaatcatttg aagaaagatc tatattcaag gtttttacca ttagactgga
    18361 taatttgttt ataatattca aaactatctt ttttcaatct cctaaagctt ccgctcccat
    18421 cattttgtct atccacataa ataaagccat atcgcttcct catttcccct gttcctgccg
    18481 atacgatatc gattggtccc caccataagt aaccaatgat ttggcaacca tcttttattg
    18541 cctcagcaat ttcctgaaga tgtattttta gatactcaca acgttttgga tcgtggattc
    18601 gattcaattc atctggtgct tcatctagtc caatgccatt ttcaactatg aataatggta
    18661 attcataacg gtctgtcaat tcattcatga cataacgaaa ccctagagga tcaattggcc
    18721 aactccaagg ttcgggagat ttttccttca aaaagggatt atcgaaacca tttgctccgc
    18781 cagtatctac gcgcatttct gcttcttttt tatagacagc gctacgataa taactaaatg
    18841 caatataatc tgccgtgtta gcttgcaagc actgttcgtc tccatctaac attactggtg
    18901 cacagtcttc ttccttccat gtcctgtaaa tataagctgg ataatgccct cggcacatga
    18961 catcggagaa aaaccatgaa ttgcgtttaa attgcagtga accaaataca ttctctggat
    19021 cacagtcata tgcataagtc gctaatgcag aaaagctaca cataataccc atcttagcat
    19081 ttggatcgat tttctttaat aattgagtag cacgggcgtt cgctagaaac atatgatgac
    19141 aggcttgata aatcactttt tttgttagtg tagcaatggg ttcaagttta tctctagcag
    19201 ggaatgtaac caatccagca gctgcataag gcatacgaaa agcattattg atctcattaa
    19261 aggtcatcca atatttcact ttcccttggt agcgttcaaa gacggtctgc gcataaaatt
    19321 caaaaaagtc gattaatttt cgattttgcc accccccata ctctcctacc aatgctaagg
    19381 gggtttcata atgactaaga gtaattactg gctcgatatc atatcgattt aatgtattga
    19441 ttagtctgtc ataaaatacg agaccagcct catttggctt ctgttcgtct cctcttggaa
    19501 atattcgtgc ccatgagatg cttgttcgaa aagcttttaa acctaactcc ttcagtaaac
    19561 gaatatcctc ttcaaatcga tgataaaaat caatcgcttc atgagaaaga taatggagtg
    19621 attcgtctaa agcaatctcc caaatctttt tttcttcatt ccactgaata ctaggatgct
    19681 ggttcatgat cccgataagc gtatctgtca cattaggtaa tttgccgtct tccaaccatg
    19741 caccttcaca ttgattagca gctactgctc cgccccataa aaaattttct ggaaatattt
    19801 cctgatccat aactatctct cctcgctatc aacaatttct tacttgaagt atagctcgat
    19861 tcatcaaaac aagatattct aactatttcg gcagaagaga agaaaaaaat tgaatgctac
    19921 tcttcatttt taatatttat atataacaaa tacataagac gatccccgaa agaaaacact
    19981 cgttaaccat ctaagcaaac gatttatttt acttccaaca caaatgcttc ataaggtcgt
    20041 aaatcaactt tgccgtctgg taactgtttc agaggataat ttgacacaat aacccttaaa
    20101 gcttcacgtg gaatagtgaa cgtctgcttt tcactggaca tattacaaat aacgagccat
    20161 tgctggtctt catatatgcg agaataggca aagacatgct caggcgtctt ttccactaat
    20221 tcatagtctc cccagacgat gatgtcatgt tttctacgca aatcgatcaa tttcttgtac
    20281 atataaaaaa cagaatcttg atctgctaac gcttcttctg cattgatttc tttataattt
    20341 ggattgactc gaagccatgg aattccagta gtaaacccag catgttcacg ggcgttccat
    20401 tgcataggcg tcctagcatt gtctcttcct ttcgccttga ttgattccaa gatttcctct
    20461 tttgtaaatc cagaagaaat cctttcgtta tacatattga ttgtctcaat atcttttgct
    20521 tccgcgatat catcgattgg gcaattcgtc atgcctaact cttctccttg atagatataa
    20581 ggggtgcctt tcatcatatg gagcaagata gcaaacatct tggcactttc ttttcgatac
    20641 acatcgtcat ttccccaacg agaaacaatt cgcggaagat cgtgattatt ccaaaataaa
    20701 ctattccatc cttcatcacc taaagatgtt tgccatttgg ataatacttt tttcagttca
    20761 gagatactca agggtttcaa atcccatttg tctttgcctt cttgctgatc taatcctatg
    20821 tgttcgaatt ggaagaccat ggataattca tttcttttag gattggaata aagtttagcg
    20881 atttcggtag ttgctcccca tgtttcgcca acagtcatca gatttttgtc accaaaagtt
    20941 gcttgattca tttcctgaag atactcatgc agtttaggtc cattacttgt gatttcttta
    21001 tccggttctt tgccaatcat atcgatcacg tccatacgaa agcccccaat acctttatcg
    21061 atccagaaat tcatcatttg ataaatctct tggcgaacct gtttattttt ccaattcaaa
    21121 tcaggttgac gcttgctata caagtgcaga aaatattgcc ccgttttttc atccaactgc
    21181 caagcagaac cactgaatat tgagcgcaat ctattaggct cttttccatt gaccggatca
    21241 cgccaaatat aatagtcccg ataaggattt tctttatttt tacgggcctc aatgaaccac
    21301 ggatgttcat cagaagtatg attcaccacc agatccatga tgatacggat atttcttttc
    21361 tttgcttctt caatcagttc ttccatttct tccatcgtac cgaattctgc catgattgct
    21421 tgataattac tgatatcata cccattgtca tcattcggag actcgtatac tggagaaagc
    21481 caaattgcat caatacctaa atacgccaga taatccaacc tggaaataat cccccgcaaa
    21541 tctccaatac catcttgatt tgaatcttga aaacttcttg gatagatttg ataaacaact
    21601 gaatggtgcc accatttttg ttccatgatt ttcctcctat aaatggatgt ttctattttt
    21661 tgcttaatca tttattttag tctctctcac taaaaattgt aacgctttca aatagctatt
    21721 gcaccttgtt atcggtaaca gaataaaaca aaaaaacagc tgaggaaatt ccctcaactg
    21781 ttttttgaaa ggattgattt atgcagggaa gaatgctgat ttcacaactt ctttataaac
    21841 ggtgttcact cagctgtccc ttggtattag ctcaaaaaac ggcaaaatat aagaaactat
    21901 tttttcgttt ttttctctaa tactcaggcc agaacgctga tttcacaacc tctttatttc
    21961 acattcaata tccacatgaa tcccacaaat acaaagaaca gaccatacat gattggagat
    22021 acttcttttc ctcttttagc tgctaacatg gtaattggat agaagataaa acctaatgca
    22081 atcccatctg aaatactata agtcaaaggc atgccgatca agatcaagaa tgaagggatc
    22141 gcaatctcta agtttcccca ttcgatttgt cgcaaagatt gtgccatcaa tactcctacc
    22201 ataatcaatg ctggtgcagt tacttgtgaa gtaacaactg atagtaatgg agaaaagaac
    22261 aagcctaaca agaagaaaat ccctgtagta atagctgtga tacctgaacg tcctccaacg
    22321 gcgataccag ctgatgattc tacaaatgca ccaacaggag aagtaccaaa taaggaaccc
    22381 gcaagcatag ctgttgaatc agaagctaaa gctttcccta cacgaggcat cttgttgtct
    22441 ttcatgaaac ctgcttggtt tgctaatcca actaaagttc ctgctgtatc aaagaaagtc
    22501 acaagcaaga atgtcagcac tacgacccac atttgaagag aattgatgtc ccctacatgt
    22561 tttaatgcaa ctaaaaatgt tggtttcaaa cttggtgctg cggcaacaat ttcagaaggc
    22621 attggaatca aacctgtgac cagacctaaa atcgttgtag ctgccatccc gatgaagatg
    22681 ccacctggta cacgccgaac aagcaagata gcagttacga ctaatccaaa aatcgtcaac
    22741 caagtagaac caacgttgag tgatcctaat cctactaatg tagattcatt cgaaacgatg
    22801 atcccacctt cgctcagacc aaggaaagcg ataaatagac cgataccgcc agaaatcgca
    22861 tatttcaaat ctgctgggat cgcatcaatg atcatttccc gcagcttgaa gattgtgatc
    22921 aaaataaaaa tcaaagaagc aacgaatacg ccggctaatg ctgtttccca tggaatcccc
    22981 attcctaaac aaacagaata agcaaaaaaa gcattgatcc caagagcagg tgccgttcca
    23041 attggataac gggctaatac ccccattaaa acacagccga tcgcactggc taatgcagtc
    23101 gcagtaaaca ctgctccttc atccattcca gaagcaccta atacagttgg atttacaaat
    23161 aagatataag ccattgagat aaatgttgta aatcctgcca gtacctcacg ttttaccgtg
    23221 gtgttcaatt tttctatttc gaagtaggaa agtatttttt ccttcatctt ccatccccct
    23281 aaatccgaac attataaata aagtccgtta aaatttcgat ttattaataa aacaacgtgt
    23341 tttatcatat actaaaacaa aacataagta aacaaaaaat tcggatttta agcaaaatcc
    23401 gaacgttcca tctgattaaa tccctttatc tcaaagttca tgaacattca ctttggatta
    23461 caagaatttc tttattgtgt taataaagtt tcttcattct agtctcaaaa atcaatagtg
    23521 tgatttgatt ttaccatttc gtttctgttc ttctttgatg aattcttttg tatcgacagg
    23581 attcagatta tttagtccgc caaaacctgt atcgaccatt tttccccagc cttttgtctt
    23641 cttttttcct gatttgagat tctttgctcg aagcttctca ctatgttttg ctcgccgata
    23701 cttcaaataa ccttctaatc ttcccattag atccactgtt tttcctcctt tttattcaat
    23761 cagacgactc agcttttcct ataacaaaat aattgccttc agggtcagaa aaattgaaag
    23821 tcaagatgcc attgttatct gtgatttctc ctggttcttc catgttctcg tagatagttt
    23881 caaattcatc cgtaaaaaat actaaagaag gcggtggtcc taatacctct ggtgaaaact
    23941 tctgcacaaa tacttttggg aatacgccta attctaattc ttttgagatg ctgagtacaa
    24001 tactcttaaa ttcttctggc aattcgattg tttcagaaat ttctgcacca attttttcta
    24061 caaagaatcg gcaaatcatc tctacgtcat tgacataaag catcacacgt gttttttgta
    24121 tcatcatttg acccttcttt ctatttgatt ttctttcctg tctataaact gattccatta
    24181 tatcacgaaa gcgcaaacac cttgtgtttg atactattca agaaaaataa agcaatagtt
    24241 tctaggattg ctttcctcca caaacttttt ttacaaaaaa aaaacggcaa tgaatgccgc
    24301 tcttcttatt tcgattcttt tataattctg cttctgattc atctttggtt agtgtgtgtc
    24361 taacactagt tacgccaaat gtttgatcga ttttttcctt caacgcttcg attgcctgtg
    24421 cgccacgtgc cgcttgttca gcattgatgg attcaatgac cagcaccgct gcttgtgttt
    24481 cttccgtcag catcgttctt tgggcttcac gccagttgag acagcgacag atcgctcctt
    24541 gttcatcata gtagatgatt tcttccggaa gtgccggagc atcggaatct gcacctaaag
    24601 gcaggaatga ttcgccacct tgggcttttc ctaaatgcag atttcctgcg atcttttcga
    24661 tatcttctcc gccgcaaggt acgccaaaag acaaggaaat actattatag atatctacaa
    24721 gcggattgat tgggaaaaat tcccgttctt gatttgcacg tttcagtaaa gcttcaatag
    24781 aagaacgtgc acctttttta gtcttgaatg tggtaaatgc ctgacgccac tgagcaatga
    24841 cttcgttttg gctgaatgtt tcttctgtaa ggtaatttct tgcttctttt gctgcctcat
    24901 tcaataattg atgatacgtt tcttcacttt cttttgttac gtggttatcg ataccattta
    24961 cggataagat ttcgatggtt gcttcaggaa atacttccca gaaattggta tccactatta
    25021 cttttttcat ctgttcacac ctcttgatta gaataattgc ttgatgaata gatagtctgt
    25081 ttggggatca tctcccatat aaaaaacatg ttcgctgtat ttgataaatc cttttttacg
    25141 ataaaaagcg agcgcaacat cgttgtgttc ccaaacaccg agccaaatgg ctgttttttg
    25201 cttttctttt gctttttcaa tagctgtatc tagtaaaaat gtaccgatac cttgatgttt
    25261 aaattttttt cgtacataga ttcgctcgat ttctaaaaaa tgatcagcca tttcctcaga
    25321 ttgactcgtt cctgtattta gtttcaggta acccgcaggc tcttgatcga tactcactag
    25381 gaaaaaatcg gtttctttat cttttagctc ttctgtaagt ttttctagtg cataagcttg
    25441 tgtcaagtaa tcagctaaat gctccggtga attatctttt ccaaatgtat ctgtaaatgt
    25501 ttcgatacta atttgttgta gtaagtctac atctgaaggc gtcatttttc gaatttcgta
    25561 tttattcacg gcttcttcct ttcaatagat ccttttattg ccttttttga caaaattcca
    25621 atcttcgcta acattcttgc atactttatt taaaagatat tccaattgtt ttgcttcttc
    25681 gtcagaaaga ccatttaatg cgactgcatt ggagtagttg ttttctcgaa tgatgaaagg
    25741 atagatttct gctccttttt tagtcggata gagtttcttg atttttttat tttctttatc
    25801 ttccaaacgt tcgatcatcc cattggattc taatttcttg attgctcggg cagtcgttgt
    25861 acgatctact ttgatcattt ctgctagttt ttcttggatg attccaggat tttcgcaaat
    25921 acgtactaaa taaagatact gtcctcttgt taaatcgact tctttgaatt cgatattgct
    25981 aatagaatct agcgctcggg caatcattcc gatgtctcga aggatttcag ccatttcatc
    26041 ccacctttat tttgtttgta agactagttc acctcttttt gttgcaaatg caactatttt
    26101 caataaaaaa atcaagttct tttttaataa ctagtaaagt atattctttt cttcttttac
    26161 gaaaaagctc aatcacaata gatctggttt ttgttttcta aaataagtaa ctgtttcttc
    26221 gttgttcgct tttctgatag ctttggcaac tcttctatac taaaaaatcc catgttggaa
    26281 gtttcattat tctcaataaa cttggcctct tcaccgtgga tcgcacaagc aaaaacaagt
    26341 ttataatact gaaaaagctg tggaatatct ttttgtttat tcgtatcaaa aactgctcgc
    26401 aactgagtag tttctactgt taatcctgtt tcttcataga cttctttacg cacattttct
    26461 tcaggagaca atcctatttc agcaaatcca ccaggaagtg accattcttt cgtccgttta
    26521 tcttcgatta gtaggacctt gccatctttt ttgatatagg cacgaacatc tacttttggt
    26581 gttggatgtc cttctccaat cgttgtgagc gcttttattt cttcgaaagt ttcttttcct
    26641 atttcactga tcagttccaa tgaaatcgtc cggatctcct caaaacgctc tttatcaaat
    26701 acatcttttc cataatatag accagcttga gaaagtgcga gtagtctttt gtattgagcc
    26761 aacttcttgt tcattctctt cccttctttt ttagcaaatg tatgactatg taatcaagta
    26821 tagaagactg gtttaattac ggcaaataaa tataatgaaa tctgcaataa ataaaagaat
    26881 gttaaaaaac tcgagaacaa aagacattcg acttctctgg ggctgtgaat aaatggcgtt
    26941 caaaagcttg catctgcggt aataagcccc accaaacgaa aaatatgacg agctattctt
    27001 gtctggctgt tcttattaat tgaggcagga cagctttttc acaacctccc cactaaacgg
    27061 tgttctccta ccaactcctc ggtcacaagt cacaatattc atcaaacatg agaagctgtt
    27121 tttttgaaat tgctccttgt gactcggagc tgaacgacag gctcacgacc tctcggtaaa
    27181 cagcgttcaa aagccaattt ttcgatatta agccatttct ttgttactta ttgtcattga
    27241 tttctttttc taatttatcc aatagtggag tgtaatccac ttgagtatag tctgttacat
    27301 caaattcata tagtgttcct aactggaatt tgtcatattt tcggtcggcg atgatctttt
    27361 tgaatgcttc tttcgtaata tgattggtcc ctttcgttcg atcttccaat acgtcaccat
    27421 agtgataatg atccatgata tagctcaaat gacgatctga ttcacgatca cgtttgtacc
    27481 gacgttgcca taacacttca aagtctgctg ttaggcgaat cgtgatcacc tgatatccgt
    27541 atctatctgc taattcttgg agtcgtgctt tttgcttatc actaaaagga tactcggaca
    27601 caatcacttt ctttcctgct tccatataca aatcaagtgc tttgtaatag aaaggataaa
    27661 ctttcgtcct ttcttgttcc gcccgttctt ccaaattgtt aaacccgatg gattctgcat
    27721 agtattcctt gatttcatcg agcgtgatgg catacatatc tggaaatcgt tcccgtagtt
    27781 tattcataag atatgttttt cctgtaccag gactaccagc cagaagaatg aaatactttt
    27841 tcatctcatt tatttcctaa gttaattgga ctttccaagt tcataaacag tccaatctta
    27901 tgcttgacaa tttcttttac tgcttcattt gctggaatca cattatggcg aagagacttg
    27961 ttgatttctg tttcactaaa ggtttcttta gctactttcg tccagttgac taaaagctcc
    28021 gttccaacat tgaatttgcg gatcccgtta ttgatcaatt caggataatc ttcttctttc
    28081 acacctgttc ctccatggat aactaggggg acatcaacta ctgcatcgat ttctttcagc
    28141 aagggaatgt ttacttctgt ttttgatttg aattgtccat gattcgtacc gatagcaata
    28201 gctaatgcat ccacgcctgt agcttctaca aatttcactg cgtcttccgg tcttgtatat
    28261 accttgtcat tttcagcgac atggattcct tcttctgtac cgccgattgt tccaagttct
    28321 ccttctacgg aaacaccgtg cgcatgggca tattctacta ctgcttttgt cttcaggata
    28381 ttttctttga aaggcagatg ggacccatca tacatgacag aagtatatcc gctgtcgatt
    28441 gcttctttga tatcatcaaa gtctcgggcg tggtctaagt gcaagactgc atcaaccatt
    28501 tcttcttctg caatagctgt cattacattg actaacacat gcatgccgat atatttagcg
    28561 gtatcgacac ttgtttgaat aatgatcggc gaacccattt cttttgctgc gcctagcata
    28621 gcaggaagca tttctaggtt atgcgtatta aaagctccaa ctgtaaaatt caactcttct
    28681 gctgtttttg ttacttcttt caatgttgta tacatctttt attgccacct tatcttttta
    28741 tttgtttcct ttgatcgtta cttgttttgc aatttgtcgc atctcatcca ttttgcccat
    28801 gtagtaatcg atcccttgat catcaccagc acgagcagca tctgcacgct tctggttata
    28861 gaggctcatc agttttttat acccatctcc gatcgtctgt ttattttcca aactcttttc
    28921 taaatattgg atggcttgat cgttcacata caattcagaa aatttaagtc caacttgttc
    28981 ataaagtttc gctaatttat gcttatcttg ggtagtggaa atctcttgtt tcaattgtgt
    29041 aatctctgat tgaatctttt cggtcgcttc tggcgatagg gctttttctt cttttgccag
    29101 aacttttttc ggttcttttt cttttttctt aaaaaatcca aacatcattt tccctacttt
    29161 caattaaaat gctttcaaaa ctgggcttaa gatccaggca tataagagtg ctgcggctac
    29221 tgtatctaca tcggttgctg tagcattgtt gaatcccatc gaattaaaga tcgttactag
    29281 caaagcgggc aataatgtaa taaagaaacc atgagcaata ccgccaatga tcgctcctcg
    29341 gcgtccgcca actgcgttgc caaatatccc tgctgtccct ccggcaaaga aattggttag
    29401 cataccaggt aaaatcatcg ctaagccgaa catgggcaaa gtaaacatgg caataatcgt
    29461 accaatcgtt gtcgtaatga aacctaaaat gacagcattt gggctataag gaaagaaaac
    29521 tggacaatct aaggcaggga tcgcatcagg aactaatttc atagctatcc ctcgaaatgc
    29581 cggcacgatt tcacctaaca gtagacgtac accagctagc aatacataca cacccactac
    29641 gaattggatg gcttgtaaaa aggcaaacat gatatagttt tgatctcctg ataattcaga
    29701 agcgaatggt tcgcctgcaa ataaaacagt gatgatatat agcgggacca tcacgaccat
    29761 gacagacaaa taagtatctt gcagaaattc gaaatgtgtc ggcaacttga tatcttcgat
    29821 cgacttttta ttttcgcctt tctcaccaaa aagcttagca acaccggctt caaataagta
    29881 cccgatcgta caaaaatgac ctaaagcaat atcatttgat ccagtgattt tacgaatgat
    29941 cggttgcgct actgccggca tagcaatggc gaaacaagct ccgataaaac caccgaccaa
    30001 gatcaatacg atccctcgca gaccggcaaa atagccaaag actgtcgtca tcgttgccat
    30061 ccataaaatt gcttgaccag ttaaaaaaat gtacttccat tttgtgaaac gagccaaaat
    30121 aatattgaat acaaaaatgg ctaagaacgt cagtgcaata tctctaccta aacccaaatc
    30181 attcattgct tgaccattga tagattcaat agaagggatg atcccttgca tatgaaatcc
    30241 ttcagtaaag attttcccga aataagttaa acttccaacg atgatgctcg aaccggcact
    30301 caacacttga aatcctagga gtgtctttaa cgtgccagag atgacttgtc ctgctgattt
    30361 cttctgcagt agtagaccca acatagcaat caatgcaatg gtgatcgatg cctgagtcaa
    30421 aatattttcg atgataaaat tgataacgcc cat
//
LOCUS       DAFBQH010000030        27653 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.030, whole genome shotgun sequence.
ACCESSION   DAFBQH010000030 DAFBQH010000000
VERSION     DAFBQH010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.030"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(308..670)
                     /locus_tag="LK694_001428"
     CDS             complement(308..670)
                     /locus_tag="LK694_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052302.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(744..1124)
                     /locus_tag="LK694_001429"
     CDS             complement(744..1124)
                     /locus_tag="LK694_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBK5052303.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1188..2381
                     /locus_tag="LK694_001430"
     CDS             1188..2381
                     /locus_tag="LK694_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK5052304.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2458..3042)
                     /locus_tag="LK694_001431"
     CDS             complement(2458..3042)
                     /locus_tag="LK694_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK5052305.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3243..4241
                     /locus_tag="LK694_001432"
     CDS             3243..4241
                     /locus_tag="LK694_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK5052306.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4353..4862
                     /locus_tag="LK694_001433"
     CDS             4353..4862
                     /locus_tag="LK694_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBK5052307.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4892..5551)
                     /locus_tag="LK694_001434"
     CDS             complement(4892..5551)
                     /locus_tag="LK694_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBK5052308.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5553..6317)
                     /locus_tag="LK694_001435"
     CDS             complement(5553..6317)
                     /locus_tag="LK694_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBK5052309.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6325..7002)
                     /locus_tag="LK694_001436"
     CDS             complement(6325..7002)
                     /locus_tag="LK694_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HBK5052310.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7039..8424)
                     /locus_tag="LK694_001437"
     CDS             complement(7039..8424)
                     /locus_tag="LK694_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBK5052311.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTDIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8634..8718)
                     /gene="ffs"
                     /locus_tag="LK694_001438"
     ncRNA           complement(8634..8718)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="LK694_001438"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8741..9256)
                     /gene="tadA"
                     /locus_tag="LK694_001439"
     CDS             complement(8741..9256)
                     /gene="tadA"
                     /locus_tag="LK694_001439"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBK5052312.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9363..10220
                     /locus_tag="LK694_001440"
     CDS             9363..10220
                     /locus_tag="LK694_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5052313.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10301..10636)
                     /locus_tag="LK694_001441"
     CDS             complement(10301..10636)
                     /locus_tag="LK694_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052314.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10703..12073)
                     /locus_tag="LK694_001442"
     CDS             complement(10703..12073)
                     /locus_tag="LK694_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBK5052315.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12151..12687)
                     /locus_tag="LK694_001443"
     CDS             complement(12151..12687)
                     /locus_tag="LK694_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK5052316.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12996..14477
                     /locus_tag="LK694_001444"
     CDS             12996..14477
                     /locus_tag="LK694_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5052317.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14580..15287)
                     /locus_tag="LK694_001445"
     CDS             complement(14580..15287)
                     /locus_tag="LK694_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBK5052318.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15366..15704)
                     /locus_tag="LK694_001446"
     CDS             complement(15366..15704)
                     /locus_tag="LK694_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBK5052319.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15815..16777)
                     /locus_tag="LK694_001447"
     CDS             complement(15815..16777)
                     /locus_tag="LK694_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBK5052320.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16792..18123)
                     /locus_tag="LK694_001448"
     CDS             complement(16792..18123)
                     /locus_tag="LK694_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK5052321.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18131..18811)
                     /locus_tag="LK694_001449"
     CDS             complement(18131..18811)
                     /locus_tag="LK694_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBK5052322.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19030..19509)
                     /locus_tag="LK694_001450"
     CDS             complement(19030..19509)
                     /locus_tag="LK694_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBK5052323.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19636..21315)
                     /locus_tag="LK694_001451"
     CDS             complement(19636..21315)
                     /locus_tag="LK694_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBK5052324.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21863..23365
                     /locus_tag="LK694_001452"
     CDS             21863..23365
                     /locus_tag="LK694_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5052325.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23378..24040
                     /locus_tag="LK694_001453"
     CDS             23378..24040
                     /locus_tag="LK694_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5052326.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24141..24344)
                     /locus_tag="LK694_001454"
     CDS             complement(24141..24344)
                     /locus_tag="LK694_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBK5052327.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24455..25639)
                     /gene="iadA"
                     /locus_tag="LK694_001455"
     CDS             complement(24455..25639)
                     /gene="iadA"
                     /locus_tag="LK694_001455"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBK5052328.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25796..27535)
                     /gene="yfcC"
                     /locus_tag="LK694_001456"
     CDS             complement(25796..27535)
                     /gene="yfcC"
                     /locus_tag="LK694_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBK5052329.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
BASE COUNT         8239 a         5770 c         4675 g         8969 t
ORIGIN      
        1 ttagtaacat gtcaataact attttttaaa ttttttagaa atgtgatata acatccactt
       61 cagaatcaac gctgttaaat ttatcatgta tttcttcctt ggtcaactat aaattaagca
      121 ttactttgtg aaatgatttt tcgatcaatt attattattc gttatacaac cgttttcaac
      181 atattttgaa aataaaatgc actaaaaaac tatctttagc agaacatttc attgtatttt
      241 ttggaccaaa caataaaaaa agaagtttga aaaaaattca aacttctttg ttcgcttaat
      301 ctgtatttta gaagaaaggc actccctcta cttcgtaagt tccaagaaca gagcgcattt
      361 gacctggttc ttcccaaata tatccttcgc ctaattcttc tctttgattc ttttgttcag
      421 tttcaaagac aaacaacgga acattttcct ctttaatatg tgtatttgtc aattccatca
      481 aactaatctt gctaacatcg atatgaacta tttcttttaa gaaattaagt atattcgcta
      541 aaccagtctt tccttcagaa agttccgcta acgcaaattc caacttgtca tcaattgaat
      601 ggaccaaaaa ttttttagta ccatcctcca aatgcaacat aattgtacca gcaactcttt
      661 tgcttgccaa aatttccacc tcgatcaact tgttctatgg cttacataaa aattctagca
      721 cattttcaga aaaaatacac tttttatttc ttattttctt tccgttctag ttcagataat
      781 gcttcttcta tccaaatagg taactgctcc gcaatagtgg taaatccgat cttacgattt
      841 ttattcgtga aatacctttt tctgcgatat ggaacagctg gatttgtttt ttctaacgtt
      901 cttaaagata aactgatttt tttggaatat tcatcaatgt cgatgatctg aactgttact
      961 ggctgtccga cttttaacaa tgaatggata tttttcgtat aaccggattg tacttctgaa
     1021 acatgaataa gtccttgcgt ctcgttatca agagaaacga atgcgccata aggctgtatc
     1081 cctgtcactt ttccttcaat tatttgtcca attttataag tcatctactt ccctctcttc
     1141 gcatttatca ttatactgta aatagctcaa aaaaagaagc gacgaaaatg actggaggtc
     1201 ttaagatgat cgaatttgat aaacagattt caagaaaaga tacagaaagt gtaaaatggg
     1261 acgcaattgc tgagacatat caaatggatg acttacttcc tttgtgggta gcagatatgg
     1321 acttcttgtc tcccggtggg gtagctaaag cgtttagtga atacatcaaa catggaatct
     1381 ttggttatgc cactctgtct gataaactat atcaggctat cattcattgg gaacgccaac
     1441 accatgctgt ggagttgacg aaagaaaata tcgtttttac aagtggtgtg ttaaccagtt
     1501 tagctgcagc agtacaaact tttacaaaac caggcgattc tattttgatt catgacccag
     1561 tttatcctcc attttcctct attatagaaa caaatcaacg tcacatcgtt cgaagctctt
     1621 tgctagaaaa aaataatcat ttcatcatgg acttgtctga catggagaaa aaaatcgaag
     1681 aaaacaatgt aaaagcaatg attttatgca atcctcacaa tcctggaggg cgtgtttgga
     1741 gcaaagaaga attgaaacag ttaagcgaat tgtgtttaaa acatcaagtg attctgttta
     1801 gtgacgagat ccatcaagat ttaactttat tcgatcatac atttacttcc atgctaacga
     1861 ttgatcctaa tttagatgat ctactagttg cttttacttc cgcaaccaag actttcaatc
     1921 tagctgcaat caaaaattca atggtattta tcaaaaatcc agagatgaaa gctgattttg
     1981 aaaaacattt agtgatgaac caacagcacg aaatcaacac ttttggactg attggaaccc
     2041 aagcggctta tgagacgggt ggagaatggt taagccaact gattccttat ctagaaaaaa
     2101 atgttcaaac agttaatcag ttttttgaac agtatcttcc gaaagtgaga ataatgaacc
     2161 ctgaaggaac ttacttaatg tggttagatt ttaccgccta tatttcagac gaccaagaat
     2221 tggagaacac tcttgttcgg aaaggaaaag tcgtattaaa tcctggcatc acgtttggtc
     2281 cttccggtca tgggcatatg cgcttgaata ttgcttgtcc agaagaaact ttactggaag
     2341 gacttaatcg aatcaagtat gctttagaga caatcgatta aatcatttat tcaaaataaa
     2401 aggacgagac cttactaggt ttcgtccttt ttcataatta ctccgtacca taccaaacta
     2461 ttctgaaatt tcaattgttt caatcactac atcatgtaca ggtcggtctt gtggaccacg
     2521 ttgtacattt gcgatttcat ctactgtgtc catcccttct agtacatgtc caaagactgt
     2581 atgtcggaaa tctagccaag gagttccgcc acctttatac gcttcaatga tttctgatgg
     2641 gaatccagca ccttctagtt gtccaagcat attagctggc acatttgtgt tatttacaat
     2701 aaagaattgg cttccgtttg tgtttggacc tgaatttgcc atagataaag ctccgcgaag
     2761 gttgaacagt tcacgactga attcatcttc aaaactttcg ccataaatac tttcgccacc
     2821 cattcctgtt ccagttggat cgccgccttg gatcatgaaa tcaggaataa cgcggtgaaa
     2881 aatcacgcca tcataataac cttttttgga aagttcaatg aaattttgaa ccgtcttcgg
     2941 tgctagttca gggaataatt gaacagtaat gtcaccacgg tttgttttga taacagcttt
     3001 tggtcctttt tcacttttta aatctagttg tgggaatgcc atattgtttc ctcctaatat
     3061 ttgcatactt cataataata ataacataat acctaacctc tcgtaaaaat actagatttg
     3121 ttttctttta tttttcatca ctagatacat tctataactt accgtttcga tttcgaattc
     3181 aaacattttt gagaagcatt ctcaattaaa ttctgctata gtgtgaaaag ggaggaattt
     3241 ttatggaaat ctatgacatt acaatcatcg gtgcaggtcc tgtagggatg tttgccgctt
     3301 tttacgcagg aatgcgccaa gctaaaacaa aaatcattga cagtctacct caacttggtg
     3361 gtcaattagc aactttgtat cccgaaaaat atatttacga tattcctggg tatccagcaa
     3421 taaaagcaag tgaattaatc gatcaattag aaaaacaatt aactactttt aaccatacat
     3481 tccatttaaa agaagaagtt ctctcgctta ctcgtgaaga cgaagttatc gagattacta
     3541 caaataaagg catccattat tccaaagcag tcattttagc tttaggaaat ggttcattcc
     3601 agccaagaaa attgaattta gataatgctg aatcatttga aaaccacgga ttggattatt
     3661 tcgtgaatga tttgatgaag tatgctggaa aaaaagtcgc tattgctgga ggtggtgatt
     3721 ctgctatcga ttggacgttg atgttagagc ctattgcaag cgaagtttac ttgattcatc
     3781 gtcgcccgga atttcgtgcg cacgaacata gtgtatcacg cttgaaatct tcatctgtca
     3841 acttgcttac tccctatctc atcgatggat tatcaggcaa taatggggaa ctgacagata
     3901 tccggttaaa aaaagtgaaa agtgacgaga cgattgattt gatgattgat tctctgatcg
     3961 tcaattatgg attctcttct aatttagaac atctttcttc ttggggactt gatagtacca
     4021 gaaatgccat tactgtaaaa tcagatatgt ctacttctat tccaggcgtt tatgcagctg
     4081 gtgacatctg tacctatgaa ggaaaagtga aactgattgc tactgggctc ggtgaagcac
     4141 ctactgcagt gaacaacgct ttacactata ttaatccaaa ggaacgaact cagcctggtc
     4201 acagcacaag cttatacgat aaaaatcttc gtcctagttg attttttata tctctttgca
     4261 tttcaagtta aacccgaaag cagtttcttg catttttttc atgttccgtt aacatgaaat
     4321 agagaaagaa aaaataaagg gggagcaata atatggtcgt caaaactgag actttagaag
     4381 aaaaggcaag agaactattg aaggaacgtg gcgtaactgt agaagatatc gcacaacttg
     4441 tcatgttttt acaaaaagac tatatttctg atttgacttt ggcggattgt actgaaagtg
     4501 tcaacagcgt gctaacaaag cgtgaagtac ataacgccat tatcactgga atccaattag
     4561 atatattagc agaaaagaaa aaattgatga gtccgttgca acatattatc gaagatgacg
     4621 aaggccttta cggtatcgat gagatcctag ccttgtcaat cgtgaatgtt tacggtacga
     4681 tcggctttac gaattatggt tatatcgaca aggtgaaacc tggtatttta aaagaactaa
     4741 acagccacga tggcgttcat gtccatacct tcttagatga tatcgtcgga gcgatcgccg
     4801 ctgctgctgc cagtcgatta gcacacaaag aaccagaaaa aagaagtcgc cttactcaat
     4861 aattcaacaa aaaaagaaag aaaacgtcta tttagacgag ttctttcttt tttgtttgtt
     4921 tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat aatacaaaaa
     4981 ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt ggatcagtaa
     5041 cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg ataaaaaagc
     5101 gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg ccgatttgaa
     5161 acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata aacaaaatac
     5221 tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt ttcacttcat
     5281 aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg atggttttga
     5341 agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc tcttgatcta
     5401 aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt ggtcgaaagt
     5461 tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac aatccagcat
     5521 attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc cagtgattgt
     5581 ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt aaaacctgat
     5641 aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt ttcatagtta
     5701 tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc tttatccata
     5761 atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt ctctacaaaa
     5821 gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt cttatctggg
     5881 ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac tactttttca
     5941 taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc ttcccatgta
     6001 aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata gacttttttc
     6061 ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta gactgtctct
     6121 ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc tggtgactta
     6181 gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc ttccacaaat
     6241 cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa atcaattaaa
     6301 tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa atgctgtttt
     6361 tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg tttttgctga
     6421 ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg gtcctttttt
     6481 ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag ttgtcttttc
     6541 ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc aatattcgta
     6601 aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat tatccgcttg
     6661 gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat aatcatatcg
     6721 ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt cacgataatc
     6781 ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta acagtgtcac
     6841 tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca aagcagctgt
     6901 cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag caatttctgt
     6961 tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac cacctatctt
     7021 ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat ctcctaccac
     7081 acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa tcgttggaaa
     7141 aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat tcagatcgat
     7201 tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc cattatagac
     7261 catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt tacgtaaaaa
     7321 gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac ccaatgtgac
     7381 atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat tgacccgccc
     7441 ttttggaata tcagttaaaa acagaccact gtttaatatt gctgcttccg tctttcctcg
     7501 ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga tatctagact
     7561 cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc cacgttctag
     7621 ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg tagggatggc
     7681 tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga cttcaccgac
     7741 atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt gaccttctct
     7801 gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt cttttgcaat
     7861 ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca cttgtgaacg
     7921 taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc agccattcgg
     7981 attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg tctggtgttt
     8041 cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat ttttatcgaa
     8101 taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca actcttcttt
     8161 ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa cttcattcat
     8221 cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac gatccacaaa
     8281 atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt ctcgtttacg
     8341 ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca gatcattcgt
     8401 atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt atcataccat
     8461 aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg ccttgaagca
     8521 aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga acattcattt
     8581 ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt tcattataat
     8641 cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac gcttcacaag
     8701 cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca ttcgttgacc
     8761 agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca gtcaaaatca
     8821 tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta aaccgctcgt
     8881 cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac gcgccgaaat
     8941 aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa ggctctaagg
     9001 taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag gcttccttga
     9061 ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg ttatatcctc
     9121 ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca tgaagcattt
     9181 ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct actgcatcaa
     9241 gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta ctttagcata
     9301 tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa gaggtgaaca
     9361 agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa aactctccag
     9421 catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg atcaaacaag
     9481 attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta atcaacgatc
     9541 aaaaaaaaca cccatggtat cactatcttt ttaaagatgc ttcttgtgtt caagaaaaaa
     9601 gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa aaagaatggc
     9661 aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat acagacacag
     9721 atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa atcaacggta
     9781 tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt ggttctttcc
     9841 attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt tttttaacag
     9901 agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg gctttacatt
     9961 actatacaaa agatactgta aaagatagta agttgatcaa aagttacata gaactgatca
    10021 aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc aatgtcagct
    10081 ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc gaaaaatttc
    10141 aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc tatcttttat
    10201 tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt ttgtcacaac
    10261 cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt ttttcaacaa
    10321 aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca ccataaacag
    10381 agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa tccaagtcac
    10441 tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat ttgtcataga
    10501 cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt acttttcccg
    10561 atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg attgttactt
    10621 ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata gcatagacaa
    10681 aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa aagttcttta
    10741 ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact agcaaaaccc
    10801 atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc cattcctttt
    10861 gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct aatgcatagc
    10921 taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt acgactttta
    10981 aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg aacactaaaa
    11041 gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt ttttcctgct
    11101 cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc atctgatttt
    11161 gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta tgaggccata
    11221 tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct gaactaacgg
    11281 attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc cagctaatga
    11341 tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt cctttatttt
    11401 ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag tatagcggag
    11461 attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct tctcctgaac
    11521 aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca agttctgcgg
    11581 gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt tctgataaaa
    11641 tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc ttcatttgag
    11701 cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact ttttcaccgg
    11761 gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta tattcatacc
    11821 cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca ttgctgtagg
    11881 gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct gccggtttta
    11941 gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa aaaaaatcgg
    12001 atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt aacgttgtcg
    12061 cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac gaaaaataaa
    12121 ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta agatattttc
    12181 caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt caagaaaatc
    12241 ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca attcaagcga
    12301 cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc gcttaaatag
    12361 cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat ggatagaaaa
    12421 aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt cagctaagta
    12481 gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc tgattgattt
    12541 gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact ctcttgcagt
    12601 aattgacttc attccatatt cttccacgta taccatactt ttgaacagca aatattcttt
    12661 actataattt gttactctcc ccatcattct tacctccgtc tcttttcatc tacccataaa
    12721 taaataacat aaataattct aaaaatatta ttgataatta tcaaaacata tttagttata
    12781 tccattaaaa atcaattttt ccgccttaaa aacaagaata tcttgacaaa ataccctagg
    12841 aaataaacaa tagaataaac aaggtaacaa aaaaattaaa taacgttata taaaaaagaa
    12901 ggcgacactc tgtgagaata acacagactg aaatgacttt ccactcacag tttttgtcta
    12961 actatataca aaaacagaat aaaaaggagg tatttatgct aattgaagca agaaaaaaag
    13021 agttacttgt tctacaaatg gtcattcaaa aaaataattg ttctttacaa gaattatcca
    13081 atgacttaca tattccaaaa cggacaatca aagaactgat ccgaaaaata aatgtatcac
    13141 tcaagcaatt tttattgatt gatgaattta tttattctaa tcataaagga gagatccaaa
    13201 ccaaagaaac cgatcaaact aaaaagttag ctgttttttc taagttaaaa ttatgctacc
    13261 taaaagaatc caatcgtttt aactatttga tgttactaat caattatcca caaactgctg
    13321 taccaaaaaa atatttactt gaacaacttt atatatcgcc ctcctatctc gaaaaactga
    13381 caaaacagct caatcattcc ttaaaaaaat tccatttacg gatcctatct gtaaaaaatt
    13441 gctgtactct tgaaggaaat gagttatcta tccgtctcta tctttacttt tttctttcag
    13501 atacatttgg aggtaacgat tggccatttt cctcacttga attaaatgca ctaaaaatga
    13561 acagtacaga tgagcaaata gaaaaaattt caaaacaaga aaaaaatatc tacttgctgt
    13621 taactttatt catattacga aacaaacaac aggcatattt tccaaaaact tcttctgaaa
    13681 tcactgaatt gatggactta ttgcagcata cacatgatta ttcagaatac tttacttctt
    13741 tttcagtgag tcgcccttgt gaagagacag aaacagagag agcctacttt aattattttt
    13801 tctattgttt ttttccaaat caaatctctt ttcgacaaaa acaggagatt ggttttttat
    13861 tttccacaag caaaaacctt atttgtcgac aagtcggtca attgatgact tacttaactc
    13921 aacagtttcc tctcaaccat ttagaaaata aaataccaat ttatttttat tttcttgtgt
    13981 tggcagttgt ttttttcgaa ctgttgggtg aatccagcag tagtttttct acactttgct
    14041 ttccagaagc cacgtccaat cattctagtt cttttataca ggaaaagaaa ttaagagaaa
    14101 aaatcgatac aattttttca aaaaatgggg aaagaaacca aatcagtcaa taccttagtc
    14161 aattgatcca acacttacta caaacagaaa cgaaagagca gttactcatc catttgcaga
    14221 taaccaaaca gtttacagga aattatcatc ttcaaagcca tttgcagcag ttatttcgca
    14281 aagatgctgt taaaattacc gcatattcag aacatgctga tttgatcatt acagatcagc
    14341 atgagaaaat ttcagatgaa acggagactt tctatctgga tacaacgcac aatacagtgt
    14401 tatttggcaa gttaataaaa gcggttcatg aactgtatat gaaaaaaggt tatgagaaca
    14461 aaacaaagag gatgtgacaa aattcttgtc acatcctctt attccaaata aatggtattc
    14521 aatcagctaa ctctcggtat tagctcagat caaagcgctg atttcacaac ttctttattt
    14581 taatctcttc ggttgccgcc gaacaataag atcaagcgca acagctgcag gaatgtcgaa
    14641 agcgctgctg caacataagt cagtgctgct gcaaataata cgtgtcttgc catcggtact
    14701 tcttcttccg tcagtagtcc gccttcactc aaaatttgga ttgctcgccg agacgcgtta
    14761 aattcgactg gtaatgtcac caattggaaa agtaatgcta aagaaaaagc gaaaatcccg
    14821 atattgatta acgtagagtt ccagctaagc aatacaccta ccataatcaa ggggaacgaa
    14881 atcatcgaac caaagttagc tgctggaaca agtgctgcac gaatcttcaa tgggacataa
    14941 cccgaagcat cttgtaccgc atgtccgcat tcatgagctg ctacacctat cgcagcaact
    15001 gaagtagact gtgcagttgc ttgagacaaa ctcaacatct tattacctga attgtagtta
    15061 tctgtgagat ctccggcaat ttgttgaaca ccgacattat ttatctgttg actttgtaat
    15121 atatattgtg ctgcttgtgt accagtcaca ttgtttttgc ttctcacttg atcgtatttt
    15181 cggaaagtac tattgacgta cgctgacgca attccggaaa taactaatcc aagaatgacc
    15241 aaaagaaatg tcggatctaa aaaaccatac attccataac catacatttt cgtttcactc
    15301 cttttcgttt actttatttt atcattagta gtaggaaaag aatgtgaaaa gaccttgaat
    15361 attctttaat tctttttcac aaattccgat ttcagtttca tcggtccaaa tccttctacc
    15421 ttgcaatcga tattatgatc gccttctact aaacggatgt tcttgacttt tgttccttgt
    15481 ttaatggcac ctgacgcacc ttttactttc aagtctttaa tcactgtcac gctatcgccc
    15541 tctaacagac ggtttccatt tgcatctaat accactaatc cgtcttctat gacagtttct
    15601 tgttcttctt tgttccattc gttcccgcat tctgggcaaa tcaacaagcc tcgatcttca
    15661 tatgtgtact ctgagccaca ttctgggcaa tttggtaatg tcatcttctt tctcctttaa
    15721 aatctttagt gagcactatt gtaacggaaa aagaagatct tgaaaagata catttgtgat
    15781 ttttactaca aaaggcacgt tttcgaacga agtatcattc tgtttttctg ctcttatacg
    15841 attggcatat atcttggatc tctttataga tttcctctac ttctgctaaa tcttttgcat
    15901 tcgcaccact tgctaatgga tggcctcctc catgatggcg tttagccaat tcattgatga
    15961 tcggcccctt agaacgtaaa cgtacgcggt aataccctga aggctgctga acaaaaatcc
    16021 cccaagccag cacttcgtca atcactccag gaagtgaaac gatcgaagct gtctctgaat
    16081 catcgattcc atatttatca agaatttctt gcggaaggat tattcttcct gcaccatttt
    16141 catccacctg gatattttga taaagataac cggaaagttt agcgactttc atcggcattt
    16201 gttcaatctc tctgttcaaa gcacttgcat caaatggata atttctcaat atcgaagcaa
    16261 tctctaatgt acgggacgtc gtggagggat acaaaaagcg ccctgtatct ccgacgattc
    16321 ctgcataaag taaccgtgca gccttaacgt tcatctttaa agattcagga aacatcaaag
    16381 caaattctgc aatcatctca ctacagctac ttgcttttgt atttacccac acaagatctc
    16441 cataagggtc atcattggga tgatggtcga ttttgatcag tttttctgcc aactgatatc
    16501 tttgatcact gattctgggt gaattagctg tatcagtcac gatgactagc gctccttggt
    16561 agaagacatc ttcaacttct tgcatttctg ctaaaaagcg aagcccttca atcgtttcac
    16621 ctacttgacg tatttctttt tctgggaaac tttcccgtaa tagttcggct aatcctactt
    16681 gtgatcctaa tgcatcagga tcagggcgca aatgcctgtg gataatgatt ttatcgtatt
    16741 tttttattgt atctactatt tcttgctgta ctgtcatttc actcctgctt tctaagaacg
    16801 ttccattact tgacaaacaa cgattgcttt ggaaacgatg gtgttttcaa taaacacttc
    16861 gatatccagt ttagcggaac gtctgccgat ttccaataca cgtggacgaa tgtctaattc
    16921 gctttccaat tggatcagcc gcaaataatg taggttcact tgttcaatca agatatttcg
    16981 tttttggttc atcaccattg ttttttgtgc gacatcagaa atgatttcgc taagtacgcc
    17041 aaaagaaatg gttccaacgc cattgaccat ttgtggtgct actacgaatt tgaacttagg
    17101 gttttccaaa cgatttcctt cacggtcagt atcaatcgta tggatatttc cagatatctg
    17161 atccgaaatc gtatctgcaa tctgcggttg tcgctggaca agctgcatcg ctttcattac
    17221 atcttgccgg gtaatcaagc cttccaatgt caagtcatcg gaaacgaccg gcatgacttc
    17281 tagcccatcc cagatcattt gatggctaac agaggcaata ctcattgttt tctttacact
    17341 gattggttct ttcgtcatca cccgatcgat gatctggttt ggcgatttcc ctaaaacatc
    17401 ttttgccgta acaatcccca tcaaacgatg atggcggttg acgactggaa aacgcgaatg
    17461 ctgcgtttct tctgacaaac gctgataatc agaaatcgta tctgtctgct gcaaataatg
    17521 agtcttttca gctggcatat agatatcact gacaagcatg atatcttttt tgatcaattg
    17581 gtcgcttaac gctcggttga tcatggttgc gactgtaaat gtatcataag aggtgcgtaa
    17641 gatcggcatt tcaagatcat cagctagctt tgcaatctca gcagtcgtat tgaatcctcc
    17701 tgtgatcaat actgcggcgc catcttccaa tgctaatttt tggacttccg cccggttgcc
    17761 gacgatcatt agtgatcctg gcgtaatata gcgttccatg gcatgtttag tcatcgcgcc
    17821 gatgacgaat ttattcaaga ccttgtctaa tcctgccgcc ccgcctaaaa cgtccccttc
    17881 aatgatcctg acgacctcgc caaatgtcaa acgttcaata tgttttttta atttgcgttc
    17941 gatacggatg gtccctactc gttgaattgt tgaaactagt ccgacatttt ctgcatcttt
    18001 gattgcacgg tacgctgttc cttcactgac attcaattct tttgcgatgc ttcttacaga
    18061 aattcgattg ccaacaggta aactttcaat atattctaaa atcaaatcat gttttgtcgc
    18121 cattttctgc ctacacttcc agcgtttccg agacgctcat tatttttcct tcactatctt
    18181 ccagtaactc tacaaaagat tcgggatctt gttcaatgac agggaaagta ttgtaatgga
    18241 ttggaactac tttcttggca cgaattgttt gcgcagcctt tgctgcatct tctggcccca
    18301 tcgtatagtt atcgccaatc ggcaagaaag cgacatcaat atcgaattct tctgcaagca
    18361 atcctagatc actgaaataa gcagtatccc ctgcatgata aatcgttttt ccttctgctt
    18421 gaataataat tccgctaggc tcgcccatat actgaacgat accgtctact tcatagcttg
    18481 aactatgctg tgcatgtacg aattttacca cgccgaatgg aaacgcatgt tttccaccga
    18541 tattcatacc gaatgaatct actcctctgc tgttcgcata atcagcaatc tcaacaatac
    18601 tgataattgt ggcttgattt tcttttgcaa taggaatcat atctcctatg tggtcactat
    18661 gaccatgagt aatcagcaac caatctgtct ttacttcttc tgcagttaag tctgtcaatg
    18721 gatttcctgt aataaacggg tcaatcatca cgcgttggcc attttccatt tcaattgtta
    18781 aacaggaatg tccatgatac gtaattttca tattttacac ctcgcgtttt ctctgtatct
    18841 tttttattaa aaacgatttc atcacttgca catcaatgaa aaaatatagc tgctaaatat
    18901 attttcattg gattgatagt acagtatgta caaagcaatt gtaacagata atacaggatg
    18961 caccaagtac caagcagata taatctcaag tttgaggtct ctgctctgct tagcttctct
    19021 tctcgtccct taactaatta aatcagcgta aataggatgg acgatctttt ctaaatcggc
    19081 tacttttatc cgaaggcttc tcccgatttc accgcttgac acaattactt cttctttttc
    19141 ttttaaagaa ctgtctgcta aaaaacaata atcaggatgt gcgagtttta ttccaatagg
    19201 ggtgtttgct ccgtggggat atcctgtatg cattaataca aaatccattc caggaaaacc
    19261 aactttacga tctttcgtca cttgtctcgt ttttttgtaa tctaagtgat cgttcaaagg
    19321 aatcaatgcg atgatcaccc cagttttgtt tcctttcact acgagagttt tacatatcaa
    19381 agctggatca atccctcttt tttgtagttc ttctgtgaaa gattcttcta ccacatgact
    19441 aaaatcaagt ggttgatagc tgatcttttg ttcttctaaa taacgttcga cttttgtttg
    19501 actcatcatt gattcactcc ctttattcta tcgtttatta taaagcgagg gatgctgtat
    19561 tcaaaaatta tttgtttttc gcaaaaaaag agcaagacga gcactctata gagaggttca
    19621 tcttactctt tttgtttatt ttttatttcg acgttgagta gcttccataa tgactggcaa
    19681 gatcactggg cggcgttttg tttgttcaaa taagtaacgg cctaattgat ctcggatgtc
    19741 ttgctttaat ttgctccact caaaatcatt gctttctaaa tgtttctcaa cgattgtttc
    19801 aatcatattg ctgctttctt tcatcagatc acggctagct tttacgtaaa cgaatccccg
    19861 tgaagtaatt tgaggttttg aaatgatttt cttttctcgc cgactgatag tgacaaccgc
    19921 tacgaaaatc ccgtcttctg acaagatttt tcgatcacgt aaaacaatgt ttccgatatc
    19981 acctatgcct aatccatcaa tcattacatt ttctgcactt gtagttcctg caacactcat
    20041 acgcttgttc ttatattcca gcacgtctcc gcgtccagta atatagatat ttttgtatgg
    20101 catccctact gcatgagcca aatctgcatg agcagctaat tcacgatact ctccttgaac
    20161 tggcacaaaa tatgttggtt tcatcaaatt gatcattaat tgcaagtcat taggattggc
    20221 atggccagaa acgcgcatat tctcagagat caacttcact gttccaccag ctcgataaat
    20281 gatatcttcc gttttcgcca cgatggtttc catcgcgatg cttggcgtag tagtgatata
    20341 aaccaaatct ccttcttcaa tcttgacatt acggtgtgta ccgtttgcca ttttttgcag
    20401 agacttgatg ggttcaccca ttcggccagt ttctaaaatg atcagttctt ctggatcata
    20461 gtttttcatg tctttcaacg taacaagcag atcttcactt ggtaattgaa gtttttctaa
    20521 acgcatcgct gtgcgaataa ttcgttcgaa atcttgacca gtcaagacaa cttttcgttg
    20581 tgctttttct gctgcatcca atacttgttg cactcgttgc aaattacttg ctacacaagc
    20641 gacgatgata cgcccttccc aatatttgat cgtatcaaat acttcatctg ctatttgggc
    20701 ttcagaagct acaggctgag gattttctgc atttgccgat tcacttagta aggctaagac
    20761 tccttctttg ccgatttccg ccaatcgtgc ataatctgtt tgatacattg ggatagccgt
    20821 ttggtcaaat ttaaaatctc ccgtataaac gatactacct tcttttgttt tcagattgat
    20881 ccccactgaa tctggtatcg tatgagtggt acggaaaaag ctcactactg tttcaccaaa
    20941 gtctatttct gtatgctcat ctaccacatg gaaatcctta aatcctttgg ctgcttcatt
    21001 acgactgaca ttcaattttg ctaactcgat cgtcaactct gtcccgaata ctggtacaga
    21061 aatttttgac aacaagtaag gaagtgcccc gatcgcatct gcatgtccat gtgttaaaaa
    21121 gacaccagct acacgatcaa tgttttcttc taaatacgta aaatcaggga ttacgacgtc
    21181 gatccccaac aactcatttt caggatattt tagcccacaa tctaaaataa aaatatcctc
    21241 gcccacttct gcgacataca tgttttttcc attttcacga acgccgccta atgggataat
    21301 tttaatggta ctcacaaact tcacctcttc ttatgacatc caagtttaaa gatgtcgatt
    21361 ttcttcttta tctaaatata tgagccattc caatgaagca tatgcaaaat cactatttct
    21421 caacttatgg agaatcagca ggaaaattac cagcacatcc gcgttctgac agttggatgg
    21481 gcattttatt ttggatatac ttcattaaca aaatggatct tttgcgaaac gctatagcca
    21541 gaatacttat ataagtattc agctgttccc cattattata caacaaataa actaaatcgc
    21601 tagtatcagc aattgttaga acaactatgc acgtaaaaat ccctctgtag catctaggat
    21661 ttcttttgct attcctcttt catttcttct tcgtcggttt gaaacatact tttttcattt
    21721 taccacattt tttgcaataa ctatagtgac tttttatata tacagaacct catgttgatg
    21781 gtgtgcaaac agcataagat ttaagctagc tttaagatag gttgtctatt atcaaatgca
    21841 tctactagga gggatgcaaa atatgaattt tatgaaacgt gcatggaaaa gtacaaaagt
    21901 aaaatgggga cgttcattgt tattgtttgc cgtgttcaca gccattctta tttttgtgct
    21961 agctggcctc acgattcgaa gcgctgcaga agaagcagcg aatcaagcac aaaaagaagt
    22021 tggagcaaca gtgactttaa gtgccaatcg agaagcttca tttaaaaaac aggaacaaac
    22081 ttcttcaagc ggttctacca ctgaagaacg tccagatcca ggcagttttt ctcttacacc
    22141 agtttcctta acggacgcgg aaaaaatagc tgaattagat aatgtaaaga gttattcctt
    22201 tgaaagttcc gcctcagcta tggcaaaaga tggaataact gccatcacta gttctgattc
    22261 aagtgaagac agttcagaaa ctactgatgc aggcggtgaa cctgctggag gaatgggcgg
    22321 gaatccgcaa atgactcaag cagactttca aatcagtggt gtgtcagcaa ctgcacaaaa
    22381 tagtaatttt tctgatggaa cagcaaaaat cacggatggt gaaggaatag atgacagcga
    22441 caaaggcaca aacaatgtag tcattgattc tactctagca gaaacaaatg acctggcagt
    22501 cggagatact ttcacaatca cgagtactga agatgaagat accacttatg aaatgacgat
    22561 caaaggaatt tatgaatcca gcgaaacaag cggcagtatg ggtatgaaat tcaacttcat
    22621 gaatccgtcg aatacacttt acacttctta cacttttgca aatgaactaa atgggacatc
    22681 tgacgataac acgatagatt ctgctattta cactctatct gatccagata aaatggatga
    22741 gtttctcaaa gaagctgaga agctgatcga cacagataca tttagcttac aatcaaatga
    22801 ttccatgtat cagtcaatgt tggaaccttt aaacaacgtg gcaagttttt ctaaaaatgt
    22861 tgttttacta gtggctgtag ccggtattat catacttact ctgatcatta tgattaccat
    22921 tcgggagcgt cggcatgagt taggcgtctt gctatcttta ggagaatcca gaagtaaaat
    22981 cattctccaa ttgtttaccg aagtggctat ttgtatgatt cttgcattag gagttgcttc
    23041 gcttagcgga aacgtcgtcg cgaatgccgt gggacagcag ctacttgacc aacagacaga
    23101 aaccacgaca caaaaccagc aagctcctgg cggtggacaa atgccaggta acagacaaga
    23161 acaaggcggt caacgcgatg gtcaacaagg aaccacgaat aatccatttg ctgtttctga
    23221 tcaagtcagc gaattggaaa tcaatgtcaa acctgcacaa ttgggactat tagccggagt
    23281 agcatttgga atcagtctgt tttctgtctt tttagcttct atagggatat taagattaaa
    23341 cccaagaaaa atcctattaa actaaaggag gaatttgatg ttacggacag aacaattggg
    23401 attttggtat caagatccgc aagatgcgct ttataaagat gtagaactga atttcgaagc
    23461 tggaaaaatg tacgctattt taggagcaag tggttctgga aaaacgactt ttctttcgct
    23521 tatttctgga ctagatgcac caaaagaagg aaaggtatac tatcaagatc gttcactgaa
    23581 caaaatcggg ttaagaagtt atcgtaaaaa tgatgtctcg attattttcc aagcttacaa
    23641 cttgcttcct tatttatcgg ctctagacaa cgtcatagca gcaatggaaa tcactaagac
    23701 caagcaagag aattatcgac aagttgcttt agatcgactg gctcgtgtag gaattgatga
    23761 aatattagca aaaaaacagg tgaccaaact ttcgggaggt cagcagcagc gagtcgctat
    23821 cgttcgtgct atctgctgtg aacatcaact aatcgtagcg gatgagccta ctggaaatct
    23881 agatgaaaca acttcacaag atatcgtcaa acttttccaa gaaatcgccc atgaacaaaa
    23941 tcgctgtatt atcatcgtga ctcatgagat tgaagtagca aaaagctgtg atgaagtctt
    24001 tgaactaaaa aataaaaaat ttcaaaaaat cgaagtttaa aaaacaggat aatgatcaaa
    24061 taaaaaatcc gaaccatatc tgtctcgcag atatggttcg gattttttat ttgcttgatt
    24121 caattagtaa taagtggttt ttattttttt tcgtcttttc gagaaaactt gtctttgact
    24181 tcgtcgccta aatttcgagc tttttctttg atgtcagcac ctagtgattg tgctttccct
    24241 tctgtttctt tgctcttgtc gtctgttgct ttgccgtatg cttcttttcc ttcaccgact
    24301 actttatctt tcatatcttt agaacgtcct tttaaatctg ccattgtgct tcctcctctt
    24361 agtttgttta ttgatctacc ttcagggtaa catcaacaac agatacagac aaacagaacg
    24421 ctcgttttct aaaaaagata gttagagctg ttgattaaaa acttttttcg aaagtccccc
    24481 atactttgac ttctttttct tggaccatta tttctccttt agcgataact gtatcaatat
    24541 caagcgtttc tttatccaag atacacagat ctgcatcagc accaatttcg atttttcctt
    24601 ttttatccaa tttcaatata cgtgctggat tactggttat agctcgtaag gcgatttcca
    24661 aagggatact ttctttttgc acagcttctt tgatccctac tagcaatgcc ttggcacttc
    24721 ctactcctaa ccctaggaaa tgattgtttt catcaaaata aggcaagctt ccttgtccat
    24781 ctgaggtcat cgtgaaatta tctaaagaaa catcttcctc gatcaatcgt ttcagtgctt
    24841 tgctgaagcg aacttcacca tctgtcttct cccaaaaatc aggatcttcg ctagcagtga
    24901 agtcaatcgt gccaccacgt ttggcaaatg ccacgcaagc ttcaaataat tctggtgtgc
    24961 gatttgcatg agtaggcaaa aattgtgtga tcggaatttc tgtttcttct acgattcgtg
    25021 tcaatagctc tagctttctt tttcctccgc ctaagtggac attgatgatc ccagcttttc
    25081 cggataacat gcctcctaca cgagcatctg ctactgcacg agcgaactct tcaaaacttg
    25141 gctgagaaga acggtgatcg gatactgcga tttctccgat tccgataatt ttatcaattg
    25201 tcaaaaagtc tttgatgata gaatctgtta ctgtttttac cggcaatcgg tacgaacctt
    25261 cgtatacata agtggagatt ccttctgctt ccagaccttt cgctttgctg atcaacgcag
    25321 tcatatctct accttctccg tctgttccca aacaaccgac aactgtcgtt acaccagctg
    25381 tagtcaattg gctcaatgtg acttctggcg ttcgattttg ataaccgcct tctcctcctc
    25441 cacctaaaat atggaaatga cagtcaatga aacccggggt tagtactttt ccctcacctg
    25501 aaagttcttc tacttccact ccttcgtagc cgccttctag ctgttcttct acagccaaaa
    25561 tcttcccacc ggcaatcagt acatccattt ttcctaagta atctggtgca aacacttcaa
    25621 tttgtcgaat gattttcata catttttcct ctttctttaa gtataactga tgataggatc
    25681 ttaaaaaata atccaaacga taggatcttt actcctttga ttttaccgta aaatgacggt
    25741 tcttggagtc cttatctact gtttggatta cttttttatt tacaggtctc cagtactact
    25801 gcagacctat tgcaatggca atcatgatga aaatagttcc taaaacaaac aggaatcctt
    25861 gcatcttgat ttggaatctt gcccattttg cccattcgat ccgtgcaaca cctagcacac
    25921 caatcaagct ggctgatacg ggtgtaaaag cgtccataaa acctgaacca agctgataag
    25981 caagaactgc tacttgtctg gatacaccta ctaaatctgc cagtggagcc atgattggca
    26041 tagtcaaagc tgcttgtcct gaatttgatg taacaaccaa gttgaacaaa ctttggaaga
    26101 tgtacatcgc ccaagcacca atgacagctg gaacaccggt caacgccgtc ccgatcccat
    26161 gcaaaatcgt attcaaagct gaaggaacag atgcatcaga tcctcccaag acaagaagaa
    26221 tccctttggc catacctact acgattgctg taccagctaa atcagctgca ccagattgga
    26281 aagaagaagc gatgtcattg actttcattc catctaattt gaaaagaaca gcaatgattc
    26341 cagcaactaa tcccatcaca aagaattgag aagcgatttc tggaatgtaa tagccttttt
    26401 gagtcacgcc ccaaacgatc cagacaagga caactaacat ctctaataag atcaatttat
    26461 gcccaagcat gaatttttta ttttcatcag atgtcttttt gatatgagaa cggaaataat
    26521 catctgattt gtaagtcaac gaactttctg gttttttacg gattttttct gcatatatca
    26581 tcatataacc agcggctaat gcagtaacaa ctacccacat aatcaatcgg aatgtcgcac
    26641 cagacaacac ggggattcca gcaatccctt gagccaccgc tacgctaaac gggctcatcc
    26701 aagaggtcgc atttccgact tgagatgcga cataagtcac ggttactgct acgatcgagt
    26761 catatcctaa cgcaatgaca aatggaacca tcaccattga gaatggaatt acctcttctg
    26821 ccatcccgaa tgtagcgcca ccgaatgaaa aagcaaagaa taatagcggt aaagctaaac
    26881 gctccagtcc cttagtttta ctgataaacg catagatacc tgcgtcgatc gctccggtac
    26941 gcataataat accaaaagca cctccaacaa caaggatcaa tgcagcgatc cctacagcag
    27001 atccatactt atctccagat actagtcctt caaaaacaaa atttaagaaa ccaaagccgc
    27061 caaaatcatc tgttccccat attttggctg ttttatgcaa ttttttagaa gtatcataga
    27121 tattttcgcc gtactgatcg taaagcacat catctgtcaa ggagatttca tctagttttt
    27181 cctgcgtaag atttttttct ccgtcagcaa tgactttttc caatcctttt tccggaacat
    27241 ccagtttttc acgttctgct ggatgatctt gcagttcttc taattgatca aacacatacg
    27301 acttatccag ctcgtaagca taacggaagg aatcttgacg taaaacagta cgagtacttg
    27361 tttcgccatt tgcatctttg tattcaatat cttgcgtgct gaactttcct gctggaacga
    27421 caaaagtcag tatccatgca aagatgacta caccaaaaat aatgacatac gtatggggtg
    27481 tcgataattt agtgaagtcg cgtttcggtt ttcctgaaga atttgttttc ttcaaattta
    27541 tttcttcctt tcttttttca gtaattttga tgaataaaaa atggataatc aaatgaatta
    27601 tacagtaaaa atgacatttc cacatgaata atttattaga aaatcacttt ttt
//
LOCUS       DAFBQH010000031        25239 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.031, whole genome shotgun sequence.
ACCESSION   DAFBQH010000031 DAFBQH010000000
VERSION     DAFBQH010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.031"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(43..2187)
                     /locus_tag="LK694_001457"
     CDS             complement(43..2187)
                     /locus_tag="LK694_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBK5052330.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVCNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2544..3917)
                     /locus_tag="LK694_001458"
     CDS             complement(2544..3917)
                     /locus_tag="LK694_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK5052331.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3917..4312)
                     /gene="rbsD"
                     /locus_tag="LK694_001459"
     CDS             complement(3917..4312)
                     /gene="rbsD"
                     /locus_tag="LK694_001459"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBK5052332.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4340..5257)
                     /gene="rbsK"
                     /locus_tag="LK694_001460"
     CDS             complement(4340..5257)
                     /gene="rbsK"
                     /locus_tag="LK694_001460"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBK5052333.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5365..6378)
                     /locus_tag="LK694_001461"
     CDS             complement(5365..6378)
                     /locus_tag="LK694_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBK5052334.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6459..7058)
                     /locus_tag="LK694_001462"
     CDS             complement(6459..7058)
                     /locus_tag="LK694_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK5052335.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7244..7945
                     /locus_tag="LK694_001463"
     CDS             7244..7945
                     /locus_tag="LK694_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5052336.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7955..10666
                     /locus_tag="LK694_001464"
     CDS             7955..10666
                     /locus_tag="LK694_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5052337.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11117..12745
                     /gene="treC"
                     /locus_tag="LK694_001465"
     CDS             11117..12745
                     /gene="treC"
                     /locus_tag="LK694_001465"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBK5052338.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12805..13986)
                     /locus_tag="LK694_001466"
     CDS             complement(12805..13986)
                     /locus_tag="LK694_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK5052339.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14238..15761
                     /locus_tag="LK694_001467"
     CDS             14238..15761
                     /locus_tag="LK694_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBK5052340.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15830..16558)
                     /locus_tag="LK694_001468"
     CDS             complement(15830..16558)
                     /locus_tag="LK694_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5052341.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16571..17852)
                     /locus_tag="LK694_001469"
                     /pseudo
     CDS             complement(16571..17852)
                     /locus_tag="LK694_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
     gene            complement(17993..18595)
                     /locus_tag="LK694_001470"
     CDS             complement(17993..18595)
                     /locus_tag="LK694_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBK5052342.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18840..19046
                     /locus_tag="LK694_001471"
     CDS             18840..19046
                     /locus_tag="LK694_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052343.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19358..20077
                     /locus_tag="LK694_001472"
     CDS             19358..20077
                     /locus_tag="LK694_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBK5052344.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20158..20841)
                     /locus_tag="LK694_001473"
     CDS             complement(20158..20841)
                     /locus_tag="LK694_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBK5052345.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            21059..23245
                     /locus_tag="LK694_001474"
     CDS             21059..23245
                     /locus_tag="LK694_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBK5052346.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            23591..24001
                     /gene="spx"
                     /locus_tag="LK694_001475"
     CDS             23591..24001
                     /gene="spx"
                     /locus_tag="LK694_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBK5052347.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            24015..24371
                     /locus_tag="LK694_001476"
     CDS             24015..24371
                     /locus_tag="LK694_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBK5052348.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            25044..>25239
                     /locus_tag="LK694_001477"
     rRNA            25044..>25239
                     /locus_tag="LK694_001477"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         8452 a         4493 c         4933 g         7361 t
ORIGIN      
        1 aaaagggagt gtgacagtgt cacatcccct ttttaattta ttctatttct ttaaaggcag
       61 atcaagccag aatgatttgt ctaaacttaa caaggttagt acaggaacag ttgcagcttt
      121 aactgtacga gtagttgttt ctggtatctg tcttgccgta aaattgttcc acaaatccca
      181 gattgaagcg acacggaaga tggaagaagc gttgttgacg cgttttatcg ttgaaaattc
      241 taatcctgtg attggcacgc catctgttgt ccatttgatt ggcacatcga atcgcttaga
      301 catattgagg atttcagcgt ttgcttcttc agttgttgct gcttcttttt tcggttcatc
      361 atattttgtc aggtcgtacg cttcgatgat cgcgttcaat ttttgtgaat attggcgatc
      421 tgtagcataa cgtccttcca aatatttggt tgcttctttg tagtcttttg tttccgactt
      481 ccaagttggt ttgtagaaat caggattgcc agtcagtcca tttttgatca agtcggcata
      541 gtcttccaaa ctttccttgt aagaaggata tttacggaat tgtgcgcgga tttggtagct
      601 ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg ctgcttcctt tatatgatcc
      661 tttgatacca aaaaggttat gatttggttc gcttgataat aagctgtttc cagatccact
      721 ttcaaggata gcttgagcca tcatgactga tgcgtagatt ccttcttttt gcccgatttc
      781 ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt gtttggtttt gactgaaatg
      841 gaacgagcca tctaatccat ctaaaataga tgagccgcct gtcagcgaac ctgaagcagc
      901 aggtaaacct gctaagttta ctcggacagc aaaatcaggc gtgaaatctg aaatagaagt
      961 tactcgaact ttatctcctg gttgaggcgc atggataaat tgtccgttcc caatatagat
     1021 acctacatgg tgagtaacac cggaagaagg tccccagaag acgaggtcgc caggttgggc
     1081 ctgacttact ggaatctgcg tacctgcata ttgctgttcg cctgtccagc caccgatata
     1141 atgaccggtt gctttcatat atacgtaata agtcaaacca gaacagtcaa aagtatttgg
     1201 accttttgct ccccatacat atggttttcc aagatgcttg aatgcttctc gaacgattgc
     1261 ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca attggtgatt ttacaacaaa
     1321 atcagaagaa gcagaagatg gattctctat accgctttga tttgctgtat ttggacgaga
     1381 tgaagcattg ttcgttccgc cattcgatga accattgttt ggatggtttg gttctctctg
     1441 agtatttcca ttgttattcg atccagatgg gtttgtttga ttcgaagaat tcccgctgtt
     1501 cgaattattg ttattattgt ttgaattatt tgaaccatta ttattgttgt tattacacac
     1561 atctggtttg tttggcgttt ccggatctgg tgtactaggg acttcttcac taggatcagt
     1621 tggtttctct gttgaagtat ccggtccagc tttaaattct gcagtaatag ataaattgtt
     1681 cgtacctact gtaaaggtct ttgcatcata atttacttct ttggcaccat taacagcgac
     1741 tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa gttaccttcg tttccgctaa
     1801 taaaccttct accgttccac tttccgtaat atccaaaggt tgctgttcag gggaatccgc
     1861 tgtcgaagca gtcagttgga tcttaccaat caaagaagga gcaattgtaa gggaatatgt
     1921 ctcggctggc ttttcatttt ctgatggatc agccggatca atttcagttg gctcggtcga
     1981 tcctgttgtt gagttagttg ttccagttgg gtcttctgta aaagaatccg ttgtttcact
     2041 agtagctgag ctggatgtag tcgtatctgt tgtgactgtt tgatcagcta tttgattatc
     2101 tacggcaaat acggtattgg tcgacaaaac agacggagca atcagccaag tagcagctag
     2161 caaacgtatc gtttttttat ttttcataat aaactagtcc ttttcctttc attgattttc
     2221 tgtcttattc agcattcatt agacattttt tggaacagat aatcgtgtcg aaaagctttt
     2281 tcttttcata aatctgtctg tgtattctaa ataaaacaat ttgtaaataa gcaacgagca
     2341 ttcattcgtt tttctttgac gttcatttta ttgcatagac cacctttgtt tggggataaa
     2401 caagtgggga taaaaatggt tatgcatatg taaattatac caaagtttgg aaagtatcac
     2461 aaaaatttat aaaaataatg atcctaaaaa gaaaaagact gaaatatgtg ataaacatct
     2521 catacttcag tctttttcat taactattca taagacatta ggtcttcatc aaacccgcta
     2581 acgagagtag tgatcattgc tacgactaga ctaacagcca gtcctgctaa gtaccagaaa
     2641 aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac caaatacaaa agcgatcgct
     2701 ttcacttggg caaatcccat tacagctcca ccagcagctg caccaatgca agcagcaaca
     2761 aatggttttt tcaatgggat cgcaattccg taaacagttg gttcggtcac tcccgcaagt
     2821 aaaatcgaag caaaagcaga accggagatc gaacgggttt ttgaacgttt acttcgcaga
     2881 aatacaccta aagctgcacc agccattcca aaattggctg ctcctgtcaa cggaacgatt
     2941 cggtcgaacc cataagcaga gatattactg atcatgacag ggtttacagc ccattggata
     3001 ccaaatatta ccaaaatatt ccaaccacca gcgatgattg cgccactgat cggtccgtta
     3061 atatccatca tccaagtaat tgcggcagct aaaccttcgc caaggtaaac acccgcaggc
     3121 ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg aaagtaaagg tacaaatact
     3181 aattgcatat tttcgcgaat atattttttg aggaattttt caacaaaaga ataaaagaaa
     3241 atacctaaaa tcgccggcat gacagtagac gtatactgca tcagtactac aggaatcccc
     3301 agaaaatcag tagctgcttt gccactgtct gaaagtaaag aggtcagatt cggttcaatc
     3361 agtgctgcag caatgacagc cgcaatatat ccattcactt taaacgtttt ggcagcagaa
     3421 aaagcaagaa agattggcag aaaataaaag acggcattcg acgcagcaga caaaatatga
     3481 taagttccgc ttgtttcact aagccagcca atcgtagtcg ctaaaacaac cagacctttt
     3541 aatataccag aaccggcaaa cagcggaatg atcggcgtaa agatttccga tatcaaacca
     3601 aatagttttt cgatcggatt tagcttttct ttcggagctg aatgatcgat ttgacgcgtc
     3661 aattccttgt aagcaggagc caatccctga tccatgatta tttctgcata tctatcgagt
     3721 aaaatgaaat acttaatgga agggatttgc tgttttaatt tttctgcatt ggctactggc
     3781 tgttttttca atttgatacg aagccgagtc gcacatggaa ggacagaatc aatattgtct
     3841 tttcctccca actgttcaat cagcatatga atctgctcgg acagtaccat ctgttcagga
     3901 ttcattgtcg tttccattag aagaccactc cactcactaa tataatattg gcgtaaggtg
     3961 tcatttcgcc tgtacgaata aatgcatgac agttattcaa ctcttttttc atttcttcat
     4021 gtgggataaa gctaatcggt gtatctggta acaataattt gattttttcc aatatttccg
     4081 ggttcattgt ctttatttct tccgccaatt caatcgattg tacctcaagt tcttctaaaa
     4141 cgttttctaa aacagataaa aaacttggaa gaccgtttga gacagccaaa tctatttttt
     4201 cagtaccaaa ggggacaggc atgcctgcat ccccgatact taatttatca aagtgcccca
     4261 tttgtgcgat tactcgtgaa atatctgaat tgattacttt tccttttttc atgtttgttt
     4321 cttcctctca ttgttaattt tagactgctg attgttctaa agcttcgcgg taagggatag
     4381 aaggttgcgc accataagac tgcacagcaa cagaagaggc tagatttcca taaaggatcg
     4441 cttctttcaa gttactaagg tctggctgaa gtgtgctgct taatgcgcca ataaatgtat
     4501 cacctgcggc tgttgtgtca acggcatcca ctttaaaagc agggacgata ccgtgttctt
     4561 tttctgtatg atagaaagcg ccggcactgc ctagtgtaat gattactgtt ccaataccta
     4621 attcatggag tttttcggct gctgcgacca aactgttatg atctgtcact ctgattcctg
     4681 taatgatttc tgtttctgtt tcatttggta taatgatatc tgtcttttca agtagttcag
     4741 taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt tgtttttcct gcgtcttttg
     4801 caatagaaaa agctaggatc gtcgcatcta agctactttc aaactgagca ataataaagt
     4861 cactgtttgt aatcagttgc ttgtttttta ggacttcttg ttcatggaaa gcattatttg
     4921 cccctgcatg gatcaagata ctattttctc ctgctgcgtc taccataatc attgcttgtc
     4981 cggttgtggc gccttggatt tcagctatcc cagatgtatc gatgttttct tttgtcagta
     5041 gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc tccaataaaa gatgtccgtg
     5101 cgcctgaacg tttggctgcc actgcttggt tggcaccttt acctccgcca gcgtgaaaaa
     5161 tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt ggttaaacga agggtagtgt
     5221 ccatattaat acttccaatt actgtaaccg tgttcatttt acaaacctcc aactatctca
     5281 tttattttga tgggaaaatt attttttaaa acgttttagt aaaacgtttt aatagttcga
     5341 taaaaatata gcacaccttg aaagctattt caagggtgca gttgaaaatc ttttttctaa
     5401 tcgtacaggt aatctttttt cttcccattc tttttctggg aattggatcc gatctaacaa
     5461 taacttggca gatgtttgtc ccaattcgaa gattggttgt gcgatcgttg tcagtttcgg
     5521 cttgatatac tcgcacatat ctatattatc atagccaata atgctgtagt cttcagggat
     5581 gctttttcca gcttcctcta atccgcgata taatccaaaa gctaattcat catttaaagc
     5641 aaacacacca gttgcagcag aatcaagaag ttctgctgtg atctggtagc caccttgttt
     5701 tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg atttggtaaa catcaagagc
     5761 agatttaaac ccctcaatcc gagcgtggac attttccggt ggattttcag gatagacaag
     5821 ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc gctaggtaac caccgcgaaa
     5881 atcgtctgtt cgtacggcat cactaaatcc ttcagatttt ttctgatcca aaacaatgaa
     5941 gggccggcct tgttttttca aattttcgtt gattgcatct gtagagacag cagaagtcgc
     6001 gataatgaat ccatccacac ctcgacgtgt gagctcggct agatattcga tttctttttg
     6061 atgatcagaa tcagcattac ataatatcgt gacgaaattc tgcttataca agatatcctc
     6121 aattcctcgc atcagtgtac tgaagaatgg attcgtgata tcaggaacga gtacaccaat
     6181 tgttttcgtt tccttggtaa tcatttgtct agcaaaatag tcaggctgat agcctaattc
     6241 ttctttggca gctaaaactt tctgaacagt tttcggactg aatttctccg tatttccatt
     6301 taatatcaag gagacagttg tgattgaaac gccggaaagt ttagcgactt ctttgattgt
     6361 gatctttttt ttcaccatgt tcatactcct atctaaatcc tgtggttatt ttcccatgaa
     6421 atcaaaaaga agtaaatgaa aatcacatgt gccagtactt atttgctttg ttggatagct
     6481 tcgattattt gtttcgttga gcgtactcgt gcagttttcg gaaatatctt ttttatgaac
     6541 aattgatggg attctttgtc acgatcactg caagcatctt cagcaagcac gatatggtac
     6601 ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt ttgttgtcgc tacaccgctt
     6661 aaaatgatag tttcgattcc acgccggcga agttgaagat caagagaagt accaaaaaaa
     6721 gcacttggat tgtattttgt gatagtcaac atattatctg tatctttcaa agaatccttc
     6781 aaaagtaaat cagtgaattc tggaggtaca gaagcagtca ttccacccat atcagtttgt
     6841 tgactcaaat aagggaaagt ggagatgtct acgttcacta aaactttcaa ggcagcggta
     6901 cgtttcaatg ttcttactaa caaatcattg tttgtcagaa tagatgctgc agtgtgtgga
     6961 gagagtgtac cgttatttac gatctctttt tgcatgtcga tcgcgacaaa agcagttttt
     7021 gataaatcaa aaatcatatt ttcctttaaa tcattcatga tgttccttct ttcaaataac
     7081 aatgtttttt atgtaagtaa atcataaatc gtttatttgt caactaaatt aaaggattcc
     7141 tttctttagt ctagagaaat tcagtggtaa aataaataag tgtgagaaaa cgcattcgat
     7201 gcatagatat agcgagaaat atcgtgaaaa tggaggaatc agagtgaagt atgtcgaagt
     7261 aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc attacagcaa atgacgatat
     7321 ttctttttca attgaaaaag gcgaattagt gattatactt ggtccaagtg gagcaggaaa
     7381 gtcaacgatt cttaatattt taggtgggat ggatacacct gatgaaggtc agatcattat
     7441 tgatgacaca gatattgcgc aattttcaga taaacagctt actgcttacc gacgaacaga
     7501 tgtagggttc gttttccaat tctacaatct cgttcctaat ctaacagcaa aagaaaatgt
     7561 ggaattggca acggaagttt ctcctgatgc gcttgatcct gtagaggtat tgaggcaagt
     7621 gggcctagct catcgattga ataactttcc gtcccaatta tcaggaggcg aacagcagag
     7681 ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt cttttatgtg atgaaccgac
     7741 aggcgcgctg gattttgaaa cgggcaagca agtattgaaa ttattgcaaa acgctagcag
     7801 acaacacggc aataccgttt tgatcattac acataattct gctttagcgc ccatcgctga
     7861 tagagtcatt cacattaatg atgctaaagt aaggtctgtt gaattaaatg atcatccttc
     7921 atccattgat gagattgtat ggtaggggga ttttatgaaa acaagaacat atcttaaagc
     7981 aggttttcgc gaaattcggc aatctaaagg acgattcatt gcaatcatcc ttattgtcat
     8041 gctaggaaca cttttatttg taggtgtcaa aactgctgga cctgtcatgc aaaaaacgat
     8101 ggatcactat gttggtacag caggactttc tgatttgcaa atcgtttcta ctggtggctt
     8161 gacacaaaaa gatattgctc aagcagaaaa gatcccagat gcaaaagtag aaacaggaaa
     8221 acagatctat tattccaata ctggcaaaaa cgaagtgatt caaatatttt cttataacaa
     8281 aaataacaag cagaacaagc tacaattaac tgatggaaaa ctcccggaaa aaccaaacca
     8341 gcttgtattg gatgaaaaag cggaagatga agggtataaa atcggtgata tttatcagat
     8401 tgattcagat gaattgaaag aaaaagagta taccatcacg ggctttgtcc gttctccatt
     8461 gttcatcaac aatttggaga gaggatacgc gaacgtcgga aatggtagcg tcgattattt
     8521 tgtctattta cctgaatccg tttttaaaac agatatttat tctgtcttat atcttgattt
     8581 tacaaacgta aagaatttga atacgtacag caaagcttac aaagataaaa tggagcaaaa
     8641 tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa gaacgcttag aagagctgaa
     8701 aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa atagcggatg gcaaagcaca
     8761 aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa cagttagcgc aacagtcagc
     8821 aactatccag cagcttcctg aagcgcaacg aacaatcgct gttcaaacat tatctcaaca
     8881 agaagctcaa cttgctgaac aagaaaggca attagttgag aaagaacgtg aactggcctc
     8941 tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa aatttaacta aaccatcata
     9001 cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa tttggtgatc tgtctgatcg
     9061 tatcgctgcg attgccaatg tttttcctgt tttctttttc tttattgccg cattgatcac
     9121 ctttacgaca atgacacgta tggtagaaga aaaccgcagg gaaatcggga cattaaaggc
     9181 gttaggttat acgaaagttg aaatcgcaaa aaaatatgca atctatgcaa gtctagcctc
     9241 agggataggt attgttttag gaacgatatt aggaacgaat cttttgccaa ggatcatttt
     9301 tgagctgtct aacgagcggt atgatattgg gagtgcgctt attttttatg actggccgcc
     9361 gattttacaa gcagctgccg cattcttcat tgccgcattt ggcgctgcaa tgatcgtttt
     9421 attcaaagat ttaagagaac ggcctgctgc attgcttcaa ccgaaagcac cgaagccagg
     9481 gaaacgcatt tttcttgaat acatcactcc gctgtggtca cgccttagct ttaatcaaaa
     9541 aatcagttac cgaaatctct tccgatataa gtcaagaatg cttatggcga ttatcggaat
     9601 tgctggatgt gccgggctga tggttgcagg cgtgggattg aaagattctc taagttctgt
     9661 ttcagccaaa caatttggaa cgatcattga ctatcaagca atcgttacat tggattcatc
     9721 cacggaagat agtcgtcaaa aagcagcgga tatgctgcag aagcaagaaa aagtaaatga
     9781 gatcctagct attcatactc aaaccgtaga attgagaaaa aaaggacatg cttcgcaaag
     9841 tctgacagtg atcgtaccaa gtaaaacagc tgatttggat cgttttatcc atttaactga
     9901 tcttgaagag aacccacgct ccttgcctaa aacaggaatc gctattacgc aaaaagtagc
     9961 agaactattt gacttatcta caggagatca tatttcgctt tatgatgaag atcaaaaaga
    10021 atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta ggtagcttta tttatatgtc
    10081 ccctgaatat tatgcttcag ttactgatca atcgatgatg gataatgcat ttttgctgcg
    10141 tacagataag atgacaacaa aagaagaaga aatgctttcc gagaaactgc ttgccacaga
    10201 cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa acgcaagaag aatcaatggc
    10261 gaacttagac tcggttgtct taatctttgt cgtattatca ggactgctgg cttttatcgt
    10321 cttgtataat ctaactaata tcaatatttc cgagcgtgta cgcgaattat ctacaataaa
    10381 agtccttgga ttttttgata aagaagtcac gatgtacatt gttcgagaaa atatcatctt
    10441 tactctttta ggaatcatcg gaggatttgg tgtaggatat atattgactg acttcatctt
    10501 gcaacaagcg tctatggaga atgtcatctt tccattagtg atcacatggt gggcttatgt
    10561 cttatcagca ggattaacta tcgtatttac ggttattgtg atgattgtta cccatttcaa
    10621 attaaagcat atcgatatga ttgatgcgtt gaaatcgaat gaataaaaac agaggttgtg
    10681 aaatcagcgg tttgacctga gtattagaga aacaaacggc aaaatagttt ttcatatttt
    10741 gccgtttttt gagctaatac cgaaggacag ctgattgaac accatgaatc agaggttgta
    10801 aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata aaattagctc ctcatgtttt
    10861 acatttgaga agactgattg ccgtagataa aagcttttga acaccattta tagaagaaac
    10921 aagaatattt tgctatttta aacaagagga tagacaaata catgtctgat ctcttgtttt
    10981 ttattgggca acagacctct tagaatgtat aaagataaat tttacaaacg ctttcggttc
    11041 agttataata ataaatgata acttgtatat ataagttgtg gtaggtaata aatcagttga
    11101 atggaggtaa atatccatga catttaaaga caaagtcatt taccaaatct atcccaaatc
    11161 ttacaaagac agtaatggtg atggaattgg agatttaaaa ggaatcaaag aaaaactccc
    11221 gtatctgaat gatctaggca tcgacatgat ttggctaaac cctatttatc caagccctca
    11281 aaaagacaac ggttatgata tctctgatta cacagccatc gatcctatct ttggtacgat
    11341 ggaggaattc gaacagctaa tagccgaagc aaagaaatac cggatagaaa tcatgctcga
    11401 tatggtgctg aatcacgttt cgacagaaca tgaatggttc aaaaaagcac tagcagggga
    11461 ccagcattat caggacttct tcattttaag agaagagcca acggattggg tatcaaaatt
    11521 tggtggaagt gcttggtcgc catttggaga tacggggaaa tattatttgc acttatatga
    11581 tcggacacaa gctgacttga actggcggaa ccaagcagta agagaggaac tattcaaagt
    11641 tgttcgtttt tggatggaaa aaggggtaaa aggattccgt ttcgatgtta tcaatgttat
    11701 cggtaaagac gaacaattga aaaataataa agaaaatgat ggcaaacctg aatatacgga
    11761 caaaccaatc acccatacgt atctaaaaga attgaaccaa gcaacgtttg ggacggattc
    11821 cactatcatc acagtcggtg aaatgagctc tacgacgatc gaaaattgta tcttgtatac
    11881 agaacctagt agagaagaat tatcaatggt ctttaatttc catcatttga aagtcgatta
    11941 tgaaaatggg aataaatggt ctactcctcc atttgatttt gcagaattaa agcgactatt
    12001 tcatacatgg ggagaaaaga tgagtgaggg taacggctgg aatgctctat tttttaataa
    12061 tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc gatcagtatc gaaaacaagg
    12121 agcagagatg ctagcaacca tgatccattt gaaccgtggg acgccctata tttatatggg
    12181 agaagaaatc ggcatgatcg acccagacta ttcatcgatt tccgactata ttgacgtaga
    12241 aagtttgaat gcttataagt tactccttga acaaggattc acagaaaaag aagcgtttcg
    12301 tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc atgcaatggt cagacacaga
    12361 acaagcagga tttactacag ggaaaccttg gttaaaagtt ggaggcaagc tagaagagat
    12421 taatgtagaa aaagaactat cttctgaaga ttctattttg tcctattaca agaaattgat
    12481 tcggctgaga aaaacatatc caatcgttgc acaaggagat tatcatgctt atgaagcaaa
    12541 tcatccggaa gtttatggct ttcttcgcca atttgaagga cagcagctgc ttgtattaac
    12601 aaatttctat gataaagata caaggatcgc catcccagaa ggattcacag aagccaatat
    12661 tcttatcgat aattattccg atactgttct tcatcaaagt ctggagctaa aaccatacca
    12721 ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa cgtcgttcct acgagctatt
    12781 ctcgtaagat cgacgttttg tgttttattt aaaataatct tcttcagaaa atgaagagtt
    12841 ttcatactct cctttatcgc cataaaattt attgtagcgt tgtttgaatt tacggaaaat
    12901 aaaagtaacg agtaatttga ctaacgtata gataggaata ccaaaaataa cgcccacaat
    12961 tcccattaga tcacccatga caagtaaaac gattaagatc gtaatcggat ggatctgtaa
    13021 acgatcaccc attactcgag gaatcaccaa gtcaccgtgg agaagttgta cggcaaacca
    13081 gacaatgaca aatttgacag ccataaaagt agagtcttgg aatgcaatga tcaaacctgg
    13141 aaagaaggcg ataaacggcc caatatatgg aatgatacat aacaacccag cagaaatagc
    13201 taaaacacta gcaaattcta atccgatcag taagaaacaa acccagtaga ctgctccaag
    13261 gataaatgaa gcaatgattt gcccctttag aaatgaaccc aattgttgat tagcaatctt
    13321 tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca atcgctaaaa ctgactgata
    13381 aaatttttga ggatctctca aaaggaaaaa agcgacaatt ggaccagtaa ataaagtaat
    13441 aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt tgtgcgccac tttcccaata
    13501 atttccaata aatccagcta tatcagacgt cacgtcgtca atagaagcga aaaattgatt
    13561 aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg agactctctt gaaaatcttg
    13621 aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt tcttcaagaa aaggaataag
    13681 ccatatgctt ccaagcccca ataaaaataa aataatcaca taaacgataa tgactgacaa
    13741 gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt tccaaaagat aatacaagat
    13801 cagtgcaaac aataatgggg gcaagatagt agcgataatg attccgacag gcttgaatat
    13861 aaacgagatc tgatggaaaa aataaatgat cagcgcaagt aagataacta ttcctaaaac
    13921 atagtaagag gtttttcctc ctaaaaagcg gatcaaacga ttcgttgtat ctgtttcttt
    13981 tttcactctt tttcctcctt tatctataac ttcattatag acaaaagcgg gtataaaagg
    14041 aaaatgatag aggttgattg caaaaatatc gtcaatcatt catataaact ttctcatcaa
    14101 ttaaaataag aaaacgcttg acagttcttt taaccatatg ataaattaaa tcctgttgct
    14161 aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac attcaaatga aagaagatga
    14221 gaagaggagt gtcataaatg aaacgattga taggtatatt ggccatgctg gttttagcag
    14281 gtatgttaac tgcttgcggg gcaagtggga aagccgagga cagtaaagaa aaattgagcg
    14341 tgatgacgac cttttatccc atgtatgatt ttacaaaagc aatcgtcggg gatgaaggag
    14401 aagtagagtt attgattcca gcagggactg attcccacga ttatgaacct tctgctaaag
    14461 acatggcaaa gatccaagat acagatatct ttgtttataa tgatgaaaac atggaaacat
    14521 gggtccccgc aatccaaaaa acattacaag aaggcaatgt ccatacgata aaagcgacag
    14581 aaggtatgct tttacttccg ggaagtgaag aaggacatga tcatgaccat gaacacgggg
    14641 aagaaggcca tacacatgag ctagacccac atgtatggct agctccaagt ttagcaatca
    14701 agcaggtagc aaacatacgt gatcagctga ttgaagctta cccagaaaaa caggaagtat
    14761 ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc actccatcag ctttatcaag
    14821 aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca acatgctgca ttcaactatc
    14881 tagcgttaga atatggattg aatcaagtat cgattgctgg tctttcttct agtgaagaac
    14941 cgtctgcggc gagaattgct gaactgaaac actttgttaa agaacatgga atcaactata
    15001 tttattttga agaaaacgcc aaagatagta ttgccagaac gttagcaaac gaagcaggtg
    15061 tatctttaga agtattgaat cctttggaag gattgacgaa tgaacaaata gaaaatggag
    15121 aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt gaaaaaaacg acagagacag
    15181 aaaacccgct agaagatact ttgaccccca aaaaagaaaa gagcgtctac aacggttatt
    15241 ttgaagacga ggatgtacaa gatcgctctc tttcagattg ggcaggaact tggcaaacag
    15301 tagatacgta tgtagaagat gggacttttg atcctgtttt tgaatataaa gaaaaattga
    15361 atcaggataa gacagcacaa gaatataaag aatattatac gaagggctat caaacagata
    15421 ttgcttcaat caagatcagt gagaaagaga tggcatttac gtttaagaat ggaacaacga
    15481 aacgttcaaa ttatcgttat gtagggaaag aaatcttgac ctacgaggca ggaaaccgtg
    15541 gcgtaaggta tctattcgaa gcagaagata aggaaagtgg cgcttttaga tatattcaat
    15601 tcagtgatca cacgattgca ccggctgact ccgaacattt tcatttatat gcaggaaatg
    15661 aaagtcaaga agtattgctg aaagaaatgg agaattggcc aacattttat ccagatacaa
    15721 tggatggacg tgaaattgca caggaaatgc ttttgcactg ataggcaaaa aagaaccgaa
    15781 gggtttggat cggctggtca tttagtcggt ccaaactatt cggttctttt tatgaaatca
    15841 atttctttaa tccacggata gaggctggga tggtctcgcc gtttgccaac gtgacttctc
    15901 tggttttttt attaatcgac cgcacatttt gtgtattgat aacataggat ttatgacaac
    15961 ggataaaatt ccgatgcatt ttctcgattt ctttgatctt ctcatagaat tgtaattggg
    16021 catgagtcaa atggacttcc agtttatgtg gcgctcctag cgaagtagaa aaatattgga
    16081 tgtctttgat cggtaaaaat ttgatctcat cattaaactc tattttgaag atatcttttt
    16141 ggatttggga catttgaaaa tcttcgaaaa ttttgctcag gatatgctcg acattttctt
    16201 ttaaatcata aagatcttct ttgacgatat aatcagtcgg ttcgatgttg ctttttaaga
    16261 ttttcatcga catttccgta tgggaagtca catagattat ttttcctaaa ggatctaatt
    16321 cacggatcaa tttaccgatc gctacaccat ccatttcact gtcttctagt tcgatatcta
    16381 aaaagtaaat accaaaagaa gaagttctga tcttggctgc attgagaagt tcttccggat
    16441 cagaggtaga aatatggaca taactgtcta agttttcaat catgattcta tttttgatca
    16501 ccgttgataa aacttctaat tgttttggat cgtcttcaca aatataaatg ggaatcatat
    16561 cattactcct ttattttatt tgtatataaa tcgtttgaga aaaacgctgg ttttcaattg
    16621 atgtttcaag gaaaagataa ggattggtct ttgtcaattc atccaagata aacaagccaa
    16681 ggccttcgtt ttttggattt gtttttgttg agaacccttt ttgtttcaac tggtttaacg
    16741 gtaatgtatt tgttgttgta ttgatcacac gaatcatcac ggaatgattc ttcttgagta
    16801 tggtcaattc gatttttttc ttctttgcag cactagctcc ttcaatcgca ttgtccaaga
    16861 taataccaag catacgtaca aggtttaccg tatgtttcgt atccagttcg attggctcag
    16921 gaaaatgaag ctggacgatc aacccttttt cctgtgccat catgagtttt aatgacagta
    16981 aactacggat ctcaggaact ttcatattat ccaacatact tagattggcc ggaaaaaggt
    17041 tcatcatctg gcgtgtaggt aatatcgttt gatcataata agaccgtaat ccttctaagt
    17101 cgccttctga aatatagctg tgaagagaaa aaagcagatt ttggtaatca tgtttgaatt
    17161 ctctcaagtc ttgatagttt ttttctagat ctttgatata atcctgttgc atcctgactt
    17221 gttgctctaa taactgatca gctaatttct tattagagat tttccaataa taaaccaaga
    17281 tgatcaggcc aacacacaga tatagaatga aaaaactcgt attcaaagct aagacatcag
    17341 gtgtttcacc agaaaaacgg gtataaaaaa taatgatata gtatgtaaat aatatcaatg
    17401 tcccgatcgt accgatcata cggcggtcca cagtgtgaaa ccaagtctct aacgattttt
    17461 ttaaacaaaa agctaataaa gcagataaaa aagcggataa gacaatgtga aaatactgca
    17521 atgaatctcc tggttcaata accgaagatt tcaaaacaaa aaaatcgccg atgcttgtta
    17581 agtgatctcc taaaagcgac gtcaataacc caaaaagaaa gcgcgtaaac caattgttga
    17641 cgcgaagtcg aaaagaagaa aaaagtaagc atcagcattc ctatagcaat tagagcagta
    17701 aaaatcccta aaatcgaata gacccatcca atcaaaatac aagaaatcag aatgactccc
    17761 gcaaacagat atttgtatcg gatgtgatgg tcaatcaata agatacaaat tgtgtaaatc
    17821 aaatattgca acgctatgga ccataaaaac ataaagtaca gctcccattg atttttacca
    17881 aattatagca caattggcgt attatttttt taatttttca gggattttga attcagaact
    17941 cataccaaag cagcagccag atacaaaaaa gtcagataag ttagttaatt gtttaatgat
    18001 tgtttttttc atgaaagatt cctccaaaat tcatcaagta gtttgtaata ggaagcaacg
    18061 tagctatttc taaaacaatc ccaagtagca aataggtaga aaagtaagaa gctgagaaaa
    18121 ataacgtaat gagagaaaaa attagaaaca tgctgatcgc cttctttaat aatgggggac
    18181 gtttctcggg ttcaatggga ttgattgctg tacctcttga tccatagcga agcagtaaca
    18241 aaaagcagct gattaacaaa aagaccatca ttgggaagga gatagtcaat tgcagatgtt
    18301 tcatcaccaa gggaacgccc cataaataaa gtagtgtcca cagcagacaa acgaattcag
    18361 agtcggaatg caatccataa gcatatctgc ggatgcagat ataagaaaga aaggtgattc
    18421 ctgtttccgc ccatgagcct gtcaccagag aaaagactag aacgaccgct agttttccaa
    18481 gattattcaa taaaacttct aagacaaatt ttacttggac atacaccata tcttcttccg
    18541 atttgtctcc gctgaataag gcttctaata atttgttcac aactttatct ttcatctcat
    18601 acgtacctcc accacaaatt ttatgctaaa aaaaataaaa aacgtctttt gtttcataaa
    18661 aagtggtttt gacatcaaaa ccgtcatgat ctcctaaaaa aatcttcgtt ttccaagaac
    18721 cttaccaaaa aaagtgacag ttttgatacg aaaagtactt tttatgacaa gtttgtgctt
    18781 tttcgagtga tttatgttaa aaaattactg accttataaa aggtgatgga gggattctta
    18841 tggaaaatga acaattgttt gaagtcggcg aaacatatgc atttaaatct gatttatttg
    18901 aatcaccaat aaaaggaaaa atagaaaaaa tctatgaaaa tagtgtgatg atacgtgtca
    18961 tgaaatgcgc agtagaagac aatgataccg ctcataatct gcactggcag ttagttgtac
    19021 gcaaatcaag tacaatagaa gaataaaaac agataaaatg ttgagagcat aaagaggttg
    19081 tgagtctccc gtttagctct gagttacaag gaataatgtt aaaaaacagc ttttcatgtt
    19141 ttatgaatat tgccttgtga ccgaggtgtt ggcaggagaa tactgtttat ttgaaaaaag
    19201 ggctgcgaca agacttttgt cacagccttt attttcatga ataggagtta cttttggcta
    19261 cttttagaga agtagctttt aaggagcaga taaagtcaag gcttttattt ctataaatga
    19321 agtagtaaaa tgaagaacag attttattac aggaaggatg ccgataattg tgatcattgt
    19381 tgcaggaacg atcggtgcag ggaaaagcac actaactgaa atgcttgccc aagatttaga
    19441 aacaaaacct ttttatgaga acgtggaaga taatgaagta ttgccgctat tttattctaa
    19501 tccagaaaag tatacttttt tattacagat tttctttttg aacaaacgct ttttggcaat
    19561 caaagatgct ttcagccatg atgacaacgt actagaccgc tccatctacg aagatagtat
    19621 gctgtttcat ttgaatgcag atttagggcg tgtttctgaa gtagaagtaa agcagtatga
    19681 aggtctttta gaaacgatgc tgaaggagtt agaagaaatt tcgccacaaa agaaaccaga
    19741 tctactggtt tatattcgtg tatcctttga aactatgtta gcgagaatca aaaaaagagg
    19801 ccgggaatat gaacagctag aacaagatcc tgaactctat tcctactata aagaactcaa
    19861 tcgacgatac gaagagtggt atgaacagtt tgatatatgt cccaaaatag tgatcgatgg
    19921 ggacaaatat gatttcgtag ccgatccggc atgtggagaa caaatcgtac aagaaatcaa
    19981 gatgcgggca aagaaaatga cagaagaggc agatgcccag tcgatccgct acactacaaa
    20041 taacccgaaa gcaaagggaa tctttacaac taaataatat aaaaactaag caaattgctc
    20101 actcgcagat atttctctgt gagcagcatc ttgcttagtt tttttgattc attgagatca
    20161 aatagtgaac cgattcgtat gataaaattg ccataaatga aatgtctgag tgagggccat
    20221 gatcaaaaat aaaatggaaa cgatcaaaat cgaatgggcg ccaaacagat gatgacagaa
    20281 aacaaggaca atagctcctg cgataaaggc aagaacgaca cttagatagt ggaaaaatcg
    20341 atcaaaatct gctttttctt tagaaataca ataatcatat aagttcgttg ctaccccttt
    20401 taaatttcca gttgtaaata aattagtgta ggcacgcccg ttgattttat caaaagctac
    20461 ccactggatc gatgcagtaa aagcaatcaa aatcgttact aatgcgggat gctcatggac
    20521 gggtgtcaaa ctaacaaaga aaaaaatcac tgcttcataa tatagaacaa acaagcgcca
    20581 gaaatgtttt tctttttttc taaaataatc aattagatac tttgttagaa gaatccctaa
    20641 gaaaaagaaa agggtagaca atactttttt accaacactt agccattctc cgtcaaatgc
    20701 ttggatgatc gatagaatga tattgccagt ttgagcagag gcaaaagcac catattggat
    20761 ataagtataa ctgtccatgg ctcctccgat aaatgtcagc aaaagtccta ttgtacgccc
    20821 ctcgtgaaag gaagtctgca tttttttcat caccttttat gaatattttt gtcttcctta
    20881 tcttagcata aacgattgaa aaaaaggaaa aattttcaga aaactaaaat catatcgctt
    20941 cccattttca tttttagaaa ggtctgttag tataaagaaa aaatagacaa cgaaacttta
    21001 aataaccaac ttgaaataaa caaattcaaa gaagaaagat gggaaggaaa tcaatcagtt
    21061 gaatgaaaaa gaaattctcc gattacaaaa agaattgaaa cgctgggaga acaagtataa
    21121 aaagcttcaa caagtctttc ggctacaaga agaaatcatt gctacctatg aagcggctta
    21181 tggcgaagca cccggactta ttgaggaaga agtacaagta aaagaaagtg aaggaaacag
    21241 ctctttcttt aaaatgatgg agaaacgttc ctataatcgc ttcaatcaag aacaaaaagc
    21301 agctatcaca tatgatatga agaaacatct tcgaatcata gcaggagctg ggagcggcaa
    21361 aacgcaaaca atctgtgcaa aagctgttta tctgatgacg caaaaacaag tagacgaaga
    21421 acgcatacta atgatcactt ttacaaggaa cgctgcaaat gaactaaaaa aacgagtaga
    21481 taatttcagc cagagaaaaa ccgctgtcca tatcggaaca ttccatagta tcttttttag
    21541 gatatacaat gaaatttgtc gaaaatttcc tgaagtagca atgcaaggaa tacaaggaga
    21601 cttttcaaag gatactgcac aaaaagtgaa tgctgttttg caacagctga tacgtaaata
    21661 taatctttat atatttgatc aatatggaga aaaaacgatt gcttctcggt tagactattg
    21721 gcagaatatg aacctttcag tagaagaaat ggttcagtta gttaaagaaa aatatgattc
    21781 cattgataag aatgcacgac agccgatatc agaacgactg tatggattgc tcacggaact
    21841 ccaagaacaa aaaagaagcc agcaattgct agagttcaat gatgtactcc aaaatctgaa
    21901 aagcgctttg gagaatgagg agatccagca ttatatcggc caaaaatatg attatctatt
    21961 cattgatgaa tttcaagata ctaatccact tcaatgggaa atcgtgaaac ggatgacaaa
    22021 ggaaaacagg atcaagttga ttgttgttgg agatgatgat cagagtatct atggattcag
    22081 aggatcagag ccagcataca tcaagaactt tgaaaaagaa tatcctacca aaactctttt
    22141 cttattaact aattatcgtt ctcgggcagc tattgtccaa ggagcaaata gattgatcag
    22201 ctataataaa aatgatcgga taccaaaggc gatgattcca gcacaaaaag aagcaggtgt
    22261 tatggaagcc tatttgtttt ctgatactaa agcagaaagt cagtggctaa tcaaacaaat
    22321 tcagacattt attcaaaaaa atgggaaata taaggaatca atcatattat accgatcagc
    22381 ttcacaaacg cagcagcttg tccaatctct gctgaaaacg aatattcctt ttgtactgga
    22441 agcagatagt ccatatgaag gtatctttgg tatcaagtct ttccaacatt tctatcaaaa
    22501 aattgttcat tggcaaacag cactgaatct gcaaaagaaa aatcaggcct accagcaatt
    22561 gctccggcag ctgatggcag actgctacct gaaaaaaagt gaatgcgatc aatacttttc
    22621 tcctaaatat caaaatatat ccgttgcaga ttatattttg tcgataagcc ccaatttaaa
    22681 atcgaaatct gcagaaatcc gagagtttga ggaagcgttg cgtaaaacag accagcatca
    22741 agaaatctat tcgcttgttc aaagctatct tcatctgcca cgtatagcta aggaaatcga
    22801 ccagagtgat caagaatgga tacgagaaga agtaaagcag catgctactt tcagaagact
    22861 ccaagcctta tcgcaagaaa cccaagcgac agctaaagaa ttaaaaaccc gcttgattgc
    22921 ttacagacaa ggaaaacttg atgcactatg tatccagagc attcacaaaa gtaaaggtct
    22981 tagttaccga aatgtctttt tgattggttg taacgaagga agtttgcctt ataacggagc
    23041 gacagaaaag aaagtaatcg actttaatga aataaaagca gaaccagtaa cgacgataga
    23101 agaagaacga agactatttt atgttgcgat gactagagca agaaatcgtc tgtatctatg
    23161 tgtacctcaa atgaaaaatt caagaaaatt gaaagtatcg agatttatca aagaaaccgg
    23221 gagtcgtgtg aaaaaaggaa agtgatagaa gagagggtgt gacagaagtg gtcagtttca
    23281 agaaagaaga aggaatgtac gaaaattgct tctcaaattt ttgtgaaatt tcagcttatt
    23341 ttcgaagaag ctatttctgg aacacagttt attcggaaaa gggaggtgtg acaagaattt
    23401 tgtcacacct cccttattta ttttgaaaat gagaatacta ttatcaaaat agagaatata
    23461 tagaaaaatt ctcattattc aaacgtaaat aatcagttat tttgattttt tttatatttt
    23521 attagctaat aattggtatt ttaaaagaaa ttactgtaga attggattat aattaaaaag
    23581 gagtgagaaa atgataaaag tatatacttc gcctagctgt acttcctgta gaaaagcaag
    23641 agcatggttg caagcgaatc aacttgaatt tgaagagaag aatatttttg ccgaaccgtt
    23701 aacagaaagt gagatcaaaa agatcttggc agcgactgaa ggaggagttc aagatattat
    23761 ttccactcgg tcgaaaattt acgaaaagct gggcatagat tttaacgaat taactttaaa
    23821 gcaaatgatc gtcttattca aagaatatcc ttcattattg agacgaccta ttttgattga
    23881 tgaaaaaaga atactaatcg gatttaatga agatgagatc cggtcatttg tccctcgtga
    23941 aattcgatgt gttgccagaa atctgatcta tttacaaaac aataacatct taaattcata
    24001 gggagggagc cttcgtggaa gtgaaagtct atggatcagc gcactgtgta acaagtctga
    24061 aggcgaaaca atggttagaa caccatcaat tgccttatca atttgttgac ttagaagaac
    24121 gcgatctaag tacagaagaa gcagaagaat tatgcagttt tgaagaagtg caagcagaac
    24181 agctatttgc gacttggtct gaggcttttc acaacattgc gattgacctt tcctgtgtag
    24241 gaaaaaaaca actgattttt ttatgcacac aacataccaa tctcctcaga cgtcctttga
    24301 ttattatcga tgaagttcta tttattggat acaatgagca gttgatggaa cagcttatct
    24361 taaataaata gactttaact gttttaatca agtcactgag catttcatac agttgtaaca
    24421 gtcgaacact tagcatcaag cgtgttatgt gttctttttt tgactttatt tttctactat
    24481 agagacatgc tcaatttatg tggtcaaaat tcaaatctag gtggggatat agagttaaaa
    24541 atatattgac atgtgtaaat ttacatgata tattcatttc tgttgtttta agtagcaaag
    24601 caataaaata gtattttaaa aaacatataa aatagttgtt gacatgttag aaatgacatg
    24661 ataagataac tgagttgtta aaaaagaacc acttgtgaag cttgattcta tcgaaaaaaa
    24721 cttcaaaaaa gttcttgaca aataacaaat gataagttat gataaatgag ttgctgtaag
    24781 atttcgaaaa acgaaatcaa aaaaactttt aaaaaagagt tgacaattac ttgtctctct
    24841 gatatgatat aaaagttgct acggcagcca aaaacgaatc ggggaacact cctgatgaag
    24901 tagacctttg aaaactgaac aaagtaagac aaaccaaatg tgtagggcgt cttgattcaa
    24961 ttcaagacaa caaacatttt ttaacaagca agcaatatgc tagcaaacaa attgagctta
    25021 acaatcgcaa gattgttcga actttttatg agagtttgat cctggctcag gacgaacgct
    25081 ggcggcgtgc ctaatacatg caagtcgaac gcttcttttt ccaccggagc ttgctccacc
    25141 ggaaaaagag gagtggcgaa cgggtgagta acacgtgggt aacctgccca tcagaagggg
    25201 ataacacttg gaaacaggtg ctaataccgt ataacaatc
//
LOCUS       DAFBQH010000032        24666 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.032, whole genome shotgun sequence.
ACCESSION   DAFBQH010000032 DAFBQH010000000
VERSION     DAFBQH010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.032"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            236..1042
                     /locus_tag="LK694_001478"
     CDS             236..1042
                     /locus_tag="LK694_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5052349.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            1054..2274
                     /locus_tag="LK694_001479"
     CDS             1054..2274
                     /locus_tag="LK694_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5052350.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2264..3850
                     /locus_tag="LK694_001480"
     CDS             2264..3850
                     /locus_tag="LK694_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK5052351.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            3889..6027
                     /locus_tag="LK694_001481"
     CDS             3889..6027
                     /locus_tag="LK694_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK5052352.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            6315..7706
                     /locus_tag="LK694_001482"
     CDS             6315..7706
                     /locus_tag="LK694_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBK5052353.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            7765..8574
                     /locus_tag="LK694_001483"
     CDS             7765..8574
                     /locus_tag="LK694_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK5052354.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            8546..9535
                     /locus_tag="LK694_001484"
     CDS             8546..9535
                     /locus_tag="LK694_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK5052355.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINPKTYFILYKKVLKK"
     gene            9649..10644
                     /locus_tag="LK694_001485"
     CDS             9649..10644
                     /locus_tag="LK694_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK5052356.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILIFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
     gene            10648..11790
                     /locus_tag="LK694_001486"
     CDS             10648..11790
                     /locus_tag="LK694_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBK5052357.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQLYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSKK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNFMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELIERDIK"
     gene            11787..13214
                     /locus_tag="LK694_001487"
     CDS             11787..13214
                     /locus_tag="LK694_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBK5052358.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIVGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIIIFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEIRKITRK"
     gene            complement(13281..14570)
                     /locus_tag="LK694_001488"
                     /pseudo
     CDS             complement(13281..14570)
                     /locus_tag="LK694_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(14750..15160)
                     /gene="tagD"
                     /locus_tag="LK694_001489"
     CDS             complement(14750..15160)
                     /gene="tagD"
                     /locus_tag="LK694_001489"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HBK5052359.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            15286..16677
                     /locus_tag="LK694_001490"
     CDS             15286..16677
                     /locus_tag="LK694_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBK5052360.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            16810..17757
                     /locus_tag="LK694_001491"
     CDS             16810..17757
                     /locus_tag="LK694_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBK5052361.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(17797..18957)
                     /locus_tag="LK694_001492"
     CDS             complement(17797..18957)
                     /locus_tag="LK694_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBK5052362.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            19122..20297
                     /gene="mltG"
                     /locus_tag="LK694_001493"
     CDS             19122..20297
                     /gene="mltG"
                     /locus_tag="LK694_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBK5052363.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            20433..20912
                     /gene="greA"
                     /locus_tag="LK694_001494"
     CDS             20433..20912
                     /gene="greA"
                     /locus_tag="LK694_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBK5052364.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            21080..21811
                     /locus_tag="LK694_001495"
     CDS             21080..21811
                     /locus_tag="LK694_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBK5052365.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            21808..22875
                     /locus_tag="LK694_001496"
     CDS             21808..22875
                     /locus_tag="LK694_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK5052366.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            22882..23514
                     /locus_tag="LK694_001497"
     CDS             22882..23514
                     /locus_tag="LK694_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5052367.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            23654..24319
                     /locus_tag="LK694_001498"
     CDS             23654..24319
                     /locus_tag="LK694_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBK5052368.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            24347..24640
                     /locus_tag="LK694_001499"
     CDS             24347..24640
                     /locus_tag="LK694_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBK5052369.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
BASE COUNT         8418 a         3663 c         4782 g         7803 t
ORIGIN      
        1 aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt tccaccaaca caaaatatta
       61 tagaacccct tttttggttt attgcaattg tctagcatct tctgtaaaat agaaacgtta
      121 gagaaaatct tgtatcatag aaaattaaga aagaaataag aacgcagctc ggtaccaaaa
      181 atatggtaaa atggcacttg actgtaattc actgaaatcg cttggaggaa cacaaatgtt
      241 taaagatttg tatttattta tgaaagacat ttatcagaat agaaaattac tgctacagtt
      301 ttcgatcaat gacttcaaat caagatatgc aggatcgttt ttaggaatct tatgggcgtt
      361 tatcaatccg ctatttactg tattgattta ttggctggta tttggtgttg gactgaaagc
      421 acaaatgatc gatgcaaaat acccatttat cgtatattta gttaccggga tgattccctg
      481 gttcttcttc tcagatgcat ttgcatcaac tacggttgtt tttcgggaat atacttattt
      541 agtaaagaaa gtcgtattca atattcgtat cttgcccacg acgaaaatcc tatccaatct
      601 gtacacccat ttattcttca ttctgattgg catagctgtc gcgcttataa atggtatata
      661 tccttcagca atgtctttac agctgattta ttatttattt tgcatgtgtg ctttcttgac
      721 aggattgact tggctgacgg catcgatcca accattttta cctgatatca tgcaattaat
      781 tacgatcgtg ctgcagttga tcatgtggac attaccgata ttatggagcc ctaatcagtt
      841 tgattcaggt cttgtaagca tattgaaatt aaatccgctg tactatgttg ttcaaggtta
      901 tcgtgagtca tttttaagtg aagcttggtt ctgggagcat ggaacatata ctttgtattt
      961 ctgggttttc acgttagtaa tgttcgtaat cggctctgca gtgttccgta gattgaaacc
     1021 acatttttca gatgtgctgt aaggagaata gctatgacag aatatgcaat tagactaaaa
     1081 gatatcacaa aatcttataa tatgtacgca aaaccttcgg atcgatttcg tgaagccctc
     1141 aatcctttta agaaatccta tcatgatgta ttttatgcgt tgaaaaacgt gaatgtagaa
     1201 ataaaaaaag gtgagatgat cggtttcgtg ggagaaaacg gatcgggaaa atctacaatg
     1261 ttgaaaatca ttacgggagt actgacacca acatccggca cgatggaaat cgaaggaaaa
     1321 atctcagctt tgctagaact aggatcagga ttcaatcctg agtattctgg ctatgaaaat
     1381 atctatttaa atggaatggt tctaggattt tcaagagaag aagtcgatga aatggtcgat
     1441 gatatcattg aatttgctga tattggcgac catatttatc aacctgtaaa aacctattca
     1501 agtgggatgt tcgttcgtct agcttttgct gtagcgatca atgtgaatcc tgatattttg
     1561 attgtggatg aagcattggc agtaggagat cttgaattcc aactgaaatg tatggaaaaa
     1621 tttacagaaa ttaagaactc gggaaaaacg attttattcg tttctcatga tattagttcg
     1681 attcgccgtt tttgtgatcg ttcctattgg ttgaaaaatg gagaagttgt cgaatatggt
     1741 gatacgatgg aagtaacgac gaattacgaa aactttttga agaaaaaatc agttaaaaca
     1801 gtagatcgaa ataaaaacca agtagaacgc ttcgcagctc cagatatcgt agacgtgacg
     1861 aaagccgaat tgttggataa aaataaaaat ccagtggata tgctggaaca aggagaaaaa
     1921 ctatacgtta aagtaaccta tgaagtaaaa gacgatacaa tcaaaaatcc agttttagga
     1981 attgctctgc gtacagtcga caatttttat gtgtgcggac tcaatactct actagatggt
     2041 atcaagatcc cttggaaaaa aggtgagaat atcttttatc tggagtatga aaagttaggt
     2101 ttgttagctg gtgaatatta ttttgacgtt gcggtctttg aagaaaatgc aacggttcct
     2161 ttggtataca agacaaaata catgaacttg tttgtcagcg gttcttatat cggagaaggc
     2221 atcgtggtct tagaccataa atgggaagaa gggactcata gcaatgaaat atgattttga
     2281 aatggaccta gatgaacaaa gcagtgtggg aaaaattgcc gcacagatca aaccaggaag
     2341 taaagtttta gagtttggtc ccggaaacgg tcgattaacg aaacatttga tcggagcaaa
     2401 acaatgtgaa gtgagcattg ttgagctaga taaggaatta ttcgattttg tcagcgaatt
     2461 tgcccaagat ggattttatg gagatatcga aagttttgaa tgggcaaact attatgctgg
     2521 acagacattt gattacgtct tgtttgcgga tgttttagaa catttagtag atcctggaaa
     2581 aacattgaag aaagtcagag aatttttgaa tgaagaagga gagatcctga ttacattccc
     2641 taacttagca cataattctg tcatgatcga tttgttcaat aatcaattgc cttgggcttc
     2701 ttatggtctc ctagatgaaa cccataattc tttttatact catgatggtt tccaaaaagt
     2761 ttttgaaaaa gccggattat tcattaatat tgaagattac ttgtacttgg ctgtaggaga
     2821 tacagaatta aaatcaactt atgaagagtt gccggaagct gtacgctatg atttcaaaat
     2881 gcgaccattt ggtgaagtct accaatactt tttctcattg atgaaacatc cagtagtaca
     2941 aacttccatt gcacagccac aaaactctaa ttatgtaaaa gtactagaag taacccagca
     3001 aacgaaacaa gatgaaacaa tccaacaaat accattcaat aatttcacag gggaaaatga
     3061 gacactaagt ttccccgtgt cagaagcaac agaaagaatg gtgtttcgct tcgcacagca
     3121 acctagcttt atcgaattca gtgcagaagc tgctggagaa aaactcatat ttatcgattc
     3181 aaatgctgta gtcaaaacag taaatgattg ttatttgttt gatgggaaag aaataccaga
     3241 gttcgctcta acggatattt ctgggaaaga agtgactatc cattgtcact atcgctttat
     3301 cggggaattg acaccaacaa tgaaagaatt gctggaaaca attcgtccta tggctgaagt
     3361 cacaaagcag ctttctgaga aaaatgatga attagaaaga gaaaatcagc atcttcttga
     3421 ggaaaataca agactagacc atacgttaaa aacaaccaca aatcgctatt gcacattgtt
     3481 ggaaagtgat gaatggacga tcaaacatag actaggacga cgtaagaaaa aaacatcgaa
     3541 aaaaatacaa gaaaaagagc tttctatatg tattgatgag aaaatatggg atgcagaaac
     3601 aaaaatatta aaaatcatcg gatggggaat cgctaattcg gatcgccagc cattgtccta
     3661 taagttatca gctgaccaat cgccattttt cgaagcaatt cctgtttcac gagatgaagt
     3721 caatcaagca gaacagctcg caaaaggaac agaagcggga tttgaattac gaatcctttg
     3781 cgagcaagaa cgttcattct tagtggaagc agtagctcaa aatggtcaat catggatcat
     3841 tgaaatgtaa ataatgatta acttataaag ataaggagta aacgaatcgt gcaaaaaaaa
     3901 gaaattgccg tatttatcga tcaaatcact cgtgaacggg ccactggcga tttgcttatt
     3961 gtgggctggg caattgatga agtgaccaaa gaaatcccta cgataaaggt agaaaaagaa
     4021 aatgtgattg ctgaagcaac acatgtcgta agattggata tcaatcattt atataatctt
     4081 gatgttaaga cccaaagcgg ttttaagata cgtttatccg ggaaaatgag aggaaaagcg
     4141 atcttagatt ttcaaacggc aaaacatcaa aatggcatag ctgtgaaact aaatggtaaa
     4201 tacccttatg atgacggcat tgaatcttca tgggaaagaa aaaaaagatt actgaaaaaa
     4261 gggataaatt atgcaaggac tcatggcgta aaaaaaacaa tccgccgggt caaactggaa
     4321 ttgaaaccag gatcgatcga ctatgcagag tggatcagtc gtcacgagaa acctgatctg
     4381 aaagagcaaa ggaaagaagc acaagggttt gcataccgtc cattgatttc catacttatg
     4441 cctgtctata acgttgaaat caaatggctc gaaaaatgca ttgattcggt attggatcaa
     4501 acgtatgatc actgggaact atgtatcagt gatgatgctt ctactgatcc agctatccga
     4561 aagtgtctgg aatcctatca agcaaaggat gatagaatca aagttgtttt ccgacaagaa
     4621 aatggacata tcagtctggc aactaattct gcgttggaaa tggcagaagg agaatttata
     4681 gcgctcttag ataatgatga tgaattaccg ccatttgcat tatatgaagt ggcaaaagtg
     4741 ctaaacatac atccagaact ggatttgatc tacagtgacg aagataaaat cgatgcagat
     4801 ggcaatcgct ttgatccgca cttcaaagca gactggtctc ccgatacatt gatgggaaat
     4861 aattatatct ctcacttagg cgtatatcga acaagtattg tgaaaaagct tggcggtttc
     4921 cgtaaaggct acgaaggctc tcaagattat gatttggtcc ttcgtgtgac agaacaaatc
     4981 cctgcagatc atatttacca tattgaccga gtcttgtatc attggcggac gatcccagga
     5041 tcaaccgcaa gtaatggtga agccaaaagt tatatctatg attctggagt gaaagcattg
     5101 acagatgccc tgtctagaag gaatatcaaa ggaagcgtac gtcctggcag aatttctgga
     5161 ttttatgaga ttgcttatga cgtcttgcaa gaggatttgg tcagcgtgat cattccaacg
     5221 aagaatggct atgaagatct aaaaacttgt gtggattcga ttatcgaaaa aacgtcttat
     5281 ccaaattatg aaatcattat tgcagataat ggaagtacag atcctaaaat gcaggaatta
     5341 tttgcagaat acaagcatca gctgaaagac cgatttatcg ttgaattaat tgacattcca
     5401 ttcaattatt cacgtatcaa caatctggca gcagaaaaag caaacggcaa atacttttta
     5461 ttcttgaata acgatacaga agtgattgaa cctgactgga tgacagcgat ggtttcttat
     5521 gcacagtttg atcgaatcgg ctgtgtagga gcaaaactgt tttatccaga tgatacaacg
     5581 cagcatgcag gagtattatt aggaatcggt ggtgttgcag gacatgcatt gaataactat
     5641 gatcgaacac attgtggtta tttcggccga ttggtcattg acgtcaatta cttagcagtc
     5701 actgcagctt gtatgatggt caaggcagct gacttcaatg ctgtcaatgg atttgatgaa
     5761 acattagaag tagcattcaa tgatgttgac ttgtgtttga aagtttatga actgggtcgt
     5821 tacaatgtct atgcacatca agcagaattg tatcatttcg aatctaaatc aagaggttat
     5881 gaagatacac cagaaaaaca aagacgtttt gccggggaaa taaaaaaaat gcaagataag
     5941 tggccttctt atattgctca tgacccattt tataacgata atctaactaa agaaggaatc
     6001 ggcgattttt cattgagacc tgattgatta aaagaactgt attttcattt ttattgatga
     6061 aaatacagtt tttttagtat atgaaaattg taacttgtga ccgaagagat gataggaaga
     6121 acacccattc agtaggaaga atgtgatacg atttttgtcc aactctctgt taggcagaaa
     6181 tatacatata aacatacaat agtcaccttt tctagtttct tttaatttat tttacaattt
     6241 ggttattcac taggttttta aactttgcta tggtaagatg atagaggcat tattttatgg
     6301 ttaggagtgt atatatgaat aaaaatgggg agtggaatgc ggcacgtagg atattcatta
     6361 tcatcatgga tgtacttgta ttgaatgtgt cgatctatct tagctttttg ctgaagttcc
     6421 aaggagaagt cccagcaaga aacttagaga cctttcagca ttcagcagtt tttatttcca
     6481 ttatctttat tgcgttgaat attttgttag gagcttatgt attctatagc cgagtgatca
     6541 gtgatatcgt cttcattact gttattggac aggtgctgat agcattgggg atcatggtag
     6601 ttacttttgc tggacgttgg tttgcatttc cgcgtggcgt catcgtgctc tcgtttatac
     6661 ttggcacgat attacttagc gtttatcgga tagttgttta taaactatac ttaagagtca
     6721 gtcgtgataa gaaagtcctt atcgtaggtt tggagaaaga tgtcgcacca gctatccaaa
     6781 actttcaatc taagaaaaac aacaaacata aagttgaagc agtagtgatc gacaagtatt
     6841 acgaaaacac aaaaaaattg attgatgata tcgatattgt ctacttggct tctaaaatcc
     6901 cagaagacga aaaaatcaat atttatcaat tagtaactaa aaaagagaag aagctatttt
     6961 taaatacaac ttttgaaaat ttaacgatga tcaatccgaa catcatgaat ttcgaagatg
     7021 aaagtatcat tgaagcttcc ggtttcaaaa ttcagccgga atatgaatta ttcaaacgtc
     7081 tttttgatat catcgtttcg cttattttac tgattcttgc ttcacctttt atgctaatca
     7141 cagcgatttt ggtaaaaaca acttcacctg gaccaattat ttataaacaa gtacgtatta
     7201 cgaaaaatca aaaggaattt tcaatctata aatttcgttc catgagtgta acagctgaag
     7261 caaaatcagg accggtcttg gcaaagagca atgatgcacg tgtgacacca gtaggcaaat
     7321 ttatccgtgc cgtacgtttt gacgaattac ctcaaatctt taatgtattg aaaggcgata
     7381 tgtcgatcgt cggaccacgt ccagaaagac catttttcgt tgatcagttc aatgaagaaa
     7441 atccttatta ttatttaaga cacaatgtac gtgcagggat taccggatat gcacaagtat
     7501 acggaaaata tgtatcagat tataatagta aactacgttt tgatttgttg tatatcaaaa
     7561 agtattcctt actattagat ttgaagattt tacttcaaac aatcaaaatc ttatttgata
     7621 agatgtcctc acaaggacta gatgaagatg aaaatatccg ttcgttagca gaaatcgact
     7681 tttcacaaga gaaaatttat cgctagatgg gaaaaatttt gagcaaagaa gtaggagaga
     7741 acattggaaa ataaaaatat aagtatgctg ctttctttat atgataaaga aaaaccagag
     7801 tatttaaagg aagcattaac tagtattttt gagcaaacag taatgccaaa agaaattgta
     7861 ttagtttatg acggccctat aaatgaaaaa cttcaagtag ttgtcactta tttccaacaa
     7921 aaagatcctc acttgtttac agttgtaaaa ttagaaaaaa atcaaggatt ggggattgct
     7981 ttagcaattg gattagaaca tacgaaaaat cgcttagtag ccagaatgga tacagatgat
     8041 atcatggaac cccaacgatt agaaaaacaa ttagagatct tccaaaaaga tccagaaata
     8101 gcgattgttg ggagtaatat tgaggaattt actggcacgc ttagcaatgt tatcggaaga
     8161 aggattgtac ctgaatataa tgatgaaatc cgtgtgtttt cgaaaagaag aaatcccttt
     8221 aatcatatga cagtcatgtt tgataaaaat gctattttag aagtgggaaa ctatcgccca
     8281 ttaattggat ttgaagatta ttatttgtgg gtaagactgt tgaaggcggg atataaaggg
     8341 tataatatcc aagagattct agtccatgct agagcaggtg ctgacatgta tgctagacgt
     8401 ggaggaagta aatatctttt accaggaatt aaaggccgat atcgcgtatg gaaagatggt
     8461 ctaggatcac taaaagattt tctgatagtt atttgcggac atcttgtaat tagtttatta
     8521 ccgaataaaa ttaggggaaa agtatatgaa tcaaaactta gaaaaacaaa ctaaactttc
     8581 tataatcata cctttttaca atgcaaaaaa tactttaaaa agagctgtag acagcgtctt
     8641 aacgcaaggt tatcccaata ttgaaatctt gttaattgat gatggatcta cagatggtag
     8701 tagtgacatt ggtgacgaaa ttgctaatct agagagtaaa gtagcatgtt atcacttgga
     8761 aaacgctggt ccatcagttg ctaggaattg tgggataaaa gaagcaactg gaaatttggt
     8821 aggattttta gatagtgacg attatttttt acctgattgt tttaataatg ttttgaaaga
     8881 ctaccatagg aattaccccg atatcattgc ttttggatta agtaaaggaa aaaatataga
     8941 aaatgcaaat ttatttgttc ccgaaaagaa gattggttgt tcaagtgaag aagcaattga
     9001 gcaaatgttt agaagtaagg cagtggattt ttacgtatgg aataaatttt ttaaaaggga
     9061 attattcaat catatagttt ttccagaaaa taaattatat gaggatatgg ttcctatgta
     9121 tgaagctttt agagcggcta gtaaaattga tattttgcca ttttcaggta ttttttatta
     9181 tcaaaataat gatagcatcg tttatcagtc ttttaattca aaacagtatg ataatatttt
     9241 gcaaagaaaa atattagttg atgaagtaaa gaaagattat cctcgactta ttaatttggc
     9301 caatgctcga ttagtagacg gatacctttc aacagggttt aaaattactg gcaagaatca
     9361 aaatcagaaa gaaaagcaaa aatattataa tcagtctaaa agagaaataa aaagtatata
     9421 ttcaaaaata agaagtaaca atgagatatc aattgctaaa aaattagctt tatttttata
     9481 cttaattaat cctaaaactt attttatttt atataaaaaa gttctaaaaa aataaagttt
     9541 ttttcgatat gttatttttt atgtttatta acttcggaag actaaaattg ttactggtaa
     9601 tgatacattt gtaaaaataa aaaagatgat ttttaggaag tgaagagagt gcataaaatt
     9661 agtgttatag tacccgcata taacgtagaa gactatttag aaaactgctt agaatctatt
     9721 tccaaccaga cacattcaaa tattgaaata atagtagtca atgatggttc cccggatcga
     9781 agtggggaaa ttgcagaaga atataaaaag aaagatatgc gattaaaagt gatacatcaa
     9841 gtgaatggtg gtttaagtgc ggctaggaat tctggtcttg attctgcaac aggagatttt
     9901 gtgtgcttta ttgacagcga tgattggata gaaccagact atcttgagtt actatatttt
     9961 ggcacgcaaa cttatggagc agatatctcg gtgctaaaaa taaaaaagat ttcaagttta
    10021 gaacaactaa aagaacatac aattgctgag agaaaatggg aaatatttga tagagaacaa
    10081 gcaatgaaaa acttatttat aaataataaa attggctatt ctgcaaataa taaattattc
    10141 aatagaaatt tatttgataa tataagattc ccactgggta aattaatgga agataaggct
    10201 actacttatt tgctgatcga tcattccgaa aaagttgctg tcaattcatc tgctaaatat
    10261 cactattacc aaagccctaa aagtatactg agaggaagct tcaatcctag aatttttgat
    10321 tcttttgaag tccatgaaga tatactaata ttcatagata agttttatcc ggaattatca
    10381 attaatgtta gaactcgcta tgtctatgaa gcaataagga tgatgatgac aatgattaaa
    10441 aataactata ccaataaaga agattattta cgttctataa atattttaca acgctttgaa
    10501 aaagaagtga aaattgatcc cgacttttca ccaaaattaa aaaaattagt aaagttttta
    10561 ttaataaata aaaacttgcc ttactatatg tcaagtagta agatagcaag taaattcttg
    10621 attaagatag aatctgtgag gtaagagatg aagaatataa aaaaattttt ttcaaggaat
    10681 attttcccgg tttttattaa aatattacct attcaaaaaa atttaattgt attttcaagt
    10741 tttggcgggc gtaactattc tggaaatcca agagttattt cagagaaaat tcatgaaaaa
    10801 aaattgaatt acagaattat ttggttaatc aaaaaagaag cgattataac tgatttacca
    10861 gattatatta aaagaattga ctccgattct tttttagctg ccttttattt atatacagct
    10921 aagtattgga tagacgatag tcggaaaacg attaagtatg caaaaaggaa aaaacaactt
    10981 tattttcaaa catggcacgg aacgccttta aaaaaaattg aatttgatgc aaaagataag
    11041 ttacctaaac gttacctaag ctatgcgaaa aaagatagtg agtcaattac atatttatta
    11101 tctggtaatc gctatagttc taagaagtat gtttcagcat tcaatatcgt tccttctaaa
    11161 atattagaag taggaactcc tagaaacgat gaattagcct ctagtaaaga gcaagtgttt
    11221 gacttgaaaa agaaacaaat caatgtctta tttgctccaa cttttcgaaa taatattgag
    11281 gacaacggaa ttacgcaact agaatggtta ggtgtggata acttacgtga attttttaaa
    11341 aagcaacaac aagaattaaa ttttatgact aaatttcatc ctaatgttca tagtaaatta
    11401 gcaactgatt ctaaaagtat agaatatatg aaaaaacatc aaattacttt aattaatgat
    11461 gggattcctc ttgaaatgct ttttgaagag atagatgttt taatcacaga ttattcatct
    11521 atttttttcg attttgcgct aacaaaaaaa ccaattattc tttttaacta tgatgaaaaa
    11581 gaatatagct tagaaagagg tttttatata cagttaagtg agttaccaat tataagcgta
    11641 caagatagtt atggccttgt tgatgttttt aaaaaccaac aagaaaagct tttgaaaagt
    11701 tctcaagagt tacttgatta tattggaaat tatgaaacag gagcaagtac agaaaaggtt
    11761 cttgagttga tagagaggga tataaagtga aaaaaacagt aaaagatata ttttataatg
    11821 cgatatatca agtctttttg attgttttac ccttacttac tatacctata cttagtcgta
    11881 gaattggttc aactggttta gggatttatg ggtatgtttt ctcaatatca cagtttttaa
    11941 tgactgtcat tgctgtggga atgaatcctt ttcgtattcg aaatatcgct aaatcaagga
    12001 aagataaaaa agcactatct ttacaattct ggaatattta ctttattcaa tttttaatag
    12061 gcttgagtgt ttcctttctc tatatagcct atatttattt ttttgatgta aagtataaaa
    12121 atttatactt aattcaattg atatttattg tagggctaac attagatatt tcttggtttt
    12181 ttcaaggaat tgaagaattt tcgaaagtgg ttattagaaa tacagttatc aaattatcat
    12241 cggtattgtt aataatcatt tttgtttcaa ctaaagatga tttgtggatt tacgtgttca
    12301 ttacttcaat tactaacttt ttaggaagtt tagttttctg gttaagtatc cacggaaaag
    12361 taggtaaacc aatctttaac tctaatgttt ttaaacagtt gtggaaacca gcttttatta
    12421 ttttgactcc tcagttgttt atgcaagtgt acacaacttt agacaaaacg atagttgggt
    12481 attttgtgga tccaacggaa ttatcttatt atgatcagtc tcaaaaaatt gctcgtatta
    12541 tattggcagt cttaacatca attactattg ttatgttgcc taaaatatct aaagcacagg
    12601 cagaggggaa atcagaaaat attttagttt acacaaaaaa atcttttgat tacacgatta
    12661 tcattgcatt aattcttttt agtgtggtgt ttatcaatac aaaagaattt gttccatggt
    12721 tttttgggga gaatttcaaa ccgatgacaa taaacatgct tatttcttgt ctaattatag
    12781 ttgttagtcc aattggtgga gtttttgcta accaatttgc aattgctata gaaaaagata
    12841 aggagtatgc ttatccaatg atcattggtg cgattgtaag tattattgga aatgtagctt
    12901 ttgtgcctac ttatggcgct gtagcggcta cgatcgtact cgtggtagta gaattattag
    12961 tctttttctt tagagtattt tttgtacgaa aagaaataaa aatatcctat ttctttggga
    13021 agaacgttat cttatttttt atagtcaatc ttactttgac tacttgggcc tattactttc
    13081 ttccacaatt attctcttct tcatttttag atatggcaat taaatcaata atgattttca
    13141 ttatttatct tccgtttgtt tattttcttt ttccagatat acgaaatgaa ataagaaaga
    13201 ttactcgaaa ataaaataat gttgtaaaaa agtagttctt tgtcaaatcg ggctgataag
    13261 gtcatttatg aacaacaaag ttagtaagag ttactagctt ttaatgcata gctgattaac
    13321 ctgttgatta gaaaaatcca caagcgcata ttggtaaaac tgcgaaaccc ataagcaaat
    13381 cgtttcaatg ttttgatatg tgtgcgaaga ttctctaatt ttgttattag aatacttttt
    13441 cgaaaatgct agttaaaagc ctttttcaga tatgaaaaag gctttttgag atagtgaagt
    13501 ttcgagggaa cttctttggt cgtttctcga agtagctagc tgtttcttgt gataattgct
    13561 aagcagcttt tgataaactg cttagcaatt ttaagttcat aagagattga gggtagacga
    13621 tcaactactt cactttctgt taaatagcgc cattgaaaat tctgccattt cttgaatgtt
    13681 gtgtaatcca actcgtcatc tgattttaga agtaatttat acaaaggttt caattgtcga
    13741 tagtacttag cttgtgaagt gtctttttga ttcaactgct tcatcacacg ttttcgaaca
    13801 cgatcgaaaa acttgttcaa gtgttgtcca atgtgaaagc aatcaatgat gatttgggcg
    13861 tttggaaagg actctttgac taatgaaaaa taaggggcat tcatatttgt gacaatgtat
    13921 cgaaccccca tatgagcttt tcttgtaaaa cgcatgaaat aagcaatcaa atcttttttt
    13981 ctccggctgg acaaaatgtc aaagagcttc cctgtctctc tattcataca agaaaaggag
    14041 aactttccat gggaaattga aagagagcga aactcgtcga atagtaaaca atgaggtaaa
    14101 tagtctagca tctgtcgcag cattgccgat agagcagata atgtccgttg aaatgtcgtt
    14161 actgaaagat cacaacgttt cgtaagtgtc tttaacgctt gacgctcggt caactcttct
    14221 aatatcgtgt aatgaacttg tttcgaaata aaacatgaag gaacgacaag atacgtatga
    14281 gcagtaaaat attttccgca tgattggtag tggtaatgtt gttttctaag acgcaaatag
    14341 gtttatttat tcccactgcg atttaagata atcattgaaa tcttcttgcc gtttttcacg
    14401 agctgcttcg cagtttcttt acaaaataga caatgtctaa aagtgcgaac taatgtacct
    14461 tcgattacga tggctttctt gtccttataa aaagtttttt gaaaagaatt aatttcaata
    14521 tttttatctt taatagtgaa gtgctctttg atagaacgag aaatggatat acaaaatctt
    14581 cctttcagat tgatgtttta gacgactcta ttttatcgaa atttttgtat gtctgttttt
    14641 tttacaagaa aatctaggat cgacagaaaa ttctatcagt cctagaaatt atagagccat
    14701 ttttttacgt cttatccaga tatattgttc tttcattgtt taaaaaatat tatttatcac
    14761 tatttaaaga attaagttcg ttctttattt gagttgtaga gatctcaggt gttcttggta
    14821 aataaattac ttgtgcatct gttagttgtt caagttcatc aaattgtcca gcccaatcat
    14881 cgcccatcac gaaataatct gcatggtatt cttttatatc ttttactttt tgttcccaat
    14941 tttcttcagg aataactaaa tcaacgtagc gaatagcttc aagtaactgt tttctttttt
    15001 cataactaaa ataacatttt ttttgtttcg cattccaatt gaattcatct gtagaaaggg
    15061 caacaataag ataatctcca tattgttttg ctcgtttcag taaattgata tgcccgtagt
    15121 gaagcaaatc aaaggtaccg taagtaatta ctcttttcat ctttcacctc tcgaaattca
    15181 ttaatataca ttgttagttt agcatttttt tgttagaatt gaaagaacca gtttatccta
    15241 aaaaggataa gaaatgttta tgagaaatag gtggatggag gaaaaatgtt gaatgaaaaa
    15301 tttttaaaat atgtgaaagg ttggtatttt atttattttg gttttattct aatgttacga
    15361 gcaataggcc cgtatttact attacctact agatatgata ctttactttt ttggcctgct
    15421 ggaatagcgg gagttgtctt gatcattata gatgcaatta ttgcatttag agaaaaaaat
    15481 cttaaaaaat atgattggct attaattgct tttattggcg cgatgctagt aagcagttta
    15541 atgaatttaa aatatggata tagcgaaaat atcaagttaa tcatgtggca attaatcttt
    15601 ttctttatga tttatcagtt tggcaaagac tacagtagta actttttttt caaagtattt
    15661 acttggatac tttcattggt atggttttta gctaatttag tatcattata ctggtttgtc
    15721 attcaatatg gcataaaaat tccaataaaa tataagtatt acccagttcg tttagggtgg
    15781 ttaggcaacc gtttatttgg tgtatataca gatccaaatt ttggtgcggt tatatcatta
    15841 attgtcatca ttattgctat atattatttg gtcacttaca agcatttaaa tcttctattt
    15901 aaaatatttt ttgcaattaa tatttttatt caattctcgt ttattgcttt atcgggctca
    15961 cgcaccacat tagtttgttt attaatagca acttttgttt ttagtttctt tacaagttat
    16021 agtagtcgtt ttcttaaaac taatacttta aaaagatggc taactgcatt agtagtagga
    16081 ttgctaagta taattgcgat gtattatgta acagatataa ctgctcaagc atgggcacat
    16141 gttccgcata ttacagaaat aaaaaaagat atatctgatt catcaggaac aactagcagc
    16201 aataaagttg atttaaaacg ccctgacact gatggcgggg atgtttctaa tttaaggttt
    16261 ggactttgga aaagtgcaat tgaaatattc aagagtaagc cgatatccgg cggttctcca
    16321 ggatactata ttgaatacgc acatgataaa ctaccatata cattaatggg aaaagatact
    16381 ttaactgctc ataattttat tttcttagtt atggctgcta caggaggatt aggtctattg
    16441 acattgttaa ccttcttatt tatccaatgc tttaaaactt tgcgttattg tttcacacaa
    16501 ccaaatctag taaaagataa gcactttttt gctattttaa tgattcttac aatagcagtt
    16561 tcggcattat ttatcacaga actcattttg gttagtacga taggttcatt tattttttgg
    16621 ggatatctgg gttctatcca aaaaactata tctaaagaag aaggagtaac taattgaaac
    16681 tatttttagg tatataaagt agaaaagaaa actagttatc ccaacttcaa caaattttat
    16741 agtttttaca atataaaaat ggtaaactaa ctttattaag tatgagaatg cataattgga
    16801 gtgaatttga tggatactat attgattact ggtggagctg gttttatcgg ttcaacgcta
    16861 gcgaattatt tgggaaaaga aaacaaaatc gtagtagtcg acgatttatc aatgggaaaa
    16921 aaagaaaact tggatatgga gaaacaagtg actttcattg agggggatgt atcagatcct
    16981 caattgatgg aaagaatcat gaaacagtat cagtttgcct atatttttca tttagctgct
    17041 gtagctagtg tggcagattc tgtagaacgt cctctagaaa ctcatcgtgt aaattttgac
    17101 agtgcgcttt tattgcttga attggtacga aaatatcaat ctggtttaaa acgattggta
    17161 tttagttcat cagctgcagt ttatggcgat gaaccaacgt tgccaaaaaa agaagaatcg
    17221 gtcatccgtc ctttaacacc atatgccatc gataagtttg catcagaaca atatgttttg
    17281 gattactgtc atttatatga tgttccgact agtgctgtcc gattttttaa cgtttatggt
    17341 ccaaatcaaa atcctaactc tccatatagc ggagttattt cgattctagt ggaccgatac
    17401 aaaaaacaat tggcaggaga aaaaacagaa tttactttat ttggtgatgg tagccaatct
    17461 agagacttcg tttatataga agatgttata caagcacttt tacttgttgc taaggaagaa
    17521 aaagctttag gacagcaatt caatgtcggt acaggaaaaa gcactacgct acttgaattg
    17581 attcattcaa ttgatcaaat cttaggaaca gagctagcgc ttaaatatga agcggagcgt
    17641 tcaggggata ttcgtgattc tttagcggat atttcaaaga tccgttcttt aggttatcaa
    17701 cccaaatttg atatattgaa cggaatggaa cgttatttaa agacagaaat cgattgaaac
    17761 aaaaaaccag catcctgctg gtttttttgt ctcgacttag ctttttaaat taacaccttc
    17821 catcagttcg taaatctgat ttcttaaatc agtggccggc aatttgatgt ttttatcaga
    17881 ttggatatta ctttccatga cgattgcatc ttttgtgtct tttttcatcg ccgctactgc
    17941 atggaatcga ttgatatttc cctgagcaag gatataatca tttccactgt aaacaccgcc
    18001 gctgtaagga tgtttatcac cattattttg ccacaatgag aaaagtagtt cttttggtat
    18061 aatatcgcca gacagaacac tggtaaagaa tttgttcaag tcataaacag acatacttat
    18121 atttcctgtg cctaattcat ttcggatgtc tgtttcaggc tcgaccaatg cttttgaata
    18181 atcgtcagtt gcagacattt ggtaagatac agcatgctga ggggaatttt ttacattttc
    18241 ataaaaatct gtatgttcta gatggagtgg ccgaatgatt tcttttttta aaagagtttc
    18301 ataaggctga ttcattagtt tgatcaatac ccctgtaagc aatgtataat ttaatggttc
    18361 ataatgatac gtatcataac cagtaaattt caaatgatga agggcaaact gaatgatctc
    18421 actatctgac ataggttttt ttggagaagc ggttcgatga agaccagagc gcatgtaaag
    18481 catatttttg attgagattt gctgacttcc aggaatctga ggataaaatt cagatagctt
    18541 tgtgtcaaaa gttatcttgc cagattgaat ttgttttagg ataagtaaag cagtcatcgc
    18601 tttttggatc gaaccgattt gatagtaaag agaaggagaa ttcgattctt ttatgcgtag
    18661 atttgcgtac ccatatcctt tttctaatat cactcgatta tttttgcgta cataaatcga
    18721 accgatataa tctgattttt gcagtaattg atcaattttg ctggcaagca tctgctcatt
    18781 agatgttaaa ttggaagcaa gttgtgcggg ttccgtttta taaatttgtg gttgaggttg
    18841 ttttgttaat tgccatcctg taatgatgcc tgttgaaaag aagctgacag ccagcaacaa
    18901 aactgcgaga aaagaaagag agatctgctg ttttttttga tgttttcctt ttgccatatt
    18961 aaatcctttc tatccatttt taactatttt tcattgtaat cctcaaaaag agcatttgcc
    19021 acttacaatt ttgacataat atttggtaaa ataattcacg gcagtagaaa tttcatgtat
    19081 aataaacaga gttacgaaga tgatagtaag gaggagttgt tttgtcagga aacaaacaac
    19141 acccaagcaa agacaaaaaa gaagccagta aggcagctac agcagatata atcgacaagc
    19201 ggagaaatct gcgtaaaaaa gaagataaga tcgtgaggaa gatcatttta gtgatcgctc
    19261 ttacattatt gattattggc ggatttctcg ggtttaccgt atatcgttat gtagatagcg
    19321 ggttgaagcc tttagataaa tcggacgacc aattagtcca agtggaaatc ccaaacggct
    19381 cttctaataa gcagattggc gaaattttgg agaaagacaa tattattaaa agtgggattg
    19441 ttttcaatta ttatacaaaa tttaaaaact taactggctt tcaagctggt tattatcagt
    19501 tagcgcctaa tatgactctt gatgagattg gcaagcagtt gcaagaaggc ggcacatctg
    19561 agccaacaaa agttgcagat gggaaaattg ccattccaga aggctacgac atcgatcaga
    19621 ttgctgagcg ggtagcgaag gtaacaggta aagacaagaa agaattttta gatttagtaa
    19681 atgatgagac tttctttaac gagttgtatc aaaaattccc agaattgttg gaaagtgcct
    19741 ctaaagctga gaacacaaaa taccgtttgg aaggctactt attcccagct acttatgatt
    19801 actacacgag tacttcgctt aaggatcttg tgattgagat ggtgaacaag acaaatacgg
    19861 tcatgcaaaa ctattattca gctatcaaac agaaaaattt gactgtacaa gaagttttaa
    19921 ctttggcttc tctagttgaa aaagaaggcg taaaagaaaa tgatcgtaaa aatattgcac
    19981 aagtcttctt taaccgaatc aaggcaaata tgccacttca gtcagatatc tctgttttat
    20041 atgcattagg agaacataaa gaattggtga cgtatgaaga tacagctgta gattcaccat
    20101 acaatttgta taccaatacc ggctatgggc ctggtccttt tgacaatcca agcgaaaagg
    20161 caatcaaggc agtattagaa ccagcagaaa atgactatta ttatttcgta gcagatacga
    20221 gcactggaaa tgtttatttc gctaaaacgt atgaagaaca tatggaacta gtgcaaaaat
    20281 atgtaaataa ttcttagaaa aacaggggct agtcattgat catttttaga aaaacattta
    20341 cattttaaga aaagcgtggt aatctttttt ggactatatt cctcaaaaga ttaccttttg
    20401 cggtatttta tcgaaaaaag gagacgttac acatggtaga gaaagtttat ccaatgactc
    20461 tagaaggtaa agaaaaatta gaaaaagaat tagaagaact aaaaacagtt aaacgcggag
    20521 aaatcattga acgaatcaaa atcgctcgcg gtttcggaga tctttccgaa aactccgaat
    20581 acgaatcagc taaagatgaa caagcatttg ttgaaggaag aatcacaaca attgaaaata
    20641 tgatccgttt tgcacaaatc atcgataatg acggcgtaga tgcagatgag gtatcgatcg
    20701 gtaaaacagt tacgttcatc gagttgcctg atggagatga agaagagtac acgattgtcg
    20761 gaagtgctga agcagacccg ttttctggaa agatttccaa tgattctcct atcgcgcaag
    20821 cattgattgg caaacgtaaa aatgatgaag taaccattgc tacaccaggt ggcgatatgt
    20881 tagttaaaat cgtcaaagta gaacaagcgt agtctgactg aaagatctag agagttcatc
    20941 ttactgatga cgcactctag atcttttctc tttgagtatt gcaggtttca ctgaacgatc
    21001 ttacatctaa agtcctatag ccaacagtca aaaaaatagg tatgatgaaa tgagaatgtg
    21061 tcaggaggga gaaaaagcga tgaatagttc ttggcgtttt tttgtggtag tcgaagcact
    21121 gctgctgtta tttgctgttt ggcaaatcgt aaacaatact ggacttttgc ttttagtaat
    21181 attcggtatt ttcaatatat atctagctat gcgcaaatat cctcgtacta aatttcaaaa
    21241 ttttcaattg atcttaggga gcttggtcat tttttttagc ttagtcaaca gtcctgcttt
    21301 atggatgatg gttgtattgg ccgttttatt tatcgggctg aaaggtgtag agatttcagg
    21361 aatagatttg acaaaaaatg ctttttggcg aaaaaaacag atcatgatgg tccaaacaga
    21421 gcaattaaaa acacataata atgagcgtaa aagacagcaa ttatttggta accaacgtat
    21481 tggaaacgac gtctatgaat gggatgatat caacattgcc atcatttctg gtgatactat
    21541 catcgatttg ggtaatacac ttttgccaaa agatgataat attgtgattg taagaaaagg
    21601 gataggcaga acacggatct tagtacccct gggagtggct attcgtttag agcatgcgac
    21661 attagtagga aatgtcttgt ttgaagaaga gcagttttcc ttaaaaaacg aacagatcaa
    21721 aatctgcagc agtgattatg atgaaaatcc tcgcagattg aagatcatta ccaatacctt
    21781 ggttggagat gtcgaggtga tacgaatatg atgggaagaa tatccaaagc aatgctagct
    21841 gtttattcgg ggattgcggc tttccttatt attctatttt cacttttcac ttatttttat
    21901 gccagtaacc aaagtcattg gtggggagaa ttgctgcgtg cgcgtttatt atacgttccg
    21961 cttatcttcc atttgatggc aatttcatta ggtgtaggat tgatcgtatt tctattgtta
    22021 tcacttattc aaaaggcaaa atacgggaaa atcgaagaaa agcttcgtgc actttcttct
    22081 ggcaattatg aatccaaact tttgcatcag ccgatcccaa gcgcgtcgga tgatctgtac
    22141 atcaaagata ttgatatgga aattgctaaa ataaaagaaa aaatgatcga agtatccagt
    22201 gaattacaga ttatgactag ccgtcctcaa tatgtagatg gacaaaccaa agaagagatt
    22261 ttagaattag aaaggcatcg attagctcgt gagcttcatg attcagtttc acagcaatta
    22321 tttgcagcga tgatgatgat gtcggcattg acggagcaag cagaaaaaag tgagacacca
    22381 gaaatgttcc gtaagcagtt gaaaatggtg gcagagatca tcaacgcttc ccagtctgag
    22441 atgcgtgcgc tgcttcttca tctgcgtcca gtcaatttag aagaaaaaag tttaaaacaa
    22501 ggcattgagc agttattgaa ggaattgcag aataaaatcc agatttcgct aaaatgggat
    22561 gtagaagatg taaaattaac tagttccatt gaggatcact tgttccgaat cgttcaagaa
    22621 ttgttatcaa acacattaag acatgctaag gctaatgaat tagaagtcta tttgcataaa
    22681 atagataaca atcttttatt acgtatcatt gacgacggga caggatttaa tatgaatgaa
    22741 accaaaacgg gaagttatgg attgaacaat atcaaagaac gagtagctgg tattggtgga
    22801 acagtaaaaa tcattagttt taaagggcaa ggtacgagcg tggagatcaa ggtccctttg
    22861 atgaaggagg catagataaa gatgataaaa gttttattag tagatgacca tgaaatggta
    22921 cgcttaggcg tctcttctta tttatctatc caagaagaca ttgaagtgat tggagaagct
    22981 gaaaatggac gtcaaggcta tgaaaaggcg atggcacttc gaccagatgt cattttaatg
    23041 gatctagtca tggaagaaat ggatggcatc gaatcaacaa aagcaatctt aaaagattgt
    23101 ccaaaagcca aaatcattat cgtcacaagt tttattgatg atgaaaaagt ttatcctgca
    23161 attgaagcag gagcagcagg atatttgttg aagacgtcta ccgcacatga aatagctgat
    23221 gctataaggg ctacgcaacg cggcgagcga gtattggaac ctgaagtgac aacaaaaatg
    23281 atggaaaaaa tgagtcggcg aaatgatcca gtattgcatg aagaattaac gaatcgagaa
    23341 aatgaaattt tgatgctgat ttctgaaggg aaaagtaatc aggaaatcgc ggatgaattg
    23401 tttatcacac tgaaaacagt taaaacgcat gtgtcgaata tattagctaa gttagaggta
    23461 gaagaccgaa ctcaagcagc tatctatgcg tttaaacatg gtttggtgaa ataaaaacga
    23521 tgataaagag gataatcaaa agctgagaaa ataactatta tttttcttgg cttttttaca
    23581 ataatgatgc ttgcttttgt ttttggaaag gctttgttat actgagaaaa gaagatacta
    23641 aaggaggaaa atggtgaaac aaaattttgc aattgtaggc cttggacgat ttggcggaag
    23701 catctgccgt acactgatcg aagctggaca agaagtactg gctattgaca gtaatgaaga
    23761 tcgagtcaat gaatatatga atatagccac tcatgctgtt gtggcaaatg cacaggatga
    23821 gatgacactt cgttcattag gtataagaaa tttcgatcat gtaattgtcg ctattggaga
    23881 agatatccaa gccagtatct tagttactct gatggtaaag gaaatgggcg taccgaatat
    23941 tttagcaaaa gcccaaaatg agtaccatgc ccgtgtacta gagaagatcg gagcagatcg
    24001 tgtcgtacac cctgagcgag acatggggat acggattgca cataacttag tatcaaagaa
    24061 tattctagac tacttagagc tgtcagataa atattcttta gcagaaatcc gagtctccaa
    24121 tcctaaattt ttcaacaaaa cattagctga attaaatttc aggcaacgtt ttgacttaac
    24181 agtagtcgcc atacgaagag tagatcaaac ggttatcgct tctcctgcag cagatgaata
    24241 tgttcgcgaa aatgataatc tactggtgat tggagagaca ggcgatgtgg atattctaga
    24301 cgataagatg aatcaataga aagaaaagag aaagtaggtt gttgggatgg aattaacggt
    24361 aacgccaaaa gcacaaaagt ggtttgactc agaaattgat ctgccagaaa cttacggaat
    24421 acgttttttt ggaaaagtct acggcaaaac agaggttcat gatggttttt cgattggaat
    24481 gtcagttgaa cagccagaac atcctgtaaa gaaagtggaa atggatggga cacttttttt
    24541 tatcgaggaa gcagatgaat ggttctttaa aggatatgat ttacttgttg attatgatcc
    24601 caacctggag gagccaacct atcactttaa aaaacaataa aaaaagagag ggtgacacaa
    24661 gttttg
//
LOCUS       DAFBQH010000033        24189 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.033, whole genome shotgun sequence.
ACCESSION   DAFBQH010000033 DAFBQH010000000
VERSION     DAFBQH010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.033"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(74..619)
                     /locus_tag="LK694_001500"
     CDS             complement(74..619)
                     /locus_tag="LK694_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK5052370.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(631..1323)
                     /locus_tag="LK694_001501"
     CDS             complement(631..1323)
                     /locus_tag="LK694_001501"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBK5052371.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1342..1626)
                     /locus_tag="LK694_001502"
     CDS             complement(1342..1626)
                     /locus_tag="LK694_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052372.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1639..2202)
                     /locus_tag="LK694_001503"
     CDS             complement(1639..2202)
                     /locus_tag="LK694_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBK5052373.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2430..3092
                     /locus_tag="LK694_001504"
     CDS             2430..3092
                     /locus_tag="LK694_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBK5052374.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3216..4412
                     /locus_tag="LK694_001505"
     CDS             3216..4412
                     /locus_tag="LK694_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBK5052375.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4513..5889)
                     /locus_tag="LK694_001506"
     CDS             complement(4513..5889)
                     /locus_tag="LK694_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5052376.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5926..6792)
                     /locus_tag="LK694_001507"
     CDS             complement(5926..6792)
                     /locus_tag="LK694_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HBK5052377.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6881..7906)
                     /locus_tag="LK694_001508"
     CDS             complement(6881..7906)
                     /locus_tag="LK694_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK5052378.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7924..8598)
                     /locus_tag="LK694_001509"
     CDS             complement(7924..8598)
                     /locus_tag="LK694_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5052379.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8782..9675)
                     /locus_tag="LK694_001510"
     CDS             complement(8782..9675)
                     /locus_tag="LK694_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK5052380.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9749..11446)
                     /locus_tag="LK694_001511"
     CDS             complement(9749..11446)
                     /locus_tag="LK694_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBK5052381.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11439..12752)
                     /locus_tag="LK694_001512"
     CDS             complement(11439..12752)
                     /locus_tag="LK694_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBK5052382.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12752..16270)
                     /locus_tag="LK694_001513"
     CDS             complement(12752..16270)
                     /locus_tag="LK694_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBK5052383.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAISDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16563..17021)
                     /locus_tag="LK694_001514"
     CDS             complement(16563..17021)
                     /locus_tag="LK694_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK5052384.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17319..17837)
                     /locus_tag="LK694_001515"
     CDS             complement(17319..17837)
                     /locus_tag="LK694_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBK5052385.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(17839..18456)
                     /locus_tag="LK694_001516"
     CDS             complement(17839..18456)
                     /locus_tag="LK694_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBK5052386.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18453..19307)
                     /locus_tag="LK694_001517"
     CDS             complement(18453..19307)
                     /locus_tag="LK694_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5052387.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIILLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19304..20215)
                     /locus_tag="LK694_001518"
     CDS             complement(19304..20215)
                     /locus_tag="LK694_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5052388.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20256..21590)
                     /locus_tag="LK694_001519"
     CDS             complement(20256..21590)
                     /locus_tag="LK694_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK5052389.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            21798..22628
                     /locus_tag="LK694_001520"
     CDS             21798..22628
                     /locus_tag="LK694_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBK5052390.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(22872..23190)
                     /locus_tag="LK694_001521"
                     /pseudo
     CDS             complement(22872..23190)
                     /locus_tag="LK694_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23312..23506)
                     /locus_tag="LK694_001522"
     CDS             complement(23312..23506)
                     /locus_tag="LK694_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052391.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT         7146 a         5253 c         4166 g         7624 t
ORIGIN      
        1 aaacaaagtc gtttgtttta tgctcttctt ttattatttt gtgcttttta ttttatcaaa
       61 tcactttgtt tttttataaa atttctgcgc ctagtgtgga ctcaaaatgt ctcaatgccc
      121 attcatgtcc aacgggatcg aaacttgcca cggcatcttt gaaaatatgg agtttgtatc
      181 ctagattata cgcatcgact gctgtatgca acacacagat atctgtacat acgccggtca
      241 aataaatatc tgtgatctgg cgttctcgca aacggatgtc tagatctgtt ccactaaaag
      301 cggaataatg ccgtttgtct atccaataaa cattttcctc ttgctcatag tcttggtata
      361 aactttttaa agagccgtac aattcccgtc cacccgttcc tatcacatta tgcggaggaa
      421 ataagcggtt ctccggatga aaggaatctt ttggatcatg cgcatcaatg gcaaaaacga
      481 caaaatcctt ttgatcaata aacttctttg tatgagcagt cagctttttt tcaattactt
      541 gtcctgctgt cccagtagtc agctttccat cctctgcaac aaaatcatat gtgtagtcaa
      601 tcgagatcaa tgcgttcatc ttagtcctcc ttatacgaga tgtttgacaa attcgatcac
      661 catttctgct gatcgtttgc ctgcgtcttc gataaattcg tcaaagcttt gtgtcgccga
      721 gtgatctgct gtgtcgctca ttgcacgtac gatcaaaaat gggatattga actgacgagc
      781 tgtttgtcca acagcggctc cttccatttc gcaagctaat gcttcaggga aattcgttaa
      841 gatttctttg attttatctg gcgagtcgac aaatgtatca cctgtaacga tcaatccttc
      901 ttttgcgttc aaattcgttg cttttgctgc tttaaccatt tcactgcgca aatatgtgct
      961 tgcttcataa tatagaggca ttcctggtaa ttgtcctggg ttgtagccaa atcctgttgc
     1021 atctgcatca aaataagcta ctttgtctga tataacgatg tcacctactt gtaagccttc
     1081 accgattccg ccagctgaac ctgtattgat caccatgttc acgccgtatt gttggatcaa
     1141 cagacttgtg gtaatagatg ctaagacttt tcctataccg gaacgtacga cgatcacttc
     1201 gtggttacct aatgaacctg aaataaataa agcgcctgcg cgttcccatg ataatggttc
     1261 gcttaggttc tctctcaaaa tttttacttc ttcttccatt gcgccgataa taccgatttt
     1321 catcttttta attccttctt tctatataaa tgcgattgct aaaaaaacca cacataacat
     1381 taggataaca ataataagag atttcatcaa aaaagagttc catttggtcg tcttttcccg
     1441 ttcgctgatc cttgttttcg tgattggttt attctttttg ctttctttac gataaaagct
     1501 ggcaatcttt ttttcttctt gctgataagc tgtttctgct tttcgctgtt tttttaatga
     1561 ctcgcttgct tgtgcttgtt gtcgcttgcg aagttcgctt cgcgtaataa gcggtccttt
     1621 ggccataagc cgatccctct actttccttt ggtcaatagt tcccagtatt gaatcgcata
     1681 aatcgtcttt gcatcacaaa tcaactgatc tttcatcgct tgttgcgcct cttcaagagt
     1741 tagatgatac aactccagta cttcgtcttc atcttgtgct aaaggattct cgaccttctc
     1801 taccccttgt gcatgataaa tatgaaggac ttcatttgca aaacctggtg aaagatacat
     1861 cgaagtaaga tggctcagcg acttcgcacg atagccagtt tcttcttcta attctctagc
     1921 tgctgtcatt tcgggatttt gtccttctcc tggatcgatt tttccagctg gaatctccaa
     1981 aatcactttt tctaaaggtt ttcgaaattg tttgacaaga agcagacgat cttgttcgtc
     2041 aaaagcaatg atcccgacac cgccaggatg aaataccagt tctctcttcg aagttcctcc
     2101 atctggcaaa cgaacttcgt ctacagcaac atcaataatt tttccgctat agatttcttt
     2161 gcgactgatt gttttttctt caaattgctc attgttcagc atatcttctc ctgctttctt
     2221 tgtaatcata actgcttttt tttcattttt caatttttct caaaaactcc ttattagttt
     2281 accatagata aaccttagat tttctctaaa tatagaaaat ccttctttag tccgatactc
     2341 tttatagtag tccgtatgct aaaataagta tgtaatcgaa taacgaaaag aattcaatac
     2401 tcaacgataa atgacatgga gggtttatta tgataaaacg aaattggccg tggcttctag
     2461 cattggtctt gctatttctc tttgtccgaa acaatagttt ttcttttgtt ctaggacttt
     2521 tattgatagg tgtagtttta gttttgtggg gactctttgg ttcgcgcagg aaaaagggaa
     2581 aacaagaaga aattcctgca ctgactgacg aacttgaaac tcactatgaa aaaagcggta
     2641 tgacagcgaa tgaaatttca ttttttcgac aaacaatgaa tcaaacaaaa aatgagatca
     2701 atcaattgca aatgaatatg cagcagacag ccaaacttaa agcaatcgat ctccgccatg
     2761 atccagtcaa agcagcgaaa gctttattca aagaattggt gaaagaacca aaccgactgc
     2821 atgaagcaag tcagtttctt tatacgcatc tgccgaatct agttgatttg acaaataagt
     2881 ataatgagat caatgaccat gagatcaaaa acaaacagac ctatgaaaaa ctagaagaaa
     2941 gcgcgcagat catcgaccag ctgtcagctt taatcgcaca agattatcaa aaattcgttg
     3001 ctgaggatct agatgatatc gacgtagaga tatctgttgc gaaacagagc ttaaagcgtg
     3061 ataataaata tgaaccagaa caaaaggatt aactcttttg ttcataaaag aagactcgga
     3121 acattcagat gatgtctggc tgtttcgagt actttttcta aagtagagac attcatgtaa
     3181 gaatcgagaa cagattcaac aaggaggaat tttttatgga tcataaaccg actgaaacaa
     3241 aagatgttac accagttaac gatacacttg aagatttatt gaacaacccg ttttcaactc
     3301 caacagatac gctgaccact tcacaacaag cggagattga tcagctccaa gagcaacaga
     3361 cagctgctcg tttaattgac aaactaccgg cggaacgcca agaacaagct aaacagctag
     3421 cagcaaaaat cgatgctaat gatgcacagt ctgtcatcag ctacggatcg gctgcccaag
     3481 caaaattaag tgaattctca caatcgatgt tgaatcatgt gcaagcacaa gatattggtc
     3541 ctgtaggaga ttccttaact gaactgatgt atcgtctcca agaagccaat cctgacgaac
     3601 tgcgtgccgg agaagggaac atcttccagc gtgtcttcgg gaaggtcaaa caatcgatct
     3661 atgaagtgac agcaaaatat caaaagattg gtgcacaaat tgacaagatt tcagtgaaat
     3721 tagataaaga aaaagacggt ctgctaaaag ataatctgat gttagaacag ctttaccaga
     3781 aaaacaaaga ctactttgat gcactgaata tttatatcgc tgctggagaa ttgaaaatgg
     3841 aagaacttca aacaacgatc attccagaag cgatgaaaag agcagaagaa actggcgatc
     3901 agatggatgt tcagattgcc aatgattaca cgcaattcct agatcgtctg gataaacgta
     3961 cgcatgatct tcgcttagct agacagatca cgatccagca agcaccacaa atccgtttga
     4021 tccaaaacac aaatcaggcc ttggctgaaa agatccaagc atctatcgct acagccattc
     4081 cgctgtggaa aaaccaagtc gtgatcgcat tgaccttgct tcgtcaaaaa gatgcagtaa
     4141 ctgctcaacg ccaagtctct gaaacaacaa acgacttatt gaagaaaaat tcagaaatgc
     4201 tgaaaatctc agccatcgaa acagcgaaag aaaatgaacg tggaatcgtg gatatcgaga
     4261 cccttcaaac cactcaaaat gatttgatcg aaacaatcca agagacattg cgtatccaaa
     4321 aagaaggaaa agaaaaacgt cgtcatgcag aagtagagct tggacacatg gaagaagatc
     4381 tgaaacaaaa actgttggat ttaacccaat agatcacctt tcgcttgtta ctaaaacaaa
     4441 cgcccaacaa cagtttatta caactgttga tgggcgtttt tcttatcttt cttctaactt
     4501 ctccattgga gtctatttct cattcatctt gctgtgtcca tagccatagc caaaataaat
     4561 gatcagtcca ataaccatta cgataccgaa cacgatccat gtcacagttt gcagacgaag
     4621 catcaataca atacaagtca agatggagac gattggcaat agcggaacga atggcacttt
     4681 gaattctcca gcttttggtt tgccaaacat ttttctcaac ttcagtaagc ctagactaac
     4741 aagcatcaaa taggcgagcg ccatgatatt cgttagttcc gctaatacat tcaatgggaa
     4801 aaatccggca aagaatacag ctgccacacc agcggcataa gtcgcttttt tcggtgtacg
     4861 atgcttttga tcgatttctg tcataaactt aggaagtaag ccatccttgc tgacagcaaa
     4921 cagtaaacga gcaagtgaat acatcatcga gatcgttacc gtcagtagcg ttaaaattgc
     4981 ccctacagcg atcaccgcgc ccactgttcc atgtcctaca tacctcattg caaaagccac
     5041 tgggtcattg actcctagtt cagtatacgg aacgattcct gtcaagatca gcgtcacgat
     5101 gatgtaaagg acagtagcaa tcagtatcga accaatgatt cctcttggca catctttttg
     5161 cgggtttttg acttcctcgg ctgccattgc aacggcatca aatcccaaga aagcaaaaaa
     5221 cacaagagct gcaccatcta gcacaccgga aaaaccaaaa ggcatgaatg gctgccagtt
     5281 atcaggttta acgaaaaata ttcctacaat gataaacaac gcgatgatac caaatttgac
     5341 atataccatg gcattattca aacgtaatgc tttcttggct tcaagcgaca cgatgtaagt
     5401 gacgaatatc actacgagca ccgcaatcaa gtcaatgtat gtaccatttt ccggattgta
     5461 cccgccagtt aatgcttgtg gaagttcggc ccctaaactt ttcaaaaaac cttgcatata
     5521 gccagaccat ccggaagcaa cagaggaaac cgctagtaaa aattccccga tcaacaacca
     5581 ccctgtcagc caggcagcaa attcaccaaa tacaacatac agataggcat atggcccacc
     5641 aatcactggc actcgcgaag caaattctgc gaaacttagt ccagacaaaa tgacaacgat
     5701 tgcagctaag acaaatgaaa gtgacagtgc tggaccagca ttttgattgg ccgcaacccc
     5761 tgtcaccaca aatattcccg taccgatgat cgcaccaatt ccaagcatga tcaaatcaga
     5821 cgtttttagt tcttttttca tctcactcgt atgattgaga ttgatctctt tcttacgaaa
     5881 caaatccatt gaagttcctc cccattttag gttggaaaac agcgattaaa ctgttttcat
     5941 ataccctaaa aggataccac ctaaaatgac taaaaggcaa ccaacaataa cataaatgag
     6001 ctcttttttc gtttttcttt cgccaaggat gaaaatccca cctaaagtag agataatgat
     6061 ccccatttgt gaaagtgaga agccaacagc taaaccaagt gatttgactg ttagaagcat
     6121 gcagatgttt cctaagcccc ataataatcc acagatcatg tttttccaca caaactgatc
     6181 gactttaact ttacggaaag caaagaagct tgcgcctaag atcattccga tactttgagg
     6241 taagataatt gacatcgcat ccaaattcgc ccagttgacg atgatggtat atacaccata
     6301 accaatcgta gagaagatca atgctctaaa acccttacca aaatcaagca ttttattatc
     6361 tgtatctatt tttccttcgt catcttgtcg ggcagtcaaa tacgcaccgc ttacaagaag
     6421 agctaaagca ataataccgt atacataatc tttcgtttgt gtccattcat ggaagaaaac
     6481 ggctcccgca atcgtattca aaattaattg aaaaccagta gacaatggta gccctacaga
     6541 aacacccatg tacttcattc catggaactg tccattttgt ccaacactcc aagctaagcc
     6601 ggataaaaat ccgatcaaga aaatccaagg ggtgattaca ggactaacaa cgaaaaagac
     6661 aactaaagaa aacagaaatg caccgatcgt cattcctagt gtttgttgat tggcatcgcc
     6721 accaatttta ccacttacta aaccaatact accccatgcc aacataggga ctaatgctat
     6781 taaaatttcc ataaccaatc tccttttatt tcacatgata agaaacttat cagcgaaaac
     6841 gttaacaaca caagcacttt ttttcgtttc taacgatttt ctaacgtttt tcattataaa
     6901 aatttaagat tgggaaaaat aaagtcattg ttgtcccttt tccttcagtt gattcagccg
     6961 tcaaatggat acctaatttt tcggccagat tttttgctaa ataaagtcct aatcctgttg
     7021 aatgtgtttt gctaagtcga ccatttctcc cagtaaatcc cttatcaaag atccgtttca
     7081 agtcttccgc tgggataccg atccctgaat ctttaactga aaggctgact ccttgcgctt
     7141 ccttttctat gctcacaatg atgtttccgt gatccggcgt atatttgatg gcattactaa
     7201 gcaattggtt gaagataaaa gcgatccatt tagcatcggt caaaacagta tgatcctcac
     7261 caacgatctc gtagcgaata tttttttgga taaaataatt tgcctgactg cgtatcacag
     7321 gctgaatgat tccttttagt ccatattctt ggatgaggta gtcccgcgag aaactgtcga
     7381 gtcttgcata atacaaaact tgttctacgt attcatcgat ttttgataat tcattttcta
     7441 gtaatgtata cttttgatcg tcaatatcga actcgatcga acgaactagc aatttagaag
     7501 cggaaagtgg cactttgatt tcatggaccc atgagtcaat ataatctttt tggtcttgtt
     7561 gattactgat tgctgattgc attacttctt ggtgttcacg gatcagctga ttgatatatt
     7621 cttctaccag tttttcttct tctgtcctcc caccactcaa ataattttgc aaaggggaag
     7681 ttttttgtat gtcggctaat tttccccacc atcttctctt gctcaagtaa ccacccagca
     7741 gataaaaaat cagaaagacg ccctcaatca aagctaaata agcaattgtg ctcaaatcaa
     7801 ctttgatgtt tggcgccagc cacatcacaa agatcgtcaa tgcaataaaa aacagccatc
     7861 ccaagatcaa tgtccactga tctttcaaat atttaaaaaa attcatctta accgctcatc
     7921 ctttcaggga acgatatatc cctgtcctac ttttgtttcg atatatcctt ggatacctgc
     7981 ctgttcgatt tttcggcgta accgattgat gttgactgtc aatgtattgt catctacaaa
     8041 tcgttcgtca tcccacagtg ctcgcagcaa tttttcccga cttaagatct ttccattgtg
     8101 cttcatcaaa ataaataata gacgatattc atttttgctc aaatcaatga tctctccgtt
     8161 gatttccata cttccgttgt caacattcaa cgtaatgcca ttgtggctca tcatttcact
     8221 agataattct gtgtagttgt aagaacggcg aagtaatgca ttgatttttg caatcagtac
     8281 atcgatttgg aaatgttttg tcacaaaatc atctgccccc atgttcatag ccatgatcat
     8341 atccatattc gtattcctgc tggagataaa aatgatcggt actttcgata cttcacgtat
     8401 tttttgacac caataatagc catccaaaac gggaagatta atatctagta atacaagctg
     8461 tggttcttct ctttcaaagt ctgagaacac ttgattgaaa tcggtcgttc caaaggtttc
     8521 aaattgccat ttctcaagtt cctccatgat caattgtcga atgatctctt cgtcttcgac
     8581 taccatgatt tttgccataa aaaatccctc catctttctt cttgctacta caatatcaaa
     8641 gaagcccttg tgaaacaagt tggcttacct ggaaaatgtc attttagcaa acgttaaaaa
     8701 aaactggaaa tatgcgattt aatcgcgttt gtctcttcca agcagcttaa atttattatg
     8761 ttattcttaa taattggtct attatctgtt atttttcttt cgttgttttt gtcttttaaa
     8821 ttttcgataa caccaataac cgaaaagcgt tagcactagc aatataccta gaaggagtat
     8881 aagtaatcga taaagatggt atttttttgt ctgttctatc tttgaagaga cttcttctgt
     8941 aaagggaact cgtcttcctg tcactagtaa tcggtgagtg ttcacggtgt aaggtgtaca
     9001 agtgataagt gtgactagat cctgcttttc tctaattgct aatgtatttg tttttgttgg
     9061 aaggacaaca attttctcaa cgacttcata agcgagcatc cgaccataca cttcaagata
     9121 gaatcggtca tcttgctcca tttcttcaag atccgtaaag agttttttct cagcaatacc
     9181 gctatgggcg gtaatcacgg aatgtgtact ctctcctcca gttggaaaag aggtgccttg
     9241 caggactgtc gctcctctat ctaacaaaag atcattcgtt tcatcaaata aaggcaagct
     9301 aacattgatt ttcggaatat agatggctcc gatcatatgt tgttcgtaat attcttttcc
     9361 tggattttgg acatttctaa ccgcttgctc aaaaggatct ttgacttgtc ccattcctgg
     9421 gattactgat gtatgagcaa gcgtctgatt tttcttttca tgcactgcta attgttgctc
     9481 atgtttttct tctttatcgc gttcaagctg cttttgataa ttctcgatcc ttctttgatc
     9541 caaataattg ctgagagcat ctgctaaaaa tggatatgta aaaactgcag ctccggcaag
     9601 aaacatcact gccataaata attgtaataa aagactgatt tgggtttgag gtctattacc
     9661 tattttcttt ttcattttct tcaccttctt atttaaaaaa actgcagcaa cctttataaa
     9721 accgattgct gcagcttatt tttgttgctt atacttcaga cttcatttta gatttacggt
     9781 accagctgta cgctccaatc atcaaactta ttccgattag taagaatgcc aagagcccgt
     9841 tcccaccagt agcaggaagt gacccgcgtt tcatattcgg tactctttct ggccctggat
     9901 tttctactcc tacgatctga atttcttcat tatagcttcc ataagagatg ataaattcag
     9961 taaaggctgc ctcacctgtt ggaagtacat aaccttctgg cgccatggtc tctttcattt
    10021 gataggttcc ataatctaac ccttgaacct caaagtatcc atttccatct gcgacaaatt
    10081 ctgttgcttg tccgcttgtt gcccactcaa ttctatctgc tcctgattca taaacagcta
    10141 aaggcgactc ccctttataa aatattgcgt attcagaacg gtcttcattc cacaattcga
    10201 atcttgcatc tttaagcaat tcactgcttg atgcatcata tttttcaaac tttcttccac
    10261 ctgttgtgac tttctctggc ggctctactc cttcttcata atcaaattca tcatttcgtc
    10321 cgatttcaat aaccgctgtg ttgttatgga aatcatccgc ttctagttct gtggaaacct
    10381 tcaaattata cgtgatcgtt aaatctcctc cacgtgcatc tgctaatcta cctaaagtat
    10441 ctactttggc tactccattt tcttctgtca aaatcgtcac gacaaacccg ttgcttacta
    10501 cttctactat ataatcttgt ccttttgtta aaatcgtctc gccggctcgc acttctattt
    10561 cctgatcata ataagttaga ccaactgtag gcgtatctgt gattttgtaa tttttgataa
    10621 cagcgcttcc gttttcaagg acagaatcga tgaaataagg aataggtgct gtgatcgtgt
    10681 aagaagccgt ttttcctctt tctagatcaa tataagaaat cgtttcttgt tctccagctt
    10741 cattaataat cgttacttgt ctaaggtcgc tttcctctac attcatctgt ttatctgctt
    10801 ctctgatttc gtttttcgga tacaaatgca catctgtatt gtatgtgtct ttccatgtag
    10861 aaccatccca cacgccatcc accatatcag gcatcatcac tggcatcata acaaccattg
    10921 gagacgcgat tctggaaata tgagctggcg aataagtttc taagaaaaga tagactttat
    10981 ctctagaaga aggtgcttct tccgtcactt ggacggtaga gaataccgct tctccagctt
    11041 cattcgttgt ttgtttgtca atacttgtca gtcgatattt taaaaaccaa ctttctgtgt
    11101 gccgtttttg aatccattca atcgcttcgg cagaattaag accgtcttct cgttccggtg
    11161 ttaaaggatt atctcgaatc aaatcgtaat atacttccgt tacgtcaaac aaatcaaaat
    11221 cgactcctgg aatattttct tcaccaaaat ccgtagaaac caatccgcta ttcggtcgtt
    11281 cttccggaac agatgagaaa ccttttttgt gtaatgtaat cgtcacttca cttggttttt
    11341 gtgcgacttc ttcggctttg actagttttc cgttcccgaa aatccctatt cccgcagtaa
    11401 cgagtaacag tacactcatt cccgtgacaa tcattttttt attcattttt ttctccccgt
    11461 tcttttttta ttaataaaac agcacctaac aataaaagaa ttcctagtaa agaaatcaac
    11521 gttcttgctt cattcgtttg cgggaatacg gaccattttc ctgattgacc tggattgctt
    11581 ggaccactcg gctctttcgg tttgtccgtt gtttctttta cggtctcgtt ataaacacgt
    11641 ggtgttgcgg attcataggc agagactgga actttcccgg attctaattc agccatctcc
    11701 tgtccgttaa ctactacata ttgtggttgg ccttttgcat ccacccaaaa ttcagggatg
    11761 atgacctcga ttctcttact tgcttcatcc atctcgtaac cttccacacc ttttaattct
    11821 tcaaaataat aggtgccaga aggcaagaaa cgttgtcctg tcgtaacaag gccctcttta
    11881 tccgaaatga atttgcttac atttttgtca ttcaaaggat cattgtctgc tggtttgatc
    11941 cattgatttt tcaagtcatt agcaggagcc atatctaaat aatatttccc cccttctatc
    12001 atctgatata gagcaaaaac agcaccttct aatggttttc ctttttcagt tgttcctctt
    12061 tgacgtccat acttgaagaa gtatggatct cttagataac cgacattttt aggatagata
    12121 tgaatctctt gcagttcctc ggttgattct gtcgggtgat aaattggcaa aatagcagca
    12181 attgggatag ctgtctgatc gatatcaaca tccaatccaa cttctggatc aatcgattct
    12241 tcgaaaatca aataaactgc atcccgatct tggtttttct taggtgcttt gatacgtgca
    12301 atcccgtctc ctaatccgcc aaccgtatct ttcccagttg ttaccgaagt ccctatttgg
    12361 acaagccttt cttctgtgat tagctgacgg atattgtttc tgttcgtttc agcgatttgc
    12421 tggacgaatt ctttaggagt catgttttca gcagcttttt tttcatgata ataatcggtc
    12481 atgtcgtaaa tggcaaatgt cgctccattt agtggcaccg tctgtgaaat gatatctgct
    12541 gtttcccctg cttcctcaga gatagccaag ccatcatttt catggtattc aaattgatcg
    12601 ttataatcga tatctcgaat catccgttta tgcaacacga attccacgac atcttcccct
    12661 gattcttctg ccgacatcgc atgtggaaga aggactgctg caagcggtag taaaacaagt
    12721 aattgaagca tcaaacacaa cagctttttc attaccttgc tccctccttt ttagattgtc
    12781 tgacaaacaa accataggct gctcctgcaa gcgccatgaa gaatgcagca agcaggatat
    12841 agaataatcg tccggatcct cctgtagcag gcaacggatt ttcaggatcg ttcccgacac
    12901 taagctgtaa tctgattcga tttgtctcca aaagttcata ttctaactct tgctgtagat
    12961 caggcgtctc tgcttcttcg tttggataat acgaaatctc aacttttagt gtacccgctt
    13021 cttttcctgg aactacccga tcattttcta tgtcaggttc ttctcgataa ctaatttcta
    13081 aaataaagta acctggaagt tttctatagc tgtcaggtga cttcacttcc ttgatccgat
    13141 agaccccttc caacaagtct gaagcattga atagggaaga atcgtttgga tctggtgcca
    13201 agcctccctc gatgatttca tactctcctg cactattttg tttctcaatt gaaaattctg
    13261 cgccttcaag acgtcggcta ttcatactgt caattttcaa aagttccaaa tcgattggcg
    13321 ctagttcatc ttctaaggtt acttgtagta caccatcgat gatttcatag ccttccggca
    13381 agggagcagt ttcgtcttcg atgaccgtat cttcaatgat aaacttacct tcttcattca
    13441 gtgcaaactg gaacacttga tcagatagcc gatacccatt tggtgcgaca gattccgaga
    13501 cctcatattc accaatagac agatgcagag gttggtcgta atttaaatcg acatactttt
    13561 ctactcctac ggcacgagaa tagaagtatc cgatcccagg caaacgtttc gattcttctg
    13621 gttctaacgg attaccagtg tatgctcgat acagcgggaa ggaagaatcc cccactttcc
    13681 tgatatcgaa acctgcacca tctaatgtat ggccttcatg gtctaatttt ctaactcgga
    13741 tctcccgata atcaaacgta ttgtaagcag tgaatgctac ccataaacga tcctcttcat
    13801 ctgttgtata cgtgagatat tgccaatttt cttcacctgt ttgcttgagt tggatagtcc
    13861 ctatagcatt atcgtaaagt cgcaagatcc atgtgtcatt ttcatcaggt tctttgaaac
    13921 cttctgggtt ctggctctca atgattttgt attctgcata gttggtagtt tcttcactat
    13981 ccttataaac aaatgaatct ggctctccga ttggatgaat aactttctca ccatccacga
    14041 ttacatattt cccgtcttca tctactgaat agaacgtgag tctccctttc tcttcctcta
    14101 cttcgttcac accagaaagc cctttcgcta ctggatatcc gatttccagt ccgttaccat
    14161 ctgtttcata aagttcaaac tctgcgccat tgattggcat attactgcct tcaattagtt
    14221 tattgatatt catgtggatt ggcttgaatt catttcgcat ttccagtttg acatacaact
    14281 catcatactc tgtgccttct atgttaggct cttgtacaac agaaagcttg tgatattctc
    14341 cttcataata ggaattcgga tctttacctt ccggtccgcc gactctcgaa ccagatgtat
    14401 attcatctac aagctcgtag tgacctaaca tctcatcttc gttggctgca tattttttga
    14461 tttggaattg atacgtctca tctactaact gataacgtgg tggtccagcg atctctctga
    14521 tccaaaatat gtcgctgatt gctccagttt catctcctaa aatcggcatc ttcgtttcaa
    14581 agatcgctat tccgtccgtg gtagttttac ttgtttcgat tggttcggct tccccactct
    14641 ctggattttg gtcatataat gcaaattcga ctccagccaa cggctcgcca ttttgaccct
    14701 tcttgctgac ttggaaatta cggcctttat gttcgtaacg aatggaagaa gatgggaaat
    14761 acatccgtcc agtagtgata tcattctcat aatcctccag gttctgaata taggccatat
    14821 tgttcactgt gcaaaaattt ccgtcttgag ctcccgcatc aagttttact tggtacttga
    14881 tcctgaccat ttccccttca cctaaagaaa tcggagaaaa tttataagct tggttttccc
    14941 gtccgacata cgttgtcgga gtcgccacta cttctggtgg ggcttgtcca tgacgttctt
    15001 catcccatat ttctggctct tggtacttca tgccgttttc tcctggatct agatggtaaa
    15061 actgaatatc tgccgatctg ccagctccaa atggtgagac attaaaacca gtttcatcat
    15121 aaagtgtggc ttgctggatc gttctcttga aggtagaaga gatatcctcc aaggctgtaa
    15181 caatgtcatt cttgttgttc gcattgtaat atcgcggtgt tacgccatct tcttctaaat
    15241 cagcaatatt tttcaataca tttcgtccaa tagcatctag tcgttgtctc ccgctaacgt
    15301 ttccatcgat tcctaaacca atggagaaga tacttggcgg gttctcttga tacttgtttt
    15361 tcagccaatg atgataagca actgtgttga tcgcagatga cttttcttgc gaagtaggac
    15421 tgccattcgt attgttatta gaagaaccat caccaaccca gtgagtcgct ttattatctc
    15481 cttcttttac ctctccataa cgatagtgta catttacttc atcttctgga cggttaaagt
    15541 cagatgcata ggtaactgga tgagtaaacc ctgcattaga agtattccca gaaaactcct
    15601 caaaatttct gaacaaaacg tcattatctt cagcaatctt atcttccatc acagtccagt
    15661 tgctccaagc tccattagtg ctcgtacgat aacttgactg aatcggtgca taactgaagg
    15721 tcggtgtccc atctcccaca acgattagga ttttttctgg atttctattt tctccgccat
    15781 tgtctttata caatgtttca tagccatttt taagccctag tgtaagcgga gttcctccga
    15841 ttggtgtacg atttaatgct gtattctcac ttcccgtcaa acgattgata tcatttgtca
    15901 atggaaagat gtccgaactg atttgctcat ggttattgat agctgtacta ttatggtaaa
    15961 aattcaccat accgattcga atatccagac tcgggtcaga gagcagatct ttactcattt
    16021 cgtttactgc ctcaatcaac gcagcatttt tagtttgact attggtccca gcggttaatt
    16081 cactcatact cgctgactta tctagaacaa aaacgatatc gattggactg atttcctttt
    16141 tctcgccccc gatgacatct agatagacat tgtaaagggt tgttgcatct tcaggatgta
    16201 gatcagatgc tgtgctgctt ggcagaacag gttctaccca ttttttgatg tacgccccgt
    16261 tataatccaa gtaactgttt tcaaaattcc gttcatttcc ccaaagctga acggtttgag
    16321 ctggaacgtt tctatcttct tcgcctgcag ttgatccatt attagtagca tagtcgtaat
    16381 ttcgccagtt cgctcccccg tttgtataat cactatcatc gatcaagttc tctccgtcat
    16441 atggcgagcc tttgtctcct tgatagcgtt cattgctttg tgtccaattt cctaacactt
    16501 ttgtatagtc attcgttgta aacttcatct attctttcat caaccaccac ttgaagaggt
    16561 agttaccctt cgttcacatc ggggatcttt tctgtttcaa aaatcaatgt gccggtttct
    16621 gcttcttttg aataaacttt tccgacatac cattcttgac catcgatcgt ctcgactttt
    16681 aggtcttctc catcttcatc ataggaggat agacctgttc cacgaacgtt ttttacggta
    16741 ccaatcccgg cggctgccgc atcgattgct aaacgtattc taccttcatt ggcactttcc
    16801 tttttttcaa aagccagatg ccattcaagg gtttcttctt tttcaatata cttcccttca
    16861 atttttgcat agtcctcatc taagaatacc tcatcctcct ttgtttctgt tgactcagca
    16921 gctacatcca gggtcgtcat atgatagact gactggaaat atggtccaaa tagcaaactg
    16981 acagttacta actgcaaaag cttcaatttt aaccctttca catccttata ctcctttcaa
    17041 aaatatttgt cttctttcac aacaatcaac aaaacaatgc ccatctaatt ataatttaat
    17101 ttaattttaa ttttaaatca aatgatatcc tttttagtta aaaaacacca attatacaac
    17161 atttattatt aaaattaaat tataaaattt tataacgttc tcatttaaca aaacttaagt
    17221 tattaagaca aatgaataaa caactacgct ttcctcgaaa caaaaaaata aagcacaaat
    17281 gcgcatttac gcactcatgc tttattcgta caattaattc agataacggc agcgcatagg
    17341 atgccaataa cacttcctaa aatagctcct cctaaaatgt cagtaggata atgtaaaagt
    17401 aaaaaaatcc tcgaataagc gatcaaacaa gcaaataaaa aggctaatat tcctgcaact
    17461 ggacttaatt cgaacaacgc agctactgat aaaaaggcta gcgctgtatg tcctgaagga
    17521 aaagaagaag tcttcggttt acttgtgtaa aggacaagcc gacgattttt ttcataaggt
    17581 cttttccgtc ttgtcgtttt tttgataaaa agcaaaccaa caaaaaaata gatcaaactg
    17641 ccgataagaa tggctagacc atattctcta gtatcaggta tcaagactaa aacagcagca
    17701 aggagcaacc aaataaaacc accattagag atattactga taaatttcaa aaaattgatt
    17761 tttcgactat ccgttttccg ttgcttggcg aacttgttga taatactatc atcgatttta
    17821 gcaatcaaac gcaacatgtt attcctcctt tttaagaacc ttttcttgcg tacgatggat
    17881 aaaaagcagg acttgaaaat aaaacaagaa actgaaacct aataaagcaa aatagattgg
    17941 tcctgcaaac cagaatgcga caagaaggat caacgtgcct gccaaataga aaagcgcgct
    18001 tttgaaatca ttaaaaaaca agaaaaatgc agtaatccaa gaagcttctt tttgttccag
    18061 ttgaagcaag attttgtaac taaagagaag cgcgcttaat agaagcaaaa acaacgtagt
    18121 atacatcaat gtatagccaa ttcccccacc ccaatagcgg aaaatctgat agtcgatcaa
    18181 tagaagaaga aaataaacac ttgctaaact atcttgcttt ccataaagag agaagttctc
    18241 acgaaacttt ttccagaata gtgaaccgag ctttttctct tcattttttt tgtaatgata
    18301 aatcatatac agaactgttc gacttgcagt tcctaatgtg aaaacaggca agacgcacag
    18361 gaaccatagt agctgtaaat ggataagacg tggaatccat tcaatgattt ctaagaaata
    18421 atcattggct tttttaccaa tcgatcgatt catcatcctt ttgaacctcc tgtcaaatcc
    18481 atcccttcaa tgaaataacg ttgtgcaaaa aagaacagca aaatcgtcgg gatcaagaca
    18541 agtgttgctc ctgccatcag ataattccat tgtgtcgttg cttggttctg aaaaacttgc
    18601 aagccaatct gtaacgtgta tttttcttga tcttggatat ataacagagg acctaagaaa
    18661 tcattccaag ctgcgttgaa ggaattgatc gcaatggtaa tcaaggcagg tttcgtcaat
    18721 ggaagcatca aatgactcca aatatacaaa tgattcgctc cgtcgatttc agcagcttcg
    18781 atcaattcat tattgatcga taagtaaaat tgacgcatca gaaatatatt gaacgcattg
    18841 ccgaagaacg atggaacgat caggggcaaa taagtaccta cccagccgat cttggagaat
    18901 aatacatatt gagggatcat cgttacaaac cccgggatca tcatcgtaga aagaaccaaa
    18961 gcaaacatca gctttttccc aggaaaatca agcttcgcaa atccataagc aataaaagaa
    19021 tttgaaagga cattaccgat cactactagc acagtgatga ataatgtatt tcttgcatag
    19081 cgccaaaaag gaaaggcggc aatcgtatcc agatagtttt cccatacgat tttttctggg
    19141 aaaaaggtcg gaggataacg aacgatttct tccattggtt tcaatgaagt aaacaccatc
    19201 caaagcaata gaatgatcaa aatgattcct attactgtca tgaggacata acttagtatt
    19261 ttttttgttt gccgattcac actggtcttt ttgaaataat ccatcatttg ccgtcacctc
    19321 cagaataata gacccactta ggtgcaagct tcaaattgac aaacgtcaga accaacacga
    19381 tcagaaacag gatcattgac atagccatcg cataacccat atcaaaagct acgaacgcct
    19441 tgttccacat atgcagatta taaaaaagca aagaatttgc aggtccgcct gttccatttt
    19501 tcgtcatgac ataagcttct tgaaaaattt ggaaagaacc gatcgtagag gtgatcacat
    19561 cgtaaaaaat aataggagtg attaatggca aagtaatact tttgaacttt ccccaaacgc
    19621 ctgctccatc gatttctgct gattcatata aagatctagg aacattttgt aatgtagcta
    19681 agtaaagtag cattgctcct ccagaactcc atattttcat caaaatcaac gcaggtttcg
    19741 tccattgtgg atcaaacagc caagctggtc cagggatatg gaaccagccc agaaatgtgt
    19801 tgatcaatcc tgcagatgga gaaagcagct gcatccataa gatgtagact gctactcctg
    19861 ataagactgc cggtaaatag aaagctgtac ggaatacact gattcctttg attttttgat
    19921 tgagtactgt ggctaaaaac acactgaaaa tcgtcgtcag cggtacattg aagatgacga
    19981 aataaacggt gttgtataac gatttccaaa ataaatcatc gtgaacgaac attcttttga
    20041 aatttgctag accaatgaaa ttcatcttgg atgtcaaatt ataatcggtg aaactaccaa
    20101 taactgaaaa aagcataggc cctagcataa ataccaagaa cccgataata aaaggcgaga
    20161 taaataaata ggcccatctt gcttcttttt tctttcggat agataaacgc ttcaaatttt
    20221 cttgcctcct ctgtacctag acagtcaaaa atggattatt gattttgctt gactaagttt
    20281 tctactgctt tttgtgcatc tgccaatcct tcttttggtg attttgttcc taaaagaatc
    20341 tggtcaatct gttcattcac taatgtcgtg taatccggag catctaaagg aactggtgtg
    20401 atcaccgtat ttttaaagtt ttcgtccgcc atttgataga tcatttttcc tttatccgtt
    20461 aggttgtcat tattcactaa attctcattg gctgtcttgc tcgccatgat atcaaaactt
    20521 ttttcgccga atttttcttg tacttctgga gaagaaagat atttgatgaa ttcataagag
    20581 gcctcaggat cttttgctcc gtatggtact tccaagacga atccgccacc ccacgaccaa
    20641 tggcctgaac cttcttcttt ttctgggagc tgcatcacgc caaaatcaaa atcatccggt
    20701 gcattttcca tcaaactaga gtaataattg atattttggg cacgcatggc tactaatccg
    20761 gaaataaatg gatcagatac accagaccca aattcggctt ctaatttatt gatcgtattt
    20821 ttcccataat aatcttgcca gtcttggatc cactccaagg cttccacttt atttttagta
    20881 tcaatcttga ctttttcatc tttgtcaaac caactagtcc cgttatctgc gtttagcgcc
    20941 catacatcag cccctaaatt ccataatgga taaaaaccga tgcgtgtaaa ttcttttcct
    21001 tcttttttat ctagtttacg tgctgtttct tctaattcat cccaagttgt cggtagatct
    21061 tgttcactga ttccagcttc tttaaataac tttttattat aaaaaatgac ttgtgtatct
    21121 gtattgaatg ggatcgcata aggatcacct tcatattcta ctgtatccca aagctgcgga
    21181 taaaaatcct tagaagtgtc tttttctaca tatttggaga gattagttgc ttgctgtgct
    21241 tctgctcttt gggcaacgga atttatgtcc tgtacaataa catcgggtgc atttccagct
    21301 gttattgcag ataaagattt agtccagatg tcaccccatg gctgatactt ataatttaca
    21361 tggatctcgt tttgtgattg attgaaatcc tcaatgattt cttcgatagc ttcttttcgt
    21421 gcaccggatc cccaaaagga ccagaaatca atattttttc ttccatcact tgttttttca
    21481 gaagaatttg ctgcacttga actggcatcg cttgcattac ttgatccgca tcctcctaat
    21541 aaaaacaatg ctgaacttgc cagcaaccct gtaaacaata attttttcat attttacctc
    21601 caaattttaa tgtaaccgat gccggtttta atataacgat atcattttgt tctgaaaata
    21661 acaatttata aggtatgatt ctaaaaaaat tattaatgat accaatactt tatataattc
    21721 gcgagcgctt tctttgaatt ttttaatctt tcgtagcaga atgagaatgt attaaaaata
    21781 gattaggagg aattttgatg tttagacacc agaaagaatt gcagtttgaa gcaaaaccag
    21841 atcgtccgaa tccattaatc gcgaaatatc tgcaggagct gatcggcggc caatatggcg
    21901 agatgagtgt agctatgcag tacctgttcc aaggctggag ctgccgagga gaagaaaaat
    21961 ataaagatat gctgatggac attgcaacag aagaaatcgg ccacgtggaa atgttggcga
    22021 caatggttgc tcgtttatta gagggtgcgc ctgttgaaga tcaagaagca gcgatgcaaa
    22081 aagatccaac aatcggtgcc attatctctg gaatgaatcc acagcacgcg atcgtagctg
    22141 gcttaggacc tcgtcctgct gacagcgtgg gcaatccatg gagcggtgcc tatatcattg
    22201 cgagtggcaa cttgttagca gactttagag ctaatttaaa tgccgaatca caaggaagac
    22261 ttcaagtagc acgtatttac gaaatgatcg atgatcgtgg cgtaaaagat atgctgtctg
    22321 ttctccttgc aagagatagc tatcatcaaa atctatggga atgtgcagtg aaagaattag
    22381 aagaaaaaga aggttcaatc ttagtcccaa gtactttccc gcgtgacaaa gaacgaaaag
    22441 acattgctta tgacttctat aacttctcag agggagacca aagtagtgaa ggttcttggg
    22501 ctagtggcaa agcactggat ggcgaaggcg aatatcaatg gttgaaagaa cctaagatcg
    22561 aaggcgctgc accaaaatta gaaccagtaa caccaaaaat gtatgggaca cctccattca
    22621 agaaataaat atctacccgt agaaacaccc gttttttgtt ccacacgctt tgtgagcaaa
    22681 actgactaaa gtcattaatc ataaccaccc ttcaaaaggg tggttttctc tagagctata
    22741 agctcaaatc accggccagc gcctaaagac gctggctttc atattttatg ttcaagccat
    22801 attgcctttg ccagttttgc ccacctctta aaaggtttat gtactactcc ttagccctta
    22861 aaaggatcgt cttactctcg tacactcagc ttatctaacg cactatcatg tttttcttgt
    22921 tcttgaatgt actttttgat ggtcgcttca ttttgtccta cttttgtcac atagtatcct
    22981 tccacccaaa agtgtcgata cccaaattta tattttagat ttgcacaatc tcttgatgat
    23041 ctcctgaata ttggctcgat attgattata aattattttt cttctatact ttggtgtgaa
    23101 tacaatgtgg tatttacaca tctactttgt acgggctaaa ctatttccct ttattgctcc
    23161 acccgcatag caagtggttt tgtatttcaa tcgctcggcg cgctcaatta ggtctaaaga
    23221 caataaatct aaaaacccgc tgcttcactg acagaaacaa cgggttttca gaaaaaaatg
    23281 aaatgaaaaa gatctcttac ggcaatctat tttatttttt tgatttttta taaatggaaa
    23341 atgcttcaag aagtccaaga aataaagccg tataaaaaca agaacctatg aaatctttat
    23401 ttattagata ttcaacagaa atcccgataa cactagccaa caatatattt atcagaagtg
    23461 taaaataagg atgcttttta tagaatagaa ctagtgttgt tttcatagac tctcctttct
    23521 tcatatttcc aattaatcac aaattcacgg cattactaga aaaattaaag gggcgcagaa
    23581 gtggaaaaat actcttcgac tttccctaca aaccaggcca tcttgtgttt aataactata
    23641 ccaaatctaa ggtacctcta aatctaaatt ccatttgtgg cggtgacctc caatttttaa
    23701 ctcttctatc tcccatccag tatacataac cttgatactt cgaccactta tatatcgaac
    23761 acacaaatac ctatccattg caggagtact acctaactta gttgctactt gcatctaaat
    23821 aacagctgaa aagataggac ttactcttag taaagtatct gaactattaa tttttaaact
    23881 atcattttta tcctcaacta catgcacatc tgttgcaact tcactttctg tctcatccag
    23941 ataaatgatt aaggaatcgg gaattgcaat atcttcagct gacgctggaa tagttgagct
    24001 aaattagtca tcatatcttt attataaaat aaccaacagc aaaaaataca atgaaaaaca
    24061 aacttggaac taatgtatca ttaataaatg ttgaaaaatt cagcaactca ttactgtatg
    24121 attttagcag cccaaccaac atatacccta taaaagctaa taagatggtt aggaatcctt
    24181 tagaatttt
//
LOCUS       DAFBQH010000034        22823 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.034, whole genome shotgun sequence.
ACCESSION   DAFBQH010000034 DAFBQH010000000
VERSION     DAFBQH010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.034"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(579..1595)
                     /gene="tsaD"
                     /locus_tag="LK694_001523"
     CDS             complement(579..1595)
                     /gene="tsaD"
                     /locus_tag="LK694_001523"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBK5052392.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLTLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1592..2056)
                     /gene="rimI"
                     /locus_tag="LK694_001524"
     CDS             complement(1592..2056)
                     /gene="rimI"
                     /locus_tag="LK694_001524"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBK5052393.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2063..2605)
                     /gene="rimI"
                     /locus_tag="LK694_001525"
     CDS             complement(2063..2605)
                     /gene="rimI"
                     /locus_tag="LK694_001525"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBK5052394.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LGVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2589..3311)
                     /gene="tsaB"
                     /locus_tag="LK694_001526"
     CDS             complement(2589..3311)
                     /gene="tsaB"
                     /locus_tag="LK694_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBK5052395.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(3502..4482)
                     /locus_tag="LK694_001527"
     CDS             complement(3502..4482)
                     /locus_tag="LK694_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5052396.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(4506..5990)
                     /gene="galT"
                     /locus_tag="LK694_001528"
     CDS             complement(4506..5990)
                     /gene="galT"
                     /locus_tag="LK694_001528"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBK5052397.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6002..6991)
                     /gene="galE"
                     /locus_tag="LK694_001529"
     CDS             complement(6002..6991)
                     /gene="galE"
                     /locus_tag="LK694_001529"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBK5052398.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            7239..7406
                     /locus_tag="LK694_001530"
     CDS             7239..7406
                     /locus_tag="LK694_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBK5052399.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(7468..9279)
                     /locus_tag="LK694_001531"
     CDS             complement(7468..9279)
                     /locus_tag="LK694_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBK5052400.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(9276..9641)
                     /locus_tag="LK694_001532"
     CDS             complement(9276..9641)
                     /locus_tag="LK694_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBK5052401.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(9804..10199)
                     /locus_tag="LK694_001533"
     CDS             complement(9804..10199)
                     /locus_tag="LK694_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBK5052402.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(10217..11179)
                     /locus_tag="LK694_001534"
     CDS             complement(10217..11179)
                     /locus_tag="LK694_001534"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBK5052403.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(11179..11391)
                     /locus_tag="LK694_001535"
     CDS             complement(11179..11391)
                     /locus_tag="LK694_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBK5052404.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(11412..12110)
                     /locus_tag="LK694_001536"
     CDS             complement(11412..12110)
                     /locus_tag="LK694_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBK5052405.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGELVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12130..12672)
                     /locus_tag="LK694_001537"
     CDS             complement(12130..12672)
                     /locus_tag="LK694_001537"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBK5052406.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(12804..13808)
                     /locus_tag="LK694_001538"
     CDS             complement(12804..13808)
                     /locus_tag="LK694_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBK5052407.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALTSGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(13805..14794)
                     /locus_tag="LK694_001539"
     CDS             complement(13805..14794)
                     /locus_tag="LK694_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBK5052408.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(14791..15597)
                     /locus_tag="LK694_001540"
     CDS             complement(14791..15597)
                     /locus_tag="LK694_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5052409.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            15763..16719
                     /locus_tag="LK694_001541"
     CDS             15763..16719
                     /locus_tag="LK694_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK5052410.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVRKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(16796..17314)
                     /locus_tag="LK694_001542"
     CDS             complement(16796..17314)
                     /locus_tag="LK694_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBK5052411.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(17402..17551)
                     /gene="rpmG"
                     /locus_tag="LK694_001543"
     CDS             complement(17402..17551)
                     /gene="rpmG"
                     /locus_tag="LK694_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBK5052412.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            17779..18225
                     /locus_tag="LK694_001544"
     CDS             17779..18225
                     /locus_tag="LK694_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBK5052413.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(18420..20315)
                     /gene="asnB"
                     /locus_tag="LK694_001545"
     CDS             complement(18420..20315)
                     /gene="asnB"
                     /locus_tag="LK694_001545"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBK5052414.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEVIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(20640..21614)
                     /gene="bsh"
                     /locus_tag="LK694_001546"
     CDS             complement(20640..21614)
                     /gene="bsh"
                     /locus_tag="LK694_001546"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBK5052415.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(22140..22213)
                     /locus_tag="LK694_001547"
     tRNA            complement(22140..22213)
                     /locus_tag="LK694_001547"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22177..22179),aa:Ile,seq:cat)
     gene            complement(22407..>22823)
                     /locus_tag="LK694_001548"
     CDS             complement(22407..>22823)
                     /locus_tag="LK694_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK5052416.1"
                     /translation="TSDDKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIENANLHVAG
                     WHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPS
                     KKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT         6854 a         4812 c         3916 g         7241 t
ORIGIN      
        1 aatatcaggg tcactgctta ttttcctccc taaaacttct gaaagatccg gcagttttag
       61 atcactggac aagactcaaa gattttctat gtgtgtttca gttatgtaat attcactgtt
      121 tgcaaaaaat actgttagat taactagatg aagcgtcttg tcctcatccg atctagatta
      181 ttcgatattc ctcgacaata aacgattact cagtgagggc aatcagagga acggataatg
      241 ataaataagc tgtaaaaaat agcaaaataa caataatgat taaaagtatg taaaaaatag
      301 ggctttgaca aaaacgtgtc acagccctat ttcgattaaa cggtgtgtac ctgtcaactc
      361 ttcggtcaca agtcataatg ttcataaaca tgaggagctg ttttttgaaa ttgttccttg
      421 tgaatcagag ttaaacgaca ggcttacgaa ctcttgatta aacggcgttc aaaagcttgc
      481 atacgcggta ataagaacag ccaggtcaaa aacatgagaa gccgtttttc ctgggctgtt
      541 cttattactt gatgcagggc agctttttca caacctcttt attcagataa aaccaagctt
      601 ggatcagcat tcaactgata gctagcaaaa tttctttttt gcatttccac atgtgctgct
      661 gctccaatca ttgcagcatt atctccacaa agtcgaagag ggggaatcac tagttctacc
      721 tcagggagtt tagttgccaa agcatcctgc aatccttcac gcaacccttg attggccgct
      781 acacctccag ctataatcag ttgcttgact ggataatttt gacaagctcg aagtgtttta
      841 gtaatcaata catcgatcac acttgcttga aagctagctg ctaaatcatt tttgtctaga
      901 tcttctccac gctgttgtgc attatgcacc aaattgataa atgcactttt taatccgcta
      961 aaactgaaat cataattatc ttcatggatc atcgctcgcg gaaaatggta attatctttt
     1021 ccctgatgag ctagctgatc gatttctttt ccgcttgggt aagaaagacc taatactctt
     1081 ccaactttgt cataagcttc tcctgctgcg tcatctcttg tctccccaat gatttcatat
     1141 gatccatctt cttgcatata gactaattct gtatgtcctc cgctaacaag aagtgccatc
     1201 aatggaaact ggaatggttt aaccagacgg gctgcataga tatgccctgc catatggttt
     1261 acaggaataa gcgtcaattg gtgtgcccaa gcaaaagcct tagctgcaga aataccaatc
     1321 agcaacgagc cgacaagtcc tggtccataa gtgacagcga ctgccgataa atcttctgcc
     1381 gacacaccag cttctaccag tgcatcttcc aagcataagg tgatttgttc tacgtgatgg
     1441 cggctagcta cttcaggtac tacgccacca aaacgtttat gactgtttat ttgagaagca
     1501 acgatgttag ataagatctc tgttccattt ctaacgaccg ctacactcgt ttcatcacaa
     1561 cttgattcaa tcgccagaat caattcttca ttcatttcat cgtctccgtt tttaactttc
     1621 tgctcataat gatcgcatct tccgtcggat tgcgataata tgatttccgt ttacctaata
     1681 tacgaaactt ttctgattca taaagttttc ttgctgcttc attagataga cgtacttcta
     1741 aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag taattgtaac aaatgccgtg
     1801 catgcccttt tccttgttct gaaactgcaa cagcaatatt tgtgatctcg acttcatcta
     1861 ttactttcga gtatccaata aagccacaga tctggttatc ttcagctaaa agcagataat
     1921 cagagtgagg ctgttggatg tctgccaaaa actgcatttt cgtccaaggt gaaccatgtg
     1981 gataagcatt ttcgctaatc atccatacgc attctgccaa gtacgcttca tcaaaatctt
     2041 tttttctgca aatcaactaa tctcactcat tttctaaaag ttttatcata tccaacgcat
     2101 cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt tgcttgaaac cccaatttcc
     2161 gataaagtct ttgtgcatct tgattactca tacgcacttc taatgacatc gtctcacagc
     2221 gattcattat agcaaagttc tcgatttcgt caataagcag cgaaccgatt cgtttacgct
     2281 gatatgccgg tcgaacggca atattcgtga tatggcaatc taaaccgaac gctcttgaac
     2341 cgataaaacc aatcattctt ccattatcaa agatacctag atatagatga ggaagcggtg
     2401 acttcaattc tgatataaag gcactttttg tccatggcaa ttcgccatta tagacttctc
     2461 gttcgacacc cagcagctct ttgatatctt ctgtagtcaa ttctcttagt acgtaacgac
     2521 gatcgatttg ttcgactgtt ttttctgtat aaatcatttt atcttgaaaa aagcttctgg
     2581 catacgctct aaattttttc aacataactt tcgtctccag gtgtgtgcgt ctccaaccat
     2641 ttttcttctg cttctactcg ttttaaatag cgaggaagaa aagcttgttt ctctgcaact
     2701 ggttcaacta aagcacctaa ttgagccaca gttacaccac ttggaatatc ccatgctgga
     2761 actgtattaa tcatcgcact aggcaatatt tcctttattt cagagaagaa tttgtgacaa
     2821 tcactcccga cgaaataaat agcttcattc agcttttgta gttgtgtgaa caaatcagaa
     2881 gcagaaatat gtacatcttc aagtacagtt tctaatctat tttctttcca gcggtatgcc
     2941 ccagcgtata cattttttct tcgagcatca aacatgggca caataatatt atccacaccg
     3001 atgcaattag ccgcgatggt tttcagacta gatactccta ctagctcttt atctaatgta
     3061 taagcaatcg tttttgctgt cgttacacct atacgaagcc ctgtataaga accgggacca
     3121 tcagaaaccg cgattcggtc aatgtctttt ggacttattt gaagatctgc aaacaattgg
     3181 tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga tatttgtttg tatttgtcca
     3241 agaatttgtt cgtcttctac cacaccgatc gccaaagttt ggtttgcagt atctattcct
     3301 aatgtaatca tatatttgat cctttctgct atgacattct cgccttccat tgtagcatac
     3361 ttttttattc tgaggatgaa aataccaaag gcaaaactag tacaataata catgattatc
     3421 tgctggcggg caaattttgc cccttcctga aacacaaaaa agctgcggta tcactccatt
     3481 ttcccacagc ttttccatct tttatttcgt tgattctcga ataatcaact ctgtttccac
     3541 aataatcttt cttgaaaccg gtgctttgga attacttaat tgaagaatcg tctccactgc
     3601 tagctctccc atccattcgg tataaacttt tacagtagaa agcgagggcg ttacatattt
     3661 cgctacactg acatcattga agcccatcac agaaatatct tctggtacca ctaaaccttt
     3721 ttcttgaatc gctttcagtg cgccaatcgc taatgcatca ttagctgcaa aaagcgcatc
     3781 aggaacatgc ggattcgttt ttaaatactg cttcactgct tgatatcctg actcgacaga
     3841 aaaatctgct tcgataattg gaccttcgtc gtagcctttt tctgccaaaa cttgttgaaa
     3901 tgccataatt ctgggatctt ttagccgctg atggtttttc ttcgtatatt ctgttccagc
     3961 aagcatagcg atatgttgcg ctttatggga gacaaagtgt tcaatgactt tttgaacact
     4021 tgacttaaag tcgacaacta gagagtcgat tccttgagaa gtgccgtcag aatctaccaa
     4081 aagcaaggca ttttgggatc ctttcatttc taataactga gcttcgtcga attttcctag
     4141 tgcgatcgtt ccatctgccg gaagatcact aatggaatcc cacgtttctt tcaataaagt
     4201 aacgtttgct tcttctgcct ttttttcgat tcctaggcga atagataaat aataaagatc
     4261 ttcaagctct tccgtatcgt cgtaccactg gatcaggcga agcgtttgct tagtatttgc
     4321 tgcattttta cgatgtttcg tataatttaa gcgttctgct gcttcaaaaa ttttttgctt
     4381 tgtggctaag ccaacggaaa gatcaggatc atagttgaga actcttgaaa cagtagcagg
     4441 agaaactcct gccaactgcg caatgtcttt aattgttgcc atttgtctct cctccttttt
     4501 tacgtttata aaatcgcagt aaatcgttca aacgctgcct gtcctttttc atctcgcttg
     4561 aatactccag cgtcttccag tacacgtgca aaaacctgac ctacagattt ttgtacgaca
     4621 tcggtgaccg tatctttcgt caaatcagga tgttcttttt ttaattgttc tgcccacgga
     4681 cgatgatatt cagccatctg gttttcttct cctaataaga aatgctctac ttctactagt
     4741 tctggtttca aacgaggtgg taaaacagca agccccatta cttcgatcaa cccaatgttc
     4801 tcctttttga tatgttggac atctttatgt ggatgaaaaa tcccatcagg atgttcttct
     4861 gaaacattgt tgtctcgaag gacaaggtcc aactcaaagt aattgtccct tcttcgtgca
     4921 attggcgtaa tcgtatggtg tggtgtacca tctgctgaaa aagctctgat agatactgtt
     4981 tcatccgaat atgctttcca tttttccaat actgagttag ctgcttcaat aagcaagttt
     5041 ttctctgttg attgcaaacg gatcacagac atcggccatt tcacgactcc tgcgatgact
     5101 tgcggataat cttctagttt gataagtttt tctatcggtg cttttgccat aggaaattca
     5161 tgacgtcctg cctgataatg atcatgagaa agaatcgatc ctccaacaat tggtaagtca
     5221 gcattcgaac caacaaagta atgaggcaag acttcagtaa tccgaagcag tctagcaaat
     5281 gtttcctttg tgattttcat cgggcggtgt tgttctgata aaatgatcga atgttcattg
     5341 taataagcat aaggggaata ttggaatccc cagctttcac cgtctagatt cattcgaata
     5401 atccggtgat tggtgcgggc tggatagttc aagcgacctc tatatccttc gttttccatg
     5461 caaagcatgc aggtaggata gtctactttt accgcctcgc gttctgctgc gatttgtttt
     5521 ggatcttttt ctggcttaga taaattgatt gtgatttcca gttcaccata ctccgactct
     5581 gccggaaatt tgatgttttt tgcgatggct cttgttttga tgtagtcatt tttcttactt
     5641 aaattaaaga aatactcagt tgcttcctct gggcttttcg aatagtgctg tgcaaagaat
     5701 gcattgacga ctgaaggtgg cggtgtgaga aaatccatta attgcgcttc aaaaatctct
     5761 tgttctgctg gcaattctga aagaacacca ttttctcttg ccttagcgac aagctgatcc
     5821 aataagtcta cagattcttc cttaactggt cttacttcaa tgctttctag agcatcttct
     5881 cctaccatcc ctaaaagacg attctgtaaa tataagcggt ccatttccat ccatccgcca
     5941 gcttcgatcg caagtgtggc aaaatcacaa atcgtttgac tgattgacat atctcattcc
     6001 cctattcttc ataaccattt ggatggctga catgccaatc ccatgctgtt ttgataatcg
     6061 ctttgatatc tgtgtattca ggtttccagc ccagaatctc tctcgctttt tcacttgaag
     6121 cgactaatcg gctaggatct ccagctcttc ttggtgctat ttctgctgga atttcttttc
     6181 ctgtcacttc tctagctgct tctagcattt ccttgactga gtatcctttg ctgctgccta
     6241 aattgaagaa gttgctttca tttccttctt tcaagtattc caaagctaaa atatgtgcag
     6301 caatcaaatc ttctacttga acataatctc tgatacatgt tccatctggt gtatcataat
     6361 cgtctccata aacagccaag gcttttcttt gaccaagagc tacttgtaaa atgattggga
     6421 caagatgcgt ttctggtgta tgatcttctc cgatagatgc atctgctttt gctccggcta
     6481 cattgaagta gcgcaaagcg acatagcgca tgccatatgc ttggtcacac cacttcatca
     6541 ttttttccat catcaattta ctttcaccat acggattttt aggattagct ggtgtatctt
     6601 ctgtgattgg agactctttc ggctctccat aagttgctgc agtagaagag aatacgattt
     6661 tattcacgtt gaactcgtgc atgacttcaa gtaagatttg cataccgtat acgttgttgt
     6721 tgaaatacat caatggtttt tcgacagatt ctcctactaa agagcttgca gcaaaatgga
     6781 ttactccttc aatcggttcc ttttcaaata cggaacgtag aaattcttta tcccggatgt
     6841 ctccttcata aaaatgagca tctggatgta ctgcttgttt gtgacctgtg agcaaattat
     6901 ctaccacagc tacttgatac cccttttgaa ctagctggtc tacagcgtgc gaaccgatat
     6961 aaccggctcc gcctagtact aaaattgtca taaaaaacac tcctttgatt agtaaaattg
     7021 tgtttgttga ttctatataa tttaaaaaac gaagtttact tttatggtga aaacaaacaa
     7081 agtaaaattt cgtaaattca tatggtaaat ttataataaa acatttagta aacattttca
     7141 aggatttttt gcaaaggttt acaaacaaac gtaaaactag cctttttttc tcttttgttc
     7201 cataatagat ataacaaata ataaatgagg tggattcaat gaaaaaattt gtatctggaa
     7261 tattagtagg aagtctggca acagccgctg ccgttgctgg tttggttgct tctgtgaaaa
     7321 aaacagtgat cgatccaatt gacgaaaaag aagcaatgat cgaagaaaac cgtaaaaaag
     7381 caatgcgtaa acgcgtttct cgttaataat tatttaaaaa tacgcatgaa gactttctaa
     7441 aaaacagata agtcatcatg cgtattttta tttgtcagtc aatataagtt gtgctatttt
     7501 atctgcctgt ctcagattcc atggatctgt ataaggtcta gctactgctg cagttagcca
     7561 atccacacct tcaacaggta ttccatagca ataaaagtca gactggattt cccctgaagc
     7621 atcaatcacg cgattcgtac ctcgctcaac atctatagct tctgaatgat aacttcctgt
     7681 attatctgaa aatgaaaaag ggcgaacaag tttgttttcc ttcaaatttt ttatgacagg
     7741 attacttgaa cgagaaaagc ttgtttgtgg caatcgagct tcaatcaaat agcgactgct
     7801 gatttcttta tcttgtagag aaataacata gcggttgtgc ttttgctgaa tatcaaatgg
     7861 cggttcgatc aaatgaaaaa tacccgcatc tatcagtgct gctaattctt tcgtccgaat
     7921 gattggcgga ccgatcgtta aaaagctatt tagatgagta aaccatcccc atagtatttc
     7981 tttgtattcc ttagcagaaa aaacgtgcca ttcaatagcc tgccggattg gatcacgcca
     8041 gtcttttaat gcatcaaatg ttgaagcaaa tgctcctgtg caattgcctt tttccgcttc
     8101 ttttatctgc ccttcaataa atattcggct agcttctgaa aagggctcat tctgataggc
     8161 aagaggagtt tctaaatacg accaatccca tctataaggg attagtgctg gatgctttcc
     8221 gagacactcc tctgaagaga gggagagaaa atcatgctca aaatttttct ttggaatcgg
     8281 caagtgatat tcttcgatga ttttcttgta ataaaacaac tcaacgtctt ttttcagata
     8341 gtcaaaaaat gtttcgccag tcaaaaatcc cgactgatgc cattgctcca ggttttcttt
     8401 tgttagtaac cgtggccatg ccattgctcc gccttgcttt tgatttcttc ctcgagggta
     8461 ataagggagc ccccttcttg atccactgta tacaatcggt tccttacctg atggatgata
     8521 gaccagtttt cccttttcgt tctcgaagcg ccctcctctt gatactgtaa acaatccgac
     8581 ataatcaaaa aaacttaagc ctagacctct taaaaagaca ttttctttag gcggtatctt
     8641 cgaaagatct acatctgcag gattagaagg aagctgatac agtaaaacat ttttctttgc
     8701 gtagtctacc cacactcttt cttcttctgt ctcatcattt tcccagtgac cgcttgctaa
     8761 aacaagctga tcgatagaat agctttgctg atctgtctgt aaaagataac catcttttct
     8821 tttgcttact ttttttacta aagaataaac aaactgaata gaagctggaa attcttcttg
     8881 caactttgag aaaaaccagc gttgatagag tccgtataaa catcgagtag attgctgatc
     8941 tttttccaga cgattgattt cttctataat tagttgctta tttttggtta ccgtctgttc
     9001 gatataatct gtagcaggtc ctttactcca ttgaaacaag ttgggtccct gtgttaccaa
     9061 tcctccactg gacaatgttt catctgtaaa aagcgtcact tgttgagaaa cagaattcat
     9121 taataactcc ggtggctgat tcaaccgcca aactcgacct ccaggaccat tgggatcgaa
     9181 aagaaaaagt tcaagagaat gtctgtcctg attattatgg agaatccttt ctgctacact
     9241 taatcctcta ggtccggcac caatgatccc tattttcatt tgcatgcctc catttcgata
     9301 aattttttca ataaaacagc atggttctga tgttcatttt ttgcaccata aacaaaagta
     9361 atgactggat gttctttcat aagttgactg agtttaaaaa tagcttgctt cttttcttct
     9421 tcgaaaatca attcttgctg gtatttcttt gcaaatgaag caaatcgttc tggtacatgg
     9481 tcaaaagttt tgcgtgcttc attactaggt gcaatttctt tcaaccacag atctaactgc
     9541 gctttttctt ttgaaattcc tcgtggccag aatctatcca cgagtattct atagccatcc
     9601 tctttttcag catcaaggta aacacgtttt aatttgatca tagttgtttc cttctttcta
     9661 ccttttcgct tagcttatat atgtagataa tagcacaaaa taaaaaaaat gaacggcaca
     9721 actatccatt gcgccgttca tgtgatcttg gtcaaaatct aatgattgat tcggttttta
     9781 aagctgtcat acaatcatct gttttatact tttttacttt tgatttttcc agaccatccg
     9841 tcatatccac ccttaagtaa ataaatctct tgataacctg ctttacgtaa tttatttgct
     9901 gtacggatgc tcaaactttt cttttgatcg taaatataga caggctggtc ttttcgaata
     9961 gatcctaatg aatctttcaa aacagtgtaa gggatgtttc ttgcacctaa aatatgccca
    10021 gcatcaaaca catctttttc gcgaacatcg attacttgtg cttttctcat tccttctcga
    10081 aattcttctt ctgtcaaagt cgttgctgaa cgtttggcca ttactcgtag atagacttcc
    10141 caaaggatga ttgcaagcac aatcaatagc aaaatgatat taattaccca taaaaccatg
    10201 taatgcctcc gtttttttaa gcaaatttca aggctaatga ggcagcacca atgacaccag
    10261 cttcattacc aagctctgct aatttgattt ttgttgattc tcttacttgc gggaaagtaa
    10321 attctttaaa atatttctct acacggctac gtaaaaattc tcctgctgcc gatactcctc
    10381 cacctaacac gatacttgac ggatttaatg tattccccaa gtttccgcaa gctaaaccta
    10441 agtagaagca tacgcgatca acaaccatca atgcaaacgg atcgtcttct tgagccattt
    10501 caaagatatc tttacttgtt acttcttctc cgttgtctag cattgctttc agttttgaat
    10561 cccctgcgta ttcttctgcc aagaaacgac ctaggcgtac cacacctgta gcgctagata
    10621 cagtttctaa acatccgcgt ttaccgcaag tacattcgaa cccttcagga tccacagtga
    10681 tatgtccgat ttctcctcca gctccgctca cgccgtgaat caaatttccg ccagcaacga
    10741 tacctccgcc aacacctgtt cctagagtaa cgaatacaac gtctggatcg ttttctccag
    10801 cacctttcca tcgttcacct aaagcagcaa cattcgcgtc attatcgata gcgaatttga
    10861 ttcctgttcc tgcctcgatc agttttttca caaattgaac ttctgtccag tttaagttat
    10921 acgcaccaat aaccgttcca gcttcgctgt ccacgcttcc tggggtaccc atgccgattc
    10981 cgataaagtc ttcggctttc atattatata aattcaaacg atgattgatt gattcaatga
    11041 tttcaggaac gatatgctta ccatcatcca aaatattggt atcgatgctc catttttgct
    11101 gaatctctcc ttctggtgtt aaaattgcaa attttgctgt tgttccacct aaatcaatcc
    11161 caataatctt cttgtccatt acaaatttcc tttctttcgg ctttcttcaa ttcggtgctc
    11221 tctttttaat atgcgccacg ctgtcatgta tgtatcatga tcaatcaaac ggctatcata
    11281 cagattacgc aattcgatag tcattaattc aatgtcatag atgcgtgcac ccacatagat
    11341 aaagatgcca aattttttga acagctgctg tacatcatat aagctctcca tctttaagca
    11401 cttccttcat attatacaag taaaccatat ttttttaaac ccagtaaaat acatattaca
    11461 aaaagaaata caaaaactag tgtagaaaga atcctttggt gtatcgaata gcttgtctgt
    11521 tgtttcggta gacccaaaac attccctaac actaattcgc caattagccc accgacatgt
    11581 ccccaaacat ctacagaggt atccagaaga ccaaaaacaa atgtcaacac aataaataaa
    11641 agaaaatgtc tgactagctg ctggatcgct gggttatgtt taaaatgaaa ccctaagata
    11701 aacaacgcac cgaacaaacc gaatatcgaa gtacttgcac caccagacag aacattcgct
    11761 tcgttaaaag caaagcttgc tgtatttccc attatcccgc tgaacatata aatcaagaaa
    11821 aaacgccaat ggccatagat tgcctcgatt tgctgcccca taaaatacag tatcagtgag
    11881 ttcattacga aatgcatcag tccaaaatgg atgaagattg gtgtcactaa acgccaccat
    11941 tcattgaagt gaacaagata aggaccatac attccggcat aataagggat ctcaagtcgc
    12001 ggaaataatt ccatcatgag gaagaccgct gtctgtatcc ctaaaaaagc ataggtccat
    12061 aacggtcgtc ttaaccatgc acgcaatttt atttgctgtt gataattcat gaaaacctcc
    12121 catttcattt tagtcaataa acaattgttg gatcttctga tcaaagctct ccggttgcca
    12181 ttgttcattc aattgttcac gaaaaacaag gctacatgtt tttttatgaa aattatttaa
    12241 ataccggtcg taataaccac cgccaaaacc gattcgataa ccggctctat tgtagataat
    12301 acctggtaca atgaggagat cgatcatctc cggcaaatag agcttctctg attgtggctc
    12361 aagaacacca aaactcgaac gtacaagaac ggactgttca tccatttcat aaaacgccat
    12421 ctgacgtttc ggcaaagttt taggtacaac tattttcttt tgttcttttt ctgctcgttg
    12481 gataaccggc tgtgtatcca gttcaaattc attcgataaa gtcgtcccta tcacttttgc
    12541 tgacttccag tactcgctgg cgaatagctg tttatagatt cgctgttctt tttccttttt
    12601 cttgccactt ttggccagat ttttcagctt ttcgattcct ttccggcgta atcttacttt
    12661 caattggtcc aaaaactcac ccttccttct ctcataaaat ggtataggaa aaaaagaaaa
    12721 atagcaatcg aactttcaaa taaatgcata aatagaataa aaagaagacc tagaaatgat
    12781 tggccttcct tttttaatga atttcaactg ttcagcgcgt gtttttgtct gataaaaagt
    12841 gcatacagaa aatatggcgc accaaaaagg gcaatcaaaa ttcctgtagg tacttcccca
    12901 cttagtagaa tcactctagc aaccatatct gcactaagca gaaaaatgga accgaacaaa
    12961 ccgctcagaa caaaggtcca gttattttct ttacgtacga ctagcttcgc agcatgagga
    13021 gctagtagtc ctacaaaaga taaactacct gtcaccgcta cactaccgct tgtcaaagca
    13081 acagctaaca acaaaaggat tttttgactt ttagccacgt taaggcctaa tccttgcgcg
    13141 cgttcttgac caaatgatag caatacaatc ctctgtgcat aaagtcctcc tagcggaatt
    13201 aagataaccg cccatggaag caatgcccaa acataaggcc agctcgcacc ccagattgat
    13261 cccgctaacc atgcattcac aaatccataa ttttccttag ataattggat cgtgcatagc
    13321 aatgttaatg cagaaaggcc ggcattaacg gcaataccgg acaataagat tcgattcata
    13381 cccaaaaagc catttcgatc ataggccgca agatagacac agcaagcagc taaaacacca
    13441 cctagaaagg ctacaactag caatagaaac ggcagcgaat agtgcgaaaa gaaacctaaa
    13501 taaagcatca ctaaaaatcc tgctccggca ttaatgccca atatactaga atctgccaat
    13561 tcatttcttg ttactccttg caagatataa ccagaaacgc caagacaagc tcctgctaat
    13621 aaactgatca gtaatcgagg aactcgaaat tctctaacaa tcagcgccaa agaagatgat
    13681 tctttcccta tgaacgtggc aaaaagctga tccaggtaaa taaaaggcgt accaatcatt
    13741 aaactaccta tagacaggat gacaagtaca aaaagcaaga aatagaacgt ccaatatatt
    13801 tttttcaccg ttgttccctc cttacttgta tcagtaaaaa cggaacgcca atcaatgcaa
    13861 tgactaatcc aaaaggtgtt tcaaaagggg gattgatcat cctcgctgta aaatccgcca
    13921 acataaccaa tccaccgcca actaaaaaag agacaggtag aacataacga tagtccgttc
    13981 caaccattcc gcgaacaact ggcggaacca acagcccaat aaatgaaaca ggtccgacta
    14041 acgaaacggc aatggctgct aacactgcaa ctagacagaa aacaatcaac cgcgttcgtt
    14101 taggatgact tccaagagct aatgcatgtg tttcactcaa atgaatcaaa gatattgctt
    14161 ttcctaatga aaaagaaacc aaaacagcaa gtatatataa aggaaacaat actgctaatt
    14221 gtttccatgt gatattggca gctccgccta caaaccaaaa agcaagatcc tgctgcagat
    14281 taaagaattg ggtaagtgct tggctgatag cagtaaaaaa agaactaata gctacgccag
    14341 ctaaaatcag ctgtaaagga ttcatgccca ttttcgaaaa ctgcaaaaaa ccaaatacga
    14401 gcaaagttac agcaaaagaa cctgccaatg acattagaaa cacagaaaaa tcagaagctt
    14461 taggccaaaa agcaaaacta agggccattg ccaacgaagc acccgcgttg attcctagta
    14521 agcctgaatc tgctaaagga tttcttgtca ctccttgcat caatgcaccg ctcaatgcaa
    14581 aacaactccc tacaagaaat gctcccgcca cacgaggcaa acgaatattt cgtatgattt
    14641 ggtgactttg ttttgatcta tcaaaatcaa acaacgcctg ccaaaaatct gcttttccca
    14701 cagtcgctgc gccatttgaa agagcaacaa aaaaaataat caaaagaaca aaacagacca
    14761 aaagcaggag tctatttttc ttcatagtcc tcataattga tcctcctgct cttttggcaa
    14821 caaatcatag gttagaaaaa ctggtttatt tgttcttgga agcgtgacaa tcgttgcatc
    14881 aatctcaaat atttttttca tacactcttt tgtgatcact tcttcaggtg taccttctgt
    14941 gatcaactta ccgtttttca ttgctaataa ataatctgaa aaacgtgaag catgattaat
    15001 gtcatgaatc gacatcaaga tcgttgtttg tttcatttga ttgatttcag tcaataactg
    15061 taggatctct aattggtgtg ccggatctaa aaatgttgtc ggttcatcca agatcaagat
    15121 atctgtttcc tgagcaagcg ccatagcgat ccagacacgc tgggtttgtc caccagacag
    15181 cgttgcgata tcccgcatac gaaatccagc tagacctgta gcatccaacg cccactcaat
    15241 tgcttcatga tcttgcgtgt ccatgccact gaacagtttt tgataagggt atcgtccgta
    15301 agatactact tcttctactg tcatacctgt agggtgttca ctagtttgtg ctaataatgc
    15361 aatttttttt gcaagttctt ttgaactgta tgtagagata tcagagttat tgacaatgat
    15421 ctttcctgat ttcacaggta gtatacgggt aaaacttttc aataaagtag attttccact
    15481 tccatttggc ccaatcaaac tagtgatttt cccttcagga atcgtaacag acatgttttc
    15541 aatgattttt ttgttttcgt agccggctgc aatgtcagct gctcttaatc tcgtcatttc
    15601 acccatcctt tcgctgaaaa tcattctcaa ttatgacgct gtttcaacat ttcgtcaagc
    15661 gctactttca tatctatagc caaatgaagc ggttgacagc gttcgaaata tcaggaataa
    15721 taaccaatga gaaagattct caattatgtt aggaggaaag aaatgaaatt aaaaacaaaa
    15781 gtattcacat tatctagcat attgctgctg gctggagtac tgagcgcttg ctccccctct
    15841 tcagataaag aaacagctac aactgccaat tcgacgactt catccaatag tactcataca
    15901 gtcacagata cacttggaca taaagtagag attccaaacg cgcccaaacg gatcatcggc
    15961 agttacttag aagactatct gattgcacta ggggaaaaac ctatcgccca gtggactgtt
    16021 ggcagtggtt ccattcaaga ttatctgcaa gatgatttaa aggatgtccc aacgatttct
    16081 tatgaccttc cttatgagaa agtgttaagc tttgagcctg atttgctgtt gatttcttct
    16141 tccgctactg tcgaaggcgg gaaatacgaa caatatagca aaatcgcgcc aacttatgtt
    16201 gtaaaaaacg gaacagatgt cacatgggaa gaacaactaa aagatgtcgg caaagctttg
    16261 gcaaaagaaa aagaggcaga aaaaatcatt tccgattatc aaaaatccat caagaaaact
    16321 cgagaagaac tagcagataa aatcgagaac aagacagctg cagtgttgtg ggtgaccaac
    16381 aactcagcct ttatggtcag aaaaaatcgg tcaagtggaa gaatcgtata cgacgatctg
    16441 gaatttggtg ttcctgatct agtggaagag gtctccaaaa aagccacttc tgactggtct
    16501 gctgtttcct ctgaaaagct agctgaatta gatgctgact acatcatcct tgtgaacagt
    16561 gacaaagatg ctgccatgtt caatgaacca agctggaaga atctaaaagc agtgacagac
    16621 aatcatgtac tgcaattcgg acctgaatct agttggctat ataacggacc tattgccagt
    16681 caggaaatgg tcaatgatat caaagaaaaa ctaaattgat tttaactaaa aaaagcatct
    16741 tcacgattag tcgtgagatg ctttttttct tattaacaaa ctagacgcat agattttatt
    16801 cttcttttga agcaacccta ttttttactc tttcccattt ttgaaaacga tgggcatata
    16861 gattttcttg atccgttatc ccctcatact cctctataag ggcgaagtct gaaaaatcaa
    16921 tattcccaat atacgtatcg ccttcaaacg tgctatcgat caatgttcgc cagatccgac
    16981 cagtgtaagg aagaagttct tgaaaaatcc gactaccacc cgaaacatag atatcttctg
    17041 agatcgtttg actatacgac aaaagttcat cgatcgaatg cattacttcc gcattttctt
    17101 tcgtgcgaaa atttggttgc gttgtcaaca cgatgatatg gcgataagga agtgaaagat
    17161 cccccattcc ttcataggtc tttcttccca tcactaaaat tcgatccatt gtgtgttcgc
    17221 gaaagaaacg catatcattt ggcaatcgcc atggtaacaa accatttttg ccgatcagac
    17281 catttttatc ttgtgcccac atggaaataa acatattatc cgctccttat gattttagat
    17341 gttttgagat aaaaaaagtc tcaattcttt gaccagcaag gaattgagaa ctttaaaaac
    17401 attattttgt ttcacggtgc aaagtaactt tgcgttcgcg tgggcaatat ttttgttttt
    17461 ccaaacgatc aggattgtta cgtttatttt tgcttgttag atagttacgt tctttacaag
    17521 aagtacactc taaagtgatg tttacgcgca tgtttttccc tcccattttt acaagaatcc
    17581 tctaagatga ctctcagact tgattatctt accatgattt tttctagatt gctagtgttt
    17641 ttcacgattt tgttaagtaa aactacttga cgataaatac aacggtttta cttctcaaac
    17701 aaaatctttt tctttttgta taaataccct tacacttgtt tatatagatt atgtttttta
    17761 ataaaggggg atactaatat gccatggaac atgaaggact ttcctgcatc aatgaaaaac
    17821 ttagataaat tgacaagaaa aaaagcaatc gacatcgcaa atgcattatt agatgaaggg
    17881 taccctgata accgtgcgat cccaattgcc attgatcaag caaaaaaatg gtatgaagat
    17941 gctagtgaat cagaaaaacg aactttcgaa aaagaaaaaa atccaagcaa gacggatgaa
    18001 cacgatacga atcctcgtgc tggaaaactg cttgattcag atgtgatcgt agaatatgag
    18061 gaagaacaat ggatcgtgaa atctaaaggg gcaaaaaagg caagcaacca ttttgataca
    18121 aaaaaagaag caatcgaaaa aggaaagcaa gtagcacaaa acaaagaatc aactttgttc
    18181 atttataaaa aagatgggac aaaagaaaaa gaaatcgatt attgaacata tgtaaagggg
    18241 ttgtgacgaa aatcgtgtca caaccccctt atgttcgaaa caatggtgtt cctctgccaa
    18301 ctccgaacca taagtcataa tatctataaa acgggaagag ctgttttttg aaattgtttc
    18361 ttatcactca gtgttaaacg gtaggagaaa acctcttttt tattgatatt tgattttcat
    18421 caaacagtca catagtcgat tgcttctgct tgcggaatcg tttcatctac aaagtagact
    18481 ttataccatg tcaagaaact gaagattgtc cagatttttc tttgctcttc tttacgtcct
    18541 tcgtgatggt catctagcaa cttcatgatt ttttcttgat caaaaaattc ttttgcaaaa
    18601 tctgcttcga ataatgcttt gacttgttcg tagccgtgtt cttctttcaa ccaagctttg
    18661 attggcacag ggaatcctaa tttcacacga ttcgaccatt cttccggaag atgtttgttc
    18721 gcagcttgtc tgaaaatatc ttttgtgttg ttttgattca ataaaaactt acttggtatt
    18781 tgttgagcca atttcatgac ttcgatatcc aataaaggta ctcggacttc caaagaactt
    18841 gccatagaaa gtttatctgc ctttaacaag atatcttttg gcatccattg atgaagatcg
    18901 acatattgca tcttattgac ttcatctttg attccttctg ttttctcgta atgaacagtc
    18961 atgatttcat ccacagatgg tgctgtttga tattttggtg ttaacacttc tgatgcttct
    19021 gattcttcga acactcgagc atgaccgata aaatattctt ttgcaggagc aagtgattca
    19081 tataaatgga tccgtccatg gaaatttttc atttttccga ttttacgtcc taaattatag
    19141 cgtgttcctt ttggtaattt tttcaatcct tgagcaatca cacggatgaa ttttgaattc
    19201 gtatggaatc catacgtttg gtatcccgca aacagttcat ccgccccttc tccagattgg
    19261 actacccgca cactttgtga agcaagattt gctaaaaagt acaaaggtac gcaagaagga
    19321 tttgaatcag gttcatctaa gtgatactgg atcaaaggga atgctttgaa cgacatgtct
    19381 ccgtcgatca ctgctgctgt attatctaag tccaatagtt cggttagttt tctggcttca
    19441 atcacttcat tataggtttt atcatcaaac ccgattgaga aagaatgatc tggtttcaat
    19501 acagaggtta cataactgga atcaaccccg ctggataaga aagaaccaac ttctacatca
    19561 ctgaccgtat gtgcttcaat agaagcttga acggtttcgt cgattttttc gatccattct
    19621 tgtcgggaat gcgtttcttt tgtttcaaaa tctgcatccc aatattgctg aatgtctatt
    19681 tttccatctt tatacgtata ataatggcct tcaggcaaac gatagacatc tttgaagaat
    19741 gtttcgccgt ttaatggaga atattggaaa gtcatataag gtttcaacgc ttctttgttc
    19801 aattctttgt taaaatcagg atgaggcaga aaacttttga tttctgaacc aaacataaat
    19861 gttccgttca tttctgcata ataatatggt ttgatcccaa agtggtctct tgcaccgaat
    19921 aactcattct tttcattatc ccaaatagca aaagcaaaca ttccgcggat cttttgcaat
    19981 aattctgttc cccattcttc gtaaccatgc aaaaggactt ctgtatctgc atgtgtttga
    20041 aaaacgtggc cagctgcaat caattcttct ctcaaaggct ggtaattata gatttcgcca
    20101 ttaaaaataa tgatctttgt ccgatcttca ttgtaaatag gctgagtacc gccctctaag
    20161 tcaataatac ttaatcttct gaatcctaaa gcaacatgtt tatcgatata ttctcctgaa
    20221 ctattaggtc cacggtgaac gattcgatcc atcattgtat taataattgt ttttttgttg
    20281 tccttgtcgt taacaaaacc tacaataccg cacatttaac tttcacatcc taatataaaa
    20341 ttactgttta tttcattcat atgcatctta tcatagaata aatagcggac gaatattttc
    20401 tctcttacaa tttcgtcacg tgaaaacaaa agatgaaagt ttctgttttt acagaaaata
    20461 aagtcccaat atctaattta ccatgttttc ttttttgagg ctaccttatt ctcttcgtgt
    20521 taaaaaaata ttagtggatg ctatttccaa aaaaaccagc aaaaaaagac acgacctaaa
    20581 gatcgtgtct aattatgact gtatcagaaa atatgatacc aaacggatct attcattaat
    20641 taattgacat agtttatctg ttgctctttt atgactggat aactgaccaa ttcatgacga
    20701 tccaagtctt ctttattcat atttacagca gtaatttgac tgtcttcata cgtacgataa
    20761 taataaatcc ctttatcaac gttacagcaa gaagaataaa tcgtatattc gtattttctt
    20821 tctccagtat cgcatactcc tttttgttgc tcgactgagc ctagtatatg aaaaaattga
    20881 ctgatacttt cagattctga atcacctgat gcagcattca ttttagtaaa tgtggctttt
    20941 acaaatcgag aaacagaaga gagatctcca ggtaagccca atcctcccat cccacgacta
    21001 taaacattca agctgatctg gtttgaaaaa tgattttctg gtgttccatt tgatagcgag
    21061 cgataattgt tcagattaaa taattgataa tcaaatggcg gattatttgt caacacaccg
    21121 acaggattat catataaatg aagtccatct ttcatacttt caattactat cgatttttct
    21181 ttatcagcta ataaccagtg taaaggtgac aagggtagtt tttcactaaa atcgatattg
    21241 actagattga tagtagccaa taattcttta gcttcgtcta tggttgagca ttgccctagt
    21301 atccatggaa taaactcaaa tggtgcaacg ttatcttttc cttcttgtaa ttctttataa
    21361 tcagcattgc caggaaaatt cagtcctgcc atactcaacc ctttttcatt tgtagcctca
    21421 taatagagtg gatagttgga taccccagct gctattccta tcattgcata atgtttatct
    21481 aaatttttta cctttctaaa atcgaaacga taatttcttg gagtaatagt gaccacttca
    21541 ttataagaca tctcataatc aaaattcctt ccaaaataat ggtctttcgt tgcataagtg
    21601 attgcggtac acatcgtttc ccgtcctaaa acaaatcgtt tttacacttt tatcttacat
    21661 ctagtatttt ttttatcaaa aaaaggccaa agaagattga tttactagca tttataaaaa
    21721 atagtactca gggggaatca aaaaatattg taggttaaaa gtacaacaat aaggggctgt
    21781 gacaaaagtc ttgtcacagc cccttattcc gaacaaacgg tgttcaaaag cttgcatctg
    21841 cggtaataag cccaatcaaa cgaaaaaata ggaaaagcta tttttgtttg gccgttctta
    21901 ttacttgatg caggacagct tcttcacaac ctctaatcgt tgatataagg aaggtacagg
    21961 atttatatat gactggtaat agaagaaatt tactagcata ttttagcgat ttaccctaat
    22021 ttttgcttct agttataaaa tattagtact aactaaattt caaaacgtag tcaaaaacgt
    22081 atttaaaatt ctaatcgggt aagtgttcga caaacggtta ataaactgaa tttatcttat
    22141 gtaccctgta ggactcgaac ctacgaccgg acggttatga gccgtctgct ctgaccaact
    22201 gagctaaggg tacaggtgtg ccacaacaag ccatacaaaa tacaaacgtc tggaaataaa
    22261 tcgtggcaac aaacctgtta tcgcatagct tggagtgtga ctatttatgg gtgatagtga
    22321 agatatgcga taacatcact attttatcga atgattttta tagttgtcaa tatagttatg
    22381 taatgctcct caacgaggag ctatttttat cgtttaggaa tatttaaata ccaacgtttg
    22441 tcatggaaat cttgcgcacc gcctttagtg tttccttctg gatcattcgt tgcccgcatc
    22501 atgacataga ctttcttact aggaaaatta cgcatattga aagatacatg ataaccaaca
    22561 tttccagaag tattataagc ttgatttaca tctggtctat aaattccatc agctcttact
    22621 ctagctagtt cttttccagt attgtagtcc ataatgaaaa tatactcgta tttatagtta
    22681 gcgatgtgcc atccagcgac gtgtaagttt gcattttcaa tttccccaaa ctgatcaata
    22741 tgagcgtagt ttgttccatc tgtcagcgta ggatttgcag cacctgctct agttggatca
    22801 atgactggtt tatcatctga agt
//
LOCUS       DAFBQH010000035        22155 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.035, whole genome shotgun sequence.
ACCESSION   DAFBQH010000035 DAFBQH010000000
VERSION     DAFBQH010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.035"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            349..2391
                     /locus_tag="LK694_001549"
     CDS             349..2391
                     /locus_tag="LK694_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5052417.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            2402..3022
                     /locus_tag="LK694_001550"
     CDS             2402..3022
                     /locus_tag="LK694_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBK5052418.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            3034..3492
                     /locus_tag="LK694_001551"
     CDS             3034..3492
                     /locus_tag="LK694_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5052419.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            3511..3789
                     /locus_tag="LK694_001552"
     CDS             3511..3789
                     /locus_tag="LK694_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5052420.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            3814..5181
                     /locus_tag="LK694_001553"
     CDS             3814..5181
                     /locus_tag="LK694_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBK5052421.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            5196..6341
                     /gene="dgoD"
                     /locus_tag="LK694_001554"
     CDS             5196..6341
                     /gene="dgoD"
                     /locus_tag="LK694_001554"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HBK5052422.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            6368..6859
                     /locus_tag="LK694_001555"
     CDS             6368..6859
                     /locus_tag="LK694_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052423.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            7302..8501
                     /locus_tag="LK694_001556"
     CDS             7302..8501
                     /locus_tag="LK694_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5052424.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(8996..9754)
                     /locus_tag="LK694_001557"
     CDS             complement(8996..9754)
                     /locus_tag="LK694_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK5052425.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            9922..10857
                     /gene="pfkB"
                     /locus_tag="LK694_001558"
     CDS             9922..10857
                     /gene="pfkB"
                     /locus_tag="LK694_001558"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBK5052426.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            10854..12299
                     /locus_tag="LK694_001559"
     CDS             10854..12299
                     /locus_tag="LK694_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK5052427.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            12327..12782
                     /locus_tag="LK694_001560"
     CDS             12327..12782
                     /locus_tag="LK694_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5052428.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            12801..13775
                     /gene="lacD"
                     /locus_tag="LK694_001561"
     CDS             12801..13775
                     /gene="lacD"
                     /locus_tag="LK694_001561"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBK5052429.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(13886..14447)
                     /locus_tag="LK694_001562"
                     /pseudo
     CDS             complement(13886..14447)
                     /locus_tag="LK694_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            14591..15205
                     /locus_tag="LK694_001563"
     CDS             14591..15205
                     /locus_tag="LK694_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5052430.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            15446..15829
                     /locus_tag="LK694_001564"
     CDS             15446..15829
                     /locus_tag="LK694_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5052431.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            16192..16701
                     /locus_tag="LK694_001565"
     CDS             16192..16701
                     /locus_tag="LK694_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBK5052432.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            16801..17451
                     /locus_tag="LK694_001566"
     CDS             16801..17451
                     /locus_tag="LK694_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052433.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            17902..18840
                     /locus_tag="LK694_001567"
     CDS             17902..18840
                     /locus_tag="LK694_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBK5052434.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            18853..19905
                     /locus_tag="LK694_001568"
     CDS             18853..19905
                     /locus_tag="LK694_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5052435.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            20171..20806
                     /locus_tag="LK694_001569"
     CDS             20171..20806
                     /locus_tag="LK694_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBK5052436.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            20997..21788
                     /gene="msrA"
                     /locus_tag="LK694_001570"
     CDS             20997..21788
                     /gene="msrA"
                     /locus_tag="LK694_001570"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBK5052437.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT         7057 a         3509 c         4661 g         6928 t
ORIGIN      
        1 acacaaaatt attgacgctc ccaaaagcat tgtttttagc agtttaatat tattcttttt
       61 caattagact gacttgattt agttacggaa tagatacttt aatgtgttga agaagcaacg
      121 tcccgtgttt atttactaaa gcatccaata ttctaccgaa tatttaatat tcacattagc
      181 cctaaaataa ttgtcatacc atgttacatt tgatataaca gcgttcattt taaaaatagc
      241 tgttagtgtg acaaaacatt aaaccaagtg tgacaaaaaa cgtgatgaaa gctttccgga
      301 aacgtatgtg aacaagtata taatgatttt gtcaggagga tagtcaaaat gaataaacga
      361 attcgtgaaa tactcaaact gattattaaa aaccctgaaa tgaaactttc cactttaaca
      421 agtgaacttg atttgacacg tcgtcaaatc aattacgcga tcaatcaatt taatgaagaa
      481 ttgaaaacaa agaacattcc gttaattcag agaagtcatt ctggaaatat tacggtacca
      541 atcgaggtta tccaaatgat atcacagcct gattacgaca taggtgatga tcaggaactt
      601 gtcgttctca ctgatagtga aagacaagca ttgatagtga tgactctgat cgctaatatc
      661 gattacatca gtctggatca tttggtagat gcggttgatg ttagtaagac gactgttatg
      721 gaagatatca aacgaacgga accgttcatc agaaattata gtctgacaat tcgatataat
      781 cggatgaatg gttaccaatt ggttggttcg gaacaccgta tcttacagct gttatcggat
      841 ctagtaaaac ggtttccgat ttttcgccga gaaatgattc gcagaaaatt ggcacaagat
      901 gtttctgaag aagaaatcgt ccatttgatc cataacatgg aacaaatgtt gcatcttagt
      961 tattctgatg agtcggtaga ctatttgcag acatcagcga gatttttagt aagccgtggg
     1021 atcaagatgt cagataagca cgcgtttttt cccgatgatg tgacggatac tcctgaattc
     1081 cgaatgctga tgatcttggt acatgaaaca ggttggagtc tatccaaaag ttatatggag
     1141 tggttcacgt tattattttt aacttccaat atttttgaga gcaaaactac tcaaaattat
     1201 gattcggatc aagaattgcg aaaattgatc agccaaatgg ttgaaaattt ccaaaatcaa
     1261 acgttgattt tgatcgatga ccgagaagac tttgaacgta ggatactgag tcatctgcgt
     1321 ccagcctgtt ttcgaatacg ctataacttg agtttaggag tttattcaat agatagtctg
     1381 atcaaggata gtaatcatgc gattttgatt gatttgatga aagaattgat tattcctatt
     1441 gagaattggc taaataaggc ctttccaaat gatgaattag atcttttatc ctattacttt
     1501 ggctatcaat taagtaatca taataaattg aataagcaac gacaaagggc tgtagttgtt
     1561 tgtactaatg gtgtcatggt ctcaaagtta gtacgagcca atatggaaaa actttttcca
     1621 gagatccatt ttttagcttc cctatctgtg cgtgatttct acaaatttga agctgattat
     1681 gatctggtct ttactacgac gccactaaaa agttctatgc tgcagtttat gatcgagcca
     1741 atcatgacat atcagcaaca aatcagttta cgatatcgag ttttgagtga tttaggaatt
     1801 actaaggttg atcgagcaat ggatgaattg ttaacaatca ttaggaaata tgcaaatatc
     1861 accaatccag tttcgttgcg agaagaactg cagtactttt tgatcaagga agatggaagc
     1921 acgccgctgg acaacttcaa tgtattgcct tcattgactt attacttgcg gccgagttat
     1981 gtacaaatcg ttgaagaaga gatgtcttgg gaacgagcag tggaggtggc ttgccgaccg
     2041 ttattggaac atcaaatcat tgactcgcgt tttatcgctg attgcttgga tcaaatcaga
     2101 caaccgggat atgcaggtta cttgggaatg aagacttgca ttccccatac aacggtcgat
     2161 aaaggtgtaa tcagtgacgg tgtcagcatt ttagtttgta aaaagccgat acgatttcct
     2221 aatggtgaag atgtcagctt gattttgccg ctttcatttt ttgatttaac gaagcatctg
     2281 cgagccatca atcaaatcgc tgatatcgcc aaagatgatt cattgattga gcagttattg
     2341 attgctgatg acgaaaagac agtttatcaa ctattaagac aaagatctta aggaggaaaa
     2401 catgagacta gcagattatc ctaaattgac agtgatcatg cgtggttata cttttgatca
     2461 ggcagatgct attttacaag caatgcagga atttgaccat caatttgccg tagagatgac
     2521 aatgaacaca aaaggagctc ttgaaaatat acatgaacta aatcaacgtt atggtgacac
     2581 gacttatatc ggcgctggga cggtaaggac attagaacaa gcccaagcct gctatgaggc
     2641 tggtgcaaaa tttttattag gaccacatat gttcacaaaa gaaatgttgt cttacgccaa
     2701 taagaaagga ttattggcgg tacctgccgc aatgactcct tcagaagtcg accgtatgtt
     2761 taaagaagga gctgatattg taaaagtatt tcctgcagcg gttgttactc cacgtttttt
     2821 caaggatatt caagcacctt taggtaagtt accactaatg ggggttggtg gaatcagtaa
     2881 ggagaatgcg aaagagtttt ttgaacaagg tgctagctat ttaggtcttg gttccggtat
     2941 gttcaacaag caagacatcg aagagctaaa tgttaaaaac ttagcacgtt cgatgaaaga
     3001 attaattgag gctattaaat aggagtgagg actttggaac tttatttgaa tgaggaactc
     3061 gtttttcgaa atgttcaagc aaaaacagat agtgaagtct tagcgttttt agcaagcgag
     3121 atgtacaaaa aaaactacgt gaaggaggaa tatattcaag caatccaaga acgggaaaaa
     3181 gaatacccta ctggattacc atcaacacca ccaggaatcg caattcccca tgcaaactat
     3241 gaaatggtca ataaaacaac tttagcaatt gcgactctta aagatccggt cctatttcat
     3301 aatatggaag ataacaatga gcagttacca atccaaattg tgatcatgat ggcgattggg
     3361 gaaccacatg ggcaagttga aatgttacaa aaaattgttg gaatcataca agatgagcca
     3421 ctgcggcaag aaatgatcag agcaggcaat gatactgaat tattagaact attaaagaaa
     3481 gcggtttttt aatataagga ggaaatcata atgaaacgaa ttttagtagc atgcggaaat
     3541 ggtatcgcca cttcaacagt agtagcaaca aaagttagag agtatttgca acaacatggc
     3601 attgatgttt caacgactca aacaaaattg atggaagttc caggaaaagt tcaagactat
     3661 gatctattgg tgacgactgg tcaatttgat ggtcaaacag gtggtgtacc agtaatcaaa
     3721 gggatgccga ttttaacagg tatcggtgcc gatcagacaa tggaagagat tttgaactta
     3781 ctgaaataat agctgatcaa aggagctgaa aaaatgaaaa tcattatgaa cgtctttaac
     3841 ttctttattg atgctggacc aacagttatg ttgccagtca tcatcacgat tattggtttg
     3901 atttttgggt taaagatttc tcgtgctttt aaatcaggtt tgacattagg tatcggtttt
     3961 gccgggatca agttgatatt ggactttatg acgaccaatg ttggaccagc tgccaaggcg
     4021 atggttgatc gaactggtgt taaactagat gctttggatg ttggctgggg ttcgattgcg
     4081 gctgttactt gggcatcgcc aatcattcca atcttgattt ttgccattct attggtcaat
     4141 atcgttttgc tgattttgaa acgtacacat acgctggatg ttgatatctg gaactatcac
     4201 catatggcaa tcgttggtgt tatagtgtat tttgtgacta agaatgtctt cttaggtgta
     4261 ggtgcatcag tagtgatggc aattgcgact tttaagattt cagactggtc gcaaccgatg
     4321 gtagaaagtt tcttcggaat ccctggtgtt tctttaccaa ctgtttctgc gctttcatca
     4381 ctggttattg cttggccgtt aaattggctg ctggatcgta tcccgttatt cagaaaatca
     4441 aaattcacca tcaaagatgc acaaaaatat ttgggatttt ttggtgattc tatgatcatg
     4501 gggttagtta ttgggatggt gattggtgtt ttagccggct atgatatcaa agcggtttta
     4561 caattaggtg tcagcatgtc agctgttttg gttttgattc ctaagatgac ttcattattt
     4621 atggaaggat tgatgccaat ttcagatgct gcacaaaaat ggtcacaaaa aaaattcaaa
     4681 ggtacacgtt tatttatcgg attggatgct gcagttgttg taggtaatcc agatgttatc
     4741 acaacagcat tgatcattat tccactaacg attgcattag ctttagtatt gccaggaaac
     4801 cgtgtgttac cattcgcaga tttagcggtt gttcctttcc gcgtggcaat ggtcgttgcc
     4861 ttgactcgtg gtaatttatt aaaaaatatc attattggct tagttgtgac agcttcattg
     4921 ctatggtgtg ggtcagctac tgcgccaatg ctgacagcaa ttgccaaatc ggttggtatt
     4981 aacttaggaa cttcttcaat gttaatctca tcatttgctg cgacttccat gatccaaagt
     5041 tatttggtat ttatcgcatt tgcttacaaa ccaatcatcg gtattccagt gttgttatta
     5101 gcctttgcgg gcgcatggta ttacttcgat ggtatcaaaa aagttaatca tgaaatggat
     5161 gaaccattga aggcacaata gtaggagggt ttaaattgaa aatttcagat gtcacagtgt
     5221 ataaagtaaa accaagatgg atttttgtta agatctcgac tgacgaagga atcgaaggct
     5281 ggggagaaat gatctcagga actaaaacag aaacggttgt tgctggagct tatgagatag
     5341 gaaaccggct gattggacgt aatccgtttg aaatcgagcg cctcttccaa gaaatgcatc
     5401 gatctttctt ccgaggcggt cctattaatg gaaccatcgt ttcgggttta gaaatggctc
     5461 tgtgggatat caaaggaaaa gcgtttaatg ttccggttta tgaattattg ggcggtgcag
     5521 cacgtgataa aatcaaggtt tattcttgga ttggtggaga tcgaccaagt gaagttgcag
     5581 aacaagcaca agatcggttt gatcggggct ttacggccgt caagatgaac gcgacagaag
     5641 agctgcatta tatcgattcc taccaaaaag ttgatgaagt ggtggaacga gttgcgtcta
     5701 ttcgcgaacg ttttggtgat gcaatggata ttggtgttga ttttcatgga cgggtccata
     5761 aaccgatggc aaaagtgcta gcaaaagctt tggagccatt ccatccaatg tttttggaag
     5821 aagtcgtttt acctgaaaat gaagaacact ttaaagaagt agctgattct gtggctgtac
     5881 cattagctac tggtgaacgc ttgtacactc gttggcaatt caagaacctc tttaaacaag
     5941 gtactgttga tattatccaa ccagatgtgg ctctttgtgg gggaatcttg gaaacccgta
     6001 aaatcgcagc gatggcggaa gcttacgaca tggctgttgc gcctcatgcg ccatatggtc
     6061 ctgttgcact agcagcaact ctgcaagtag actcatgtac gccaaacgtc tttatccaag
     6121 aacaaagctt agggatccac tataacaaag gttttgatct gttagatttt gtgaagaaca
     6181 aagaagtttt ccaatataaa gacggctacg tagatctacc ttcaaaacca ggtctgggct
     6241 tggaaatgga tgaagacaaa atcaaagaaa ttgcacaaga aggtttgatt tggaccaatc
     6301 cgcaatggaa aaattatgac ggtacgatcg ctgagtggtg ataaatgaaa ataaaggatg
     6361 atgaaagatg gcagaagcac ttatgatcac agttttttta ggactgctga ttattttgca
     6421 aataatcgat caaaagaaaa ttatcgtgtt tgctcagagt agtcctgtaa ggaagagttt
     6481 gagtatttta ctgacagtcg ccatcctgtt gattttctgg ccggaacatc tgactgatca
     6541 gattaaactg gttgcttttg cttctgtcat tttgatcaat ggttttttga aggagggtct
     6601 agcaaaagat tctgttgtta gatttggagt attgaacggt aactttaatc gatatcctca
     6661 agtccaagta gaaaaactag gtgacgataa atcatttatt tcattttaca aaagcaagaa
     6721 taattctttt tcaatgattt ttgataaaga tcaaactgaa atgatggatg tctttcaaga
     6781 acttgatatc cattccaaaa ttactttaga tcaaagttcg gaagaaaaaa gagcagttgt
     6841 aaaaaaagat aatgaatgat tctactaccg aaattatgat ttctttatca cgaataatga
     6901 aacttgtttt aatgaatcat attgattgaa gtatacaatg tatattaagt tttggaaaga
     6961 aatatattgg tacaagatat agcaggaatc gattactcga tccctgcttt ttttaacttc
     7021 ctttgcagca aaaaaagctc gtaatctaag agatgatgga tagtttttag aatatatata
     7081 ttttcttcaa ttccagaaaa aaatgaccaa gctagtatgc taaaaaagta cgatgcttct
     7141 atgatctttt ttccgtcttt attaacagat gttttaattg atcaacgcat attgtaatct
     7201 accctaattt ccatagttct tcaccacttt taaaaatagt aaaaccccta caagtatgct
     7261 atagtaaatt gctaaagaaa aaccaataag gtgggagatg tgtgcgcgat caaacaagcg
     7321 gaaaaatgat tcgtttttat tatacaagtt tgcattttct ttattggatg atgttttgtt
     7381 ctgtctatgg gtttgcgaca ttgtttttga tttatttcaa gattgatcca gctaaaatcg
     7441 gagggatctt agcattggtc aatattgttt caacggtgct tcagcctttt gtcagtcaag
     7501 tgatcatcaa gaaaagaaag atcccactga tcaccgtgct gaagacgatg atagcaggat
     7561 taggagtgat tctcttagct ggggtattga ttccgaattt gccgatcatt acttatatct
     7621 tgggtgggat tttagtagtt tctatgcaat catttctcaa tgcgctggca tttgagtatt
     7681 caaataccgg ctacaggatc aactttggct tttcaagagc tggtggttca ttttcttatg
     7741 ctgtaacttc ttttttactg ggacagctgt tagcgattta ttctgccgcg attttgccaa
     7801 tcttggttat agttgaagca gtgatatttt tcgttatttt gcatctgctg ccggatgtaa
     7861 aaaatcaaga agtttttcct atgacaaaag aacaggatcg ctcagtttct ttgagaaaaa
     7921 aatatccttt cctgattttg ttatttttag gtttcagttt tctttttact ttccacacca
     7981 tgatcaacag ctttttggca cagatctttg aaaatatcca cggcggcagt aaagaagttg
     8041 gcatcgcatt gatgatcgcc gctttatgcg aattaccggg aatgatcttt tttgaacagc
     8101 ttgtcaaaaa gaaatccagc aaattttggc tgtctgttgc cagtatttca tttgctgtca
     8161 gaggattcat gatgctgctg gccagttcga tcctgatgat ggaaatgacg catctcctac
     8221 aagccttcag tttcgctttg tttattccag cttcggctta tttgttcaat caatttctgg
     8281 cggatgatga tcgtgtattt ggacaaacga tgatcatcat cgccacaact cttggtgggg
     8341 tgataggaaa tgtcttgggc ggcagtctgc tgcagcattt cggtgtatgg gagatgctga
     8401 ttttcggtac tggttttgcg gtgctaggtg ctttattaac tattttgggc attcgaaaaa
     8461 taccgaagac aggaaacgat agtgattctc ttgttgagta gcataaaaat acttagatac
     8521 tagttttata taaataacat atagtgatta ttggcgtaat tacaaaaaac gagaataact
     8581 taagtaagca tttctaaata attgagaaac ctcatctaaa aagataagaa aatatcccta
     8641 ataaaacttc tgtaacattt tagataactg attgtaatct gaataataaa ctataaggag
     8701 acggttagac ggtctccttt ttttgcgtat tataattcta gaattgttgg aaattgataa
     8761 taattaacaa gaaatattag acaggaactt cgaactgctt atcacattct tattattata
     8821 tatttgcgta agtagtcctg gtagtctcaa tgcactattc tatcttgaaa ctaagaagtt
     8881 atagaaggag caatgataga aagcacgaga atacaaatag cgattaaata tttttctaga
     8941 caaccttagt taattcacac agatatattt taggtacagt cttacagata tagagttagt
     9001 gctgtgtgaa tcctgatgtt cgcagtgcat aggagtcaag taagattcta gccagtatag
     9061 aaagtggaag tctggagcgt acctcataat ctgggaagaa ggagccttct gatttaaagc
     9121 caacaaaagg aatctcgata tgttccatca aacttgaatg acgattagca gtaagcaaaa
     9181 ttgtcccgat ctcagcttta taacagtttt cagctgcggt agtcaattcc tttgtttcac
     9241 cgtttagtga aacaaagatc acgatgtcat ctttggataa ttttctacta atgtttttta
     9301 tgatattggg atcagtatgc atctcacaat atttaccggt aagttgaaat ttgaccatca
     9361 tttcctgagc aatcaattct gaaaaacctc gggcaaaaat catgattctt ctagcagcct
     9421 tgattcgttg aatagcatct tcaatattcc cgctttctac catattgatg gtgcggatta
     9481 cctcttgctc atttttaaga atagcagtct ttattccttc atcaacttta tcaagagcgg
     9541 caaaattgat atttgtattt tctttttctt ttaacgaatg tttaaaagac gtaaacccct
     9601 catatccttt tttcttcata gtacgaacga cggttgctgt tgaaacgttg gcatattcac
     9661 ttagttttac aatggaatag gaggggattt cgagtatgtg actttgaata aaatcccaga
     9721 gatatttctc cgattcactt aattttgtat ccatattttg cctccgatta gttaggataa
     9781 ttctattgta aagcgggagt ttcctttact tcaagtttat ttaatatatt ttgtaaaagt
     9841 tttcaatgaa tttagtcatt tatgaaaaca tttacattcg atcttgatac ataatgaata
     9901 tgtaagaaag gaggagcgta aatgtcagtt tatacatgta ctttaaactt ggcaatcgac
     9961 ctttttatcg aaacagatga aatgaaacca tcccaagtca atcgaacaaa cgatgatgat
    10021 atacaagcta atggaaaagg tgtgaatgtt tcattgatca tgaagcaatt aggtatcgat
    10081 agtacagctt taggatttaa agcaggtttt accgggaatt atattgagga ttatttgcat
    10141 caaaaagata ttagcactga ttttattagc gtaccgggaa tgactagaat caacgttttt
    10201 acacaagtta ataaaaccaa tgaagaattc aaattggtta ataaaggacc tgagattcct
    10261 caggaacaaa tcaaaacttt tctaaatcaa atacaaaagc tgcaagctga cgattatttg
    10321 tgtgtttctg gcagtttgcc gcgaggggtc tcacccgaaa ttcttattga tatcagtcgt
    10381 atttgttttg aaaataaggt ccatctgatt attgattcaa gctatccgga aataattgat
    10441 tgtctgccgt atcagccgtt cttattaaaa ccaaacgagg aggaattgca atcttggtat
    10501 aatgtcgagc ttagtgatcg tgaaggatat attcattatg ctgaacgctt gattgccgat
    10561 ggagcgaaaa atgttctcct ttctctagga ggagagggag gaatattttt aaataaggat
    10621 atcgtttatt ctgggaattc tcctaaaggt aaagtagtca atacagcgtg tgctggtgac
    10681 actttgttgg gaacatttct tgcaggatat ttaaaaggtg aacaggtaga tattaatttg
    10741 aagaaaagtt tagcagctgg aagttcaacc gcttttcgta aaggattaac agatttttcg
    10801 gatgttgcag agttggaaaa acaaatcgaa atcaaagagg gagcgagtaa aaaatgacaa
    10861 cttacaaaat tgttgccgca acaggatgtc caacaggaat tgcccacact tatatggctc
    10921 aagaagcatt ggagcaggca gctaagcgca aaggggtatc tatcaaagtt gaaacccatg
    10981 gtcaagtagg gatagagaat gaattaacac cagaagaaat aaaaaacgct gacgctgtaa
    11041 tcattgcagc tgacaaagat gttcaagcag aacgtttcgc aggtaagcgt gtaatcgatg
    11101 tgcctgtcag caaggggatt aaagatgctg accaattgat cgaagatgcg ttggctggta
    11161 aaggttcaat cactgcagaa aacaaaacag ttgacgctgc tggtgagaaa gcggagatgg
    11221 gggttgcagc aggcattggt cacagtattt ataaaaatct catgaacggt gtttctcata
    11281 tgctgccgtt tgtagtggca ggtggggtaa ttattgcaat ttcgtttgcc atctggggtg
    11341 tgtactcttt tgatcccaat gatcctactt acaatccgac tgccgctatg ttaaagggtg
    11401 ttggtgatgc ggcaatggga atgatggttc ctattttagc agcttatatc gctgaaggaa
    11461 tcgctaaacg tcctggtttg gttgttgggt ttgttggcgg actgttagcc aattctggcg
    11521 gagccggttt cttaggcgga atttttgcag gtttcttagc aggctacttt atcttactat
    11581 tgcaaaaggt catgaaaggc ttgcctaaac aattggatgg tctgaaggcg atcttcttat
    11641 atccggtgat cggtgtcttg tttgttggtg tagtcatgtc tttattagca aatccaatgg
    11701 aagcagtcaa ccaaggaatg atgaatttct tggcaggatt ccaaaattct aatccattaa
    11761 ttttagggat tatcgttggc tgtatgtcag catttgatat gggaggtcca gtcaataaag
    11821 cggcttatgt tacagggact gcgttgttgg cacaaggtaa ccatacattt atggcagggg
    11881 tttcggcagc ttgtatcgcg cccccattga ttactggatt tgccgcactg ttttttggaa
    11941 aatatttcga cacgaatgat cgcaacgctg gtttagtcaa ctttatcttg ggctcaacgc
    12001 acattaccga aggagcaatt ccatttgcgg ctaaagatcc gctgcgtgtt ctgccaacta
    12061 tgatgctagg ttcatcaatt gctgctgtac tgacatatat gtttggtgtc caagttcccg
    12121 cacctcatgg aggttttcta gtattacctg ttgttactgg taaggttgca tgggtagggt
    12181 cgattttact tggttcaatc gttggcggag taattttagg gttgatccaa aaacgtagtg
    12241 tagaaaaacg tgaagcagaa gcagctgctg ttgaaatgaa agaagcaacc aacaactaaa
    12301 ggttattcta gaaagaagga tttcaaatga ctttgatcaa aacaaataaa gtttttatga
    12361 ataaaaattt tacgacgcaa gatgaggtaa tgaacttcct tgcagatcag tcaattgtct
    12421 tgagcattag tgaagaccga gatgcagttt acgaaaaatt gatggaaaga gaagaagaaa
    12481 gcacgactgg tatgatggat ggttttgcca tccctcatgc caaagatgct agtatcaagg
    12541 atgccgatat tgtgattgtt cggttaaacg gtaaggtcga ttggaaaagt ttagatggag
    12601 aaggaacgga ttttgtaatc gctctgttta ttccagattc agaggctgga acaacccatc
    12661 taaaattttt atctcaggta gccagacttt tgatgcacaa agatgtgaca gaaggtctga
    12721 aacaagccca gtctgctgaa gaaatagctg aacttttaaa ccaaaaatta gaagaaaact
    12781 aaaaaggaag tagtgaaatc atgcctttaa cagaaaaaaa gaaaacttat attcgtcaac
    12841 taagtaatga aagtggcatc atcagtgcgt tagcaatcga tcaacgtggt gccttgaaaa
    12901 aaatgatcgg taaatttcaa aataaatcag caaccgatga acaaatcatc aatttcaaaa
    12961 aaatcatttc ttctgaattg actccatatg cttcagcaat cttattagat ccagaatatg
    13021 ggttgccggc agccgaagtt cgcgataaaa atgctggtct gttgttggcc tatgaaaaaa
    13081 ctggttacga tgcttcaact ccaggacgtt taccggatat tttatctatt tggtctgtaa
    13141 aacgtctaaa ggagcaaggg gccgatgctt gtaagttctt gttatactac gatgtagatg
    13201 aatctgatga catcaatgac caaaagaagg cgtttatgga gcggattggt tctgaatgtg
    13261 ttgctgaaga tttgccattt ttcttagaat tggtttctta tgatgccaac gatcctgata
    13321 cttcttcgaa ggaatatgcc ttgaaaaaac cgcataaagt aaatgaaatg atgaaggaat
    13381 tttctaagcc tcgctatgga gttgacgttt tgaaagtgga agttcctgtt aatatgaact
    13441 atgtggatgg ttacactaaa aatgaagttg cttataccaa agaagaagct gctgcttatt
    13501 tcaaagaaca atccgagact accaacctgc catttatttt cttgagcgca ggagtaactg
    13561 ctgaattgtt ccaagaaacc ttgaaatttg caaaagaggc tggctctaca ttcaatgggg
    13621 tactctgtgg gcgcgcaact tgggcgaatg gtgtagaacc attcgttact aaaggtgaat
    13681 atgctgccaa agaatggatg cagacacaag gacgcaagaa catcgaagag ctgaataatg
    13741 ttttgaaaga aacagcaaca gctattaaat tgtaagaaag ctttaactat aaccttaagt
    13801 ttgtaaccta atgattttta ataatcaatt tgagctttta aataaagaat tggaagcgtc
    13861 ccgacgcttc ctttttcgtt tgtttttaag gcatgatagg ttctatgttg tcgttcaata
    13921 agcgcaagaa ggtcagcatt tttttcttag agtatgcaaa attgcttttc tacccaatga
    13981 tttaaaacgg agaggcaccc ttggatatgg tagatggtga agtaacgcat ttcttctgtg
    14041 atcacggttt ttttgtcttc ttgtagccag atttttaaat aattttcttc gatcagatta
    14101 tttaatctct tttgaaattg gatatctccg tggttgatga cgaataactt gtatacgaac
    14161 gtattcgtat agacatagtc gatcaattga gcgtatagat cctcgtagct atgtgtcgta
    14221 tcgtaggaaa tgatttcatc caagtcttgg aaaaagctat tttccagttt ttcataaaga
    14281 tcatagatat ccgtataatg tgtataaaat gtcgaacggt gaagatcagc agtttcggtc
    14341 agttcacgga tggtgatgtt ttgaattttt ttatctacta ataattttga taatgcttct
    14401 gctaacgctt tttctgtttt tttgacgcgg cgatcctctt ttttcatttt gcagcctcct
    14461 aatcaacata atcaaggaat tgtgtcggtt atccgacaat ttcaccgcta tctgaacata
    14521 gataaccgac accttgtcta ttataatata agtatagcat atcaatattt aaagaatagg
    14581 agaataagtg atggaattag cctttaagca actggaaaag aaagactaca aaaaagccat
    14641 tcaattcgcg attaaaggta tgcactttga ttggtatacc aagaacaaac tccttttgaa
    14701 tctttatgga agatactttt ggtatctaga attgaatcgc gcgacacaaa tcattgcttt
    14761 atatgcggat gatgagttgg caggtgtttt attagcagac atcaaaggaa aatcaaaaaa
    14821 gcatcattca tttagtcaaa agttatatgt taaactattt gatttcttac aaaatgcatt
    14881 tgtgaaagac agcaaaggcg ggtatgacga caccaatgac gaattgcttt cagactattt
    14941 aaaaaaacat tccccagatg gagaaattgt ctttttagct gccaaccctg atttaaagat
    15001 caaggggatc ggtagtaaat tattaaaaga atttgaacgc cgagagcaag gaaaagaagt
    15061 atttctgttc acggatagcg gctgtacgta tcaattttat gaacgacgcg gtttcgaacg
    15121 gaaaaaagaa aaagataacg tcatgaagat ccttaataaa gaagtagatc tccgctgtat
    15181 gttatatagt aagatcattc agtaatgttt tttactatca gaaagcagaa gtaccacgaa
    15241 ggtcaatgga aaaagcgagt aattgatcca tttagatgaa gataggtgta gtctcaaaga
    15301 tattagctgg caaagatatc cccagttaaa aatagatttc tgtatccaac aataaaaata
    15361 tttttgtatt tattagtata tatactttac aaaaatttgt atatatacta atttttttgt
    15421 aggatcaatg aaatgagggg aaaatatgcc aaaatataaa gaaatagcct tggctctaaa
    15481 agagaggatc gttgagggaa aatatcagga aggttctaaa ttacctgagc aagaaagtct
    15541 ggctagagag tttaaaacaa gccgtgttac tatccgcaaa gctatacaac tccttattga
    15601 tgaaggtttg ttatatacga gacgtggatc tggtactttt atacgaagca atatcagaca
    15661 ggacaatgag aatgtcactc aagtaaataa tgtatttggc acttcctcac aagaaaatac
    15721 aaatgtgaca agtaaaataa tccgttttga tgtccggttt ccgacagatt atgaaaggcg
    15781 gctttctgca acctacttcc gatccccttc ctgcaaatca tgggtataaa aaataggcat
    15841 attaagcggg ctaggaggag aaatattcac atatcatttt ttcattccgc aagctgcgtc
    15901 aagttatttg gaatttgtag aacagatgag agaggtagaa gcagcactac aaactacttt
    15961 tcaataagta ggaattatag aaattttact caaaaaatct tcattaaaaa atctgactaa
    16021 ataagttgag aaaacagaat atacttcaat atccagtgat ttataaatat cattcactca
    16081 tgatacccag ctccgtatca taagtgatgc ggtttttatc attgatccta aaaatcttag
    16141 caagacaaaa agtacgtaag aaagtaaaga ctaaatactt cggaggaaca aatgaataac
    16201 aatgataggt tattgcgatt gcgttatgcg attgatttga aagatacgga tttgattaaa
    16261 gcttttgagc taggcggtgt accactgact aaagaagaag ctcaggcggt actgaccaaa
    16321 gtacaggata agaacaaaga taatgcagaa aataatgtgt atgaaaaaac catcaacaac
    16381 caagtttttg acgcattcct aaatggcctg attctcttgg cgcgtggtcc gcaaaaagat
    16441 aagccgatag tagacacgcc gcaaaaaagc aaggaaatca aatatatcaa caatgtgttg
    16501 ctaaaaaaat taaaaatcgc cttgtctatg accactgatg atattttaga agtttttgct
    16561 gaggcagaac tctatccatc aaaaggcgaa atcggggcat ttttgagaaa agaaggccaa
    16621 cgaaatttta agccttgcgg agataaatat atgcgtaatt ttctcaaggg gttaggaatt
    16681 tataatcgga gaaaagtata aagaaatttc atgagaaatc aaaacaatgg ttgttttgat
    16741 gagagctagt ttttgtatga gattaaaata ttatcttaat cgaggagctg gattggtttt
    16801 atgaatgatg aatataagaa tgatgaagat aagatgcttt ttgaagagat agagaatcga
    16861 tgtaggctca attttgagct tcggggaaag atgtctttaa tacaacaaaa aaagtattta
    16921 gcgaataaat cagagtttac tctgggacat gttgaaaaat taatctcgga ttggattagt
    16981 agtagaagcg aatttacaaa aataaaacag ccgattaaat ttgacatgaa aaaacttttg
    17041 ttaaataaga gtgagatagg taatagagat caatatatta gagctaaagg gcaagaaata
    17101 attgattcat taggtgaaat gaggtcatac aattatttgt atgtaacaca tagagctgat
    17161 ggaatggtca ttactgtggg gaaatcttct tctaatgata tttttttaga tggagatctt
    17221 ttttatcaac tcaatacaaa tcacttgtct ggcactgaaa atataatttt aaggacggaa
    17281 tatggcaatg aaatatttgc aaaatatgat gagattttaa aaaattattt ggactgggct
    17341 tggataattc cggttgagtc aggagatgct aagaaactgg agagattatt gggcgatgaa
    17401 ctaattaata aaaaagtacc aattttaaat tattattccc atagacaata aaaggctatt
    17461 tttcatgata atactagtta taactgtaaa aatggaagaa aatctgggaa agaagttttt
    17521 aaataagtgt attgttcgat tttaataaat aataagcaat accatccgtc tgtgttgcgc
    17581 aacatcgtac tacaatgaat attttttcac aaaaagaaaa tcatccatcg aatgaatgga
    17641 aggttttagt atacacgagg ttaatgcccc ctcttttata atcagaggag tgaggttttt
    17701 tctatgaatg tttctatttt cttattgcta gtctagtttc tcttacgatg aaattgggat
    17761 tctattccag tttttattga tttgctgata aaataacttg aatggtggat ataaacatga
    17821 taaataagag aagaataaca aaacaaggtt gtgaaatgat tctcaaatga atataataga
    17881 aagaaaaagg aggaagaatc aatgaaaata gcaattgcag gcgctggcgc aatgggaagt
    17941 cgctttgggt taatgcttca ccaaagtggg aacgaagtat tattaatcga tggctgggca
    18001 gaacatgtcc aacaaatcaa agagcatgga ttacaagcaa attttaatgg taaagaggta
    18061 gaagcaaaac taccaatcgt tcttcaatcc gaagtagaaa aagaagatca agttgatctg
    18121 attattctat ttaccaaagc gatgcagctg gaaaaaatgc tgcaggatat ccaatcatta
    18181 atcaaaaaag atacagaggt cttatgtcta ttaaatggta tcgggcatga agatattatt
    18241 gagaaatttg taccaatgga aaatatctat attggaaata ccatgtggac ggcaggtctt
    18301 gaaggtcctg gtcaggtcaa attatttgga agtggttcag tagaattaca aaatttaggt
    18361 gatggaaaag aagcagctgc aaaaaaatta gcagacaaat tgtctgaatc aggattaaat
    18421 gctcattttt ctgacaatat tcactattct atttatcgca aagcatgtgt gaacggaaca
    18481 atgaacggac tatgcacaat cttagacgtt aatatggctg agctaggaaa aacatcgact
    18541 gctcataaaa tggtggcgac gattgtcaat gagtttgcca aagtagcagc agtagagaag
    18601 attgaactag atgtcccaga agtcattgca cattgtgaat cttgttttga cccggaaaca
    18661 attgggttac attatccttc aatgtatcaa gacttgatta agaaccatcg attaacagaa
    18721 atcgattata tcaatggcgc aatttctaga aaagggaaga aatatggtgt tgcgacgcct
    18781 tattgtgatt tcttgactga gcttgtccat gcaaaagaag atagtttgaa cgtaaaataa
    18841 aggagagatt gaatggaaac ttcacaaaaa atatcaccga aaatttttct gaataaagtc
    18901 ctcgctggta cagcaacagg aatcattatt ggattgattc ctaatgcagt attaggtgcc
    18961 attttaaaat attttagtgc gtatcccttt gcggtaacga tcgcacagtt agcagttatt
    19021 tttcaactag caacaccttt gatcattggt ggtttgattg ccttacaatt tggatttgat
    19081 ccgatgagaa tgatggttgt agccggagct tcttttgttg gttctggtgt agtcaaattc
    19141 acacctgatt tgggtgaaac gggtgtttac gttggtgctg gtacaggcga tattattaat
    19201 acgatgctga cagcatcgat tgctgtcggg ctgattttac tgattggaaa tagttttggg
    19261 tcagtgtcga ttgtgttaac accgattgtt gtaggtgtag gtgcaggttt acttggtttt
    19321 tatttattcc cttttgtaac agcaattact tctgcaattg gatcattgat taataacttc
    19381 actaccttac aaccgctgtt aatgtctatc ttgattggtt gttcctttgc ctttttgatt
    19441 atctctccta tttcaacagt agcaatcgga atggcaatcc aactagatgg tgtttctgct
    19501 ggtgcggctg cgatgggcgt agctgcaacc acttttgttt tagtagtgaa ctcttggaag
    19561 gttaataagt caggtgtgac aattgcgatt gctttaggcg ctatgaagat gatgatgcct
    19621 aatctattta gaaaacctgt gattcttatt ccttgtttag ttagtgcagt gattacagct
    19681 attcctgtcg ctctattttc tatttcaggt acgcctgctt ctgctggttt cggcgtggtc
    19741 ggacttgttg gtccgttggc ttctttagat gcagggctaa atggctttct tgttgttctt
    19801 tgttggttag tcatccctgt ttttgcggca ctaattactc aatttttatg cgagaaagta
    19861 tttaaactgt atgatcgaga agaagtcttc aaatttttag gctagtagat tttactaaga
    19921 aattttcaat caatggatgc gtaagtgaga tgtcaacaac atctgactta cgtttttttg
    19981 cctttttgag taggagattg atgctgacaa ctgaactctt cagtgccatt cgatttttta
    20041 aaatgtagaa gaaactcacc tttatgttat tgattgttca aaaggcaact agaataaaaa
    20101 tatgtgttaa aatgagaaaa aatttgattg ttggatgtat cgaataatca ctaaaaagta
    20161 ggagaagaaa atgatttttt tgcaaaaata tggtttttat tttttattat taggtgtgat
    20221 tagtgattta tcgacaccgt atatccttgg ccttttttat cccaaactga atcaaatgac
    20281 taccgtaatt agtgtgtttg gtgacgtaga tagtccagtc agacgtgctt tcttggtctg
    20341 gtcagttgta tctggacttt tttttgttct ttcgttgcct gcgttgtatc acctatttat
    20401 tggaacatca aaaacacttg caatacttgc tgttgcaaca gttggtttat atgggatagg
    20461 tgattgtatt tttactgggt tattcagtat caatacgaat gaaagctctt ggaatctatc
    20521 gacttggatc cataatatag gttctgggct aggctatgca ggctttctac ttttcccatt
    20581 gttacttgtt ctgctttatc gtcaaagtgg tcaaggtacg cttagccatt tttatcttgt
    20641 tttaacagtt attagtttac taattgcagg tctttacgga ttagcacgga ttccgtcgat
    20701 tagtcagttt gccttcttta agcaattggg cttctttcaa cgcttaagtt ttttctttaa
    20761 ctatttgccg atgatttgtt taagtttttt tcaactacga aagtagaaaa agcagtgtga
    20821 agctgattaa gtaaaagaat ttctctatgg gtaaccattc gtgtggatgt gttcgactaa
    20881 gaatagagat gaggagagcg aaaagcaagt tagtcttaac ttgttatgcg ttgtgctaaa
    20941 tttcaggtag tcattttttg aatgaataag aagaaaactt gaaagagggt gacttcatgt
    21001 tagatgtgga gcagatggaa atagaccttt ataatattat tttaaatcga aatacaagag
    21061 aatgggaaag aacacaattt gaagaggcaa aagaagacct agcaaaaatg tcgataaagc
    21121 aggtgataga aaaactagag gtcaacttgc gtccgctagc actacgagat aatttgacac
    21181 cagatgcgat ggatttttat ttaagaatca caggggagtc agcgagtgca aaggcttatg
    21241 attatgccaa acataagata acttcaccct tataccaaga acgggcgatt tttgccggtg
    21301 gctgtttctg gtgtatggtt gagccttttg aaacaaaacc aggtgtaatc tcagtgttat
    21361 ctggttatac aggtggagag accaaaaaac caaattacga tcaagtcagt ggtggatata
    21421 ctgggcatgt tgaagctgtt gagatcatat ttgatacacg tcaaataacc tatgaagagc
    21481 tgttggagat ttattggcaa atttcagacc ctacagatgc ctttggtcaa tttcaagatc
    21541 gggggaaaca atatcgttca attctatttg ttgttaatga aaagcaaagg caattggctg
    21601 aaaaatcaag agaacaactg attcaatcta accgatttag ccgtcctatc gttacagaga
    21661 ttcgccaagc aaagactttt tggccagctg aaaatcatca tcaacaattt tatcagaagc
    21721 aacctaaaag atataaaaag atcaagcatt cgcatcagct tcatttattt tttcagcgac
    21781 atagatagag gaaggttgtg gcgcaaagcg ttcagctccg acaaaataat gagcaactga
    21841 caaaaatagc ttttcatatt tttggacagt tatgaattat ttcgaggagt tgctttgaga
    21901 acaccgagga aaaggttgtg gcgcaaagca ttcagcttca agtcgcaagg aaacaggagg
    21961 tatgacaagt cttttgtcac aactcctgtt tttttactag cctttatttc catgtgaaag
    22021 agaattcgaa ttgtagaatc ttgatttttc tatggaattt tgtagagaat catcttgata
    22081 attaatagac acctaatcga attccacatt aatgatgaac taaattccgt ataaaagcgc
    22141 aaacaataac tataa
//
LOCUS       DAFBQH010000036        22123 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.036, whole genome shotgun sequence.
ACCESSION   DAFBQH010000036 DAFBQH010000000
VERSION     DAFBQH010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.036"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..93
                     /locus_tag="LK694_001571"
     CDS             <1..93
                     /locus_tag="LK694_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK5052438.1"
                     /translation="NHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(164..1474)
                     /locus_tag="LK694_001572"
     CDS             complement(164..1474)
                     /locus_tag="LK694_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBK5052439.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1486..2904)
                     /locus_tag="LK694_001573"
     CDS             complement(1486..2904)
                     /locus_tag="LK694_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5052440.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3144..4712)
                     /locus_tag="LK694_001574"
     CDS             complement(3144..4712)
                     /locus_tag="LK694_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBK5052441.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4716..4997)
                     /locus_tag="LK694_001575"
     CDS             complement(4716..4997)
                     /locus_tag="LK694_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBK5052442.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4994..5917)
                     /locus_tag="LK694_001576"
     CDS             complement(4994..5917)
                     /locus_tag="LK694_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HBK5052443.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5914..6792)
                     /locus_tag="LK694_001577"
     CDS             complement(5914..6792)
                     /locus_tag="LK694_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBK5052444.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6779..8116)
                     /locus_tag="LK694_001578"
     CDS             complement(6779..8116)
                     /locus_tag="LK694_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBK5052445.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8119..9222)
                     /locus_tag="LK694_001579"
     CDS             complement(8119..9222)
                     /locus_tag="LK694_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBK5052446.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9296..10804)
                     /locus_tag="LK694_001580"
     CDS             complement(9296..10804)
                     /locus_tag="LK694_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK5052447.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10770..11462)
                     /locus_tag="LK694_001581"
     CDS             complement(10770..11462)
                     /locus_tag="LK694_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBK5052448.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11484..12458)
                     /locus_tag="LK694_001582"
     CDS             complement(11484..12458)
                     /locus_tag="LK694_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5052449.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12758..13630
                     /locus_tag="LK694_001583"
     CDS             12758..13630
                     /locus_tag="LK694_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5052450.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14206..16404)
                     /locus_tag="LK694_001584"
     CDS             complement(14206..16404)
                     /locus_tag="LK694_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5052451.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16636..17628
                     /locus_tag="LK694_001585"
     CDS             16636..17628
                     /locus_tag="LK694_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5052452.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17688..19109
                     /locus_tag="LK694_001586"
     CDS             17688..19109
                     /locus_tag="LK694_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBK5052453.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19102..20601
                     /locus_tag="LK694_001587"
     CDS             19102..20601
                     /locus_tag="LK694_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBK5052454.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20640..22034
                     /gene="uxaC"
                     /locus_tag="LK694_001588"
     CDS             20640..22034
                     /gene="uxaC"
                     /locus_tag="LK694_001588"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBK5052455.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6707 a         4509 c         3834 g         7073 t
ORIGIN      
        1 aatcatattc caagaaaatc attgaattac agaacaccaa tcgaaatatt tttgagctat
       61 gtacaagaag cattttattc tagcttaatt tgacaaatca tatatcatta aaaaaaataa
      121 aggtcaagct aaaatattat ttaagcttga ccttattata atttcaacga ttgtcaaaag
      181 tattgtttgc agctgtgttg ccagtttcta tagataaatc acagcttttg aaacataagt
      241 tttgtactgc ttcgattcgc ccgaattctt ttgcagttat ttggcaatga gaaaagcaga
      301 catcttctat aggcttttca ggaaatgcta ctaaatcaaa agctcgggaa ggaagtgagt
      361 aacctggaga aaggcatgcc tgtacgtttt tgatgtgtat gtctttgact ttcgtcattt
      421 gctttgattc aggtatttct tcagccactg cttgccaact ttctggcatt tgagcaaggt
      481 cattgctatt ttttctattg tattcattat gccagtccat tatccaagaa aaaggaaatt
      541 gtacatcaat catattcaga ttttctaccc tgatattttc gatcactccc cctctttctt
      601 ttgaagattt cattcgaata ccacagtctg actgaaaaaa attgatatcg tgaatatata
      661 cgtcactaac tccagcacta acttcgctac caatagttac accgtatcct gaacggattt
      721 tacatctagc aacctcgatt cttgatgatt ttcgattgac tcttgcacca ttgccatctc
      781 tgccagactt aatagcaata caatcatcac cacaggccaa atcacattca tagacacgca
      841 catttgttga agaatcaata tcaatcccat ctgtacttgg tccatcattg ttgcggatga
      901 ttactttttc tactaataca tcgttagaat aagtgatttg caagttccaa aaacctgatt
      961 tctgaaaaat caaatcacga acaacaacat gttctgcatg atacagtaaa caagcgcgtg
     1021 gacgtttaat agcataatcc gcaatccaac gaagtccctt ttgatcgtat tcagctcttg
     1081 tcccgctttt ttgatctttt ccccaataca gctcccacca atggtctcct tgaccgtcga
     1141 ttattccttt tccttctata agaatatctt tcgccgagag aacattcaaa atcgctgctg
     1201 gccatttcat ttctacgccg gctactcgtg tatcgatttc tggaaatgct tcgatatctg
     1261 ttctcccctt gaggactgca ccttcttcaa agataagatg actcttatct tttaaaaaaa
     1321 gggctccgac taaataaatg ccttttggaa taaatacagg taagtgttct ttcgcacctt
     1381 gatcgattgc tttttgcagt attattgtat catcttgttg agtgtcgttt gttttaaagt
     1441 tcaaagcatt gataatccct ttttttgtcc gcaatacgtt tctccctatc tttcattgat
     1501 ttttttcaat tcttcaccaa taaatacagt ttttgaacga ttcagtgggt aacctactag
     1561 caaaatgatt gcggatacaa ataagcagaa tgctggtaac aagtttgcca tcgtataaat
     1621 cctttcttgt actaaaactg attggatggc tccacctgtt gtagacgatt ggtaaccaat
     1681 gagtgccagc ataaatccgc ctaccgctcc tgcacatgct tgtcctactt ttctcgcaaa
     1741 agaatttact ccatatactg ttccatcttc tcttaagcct gtcttatatt gatgataatc
     1801 aatcacgtca gtaatgaatg cccagaccat caaattgaat aaaccagatc ctaaagtagc
     1861 gataaataaa agaatcagat atacccatgc atttgttata tgaaggaaat acatcagcaa
     1921 gtacatacat gaagaagcaa gtaacgcaaa tacgcttgct tcctttttac caaataattt
     1981 tgtcagcttt gtagcaaatg gtgctaaaac tactactgtt ccgtaagtaa ataaaagagc
     2041 aacagacatt gcttgtttat tatggaagta atcattgaat aaataagtca tattcgtccc
     2101 agctaaaatc tgattgatca caatgaaaat gtcaacaatg actaaaataa tcagtgctcg
     2161 attagctaac aaacctttta ccaactgaga agcagatact ttttcgtttt tgacaactct
     2221 tactctttca gtagtcaatt tacaagttaa catatatgcg acaaatgcta ttgcagcaca
     2281 tccgattgaa atccaaaaga aatgattccc tgaaacgact tgttgtccac tcttgttatg
     2341 ggtatacatt aaaatcggaa tgaagaatcc tgtagttgat ccgccaatcg cagaaccaat
     2401 gcttcggaac gtggacaacg aggtgcggtc attaggattt gagctaattg ctgctgccat
     2461 tgctccatac gggatattga ttgttgagag aaaaatccca taaacaatgt atgaaacaaa
     2521 aatataagca atcttcaccg tcattggaag atgttggacg aatggcaaaa acaaaatgac
     2581 agtcactaag caaaatggat acttcatttt tctgatccaa ggaataaatc ggccagtttc
     2641 tgttaactct gagacatcac ataatcttcc aaccatcaca tcagcaaacg catccaaaat
     2701 tctcgaaatc aagaataacg tacctacgat tgcccctgaa acgccaagaa cattcgtata
     2761 ataaatcatc aagaaactat tgactaaacc taaaataaaa cagttaccta gatcaccgaa
     2821 catgtagcct atcttatcgg aaatcccaaa ttcctttttt tcttttttta ctgtatttgc
     2881 tgtaacagca ttttgtgaaa gcatttgaat tcctcctacc tcttctagta aacgttttca
     2941 aaattataac tctttttttc ttgtttttat attcacaatg ttggcttttt attgcattat
     3001 cttgacagat aaaaaacaaa aaataatcta aaaacattga tgcataagat tgataacttt
     3061 ttttacataa caatatatac aaacaaaaaa attcactaac tagtgaaaat aagttagtga
     3121 acctcattta ttaaagaatt aaattacaga tgatcttttc catcatactt gaaaaactca
     3181 aaatcagcat aacttcctct ttttttattt agatcatgca ctgctatccc gataaaattt
     3241 ccagtaaacc ctcctgagag tatacacatg tttttttgcg gacctacttt cttccattct
     3301 tcagttgata cgtctttcaa atgaaactgt gctgtctctt tctttacagt gactttcaaa
     3361 aaagtcttct tttctgtgag tggaatcgtc caatcttcta tttgtgctac tccgttttct
     3421 gattctatca tacgtaggca ttttcccttg tcctcattcc aagtaacata acaataaata
     3481 taattggatt catttaagta cagtaccagt ccagccatct gattaaaatt attaggcatg
     3541 tattcaaatg ctgtctccgc atcaaaagta aaatccgttt gccgaatagc taacaaatga
     3601 tgttcgaagg tagattgaag agattcaccg gaaatcagac gtaaaaaacc tggtctacat
     3661 tttgtatcac accaatcagc atttggcaaa atgcgtctgg cattccagtt cttctttagc
     3721 tctccttcaa atgtatccat aaactgatga tctttttctt gttgtacctc ttcatttgtc
     3781 tcaatcatta tttcttccat cggagttgtc tggctatctt taagtcttaa ccaatcgtcg
     3841 gacgtccaat acacttcttg aatagcggtt tctcttccca aaatagccgc accgtttaat
     3901 ggtctggtac aaagatatgt catgtaccat cttcctgacg gggtttgtac taagctccca
     3961 tgtcctgtac attgcaatgt agaagcaggt ttgtcactag ctgtcagcat aggaaaacca
     4021 ggatcaagct cgtaaggacc agtaatattc tttgctcggc aaactgtaac tgaatgtcct
     4081 cttcctgtac cgccttctgc cgtcatcaaa taataatacc cgttacgaaa atacaaatgt
     4141 ggtgcctccg ttttcgctaa ttctgtacct gcaaatattt tatagatggg accatcaagc
     4201 ttcttcttct ctgcatcata ttcttgtaat acgataccgc tggatttatt cggtgtgtcc
     4261 agacgataat cccatagttc attgacaagc catttttttc ccgtttcaag atcatggaac
     4321 aaagaaggat caaagccgtt actatttaaa taaattggtt cggaccaagg acctgtgatc
     4381 tcagatgcag tgatcaaata attgtggaca tctttaaacg gacggcctgc acttttgaca
     4441 tctgtgtaaa caagataaaa caatccatca gcataactga tctgtggcgc ccatatactt
     4501 ccatttgtag gatttccttg caaagatact tgagtggtca aaatatccgt tgtatgtgac
     4561 cagttgacta aatttttcga ctcatatact cggattcctg gaagccattc aaaagaggag
     4621 acaataatgt aataccgctc tttcactctt aatatattag gatctggatt gaatcctctt
     4681 aaaataggat ttttcacttt gatagtctgc atgattcagg ctccttttct cattcttact
     4741 cgatataaac tgaatgcccc agctgctaaa aaagcaagtg aaataaacaa cacaacggca
     4801 aacagaccag tcatgtcctg taataatcca aatgtatttt ctaaagacaa agttgaatga
     4861 tctacagcaa gcgactgata acttttttta aactctgacg cagacatatt acttaaagta
     4921 actgctaacc cgccaaagaa aatcaaagat agactgccac cgaccattcc tagcaatagt
     4981 tcccattttc ttttcatttc tgaaccccct gcttttctaa atgtatccat tcaggtcttt
     5041 tgatcgatgt ttcataattg ttattacgtt cagagaattt gacagtctgt tcatttttct
     5101 tgtcatccca attatcccaa ccagttgggt gaacatgccc acctatctta cacgcttcaa
     5161 ataagacatt tgcatacgga cgccacggtc ttcctaaata gacgtttcta gtatcaggtt
     5221 ctgcagtcaa ggtacagctt tcaaaacgat accctgtttt ttgatttttc ggcgtagatg
     5281 ctgctgtgat gtatccagga tttttactgg ctaagctatg gatatgacac tgatgaaaaa
     5341 cggcatctgc cccaccaaat atgaaatcca ccgttccttc aatccagcaa tattcatagg
     5401 tctgacgaca atactcagga ttggcagaag ccggtgtaca aaaaggagta ccatcttttt
     5461 gcaaatcagg caacggaccc gtgcataagg tatcctgata accagatagt cgacagtttt
     5521 ttaaagatac gcgatctcct gaattgaata aagcaacagc ctgtcctacc ttttctcctg
     5581 gacctgctgt attttcgatg tgaagatttt ctaatacgac attcgatcct tctaagaaaa
     5641 aagtagcact tcggaaagtc cctaactggc taccgtcaag atactcctgt tttgcaaaac
     5701 gattaccgga gataactact gttcctgctc ccacaagttt aaaattggat aatctgcttt
     5761 ctatatattc atcatatctt ccttctttta ctatcaagac ctttggatgg ctatccggca
     5821 ggcctgccaa ataatcgatt ccttcttgga tagtttcaaa atccgtcggg gtatctatag
     5881 cattctttcc aattatcagt ttgtctttca tcctcataaa tgctccctta tgaattgttt
     5941 ccaaatattt ctcatttctt gtgtttccat atgaccgcct gttaaagaaa aagaagaaaa
     6001 gttttctgga ctacccattg attgatactt tttgtccagc ccttttctca gcttcagtac
     6061 gccttcgtcc aagcacatcg tatcgttttt tccaactaag ctaagacgag ggcgcggagc
     6121 gattttttct tggattgcta acgttgtata agctgtcaat agatttggca catagtaata
     6181 aaacccatga tgatctaggc ggcgatgtag caaaagtgtt tcgatgtcca cctgtgccgc
     6241 tagatcaata caaactttga cacgagaatc caatgctgtc acccaccagc ttaataagcc
     6301 tcccatagaa agaccaatcg ttgcgagccg ttgagtatct gtgtcttccc ttgtctccaa
     6361 ataatcgatc aagctgatca catcataaat acgcattccc cataacgttt ttcctgttaa
     6421 taaaaattgt ttgaacaact cgctttcaga aatacctccg cgttcttcaa atccccaagc
     6481 atcaatcgac caaacagcat atcccattcc tgtcagtact tctgcaaaag atggagaagc
     6541 taagtaagga gcaccatgga tcaattcact tttcccgact gaaaaatctc cgccgtgaga
     6601 atgcaggtaa aggaccgtcg gaaacggtcc ttctttttct ggataagcat agagggctgg
     6661 tactgtttcc aattcattta agtacaattg atagtattca agcataaaac cgttttgttt
     6721 ttctcggtat aatcgttttc cactaggggt atgtctgcta gatggagcac ctaaaaaatc
     6781 attcaactgc ataagcaaag tttttacgaa caaccagttc atccatattt tctgattgat
     6841 ttgattggaa tttctcaata ctgatttctt tagtattttc taatagaaat gctggtccct
     6901 cgtgattttc aatcgacatc tggttaaagg aaaccttacc cgcaaagcca atataaaatc
     6961 cttgtctgct catttcctct attcctgtca tcatagcagg aacaccggga actgcatctt
     7021 gggccatcga gacatcgata ttgtcaaatg tgatttcttg tatgaattgt tccgctaatc
     7081 cataaataaa acctgcagaa gcatggacat tccttgccgt gatattcgaa aaatggatcc
     7141 ttctgaatgc tgatgtccgt tcatctatcg gataagcctt tttttcccat acatacggtt
     7201 ctttccctcg cggaccacag aaataataaa gattcagtat aaatggacac atcacattat
     7261 ccatgactat attggatact cgaatatctt caacgatccc gcctcgacct cgtctagatt
     7321 tcaatcgtat tcctcgatca gtttcttgga agatacaatt tgaaatggta atattacgaa
     7381 tactaccact catttcactt cctaatacga caccgccatg tccatggacc atcgtgcaat
     7441 ttgtgatcgt gatattttcg caagcgatcc tttcgtacgt atcttcagtc cctgccttga
     7501 ttgcaataca gtcatctccc acgtcaatat ggcaattact aatacgcaca tttttgcatg
     7561 attctggatc aattccgtct gtatttggtg aatccgctgg gttcaagatc gtcaagttat
     7621 caaacgttgc attactgcaa agaatcgggt tgatcgtcca acttggtgac tggatcaatt
     7681 taatatcttt cacagtaatt cgatgacagt tatggaaact catcaattta ggacgaggat
     7741 aagcgagatt atctggttca tttctaaaag tatgccacca tttttttcca tttccatcta
     7801 atgtaccgaa accagtgaca gagatgttct ctacattttg agcatagata caggaagcat
     7861 aaacttttcg atggactcct tcccaacgtg aatgaacaac aggataatct tttggatcgt
     7921 cagagaattt cagaaccgct cctgcgctta aatgaagttc gacattgctt tttaaaaata
     7981 aagctcctgt tagaaattca cctgctggaa caactacggt cccgcctccg tcacttgctg
     8041 cagcatcaat ggcttgttgt attgcttcag tattcaattc atcaattgat gcgccaaatg
     8101 tcaaaatatc atacatattt ataatgcctc cacttcaatc aatgcttgca agaatgcacc
     8161 aacgcctttt tgatcattac aaataattgg ttcactgatg tagtaagcat atgaaccatc
     8221 tcgctgatct gctccgccta accctgctac ttgacagttt ttattcaagt tcagccagcc
     8281 ttcttttgtt tccagaacaa attcatcaag taatccttga tgtgatttct tcacttgtgg
     8341 aagccattct tctttgttca gcagtcccaa acgtattcct ttagccattg cgtaaacaaa
     8401 catactactg ccggaagctt ccagataatt tcctttccgt tttgtttctg ttgttacttg
     8461 ataccatgtc ccgctgtttt cttcttgata tggaagtaat gcagccagtg tcttttctag
     8521 aatttctttc agcaaagaac aatctgtttc ttcatgaagt aattcatagg tatctgctgc
     8581 agccatcaaa taccagccaa tcgatcggct ccaaaaatga tgggatagac ctgtttttgg
     8641 atcagcccat ggttggacct tcttttcatc ccatgcatga atcagcaaat ttgtttttgg
     8701 atcgaataga tgtttataac tgatttcaaa ctgtctcaaa acatcttggt aatctttttc
     8761 ttcactaaaa ttctggataa attcagcata aaaaggagaa cccatatata agccatccaa
     8821 ccaaatttga taaggataaa tttctttatg ccaaaaagct ccttcggatg tgcgtggatg
     8881 gtttgccaac tgctgggaaa ggagttctgc tgctttttta tatttttctt cgcctgtttc
     8941 tttgtacaat aaaaagaaaa gctttcctgt atttacatgg tcgatattgt attcttcaac
     9001 gctgtagcca cggatcgttc catcttcttg cacgaaatag tcgatattgt cttttatgaa
     9061 atcaaaataa attttttctt gggttcgttc ccaaagtaat tggaaccctt taagtacgac
     9121 gccatagtca taagaccatt ttcctttata ccacttttct tcatataagc gaggtgttct
     9181 ttccatgata ctttttgcta atttctttga ccagttttcc attatttgct ctcctttaaa
     9241 aattaaaata ggatttggga caaataccaa gaagtatttg tcccaatcgt ctatattact
     9301 gtgccgctgc tttgtatgct tcctcgtact cttgtgtgat tcgatcgcct ccttgtgctt
     9361 tccagtcttc tacagctttt ttgaatccat cttcgtcaag ctgtcccatg atgtattgca
     9421 ccgtagcatc aatgacgatt ttttcaattt ctgatcctac ttcattgttc gtcggtgaat
     9481 ccaatggcac agaaggatcc attacagcaa attcttcatt ttctttgatc aattgattag
     9541 agaattcttt ttcagggtct gtatctttga aagagaacgt cacttcacta ggacgagagc
     9601 ttgataacgg ttgcacctct tgttgccaaa gatccatatc gataaattcg atagaaccat
     9661 catcattcaa ttcataatgc gttccttcga taccgccagt catcaattta tacacatctt
     9721 cttctaaaag atcatcaaca aattgaagta atttcttcaa ttgtgcttcg tcttttactt
     9781 gagatttggg aaatgctagc aagccgccaa taccatttcc ttctgaccaa acatgataac
     9841 cgccgccttc accatctgag atcttgttta caggcactaa ttccatatca tcttgaatac
     9901 cttgacccat cgtcttcaga ttcttgatat ccaccattcc agtgtagatt ccagttcttc
     9961 cttgtgcaaa ttgttcttgc tgatcagtct tttgggtaac tgcaaagtct tgtgccaaat
    10021 agccattctc aaatagatct cttgaaaaat tcaaagcttt catatactct tctgtatcaa
    10081 attctggagt gaatttgcca ttatcgtcta cagcccagcc atttggtgct ccaaaataag
    10141 aagtgaaaag tttaaagctg gtataacgaa tatcactagg cgcacgatcg ccaaaaccaa
    10201 ctgtgtcgtc cacaccattt ccatcaggat cgttttctgt aaattgttta gctacttcat
    10261 aaagctcatc catggtagtt ggaacatcta gtcctagatt atccaaccag tcttttcgaa
    10321 tgactaagcc agcacgagcg tagtcttttt gtatcggtac gccgtacaag gcaccgtcga
    10381 tactcgcagc ttcgattcta tcttcagaaa tttgacttaa atttttatag tcttttaaat
    10441 attctgatac atcccagaat acgcctgatt ttaacgcatt gcggacagaa gagtttttca
    10501 acatcgcaat ttgaagtgta acgatatctc ctagttcatc agaagccaat gcagttgtta
    10561 atctttcttc ttttgaagca tcaggaatcc agttgaatgt cagttccgta tcggtttttt
    10621 cttcgatcaa gttcacaatc gtgtctgtag gcggtgaagc atgatgcaaa atatttaacc
    10681 aagtgatttt agcttttcca tcttctgtct ctgaagaact gttacttgaa ctgctgccgc
    10741 atgcggcaag tgctagaatt cctgcaaaac taagacctaa ttttacgagc gttgcttttt
    10801 tcataaattt tctccttcac tgttttaata atatttttgt tttcttctgt tttttctgta
    10861 tatgagttct tccattccaa atggcaaaat atagaatttg ctgtccacgt gactgcggaa
    10921 aagaagagac tgccaccgat aaaggttaaa acacctggca ttgtttggat gatcatcaga
    10981 taaatcccta ccatacttaa tatcaacact aatgagtaat gaagatgtga gaatccgaat
    11041 aaaaatgcca ttttcatttt gaaaaaatat cctttccaat cataatgagc catgattggg
    11101 aaaatataga gtgctgagag cacatatatg aacgcagcaa tatacaaagc gatcctaaga
    11161 taccaattgt gtatgaaaga aagatccaca agtaaaacaa tcccgatggc gccatatatc
    11221 cagctcatca gcaaggtttc acgatagttt tctttaaaat atgtgaagta cgttttcgtc
    11281 aaaggaaacg tttcttttga gcgcagccat tttctaatca ctgcaaccat cgcatacgat
    11341 gcaggaccga aagtaaaaac tcccaaacct gcgatgacta aaccgcccca cagaaaattc
    11401 aaataaacca actgtaaaat acgatttagc cttccgttta ttttatctaa tgactgcagc
    11461 atatctttcc ctctctttct atcttagtaa acgccgtctt ctcctagctt tttggcaatc
    11521 ttattagcta aaattaccat aaacaatcct acgacccctt taaacaatcc cattgctgta
    11581 ctgaaactta actgtccatt cttcaatcca gctgtataaa tataggtgtc gaaaatttct
    11641 gcaacgcttc gattcaatga attcaataac aagtacatat gctcgaaccc taagtctaat
    11701 gtctgaccga tttttagaat cagcaaggta atgattactg gacgaattgc cggcaatgta
    11761 acatgccatg ttttgcgtaa gcgtcctgct ccatccattt ctgcagcttc atacaactgt
    11821 gtatcgactg cagttatcgc tgaaaggtag atgatcgtag accagcccaa ctctttccag
    11881 atcacttgtc cgatatatac tgtgcgtgtc cattcagggg atgttagaaa actgattttt
    11941 tcaaatccca ttgttgcaag gaattcattc aatacccctc cgtcaacatt caaaaataca
    12001 taagtaatcg aaacaatgat tacccaagac atgaagtgag ggatgtaaat gatcgtttga
    12061 atcccgtttt tcaatcgttt gttcgttact tcatttaata gtaaagacaa aatgattggc
    12121 atcggaaaaa acacgaagat atttaatccg aacaagatca acgtatttct taataatgta
    12181 aagaatgtcg gttctgtaaa caaacgaatg aaatgtttga aaccgaccca aggacttccc
    12241 attattccta aaaatggttg ataatcttga aaagcaataa cgagtccccc cataggaaga
    12301 tacctaaaaa tcaagaaata aaggactccc ggcagaatca tcacatataa aaatttattc
    12361 gttttgattc ctgcaattat tttttttcgt cttctgagct tttcttcttt tttcgatagc
    12421 gtcagttgaa atggttcttg tttctttaca ctttccataa gcccctcctt agttttgtga
    12481 atgtttgctg cttatgactt catgatagat aactatctga attacgtata taatcatttt
    12541 tatcgtaagt aaaaacgctt acatagcaat gatttttgtg ctatacaaaa cgataaagtg
    12601 tattttttca caaataaact acgcatagca aaccttctat ttttttctat ctgacactta
    12661 gcataggaaa tgattatata cgtaatcttt caccgatgat aaattgaaca tatagaaaca
    12721 gaaacattta atttattttg aggaggagcc tatctatatg aaaaagaaag tctctatagg
    12781 agaaagaagt tttacacttt tcaacaatac gtttctaatt ttgctagctt tgatctgtat
    12841 cgttcctttt ttaaatatca tcgctacatc ttttgcttct acacaagaag tcgtcgcgaa
    12901 aaagtttatc ctttttccta ctaccttttc tttagacgcg tatcgctaca ttctttctac
    12961 gccgaccatt tttagagcac ttgctgtttc gattggtgtt acaggtgtcg gaacgatcgt
    13021 cagtatgtgt gcaacttcac tgatggccta cggtctttcc agaaagtatt tgtttggtcg
    13081 aggtttcgtc aactttcttg ttgtattttc tatgttgttc agcgggggga tgattccaac
    13141 gttcttagtt gttcgttctc tagggctggt caattcttac tgggccatga tcctgcctgt
    13201 ggctgttaat gcaatgaata tgatcattat gagaaacttt ttccaagcct tgccggatag
    13261 tttggaagaa tcagcaaaaa tggatggttg cacagatttt ggtgtatttt tcaaaattat
    13321 gctgccatta gctttaccgt ctattgctac tatctcgctt ttttatgctg ttacttactg
    13381 gaacacttat atgactgcta ttttgtatat caacgattca tctaaatggc cgatacagat
    13441 tcttttacgc caaatcgtca ttgtatcaag tgggatgcaa gcagaaagta gtgccgttga
    13501 tgtgattcca ccagcacaaa cagttaaaat ggctgttatc gttattgcaa cagtgcctat
    13561 gctgattgtt tatccttttg ttcagaaata ttttgtcaaa ggagcactcg taggttctgt
    13621 caaagggtga ttatgtactg aacgcaaaat ggtagttgtg tcagaagtgg tcagcttcaa
    13681 gaaataagaa ggcatttacg aaaattgctt cttaaatttt tgtgaaattt cggcttattt
    13741 ttcgaagaat ctactcctgg aacaccgtgt atcagaggtt gtgaaagaga cgttcagcct
    13801 tgagcgatga taaaaactct cacaaacatt gcgacgagta acccacagga gcaataagaa
    13861 gcgattttga aaaatagctt cccatatttc acaaaattat gccttattgt cgatagcctg
    13921 tctcttgaac gccatttatt cgtaaaaaag aactgtgaca agacttttgt cacagttctt
    13981 tttgcgtgct tagccttgaa atttaactat ccataaatac tagggttatt ctaacttctt
    14041 ttattgcttg aatcagtcca caagaaaaac agcctcttga ttaaacggcg tttatcttgt
    14101 tgattctgcg gcaataaaat cagccagcag caaaacatga agtgctgttt tttgctgtcc
    14161 tcttttattg cttgaatcag atcacaagaa aaacagcctc ttgattcaca aatatttttt
    14221 tcgatattct cccggcgtaa tcgattcctt ctttttaaag aaacgaataa aattttgcgg
    14281 atttcgatat tgcaatcttt ctgcaatctc ttttacggag aggttagtct ctttcaacca
    14341 tttttttgct acctctaaac gatgattttg aacaaaatca ccaaattttt ctcctgtttc
    14401 ctttttaaat acattactta aatagttagg gttataatgc aacctatcag cgatcacatc
    14461 taaagacaga tcacgatcat attctgtgta gacgatatgc ataatattct ccgacaaatt
    14521 tttaaattca tgatcagtga ttgcttgaga gcattctgtg attggcagaa ctaattgttc
    14581 taaaacaagt ttttctagtt ctttgggatg ataattgttc agtgctttta cataaatttg
    14641 tttcatgtta tcaaagtttt ttgaatctac tcccaataac tgtcctaatt gaacgagttc
    14701 attgatcaag cggaccgaaa ctacttcccg actcaaagga tttttatttt gtgcaaacaa
    14761 tgcatcaatc aattcatgta ctaaattctt cacttcctga tttccagaac gaatggcttc
    14821 aaacaatcga ttttgttctt ctatcggata tctaataagt gttttttcat gagcagcagg
    14881 aacgatttcc tgataaaaaa taattgaact tggaccagta tttacacgat aatacagggc
    14941 ttcttttgcc cgatcaaccg attgcttgct ttcattcagc gtttcgaacc ggttactgat
    15001 gcctatactg atagttaaat tcaagtattc ttttacacgt ttttgaatga gtcggcaata
    15061 ctccatgact tttttgcttg cttctgcatc atctttatcc attcgataaa tagtggcttg
    15121 catttcttca ttcaaaacga tcggcagcat tcgattttcc tttggcacaa tttccgtgat
    15181 catgttgttg atcgctaaaa gtagcaaatc acgctcccca ccgtgactat cttctaacag
    15241 atcgatttga accaaagctg tataatagca gtcattttga gaactataac caaattgctg
    15301 caaccgctga tctagttctc gcctttctac tcgatttcta aataaactaa gtacaaatag
    15361 cgtttccagc tgtggctttt gggtaactaa gtgtgcactt aacgcttctt tttctcctat
    15421 gatccgactg actgattgag cgaccaaatc tagttctttc ccactgaatc cttttgattt
    15481 aagattcaga cgttcctgta tttgagaaat cggacgggca aatctgtccg aaaataagta
    15541 ggaaaaaaga ccaatgagtc ctaccagtgt taaagacact gcaactagcc cgattctcag
    15601 attgcggatc attgcgccaa ttgcactaga gtcaacttct aatctgtaaa tccaattatt
    15661 ataatctgat ttcagtacta tatacgtttt atctttttct cttatcactt tcagctgatt
    15721 gtaatctaga tccacgtccg ttaatacagt tggttgctct ttatttttta ctgtctgagt
    15781 agtaaaaagg ttgccaactt cgttttcaat agaaagtatg tcttctccac tttcaccaac
    15841 aacttgttct attgtatgtt tctctatcgt agcaattccc aatgcatatt tttctttttc
    15901 aaacattggc aaagaaatca ccatttcgat tcctttgttt ttcttttgcc aaaataaatt
    15961 atttttgtta tttatgtact tctgttgata cttttcaact tcctcttcgg tcagttgact
    16021 caacgatcca ttgattactc cccatttacc agccaaactg actaaatcat aattattatc
    16081 atccataaca atcgtttcta taaaatttag ttctttcctg atttcagaat aagtggagta
    16141 atctgtatat gtcagcggat gcttgaatat gtccgagaaa tttttggtcg aaccatacgt
    16201 agaaaacgaa tattcaatcg ttctaatttt ccattcaacg ttagttttaa cttgcctgag
    16261 caaattcttt ttgtctgtta aataactcga ttcgatcgat tcataggtat tctggtaaat
    16321 gaataaacta aatcctatga caagtacggc tcctaataaa aacatcggta aaaatatttt
    16381 ataaaatact gtacttttct tcattttctt cctctcctcc ttttaaaata aaaggctttc
    16441 atttttctgt aaaattgata ctcatctaat taaatgagca ttccccgctt tcaagaatag
    16501 cataattaaa aataaaaatc caagtaattt ttaacgtgtg caatcttttc atatcaaaga
    16561 tctattgact tatcgtagtt atcgtaatat gatgaatgcg ttaacaaaaa gtgaaaaagt
    16621 aaggaaaatg atggaatggt aacgatcaaa gatatcgcta aagcagctgg cgtttctcat
    16681 acgacagttt ctcgtgcttt gaataacagc agtttaataa aaaatgaaac aaaacaaaaa
    16741 atacgacaac tagctaagga aatgaattat tcgccaaact atagtgcaaa aggtttagtg
    16801 aatcaaaaaa aatatctaat cggactattt ttctcaagtt tgcataaagg aacttcatca
    16861 aatttcctag cagattccat tgcaggcatc cattcagtat tagacacaaa ttacagttta
    16921 tctgttgaaa gtatcgatgt tgttgattta acagaaatca acctgcaacg ctacgatgga
    16981 ctgatcgtta tgagtcaaag tgatgatgat cagcctttca tcgactatct caaagaaaaa
    17041 gaatttcctt tcgttgttgt caatcgtcac atcaatgatc cagcggttat caacgtcact
    17101 gccgatgatg cggccggtgt tatgcaggca gtagattacg gtattggatg cggacatacg
    17161 aagatcgctt atattggcgg aaagaaaaat tttcgttcga caaaagaacg ggaaaaaggg
    17221 gtattgaaaa gtctaaaaaa agcgagcatc cctgtcaatc tagcctattt tttaacagga
    17281 gattacagct tagaaagcgg cctccaaaac atggaatacc tattatcact ttctgacctg
    17341 cctactctgg ttttttgcgg caacgacgac atagcgattg gtgcattgag agctgtacat
    17401 ttagctggcc tccgtgttcc ccagaacatt tctctgatcg ggttcgatga ttctccagtc
    17461 gtctcctatc tcaatccgcc gcttaccacc gtacgtaagc ctttgaaaga aatgtgtcaa
    17521 gaaggaacaa agcttttact agatttgatc gaaggcagga cgatcgaaca atctaaaatt
    17581 gagctctcca ccttgttgat ccttcgtgag tcgatcaatt acttgtaaac tacaagtaat
    17641 tttatataaa aatgttaacg tgtgcataac acagaaagga agtttatatg aaaaaactga
    17701 caaaagaatt agtgaaaaaa aacgaagcac ctgtaaaagt tcttcagttt ggtgaaggca
    17761 attttatgcg tggatttatt gattggcaga ttcagcagct gaataaccaa caattgttta
    17821 aaggtaatgt tgcaatcatt caacctttag aacaaggtct tggaaatatg atgaaccagc
    17881 aagataattt atatacggtc attttacaag gactcagcaa tggcgaaatc gttgatactt
    17941 ccgagattat caccgtagta gaacaaatga tcaatcctta tgatgaatgg gatgcttatc
    18001 tatctctagc agaaaatgac gacttggcct atatcgtctc caatacaaca gaggcaggaa
    18061 tccaattcgt ggagactgat aatttaacag atactccccc acagagcttc ccgggcaaac
    18121 tgacagcctt tttataccga cgtttcaaat taaacaaagc tggctttacc atcattcctt
    18181 gcgaactgat tgacagaaac ggcgaacgtc taaaagaaat cattttacgc tacgcagaaa
    18241 aatgggattt agaacctgct tttgttgagt ggttagaaaa agaaaatgtt ttctgttgta
    18301 gtttagttga tcgcatcgtc ccaggttatc ctcgcgaaga tgcaggtgtt ttcaatcaac
    18361 ggcataacta tgaagatcaa ttgatggtca aagcagaacc attcatgctt tgggtgatcg
    18421 aagggcctaa agaattggag aatcaactac cattcaaaca ggctggcttg aatgtcatca
    18481 tcacagatga tatgactcct tatcgtgaac gtaaggtgca tttgcttaat ggaccgcata
    18541 cagcgatggt ccctcttgct ttgttggcgg aattggaaac tgtagaagaa gtaatgaaag
    18601 atcctttatt ctccgcatat gttgatcagt tattcaatct agaattgatt cctatgcttt
    18661 ctttacctaa agatgaatta gccatttatg ctgaccaaat caaagaacgt ttcttgaatc
    18721 cttttgccca tcacaaatta gaagcgattt cattaaatag tgtttcaaag ttcagcaccc
    18781 gtctgcttcc agtgttcaag aagtatatcg aagaacaaaa tcaggtacca ccgttaatca
    18841 ctgtttcact agctgcgcta cttcttatgt atcgaggcga tcaagtaaag ccacatgatg
    18901 atgaaaagac gataagcgaa tttactgatg cttggtctga taatggaaca gctattcctc
    18961 gattattgca aaacgctgcg ctttggggag aagatttgtc tcaaataccg aatgtgacag
    19021 atacagttca agaaatagct gcaaagatcg atcaaaaagg tgtccggtca gtattacaag
    19081 aaatgaaagg aacgatggtc catgcttgaa accctgcaac caacaatgaa attaaatcga
    19141 cgagacagtg tggtcgtcgc attaacccct attccaaaac aaacacaact gacgattgat
    19201 cagcaaacga tcaccactct tgaagatatc ccgcaaggtc ataagattgc attgggtgat
    19261 ttgaaagctg gagacaacgt gatcaaatat ggctatccaa tcggtcatgt gacgacagac
    19321 gtaactgccg gtcaatggct gcacactcat aatgtaaaaa ccaatctttc tggtgaattg
    19381 gattatcgat atgaaaaaga tgtccatcca agtcattacc tttttgagaa ccgaacattt
    19441 caaggttact tgcgaaaaaa tggaaaagta gggattcgta atgatttgtt tttagtacct
    19501 accgttgggt gtgtgaatgg cattgctgaa ctgatcgtca aacagttcaa ggaaaaacat
    19561 ccagatctgg gacagtttga ccatattacg attttaaagc atccttatgg ctgttcgcag
    19621 cttggaaaag atcatcaaaa tactcgagaa attttagcgg acgcagtaaa tcatcccaat
    19681 gccggaggag ttttagtatt tggattaggt tgtgagaaca atacagttcc tgaattcaaa
    19741 aaaattttag gtgaatatga tgaagaacga gtgaaatttc tagtggctca agatgtttac
    19801 gatgagatcg aacaaggtgt ggctctatta gaagagcttt taacagcggc ggaaaatgac
    19861 cagcgcatat cagtcccgct ctctaagttg aatgtcggtt tgaaatgcgg cggttctgat
    19921 ggtctatcgg gaataactgc taatccttta cttggtgcat tttctgacta cttgattgct
    19981 caaggaggca gcacgatctt aacagaagtg ccagaaatgt ttggcgcaga acaagtattg
    20041 atggcacgag cggaaaatga agaagttttt gactcaatcg ttgacttgat caatgacttc
    20101 aaacagtatt tcttgtctta cggggaacca gtttacgaaa acccatctcc tggtaataaa
    20161 gcaggtggga tcactacatt agaagataag tctttaggct gcacgcaaaa atcaggaagc
    20221 tctcctgtcg ttgatgtgct acaatatggc gaaaaaatca gaaaaccagg gcttagttta
    20281 ttgcaagctc ccgggaatga tcttgtagct gcctctgctc ttgcttcatc cgattgccag
    20341 ttggtcttgt ttaccacagg aagagggact ccgtttggct catatgtccc aacggtcaag
    20401 gtatctacga atacaactct ttttgaacgc aaaggtcatt ggatggattt taatgcaggc
    20461 gtcttattag aacaaccaat ggaagtaatc ttagaatcgt ttgttcaaaa aatcatcgct
    20521 gtagctagtg gagaagaaac gaaaaatgaa caaaatgacg ttcgagaaat tgccattttt
    20581 aaaaacggag taacactttg attttcataa aacagtataa aaagaaagaa ggaagattta
    20641 tgtttttatc tgatgatttt ttattaaaag acgattgggc gaaaaagctt taccattcct
    20701 acgcaaaaaa catgcccatt attgattatc actgtcactt agatccaaaa gaaatttacg
    20761 aaaacaacaa tttcagcaat ctgacagaag cttggttagg aggcgatcat tacaaatggc
    20821 gcttgatgcg agcttgcggc gtgcctgaag aaaaaatcac aggaaatgct tctgattttg
    20881 aaaaattttt agcttggtgt caaaccgtac caaaaattat cggaaatccg ctttacagtt
    20941 ggtcacatct tgaattgaag cgtttcttcg gtattgactt gttgataaat gaagaaaatg
    21001 cagagaaaat ttggacacaa gcaaatgaaa ttttggcagc tcctgatttt cgccgtcggg
    21061 aaatcgcaaa aaaatcgaat gtagaagtga tttgtacaac agatgatccc gcagacgact
    21121 tgcactatca tcagttgctt gctcatgaag aaccagattt gaaggttcta ccttcattcc
    21181 gtccagataa agcactcaat attgaaaaag aaggatttaa tgcatgggtc acactactgg
    21241 aagaagcagc tgaccaaacg atcaccacct atcaagaact gatagaagtc ttagggcagc
    21301 ggatagaata cttccatcaa catggatgtc gtgtttccga ccacgcttta gacatcttac
    21361 gctataaaga agcggatgaa acagtattag aagagatttt tcaaaaagca cgagctaaca
    21421 aactattatc ttcagatgaa atcgatgctt atcttacaga aacactgata catttgaccc
    21481 acttctatca ttctcataat tggacgatgc agctgcacat ccatgcttat cgtaactgta
    21541 atacacaaat gttggaaaaa ttagggcccg ataccggcta tgatggaatg aatgatcttt
    21601 ctttgactat tcctctgcaa aaattattga accgtgcaga agaaacggat cagttgccaa
    21661 agacgatcct ttattcattg aatccgaatg actatcctgc gattttagct ttgatgggct
    21721 gtttccaaaa agaacagaat ggaaaactgc agttaggttc tggatggtgg tacaacgata
    21781 ctcgagctgg tatgcgcgat caattgacac aatttgctga tggtagtgct ttggggaatt
    21841 ttgtcggcat gctgacagat tcaagaagtt tcctgtccta cactagacat gaatatttcc
    21901 gtcgtgttct ttgtgaattt ttaggcgaaa tggtcatgcg aggagaagca cctgaagatg
    21961 aacaactttt aggaagtcta gttcaagcga ttagttatac aaatgctaaa aattactttg
    22021 gcttttttgc ctaattgatt aatagtaaca gtaacgaatc aaaaacagga tagtgaaata
    22081 acggaatccg tttatccacg ttttagagaa gaagacggac caa
//
LOCUS       DAFBQH010000037        21797 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.037, whole genome shotgun sequence.
ACCESSION   DAFBQH010000037 DAFBQH010000000
VERSION     DAFBQH010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.037"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            127..3654
                     /locus_tag="LK694_001589"
     CDS             127..3654
                     /locus_tag="LK694_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBK5052456.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="LK694_001590"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="LK694_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBK5052457.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="LK694_001591"
     CDS             7442..7783
                     /locus_tag="LK694_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5052458.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="LK694_001592"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="LK694_001592"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBK5052459.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="LK694_001593"
     CDS             9140..11560
                     /locus_tag="LK694_001593"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBK5052460.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKEINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="LK694_001594"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="LK694_001594"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBK5052461.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="LK694_001595"
     CDS             12994..13731
                     /locus_tag="LK694_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK5052462.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="LK694_001596"
     CDS             13745..14560
                     /locus_tag="LK694_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBK5052463.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="LK694_001597"
     CDS             14573..15220
                     /locus_tag="LK694_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK5052464.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="LK694_001598"
     CDS             15236..15892
                     /locus_tag="LK694_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK5052465.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="LK694_001599"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="LK694_001599"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBK5052466.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="LK694_001600"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="LK694_001600"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBK5052467.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="LK694_001601"
     CDS             18234..18752
                     /locus_tag="LK694_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBK5052468.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /locus_tag="LK694_001602"
     CDS             18859..19359
                     /locus_tag="LK694_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK5052469.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19639..20559)
                     /locus_tag="LK694_001603"
     CDS             complement(19639..20559)
                     /locus_tag="LK694_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBK5052470.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20724..21737
                     /locus_tag="LK694_001604"
     CDS             20724..21737
                     /locus_tag="LK694_001604"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBK5052471.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
BASE COUNT         7312 a         3771 c         4660 g         6054 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aagaaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
    19501 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt
    19561 ttagagcgtg ggacaaaagt gttctttact tttgtcccac gctcttttta gtgagattcg
    19621 ttgcatcgtt tgtccaaatt attttccgac agcaattgga ctgttcggaa tcgtgaaacc
    19681 agaagctgtc aattctttga cataggagct caagaactct tcttttaagg catattgttt
    19741 tccgttcaat gcgtagcaga ccgtacgaat cgcaaaattg ctgtttccaa gatccactaa
    19801 tccaaagata gaaggttcag tttggagctc ttcttggtat ttttcagcta atgtacgatt
    19861 tactcgatca atgatctcat agattttgtc atatccttct tcaggaacaa tccgaatatc
    19921 taaaagtact tgcatatttg cacgagagag attgctgatc gtggtgatgt ttcgattcgg
    19981 gatgaaatgg acagtaccat ccgttgcttt cagctgaagg gtacggatac caacagatac
    20041 gacggttcct tcaatcgtta agtttgctag cttgatgtag tccccaacat ccatctgttg
    20101 ttccatgata ataaaaaatc cggtaataac atcactcatg aacccttggg caccaagacc
    20161 gattgccaca cctgcaattc cggcaccagc caaaagtgat ccgatcggca caccgatagc
    20221 agataaaatt gcatagataa aaaagaaacc aagggtataa tgaaaaatag tatttaaaag
    20281 actatgtaaa gtctttaagc ggctctcgtt tagaatggct tttttagaat actgattaaa
    20341 agacttatca atcagatatt ttcccacacg gtgaagaatc caaaaaagga cgatcaaaaa
    20401 aatcagatat aaactttttt ggataaaggc agccaaaatg gcgtcccaat caatcccgtc
    20461 ccaaaaccgc tgaaacgcat ttactttgct gacagcttga tctgtcaatg tctcgccaga
    20521 agagtctgtc gtaccggcag ttgctaataa tgaaaacata aaatcctctt ttctaaaatt
    20581 tgattctttc tgtattgtac caagaagttt tcaaaaaagg aaatagcaaa aataactata
    20641 atgaaggaaa aaagctgaaa ataaaatgac aaataatatg gataatgata aaatgtggag
    20701 aaaataacga aggtggtaag tatatgcaaa tcgattggaa caatctaggt ttttcttata
    20761 tcaaaactcc ttggcgtttt attgccaaat ggaaagacgg acaatgggaa gaaggagaat
    20821 tgacagaaga taattacctg acgattcatg aaggctctcc agcgcttcat tatggtcagc
    20881 aatgttttga aggtctaaaa gcttatcgtc gaaaagacgg caaggtcaat ctctttcgac
    20941 ctgaacaaaa tagtcgacgg atgaatcaaa gtgctagacg attgctgatg ccaatggtcc
    21001 ctgaaaagat gttcgttgat gctgtcaagc aagtggtaaa agcaaatgaa gattttgtgc
    21061 cgccctatgg aacaggcgct acattatact tgcgcccttt actgattggg gtcggtgaaa
    21121 acattggtgt ccatccagct ccagaatatt tatttgtcgt cttctgtacg ccagtaggag
    21181 cgtatttcaa aggtggacta aaaccaacga attttattgt atctgattac gatcgtgctg
    21241 ctcctaaggg gactggtgca gcaaaagtcg gcggaaatta cgcagcgagt ttacttccag
    21301 gaacagaagc aaaagaactg gattactcag attgtgttta tcttgatcca gccacacata
    21361 cgaaaatcga agaagtaggt gcagcaaatt tctttggaat tacaaaagac ggaaaattca
    21421 tcactccgca atcgccctct atcttaccaa gtatcacgaa atattccttg ttgcagttag
    21481 ctgaagaacg actaggactg caagcaattg aaggagatat tttcattgat gatctagatc
    21541 aatttgcaga agctggtgca tgtggtacag cggctgttat ttctccaatc ggtgggattt
    21601 atcacaataa caatcttcat gtattttata gtgaaacgga agtaggacca gtaacaaaac
    21661 ggttgtatga tgaattgaca ggtattcaat ttggagacat agaagcacca gaaggctgga
    21721 ttgaagtcgt ggaataattt caaagtaatg agttgaaaaa aacgtaaaaa gaaaaaatca
    21781 ggaagagact ttaaaaa
//
LOCUS       DAFBQH010000038        21664 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.038, whole genome shotgun sequence.
ACCESSION   DAFBQH010000038 DAFBQH010000000
VERSION     DAFBQH010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.038"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(412..1908)
                     /gene="lysS"
                     /locus_tag="LK694_001605"
     CDS             complement(412..1908)
                     /gene="lysS"
                     /locus_tag="LK694_001605"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBK5052472.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2019..3029)
                     /gene="dusB"
                     /locus_tag="LK694_001606"
     CDS             complement(2019..3029)
                     /gene="dusB"
                     /locus_tag="LK694_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBK5052473.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVE
                     NALAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKW
                     MLDYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADL
                     KGEKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQ
                     TL"
     gene            complement(3030..3917)
                     /gene="hslO"
                     /locus_tag="LK694_001607"
     CDS             complement(3030..3917)
                     /gene="hslO"
                     /locus_tag="LK694_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBK5052474.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4134..6245)
                     /gene="ftsH"
                     /locus_tag="LK694_001608"
     CDS             complement(4134..6245)
                     /gene="ftsH"
                     /locus_tag="LK694_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBK5052475.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6335..6880)
                     /gene="hpt"
                     /locus_tag="LK694_001609"
     CDS             complement(6335..6880)
                     /gene="hpt"
                     /locus_tag="LK694_001609"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBK5052476.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6880..8325)
                     /gene="tilS"
                     /locus_tag="LK694_001610"
     CDS             complement(6880..8325)
                     /gene="tilS"
                     /locus_tag="LK694_001610"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBK5052477.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8445..8915)
                     /locus_tag="LK694_001611"
     CDS             complement(8445..8915)
                     /locus_tag="LK694_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBK5052478.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8961..9386)
                     /locus_tag="LK694_001612"
     CDS             complement(8961..9386)
                     /locus_tag="LK694_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBK5052479.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9453..9722)
                     /locus_tag="LK694_001613"
     CDS             complement(9453..9722)
                     /locus_tag="LK694_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBK5052480.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9733..11328)
                     /locus_tag="LK694_001614"
     CDS             complement(9733..11328)
                     /locus_tag="LK694_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK5052481.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11343..14864)
                     /gene="mfd"
                     /locus_tag="LK694_001615"
     CDS             complement(11343..14864)
                     /gene="mfd"
                     /locus_tag="LK694_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBK5052482.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14881..15441)
                     /locus_tag="LK694_001616"
     CDS             complement(14881..15441)
                     /locus_tag="LK694_001616"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBK5052483.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15794..16768
                     /locus_tag="LK694_001617"
     CDS             15794..16768
                     /locus_tag="LK694_001617"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBK5052484.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16938..18338
                     /locus_tag="LK694_001618"
     CDS             16938..18338
                     /locus_tag="LK694_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBK5052485.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18416..19138)
                     /locus_tag="LK694_001619"
     CDS             complement(18416..19138)
                     /locus_tag="LK694_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5052486.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19217..19723)
                     /locus_tag="LK694_001620"
     CDS             complement(19217..19723)
                     /locus_tag="LK694_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBK5052487.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19846..21645
                     /locus_tag="LK694_001621"
     CDS             19846..21645
                     /locus_tag="LK694_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBK5052488.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WEEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         6011 a         4729 c         3885 g         7039 t
ORIGIN      
        1 gcaactcatt tatcataact tatcgtttgt tatttgtcaa gaactttttt gaagtttttt
       61 tcgatagaat caagcttcgc aagtggttct tttttaacga ctcagttatc ttatcatgtc
      121 gttagtaaca tgtcaataac ttttttgtta tgtttttgaa taaatatttt cttctgtctt
      181 ttggacaagt aataatatac caactaaatt tagcttggtc aacgaatttt ttgtattttt
      241 tcaagttttt ttcaataaac gattattcat aacttttctt tctttatcgt tcgttttcgt
      301 cttctttaat gtctagaata gtcggcatta gcttttgaac tttcgttatt atttattcct
      361 ttctacacag caaaagctgc ctaaggaatt ccttagacag cttttattcg attatctcat
      421 tgttgggaat agcaaaacat cccgaattga ttgtgcatca gttagcaaca ttactaatct
      481 gtcgatcccg atgcctagac caccagttgg cggcataccg tattctaagg cttccaagaa
      541 gtcttcgtct acaccgtgag cttcgtcatt tcctagttca cgttcttttt cttgcgcttc
      601 gaatctttct ctttgatcaa ttggatcgtt cagttctgta aaggcattag caaattcgcg
      661 ccccactatg aatagttcga atcgatctgt gaatcgagga tcttctggat ttttcttcgc
      721 taaaggcgaa acttcgactg gatgtccgta tacaaacgtc ggttgtgtca atgtttcttc
      781 aacaaatgtt tcaaagaatt cattgataac atgtccgaca cccatagctt cggttacttc
      841 tacattgtgt ttttcggcta aagctaatgc ttcttccaat gtcatttctt gccagaagtc
      901 tacaccagtt tgttctttga ttgcatctac catatggatg cgtttaaagt cactttctaa
      961 gtcgacagct tgtccatcat atgtgatttt tgctgttcct agtacttttt cagctgcgtt
     1021 acggataata ccttctgtca gattcattac atcattgaaa tcagtatatg ctgtgtatgc
     1081 ttctaacatc gtaaattctg gattatgtgt agtatcaatt ccttcattac ggaaaacgcg
     1141 gccgatttca taaacttttt ccattccgcc aacaattagt cgttttaaat gaagttctag
     1201 cgcaatacgc aaatataaat caatgtccag tgcattatga tgcgtgataa atgggcgtgc
     1261 ggcagctcca cctgcctcat tgtgcaatac tggtgtttct acttcaatat agccattgcc
     1321 atctaaatag cgacggattt cgctgataat ttggcttcgt ttcataaatc ggtcaaagct
     1381 ttcgcgatta ctgattaaat ccaagtagcg ttgtctgtag cgttgttcaa tattcgttaa
     1441 gccatggtat ttatctggca gtggacgaag tgcttttgac aaaatcgtga tttctgaagc
     1501 tttgatagaa acttcgcctg tgtctgtttt catgatttgt ccagttacac caaagaaatc
     1561 tccaaggtct gcatgtttga atacttcata ggcctcatca cctacttggt cttttcttac
     1621 ataaatttga atttggcctt cacgatcttg tagatgcgca aatccagctt tcccttttcc
     1681 tcggcgagtc atcatacgac cagctacact agcggtcaat cccatttctg ctagttcttc
     1741 ttttgttctt ggatcaaata gttcatgcaa ttcttctgag ttgtgcgtgc gttcaaatcg
     1801 ttttccaaat ggatcgattc catcttcacg caattgttcc attttttgac gacgtacaag
     1861 catttgatca tttaaatctt ctacttgatg ttgttgttcc tgtgtcacaa aagttcctcc
     1921 attctcatgt taatctcttt cctataatgc caatgaaaca caaaaaaagc aagccgaact
     1981 tggaatttcg gcttgaaaat tcacgaaaaa cttagaaatc acaacgtttg tttcaatagt
     2041 actttttccg ctttttctac aaattgatcc agcaggtcat tgatttcttg ctgtttttct
     2101 gtctgattga ttgctacttt cactttcgct gctcgaggaa tcccttttaa ataataagcc
     2161 gcatgtgttc tgaattctct acaagctact ttttctcctt tgagatctgc taagcgttgt
     2221 aaatgaagtt tagctgtagc aattttttcg gctggtgttg gttcaggaat cagttctcct
     2281 gtttcgagat aatgctgtgt gcggtgaatc atccatggat tgcccagcgc tgcacgacca
     2341 atcatcacac cgtctgcacc gacatagtca agcatccatt ttgcatcttc tggcgttttg
     2401 acatccccgt tccccataaa tgggatcgtc aaatgttttt tcacttcttt caatacttcc
     2461 cagtccgctt ttccttcata catctgaacc cgtgtacgtc catgcatagc gatggcactt
     2521 gctccagctg attgtgctgc taatgcattt tctacagcaa agacgtgctc ttcatcccaa
     2581 ccaatacgca tcttgattgt tactgggata tttactgctg aagaaacagc ttggaccatt
     2641 tcgtatactt tctcaggatc taaaagccat tttgctcctg ctcctgcttc tgctttgatt
     2701 actttattta ctggacagcc catattgata tctattatat cggcagctgt gttttcttcg
     2761 acaaattgag cagcttcaac tagactatct ttatttccgc caaatatctg taaacttaat
     2821 ggatgttctg tttcttcaat gtggagcatc tctaaagttt ttttgtttct gaaatgaata
     2881 ccttggtcac tgatcatttc acatacgact aagcccgcac caaattcttt gacagtgact
     2941 cgaaatgctg aatttgttat tccagccatc ggtgcaacaa cgacacgatt agggatttcc
     3001 acattgccaa ttttccatgt gtgttccact taaatccctc ctaaggcttc tttcttcaat
     3061 ccatctaatt cttcttcgct atacttgtat ttgttattac agaaagaaca aaccgcttct
     3121 gcaccgtgat cttcgtctat cattgcttgg atttcttctg cacctaacgc gataattgct
     3181 tctgcaaatt tttcttttga acagtcacat ttgaattgaa caggcatttt ttccaaaatg
     3241 ttcaattctt ctgtaccaag caatcctttc aatatttctt ctggtgtctg tccttcatct
     3301 aagagggtag agattcttgg tgtcgttttt aattgttctt cgatcttgct gattgtttct
     3361 tcatcggcac ctggcatgat ttggatcatg aatcctcccg ccgtcttgat gctgtcgtca
     3421 gtattgacca atacactcag tccaacagct gaagggattt gttctgagat agcaagatag
     3481 taagtaaaat cttcagcgat ttctccagaa acaatcggcg tttgcccaga aaaaggttct
     3541 tttaatccta agtcttttgt gacagtaaac attccttgtg ttccaactgc tccccgcaca
     3601 tcgattttac cttcactatt tagaggaaga ctgagatgcg gatttttgat gtaacctttc
     3661 acatctccct ttccatttgc atctactaca attcctgttg ctggaccatc gccttggatt
     3721 cgaacagtta gtttgtcttc tcctttgagc gtcgcaccaa gtagcagact accgaccaat
     3781 gtccgtccca aagcagctga ggaggtgtgc caagtatcat gacgtctttg tgcctccgct
     3841 acggtatttg ttgcttgaac ggcgtatgct cggacgaatc cgtcgtgagc aagtgcctta
     3901 accaaataat ctttcatttg ttatgtactc ctttttccgt ttttaaactc atagtgaatc
     3961 atactagcaa ctactgcttt tttcaacttt ggaatgcttc ttttaaaaat aattcagaat
     4021 catctagaaa acaggacatc accctattta caacttaaat aaagaaatct cccgaaaacc
     4081 ggtttttgtt ccggttttcg ggagtcatat cagatcaatt gacctatatt tttttatttg
     4141 tattgatcgt ctgaatgatt atcttctgat tgttgagaat ctgaatcatg ttctgcttta
     4201 ttttcctcat tcagttcatg ttttgcttct tcaaaatcac gtttttcttc cgcttgtttt
     4261 tcagcgtctt tttcttccaa agcacgtttt gcttcttcaa aagttttcgc ttctttttcg
     4321 cttggataat catttgactc agtaccttca ggcatcacac cgtgttcaaa caatgacttg
     4381 attgctttgg catccaatgt ttcgaattcc aaaagttttt cagcaatcag tttatgctgt
     4441 gcacgatgag cttcgatgat ttctcgagct ttatcatgag cttccatcaa gatttttcgt
     4501 acttcttgat caatctcgaa agcgacttgt tccgagtaag cttttgtttg accatagtcg
     4561 cggccaacaa acacttgatg gtttccttca tattgaacag gacctaaacg atcactcatt
     4621 ccatattctg ttaccatgct tcgtgctaga gctgtcgctt gctcaaagtc attggaagca
     4681 ccagttgatt ggacattgaa gatgatttct tcagctgtac gtccaccaag taagcctacg
     4741 atttgttcaa acatatcttc tttcgtcatc aagaactgat cttcttttgg taaagcgatc
     4801 atatatccgc cggcgcgtcc gcgagggatg atcgttactt tgtggacgac ccgagcgcga
     4861 ctcaatacta atccgacaat cgtatgacca gcttcgtgat aagcaaccat ttcgcgttct
     4921 tttttgctga ttacgcgatc ttttttagct ggacctgcaa tcacgcgatc ttccgcttca
     4981 tcgatatcag aagcatcgat tttcttttta tttcttctag cagctactaa cgctgcttca
     5041 ttcaagacat tttctaaatc tgctccggca aaacctggtg tctgttgagc cacaactttc
     5101 aagtcaacgt catctgccat cggtttattc cgtgcatgga ctcgcaagat tgcttcacga
     5161 ccttttacat ctggacgtcc aactaaaatc tggcggtcaa aacggcctgg acgtagtaac
     5221 gctgggtcta gtacatcaga acggttggtt gcagcaatga cgattactcc ttcatttccg
     5281 tcgaagccat ccatttctac aagcaattgg ttcaatgttt gttcgcgttc atcatgtccg
     5341 ccgcccatac cggcaccacg ttgacgacct accgcatcaa tttcatcaat gaaaatgata
     5401 gctggtgcgt ttttcttcgc tgtttcaaac aaatcacgaa cacgacttgc tccaacaccg
     5461 acaaacattt ctacgaaatc agaacccgag atcgagtaaa acggtactcc agcttctccg
     5521 gcaacagctt ttgctaataa ggttttacct gttcctggag gtccttctaa aagtacacct
     5581 gccgggatac gggcacctaa ttcaacaaat cggcgaggat ctttcagaaa ttcaacaact
     5641 tcaactagtt cttgtttttc ttcttccgca cccgctacat cagaaaaacg tacgcgatta
     5701 gcttttttgt ctgcttcttt ggcttttgat ttaccaaagt tcatgacacg gccgccaccg
     5761 ccaccgccgc cgccttgttg tcccatcatc atgtaaaaga aaaagatgat gattacgatt
     5821 ggcaagaagc taagcaatag ggaaacccat ataccgctgc ttgattcttc ttttactgtt
     5881 gtagcaactt tgttttcttt tgccagttct gttacttggc tcaatgttgt atcatttggc
     5941 aaaatgattg ttgtgaaatg ggtagtagtc gtatctgttg aacctaagat agctaaccca
     6001 ccgctgttag cgacggtctg ctgttctttg tattcaccag tgatcttata tacaccattt
     6061 gcaggctgca cttcaaattc tttgatcttg cctgcttgta gttgttctgt gaattttgag
     6121 tactcgatat ctgacgatcg ctggccgtta ttaccaaaaa tgaaataaac gaccattacc
     6181 atcgctaaga ttacgaggac ataatacaag gcatttttca tcatgccgtt gttctttttg
     6241 ttcatagttg ccctccctaa gcgtcaattt aatttaacag ttttttatca atcctgacca
     6301 ttctattgta ccataattta attgagatac agcattaatt tgattgataa acactaggtt
     6361 tcaatacacc gatgtacgga aggttacgat atgcttctgc ataatccaag ccataaccga
     6421 ccacaaattc atttggtacg ttgaagccaa cataatctgc ttcgatattt actactcttc
     6481 cttcaggttt gtcaagtagt gtaacaattt tgactgattt tgctttgcgg tatttgaaaa
     6541 gatccactaa ataagcaagt gttcttccgc tgtcaatgat atcttcaacg atcaacagat
     6601 cgcgaccttc tacattcgta tctaagtctt tgacgatttt tacttcccct gacgagactg
     6661 ttgcattccc gtagcttgaa acatccatga aatccaattc caaatacgta tcgatagaac
     6721 gaacgatatc tgccatgaat ggtaccgctc cttttaagac acctactact aggggattgg
     6781 tgttttggta aatttctgtt aactctttgc ctagttcttt gcaacgaact tgtatctctt
     6841 cttgtgagat caaaattctc tcaatatcct tttccaacat caggttctcc ttccaattgc
     6901 ttctttttga tatttgtaaa gaagtatgta gtgtatttta tcagtttctt cacgaatact
     6961 caaatatgaa aaggcaaaag gtatcaagct atatatattt cttttggcat ctgtaatgat
     7021 ccaagcatgc tgcctttgtg aattaggtac tttctgatcg ataaaatatc tagatacttt
     7081 ttttgtgaga ttctctgtta atttaatgcg gtctcctgct tttctttttt cgacatacag
     7141 ttgcacatca gcaggaatcc aaatgtcatg agaaaataga gaccatgaat ctgttgcttt
     7201 gatgtcccct gcattttcag gtttgaccag accaatccat tctgtttcat tcaaatatat
     7261 tccttgttct ggaatcagca acaagcgctg gtcttctgtc tggctagttg cttgtttttc
     7321 ttttttagag agtacaacaa aatgatactc tcttttcagt atccaattat tgccaatact
     7381 gagttgccat tggctttttt tctgttgcca ctggtctaaa atcgactcca tttgttgttc
     7441 tttcaaaata attcctgttt ttgagaatgc tttgtcgaaa aaggagtaaa aaaaatagta
     7501 tcgttcagca tctgtcattt cttcaatcat ttctgcagaa aatcgaaatt gttcctccca
     7561 ctgttcgact tccttttcga tcaactgtcc catatatttt tgaatgactt ggtctgccca
     7621 ttggatctgt tgcgaaaatt gctggaaatg tctcaacgcc tgtgtatttt cttgtttcaa
     7681 ttgaggaact accaaatgac gaagccgatt tctctggata tctaacaatt ggtttgtctg
     7741 atcctcaaag taaacaagct gcgattccgc tgcataagta tataggtctt ctttgctaaa
     7801 actcagcaac ggacgaatca gtctgcccgt agcaaatggt tgtacttctt tgattccaga
     7861 aaaagtattt aactgtcccc cacgaacgat tttcattaac accgtctcga tttgatcatc
     7921 tccgtgatga gcagtcatca acgtgtcata ttgtccttct tccatcacgc gagtgaattc
     7981 ttgataacga aagttacgag cttctgtttc tatggcggat ttggctggat cttcccaaat
     8041 acttacatat aaagggagat tcttctcttc acaatatgag cgaagatagg cagcttcttc
     8101 tgctgattct tctcttaatt ggtgattgat gtggacgata cctaaagaaa aaccgatttg
     8161 gcgttgcact tgtaaaagta aatggagcaa aaccatcgaa tcaacaccac ctgataccgc
     8221 tgctaaaatt ctcgatcctt gttgccagaa tccttgttca ttcccgtgcc gaataaatga
     8281 ctgctgtaat agcggtgctt ttttcccgcc tttttcaacg ttcatccttt ccacaccctt
     8341 tcttcctaaa aaaaagagag cgggaaaacg tctaaaacga caattaacct gccctctgtt
     8401 gatactatga cccaaatagt cgcactatca ttatttgcgg ctttttagct gcgacgtccg
     8461 ccacggccac cgcgctttcc ttctgtgttt cgcttcaatg aagataagcg gtcatcgcta
     8521 tctttcaaaa atgaactcat caatgaatcg aagtcttctt tttgagctga aggttgactg
     8581 cgagaaaaag attttttctg tgctggacga tttcggttgt cacggggatc tttttgttga
     8641 tattttttag gacggctttc ttctccagat tcagcaggtt gttcctgcgc tttccggata
     8701 gatagtccga tttttccatc atcatttact gaaataactt taactgttac ttcatctcct
     8761 acacttaaaa catcatggat gtcttttaca tagccatttg acacttcgct aatatgaact
     8821 agacccgttt ttccagcacc tagatcaata aacgcaccaa aatttgtgat tcctgatact
     8881 tttccttgca gttttgctcc tacctcgatt gacataaaaa aaatgttcct cctaattatt
     8941 tccctttttt atattcctgc ttattttgca gttgtagaac tagttgattg atctgtttca
     9001 tttgtcgagc tgtctgtatt tgattctaat ataggataaa ctttttcacc gtcttttgag
     9061 taaaagaatt tgcttctggc aagtttagct acatactcat catctttcag caatgaaaca
     9121 tcttggtgca gctgatcaac gtctgcgttg acttctttca tttcagtatt tgcttcgact
     9181 ctcaattcgt tcaattgatg catcctttgc atgtcatgga aaatctgcac acctattatc
     9241 gcgaaaatga ccaaagcacc aacaaaaatt gcagccagtc tgcgtcttcg aaaaatcagt
     9301 tgacgttgtt gttgctgaaa ttcagcatac tttttctttg catattccgt atctagcgca
     9361 gcgattttat tgcttttttt cgtcacttgg attcgctcca atctttttga ttgtcttttt
     9421 cattatacca atatcgtgaa agcttgtcta atctatttct ctagtgtttc tgtgcgattt
     9481 tcagaaataa tttcgtacat tttttgtgca tcttcttttt tggttgattc atgtaattcc
     9541 gtaattttca cttccaacgt tttgttgcca aactggactt tcaaaatatc gcctactttg
     9601 acatcactgg aagatttcgc caatttaccg ttgatttgaa ttcttccttt atcagctact
     9661 tcttttgcta ctgatcgtct tttgatgatt cgggaaattt ttaaaaattt atctaatcgc
     9721 atatcttatt cctcactttt taattttttt atttaatcgc aggattttct taccaaaagg
     9781 caacattagc cattctcgta ctgtaaacag acgaagacga atcactgcat aaatgaacac
     9841 agaagccccg atcccaactc ctatcacgct gacaaaaaaa gcagtggaac gatgagttac
     9901 aggaccaaat actatattca atacaccata atacagcagc agtactactg tcatacaaag
     9961 aaggctgaac atcaacttcc gtccaaaatg ccgttctttc caaaaagcat ttagctcttt
    10021 tgtttcaatt cgaacaaaat accaaagcgt cacacctaat cctaaaaggg tagacaggct
    10081 tgcacctgct gttcctaata aaccggtcag aaaagaagta gtcaatcctt ttaccaatag
    10141 tccccatcct gcgcctttta aagaagggcg aaacgagttc ttgctttgag caatactttg
    10201 ataagcttgg atgactgcag tgaaagcaat ggaaaaaaca aataatacaa gcgctgcatt
    10261 tcccgcataa tctttaaaca acgcataatt gatataaggg agcagcaagg ctaatcccaa
    10321 agacgcagct aatgctaaag cggtcgtcaa tctaagatag attttggctg tctgtaaaaa
    10381 ctgacgatag atccggttgg tcaaatgacg agtcaacgca ggcaggaatg tcgcacttaa
    10441 tgcagtggca atcactaaac ctaattgtac taaaggctga cctcgatcat agaccccttt
    10501 tgctatcttc gcaccgtatt ctgacagtcc gtaaacttcc aaagcatttt ttacaaagaa
    10561 agagtcgatc agctggaaaa agataagcaa tccactataa atcgaaacca gtccgccttc
    10621 taccagaaaa cgccggatca agggctttga cggctgtttg aacaatggaa accgtcggaa
    10681 gcttaacgct cctccatgaa tcttttgttc atagtagtga aggatcccat atgcgcataa
    10741 accgccaaaa acagctccac tcattgctgc cgtccctgtt tggtacaccg tccatccaag
    10801 ctttctaaaa gcaaacgcac tacagacaat cacaaaaaca cgaacgaact gctcaactac
    10861 ttgagaaaca gcagttggtt ccatcaagaa tcttccttgg aaattccctc gataaaaaga
    10921 tagaccaggc atcagcaaaa aggtaaagga aactacttgg atcagcggtt tcaattgtat
    10981 gtctcccatc atccaagcaa tccagctgct aaatgcaaag acaaacaacc acaaaaagac
    11041 acctgtccaa aatactaagg gataaagatt ttgcaaagct tgctttcgct cgaaaggatt
    11101 ctttctttct gcaacgtact ttgatatgaa ctgcggaaga ccggataaag ctaaagtcat
    11161 tgctatccca taaattggat aaacttgctg ataaacataa aaaccttcgt cgccgactaa
    11221 gttttggagg ggaattcggt aaagggcact taatactttt gctatgaaag aagcaatcgt
    11281 caatacaaat gctccttgca tgacgcgccg catttcttta tgatccaaat cattgctcct
    11341 tcctacgctg acagatattt ttgctcacgc aacgctttga caaacttttg gatttcctga
    11401 agccatactg cttcttccat ttggttgggg atcgtcaagc gtatcaccat ctgttctttt
    11461 tcaacgccta gactggcttt tagttttgtc gctgtcagtg cttcgaataa ctgttccaca
    11521 ctatagcgtt ttgttccaac tttacttaga gtaaagacta tatgcgcctc ctgtttcctt
    11581 atcgtttcta ggagcgctcg atcgccatcc attttgatct gcccaattgt caagagatgt
    11641 gctacttctt ctggatattc tccaaaacgg tcgagcagat catcttctag ttcatctagc
    11701 atttcttgcg aatcaagttc acgaattcgt ttatagatct cgattttctg ccgttgatct
    11761 gccacgtatg tttcaggtag atacgcatcg acaccgaggt cgatctcaac agtggtcttc
    11821 tccacttgac tattctttcc ttgtttacga gaaacagctt cgcttagcat ttgagaatac
    11881 atatcgaaac cgactgcatc aataaatcca tgctgctgag cacctaagag attacctgca
    11941 ccgcgaatgg aaagatcccg catcgcaatc ttgaatccag atcccagttc tgtaaaatct
    12001 ttgattgcct gaagacgttt ttcactgact tcgttaagga tcttctgctg ctcatacata
    12061 aagtaagcat atgcgacacg gctgcttctt cctacccgcc ctcgcaattg gtataacgtg
    12121 gataatccca tataatcagc gttctcaacg aacaacgtat ttgcgttcgg gatatctact
    12181 cctgtttcaa tgatggttgt agtgacaaga acatcatatt gaccttcaat aaattcaaaa
    12241 agcgtatttt ctaattgcgc ctctgtcatt tgcccatgag cataaccgat ccgtgcttcc
    12301 ggaacgagtt cttggatttc ttctactttt tgttcgatcg tttctacccg attatataaa
    12361 tagaatactt gtccacctcg tcccatttct cgatggattg cttcgcgaat agccccagga
    12421 tttttttcca taacatacgt ttggatcgga taacgattcg caggcggtgt ttcgattact
    12481 gaaagatccc gcacgcctaa catcgacata tgcaaagttc tcggaatcgg tgttgcagtc
    12541 agagtcaaaa catccacttg tgcacgtaac tgtttcaatc gttccttatg ttttacacca
    12601 aaacgttgtt cttcgtcaat gatcagtaga cccaaatcgc taaattcaat atcttttgac
    12661 aaaattcggt gggttcctac aacaatatca acttgacctg tacgcagttg ttcgatggtt
    12721 tctttttgtt gctttcttgt tctgaaacga cttaatagcc cgatatttac ggggaaacct
    12781 tcaaatcttt ccagcatcgt ttcatagtgc tgctgtgcca agatcgttgt tggaactaaa
    12841 aaggctactt gtttgctttc ctttatcgct ttaaaggccg ctcgaagcgc tacttctgtt
    12901 ttcccataac caacatcacc tacgagtagt cgatccattg gtttttcttt ttccatatcc
    12961 cgtttgatct ccgctgcact tctcaactgg tcatcagttt cagagtaggg gaaggcattt
    13021 tcgaattctt tttggtaggc gtcatcaggt ccaaaagcat atcctttttc tgactctctt
    13081 gcggcatata atttgattag atcgtcagcg atatcctcga tttttgaaga gacttttcgc
    13141 ttggttttcg tccattcact tccgcctagc ttattgatgc gaggcgcttt ggcttcagag
    13201 gcgacatatt tttggatcag attcagttga gtaacaggaa taaacagttt gtcgtcgttt
    13261 tgatacaaaa tagtcatata gtcttggtga acgccatcta cttctagcgt ttccatccca
    13321 atgtatttcc cgataccatg attggcatgg acaacgtaat ctcctgcttt taactcatta
    13381 tagcttttca atcgctctgc gtttgttact gtttgacgtc tcgtttgttt tttcgtcgtt
    13441 ttttggaaaa tttctttttc tgtgatagct actatttttt cttgaggaag ttcaaaacca
    13501 gcctgcaaag ctccttcaac taattgtacc tgtcctctga tcaatgcatt tttttctgtt
    13561 tctactacta atatgtcttc atctcgaagc atttgttctg ctttgcgtat tctgtccttt
    13621 gtcggaataa agatcacaac agtctgtttt tgcttgcgcc aacggtccat ttctgttttc
    13681 agcaatggca tttgtccgaa gaactgttgc atcgtccgat attgaaaatt atggattgct
    13741 tggaaacgaa gatttcccat ccctttttgg aagagtgaga aaaaggttgt ggtgaacttt
    13801 tccttctgca cgattttttg tatatctgct gaaaatgtct gctcagaaaa cacccgcaat
    13861 tcgctgattt tttgagtctg ccattctgcc gcttcccttt cgatttcccg atttgtttcc
    13921 atgattcgct gataatcatc aacgactagt aaactatttg aagggaaata atccaagatc
    13981 gtcacagatt cttggtaaag aagatctgag tagaattttg cttcttctgt cgggacacct
    14041 gcttgccagc ttgaagtcag ctgaccgaag taatcttgca aaaactcgcg gtctgcttct
    14101 tctgtggtcg cagcaagccg tttttctagc attttttgca tttgctctat tccatgttct
    14161 agatcctctg gtccgtagac ttgttcactc gttggggaaa cccagatttc ttccgttttt
    14221 tcgattgagc gttgtgtttc tgcttcaaaa taccgcatag aatctatctc tacatcaaat
    14281 agttctacac gaacaggata ggttgtcgtt aacgggtaaa tatcgataat gcttcctcga
    14341 atactgaatt ctcccggttt tgcgaccatc tcttctcgta catagcccat caaagtcaat
    14401 tggcgaggaa gattttccac gtctatctca tcaccaatat gccaattcaa ttgcgcctgt
    14461 agccaagttt tcttattcgg cagtcgtttg tgcaaagatg cgactggcaa caaatagata
    14521 cctgcttttt tttcttgaag tgcgttaagc gttgcaactc tctcgcctcg cgcttctgga
    14581 gaagaaaagg ccatttcagc agaaagcact tcatccactg gaaataaatg gatctgatct
    14641 tgcggaatca catgttgcag atcttctgct aactgattcg cataatacaa atttggcatc
    14701 agaataatca tgtttttatt tgttttctta aacgctcgag caaatagaag tgttttcgct
    14761 gagcccgcta atcctgtgat tagttgtcta ggcatttttt cttctaaatt gctgacccat
    14821 tcttttactt gcggatcttt tccgactaat tgaataatat ccattccttt tcctccttcc
    14881 tcaattgaat cgattcatcg catccacgaa cgaatgccct tctatgagat attcgactgc
    14941 tttaacagat tgacacatgc tttcttctat caaaggctga ttttctttgc tgaaaggact
    15001 aagcacatgc tggactactg tttttttccc ttctggcctg ccgattccca ccttgattcg
    15061 atcaaagaca ttcgtattca aatgggaaat aatacttttg ataccgttat gcccgccggc
    15121 acttcccttt tgacggagcc ggatttttcc cactgccaga tccaaatcat cgtagattac
    15181 gactaattcc tctggataaa tcccaaaata agtcattagt gggccgactg cacgaccaga
    15241 ttcattcatg aatgtttgtg gtttgactaa aaggatcttt tctccattat gaaaaaattc
    15301 ggcaacttct gcttcgaacg gatttttttt gaaagtagct tgatgctcgc gtgcaatttt
    15361 atccacgacc ataaagccga tattgtgttt tgtatattgg tatttggtac ctgggtttcc
    15421 taacccgacg atcattttca tttttgtttt ccacctaatt ctaaaaagca gcacaaaagg
    15481 ctggactgct aattagcggt ccagtccatc gagctttttt cttttttata cttgtcaatg
    15541 catagtataa cacaaaacaa ggcgtttcgg ctaaaagaga atcgttaata aatgataaat
    15601 aaattaaaaa tttcttcaaa gtttaatgaa ataattgata tataaaaata gtgaaataac
    15661 gcacatgtaa gcttttacat ataatacaac cttgttatct gttatatttt atagccaaat
    15721 agcgtgacat ggggcttgag aaatgttagc ataagcatgg gaaaaaacaa atcaatggaa
    15781 ggattgatac taaatgactg cagctgcaga aaaaaaagat catcaaaaag ttattcttgt
    15841 tggagacggc gccgtaggtt ctagctatgc ctttgctcta gttactcaaa atatcgctca
    15901 agaagtaggt attgtggata tcaatactgc taaaactgaa ggagacgcaa ttgacttgtc
    15961 tcacgctttg gcatttactt cacctaaaaa aatctattct gctacttacg cagacgcaca
    16021 tgatgctgac ttagttgtta ttacagctgg cgcaccacaa aaaccaggtg aaactcgttt
    16081 agacttagta aacaaaaacc taaaaatcaa ccgcgatgtt gtcacacaaa tcgttgaatc
    16141 aggatttaac ggtatctttt tagttgccgc taacccagta gatattttga cttactcaac
    16201 ttggaaattc tcaggtttcc ctaaagaacg cgtaatcggt tcaggtacct ctcttgactc
    16261 agctcgtttc cgtcaagcac ttgcggaatt agttgacgtt gatgcacgta acgtccatgc
    16321 ttacatctta ggtgaacacg gagattcaga attcccagtt tggtcacatg caaatgttgc
    16381 cggcttgcaa atctatgaat gggtaaaaaa caacccagaa atcgatgaag aagctatggt
    16441 taacctattc tttggcgtac gtgacgctgc ttacacaatt atcgagaaaa aaggcgctac
    16501 tttctatggt atcgcagtag cattagcacg tatcactcgt gccatcttag atgatgaaaa
    16561 tgctgttctt cctttatcag tattcatgaa tggcgaatat ggattgaacg acatctacat
    16621 tggtgcacct gctgttatca accgtcaagg tatccaaaaa gtaatcgaaa ttcctttaag
    16681 tgacagcgag caagaccgta tggctgcttc tgctaagcaa ttaaaagaaa ttctagatga
    16741 agcttttgct aaactagacg ctgaataatc tttgaacatg acggaaaaca tctattcggt
    16801 gttttccgtt tttttatttt atcaccctta tggtttctaa attttttcat aaagtagaga
    16861 taaccttcgt cttttcctct aaattatgct atcatgagac cgttccaccc taaactttta
    16921 cttaaggagt tgtcgatatg acaagatctt cgcatcgaaa aaaatcgaaa catgttgctt
    16981 tgctttcagt tcttggtgtt ctcttagtgc tgatcagctt ttatacctat cgcagtacat
    17041 actacacaaa gcattttttg ccgaatacag aaataaatgg aataaatgtc agcaatctaa
    17101 cagtcgaaaa agcaaatgaa aaattaaaag aagcttattc tgataaaaaa ctggctatta
    17161 aagaagatgg aaaagtctgg caggaagtac caaaatccga gctcggctac aaagatgatt
    17221 tcacttcgga gctttctccg atcctaaatg aacaaaacgg atggacttgg ggaatgactt
    17281 atgtctcagc ggcagaaaaa caagaaatag atcctgtcag tcaaaaccac caaaaactag
    17341 acactaccgt tcagacattg acaactaagc tgactgattt gaacaaagac cggacaccta
    17401 ctactgatgc aacgatcgaa aaagcaggtg actctttcgt tatcaagcct gaagtcaatg
    17461 gaaatacgat cgacgtcgat gctgcggtca aacaattaaa aagtgcggta aactctggta
    17521 aagatacaat tgaattgact gaattcaaag aaaaaccaaa agtaacatct gaagacagct
    17581 cactgaaaga acaactcgca tccatgaatg ctatcgcaaa tgtaaaagct acttacagta
    17641 taaacggtga gactttccag atcccatcaa gtgatattat gagttggctg acctacaatg
    17701 atggaaaagt cgatttagat acagaacaag tacgtcaata tgtaacagat ttaggcacaa
    17761 agtataacac aagtacgaat gacacaaaat ttaaaagtac aaaaagagga gaagttacgg
    17821 tcccagttgg cacttacagt tggacgatcc aaacagacag tgagacagaa gcattgaaaa
    17881 aagcgattct ggcaggccaa gattttactc gttcacccat tgtgcaaggt ggcacaaccg
    17941 ctgatcatcc attgatcgaa gatacgtata tcgaagttga tctggagaac cagcatatgt
    18001 ggtattacaa agacggaaaa gtggcccttg aaacagatat cgtttccgga aagccaacta
    18061 cacctacacc tgctggagtc ttctatgttt ggaataaaga agaagatgca actttaaaag
    18121 gaacaaatga cgatggaaca ccttacgaga gtccggtaaa ctactggatg ccgattgact
    18181 ggacaggtgt tggtatccac gattcagact ggcaacctga atacggtgga gacttatgga
    18241 aaacccgcgg ttcacacggc tgcatcaaca ccccaccaag tgtaatgaag gaattattcg
    18301 ggatggtcga aaaaggaacg cctgtattgg tcttctaaca aaaataatat ataaaaagaa
    18361 agcacccgta cgattggtac aggtgctttc tttttattat ctaacaatgt gctacttaca
    18421 tttcagcagc agtaaagact tctgaaacat catcatcgtc ttctagctta tctactagac
    18481 gttccaattg ctctttttgt tcctcattta aatcaatcgt cgtttgtgga atcatcgtca
    18541 attctgcttg cgccaaagtg aactcttttt ccaactcatc tcgtacagca gcaaaatctt
    18601 ctggtgcagt ataaatctcg aatacttcag gagaagtttg cagatcttct gcccccgcct
    18661 ctaatacatc ctcaaacatc gtctcttcat caacagtcaa cccatcgcgt tcaatagcga
    18721 tatatccttt gcggtcaaac aagtaactga ctgaacccgt ttctcccagt gatccaccgt
    18781 ttcgagtaaa agctactcgg acatttgtcg ctgtccgatt acggttatct gtcaatgcat
    18841 ggactaagat tcctactcca cccggaccat atccttcata cgtgatttca tcgtaattcg
    18901 tttcatctac ggcactcgta gcctttttaa tcgcccgttc cacattgtcg ttaggcatat
    18961 ttgcagcttt tgctttgtcc acaacaagcc gcaaagctgg attcatagat ggatcaggac
    19021 cgcctgcttt agctgccata taaatttcac gagaaagttt ctgaaagact ttccctcgtt
    19081 ttgcatcttg ggcatttttt cttccttgga tattatgcca tttactatgt cctgacatga
    19141 tttttcctcc cttgttcttt tgagaataca cttctattct agtactattt cacaaaaaaa
    19201 caaagccaca gccaatctat aaacgcttct tgaatgccgc aaaaagcaca cataatatca
    19261 cagcagtcag agcaccgatc gaatccaaca tgacatcttg gaacataggt gttcgtccac
    19321 cggtcatcat ctgatggaat tcgtccaaac ctgcgtaccc tgttgctgaa agccaagcaa
    19381 ggatcgctgt cagccaccaa atttttagct tggggaaaac acctaaaaac aagctgcctc
    19441 ccaaaataaa ataggtaccg aaatgtgcgc ccttccttaa aaagaactct aaaaatttgg
    19501 cgtagcccag atggtcgata ctcaccatac ttccaccata attaaaacgt atacccgcta
    19561 atgtgtcttt aaatggctga ttaggcagcc agctttctaa tcgcgaaact tgtgattgct
    19621 gtccgtaagt ttgagaagaa ctgtaaaata agattataac gactatcagt gcgattacta
    19681 aataaatatt tccgtttttc atttgttttt ttgaagaatt cataagtcac ctctgtattt
    19741 tatcttagac tttttttgaa gaaacaacaa tcattttcat agagacttct tccataaaaa
    19801 agtgctatac ttaattttgt attacaatat gaagggagtt ttttcatgag ttattcatta
    19861 aattggaatc tagattctat tttttccgga ggcagtcatt ctgatgcttt gaaccaacgg
    19921 atgaagcaat tggaagacca aatgaacgag tattatcacc gcgtaacaaa atggtcacct
    19981 agttctgaca atgcagagca gttaaacgct attcttcaat tgcaagaaac gattacgaat
    20041 ggttttaccc aatgcagcag ctatattact gcattgcttt ctgccaatgt caatgattca
    20101 gatgcaaagg tcttatcagg caagctttac gcaatgctgc ctcgtctgca atcagccgaa
    20161 actgtattgt caaagaaatt tgcagaaata tctgacaatg attggaatca gatgctttct
    20221 caatcttcgt ttgaaacaat tgcttttcgt ttaaacgaga ttcgtcgtga tggctcacaa
    20281 ttattatcag aagctgaaga aaacattata aatacgcttt ctttagatgg tctaaatgct
    20341 tggagcagtc attatgatac gattgtagcc agcatttcga ttcccttcga acaagatgga
    20401 caagtagtcg aattatctgc tggacaagcc ttcaataaga tgatgggcga tccagatcca
    20461 aaagtcagag aaacactttt cgcagcatgg gaagaagcct gggaagaaaa ggcacccctc
    20521 ttcacggata ctttgaatca tttagatggt tttagacttt ctgactatga acttcatggt
    20581 gtcacagatt ttctacaaaa accattagag tacaatcggt tgaagaaaga aacattgtct
    20641 gtcatgtggg atacaatcca gaaaaacaaa caaccccttg ttaattactt gactagaaaa
    20701 gctaatcttt tcggtaaaga aaaaatggaa tggcaagatc aagatgcacc tattatttta
    20761 ggcgatttga aagaaaaaac ctttagtttt gacgaagcag cagcatttat tatcgaaaac
    20821 tttaacaaat tcagtccaaa aatggctaaa tttgcccaat ctgcttttga aaagagctgg
    20881 atcgaagcag aagatcgtcc tgggaaacgt cctggtggtt attgtactga gttaccagaa
    20941 acaaaagaat caaggatctt tatgacctac agtaattctg ttaatgaggt tgcgacactt
    21001 gctcatgaac taggacatgc tttccatagc agcacgatgt gggatttacc ttctttaaat
    21061 cgcgaatatg ccatgaatgt cgcagaaaca gcaagtacat tcgcagaact gatcgtagcc
    21121 gatgctacat taaaagtcgc aaaaacaaaa gaagaaaaaa tcaatttgct agatacaaaa
    21181 ctgcaaaatg ctttagctat gttcatgaat atccattccc gattcatttt tgaaaatcga
    21241 ttctatgaag cacggcaaga aggattagtc tcagaagata agatcacaga gatgatggtt
    21301 gaggcgcaaa aagaaggcta tcaaggagct ttagctactt accatccata tttctgggct
    21361 gcaaaattgc atttctttat tgatgacgta ccgttttata acttcccata cacatttggc
    21421 tacctcttta gtttgggcat ctatgcttac gccaaccaaa agggagcaaa ttttgaagaa
    21481 gaatatatcc atttactacg cgatactgcc tcaatgacga cggaagatct tgcgaagaaa
    21541 catttgaatg tcgatcttac ccaaccagat ttttggcaag caggtatcga catggtgact
    21601 gaagatatcc aaacgttcat ggaattgaca gaagaattta tctgatttct attttatcac
    21661 aata
//
LOCUS       DAFBQH010000039        21643 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.039, whole genome shotgun sequence.
ACCESSION   DAFBQH010000039 DAFBQH010000000
VERSION     DAFBQH010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.039"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            144..542
                     /locus_tag="LK694_001622"
     CDS             144..542
                     /locus_tag="LK694_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBK5052489.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1221
                     /locus_tag="LK694_001623"
     CDS             526..1221
                     /locus_tag="LK694_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBK5052490.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1805..2692)
                     /locus_tag="LK694_001624"
     CDS             complement(1805..2692)
                     /locus_tag="LK694_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBK5052491.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2877..3716
                     /locus_tag="LK694_001625"
     CDS             2877..3716
                     /locus_tag="LK694_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBK5052492.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3912..4886
                     /gene="sgrA"
                     /locus_tag="LK694_001626"
     CDS             3912..4886
                     /gene="sgrA"
                     /locus_tag="LK694_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBK5052493.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5184..5540
                     /locus_tag="LK694_001627"
     CDS             5184..5540
                     /locus_tag="LK694_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052494.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5543..6700
                     /locus_tag="LK694_001628"
     CDS             5543..6700
                     /locus_tag="LK694_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5052495.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6726..7166)
                     /locus_tag="LK694_001629"
     CDS             complement(6726..7166)
                     /locus_tag="LK694_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBK5052496.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7326..7832
                     /locus_tag="LK694_001630"
     CDS             7326..7832
                     /locus_tag="LK694_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK5052497.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            8000..9913
                     /locus_tag="LK694_001631"
     CDS             8000..9913
                     /locus_tag="LK694_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBK5052498.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9918..10919
                     /locus_tag="LK694_001632"
     CDS             9918..10919
                     /locus_tag="LK694_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK5052499.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(11121..11327)
                     /locus_tag="LK694_001633"
     CDS             complement(11121..11327)
                     /locus_tag="LK694_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBK5052500.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11973..12821)
                     /locus_tag="LK694_001634"
     CDS             complement(11973..12821)
                     /locus_tag="LK694_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK5052501.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12820..13887
                     /gene="gnd"
                     /locus_tag="LK694_001635"
     CDS             12820..13887
                     /gene="gnd"
                     /locus_tag="LK694_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBK5052502.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            13889..14890
                     /locus_tag="LK694_001636"
     CDS             13889..14890
                     /locus_tag="LK694_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBK5052503.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14887..15171
                     /locus_tag="LK694_001637"
     CDS             14887..15171
                     /locus_tag="LK694_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBK5052504.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15204..16001
                     /locus_tag="LK694_001638"
     CDS             15204..16001
                     /locus_tag="LK694_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBK5052505.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            16015..16848
                     /locus_tag="LK694_001639"
     CDS             16015..16848
                     /locus_tag="LK694_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5052506.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16866..17723
                     /locus_tag="LK694_001640"
     CDS             16866..17723
                     /locus_tag="LK694_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK5052507.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17745..18254
                     /locus_tag="LK694_001641"
     CDS             17745..18254
                     /locus_tag="LK694_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5052508.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18291..18692
                     /locus_tag="LK694_001642"
     CDS             18291..18692
                     /locus_tag="LK694_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052509.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18804..19157
                     /locus_tag="LK694_001643"
     CDS             18804..19157
                     /locus_tag="LK694_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052510.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19191..19634
                     /locus_tag="LK694_001644"
     CDS             19191..19634
                     /locus_tag="LK694_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBK5052511.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19651..20736
                     /gene="uxuA"
                     /locus_tag="LK694_001645"
     CDS             19651..20736
                     /gene="uxuA"
                     /locus_tag="LK694_001645"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBK5052512.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20729..21442
                     /locus_tag="LK694_001646"
     CDS             20729..21442
                     /locus_tag="LK694_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK5052513.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         7457 a         3323 c         4509 g         6354 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcatgcgata
     1081 acgttaggta aagagctact agatcaagta ttagagatgc cctcgattga taaagcagtg
     1141 aggggcttcc aagaagattg ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa
     1201 aaggtcgctg attacttctg aatcaatttc tccataaaat aaacgatagc cttctcgaaa
     1261 actcgaggag gctatctgac gattcaatat tcattaattt aaagtttggg tgactattac
     1321 ttatttctca tttaaacgat gtattcccta acctctaata catccttgta actctgaagt
     1381 tacgccagtt ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata aaaatatctt
     1441 tcgtgtttct ctagtataac agaataagag gaacaagtcc agaagggata tttttagtct
     1501 tttttaaaat aacttttcag tcgagtttga gtttatgata gttgtttttg aagcgatacg
     1561 gcaagtgaaa aaatagggaa gcatgaaaga atctacttaa tggcgcattg attcatgtga
     1621 tagaaataaa aaactttttt aaattatatc ctcatgattg taacaaatcc agtgatttgt
     1681 tttggtacaa ataaaaaaag gccccgatga agttgtggca aaagtggttt gcctcaaaaa
     1741 taagaaggaa tttccaaaaa tcacttctca aattttgcca catccccaat tgggtcaaat
     1801 ttgtttattt gaatatattt tttacataag gatctttaat agttacattg tcttttttca
     1861 tcactttacg gattgtttta tccatgaatt ccgtatcaag agtttttgct tcggtagcaa
     1921 ttttttctag ctcagatttg tatttgtctt tattagaacc tttatcctgt ttttttacca
     1981 tttttactag ataatagctt tgctgataag tcgttggatc tgttacagga atgatatcag
     2041 aaacttcccc gttttttagt ttaaatgctg ctttttttac ttcagtagga atctctggat
     2101 tggttgaatc aaaattcatt tttccatcag tttcctttaa tgttttattt tttccataag
     2161 cttgaacaag cttggagaaa ttttcccctt catttgcttc tttttttgct ttatctgctt
     2221 cttctttagt cgaaaataga atgatctgtg tactaacttc tggatagaat tctttccaag
     2281 cagtgtctag atctttatct gttaatttta cattggcttt caaaccgtat tggaaagcca
     2341 gcatctgttt aaagagtttc ttatatgtct cttctgtttg gccaactgct tttaattgtt
     2401 ccttgtagtt tggaccgagt tttttgattt gttcattata ttcttttgtt acttcctttg
     2461 aagaaatctc ttttccgtat acatcatcaa atgctttgaa gacaatcatt ttttttaata
     2521 gtgtttgatt atttgattta acttttgggt tatcataaaa atcctgaact gtaagtgttc
     2581 cacctttaaa cgttgcaatc gtttcattgg acttgctaca acctgagaat agtaaaattg
     2641 cacatgctga aaggcacata attagtagtt gttttttcaa aaaatttccc atcctttcct
     2701 tattaaaaac agtctaacag aagaattagg gagacagtct ttcattttcg taaggttgct
     2761 gtagatattc ttagttcgta aaaatctatt ctctctatga tggagaacat ttacatagtt
     2821 ttcacgagac aagaaagaaa agggttgtta aacttaatat aaaggaggga tgaaagatga
     2881 tcaggattat tcggttgatt ggatctgttt taatagtctt agttggtttt gttttaggaa
     2941 ttcttgtcaa taatgcactt gcggatatgt ttattggaaa tagtgaatgg attggagcaa
     3001 ttgccggtac ggtcggacat ttagctgttt ttttactagc tttatttctt gctagtaagg
     3061 tcgaaggaaa aagaatagaa gattatggtg tttctgttcg aggaaaagac tgggggtatt
     3121 tagcgggagg tctcgttgtt ggaattggag tgtttctgct tattactagt ccattatatt
     3181 tgacgggggc gtatcggtta gattcaggaa acgcaaacat tgtgccgctg ataacgagtt
     3241 ttattttatt tattgcagtg ggggcatcag aagagctttt attccgtggc ttttttcagc
     3301 atcagatgct tagatttggt ccgttaatag caatgattgg atctgctgca ttatttgctc
     3361 ttttgcacgg attgaatcct aacatgacat ttctggcagt tttcaatatc tttttagcag
     3421 gttgcttttt cagcgcactt atctacagta caggaagttt atttacggct attggtgctc
     3481 atattacttg gaattggaca caaggagcta ttttgggtat accagtcagc ggaacgcaag
     3541 aacaaggttt tttctccact ctgatccaat cgcaaaatac aataattact gggggaaact
     3601 ttggggctga agcatctata tcaactacat tattcttgtt agtcttgatt gttggtttgt
     3661 tactctatgc atataaaaca aagcggatga agaaatacac tgctgtttat caataaaaga
     3721 tgaagaaagc aaggctgata tcggtcttgc ttttttaagt ctgtcattga ttaaacgaaa
     3781 aagtaaacaa attcaagcga atacacgaca tcttttgtta gattttcaca actgactgtc
     3841 gtgttctcat cattttcatt ttatataatt cctttatcaa atagaaggta attatatgga
     3901 gagtgataga aatgaagaaa acagcgaccg tattatttgt tattcttggg ctgagtataa
     3961 ttgcagcaat gattggaatc agtacttctg caaatgaacg ggcaaatgag aatctttcat
     4021 ttactgtgaa aactgatcgg attgtttatg acatgacaca acaagtcatt actattccag
     4081 tcaaaccaaa caaaagcgtg aatgctagtg atgtacatgc tgttctaaca tatggttggg
     4141 atggtaatgg aagttcagaa aaagttattg gggaagttta tttaaaagac gtacaatgga
     4201 ccgctggtat tgagtataca ataatgattt ctgcagaact atcgattgat gagatcaaga
     4261 gtaaagataa agttgatttg attgtttttt atgatggaca gatgacgatc acggagaatt
     4321 tgaaaccaag cagttggaca gttgttggac cgccaagcag tacggatagc acaagtagtg
     4381 aaagcagtac agaaaacacg agcggtgaaa gcagtacaga gagtacgagc agtgaaagta
     4441 gcacagaaaa cacgagcagc gaaagcagta cggagagtac gagtagtgaa agtagcacag
     4501 gaagcacgag cagcgaaagc agtacggaga gtacgagtag tgaaagtagc acaggaagca
     4561 tgagtagtga gagtagcaca ggaagcacga gcagcgaaag cagcacagga aaagtaaata
     4621 atgaacttga tttggtacct atcataccaa ctaaggaaca aaagagaaat gcagggagca
     4681 acctaaaaag tgtgctaaaa cagcctgatt ttgtttctcg tggaattgat catcaagaaa
     4741 aagcgaaaga aacagataat agaaaaagcg atttaccaga aactggtagt agaaccttga
     4801 acaagctaat tacatggatg ggtgtgttac tcattttgat tgtaggagca agctattttc
     4861 gttctctctt ccatcgagtg aaataaagaa taaaagcaac agatctaaaa ttgaataaaa
     4921 cgatagattt ttgagtgaat atttcattcg agagtgctgt attgcctggc gaagttgtag
     4981 ctttgccaga tctatttttg tttactaaca tatttgagaa acaggaaata acaggaacac
     5041 aaatggaaaa taagagcaag aataattgac aatgcacact gcgcatggta gtatctctga
     5101 aaaatcagta acgaatctat ttgtacgtta ttcatgtagc tgtcggaata agtctaaata
     5161 atagataaaa aggagtatgt actttgtttg aagaatttat tgatatcaat gaaaggcaag
     5221 tatatcaatt tttgaactat tgttatgaaa gagacgagaa attatatgtg gtgaaggata
     5281 tcgctttaga tctaaactac accctggcta agatgaattc ggtgattcaa caagcagaat
     5341 cgttttgcga aaggtatcca gaatataaat tatcttttct ttcagaaaac aagatgataa
     5401 aagtcgagtt ttcttctcag tttttactga gtaaagttta ttctatactt ttagagggaa
     5461 cgattggtta tattttactg gattcacttt acaaaggtac ttatcaatcc ttagaaaatc
     5521 ttagtcaaaa aattatataa gtttgcgtac gattcaaaga aaattaagag aacttaatac
     5581 tattttgaaa aactatcatt tatcttgcag cttgaaaaaa ggtaatccga tagaaggaga
     5641 agaataccgt atccgatact tttttcactt gatgtattgg cagtcattcg acgaaaatca
     5701 ttctgatata ttatcaagta taaaaaataa acgtcaacta ttagaaaaat ttgcgaaaca
     5761 tgcgccatat acgcgtagta tagatagaaa gaaatggatc agcctactaa gaatttcgtt
     5821 gcaacgaatg aaacaaggcc actcgattac gtatgtccca gaagagatga acaaatttgt
     5881 gcatccattg ataacaaaag aagagtttac gcattacata ctagagcctt tttttgaagc
     5941 aaacttctgg tcgatgaatc acatcactcc aacagagcaa gcttaccttt attttatgtt
     6001 tggtgtaatg aacacctatt tggaagaaga tttgttggat actccagata aagtacaaga
     6061 aagttggggc tggattgaat cgtctattgt agaaaatttt gaaaaaaact tcaagatttg
     6121 ttttacaact tcagaaaaaa attatctata tgtcaatttg gcaatgatcc atttgtattc
     6181 aagggttttt gggacaaaaa agaaggtgga tgcttttgga aaatcagcag aagacagaga
     6241 ttttcagcat atatttccag tgatgtatcc aatcatgcaa cagtttttta gtggggtagc
     6301 tcgcagaatt ccagagctta atacttatta tgaaaagaac agacggttga tttttcaata
     6361 ctgtatgtta gtcagggata tttttataaa acacgaacaa cctgtcatat tctatattca
     6421 atcaaagtat ggtaaaacac aggaattatg ggcaaagaaa aggatattat ctatgactga
     6481 acgcccaata aaatatagtg catccgctga tcagttacct gatattgtca ttagcgatta
     6541 tcctatcgac aaaaaggcat atgatcaaac gaatacacag atattttact ggaatggaca
     6601 acctacaaaa aatgattggg cgcgtctgaa agctgaaatc ataaaaatca ggaatgagaa
     6661 aaatagatcc atattggaaa aattggattt cgttaattaa gaaaagcaga ttgtttatgc
     6721 caaaattaag acattgcaaa aagattattt tctttaaaat cgacaagatg accagaaagc
     6781 aagagttcgt tgagctcttg ctttctttta gttttagaaa ggaacatcct gattttatct
     6841 tcgctaaatc cagtcaaaag ttttgcagat tggtcgtaga taatcggttt tttcaataaa
     6901 gaaggggatg tacgaatctc aatatatagt tcactcaaac tgagttcatc tattctgtga
     6961 acaagatttt tataactaat agaatgagta gagagcattt cggtcacgcc gttgtttgtc
     7021 aattttagta agtggataaa ttcatttttg gttagtggtt gacgaattaa gttctgttcg
     7081 ataaacggta tatcatggac gataagccaa cgttttgcgc gtcttgaaga cgagcagcca
     7141 gaacctgtat acaatttaat catcatacat tcctccaata gctaagtatt tatgaaataa
     7201 taacatgaag aaatagtcga aaagtgccat ttagaggtgt aaaataagaa aatgttgtca
     7261 cgtataatgg aactagctag ataagaaata ctattaaatc aagaaaaaag aaagaaggca
     7321 caagtatgtc aaacacagcc gacgcaatag tagtgatcga tttacaaaaa ggggtatgtt
     7381 ttgattcttc gccgatacac gaattagaaa cagtgacagc attagtgaat aaaagaatca
     7441 aaacatacga aacgattggg aaaccagtta ttttcgttca acacgaagat gctcaattag
     7501 tgccacaaac aaaaccatgg gagctattgt cggaattatc tattcctgag cacagttatt
     7561 ttgtgagaaa aacccatgcg aattcatttt ataagactaa tttgagtgtt ttattaactg
     7621 aattggactg tcattcgatc gaattatgtg gggcacaagc agagtattgt atagatacga
     7681 ctgtgaagtt tgctcatgga ttaggatacc agcttatgat gtgtagagga gcaacgacaa
     7741 cttatgataa tccttttatg aaagcaaagg aaacaatcgc tttttacgag aaaatttggg
     7801 ataaacgatt tctgacattt ttggagcaat aagttgtttc ttcgtttaaa aaaagcagaa
     7861 tggatactgg tttatccatt ctgctttatt acgtaaaatg agaaaataat cctgttctat
     7921 ttttatggga taaaccattt attttttgta taaaaaatgt ttgcgtttac atataattct
     7981 cctagaaaca taaaaaggag tgaatagctt gagaacaaaa caagtattaa agggagcgtt
     8041 ggtcatctta tttgctgtaa gtacaagcgt cccgcaatgg acagtatatg gagagagcat
     8101 ttcttccgaa tcggaatctc gggaggtaga aaccacagaa actactgatg ctggaaatga
     8161 aacgaaatcg agtacttcac aaacagaaac aaatactgaa acaacggttg agacatctgt
     8221 tactagcaca gatcaagaaa cgaccgctac cacagataac caacaaacag aagaagcaac
     8281 agctacaaaa atgtcaaacg acgaatcctc ttctgctaaa gtaagtgaca aagagaatgg
     8341 gacagaaaat gatgatatcg gtaaaggtga aaatttcaat ggtaaagaat atttgaatta
     8401 cttcggtaaa cagatgacag aaatacttaa tggaccaaca gaagacatca agtcaaaatt
     8461 aatagagtta ttagtcactg agcttgctgt taatgattat ggtgtagata aagagattat
     8521 caaacatctg attgctactt atctattgta cgatatcgct tttgcaaaaa acagtgtgtt
     8581 gatgtcgatg cttggaggta cagatgatgg gacaacgcct acagaaaaca caggcttcta
     8641 tccaaataca aaacaacttc atcaaactgt actaaacaaa caagatggca aaatgatgga
     8701 tgtatatgca tattatgtcg atcaaggttc agataaaaca gtcatgatcc atggcggatt
     8761 tcgagggaat tggaataacg ggatcgttac agaagaatac aatgattttt ataaggccgg
     8821 gtataattta ttatttgttg attccagagc gactggtaat agcggaggcg attatgtgac
     8881 ctatggacaa tatgagtcag atgacgtcct ttactggata aatcaagaag ttcgagagcg
     8941 gccatctcaa aagattttac tttatggtgg ttcgatggga gcagctacga tgatgtctgt
     9001 attagcgaaa gatattcctg tcaatgtcaa aggaattatt gaaaattgcg gatttgcatc
     9061 aattgatgaa caactacgtt ttacgtacag ccagacagta gcccccgcac taccggatgc
     9121 cataaaaaat cagttagaca tcattgggga tcaagagcat gaagatttat ttatgggcct
     9181 tttgaaacaa tactattttg atcaggagat gcatttggat acaaaagctg ctctacctac
     9241 gattggaatg tcagattcat tgcctaaatt gattatccat ggaacagcag atgatgtagt
     9301 acctgtgtct aacgcgcaaa aattatatga gttatcagga gggtataaag atcttttatt
     9361 agtagaagga gcagggcatg gaaaggcaca ggaagttgac catgctgcct acacgaaaca
     9421 tgtaacggac tttttgaaag tagtgttcca tgatcagata aacgtaaagt atgtggatga
     9481 aaacaataaa agtctactca aagaccagga cgaaatccag ctttacggag cttatggaga
     9541 aaattatatg acagagcaaa agacattcga aggatacgaa ttagcaaatg ttgaaggacc
     9601 gactgaaggg atttttaatg agacgatacc aactattatt ttctattaca aaaaaatccc
     9661 agtagctcca ccaaaaaagc aagatccgac agagaatgca gacaataagg gaaagaatga
     9721 acctcgagag gatcaatcaa gtgatgatac gcaaccgatt cagatgacag tgaaagtaca
     9781 aaaaaagaca gatgcaaaaa aaagaatgtt gccgaaaact ggagaaaaga aacattcgct
     9841 tctcatttat tcaggtggat ttttatctgt tagcacgatt atcacatgga tttggcgaag
     9901 aaagagaggg taaatgaatg aaatggttca agcgaatagt gattgcagta ccagttatca
     9961 tcgtagttat tttagtcact gtatttttga ttaatcaaat cacaccaaag ccgattagct
    10021 tgcttgttag aaaacaattt gacacaagtg aaaaagaaca agtatatgcc cgaccagatg
    10081 atttccagca aaaaatcgaa caaatcgata taaaaaaaga aatatcctat ccttcaaagt
    10141 acaaaaataa taatatggat atctacacac cgaaagtgga aaagaaaaaa ttaccgattc
    10201 ttttctggat gcatggaggc gcatatgtag gaggggataa aaatgattgt cgtgattatt
    10261 tggaatatct atgttcagac acgcagcaaa tcatcgtaaa tatagattat gaacgatcac
    10321 cggaagcaaa acatcctaca ccagttatcc aattgaacga agccattcaa gcagctaaaa
    10381 aagagataaa agatcaagca gattggtcga atatttcgat tggtggaaat tcggcaggtg
    10441 cacaaattag tggagaatat ttgttagcac ttcaaaatga agaaattaaa aaagctgatc
    10501 atcttgatcc gactttggag aacaagcaga ttacgaagtt tatttctttg tctggtctgt
    10561 tggatccttc tgcacttact caagtaagtg ataaaatctc tagctttctt tatgaaaaat
    10621 gtggatgggc ttactttgat aataaggact ttgaacaatc agagaaggtc aaaagtttag
    10681 ccttggtcag acatgcggat cgttggacgc aaaacacatt cttaacggat ggaaatacaa
    10741 atacgtttac gaaacaaatg gaagaagtaa cttctgtatt tgagaaagcg ggggtgaaag
    10801 tcacaaaagt agattatgcg gagaaaaatg ctgttttgaa ccatgaatat cagtttgatt
    10861 tcagcaataa acaagcgcaa gaaacatatc aaaagttagt aatgtttttc aaggagtaaa
    10921 gtcttatgat gcaaaagagg ttgtaatctg tcacttagct ccaagtcaca aggagcaatt
    10981 tcaataaaca agcttttcat ggtttatgaa tattgtaact tgtgactgag gagttagaga
    11041 tggcaagggg caccttttat tcgaaatttg ggaatatcac acgattattg tcacgatctc
    11101 ttttacgtaa attttataag tcagatttgt tgttttgatc gtttaaaaaa agcaacaaat
    11161 ccgaagcctg cggaggcaag aagggacaaa aagccgagtg gtaagcaaaa gaatgctggt
    11221 tccactaata ttccgttgtt attgatataa ggctgggaac aaagatgaat agcaaataaa
    11281 atcaatgcac tagcgaaaaa aagcaaagaa atatggatag ctttcataag cagcacctcc
    11341 ttttttataa ttgtatgata aatagattaa gaatctataa atgtgacaag tttgttagga
    11401 tgtgaataaa atgatcatct agcgaacttg aaacaaagat ggctaatgat aagcaattaa
    11461 tgattcagca atcagatagg tacaaagttt accatcatac gtctagacca ataatagaaa
    11521 atatgggaat ataatagaaa agatttagga tacccccaaa agaatttccc caaaaattcg
    11581 atgtatccta aactttttta ttttacataa caatcatatc aactgtgaaa taaaattaca
    11641 aatgataacc taaaatgagg ccaaaagcca gtgttatcaa ggaaatactt aattcaccaa
    11701 aatttttttt aattttttag aaaataagaa acaaaaataa taaaaaaaag aaaagataaa
    11761 ttttttacta ctgtatacta actttgttat cgcatctaac aagtaactcg aataatacca
    11821 atacaacctt ctccaaagat gcgatgacat acgttctaac ccaccgacta ttgatgttct
    11881 gtaccaccct tgcagaacca aaaaagccac taccaaatat ttggtagtgg ctttttttgt
    11941 tttatggatt tttcgctttt tctgttatgg tgctattctt tgatttcaga aatagtatgt
    12001 cgaactttag cgatagaacg acgtgattct ttttcgttgt aaaacatcaa aatcacatat
    12061 agagagtcca aaatgcttag ttgagaaata cgggaagcta aagcttcaga tcggaattct
    12121 gtttcttcag aaatagaaat aaaaacgaca tcgccaagtt tagcaaggga agaattagct
    12181 tggctagtaa taacgattgt ttttgctcca gtttttctta cggtttcagc aatatggatt
    12241 gtttcttttg attgccctgt gtgagaaatc aaaacagcac agtctttttc cgtcaataaa
    12301 gaagcttcca tcaattgaat atggtaatcc gtgctgtgag aagttgcgat aggggaacgc
    12361 aaaaatttat gataagcgtc agttgctaca atctctgaac cgccaacacc gaaaaaatag
    12421 acacgtttag attcattgat aatagtagcc gcttttttta gatccttttg ttgtaacaac
    12481 ttttttgtgt catttaaagt tgaaatacta gactcgaaca ccttttgcgc catagtgagc
    12541 tcagtatctg ttttctgaat attttcgtgg atagaaatag cagatagatc attttcttgg
    12601 atcaacatgg ccattttaaa atctttaaat ccattataac caagtttttt tgtaaattga
    12661 aaaaaagtag agtcagcaat gcccagttca cttgctaaat tgctaatagg actatgactt
    12721 actttgcttg gattttctaa aatataatcg gcgatagcct gttcttttgg gctgaagtcg
    12781 ttgtacattg ttttgatttg tagtcttatg gagacgctca tgagaattcc tcattttcat
    12841 aataagatta tcattttttc cgttctcatt aatgttaaag atacaaggag agtaggaaaa
    12901 aatcaaacaa aaaatatttt tgtaaaatct atttacaaaa aaattttttt gtataatgag
    12961 ggtataagaa atggaggcgc tatcatggaa attggtgtaa ttggtttggg aaaaatggga
    13021 ttgaatttag ctttaaactt aaaagagcac catcacaaag tacaaggact cgacatttca
    13081 agtacagctg ttagtcaggc aagagaaaat gacattcctg cttatcaaga gatctctgaa
    13141 tttctagcta cttttgagca gaaaaaaatc atctggttaa tgcttcctgc tggagagata
    13201 accgaaaaca tgttaagaaa actttttcca aaactcggac caggagacat tattattgaa
    13261 ggcggaaact cttattataa agattcaatc cgtcgagcag aagagtttca gaaaaatgaa
    13321 atcggttact tggattgtgg gacttctgga ggaatcgaag gagcacgaca tcaagcctgc
    13381 ttaatgattg gaggagataa agaaactttt ctatccgtgg aacaattatt caaagatgta
    13441 gcaattgaaa gaggatatct gtatgctggt gcatccggaa gcggacactt cttgaaaatg
    13501 atccataatg gaatcgaata tggaatgatg caagcaatcg gtgaaggatt ccaacttgta
    13561 caaaaaagtg agtatgcttt tgacttggaa caagtcgcac gtgtttggaa tcatggttcg
    13621 gtgattcgcg gctggctgat ggaaatcatg gaagatcagt taaacgaaca tcccaattta
    13681 agtaattatc ggggaatcgt ttctgcatca ggtgaagcaa aatggacggt tgaaacagcg
    13741 ctagaaatgg ctgttccagt tccagtaatt gcaactagtt tgttcatgag gaatctttca
    13801 caggaagaag atagtttttc agcaaaagta gtttcagcac taagaaatgg gtttggcgga
    13861 catgaaatag ttgaaaaggg tgagtaaaat gccagattat catatagcaa tcgttaattc
    13921 cagcagtttc ggaagagtct ttccacacca cttggaaaga ttaaagtcag ttggagacgt
    13981 tcatttcttt actgttgatc aagagattcc aggaaaagaa ctagcggagt tacttaaagg
    14041 attcaatatc attattgcaa gtgttacgcc attttttcga cgagattttt ttgaaaacaa
    14101 agatgaactg ctattgattt cccgacatgg tattggttac aacaatattg atttaaaagc
    14161 agcaaaagaa caccagacaa tcgtatctat catacctgct ttagttgaac gaaatgctgt
    14221 agcagaaaat aatataacta atttgttgtc catgcttcga cgcacgacac aagcacaagc
    14281 aagagtcaag gacgatcgat gggaagatcg ggcggagttc gtcggtagga cgctgtttaa
    14341 caaaacagtt ggtgtgattg gtgcaggaaa taccggaagc ggagtaatcg aaattttgcg
    14401 taacggtttt cgctgtgatg ttctagctta tgatccttac aaatcagatt tatacatcga
    14461 aagctttggt gcaaaaaaag tagaacttca agaattactg gaacaatcag atatcatttg
    14521 tttatgtgcg aatttgacag aagagaacta tcacatgatt tctgataaag aagtcgatca
    14581 gatgaaagaa aatgtttatc tatctaatag tgcaagaggc gctctagttg atgaagaagc
    14641 gatgattaat ggtctgaaaa gcggaaagat cgcaggatat gccacagatg ttttagaaga
    14701 agaaccggga cgtcaagatc atccgtattt agcgtttgaa caagtagtga ttacgccaca
    14761 tatatcggcc tacacaaggg aatgtttaga agcaatgggt gaaaaatgtg tcagagatgt
    14821 cgaacagatc agtcagcacc ttcttcctga acgttctgtc cagacaaaga gcaactatat
    14881 aaaaggatga tgaagaaaat ggtgaacggt ttaaaagtga aaacaggccc tcaattttat
    14941 ttatatgaag aaggtggtat ttcaaaagtt tctgatctct tgaaatcata cggcgcgaaa
    15001 agagtactgg tcacccacgg aacggtatca tgggaaaagg cgctacctaa attagtattt
    15061 ttgaatgacg aaacgattca atttttttat catcgttatt ctggagaatg tagttatgct
    15121 gaagccagac gaatagctac aatcatcaaa aaaatgaaat cgatttcgta gttggcgttg
    15181 gcggaggaaa actgaccgat ctagtgggtt atagtgccaa tcttgcaaga atcgatttcg
    15241 gagtcatccc tactttagct agtaattgtg ctccatggac acctcttgct gtcatgtata
    15301 aggagaatgg acaagcagaa gggaaaacag aacactttta cagacaagct gcttttttta
    15361 taacagatcc ccaattagtg attgattccc ctgttccata tttcattgcc gggttagcag
    15421 atacactagc aaaatggtat gaatctgagc tcatcttaga acaagatttt ttgcaagatg
    15481 aatcattttt gaagctagca caagatactg cgaagatctg caaagaagag attttgaatc
    15541 attctgctaa agcaatccaa gatatggcac aaagaaaact gacatcggag ttcaaacagc
    15601 tttcagagat tatctttgct gttgctggtt tagttggagg attaggagat aaatacgcaa
    15661 gaaatgtggc agcacatgct atgcatgacg ctatcagtaa atatattccg gaaagccatc
    15721 gttttctaca tggagaaaaa gtagcgtatg ggattttcta tcaactggca ctagaaggtc
    15781 gctgggaaaa aatagacgag ttatatcctt tgtatcggga attagaactg ccggtttctc
    15841 tagaacaaat gggattattt ccgaaagata aacagataat agataaaatg gctgaattta
    15901 ttgattcgaa agagaaagtg catttgattc ctatagaggt aacacagaaa tcactgatac
    15961 agtcaatgat ggaactggaa ggttatatca gaaagatctg aaggaggagg acacatggaa
    16021 acattaagtg tagtacaaag tttgttgatt gctttatggg tagccgcggt tatgtcccgc
    16081 tggttaggtg gaggagccac gttgacctta cgtttttctc ctttgatgac tggattagtc
    16141 gtcgggttga tcatggggga agttgcacaa gcgatgattg ttacagccgc tttgcaaatg
    16201 atttatatgg gggttttttc tccaggagga tcaatgccag ctgaaccatc tattgcagca
    16261 gctatcgcag taccagtcgc attattaggg gatctgtcgc cagaagcagc gattgccgtt
    16321 gcggtgccag tcggattgct cggaagttac ttgtatcagt ttcgtttttt catcaatact
    16381 tttttaggta aatatacaga caaagctgta gaagatttga atacaaaagc tatggcccga
    16441 tcaattattc tttatccaac ggttgcctca tttttattat tcgttccgct tgttttcatt
    16501 gctttatatt taggggcgcc attgatcgct gatgtaatca aagctttaga gggaacagtc
    16561 gttatccaca ttcttgaagt agtaggtgga gggttagcgg caatcggtat cgcgacaact
    16621 gtatacgtta tcggtcgaaa agattatctg gtctttttct tccttgctta ttttatgagt
    16681 gttgttttga aatctttaga tattacgatg gttacttatg ccatttttgg gattattatc
    16741 gctttgatct ttgtccagtc gcaaaaaggg aaacaagtac agcctgccac aagcaataac
    16801 aattcagcat cttttgatga cgaagatgac tatgatgacg gattttaatt taggaggaat
    16861 gggaaatggc tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag
    16921 atgtaacaaa agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac
    16981 gttacttggc gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag
    17041 atgaagactc tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa
    17101 gctggggtgg cggtgtgatt actggcctaa tgtcttctat ggaacagcaa agagcagaag
    17161 aagagtatga agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg
    17221 gattgatggg ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata
    17281 tttttatcgc aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc
    17341 cttttatctg ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt
    17401 tcactatggg ccgtaatgct acaggcctaa agcaaaacac tggtgttcag acagcgattg
    17461 aaatgctatc agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag
    17521 tagaatcttc attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg
    17581 atcaaatcgt gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata
    17641 cgaaaaaagg attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc
    17701 tagcaggaat cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt
    17761 tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa
    17821 gtggagtgaa ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag
    17881 atctttacaa aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga
    17941 aagctaaatt ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa
    18001 aaacagtcca aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata
    18061 ttggagggat tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt
    18121 taaataaatc agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata
    18181 tttattttca agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt
    18241 ttgatttgtc ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga
    18301 acgagctgtt tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa
    18361 aaaagagtaa acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag
    18421 atgtgacagt cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag
    18481 atatcgaaaa agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag
    18541 gaagttattt catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat
    18601 tgtccttcat tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc
    18661 ctcatcacgt attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga
    18721 gctacttttt cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc
    18781 gaaatacttc atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg
    18841 tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat
    18901 cgggtatgaa aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc
    18961 tacagttttc gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt
    19021 tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct
    19081 atttgaccaa aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta
    19141 ttgctctttt tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa
    19201 tcatgatcgt tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg
    19261 tcgggacatc gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat
    19321 tcggagagaa ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat
    19381 ttgttgatct aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga
    19441 ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag
    19501 aagcaatcaa tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca
    19561 aacaaggaaa agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg
    19621 aggacgattt ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa
    19681 aaaggggatt cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt
    19741 ggaactttgc tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg
    19801 aaagcatctg ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac
    19861 gatgcaatca aggcagggac agatgaacga gattactata ttgataacta tatacgaaca
    19921 atcaaaaact tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt
    19981 ggatgggcga aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac
    20041 gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag
    20101 tcaaagggat tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg
    20161 gaaaaactgt atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta
    20221 gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat
    20281 ccgccatggg aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa
    20341 attctaaatg cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg
    20401 gcaaatccgg aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt
    20461 gtccacttca gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc
    20521 tctacagaag gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc
    20581 gacggtccta tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga
    20641 tatggcctgt atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata
    20701 agtaaggaaa aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat
    20761 ggatggcctt ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca
    20821 aaagtatgat tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat
    20881 ggcgataaga gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg
    20941 agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa
    21001 aaaaggagca gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc
    21061 ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt
    21121 gtttgatcaa atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt
    21181 tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc
    21241 gattgcaggg atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca
    21301 tttgataggg ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata
    21361 tgaatccaat aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga
    21421 aagtaaaggg ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc
    21481 ctgcacgact gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt
    21541 atttgtagtg aacaatttag aacaaacaca aaacgcatct actgttatct accagtagat
    21601 gcgtttaaag agaagaacga gaatcgtcca tatgagggtt tcc
//
LOCUS       DAFBQH010000040        21136 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.040, whole genome shotgun sequence.
ACCESSION   DAFBQH010000040 DAFBQH010000000
VERSION     DAFBQH010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.040"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(87..275)
                     /locus_tag="LK694_001647"
     CDS             complement(87..275)
                     /locus_tag="LK694_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBK5052514.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            545..907
                     /locus_tag="LK694_001648"
     CDS             545..907
                     /locus_tag="LK694_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBK5052515.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            959..2641
                     /locus_tag="LK694_001649"
     CDS             959..2641
                     /locus_tag="LK694_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBK5052516.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2758..4794
                     /gene="recG"
                     /locus_tag="LK694_001650"
     CDS             2758..4794
                     /gene="recG"
                     /locus_tag="LK694_001650"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBK5052517.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4843..5844
                     /gene="plsX"
                     /locus_tag="LK694_001651"
     CDS             4843..5844
                     /gene="plsX"
                     /locus_tag="LK694_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBK5052518.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5966..6208
                     /gene="acpP"
                     /locus_tag="LK694_001652"
     CDS             5966..6208
                     /gene="acpP"
                     /locus_tag="LK694_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBK5052519.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6476..7480
                     /locus_tag="LK694_001653"
     CDS             6476..7480
                     /locus_tag="LK694_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5052520.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7481..8425
                     /locus_tag="LK694_001654"
     CDS             7481..8425
                     /locus_tag="LK694_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5052521.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8425..9387
                     /locus_tag="LK694_001655"
     CDS             8425..9387
                     /locus_tag="LK694_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5052522.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9414..10328
                     /locus_tag="LK694_001656"
     CDS             9414..10328
                     /locus_tag="LK694_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5052523.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10354..12135
                     /locus_tag="LK694_001657"
     CDS             10354..12135
                     /locus_tag="LK694_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK5052524.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12483..13169
                     /locus_tag="LK694_001658"
     CDS             12483..13169
                     /locus_tag="LK694_001658"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBK5052525.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13189..16770
                     /gene="smc"
                     /locus_tag="LK694_001659"
     CDS             13189..16770
                     /gene="smc"
                     /locus_tag="LK694_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBK5052526.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEVAEKETEIQKAEASLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYVQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16779..17588
                     /locus_tag="LK694_001660"
     CDS             16779..17588
                     /locus_tag="LK694_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK5052527.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMVHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17600..18598
                     /gene="ftsY"
                     /locus_tag="LK694_001661"
     CDS             17600..18598
                     /gene="ftsY"
                     /locus_tag="LK694_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBK5052528.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKIEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18785..21052
                     /gene="gshAB"
                     /locus_tag="LK694_001662"
     CDS             18785..21052
                     /gene="gshAB"
                     /locus_tag="LK694_001662"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBK5052529.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEAIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
BASE COUNT         7168 a         3756 c         4683 g         5529 t
ORIGIN      
        1 aagggtagga atcaaaagtc ccacccttaa aaatttttat taaataaaaa accgttcact
       61 atctgaacgg tccaagtatc attcaattaa acgcgttcaa cttttcctga tttcaaagca
      121 cgagttgaca cccaaacttt tttaggtttt ccgtcgacca taacgcgaac tttttgtaag
      181 ttaggtttaa cagtacgttt tgttgagttc atagcgtgtg aacggttgtt tccacttttt
      241 gtcttgcgac ctgtaaagta acaaactttt gccatggtaa tttcctcctt tgccttgatt
      301 tggagcaata gaataattca gctcatacca gactaacata tcatatagaa tggaaatttg
      361 caagtacatt ttcatagata caattgacaa ttgagaagaa taattgtaaa ttaaacaagg
      421 ttatacctga aattttgcaa ggtttttgag tactttgggt aactgccttt tatttcaagt
      481 tgcgctatga taaaatatga tagaagaaaa atgggagttt tttacccgtt aaggaggctc
      541 attcatggct gtaaaaattc aaacgtcagc tggaaccatt gaaattacca atgatgtaat
      601 tgcaacagtt gttggtggcg ccgcgacaga tgtgttcggt gttgtaggaa tggccagcaa
      661 aaatcaaatt aaagataata ttactgagat tttgcgtaaa gaaaattact caagaggagt
      721 agttgtccgc caagaagata atggtattgc tgtagatgtt tatacgatta tcagctatgg
      781 tacgaaaatc tctgaagttt cccgcaacgt ccaagaaaaa gtgaaataca atctagaggc
      841 attattaggc gtaactgcca attcagttaa tgtctttgta caaggtgttc gtgtactgcc
      901 agactaggca ggagactggc tggtaactca gcagtcgatc tgaaggagga ttatctaggt
      961 gagtgtaaca gaaataagtg caagccagtt ccaggaaatg gtccaggcag gtgcgaatcg
     1021 tctgcaagta aatgcagaat acgtcaattc attaaatgta ttccctgtgc cagatggcga
     1081 tacgggaaca aatatgaatc tatctatgac tagtggagct aaggcagtag tagattcaac
     1141 ctcagaaaaa gtaggagaat tagcagcaat gctttcaaaa ggattgctga tgggtgcgcg
     1201 cggaaattcc ggcgttattt tgtctcaatt gtttcgtgga ttttctaagc aaattccaga
     1261 tgttgtctca ttaaatgctt ccgatcttgc tgcagccttc actcatggag ttgaaactgc
     1321 gtataaagca gtaatgaaac cagtagaagg aacgattttg acagtagcta gagaagcagc
     1381 gaaagctgga gaaaagaaag caaaaagtac tgatgatgtc gttgaagtaa tgacagctgt
     1441 tgtaaaaggg gcaaagcgtg ctttagctaa aactccagac ttgcttccag tcctaaaaga
     1501 agtcggtgtc gtagacagtg gagggcaagg actgctgttt gtttatgagg gctttttaag
     1561 tgcattgaat ggagaattcc aagcggatga aacatatgaa ccttctccag cagaaatgga
     1621 tgatatggtc aatgcagaac atcatcgcag tgtccaaggt caaatggcga cagaagatat
     1681 ccattttggt tactgtacag aaatcatggt aaaaatcgga gaaggaccta cagtcgatag
     1741 tacattcgat tacgaagaat tccgtaacta tttagatggt ataggagatt ctttacttgt
     1801 ggtcaatgat gacgagatca tcaaagtcca cgtgcataca gaaaacccag gagaagtctt
     1861 gaattatgga caaaaattcg gttcattagt caaaatcaaa gtcgacaaca tgcgcttgca
     1921 gcatgagacg attttggaac acgatgaaga agtgaacgag tttgcgaaaa aagaaacacc
     1981 acgtatcccg tatggtatcg tagcgatcgc tgctggtaaa ggcgtccaag agctgtttga
     2041 aagtttaggc gctaattatg ttatcagtgg tgggcaaaca atgaatccaa gtacggaaga
     2101 cattgttaaa gcgattcaag aagtaaacgc agataaagtg attgtcttgc caaacaataa
     2161 gaacatcttc atggcagctg atcaagcagc agaagtagca gatatcccag tagcagttgt
     2221 tccttcaaga acgatttccc aaggattgac agctatgctt tcattcaatg atcaagcttc
     2281 tttagaagaa aacaaagaaa cgatgacaga aatgttgtct agcgttgtga gcggtcaagt
     2341 cactcacgcg atcagagata cctcaattga cggtgtagag atcaaagaag gagacttttt
     2401 gggcatgatc gatggaaaaa tcctgatctc taacccagat atcacagcaa cagcgttaag
     2461 tacactagaa caaatgatca atgaggacac agagatcgta acgatcttgg caggtgatga
     2521 aggaacagtt gaacaagcaa atgaatttgc tgagaaacta acagaaaaac atcctgattt
     2581 ggaaatcgag atccaccaag gagatcagcc tgtctatcct tatctatttt ctgctgaata
     2641 atttctgtgt cagaatatct ttctaacaca gtattcattc ataaaaggcc ttcggttagt
     2701 gatcaggtat gatcgctgac caagtccttt ttttgttaag ggagggagga aaaatttatg
     2761 cgttctttag atgatccaat cactatgctg gcaggagtcg gagaaaaaag agcagctagt
     2821 cttgccagct tgggcatcca aacgattgaa gacttactga cttattatcc tttccgctac
     2881 gaagatattc aggaaagaaa tctaaacgag atccaagatc aagaaaaggt tactttgaaa
     2941 ggaatcgtag tctcgcctcc tgtgatgaat cgatttggtt ataaaaaaag ccgattgcaa
     3001 tttcgtatga tgcaagaaca tgatgtgttc aacgtttctt tttttaatca accttatcta
     3061 aaagaaaaag tcattttatc ggaggaaatc gccgtatacg gtaaatggga tgcaaagcgt
     3121 aaagcattga acgggatgaa gattttaggt tctcaaacaa tggatgactt ttcgccgata
     3181 tatcacgtca acaaatcaat tcgtcaaacg acattagttg atctaatcaa aaaagggttt
     3241 caagaatacg gcacattgat tgaagaaaat ctgccaaaca accttgtgga gaaatatcga
     3301 ctgcttgacc gagcttctgc tgtaagaagt atgcattttc caaaagacca cgaagaaaac
     3361 catcaagcca aacgacgtgt cgtttttgaa gagttctttt tatttcaaat gcgtcttcaa
     3421 ggattgaaaa aagcagaaaa agcagaaacg aatggtttgg agatccttta tgatgtccaa
     3481 aaattgaaag aatttactca aaagctacct tttgaactga caaatgcaca aaaacgtgta
     3541 acgaatgaaa tctgtcggga tcttcgcagt ccgcagcata tgcagcgact tttgcaggga
     3601 gatgtaggta gcggaaaaac agtggttgcc gcgattgcgt tatacgctac cgttacagca
     3661 ggtttccaag gtgcgttgat ggtgcctaca gaaatcttgg cacaacaaca tatggaaagc
     3721 ttgcaacagc tgtttgatcc caatgaagta accatcgcat tgcttacggg gtcaacgaaa
     3781 gcaaaagaac gtcgtgagct ccttgaaaaa ctagagcaag gggaaattaa catcgtgatc
     3841 ggcacacatg cattgatcca agatggagtc gaatttcaga atttaggtct agttattacg
     3901 gatgagcagc accgttttgg cgtgaaccag cggaaagtac ttcgtgaaaa aggctggcga
     3961 ccagatgtct tatttatgac agcgactccg attcctagaa cattagctat tacagcatat
     4021 ggtgagatgg atgtatctat cattgatgaa cttccagctg gacgtatccc gatcgaaaca
     4081 cgctgggtaa gaacacctca attggacagt gtactggatt ggacgaaaaa agaactggca
     4141 agaggacatc aaatgtatgt catctgtccg ctgattgaag aatcagaagc attagatgta
     4201 aaaaatgcag tggaaatcta tgaaaaactt cgtgaactat ttgcaccaaa atatgaagtt
     4261 ggcttgttac atggtaaaat gaagaatcag gaaaaagaag taatcatgga agccttcaaa
     4321 gacaaccaga tgcagatatt agtttcgact actgtaattg aagtaggggt aaacgtgccg
     4381 aacgcgaccg tcatgctgat catggatgcg gatcgatttg gattggcaca gctgcatcaa
     4441 cttcgtggac gtgttggacg cggaagtgat gcttcctact gtatcttagt tgctaatcct
     4501 aaaaatgaac taggtgtgga acggatgaaa atcatgacag agaccaccaa tggttttgta
     4561 ctaagcgaaa aagatctgga attacgtggt cctggtgaag tatttggctt ccgtcaatct
     4621 ggtctgcctc aatttgcgat tgctgacttg gtgacggatg gaaatgtttt ggaggtagcc
     4681 agagaagagg ctgcagccct ttggcagata aaggattggc agatgcttcc tgagtatcag
     4741 cctttagcgc aaagcctcca aatagaaaat caggataatc gttatttcga ttagcagact
     4801 gtgctatact ttatactgat gacggaaaaa ggagtggaaa caatgaaaat tgccgtagat
     4861 gcaatgggcg gagatcacgc acctcaagca attgtagaag gcgtggcttt agcgcagcaa
     4921 gattttccag aagtagaatt cttactttac ggaaaagaag cagaaatcaa gaaatactta
     4981 acaaatgaaa aaaacatcac gattatccat acagacgaaa aaatcaatag tgatgatgag
     5041 ccagtaaaag caattcgacg aaaaaagact gcatcaatgg tccttgctgc acaagcagtg
     5101 aaaaacggtg aagcagatgc tgtattttca gcagggaata caggcgcgct actagcagca
     5161 gggttgttca tcgtaggacg tatcaaaaat atcgaacgcc ctggcttgat gtctaccttg
     5221 ccagtagtag ggaaagaagg tgccggtttc gatatgctgg atcttggcgc taacgcagaa
     5281 aacaaaccag aacatttgtt gcaatacggt attttaggtt ctttttatgc acgtaaagtc
     5341 cgaggtatcg gacgtcctcg tgtcgcactc ttaaacaacg gaacagaagc aacaaaaggt
     5401 agtgaagtga cgaaaaaagc ctatgaactc cttcagaatg aaacaagctt gaattttatt
     5461 ggaaacgtag aagctcgtga gctactcaat ggtgtggctg atgtagtggt cacagatgga
     5521 tttacaggca acgctgtttt gaaatctatt gaaggaacag caatgaatat gatgaatcta
     5581 ttaaaatctg ctattctaaa tgaagggatc aaaggaaaaa tgggcgctat gcttttaaaa
     5641 gacggcttac gttctttgaa agcagaaatg gattattcca aacatggcgg tgccgttttg
     5701 tttggcttga aagcaccagt cattaaaact catggagcaa caggccccga tgctgttcgc
     5761 tatacgatcc gccaaatcca cacaatgctt gagacagatg tagttggaca attagtcgaa
     5821 caatttgaaa aaagcgaaga atagtatttg gatatctgaa gcaatccgtt ccattgaaaa
     5881 aaaggtggac attcctgaaa aatgccagta aaattagcca tggtgtaggt aattgacaag
     5941 tgtatgattc ttggaggtga aaaagttgac tcgcgaagaa gtatttaaca aagtagcgaa
     6001 aattatttca aatcattttg agatagatac cgataaagtg acggatgaat tgaacatcaa
     6061 agatgattta aaagctgatt caattagtat tatggaattc gttttagagc ttgaagatga
     6121 attcggtacg gaaatttcag atgaagacgc tgaacaaatt gaaacagtcg gtggcgcagt
     6181 agattatatc agcagccatc ttaaataatt tcaaataaaa gcaaagaagg ataaaaagct
     6241 gcatacagct ttttatcctt ctttttttaa tctttttgac acgtaaaagt catttttctg
     6301 ataattctgg cgataaacag aaattatgtt gcaaagtaaa gcgattttct atataattat
     6361 taataattca ttacacaaag ataagaataa cctttgttat aaaacgcttt attctgtgta
     6421 atgtcctttt ttattaagaa aaacttccat ttaggaagca gagaggggta ttcgattgtc
     6481 cgataaccaa ttattagaag ttcagaacct acatacagga ttccgactca aagatgacta
     6541 ttacgatgca gtagatgacg tttcatttac tttagacaaa aacgagattt tagcaatcgt
     6601 tggggaatct gggtgcggga aaagtacctt ggctactacg attatgggat tacttgatcc
     6661 taataacaca aaaattacag gtgagatttt atataacgat ctgaatttaa tcgacctgaa
     6721 cgaaacgctc tataataaaa tccgcggaaa tgatatcgga atgattttcc aagatccgtt
     6781 gtctgcatta aatcctctca tgagaatcga agaccagatc aaagaaggac tttcctatca
     6841 cacaaagatg aatgcagaac agcgtcaagc tcgtgcacta gaattgctag aacaagtagg
     6901 gattcctaat cctgcgagag ttggacgcca atatccccat gaactatcag gcggtatgag
     6961 acaacgtgtc atcatcgcaa tcgcaatcgc ctgcaaacct ccaatcgtca ttgcagatga
     7021 accgacgaca gccctagatg taaccatcca agcacagatt cttgatttac tgaaagacct
     7081 tcaggaagaa acaaaaacag ggatcatctt gatcactcat gacttaggag tcgtagcaga
     7141 aatggctgat cgagtggcag tgatgtatgg cggacagttc gttgaagtag caggtgtaaa
     7201 agaactgttt gatcatccta agcatcctta tacacgctct ttactgaact ctataccaca
     7261 agaaggaaat catgatgcag aactccatgt gatcgaaggt gtcgttcctt ctctgaaaaa
     7321 catgcctcgt aaaggttgtc ggtttgcacc acgtattcca tggataccag caagcgatca
     7381 tgaagaagag cctatccttc atgaagtcgc accgaaccac ttggtacgtt gctcctgcta
     7441 taagcatttc cactttagag acgaaaaagg agaggtgtag atgacagaat taatcacaat
     7501 caaagacctg aaagttcatt accctattcg cagtggattc ttcaatcgag taacggatca
     7561 tgtatatgct gtagatggtg tggattttat tatcgaaaaa ggaaagacat atggtttagt
     7621 gggagaatct ggctctggaa aatctactac gggaaaagcc gttgtgggat tagaaaaggt
     7681 gacagccgga caaatcatgt atgaaggaaa agatgtaacg aagcgaagca accgcaaaaa
     7741 aatgggttat aacaaggatg tgcagatgat cttccaagat tcaatgtcca gtttgaatcc
     7801 taaaaagcga gtgttggata tcattgctga acccatccga aattttgaac ggttaagcga
     7861 tcaagaagaa aagaaaaagg taaaaggact gctagatatc gttgggatgc cggaagatgc
     7921 gctatataaa tatcctcatg aattttcagg cggacaacgt caacgtctag gagtagcccg
     7981 tgctgttgct accaatccaa aactgatcgt agccgatgaa cctgtatctg ctctggatct
     8041 atctgttcaa gcgcaagtat tgaatttcat gaaacgaatc cagcaagaat acggcctcag
     8101 ctatttattc atttcacatg atttaggtgt agtaaaacac atgtgcgaca atatcgcaat
     8161 tatgtacaaa ggtcgatttg tagaaatcgg tacaagagaa gacatttata atgacccgcg
     8221 acatatctat acgaaacgtc tgctttcagc tattccgcaa atcgatgtag aaaataggga
     8281 aagccacaaa gaaaatcgac gaagagtaga acaagagtat atccaaaacc aaaaagaata
     8341 ctatgatgcg actggtcgag tctacgattt gcgtacgatc acaactacac acaaagtggc
     8401 gttgaaagat ggaggtgcaa gctaatgtgg aaaacgattc tgcgacgtgt tttattgatg
     8461 atcccgcaag ttattatttt aagtgttctg atcttcttat tagcccaagc gatgccgggg
     8521 gatccattta ccggtttgat caatccaaat caggatccgg cagtgatcga acagatgcga
     8581 caagcagctg gcttgaatga cccatggtat gtccagtact tccgttggat cggaaatgct
     8641 ttgcaaggcg attttggtca aagtttctta tacaaacgct ctgttgctga cttgatcggc
     8701 gaacggatca tcaacacttt atatctatcg atcttgacag ttgtccttac ttatttgatt
     8761 gctgtgcctc tggggatgct tgctggccgg tatcaaaatt caatcttgga taagattgtc
     8821 gtaatctaca acttctttag ttttgctgtt cctttattta tcttcggctt gatcatgcta
     8881 tttgtttttg gttatcgttt aggctggttc ccaacaagtg gatcacaaac actaggtttg
     8941 caaggtggat ttatcactca atggatagac cgtttgaaat atattctgtt accatcaatt
     9001 acccaagcat ttttggcgac tgccgtcaca atccaatatc tgcgaagtga agtaatcgat
     9061 tcgaaatcgc tcgattttgt cagaacagcc cgttccaaag gggtaccgac gaataaagta
     9121 ttcagtcgac atattttccg aaatgcagcg ttgccgatgg cttctcaaat ggggtatgaa
     9181 atcacttcgt tgattgctgg ttctgtagtt atcgaaaaga tttttggtta tccgggagtc
     9241 ggaaaactat ttattgattc catcggccaa cgggactata ccgtgatcac atccttagta
     9301 ttgattttag gaatcgcgac gctagtgggg acattacttt cagatatcat catgagtatc
     9361 gttgacccgc gtattcggat tcaataaaac caatcagaaa ggagaatgga tcgatgagtg
     9421 aaaaaaacaa tacagcagtt caagaaagta ttcctcctat ggggatacga atgattgctc
     9481 gagaatttaa aaaagataaa attgctattt tttcattagg tttgctagtg gttttattat
     9541 tagtgatctt tatcggtgcg atgctgaccg atcaagacaa agtgatgtat gttagtatct
     9601 tcgacaaata cgctgcacca ggaacagttt cgacacaagg acaaaagttc attctgggag
     9661 cagatgaagg cggacgtgat gtattaggac aattgatcat aggtgctaga aactctgtta
     9721 cgatcggatt tgcgatcacg atccttactt cgatcatcgg ggttggatta gggatcatct
     9781 ctggctacta cggagggatc attgacaaca tcttgatgcg gatcgttgat tttatcatga
     9841 ttctaccgat catgctgatc attatcgtct tcgtatcgat tatttccaga tacaatgtct
     9901 ggtcctttat attcatcatg agtgcatttt attgggtagc taaagcccga ttattccgaa
     9961 gcaagacatt atctgaagca agaagagatt atgtcagtgc ctcaaaaaca atgggaacca
    10021 atgattttaa aatcatgttc cgcgaaatca tgccgaatct aagttcatta atcattacta
    10081 atttgacgat gaactttgcg gcaaacatcg gtatcgagac aactttgact ttcttaggtt
    10141 tcggcttgcc ggcaaatgta cctagtttag gtacattgat cggttatgca agtaacggag
    10201 aagtcttagt gaatcggcaa tggatctggt tacccgcatc aatccttatt ttagttttga
    10261 tgttgagtat caattatgtt ggacaagcat tcaagcgttc tgcagatgcg cgccaacggt
    10321 taggctagta tagaagcgaa gggggaaata ggaatgaaga aaacgatttt tggatttgca
    10381 gcgctgatgt ctgctgtcgt tcttgcagca tgcgggggcg gaggtacaga tacctctgga
    10441 aacaactctg gaggcaacgg caataacaaa gaggaattaa cttttccaat tgcaacaaaa
    10501 aataatgatg atgcaatcga agacggcgaa ttagaagctg cggtcgcaac ggacagtcag
    10561 tttaaaggat tgttccagtg ggagttttac caagattcat atgatgcagc ttttatggct
    10621 ccttctcatg aaccgctatt tgcaaacgat gacgatttca gaatcacaga cgacggagct
    10681 gcaacgcttg atctagatga agatacgaaa aaagcgacaa tcaaagtaaa agacaacgtc
    10741 aaatggtcag atggtgaaga tgtaacggcg gacgatgtga ttttccctta cgaaatcatt
    10801 gggaataaag attacactgg tatccgttat gacgacactt tccgtaacat cgtaggtatg
    10861 gaagaatatc atgatggaag tgccgacaca atctccggaa tcaaaaaaat agacgacaaa
    10921 acagttgaaa tcgaatataa agaagtaaac ccaagcatgc ttcaatcggg tggtggtatt
    10981 tggtcatatg cagcaccaaa acatacgtta gaaggtgtag caatcaaaga tatggaatca
    11041 agcgatcaag tacgtaaaaa tccagtgact tttggccctt attacatgag taatatcgtt
    11101 gcaggagaat cagtagaata cttgccaaac gagcactact ggaatggtac gcctaaattg
    11161 aaaaagatca cgatgaaatc tgtaccgatg gcaagtgcaa ctgaagcatt gaactctaaa
    11221 ttatatgata tgattttcca aatgccaaca gatacttacg atacctataa agaaattgct
    11281 ggctatacaa acttaggccg tgaacaaact tcttatactt atctaggatt caagttaggt
    11341 aaatggaatg cagatacaaa gagtgtggaa tacaatccag attcaaaaat ggctgataaa
    11401 tcattacgtc aagcaatggg ctatgcatta gataataatg cagtaggtga gcgtttctat
    11461 gcaggtttga gaagcaatgc gacttcattg attcctccag tattccagtc ataccatgat
    11521 gatgagatga aaggattcac acaagatcta gacaaagcga aaaaactatt agatgatgct
    11581 ggctacaaag atgtggatga cgatggttta agagaagaca aagatggaaa taaactaaca
    11641 atcaactttg cttcgatgtc cggtggggaa acagcgcaac cattggcaga ttactatatc
    11701 caacagtgga aagaaatcgg tttggatgtc caatacacaa caggtcgtct gatcgaattc
    11761 aattctttct atgataaatt agaaaatgat gatcctgaaa ttgatgttta ccaagctgct
    11821 tggaatacag gaactgatcc aaatccaagt ggactatggg gaccaaatgc tgcgttcaac
    11881 tacactcgtt atgaatcaga agaaaataca aaattgatca acgatatcag ttcaagcaaa
    11941 gcatttgatg aagagtacca aaaagaagca tatcaaaaat ggcaacaata tgcatttgaa
    12001 gaagcatttg cgattccaac gttgttccgt aatgaagtat tgccagtcaa cgaccgtgtc
    12061 aaagactggg attggtctta tgatgtacca aacccatggg caaatgtttc tgttacagca
    12121 gattcacgtt cataatcact aaaaatgacc gaaaaagggc gatgtccttt ttcggttttt
    12181 tgatattttt ctgtttattt ctctaatatt caggtcaagg cgctgatttc atgacttctc
    12241 caataaacgg tgttcaaagt ccagtttctc ggtattaagt caaatttatg caaaaatatg
    12301 gaaagccatt ttcacaaaat tttttcttca tactcgaaac taaacgaatt tttcacaact
    12361 tctcgtcaat cactaggatt attaaagctt tacttacctt ttgaccgaac catttcaaat
    12421 ttgacaaaaa accagtataa taaggaagta tcgaaaaaat atccataaaa ggaatgtgga
    12481 aaatggacaa ccagctgaca aaagaactaa aagaaaacta taacatcgtt tttcacgatc
    12541 taaatttgct tgaacaagct tttacccatt catcctatgt gaatgagcat cgcaatctgc
    12601 aattatcgga taatgagcga cttgaatttt taggagatgc cgttttagaa ctgatggttt
    12661 ctcagtattt attccgttta tatcctcatt tgccagaagg aaaattgaca aaaacacgag
    12721 ctgcgatcgt tcgcgaagac agcttgtcga aatttgccaa agaatgtcac tttgatcaat
    12781 atgttttgtt gggcaaggga gaagagaatt caggaggacg tacgcgacca gcattgcttt
    12841 gcgacctatt cgaagctttt ttaggcgcgt tatatcttga ccagggattc gacacgaccc
    12901 atgcattcat cgaaaaagtg atttttccta aagtcaaagc aggtgctttt tcacatgaga
    12961 tggatcataa aacaaaacta caagaagtct tgcaaaaatc aggtgatgta tcaatcgaat
    13021 accgattgat caatgaagaa ggtccagctc atgaacgagt attttggatc gaagtttatg
    13081 tagatgatca attgattggc actggacaag gaaaatcgaa aaaattagca gaacaagctg
    13141 cggcggaaaa tgcattggca gcactgtaaa agtaagtgag ggaaagcttt gtatttgaaa
    13201 cgaattgaaa tagccggttt taaatcattt gcagacaaga cagtcattga ctttgaaaat
    13261 agcgtcacag cagtggttgg accaaatggc agtggaaaaa gcaatattac agaagctatc
    13321 cgctgggtat taggagaaca atctgctaaa agtctgcgag gcggaaaaat gccggatatc
    13381 atctttgccg gttccgatac ccgtaagcag ctgaatattg cagaagtcac agtgatttta
    13441 gacaatagtg atcattatct gccattggag tacaatgaga tcagtgtaac ccgccgttat
    13501 cgacggactg gggaaagcga gtttttcatc aataaacaaa gctgcagatt aaaagatatc
    13561 caagaattgt tcttagactc tggattagga aaagaatcgt tctcaatcat ttctcaagga
    13621 aaagtagaag cgattttcag tagtaaaccg gaagaccgtc gtgggatctt tgaagaagca
    13681 gctggtgtct tgaaatacaa gcagcggaaa aagaaagcag aacagaagct ttttgaaaca
    13741 gaagacaacc tcagccgcgt ccaagacatc atccacgaat tggaagaaca gttaactcct
    13801 ttggccgccc agagtgaggc agcaaaggag tttttacggc taaaagaaac actgactcaa
    13861 accgatgtct ctttgatggt tgccgaaatc aagacagcaa aaaaagattg ggacaacaaa
    13921 caagcacagc ttgcgaaatt caatctagaa ttaggcaaac tatctgagtc gatccaagaa
    13981 caagaatcca ttttggcgaa gcagcgtaaa gaaaatgcgc aagccgatcg tctgatcgaa
    14041 aaaaatcagc aagtattatt agatctttcg gaaaaactga agcagacaga aggacaaaaa
    14101 gatgttttgc aggagcggac aaagcataca caaaaaagct cgcaagaata tcaaacttca
    14161 ttagcagaag cacaaaaaaa ggttaaacat tttgaaaagc ttcaagaaag cttgttgaaa
    14221 gaagtagcag aaaaagaaac agaaatccaa aaagctgaag caagtctcat aaaaacacag
    14281 caagaattag aaaaatacca aaaatcaacg aaagaattac tggcagaact aagagatcaa
    14341 tatgttgatt tgatgcaaga gcaagcagct gtaggaaacg aactgaagta tctagaacgt
    14401 caatatgtac aagaaacagc aaaaagtaag cagacgttag ccaagcaatc ggaagtagag
    14461 gcatcagtag accgattgat cttacaaaaa gaagaattga cacaaaagca ggcgcagcta
    14521 aaatcatctt taactgaaac aaaagagaag ttagaaatga tccaacaaaa cgggaaaaag
    14581 ttccaagaag cattagcaaa agaacagcct aaaatgtatc aattaatgaa tcaagtccag
    14641 cagctacgtg ctcgtaagaa aagccttcaa gaaatccaag aaaactattt tggtttttac
    14701 caaggggttc gattggtctt gcagcataaa cagcagcttt cgggtattgt cggagcagtg
    14761 gctgaactga tcgatgttcc tgcagatttc acgttagcta tcgaaacagc tttaggtggg
    14821 gcagcccagc atgtcatcgt tgaaaatgaa aaagatgctc ggcaagccat cacgtatttg
    14881 aaacagcaaa gaggaggaag agcaacattc cttccactga cgacaatcaa accaagacaa
    14941 ttaccggctc atatattgac acaagcagca gctgtagaag ggtttatagg gattgccagt
    15001 gaacaagtca gttatcccga tcaaatccaa actgtggttc aaaacctgct aggaacgatc
    15061 cttttagcca aagacttaac tagtgccaat gcaatcgcgc aaacgatccg ctatcaatat
    15121 cgagtggttt ccttagaagg tgatgtgatg aatgccggag gatccatgac aggtggagcg
    15181 aataaacgag gaaaccaagg cagtttgttt gtacaaaatc aagaattgaa acagttgact
    15241 agtgaatttg aagaagcaga taaacaactc caagcacaag aaaaaaaagt tcaggagctg
    15301 caacaagaaa cagctcgttt agccgaagaa caagaagttt tgcgtacacg aggcgaacaa
    15361 ttacgttttg aagaacaaga agcgacaaat cagctgcaaa acatcataaa tgaacttgaa
    15421 cgttttgaaa aagaaaagca aatctcaact tttgagacac gtgaactgca gcagtttatc
    15481 gaagattacg aaaagcaaac gaacgaattg aaagacaaac aaacagatct tgaatcgcaa
    15541 cgacaaaaaa tcgacgaaga aatcaaatcg ttaagtcaag aaagcgacca gatggaagcc
    15601 agaagagcac aagtacaaag tcaaaaagca caagaacaag cagatcttgc tgtcttgaaa
    15661 gagcagttta atcatctgca gatccagctc cgcggtgccc gagtccaaaa agcagaggca
    15721 accgaacgtc aagaagcgat agaaaagcag ttggcgacat tgacagcaga cttttctgat
    15781 catgaagtca cagaagaaag tttggaaaaa caaattaacg aattatctgc tcagcgagaa
    15841 acattgaaag ccgagttggc aaaagccaaa gagcagcgag atagaaggca aaaggagatc
    15901 gataagcttg aagcagtctt ggcagagcgt aatcgtgagc aaaaagcacg actgtcggaa
    15961 caatccaaat tagaagtcca aaaagaccga gctgaaatga ttttagataa tcatttgacc
    16021 tatctgcagt cagaatatca gctgacattt gagaaagctt cccaagacta tcaagaaaca
    16081 accgatatcg aagacagtag aacaaaggtt tcatccttaa aagaacagat tgaaaaactg
    16141 ggaccagtaa acttaaattc aatcgaacaa tacgaacaag tcagtgaaag acatacattc
    16201 ttagcaacac agcgagatga tctgttagca gcgaaaaacc aattgtttga aacgatggat
    16261 gagatggatg atgaagtgcg gacaagattc aaagaagtat ttgaagctat ccgtcaagaa
    16321 ttcaaaattg tctttccaaa tatgtttggc ggcggtaaag ctgaattagt actgacagaa
    16381 ccaactgatt tgctgaaaac aggtattgag attgaagtac aacctcctgg caaaaaactg
    16441 caaagtttaa gtttgctttc tggaggagaa cgagcactta cagccatcgc gttgctcttt
    16501 tctattatcc gtgtctgtcc tgttccgttt tgtatcttag atgaggtaga agcagcgtta
    16561 gatgaagcca acgtgaaacg ttttggtcgt tacttgagcg atttccaaga tgacacacaa
    16621 tttatcgtag tcacacaccg caaaggaacg atggtcgcag cagatgtact gtacggagtg
    16681 acgatgcagg aatccggtgt atcaaaaatc gtttctgtac ggatggaaga tatcaatgaa
    16741 gaggcgaaaa tcaacgaaac gaaagtgtag gaacgaaaat gatcaaatta gtagcaatcg
    16801 atttggacgg tacattatta aatgaacaaa agctgatttc agatgagaat aaacaagcat
    16861 tagcacaagc aaaacagcaa ggggtcaaga tcgttctatg tacaggacgc cccttagctg
    16921 cgatggtgca ttatctgcaa gaattaggac tggtagatga aggtgactac agcatcactt
    16981 tcaacggcgg tttggtacaa aaaaatgata ctggtgaaat catggaaaag aaagtgatgg
    17041 aagtatcaga tatccaacgc ttatacaaac tggctcagca attggatctg ccactagatg
    17101 ttttgtctga caatgtggtt ttacagctgc ctagtgcccc aaggaagcaa tccttgtaca
    17161 atgtgttgaa taagctgctg caattccaac cagcggatat aagcgatatt acagatgaat
    17221 ttgtgcttaa taaggcagta atcgcttatg accaagaaga attagatcct aaaatcaaag
    17281 aaataccagc agaattccat gaaatgtatg agatcatcaa aacgagaagt atgcttttgg
    17341 aatttatgcc aaaaggtgtt acaaaagctt atggtatttc tttattggca aaagatcttg
    17401 gtttgaaaca agaagaaatc atggcaatcg gtgatgaaga aaatgattta ccgatgatcc
    17461 aatacgctgg actaggcgta gcgatggaga atgctgtgcc tttcgtcaaa gaagcagcag
    17521 atttcgtgac agccagcaat gaagaaaatg gcgtagccaa agtgatccaa aaatttgtat
    17581 tagagtaggg gggagtcaaa tgggattttt tgataaaatc aagcgtgctt ttactggcga
    17641 agagaaaaca gaagaagttg aagtacagga gaagtacgac aagggtttag aaaagacgcg
    17701 taaaacattt ggtgaacgga tgaatgagtt gtttgccaat tttcgaacag tcgatgaaga
    17761 cttttttgaa gaattagaag aaacacttat cggtgcagat gtaggttttg aaacagcgat
    17821 acgaatcacc gaagcattac gtcaagaagt gaaactcaga aatgcgaaaa aatcggcaga
    17881 agtccaaaat gcgatcatcg agaaacttgt ggatctctat gaagcggaag gaatcaatga
    17941 agtaaatgaa ctaaatatcc aagaagacgg actaacagtt attttgtttg tcggtgttaa
    18001 tggtgtaggg aaaacaacaa gtattggtaa acttgcccat cagttcaaaa acgaaggtaa
    18061 aaaagtattg atggcagcag cagatacctt ccgcgctgga gcaatcgacc agttagttgt
    18121 ctggggcgaa cgcgctggtg ttgaagttgt tcgtggcaat gcaggcggcg atccagcagc
    18181 tgtcgtattc gatgctttgg aaagagcaaa gatagagcag gcagatgttt tactagttga
    18241 tactgcaggc cgactgcaaa ataaagtgaa cttgatgaac gaattagaaa aaataaaacg
    18301 agtaatccag cgggaattac cagaagcacc ccaagaaaca cttcttgtac tagatgcaac
    18361 aactggtcaa aacgcgatgg ttcaagcaaa acaattcaaa gaaacaacag atgtgaccgg
    18421 tcttgttttg acaaaattag atggtacagc aaagggcggt atcgtacttg ctatccgtaa
    18481 tgaattgcat ttaccagtga aattggtcgg tcttggtgaa ggaatcgatg acttggagcc
    18541 gtttgatcca aatgattttg tcgttggatt gttcaaagga ttactaaaag aagaataacc
    18601 catgtctaaa aagcaggagc tgtgacaaaa gttgcagctc cttgtttgaa tgattgtttt
    18661 taacatagct gtgtttttga tgccttcatt tttatactta tttgtaagcg gttctcaaac
    18721 aatttcgttc tttttcttag actttttgat tataattaga ttcatatgaa ttggaggtca
    18781 cttaatgatg aattttaagc aattattatt gcatgtcaat gcgcgccctt ttatcgacca
    18841 agctcgattt ggtatagaac gggaaggaca gcgggttgat cttgcaggaa atctagcaaa
    18901 aaccgatcat ccagcaatct ttggcgatcg atcctatcac ccctatatcc aaacagattt
    18961 tagcgaaaca caaacagaga tgatcactcc tgttaccgat tctattcccg aattatttca
    19021 gtatctcgct gctgtttatg atgtgactgc tcgttctata ccgaaagaag agatgatctg
    19081 gccattaagt atgccacctg ccttaccgga aaaagacgaa gcgatcatta ttgcaaaatt
    19141 aaaaaatttc gaagatgtct tgtatcgacg ttatttagca aaagaatacg ggaagcgaaa
    19201 acagatggta agcggtatcc atttcaattt tgaatttggc gatgagttgc taagaacatt
    19261 gttcagccat caggaagaat ttcaagattt ttccgaattc aaaacagaac tttatttgaa
    19321 aacggcgaga aactttatgc gctatcgctg gatgatcact tatttattcg gtgcttcacc
    19381 aatgagtgag aagaactact ttttagatga atcacatccg caagaacctg ttcgcagtat
    19441 tcgtaacagt gcgctgggct atacgaatca tccaaatgtg aaagtttcct atgcttctat
    19501 gaaacagtat ttagcagata tcgagcgaat gatcgaagaa gggaaacttt cggaggagaa
    19561 agaattttat acaccgcttc gtttccgagg cggaaagaaa gtcgcagatt tagcaacaac
    19621 aggtgttcgt tatattgaat tgcggaatat cgatttgaat ccttatgcaa gattgggtat
    19681 caatccagag caagttcggt tcttacaatt attcctgatg tacatgttat ggacagaaga
    19741 aaaagaagac tgtgaccaat gggtagcaga aggtacgact cgaaataaca aagtagcact
    19801 agaacaacca tcagatcaaa cagaatttca tcaagaaggt agagaaattc ttgaagggat
    19861 gaagcaaatg ctggttgaat tggattggtt ggattctctt tatttagtag aggaagcatt
    19921 gactcaaatg gatcatcctg aacaaacgtt agcagcgaaa ctctatcaag aagcacagct
    19981 atcgagtcag caagaagttg ctgtggcatt gggacatcaa tattataaag aaagccatga
    20041 acgtccctat cagttggccg gttttcgcga aatggaactt tctactcaga tatttatgtt
    20101 cgatgccatc caaaaaggtg tccaagtcaa agtattagat gagtcggatc aatttttgcg
    20161 tctgcaattc caagaccatg tagaatatgt gaaaaatgca aacatgacaa gtaaggacag
    20221 ctatatcgtc ccacttatca tggaaaacaa aacagtaacg aaaaaagtct taaaagaagc
    20281 tgggttccgg gtaccaggag gcgcagaatt ttcatccatg gaagaagcgg taaaagctta
    20341 tccgagattt gccgagcagg catttgtgat caaacctaaa tcaaccaatt acgggttagg
    20401 tatcacgatt ttcaaggaag gagctagtct ggaagattat caagcaggtt tagccattgc
    20461 ttttcgtgaa gacagttcgg ttttagtaga agaatttatg ccgggaacgg aatatcgttt
    20521 ctttgtgatc gacggagaag tccaagccat catgttgcga gtccctgcta atgttattgg
    20581 tgacggtatc cgaacagtga aagaacttgt tgaagaaaaa aatagtgacc ctttgcgtgg
    20641 aacgaatcat cgtgcacctt tagaattgat ccaattaggt gaattagaac aattgatgtt
    20701 gaaagaacaa gggttgacga tcgaatcagt tccccaagcc aatcaaattg tttatctaag
    20761 ggagaactca aatatcagta caggtggtga ttcgatcgat atgactgatg aattttctga
    20821 agcttacaaa aaaatcgccg tttctgcggt agaagcacta ggagctaaga ttagtggtat
    20881 cgatttgatt ataccagata aagaaatcga tcctacaact gataaaaaag catatgggat
    20941 catcgaagca aatttcaatc cagccatgca tatgcatgtt tatccctttg caggaaaagg
    21001 cagaagattg acgatgaatg tattgaaatt gttatatcct gaagtatttt aaaagaaaaa
    21061 gcttttgaca gattgtccta tttgatgtca atctgatcaa aagctttttt gcatgtaaaa
    21121 atttatgtgt tttttc
//
LOCUS       DAFBQH010000041        21114 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.041, whole genome shotgun sequence.
ACCESSION   DAFBQH010000041 DAFBQH010000000
VERSION     DAFBQH010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.041"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            474..725
                     /locus_tag="LK694_001663"
     CDS             474..725
                     /locus_tag="LK694_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052530.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            759..1037
                     /locus_tag="LK694_001664"
     CDS             759..1037
                     /locus_tag="LK694_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052531.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            1634..1891
                     /locus_tag="LK694_001665"
     CDS             1634..1891
                     /locus_tag="LK694_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052532.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            2004..2615
                     /locus_tag="LK694_001666"
     CDS             2004..2615
                     /locus_tag="LK694_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052533.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            2794..3351
                     /locus_tag="LK694_001667"
     CDS             2794..3351
                     /locus_tag="LK694_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052534.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(4050..4571)
                     /locus_tag="LK694_001668"
     CDS             complement(4050..4571)
                     /locus_tag="LK694_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBK5052535.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            4770..8462
                     /gene="nifJ"
                     /locus_tag="LK694_001669"
     CDS             4770..8462
                     /gene="nifJ"
                     /locus_tag="LK694_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBK5052536.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            8651..9538
                     /locus_tag="LK694_001670"
     CDS             8651..9538
                     /locus_tag="LK694_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBK5052537.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            9535..10704
                     /locus_tag="LK694_001671"
     CDS             9535..10704
                     /locus_tag="LK694_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052538.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            10728..12083
                     /locus_tag="LK694_001672"
     CDS             10728..12083
                     /locus_tag="LK694_001672"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBK5052539.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(12155..13960)
                     /gene="glmS"
                     /locus_tag="LK694_001673"
     CDS             complement(12155..13960)
                     /gene="glmS"
                     /locus_tag="LK694_001673"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBK5052540.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            14369..14659
                     /locus_tag="LK694_001674"
     CDS             14369..14659
                     /locus_tag="LK694_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052541.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            14672..15697
                     /gene="sppA"
                     /locus_tag="LK694_001675"
     CDS             14672..15697
                     /gene="sppA"
                     /locus_tag="LK694_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBK5052542.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            15700..16359
                     /locus_tag="LK694_001676"
     CDS             15700..16359
                     /locus_tag="LK694_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBK5052543.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            16516..17448
                     /locus_tag="LK694_001677"
     CDS             16516..17448
                     /locus_tag="LK694_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052544.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            17602..18891
                     /locus_tag="LK694_001678"
     CDS             17602..18891
                     /locus_tag="LK694_001678"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBK5052545.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            19138..20388
                     /gene="clpX"
                     /locus_tag="LK694_001679"
     CDS             19138..20388
                     /gene="clpX"
                     /locus_tag="LK694_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBK5052546.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            20471..21058
                     /locus_tag="LK694_001680"
     CDS             20471..21058
                     /locus_tag="LK694_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBK5052547.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT         7025 a         3605 c         4568 g         5916 t
ORIGIN      
        1 acaaataatt ggtttattct ggcaggcgta atgctttttt ctagtttcta tttatttagt
       61 aatgctctat tagcacagcg agggaaatat aagtttaaaa tattagatta gatattattt
      121 ttagagggga taaatataaa atgaaaagag gttatttata atggaaaaag tattaccaat
      181 taagagctac tgtattccaa ggcttttcat catagaattt caaaagtaaa gacatgtgtt
      241 tcacaaatag tgtctatcct ggctaaataa ttcttccact atctgcttta atttcagtag
      301 acttttcggc aagttatttg ctaatgagat gaatagagat tacaactcgc ctataaccgc
      361 ttaaaacaag cttttttaaa gtatagttag caagtcgtat tcatatgttc aaagaggagg
      421 gcttatgaac tttgaattca ttccgtgtga acagaacagt agggggaagt aaaatgaaag
      481 aaaagcctat aaataaatgg tcattgtata aaaattcaat gtttggagtt gttatctttg
      541 gtgtttgtgc tggattattc cttttcatat tattacaaac ggtgactctt ttctcttcta
      601 atcctactta tggatggaga gacagtatag ccttagggat gtttgcggga ggatatggat
      661 tcttattgtt gttctcaatt ctcacactaa gtccaatcca tggactcatt caactttgga
      721 agtaagaacg agccttaaac ttttcgttct cggaagaaat ggaaaaacac aacataacag
      781 gcagtatgta tgtggatgat caatggttta tcagcactga tgaggtaaaa atagctgcca
      841 ttaggagaga ttacctgcag aaaattcata aaataaaaga tcctaatggg aaaaaggcaa
      901 ggttaattat aactttgata acatctaatg gtaagaagtt aaaacttgca ggatcatatg
      961 ataatgttaa aaagttcgaa cattggtatc tgcaaaaaga cagcattagt cttaataaac
     1021 aatttgaaaa aaattaatca aagtcatcgg caacacgaat aatttgacat cttgatattg
     1081 tgatataagc aaaaacggca tttctgagac cgaacagcat aaaaaattta aatgttggaa
     1141 tttatataag caaaacccaa gaagctagat gcgatagagc atttaatttc ttggggtttt
     1201 gcttgggaat cagcatatca attaaaatga tcatctcggt atttcaaaag tgggtacgat
     1261 tacaaatagt tggagcaaaa tagtgggtaa aattaccatt ccagtgtctg tcttgcctct
     1321 ttttcgtcaa tcacaaatag acgagattca taggaaaaat cgatacaggg tatgatttca
     1381 atgatattat gaccaattac aaaatagatt gataaagcat tttttgcatt tttataatag
     1441 tttattgata ataatgtatg agatacagta taatgattta aaaggtaacg tgttgtgacc
     1501 gaattaaatg aattaatgcc tattttttat attccttagt ggacaaaaat ggagacagtg
     1561 aagttaggag cattgtatcc tttgttgcgt agttcaaaaa agtaagcgat ttctataatg
     1621 gtcagggtaa acggtgaggg ctgtccacgt aaatcctact attgaaatca caatgatcgg
     1681 acattcaaaa ggtggtgctg aagcagcaaa ttcagtagct ataaatacga atgcgattat
     1741 atttaatcca gcgacagtaa atttatctgc ttatgaatta gactcaaaca catatacagc
     1801 aaacatggta gcttatgttg tcgagggaga tgcattaaat gttgtttttg gaggagtata
     1861 cacctattgg agaattaatc atgttagata gacaatatga tgcaccttag tggagaatga
     1921 tatctcgcat aggtaactca gtaaaaaatc aaggtatgga agcaattatc tctgcattgg
     1981 aagatcgata tagataggcg gatatgaata gtaatagggg gaataaaatg gaatacaatg
     2041 gatcaatgag aattgtacaa attatatttt ggattacatc gggaattgtg atcataggag
     2101 gagttttttt gatgttgcca agcttaatat ttcctttctt tgcattaatc tcacctaaaa
     2161 tacccgaacc ggaaatcacc tatggagaat ttccatttag gattgaatac gaactagatg
     2221 gacagattaa cataattgaa gatactgtca tagctgagtt taacgggtgt gagttcagcg
     2281 caggttctat gaaaagagaa cgaagatgga gaagtagatt agctagtggt agagaagatc
     2341 taccttttgg ggacgatcta ggaatctatt ttagtcgagg aagtgcgcaa tattatatgg
     2401 gggaaaatgt acaatcaatg tcattaaaac cacatatagc ccttagaaat ctcgaggaag
     2461 gttttaaaag agaggtagac tttatattag gagaaagtgg atacataaga actgtactta
     2521 atttcggaga agcgcaagaa gttttgacac aatacggaat taccttaatc aactgggaaa
     2581 tatctgaacc gattgttaac aattttggtg attagaaaac aattacgata aatagaaaca
     2641 agccaaatca tcagcaagta aaagctgatg atttggcctt tcttatacga gtagtaggaa
     2701 ttcatagcga atattattac gaccagtaga tattttgata ttaaaggagt attacgaact
     2761 aatatgtgag aatgtagagg tgatgtgcga ataatgagaa atttatcaaa tagaaacaaa
     2821 atattaatca ttattgttgt tatagctgtg tttcatctgg gaacgaatgc ggttttaagt
     2881 agaataatat tgggtcctaa gccaccaaga ccagaaatta cccgagggga gtttgatttt
     2941 agattagaat atgaagtaga tggggaaagg attgtgatag aagatacaat tgttgcatta
     3001 tttgatggat tttctgcaga tgcaggtagc atggcatggt atcgcacgtg gcgtctacat
     3061 ttagcaagtg atagacgatc acgaaatatc ttattagatg aattagagga tggtcgaaga
     3121 atatattatg tcccagaaag tgctaactat tctatgggag atgtacagaa aaaaagagag
     3181 cctaatccta attggtatcc atttaatggc gtaacaatag aatatccacg caataaaaca
     3241 cctgaaatag gtgcaaaatt tataagtggt ttagaagatc tgtacgatag gtttggaatc
     3301 cgtcttatta gttgggaaca cgatccacca atagaaaatc gctttgaata atcgaaatct
     3361 actaatccat ttcatcagcc agtgaatgtt gtaatggaac aatttggttt agtgttcaaa
     3421 accattgaaa tactaggctg tgatcagcaa aaatgaatct gaataaaaaa gagagcaact
     3481 attagctgag aggagttaat agttgttagc tttatacgaa tcaaagtggc aaatacagat
     3541 gcgtatgcat ttgtgtttgc catcttgcgt ttggtagacg agataggtat aagctaaaga
     3601 aacaacttgt ttaaaataaa cgacctagct caacaaatga ccatctaatc aactaacagc
     3661 atacataaaa gcaactattt ttgaagtaga attgaaatcg ggataactta aaaagtgatt
     3721 gggatactag tgagaatcac cgtacataaa tattactcgt taagggaaat tgagaaattt
     3781 tgtatgaaaa tttaagaatt agttagtatg cgcaagtcaa aaatatgaat aaattatttg
     3841 gtggagataa tttgcttagc ttgtcataag aaatgagata atctggcaga tgcaagcttt
     3901 tggacaccat ttattcggaa taaggaggct gggacagaag cgtttaactc caagaaataa
     3961 gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc
     4021 tttacttttg tcccacgctc cttatttttt tattttgttt ggatcaattc ttttaattcc
     4081 tctattgaaa tagattcatc aaagatttct ttacccaaga tgacagttgg aacaaatcga
     4141 atatgtgctt gctcagcttc gttgatgaca gcttggctca gctgttcgtc tttttgttct
     4201 gtcagcttta gtttatctac tgcaaaatcc gctacttctt ggagagaaag atgtttccac
     4261 tcgtcttgag tatcgaatat ttgtttgatc tctttgatcg ctttttggcc atcgctgatt
     4321 gtgagatacc ggtgcatcac gttccctcgc tgtaagcttt ctttttcttt atcgaacaat
     4381 ttgatcactc gctgcaattg cccgctttgg actgcttctt ctaatagctc ataagattct
     4441 tcaaaccatt gtcggcaata cggacaagct aagttgatga attcgaccat ttttttagga
     4501 gcattcgatg aaccataaag gattcctttt tgtgtatttg ttttcgttgc atcaatcact
     4561 gatatatcca tgttaaaaca cctctttcgc ttatagttta tacatgtctg acaaaaaaag
     4621 gcaacgattt agactgtttg tctaaaaatt agatgaccaa ttcaaataga tacatgtaaa
     4681 atatttttat aaataaaaga aaaaaatggc atgttttacg ttataatgga acagataata
     4741 agagagaata tgaacaagga tgatgaatca tgcgaaaaat gaaaacaatg gatggcaata
     4801 cggcagccgc atatatttct tatgcattta ccgagattgc tgcaatatat ccaataacac
     4861 ccagttcgac catggctgaa ttagtagatc aatggtcagc agcaggaaaa gaaaacgtat
     4921 tcggtcaacc agtaaaagta gtagaaatgc aatccgaagc aggagcagct ggtgtggtcc
     4981 atggatcatt gaaaactggt gcactgacaa ctacatatac cgcttcacaa gggctgcttt
     5041 tgatgatacc gaatatgtac aagattgctg gggaactact gccaagtgtt ttccacgttg
     5101 ctagtcgggc agtgacgaca aatgcgttga atattttcgg agatcacgga gacgtgatgg
     5161 ctgcaagaca aacaggattt gctatgttag ctgaaagcag tgtacaagaa gtgatggatt
     5221 tagcacctgt tgcccatctt gcttctattg aaggagaagt cccttttttg aatttctttg
     5281 atggattccg tacgagtcac gagattcaaa aaatcgaagt tttggaatat gaagaattag
     5341 ctcctctgat caatcaggag aaattagctt catttcgtaa acgagggatg aatcccaatc
     5401 atccgactgt tagtggtacg aaccagaatc cagacatcca ttttcaacaa cgggaaacga
     5461 tcaaccccta ttatgataaa ttacctgaaa tcgttcaaaa atatatgaaa gagatcaacc
     5521 gattacgagg aacttcttat gacttggtga cttattacgg agcagacgat gcggaagaag
     5581 tcattgtttc tatgggatca gttgcacaga cgattgaaca aacgattgat tacttgaatg
     5641 caaacggacg gaaaacaggt tttttgaata tccatcttta tcgtcctttt ccagtggaaa
     5701 cattcgtgca aaagttgcct catacagtaa aaacaattgc tgttcttgat cgaagtaaag
     5761 aaccaggggc aggtggagaa cctctcttat tagacgttca aagtgcgtta tatgattctg
     5821 aacttcgccc ggctgttatt ggtggtcgct acggattggg gtctaaagat gtgactcccg
     5881 accaaatctc agcagttttt gatgaattga aaaaagaccc aagcatacgc aaaaaacggt
     5941 ttaccatagg gatcgtggat gatgtgactt atcaatcttt ggaaccaaag gaaagtctgg
     6001 atttgacaga accgcaaaca ttccaggcca agttttgggg atttggatct gacggaacag
     6061 ttggagcaaa taaatcagct atcaagatta ttggagatca caccgataaa tatgcgcaag
     6121 gctattttta ttatgattcg aaaaaatctg gcggtctgac tgtctctcat cttcgttttg
     6181 gagatacgcc gatacgttca gcgtatcttg tcgagcatgc ggaccttgtt gcctgtcaca
     6241 caccagctta cctgcatagc tatgacttgg tcaaaggatt gaagccaggt ggcatttttt
     6301 tattgaatac tctttggagc gacgaacaat tagaaacgca tctgccctta aaattaaaac
     6361 gttacttggc tgaaaataac attagattct atacgatcaa tgccatgaga ttggcccaag
     6421 aagtcggttt gggacgccgg atcaatacgg caatggaaac ggcttttttt aaattagctg
     6481 acatcattcc ttttgatgag gtgcttccgc tgctaaaaga agaagccctg aaaagctatg
     6541 gacataaatc aatggctatc gtagaaaaaa acatacaagc aatcgatcgg acgaccgaac
     6601 tgcttcatca agtcgcgatc ccagaccatt ggagaacgtt ggagatgcct gaaaaaatca
     6661 ataaaaggaa cactggttat atacaagaaa tacttgaacc agtgaatgca caggaaggaa
     6721 atcaactctc tgttggtaca cttgtagcaa atggaatgac aactggcgaa atgcctttag
     6781 gaacggcttc tatggaaaag cggggcgtcg ctttagaagt acctgagtgg attagtgatc
     6841 gctgtacgat gtgtaatgaa tgtgcctttg tttgtcctca tgctgccatc cgtccatttt
     6901 tagcagatga agaggaaatg gaagaagcgc cagaaggatt tatcgtgaga gaaatgcgag
     6961 gcgcagacgg cgtaaaatac cgtatccaag tttcagtgga agactgtaca ggttgtggtc
     7021 tgtgtgtaga ggcttgtccc gcaaaagaaa aagctttagt gatgaagcct tatgaagaac
     7081 agaaagaaca agctgtgaat tgggcttttg caatgacatt aagacaaaaa gcaaatccag
     7141 tcaaaggaaa gaatactgtc ttgtctacac aattcaatca accgcttctt gaattctcgg
     7201 gcgcttgctc aggctgtggc gaaacacctt atgtaaaatt attgacacaa atgttcggtg
     7261 atcgaatgat gatcgcgaat gcgacaggct gttcttctat ttggggcggt gcttctcctg
     7321 cttctcctta tacgaccaat gaatgtggtc aagggcctgc atggtcaaat tctttattgg
     7381 aagataatgc tgaatttggt tatggtatgc ttctagcggc acagactcgt cgcgaggctt
     7441 tggcaagttc aatcaaagaa gtattgccaa aagtatcaga agaactacga gaattgatgg
     7501 aagactggtt agtacacatg tttgagagtg aaggaacgca gcaaagagca gcaaaactgc
     7561 aagctgcgat cgaaagcgaa cgatcaattt tccctgaact ggaggatata tacaagagaa
     7621 aggatctatt cgtaaaaaac agccaatgga tgattggtgg agacggatgg gcttacgaca
     7681 ttggttttgg cgggatcgat catgtacttg ccagcggagc agacgtgaat atccttgttt
     7741 tggataacga agtctattcc aatactggcg gtcaaacctc aaaagcaaca ccagcttcgg
     7801 caatcgctaa attttcggct agtggaaagt atgcttcaaa gaaagatcta ggaatgatgg
     7861 cgatgacata tggaaatgtt tatgtggcgc aaattgcttc aggtgccaat caaatgcaaa
     7921 cgatcaaggc atttgaagaa gcagaaaagt atcctggtcc ttctctgatt attgcctata
     7981 cgccttgtat cacacatggc ttgcttggcg gaatgaaaga atcgcttaaa gaagcaaaag
     8041 aagctgtttc ttctggttat tggtcattat atcgatataa ccctacaatg gtcgaaaaaa
     8101 acaagaaccc aatgactttg gactacaaaa aacctgattt ctctgctatg cctgatttca
     8161 tgaggaaaca agttcgcttt tcctcgctag ataacgcaaa tccaaatctt gcaaaagctt
     8221 tatttgaaaa gactgtccac gacgctaaaa cacgttttta caactatgct gcactgacgg
     8281 gagaattaga aaaaatcaag aaacgacttg aaccagagga atctatcgat gtacccgtta
     8341 agactagaaa agaaaaaacg acggaccttg aagcagaaga aagaagagct gctagaagag
     8401 ctgccagagc agaacgacgt aaaaaaatgg aacaagctac aaaagaaaaa aaggaaaaat
     8461 aacagaaaaa atataatgaa taattctctg aagtttaaaa atatttattg cagagaaaaa
     8521 taaaacgcat acttgcaatt ttcttgaaaa ttatgcgttt ttctcaagaa aaatacgagc
     8581 atatcagcct ctaaaattaa aacatatgat atactagaga ggaaatctca aaagagaaga
     8641 gaggcttttt atgtcatttc aaatcgggga actttttaat ttggactatt ggcagcaaat
     8701 gttttccggt gacttgttct ctctgaattt tttggtgaac atacttgata ttctggttgt
     8761 ctggtatctt gtatacaaac tgatccaact gctgcgaggc acaaaagcga ttcagcttct
     8821 gaaaggtgta gctattttta ttgttatccg aatcgtagcg gaaatcattg gtctgcacac
     8881 cttgtcttgg ctgatgaacc aagtcattac gtacggggtg atagctgcta tcgtgatttt
     8941 tcagccggaa atccggagag gactggaaca tcttggacgc agttcgcttt tcaaacaatc
     9001 aaaaagcgaa cagcaggaag atgagaagat gatcttgtct tttgacaaag ccatccaata
     9061 tatgtccaag cgaaaaatcg gtgcgttgat cactatcgaa cgtcatactg gattagatga
     9121 gtatattgag acagggattg ctttagatgc agatatcact ggggagctgc tgatcaatat
     9181 ctttattcct aatacaccgc tgcatgacgg tgcagttatt gtaaaagaag gaaagatcgc
     9241 agtagcaagc gcctatcttc ctctatcaga aagtatgttg atccctaaag aattcgggac
     9301 tcgacaccgg gcagctgtcg gtataagcga agtcagtgac gcgattacca tcgttgtctc
     9361 tgaagaaacc ggggacgtca gcatcacgtt agataatgaa ctgatggctg gcttatccca
     9421 gcaagaatat ttggcaatct tgcgtagaga actaattgct gaagagaaaa caaaaaacaa
     9481 gaaaaacgtg attcaatctt tcctagacgg tatgactaag ggaggggatc gcaaatgatc
     9541 tcaaaagaga aacggacaag cattctttac agttttattg cattgctgtt cagtattgcg
     9601 ctgtacttca atgcaaatgg ccagtccgtt ccaaatacgc tttctggaaa tgaagcgtat
     9661 aatcaaacag ttacgggggt gccgatcaag atttcttatg attcaaagcg ctactatatc
     9721 catggatatg aaaatgcagt gactgtcaaa ctttccagtg caaaccgagt tcagttgaat
     9781 gcagaggcaa acgaagatac acggatgttc cgcgtcacag cagatatcac taagctgggc
     9841 gaaggaacac atgaagttcc gctaaaaatt caaaatttga gcagtgctgt cacagcaaag
     9901 atcgaaccat cgactattac agtaacggtt gagaaaaagg taataaaaga tttcgatgtg
     9961 gaaacgttga ttccttcaac gttcacacca tctggatatg aacttgatga tacttccgtt
    10021 agtcctaaaa aagtctctat cactacaggt gacaaaacat tggcagaaat caaacgagta
    10081 gtagctgatg tggacgcttc gatggtcaca gatgatggga ttaatagtga ggtcccaata
    10141 caggcgctga atgcagcagg ggaagtatta agtatcgtct ctgatccagt gcaagtaaca
    10201 gtgagagctg attcgatcaa gccaagcaag tcggtacgac tttatgggat acagcaagga
    10261 acaccagctg ccggggtaga aagctatgat ttcagttttt caaaactgga agcagaagta
    10321 agcggctcta gcgatttgct tgcatcgatc ggtgacagta ttgctgtacc gatcaatgtc
    10381 tcaggtatca atcatcgaac aactcgtaaa attgaaatac cagtagaaga ggggttaagt
    10441 atcaagcccg aatcagttag tgtggagatt acacccgtgt tgcaaacgtc aagcagtagc
    10501 tctgcttcga cgagcagtcg gacacaagaa aatacaagta cgagtagaag tatttcatct
    10561 gctgattcag gaacggaacc atcgacaaca accatttctt ccagttcgga atctgggaca
    10621 agcagctcga ccacaagtac agaagaaaca tcagaaacaa gcgaatcaac acaaatgagc
    10681 agaacaaatt cctcagaaaa ttaagttaaa ggagtaattt tacaactatg ggtaaatatt
    10741 ttggaacaga cggcgtacgc ggtgaagcca acaaggaact tacgccagaa cttgcattta
    10801 agctgggacg gtgcggaggt tatgtcctaa gccagcatga agaagaagga aaacgcccac
    10861 gcgtgttagt agggcgggat acaagaatct caggtcaaat gttagaagaa gcgctgattt
    10921 caggactttt atcggtgggt atcgaagtgt tccagctagg tgtgatctct acaccaggtg
    10981 ttgcttatct gacacgtttg caaaaagcaa gcgcaggggt catgatttct gcttctcata
    11041 atccagcaca agataatgga atcaaatttt tcggtgcaga tggttttaaa ctagtagatg
    11101 accaagagct agaaattgaa gcattattag atgctgaagt ggatgaattg cctcgtccat
    11161 cagctgaagg attgggaact gtagaagaat ttccagaagg cttattgaaa tattctcaat
    11221 tccttgtaca aacgatcaac ggtgaccttt ccggtttgac catttgtgtc gatgcagcaa
    11281 acggagcaac ttcaacaagt gttaatcgtt tgtttgccga tcttgaaaca gatttttata
    11341 caatgggcac aaatccagac gggttaaata tcaatgacgg cgtaggctct acccatccag
    11401 aacgtttagc tgagatggtc gttgaaaaag gagcagatgc tggactagct ttcgatggcg
    11461 atggcgatcg tattattgcg gtggatgaac ttggaaacat tgtcgacggt gataaaatca
    11521 tgtttatctg tgctaaatac ttggcagaaa aaaatcgatt gaaaaaagat acgatcgtta
    11581 cgactgtcat gagtaactta ggattccaca aagcggtgga aggaatcggt ttgaaagatg
    11641 tcgttacaca agttggtgat cgttacgttg tagaagaaat gcgcaaaaac gattataact
    11701 ttggcggcga gcagtctggg cacatgattt tcttagattt taacacaact ggagatggga
    11761 tgctttcagg aattcagtta ctgaatatca tgaaacagac aggtaagaaa ttatcagagc
    11821 ttgcagcaga ggtaacaatc tatccgcaaa aattagtgaa tatccgcgta agtgataaaa
    11881 acggtgcgat ggatgttccg gcaatcaaaa aagtgattga agaggcagaa gctgaaatgg
    11941 gatcagaagg aagaatccta gttcgtcctt ccggtacaga accattactt cgagtgatgg
    12001 cagaagcgcc tactgatgag aaagttaatt actatgtcga taaaatcgcg gcagtagtca
    12061 aagaagaaat cggactggat taagagatat tttcataaaa aataaaagaa caaggaccag
    12121 tgtcagatgt ctgacactgg tccttgttct tttattattc tactgtcact gatttcgcta
    12181 aattacgtgg tttgtcaacg tcgaatcccc gtaatagcgt tgcgtaatat gcaagtaatt
    12241 gagtcggtac gactgtggtt agagtagtta gataagggtg gacagttggt agcaccagct
    12301 ggtcactttc cttttctaat ccctctgaga caataactag tgttttagcg ccacggcttt
    12361 cgacttcttt caggtttcct cgagtatgcg caccagtcac ttcttcactg atgattccga
    12421 tcactggtgt tccttcttcg atcaaagcaa tcgttccatg tttcaattcg ccagcggcaa
    12481 aaccttctgc ctggatataa ctgatttctt tcaatttcaa agcagcttca agagaaacgt
    12541 tgtagtcagc accacgtcca atgtaaaagg catttcttgt atcgctgaga tatacttctg
    12601 ccaactcttc taaataatct ttttgggcga tgaccgattc catcgcatta gcaactaaac
    12661 tcaattcatg agtgatatcg aatacaagag aagtttcaaa ttctttttta tcaccgatag
    12721 ctttggctaa taaagttagt acagctattt gtgcagtata agctttcgtt gatgctactg
    12781 cgatttcggg tccagcatgc aatagcagtg taaaggatgc ttcccgcgaa agagtagaac
    12841 cagcaacatt tgtaatcgta agtgaagggt agccgagacg gttgattttt accagcactt
    12901 ggcggctgtc tgctgtttcg ccactttgac tcaagaaaat aaagaatggt ttttttgaaa
    12961 gaagaggtgt gttatagcca aattcactgg ctaaatgaac ctcaacagga atctgtgtca
    13021 atttttccaa agtctgttta cctgctaacc ccgcatggta actagttccg caagcaacga
    13081 tatagattcg atcgctatca gacaatgtat cgaccagctt ttgatcaaga gagacacgat
    13141 tgtcttctgt tacgtatttt tgagcgatac gacgcatgat aataggctgt tcgtcgattt
    13201 ctttcagcat ataaaaagga taggtacctt tctcgatatc tgaagcatct acttgtgctt
    13261 cgaatgaaga acgttctacg atgtcacctg caaaattttg aataataact ttttctgatg
    13321 tgacggtaac catctcttca tcttcaatct caacgaattc cttcgtatga gcaagcatag
    13381 ccattgcatc gctggcaatc acattgaatc catcgccgag tccgattaat agtggacttt
    13441 tatttttagc gacgtaaata acatcaggtg ctgtccgatc aatcaaagcg aaagcgtaag
    13501 aacctttgat gacccgcaaa gcttttaaaa acgcctcttg cgttgtagta tccttcgcga
    13561 aagtttcaat tagatgagcg ataatctcag tatcggtctc accaataaag tggtcatttg
    13621 ctaaataagc aattttaagt tcttcaaagt tttcgattac gccgttgtga acgagcacga
    13681 agcgtccact ttgggaagta tgtggatggg cgttttcttc agaaggttgc ccgtgagttg
    13741 cccatcgtgt atgaccaata cctatatttc ctgtagtatc aatatttatt ttgtttttca
    13801 aattttgaat tctgccttgt gctttgacca gataatcagt tgtgccatct gagacaaaga
    13861 taccagctga atcataacca cggtattcta atttttctag accgtcaatc aatcctggtg
    13921 ttacattttt taatccaatc attccaacaa ttccgcacat aaaaaacatc ctactttcta
    13981 taatgaattc gtatgaatgc ttccagctgc aggaaaatcc ttttgtttta tctttgcaga
    14041 cattttttgt ggattttctg tagagtgcag tccctagggc catccgccga atctttcgat
    14101 aaaccctctc ctcgtcatct gtttacacag acctggcgct atcctaaaag gtataactat
    14161 cctccttttt gttttttatc tggaaacatt taaaatcata accttttgcg aatgttcttg
    14221 tcaacaaaaa aagaataaaa aataaattgg tatatatcaa attctttgtc aaatgtcctt
    14281 tttatgtcaa aaaataatgg gtttttatct atcatctttt tcaggagttc ggtacaatag
    14341 aaagtaatcg gatagaggag gagtaaaaat ggattcaaaa attgagatca tgacactggg
    14401 aatgttaaaa aaacaattgt ccaaattcga agcatcagct ggtgtatcag atgatacgaa
    14461 aatattttta gatactggct gggatagcat ccaagaaatc gcacctgatg ccttggaagt
    14521 tgctcaagct cgtgagttta cggtagaaga tgaattaaca aaagagtcgt tttccgggta
    14581 tgcgcgggaa gaaaaagcag agcggttcga cacttctgag cagtcagaaa ctgtgatcgt
    14641 cataaaaaac ttgtattaga aaggagaaag aatgaataaa agacgttggg cagcagttct
    14701 gattgctgca ggattatttg tggtttcgtt agtttcagct agtttgacta agcctcagga
    14761 aaacgatcaa attgagggat tgaacagttg gttatatggg gatgaagaac tagctccaaa
    14821 tgtgttggaa gagggagaca gtacgcaaaa aatcgtaaaa ttatcagtag atggaacgat
    14881 tgctgcggga gggtcttcag gtttatttgc ttctgaaggt tacaatcata aaaattttat
    14941 ggaacaactg aaggcgattg aagaagatcc aacagtcaaa ggtatctttt tagaagtaaa
    15001 ttctcctggt ggcggtgtct acgaaagtgc ggaaatcgct aagaaattgg atacgattcg
    15061 taaagaacat gatatcccaa tgtatgtcag catgaaaaac atggcagcta gcggtggcta
    15121 ctatatttct gctcaagcag ataaaatctt tgcaacagaa gaaacagtga ccggctcaat
    15181 tggtgtgatc atgtcaggat tgaattattc aggacttcta gaaaaactgg gtgtggaaga
    15241 tacaacagtt aaaagcggag cgttgaaaga tatgggttca ggaacgcgac cagagacaaa
    15301 agaagaagaa gcggtgcttc aagcatatat cgacagcgca tatagtcgtt ttgtagcaat
    15361 cgtcagcgag ggacggaata aatctgaaga agaagtcaaa aaaattgctg atggaaggat
    15421 ttatgatggt gtccaagcaa aagaagtagg tttagtcgat gaattgggat ttcctgaaga
    15481 tgctttaaaa gctatacgaa aggaacaaca gcttgaagat gcggaactag tagaatattc
    15541 ttccacgcca tctgggtttg ctagtacatg gttaggaagt aaactagcag aattccaggg
    15601 attgaagcct tctgagacta gtcagatctt atccgttctt gagaatctag ggacagcgga
    15661 atcaccaaaa gcaatgtatt attacggagg tgagtaagca tgtcagagct ttcaaatgaa
    15721 aataaggaac aagaaaaaga taagcaacag cagcctgctg taggacctga ctccaacttt
    15781 tcccaaaagg tactgagctc ttttgaaaga gagcctttga cgcccgaaga aatcaaaaag
    15841 aaacaacgta aatggaaaaa gtatacggac acaagaaatg aaccgccaaa agcaaatgat
    15901 tttcctaagt atttctatgc aggttttttt atccgaatgt ttgccttctt ggtcgatctt
    15961 ttgtgtatcg gagctattac gcgtttaaca cttggtattg ccaaaaatct aggttggctt
    16021 aatttttcag attcttatct gagtctctac ggattcacag ccttacttat ttatctagcg
    16081 tattttattt tattgacgaa attcaatcat ggccaaacga ttgggaaaat gatctttggt
    16141 attcgagtca tcagctttga tgaagaagaa cttagctggt caaccgtttt agttagagaa
    16201 ggtgcttgcc ggttcattct aaaatttcct ttgctgatgt tagggtatct tccagcagtc
    16261 tttaatcaga aaaagcaaca tctgggcgat tttttcacta atacaagtgt tgttacgatc
    16321 aatctgatca aagcgttcaa ccaggaagtc aacagctgat gagcaataaa aagcagtaac
    16381 agttgaaagc accttctaaa agtcagatct aaaatctagc ttttagaggg tgcttcagtt
    16441 atttttaatt gtcatgcttt ttttgttttg ctactattga acatgagctg aatatgagca
    16501 agaggaggac actgcgtgaa gccgattgat tttccagaaa aatatgagaa tttaatgcga
    16561 gtggcacaac aagcactagc aaaccagcaa tatcagcagg caaaagaatt atttcaacga
    16621 gcttacgagt tgaaagaaag ctttgaggct aatagcctgc ttgttttttg tctttacgag
    16681 ttggacgaga aaaaagaagc attgaagcaa gcactcttgc atgaaaaaca gtatcttcaa
    16741 aatgaggagt ttgctgaatt ttattttgac ttgttaatca gtacacaaga ttttttatat
    16801 gctagaaaat tgatagcaag tacagatttt tatgaagctt tcgagcagcg ggtcattgaa
    16861 aaaatccaat ttgcagaaga attatccgga caaatggaga ggcagaagat caaggcactc
    16921 cacaataaat cggaggaact tccttcgttg gaacctgcac gtcagcttgc gtctattgat
    16981 gagatcgaac agcttcctta tcatgagttt atccaaacag cgagtaaact tattgtatcg
    17041 ccagaggtcc atattttagc aagggcaaaa ttactggaga cacttaggca attgaacgaa
    17101 tgccatcctg ttttctattt gacgattgaa gaaaagttag tcaaagtcat accaaaagat
    17161 ctgccaaagc cgcagcagca ggcaagttac cagcaattat gtgtattttc agatcgttac
    17221 gggaatgaag atgcgctgct tagttccgtg ttaaaagaag aattcacgtt gcaatctgca
    17281 attgtttatc ctgtctatga tacatacatc gaagacccga agcgctggtt tcaactgact
    17341 gtagaggcgt gtacaggaaa aacgatgtat gaaggtacag aagatgaaaa acaagatttt
    17401 ttaaaaaaaa gagaaaaaat tttgcagcaa atgatcttct tccattagat tttatcagtg
    17461 aaaaagataa aaatgttatg aaaaacaagg attctatcca ttcaatatgt ttacaatttg
    17521 aaagggttag tgtactattt aaaggtatgc atgaaacgtt tttgatgtat aaaatgaatt
    17581 tctaaactcg gagggaatta aatgactgca acatttgaaa aaaaaggcac gaatgatggt
    17641 gttttaaaat ttacgattgc gcaagacgaa atccaaaaag gattaacaac tgcgtttaat
    17701 aaagtgaaaa aaagcttgaa cgtacctgga ttccgtaaag ggaaagtttc tcgtcaagta
    17761 ttcaaccgta tgtacggtga agaagcttta tatgaagatg cattgaatgc tgtattacca
    17821 gaagcttatg aagctgctgt caaagaagct ggcatcgatc ctgtttctca accaaaaatc
    17881 gatgttgaaa gcatggaaaa aggtcaagac tgggtaatca ctgctgaagt aacagtgaaa
    17941 ccagaagtaa aattaggaca atataaagat ctagttgttg aaaaacaaga ccgtgaagta
    18001 acagatgctg acgtggatgc acgtatcaaa cgtgaacaag aagcacaagc tgaattagtt
    18061 atcaaagaag atgctgcagc tgaaaatggc gatacagtag taatcgactt cgaaggcttt
    18121 gtagatggcg aagcgtttga tggcggaaaa ggcgaaaatt actcacttga attaggttca
    18181 ggctcattca ttccaggttt tgaagatcaa ttagttggtc ataaatcagg tgaaagtcta
    18241 gatgtaaatg taactttccc agaagattat caagcagaag atcttgctgg aaaagaagca
    18301 gtcttcaaag tgacaatcca tgaagtaaaa gcaaaagaag tgcctgaatt agatgacgaa
    18361 ttcgcaaaag atgttgatga cgaagtatca acattagacg aattgaaaga aaaatatcgc
    18421 aaagaattag tagaaacaaa agaaaaagca gcggacgatg caaaagatga agcagcgatc
    18481 cgtatggctg ttgaaaacgc agaaatcgtt gaattgccac acgtgatggt tcatgatgaa
    18541 gtacatcgtt caatggacga attcttgaac aacatgcaac gtcaaggtat ctctcctgaa
    18601 atgtactatc aattaactgg ttctacagaa gaagacttgc ataaacaatt cgaaggtgaa
    18661 gcagaaatgc gcacaagaac aaatcttgtg atcgaagcaa ttgcagctgc tgaaaatctt
    18721 caagcaacag aagaagaaat caatgcagaa atccaagaat tatcagaaac atacaacatg
    18781 ccaatcgaac aaatcaaacg cgtgttgaca gaagatatgc tggaacatga tgtgacaatg
    18841 aaaaaagcaa tcgaattgat cacaggtaca gcgacagaag cgaaagaata gaaatcaagt
    18901 aataaatcat ttctgaagaa gctacttcta gaaaattgtt tattcgaaat aagggactgt
    18961 gacacgattt tgtcacagtt ccttattttt tgttaagagt aaaaaatgca aaggtatagg
    19021 tcagttgatc ctttgttaaa cactttttct ttttactaaa ctatgttatg atagtaaaag
    19081 actgtaaagt ctttgatagt ttcttgcata ctttttgcaa gttaaggggt gaagaccatg
    19141 tatgacaatc caagtagcaa tgagaccgta cgctgttctt tttgcgggaa aacccaagaa
    19201 gaagtgaaaa agatcgtagc tggtccaggg gtctacatct gtaatgaatg tatcgaccta
    19261 tgtaaagaaa tcattgatga agaattttat gaagaagctg tacgcgaatt cactgaagtc
    19321 cctaaaccac tagaaattct tgaagtattg aataactacg tgattggaca agatcgcgca
    19381 aaacgctcgt tagctgttgc cgtatacaat cactataaac gtgtcaatca gcaagcacaa
    19441 gaaaacacag atgatgttga actgcaaaaa agcaatatct gcttgatcgg acctacaggg
    19501 tctggtaaaa catttttagc acaaacatta gcaaaaacat tgaatgtacc ttttgcaatc
    19561 gcagatgcga caagtttgac agaagctggt tatgtggggg aagatgtaga aaacattttg
    19621 cttaaattgc ttcaatctgc tgattacaat gtggaacttg cagaaaaagg gatcatctat
    19681 attgatgaga tcgataaaat tgcgcgtaaa agcgaaaatg tatctattac tcgtgacgtt
    19741 tccggcgaag gtgtccaaca agccttgctg aaaattctag aaggaactgt tgcaagtgtg
    19801 ccacctcagg gcggacgtaa acatccacat caagaattta ttcagattga tacgacgaac
    19861 atattgttca tcgtaggtgg tgcatttgat ggcatcgaaa cgattgtgaa gaatcgtatg
    19921 ggcgaaaaga cgattggatt tggtacacaa aaccaaaaac tttctgaaga cgaaagtgtc
    19981 atgcagcata tcattcctga agatttacta aaattcggat tgatccctga gtttatcgga
    20041 cgattacctg ttatggcagc tttagagaaa ttgacaacag atgatctggt acggatcttg
    20101 actgagccga aaaatgcgct agtcaaacaa tatcaaaaac ttctttctct agataatacc
    20161 gaacttgaat tcgaacctga agcgttgaga gccattgcga aaaaagcaat cgaacgcaat
    20221 accggtgctc gaggacttcg ttccatcatt gaagaaatca tgatggatgt gatgtttgat
    20281 atcccttcaa atgaaaacat cgaaaaagtg attatcacaa aagaagcagc tgaattgtct
    20341 ggtgagccaa cagtaatcta taaagattca gagaaaaaag cgggttgatg aaaagaggct
    20401 gccgtaaaag tcattttggc ttttgcgaca gccgcttgct ttattcatca ccttgttaag
    20461 gagagagacc atgaaagtcc atcaagctga aattatcata agtgcagtgg caccaaaaca
    20521 atatccagat acacaattgc cagaaatagc tctggcagga cgatcaaatg tagggaaatc
    20581 ttcttttatc aacacattga ttgatcgaaa gaatttagcc cgtacttccg gtaagccagg
    20641 aaagacccag acattgaatt tttatttgat cgagaacgca ttacattttg tcgatgttcc
    20701 gggatatggg tatgcaaaag tatccaaaac tgagcgagca aaatggggaa agatgatcga
    20761 gacatattta acgactagag accagctacg tgcggtcgtt tctttagtag atttgcgtca
    20821 taagccttct caagaagata tccagatgta tcaatttttg aagtattatg atatccctgt
    20881 aattgttgtt gccactaagg cagataagat ccctcgtggt aaatggaaca agcatgagag
    20941 cgaaatcaaa aaagcgttgg attttgatcg caatgacacg tttattctct tttcttccgt
    21001 tacaaaacag ggaaaagaag aagcgtgggc agcaatcgaa caagcaattg aatcataaaa
    21061 agaataaaaa agacttacca gtcaaactct ttcataaaag tttggctggt aagt
//
LOCUS       DAFBQH010000042        20942 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.043, whole genome shotgun sequence.
ACCESSION   DAFBQH010000042 DAFBQH010000000
VERSION     DAFBQH010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.043"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            168..362
                     /locus_tag="LK694_001681"
     CDS             168..362
                     /locus_tag="LK694_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052548.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="LK694_001682"
     CDS             631..930
                     /locus_tag="LK694_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052549.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="LK694_001683"
     CDS             complement(1027..1461)
                     /locus_tag="LK694_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5052550.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="LK694_001684"
     CDS             complement(1693..2403)
                     /locus_tag="LK694_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBK5052551.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="LK694_001685"
     CDS             complement(2393..3253)
                     /locus_tag="LK694_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBK5052552.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="LK694_001686"
     CDS             complement(3258..3896)
                     /locus_tag="LK694_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBK5052553.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="LK694_001687"
     CDS             complement(3911..4210)
                     /locus_tag="LK694_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5052554.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="LK694_001688"
     CDS             complement(4253..5737)
                     /locus_tag="LK694_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBK5052555.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="LK694_001689"
     CDS             complement(5757..6218)
                     /locus_tag="LK694_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5052556.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="LK694_001690"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="LK694_001690"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBK5052557.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="LK694_001691"
     CDS             7521..8291
                     /locus_tag="LK694_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK5052558.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="LK694_001692"
     CDS             complement(8564..9391)
                     /locus_tag="LK694_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5052559.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="LK694_001693"
     CDS             9959..11194
                     /locus_tag="LK694_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBK5052560.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="LK694_001694"
     CDS             complement(12193..12453)
                     /locus_tag="LK694_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBK5052561.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="LK694_001695"
     CDS             complement(12480..13781)
                     /locus_tag="LK694_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5052562.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="LK694_001696"
     CDS             complement(14073..14531)
                     /locus_tag="LK694_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK5052563.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15875)
                     /locus_tag="LK694_001697"
     CDS             complement(14544..15875)
                     /locus_tag="LK694_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HBK5052564.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17328)
                     /locus_tag="LK694_001698"
     CDS             complement(15886..17328)
                     /locus_tag="LK694_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK5052565.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="LK694_001699"
     CDS             complement(17315..18079)
                     /locus_tag="LK694_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBK5052566.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="LK694_001700"
     CDS             complement(18183..19058)
                     /locus_tag="LK694_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK5052567.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19313..19397)
                     /locus_tag="LK694_001701"
     tRNA            complement(19313..19397)
                     /locus_tag="LK694_001701"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19361..19363),aa:Leu,seq:caa)
     gene            complement(19415..19486)
                     /locus_tag="LK694_001702"
     tRNA            complement(19415..19486)
                     /locus_tag="LK694_001702"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19452..19454),aa:Gln,seq:ttg)
     gene            complement(19496..19568)
                     /locus_tag="LK694_001703"
     tRNA            complement(19496..19568)
                     /locus_tag="LK694_001703"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19533..19535),aa:His,seq:gtg)
     gene            complement(19630..19714)
                     /locus_tag="LK694_001704"
     tRNA            complement(19630..19714)
                     /locus_tag="LK694_001704"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19678..19680),aa:Leu,seq:caa)
     gene            complement(19735..19805)
                     /locus_tag="LK694_001705"
     tRNA            complement(19735..19805)
                     /locus_tag="LK694_001705"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19771..19773),aa:Cys,seq:gca)
     gene            complement(19851..19922)
                     /locus_tag="LK694_001706"
     tRNA            complement(19851..19922)
                     /locus_tag="LK694_001706"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19888..19890),aa:Gln,seq:ttg)
     gene            complement(19934..20006)
                     /locus_tag="LK694_001707"
     tRNA            complement(19934..20006)
                     /locus_tag="LK694_001707"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19971..19973),aa:His,seq:gtg)
     gene            complement(20009..20082)
                     /locus_tag="LK694_001708"
     tRNA            complement(20009..20082)
                     /locus_tag="LK694_001708"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20048..20050),aa:Trp,seq:cca)
     gene            complement(20089..20169)
                     /locus_tag="LK694_001709"
     tRNA            complement(20089..20169)
                     /locus_tag="LK694_001709"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20133..20135),aa:Tyr,seq:gta)
     gene            complement(20171..20246)
                     /locus_tag="LK694_001710"
     tRNA            complement(20171..20246)
                     /locus_tag="LK694_001710"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20211..20213),aa:Phe,seq:gaa)
     gene            complement(20282..20354)
                     /locus_tag="LK694_001711"
     tRNA            complement(20282..20354)
                     /locus_tag="LK694_001711"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20319..20321),aa:Asp,seq:gtc)
     gene            complement(20364..20436)
                     /locus_tag="LK694_001712"
     tRNA            complement(20364..20436)
                     /locus_tag="LK694_001712"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20401..20403),aa:Val,seq:tac)
     gene            complement(20452..20523)
                     /locus_tag="LK694_001713"
     tRNA            complement(20452..20523)
                     /locus_tag="LK694_001713"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20488..20490),aa:Glu,seq:ttc)
     gene            complement(20538..20627)
                     /locus_tag="LK694_001714"
     tRNA            complement(20538..20627)
                     /locus_tag="LK694_001714"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20591..20593),aa:Ser,seq:gga)
     gene            complement(20667..20740)
                     /locus_tag="LK694_001715"
     tRNA            complement(20667..20740)
                     /locus_tag="LK694_001715"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20704..20706),aa:Asn,seq:gtt)
     gene            complement(20750..20865)
                     /gene="rrf"
                     /locus_tag="LK694_001716"
     rRNA            complement(20750..20865)
                     /gene="rrf"
                     /locus_tag="LK694_001716"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6603 a         4390 c         3331 g         6618 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa actgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
    19261 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat tataccggcg
    19321 gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc
    19381 aattccgcca cgccggcaaa tttaaaaata ataactgggg tagctggatt cgaaccaacg
    19441 catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa taaagggcga
    19501 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
    19561 acattcgcca tcataaaaga tgttatttaa tttctcaata aaaaactaag atgaagacat
    19621 cttagtcaat accggcggcc ggggtcgaac cggcacgccc tcaacgggca ctggattttg
    19681 agtccagcgc gtctgccaat tccgccacgc cggcaagttg atttattagt actaaggcgg
    19741 taaccggatt tgaaccggtg atgaaggttt tgcagacctc tgccttacca cttggctata
    19801 ccgccattat actattatca atctataaaa taaagaataa attatagaaa ctggggtagc
    19861 tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc
    19921 caagaataaa aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg
    19981 tgcgttaacc acttcgccac attcgccatg gcaggggcag caggaattga acccacacta
    20041 acggttttgg agaccgctgt tctaccttta aactatgccc ctataaaatg gaggagagtg
    20101 gattcgaacc actgaaccct aaggaacgga tttacagtcc gtcgcgttta gccacttcgc
    20161 tactcctcca tggtggcgcg ggacagaatc gaactgccga cacacggagc ttcaatccgt
    20221 tgctctacca actgagctac cgcgccatat tttaaaatta ttaattgaat aacaagaacg
    20281 acggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca tgttaacccc
    20341 tacaccacgg aaccagttca ttatggaggt taacgggatc gaaccgctga ccctctgctt
    20401 gtaaggcaga tgctctccca gctgagctaa acctccaaca ttcaattaaa atgacccgta
    20461 cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg
    20521 gccaggtaaa tttgctacgg agagtaaggg attcgaaccc ttgagacagt ttggcaccat
    20581 ctacatgatt tccaatcatg ctccttcggc ctctcggaca actctccaga gtgaaagaag
    20641 ctcaatactc tttctcaaaa tgagaactcc ggcagtagga ctcgaaccta cgacatcatg
    20701 attaacagtc atgcgctact accaactgag ctatgccgga ataacatcag cgtggcgacg
    20761 tcctactctc acaaggggca acccctcact acaatcggcg ctaagaagct taacttctgt
    20821 gttcggcatg gttacaggtg tatccttctc gctatcgcca ccacactgtg gtgttatctt
    20881 ttattgagta aattttgttc actcaaaact ggatttgaag tcacataagt ttttttccga
    20941 gt
//
LOCUS       DAFBQH010000043        20627 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.045, whole genome shotgun sequence.
ACCESSION   DAFBQH010000043 DAFBQH010000000
VERSION     DAFBQH010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.045"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(2..1990)
                     /gene="treP"
                     /locus_tag="LK694_001717"
     CDS             complement(2..1990)
                     /gene="treP"
                     /locus_tag="LK694_001717"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBK5052568.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLVYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            2213..2926
                     /gene="treR"
                     /locus_tag="LK694_001718"
     CDS             2213..2926
                     /gene="treR"
                     /locus_tag="LK694_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBK5052569.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            3003..3449
                     /gene="msrB"
                     /locus_tag="LK694_001719"
     CDS             3003..3449
                     /gene="msrB"
                     /locus_tag="LK694_001719"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBK5052570.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            3619..4221
                     /gene="ruvA"
                     /locus_tag="LK694_001720"
     CDS             3619..4221
                     /gene="ruvA"
                     /locus_tag="LK694_001720"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBK5052571.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            4234..5235
                     /gene="ruvB"
                     /locus_tag="LK694_001721"
     CDS             4234..5235
                     /gene="ruvB"
                     /locus_tag="LK694_001721"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBK5052572.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            5264..5842
                     /locus_tag="LK694_001722"
     CDS             5264..5842
                     /locus_tag="LK694_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK5052573.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKVLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            5894..6376
                     /locus_tag="LK694_001723"
     CDS             5894..6376
                     /locus_tag="LK694_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBK5052574.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            6493..6867
                     /locus_tag="LK694_001724"
     CDS             6493..6867
                     /locus_tag="LK694_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052575.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(7667..9019)
                     /gene="brnQ"
                     /locus_tag="LK694_001725"
     CDS             complement(7667..9019)
                     /gene="brnQ"
                     /locus_tag="LK694_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBK5052576.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            9166..9756
                     /locus_tag="LK694_001726"
     CDS             9166..9756
                     /locus_tag="LK694_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBK5052577.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(9884..11233)
                     /locus_tag="LK694_001727"
     CDS             complement(9884..11233)
                     /locus_tag="LK694_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5052578.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(11401..12141)
                     /locus_tag="LK694_001728"
     CDS             complement(11401..12141)
                     /locus_tag="LK694_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK5052579.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(12154..13746)
                     /locus_tag="LK694_001729"
     CDS             complement(12154..13746)
                     /locus_tag="LK694_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBK5052580.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(14314..14610)
                     /locus_tag="LK694_001730"
     CDS             complement(14314..14610)
                     /locus_tag="LK694_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052581.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            14786..15511
                     /locus_tag="LK694_001731"
     CDS             14786..15511
                     /locus_tag="LK694_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBK5052582.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            15504..16346
                     /locus_tag="LK694_001732"
     CDS             15504..16346
                     /locus_tag="LK694_001732"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBK5052583.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKAVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            16349..16870
                     /locus_tag="LK694_001733"
     CDS             16349..16870
                     /locus_tag="LK694_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBK5052584.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(17123..17686)
                     /locus_tag="LK694_001734"
     CDS             complement(17123..17686)
                     /locus_tag="LK694_001734"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBK5052585.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            17937..18206
                     /gene="rpsO"
                     /locus_tag="LK694_001735"
     CDS             17937..18206
                     /gene="rpsO"
                     /locus_tag="LK694_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBK5052586.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            18394..20520
                     /gene="pnp"
                     /locus_tag="LK694_001736"
     CDS             18394..20520
                     /gene="pnp"
                     /locus_tag="LK694_001736"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBK5052587.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
BASE COUNT         6739 a         3963 c         4166 g         5759 t
ORIGIN      
        1 actaagattt tacttcagct ttaccgactg cttcgcccgg gcgaacaagt cctgattggt
       61 caagagaaag tcctgcaact ttttcattat tggtcaaagc aacaataatg tcagttttct
      121 tgccagcttg ttctatttct ggcaaatcca tttcagcaat cagggtctct ggtgtgacaa
      181 ggtatccttc tttcacgaat acattgaaag gaacaccctt tagttcaaca gtatctaatc
      241 ccatgtgaac aagtacttcc agcccctcat tagaaagaat accaatggca tgttttgttt
      301 caaagacact cgtcacttta ccgtttactg gtgcatagac tttgccgttt gacggttcga
      361 cagcataacc gtcacccatc attttttgtg agaaaactgg gtcatttact tcagttattt
      421 ccttgatggt ccctgcagcg actgcgaata atgtttcttt tgttgcaact gccgtaccag
      481 ttgctgaaac aggttcgaaa gactgtggcg ttgctgttgc gccatttgct gtttgaagtt
      541 gtacatcagc agcattttca ggaacagcag gatcaatctt attcaaaatt ccctgacgtc
      601 taaagatgat cgtcaagcca aatggaatga tgacagcaat gatcattgcg atgatgaaag
      661 gtacccatgt ttctgcttgg atagctagta ttcctggcaa accgcctact ccgatcgcgt
      721 tagctcgaac acccatcaag ttagcaaaca tgccagctaa tccagagccg accattgcag
      781 cgacgaaagg atagacgtat ttcaaattga ttccgaacat agcaggttca gttacgccta
      841 agtaacaaga aatcattgct ggaatcgaga tttgttcttc ttttttattc ccacgatgta
      901 agaagatgat ggctaagact gcagagcctt gggcgatatt tgataaagca atcatcggcc
      961 ataaattcgt tgatccgaaa tctgcaatca gctgcatatc gatggcattg gtcatatggt
     1021 ggagaccagt aatcactaaa ggagcataca ggaatccgaa tacggcacta aacaaccagc
     1081 tgatagaaga cgtcaatcca gtatttacga tatttgagat ccagcttccg actgtccagc
     1141 cgataggacc taaaataacg tgtgcagcta aaacagttgg taacaatgaa aacaatggaa
     1201 caaaaatcat cgagatcgac tgagggatat atttgcggaa gaagatttct agatacacta
     1261 acataaaccc tgccagcatg gctggaataa cttgcgcttg ataaccaatc atctggactt
     1321 gagcaaaacc aaaatcccaa aatggaatat ctgctgcggc tgtggaagct acactataag
     1381 catttaataa ttgcggagaa actaacgtaa ttcctaaaac gattcctaat atttgtgtag
     1441 tacccatctt ttttgcaata ctccaagtga tacctactgg aagaaaatgg aaaattgctt
     1501 cacccggtaa ccacaaaaag gcattgacac cattccaaaa cttcgagtgt tcaacgattg
     1561 ttccaccaag actttcaaac tggatacctt caagtacatt tctgaatcca aggatcaatc
     1621 caccgacaat gattgcagga atcagtggtg taaagatttc tgctaaaacg gcaatagcac
     1681 gttgaacggg atgaaggttt tgttttgcag ctgcttttcc ttgttctttg gaaacccctt
     1741 caactccaga tactgcaacg aagtcgttat aaaaagtaga aacatcgttt cctatgatga
     1801 cttggaactg tcctgcattc gtgaacatcc cttttacgct aggtatatct tcgatcgctt
     1861 tttcgtccgc ttttcctgga tcattcagga caaaacgcat acgagtcgca caatgactaa
     1921 ctgcagcaat attttctttt ccgccgatat catgtaacag cttctcggca tctgcttgat
     1981 attttgccat atcaataaac ccctttctct taacttgtct atgcaagttg tttcttcatt
     2041 tactatactt tttttcggag agagttgcaa gcgttttatt aaaaagtacg agagaaaatg
     2101 atatcgtttt aaaaagctta aaataagctt ttcttaaaaa taggaacatt gaaatacaaa
     2161 aagagctgta gtatagtgaa gaaatgatgg aagaacgagt gagggaaaaa acatgaataa
     2221 gttcaatgaa atttttttag atttggaaca gaaaatttta gcgaaagaat atcctccgca
     2281 ttcgctttta cccagtgaga accaattgat caaaatttat ggtgtttcac gagaaacaat
     2341 tagaaaagcg ctgaatcttt tgactacgga aggttatatt caaaaaaaac aaggaaaagg
     2401 ttcgatcgta ctggatcgga atcgcttcga ctttcctatt tctggattga ctagctataa
     2461 agagctacag gagacccaac gtattccaag tgaaacgatc atccatacgt tagaagaaac
     2521 agaagtgaca aaagcattga acgagatcac tggctgggag atcggtgcgc ctgtttggca
     2581 tttgatacgc gaaagaaaaa tcaatggcga agtagttata ttagacaccg attatctatt
     2641 aaaggaaatc gttcctattt taactccagc acaagcacaa ggatcgatct atgaatattt
     2701 tgaaaatgag ctttctttga cgattgctta cgcgcaaaag gagatcacag tagaagaagt
     2761 aacggaaatc atgaaaacag cgatggatct tcacgatgac acttatgcag tagtagtcag
     2821 aagcctcgtt catctggaag atacccgctg tttcgagtat tctgagtcgg ttcaccgatt
     2881 agataaattc cgtttcgttg agtttgcccg acgccgaaaa gtgtaaatag ttcaaaagaa
     2941 agcaaattgt tggcatctgt ccactttggc gctatgatag gatcacaaag ggggcatttt
     3001 taatggataa accaataaga ccagaaaaag aagaactgaa agaaaagtta tcacccatgg
     3061 cttatgcagt cacacaagaa aatgcaacag aacgaccatt tagcgggaaa tatgatgatt
     3121 tttacgaaaa ggggatctat gtagatatcg tcagcggtga accgttattc agctctgcag
     3181 aaaaatatga tgcaggttgt ggctggccat cattcagcaa accaataacg aaaagacaag
     3241 tgcgcgaaaa agccgatttt tctcatggca tgcatcgaat agaagttcga agcaaagaag
     3301 cagattctca tctcggacat gtgtttaccg atggaccagt agatcaaggt ggtctacgtt
     3361 actgcatcaa ttctgcctca ctgaaattca ttccatatga aaagatggat gaattaggtt
     3421 atggggaatt caaatcactt gtagaataat ggaaatacac aaagagcggt acaatacggg
     3481 gatttactcg atcgtccgct ctttttttat tcaagcgatt gctatttgaa agaaacgctc
     3541 cccagactcc ttttttcaca aggtgaaatg tgctacaata aagggaacgt atgtgtgcaa
     3601 aagaaagagg actagaatat gtatgaatat ctgacaggtg tggttacctt tatcaacccc
     3661 tattacatag tagtagaaac caatggtatc ggctaccaga tcgcactagg aaatccttac
     3721 cgctacagta gcaaactaaa tcaaaaaata aaaatttatg tccatcaggt aatacgtgaa
     3781 gatgcgcatc ttctgtatgg atttgattcg ctagaagaaa agcaactgtt tttgcgatta
     3841 gtaagtgttt caggaatcgg tccgaaaagt gcccttgcaa tcatggcaag cgaagaccac
     3901 tccggtttga ttcaagcagt agaatccagt gatgtcactt atttgacgaa atttccagga
     3961 gtagggaaaa agacagcaca acaaatgatc ttggatctaa aaggaaaatt cggtgagctg
     4021 agtatcgata caccttttag tctatttgat gaagcaaaca caaaagacgc tacagctcta
     4081 tctgaagcaa tggaagctct ctcggctctt ggttacagtg atcgagaaat caaacgtgta
     4141 gaaaaacaat taaaagaagt agataatcag acaacggatg agtatctacg ccaagcactc
     4201 aagctaatga tgaaaaaata aggaggagac caaatgacag atgaatactt gttatcacca
     4261 gaatctggcg aaaatgaact gagtttggaa aaatctcttc gaccacagct gctttctcaa
     4321 tatatcggac agagaaaagt caaacaagaa cttcagattt acatcgaagc tgccagaaat
     4381 cgggaagaac ctttagatca tgttctgttg tacgggcctc ctggattagg aaaaacgacg
     4441 atggccatgg tcattgctaa tgagatgacc gtaaatattc gtacgacgag tggccctgct
     4501 atcgaacgtc cgggtgactt agtagcaatc ttgaatgaac tggaagcagg ggatgtcctg
     4561 tttatcgatg agatccatcg attacctaga gtggtagaag agatgcttta ttcagctatg
     4621 gaagatttct atgtagatat catggtagga caagggccaa ccgcacatcc ggttcatttc
     4681 cctcttcctc cctttacttt gattggcgcg acgacacggg caggtatgct ttctgcacct
     4741 ttacgagatc gatttggcat catttctcat atggaatatt atgacgaaca ggatctgcag
     4801 gagatcgttt tacgttcttc ggatattttt caaacagaga tcattgaaga aggggcattg
     4861 gaaattgctc gccgatcacg aggaacgcct agaatcgcta atcgcttgct caaacgggtt
     4921 cgtgattttg cgcaagtcca aggagacggg aaaatcgatc gtcctatagc ggatcaggca
     4981 cttactttgc tacaggtcga tcaggctgga ttggattatg ttgaccagaa attgttgcga
     5041 acaatgatcg agttatatgg cggcggacca gtagggttaa gtacgctttc agtaaacata
     5101 ggagaagagc gtgagacggt agaagatatg tatgaaccgt atctgatcca aaaaggattt
     5161 ttaaagcgta cacctagagg gcgaattgct actgcttatg cctatgaaca ttttggttac
     5221 gactatcata tatgattttt gcttttagat tggaggaaaa tgaatgagtg atacaacacg
     5281 tacgaaacaa ttgatcaaaa acgtattgat tgatttgtgc caaacgcaat cttttcgaaa
     5341 aatcagcgtc caaaaaatcg caaatgaagc aaaaatcaat cgacaaacat tttattatca
     5401 ttttacagat aaatacgatt tgcttcgttg ggtctattgt gaggatgcat tgcattattt
     5461 agatccacaa tgtctttcac ttgataattg ggaagagcaa gcattgaaag tgctgaaagc
     5521 aattgctgag aagaaagaat tttatgcaaa tactgtgctt tctgacccag aaattttaca
     5581 aaaagagttt tctcaactga tccaaaaatc atttatcaag atttttgaac aaatggatga
     5641 agaaagacaa ctttctgcaa gagacaaact attttatgct cgtttttttt cttacggttg
     5701 cagtggtgta ttagtcaatt ggatcacgaa tgaatatgaa gaaacaccga tggaaatcgc
     5761 tatgcagctt ttccgtttag ccaaggatac ggaattatat tcttatcgtc tttatgcaaa
     5821 agaagagttg gaagaacagt aatagaaaaa ttttttgttg gaaaagcaat aatcgctaaa
     5881 aggagatggg gaaatgacaa aaattttatt tgtttgtttg ggaaatattt gccgttcacc
     5941 gatggcagaa ggcttgctga gaaaacgaat agcaatagaa ggaagggaag cagaattttt
     6001 tgtggattct gctgctacaa gcacgtatga agttggaaag acaccgcacc ctggaacgaa
     6061 aaaaatatta aaccaagaaa aagtagatat gacgaatatg gttgctcggc aaatcacacc
     6121 ccacgatttt gaaacttttg attggatcat tggcatggat caagaaaatg tagaagaact
     6181 gaaacgaaga gcaccacgct cagcgcaggg gaaaatccat ttgtttttaa gtagcattcc
     6241 tggaaaagaa aaagaaaatg ttcccgaccc atactatacc aataactttg atgagacata
     6301 tcacttgatc acagaaggcc tgaatcattg gtggaatatt tggattgacg aaaataaaat
     6361 aacaaacaat ttgtaaagtt gacgctttct ttttaaggcg atgttagagc atttccttgt
     6421 ttttttgtaa aatgttaggt aaaataaaat taattaagta acactttctt agctaaagga
     6481 ggagtgcatt cgatgaacga agagacagta tcgccaatga agaagcttac gaagttagtt
     6541 actcatactt cgtatcaact ttttgattct tttgtaaatg acactgaaga acatgctaag
     6601 aatgtacgat tgcaaggaaa gtttattctt gagctgcaaa aagctgctac tgaacatagt
     6661 ctagttatct tacaagtaag agcgaatcgt ttgagtagca agtatgaaac gatctcaggc
     6721 tgggtggcga caaaaacggt cgaaaaagac cgagtgatga tccgcctaca aaacgaagag
     6781 cagcagatcc ggatggttcc tgtagaacaa attgaaaaaa taacaatcct ttcaccagca
     6841 ggggaacagg aacgattatc aaaataattt ataccagata gaaaagcacg tatgagcttg
     6901 taccatcaat cattactaat caaaaataac cgaatgcttt aagctgatat cagtaaaacg
     6961 gaacgtttta ggatgagtat cacagcatga cagcattggg ttgattagtt gaatcgattg
     7021 gatggtacaa gtttttttga taaaaaaagg tcctgctagc cggggagcta gcaggataag
     7081 gagttaaaaa tgaaaaagtg ttgttagggt tgtttgttgg tatgcttata taatagtcac
     7141 tagttgtgaa tattttgtga cgaaagtttt cgaaagttgt taagtttttt tgcgaaaact
     7201 atttttatat catatatatg tctaaaatct atattataaa agaaatgttg tttgatcagc
     7261 ttctcttcgg tattagttca aaacaaaacg gcaaagtata tggagctact ttgccgtttt
     7321 gtttctctaa tactcaggtc agtttataga cgctcaataa acggtgttca aaagccagtt
     7381 tctcggtatt aagtcaaatt tatgcaaaaa tgtgagaagc cattttcaca aaatatttct
     7441 taatacttga aactaagcgg ctttttcacg acctctcaat aaacggtgtt caaaagtcag
     7501 tttctcggta ttaagtcaaa tttatgcaaa aatgtgagaa gccattttca aaaaatattt
     7561 cttaatactc gaaactgagc ggctttttca cgacctctca ataaacagtg ttcaagagac
     7621 agcctatcgg caataaggct gaacgtctct ttcacaacct cttttatcaa tctgctactt
     7681 cttctttctt agcgaaactc caaatcagtc cgataacaaa accgataact gctggtacga
     7741 tccaccccat ccccaatttg aaaaacggca agtagctttc cgcaacatat aaaagatttt
     7801 gaacaatagg tgtttgtttg atagtttctg ggcaagcgtt caacccatca aaaatcgaag
     7861 caatcaatgt aaaataagta gtcattcgat aaacagccgt acgctgctta aacaaaggac
     7921 caactaacac tagtaaaatc agtgtcattg ctaatggata gatgaacatc aaaacaggca
     7981 acgaaaattg aatgatattc gtcaagccta cgtttgccgc taaacaagga aggatactag
     8041 caagtgcaac aaagaatgcg taatttcttt gcggaaacaa atctgtgaat gtttctgcaa
     8101 aagctgtaat caaaccaata ccggtcttca agcaagctaa tataacgata aatgctaata
     8161 agatactgcc ataagtacct aaataatgat cagcaatctg agccagcgtg atcccgccat
     8221 tttcacttag agcaaagcta cctaagctca tagcacctaa ataagcgagc aaagtataaa
     8281 tgattcccat caatataatg ctgatcgcac cagatttgat cgtgtcttta gcgatttcag
     8341 caggttttgt tacacccatg ttttggattg ttgtgacaat gatgatccca aatgctaaag
     8401 cagcaagcgc atctaatgtg ttatatcctt gagtaaaccc tgtgaaaaaa gcatgctctt
     8461 gataatttgg ctgaataggc gcttgattta cagaacctag tggattcaaa aaagcaagga
     8521 gaagcaaagc acctaataaa accaaaaatg caggattcag gaactttcca acataatcta
     8581 atatctttgt cggttttctt gaaaaccacc aagccagcac aaaaaataca atactgaaaa
     8641 ttgctagata tagcgtctgt ctgctaggat cgataaatgg tgctaaacca atctcgaaag
     8701 atgtggttgc aaggcgaggt aaagcaaaaa acggtccgat aactaagtaa agcaagatgg
     8761 taaaaaccat cgcatatgtt tgatttatcc gttttgctaa ttcatataca cctgagcttt
     8821 tcgatacacc aatggcaatc acccctaaga agggaagacc gataccggta accaagaaac
     8881 ctagattcgc ccaaaaggta gcagcgcctg cttcttgacc caaatgaacg ggaaagataa
     8941 gatttccagc accaaaaaat agaccgaaaa gcatggagcc tataaaaaga tagtctttaa
     9001 atgataattt ttttgtcata cataactcct ttacggtttt tttaaaagta acaaaatttc
     9061 gaaaaatatg caacaatttt cagaaaattt cctatataaa acgacggtta tttaataacg
     9121 taagtgataa aataaaagcg ctgaatcatc tgaggaggaa tcatcgtgac aaagtcgaga
     9181 ttagaagcat ttactgatgg agtggtagcc attgttttga gagttcttgt attagagatc
     9241 aagatcccta atacatctag ctttcattct ttatgggtca tccgaaatac attattagct
     9301 tataccatca gctttatatt tgttgcggtg atctgggtga accatcatcg tatatttcag
     9361 atggcagaac gaattaacta ccgagtggtc tggtcgaata tctttttgct cttttggctg
     9421 actttatgtc cttctgttac tagttgggtc gggcgtaacc ctgaagcttt ttggcctgag
     9481 atggcttatg tcttagtata cacgatgtgg agtttttctt ttggtatctt gatgaggcaa
     9541 atcatgaaag caaataaacc agattcccat gtcgtgagag ttttgtcaaa ggaccgtcga
     9601 agtcttatct ccatgatgat taacttatgc ttgattgccg gtgtcttttt tgtaccgttg
     9661 atcgggttat tcggacgttt ttttgtatct ggtatttgga ttttttccta taaaaaagca
     9721 gatgcctatt atcagcggtt gtttccgggg aaatagaaaa gaggctggga cagaagcttt
     9781 taactccaag aaataagccg aaatttcaca aaaatttaag aagcaatttt cgtaaattcc
     9841 ttcttatttc tcgaagctga ccacttctgt cacaactact ttcttacttc ttacgttcat
     9901 atttcatatg atagattagt aatagaagca agaaccacac aggtgttagt aataaagcga
     9961 ttctcgtatc ttttcctagg aacaagacga cacacacaaa ggccatgtaa agaaatacca
    10021 gataattact aaatgggtac aacggcattt taaatgaatg agcttttttg ccgaggatac
    10081 ttttacgata tttcatgtgc gtgtagacaa gcatactcca gataaacaag aaacaagtag
    10141 tggcaatact gcttactagt gtgaatactt cactaggcat cacgtaattc aagataacag
    10201 aaatgaaaat gacgatagta gaaaatgtca aagcattccc tggcacatga tgtgttgtta
    10261 actttttgaa ttttttaggg gcactgcctt cttgcgctaa agaacgaagc atacgtcctg
    10321 tactatatat tgcactgttg caggcagaag ctgctgcact taatacgact aaattgatca
    10381 aggaagcagc aatcgtgatc ccaatttccg aaaatacttc tacaaatgga ctgttatctg
    10441 gattcaagct gttccatgga tagattgcca taatgacaaa aagagatcct aagtaaaata
    10501 agatgatcct gataggaata ttattgatag cttccggcaa cactttttca ggattttctg
    10561 tttctccagc gactaatcca actaattcta tcccagcaaa agcaaaggtt accatttgga
    10621 aagctaagat aaatcctttt gcaccagttg gaaagaatcc gccatgagac catagattcg
    10681 tgatactggc tgtgccaata tctgtcgtat agtgggtgag gatcatataa gctcctacga
    10741 ggataaaggc aatgatcgct acaactttga tcaatgcaaa ccaaaactct aactcaccaa
    10801 acaatgagac agccaccata ttcaagcaaa gaagaagacc taatgcaatc agttcaggaa
    10861 gccactgagg aacaccaggc gcccagtagc gtacatacat tccgatggct gttaaatcgg
    10921 ccattgcaat cgatacccag cagaaccaat acgtccagcc agtgatgaaa gccgcttttt
    10981 tgccaagata ttctgcaaca aaatccagaa aagaatggtt attggtgttt gataacagaa
    11041 gttcgcctaa cgcacgcata atgaaaaaac agatgcatcc agtaataaga taggcgagca
    11101 atatcgaagg gccagctagc tggatagact ttccggaacc taaaaacaac cccgttccaa
    11161 tcgctccgcc aatagaaatc agttggacat gtctattttt cagtccacgc tgcagttctt
    11221 gctcttcatt catttttaaa tcatcctaac taaaaaagtt taattacttc catattaaaa
    11281 gagttttctg aaaaacacaa ctaagattcc aaaattttca gaaatttacc ttttatgagt
    11341 aattcataaa aaagaactgc ggtaaacacc gcagctcttt gtcccaatca gctattgatc
    11401 ctaagcattt aaaatacgtg aaaggaattc ttttgtacgt tcttctttcg gatggacaaa
    11461 gatatcttct ggtgtacctt cttctgcaat cacaccttta tccataaaaa tgacacggtc
    11521 agatacatct tttgcaaatt ccatctcatg cgtcacgata agcattgtca agccagtatg
    11581 tgctaagtct ttgatcgtat taagcacttc gccaaccatt tcagggtcta acgccgaagt
    11641 aggttcgtcg aatagcaata catctggatt cattgataat gcccgagcaa ttgcgacacg
    11701 ctgtttctgt ccaccagata attgtgctgg tttggcttcg atataacgat ccataccaac
    11761 tttttccaga ttttcgattg ctactttttt ggcagtttct ttatctcgtt tcaggacagt
    11821 tgtttgacca gacatacagt tttccaagac attcatattg ttgaacaggt tgaaggattg
    11881 gaaaaccata cctagattcg ttcgatattt aggtaaatta tatttaggag ctaatacatt
    11941 ttctccatta taaatgatct cgccgcctgt tggtttttcc agtaaattga tacagcgcaa
    12001 gaaagtagat ttaccagaac cggaagaacc gatgatcgtc acaacttcac ctttattgac
    12061 tgttacgctg atatctttca atacttcatt ttcaccgaag ctttttctta gatgtttgat
    12121 ttcaataatt ttttcattca tgattcttcc tccttattcc tgcaaatttg aattgtcgat
    12181 ttcttcaagc ttggtataag ctgaaggacc atcgattttc ttttctaccg cccgcaggat
    12241 tctggtgatc gtaaaggtca tgactaagta gatgatcccg acgatcgtaa atgtttggaa
    12301 gaattggaaa ttcgcaccgg aagcagagtt tccttggaag aacaaatcag ctacgccgat
    12361 tacacttaaa actgctgtat ctttgatatt gataacaaac tcatttcctg ttgctggcaa
    12421 gatattccgt aaaacttgtg gaataacaac ttttctcatc gtttggccat gggtcatacc
    12481 aatggcttgc gcagcttcga actgcccttc atctactgca aaaatccccc cacgaacgat
    12541 ctctgtcatg taagctccag tattgagaga gacaataaac aaagcggcaa tcgttcggtt
    12601 caatgaaatg ccaaaagcga gtgccagacc gtagaagatg accattgctt gtaccatcat
    12661 cggtgttccg cggaatactt cgacatagat agaaagaagt gcatttccga ttttttggaa
    12721 gaagcgagtc agtgcatttc ctgattcagg taccgaacgg aagacaccaa tcagtaatcc
    12781 aagtgtagta ccgacaactg tcccgatcaa tgcgatgaac aaggtaagtc cagcacctcg
    12841 taagaacata ttgccgtatt gtttccaaat cgtagtgaaa tcagaaatga tgccaggttt
    12901 ttcttcacta tcagttgcag caggctgatc tttgactgct tggtccataa ttttggttcg
    12961 ttcatcgtta gagatgccag aaagaatctg gtttactttt tggatatccg ggtcgccttt
    13021 acgcatacca acagaaatct gaacatcttc cgggtttgtc tggaagccat ctgcaccgct
    13081 gaattcaagc atttccaagt ctttgttcac actagttgcc gttacacctt ccggacgttc
    13141 gctgacataa ccatcgatca ttccagaagc taaagctgtt ctcattgctg agaaattatc
    13201 cattgcttgt tgtttctgca catctggtat ctgatcgatc acagtatagt ggaatgtatt
    13261 taactgagca gtaatctttg ctccagaaag atctgctaaa ctttttgctt ttgcatattt
    13321 gctatctttt tgaactacta ctactaattg tgattcgtaa taaggatcgg taaaatcaat
    13381 ttcttttcgt cgttcagcag tagggctcat tccggcgatg atcgcgtcga tcttgcctga
    13441 ttgaagggca ggagccagac catcccattt cgtttggacg atgactaatt ttttccctaa
    13501 accatctgct acttttttag cgatttgaac atcataaccg ccggcgtaac tattttggcc
    13561 ttgaattggc aaggcattat tctcatctgt ttgttgagac cagttgaagg gtgcataccc
    13621 agcctccata cctacgcgaa actcatcgct cggtgttttt tcatctgcat ttgccagaga
    13681 tcccacactg aatagtgaga agacaaaagc aaatagtacg gcaactgaaa ccatattttt
    13741 tttcattttt ttctcccttt ctgattatat gatacacgag cggtctagat gaaatgcggt
    13801 aaagctttat ctgaaaaaat aaaaagcgac cgatttatag atataaaaag gccgctaaaa
    13861 ttccataagg tgataaacct ctcacaaaac atagcgcaac ttagcatcac tgctaagaca
    13921 gtccgcaagc tcttaacttg cgtcccaaca tacactccaa gcaaaaaagt gcacatttcg
    13981 gcgatcctcc ctattcctgc ttctccatgt ttgcttcact ccacagggtt aatgattctc
    14041 gcgacctcta cctcattgat tgaggcatct cgtattcagt tatttataca aaaaaagttt
    14101 acgatactcg agaggctttg tcaatctata actgctgttt tttgaaaaaa tactgataaa
    14161 tatagaaatc tttactttta ccgaaaaaac aatcaaaatg tgaatcatct attcattgca
    14221 aacgatagta taattataca caaaaatgta tgtaatacaa taaaaaaaga actagaacag
    14281 aaaatttcct gtgtccagtt ccatctcgat catttaagct gccgctttct ttggctcacg
    14341 tcctaggatc gcttgcaaaa gaacaacgac gccataaatg gctaatatta gataaatcaa
    14401 acttgagtat tgggtgattc cccagcccca tgttcgatat acggctaatg taatggctaa
    14461 aacaattgcg gcaatcacgt tgaataaagc aaaagcccaa agatttccat tttctttgcg
    14521 tacaagataa aaaacagaga aataaacgct taacgctaac caggcgatac aagccagaat
    14581 aacgccccaa taaataaaga atggtaacat atcgattcac ctcactttta tacagcagta
    14641 ttgtaacacg ttttcattct tttgtcagtg aacattgtga taaattgttc aaatgcccat
    14701 aacacttttt tattcccatt tttctgactt ttcaacaata aatttgttcg agtatgggct
    14761 ataatggaaa gtgaggtgac aaaacatgaa acaattaaca gtagacgaag cagcaaaaga
    14821 gatattagct tatcagcaaa ttacattttt gaccggtgca ggggtatcca caccttctgg
    14881 agtgccagac tatcgatctt tgtcaggaat ctaccacggg atggagcagc cggaatactt
    14941 gctgagtcat caagcaatgg tgaatgaacc ggataaattc tatcaatttg tcaaaaagat
    15001 ttatcatcct gaagcaaaac ccaatatcat tcatttggaa atggcacatc tagcgaaaga
    15061 aaaaaatgtt tgggttgttt cccaaaacat cgacggtttg cacgcaaagg ctaaaagccc
    15121 gcaactcgtc aattttcatg gaagtttgta tcaatgtcat tgtcgaaaat gtcaacaaac
    15181 ggtagactgg aaagaatatc ttcacagcga caaacatcaa gcatgcggag gacaaatacg
    15241 cccagacatc gtgttatatg gtgaagggtt tcaagatgga gtcatggagc aggcggctta
    15301 tgccgtcagc caagcagagc tgatcgtcat tgttggaacg agttttcaag ttcatccgtt
    15361 ttgtgatctg gtacagtttc gccagccgtc agccaaaatc ttagtaatca atcaaacgcc
    15421 tgtttattta gcggagcctt atacgtttat tcaagaagac ggagcggttg ttttcaaaaa
    15481 aatacaagaa ttaggagcag attatgactg aattcaaaac cgaaaaagaa aaaatgattg
    15541 caggcgagct ttattttgcc aatgatcctg agctggttgc cgaccgtatg tttgcaagaa
    15601 gtcaaagcca aatcatcaac caagcagaat cagcggaact gcgatcccaa ttgttgaaag
    15661 agacttttgg ccgtacaggg aaaaaaatct atatggagcc agttatcaat tttgattacg
    15721 gatataatat atttgttggg gagaattttt atgcaaattt caattgtaca tttttagatg
    15781 tcagcacgat tgaaatcggt gataactgca tgttcgctcc gaatgtccag ctttacacgg
    15841 cgacgcatcc actacatcca gtgaaacgta acagcggtct ggagtacgca aaacccatta
    15901 aaatcggtaa taatgtttgg ttaggcggag gagttatcgt cacgccgggt gtcactctag
    15961 gggacaacgt agtagtcggc gcaggttcgg ttgtgaccaa atcttttccc gacaatgtag
    16021 tgattgcagg aaatcctgct cgtatcatca aggcagtaga ggaagaacta acagaagagt
    16081 ctcttgaaac attacgtcat aagatcgata tgatcgatcg agagttggtt gctttattag
    16141 aaaaaagaat ggatacggta actaaaatag gacaagtgaa aaaagaagaa aaccaagcag
    16201 tttatgatgc aaaaagagag cagcaagtat tggataaagt tgttagtctg ctgaaaaata
    16261 agggatataa ggaaacaata acagatacgt atgttgattt gatgaaacac tcgcgcgaat
    16321 accaaaacaa aatgaaggag gaataagtgt gatcgaagga gagacacggc gaaaacaaat
    16381 catgcaaacc ttggcaaata cagaaaaacc agtgagtgct agcaagtttg cgaaaacttt
    16441 tggagtcagc cgtcagatca ttgtaggcga tattgcttta ctacgagctg caggtaaaag
    16501 tatcgtggca acagcaagag gttatatcct cgaaacagaa gaaaccaata ctgggcacat
    16561 cagtaaaatc gctgtccagc atggaaaaga tcaaacagaa gaagaactga gactaatcgt
    16621 tgaaaatggt ggtgaaatca ttgatgtaat tgtggaacat cccctttacg gtgagttagc
    16681 aggaactttg cacatcaaaa cgccacaaga tatcgattcc tttatgagac gttatcaaaa
    16741 aagtaatgct actttgttat ccgaattaac aggcggcatc catctgcata cgatccgcta
    16801 cccagaaaag catatgctga aaaacatcaa acaaaaacta gctgaagcag gaatcctcta
    16861 cgaaggataa catcgtagct gctacttttt atcctagaaa ctattctatc atctctgaga
    16921 taaacaccca tatggaagaa ataagaaaaa ataagaaaag atgcgtatcc aatacacaga
    16981 attttttgga attgcgattt ctagatgtct atctaaattt cgattagtgg agaaacataa
    17041 aaaagggaag tgcagctttt gagcactccc cttttttata aagctttctt gtagcatagt
    17101 atcgaaggaa cctgccaatt ctctactcga tcaccaatac gccttctttt agtgcaaatg
    17161 gattttgatc gttgatgtgg tcatagaaca tcacaccgtt gatatggtcg atttcatgtt
    17221 gaacgacgat cgattcataa ttcttcaaac gaatcttgtg tttttcacca ttcatatcat
    17281 aataagacac agtgattttg gcatggcgaa caacatatcc tggtacttct cgatcaacag
    17341 ataaacagcc ttcaccttcc cctaaacaag catcttgaac agaatggctc aaaattttag
    17401 gattatacat aacagtactt agagaaggtc catctgcctc tgggtcagga cttggtacgt
    17461 gaacagcaat gattctttta gaaatatcta attgaggagc agctaagcca acaccgcctc
    17521 gtaagtgtag ttcttcagct ttgattggat cttggctgtt ttttaagaac tcaagcatct
    17581 cttttcctaa agagatatct tcttcgctta atggaagaga aacttctttt gcaacttcgc
    17641 gtaaggtcgg atttccttca cgaatgatat catccattgt aatcatggaa tatgtacccc
    17701 tttcttaaaa gactgtgttt caagtctatt ttcgcattat agtttaccac aaaaatggaa
    17761 aagtagtagt aagattgttt gaaaactacc ttttcttaat gattaactac ttgattttca
    17821 acccggtttc aggtaaagta tagctgtgtg ctctaggcac atgacctgcc acttggttaa
    17881 gcgaatcacc aacgttttac tcagtcgcag gggaataatc gaaagaggtg aaagaaatgg
    17941 caatttcaaa agaacgcaag aatgaaatca tcaacgaata cgctcgccat gaaggggata
    18001 ctggttctcc agaagtacaa atcgctgtat taactgaaga aatcaaccac ttgaatgaac
    18061 atgcacgcgt gcacaaaaaa gaccatcatt cataccgcgg cctaatgaaa aaagttggtc
    18121 accgccgtaa cttgttagct tacttacgta aaactgacgt tcaacgttac cgcgaattga
    18181 tcaaacgttt aggcttgcgt cgttaatttt agtcaaaaag agtgggattc acatgaatct
    18241 cacttttttt actcgtatat caattaagta aaattccgtg atcaagctag atggactcct
    18301 actaaccaaa tgaaagctgc tcaaaagacg gctttcggca gttttcattt gtttagtagc
    18361 gtcttgcttt cacgagaagg aaaaggagaa gttatgtcag aaaaacaagt attcaaaacg
    18421 acatggggcg gccgtccctt acaaatcgaa gtcggtcagc tagcaaaaca agcaaacgga
    18481 gccgttttag tccgttatgg agatacagta gtcttaagtg cagctgtggc ctctaaagaa
    18541 gcaaaagata cagacttttt cccattaacg atcaattatg aagaaaaaat gtatgcagca
    18601 gggaaaatcc ctggaggttt catcaaacga gaaggccgtc caagtacgga agcaacattg
    18661 actgcccgtt tgatcgaccg tccaattcgc ccgatgtttg cagaaggctt ccgtaatgaa
    18721 gtccaagtaa cgaacatcgt gatgagtgta gaaacagact gctcgccagc tatggcagca
    18781 atgctaggtt cttctttagc actatcaatc tcagatatcc catttgacgg cccaatcgca
    18841 ggcgtagaag ttggtcgtgt aaatggcgag tacgtcttga atccaacagt agaacaagca
    18901 gaacaaacag atatcgaatt gactgttgcc ggaacgaaac aagccatcaa tatggttgaa
    18961 agcggcgcga aagaagtttc agaagaagac atgcttggtg cattgctgtt tggttttgac
    19021 gctatcaaag agttagttgc tttccaagaa gaaatcgttc aagcagtagg gaaagaaaaa
    19081 atggaagtga ctttactgca agttgatgaa gtattgaaaa aagaaatctt tgatgcttct
    19141 tacgctacaa tgaaagcagc agtaatgact gaagaaaaac ttgctcgcga agataatatc
    19201 gagcaggtga agatcgatat ccgcgaagct tatgctgaga aatttgcagg tcatgaagat
    19261 gaagatcact tgttgaaaga agttaaacaa atcacagaag atcttgaaaa agatgtcgta
    19321 cgtgaattga tcaccatcga taagatccgt cctgacgggc gtaaattgga tgaaatccgt
    19381 ccattgtcat cagaggtcag cttattgcca cgtgtacatg gatcaggttt gttcacacga
    19441 ggacaaacac aagctttatc agcttgtaca ctcgcacctc taggagagca ccaaatcatt
    19501 gatggtttag gggtggaagt cagcaaacga ttcatccacc actacaattt ccctcaattt
    19561 tctgttggtt ctacaggacg tgcaggctct ccagggcgtc gggaaatcgg acatggcgca
    19621 ttaggtgaac gtgcgttagc acaagtaatc ccttcagaag aagaatttcc ttatacgatc
    19681 cgtttagtag cagaagtatt ggaatcaaat ggttcttctt cccaagcaag tatctgtgct
    19741 ggcacattag cattgatgga tgcaggtgta ccaatcaaag cgccagtcgc cggtattgcg
    19801 atgggacttg tttctgatgg tgaaaactat acgatcctaa ctgatatcca agggctggaa
    19861 gatcatcttg gcgatatgga ctttaaagtt gccggtacaa aagatggtat cactgcttta
    19921 cagatggata tcaagattca aggaatcaca gaacaaatcc taactgaagc attaactcaa
    19981 gcaaaacaag cacgtatgga aatcttagaa gaattgactt caacgattgc tgctccacgt
    20041 gaagaattaa gccaatatgc accaaaaatc gaaatgatcc agatcgaacc tgctaaaatc
    20101 aaagatgtta tcggtaaagg cggcgatacg atcaacggaa tcatcgatga aactggtgtg
    20161 aaaatcgata tcgatcaaga tggaaaagta agtattgctt cagctgatgc agaaatgatc
    20221 aaaaaagcta tcaagatcat cgaagatttg accaaagaag tcaaagttgg tgaagtttat
    20281 ctagggaaag ttgtccgtat cgaaaaattc ggtgcattcg tcaacttgat caaaggaaaa
    20341 gatggacttg tccatatttc ccaattagca aacgatcgtg tcaataaagt cgaagatgtt
    20401 gttaaactag gtgatgagat ccttgtgaaa gtaactgaaa tcgataaaca aggacgcgta
    20461 aatctgtcaa gaaaagcaat gttgaacgaa gatggttcaa cgaaaaatga agaaaaataa
    20521 tttaattcat aggaataatg tacttgtgat cgtgaaggtg acagggggac aataattatt
    20581 cgaaaaaagg ggactgtgac aaaagccgtg tcacagtcct ttttttt
//
LOCUS       DAFBQH010000044        20568 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.046, whole genome shotgun sequence.
ACCESSION   DAFBQH010000044 DAFBQH010000000
VERSION     DAFBQH010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.046"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(24..722)
                     /locus_tag="LK694_001737"
     CDS             complement(24..722)
                     /locus_tag="LK694_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBK5052588.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="LK694_001738"
     CDS             complement(715..2067)
                     /locus_tag="LK694_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBK5052589.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="LK694_001739"
     CDS             complement(2290..3690)
                     /locus_tag="LK694_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBK5052590.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="LK694_001740"
     CDS             3851..4588
                     /locus_tag="LK694_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBK5052591.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="LK694_001741"
     CDS             4713..5192
                     /locus_tag="LK694_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBK5052592.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="LK694_001742"
     CDS             5189..5956
                     /locus_tag="LK694_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052593.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="LK694_001743"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="LK694_001743"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBK5052594.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="LK694_001744"
     CDS             complement(6859..7461)
                     /locus_tag="LK694_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK5052595.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="LK694_001745"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="LK694_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="LK694_001746"
     CDS             8142..8726
                     /locus_tag="LK694_001746"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBK5052596.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="LK694_001747"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="LK694_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBK5052597.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="LK694_001748"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="LK694_001748"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBK5052598.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="LK694_001749"
     CDS             10627..10806
                     /locus_tag="LK694_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052599.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="LK694_001750"
     CDS             10828..11853
                     /locus_tag="LK694_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBK5052600.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="LK694_001751"
     CDS             12028..13272
                     /locus_tag="LK694_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBK5052601.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="LK694_001752"
     CDS             13425..14372
                     /locus_tag="LK694_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK5052602.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="LK694_001753"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="LK694_001753"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBK5052603.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="LK694_001754"
     CDS             complement(15274..15516)
                     /locus_tag="LK694_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052604.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="LK694_001755"
     tRNA            15637..15709
                     /locus_tag="LK694_001755"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
     gene            complement(15828..16466)
                     /locus_tag="LK694_001756"
     CDS             complement(15828..16466)
                     /locus_tag="LK694_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBK5052605.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16589..17221)
                     /locus_tag="LK694_001757"
     CDS             complement(16589..17221)
                     /locus_tag="LK694_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBK5052606.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17572..17871
                     /locus_tag="LK694_001758"
     CDS             17572..17871
                     /locus_tag="LK694_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBK5052607.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            18366..20531
                     /gene="acm"
                     /locus_tag="LK694_001759"
     CDS             18366..20531
                     /gene="acm"
                     /locus_tag="LK694_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBK5052608.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
BASE COUNT         7187 a         3520 c         4038 g         5823 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt ttaatccatt gatacatctg tctttttgtt taaaggaaat
    15781 aaagtatgct ttcttagcaa tagagcatgt attatttcta taaaaattta ggagaataaa
    15841 acgactagca atatagctgt gaaatcaaat atcacatgaa ctgcccaact tgtaatgatc
    15901 gagttgcttt taatggcagc ttggttgaat aacaatcttg ccaccccttg tatcaaaagg
    15961 atgtgtgcta cagtgtgtaa aatatttcca ttatcataag tactgtaatg gataaatcca
    16021 aagatgattg ctgacaagaa actagcgaca ggaatgctga acttactaga aaaaatagcg
    16081 agaaaataca ctgagaacaa ttcttctcca agtatcatga taggcaaaat aataaataat
    16141 aacgggctga aagcctcatt tattggattg cctgttaaac cccatttcaa aatttgttgc
    16201 aaaaataccg agactgcggc actgattact acattgataa aaaaatattt ggctatattt
    16261 ttgacaggag cgacaggttt cgagaacatt tttttcgtat cttttttacc aaaaacaagt
    16321 gtaaatgcta taaatccagt gatagttaaa gaaaaaatgc taataatgga tggcgcaaat
    16381 agcaacccta acaaaccgag tacaacgaca atagtgccca atatcccttt tttaagttct
    16441 attttttttc ctaaaatgtt attcatgata ataactccta ctattcagtt tttgaagata
    16501 gagtcagtat acatgaagag ttaaaatagg caatgttttt ttatggatat ttgtattgct
    16561 aaaaatttat cttatatatt cattttgatt atactacttt tatttgtaaa aattttctag
    16621 caaaaccaag tgcttgcaac tgattaagcg tacaattttt tagatgttcc gagcgtactt
    16681 caatcatttc aaaggagttg ctacttaaat cgatattttt taaatcagta tcaaaaaagc
    16741 tagcattttc taaatcatta gtagaaaaca ttattttagt gagggaacag tctacaaaat
    16801 ctgcgcttga taagacagag tgctcaaagg tactttcttt tatgtttgaa ccagataaat
    16861 ttacatagtc cattttacaa ttctcgaata acgtgttccc aatataactt tccatcaatt
    16921 tagtacctgt taatttacaa ttgacaaatt tcacgcgatg caacatgctt tttgtcaata
    16981 tacagcctga aaaatcacag ttttcgaata tacagtcggt aatctccatt ctttgaaaat
    17041 cattggaagc aaaaaatgca tacttgaaaa tagatttgta aataattgga tggcagatta
    17101 tttggttttc gatgacctct ttttgtgaaa ggatattata cagttcaaaa tcttcgtttt
    17161 catagatatg aaaaaaggat cctttattca agcttgcttc attaacttta ggaaaaatca
    17221 tatttaatta cctccatttt cataaagtca tattaacaaa agctaaacca acttacatta
    17281 gcagaaaatg agataggttt tcgccttttt cgataacgat agtgtcgcta tctcttgaaa
    17341 cgttcttaga attgccctga gagacacaaa ttaggcgtaa ttctaataaa aatcttagaa
    17401 taaaaatttt tactgattta atttagaatg tttttaaaat aaaaatcgtt gccataacag
    17461 tgtattgaaa ggttttaaca atccagtaat gatttaaaaa aatctagatg tagcgtatac
    17521 tttgtttata gaatgtagtg ggaagaaata tatttttggg aggaaatcgt gatgtctaaa
    17581 agtgaagaaa tagaaaacat ggtactaaaa ttatatgatc tcgttttaaa tccagagatc
    17641 aaagaaaagg aacgtatact tttgatagac gcaaagacag gcttagaaaa aggtcagtat
    17701 tatcctaaag tcataaataa tttggaacgt agtttacgtc cgttagcaat caggggagaa
    17761 ttgtctaaac cagtatcacc gttttatatg gagatatcaa caataggaaa atttgaaaaa
    17821 gaattaggtc gaggaatggc ttcggcaccg attacttttg ggcatttata aaaacgagtt
    17881 attcatctca atgaattaaa aagtatagtc aatctcataa tgcaggctag gttcatttat
    17941 ttgaacagag tctgtatttt tttattctaa ataaagaaac agatgtttct ttatattgta
    18001 ttttatcctt taattaacac aaaaacagct agtataaact aataatgtta attaaaagtg
    18061 tattttatga aactatcgtt atttattaat gttgcatttt tgtaagttaa aaattggaaa
    18121 tatagattga aaaaatgaaa gcaataagta aaataaaatg aatatatgcg aaaaaataat
    18181 ttacattagg aaaaaccttg taaaaacagt tttttggcat atttattttt tataaatatt
    18241 tgtaaaataa ctatcaattt tttagttaaa agattggaaa tgagtggggg aggaattgaa
    18301 gaaaggattt ttgagagatg atatagtagg ttttattttt agttaaaata ggggagatag
    18361 aaaaaatgaa aaagtgtaga aatattattt tatcaatgtt atttattatt actaatataa
    18421 catcactgat tcctgttcat gtctatgcgg atgcaggcag agatatcagc agtaatgtca
    18481 cttcgttgac cgtggaccca acgaatatta cagatggagg aaacataaaa gtaaaattct
    18541 cgtttgacga aaaaaaacaa aatatccaac caggtgatta tctttggatc aattggccaa
    18601 gtgaaggaaa tattcgcggt gaaggattcc aaaaagaaat cccattaatg attgaaaata
    18661 aaaatgttgg aactttaacg gtcagaaaag attctgcgca ggttgtgttt aatgaaaata
    18721 ttaaaaacct agactcagta gaaggttggg ggcaatttga gatacaggcc agaaacgtaa
    18781 ccgataccgg tgaagaaaat acgggaccat tcaccgtgac gagcggtgat aaaacagcta
    18841 ctgtaaatgt gacaaagcca gcttctggtt cttcttctag cgttttttat tataaaacag
    18901 gagacatgct acctgaagat actaagcaca ttcgatggtt tttgaatatt aacaataacg
    18961 gcacttatgt tgaacaacca gttaaaatca gtgatgaaat ccaaagtgga caaagattag
    19021 atccaagtac gtttgaaatt aatcaaatac acttaggtga acagaaggtt tatagaggag
    19081 aagagggaat ccaacaattt ttacaagatt tccccagcgc tacatttaat ttttcagtaa
    19141 ctgataatta cattgaaata actataccaa aaaactttgt caatcttagg aaaataatgg
    19201 tgagttataa gacgatcata gaaaacccag aacaaataaa ttttgagaat cattcggaag
    19261 cttggtttaa agaatttaat aaacctgctg ttgatggaga aagtttcaat catacagtta
    19321 aaaatatttc agcttctggt ggagtcaacg gtacagttag aggcgaattg aagattttca
    19381 aatatatcaa tgacacagag attggtatcc caaatgtgac tttcgaatta agacgagcag
    19441 atgaacagcc tattcaagga cagtctagta tcctgttgac ttcgaatgaa cagggagaag
    19501 ctagtatcaa aggcctgcaa gtaggagact atgttgtaaa agaaaaagaa gcacccaatt
    19561 ggattgattt tgatccacta agttcaaatg aactcaaatt ctccataaac gaaaatgata
    19621 cagagggagt cagtttacct atttataaca agaaaaaggt tacgaacatc acagcaacta
    19681 aaatttggaa cggtggaacc actccaagac cgagtattta ttttaaatta ttcagagcaa
    19741 gtacaaacaa ttgggaacca gttcctgatg cagaaacaaa acgattagac aatggaataa
    19801 cttcagtgac ttgggaagat atccaacagt atgatgatag tggaaacgaa tatacgttta
    19861 aagtgcaaga agtagatcaa aacggaaatg attatgtccc ttcaggttat caaaaaattg
    19921 aaaacgggct agtcgttaca aatgagaata aagaagtcat ttcagtctca ggacaaaaaa
    19981 catgggaaga caatgagaac caagacggca aacgaccgac gacgatcaca gtaaatctat
    20041 tagccgacgg tcaaccgatc caacacaaag aagtaagtga aaaagatgat tggcgctatc
    20101 gtttcacaaa tctgccaaaa tacagagacg ggcaagaaat catctatact gtaacggaag
    20161 acaacgtgcc cgaatacagt acgacgattg acgggtataa catcaaaaac agttatacac
    20221 cggaaaaaac aaatatatct ataccaggta gccatataac accgtggaaa aattttccag
    20281 agaagacaga gagagagaaa attagaaatc tattctctca aaaattgcaa atatttgctc
    20341 atacaggaga atccagcgaa caaagaagca attatattag taagagagct gtacctaaac
    20401 agataacaaa taaatcaaaa agcattcttc ctaaaacaag tagtaaaaaa tctagttttg
    20461 ctgtaatcat tggtttattg atagtaacta tctgtgctgg gatattctta caaaaatata
    20521 aaaaagttta gttagaatat aatatttttt gttaaatatg actgaaat
//
LOCUS       DAFBQH010000045        20523 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.047, whole genome shotgun sequence.
ACCESSION   DAFBQH010000045 DAFBQH010000000
VERSION     DAFBQH010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.047"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..111
                     /locus_tag="LK694_001760"
     CDS             <1..111
                     /locus_tag="LK694_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBK5052609.1"
                     /translation="ISREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            420..572
                     /locus_tag="LK694_001761"
     CDS             420..572
                     /locus_tag="LK694_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052610.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(569..844)
                     /locus_tag="LK694_001762"
     CDS             complement(569..844)
                     /locus_tag="LK694_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052611.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            1298..1504
                     /locus_tag="LK694_001763"
     CDS             1298..1504
                     /locus_tag="LK694_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052612.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            1700..1867
                     /locus_tag="LK694_001764"
     CDS             1700..1867
                     /locus_tag="LK694_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBK5052613.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            1893..2237
                     /locus_tag="LK694_001765"
     CDS             1893..2237
                     /locus_tag="LK694_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK5052614.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            2242..2523
                     /locus_tag="LK694_001766"
     CDS             2242..2523
                     /locus_tag="LK694_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052615.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            2626..2940
                     /locus_tag="LK694_001767"
     CDS             2626..2940
                     /locus_tag="LK694_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBK5052616.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            2918..4612
                     /locus_tag="LK694_001768"
     CDS             2918..4612
                     /locus_tag="LK694_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBK5052617.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            4632..5810
                     /locus_tag="LK694_001769"
     CDS             4632..5810
                     /locus_tag="LK694_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK5052618.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            5773..6459
                     /locus_tag="LK694_001770"
     CDS             5773..6459
                     /locus_tag="LK694_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBK5052619.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            6459..7619
                     /locus_tag="LK694_001771"
     CDS             6459..7619
                     /locus_tag="LK694_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBK5052620.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            7629..8504
                     /locus_tag="LK694_001772"
     CDS             7629..8504
                     /locus_tag="LK694_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052621.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            8501..8812
                     /locus_tag="LK694_001773"
     CDS             8501..8812
                     /locus_tag="LK694_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052622.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            8802..9155
                     /locus_tag="LK694_001774"
     CDS             8802..9155
                     /locus_tag="LK694_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBK5052623.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            9145..9546
                     /locus_tag="LK694_001775"
     CDS             9145..9546
                     /locus_tag="LK694_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052624.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            9539..9943
                     /locus_tag="LK694_001776"
     CDS             9539..9943
                     /locus_tag="LK694_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052625.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            9955..10563
                     /locus_tag="LK694_001777"
     CDS             9955..10563
                     /locus_tag="LK694_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5052626.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            10583..10945
                     /locus_tag="LK694_001778"
     CDS             10583..10945
                     /locus_tag="LK694_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052627.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            11147..14578
                     /locus_tag="LK694_001779"
     CDS             11147..14578
                     /locus_tag="LK694_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBK5052628.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVKKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            14629..15366
                     /locus_tag="LK694_001780"
     CDS             14629..15366
                     /locus_tag="LK694_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5052629.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            15376..17667
                     /locus_tag="LK694_001781"
     CDS             15376..17667
                     /locus_tag="LK694_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5052630.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            17691..19817
                     /locus_tag="LK694_001782"
     CDS             17691..19817
                     /locus_tag="LK694_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052631.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            19834..19983
                     /locus_tag="LK694_001783"
     CDS             19834..19983
                     /locus_tag="LK694_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052632.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            19980..20426
                     /locus_tag="LK694_001784"
     CDS             19980..20426
                     /locus_tag="LK694_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052633.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            20428..>20523
                     /locus_tag="LK694_001785"
     CDS             20428..>20523
                     /locus_tag="LK694_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBK5052634.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEE"
BASE COUNT         7229 a         3081 c         4291 g         5922 t
ORIGIN      
        1 attagccgtg aagtgggtta ttcagaaaga agtatacaac ggatgaagtc ggaagctcta
       61 atagagtttg cagaagcata taaacacgga aaaataattg cttataaata atttggcggt
      121 tttttggcgg aatgatggcg gtttttagac atttaccagt gttattatgg tagtgtcgaa
      181 agattaggaa acaggatcga caaaataaac gaagggaggc aatctccctc atcgttttaa
      241 acttcttctt agatagacag caactaatta tgaagaaaag gatgtgaatt caactcctcc
      301 taaattcttc atcggttgct gtttattttg attagacagc agcactcaca aaactaaaca
      361 tgttcttgtc gtctttcaac tgctgctgtc tattaattta tgtattggag ggaagaaaaa
      421 tgaaattatc agtagaaggc actccagaag aaatacaaga attgctccaa gctattggta
      481 gtagcaagga gcaaaaggta agtgtttcta attcagatat tgacggtatt tgcaattgtt
      541 taaacagaga aaaaatatct aatccttact aagagaaaat atgttcgtta atgaatttcc
      601 aattttcctc tgtagctcta ccttgataat ttttttgcag ttcacagatg aataaggaat
      661 cgttattatc aagtactgta tttaattttt cacacatttg atttggattc aaattagttt
      721 taactaacca aaaagatttt tgtagcttta tatatgcccc aaattctttt attatttcaa
      781 atactttatc atacttttgc cctgggttat taagatcata actaatcatg tagggtttat
      841 acatgttgtc ttgcacatcc ttgttttata tttcagcgga ccactcgctg ataaataaaa
      901 ttatacgctt agtatttatt ttcacaatat taatttatcg taataacttt tgagtaaata
      961 gaacaaaaaa cctgcactag tttccgctag tacaggcagt gactatatcg gttgataatc
     1021 tagcatatat taaaaataat tgcaagaaaa gaaatttata gtggtttata ccaaattatc
     1081 aaagcaatgt tattttgttg ctgtctattg ttttttaatt attcacacga taactaaagg
     1141 tgggtgaaga gaaatgattc cattaataat ttcaattttt gcgctctgtc ttaatgtcta
     1201 tatgattgga tttaaaaatg ggcaaaataa aaaatagtag cagccaagaa taattttata
     1261 gtgtcactgt ggcggaaagg gtagacgcta agcatgtgtg ctaggtcaat gcttcggcaa
     1321 ccatgcaagg ttcgattcct tgccagcgac attaaatgcc tatgacggtt acgactaccg
     1381 aaaaaagatc gttaagaagc tatacggtgc tacgtacggc aatgtagtaa gtgtgctatc
     1441 tgtacaccac caagcttcgg tcactgtggc ggaagtagaa gacgcagcgg taaatggcga
     1501 gtagcctcgt gagagcctgg taagttctcg tgagtggtgc aatcccactc cagcgacttt
     1561 aagcaaccga ggcatcggcg gtttaaaaat ataggggtgc gcaatttcgt acgcgttttg
     1621 tgcatcgtgc aagttactat tacatattag atcactcttt gagtggtctt tttatttttg
     1681 cacaaaggag gaaacaacaa tgtatagacc acaatactta gaacagaagt atgaagtaat
     1741 cactgttcat aaaggtaatg gcgaaaaagt atatgagtat agaagaccaa taaagagcga
     1801 tacatataaa cgaaaggaaa acaatgaagt tattccattg tatggcaaaa gaatagctaa
     1861 gcattaaata agattgcgaa aggagacgga acatgaccga ggaattctat agatggctat
     1921 tacagttgac aagagaagat cgtttggtta agttctatca gtctcctaaa tggcgcaggc
     1981 ttagagagaa agcgatgaaa cgagatcact atgaatgcca agagtgtaga agactaggta
     2041 agtatcatag agtagagaac gttcatcata taaaggaagt caaggataga cctgacttag
     2101 ctttagattt agataatctt atttgtttat gtgttgaaca tcataatgaa gttcatggca
     2161 gatatcttac agcattagat aaacaagaga agaagataga aagctttgct aacttcgatg
     2221 caagtgaaag gtggtaagtg catgatcatc aatgataatg gcagagagta tgatacagaa
     2281 aagattgaag agtattcatc ttatactcag ggattaatta aacgtttgat atacgttcgc
     2341 tatgtaggta ttagggatct gttatcagat aactgttgta gtaaatacaa agtgaatcaa
     2401 gtaagagaag cgttgaataa agataataac gtcgaaagaa taaaaaatgt ttttggatat
     2461 agtattgaag agattaatta ttacattgac ttcgctgaag ctttcattcc gatggtgaga
     2521 taaccccccc ttaaaataaa tcgcaaattt tttgggggtg atgaaacgga gggggctgtc
     2581 aggaaaagag attttttcga actttatcat gaaaggaggg ctaaaatgtt taaaaacgaa
     2641 ttgtctcaaa atcgctacag agaaaaatta cgccgctctt taataagcca attggaaagt
     2701 cagaaaacaa atattgagcc attcttagat aatgttgatc gttatatcag tttatgggaa
     2761 acggcgatat cactggaaga agatatatcc gagaacggca ttagactgga gaatggtaaa
     2821 aagaatgaat cagtagcgtt gcttgtttct gtcaacaaac aaatgggatt gatgttggat
     2881 aaacttgcca ttactcctga attggtaggt gaagcaaatg aatcaattcc tgagttataa
     2941 gcatattgaa aattggttca aagctataga agaaggcact atcaaggtat gcaaagagca
     3001 attattgcta aaaaattatc tagaagaaag agtctttact agagaagata tttacttcga
     3061 taagcagatg gtagaggatt caatcaatat accagcacaa tactttccat tcgaattaat
     3121 tccgtgggaa aaatttctac aatgttttat ttatggtgtt cgatggaaaa aagataaaac
     3181 actagtgttc aatagatatc tttcattaat gggacgtggt aatggtaaaa ctggttttgc
     3241 ttcttggaac aacttctttc tactaaccgc taaacacggt attaaaaatt atgatattga
     3301 tatctatgcc aataatgaaa gccaagcaaa gactagtttt gatgatgtat ttaaagtaat
     3361 taaagatcat cctgatttag ataaaaaagt atttaaagct acgaaggaag ttattcaaaa
     3421 tatcgctaca aacagcaaac ttcgttataa cacggcaaat gctagaacaa aagatgggaa
     3481 gcgaccaggt gcaaaccgct ttgatgaaat tcacgaaaat gaagattatt caatgataaa
     3541 tgtggctact tctggtggtg gtaaaattcg agattataga gaattttatg atacaactaa
     3601 tggtcatgtt cgtggtggtc cgcttgatga cattatagaa gaatcaaaaa tgattctttc
     3661 tggagaactt ggaattgaca aggatggagc agaattttct agtttgtttc catttatttg
     3721 tcgcttggat aacgataatg aagttgatga tcccgacatg tgggaaaaag cttgtccaac
     3781 tattaattac aatgcagatt taaaacggaa aatgtttcaa gaatactctc aaatgcaacg
     3841 taatgctggt ttaagactta cgttcatgac caaacgaatg aacagaccta tggaagatac
     3901 acgatttgct gttgcttcat atgatgatgt tctgcatacg aaagaaaaag aatttcctga
     3961 aaaaatggat gaagtgatag gaacagtcga ttttgctgat agacgagatt ttgccagcgt
     4021 tgggttgcta ggaaaatacg ataaagatgt ttattttaca caacatactt ttatccacga
     4081 atcagccctt cgattacaaa acatcaaacg agaggttata gatatttcta tagatcaagg
     4141 aaaatcacag atcgttcatg gaaaaaatat agaagctgat tatattgtag gttggtttct
     4201 tgaaatgagt aataaatatt atattaaaaa aatcgctatg gatatgtacc gtgcaaaaat
     4261 attgaagccc gctttagaag aagcaggttt tactgtggaa attgttcgaa gcggatctgt
     4321 tacacatggt atgttaaaag atctggttga tgaccttttt attaatcaac gtttattttt
     4381 tggtgacgat gcgattatgc gttggtattg catgaatgta tatgaagagc atatttctaa
     4441 tggaaatata cgctatgaaa aaatagaacc tgaaactaga aaaacggatg gctttttttc
     4501 attccttcat ggtttgaatt ttttagatga tatttatgat tctgctcctg taacagtcac
     4561 aaatagctca gtagaaaata caggaactgg atttactcct ctagtattct aacttgaaag
     4621 gaggtgagaa agtggggatt tttcaaaagg cggtaggata cttcacaaaa aaagcaacgg
     4681 ttcctttaga agaatacttt tgtaaattgc aagttgattt tgtgtatcga aaatttgcga
     4741 ttgaaacttg tattgatttg attgcaaatg cgatgagtaa agcggaattc aagtcatatg
     4801 aagatggaaa aaataaaaag aatgatcttt actataggct gaatgtagct cctaataaga
     4861 aaaataatgc aacagaattt agaaaaaaac tgatcaggag attaatattc tacaatgaag
     4921 tattgatcgt ttctccgtct aataattcta gcgaaatatt tattgcggat agttgggatg
     4981 tcacagaata tgcattgaaa gatgatgtgt tttctcaagt gcaaattaac aacatagtcc
     5041 ttgatagaga atttctagaa agtgatgtta tctatataaa atacgcagat caacaaatta
     5101 ggcaactagt cgatgcgtat tatcaagcgt atgggaaact catttctagt gccatgaatg
     5161 tttacaagcg ctctaacgct cgtagatacg tactgaaagg gaatttattc cgaccgcaag
     5221 acaatacaac acaagatcaa atcaataaaa tgatgacatc acaatttaag gcttttatgg
     5281 aagctgataa tgcaggtgcg gtatttcaat tacaaaatga gtacacatta gaagatttca
     5341 gcggaaactt ccaaagcaat tcaagagata taaaaaactt aatagacgac atctttgaga
     5401 tgacagcagc agcgtttcac gttccgaaaa acctactaaa gggagacatg agtgggttat
     5461 cggatcaagt ggacgctttt ttaatgttcg aaatcatacc gattgctgaa cttattcagg
     5521 atgcgtttaa cgctagtctc tatgaagtag aagaatactt gtcagggaat tttgtacgtg
     5581 tggatacaac tatgatcaag attactagct tcaaagattt ggttgacgct attgatgtag
     5641 gcattagaaa tggagtattt acaatcaacg aaggaagaga acgcgttgga aatgatcgct
     5701 ctgataaggc gatggcagat gaaatattta taactaaaaa caatcaacaa gtatcgaaag
     5761 gaggtgaggc gaatgacgac aatgaaaaca tttctagcag taaagaatga aggcgcagta
     5821 ccgcaaattt ttattcaggg atttattggt tctagttggt tctttgaagg gaatactgac
     5881 aagggaatca aaaatatttt ggatagtcta ggtgatcaag aagaaattga agtagtaatt
     5941 aattcaaacg gtggagacgt atttcaaggg attgctattg ggaacttact taagtcaaat
     6001 aaagcaaaag ttaacgttgt gattaacggc ttagccgcta gtgctgcttc aattatcgca
     6061 atggctggcg atactataaa aatctacaac aatgcacaat tgatgattca ccgcgcttcc
     6121 acatacggag aaggaaatgt cgatgacttc cgtacgattg ctgaccaact ggaatcaatt
     6181 gataaatcgg taaaggcttc atataaaaca cgattcaatg gcacagatga agcattgcaa
     6241 gaacttcttg aaaaagaatc gtttatggat gcagaaacag ctttgagtta tggattggtc
     6301 gatgaaatta tcgatgcaga aaatagctca ggtactgaag ctaaaaaaga acaaagcgtt
     6361 gaagaaattt tgaatgacgt taaagaaaaa agagcagaaa aaattgctgc atttacagca
     6421 gcattaaata aaacatttgg acaaggagat gtaaaataat gacagttaaa aatttaaaag
     6481 gtgtaacagc tgcaagcgac caattgatga aagcttttaa agatggtaac gaagaatctt
     6541 ttagcgcagc tatggtaagc ttatctaagg aaattcagga taaaatttta gaagaagcaa
     6601 cagcaaaaaa tcaagatcaa ttagtattaa tgaaccgtgg tcagcgtgta ttaactacac
     6661 aagaaacaaa attctataac gaagtagtga aaaacgaagg ttttgcaggg gtcgaagaat
     6721 tagtgccagc tactgtattt gaacgtgtat ttgaagattt agaacaatct catccactat
     6781 tgcaaaaaat tacttttgtt aacacaactg gtgtaacaga atggattgtg tcacgtggag
     6841 tcaatccagc atggtggggt aaactttgcg aagctgttaa aaaagtttta gataatggct
     6901 ttgatgtaat taacatgaag cagttcaagc tatcaggtta tattcctgta tgtaaggcaa
     6961 tgcttgattt aggtccagta tggttagatc gttatgtccg tactgtttta gtagaatcat
     7021 tgagaattgc attagaacaa gcaattgttg atggtactgg taaagatatg ccagtcggaa
     7081 tgatgcgtga catgagcaaa caaactagcg gagaatatgc tgaaaaaaaa gcagaaccta
     7141 ttacagcttt agatgctgta actatgggcg gtttgatggc gcgactatca aaattcaata
     7201 tcgaaggtgt gaatgatccg atttatcgta atgtgaatcc ttctgatgtg gtcctaattg
     7261 tgaatccaac tgattactgg tctaaagtat tcccagctaa gactgtacta actgctaatg
     7321 gagaatacgt acaagtattg ccagtaccag tttcagattt gcagtcaacg gctgtgccag
     7381 aaggaaaagc agttattggg gtagcctcag attacttcat gggtgtagga tctacactaa
     7441 aaattgaagc ttcagatgaa taccattttg ttgaagacga acgcatttat ctagctaaac
     7501 aatatgcaaa cggtcaacct aaacgtaacg atagtttcat tgtattagat attagcgctt
     7561 tgggaactac tactacaact acaaaaccaa caaccacaac aactacaaca caagcgtagg
     7621 tgatcagaat gaagtatatt ctttgtcagc cggcaatcaa tcggtttaaa tgggagcttg
     7681 aagtttgttt aactaatctg aagaaactag gaatcaaaga tatcgtattg cttttcagca
     7741 gacacgatga tcagattcct attttttttg agaaggaata tggtgttgaa gttcatgtgt
     7801 acgatgatct gcgggacgac aaagagtata ttccttcgat taaaccatat ttatggtgga
     7861 aatatttaga agaagatcat tcgcgtgagg acgaccgata tttctatatc gattcggatg
     7921 tcattttcaa taaaagaatt aatttgcgca aattgccttc taaagatgat gtttggtatt
     7981 gtagcgactg ctgtagttat ctaagtcttg attatattag aagctgtgaa aacggagaaa
     8041 atattctaaa agatatggca aacattgtaa atgttacagt agaatctttg gaaactataa
     8101 acactaattc aggaggcgca cagtgggtta ttaaccgtcc taaagcgaat tattggaaaa
     8161 aggtttatct ggattctaat cggctatatc gctaccttag agggcaaaaa acaaatatac
     8221 aaatctggac agccgagatg tgggcacagc tttggaacat gatgtatttc aatattggtc
     8281 ctaaagttca cgaggaatta gacttttgtt ttgctactga tccaatagaa aaagttaaag
     8341 aagtaaaaat cttacacaat gctggagtaa caacaaacga tgaagattta tttttcaaag
     8401 ggagatacgt gacttctacg ccttttgatg aagatttatc atttgtaaac aagaaaaaat
     8461 gctcttacgc atatgttaaa gcaattaagg cggtggttag atgacgcctg aacaagtgac
     8521 tgaagaattg ctaatagctg tgaaggataa tatttacgtt acctggaacg aagaagatga
     8581 gtcaattaaa aagatgatag ctaaaaatgc tgtttatctt caaagtaaag tgagtacaac
     8641 actttctttt tctcctgaaa gcttagaata cggattgcta atcgaaagat gtagatacga
     8701 ctggaatcgt gctttagatg agtttgaaca aaatttcgct agtgagttat taggtttcat
     8761 tcaacattat gcgctacaag aatatattgc aggtgatgtg aatggcgaat aatcgtagac
     8821 tcgaagaaac attcaacgat ggttggttaa agattttgac gcaaactacc aaaagaaatg
     8881 aactaggaaa aaagattggt gtagaagata cagaaatcac ttctttaaaa tttagaaatc
     8941 tttccatgag agatagtgat ataacagcta tggatgcgat gggatcgaaa ttaactaaga
     9001 aagtaaagac tccatttcat ccaatcgcca agaaatttaa taaagatcaa tattttatcg
     9061 taatcgatag tatgcgttac aacgttatct atgccgatta cgataatttt tatatctatt
     9121 tttatcttga aagtgtgggt gaatatggtg attgataatt ctaaagaaaa agaacgttta
     9181 aataagcaaa tttctgctat caaaacttcc ttagaagagc attttggcct caaactcttt
     9241 caagactccg taggcgagga tgagctacct gatgatttta attacttcat tctcgaaaca
     9301 ggagaaatag aaatgatcac tgagccaaaa tatagcgtgg gtcaaaatct atatctaact
     9361 ttctattcag aaaatagaga agatttaaca ggagactcac tagatattat ttcattgatt
     9421 caaaatcgtt cgattcgttt tcagagaatg gatcccaatc atttaaaact agagaaccaa
     9481 gatcgctata tcgatcaatt ggtatttacg tttagacgat tattgaagag tgattgtcat
     9541 ggctaaaaat agttgggagc taaaaataaa tggacatgat gaacttcttg tgcggatgga
     9601 acgctattca agcgagagcg aacgactgat taacgaagca ttgaaatcaa aaggttcggc
     9661 tattgcagtg gataggatta cagaaaaaat tcctgtttct gaagcagatt taagaagagg
     9721 gcaccaacac gcaaaaaata gtcgtccact taagactcaa tacattaatt tgggtttcat
     9781 cattagacct acaagaaaat ttgagtattt aaaatatcct gatttgggga taggtacttc
     9841 taaaagaaat cagccggacg aatttatgag aagaggatta ggtcttgcac ttgatccaat
     9901 tacagaactt ctgattcgtc aattcgataa attaaataaa tagggggaac aacaatggct
     9961 aaaacaaaaa ctgtagtaac aacgttcgat aacgtaagta tcaaacgaat tgcttttaat
    10021 tttaagaacg caggaaatgc aatcgcaacg aattgtaacg gacaattaga tggcgaaaca
    10081 gaaatgcaaa cggtggttaa aaaatgtgga gcgacagaag taaaatcaaa atctaaacca
    10141 atcaatatga cggtaacaat tactgcacat gtaccgatgg aagtttatcg acgtttcaat
    10201 gggttgaaac aagatggacg tattaaacca ggcatttact cttacggtcc tgattccgta
    10261 ggcgaagatt tctcacttgc tgcagagatc gtggatgact tcgaagaaaa tagaaagtta
    10321 gttggtatgt tagcatgcac ttcgaataca ggattaacat tctctattga aaatggtgcg
    10381 gatgaagtag ctgcgttaga actagaaaca aaagttatgc aagatgaatt tggtaaattt
    10441 tatcatgaag caattgttgc agaacttgaa gaagacttaa cagatcaatg gatgacgaat
    10501 ctatctgctg atgtgattaa aaaaacttca gttgtgacaa ctacggccac tcaatcacag
    10561 taaaaaaaac ggaggtagcg aaatgaacga agattactca aaaattgaac taaacgatgg
    10621 aacaattttg aatttagaac ctaaactgaa tatcaagaaa ttattgatga tcaatagaga
    10681 ttttaacaca gacgagtttg caaaaatgac tgtgggaaaa ggatccatgg atatttctgt
    10741 tattcaaggt gcaaaggctg tgtatattgc ttaccgccaa gcgaacatga ctgattatat
    10801 ttcattcgat gaatttatcg ataaatggga ttttgatatg gctactgcca gctatattta
    10861 tcaattgatg atgttcaaac aagcacgcga tgcttatcaa aaagaatttg aaaaagcaaa
    10921 taaggaaaaa aagcttcaaa agtaaaaatt ccaaagctct tagttgaaac gtgggtcgat
    10981 gtctattcga tgttgaccga cgttttttct atgccttcag atttggtttt aagcgatatc
    11041 tgtttagatg acattttgca aatggcttac aacaagagtg cttatgaagg atggaaaaac
    11101 tatgcaataa accaatccca gaaaaactaa agaaaggagg taaaaaatgg ctaaaaagag
    11161 aacagaagca gaagtaactt tcatagctaa cgatgacgga ttgaaatcta cgttaaaaga
    11221 aatcagtgct gaattaacta aaaatagagc agaattaaaa ctagaacaag ctcaattaca
    11281 acagactggt tctgaatcag acaagttagg aagtaaatta tcttctttag aaaagcagta
    11341 tgaattacaa agtcaaaaag ttgaagtaac tagccaacgt ttagccaatg cgaaaaaata
    11401 ttatggagaa aattccaccg aagttaagaa acttgaaaga gaactgatta atcaacaaac
    11461 agcgcaacaa cgtttgtcaa acgaaattga taaaacgagt aatgcactag ctcaagcaaa
    11521 aggcgaaata cagacgtacg agtctacaat gcaacagttg gatagcgaac aaaaaaatgt
    11581 tcaagctagt gcttctctga ttgaatccga atacaaaaaa tggcaagcaa ctgctggtca
    11641 atcagcttct gaagccgaga aattagcgaa agcccaagaa tatgtttctc aacaatctga
    11701 aaatgcggag aaaacgatag atatcctaag acgacagtta gaagctacac agtctgaatt
    11761 tggcgctaca tccacagaag caatgcagat ggaagcgaag cttaatgatg ctgaacgtga
    11821 atttgaagag ttaggacaag ctgctaaaaa tgtagataca actaacttgg acgatatcgg
    11881 aagcaaaata gatatgaata atttaatgga agcttctgac gttttaagcg acattggcga
    11941 taagcttaca gaattaggga aacaagcagt ggactctgct aacagtgtag gtagttccca
    12001 gagtaagata caagctaatt ttggtttgtc taaacaagag gctgaagaat taacgaatgt
    12061 agccagagac atttattata aaggttttgg agaatcgtta gatcagtcca cagatgcatt
    12121 gattttggta aagcgtaatt taggagattt aaataatcaa gatttacaaa atatcacgga
    12181 acaagctatg gtcctagaaa acaccatggg cgctgatatg gatgaaacgt tacgtggtgt
    12241 aaatggctta atggtcaatt tcggcttgag tgctcaagat gcaatggatt taatggtttc
    12301 gggtactcaa aacggtttag ataaaacgca cgaattaggc gacaatatgg cagaatatag
    12361 ccaattatgg agtcaaatgg gatattcagc tgatgaaacg ttcggaatgc ttcaaaatgg
    12421 tttagatgcg ggtgcttata accttgataa agtcaatgac ttagttaagg aaatgggaat
    12481 atcgttaaca gatggtcgat ttgagcaaaa catggatatg tttagtgaaa gtactagaaa
    12541 agcttttgaa gagtggaaaa atggcggagg aacacaaaaa gacgttatta attccatgat
    12601 tcaagatttt agcaatatgg atggtcaata cgaccaatta aataaagctt cgacaatttg
    12661 gtctgcgctt ggcgaagata atgcgatgaa agttgtccaa tctttaactg atgttaacca
    12721 tacatttgat gatgttagtg gatctgcaca aaaaatgaat gaagattcta ctactccgtt
    12781 gcaagagttg aacgggaaaa tagctgaatt aaaggattca ttagctccta taggcaacac
    12841 aatcatagat gcactcgaac cagtaattga ttttctagga aagatggctg atgcgtttaa
    12901 taatcttcca caaccagtac aggatttcat tgttgctata ggtggtctta cagcagcatt
    12961 tggcatactg ttaccggcaa tactagccgt gtcatattta tttggtccga tgatgcttat
    13021 aattggagga attatagctg ttatagcagg cgttattgtg gcaattaaga actggggtgc
    13081 aattactgac tggtttagtg gtttatggaa aaaatttact gattggttgg gtgatacttg
    13141 ggaaagtata aaagacggag cctcatcagt ttgggatggg gttaaagaaa cctggtctgg
    13201 atttgtagat tgggttcaag atatttggca aggagtttct gattggtttg gagagctatg
    13261 gagcggatta gttgaaggag cttccaacat ctggcaagga gtccaagaaa cttggcaaac
    13321 attcgttgat tgggtttcaa atatttggaa cggagtcaaa gaagtatggt cgattatttg
    13381 ggcagacatt gtaggaattg ttcaaatacc atggacatta ataacgtcat tgattcaagc
    13441 tggtattaat attatcgtgg gtatttttga tgtagctgga cagttattgg gcgcagcttg
    13501 gcaagctgtt tggacaccta tttctgattt ccttaaaaat acttgggata ctatgacaca
    13561 atggataagt atcgcttgga atgggattgt aactacattc catactatat ttgatccagt
    13621 agtggcatgg tggaatggta tatggacagc tattagtact acggcttcaa atatttggaa
    13681 ttcaattagt gcaacagctt ctagtatttg gaacagtatc aagaatacaa tcactagctt
    13741 ggtacaagca gctgctacag taattcaaaa tatttggtca actgtatcta gttggttagg
    13801 tggaatttgg aattcaatca gctctacagc atcaaatatc tggaatagtg tgactagtag
    13861 tataagcaat gctataaacg cagctaaaag tgccattcaa agtgtttgga atagtatatc
    13921 ttcgtggatt agcggaattt ggaacggtat caaaaatact gctttgaatc tttggaatgg
    13981 aattacaagc actattagct ctaaagtaaa cgatggaaaa aatgcaattt caagcggttg
    14041 gtccaatcta acaggtattg tttccgacat attcaataat gttaaaagta caattgctaa
    14101 catatgggaa ggcatcaaaa agactgttag cgctccaatt gattggatta gagacaaaat
    14161 cagtggcatt tttgataatt tgaatatttc tataccacat attccgttac cggaatttat
    14221 catggaaggc agctttaacc cgctaaaagg tcaaatcccc catttgcgtg taaaatggca
    14281 tgctaaagga ggtatcttta ctaaaccaac tttactaggt ggaatgaacg gtgtcggtga
    14341 agcaggacct gaagcagttt tacctttgaa aagatctgtt ttgcaagaaa ttggtgatcg
    14401 tatcttgagt agcacatcag tttcatctag ggcacaaacg attcaacctg tgaataacta
    14461 cgaattcaat ttcacaattg atggtaacgc agatgaggtt actatgaagc aaacaactca
    14521 acaaatcatt gatagcatta caaaaattca aaatgataat gcttcggcat ggcgttaaac
    14581 aggagagtat ttctcctgtt tttttagtat taaaaaggat gtgaaaaaat gactgattgt
    14641 atacattcta taatcgatgg atttcctgat tatttgcata aattggcttt agctgaaaga
    14701 ccaaccatac cttctccaaa aagacagaga gttgaaactt ctgttttagg taggttaggt
    14761 ggcttagtac aagattactc gtttgaagat atgtcgttta cattgcacta taactattta
    14821 gaggatgtgg aagaccatca agcgttcaag caatcgtttt atatcatgcg tcattggtta
    14881 aactatgcaa agaaattaga attctctgac gatcccaacg tctattacgt tatccagact
    14941 atcgatattg gggatgcaga aaacgatatt gttgaatggg gagagttcga tgtaaatatt
    15001 actgcgaaac cattcgcaag agttcaagaa gatgtaccta taaccgtaga taaaccacag
    15061 tcatttaact tgctgaataa tagtttagaa gaaagttttc caaagattat catcactcct
    15121 tcagctactt catgccagtt catcttaaat gattatgtgt ttagtttcga aggcttagta
    15181 gcaggaactg atgtagtcat tgatagtgat ttgatgcttt gctacgaaga gcaatcggac
    15241 ggagatattt tagatcggtc caacaaaatg aagaccatgc aatatccgac attgcaagtg
    15301 gatatcaatc attttaattg cactggtttg agcaaaatac aaatttatcg taacgggtta
    15361 aggtaggtga aatagatgat cgataattta ataactattt acgataaaaa tgacgcgaat
    15421 aatttagctg aacatttata tgatacgcaa ggtttaggcg ctttgtcaga ctggttaaca
    15481 gctactgtta gcaataaact aaacggagcc gagatatttc agggtactta ttcaataaac
    15541 ggaactaatg cagatttgat tgtagaagga cgtattattc agtgttatgt agatgaaaat
    15601 cgagcaaaac agcgtctacg gatttattat gcaaaaactt ctgtaatagg caatacgata
    15661 gaagtaaaag ctgaacctat tttcaatgat ataagaaaat cggtgttgaa taaatatgac
    15721 agcggaacag aaaagatcac tgctactcag gcatggcaaa acgcaaaagt tttagcgaaa
    15781 ccagctatcc cttcgcagtt ttctttctcg tcattagtag atacgcttgc taatgtgaag
    15841 atagaaaagg cgaatttttt agaattcttt ggtggaaaag agggatctat tctagatcga
    15901 tttcatggtg agtttctaaa agataataac acattacgtc atgaaaaaag tctaggtacg
    15961 gatcataaaa tcaaagcgat ttatactaaa aacttaactg gtcttgactt agagattgat
    16021 gctcaaagtg ttttagttgg agtttatcca ttcattagca gctcttcaga aggagaagac
    16081 gagatcactc taccagaaga agttattttc acggattacg tggatgatta tcctgctgga
    16141 tatgtttctt ttgttgattt taaagacaaa gcgactgatg tagccacatt aagggaagct
    16201 gctaaagact ggttgaaaac aaacatagat aaacaaaaac cacaagtgag tggttcgatt
    16261 gaattagtac cattgaggca tcaaagaggc tatgaaaaat ttgttgatct agaaaaagtt
    16321 tcgatgggtg acggagtaga tgtgtatcat ccacagttaa aagtgaatat gtcagcgaga
    16381 attgtggaat atacgtttaa tgttctaacc aattcatacg ataaattagt tgtaggaaac
    16441 gtcaaaacaa acttcttaga aaacacagag aataatgtca gcaatttgat taatgatgcc
    16501 attgatcaat tgaaaaacgg tggcgaaatc agtgatttac tcaatgatat tgtagatcat
    16561 caaactgata tgattactgg tcaaaatggt ggttatgttt tattagatcc taaagaagcg
    16621 cctagtcgta ttttgattat ggacacacca gataagaata ccgcaaggaa tgttttacaa
    16681 atcaacaatg ctggtattgg tttttctaaa actggcatta atggaacata tgaaacggca
    16741 tggacgttag atggcggatt caatgcctcg tttattacgg ctggtgaaat agtaggaatt
    16801 actattagag gtactacatt aattagtgat ggtgctgatt atagaacaag tattgctaat
    16861 ggcaaaatga cttggtactc aaaaaaagtt aacaaagata ttatggagct agaagcacgt
    16921 gattatgtaa gtgctgatgc cggtattgta tcatacacca tgaaaactgg tggtggtttc
    16981 atgattagaa atccacaggg taacttggtt tttagtacgt gggataatgg taataacaga
    17041 ccgtttctat cttttggtgc gcccaatttc aggtatagca atgctagtta tgtaacttct
    17101 ggcgacggta gttctttaag cattaatggt agtgcgggta actcatggga atttaaggta
    17161 gctggtagga ctatgaaatt tactagtgat ggtatgctaa cgttaccagg ttgttttttt
    17221 ggttcatggg aagatgggaa acttgctagg tttgaacaat caacggtaca agtatataaa
    17281 gattttactg ttagaggtac taaaaactca actgtaccaa cagaacatta tggacaacga
    17341 ctattgaacg cttatgaaac tccagaatat tatttcgctg attatgggga agccgttaca
    17401 ggtgacaatg gtaaagttcg tgttgatatt gaccccatgt ttgctgaaac agtaaatcta
    17461 agtcggtata tgacacatgt gacacctaca gaactagttt tgtgtgctgt tactcatgaa
    17521 gatattgacc atttcatcat tgaaactagt aagccaaacg tattagttag atggaattta
    17581 gtggcacacc gtctagggta tgaagatatt agattaaaag aggatacagc atatgatagc
    17641 acagtgcttg accaaaaacg tttttaaaac gaagacaagg aggtatataa atggctagca
    17701 gtttatataa tttggcttta gatttcagca aagaattaaa ctacaccaaa gctattatgg
    17761 ctcgtcaagg tgataaaggg attacggtga cggttaaacc gtttctaaat ggcttgcaga
    17821 tggatacgag tggcggaaca tttactttaa aaggaacaac accatctaac cgttacgtag
    17881 ataatgttgc aactagtgta actagtgaag aagtcacgtt ttctcttgat ggcacattta
    17941 tgagtgaagc aggatattat aaacactgct acgtagaata tagaaaagac aatcaaattt
    18001 taacaacgca agatatcatt tttttctcac taggagtgtc tgacatttcg caaggccaag
    18061 ccgatgaata tgtttcgcaa ttagaagagt tgattcgaaa gtacaacgaa acttttgatg
    18121 cttttatggc tgaaatcaaa ggtagagtgg atagcttaaa tcaacagatt actgatttaa
    18181 ctggtcaagc taaaacgcta caagacaagt tagatgctct gaaagaagaa atttctaagt
    18241 taggtaactt acaagtgatg tacagtaaca gcatcgactt cggggactat gattattcgg
    18301 agaacccgaa tttaatgcca tatattacag aaccttgggt tggacctttg ttaggtaatg
    18361 gacatacagt taaagatagt gttaaaaggg ttataactca tactaaaact agaacagcta
    18421 attccggcga catattatcg cttggcttag gtattccgtg tacagcggaa gctaataata
    18481 ggtatcttat aaccacttta cgaccatcta cgacatacac attaagcgtt actatgagtg
    18541 tcggttcaga ttggactgga gagaccaata ctataggggt taggcttaga tatttaaatg
    18601 aacaaggtgg tatagaactg cccattaatg cattaatacc agcaaatgta gaacgcgata
    18661 aaatggtcac tcatacattt actggtataa caaaagataa tgtaactagc ataacaaact
    18721 gttatgttga aatattctca cttaatagtg aatataaagg aacggttagt gtcagctatg
    18781 acgttaagtt aaaagcacac taccctaact tactagatgg cccttactgg ttaggtaaag
    18841 ttcctttagg cgagaatatt gctgacccta cagttgtctt tccacacaaa actagtgaat
    18901 atatggttta tggtagacgc aatacagaaa actatatcgc tgaccaaaca tataccatat
    18961 caatgaaagc aactaaacta acagttcaat cgtttgccgt gtatattgct gctggccgcg
    19021 ttaaagtagg agacatgaag cctactgagg ggttagctaa tacatgggag ttaactttta
    19081 ctgtaactaa gcaacatata gatagtgggg taactaatta tctggaaata taccaatacc
    19141 cgtcagctac aaaaggagct gttcaaattg agtggctcaa actagaaaaa ggcaatactc
    19201 gaaccccgaa tattagtgag tataaatatc gtggtactgg tatgcgtgat tcaaacaatc
    19261 caaaagatta tgtttgggat ctagcaccag aatatgtcga agataatttg gccacagata
    19321 ttaaaatttc tgaaattact ggtaaagcaa acaattatac cgatgggaaa gtatcggaga
    19381 ttaattcgca gttgactgct tcaattaatg aagtagacac cacagctaag gatgctcaaa
    19441 caaaagcgaa tgctaatgcg actgctatag atgaattaga caataagatc gatgaacgca
    19501 ttaatgatac agctactacc acattaacag ttacaaacgg gaataccgga tcagcaaagc
    19561 tttatcgtga aggaaaaaca gtttctatat attttgtggc tttaaacgga aaaagcagtg
    19621 gtggaaatga ttcaacgata ctaacaattc cagaaggcta tcggccacca attagttttg
    19681 agcaactggt tggctcgata gaccgttcta ctttgaacag tgctcagtta tctattggtg
    19741 cagatggagc cattaaatgg cgaagaaact caagttatgg atcggattat acctttgcaa
    19801 ttacttacac gatttagaaa agcgtgaatc gatatgaagg cagcatatag accaattgaa
    19861 ccttacggat tcgagcaaat cattgtgaat gatgaagaac atttaccgga agaatgcaca
    19921 gaagtcgaac caccgattcc aaattggaaa ccgaaattca attactggga gggaaataaa
    19981 tgaaaaacat ttggaaatat ggacgtactg gcggagagta cgcaggaaaa gtattggacg
    20041 acatgcttgt atccgttcct tacacagatc agccaccgct tgaaggaatt cgtgctgatg
    20101 gcgaaccgtt aacaatcgct gatcaaatgt ttgatcctaa attgaatcaa tggattattt
    20161 tagcgaacgc actagatcac aacgatttaa acaatctcaa agcaatgtat gagtcgttag
    20221 aaaatgagaa cggcgattta aaacagatca atgccaaact catgctaagc gatgtagcaa
    20281 ttaaacagga aaatactgca ttgaaagaaa aagcggatag tttagcacaa atcaattcaa
    20341 aaatgatgct tgcttcgtta caaaatagca aagacatttc agaaattaaa gagcaactaa
    20401 atccagcttc aaagggaggt gagtagtatg tttagtttta gcgatgtgaa aatgatgtat
    20461 gattggggct gtttcactaa cgaacaagta atggttttcg ttccgttgtg cattactgaa
    20521 gaa
//
LOCUS       DAFBQH010000046        20285 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.048, whole genome shotgun sequence.
ACCESSION   DAFBQH010000046 DAFBQH010000000
VERSION     DAFBQH010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.048"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            140..460
                     /locus_tag="LK694_001786"
     CDS             140..460
                     /locus_tag="LK694_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052635.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="LK694_001787"
     CDS             912..1112
                     /locus_tag="LK694_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK5052636.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="LK694_001788"
     CDS             1259..4048
                     /locus_tag="LK694_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBK5052637.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="LK694_001789"
     CDS             4095..4622
                     /locus_tag="LK694_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HBK5052638.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="LK694_001790"
     CDS             4643..5833
                     /locus_tag="LK694_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK5052639.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="LK694_001791"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="LK694_001791"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBK5052640.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8224..8898
                     /locus_tag="LK694_001792"
     CDS             8224..8898
                     /locus_tag="LK694_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBK5052641.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8895..9236
                     /locus_tag="LK694_001793"
     CDS             8895..9236
                     /locus_tag="LK694_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052642.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9266..9625
                     /locus_tag="LK694_001794"
     CDS             9266..9625
                     /locus_tag="LK694_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBK5052643.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            10149..12230
                     /gene="cadA"
                     /locus_tag="LK694_001795"
     CDS             10149..12230
                     /gene="cadA"
                     /locus_tag="LK694_001795"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBK5052644.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            12541..14007
                     /locus_tag="LK694_001796"
     CDS             12541..14007
                     /locus_tag="LK694_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5052645.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            14622..15161
                     /locus_tag="LK694_001797"
     CDS             14622..15161
                     /locus_tag="LK694_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HBK5052646.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            15235..15675
                     /locus_tag="LK694_001798"
     CDS             15235..15675
                     /locus_tag="LK694_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBK5052647.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            15688..15897
                     /gene="copZ"
                     /locus_tag="LK694_001799"
     CDS             15688..15897
                     /gene="copZ"
                     /locus_tag="LK694_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBK5052648.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            15897..18083
                     /locus_tag="LK694_001800"
     CDS             15897..18083
                     /locus_tag="LK694_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBK5052649.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            18103..20274
                     /gene="copB"
                     /locus_tag="LK694_001801"
     CDS             18103..20274
                     /gene="copB"
                     /locus_tag="LK694_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HBK5052650.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
BASE COUNT         6777 a         3526 c         4407 g         5575 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaacggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ctatcatcat
     5341 caacggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa
     5401 tcctgtgatc atcaatggaa tgatcgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag caagatacag tggaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatattcg cttgtggcag aactgccggg
     5581 tgtttctctt gaaaagccac aaggagcttt ttatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctaaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatat gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttatgatga
     6721 tgcgatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtgat
     6901 ctgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacagactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggtgatg aaaaagctgt tctgcatcaa
     7201 gtaattagaa cagccaagca aaaacagctg ttcatgtttt tgaattggcg ggacttatta
     7261 ccgcagatgt aagcttttga ataccgttta gtggagaggt tgtgaaaaag ctgtttagtt
     7321 tcgagtatta agaaaaattt tgtggaaatg gctttccaca tttttgcata aatttggctt
     7381 aataccgaga aactggcttt tgaacaccgt ttaaggaaga ggttgtgcgc ctgccgttca
     7441 gctccgagtc acaaggagta atttcaaaaa acagtttttc atgtttgatg actattgtga
     7501 cttgtgaccg aggagttggc cggagagcac cgtttattct aaaaaagaac tgcgaactct
     7561 cgcagttctt tttttaaaat acgggataaa ggaagtttcc agcagacaag agaagaaagc
     7621 aaaagaaatt gctatttgat tcacaaggtt ctataacgaa atcgcaatca atcaaacatt
     7681 tacaaagaag gtaattcaaa tggaaatctg atttgatgat gcttctgctg ttgaaataaa
     7741 gcagcatcta gcacctgaaa acaaactaat agtaacatta gaagatggag taggtccgta
     7801 ttttcagcat gcaataatcc atatgtaagt tcaattcacc cttgaaatca tcggaccaga
     7861 taaagttatg accaatctat cccaagcaat ctagaagaga tattagtaaa gggacattta
     7921 ctagaagtct tagatgagcc tataaatata tgttctgatg gcgtcatcag tggtctgatt
     7981 gacggaagct taggtgtgat cgatctctct ggaccaaaca acgaaggaat caaggggaat
     8041 ccggcacgtt aagaaaaaaa tagcataaaa aactagacac ttgatgagcg ttcattcaat
     8101 tgtttgaagg gactaggctg gtgtcatttt ttatacatat attttctctt tcgtcttgat
     8161 agggcttatg ggataatata aggaagaaaa tcatcgtttt attcaaaaag gagtttttga
     8221 caaatgaaaa aacgtatatt atttatcggt gatagcatta cagacgcagg aagagaccga
     8281 gcagaagcaa ctgatctagg taaaggttat ccacttttga ttgccggaaa actaatctcc
     8341 caatatccaa cgatggaatt tgaatgcatg aaccgtggga taggaggaga caaaattgca
     8401 gatctgcaag tacgctggca agaagattgt ttagctttac aacctgacta tgtatcgatt
     8461 ttgatcggaa tcaatgatac ctggcataat actagcgatc aggctatatt cggaactgag
     8521 aaatctgctc agcaatttga atccgtttat cgaaaattac taaatgaact tcaaacagtg
     8581 ggaatctgca acatcatctt gatggagccg tttgtacttc ctataaccga cgaccgtcat
     8641 ggctggcgta aagacttaga ccctaaaatc caaattgttc gtaaacttgc taaggaatat
     8701 ggcgcattgc tggttccttt agatggtata ttcaatgctt tagggattgt ttatggtttt
     8761 tcgaaatata caagagaaga tggggtccac ccaacgattg gcggacatga gatcattgca
     8821 caaagctgga tagatgcaat tgccgcagtc gattttttta gtggagataa aaatgagcga
     8881 aagaaaagtg agaaatgatg gaaatacggt gggcagtaaa agaagatcta cctcaaatca
     8941 atcagctgat tactgatcat ccagaatcat tcgtaaaagc aaaaacggca gctgacctaa
     9001 atgataagaa taaactattg ctcgttgctg taacaaaaga aaaacggatt atcggatttg
     9061 cgcaagcgga cgtgcaacag tcagaagaac gattggatca gcgaccatca ggaaaactaa
     9121 tccaacacac attagtaaca aaagaaaata cggaagaaat ccaaaaaaaa tttgcagaat
     9181 atttgatact ggaagctaaa aaaagaagaa taaaatcatt ttttgcccag ttttaatcaa
     9241 aaacagtaat agaaggagaa atacaatgag ttgtttagga aatgtcatct ggtttatatt
     9301 cggcggacta atcggtggtc ttagctggtt agcagcgggg atactttggt gtatcacgat
     9361 cataggtatc ccaatcggta tgcaatgttt taagatcgcc tccctttctt ttgcgccttt
     9421 tggcaaagaa gtaatttatc accagtctgg catgtcattg attgttaata tcatctggct
     9481 gatcgtttca ggcctaccaa ttgctctcgc acatgtcgtc agcggcatcc ttctatgtat
     9541 cacgatcata gggatacctt ttgccaagca atctttcaaa cttgctcgat tggcgttgat
     9601 gccattcggt gcagaaattg tataaaagag aataataaag gttagataaa gcatagagtc
     9661 aggtgggata catgtatttc acctgactct atgcgttctc ctgtataagt tcttccatgt
     9721 gacttttacg tcttttattt atatggggtt actgtttatg gaaagttgaa ataattttca
     9781 ttaaagaact ctttctatat ttttgaaagc gtagtggagc ttcgaatatc tgggaaggac
     9841 attttgcagg gattgaaaaa agatattgtc ctatcgaaaa attaatgaat ttagacaaat
     9901 tgccagcaac aggcttcaaa cttagttgct atcctgtcaa gatcaagaag gcaagtgcgg
     9961 gttggataag agcagtggca atgatcgaaa agaaaaagaa agaataagcc aaaacatctg
    10021 tatggaagaa attttccgat gcagatgttt ttcatttttt ctttcggata ccttgacata
    10081 tgaataaccg ttcatttata attagttatg aagagatatt catatgttga ggcgaggagg
    10141 tcattaaaat gaaaagttac agattagaag gtttagattg tgcgaactgt gcaatgaaaa
    10201 tcgaaaaagg agttcagaag atcaatggag tcaaggaagc aacggtaaac ttcacttctg
    10261 gaaaactaac gattgacgca gaggaagatc atttggctac gattgaacaa gaaataaaaa
    10321 aagtagtgaa ggaattagaa ccagatgtca aagtaacaga aatagataag gaaaaagtat
    10381 ctgaacatgg aaatgaaaaa gagagaaata cgctgttccg gattttattt tcccttgcag
    10441 gtatcgcttt attgctatta tttgatttta atgaaccaat tcgcttgata gggtatttat
    10501 tgatttatct gctgatagga tatgatgtag tgaagaaggc agtgatgaat atcgtcaaag
    10561 gaaaaatctt tgatgagaat tttcttatgt ctgttgctac gattggcgct atgttgatcg
    10621 gtgaatatcc agaagccgta gcagtgatgc tattttatca aatcggcgag tatttccaag
    10681 gattggcagt cagtcattca agaaaatcga ttcgcgaatt gatggcgatc cgtccagaaa
    10741 cagctcacgt gcagacgacg gaaggattga tgaccgtgaa ccctgaagac gtgcagatcg
    10801 gtcagtttgt acttgtgaag cctggtgaac gtgtaccgtt agatggaacg atcatcgaag
    10861 gagaatcatt agtcgatacg tcagcactta caggagaatc agtccctaaa tctgtatatg
    10921 ttggtgaaac agtattaagt ggatttatca ataaaaataa accattttta gttcaagtag
    10981 aaaaatctta tgaaaattct actatcagca aattattaga attagtagaa aatgccagca
    11041 gtaaaaaagc accagctgaa aatttcatta ctaaattcgc gcgctattac acaccagtcg
    11101 ttgtaggatt ggccgtttta ttagctgttt tacctccact tgttgtttct ggagcggcgt
    11161 tttctgaatg gatctatcga gcattgacat ttttagtgat ctcatgtcca tgtgcattag
    11221 tgatctccgt accccttagt ttctttggcg gtatcggagg agcaagtaaa atcggtgtat
    11281 tagtaaaagg ttcaaattac ttggagttgc ttgcacaaac tgagacagtg gtattcgata
    11341 aaaccggcac attaacgaaa ggtgactttt cgatccagac gatcgataca aaaatcgatc
    11401 caaagatatt catgcaatat gttgcgagtg cagaacagtt ttctacacat ccgattgctc
    11461 aatctgtttt agaaggctac gcaggtccgc ttttaccaac agctaatatc caagaatttg
    11521 caggtgaagg gatcttagct gaagttgatg gcaaacaagt attagcaggt aatcacaaat
    11581 tgatggaaag atttgaaatc tcttttcctt caagtcaaga aatcggtaca ttgctttatc
    11641 tagcgatcga tcaaagttac agtggttatc ttgttattgc agataccttg aaagaagatg
    11701 ccgtagacgc attggttcaa ttaaaaaagg ctggtgtgaa gaacacagtc atgttgacgg
    11761 gcgattcgaa aaaaattgct gatcatatcg ggaagcaagt aggcgtggac aagatatata
    11821 gcgaattgct tccagaagat aaagtgcagc ggttagagga aatcttacaa agaaacaata
    11881 aaaagaccgc atttgtcgga gatggaatca acgatgcacc ggtgctagca agagcagatg
    11941 taggaatcgc aatgggtgga ttgggaagcg atgctgcaat tgaagcagca gatgtagtca
    12001 tcatgaatga tcaaccatcg aagattgcag aagcaatcca tttagcaaag aaaacattga
    12061 aaatcgtcaa acagaacatt gtctttgcta ttgggataaa aattttggta cttgccttag
    12121 gtgcttttgg ttttgcatca atgggagatg cagtatttgc agatgttggg gtgacagtat
    12181 tagctgtttt gaatgccatg cgaagcttgc atgtaaaaaa taaacgttga tcaatgaaga
    12241 ggacgaatga acgtcctctt ttttttatat ccttttctta atgaaaaatg taaagaaaat
    12301 agataggatt ataaaataac taatgttttt aattttgcag aaaattcaaa atagcttgaa
    12361 attcaataag gtaaatgaaa taaaagaaaa taacttattt ttttgatgga aaaaatatga
    12421 aaaaacgatg tgtattaaat aaaagaatac acacataaaa taacactgtc ttgtaagttt
    12481 gttaaaaatt gaatatgatt acatctggat aacaaatttt tatcataaag gagagaacga
    12541 atgaacaaca aacaattacg ctggtttact gttgcgttaa ttgcatttaa catggtttgg
    12601 ggcatgggga atgtagttaa taactatgct cagcaaggag tatcagttgt cacctcttgg
    12661 ctattgattt tggccattta ttttattcca tacgcattga tcgttggaca attagggtca
    12721 gccttcaagg atagtaaagg aggcgtcagt tcatgggtcg aaaatacgac atctaataaa
    12781 cgactggcat actatgccgc ttggacttac tgggtcgtcc atattcctta tttggcacaa
    12841 aagcctcaag caatcctgat tgctggcggt tgggcagtcc aaggaaatgg aaatatcgta
    12901 aatacgatgt cagtacaaat ggtagcagtt atttcactta ttatatttct tgccttttta
    12961 tatttatcaa caaaaggact ttctactttg aaagtgattg gcggactggc cggaacagcg
    13021 atgttcgtca tgtcgatctt atttattctt ttagctgtaa cggcaccgtt catcaatcca
    13081 acgatggaat ttgccacacc acatatgaat caagtgaaaa catatattcc aacatttgat
    13141 ttcagttatt tcacaaccgt ttcgatgcta gtatttgcag ttggaggagc ggaaaaaata
    13201 tccccttatg tcaactctac aagaaatcct gcaaaggaat ttcctaaagg gatgatcttc
    13261 ttagcagcaa tggtagggat ttgtgctgta ttaggctcag tcgcaatggg tatgctgttt
    13321 gctagcgata atattcctaa agacttgatg gcaaatgggg catacagtgc ttttcaaatt
    13381 ttaggtaatt actatggtat cggtaactca ttgatgatca tttacgcatt gacaaacatg
    13441 attggacaaa tctccgcatt agccttttca attgatgcac cactaaaaat cttgttagca
    13501 gatgcggatc cagaatttgt accaaactgg ctacgtaaac gtaatcgtaa aggtacattg
    13561 gtgaatggtt atcttctgac aggtatacta gtaggaatca ttattttgtt gccgatcttt
    13621 ggtatcaaag atatgaatga attagtaaaa tggttgacga acttgaactc agttgtcatg
    13681 ccaatgcgtt acctatgggt gttcttggca tatatgatgt tgaataaagc ttataagaaa
    13741 ttcaattctg aatataaatt tgttaaaaat ccaaaaatcg gttttatctt tggtgcttgg
    13801 tgctttgcct tcactgcgtt tgcatgtatc ctaggaatgg tgccaaaagt agattatact
    13861 gctgatccta aagcatggtg gttccaatta ttcacgaata tcatcacacc aattgtcttg
    13921 atcttattag gtatgatctt gccagcaatt gctcgtcgag acaaaaacaa agaaatcact
    13981 gaaccagaaa cagtggttca gccttaaaat agaaatactt gttacaagag gttgtgacag
    14041 aagtggtcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    14101 aagtttcggc ttgtttttcg cagaagctac ttctggaaca ccgtgaatca gaggttgtga
    14161 aaaagctgtc ctgcatcaag taataagaac agccaaacaa aaatagcccc tcatattttt
    14221 cgttttgctg ggcttattac cgcagatgca agcttttgaa caccgtttaa gaaagaggta
    14281 gtgagcctgc cgtttaactc cgagtcacaa ggagcaattt caaaaaacaa cttctcatgt
    14341 ttgttgacta ttgtgccttg tgaccgaggt gttggcagga gaacaccgtt taaggaagag
    14401 gttgtaaaat cagcgatttg acctgagtat tagaggaaca aacgaaaaaa tagctcctca
    14461 tattttgccg ttttttgagt taatgctgaa ggtcagctga ttgaacacca tttattcgaa
    14521 acaagggact ttgacacgac ttttgtcaca gtcccttgtt tttttgcggc gttataagtg
    14581 gtatgatcga accaaattcc aaagagaagt gagggagaag aatgaagatc atcgtgattg
    14641 gacaaagtgg gtcgggaaaa tcgaccctcg caaggaagat caaagaaatc accgactttc
    14701 cgctactgcc gctagacctt ttatggcata caacagacta cagtattcag gcaaaaaagt
    14761 ggtttttgca agaacagcaa tcgtttatgg tatctaatcc gtcatggatt gttgaaggaa
    14821 attatacttc tacattagag gaaaggatta aagaagcaga taagatcatt tggcttaaag
    14881 tgccgcgtta tctcgccatc taccgagtag tttggcgttc tctaaaaaga aagataaaca
    14941 aaaaatctcg gccagatatg cctgaatcat tttcagagaa actaaacagg gaatacttag
    15001 aatttttatc ttttatctgg cattttgaag aaaacaatga accgaaaata cagcagctga
    15061 tccaagtagc taatgcaaga aataaactga tcatccttaa aacaagacga gagaaacaag
    15121 aatttttaac taaattgaaa atagagagca aaaataattg acagtttttg tttacaagcg
    15181 taatctgtat tcataagcta atgattacat ttgtaaacga aaggtgtgaa ataaatggaa
    15241 aaagtcaaaa cgccagtaaa gatcagtgat tcagagtggg agatcatgcg tgttatttgg
    15301 acacttggac agacgacagc ccaagaaatc acgcagatct tagctgattc gatggattgg
    15361 aaaccagcaa cgatcaaaac tttgctggga cgtttggtaa aaaaagaagt gatctggaca
    15421 gaacaagaag gaaaaaaatt catttatcat ccagctgtct cggagatgga aacagttcgc
    15481 agtgcgacgg aaaatctttt ttcccatatc tgtgcaaaaa ggattggaga gacgattgcg
    15541 gatctagtag aagaagccac tttgactcaa gaagatatca atgagatttt gaaacaactg
    15601 gaacaaaagc aaccagtaga tacgatcgaa tgtaactgta tcccaggtca atgtgaatgt
    15661 aaacaacatc aataggagga agaaaaaatg aaacaacaat tttcaatcga aggtatgagc
    15721 tgcaatcatt gtgcagcaag agtagaagaa acagtaagcg cacttgacgg cgtgcaaaaa
    15781 gtgaaagtaa atttgaaaaa agcaaacggt acggtcaagt ttgatgaaac aaaagttcaa
    15841 tccgaaaaga tttgtcaagc aatcaatgga ttgggttata aagcagaggt gatttgatgg
    15901 caacagcaga aaccaaaatg gatacgttcg tcattacagg tatgacttgt gcaaattgtt
    15961 ctgctaggat cgaaaaagag ctgaacggac aacctggtgt ggtcaatgcc acagtcaact
    16021 tggccactga aaaagcaagt gtcaaatatg aagggacgac cactgaaaaa ttaatccaaa
    16081 gcgtagaaaa catcggttat ggtgcaattt tgtacgatga agcccacaaa caaaaaattg
    16141 cagaagaaaa gcaagcgtat ttgaaaaaaa tgctattcga tcttatattg agtacggttt
    16201 tgactttgcc gttgatgttg tcaatgattg ctatgatgtt aggaagccat gcggcgattg
    16261 ttcacttttt tcattttcca atcgtccagt tagtgctgtc tgctcctgtc caattttatg
    16321 tcggtgctcg tttttataaa ggtgcgtacc atgcaatcaa gacgaaagca ccaaatatgg
    16381 atgtattggt agcaattgga acatcggctg cctttgcatt gagcatttac aacggatttt
    16441 tccgtggaca cccccaagat ctttattttg aaagcagcag tatgatcatc acgctgattt
    16501 tgctcggcaa atatttggaa catacagcaa aaacgaaaac aggtaatgca atcaaacaat
    16561 tgatgtctct tcaaacaaaa accgcccaag tgataagaaa cgggaaagaa gaaacgttag
    16621 cgattgaaga agtagtcgtt ggtgatcaac tagtcatccg tcctggtgag cagattccag
    16681 cagatggtcg aattatttca ggaagcagtg cgatagatga aagtatgctt actggtgaaa
    16741 atcttcctgt agaaaagaat ccggatgaca cgctttttgg cgggacaatc aatacgaatg
    16801 gtctgctaca catggaagtc acacaagtcg gcaagcagac agtattagcg caaatcattc
    16861 aaatggtgga agatgctcaa gggagcaaag ccccaatcca aaaaatcgca gatcggatct
    16921 cagggatttt tgttccgatc gtattagtca tcgcgtttat cacgctcata gctacaggtt
    16981 tgatcactgg tgattggcaa ctcgcattga tccatagtgt ctctgtatta gtcattgcgt
    17041 gtccatgtgc attaggttta gcgaccccaa cagctattat ggtaggaacg ggtgttggtg
    17101 cacgaaatgg aattttaatt aaaggcggag aagcattaga agcagctgct cacttagaca
    17161 gcattgtttt ggataaaact ggaacgatta cagaaggaaa gcccaaagtt acagatctag
    17221 tcggttcaaa agaagtccta tctatttttt atactttaga acaagcttct gaacatccac
    17281 taggtaaagc aatcgtggaa tacggaaaac tgcaagaagc agcaacatac gatatgattg
    17341 attttacagc ccacccaggt gctgggatca gcggtacgat taatggagta cgctattttg
    17401 cgggtacacg taaacgatta attgaattga acctatcatt tgatgaatac caagaacacg
    17461 cgttagaatt agaacagcag ggaaaaacag tcatgttttt agctgatgaa aagcaagtga
    17521 tcgggtggat tgccgtggct gatcaaatca aactagaagc caaacaagca atcaagcagc
    17581 tccagaataa aggattagat gtttttatgc tgacaggaga caacaaactg gcagccgaaa
    17641 cgattggtaa acaagttggt attgatccaa agcatatctt tgctgaagta cttccagagg
    17701 ataaagcagc ctacgtagaa aaacttcaga aagatggcaa aaaagtcggt atggcaggcg
    17761 atggaatcaa tgatgcaccg gccttagcat tagctgatgt cggtatggca atgggaagtg
    17821 gaacggacat tgcgatggaa acagccgatg tgacgctgat gaatagcagt ttggcatcca
    17881 ttgcacaaac aatcgagttg tctaggttga cattgagaaa aatcaaacaa aatcttttct
    17941 gggcttttgt ctataatacg attggtattc cttttgccgc actaggcttt ttgaatccaa
    18001 tcattgcagg aggagcgatg gcgtttagtt ccgtcagtgt attgttgaac tccctgagct
    18061 taaatcgaca catgaaaaag taagagaagg gttggagtaa aaatgaatgg gaacgatatg
    18121 gagaaaaaac atgagcataa aaaagaagta gtaaacaaag aaagtacaga atctcatgaa
    18181 aaacagatgg agatgaaaca tgaccatagt cagatggatc attccatgca catgggacat
    18241 aaccatggag atatagcaaa cagcaaacaa atggaccatt cgatgcacat ggatcacgaa
    18301 cacggcggca tggatcattc catgcacatg gggaatttca aacaaaaatt ttggctatcg
    18361 cttatcctag ctatcccaat cattcttttt tcaccaatga tgggaatgga atttccattt
    18421 caagtaacgt ttcctggttc tgattggctc gtattgatct tagcaactat actatttatc
    18481 tatggcggtc agccattttt aagtggagct aagatggaat tgaaacaaaa aagtcctgct
    18541 atgatgacac tgatcgcgat ggggattaca gttgcatata tttatagtgt ctattcgttt
    18601 atcgcaaatc tgctcaatcc acatacacac gtcatggatt tcttctggga attggctact
    18661 ttgatcgtta ttatgctact aggtcactgg attgaaatga atgctgtgtc gaatgccagc
    18721 aatgctttac aaaaactagc agaattactg ccagaatcag tgaaacgctt gacgaaagat
    18781 ggaaaagaag agacagtttc attgaaagaa gtaaacgaag gtgatcgttt gatcgtccgg
    18841 tctggtgata aaatgccaac agatggtgta atcttgaaag gtgaaacaat tgttgacgaa
    18901 tcagctgtga ctggcgaatc aagaggaatc aaaaaacaag ccaatgacaa agtgattggc
    18961 ggttctatca atggggatgg aacgatcgaa atcgaagtaa ctggaactgg tgaaaatggt
    19021 tatctagcga aggttatgga aatggtgcga aaagcacaag gagaaaaatc caagctagag
    19081 tcattatctg acaaagtggc aaaatggctg ttttatgtcg cattaattgt gggtatcctg
    19141 gcattcatcg cctggttgtt cttgacagat cttcctaatg cattagaacg tatggtaaca
    19201 gtctttatca tcgcctgtcc tcatgctcta ggtttagcta tcccattagt tgttgctcgc
    19261 tccacatcaa tagcagctaa aaacggatta cttttgaaaa accgcaatgc actggagcaa
    19321 gccaatgatc tagatgtgat catgctggac aaaacaggaa cactaacaga aggaaaattt
    19381 actgtaacgg gtgtcgaagt acttgatgat gcgttcaata aaaatgaaat ccttcaatat
    19441 ctaggagcct tggaagcgaa tgccaatcat ccattggctg ttggcattat gaattatttg
    19501 aaagaacatg aaatcaagcc ttatcaagct gaaaatctaa aaaatctttc aggagtcggg
    19561 ctagaggcaa cagtcaaaaa acaacaagtg aaaatagtta atgaaaaaga agttgagcgt
    19621 ctcggattaa acgtagaaca agcattattg aaaccttacc aagaacaagg aaataccatc
    19681 agcttcctga ttttagaaaa tcatctagct gcaatcgttg ctttaggtga cgtagtaaaa
    19741 acagaagcaa aagaatttat ccgaacattg aaagaaagaa aaatcactcc tgtcatgctt
    19801 actggtgaca ataaaaacgc tgcccaagca gtagcagatt atttagggat cgaagaatat
    19861 tatggcggtt tattaccaga tgataaagaa gctgttgtcc agaaatacct tgaccaaggg
    19921 aaaaaagtaa tcatggtggg tgatgggatc aatgatgcgc caagtttagc ccgtgcttca
    19981 atcgggatgg caatcggtgc agggacagat atcgcaatcg attctgcgga tgtcgtattg
    20041 acaaacagcg atccaaaaga tattttgcat tttcttgatc tagccaagca aacgcgaaag
    20101 aaaatgatcc agaacctctg gtggggggca ggttacaata ttcttgcaat tccgctagca
    20161 gccggaattc ttgcgccaat tggtatcata ttaagtccag cagttggtgc tgtcttgatg
    20221 tcgttaagta cggtagttgt agctctaaat gctttgacat tgaaaatcaa ataataatga
    20281 aaaaa
//
LOCUS       DAFBQH010000047        20038 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.049, whole genome shotgun sequence.
ACCESSION   DAFBQH010000047 DAFBQH010000000
VERSION     DAFBQH010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.049"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            68..751
                     /locus_tag="LK694_001802"
     CDS             68..751
                     /locus_tag="LK694_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBK5052651.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="LK694_001803"
     CDS             753..1589
                     /locus_tag="LK694_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBK5052652.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="LK694_001804"
     CDS             1600..3354
                     /locus_tag="LK694_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5052653.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="LK694_001805"
     CDS             complement(3630..3971)
                     /locus_tag="LK694_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBK5052654.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="LK694_001806"
     CDS             complement(4039..6210)
                     /locus_tag="LK694_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBK5052655.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGLTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6379..7278
                     /locus_tag="LK694_001807"
     CDS             6379..7278
                     /locus_tag="LK694_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBK5052656.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="LK694_001808"
     CDS             complement(7349..8206)
                     /locus_tag="LK694_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK5052657.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="LK694_001809"
     CDS             complement(8191..10893)
                     /locus_tag="LK694_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBK5052658.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="LK694_001810"
     CDS             complement(10906..12195)
                     /locus_tag="LK694_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBK5052659.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12345..13391
                     /locus_tag="LK694_001811"
     CDS             12345..13391
                     /locus_tag="LK694_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5052660.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13393..15546
                     /locus_tag="LK694_001812"
     CDS             13393..15546
                     /locus_tag="LK694_001812"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HBK5052661.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(16019..17470)
                     /locus_tag="LK694_001813"
     CDS             complement(16019..17470)
                     /locus_tag="LK694_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5052662.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(17467..19191)
                     /locus_tag="LK694_001814"
     CDS             complement(17467..19191)
                     /locus_tag="LK694_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK5052663.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(19184..19798)
                     /locus_tag="LK694_001815"
     CDS             complement(19184..19798)
                     /locus_tag="LK694_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBK5052664.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYSSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
BASE COUNT         5704 a         4127 c         3593 g         6614 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt taatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa agattatact
    12241 tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact ttttcatcca
    12301 ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgact caaccattgt
    12361 accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta cctgtagaca
    12421 gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga atcacttcca
    12481 aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc gggaaaggca
    12541 gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa acacgacgga
    12601 ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc aagggattga
    12661 accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac tttctacaaa
    12721 ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat gctcttgata
    12781 cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct gtcgtcatat
    12841 tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat ttccaaggcg
    12901 gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct tatttgtttg
    12961 gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt gaagcattgg
    13021 acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca ttagatgaat
    13081 tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac gatgttgtcg
    13141 ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa gatttttcaa
    13201 ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg acaaccatcg
    13261 cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact tggttagaga
    13321 caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga caatcaacaa
    13381 aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc gaatcagcca
    13441 aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat gaattatttt
    13501 gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga ccgcgttttt
    13561 caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag tcatatgctg
    13621 tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca aagttcttac
    13681 cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt acgttatggc
    13741 atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga ttacagcaaa
    13801 aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt tatagatcct
    13861 tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga tttttctttt
    13921 tattttatat tgagatttga atatccttta acaattgatg agttagctgt ttccaacaaa
    13981 aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat ccgatttggg
    14041 acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca aaaccaaagc
    14101 aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga tcgtatacag
    14161 ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata tcgggtgttt
    14221 ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca ctatgatacg
    14281 actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg ggatacgtat
    14341 aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga aatgctggaa
    14401 ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc tcctgatgaa
    14461 cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc tgtaaaagat
    14521 atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc tacggttcaa
    14581 agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata tggctatgtt
    14641 cctaatcact accacgaatc agtcaatcac acattagact atgcttatag cgatttttgt
    14701 attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata tcagcagcaa
    14761 tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc aaaagataaa
    14821 gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta tgcagaagga
    14881 agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat cggaaaatat
    14941 ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc cccaacgttt
    15001 gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc gatagaattt
    15061 ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt cagttatata
    15121 ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac cttcgacgat
    15181 tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg gtatatcttc
    15241 aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat cggtatgcca
    15301 ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat tgaagcaacg
    15361 cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacaa agaatatacg
    15421 aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta ccaattaggg
    15481 atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt atcaaactat
    15541 aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa
    15601 aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa gcttttgaac
    15661 accgtttatt gagaggttgt gaaatcagcg ttctgacctg agtattagag agacaaatgg
    15721 caaaatagtt cctcatattt tgccattttt tgagctaata ccgagggtca gctgattgaa
    15781 caccgtttag tggagaggtc gtgaaaaagc tgtttagttt cgagtattaa gaaaaatttt
    15841 gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgagaa actggctttt
    15901 gaactccgtt tatcgagagg tcatgaaaaa gctgccttgc ctcaagtaat aagaacagcc
    15961 caaaattaga tttctagaca attttttata agttatttca ttacattggc tttttctttt
    16021 atgattctcc actgttttct cggtactctt taggggatct gccgacgatt ttcttgaata
    16081 atttagagaa atacgtggtg ttgttgtagc caatctcact tgctatctca ttgatgtttt
    16141 gattactgtg caaaagcagt tgtttcgctt tttccatacg taaatggttc aaataagctg
    16201 aaaaactttt tttcgtttct tttttgaaca gctgtcctag atacatgaca tttaaatgca
    16261 ggttttctga cacttctttg agtgtaagtg gttcttgata atgctggtgc aggattacga
    16321 ttactttttc cacattgtca ctgtactttt tctgtctctg atgctttttg atgaatgaca
    16381 gaagtagctg ctgaagttct tgcaccgttt ttgcctgatg gattttttcg attccttgta
    16441 aatattcctc atcttccaga tcaatcaatt cccgttgaat ctccataaac agtaaaaaac
    16501 tggaatatcg tatatcttcc ggcatcatgg ctgctaactg aaactgttca aaaaaatcag
    16561 tgatcatttt ttgagcgact tccaattgtt tattttgcag cacgcgtctg aaacttgaaa
    16621 aatcgatggt ttgatctttt gtttctgctt gtactgcgta aaaaacatgc tcgtctttat
    16681 ctccataaaa ttgatgcaac tgtagattgt ctttcgcttg ttggaatgtt tcagggattt
    16741 tgtccaaaga atctgtctct tccccgatac tgatcagcca ttcctgatca ggaaagtttt
    16801 cttgaacaaa acaacagaag ccatcaactt catctgcctc caacaacaac gtgatcaata
    16861 cacggtctcc gtaatttcgt tgatagtaaa agatttgttg ccgttcttgc aaccatctat
    16921 tgataagagt atcatgagtt cttgccagtt gaatcaatac tatcctatgc tgcttctcac
    16981 ttatttgtcc tattatttct tcttcactta tttcatccaa ttcttcattc aaccattgac
    17041 tgaataacag ttcttgatag atttcttgtt gatttttttg ttcattttgc tgatctaacc
    17101 gtgttttgat tttcttgagg ctttcaacca attcgaactt attcacgggt ttcatcaaat
    17161 aattcacggc gcctaattgc atacctcctt tcaaataatc gaattcttgg tatcctgata
    17221 aaatcataaa ttcaaaatta tgacgttctt tttgagccgc ttcaataaat tccagtccat
    17281 ttatttctgg cattgtcaca tccgttacta ctaaatcaat ctgtcgctgt tcaagtacag
    17341 ctagtccttc tttggcattc tctgctgttg ctactatttg aaagcctaaa ctttgccaat
    17401 caattatctt ttttaaccca ttcaaaatca tgtattcatc atctaccaat aacacttgat
    17461 acacgctcat ccctccttga atgtaataga aacggtcagt ccacctgtct cattattctc
    17521 aagcgacata taataactgc tgccgaagct ggcacgtaaa cgttcattca cgttttgcag
    17581 accgatagaa ccgtgaagtt ccactttagg atgagacagt ttttcttcaa tctgttttag
    17641 ccgtttttca gtcatccctt ttccattatc tttgatgatg atccttaccc gtttgccttc
    17701 ctgcaagact ttgacactta gcgcattatc gaatcgagta aaatcaatac cgtgtttaaa
    17761 ataattttca actaatggct ggatcacaaa tttaggtact tcgatctttt ccaaatcagg
    17821 atcgatcata aaatgatagg ctactcgatt aggataacgc atttgataca aatagacata
    17881 tttttcgcaa aagtccagtt cttcttttaa agtgatcgtt ttctcttgat tcgtattgtt
    17941 tcgaagcaaa gcggaaaacg catacacaac atctgctaat tcttcacttc cttcgctgat
    18001 tgcatacatt cggatatatt ctaatgtatt atataaaaag tgtggattga tctgtgcttg
    18061 aagcgcccgc atatgtgcgt cttgctgttt gatttctaat ttgtagatgt cttctacaaa
    18121 ctgattgatg ctatccaaca tttcgttgat accgattgaa agttccttca gttcatattc
    18181 cgtgtttgtt atgtcgatcc gcgtctctaa attccctgct gctgtttgat ccatggcctg
    18241 catgacgata gatacttgct gtgaatatcg tttgaatgtt ttataaagaa agataagcag
    18301 tatattgtct aaaataatga taccaatgat cagcggggca agattttttc ccgtttcgat
    18361 ttttacactt tttttatcta gtacagctaa tatacgataa tcatcagaca gttcttggtg
    18421 ttgaacaaaa ttgttttttt ccagatcgtc aatcggcaat tgtgaatttt tcttcagact
    18481 ctctgtgatg atcatttctt gtctttcaaa cgtttcttta gctattttat tatcatggaa
    18541 tgtgtatagt cgatttgtcg ttccagaaat catgtataaa gagagtccat tgaatgacgt
    18601 tagattttct aaaatcatat ctagttcttt cttttgaaaa ccaataaaaa tacttcctga
    18661 ctcagctcct ccttgtgtga taggcaactt gatataaaag gcgtctagta attttggtgt
    18721 cccctttttt atttccccac ctttgttttc tctagaagat tcataatact ccggtagttg
    18781 attcaacacg atatatatcg cacttagttc ttcataattt gcgtagagat ctttgatttg
    18841 atttgggaaa gaaactaatt catcggtact gttttgctgt tcgtatacat acatcagata
    18901 ctgatctatg ggttttgtta agtaggtcgt cacattttcg atcttttcgg aattccctac
    18961 aagctggttt ttgatgatcc tactcaaggt tgttttatca ttcagcattc tgcttgtgct
    19021 ttgaacggca tcacttgtca tgttttctgc ctgtttttga ttttgttccc aagtatggat
    19081 actaaaaata acggtagcag tcatcgttac accgatcatg ataaccgcgt atttccgcaa
    19141 cagacgattt agtaaggttc cttttgaaaa tagaatccga aacttattca taactttcca
    19201 actgtttatc taattcttca cgccaatgtg ttgtcatcgt accattccaa accgtcaaaa
    19261 gaccaatgac accaaacagg atcaatcctt tgaattgcca agtcacgcct aaaattatac
    19321 cgctaccaat aattattttt agaaaagacg aaaagctgga aaaaaccgaa ataaaactta
    19381 gctttagcag attaggaaca gagatcgtat attcactatc taaaatcata ctgtattgat
    19441 aagtagcaaa taggcagagc aaggcaaaaa gtaaaataaa atcaatagct aaaaaaatga
    19501 gccctttgat ttgaacagat gaatataaat tgtaactcaa taaagcagta ccagataaaa
    19561 ataaactaaa tagcagattg cctcttaaaa aatttctttt atatatattc cagcctcttt
    19621 ttattgtgat ttctttatac tcgaatccgt acaaataaaa tagttcattg actgttttcc
    19681 atgccgcacc taatccaaag atgatcccgc cggcaaatgt atatatccag aaatacaagg
    19741 ttagttttat aatgacccag atccggataa ataactcttc caaaaccttc cctaacatat
    19801 cttctcctcc aaacattaag tttatacttt ctcattatac aaaaaaaggt tgggcaatca
    19861 gcaccttgac ctgattatta gagaaacaaa cggaaaaata gttcctcata ttttgtcgtt
    19921 ttgtgagcga ataccgaggg ttagctgatt gaacaccgtt taccaagagt ttgtgaaata
    19981 gccgcttagt ttcgagtagt aagaaaaatt ttgtgaatat ggctttccac atttttac
//
LOCUS       DAFBQH010000048        19555 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.050, whole genome shotgun sequence.
ACCESSION   DAFBQH010000048 DAFBQH010000000
VERSION     DAFBQH010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.050"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            149..2380
                     /gene="pflB"
                     /locus_tag="LK694_001816"
     CDS             149..2380
                     /gene="pflB"
                     /locus_tag="LK694_001816"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBK5052665.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            2624..3385
                     /gene="pflA"
                     /locus_tag="LK694_001817"
     CDS             2624..3385
                     /gene="pflA"
                     /locus_tag="LK694_001817"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBK5052666.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            3470..4396
                     /locus_tag="LK694_001818"
     CDS             3470..4396
                     /locus_tag="LK694_001818"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBK5052667.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            4578..5471
                     /locus_tag="LK694_001819"
     CDS             4578..5471
                     /locus_tag="LK694_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBK5052668.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            5605..5730
                     /locus_tag="LK694_001820"
     CDS             5605..5730
                     /locus_tag="LK694_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5052669.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5864..6496)
                     /locus_tag="LK694_001821"
     CDS             complement(5864..6496)
                     /locus_tag="LK694_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052670.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(6644..7186)
                     /locus_tag="LK694_001822"
     CDS             complement(6644..7186)
                     /locus_tag="LK694_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBK5052671.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGVLIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            7321..7710
                     /locus_tag="LK694_001823"
     CDS             7321..7710
                     /locus_tag="LK694_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052672.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            7714..8478
                     /locus_tag="LK694_001824"
     CDS             7714..8478
                     /locus_tag="LK694_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBK5052673.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            8607..9554
                     /locus_tag="LK694_001825"
     CDS             8607..9554
                     /locus_tag="LK694_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5052674.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            9684..10424
                     /locus_tag="LK694_001826"
     CDS             9684..10424
                     /locus_tag="LK694_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052675.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10715..11383)
                     /locus_tag="LK694_001827"
     CDS             complement(10715..11383)
                     /locus_tag="LK694_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK5052676.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            11517..11804
                     /locus_tag="LK694_001828"
     CDS             11517..11804
                     /locus_tag="LK694_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052677.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            12220..13758
                     /locus_tag="LK694_001829"
     CDS             12220..13758
                     /locus_tag="LK694_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBK5052678.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            13755..14720
                     /locus_tag="LK694_001830"
     CDS             13755..14720
                     /locus_tag="LK694_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5052679.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            14707..16029
                     /locus_tag="LK694_001831"
     CDS             14707..16029
                     /locus_tag="LK694_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK5052680.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      16092..16196
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            16270..17013
                     /locus_tag="LK694_001832"
     CDS             16270..17013
                     /locus_tag="LK694_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5052681.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYIPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            17028..18020
                     /locus_tag="LK694_001833"
     CDS             17028..18020
                     /locus_tag="LK694_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK5052682.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            18032..18769
                     /locus_tag="LK694_001834"
     CDS             18032..18769
                     /locus_tag="LK694_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5052683.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            18759..19481
                     /locus_tag="LK694_001835"
     CDS             18759..19481
                     /locus_tag="LK694_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBK5052684.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT         6721 a         3300 c         3978 g         5556 t
ORIGIN      
        1 ggtatgtcaa cgttgattta aatatatgta aataaaacat aaaaattgtt aaataaaaaa
       61 caagaaaacc cttgcaaaga tagggtgctg tgttatacta atcacgtaaa aactgttaca
      121 taaaacagaa atttaggagg tccatacaat ggagcaatgg aatggattta aaggtaccaa
      181 atggaaagaa ggcgtagaca ctagagactt tattcaaaat aactacactg aatatcgcgg
      241 tgatgacagc tttttggaag gcccaacaga agcaacagaa aaattatggg atgaattgca
      301 aaagttaaat gatatccaat tcgagcgtaa cggtgtctat gatatggaca cagaaatcgt
      361 atcaactatt acttcacacg atgcaggcta tttggataaa gatttggaaa aaatcgtcgg
      421 tctacagaca gacaaacctt tgaaacaagc attcatgccg ttcggcggaa tcaatatggc
      481 taacaatgcg ctaaaaagca atggctatac accaaacgat gaattgacac atattttcac
      541 tgactggcgc aaaacacata atcaaggtgt atttgacgct tatactgctg aaatgcgtgc
      601 tgctcgtaaa aacaaaatca ttacagggct tccagacgct tacggtcgcg gacgtatcat
      661 cggtgactat cgtcgtgtgg cattgtatgg aatcgactat ttgatggaac aaaagaaaaa
      721 agatcatgat atgactggtt atcatacaat gagtgaagaa gtgattcgtt tacgtgaaga
      781 aatcactgaa caatatcgtg cattagcgca aatgaaagaa atggctgcga aatatggcta
      841 tgatatttct aaaccagctg caaatgcaaa agaagctgtc caatggctat acttcggata
      901 tttagcagca atcaaatcac aaaatggtgc tgcgatgtca atcggacgta tctcagcatt
      961 ccttgatatc tatatccaac gtgatttgga taatggagta atcacagaaa aagaagcaca
     1021 agaattgatc gaccacttag taatgaaatt acgtatggtc aaattcgcgc gtacacctga
     1081 atataaccaa ttattctcag gattccctat ctgggctact ttatccattg caggtatggg
     1141 attagatggt cgttcattag ttacaaagaa tgatttccgt ctgcttcata cattaacaaa
     1201 tatgggacct tcaccagaac caaacttgac tgttttgtat tctgaacacc ttccagaagg
     1261 attcagaaca tatgctagca gaatcgctat cgaaagttct tcgattcaat tcgaaaatga
     1321 taatcagctt cgtgaacaat ggggttctga tgattgtgcg attgcttgct gtgtatcagg
     1381 aacagttgtc ggaaaagaca tgcaattctt tggcgctcgt gctaacttgg ctaaaacact
     1441 attatatgcc atcaatggcg gagtcgatga agtgactggc gcccaagttg gaccagaata
     1501 tcgtccaatc acatcagaaa tactagactt tgaagaagtg aaacataact tcatgaatat
     1561 gatggattgg ttagcagaac tatatgtcaa tacgttgaac atcattcatt atatgcatga
     1621 taaatattct tatgaagcag ctcagctggc tttgatggat tctgacttgc aacgcacatt
     1681 tgctacaggt attgctggga tctctcatgc ggcagacagc ttgtcagcga tcaaatatgc
     1741 aaaagtaaaa gcaatccgta atgaaaaagg aatagcagtt gattttgaag tggatggcga
     1801 cttccctaaa tacggaaatg atgatgaccg tgcagatgaa ttagtttcat ggatcttgga
     1861 atacttcatg ggacaaatca aacgtcataa aacttaccgc aactcaaaac caacaacttc
     1921 attattgacg attacttcaa atgttgttta cggtaaaaat acaggaaata ctcctgatgg
     1981 ccgtgaagct ggaaaaccac ttgcgccagg agcaaatcca agttacaatg cggaacaaag
     2041 tgggttatta gctagcctga actcaacagc taaaatgaat tacggctgtg cattggatgg
     2101 aatttcaaat acccaaacaa tcaatccttc aggtcttgga aaagaacaag atacaagaat
     2161 cgaaaacttg cgtaatgtct tagatggata cttctcaaaa ggagcttatc atttgaatgt
     2221 caatgtattc agtaatgatt tattgcgtga tgcaatggaa aatccaatga aatatccgaa
     2281 cttgacgatc cgggtatctg gttatgcagt taaattccgt gacttgacaa gagaacaaca
     2341 attagatgtc ttgatgagaa cttcacacga cagaatgtaa aaagttcgtg aaaaaagttt
     2401 ttcgacttag cgtcgaaagg acgttgaata gccatcgcat caaaatcgaa taatcagtat
     2461 gcttatttga aagaaacgat ttacaggggc aggacttgta tgttccgtcc ctgtaaattt
     2521 tacacataac gaagcggcaa ttgcgaaaaa ttttgttcag aaagcttcgc ttcgacagct
     2581 aacttgtact taaaatgaaa tcaaagaaaa gatgtgaact taaatggaag aaaaaacaat
     2641 cggctatgtt cattctatcg aaactttcgg ttcagtagac ggacctggac tgcgctttgt
     2701 cgtgtttatg caaggatgcc gcatgcgttg ccaattttgt cacaatccag atacttggaa
     2761 tattggcgga gggaaagaat acacagcaga tgaactttta gataaagcag aacgcttcag
     2821 accttactgg ggagataaag gtggtattac tgttagtggt ggagaacctc tattgcagat
     2881 cgattttttg atcgaactat ttaaaaaagc aaaagagcgg aagatgcata ctacattgga
     2941 tacatgcgga aaacctttta cgtatgaaga acctttcttt tcacgttttc aagaattgat
     3001 gaagtataca gatttactgt tatttgacat caaacacatc gataatgaag agcataaaaa
     3061 actaacgcat tgggacaatg aaaatatttt agaaatggct caatatttgt caaaaatcaa
     3121 caaaccggtt tggattcgcc atgtactagt tcctgagcga agtgattatg atgaatattt
     3181 gatccgacta gacaacttta ttcaaacctt gtcaaatgta gacagagtag agattcttcc
     3241 ttatcatacg atgggaaaat acaagtggga aacacttggt ttgaaatatc cattagaagg
     3301 gatcgaacca cctacaaaag aacgtgtcga aaatgctaaa cgattgcttc atacagaaga
     3361 gtatacaaaa tatttaacgc gataggtcgc atcggaaaga agataagtat tgcttctttc
     3421 cgtttttttg ctattattaa ataggagaaa tcacaaagga gcgaatgaca tgtcaaaaat
     3481 tttagttttt ggtcaccaaa atccagatac agatgcaatc ggggcagcca ttgcgttttc
     3541 acacttgcaa aatgaattag gtaaagaaac agaagcagtt gctctaggta cacctaatga
     3601 agaaacacaa tacgcattag accactttca gctatctgca ccacgagtag tagagtcagc
     3661 taaagcagaa aatgcagatg tcatgcttgt ggatcataat gaattccaac aaagtatttc
     3721 agatatcagc gaagtaaata ttttagctgt agtagaccac catcgtattg caaactttga
     3781 aacagctaat ccattgtact atcgtgcaga accagttgga tgtacaagta cgattatttt
     3841 aaaactatat aaagaaaacc aagtaacaat tcctaaagaa atcgcaggaa tcatgctctc
     3901 agctatcatt tcagatacat tgttgttcaa atcaccaaca tgtacgcctg aagatgaagc
     3961 agctgcaaaa gaacttgcag caatcgctga agtcgatcta aacacttatg gacttgaaat
     4021 gttgaaagca ggaacaaatc taagtgataa atcagctgcg gttcttcttg atttggatgc
     4081 aaaaagtttc ccaatgggca gttccaacgt tcgtatcgct caagtcaata cagtggacct
     4141 taatgaagtg atggatcgac aagcagagct agaagcagca atggaagatg aaatgaaagc
     4201 aaatggctac gatctgttcg tattagttgt cacaaacatc ttagacagtg attcagaatt
     4261 acttgttgct ggtaaaggaa aagaaaaagt agaagaagct ttccaaacaa ctttgaccaa
     4321 caaccgtgct ttgcttaaag gtgtcgtttc acgtaaaaaa caagtcgtac ctcaattgac
     4381 ggctgtattt agctaatcaa atggtagtat gaagcattca aaatgaatgg ataagcgatt
     4441 gatataaaaa gaaacataga acatactaaa ggccgaagaa atggaggaga ataacagtca
     4501 attatgactc ttgttcttcc tcctttttac gtaaaagcct tttaagtaaa ataaaacgga
     4561 tagaaaagag aacatatatg aactattatt caaaaaataa ggaaaacttt tatcaaaaac
     4621 tgaccgatga tccgcttttt tcttcattga ccgattatct atatgaacat cgagaacaag
     4681 aaacgattct aagagaattg aaaaaagaat tttcgcaaaa taaattttcc cattttcttg
     4741 atttattgat cgatgcagga ttaatcaaga gggaagaacg aagatatcat ttgaactttc
     4801 caatatttga tcctaaagat tacttgcaac aagccacttc ggctgctgaa accatagctg
     4861 aacaattgaa gagactctct gttgatgaac aaaaattagc gatgggagaa gttatttggg
     4921 cgtattgttt cgaagatgaa cggaaagaag cgtattttta tggagtaaga aacagccgag
     4981 agacagaatt gctgaggaca actgcaggaa atgagaagta ccgttttatc acactttcct
     5041 ctatagaaca cttcccgctt actttggcta attacttttt tgtacagaag aatcagcttc
     5101 ctgtaacgaa ggcattcaaa gaattagccg aacttattgg agacgtaaat gaatcgtact
     5161 tttttgatca aatagaagtt atcgttgaca ggatacgtaa gaataaatat aaaaatcgcc
     5221 ggcctagtat tttccatcag tctctacttg tgacgaatac tataaaggaa gaagaatcat
     5281 tcacattaga attacctatt gtcgaaaaaa acaattttga gattgaatta ccaacattgg
     5341 atccttcatt gacaatggag gaaatggcct ttcttaaacg acaaatcttt tctgagctga
     5401 gcaagaaatt tataccgcat gcattttctt atatcaaaga atatggaaca gttttagtga
     5461 gtaaaaccta agagagcagt aagcaaaaat tatactgact cttgcaggag gagcctttct
     5521 cttcctttat ggttaaaaga attatttgag tattttgcct atctaccacc tattagattc
     5581 actacatggt ctggaaacaa aggaatgcaa aaaaatggtg agaaatgtgg aatgacaaag
     5641 gaagtagtga taagaaaagt tagattttta aataatcaat attatgattc agtcaagtat
     5701 ggcattttat gggaagaact agctgattag attaggaagt ccatttgata atccagaatc
     5761 actaagaaac gaatggcgag tcattaataa tttatccaat cttgggttat tcttttagat
     5821 tgaagtgttt aaaactaaaa aagcatttat gaagtcgtaa tagtcaggct tcaaaatgct
     5881 ttttattttg tttgtttttt ctttgccaca taaatacacc tagactgacg atgaagagca
     5941 tatccaacag atttcctgcg acttggatcg tcgaagggtt ggtcagtggc attaaagcaa
     6001 ggacaaaaaa ggaaaacaag ctgtaaataa aacaggtgac aggaactaat cgaaagaaga
     6061 gcggacattt caattggcta aaaatgagtg aaaaaataaa ataagtaaaa atggataaaa
     6121 tcacgactaa accattcatg ataaagaaga aaagcgtatt gtgattaaac gaataaaaaa
     6181 gaattgctaa aaaagaaaac gttttaaatg caatcaatac gcttgaagct tcgtttaacg
     6241 taccaggatg tcgatgaaaa aaataggcac cgaaataaac agaaaacagt aagccgcaaa
     6301 gagaagacaa aatcaaaaaa aacggatttt cttcggcttc tgtgatatca gaatgatcca
     6361 aaagcaacca gcctaatgca ctagaaggcg catgtgaggt cttaaggtat gaataacata
     6421 gaaaaaataa gatcgtcaaa gttgcaaaaa aagagcctaa atgtaaaaaa ttagtggttc
     6481 tacgttgaat ttccataata tctccaaatt aaattgataa gtatataaag tataataaca
     6541 aattgatgag tatatgtcga tgttttttaa tcaaaataat aaataaaaac tgtctaacat
     6601 ttaaaattta atttagatat tagacagttt ttaagtttgt gagttatttt ccttgtcgtt
     6661 ttctcacttt tttagtccag aaaccacctg aaatatattt tggctcataa gaaatgataa
     6721 aagcacgctc ttcgatttct ttgatcaggc gatacagttc acgttcgctt tttctaggag
     6781 aaagtatttc gagaactagc cgctcacctt ccagccccat tgcagaagtc tgtgtcacac
     6841 cataaccgtg ttctctcagt atacccggca aacttttatc ttctgcgcta tttgctggca
     6901 aaatcgttgt gaccatgatg tatcctaatg ccaatttgtc ttcaatcaaa actccgacac
     6961 tgatacctac tgcgtaacct aacgcataga caaataaatt cagtggatta tctaaacgat
     7021 ctaaaacaag ggataagccg acgatataaa tcgtgatctc gaccatactg acaagtggag
     7081 caatcagtcg atagcctttc attgtcaaca tgaaacgtaa cgtgttcaga gtgatataag
     7141 caaaattaat gaggaagatc atccccaaca tttttggatc aatcatttgt aaaactcctt
     7201 tcatttatat aacaacaata tcaaaaaaaa tcgtaagtga agaaaaattg aaactttata
     7261 catacaaaaa aatgataaaa tgattataat agaattataa agataggtta ggtgattaaa
     7321 atgaaaacgg tcaatcgtgt gttttgtgcc agccacgtgc taagtctcct tctcagtata
     7381 tacttaggtg tctattcgat tcaacagcca attgaggtag agaacattgt ttttcagatg
     7441 tcgctgacag atggtttaat tttaagcgtt tttttctttt tgatactgtt tattggtaat
     7501 attttcggta gcgttgccag ttttttgaat ttttctattt atccactcct ttcgctagca
     7561 ttaggagtgg tgggactttg ttctctgtgc ttatttcccg agccattcag tcctgcattt
     7621 atattatttg gtacactgaa cctgttccag acaactgttg gagcatggtt gctatggcga
     7681 tcaggaaatt taatgaaaat aggtgagtaa caattgaatc caattatatt gaaacgttct
     7741 gtccatcata ttccagtgtt agaagttgtt ctgaaagaat taagagagga acgtattccc
     7801 gttgtcatct attatcacgg atggcagaca agtaaggagt tagttttgac gcaaggaaga
     7861 aaaattgcgc agcagggcat tcgtgtgatc ctgccagatg cgatgaatca tggggagcgc
     7921 aaacagccgg tttcttctat tccatcctat acattttggg atagtatcta cggaaatctg
     7981 tttgaatttg acacacttat caagcattta gaaaaaagag gtcttctaca tgatcagctt
     8041 gcagttggcg gggtttcgat ggggggaatg acaacatgcg ccttgttggc taaacatcct
     8101 catatagtag cgggcgtgtg tttgatgggt tcccctgcac ctttggaata tggaaaagaa
     8161 atcgtaaggc gcgcatcgga atataattat gtcgtaccaa aagactattt tgatttgatt
     8221 tcatgggtta agagctatga tttgtcattg cagccggaaa agttagctgg acgtcctctg
     8281 ttcttttggc atggacaagc tgacgaaaaa atcccttata tacaaacgga acgatttgta
     8341 gaaaaaaatc ggcataagtc ttacggaaaa gaaattgttt ttcgttcctc aaaaacacag
     8401 cgacatatgg tacaaatacc actaatggaa gaagcttcca cttttttagc aaaaaatttg
     8461 ataaaaaagt ataaataatt gaaacggaaa atcactcatg acgatagagg attttccgtt
     8521 ttttattttg ggctataata aactctgtta ggaaaatttg gtataattga tagtaagaat
     8581 gacaacatgt tagaaaggaa gagagcatgc ccactttagc agatgttgca aaacgtgcca
     8641 atgtttcaaa aatgacggtc tctcgagtca tcaatcatcc ggaacaggtg actgacgaat
     8701 tgaaagagtt agttttctca gcaatggctg aactggatta ccgtccaaat attgctgcaa
     8761 aagcacttgt cacgaatcgg tcgcagatca tcaaattatt tatactggaa gaaatcgaca
     8821 caactgagcc ctattacatg aatttgctga tgggaatcgc taaacggatc ggtaaagcgc
     8881 attattctct ccaattagtt acaaatgatg ccttcgacgt cggttcttgt gatggataca
     8941 tcatcacagg tgtcagaaaa aaagacttcg attggatcga ccgtttggaa aaaccagttg
     9001 tcttgtttgg ggaaaattct gaaggatatg attatgtaga cagcgataat gaatatggta
     9061 cagcaaaagc aacgcaatat gccttagaaa gagattatca aacgattatt tatatcggta
     9121 tcgaagtgga cgagcctttt gaaatttcaa gggaaactgg ttatttatct gtgatgaagg
     9181 aagttgatct tcctagtaaa atcttgcggt ttccaaatca ttcgacagaa gtagaaagat
     9241 atatgacaga aaactgggca gattttacag ataaaacttg ttttgtatgt agttcagatc
     9301 ggctagcact aggagcagta agagggatta taaatgctgg aggacacttg cccgaagaat
     9361 ttggtgtgat tggatttgat ggggtatttt tagaccaaat cagttcacct aaattgacaa
     9421 cgatcaagca agatattata cgtatgggtg aagtatgtgg agaaatgttg ttaaaaaaaa
     9481 tagaagaaaa aggggcgagt caaggttatc gtcgtttttt tcctgaacta gttatcagag
     9541 agacaacgaa ttaagtctta ttactatata tatatttatg aaattcatcg ctattttctt
     9601 aaaataatca gcgcattgct actgaaaaca atgaatctgt gctattattg agaaaagttc
     9661 catttttgag aaggatggtt tgaatgaaag aaatcagacg aaaacatttc cgttttcctg
     9721 cgtacgatga tgagttaggc gtaaaattaa ccaacgacaa gaagcgagta ttatttgacg
     9781 gacaagaaga tcttttgact gaacagaatc agtccccgtt tgaacagatg aaagaatttc
     9841 atttcacgaa cacgaacacc gagaaaatcc gtcgcaagcg tcgtgcaaca aatggattga
     9901 agtcagctcc gcgtaaggaa aatttggaaa agcataaagg aaaattaccg gattataccg
     9961 gccattacca atcagaaatc acatcgacag gacgaaaaaa actttttggt gattcctctc
    10021 gttctgtcag acaaagtgaa acaaggacag agtccaagca tactacacga aacgcatctg
    10081 ttgacacgaa ggtgcgggag cgttcttatt ttgttcctaa atatgtaccc gcatcgatta
    10141 tcccagaccc tagagaaagc aaaatttctg aggatcattt aatcgaatcg atgaaaaaaa
    10201 cggatgactc ttatctgttg ttcgatacag aaccgacgcc gtatcaagaa aaaagaaatg
    10261 gtgagccgtc tgtacgaaca ttcaatcgaa caaaaacgtt agaggaaaca aagcagtcag
    10321 aagaaaaaaa atcgaaaaaa agccatggta tcttagaacg ctcattacaa ggattgatcg
    10381 atgatcaagg aaactctttg ggagaaaata gctattttaa ataagtgata tacaaattta
    10441 tattcggcta cagttggaaa ggaaacgctg cggcgtttcc ttattttata ctctatccga
    10501 aagtttatat cgtatcctca aatttttttg agtttaagcg aattttttct cccaataagt
    10561 aagaaaataa tggcaaaata aaaaaacaaa ctattttcgc agatcgtttg caaaaatagt
    10621 ttattttttt ataaagaaga ttttactgaa taatatcatt ttagtttaca taatgatatt
    10681 tctgtaaatt aaataaagta gtgaaaaata agaatcagtg ctctaactgt tcaagaggat
    10741 caagtggttc aaatgtttct gtagtctgtt ttgcttccaa ttggggcaaa acagatccaa
    10801 tttccagata cttcacttta ttcaatgtat aagatagaat agaacagcta agtaatgtat
    10861 acagaaaatg tgtgagatcc aaataaagca aggagacaac taaaatacca aaagtaatca
    10921 agaaaagact tttgccaatc ttaatatgaa aatatttttc aatggcttga taaatgatat
    10981 ctgttccgcc tgtgcttccg ccgaatcgaa agacgagacc gcaaccaatg ccagaaagaa
    11041 tacctgtcat caaactattg atcagtagac tatctacagc aaaatgtata tgaaggctct
    11101 cccataaatg taaaaagaaa actagcgcaa atgttccgac gatcgtccgg agaaaagcgt
    11161 gttttccaaa aagaaacaat gagataagca ataaaggtaa attaaaaagc agtgaagtga
    11221 cagaaggagc gaatccaaaa agattatgca aaagcaaagc aataccagta accccaccat
    11281 ctgccagctt agatggaata ttgattcctg tcacagctaa tccgtagatt cctgcgccta
    11341 aaatgattcc gcacaattct gtaaataaat ttaatttttt catttctagc ctcttttctt
    11401 ttgaaaattt tcagcaactt ctttattata ctgacttata ctcttacttc aagaggaaaa
    11461 tgaaaaaaaa ttatttttgg ctgtttcttt ttatttatca aataaggagt ggaaaaatgc
    11521 gagcagcaga cttattgaca attcttacaa aatcacccaa aaaaacagag tatcaaggag
    11581 tttttttggc tcaagaacaa aaactggtgc ctgtgacaat ggtccagata aataaagcaa
    11641 atcaatttat ctttttattt gaacacggca aagcaccact tgctatgaaa gacatctttc
    11701 tttttttgat gaagaaccgt gagaaagctg tttattatca gtcaaaagaa gaactcattc
    11761 ctctttatgg agtgaaagag atagaaaata aagtggtgat ataagtaaaa ataagaaaac
    11821 aagacgttta tgatgtcaaa acaaccattt tttacatttt tggcaaaaaa gtgtcatgat
    11881 acagtataat attcgttgaa caggccaata aaggtgttct cccccacgag atttctactt
    11941 tattggctgt ttctttttat taaaataaaa gttgtgtgtg taaaaaagat atagctgatt
    12001 ccaagtgaca atttcgtata gatttttatg gtattcgtag tgaaaaaacg agaaccaaaa
    12061 agttgatccg actttgttgc ttggattctt tttttgtcag catatttaga ggtatttttt
    12121 tctttttttt gttcgtgctg taaggttaag gcaagacaag ttagttggga aaaaggaaag
    12181 aaatgtttat acttaaagaa taacagaaag gaaggtcgca tgaataattt tatacaaaca
    12241 ttaagtgaaa gaaaaggcga gttgctggaa gctgcatttc aacatctgtc catttctctc
    12301 atttctttgc tgattgcagc actgattgct attcctttag cgatttgggc agccaatcat
    12361 aaaaaaatgg cagaagtctt attgcagatc acgggtgtgc tccaaacgat tccttcactt
    12421 gccttattag gtttacttat cccatttgta gggatcggaa ctgttccagc attgattgca
    12481 cttgttattt acgctttgct tccgattttc caaaacacgt atattggtct agctgaaatt
    12541 gacccgtcta ttgaagaagc agcggtagca tttggtatgt ccagaatgag acgcttgctg
    12601 aaagtagagc ttcccatcgc attacctgtc attatctcag gtatccgaac tgccttagtt
    12661 ttaatcatcg gtacggctac tttggcagca ttgatcggag caggtggatt agggacattt
    12721 attttgttag gtattgaccg aaatacacct gttttgacat tgattggtgc gatcagttct
    12781 gccttgcttg caattatatt cagcggactg attcgctggt tgcagcataa gaaacctcga
    12841 tattccatca ttactttagc agcagtggtg tgtttatttg gcggcgtttc tttatatcag
    12901 tctgccttgt ttaaaaatga aaccatcacg attgctggaa aattaggagc ggaacctgaa
    12961 atcctgatcc aaatgtataa agaattgatt gaagacaaca caaaaacaga agttgaatta
    13021 aaaccgaatt ttggcaagac gagttttttg ttcaatgcac tggatcaagg tcaaatcgat
    13081 atctatcctg agttcacagg tactgtccta gaaagcttgg tgaaggtacc tgaagcaatg
    13141 aaaagtgctg atttgacaaa agaaaaaaca tatgacgaag cagaagaatt attgaataag
    13201 caattcgata tgacattatt aaaaccaatg gcttaccaaa atacgtatgc cttggcagtc
    13261 aagagatctt ttgcgaacga acatcaactt aaaaagattt ccgatttggc agctatctct
    13321 gatcagatca aagcgggatt cacactggaa tttattgatc gttcagatgg atataaagga
    13381 atccaagaag catatggctt gaatttttca agtgtacaaa gcatggagcc aagtttgcgt
    13441 taccaagcaa tcaataatgg agatgtcaat attgtggatg cctattccac agatagccag
    13501 ctcaaggagt acgatctagt gactctagaa gatgatcgag ggttattccc agcttatcaa
    13561 ggcgcaccgc tgatgaaaaa agattttgct aaagaacatc ctgaaatcgt taaagcactg
    13621 aataaattaa gcggaaaaat aacagaggat caaatgattg atatgaatta ccaagtcaat
    13681 gtcgaaaaga aaaagccgac agaagtagca cgaatcttcc tgaaaaatga aggattagta
    13741 aaggaggaag atcagtgact aaattaatcg agttcaaaca tgtgcaaaaa aagtatgatg
    13801 gaaagtatgt catcgatgac ctaaatctgg caatcaaaaa aggagaaatc tttgttttgg
    13861 ttggtccttc cggtagtgga aaaacaacta ctttgaaaat gatcaatgga ttatctaaac
    13921 catcagcagg agacatctac ttcaaaggaa aatcgttaaa tgaatataat ctgcagaaaa
    13981 tgcggtggaa tatgggatat gtgctgcagc aaatcgcttt gttcccgacc atgacagtca
    14041 agcaaaatat tgaagtcatc cctgaaatgt taggttggga aaagcagaaa agagcagacc
    14101 gtgtagatga acttcttcaa aaagttggct tatctcctga tatttatcga gatcgtatgc
    14161 ctagagagtt gtctggagga gaacagcaac ggatcgggat catccgagct attgctgcaa
    14221 gcccagatgt gattttgatg gacgagccat ttagcgcatt agatccgatt tcaagaaact
    14281 cattacaaga attggttttg tccttacacg aagaattggg cacgaccatc gttttcgtta
    14341 cgcacaatat ggaagaagca atcaagctag gtgatcgtat tgcatttatg aaggatggag
    14401 aaattatcca atgtgacaca ccagagcagt tattgatgaa tcctaaaaac gactacgttc
    14461 ggcatttctt tgatgaaccg aaacagacga aagagtggcg agtagaagat ttagtcgtca
    14521 atggatattt cttaaatgaa ataccagaaa atgctcgtca acaagtttgc tttgatacgc
    14581 ctatgaaaga agtatattcg cttctttcgg tgtatccaag cattgtgata gtagaaaagc
    14641 aaagaagtat tgggtcgctg acaagtaaag aaatctttgc ttttttaagt cgagaggagg
    14701 aaggaaatga aaacatatga tgcaatcatc atcggttcag gtgtgagcgg cttaagtgct
    14761 gcttatggat tgaaagaagc aggtaaaaca gtactagtgg tcgaagaaga tctatggggc
    14821 gggacttgtc ctaaccgtgg atgtgatcct aaaaaagtct tgttaagtgc agtagaggca
    14881 cgtaatcggg taaaacaatt gagtggaaaa ggttttaacg agatcccaac tgccaactgg
    14941 gaagaacttc aaaaattcaa acgaacattt acggatccag tcccagaaag ccgaaaaaaa
    15001 caattagcag aagctgaaat cgatcatctt tcaggaaccg ctcgtttttt ggatgattca
    15061 tctattgaag tcaacgaaga agtcttccat gcggattact tagttttggc aaccgggcaa
    15121 agacctacga tcttaccagt ggagggaaag gaatacttga aaactagtgc agacttttta
    15181 tcattgcctg tactgcctaa agaaatcatt tttatcggtg gtggttatat tgcttttgag
    15241 ctagctacga ttgccaatgc tgcagggagc aaagtaacca ttgtccatca taatcaacgg
    15301 ccattaaaag aatttgaggc gtctttggta gaggaagccg tccatcaaat ggaagcatct
    15361 ggcattcagt ttgcttttgg tgttgaaacc caaaagatca tttcagaagg tactcgctat
    15421 cgtttagtcg gaaaagagac ggaattagta gcagatatga ttttttgtgc gactgggaga
    15481 cagccgaata cagaatcttt agcacttgag caagcgaata tcgtctttga taaacacgga
    15541 attgcagtca atgattatct gcaaacgagc aatccaaaaa tttttgcgtg tggagatatt
    15601 gtttcaagga aaaccccaaa attgacacca gtcgccactt ttgaaggaaa ttatgtagct
    15661 aagcggataa cggatgcaac aagcgaacct atcaaatatc cgattatacc tacgatcgtc
    15721 tacgccagtc ctaaacttgc tgaagtagga gttacaaaaa gtcacgcctc ttcgtcagat
    15781 caagtggtag agatggatct taccagttgg ttcacctatc atcgtgtcaa tgaaccagtt
    15841 gctaaggctg aattgacctt tgaccagcaa aattatttga ttggtgctgc agtgattagc
    15901 gagcaagcag acgaattgat cgatgatttg acactcgtca tcaatcaaaa attgactaaa
    15961 aaagagttgg atagctatat catggggtat ccgacattgg caagtgatct ctcctattta
    16021 ttgaaataat cagcatgata actaaagcgc ttgttgcttt ttccttcttg tgctacactt
    16081 taaaaaatga atacctgttt ctggggatgt cgtagacgac atgagagtaa gttgtgaact
    16141 tagacccatt gaacctgtca gttaatactg gtgaagggag aaacatagca gacgcttgaa
    16201 gcgggttact atgtcgtgcc catttcaagc gttttttgtt attcatatta gaagaaagga
    16261 ggtaacaaaa tgaagaaaaa tctttatcca gcagtcagta tggcattcat tttaattatt
    16321 tggcagattg ttgtcatagt ttggaaaata cctgccttta ttttacccgg acctgctgct
    16381 gtattttctg cattagcggc agatgcacaa gtattacttt cccattctgt cacgacatta
    16441 aatgaagcag tgcttggttt ggtaatagct agcgttcttt cttttattac tgccttggca
    16501 atggattatt ggaagttttt agaaatgtct ttttatccgt tactagtgat ttcacaaatg
    16561 ttgccaatca tggtgttagg gccattactt accctatggt ttggtttcgg tatgcttcca
    16621 aaagtgatcc ttgttgtctt gataagctat attccgatcg tagtttcttt ttcggataca
    16681 ttgaggaaag tatcaaaaga gcaaatcatc tttttgaaaa cgatgggggc agatacaggg
    16741 aagatttatc gaatatataa aataccagcg ggtttatccg gcttcttttc cggtttaaaa
    16801 gtggctgcta cttattgtgt cagcggagca gtcgttggag aatggctaag cgctcaagca
    16861 ggcttagggt attacatgat acgcgtaaaa aacagctacc agatcgataa agtttttgct
    16921 gcgatcattt gtgtcattct attgagttta ttgctgaatg gcgcttgttc attattgaaa
    16981 aaagggtatt atcgtatgct ttatattttt taagagaagg ggatcaaatg aaaaaattat
    17041 ggcttttatt accgctactg ttgcttttat ccgcttgtgg cactgcaaaa gaaacaagta
    17101 gcaaacaaga aataaaggat ttgaaaaaag tgacacttgt tttggattat gtaccgaata
    17161 ctaatcatac tggcatctac ctagcaaaag aaaaaggcta ttataaagag gctggtttaa
    17221 atgtccaaat catcgaacca ggagataatt cgacaagtat tggtctagta ggtgcggaca
    17281 aagcacaatt tggtgtaagt tatcaagaag atgtcactta tgctcacgca gatggacaga
    17341 atataccagt caaagcaatt gctacagtga tcaaacataa tacttctgga ttcgccaccc
    17401 tatcagacag taacatccat tcaccaaaag attttgaagg aaaaacatat gcaggttggc
    17461 aaagtccaag tgaagaagct gtcttaaaag ctgtgatgga aaaagacggc ggggactttt
    17521 caaaactgac aatggtagga agcaatggag aagggccaga aagcttagga aaatcctcag
    17581 atatccaatg gtactttgaa ggttgggata tgatcaaagc aaaagaagcg ggtattgaac
    17641 taaactatat cccattgaaa gaattagatg aacggttaga ttactatact ccggtaatta
    17701 ttacgaatga tcaactgatc aaaagtgatc cagaattggt acagtctttt atggatgcca
    17761 cgaaaaaagg ctatcaggaa gctattaagg atccaaatga cagtgctaaa ctgttgcaaa
    17821 aatatgcaaa agaaaatgat cggacgtttt tagaagaatc acaagcattc ttgtcaaaaa
    17881 attatacgga tgatcctgag aattggggat tgatggaaga aaaagtttgg cgcaattata
    17941 cagcttttat gcaggaaaac ggattgatca agcaagatgt accttcgcaa gaactattca
    18001 ctaatcagtt tataaaatag gagtgataga tatgtcaatc caaatcagcc acttgaatgt
    18061 agtcattgaa aacctgaaaa tattagaaga catccatttc actatcccta aagatacctt
    18121 tacatcgatt gtcgcgccaa gcggagccgg taagtcaaca ttgctgaaaa ctttaacagg
    18181 agtccagcct atgacttctg gaacgatcaa agtagacgat caaaaagtaa ctggtctcca
    18241 tacagcattt agttatatgc cgcaagaaga tatgttactt ccttggctga gcgtctatca
    18301 aaacgttacg ttatatcaaa aaatcaatca tctaacaatc aatgaagaac aagtaaggga
    18361 ttatctggat atctttgggt taaacggcta tgaacatttt ttgccggaac aattatctgg
    18421 cgggatgaag cagcgaacgg ccttgttacg aacaatcatg aatccttctt cttacttgct
    18481 tcttgatgaa ccatttggag cgttagatgc tatgaccaga gggcagatgc aagactggat
    18541 gttgaagctg cctaaaaaag caaagcggac tactttactt gtgacgcatg atatcgaaga
    18601 agcaatttat ttatcagaca ggattcttgt gctttccgcc cgtccggcac atgtgattgc
    18661 tgagattcaa gtgcctgaaa aacgacggtc acgtgaatgg ctactgcagc aatcagagtt
    18721 gaagcaaacg atttatcagt tattggcagg tgaattagat gttaagtgat gctcattgtc
    18781 atttagatgg gagtcagagt ttagcactcc ttcagcagga acacacgatt ttgacgatca
    18841 tcaattgtga ttcgccagaa gaatggaagg aaaacagaca gcttgcagca agtaagactc
    18901 aagccttaag ttacggtatc catccttgga aagcagatag ctatacgttt gaacaagttg
    18961 agcctttttt gaaaaaagca aggatcatcg gtgaaatagg gttagataat atttggacga
    19021 atgtaccgat gactacacaa aaaaaagtat ttgagcgaca gcttgctttt gcagccataa
    19081 atgagaaacc tgttgtactg cacacaaaag gatgcgaaaa agagatctta aattatatcc
    19141 aagcttaccc taatcactat ttgatccatt ggtattcttc tttggactat caacaagact
    19201 atattgattt aggctgttat ttcagcattg gtcttgattt gaaaaagaat ccagcagtct
    19261 ggcaactagc taaagcagtc ccaatcgacc acttacttat tgaaacggat ggactacatg
    19321 cagcagagtg ggctttaggg gggatttttt cagaaaaaga ttatgtctca ctgcttgaag
    19381 aacagttaaa gacaatcgct gagatcaaag agctatcagt tgaagaagtt aaaaaaaata
    19441 cttatcaaaa cttggaacgt ttcattagat taggtaaata aaaaagagat gtgacacaac
    19501 tagtgtcgca cctctttttg gctttctttg tataaaagta aactgtttga ttaat
//
LOCUS       DAFBQH010000049        18979 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.051, whole genome shotgun sequence.
ACCESSION   DAFBQH010000049 DAFBQH010000000
VERSION     DAFBQH010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.051"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(94..969)
                     /locus_tag="LK694_001836"
     CDS             complement(94..969)
                     /locus_tag="LK694_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBK5052685.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(1051..1842)
                     /gene="codY"
                     /locus_tag="LK694_001837"
     CDS             complement(1051..1842)
                     /gene="codY"
                     /locus_tag="LK694_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBK5052686.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(1860..3260)
                     /gene="hslU"
                     /locus_tag="LK694_001838"
     CDS             complement(1860..3260)
                     /gene="hslU"
                     /locus_tag="LK694_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBK5052687.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(3274..3822)
                     /gene="hslV"
                     /locus_tag="LK694_001839"
     CDS             complement(3274..3822)
                     /gene="hslV"
                     /locus_tag="LK694_001839"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBK5052688.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(3859..4764)
                     /gene="xerC"
                     /locus_tag="LK694_001840"
     CDS             complement(3859..4764)
                     /gene="xerC"
                     /locus_tag="LK694_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBK5052689.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(4962..6338)
                     /gene="trmFO"
                     /locus_tag="LK694_001841"
     CDS             complement(4962..6338)
                     /gene="trmFO"
                     /locus_tag="LK694_001841"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBK5052690.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(6307..8385)
                     /gene="topA"
                     /locus_tag="LK694_001842"
     CDS             complement(6307..8385)
                     /gene="topA"
                     /locus_tag="LK694_001842"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBK5052691.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(8507..9364)
                     /gene="dprA"
                     /locus_tag="LK694_001843"
     CDS             complement(8507..9364)
                     /gene="dprA"
                     /locus_tag="LK694_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBK5052692.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(9420..10187)
                     /locus_tag="LK694_001844"
     CDS             complement(9420..10187)
                     /locus_tag="LK694_001844"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBK5052693.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(10180..11040)
                     /gene="ylqF"
                     /locus_tag="LK694_001845"
     CDS             complement(10180..11040)
                     /gene="ylqF"
                     /locus_tag="LK694_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBK5052694.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            11300..11698
                     /locus_tag="LK694_001846"
     CDS             11300..11698
                     /locus_tag="LK694_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK5052695.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            11977..12477
                     /locus_tag="LK694_001847"
     CDS             11977..12477
                     /locus_tag="LK694_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBK5052696.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(12560..13111)
                     /gene="lepB"
                     /locus_tag="LK694_001848"
     CDS             complement(12560..13111)
                     /gene="lepB"
                     /locus_tag="LK694_001848"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBK5052697.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(13263..13490)
                     /locus_tag="LK694_001849"
     CDS             complement(13263..13490)
                     /locus_tag="LK694_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBK5052698.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(13487..14005)
                     /gene="msrA"
                     /locus_tag="LK694_001850"
     CDS             complement(13487..14005)
                     /gene="msrA"
                     /locus_tag="LK694_001850"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBK5052699.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(14025..14738)
                     /locus_tag="LK694_001851"
     CDS             complement(14025..14738)
                     /locus_tag="LK694_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBK5052700.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(14741..15637)
                     /locus_tag="LK694_001852"
     CDS             complement(14741..15637)
                     /locus_tag="LK694_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBK5052701.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(15795..16637)
                     /locus_tag="LK694_001853"
     CDS             complement(15795..16637)
                     /locus_tag="LK694_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK5052702.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            16769..17422
                     /locus_tag="LK694_001854"
     CDS             16769..17422
                     /locus_tag="LK694_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBK5052703.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(17485..18012)
                     /locus_tag="LK694_001855"
     CDS             complement(17485..18012)
                     /locus_tag="LK694_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBK5052704.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(18032..18979)
                     /locus_tag="LK694_001856"
     CDS             complement(18032..18979)
                     /locus_tag="LK694_001856"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBK5052705.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
BASE COUNT         5044 a         3971 c         3333 g         6631 t
ORIGIN      
        1 aaatcgatgt cacactccct tattacttac aggatagctt ttttacacac cttggtaaac
       61 ggtgttccct gctaacttct cggtcacaac ctcttacttc actgtgatgg aatattttgt
      121 cttgaagatt tcatgggcac ctagttgatt gatccctact ttttccatca agtttccact
      181 actgttcacc gtgtcagcga ttccgcacca cggttcgata caaacgaaag gcgcttcttt
      241 tggataagga gaccagatcc ctacataagg catatcttta tagcttaaag ttacgctatg
      301 ggaagatttt tcacttcgaa tggcgtacgc atttagaccc cttgtttcaa aaaccaaggc
      361 atcttgctca aaaagttcac gattcaaagc cagattcgta tttgtctgac ctaacgtctt
      421 ttgatccata tcgatgaatg ggcctttcaa tggtaggcgt aatcttgatt tcaatggaga
      481 aaatgccaga tagaaatctt caaatgacaa tccttcttca agaggtacat tgaaagcagg
      541 atgtccaccg atagaaaaga acatttcttt ttcaccggta tttgccactt gataatgaac
      601 cgtgatgccg tctcctccta attcatagct gatcactaat tcaaaatcaa atggatactt
      661 ttctaatgtc tccggcgtac tccttaagat gaaactagcc atttcttgtc catgttcaat
      721 gacttcaaaa tccatatccc gagcaaatcc gtgttgcccc atttgatatg ttttcccttg
      781 ataaacatac tgatcatctt ttaaacgtcc aacaaaaggg aataaaaccg gtgcatgtct
      841 gccccaatac gctggatcac cttgccagat atactctaaa ccatgttctt ttgatttcag
      901 actgatcaat tccgcaccgt gttcagcgat ttcggcgatc agctggtcat tttgaataat
      961 aacagtcatt gccattcctc caacttcgac ttacttttta aaatgggaag gctggaacaa
     1021 acatttctat ctgccccagt ccccttactt ctaataactg tgttttgcta actcgtcttt
     1081 aaaacgggaa ttcaatactt ttaaataagt tcccttcatg cctaacgaac gagattcaat
     1141 gattccagct gattccaatt ttcgtaaagc attgacgatc acagaacgag tgatgccgat
     1201 actatcggca atattagaag cagtcagacg accttcatct ccatccaatg cttcaaagat
     1261 agcttgtaca gccttcaatt cgctataaga taatgtgttg attgccattt ggacagccgt
     1321 tgcactccgt acatctgctt cgatgcttcg agacttctga taaaggatct gcataccgac
     1381 aaccgtagct ccatattctg ctaagactag atcgtcatct gcaaacgatt gttccatacg
     1441 agataagata atcgtaccca aacgttcgcc agctccaaag atcggaacga cagtcgtcag
     1501 accaaatgga tatttctctc ttgactctac cggaaatgca gtaatatcac tagtaattgg
     1561 gatattagct tcagttttca tcaactgtgc cactgtatca gtataagatt caggaaattg
     1621 tttctcaaca aacattgttt tgattcgttc attgttcaca tcgtgacgtt cattataacc
     1681 tagtaatact ccgtcacttc ctatgatata ggtgttgctg tcaagaatac cacctagtgt
     1741 gacagccatt ttatcgtaag gcaaatctgc ttgcatgtca aaagtattct tttgctgcaa
     1801 aagtgtgttt atcttgcggg ttttcgttag caagtctgtc attgtcgtcc tccgattttt
     1861 tataaaatat agcgacttaa atcttcattt gccgcaattg agccaagttt gtcatccaca
     1921 tatgcttctg tgatcgttat ttctcccatt tgcatatctg gtgcttcaaa taatagatct
     1981 tctagtagtt tttccaaaat ggtatgcagt ctgcgtgcgc cgatattatc tgtgtcacgg
     2041 ttgacatcga aagcgatttg agcgatccgt tcaattgctt cttttgtaaa cgtaactgac
     2101 acattttctg taccgatcaa cgcgacgtat tgttttacta aagcattatt tggttcggtc
     2161 agaatcttca caaaatcttc agctgtcaaa tcatctaatt ctacccgtat cgggaagcgt
     2221 ccttgaagtt ctggaattag atcacttggt ttagacaaat ggaaagctcc tgaagcaatg
     2281 aataggacgt gatctgtttg gatgatcccg tattttgtgt ttacttgtga accttcgaca
     2341 attggcaata tgtctctttg gacgccttct ctggatactt ctccagattg ctgtgattta
     2401 gaagtgatct tatcgatttc atcgataaaa ataattccac tttcttccgc taatttgatt
     2461 gcctcactat ggatatcagc atccttgact aattttgctg attcttcctt gatcaataac
     2521 tctaaagctt ctttgactgt taccgtccgt tccacttttt tcttcggtgt caaagcacct
     2581 agagtttcat ttaggtcgat ccccatttgc tccaatccat tgttcatcat tggtgcaggt
     2641 ttcttttggt cttcaacttc aatggttact tcacgactat ccagtaaacc cttttgtagt
     2701 tgttccaaga ctgtacggcg attgattcga atatcttctg tcacttcttc ttgcggttca
     2761 gcggtatttt gctgactagc attaaaaaga ttcatcattt gttcaaattg attagtggat
     2821 tgtttctgtt cttttttgat accaggtacc aagattttta ccaaacgttt ttctgcattt
     2881 tttttagcac ttgtgtatac acggctgtac tgctcttttt ctacgatttg gatcgcattt
     2941 tctactaaat cccgaaccat tgattctaca tctcttccga cataaccgac ttcagtaaat
     3001 tttgttgctt cgactttgac aaatggtgca ttgacgattt ttgccaaacg tcgtgcgatt
     3061 tctgttttac cgacccctgt aggtccgatc atcagcatat tttttggtgt aatatcttgt
     3121 tgcatttttt catctaactg caaacggcga tagcggttac gcaaagcaac ggcaacagat
     3181 ttttttgctg cgtgctgtcc tacgatatat tcatctaatt cacttacgat ttgtcttggt
     3241 gtttgtttta attcatacat atctactcac ctcttacagt tcttctacga taatattatg
     3301 attagtaaag atacagatat ctgcggcaat atttaacgca ttttctgcaa tttctttcgc
     3361 actcatgtcg ttttgagcat agtttttcat tgctcgggct gcagccaacg caaaattccc
     3421 tcctgatccg atagctaaga taccatcatc tggtgtgata acttctcctg tacctgaaac
     3481 aagaagcatt tctttatcat tcattacgat cagcatcgct tcaagttttt gcatagattg
     3541 ctgtgtccgc cattcttgag ctaattcgac agctgcgcgt tgtagattcc cgttatattc
     3601 gtttagtttg ccttcaaatt tttcttctaa agtaaacgca tctgcaacac ttccggcgaa
     3661 acctacaaca acttctccat tgtagatacg tcgtactttc tttgcagtgc ctttcatgac
     3721 aacagattca cccattgtta cttggccatc tccagccata gcaaatttcc cgtctttttc
     3781 gacggcacag atagtggttg aatgaaatgg tgattcgacc atgtttgttt cctcctaaat
     3841 ttgttctttt ccttattttt acgctcttgg atggaaagaa cgatagtttt tttgtaaact
     3901 ttcttttgtt acatgggcat agatctgggt agtcgataaa ttggcatgtc ctagcaattc
     3961 ttggactgtc cgcaaatctg ccccattatt taataaatga gtagcaaatg tatggcgcag
     4021 catgtgagga tggatatcac tatccaaact gctttttttg atcatttgat tcaagacata
     4081 ttcaattcca gtaggcgtga ttggttttcc atgatgattc acaaaaacga tatcatgctc
     4141 ttcgtgatat tttgtcatca atacctggcg gccttccctg aaatattctt gcagagcatc
     4201 ttgagcaaaa gaacctagag gagcatatct ctctttgttc cctttaccat ggatcaacat
     4261 gacctctgaa tcaaaatcaa tgtctgatac cgacaaactg ctacactcac ttaaacggat
     4321 gcctgacccg taaaggactt ccagtaaagc gcggttcctc aaatccaaag gactatctcc
     4381 tgctacgctg tcaaaaagtg cttgcatttc attttcataa aaaaaacgtg gcagtctcaa
     4441 atttttcttt ttgagatgaa cgtaagaaaa tggattttct tttatcactt cttctttgag
     4501 aagatactga taaaacgaac gtaggctggc tatcttgcga ctgatagaat ttctgctata
     4561 gctaggacga tgatcagcta atgcactcaa atagacacga accgtaaaat attccgcctt
     4621 cagcagatca gcgtctccag attcctccaa aaaatgtttg aaatggagaa tgtcttcttc
     4681 atatgcttct ttcgtttttt ctgaatatcc tctttcgaca atcaaataac gcaaaaattt
     4741 ttgttcccac gtggcttctg tcattttctc accttccttt tgtcccagag aataatttag
     4801 cacagcgttt ataaaaagac aaataaattg tcagaattta accaatattt ctaagtttgt
     4861 tcacaattgg cgcaattcct cacattttgt ttgagaaatt cttaagaaaa acgcaagaaa
     4921 aagagcgaga ctatccgtct caccctcttc atggagtttc cttactcatt tgggattgca
     4981 gttttttcta attctgtttt tattgcctca ttagctttta aagctcgttg tgcaattgct
     5041 tcatatcttg cttttttgtc tcgaatgcgc tctggcagtt caggaaacag accatagttg
     5101 gcattcatcg gttggaaatg ttttccttcc gcatgagtaa tgtagtaagc catacttcca
     5161 atagctgttt cttgtggaaa ctcgactggt tccatttctt ttgctaagcg tgcggcgttg
     5221 attccagcaa gtagtccgct cgctgcactt tcaacatagc cttctacacc agtcatctga
     5281 cctgcaaaga acagatcttc gcgtttcgtt gattggtaag tcggtttcag caattctggc
     5341 gaattcataa agctgtttcg gtgcatcaca ccatagcgaa cgaattcagc attttctaac
     5401 ccaggaatca tccggaaaac acgtttttgc tcgccccatt tcaagtgagt ctgaaagccc
     5461 acgatgttgt ataaagaagc tgcagcattg tcttgacgca gctggataac agcataagga
     5521 cgtttgccag ttttaggatc ttccaatcca actggtttca taggtccgaa aagcatcgtt
     5581 ttgaatcctc gctgtgccat tacttcaatt ggcatacatc cttcaaaata tttttctttc
     5641 tcaaaagatt taagttctgc cacttcggca ttgattagtg cctcatggaa agctttgaat
     5701 tcctcttctg tcatcggaca gttcaagtaa gctgcttctc ctttgtcata gcgggacttc
     5761 aaatatactt tatccatatc gatcgtagct ttgtctacga ttggtgcagc tgcatcatag
     5821 aaataaaaac catctgaacc attgaatgct ttgatgcttt ctgctaatgg atgagaagtc
     5881 aaaggtcctg ttgctactac ggtgattcct tccggtattt cagtaatttc ttcgtttatc
     5941 actgttacta atggatgctc tttgactttt cgggtaattg tttcagaaaa agaatcgcgg
     6001 tcaacagcta aggcaccacc agctggcact gctgtttcat ctgccgaagt gattactacc
     6061 gaattcaatc ggcgcatttc ttctttcaat aaccctactg cattagccaa actgtttcct
     6121 cttaatgaat tggaacatac tagttcagca aaattacttg tgtgatgcgc ctcagtcgat
     6181 ttgactggac gcatttcata taaacggaca gggactcctg cttgtgcgat ctgccaagca
     6241 gcttcacttc cggcaagccc tgctccaaca acatttacca ttttcaatga aagaacctct
     6301 tttctattat ttttggatgt cttcttcgta atctccattt acacagacta cttgtttacc
     6361 gcctttgact ttcttctcga ccagatatcc gtcacatttt ggacaatttc tgcccactgg
     6421 tttatcccaa gaagtgaatt cacattctgg gaaacgggaa caaccataga atagtctgtt
     6481 tttcttagat tttcgttcaa tgacttgtcc ttcatgacat actgggcaag tcacaccgat
     6541 ttcttttacg attggtttcg tattccgaca atcaggaaaa ttactgcaag cataaaactt
     6601 cccatatttc cctaatttta tcaccatcgg atgtccacag acatcacaat cgaatcctgc
     6661 tggttcatct ttgatttgaa ttttttcgat tttttcttcc gcattcgtca gttctttttc
     6721 aaacggttta tagaaacgat caaccacttt gacccacgct tccgtgcctt cttcaatttt
     6781 atccaaatct ccttccattt ctgctgtgaa atggatatcg acgatttgag ggaagaattc
     6841 acagattaat gaattgacaa tttctcctag ttcagtcggc tcaaatcttt tttgtgtcaa
     6901 ttttacatag tagcgtcttt ggatcgtatc aagtgttggt gcataagtgg aaggtcgccc
     6961 taccccgttt tcttctagtg cacgaatcaa cgtagcctca ctaaaacgtg ctggtggttg
     7021 agtaaaatgt tgttttggtt caatatcaac agattggact acatcgcctt cttcaagttc
     7081 tggaagaata ttttctttct cttctttgcc gtcatctcta ccttccacat acacttgcat
     7141 gaatcctttg aatttgattt ttgaaccgtt cgcaatgaag atgacaccat tttgttctaa
     7201 tgtcactttc attgtatcta aaatagctgg tgtcatctga ctagccacaa aacgtgacca
     7261 gatcaatgta taaagtttca attgatcttt atctaaatat tgtttgagtt catctggtgt
     7321 acgaagcaca ctcgttggac gcacagcttc atgggcatct tgtgcgcctt gagcattttt
     7381 gatctttcgg tgatcatgtc tagaaaattc ttttccatac gtatcttcaa taaatgctgc
     7441 ggcctctgct tttgctgtat cggcaatccg ggtagaatca gtacgcatgt atgtaatcaa
     7501 gccgactgtt ccttgtttac ccaaagtgat tccttcatat aattgctgtg ctaccatcat
     7561 ggtttttctt gttcgaaagt ttaacttacg ggcagcttct tgttgcaggc tacttgtagt
     7621 gaaaggattc gcagggtttc ttttgcgctc tttcttttct acttttttaa cttcgaagtc
     7681 tgttccatca attctagcag tgacttcttt tacactttgt gcatctggta atttcttttt
     7741 cttaccgtcg attccccaga aatttgcttt gaatttcttg cgagcttttt taaaattacc
     7801 gtcgatgctc caatattctt ctggaacaaa ttctcggatc tctttttcac ggtcaataat
     7861 aatttttaaa gcgacagatt ggacacgtcc tgcacttaac cctttcttga ccttacgcca
     7921 gagaatagga ctgatcgaat aaccaacgat tctgtctaag gtacgacgag cttgctgtgc
     7981 atctactaaa tctacatcga tcgttcttgg ttccttaaaa gctgctttga ctgcgtcttt
     8041 tgtgatttca ttgaagacca cccgattttt ttctttcaga tccaacccaa gtaggtacgc
     8101 taaatgccaa gcaatcgctt ctccttctct atccgggtca cttgccaagt aaactttttg
     8161 agcttttttt gctgcggctt tcaggctttt gatgacatcg ccttttccgc gtatagaaat
     8221 ataatggggt tcataattgt tttcaaaatc gatacccatt ttacttttcg gtaaatctct
     8281 tatatggcca acactggcca ttactttata gtttcgtcca agatatttct caattgtctt
     8341 ggcttttgca ggtgattcta caatcactaa atatttatac gccattactg aatggactcc
     8401 tttccattgt cgtgttccta ctattaaaag aggattcata caaatcgtag ctaatcatat
     8461 ccattcagaa aacgtttgtc aagaaaagca aatgaaaagt atggtgttat tctttaaatt
     8521 ctgccaagat atcttcaccc gaaaccgtgc aaactgcacc gtcttggatc aaatgatgac
     8581 atccggtaga atgttctgtt aaaatatttc ctggaacaga aaaaactgtt cttccaagat
     8641 ccaatgcttg ctgtgctgtt atcagagagc cgcttttttt cttggcttca aagacacatg
     8701 ttcctttaga caaagctgcg ataatccgat ttctcattgg aaaacaatgc tttttcgggc
     8761 cggattgagg aggatattca ctcaaaagaa gatgatcatt tgcaatcacc tcttgcaagt
     8821 cacgattttc agcaggatag acttgctcta tccctgttcc aatgactgcg attgtccgtc
     8881 caccatgaga gattgctgct tggtgaccgt atgtatctat ccctttcgcg cagccactga
     8941 cgatcacata tccagcttct atcactggtt ttagtaaata ataaactgtt tttttggcta
     9001 aagcagaagt gtgtcttcca ccaacaacgc ttaacatgtt acaagaaagt aaagatagat
     9061 caccattata aaacagtaca attggcggat aggtcaagtg aagcagctga gggggatatt
     9121 tgggatcaaa gcaagtgata aacgactgtt tcgattggat tttgccccag ttagcgtgaa
     9181 tttcctgcca acttttgcga aacagctccc ggtgtttggt tattttacct atcttaatga
     9241 tgtcttcaaa tccgatttct ttataaggat actgcatcat ccagttagca agtgcccatt
     9301 ttccttgact actgattcct tttgtataag caagtttcaa taaattttct tctatttgat
     9361 acatcataaa accgcctttc gtctctcttt ctaataagat acgtaaaagc ggctgttttt
     9421 tattgtaaat atttttttac aggtgcaaaa cttttacgat ggattggcgt aattccttgt
     9481 cgttcaagtc cttctaagtg ttcctttgtt ccataacctg cattatgcga gaaaccatat
     9541 ccaggataaa gcacgtcata ttctttcatc aattggtctc gatatacttt agcaatgata
     9601 cttgcagcag caatcgacac agatcgtgca tcccctttga ttattttttc ctgctgaata
     9661 ggcaaatcca aggtcatcgc atcgatcaac aaatagcttg gtacaatgga caatttttct
     9721 actgcttgga tcatcgctaa tttacttgct tcataaatat tcacatcatc gattgtctgc
     9781 tgatcaacga tcccgatccc gattgccagg gctttttctt gaacttgttg ataaagaagt
     9841 tctcgctttt tctctgataa ttgtttggag tcattcagcc ctaatatgat ctcatttttc
     9901 ggcaagataa ctgcagctgc cacgacaggt ccagctaatg gtcctcttcc tacttcatca
     9961 atgccggcaa tccattggtg accttgtgcg tacccttgct tttcaaagca aaacatttct
    10021 tcatattttt ccttaattaa tgcttccttt ttcaatcgtc gttcaaaaga agccagtgct
    10081 tgctgcacgc ctttgcgtgg atcatttctt aatgattcta gtgtaggatc atctattctc
    10141 tgtatgcttt tcaatcgttc tttgatactt tggatggatt cagtcatcac ttttttgctc
    10201 catttcttcg caacgatcca gtgtgtatcg tccaagtttt ccttgacgaa tatcatgcac
    10261 gactaattcg ctgccacgat catagtcttc tttataccca cgttttgctg taatatccat
    10321 cagtaattcc ggtgccaatt tttcttcttg ctcgattgat aaaccataac gatcgctgat
    10381 tcttcctgaa taatatcggg caaaaaagtc taatccgtaa atcgctaaat catctaaatg
    10441 gacaagctgg tctttgatcg cgcctgtcag cgctagtttt ttgccgattt cttcatcttc
    10501 aaatttgggc cataaaatcc ctggtgtgtc taacaattcc aactcattac ctaaacgaag
    10561 ccactgctga ccttttgtta cgccaggctt atttcctgtc tgtgcaattt tcttgcctac
    10621 tagtcgattc atcaacgtag atttcccaac atttggaata cctaagacca ttgcccgaat
    10681 agcgcgaggc ttgatccccc gagcagcatc tcttgcccgt ttttctttca atgcttcttt
    10741 agccgcaggt actagacctt taattccttt actttcttgt gccgtgatag ctaatgcaag
    10801 atggccttgt ttacgaaaat attcttgcca cagctttgtc tgttgtggat ctgccagatc
    10861 cgctttattc aataaaacaa ggcgaggttt ttgctgaatg atctgatcta gcattggatt
    10921 acgagaagat aaaggtaatc gtgcgtctac caattcaaat acgatgtcca catatttcat
    10981 tttttcagaa acttctcgtc gggcttttgc catatgtccc ggaaaccatt ggatggtcat
    11041 tgttctctcc attcctttca taccggaata ttccccggct ttacttgttt ttttcttttt
    11101 caatataaat tcactttttt attataaaga aattccatct attagttaag tgaaagtgaa
    11161 ccagtcctca ttctatcaca tcttcacttt tttgatgatg caaattttta ttttggtttc
    11221 tatatatgaa tctaaatttt tgacagcgtt tcctaaataa gagatgatag acgaacaaaa
    11281 cagaaaggag acgaaacaaa tgaatagtat ggtttttgtt aattttcctg ttgctgatgt
    11341 caatcgttcc gttgcttttt atgaaaagct gggttttaaa aaaaatgaag aattttctac
    11401 tgaagaagct ggttcaatgg tatgggatga cactttttgg ataatgcttc tctctcatga
    11461 tttttataaa aaatttataa aagataaaga aatagctgat aataaaaaaa caagtggtgc
    11521 tttgatttcg tttagtgttg aaaatgcgga tgaagtaaaa aaatttgctc aaattgcaaa
    11581 agaaaatggt ggtactttcc acgaagtaga tatgggaatg cctgaagaag aaatgttttc
    11641 attagaagtc caagatctag acggaaatac attagaaccc gtatggatga agatgtaagc
    11701 agataactaa tctgacgttt tttataacaa aaaaacaaag gcggatgata cgataagtat
    11761 tctccgcctc tatttttttt attcgtacaa tctctttttt acagtttata aaagcagtct
    11821 tctcaccctt tttttaattg ttagatgcca gtgtatgtaa atcaccaatg tgtcatgaca
    11881 cattggtgat tttatggtat ccttttatta ttaactgaca aaggagagaa tattttgaac
    11941 actttgattg caaaagacgt ttttagattg cccgaaatga atgcaagatt gttgtcgacc
    12001 tctgctcttt tattagccct tgatgtcatc ttatacaaga tagcgattgg tcctgcttat
    12061 tttcaaatca cattcggatt tttaagtatg gcgcttatgg gttatctata tggaccgatc
    12121 tgggctggtt tgcttgaaat gttgtcgaac actttaaatt tcacgatatt tggttcagga
    12181 acgtttcaag ctgcttttct tatcacggct ttccttggtg gatttattaa tggcttattt
    12241 ttatatcata aaaagataaa cctgccttcc gtcatcgctg cccaattatt gattatgatc
    12301 ccagtcagcc tttttatcaa ttcttggctg atgtctgttt tgtttggtgc agatttcaaa
    12361 gtaatttttc taggtagatt catcaggaat atgatccaaa tccctattca aataatggtt
    12421 gtttattcat ttatcaaaac tttagaggct agaaaagtgt ttaaacgagc tgtttgattt
    12481 tacgtactaa ggtagtaaac tgattttgga ttaaacaata aagcgtgcag tctcaacaga
    12541 ctgcacgctt tattgtttct cactggatcc attttatttc atcaagtgga taatagacaa
    12601 atcttgcttt acctagaata tcagttttat tgatcgtgcc aaacgaacga ctatcttttg
    12661 aaactcttcg attatctcca agtacaaaat aattatcttc cggaagcttg gattgtcccg
    12721 ttagctcttg tagtgtaaag tccgtcgtat aggatgcatt tgcatgatct gatttcaggt
    12781 ttttagttag ataaggttcc ttaactactt tcccattgat cttcaattga tcattttggt
    12841 agctgacatt ttctcctgga agaccgatta ctcgtttgat ataaatcgtg ccatctgcta
    12901 attgaaaaac aacaatatca aatcgtctga tctccgaaaa tttttccatc acaaccatat
    12961 caccttgctt caacacgttt tccattgaat tgccttccac tggtaccggg atgaataaaa
    13021 atccccgcaa catgaacgca aggaatacag aaacaagtaa gtatttgaat acaagccaga
    13081 agcgattaat atagcgttgt ttttttgtca atctctatca atccatccct ctttttttca
    13141 ttctttcttt attataagaa aaatatcaaa aaaatgcgac tagatagctg aaataaaaat
    13201 aattctttca attaggctat tggacaaaaa acttttttga tcataaagta caaaactatc
    13261 tcttatatgt catgattatt ttctaaatat ttttcccatg cttcgtcaaa gatatccatg
    13321 gacggcaaat agtcgacatt cagttccaga taattagaaa tttcttcgta gctagagctg
    13381 tgcttaggaa attggatgtc tttaccggct tctgtagcga aaacagataa agcatcatgt
    13441 gtgccaccgc ctcgcagtgt cattaaataa tgaaaaaagc ttcgattcac ccttcgttcc
    13501 tccaattttc ttcgataaag gcagcccggc gtgcatggtt tcttttaaaa ttttcaggat
    13561 gctttttata atactcttga tggtcttctt ctgctagata aaatggttgg gctggttcga
    13621 tttttgtaac gattggctgt tcgaaacgtc cactttcagc taactgtttc ttactttttt
    13681 cagcaatgat ccgctgttct tctgtcaaat aaaagatcac cgggcgataa ttgtcaccgc
    13741 gatcctcgaa ttgtccgaaa gcgtcagttg gatcagtttg ctgccagtaa agttctacta
    13801 attgttcata tgaaatcaat gttggatcat agataatctc cactgcttct gtatgtccag
    13861 tcgtttggct ttttacttgt tcataagtcg gatctggtac atgccctcca gtatatcctg
    13921 acatcactgt atggatacca ggaagcgtgt cgaaaggctg gatcatacac cagaagcagc
    13981 ctcctgcaaa tattgctcgt tccattatga ttccccctct aatactactc atccgatgat
    14041 tttttatttt cttttgtctt gctttcagta ctgctggtgc tagttttgtt ggtatttgag
    14101 ttgtttgaag catcagaggt atcttttggc aaatatagac taaaccgaat gtcatcattt
    14161 actaagtcga ttttatctgc tttaatatac atcccgtttt gcattcggaa ttgatccaac
    14221 ctaagcatga tagtcagatc atctgtattg acttcgatcc aatctggtaa tttatagttt
    14281 ctcttgatca tattcatcac atctttgatt ggcaaactca atgttccgat agaaagactt
    14341 ctagctttta gttggacatt tccgttttcc atcacatacg gatcaaaata taaatagaaa
    14401 tcgataggaa aacctagtac ttcgaaagta ccattgagta gtgcttcatt ttccaaataa
    14461 aatttatatt caatatcact atctttttga tactcactaa ggaaaaagct gatgatttga
    14521 ttgacttttt ctttatttga attgatcgtt aagacaggat ctccttgtct ttccgtaaga
    14581 gctggtattt ctgggagttc tggttcccga ttagcaaaaa tacggctgcc taaaaatacg
    14641 gtcgaaccaa gtactacccc aagaaggata aggaaggcga atttccagta atttgttttc
    14701 tttgttttgt ttgaagatac gtcagatctt gttttcatgt ttattgatca tcctccttct
    14761 ctaaccattg gttttgtgtc tcgatcagct gatctctcac tgcatttgcc atcaattgat
    14821 aacctaaatt atttggatgg aaatgatctt cttcatagag cacattattt ttgatgccat
    14881 ttagattttc tgtcgtgcta gttgtttgat ctgttccttc ttctgtgaca cctacttcct
    14941 gattcaatcc ttgataaagc aaatcattga ttgggataaa gtagcagttc ggcatttttt
    15001 ttgtcaccgt ttctgttcct ttgttccaat tgttaacaat tgtctgcatg tcagtgatct
    15061 caggaaagtt caaataaaat ggattgtaga tgcctaaaat gtaaataggt gcggttttat
    15121 tgagggatcg aaattcttcc aatagttctg tgacattttc ctgatagttt ttcagtggct
    15181 ttttaaagga attgaccgtt aacccgaata aattattttg gataactttc atcaaatcat
    15241 taccgccaac agtcagtgtg ataaaatcag ctgttttgat attgtttcga atttcttctt
    15301 ttgtttttac tcgctttaag atctggtcac ttcgttcacc atttacacca tagttttctg
    15361 tttcaaccgc ggtcaaatta taacggtctt ttagatcatt tgcgacaagg ggaacgaatc
    15421 ctccttgctt ggtttgatct cctactcctt cagtcagcga atctccgata gctatgtagt
    15481 gaagtttttc ttttttattt ggatcttctc tcacagtagt tactggtttc attagtgggc
    15541 cagctttcgg tatagaagca gctaaacctg catagatgat gatcgcaatt aataatggga
    15601 acaaaacgag gatcaaatgt cgtttcgtta atttcatttt ttcacctctt gtattcgatt
    15661 ggttctaatg ttatcaaaaa ttttatcaaa aaggtagtct ttatgaaaaa agaaaacaat
    15721 tattttccta attttataaa gaaaaagaga ataagctgca agcctttcag gctgagggct
    15781 tatcctcttt tcgactaatc tacataatac atgattgcaa acgcgtcttt accagtatgc
    15841 gtagcgataa ccggattcgt atgcagtaca gggatatgca tgtccgggaa taattcttgc
    15901 aaaccctctt tgaattcttg cgccaattct tttgcatccg catgtgaaat accgatttgt
    15961 cgcacatttg gcaattcttt cagttcattt ttcaaattat taaaccattt atcgaacgtt
    16021 ttctttcctc gccccttagt tacagggatt aattcagaat gggcgaaatt catcaccacg
    16081 cgtatgttca aaacactaga cagcaaacca gttgttcggc tgattcttcc accttttacc
    16141 aagttatcta gtgtggatat tccgatgtat aattttgtat tgtcttgtac ttttttgact
    16201 tcttcaagga ttttctctac tgttttacct tcttttgcta attcagctgc tcgaattacc
    16261 tgaaaagata atccttggtc agtggtatca ctgtcgataa ccgttacttt cgtttttgtt
    16321 agattactgg cttgtcttgc tgcttctact gttccactca agccttttgt catatggatc
    16381 gaaatcactt cactgccgtc ttctcctagc tcatcataca tcgagatgaa ttctccaatt
    16441 ggtggttggc ttgttttggg cagatttttt gctttcgcca tcatttccat aaatctttcg
    16501 ccgtctagtt ggtcatcatc gggatatact gttccgtcga tcatgatgga tagcggcatg
    16561 acacgaatgc ctaaagcatc gcgtacattt ttttccatcg tacatgaaga atctgttacg
    16621 atttttacat ttgtcattat tttctctctc tctttcttta acaagatgcc ttattcatgt
    16681 taaactacat gtaagacaat ttctattttt agtataacag acaaataatg atttttcatt
    16741 aattacacct agtaaaggag ccgttttcat ggaaaaaacg aaatttaatc gaacgtatga
    16801 aatattaaat gaagtgttta atgcagtcac ccatggaatt gggactggat tgagtatcgc
    16861 gggactagtg gttcttttag taaaaggtgc ccacttgcat tcctccctcc atattgtctc
    16921 ttatgctatc tatggatcga tgatgattct tttattttta acgtctactt tgtttcattc
    16981 attgattttt acaaaagcaa aaaaggtgtt ccaagtattc gatcatagtt cgatttttct
    17041 tttgattgca ggaagttata ctcctttttg tttgctgagt atccgaggat ggctaggttg
    17101 gactttattt gtattaatct ggttgtttgc gattcttggc attatatata aagcgttgac
    17161 gttgcataaa cgggagagag tatcaaaagt atctacgatc atctatattt ttatggggtg
    17221 gctctgtgtc gtggctgctg ttcctcttta tcactctctt ggaatgactg gtgttatttt
    17281 gatggtagca ggcgggcttt cttacacgat aggcgcattc ttttatagct tgaattctat
    17341 ccgctatatg catgttgttt ggcatttgtt tgttatgtta ggtgcaggtt ttatgtactt
    17401 ctctgtttta ttctttactt aataaaaaaa ataaagctat ggatgggaca gctctaaata
    17461 acagctagtc tatccatagc ttttttatat cgtcaattcg cctgtcccgc tttttacagg
    17521 attctcatta cgctgatacg tttcaaattg atgcggataa cgattttttt catccatttc
    17581 tccttgacta gtattgatca gtgaccaatc agaccagtca acaggtggaa aataagcatc
    17641 tccatcgaat acatgatgga tgactgtacg gtataatatc gtacaaaatg gcagaaattc
    17701 ttgataaaca gctgaccctc cagcgatgaa aaagataccg tctgtttctt tttccagagc
    17761 aagcacgtct tccacactat gcatgacatg cacgccttct gctttatagg aagaatctct
    17821 cgtcaaaaca atcgtctgac gattcggcaa cggacggcta cccatccctt cgaatgtttt
    17881 tcgtcccatc actagtgtat ttgcttctgt catttgtttg aagaatttca gatcatttgg
    17941 caaacgccaa ggaagacggt cttcttttcc aatcaatccg ttttcatcct gtgcccaaat
    18001 tgcagctaac attttctcat cctttctgat tctaaacagc gataggagct ttgatcgccg
    18061 gatgtgggtc atatccttct aattggatat cttccatctc aaaatcaaat actgactgct
    18121 tttcctgatt caaaactaaa gtcggaaatg cgcgtatttc tcggctcaat tgttctttca
    18181 tttgttcgat atgattgata taaagatgag catctcctaa tgtgtgaaca aattcgccaa
    18241 ccgctaaacc tgtttcatgt gcaattaggt gagtcagcaa cgcataactt gcaatgttga
    18301 acggaacgcc tagaaacaca tccccactgc gttgatacaa ttggcagctt agccgtccct
    18361 cttgaacata aaattgaaac atcgtatgac aaggaggcaa agccatgcta ggaacatctt
    18421 ccggattcca tgcagaaacg atcaatcttc tagaatcagg agtctttttg attgcttcga
    18481 tcacttgttt gatttgatcg ataaagccac cgtcttttgt ttcccagtga cgccattgat
    18541 agccgtagat gtgtccaaga tcgccgtatg tttcagcaaa ggaatcatcg gttaggatct
    18601 tctcgcaaaa aagcttgctc tgctttttat attcttcatt gaattcagga tctgtcaaac
    18661 atctatggcc aaaatcagtc atatctggtc cattatagtc tggacttttg atgtagcgtt
    18721 caaatgccca ttcatcccaa atatggtttt tattttgtaa tagatagcgg atattcgtat
    18781 ctccttttaa aaaccaaagc aattcgcttt tgatcaatcc aaaaggtact cgtttcgttg
    18841 ttaacaaagg aaaccctttt tgcagatcaa accgcatttg gtatccgaat aagcttaatg
    18901 tgcctgtgcc tgttctgtct tctttacgat gcccttcttc caacaatttt cttcctaagt
    18961 caagatacgc ttgttccat
//
LOCUS       DAFBQH010000050        18773 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.052, whole genome shotgun sequence.
ACCESSION   DAFBQH010000050 DAFBQH010000000
VERSION     DAFBQH010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.052"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(565..1131)
                     /locus_tag="LK694_001857"
     CDS             complement(565..1131)
                     /locus_tag="LK694_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK5052706.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1131..2087)
                     /locus_tag="LK694_001858"
     CDS             complement(1131..2087)
                     /locus_tag="LK694_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBK5052707.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2087..2743)
                     /locus_tag="LK694_001859"
     CDS             complement(2087..2743)
                     /locus_tag="LK694_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBK5052708.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3113..5131
                     /locus_tag="LK694_001860"
     CDS             3113..5131
                     /locus_tag="LK694_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK5052709.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5201..5407
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5558..7972
                     /locus_tag="LK694_001861"
     CDS             5558..7972
                     /locus_tag="LK694_001861"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBK5052710.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8320..9873
                     /locus_tag="LK694_001862"
     CDS             8320..9873
                     /locus_tag="LK694_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBK5052711.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10254..12038)
                     /locus_tag="LK694_001863"
     CDS             complement(10254..12038)
                     /locus_tag="LK694_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5052712.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12053..13774)
                     /locus_tag="LK694_001864"
     CDS             complement(12053..13774)
                     /locus_tag="LK694_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5052713.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13874..14635
                     /locus_tag="LK694_001865"
     CDS             13874..14635
                     /locus_tag="LK694_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK5052714.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15602..15870
                     /locus_tag="LK694_001866"
                     /pseudo
     CDS             15602..15870
                     /locus_tag="LK694_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16542..>18773
                     /locus_tag="LK694_001867"
     CDS             16542..>18773
                     /locus_tag="LK694_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052715.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6173 a         3329 c         3772 g         5499 t
ORIGIN      
        1 tttattttcg gaaaaagcta atatagctaa gattgaaaca tccatctgga atttatcttt
       61 tttactgctt tgatcataaa agtctgcata ttcatcactg atattttttc taataaattc
      121 ttccatcata aaaatcacct caaaacgatt atacgaactt acgttcttgt tgtaaagcga
      181 tatttgaggg gcaaaaaagg ggcataattt gactgatatt aagttagcta ttgatatatg
      241 aatgacatac aaaaatataa aatcttttaa aaatgcttat atatcaatgt ttttgccatt
      301 cttttatata tctgttatat gtcaaaaaaa tgtgttcaat ttcaatttgt atttgaataa
      361 agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaatca aaaacagctt
      421 ctcatgtttt tgatttggtt ggacttatta ccgcagatat aagcttttga gcaccgtgaa
      481 taaagaggtt atgaaatcaa cgctctaacc tgagtattag agaaaaaaac gcaaaacata
      541 tcatttatgt tttgcgtttt ttatttatct ttttttctca atacaatata aaataggcgg
      601 ctggttcttt tggttgataa accgataatt cagcacatta tatgtttcct gtggcagttt
      661 ggaacaaaaa gaatcaacca tatccagttc tttttctcca ccttcatgac catagtagac
      721 gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat atttcttcca tagctgtgcg
      781 tgtagtctct ggcatagtga tgattgcttt atcacttttt gggagatacc ctagattgaa
      841 aatccctgct ttgagatttt gttgttcagc tatcacttct cctaacgttt cgtggccttg
      901 tagaaacaat gagacacgtt cttctaactg acgctgttct agcctttcac gagtagcatc
      961 gattgcttgt tgctggatat caaaactata gacatgacca gtttttccga tgtgttctgc
     1021 tagaaaaacc gtatcgtggc cattgcccat cgttgcatct acgacgtgat cccctggttg
     1081 caagatttct tgcagtaatg tatggctgaa atgcaaagca gtttgcaaca tcagacgctc
     1141 acctttttcc gtacatcata ttttccttgg aagctatctc tgcgaagaag ttcttcatca
     1201 attgcattca agacttccca ttttttcaag ctccacattg gtccaatcaa ggaatcccac
     1261 ggcgcatccc ctgtcaaacg atggatgatg atctcttttg gtatcatttc cagctggtcg
     1321 caaatgacat tgacatagtc aggacggctc attaattgaa ggcgaccttc atgatagtcc
     1381 cgcaacattc tcgttttgcg catcagatgc atcaaatgga gcttgattcc ttggatatct
     1441 gaatctaaaa tggtgcgtct gacattttct ttcatcattt ctaaactttc gcctggtaat
     1501 ccgttgatca aatgcgtaca aacacggata ttatgtttgc gtaatttagc cacgccatct
     1561 aaataagttt gataatcatg tgctcggttg atcagttttg aagtttcttc aaaagttgtt
     1621 tgcaatccaa gttctaccca taaatacatc cgctcgttca gttcagcaag gtaatcaact
     1681 acttcatctg gcagacaatc cggtcgtgta ccgactgata atcctacgac acctggtaaa
     1741 ttgactactt gctcaaatcg ttctttgatt atttcaactg gcgcatgggt gtttgtaaaa
     1801 ttttggaaat aaacgatgta ttgttggaca tgaggccatt tttgatgcat catgtcgatt
     1861 tctttctgga attggattgg aagagggtct tcaggtgcaa cgatcatgtc tcccgaacca
     1921 gatacactgc aaaaagtaca accgccatgg gcaacagtcc catctcgatt cggacagtca
     1981 aatcctccat cgatcggcac cttgaaaatt ttcccgccaa actcatttcg taaagcgtaa
     2041 ttccaagaat gatatctttt atttccttct gaataaggaa aagtcattaa aagtccttct
     2101 ttctgtaaga gttatctatt tgttttcttt tttctctata cactggataa gtcaggcaca
     2161 gccaagaaag actgagtaag tagccgccga tgatatcagt aggaaaatga acgcccagat
     2221 aaatgcggct tatcccgaca cttactataa taaacatcat cagcaattga agcaatgtgc
     2281 gtaaggttct tttttgaaca aaagctggaa gcagaaatag taaagaaccg taaagaatca
     2341 tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt ttcaactact aagtgcgcca
     2401 atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg gttgatgatt ccagcaatta
     2461 caataaatcc agaaacgaac caaaatgttt ctgccctttt tttcttatac cacatccata
     2521 aagccgcaca agcaaataaa atgatgacgg taagaggatt accgaattta gtcataaaga
     2581 gttggtagct gttcagatca ggataagaag ctctgacaat gcctgtaaac cactgatcga
     2641 aaccagaaag ccacgacaga tggaacttta cgatccagcc caaaatgaaa aacaggacaa
     2701 gaaagaagct accagccacc tgaaatgatg ttttatattt catacaattc ctcctttgaa
     2761 gcaatgactt attatagcat ttttggtcca gcgaaaaaaa cggtaaagag ataaaaccaa
     2821 tgtatgattc ctctttgaat aaccaatatt cttataaaaa aaataactgt aatcggaaag
     2881 acattttttg gtaatgcaaa tgaaatgtta gggaaataaa atcagtgttt ttcgaaatcc
     2941 ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg aacagaagtt gttcattacg
     3001 gtaaattaca atatgattac aaaaaacaac aaagctctta aataactttt aataaatatt
     3061 aagagaagtt ataattatta ataatagata aattgaagtt ggaggataga agatggaaaa
     3121 cattgcaaga aaagaacgga gacgtttaga acagaccaaa cgttttcgaa aagcaaaacg
     3181 aggggcagca atcgtgggaa ctgcgatggt aggctgttct gtcgctgccc cattagtaca
     3241 gccggttcct gttcaagcgg atgaaacacc tgttcagttc ggcgcaagaa tcaatacagc
     3301 ggcatttatc acagaaattg ctacttatgc tcaaccaatc gctcaagcca acgatctata
     3361 tgcatcagtg atgatcgcac aagcagttgt agaaagcggt tggggcagta gtgcgttatc
     3421 tcaagcacct tattataatc tttttgggat aaaaggcagc tatcaaggac aaaccgtcta
     3481 catggatacg cttgaatttt taaataatca atgggtaaat aaaaaggagc ctttccgaca
     3541 atatccttct tttgctgaat ctttcaatga caatgcctat gtacttagaa atacttcatt
     3601 tggcaacggt tattactatg ccggtacttg gaagagcaac acgaaatcct atacagatgc
     3661 gacagcttgt ttgacagggc gttatgcgac tgatcctggt tacgcaggca aattaaataa
     3721 tatcatcgca acttacggat tgacccaata tgatacacca gctagcggaa acgcaggcgg
     3781 tggagcgact acaggtggca ataatactgg aagtgcaggt agtactggcg gttcaagcaa
     3841 ttctggtaat acaaactcag gttcagcaac ttcgaccact tatactgtcc aatctggtga
     3901 ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg gatcaattga tccaatggaa
     3961 caacatccaa aacaatttca tttatccagg acaaaaactg acaatcaaag gtagccagga
     4021 aaatggctct tcgacgaata attccggtaa caataccaac tcttcaggaa atgctggtac
     4081 atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt caatctggtg attctgtttg
     4141 gaagatctca aatgatcacg gaattacgat ggctcaattg attgaatgga acaatatcaa
     4201 gaacaatttc gtttatccag gacaacagct aatcgtatca aatggcggtt cttctaatgc
     4261 tggagcaaat actggctcaa atactgggaa tacaagtggc tctagttcgt ccagctcaag
     4321 ttcttctaat acagcttcag gaacgaaata tacagttaaa gccggagact ctgtttggag
     4381 cgtctcaaat aaatacggaa ttacgatggc tcagctaatc caatggaaca atatccaaaa
     4441 taactttatc tacccaggac aacaactgac tgtttcaaac agtggatcaa caaacagcac
     4501 gtcaggtaat acaggttcgt caaccaatac aaataacagc agttcagtta atactggcag
     4561 cagttcgtct caaggcactt ataccgtgaa agcaggtgaa tctgtttgga gcgtggcaaa
     4621 taaaaacggc atcacgatgg atcagttaat cgaatggaat aatatcaaaa acaacttcat
     4681 ttatccagga cagcaattga tcgttaaagg tggaacttcg gcaaatacaa atacaggttc
     4741 aactacttct gcagcgaaac caaatacacc gaatacgtct gctacgacaa gtacatcatc
     4801 cactggtaat acgatgtata ccgtgaaagc gggcgagtca gtttggagcg tagcgaacaa
     4861 acatcatatc acgatggatc agttaatcga atggaataat atcaaaaata atttcatcta
     4921 tccaggacag caagtgattg tgaagaaagg ttcatctcaa tcaacaaacc aacaatcagt
     4981 tactggttcg aaaacctata ctgtgaaatc tggagaatct gtttggagtg ttgctgacag
     5041 tcatggaatt acaatggctc aactaatcga atggaacaat attaagaaca actttattta
     5101 tccaggtcaa acgttgattg tagctaaata aaatagctgt tcgaagaaat aacgattgaa
     5161 ttccacaaaa atatttgtta ctataaggct acttaaatat gacaacactt gaaaaggaag
     5221 tagtaaaaag actgaagttt cagagagcgc atggctggtg agaatgtgca cggatgcttt
     5281 tgaactcgcc tttgagttgc ccttgctgaa tacccgagta agcaagcggc ggtgacggcc
     5341 gttacaacgt tcgaggcctt ttcactaagg taaaattagg tggtaccgcg tagattacgt
     5401 cctataaaaa gagcaatctt tttataggat ttttttattt cttttgtgaa gtttgagaaa
     5461 acaaagcaaa aggaaataaa aataaacaga ttctgccggt tttttcgtta tatttataat
     5521 agaaacaagt gttttcaata gaacaggagg acagcaaatg agttacaatc ataaagaaat
     5581 tgagaaaaaa tggcaaaaat actgggcaaa aacgaatact ttcaacaccc atgatgatcc
     5641 tgaaaagcca aaattttacg cactggatat gtttccctat ccatccggtc aagggctaca
     5701 tgtagggcat cctgaaggtt atacggcaac ggatatcctt tcacgtgtga aaagaagtca
     5761 aggatacaac gtgctgcatc caatggggtg ggatgcattc ggtctgcctg ctgaacaata
     5821 tgcgctagat acaggaaacg atccagctga gtttacccaa aaaaatatcg aaacattccg
     5881 acgccaaatc aattctttag gatttagtta tgactggaat cgcgaagtga atacaactga
     5941 tccggagtat tataaatgga cacaatggat ctttacgaag ctttatgaaa aaggtctagc
     6001 ttatgaagca gaagtagcgg taaactgggt acctgaatta ggaacagtta tttcaaatga
     6061 agaagtgatc gatgggaaaa gtgaacgtgg tggctatgat gtagtcagaa aaccaatgcg
     6121 ccaatggatg ctgaaaatca ctgcttatgc tgatcgctta ttggatgatc tagaattagt
     6181 tgattggcca gaaagcatta aagaaatgca aagaaattgg atcggtcgtt cagtaggagc
     6241 aaatattgaa tttaaagtgg ctggtacgga taaatcctat accgtattta cgacacgacc
     6301 agatactcta ttcggtgcaa cttactcggt tcttgcacct gaactagatc ttgttagaga
     6361 aattacgacc cctgaacaaa aagctgctgt agaagcttat atcgaagaga cagctaaaaa
     6421 atcagatcta aagagaacgg atcttgcaaa agaaaaaaca ggagtattta ctggtgctta
     6481 tgcaatcaat ccagttaatg gcaaggaaat cccaatctgg atcgccgatt atgtattatc
     6541 ttcatacggc acaggagcca tcatggctgt tccagcacat gatgaacgtg actttgaatt
     6601 cgctcaaact ttcggactag aaattcttcc agttatcgaa ggtggagatg ttgaaaaagc
     6661 tgcttacaca gaagatggta cgcacatcaa ctctgaattt ctagacggca tgaacaaaca
     6721 agaagcaatc gataaaatga atgaatggct agaagaaaat ggcgtaggga aaaaagaagt
     6781 cagctatcgc ttacgtgact ggctattctc ccgtcaacgt tactggggtg aaccaatccc
     6841 aatcatccat tgggaggacg gaacagtcac agctgtccca gaagaagagc taccattacg
     6901 gttgcctaag acaaaaaaca tcaagccaag cggtactgga gaatctccat tagcaaatat
     6961 cgaggagtgg gtcaatgtcg ttgatcctgt aacaggtaaa aaaggccgtc gcgaaacgaa
     7021 tacaatgcca caatgggcag gaagctcatg gtattacctg cgttatatcg accctcataa
     7081 caaaaaagaa ctagcagatt atgaaaagtt gaagcgttgg ctaccagttg acatctacat
     7141 tggtggagca gaacatgcgg tacttcattt actatatgca cgattctggc ataaattctt
     7201 atacgatatc ggtgttgttc ctacaaaaga accattccaa aaactataca atcaaggaat
     7261 gatcttaggc gaaaacaacg aaaaaatgtc aaaatctcgt ggtaatgtag tcaatcctga
     7321 tgatgtggtc gaaacttacg gtgcagatac actgcgcatg tacgaaatgt tcatggggcc
     7381 attagatgct tcgattgctt ggagcgaaaa tggcttagaa ggaagccgta aattcttgga
     7441 tcgtgtatgg cgtttgatcg ttgatgagaa caataaaatg cgtgaccgta tcactacatt
     7501 gaatgatggc aagcttgata aagtgtatca tcaaacagtg aaaaaagtga cagaagacta
     7561 tgaaaatctt catttcaata cagcaatctc ccaattgatg gtctttatca atgaagccta
     7621 taaagtggac gctctgcctt atgaatatat cgaaggattt gttcaactac tcgcaccgat
     7681 tgctcctcac atgggtgaag aactatgggc aatccttgga aatgatggtg gtatttctta
     7741 cgcaccatgg ccaacatacg atgaagcagc acttgttgaa gacgaagtag aagttgtttt
     7801 ccaagtaaac ggtaaagttc gtgcgaaaag caatgttccc cgagacttag gaaaagatga
     7861 gctagaaaaa gcagcattag ccaatgaaat agtgcaagaa tatattgaag gcaaaacagt
     7921 cagaaaagtt attgcggtac caaacaagtt agtcaatatc gttgctaatt aattggctct
     7981 ttgtcaataa ggactgatga gttgtgcaaa atcaaatctg ggccagaatg aacttcattc
     8041 tgtctcagat tttttgttat ttcaaattga ttaattgatt gatcaaaaag gttttgattt
     8101 tcatgttctc aaacacgttc aatgtctttt tcttctgaat ggtcttgtaa gagatctctg
     8161 taatcatcaa atttttgtct attaatctta gtattttttg atgtataaac tacctgaaat
     8221 tgagagtaca taccaaattc ataaattttt tattagtggg actagtgatt gtatggattt
     8281 tgacgcataa tgaatgaagt atgagaaaag gagagacaaa tggatcaaga actatttatt
     8341 atcgaagtat tatcagctaa acaaaaaatt atcgagaaag cgaaaaaaag agcgaaaaga
     8401 ataatgttca agcttttaag cttagtattt ttctctggtt tgcttttttt cggagctgta
     8461 tcgttcctat atcgccatat gagtgcagta gtccgttcag ctgaagggat gcaaaaatta
     8521 aataatttgt atcacttttc gtggggagtt ggcggaataa caattgttgt gcttattgtt
     8581 tttcttctac ttatttggct ccgttaccat caattgtcgc gtcatttcga tcaatggctt
     8641 agtcaaagag cacaacagtt tttacataat aaaccaactt ctcaagatga tgactatctt
     8701 tattttgaga agacttcgac gagtcaagct tttcggatac aaaaaagtga ttgccagtta
     8761 ttgctgagat cacttgatga tttacctatc tacttaggcg aaagcaaatc aaaattcgga
     8821 atgaacacat tccatatttt tttactagaa aacgaaccca gatgggaaaa aagccagtct
     8881 tcgcctggtg gatacctgac aactaagatg agtacattag tcactggttt tttcctcatt
     8941 cttttagctg gaacaatcta ttatgggaga tacactcagc caaaaatgtc ttctcatata
     9001 aatgctgctt ctcacatgac aatgaactca actaacgata cgcatataca aaagacaaat
     9061 gaagggttgt tagctgatcc taagcaaaag gtaaatttga caacacaaaa gataaatgat
     9121 ctcaaacttg atacagagac gcactctctc tatatgacaa cagatagtgg tgctaattgg
     9181 gtatttgtgc cgattgattc gcaatggttg cgttttggcg aatacaccct gacaacgggg
     9241 ctgattcctg aaggttattg gatggacaac acatatgata tttccgccga tttctcatgg
     9301 tttatctatt cttctgggga tgatgtacgt ctgctgactt caagagataa tggtaaaaca
     9361 tggcaaacgt ctattatcac ccaatcagtt cagccgatac gttatcgaaa agcggtattt
     9421 tatggcaacg atggtctatt tatctgtaca tcagccagtg ggatgtctgc agaaactttg
     9481 tacatttaca ctactatgga tggcggaatc acttggataa aaagtggaag tacaatgatc
     9541 aatcagccag tacaaaatgt cagcttcgtt acaacttctt taggttttgt ttcgacacgt
     9601 gaaagtttgt tttatacaaa caacggagga cgctcattca aagaatcagt tctgtcgatt
     9661 ccggaaaact atcagacagg ggggatagat ctttttcaat caccaaatga agttactcaa
     9721 ataagcgcga atacgttaga gacaaaattt tatttgttga aaaaaggacc aatcgatgca
     9781 gggaaaatgt ttgcctgcct cttccgttca actgataacg gggaaacatg gcaattcgtt
     9841 cagcaactat cgcaagtaga acaaggagat taaagaagag agattgtgag cctgacgttc
     9901 agcaccgagt cacaagaaac aaattcaaaa aacagcttct catatttgtt gaatagtgta
     9961 actcgtgatt cggagttgac aagagcaaca agaagcgatt ttgaagtata gcttctcgta
    10021 ttttacaaga tcgtgtctta ttgccgatag gttgtctctt gaacaccgtt tattcgtaaa
    10081 aagggattgt gacaagtctt ttgtcactac ttaccgaacg aaactgaagg agctatccaa
    10141 aaagtatcat tagtattatt ctttgtgttt ctagtggcac cactagtaca tttaaaaaag
    10201 ctatcgatca tggactaatc atacatgatc gatagctttt tagataatgt ttgttacgct
    10261 atctgaaatt gactattata aagactggca taaagcgttg gtgattggag tagttcagaa
    10321 tgtgttcctt tttcgacgat atcgccattc ttcattacaa ggatcatgtc tgcattttca
    10381 atcgttgaca aacggtgtgc aatcacaaaa cttgtcctat cttttgtcac agcttccatt
    10441 gcttgctgaa tcaatgcttc tgttcgtgta tctacgctag aagttgcttc atctaaaata
    10501 acgacaggag gattcgctag aatgactcga gcaattgtca ataattgttg ctgtccttga
    10561 gataacgtgc cgttttcgct agaaatgatc gtatcatatc cgtctggcag tgttcgaata
    10621 aagtgatcac attgtgcgat ttcagccgct tcgataattt gttctcttgt ggcatccatt
    10681 ttcccgtagg cgatattatc agctaccgtt ccttcaaaca gccaggtatt ttgtaaaacc
    10741 atcccaaaag aacgacgtag ttctccacgt gaaagcttgg taatatcaag accgtcgaat
    10801 tcgatctttc ctttattcag ttcataaaaa cgcatcagca gattgactaa agtcgttttt
    10861 cctgcaccag ttggaccaac gatcgctacc gtttgttttg gctttactga aaaatcgact
    10921 tgattcatca atattttatc tgatgtgtaa ccaaactgta catttttgaa agctattttt
    10981 cctttcggtt gttctaaaag tcttgcatct ggaaggtctg gaatttcatc tgtttcatct
    11041 aatatctcaa atatcctatc gattgctgcc attgcggatt ggatcgagtt gatcacataa
    11101 gaagcggtag aaattggttc agaaatctga ttgatgtact gaagataagc ttgtaagaag
    11161 ccgatcgtca caccacctga tagaacaagc atagcaccga ataccgcact tatgatgaag
    11221 gcgagttgat taatgaaacg aatggccgga tagatagcaa agttcataaa ttgagcacgt
    11281 ttaaaagcgt tgtattgctt tttatttact tcgtgtatag cagcttctgc atgagcttgc
    11341 tgattgaatg ttttgaccac tagattacca gctaagattt cttccattcg attatttaaa
    11401 acacccagtt catactgatt ctgttcagaa atctgtttgt ttttgttcgc aatcttagtt
    11461 gtaacaattg aactgccaac gatcagcaat aagacgagca aagtaagctt gctttcgata
    11521 taaaacagca tcagaccagc agcagctatt gttacgacag aagtaaaaaa ttgattgata
    11581 cctgtcaata acacttgaga aagttgattg agaccggtag ttgtccgact aatgatgtca
    11641 cctacttgat gattgtcaaa aaacgacatc ggtaatgttt tgaatttttt tgtgatttct
    11701 tttcgcaccc tcaatgtgat cttttcgctt aacgctgcca ttgtccgttc ctgaataaat
    11761 gaagtgatgc tgcttaagac tgaacagccg atcagcaata agacagggaa caacaatgct
    11821 tgtttgatat cactgaaaga aagctgtcca atacctgttt ttcttatttc tgataagaga
    11881 tcatcgatcc cgattcccat gatcaagggc atggctacga ctaataggtt cccgaataaa
    11941 ctgcatagaa acaatccgta aaaatattta tgttcaggtt tgatcatttt ccaaaaacgt
    12001 atcaaagcac gcgtgttcaa cgtttttttc ttttccatcg tatcccttct ttctatattc
    12061 cttgtgattt tgcaaactcc tgataagttt ggcaattaga cagtagttct tcatgagtac
    12121 cttgtccaac gatttttcct tcatctaaaa caatgatgtt atctgcttgt tggattgtac
    12181 tgaggcgttg agcaacaatc acgagagttt tctccgacat gtgtttatga agggctgctc
    12241 ttaatgctgc atctgtttta tagtctaaag cggagaaact atcatcgaat acatagatct
    12301 ctgctggttt gatcaacgct cgagcaatac atatccgctg tttctggccc cctgaataat
    12361 tagaaccgcc ttgagcaaca aagctgtcca atccgtcagg aagggtgtcc actagttctt
    12421 ctaattgagc gatcttgatc gctttagaca actcttctgt tgaggcatat tcatttccca
    12481 tctgcaagtt gcttaaaatt gtcccgctga ataagaaagc cttctgaggg acataactga
    12541 tgtgggagcg gatctgttct tgagtcattt gagtaagagg aataccacca agggttactt
    12601 ttcctatcgt agggtcacta agtctcaaga gaagtttcgc caaactactt tttccagatc
    12661 cggtcccgcc aacgatcgct gttgtttttc ctttaggtat tgtgaaatca atatcttcta
    12721 gaactggatc agagatttca gtatatttaa atgtgacatg ttcaaaagca gctgcatttg
    12781 tctgatcaaa ggttggttca gctactgttt tgatcgtctc ttcctcaatt tcgatctctg
    12841 cttgcagtat ttcttctatc cgtcgtaatg aggcaaacga tttagggata atgaccaata
    12901 ccatagctgc gatcatcaag taagcaagtg tcagcataga gtattcgact acagcagtca
    12961 cgacaccgat ttgtaatgct ccttctgcta caagatttcc gccaaaccac aaaatagaag
    13021 cataaacgat tcccatgagc aaaaaagcaa ttggagtgac ccaagcaaat attcggttca
    13081 ctttgatcat ttgattagca tagtcagcaa agctcttatt cgttcgcttt tcttcatatg
    13141 attgattatc aaaggcacgg atcatattaa tcccagtaaa gaattctctc aatgtgatgg
    13201 tgatccgatc catttttggc tggatactca aagataaagg tgcccctcgt ttcatcagtc
    13261 gataaacaat ccatacataa aaaacaacag agatcaatgt aacccatgtt agtaatagag
    13321 aataacgaaa catcatgaga atggtgaaaa cgcaaataac cggtgctggt aagatcattt
    13381 ggcaaaataa aacaatcatt tgctggacat tatcgacatc attcgtcatt cttgtcaaaa
    13441 gagatccaga actaaaacga ctgatatcct tcattgacag ctgttggaat ttacggaaat
    13501 atttctcccg cgtttggaat ccaaacctag cggaagccaa tgctgaaaag taactagagg
    13561 caactgcagc gattgttcct agaaacgcta caagcagcat ttcaactcca atttttttta
    13621 taactgaggc ttcgccactt gctatacctg tgtcgatcag ctttgctact aattttggta
    13681 cacctaatgt accggcgatt tgtaaacaga tcatcacaaa ggtaaggaga gcgagtcctt
    13741 tgtttttctg taagaatgtt tttaagatag acatattatt ccttcttttt tcctgatact
    13801 tttcgattct ataccttcaa gtaacttgaa ggtcaataga aaaaagaata aactgaaaaa
    13861 gaggtgagaa aaaatgaaaa aaatgtatac gattggccaa gtatcggaac tatttgattt
    13921 gccaaaatca acgatccgct attgggatga acaaggatta atccgttcaa gtagacaaga
    13981 agagaatgat tatcgattgt ttgatatcga tgatattttt atgctttatg atatcgtttt
    14041 ttatagaaaa ttagatatac cgatcaaaca gatgaagaat ttatatggaa aaacattgac
    14101 ggagttatat gagacacttg atgaaacaga aagaagaatc cataaagaac tggtggtgat
    14161 gaaacaaaaa caaaaagaga taagggagca aaaaaaacag ttgaaattga tgatcgatac
    14221 aaacgaggaa gaatttcctg tagaagagat cccttttgac tgtatgatct caacggagtt
    14281 tgaagatatt gtggaaatca aaaaatttct acctaactat tcaagctttg ggatgatgag
    14341 tatgccagct tccagttcac agacgatgta tggatttttt atcgacccta gtgaagtaca
    14401 tttatttact caagacgtga tttgggagaa aaaagatacg gctgtttatc gtcgattctt
    14461 attgaaatcc gagatgaacc atgcggaacg taataatatt ttagaaatca gagaaagaat
    14521 gtatgaaaaa ggatggaaaa caggagaagt gatcggccag tatctattga cgaatacgga
    14581 tgaaaacaat atcagaactg aatattatca tgcatggata gaaatgaaaa aataggaaat
    14641 atgtgtttaa gataactcga aaatgatggt tgttttagtt ataaagaaaa attttctggt
    14701 tcgattacgg gtttaatgtg ggggattatt agtcgtggat agatagaatt ttagtttttt
    14761 agaggaaata tgtctttaaa aatggaatgg ttgcaattct tagcgatata tacgatgtaa
    14821 ttgtcctctt aaaaaagtat ggaaaaataa ttgtattggg tgttatgtat aattcatggt
    14881 gaactgagac taatgagaag aaaaattcat attgttatga gtgagattgt atcagatgag
    14941 aatgataaat taaataggtg cataaaagca ttacacttaa aatacattgt tgcttacatt
    15001 attacaatat gttaataagg caaaaataaa tagatggatt gaataaacat attttttaat
    15061 caaatagtcc ctactgtgac tttcctcagc tatcggtgat actaccgata gcattttttt
    15121 gcttataaaa taattataag tatttagaga tttttcttct aacattcttc aaacaagaaa
    15181 aatcacaaag gttattgtta tttttatata ttctttttaa gaatgattgt ttttttatag
    15241 aaattttaaa aaataaaaca cgttattgat ggatagttag tttgttattt ggtaatatta
    15301 tagaagttga ctggtagtga tttatttttc gtcgttttat aaaaatgtta ttttttggaa
    15361 aaattcacaa atatgtaacg aatgtttttc tggaatttgc tgtgttaaaa agcagtatga
    15421 ctaatataaa tactattgtc atttatgcaa ttttttatgg atagtaattt tagaggggaa
    15481 tcaatgaagg aagaatagaa gggaaaaata gttgcaaata cgccagttag caaaaatagc
    15541 aatacatctt caagctttaa aattcgagag aatcatcttg gtagtggaat caactacaac
    15601 aaaaggatta aaccaattaa tagatagaat taccaatgga aaaatagaaa aggtagttat
    15661 tctatacaaa agttgtttaa ttcgcttcgg ttatgaactt agtgaaaccc tttgcaatat
    15721 atggtacaga atatagaaat tattgacatt acagaaaatg atgaagattt aatacagatt
    15781 gtcactattt gtagatacct tttacaagga aaccgagcca ataaagctaa gaagatgatt
    15841 aaggagttga tggaagacga tacaagttaa gaaagtgaaa ctacgcccaa ataaagagca
    15901 aaaagagtaa ttggacgtgg aaaatgcaag aaatgaacca tcgattcggt ggacagttta
    15961 ttagtaatga cggtttgaga acatccatga cgagaatgaa ggaacatcct aaatgcgttt
    16021 agttgaacca agtgccaaat aatatggcca aataagcgat cagttatcga tgaatcccaa
    16081 gtattctaaa ctatatattc attttgatgc taagcattga tatatttttt tgggattgat
    16141 gaaggttaac caatacaggg actcactgat gaaattctag agtctgacct tggtatcaac
    16201 gatttagtga ttgtttctaa tggaaaagta taaaagtata taagtacatt aacaaaataa
    16261 ctattagggt tacgctccag cttgtgcaag gcactggcag gaggaatcgt gaaatcataa
    16321 ttatggttaa gccacacgga caactaaaag tcatacaaag ctatcgaatc aatcaacaga
    16381 agacaatcgg caatcattag ctttgtttac agcttacatt gcaacgcttg agttgaaccg
    16441 aaaatacgag ggaggtaacg cagccatttt gagttgggga atctttttag aaaattatcg
    16501 ggtaccttgt tgtttagtca aaattataag ggggagtaat agtgaggaac aaatttcata
    16561 aactgttaag tatgtttcta gttatgctta ttgtgatcaa tcaatttggg aacataacgg
    16621 ttctagctga gtctttagca gaacagccag agcgttcgct gacgatcgaa gttgatcaag
    16681 aggaggctgc tttgggtgag cagttccagt tgcgagtcag aggaacacca gacctgctaa
    16741 atgcattggt cattgataag agtgaggatt tgattgaagg ggaagtgaag caactcaatg
    16801 attacgaacg tctgattact tattcttccc agcattcagg taaatacgaa ttgaccgcaa
    16861 gaagcggtga tattcaatca aatacggtct caatagaaat cagtgtacca gaagaaagca
    16921 caagtgagtc ttttaatgta ccagaagaaa gcacaagtga atcttttaat ataccagaag
    16981 aaagcttaag tgaatctttt aatgaagcca gatcatctga gccatctata acgcaggaat
    17041 ctcagattga aagacaagag gagctcttga attatgacat cggcatctct gctgagcgcc
    17101 ccactttgcc gagcaggcat acacaaacgt taaagggata cattcaagga ggcgctggcg
    17161 atattcttga agaatatggg gagatggtcg tacgagttcc tagagcatcg attggcttaa
    17221 ttgagttcat tagagtggag gtcgctgatg attcaccttt aaagccactg gcattttacc
    17281 aagaaggcac agacgttgtt tttccattta cctttgatta tgaaggatta aatgaagccg
    17341 ctcagtcgta tacattcagt atcacgtttg aagttgcttt ctttcaatca ggcggtactg
    17401 agccaaataa tgactttcaa gtgagtctta cttatgctga gcgagcagca aacacggaat
    17461 tttcaattac gcctattcct tcatcaagac ccattttttc gaaattatac tatggctcta
    17521 gagaaatcga cagccaagga aatcttggga atctgctgat tgacgggcaa gacgtgacca
    17581 acagtaataa atttattttg caggtaaatt atgaaggatc acgaaaagac ggtgtagtgg
    17641 ttaccgatgt actaccgcaa ggaacaactt tggcaccgaa tccgactaca tggagttcca
    17701 ctggaaatag agaacctatt tcaggtgggg ttcggatcgc agaagtaacg aatccaggtg
    17761 aagcaaacgt tagaactcat tatgtttccg aggaatttgt ggacaaagta tcttttaata
    17821 aggaaacgaa tacacttgtt gtggactttg gtaatctagc agagggtcaa tcgtatttta
    17881 ttgaatatgc tcttgaagtg accgatgtcg acttgttcaa tgaaggaaga caagaggatg
    17941 ggttttctcg gaatcgagcg agtatgacct acaataatca ggtaatgagc cgagactacc
    18001 gcttgagaat ccctgaaagt aatcttccgg caatctcttt aagaaaaagt gtggacaaaa
    18061 atgttatcaa tctcaatgaa aatgaactaa cgtatactct gcatgtctcg atgttttctg
    18121 gaacaagaaa tacgcttgtt attgaagatg ttttggaaga aggcatggaa tttgtgtcat
    18181 ttctatcaga tattccctct ttcattgatg ccgaagtcaa taatggaata gttcgtttca
    18241 gtgcgaataa tacgattggt ggtccgacaa gttttaccct ctcttttaag gtcaatgtag
    18301 aggcgtatgc ggtcggcgat caaattttaa actttgcaac gcttgtgaat agtgacaatc
    18361 gattgaatac gagtactgta agaacaagga aaatcgatgg gcgtattaaa gtaattaaaa
    18421 ctgatgagaa tggccaaagg ctggcaggag caaaatttga aattttatca agcgattcac
    18481 aagtgataca agaaggtata acggcagaaa acggagaatt tttaagtgaa ccgcttcctg
    18541 taggtagcta tcaagtccga gaaacagaag caccggaagg gtatctttta gatgcgacgg
    18601 tacatgatgt ttttataaat gaatactcca tagaagaagt gacgttaacc attgaaaaca
    18661 tccttgatac gggtggtgtt gaactaatta aacatgctga aggcagtggt gaagtccttc
    18721 aaggagctgt ttttgagtta cagaaccgag aaggagaaac gctacaaacc ggt
//
LOCUS       DAFBQH010000051        18756 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.053, whole genome shotgun sequence.
ACCESSION   DAFBQH010000051 DAFBQH010000000
VERSION     DAFBQH010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.053"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..2226
                     /locus_tag="LK694_001868"
     CDS             <1..2226
                     /locus_tag="LK694_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cna B-type domain-containing protein"
                     /protein_id="HBK5052716.1"
                     /translation="SVPNGHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQ
                     DRPGNTSLISRIYDAPDQNNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPA
                     AGLSKAEVESRMRQMVSSDEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDP
                     IAEPFIYQPGTLSVKSVGTSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQT
                     ENEDFHPNFWYQMNGRTTFQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLAD
                     RPDQVTFEIQREHTTNAAAWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEIL
                     SLPQYNNQGQAFSYQTIKELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKD
                     LIGAVFKLTGDSIDTLLTDHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTW
                     EIKIASDGTVTIDGKTVTTSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAF
                     SLQKKEANGTYQPIDSQTTNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQID
                     KYGKIIYTGKNTEMTNNVWTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDL
                     ESPKDGQETDTFLFENLKPGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHE
                     SVLSPGAKNNQISLDITNQAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGG
                     S"
     gene            2229..3650
                     /locus_tag="LK694_001869"
     CDS             2229..3650
                     /locus_tag="LK694_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBK5052717.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            3647..5524
                     /locus_tag="LK694_001870"
     CDS             3647..5524
                     /locus_tag="LK694_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBK5052718.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            5616..6458
                     /locus_tag="LK694_001871"
     CDS             5616..6458
                     /locus_tag="LK694_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK5052719.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(6610..7485)
                     /locus_tag="LK694_001872"
     CDS             complement(6610..7485)
                     /locus_tag="LK694_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK5052720.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            7692..8723
                     /locus_tag="LK694_001873"
     CDS             7692..8723
                     /locus_tag="LK694_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK5052721.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            8812..9117
                     /locus_tag="LK694_001874"
     CDS             8812..9117
                     /locus_tag="LK694_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBK5052722.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            9296..11311
                     /locus_tag="LK694_001875"
     CDS             9296..11311
                     /locus_tag="LK694_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBK5052723.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            11313..11597
                     /locus_tag="LK694_001876"
     CDS             11313..11597
                     /locus_tag="LK694_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5052724.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            11623..12996
                     /locus_tag="LK694_001877"
     CDS             11623..12996
                     /locus_tag="LK694_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBK5052725.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(13079..15094)
                     /locus_tag="LK694_001878"
     CDS             complement(13079..15094)
                     /locus_tag="LK694_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBK5052726.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(15116..16330)
                     /locus_tag="LK694_001879"
     CDS             complement(15116..16330)
                     /locus_tag="LK694_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5052727.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            16551..17495
                     /locus_tag="LK694_001880"
     CDS             16551..17495
                     /locus_tag="LK694_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5052728.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            17791..18735
                     /locus_tag="LK694_001881"
     CDS             17791..18735
                     /locus_tag="LK694_001881"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBK5052729.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         6346 a         3470 c         3570 g         5370 t
ORIGIN      
        1 tccgttccaa atggacataa aaaagtgatc gttttgctga cggatggtgt accaacattt
       61 tcctataaag tacagcgggt acacgcacaa tcaagcagca attattacgg aactcagttt
      121 tctaatacgc aagatcggcc gggaaatact tctctaatct caagaatcta tgatgcacct
      181 gaccaaaaca atctatccag aagaatcgac agtacgttta tcgcaaccat cggagaagcg
      241 atggcactca aagaacgagg aatcgaaata catggtcttg gcatccaact tcaaagcgat
      301 ccggcagctg gtctctcaaa agcagaagta gagtctcgta tgcgacaaat ggtttcatca
      361 gatgaaaaag gcgatcttta ctatgaatca gctgatcatg caacagatat ctctgaatac
      421 ctagccaaaa aagctgtaca gatctcagca actgtaagca atggacaaat aaatgatcca
      481 atcgcagaac cattcattta tcagcctggt acactttcag tcaagagtgt ggggacaagt
      541 cctacaacgg tcactccatc tatttccata gaaggaaata ccatcaagag caatcagatc
      601 tatttaggaa aagaccaaga aatccaaatc cattaccaag tgagaatcca aacagaaaat
      661 gaggacttcc atccaaattt ctggtatcaa atgaacggca ggacaacttt ccagccaaac
      721 attgatacca atgaattagc tgaattcggt ataccatctg ctaaagctcc cggagtcagt
      781 cttcacatca aaaagttatg ggaagaattt gacaacaatc tagctgatcg tccagatcaa
      841 gttacttttg agattcaacg ggaacatacg acaaatgctg cagcttggaa aaacggatat
      901 attcgaatca ttaaaccagc taaagataca acaaatacgt gggaacgtgc agacattgac
      961 aaattatctg caaatagcgg agaaagttat caagagatat tatcactacc tcaatacaat
     1021 aatcaaggtc aagcattcag ttaccaaaca atcaaagaat tacctgtacc aggatacgat
     1081 tctcaacaaa tagatgcaat gacatggaaa aatactaaac aattcacacc gttaaacttg
     1141 aaaataacga aaaattcctc tacaggtgaa aaggatctta ttggcgctgt tttcaaatta
     1201 acaggagatt ctattgatac tttactaaca gatcatggcg acggaaccta ttctcttcca
     1261 gaaaatgtca aattgcaaaa agaaatgacc tatacgctga cagaaacaaa agctccagaa
     1321 gggcatggat taagcaaaaa gactacttgg gaaatcaaga tcgcttctga tggtacggta
     1381 accattgatg gaaaaacagt cactacttcc gatgatacga tccagttgac tattgaaaat
     1441 ccttttgttg aagttcctgt agcagtacgt aagtatgcga tgcaagggac ggacaaagag
     1501 ataaatctta aaggagcagc attttcccta cagaaaaaag aagcaaatgg tacttatcag
     1561 ccaattgaca gccaaacaac gaatgaaaaa ggtcttgcca gttttgattc actcacacct
     1621 ggtaaatatc gagtcgttga aacagctggt cctgccggat atgatacttc gccgggaaat
     1681 tatgaattcc aaatcgataa atatggaaaa atcatttaca cgggaaaaaa taccgagatg
     1741 acaaataatg tatggacgct cactcatcaa aatcgactaa aagcgtttga tctaacggta
     1801 cacaaaaaag aagacaacgg acagacatta aaaggagcaa aattcagact gcagggacca
     1861 gaaatggact tagaatcgcc aaaagatgga caagaaacag atacctttct attcgaaaat
     1921 ttaaaacctg gaacttatac gctgaccgaa acttttacac cagaaggata ccaaggtcta
     1981 aaagagccag ttactatagt tatacacgaa gatgggtcaa ttcaagtgga tggacaagat
     2041 catgaatctg ttctgtcacc aggagccaaa aacaaccaga tttctttaga catcacgaat
     2101 caggcaaaag taccattacc tgaaacggga ggaattggcc gtttaggaat ctatctagta
     2161 gggatgattg gttgtgcgtt ttctatttgg tatctttttt tgaaaaaaga aagagggggc
     2221 agctaaatat gaaaaaactt ggttggctta gtatgtgtct cttcttgtta ctatttaaac
     2281 cagcttttac tcaggtagca acagaaacag aaacagaaat ggttcagatt actttacaca
     2341 aattgctttt cccaaacggg caactgccga aaaatcatcc aaatgacgga caagaaaaag
     2401 ctttattaca aacgtatcga ggattaaatg gtgtcacatt ccaagtttat gatgtcacag
     2461 attcttttta ccatctacgg gaaaagggca aaacggtaga agaagcacaa gcagagatcg
     2521 caaaaaacgg tgcgtcttcc ggtatgttta ccgcagaagc aacaactaca actcttaaca
     2581 acgaagatgg tatcgcttct ttttctctgg ccgctaaaga tcaagaaaaa agagataaag
     2641 cgtatctttt cattgaatcc aaagtaccag aagtcgtcaa agaaaaggca gagaatatgg
     2701 tagttgttct tcctgtacat ggacaaaaca atcaaaaact ttcaactatc catttgtatc
     2761 ctaaaaatga agaaaacgac taccctgatc caccttttga gaaggtatta gaagagccta
     2821 gaaatgattt tacgattggt gaaaaaatca cttattcctt gcatacgaca attcctgtaa
     2881 atatccttga ctatcaaaag ttcgaattgt cagatagtgc ggatgaagca ttaacgtttt
     2941 tacctaatag tttaacgatt tcatcgaatg gagaaaagct gacagaaggc tttgtcatac
     3001 acaagaaacc tcacggattt gatgttttat tttcgatccc ttcgttggaa aaatatgctg
     3061 gaaaaaaact gaccatttct tatcagatgc agctaagcag tacagcacag gcgaacaagg
     3121 aaatcaacaa caacggaaca ctggattttg gttttggtgt cagtacaaag aaagtctctg
     3181 tatatacagg gagtaagcaa tttgtcaaaa tcgagacaaa taaaccagat aaacgattag
     3241 ctggcgcagt attccttatt aaaaacaaag caggaaatta cctccagcaa acagccaacg
     3301 gatacaagtg gacaaagaac gaatcagatg cgcttcacct gatttccgat aaaaatggcg
     3361 ctttttcaat ttccgggttg aaaacaggaa gttatcgatt aaaagagatc gaagcacctt
     3421 ctggttatat tttaagtgaa acagaaattc cgtttaccat ttcaactttt ctttctgagg
     3481 ataaagaggc ggacagtata ttgaaagtag tcaataaaaa agaaaatagc cgtccatttc
     3541 ttccaaaaac aaacgaaacg aaaaatacac ttttaggcgt tgttggtatg gtattcgcaa
     3601 gctttgcaat ctggttgttt atcaaaaaaa gaacaggagt gaaaaaatga aaaatcataa
     3661 aaaaataaac gttatgttag gagtcctttt ccttatttta ccattactca caaacagctt
     3721 cggcgcaaaa aaagtgtttg cagaggagac agcagctcaa gtcatccttc ataaaaagaa
     3781 aatgactgat ttacccgatc ctttaatcca aaacagcggg aaagaaatga gcgaattcga
     3841 tcaataccaa ggattagccg atatttcatt ttcagtttat aacgtcactc aagaatttta
     3901 tgcgcaacga gataaaggag cgtccgtgga tgcagcaaaa caagcagtcc agtctttgac
     3961 tcctggtaca ccagttgctt caggaacgac agatgctgat ggaaatgtca ctttatcttt
     4021 acctaaaaaa caaaatggga aagatgcagt ctacacgatc aaagaagaac caaaagacgg
     4081 agtgtcagct gccgcaaaca tggttttagc tttccctgta tatgagatga tcaaacaagc
     4141 agatggctct tataaatacg ggacagaaga actagatact atccatctct accctaaaaa
     4201 tacagtcggt aatgatggaa cgttgaaagt tacaaaaatc ggtactgccg aaaacgaagc
     4261 actaaatgga gcagaattta ttatttctaa agaagaagga acaccaagcg tcaaaaaata
     4321 catccaaagt gtcacagatg gattgtacac ttggacaact gatcaaacca aagccaaaca
     4381 tttcattact ggtcattctt atgacatcgg caacaatgac tttgccgagg catctattga
     4441 aaaaggccag ttgatcgtta atcatttaga agttggaaaa tataatttag aagaagtaaa
     4501 agctcctgat aatgcggaaa tgattgaaaa gcaaacaatc acgccttttg agatcctggc
     4561 aaatagccaa acaccagtag aaaagaccat caaaaatgat acgtctaaag ttgataaaac
     4621 aacacctcaa ttgaatggaa aagatgtcgc aatcggtgaa aaaattcaat atgagatttc
     4681 tgtcaatatc ccattaggta tcgctgataa agaaggaacg caaaacaagt acacaacatt
     4741 caaacttatc gatactcatg acgctgcttt aacatttgat aatgattctt caggaacgta
     4801 tgcttatgcc ttatatgatg gaaataaaga aatcgaccca gtaaattatt ctgtcactga
     4861 gcaaacagac ggattcacgg tttcagttga tccgaattat attccttcat taactcctgg
     4921 cggtacattg aaattcgttt actatatgca tttgaacgaa aaagcagatc caaccaaagg
     4981 attttctaac caagcaaatg tcgataacgg gcatacaaat gatcaaacac caccgtcagt
     5041 cgatgtcgtt actgggggca aacgatttgt taaagtagat ggtgacgtta catcagacca
     5101 aacacttgct ggagcagaat tcgtcgttcg tgatcaagat agtgacacag cgaaatattt
     5161 atcgatcgac ccatccacaa aagccgtcag ctgggtatcg gcgaaagaat cagcaacggt
     5221 ttttacaacc acaagtaacg gtttaatcga tgtgacaggt ctaaaatatg gcacgtacta
     5281 tctggaagaa acgaaagcgc cagaaaaata tgttccatta acaaaccgtg tagcatttac
     5341 tatcgatgaa caatcttatg taacagcagg acagttgatt tctcctgaaa aaataccaaa
     5401 taaacacaaa ggtacacttc cttcaacagg cggtaaggga atctatgtgt atatcggtgc
     5461 aggagtagtc cttctactga ttgctggact gtactttgct agacgcaagc acagtcagat
     5521 ttagtctttt tgcaagaatt ggaaaaagga gcagcggatt acgcaaatgc tcctttttct
     5581 tttattatca aacagtcatc acaaggagaa cacaaatgaa accaaagaaa aggaaacttc
     5641 tagatctgct gatgcttttc ttgcttctct caggaatagg gatactagct tatccatttg
     5701 ttagcgacgc gctgaataat tatctggatc agcagatcat ctctcattat cagcaacaag
     5761 cggtcaaaga aaatgaagaa gtcatggcta aaatccaaga aaacatgaca aagaaaaacc
     5821 agcaattagc aaaagaaggt ggaaatccag gtgcagatcc ctttactaag aaaaaagaac
     5881 ctgcgaaaaa ggatagaacc tattttgaga aacatacgat cggtattttg acgatccctg
     5941 agatcaatgt ccatttgcca atttttgatg aaacgaatac tgtgctgcta gaaaaaggtg
     6001 cctcattatt ggaaggaacg tcttatccta cggggggaga aaatacacat gccgttattt
     6061 ccagccatcg aggactgcct caagcaaagt tgttcacaga cttaccgaaa ctgactttag
     6121 gtgattcttt ctatatcgaa atcaatggac ggacactcgc ttatcaagtc gatcagatca
     6181 agacggtaga accgactgat acaaaagatc tgagaatcga aaaagggcag gatttcgtga
     6241 cactcctaac ttgtacacct tatatggtca acagtcatcg cttacttgtc agagggcacc
     6301 gggttcctta tcatgcaaaa gaagttcaaa aagaagtaca gaaagtatcc gagaacaaac
     6361 gatttttcct gtatggattg ttagctgcat gtatactgat tttcctcctt cttatatatc
     6421 tttttcgtca atcgttcaaa ataagaaaca gaagttagct attcctcatt tctataatat
     6481 atctattcga atcgttccgt aaacaaacag gctgaggttt gaaaaagaaa acttcagcct
     6541 gtttttatga tgatatcaaa gagagcctat caaacactat ctttcttatt actaaatgag
     6601 gatcttttct taagttttcc gatattcttt aagaaggcct ttttgcatca attcttgttt
     6661 gtttttcaag ttgcggatat tctcttcaaa attttgagaa tatatcactg agaataacgt
     6721 atttaatacg taatcgaagg caatttgaga ggagaaagtc gctactttag caaaatcata
     6781 ttcctcctgc gatataacaa gacacatatc agcctgtttg gccatttctg aatgctgatt
     6841 tccggtaatc agcaaggtag gggcctctac atgcttcaaa taggtcatta ttcgttcata
     6901 atgatggatg cgaccgctgt agctaataaa aatggcgcaa tcctcgctag tcaaattggc
     6961 tgcattccaa gaagaatcac tgtattcttc agccatgatc aaaaacttat tcaacttgac
     7021 cattttattc tggaatttcc ttgccgtgat ttgagaatca ccttttgcaa aaagaaagat
     7081 tcgctgtgcc ttgttcaata ctttaccgat ttgatctaaa tcttcatgtt caatctgcaa
     7141 ttgtgctttt tttattgact ggatcgataa atccgccatt ttctttgcaa tttgctgagt
     7201 gctatcttca cttgaaaaag gaaaattagg atcaatcgtt tgattgaact gagcaatcaa
     7261 ttgatgaact tcccgcccca gctcgaaacg gaattcttta aatccgctga atcccatttt
     7321 tttgcataaa cgaatgatag ctgaatggga agtatacgta gcttttgcta attcttcaat
     7381 cgtcatgtag actgcagctt cgatattcct acggatgtaa tcgacaatcc gtttctctgt
     7441 cgtggtaaac aactcttgtt ttgttagttt ttcttcaata agcatgatct gacctccaaa
     7501 ttctatctta ctgataaaca cttggttccg ctactatctt tttgattttt atccttactt
     7561 tgaacattaa tttcaccaaa ccgaatgata acaagtgttt ttctgaactt ttttaccaga
     7621 atagctatta taaatacttt ctgcgctttt ctaggatata ctgaaaacat caataaaagg
     7681 aggcaccact catgcttaca atcgcttatc ttggcaatgg aaagagtaca aatcgctatc
     7741 atttgccttt ttcaagaaaa cttacgaaca aaatcaatat caaaaaaatt ttttccccat
     7801 ctgaaccagt gtggaaaaca ttcgaagaca tcgaatatac tgatcagctg tctgatcttt
     7861 gggatgatcc tgagatccaa ttagttgtca tcactacacc tagtcaattt cattatgaat
     7921 atggaaaaat ggcactcgaa catggtaaga atgtactagt agagaaacct tttgcagaaa
     7981 cgtcaaagga agcagaagag ttatttgcat tagcaaaaga aaaaggtcta tttatccaat
     8041 gttaccaaaa cagacggttc gattctgact tcttgacagt ccaaaaagta atcgaaagcg
     8101 gaaaattagg ggatatttta gaagtagaaa tgcattatga ttatttcaga ccggaagtac
     8161 cagaaaatac gcatgaattt tctactgcga acagctattt atatggacat gcttgtcata
     8221 cagtagatca agttctttct tatttcggcg aaccagatga tatcagctat gatgtacgtc
     8281 aacttttagg gaaaggacga atgaatgatt acttcgatct ggattttttc tatggaaata
     8341 tgaaaatttc gataaaatcc agttatttcc gcatcaaaga acgtccaagt ttcgtcgttt
     8401 acggaaaaaa agggatgttc gtgaaacaaa caaaagatcg acaagaagaa catctgaaaa
     8461 tgttttacat gcctgatcat gaagactttg gtatcgattt gcccgaacat tatggtgtat
     8521 tgacctatat agatgaacaa gggagttatc atgaagaaaa agtaatttcc gaggttggtg
     8581 attacagccg tgtctacgaa gggatttacg agacattagt taatggcgca gaaaaagtca
     8641 tcaaagatga agaaacaatc ttacaaatga aaattttgga agaaggaatc aaaagcattc
     8701 aagaatcaga aggaaaaata tgacatattt gtaagaatcg ggtttgtttt ttgacgcata
     8761 aactgtctta ttgtacgatg aaaaagtata gaactactag gaggaaccaa catgacaaat
     8821 tatgacggtg aaaaacacaa acgattaaga ggttcaatgg cccctaaaac agaagaagaa
     8881 gtggctttag caggaactaa ttctggtgaa acagaagaca aagaagaatc attagctggc
     8941 acaaactctg gtgaaacaga aaataaagaa acagctttat ctggttcaaa ttctgtagaa
     9001 gaggaaacaa agcctaatga gcccaatcac tttgaaaaag atatcgaaaa agcggttgaa
     9061 gatgtcaaag aaaaatttga agaactgacc tcaaaaaata aacatgatca tcattgatcg
     9121 gacgaaatca attcataaaa gcaggagaca tttggtcacc tgcttttttt ctgtatccat
     9181 tcgatagaaa tcgttcggta cagtcgtaaa aaaaatccaa ctgtaagttg taccaaacac
     9241 ccctcgattt aaaagcgcta ccatactaga atataagtag gaggtgaaca gcgctgtgaa
     9301 ctacaaaatt agttatctcc tgaaaacacc aagagaaatc agtctagaaa aagcagcaga
     9361 gatcatcgaa gtaaatacaa gcgaggcgat caaacaatta actccttttg ccgaatatgt
     9421 ggatgatacc cgtatccgta taaagaaatt ccgagcaatc gactgggcag aaaaattttt
     9481 ggatattact cgatttgaag ctgtgtatac cgaacaagag cgaagagatc ttctttattt
     9541 gatggtttat tcgactgcaa cgtctgtttc tgtttttcat tttcaagatt ttcttcaggt
     9601 ttctaaaggg acagtcctgt cagatatcaa aaagataaga aatgaattag aagtggaaga
     9661 ttgtcagctg tcgtatacta ggaaaactgg ttttaggctg ctagggtcag aagcaatatt
     9721 aagaagagaa gcaaaaaatc gcactgctcg tttgatgcag tcacttgctg gaagatttgg
     9781 gctcacgtat ttaaccgcaa ccctttccat tgattattac gccaaagtta gagatttgct
     9841 agataaaacg atttcactat cagatcttcg attcgcacca aatcgaatgg atgaattagc
     9901 ttattatttg agtttgacaa ccattcgaat tggaaaaaca tccatggaac tggtaaaaga
     9961 tgctgaactg ttggcttctc ttgcagtcac tgacattagt cgacaagtat tgactggcct
    10021 tggctattcc gaacatcaaa attcggagat tatatttttc tctctttgct tagcatcgat
    10081 cgttgaagga gatgtccagg agcctgcact tgattttttg ctggaatgtt cttcagaagt
    10141 catccatcgc atggaattcc tgatggccgt agaatttgct tcgtttcgtg aattattgat
    10201 gaatgtcttt tatcacttgg tccctgccta ttttcgtatt tcctattctt tttatttacc
    10261 taatgtgatg atcgatcaaa ttaagcatca atatgcttcc atctacgaaa tgacaagaaa
    10321 agcattactt ccgttagaaa gacgaatagg gaagagcatc cctgaagaag agattggttt
    10381 tttcactata ctatttggcg gagagatcag aaaagcggac gcagaagaaa gaaatagaaa
    10441 aataagagcc gtgatcgtct gtccaagtgg cattagctct tcattgatct taaaaagtga
    10501 acttcagcaa ttattcccga tgatcttttt tacagagact aattcagctg accgcttaca
    10561 cgaggttgac gaaacttctt acgatattat tttttctact gtacctctca cacttactga
    10621 taagaaaaag tctctctatg ttctgcatcc aatcatgact gacttggaaa aaaatcagct
    10681 gatcaatcaa gtacaaagag attggctgat accaggattt tcaatgcctt ctgcccaaga
    10741 attgttgtcg gcgctactgc cttatatcga attaaaagaa ggcgtagacg aagaaaagct
    10801 ctatcaagta ttaaatcgta aaatgaacaa acttttagaa aagaaggagg atcaacgtcc
    10861 tatgctatct gaattattaa cagaagaaat gatccaatta tcggatcaac caaaaaactg
    10921 gcaagaagca atcacatttg ccgcacagcc gctattatca gaaaataaaa tagaagaaag
    10981 ttatatcaaa gctatgatcg agcgcgtgga gcagtatggt gcgtttatcc atattggcga
    11041 tcacatcgca ttacctcatg ctcgtccaga agatggcgta aacgaagtag ggatgtcttt
    11101 gctgaaactt caacaacctg tggatctgct tgataatcca gcccatccca ttagtttgtt
    11161 catctgttta gctgctatcg ataatgaggc acatcttcgg gctttggcaa atttaacaaa
    11221 gctactttct aataaacaaa atcttcaaga tttattacac gcatcaacaa aagaagaaat
    11281 attagcgatc ataagaaaag gagacgaatg atatgaaaat ttttgcagta tgtcaaagtg
    11341 gattaggaac aagttttatg gtagagatga acatcaaaca agcacttgca gatcttggag
    11401 cagatgtgga cgagtttgaa gtaagtcata cggatgttgg aagtgtatct agcgatatgg
    11461 ctgattattt cttcattgag cattctttga aagatacgct aacttctata cctgatgaaa
    11521 agctcgtacc tttgcaatcg atcattgaca gcgaagaagt aaaagaaaaa gtaacgaaga
    11581 tcttgtcaaa agaataaaaa tgattcaagg gaggaactat caatggataa attgttgaat
    11641 ttgtttgtgt caatcgtcag tcagccctcc atcctcgttt ctttgattgc actgcttggt
    11701 ctcgttcttc agaagaagaa attttcagaa gtgatccaag gaactatcaa aacatttgtc
    11761 ggattcctag tactgatcgg cggtgctggt ttaatcagta attcactaac cccttttgct
    11821 tcgatgttcc aaacagctct gcatactcaa ggagtagttc ctagcaatga agcagtagtg
    11881 gcgattgctt tgcaacaata cggtacacct actgctttga tcatgctagt agggatgatc
    11941 gtgaatattt tacttgctag attcacaaga tataaattca tttttcttac aggtcaagca
    12001 atgctttatg tttcttgtct aacagcagtc attttagtaa gcgcggggtt tactgcaaat
    12061 ttgtggatga tcttattagg cggggtcttc gaaggaacac tattaactat tacgcctgct
    12121 ctttgtcaac cgtttatgaa gaacatcact ggaaacaatc aagttgcgtt agggcatacc
    12181 ggaaatatcg gttatgcttc tagtggatgg atcggaaagt tttttggaaa caaagaccgt
    12241 tctacagagg acataaagat tcctaaaagt ttcagctttt tgcgagattc tactgtatcg
    12301 atcatgctct tgatgtcgat cgtatatgtc gcacttgctt tgttagctgg tcctggattt
    12361 gttgaaagag aactaagcaa tggagacaac gctgtaatct ttgcactgat ccaagctgga
    12421 acattcacag ctgggtttgt tatcgtcctt caaggggttc gaatggtgtt ggcagaaatc
    12481 gttccagcat tccaagggat tgctaaaaaa ctcgtacccg attctaaacc agcattagat
    12541 gttcctatcg tttttccata tgcacctaat gctgtattaa ttggcttttt tgttagcttc
    12601 tttgtaggaa cactatccat gtttgctatg ataggcttgc aaactacagt tatcattcct
    12661 ggggtagtag gacatttctt ctgcggagct gcttctggtg tcttcggcaa ctcgactggc
    12721 ggtagaagag gggcggtatt aggttctgct ttcaatagct tattgattag ttggctgcca
    12781 ttatttatcc ttccagtatt aggagatctt aaaatcgctt cttctacatt tgccgatact
    12841 gattatctag taccaggact tgtacttggg aaactaggag aatacggtca aatagccttg
    12901 acagttggaa tcatcgcttt ctttttactt gtcgtattat ctagctttct ttttgccgga
    12961 aaaagaacaa acattgtttc taaagaacag aattaagcaa acttcaaaat aaaaattgct
    13021 tggacaatat cagaagaaga tttaaagtct tctgatatgt cgcaagcagt tttttgtttt
    13081 atgcatttat ttttgaatag cttgttaaga caagtggctg ttttgctaga agaggataac
    13141 ttttaccatt cattttcact gttccgcctg ttctatcaga agtaatcacg atcttatctt
    13201 ttgttttttc aagcgaaatc tttccatctt tgataggtag cgtacaattc agtgtttcaa
    13261 atgaatctag attaggttgt atcataaatt cttcataacc tggctttgtt ggatacaaac
    13321 cgataaaata gcgtcctaaa agatagattg ggcttgctcc ccatgcatgg cataagcttt
    13381 ttccataagg atctccatac atactgtact tttcatttcc tgtttgagta gggtcgtatt
    13441 cttcccagaa agttaccgca tcatgagata acatccctcc ccaataatca agaatcgttt
    13501 ggtagacgat attcgtctgt ccgagtttgc ataaagcatc ttgttcatag aatttgaaat
    13561 atggagtagt aatctgtgtg atagtgtcat tcaataagac attctttaag atcgattgct
    13621 gttggtcttt gttagcaaaa tcgaataaaa cagcgaaaat attcgcatga cgcgtcacat
    13681 ttcttctccc agattcatag caatcgataa aagcgccaag ttcttcatcc caaaagtagt
    13741 caaataggtt ttgtttcaat agttgatatt tttcttgata gagatgagta tgataatttt
    13801 cgttctctcc atatgtttca gctaagatat ctccgcaagt gatcatggaa tgataggctt
    13861 tcagtaataa gacttgttct gcagcaagcg ttccttcttt atccatttca gaccaatcaa
    13921 taaatatcca atcttttgct ctcccataaa taaaccctaa ttcattcgtt tgctccatca
    13981 agtatctcat taatgattgc atcttagggt agacgatgcg taaaaattca tagtctttcg
    14041 tcatcatata gtggttttcg ataccaatca accacaagat cgaatagtca acgatagtat
    14101 tcatatgctg cttgatttca ttttgtcctc ttaatgctaa tatcgttcgt ttattgattt
    14161 cctcgtcaaa aaaaagatat tgattgatca tgaatgattg atacgcatct ccagaccaaa
    14221 tccagcgatc tcgtttgata ccgtcgataa aaaacaaatc actacataac ttgaaggctt
    14281 cttctgatac tgaccagatc tgattcagta aagaagaatc gcttgaaaaa gcagcttgtt
    14341 tcgaaagaga taaaaattca tgaaaaactt gaagttgaac cattccagta gaaacctctg
    14401 gtataaacaa atagcgaaaa gcgcgtttac gagctttcat ttttcttgtc attcctattt
    14461 gcttatagta gcaataatcg atatccatag cttctacatc ggactctcca taacatagag
    14521 tgatagattc gatatggtca tccagagata ttagttctac acagccattc actgctcgcc
    14581 caaaatcgaa taagattcct ccgccaatct ccgtttcagt gactggatgg acgcattctt
    14641 tttgataata gatttgattt ggatcttttg cgatatcgac ataaagcgga ctccagccag
    14701 ctggatattc ttcaataaaa ttacttgctg tccagccaat atctgatttt acttcgtctc
    14761 cctcgataaa catcgctggt agccctgatg tatgtcctac aaagatacga atttttgctt
    14821 taccagctgg acaagtcagc cattttccac atggatgttt ttgtccatta atttctacat
    14881 acccaactcc ttggatcgtc actttaaaca tggaaggttg atcaagaaag taatatctct
    14941 taaacttgac gttccgatgg caatcatcca tgtaccagta cgctggccag tcaaaacctc
    15001 gttcttcccg ttggaaattt tgtagcagtc catggcggat ctcaaagtct ccaggatacc
    15061 aaagccatct atctttatgt ttttcatttg tcattttaca cgacctccat caaatttatt
    15121 ttgttgtcaa ttgttctttt atttcttcat ctactcttat cactgtcttt ctattcgtcc
    15181 tgtgtactac tagaaggcta ataagcgcgc taatcaacag caagatactg ccattgacga
    15241 aaacagcaag tgttttactc ccaagagaag caaaagagag aaagagcaaa ccatatggtg
    15301 caatgaaagt acctaaatta gcgccaacca ataagattga agtattgaaa ctctccgcgt
    15361 acttgggcgc ttggatactt acttggttga ataagtaagg aatgaataac gaaaaagaaa
    15421 ttcctgttag gatcgcacca aacccaatca catacaggtt tatcacagtg gcaatcaata
    15481 caaagccgat tcccatcagt agtaaagaaa gtgtaagcat atagcgatgt gcttttgtct
    15541 gaagccagcc aaaacaaact ccggcagcca tacttgccaa ttgcatgatt gttaagatat
    15601 tcgctgcatt agtttcgttt cctagagaat aagtcgtcag gactgtggct aatttaatcg
    15661 atacagcatt atataccacg atgaccagca agagaaaaat accataacta atcattttag
    15721 gattcgtttt gattacttgt cttgttgggg ctgtgccatg cgattgaagt tcagttgttt
    15781 caggtgtagg aacaaacaaa acaaacaaga caagaatagg taagctgatg gcgtagacta
    15841 aaaaggtata ttgccaaccg aactgtacta gctgtcctgc aacaaatgtc attaaagctg
    15901 caccaaatcc ttgaaatgca ctttgaaaac caatcataga ggctcgttca tctccgtgat
    15961 aaaaggcagc aatcatgctg actgccaaag aattgatcaa tccaaagcca atacctagtc
    16021 cagctcttga agctaagaca atactgtaac tttgtgagaa gagggggagg ataccacaaa
    16081 tcgatgcaat cactaatccg atgatcacag tttgtttttg tccgattttc ctagcaataa
    16141 aactactaat caaaacaaaa atagtgacca taaaagaggg agcagtcgtt aataattcaa
    16201 cagcagataa agaacgcgtg gcataagtag ctgacatact gggaattgca gacgtgactg
    16261 caccagcaga agtcaaaata agagagactg acagcagaga aaatttcatc atctttttat
    16321 tcataagcat gttcctttca tttttgtaag cgcttatcaa aaatatagta tgaagtggtt
    16381 attctctcaa tagcggaatc acaggaagtt catgtcccaa ccatagaaag tctttatgaa
    16441 accaaaaata gctttgcata taatagcagt acagcgtgaa ctaataagcg aaagaaaccg
    16501 cttacaaaaa ggagggagtc aattggaact agctgaatta gacatttttt ttgcgtaacg
    16561 aagaatcaat cgaaactctg cagcgtgaac aaggaatgaa tatcaatgat ttaaacttag
    16621 cttaccatct tcagacaccg ccaaaaatcc accgagggga tctatttcaa cagggaaata
    16681 ttcatatcag taaacaccgt cgatatgcgg aagttcccga gcatacacat gattttgttg
    16741 aaatcaatta tatgtatagc ggtacatgtt tacaaattat taatggagaa aggatagaga
    16801 tggaagaaag tgacgtggtg atcattgata aagaagctac tcaaagaatc gaatacacag
    16861 ggcaagagga tattttaatc aacatcttat taaaagaaga aaccttatct acagagatat
    16921 tgaatcattt ggcaagcaca aataatctac tttcttcttt tttgataaat gcatcaaaag
    16981 aaaaagggga acatacacaa ttcattcatt ttcgcggcgg aaaaacaagt cgtttgacgc
    17041 atttgattga aaatatgctg ttgatttatt ttgatacata cagtcataag atccgttcgt
    17101 taaatctcta tacatcacta ttactgatcg aattgactca tttattagaa caacaaagct
    17161 tttcatcagc agatggatgc gcagaaaatg agatgcttag agtattagat tatatcgata
    17221 agcattttac ttcttgtacg ttaaaagaaa cagcagctca ttttggctac aatccggtct
    17281 atcttagtaa taaattgaaa atgtattttg atgcttcctt tcaagaactg gttctaaaga
    17341 aaaaattcga ctttgcgttg gaactgattc aagaaacgag tttttcgttg gaagaaatcg
    17401 ctaatcgtat cggatataaa caaacgactt ctttattcaa attattcaag aagtattcaa
    17461 catgtactcc caatgaatgg agaaataaac attaagaaga aaatctttca ctaaagcata
    17521 tgaaggaaaa ttgttttagg tgcgattgct gtcttattat atcgttgcag cgaatttttt
    17581 tggatttatt ttcagttcta aacttttggt tgtattatca ggggatatag gtgtaaacat
    17641 ttgtcattta tctgttatgc gtattcagga attcgtttcc atcaaaaatt ataataacaa
    17701 ctattataat taacatgttg ataaaatgaa taaacaagac tatacttttc atgaaagtaa
    17761 caaatagtta tgaaaaggag ctgtttattc atgaaaaaaa caagtcgtaa agtagtaatc
    17821 gtcggtacag gttttgttgg aacaagtatc gcttatgcaa tgatcaacca aggaatctct
    17881 aatgaattag tattgatcga tgtgaatcaa gaaaaagcag aaggggaagc attggacctt
    17941 ttagatggta tggcttgggg cgatgaaaat gtggctgtat ggtcaggtgg ctatgaagaa
    18001 tgtaaagatg ctgatatcgt tgttatcaca gctggtatca accaaaaacc tggtcaatct
    18061 cgtttagatt tagttaaaac aaatgcgtca atcatgcgcc aaatcgtaaa agaaatcatg
    18121 ggatcaggat tcgacggtat tatcgttgtt gcttcaaacc cagtagatat tttgacatat
    18181 atcgcttgga acgaatctgg tctgccaaca tcacgtgtta tcggtactgg aacaacattg
    18241 gatacaactc gtttccgtaa agaaatcgca ttgaaattaa aagttgaccc acgcagcgtc
    18301 cacggctata tcttaggtga acatggtgat tcagaagttg ctgcatggtc acatacaact
    18361 gtcggtggta aaccagtgtt tgaaattgta gaaaaagacc atcgtattgc ccaagatgaa
    18421 ttagatgtga ttgctgataa agtccgtaat gctgcttatg agatcattga tcgtaaaaaa
    18481 gcaacttact atggtattgg tatgagtact gcacgtatcg taaaagctat cttgaataac
    18541 gaacaagctg tattgcctgt atctgcttat ttaactggtg aatacgacga aaaagatatc
    18601 tttacaggtg tgccatcaat cgttgacgaa aatggtgttc gtgaagttgt tgaattatca
    18661 atcaacgaag aagaaaaagc aatgttcagc aaatcaacaa gtgctttaag agaagtattg
    18721 aacactgtat tataagaaac aatgaatatg aactaa
//
LOCUS       DAFBQH010000052        18101 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.054, whole genome shotgun sequence.
ACCESSION   DAFBQH010000052 DAFBQH010000000
VERSION     DAFBQH010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.054"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(487..801)
                     /locus_tag="LK694_001882"
     CDS             complement(487..801)
                     /locus_tag="LK694_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBK5052730.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(815..1075)
                     /locus_tag="LK694_001883"
     CDS             complement(815..1075)
                     /locus_tag="LK694_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBK5052731.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            1245..1559
                     /locus_tag="LK694_001884"
     CDS             1245..1559
                     /locus_tag="LK694_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBK5052732.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            1561..3216
                     /locus_tag="LK694_001885"
     CDS             1561..3216
                     /locus_tag="LK694_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK5052733.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            3299..5434
                     /locus_tag="LK694_001886"
     CDS             3299..5434
                     /locus_tag="LK694_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK5052734.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            5609..6118
                     /locus_tag="LK694_001887"
     CDS             5609..6118
                     /locus_tag="LK694_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052735.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            6115..7419
                     /locus_tag="LK694_001888"
     CDS             6115..7419
                     /locus_tag="LK694_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK5052736.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFI
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEEISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            7515..8384
                     /locus_tag="LK694_001889"
     CDS             7515..8384
                     /locus_tag="LK694_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK5052737.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(8496..8816)
                     /locus_tag="LK694_001890"
     CDS             complement(8496..8816)
                     /locus_tag="LK694_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBK5052738.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            8919..9365
                     /gene="dhaM"
                     /locus_tag="LK694_001891"
     CDS             8919..9365
                     /gene="dhaM"
                     /locus_tag="LK694_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBK5052739.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            9340..10350
                     /gene="dhaK"
                     /locus_tag="LK694_001892"
     CDS             9340..10350
                     /gene="dhaK"
                     /locus_tag="LK694_001892"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBK5052740.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            10378..10983
                     /gene="dhaL"
                     /locus_tag="LK694_001893"
     CDS             10378..10983
                     /gene="dhaL"
                     /locus_tag="LK694_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBK5052741.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            11130..11795
                     /locus_tag="LK694_001894"
     CDS             11130..11795
                     /locus_tag="LK694_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HBK5052742.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            12078..12737
                     /locus_tag="LK694_001895"
     CDS             12078..12737
                     /locus_tag="LK694_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBK5052743.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(12814..14424)
                     /locus_tag="LK694_001896"
                     /pseudo
     CDS             complement(12814..14424)
                     /locus_tag="LK694_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
     gene            complement(14437..15318)
                     /locus_tag="LK694_001897"
     CDS             complement(14437..15318)
                     /locus_tag="LK694_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5052744.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            15572..16171
                     /locus_tag="LK694_001898"
     CDS             15572..16171
                     /locus_tag="LK694_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK5052745.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            16658..18046
                     /gene="ltrA"
                     /locus_tag="LK694_001899"
     CDS             16658..18046
                     /gene="ltrA"
                     /locus_tag="LK694_001899"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBK5052746.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT         6137 a         3277 c         3801 g         4886 t
ORIGIN      
        1 aaaaaagggg tgtgacctat gtcgcattcc tttttttgat aaatagactc ccagtagcta
       61 cttctcgata ataagccaac caaaccaaaa acaggagaag ttgtttttga aattatttct
      121 tgtgattcgg agttaaatgg taggctcaca acctcttggt aaacggtgtt caatcagctg
      181 accttcggta ttagctcaaa aaacggcaaa atatgaggag ctattttccc gtttttttct
      241 ctaatactta ggtcaaaccg ctgatttcac aacctcttcc ttaaacggtg ttcaaaagct
      301 tgcatctgcg gtaataagcc caaccaaacg aaaaatatga gaagctattt ttgtttggct
      361 gttcttatta cttgatgcag gacagcgttt tcacaacctt tgattcacgg ttttccagaa
      421 gtagcttctt cgaaaaataa gccgaaattt cttgaagctg atcacttctg tcgcaacctc
      481 tactgactaa ggcttgatat aagcataacc ttgggattgc agttcaacta aatcaaggac
      541 acctgctggt acaacgatta cttgttcagg taaagaactt tctgaaatat gattagcacg
      601 catagcattg gcgcaagcgt gaaaaacgac tccttgcaaa tctaagaatt ctgcattggc
      661 agagtctaga taaccggtaa tcgcgatgcc atttacagat acgacaatcg tcgtttctgg
      721 agaagctttt atcaggtttt ttacgttttt ccctgtttct gaccattttt ctaattcatc
      781 aatatggaaa acgactttca ttgaaatcat tcctttactt tttaataatc atcttgatcg
      841 cttgttctag tttagccgct tgttcttctg gattactatc aggtttctca aagcaatttt
      901 tcagattatc tgcaacgatc atgcccatta cgcgatcaat actagaacga acagcgctta
      961 attgagtgat gacatccata cattcttttt cgtcttcgat cattttttgg ataccacgaa
     1021 tttgtccctc tgtacgtttc aaacggttca ggatattttt attatcaatc tccataacac
     1081 attctcctct atttttataa ttagataata ccccttatcg gtattttatg caaatataaa
     1141 aagtagtgaa aaaaatgaag agtcattctg gttgacatat taataaagct cctgtttaat
     1201 ataccccatg aggtatttat aaaaatgtaa atgaggtgca gaaaatgaat ttgctatttc
     1261 aatcaataaa atcgataaca gtcccagaat taaaggaaaa gcttttggaa aatccagcgt
     1321 tacttgatgt tagaacacct gcagaatacc ggggcgggca tataaaagga gctaaaaatg
     1381 ttccattaca aagtataaac agatatgatg gagacaaaga aaagaccgtc tatgtgattt
     1441 gtcaatcagg gatgaggagt aaacaagcag ctaaagaatt aaagaaatct ggttatgatg
     1501 tagtgaatgt acgtggtggc atgaatcaat ggtttgatcg aacagtagga gggaaataag
     1561 atgaaaatcg ttattgtcgg aggtgtagca ggtggaatgt ctgctgctac acggcttcgc
     1621 cgattaatgg aagatgcaga aatcgttgtt tttgaaaaag gaccgtatgt ttcttttgca
     1681 aattgcggct tgccttatta tctttcggga gaaatcagtg aacgggaaaa tcttcttgtc
     1741 caaacgccag aatcattatc cgctcgtttt tgtttagatg tgcgtccaaa tcatgaagtg
     1801 acagccatct ttcccgaaaa caaaacggta gaagtcgtac atgagggtca aaaacacatt
     1861 gaacagtacg atgcattggt tttatctcct ggtgcaaaac cagttgttcc atcgattcca
     1921 gggataacag aagccgacaa tgttttttct attagaaatg taccagatat cgataaagtg
     1981 atgcatgcat tagaaaaaca gccaaagcgt gccgtgatcg ttggtgcagg attcatcgga
     2041 ttggaaatgg cagaaaacct aaaaagaaga ggtttagaag tcatggtgat cgaacaagca
     2101 ccacatattc ttccaacact ggatgaagaa atggcagctt ttatagaaaa agaattgtct
     2161 catcaaggag tagaagtgat tacttctcat gctgtcgctg gatttgaaga ccacgggaaa
     2221 cgattgcgac tggatgatgg gcgtaccatc cctgctgatt tagttatttt atccattggt
     2281 gttcgtcctg ataaccagct agcagtgacc gctggaatcg aattaggcat acgcgggggt
     2341 atcctagtag acgaacgtta tcaaacgaat attcctgata tttatgcggt gggggatgct
     2401 atcgttgtaa aacagcaaat cactggaaaa gatgcactta tttctcttgc ttcaccagcc
     2461 aatcgtcaag gtagacaagt tgcggacacg atttccggaa tttctcgaag aaatcaaggc
     2521 ggtattggaa cagcaattat acgaacgttt ggaatgactg ccgcatccac cggtttaagt
     2581 gaaagaacag ccaaagaaaa cgaactgtct tttgaagtca ttcatgtatc aggaaaagat
     2641 catgcaagct attatccaga agcaacagat attttactga agttgatctt ccatccagag
     2701 actggcgaga tttatggtgc acaaggtgtt ggggcaaaag gtgtggataa acggatcgat
     2761 attttagcaa cagcaatcaa agggcatttg acgatcttcg atttaccgga attagaattg
     2821 acgtatgcac cgccatttgg ctcagccaaa gatccagtaa acatgctagg atatgcagca
     2881 atgaacattg cagaagggct tagtgaaacc gtacaatggc atgaattgcc gacagaacta
     2941 gcaaaaggaa aaattttatt agatgtgcga acagcagaag aattggaaaa gggcaaattc
     3001 aaggaagcca aacatatccc tttgaatgaa cttcgggacc gattaaatga attagacagc
     3061 caacaagaat atatcgtcag ctgtcatagc ggattacgta gctatctagc ggaaagaatc
     3121 ttgaagcagt ctggctacca cgtaaaaaac cttgatggtg cattttcttt atatcaaact
     3181 gtccgacctg aagaactgat atatcctaac aaatgatact cgttaaaaag cttgcattcg
     3241 actcttaaaa gaggtaaact aatcggttcc accactttaa aaaaagagga gaatgtgtat
     3301 ggtcaacaca caaaaacagt tagaagaagc atatctgaag aacgtgtatc aaaagctaat
     3361 tgataaaaaa aacgaactgc gggaatcgat ggatacggca aaaatagaag gaaaccagtc
     3421 gttgaaagaa atggctggcg agcttcgatt gaattttgac agttacttag ataacctgga
     3481 tactttttcg atgattgaaa tgaaaaatag agaaatcgat cagatgaata tcaaacagaa
     3541 aaacgcagaa aatcagctag caaagatcga acgtttattg aaaagtccct actttggaaa
     3601 agtcgttgta gactttctgg ataatgagcc aaaagaatcc ttttatatcg gtacagcaaa
     3661 tttcacagat gatgcagatg aaaatcttgt ttatgattgg cgttcaccaa ttgccgaact
     3721 tttttacaat aacatgattg gtccatcttc ttatacggtt cgtcaaaata aaatcgatac
     3781 ggttattaaa gaaagacgac aatttctttt ggaaagagat cgtttggtca agttttttga
     3841 tacgtctgta gctattcaag atgacgtatt attagaagtc ttaggccaag atgagaccaa
     3901 tgaaatgaag gctatcactg ctacgatcca atcggaacaa aacacaatca ttcgggatgt
     3961 gaaaagtcaa aatatcttgg taaatggcgt agcaggaagt ggtaaaacct ctgctatcat
     4021 gcagcgtatt gcgtatctgc tctatttata tcgagatacg atgacatccg atgacttgct
     4081 gattctatca cctaatcatc ggtttatcga ctatatttcg aatgtcctac catcattagg
     4141 tgaaagaaat ccactgaatc taaccatcat acagctggtt tcacagcttt cagcagagga
     4201 aatagaaggt gaagaggctt atttcaaacg tatcacggga gaaaatgtat cggagcagac
     4261 agaacgtttg cgtagcaaaa agtttatcga taatctaaaa caatcagatc ctttgttttt
     4321 agatcatccg aattttatcc gtggacttac taaaaatgga aaaacagtcc tttctaaaaa
     4381 aacgatcgaa aaaatatatg agaaagttcc tgctcatcct aaattgatcg ataggcttca
     4441 agcaacgaaa aaggcactga tgagcgaatg gaagaatcat cttttgaaac aagcaaaaag
     4501 tcctgctgtg cagaatcagg tactctcgtt gacagaagat aggcagttgg aattattcgg
     4561 taaattgatt tctgatgatt cggaacaaag tattgtagct tatgcaagga aacttttaca
     4621 aaaaaaatat cgaaaaataa ccagacaaat cgaagaaatg gcatgggtgg atgagcatca
     4681 gttgtttgaa agaatttatg aaaaacgata tggctcagct tatgcttggc aacccacacg
     4741 tactgttgat gaagcagtca ttatattagc tattcgtcat ttacttgttg aaaaggtaaa
     4801 tgtaccagca tttcgctatc tattgattga tgaagtacaa gattataccc tggcacaatt
     4861 aggtctattg atcgaattat ttccaaaaac tcactttact ttagtaggtg acgagaatca
     4921 ggcaatcttt aattcttcca ctacttttgc tgatattatg cgttgtttcg acgactacca
     4981 tctgccgatc catcggtatg atcttagaaa tagttaccgt tctagcggag caatcacaga
     5041 gcttttcaaa acctatgctg tggatcaaga gaagatcgac atcgtatcga tccgaccgca
     5101 aggaaaagaa cccgaatacc gctcttttcg atcctctgaa gaattgttga aaatactagc
     5161 tgagattttg gtttctctaa aaggagaaaa agcggcagtt atcacccaaa cagaagagga
     5221 aacccaaacg ctacaagaat cgattaaaaa aaattcatat ggattagaaa actgccaaat
     5281 cattcctttg agtctagcaa aaggactgga atttgatcat gtaatcttat atccatttga
     5341 aaacgatgga gatgagcaaa gaagacgcag acaaatgtac acggcgatat ctagaggaat
     5401 gaaaagcatt gtcgtattgg aaagagcaac ataattaaga gataaaaaat aaaaagaaca
     5461 actgataaaa agaacaaaat atttttcatt tgctgatgag aaatgttttg ttctttttct
     5521 taggaaaaat ttaggaacac tttatgtgaa aagaaatgaa atgtatggta aactcctacg
     5581 tcgtaacaaa aaaacgtgag gtgaggatat gaaaaaagta acaagagaag aagttgcatc
     5641 aattttggtg aaagataagt atttttcaaa aggtaattac tggttaaaga tttggcaaac
     5701 gttagtggct cttttaggat ggatgatcgt tgttcttcct tttatatggg tctttttccc
     5761 attaacgaga ccagaaagag ccaaggattt ttccttgtac gcttttttat cagaaaagaa
     5821 aatgctgtat ttcttaatcg gcttttttgt attgattttt ttcagtttcc tgattacctc
     5881 atttgtattg actaggtgga acaatcgaaa tctatacaaa aaagtaaata aatcttttga
     5941 atatgaagat gaaaaattga aaaaaagaca agaattactt gaagaagtgt atacggaacg
     6001 ttttggaaca aaagaagaga gagaaacggt ccgtttttat tctgtctccg aagagaaaaa
     6061 tctagatacg acattcattg cagatttgta taaagaaaat ggggtggaac tgaaatgatc
     6121 tcaatcaccc atacaggtaa tttattctta gatacttgtt tgtctattat gtattttttt
     6181 ctcgtttctt atccgatttt aggcggattt gtatggttta ttggtgtctg gtgttatgtg
     6241 tttatataca aacataagca aaaagaatgg gtagatgttc ccttgagtgt cgaaccgttc
     6301 atcacgatca tggtgccagc ccataatgaa gaaattgtga tcgaatatac aatcgagtat
     6361 ttgatgacga aattgaatta tcacaactat gaggtattag tgacagacga tggttcaacg
     6421 gaccaaacac cagaaatatt agctagactg atgaaaaaat acgctaatct tcgtgtggtt
     6481 cggatcgaaa aaaataaagg aaaagcccac gcgtttaata tcggacttgc ctttgcaaaa
     6541 gggaaattga ttttaagcaa tgatgcagac acggtaccgg agccagatgc actgattagg
     6601 tatgtgaatt atttcattcg ccctggtgca agacatattg ctgcggtcac tgcaaatatg
     6661 gatgtccaaa atcgtaccaa attgattgca aaatctcaaa cggttgaatt ctcaagtatc
     6721 gtgggcatca ttaaacgaac acagtcagct gttttcggtg gtctttatgc gtacagtgga
     6781 gcaaatacga tgtatcgaaa agaagccttg atcgatgtgg gcggattcag acaagatcga
     6841 gcgacagaag aaatcagtat tgcttgggat catcagttaa atgactggtt atctgttttt
     6901 gctcctgaga ttatcttttt catggaagtt cctgtgacgt tgaagatgct atatcgacac
     6961 agaaaacgtt gggcaaaagg tggaacagaa gtctggctga cgaatttcaa aaaagtgatg
     7021 ctccatcctt ttaaacatat tggaagaacg atcatcttca ttgatcaaac attgagtatt
     7081 gtctggtcaa tattcttttg tatttccgta gttctttttg ctggattaat cgggcattat
     7141 gtatatcaag gaaactacga acagatttat attactttta cattcagttt tgtgttcatc
     7201 tgctttgaaa tggtcgcagg atttttccag ttgctagctt cgctgattgt agatgatcga
     7261 cgaagaaaat tgaaatacct tcttttcgca ccactatata tgcttttgtt ctggatagtg
     7321 aacgcgataa cgatcgtgac gactttcatt ccagcagtca aaacgatttt aggttatgga
     7381 agcgggacat ggaaaagccc ggaacgtaca aaaaaataaa agatgcaccg ttttttagac
     7441 aaacagcctg ttgtgtttat ggttcttcca aacatttttg cttataataa gaaattagtt
     7501 aaaaagtagt aaggatggga aaagtgatga aaaaaagaat gaagccgtta atctggccaa
     7561 ttgctgtttc cagtattttc ttattgggac agacagcttt tgcggaaact tctgaacctg
     7621 ctcaaacaga accttcaaca accataagct cttctggtgc tgaaaacggt gcctcggaaa
     7681 cagaaatgac ttccaatgta ccgacagata attcaggaga aacgatttca tcttcagaag
     7741 atacatcaat aaatagttct acctcttctg accaaatcga tcatttgcaa gacttggaaa
     7801 ataaaagaaa agaactcgaa gaagcattag aagctgcaaa aaacaaattg aaagaagacc
     7861 aagcagcaat cgatcaagat ttagctgatc ttaaaaataa agtgggaaat gaccgtgcac
     7921 aacttgagaa aaagcttgcc gaagcgaaag caaaagcgga agctgtccaa acagatcttg
     7981 acgggaagat ccaacaagca aaagaattca atcagcaatt aaatcaggtt gctcaaacag
     8041 cttctggaaa gctaagtcaa actcaagcgg atatcacagc ggccagtcaa gcaatccacg
     8101 gaaagatcag cgaagcacaa acagttgcta atgattttga agcaaaaaaa gctgacttag
     8161 cagctagttt ccaacgaata aaagatattt tgcattcggc aggagaaaaa tcagtgacct
     8221 ctggaagcac gacagaaaca gttcagcaaa cgacagagcc atccaaatca acaagtcaga
     8281 ctttgccaaa aacaaatgaa aaaacagctt ctgtcagtta ttccttagca ggggcgggtg
     8341 tgttagtcgc agcagcttac ggaatcatcc gcaaaaagaa ataaaaaaga gaggatggga
     8401 aggtagagta cctcgaagtt agactgaaaa atctaattct tgagtgccac tacatgtccc
     8461 atctctcttt agttattaaa acagatgtcc cctacttatc tcgtggagac agctgttcca
     8521 ctacatgtga ccatcagcat gccattgtcg ctgcctaata cttcatagtc catttttacg
     8581 ccaatgatcg catcagctcc taatgctttt gcacgttcgg ccatttcggc aagtgcttct
     8641 tctcttgcac ccattagttc gttttcatag ctttttgagc ggccgccaaa gatatttcgc
     8701 aaccccgcct ccaagtcttt aaatgcatgg atcccagtaa tgacctcacc aaaaacgatc
     8761 cctttataag ctactacggc ttttccctct acactgttcg ttgttgttgt aatcattcga
     8821 tcactcctct cttttaatta aagtataaca aacttcaaga agcactacac atattttccc
     8881 aaaattagaa atttattata tacttgaatt gttcattaat gaaagcgaaa ccaatcgtta
     8941 gcaactatgt aaaagaaagg aagattcttg tgaaaaaaag tattttgctt atttctcata
     9001 gtcaaaaagt aacagatggt attaaagaaa tgattgagca gatgcaaaaa tccgaagagg
     9061 tgcaaatttt ttctctcggc ggaataaatg gcgagatcgg atcagacccg atgaaaatcg
     9121 tagaagcagt caatgaatca agcgatgcag ctagttattt tgtttttgcg gatatcggta
     9181 gcgcggtatt gaactcggaa ctggcaaaag acatgttgga agaagagcag cagcaacgtt
     9241 atcatctggt ggatgcgcct ttagtggaag gagcttttgc agcagccatc actgccggta
     9301 taacagatga tactcagcaa atcttaacag aagcacaaaa tgccggaaag aaaggatggg
     9361 aataaattga aaaaaatcat gaatgatcca agcaatattg tcgaagaaat gctggaagga
     9421 ttagtaaaaa gctatccaga acttgttcat cgtgtagaat cttcccgtgt tgtagccaag
     9481 aatcagaaag cggaacaagt tggtttggtt tctggtggag gaagcggaca tgaaccatcc
     9541 catgccgggt ttgtagggga agggatgtta agtgcagcgg tgcttggtga tgtgtttact
     9601 tctccaactc cagatcagat tcagacggct attaaggaag ccgatagtgg aaaaggagtt
     9661 ttgctgatcg ttaagaatta tacaggggat gcattgaact tcgatatggc aaaggaatta
     9721 gctgctatgg acgatattga agtagaaagt gtgatcgtag atgatgatat cgccgttgaa
     9781 aatagtacgt atactgctgg aaaacgcgga gttgcaggta ctgtccttgt acacaaaatc
     9841 gtgggcgatg cagcaagaaa cggtgcttcc ctagctgagt tgaaagaact gggagaaaag
     9901 gtcgtacagg caactaagac gatcggtcta gcgcttcgag cagcaactgt accagaagta
     9961 gggaaaccag gattcgaact aggagaagat gagatcgagt atggtgttgg tatccacggt
    10021 gagccaggct atcgtcgaga aaagatgcag ccttctaaag tactggcaaa ggaacttgtt
    10081 actaagattt tagatgatta ctcgaattta ccaaaagaag caggtgtact agtcaacgga
    10141 atgggcggga cgcctttgat ggaacagttc gtgttcatga atgatgtttt agctctgttg
    10201 gaagaaagag gcgtcaatgt agttttccga aaagtcggta attttatgac ctcactagat
    10261 atgcaaggtc tttctctaac actgatcgac ttgacggaga cacaatggaa agattcttta
    10321 gaaagcaatg tacagacgat ttcatggtaa aaaatctcaa gataaaaggg tgataatatg
    10381 aaactaaccg ttcaagaaat ccaagaatgg ctcacgcaat ttgcagaggc aatcaatcag
    10441 aacaaacaat atctcagtga tctagatacc ccgattggcg atggagacca tggaaataac
    10501 atgggaagag gggtttcagc ttatgaagaa gcttttcaag ccgatcatcc tgaaacaatc
    10561 agcgatacat tcaaagtatt ctctatggcg atgatatcga aagtcggtgg ggcttctggt
    10621 ccgttatacg gatctgcatt catgaacatg atgaaagcga caaaagaggt cgattctatt
    10681 gattctcaag aaaaattagg cgaagtgatc gagcagggaa cagcgggtat tcaaagccga
    10741 ggaaaggcag aagctgacga taaaacgatg ttagatgttt ggctgccagt cacagaggca
    10801 ttgaaggcag ggaatttaac aaaagaaatc attgaccagg caaaagaaca caccaaagtc
    10861 ttggtagcta aaaaaggacg agcttcctat ctaggagagc gtgcaatcgg acatatcgac
    10921 ccaggagctg cttctagtgc cattttattt tcaacgcttt tagatgtgat caatcaacct
    10981 taagttcaat aaaactgcgg tgatgctcat cgcagttttt tgtttggctg ttcttatgac
    11041 ttgatgcaga acagcttttt cacaacctct atttgtccat atcgcaaaat tcaggtagaa
    11101 tacaaaagat aacggaaaga ggcgaaacaa tggaacttgg aaaatcagat tatcagttgt
    11161 ttgatcgacc aatctacgca ttcaaacagc tcaaagagag ccatccaaca gataagattg
    11221 aacaaatcaa gaaagaatat aaagaacact ggcaaaaatg gaaagagatt cagctccaga
    11281 cagctgcact tttgcctgat atgtacggca tgagcaaacc taaaatcgaa agctggacga
    11341 atggctggaa tttacgcagt catttttgga gcgcgtaccg aagcgaatct cgtcaagacg
    11401 agaatgcctg tctagcagtt ttgttgaatc aaaaacaata ccaaatctat ttgatgtacc
    11461 aacattataa aagcgaagaa cgatatggta gcattgcagc gtataaccag ttgcttgcga
    11521 tactgaaaga gtggagcaaa acagtcgata tcaaggatta ttacatatgg ccgcaaccag
    11581 aacatgaact tgacgaccac ttagctttgt ctgactattt gtccgataca aagaaacaag
    11641 aagagttgaa aaaagcgatg agagaccgaa cctttcaaat gggtaaacta ttcttctatc
    11701 cgcaagaaat cgaacacgtt gagcaaataa ccgctgaaac cttacaagaa ctagcaccac
    11761 tatatcaaaa gctaggtgcg gaaaaatttc aatagacaac acgaaataag caatgcgaaa
    11821 aacaaacctt actctcggct tgcgagtgaa ggtttgtttt tgattattca aaaagtaaat
    11881 atttaaaaaa ttgataggaa tcaattactt aaggtctcaa aagaaggaaa ataaagcaag
    11941 ggacaaaaag aaatccactc tatttgctgg tatctggttc gcttgacgtt tatgctatga
    12001 taaaagaaaa ttatcagttg gaataaggga agttatgttt ctttattaga aatagctgtt
    12061 tagaaggagg agagattatg agtcatgatc atttatgtgt ttcatttgtt cctttgttca
    12121 atcacttagt acagcaagat caagaaaaag tacacgcact tgtcaaacat cactatgtgg
    12181 aaaaaggaga acagattctt gcgccgaatg gagaacctca attagtgatc gtggcctccg
    12241 gcagtatgaa agtgtatcaa ttatctgcta gcggaaagga acagctttta cgagtaatag
    12301 agccgggcgg atatgaggga gagagccagt tgctaggcgt tccaaatgag tctttatttg
    12361 gcgaagcact agaaaaaacg gaaatttgca tattgagaca aaaagatttc tcggaactct
    12421 tactgcatta tcccgccctt agtctgaaac ttttagcaat caacgttgag aaattaacaa
    12481 aagtacagca gcagacaact tttttgatga tggaaaaagt agaagaacga gtcgctgtct
    12541 atctgcttga cttagctaaa gcgacaggga aggatcactt tttcctgcca atgaaaatga
    12601 aagaacttgc tgcatttatc ggtacgaccc ccgaaacttt atctagaaaa tttaaattat
    12661 tagaggagaa tggttatgta aaaagaaaga atcgagaagt ctggatcatt gataaagagc
    12721 agttagaaga cttgtaaata aaaaaatgac ctccaaatgt tggatttttg acccagattt
    12781 ggaggctagc atgaaattgt acatgctttt ttattatcct tctagtaagt ctcttcgcct
    12841 ataaccaata gaaccaagga cgaacagtgc aaggctgata catgataacg taaggaacac
    12901 ggaaagatca aaagaatcgt ttggcatccg tggaacccag ctagggacag ctgttttgga
    12961 aaaccattct ggtaaatcca aaattccgcc aaagtaattg agtgcaaagg agtaagctaa
    13021 gtagatataa gcaagtttac cgattcgagg aagccatccg aatagaaagc cactaattcc
    13081 gataaacact aaaacggcag gcaggaaatt gaacccggct ttaagaaaca tcaatagatc
    13141 catgtctttc gcatccttca tcgaagccaa agcagaaagc cctagtgcac cagaactaat
    13201 caggattgcc aatatagcaa atagaatagc tagtaacata ttggtccaat aaagttggtt
    13261 cctagaaatt tttgttgcga acagctgatt catacgtccg tgggtttctt ctgtaaataa
    13321 acgattgatg ctagcaattg gtaaaatagc agctagacaa atcaagacga ccatgatcgt
    13381 gcttgtaaag tttttttcaa ttgagacgcc agattggata aacatctgtt tcattaagtc
    13441 gttactttct aaaaactgtt gcatattccc gtagatagag ccgtatgccg cccccatcaa
    13501 accaaaagtg ataagccaac tgattgtcat ccctttattt agatagaata ataaaccacg
    13561 caaggataaa agagaacgtt tagcatacgg ttttccttca cgttcgggga tatagccggt
    13621 gttcaaatct cgatgttcct ctaaaacaaa tgaactgatt atcaagatta gactgaacaa
    13681 aaacaaataa agcaaaggaa ttcagttgtt agttgtaaat ggataggtca aataagacca
    13741 gcctaaaggt ttccacatcg aaagggagga atcagctaca tctgttattg ctcgtagaag
    13801 gtaaagtcca cctaaaaacg ctaaagacag accattcgaa ctggctgaac ttggcatcag
    13861 ttgtgcagca aacagtgcaa cggcactccc caaaatcccc atactaccta cagatagccc
    13921 aaaaagccaa gcaccttctg cagtgatcga atccgcttgg aaaaaaatca tcgtacaact
    13981 gatcgatatt gctaaaagca aattgatcag aatactttca gcgatgacag ctaaagagtt
    14041 tgcttgacgt ccgactcgaa aggaacgaat aagttcaatc agaccaagtt cttctttttt
    14101 tctcgtttgg ctcaccacgt gtaaaacaga gatcacagca gcaaacaaac tgcaaaaaag
    14161 caacatttca tttgcataca tcgctcctaa agtatactca gcagcattcg aagctggagt
    14221 tggtccgacc attgcaatca ttgccgggtt ttgcaaggtt tcgtataatc cagctaatcc
    14281 ttgtccggaa gtcaattctt caaacgcagg gacaaaacca ccagaaaaag cagtgacacc
    14341 gactagccat atcaggatct ttttccagta acggctaagg ttttgagaaa aaagggtgga
    14401 ccagcgtgaa aatttatcag tcatttcgct tcctcctcat ccttcataat gtctcatgaa
    14461 taaatcctca agagtcggtg gaatagattc aaattttttc acacctaatt tggacgcttc
    14521 agctaaaatc acatcgaaag cttcgttatc agcagcaaat tgagcgaggt gacctttttg
    14581 cacaaagtca tggacaccgt tcaattgtgc taagtgacta ttctctgctt ctgtttctat
    14641 agtgatggtc gaacgagtca aatgacgcaa ttcatcgagt gttccttctt catcgagtgt
    14701 tccttcgcgt atgatcacga ctttatccgc tagtcgctcg acttcactga gaatatgaga
    14761 agaaagtagg atggatttgc cctgttcttt caatttttca acttcttctt ggaaaatcgt
    14821 ttccatcaaa ggatctaaac cagaagtagg ctcatcaaat aaatagagat ccgaatcaac
    14881 agccaaagca gcaatcaagc ccactttttg gcgatttcct tttgaatagt tttttgcttt
    14941 tttcttagga tcaagagaga aacgttgaat cagttcatct tttttttgag cactcccttt
    15001 tccatgaagc ttcatgaaga gatcgatgat cgctcctcca gtcaaatttc cccacaaaga
    15061 gatatcgcct ggaacataag aaagacgttc atggatagat aaactatctt cccatacatc
    15121 ttgcccaaaa atcgttgccg ttccgccgtc tttttttata atccctaaaa gtgtccgaat
    15181 cgtcgtggat tttccagaac catttggtcc gataaatcca acgacttcac ctgaatgtac
    15241 ggtgaaagag acatctttta aagcttcgaa acgaccaaag ctctttttca aatgtgtgac
    15301 tttgacgatt tctgccataa aaaccccact cctttttcta aaaatatata ctaaacaaga
    15361 ttaaatagtt taataaaaag gttttatacc tttttataaa acttttttta ttattttagt
    15421 acataatatt ctttttataa atttctgtca atgaattcat aaacaaggaa ttgctagctt
    15481 gatactttct caatagatag ttataataaa cctcagaata aatttgaact atatatatta
    15541 taaaagttta aaattacggg aggctctaca aatgaacggt tttgaaaaga gagcacaaga
    15601 gaaaaagaaa gagattttac aagcagcttt tgacttgatg aacacagaca taggaaccgc
    15661 tggagtgaca atggataagg tagctgagaa cgctcaggta agtaaagcga cgatttttaa
    15721 atattttggc agcaaggaag ggctgattta tgaagtattt tttgcattta tgaatgattt
    15781 agggcaagaa gcgaaagcgt tgattgcgga aaatcttcca tttgaagaaa cactgattgc
    15841 catgagtgaa aacaaaatcc gttatctcaa tcgagtgaac aaacaatttt atatcgattt
    15901 gatgacatac tatacaaaaa aagaaaaaaa agaattttct gacgcgatgg atgcgtatac
    15961 gcaagaaagt ttcagtatca tgcttgatct tttccatcga gggcggaaag aaggaaaagt
    16021 ggatttgaaa tattcggatg agttcttgat cctctatttt caagcaatgg tagaaggaat
    16081 ttctaagccg gagatatacg caaaactgct tccatataca gcagaatgga cagaagtcct
    16141 gatcaaggga atcgcaccga agaagaaata aagcataaaa aagccgaaac tgacgataaa
    16201 atgtcagttc ggcttttttg gtgtgcgccc tgcatgggtg aaaacttggt ggtgaaagtc
    16261 cactacaggc ttggcagtag gaactgttag cgaaagacaa ggcggcaatc gtgaggttgt
    16321 ggctgaagga agtcggacgc aaactcctga actgacgaac agaaactaga tacaaggctg
    16381 aattaggtcg gataagtctt catcacaaga caaagtccaa tactgcccga aacctataca
    16441 gtaaatctag cagttacatg ggaggaaagt tttcactctt accgggggag atctcatgag
    16501 ggtgttgtgg gcgttgaatg aaaacaaacc agcacaacga tcatggcgac atgatggtga
    16561 atcatgagaa gtcagccgag gtcatagtag taacgacact gaccgttacg aaggactgaa
    16621 caataagaat cttgaataat ttaggaggtg tgagaagatg cgacgaccgc agaaaacaag
    16681 aaaacttggc tatcatcaaa gagctagtgt ggaacacgaa gggtatgatg aagtgcgcag
    16741 cacttcttgc ggtgaacaga ggaaacaaga tggtgtagaa aatctgtttg ggagaattct
    16801 agatcggaat aatctcaatc aagcgtatct tcaagtagtc agaaataaag gggcggctgg
    16861 tgcagatggg atgaccgtcg atcaactgct tccttacttg aaagagcacc gcgaggaatt
    16921 actatccaat ttacgccaag ggaactataa acctcagcct gttttgcgag tagaaatacc
    16981 gaaaccaaac ggcggtgtaa gaaaactagg aattcccacg gttattgatc gtatgcttca
    17041 acaggcaatc aatcaagtgt tacaaccgct atttgaacca gaattctcag ataatagtta
    17101 tgggtttcgc ccaaatcgca gcgcacatga cgcagtgaag aaagcaaaat cttattatga
    17161 acaaggttac cgatatgtgg tggacattga tatgaaagcc tactttgaca ccgtaaatca
    17221 cgataagctg atgtatttca ttgagcggaa agtaaaggac aaacaagtcc taaaactgat
    17281 ccgtcaatac ttaatgagtg gtgtaatgga aaatggactc atcacaacaa ctgaagaagg
    17341 aacgccccaa ggaggcaatc tctctcccct gttaagtaac atctacctga acgaatttga
    17401 tcaagtgcta gaaaatctag gacataaatt tgttcgatac gccgatgact gcaatatcta
    17461 tgttagaagc aaacgagcag gacagcgtgt gatgaagaaa tccattcaat ttctcgaaga
    17521 acaactaaag ttgacggtaa atcgagaaaa gagtgcggta ggaagtccat tgaaacgaaa
    17581 gtttcttgga ttctgcattc tgccaacaaa gaaaggtatt aaaattcgcc ctcacgcaaa
    17641 agcaaaacag cgtgtgaaaa gcaagctcaa acaactcact aaaagaaacc gtggaagaag
    17701 tatccaactt attttaaaag aaatcaagca actaatgact ggttggatca actactatgg
    17761 aattggtgag atgaaagaat tcatgaggga gttaaacggt tggttgaagc gaagaatcag
    17821 acaatatatt tggaaacaat ggaagaatcc aagaaccaaa agaaaaaatc tgattcgttt
    17881 agggatcgat aaagcgaaag cgtatgaatg gtcaaataca agaaaaggta tttggtcagt
    17941 ctccaaaagt catattcttc atcgatcatt aacagacaaa gaactggccc gccaaggata
    18001 tgaggacatc tccttgaagt accagttcgt acactcaact tattgaaccg ccgtatacgg
    18061 gtccgtacgt acggtggtgt gagaggggcg gaaggtcagt t
//
LOCUS       DAFBQH010000053        17920 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.055, whole genome shotgun sequence.
ACCESSION   DAFBQH010000053 DAFBQH010000000
VERSION     DAFBQH010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.055"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(96..524)
                     /locus_tag="LK694_001900"
     CDS             complement(96..524)
                     /locus_tag="LK694_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052747.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(552..1190)
                     /locus_tag="LK694_001901"
     CDS             complement(552..1190)
                     /locus_tag="LK694_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5052748.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1194..3176)
                     /locus_tag="LK694_001902"
     CDS             complement(1194..3176)
                     /locus_tag="LK694_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBK5052749.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3356..4021
                     /locus_tag="LK694_001903"
     CDS             3356..4021
                     /locus_tag="LK694_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5052750.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(5956..9105)
                     /locus_tag="LK694_001904"
     CDS             complement(5956..9105)
                     /locus_tag="LK694_001904"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HBK5052751.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(9118..10317)
                     /locus_tag="LK694_001905"
     CDS             complement(9118..10317)
                     /locus_tag="LK694_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBK5052752.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(10307..11902)
                     /locus_tag="LK694_001906"
     CDS             complement(10307..11902)
                     /locus_tag="LK694_001906"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBK5052753.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(12486..13193)
                     /locus_tag="LK694_001907"
     CDS             complement(12486..13193)
                     /locus_tag="LK694_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052754.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(13258..14094)
                     /locus_tag="LK694_001908"
     CDS             complement(13258..14094)
                     /locus_tag="LK694_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK5052755.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(14112..15521)
                     /locus_tag="LK694_001909"
     CDS             complement(14112..15521)
                     /locus_tag="LK694_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK5052756.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(15535..17358)
                     /locus_tag="LK694_001910"
     CDS             complement(15535..17358)
                     /locus_tag="LK694_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK5052757.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         5489 a         3620 c         2849 g         5962 t
ORIGIN      
        1 atacaactaa aaatgaatat attaaaacta ccaaaaataa cttttaacgc tgtttcatta
       61 tatgtttttt ctattttata taaacttttg gatacttaat accaaccggt tggattgtac
      121 caacatgaga atgtgtcagt tctatttcct gtcgcacttg cataggccca tactcctttt
      181 gttgcatttg ctgatcgaat agagtttcca actttcgctt ttgcaccgtg acgattatag
      241 acacgactat agtggttaga atgacctgtt tttctcgtac aagttgtatc actataataa
      301 acccatcttc cagagctatg attaattgct gttctagaaa tttcccggtc tactggtaaa
      361 tttgacttaa taatattacc tttagcatca aaatgaatct ctcctggctt taaagatttg
      421 taatagattt caccgtgaac tgaatttgct tccacttcag atgacatagc gtttcctcca
      481 acaaaaccta tccctagtgc acaaataata actgcttttt tcataaaacg acatcctttc
      541 ctttttgatt tttaaagcat gatcttcttg tttcctattt tggtcagata tggatcatgc
      601 gtgactacga taactaacgt accttgggaa gcttgttgcg ataagatttc aactacttta
      661 tccttatttc ttttatccaa tgacccagtc ggttcatcgg ctaaaatgat tttgctattc
      721 ttcaataaga ctctcgcaca ggcgattctt tgcttctcac caccagataa tgtgtagata
      781 ggcgtactta gcttcttgtt tatatttact tttttcaatg cctcatactt tgctgttcgc
      841 ttatctgaaa catttaaatt cgaaaacttc aatccaatat ctaaattaaa atcgattgtt
      901 tcatattcaa tcaacccaaa atcttgaaaa agaaaagata tggtatttct ttttatcaag
      961 cgcttctctc tcccagttaa ttgtttcgtc tcttgatcga acaaatagaa ttgtcctgaa
     1021 ctgaactgat cattcaatcc taatatattc aataacgtac ttttacctgc tccactttct
     1081 ccaacaacag tcacaagatc cccagcatcc gcagtgaaac gatccaaatg aagaatcgtt
     1141 ttgtttcgaa tgattttttt cgcacctttt atcactatca acggctccaa ttgttaacct
     1201 ccattcatca gcaataactg acttttcttt tctgtagaca aaataaaaac aactgaaaga
     1261 ataaaatcaa tgaagctaaa gaaaaaaata ttaaaaagat atttaggctc tctttctata
     1321 agaaaaagga agattacact tgtcgttccc catttgatga ggttcatcat gaagaaatct
     1381 ttatacgttt caaagagcga ataaccactt gaacgaaaga tagcgatttt ccgacaattg
     1441 tagacaaaat atatgcataa catgacatag ctcaacaaga agaaagaaaa gaaagtgagt
     1501 aaaaatatct gtatcaagta atgaaatgat ctgcttaaca ctttgacagc tatctcttca
     1561 gtctgagtat acggaaatga taactgcaag ttatcacgta actgactttc ttcaatcagc
     1621 tcaaataacc tttctttcga caaagagcct atcttgatct ttaacggtgg atagatttct
     1681 ccgctgagaa tatttctttc caaatacata ctgtttttca atgtcagtat ttcaacaacc
     1741 ggataatgtt cgatccaggg gatagaagga ataaatgtat aaatgggttg attgtttttt
     1801 ataaatagaa atttcggttc tttttcactg atttcttgat aatattctgt tatttgcgcc
     1861 aggtcatttt ttaaatctgt atgtctatat cgttcaggga tcaatataac agggtgcgtt
     1921 tctttttcgg ttatttggat caaccttcca gagacatcaa tcaaaggaaa tttcttaaga
     1981 taattcagat ttatttttat ccctctgcca aactgttcat tttcttcatt caagtaatct
     2041 gatacattga ctaatatact tccgttttta ttcaaagttt catataattc ctgatctgct
     2101 gctgatgcaa aacgattgga ataaataaaa tccacgtggt ttttcccgat ggttactgga
     2161 taaaatacat gatagtcctc accagcgatt tcttttggtg gttcatggga aacagctaac
     2221 tgtgaagaag aaaaaacgat agttgctaat tcgacattgg caacggtcaa aagaagagaa
     2281 aacaacaatc caaatgtaaa aagaatgtat tggatatagt tgaacgcttc tcttaacgaa
     2341 gcgatttttt caactaataa aagagaaatc aaagagatac catatattcc gctgaacaag
     2401 aaaacgtaca tctggaacaa ataaagaaat tgctccttat aatacataaa aataaaacca
     2461 ccaatgattc ctgcaattat gaatgtaata atgattttct ttttccccaa taaacaccac
     2521 aatacttgat gtaatgaaaa gtcattattc aacaataaac tgatttcttt attcatcaat
     2581 aagaaaaagg atgataacaa aaggaataaa agaatcaatc caatagttaa atacactcga
     2641 taattatata cgtctgtatt tgaaataacc gaatacgaat agctattttc aaagtcttca
     2701 aaaatattag tggcataatc agtgtgaaat gcttgattga atcgatcagt gaatagtgaa
     2761 atgacccgtt cataggcttc tttttctctc gtcatcaaga aaaattcacc ttcaaaatct
     2821 tttattttga tcgattcttc aatggacgtt acgcgaaagg aggtattaga atagggtacc
     2881 ttctcgtgag aagatttgac atccccttga taatacaatg tcatttcaag ctttggctgt
     2941 atcttgaagt tccaagatgt atctacttca ttggattcat ttactgtttt tttcatataa
     3001 gaaagctcag tttctgatga gacattttca aataccatga ttagctgctc atattcttct
     3061 tgcccaataa aatgttgtgg caatcgaatc ggagtataag tatctgtttg atggaaaaat
     3121 tcatttgtcc gatagagtac ctgtttattt atactaacca aggcaaaacc actcatgata
     3181 ccggtcacta tgattgtgag taaaactgtg aacttttttt tgattatcac cactctccct
     3241 tacttaaatg attctttgat aaaatcatac cgtttttcat caaaaaaacg tatctatcaa
     3301 gtgtataggt catgtagaat aaatctatat actgtagaat cgaaaggaga agaagatgaa
     3361 caccggagaa aaaatacgaa cttaccgcag aaattgtaag cttacgcaaa aagaattagg
     3421 cgaaaaaatt ggtgtttccg ataaaacgat ttctagttgg gaaaattctc gtacaatgcc
     3481 agactttgaa atgctgtcac ttttacatca tactttaggt ctgccgattg attttccttc
     3541 tattgaatca gctaccgaat caatagaacc aacattgcca gatgaacctc ttgttaacaa
     3601 gaaatcttat tcatcaaaac aacccttctc actcaaaaag acactatctt tctttctgct
     3661 cgtcttttgt ttggtctatc tttttcttat caattatcaa tacaaaaatg aaacgatcga
     3721 tcgattcaat ccttttttga aaccacaaat cggctatacc acactccctg taggatacgt
     3781 ggacaattcc atcgaacgtt gggtatgtaa tgatatcttt ggccatggta catggctgtc
     3841 ttttcacgct ggagaggtag aaaaagagaa aaaccatgct gtaatcaaac acaaaggaaa
     3901 acacgtcatg aacgttcgcc tgctcacttg ggaagccatt ccctcagatt accaagaaat
     3961 cctcagccaa gactatgacg acgccttaaa aagaagccct caggagccag ataggcatta
     4021 agtaggagat gaacgtagag atagaaaaaa tcaatgcaca tgatcttatc caaaaaacag
     4081 gggtacaaat taattgaaaa agccttgaga tgaaagttcc taaataaaaa agttcagaat
     4141 gataggtatt tctggcttat agtataatat ttacgccaca gcctcccaat gattcttttt
     4201 ggaatcataa tcaaggatgg acattcacaa aaatattttg ctcattcgat ccgaatagat
     4261 taacgaccaa tctagtaagt aatatagatt ggtacgtagt caagctgcac ataatttaga
     4321 tcaattttag ttctttttta tatttctatg atctgctcat ttacttttat atgatggctt
     4381 acagtaacat aaataaatat gataatcgct ggtagtttat ccaagagtac tcaataaaat
     4441 acaaaatgga ttgttcaagc gcctatcata atgataaaat gaaacaaata aacgatacaa
     4501 ggaggaagag actttgagta atggcagaac aattttttca ttgtaaaaat tgttcagttg
     4561 ttaatctatg ggatttcggt agcatgggca tttttttata gaggaatcac accaacctgg
     4621 ccgttttatc tcttgttagt gggaatatta gtaggaaatt ttgcccctct tcactatcgt
     4681 acgaactttt ttccaactga ttctggcctt tttcttaaaa caaagacgcg aaaattagaa
     4741 aacattattg aaatgacaat tacctgtata atcattttct tagttatatt cctaaacgtc
     4801 ttctaataag aatctcttaa aattagcttg ttacagttag ttacctagtt tatataacac
     4861 agttatctta tcttccttat caacatcttt tgccaactct ttgtcttaaa ttcgaaacat
     4921 ataagcagag ctacgaacac caagactgtt tcggctctgt tttttgcatc aaattcactc
     4981 ttatatagct gataatctcg gctctttaaa agaaattatt ttcgcttcat tttattataa
     5041 aaaggctagg aaccttgatg ttatcaggtt cctagccttt atttaagtat taccccacag
     5101 acccttccat atagattttt aaattacaca ctagtttaaa ctaaaacaaa caaggaaaaa
     5161 cttaccattt aatcaaaaaa ttagctcata tgaatatatt agaatgtaaa ttagactggt
     5221 caaaatctgg tcaaaaaaga ggacgaatac aggtagtcct cttttttgtt attttgatat
     5281 tctaaatcat tttttattgg attattccct tgttcgatat agacccaaat tgatctatca
     5341 ataattatca aactgagtca tatagagaca aaaatttcaa aaatgacctg ttaaatggtc
     5401 atcgaatatc atttaaaatc aatacgacgg agtcagacac ggagtcaaaa caagcccgct
     5461 gagagtaaaa aatatacagt cccataggta aagtgccaca ggactgtata tttttttatt
     5521 tgagattacc tgaaatgatt ccatttaatt tttatctcat tttttcctac tagttagtat
     5581 caaatactct cctattttct caataaacta ttttgggtta catggtatct gaaacagcta
     5641 aaaccttgtc attatagcaa aaaatcactt tatctccgtt tcatttcaga tacatttcac
     5701 ttaagcggat tccaaaaaaa caaaagaaaa aggaacccct agttagccta tgctttttat
     5761 tggttcctct caaaacacgg tcatataaca acggggtggg gtaaatgaat gaaaaaggtg
     5821 gaggaaacat tattcaatag ctgtcttaaa tacttcgtta taaaatgtga acaaataaaa
     5881 gacatctatc cctattttct acacttccac ttgactacca tctttgaatc gtacaatttt
     5941 cttaactctt ctcgtttacc tcaattcatt ctcaagtgga atcaactcat catctaacat
     6001 tttacgccat gctcgcttca tttcttgtgg gtatttaaat ggcttcgctt ctggatgttt
     6061 ggctttatat ccttcgtaat ccttactgtt gataattgct ttcatattag gaattggatc
     6121 ttcacctggt agatattgca gattagaagc atgtaattct ttctcagaaa caaaccactc
     6181 attggaaaag acatgaatca aatcatcact cgcttgtgta aaatagcgac gtttaatgac
     6241 aaagatatct tcattactat ctaaagtccc cacatcaaat gcatttaaaa tttgtcggta
     6301 aacttctttg actccttctg ttttattcaa tttagccaaa gctttttcaa tttcttctcg
     6361 tgcatctgaa ctattcgcag cataagttcc tagtaattga tcaatataag ttgaatcaat
     6421 atcatacaac ttagtggtat tttcactata aaattcgatc gttgaaaaat cttctccttc
     6481 tttttctggt tcttcctcta ttaatgaacc cttcacggtc tcgtacacgc caatttgttc
     6541 ttccaaaagt tgaatttgat tgttcaaagt tggagatttc accatgtcat cattgtaatc
     6601 gtcataagtc actaacgatt cataagcacg attcaattct tgaaatgctt tgacgtactc
     6661 gatacggatt tcaagtggcg tatgttcatc aatggattcc atggatgaaa cctcttttaa
     6721 cttaccatat gcttgtttga atcttttttt ggattcctga taacttggat aaatcaaacc
     6781 tgattcttct tttgcttttg aatacagttt tgtcgcatcc tcgacattct tctccattgt
     6841 cgctggctta cgaaacgtca caatcaatcc ttttgtctta tcaggatacg tacgattcgt
     6901 acgtgaaaaa gcttggatca atcctgcata ctctagatta cgatccacaa atagggtttg
     6961 aatcgtaggg gcgtcaaaac ctgttaatag acgatccact acaatcgcta aatcgatttg
     7021 tttcccaaat tgtttgaatt ccgctctttt tcgtgccaaa cgattattga tgtctccgtt
     7081 ataacgctca atgtctgcta atgaccagga agtatcataa tactgattat attcctcaat
     7141 aattttttgc atttcttctt gttgggcact tgaatctcgg ctattttctt ctaatgaata
     7201 ggtaatagcg attcgtggaa aatcaggatc ctccatcgtt cgtccttcac gaatgagctg
     7261 attgggaaac tcttttgtta accaatcagg ctctttcgtc atctctttga tggcccgata
     7321 atactttttg gccatgtcaa tggaactcgt ggttaagatt gcagatttcg ttggcctgcc
     7381 atttcgaaaa tcaaattttg tataggcatt atctggacgg aacattttat gaatgacttt
     7441 ttggatatgt tcatctgctt caaaaacagc tgggtccaaa taactttctt ttttgacagg
     7501 ttccatttga tcaatcatac gattgatctg ttctgatgaa tactctgcat atgtttctac
     7561 ttcttttaat ttccgataga ttttattctc tagtgaagtc ggctccacgg tgttttcatg
     7621 ctctacttga aatcctaaga cagagccatc ttctagcgca ttcttaatgg tataggtatg
     7681 caagacctct ccatactgat catgggttgt acgtgccaat tgccctttgg cttgtttccg
     7741 attctcagga aaaatcggtg tacctgtaaa gccaaaccaa gtggatttcg gaaacatttt
     7801 tttgatttct tccatatttt cagcacttaa cgctcgatga cactcatcta ctacaaacac
     7861 gatatgttgg cccattaatt tttggaagcg attggttcct tttttctctt cttgtttctt
     7921 tgcatatttc aacgccgcat ctagtttttg acgggttgta atgattacaa catttgcatt
     7981 ggcatctgcc aacagggttt cacttaattc tttggcactt cccgttccaa cgatcaagct
     8041 attcgctttg gcgttcccgg aagaaatccc agtattaaat tccgaagcaa attttgtaaa
     8101 ctctgtggtg gtttgattat ctaaatcttt gcggtccact aacatgatcg ttcgatcgat
     8161 ccccgatttt cgtgctaaaa gcttagtaga tacaaaacta gtcaacgtct ttcctgaacc
     8221 agtcgcatgc cacacatacc ctgattgatg tttatttgcg gcaataaaca aggcttgaat
     8281 cgcatgtact tgataaggat gtaacaccat gagggtttta ttatcttgat cttcactgac
     8341 gattgtataa tccgcaatca aacgatgagc atccgggata tttaacactt gtttacaaaa
     8401 ctcatataag ttttctactt tttcattgtc tttcgttcgc caactaaata aaaacttagg
     8461 gtgcaagtct ttcggtaagg catttgcaaa ataccgagtg gtttgttcat tggaaaccac
     8521 aaacaactgt aaggtcgaaa agatattgtt ccgaaacata ccttcttctg catatttttt
     8581 gatttggtta aatgcctgat aaacaccatc ttttgcagtt acttgtttca gctcgatttg
     8641 gacaatcggt aatccattga ttaataaggt gacgtcaaaa cgtcgatccc gtgcttctat
     8701 gttacttcca cgttttgcaa tttgatggac cacttcataa gtagagattc ctccaccaat
     8761 gtcttgattg gaataaagga taagtgagac agaacccaat tgcgaatctt cccgttcaat
     8821 cgtgatccga gccatcccat tttcaccttt taaccatttc gcagcatcaa atggggtttt
     8881 cgttcgcagc agtaattccg ttttgatcgt ttcaaattct ttatcggtca aaggaaaatc
     8941 attcagttct gcttgattat ttgagataat tttttggcgt aaattgaccc aaaggtcttc
     9001 ttctgatttt agatccggac gatacgtcca ctgattatgc ccttctccaa gtacacgaat
     9061 caactgttct tcgacttctg cttcctcacg gtgactaatt ttcattcttc ttccctccta
     9121 gacaaacatc ttttgtaaga agcttttttt cgtttctttt agtaaatcta acttacgttg
     9181 atgaagagcg atcgtatcat ctagctgttt gaagaaagta ccgatttttt gttgttcatc
     9241 ctttacagga accttaacac tgatattctc aatatcagaa ctgttataag aaggcaatgc
     9301 ccctacttgg gagtatcttg gcaaatttat agtttcaaac aatattttac caaaatagat
     9361 atcccaagta ttattaaata aataagccat ggtattgtta tctattaaga aagggcttgt
     9421 gaccaaacgt tttctattaa gcattattgc tgcgccaact ttagcaaata taatcgctgg
     9481 cacatctttt attggcttcc attttttacg ctcaatttgc tcattagata cataatgatt
     9541 tgcatttctc atttcatact cattaccaat gttattcatg tcggaaactt taaaaaatgg
     9601 agtaccttca gaaccacctt gctcggcatc aggaaatcca attcccgatt gagttttacc
     9661 taattctccc aacttacgct cttcccaatc ttccgtaaaa ccaggaaacc ggatttctgg
     9721 cacttttgct ccgttttcag ggaacatttt ttgtaagaat ccttttttcg tttccttcag
     9781 caaatctaac ttacgttgat gaagagcgat agtgtcgtcg agttttttga agaatatgcc
     9841 gattttttgt tgttctttta aatcattggg aatatagaat actgtatctg aaactttttt
     9901 ccagtctgaa cgaggcattt ttgttccaga tgtatcattt gcagcctttt gatatctatc
     9961 agcttgaata agagaataaa taaagtccga gtcgctcttc ttagctctaa aaacccagaa
    10021 atccccaatc gctacgcctg taaaagttgc cttgagccaa ttttttaaat atggacgtaa
    10081 ctttccatat agaatatcat ttggtaaaaa ttcaatccct tttctattat caaatttctg
    10141 gctcacatct ttattcagtc taccttctcc tgccacgata tcttcaaatt caacttttgg
    10201 aagtctactg gagtttgtac ttttattaag tctaacaact aaatcaatta acttacgctc
    10261 ttcccaatct tccgtaaatc ctgggaaacg gatttctggt cgtgtatcat tgctcatgtt
    10321 tgaacacctc cagcgcacct tgtaaccaca tttcgttttc aggtgttgtt tgcagggaag
    10381 agatagcttc taaaagttct ttttctagca cttgctgttc ttgtcgtatc ttcttgatgt
    10441 catttccgac atgaaccata tcaattggtt cttcttcttc gaaagtatct acatagcgtg
    10501 ggatatttaa gttatagtca ttttctttga tttcatcaaa ggttgccaaa tgagcgtatt
    10561 tctcgacatc ttttctttca gcgtaggttt cgaggatttt ttgaatgttt tcttccgata
    10621 gtttattttg atttttccct ttaacaaatt cacgactagc atcaatgaat aatacatctc
    10681 gagtttgacg attcttcttc aacacaatca ccgtcgttgg aatcgatgtc ccaaagaaca
    10741 agttcgcagg cataccgatc acagcataaa tactgccatc ttctagtaat ttttgacgga
    10801 tgaccccttc tgcagcgcca cggaataaaa ctccgtgtgg taagacgatg gccatggtac
    10861 ctgtttcctt caaatgatag aacccatgaa gaagaaacgc aaaatctgct ttggattttg
    10921 gcgctaattt cccataacga ttaaaccgag aatcatctaa gaaagtcgta tctgctgacc
    10981 aattagccga ataaggtggg ttcattacta ctgcatcaaa tgtgtaaggt tcatctgttg
    11041 gccagtcctt attcaacgta tccccattgc ggagattcat ttcttcggcg tcgacaccat
    11101 gtaaaatcaa gttcattttt gctagattgt atgtcgttgt atttagttct tgcccatgat
    11161 atttaacgtt atctgggtgt gttaagtagt tacgtacgtt taacatcaat gaacctgaac
    11221 ccatcgttgg atcaaaaaca ctaaagaatc gtcgttcctt ttgatcgagc gttacgattt
    11281 gcgccatcat atcagaaacc atatgaggtg tgtaaaattc tccggctttc ttccctgctt
    11341 ctgaagcaaa ttgactgatc agaaactcat acgcatcccc gatcacgtcc ccatcgtgtt
    11401 ctaaaacatc tacatcattc aatttcttga tgacttcggt aatcgtcaca tttcgttgtt
    11461 gctcatctgt tcctagtttt tttgattgga gatccacatc atcaaataac ccattaaatt
    11521 gattgtaggt agatgccaat tgcacaaagg ctttgtttag atcgttcaat tggaacgttg
    11581 cttgttttgc ttgatccgct aagacgttga aaagatactc tggtgcaatt gcatatccca
    11641 aaatatcaac gattgtggca atcagatctt cttttgattc ttcatctgaa agtaattcac
    11701 gatacaacat tgtttgtttg cttactgtgt catattcctc taaggattca tctgctaaaa
    11761 gaaccacttg ttccaacaat cgatcggata aatatttata gaaaatcagt cccaataaat
    11821 aatttttata ttctgacgca tccattttac tacggagatt atctgccgca ctaaataatt
    11881 tttgatttaa ttctgcaccc aaaaagatac actcctatct cttatttaaa gttgctatat
    11941 accctcttat tttagcatac ccccttttat aaaaagcaaa aaaaaaagaa agaaacttat
    12001 tcttgctaat aaactacgcg aaaggagtct aacgttgttt gatataactt agactgatcg
    12061 ttaacttatt ttttatattt ctacatagtt atctttagaa ccatttcaga tacatatgaa
    12121 acaattacat atgatttttt cataaaaaaa cagtactgtc tagtagtata aaatatacaa
    12181 atttcttaaa aattttttca ctaaaatcac tacctgaaac agtctaaaac acttatatat
    12241 caacgtttaa tgaaactatc tccgtttcat ttcagataca aataaatata gtcgggctcc
    12301 aaatccgaaa aaaaataccc ccagtagctt atcctaagct actggagatg tataagtacg
    12361 gtcatataac aacggggtag ggtaaataaa tgaaaaagat ggaagaatca ttattcaata
    12421 acttttttaa atacttcgtt gtaatcagtt acctgtccgt tcttaaatac cacagaataa
    12481 tctttttacc cattttcaag taaccgaagt ggaatgtctt taccaacttg ctctgtcact
    12541 gcattactga tacctgttag attagctaat aaatctgata aagattgagc atctgtagaa
    12601 atctctgtat cagtatttat cgtgattcct ttatcttcat ttacaagctt ccaagtaggt
    12661 ggtatagctg tgtatgtttc gatgaatgaa tcaattcttt catctatcgt cttcccagta
    12721 tctacttctg tctcattact cttagatgaa tcgattcttt catccatcgt ctgctccgta
    12781 actgcttctg tctcattatt ctcaccggaa actctcttca cacccaaact tatatattgt
    12841 ccatctttag tgtgcatagt taaaattccc acgcttttac taataaattc actataacca
    12901 gttctttcaa tagagacttc aatgtatttc acactatcat actcaaaaga aaaatagaca
    12961 tgtaaaagca ttttgttgtt tacatctaca taataatcct ttgactctac ttgagacctt
    13021 cctatacgga atacagcttt actgtctgat atctctatac cagcatcctt attattaatc
    13081 tgccaatctc caagtagctc ttctggcaag ctagtagttt cttgtgaaca ctttaccact
    13141 tcactggtag aggtcgaaac attcacctcc gcactgttag aagaattttc caaggaatca
    13201 ggagccttag cacaaccaac aagtctttat atattgtgca gccgatattt tcttcattca
    13261 gtacaccctt gaaatatgca gtgttagata aagcagttcg tcctgatttg ctgaggcacc
    13321 catctcttct tgaacgtact ccttgatttt caaggccgcc cgatactctt tcggaaaacg
    13381 ctgcttcact gaagccagca aaaaatcatc cttctccggc aactcttcct tcttaatcat
    13441 tcgggcaata aaaaactgta aatgcgtcac aaatcttaag taaagcatcg aatctttatc
    13501 aacctttttt tcgggaaaca gatatcctag catagccaag atatccttta gtgctcttac
    13561 cgagagcatg gtttcttcca tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc
    13621 aatgtttcca gcctcctctt ctggcaactg aacattcatt tttttgttga tcaattggat
    13681 agcatagcag ccgatctcaa attcttttgg gtaatacttt tgaatttcaa tcagcaggcg
    13741 attttgaaaa acaatgcctt tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa
    13801 ggtaaaaaat agacttttat tcagcttaaa gtcaaacttg gcgttggcat aatcgataat
    13861 tcgcttgacg caaataatta gatcatccgg agcagcctca aacagtttaa tgtaatcctt
    13921 ttttagagca tcagaaagtg aatctaattt aaatactttt tggattttgt cagccggaac
    13981 caaatcgcct ttctttagtt gaaatccaat ccctttcccc atcaaaatta cttctttgcc
    14041 ttgcttatct aagcaaagaa ttaaactgtt gtttaacgtt ttaattactc tcattagatt
    14101 caccgttctc tctaagctaa gccatttctt gcgattactt tttgatacca aaagaagctt
    14161 tcttttttta ttcgttttaa ttctttaaga ttctcatcgg ttcggtcaac atatataaaa
    14221 ccataacgct tattgaagcc ttgatgggta ctaacaacat caatcgctga ccaaggtaaa
    14281 taaccaaaaa catctacacc atctgttaaa gctgcttgaa tagccagaat atgtttggct
    14341 agataatcaa ttcgatcctg gtcattaatt cgtctatctt cctcaactat gtcgggtgcc
    14401 ccatagccat tttcagtaat taaaataggt aaatcatagc gatcgtacaa tttcctcaat
    14461 gttaatcgta gtccagtagg gtcaactacc cagccatatg gcgttttatc tacataagga
    14521 ttttcagctg cccggtagac accttcttct cccaacatga tctgttgatc ccccgctcta
    14581 gcggaaacat ctgatccgtc atttttgctg gcagcgatgg tggcagttga ataataatta
    14641 atcgcaataa aatcgggttt accggaggat aaaattgcct gatcctctgc ctcaatttta
    14701 ggatataatc cacgatcatt caaatagttt tcaaacaaat aattgtattt tccccgaact
    14761 gctacatcta agaaagacca gcctctcaat gtttcccaat tatgggctgc aattgcatct
    14821 tctggactgg aggtagcttg atacatagcc gttaaattga ttgctggacc gatttttcct
    14881 ttaagattca actgatgata caagtgaatt actctagctt gagctaataa aacatggtga
    14941 ttaatttgaa agagttcttt ttttgaaggc agctctccac ttggtggcaa accaatcgct
    15001 ccaggatgaa ggatcatcgt gttttgctca ttaattgtca gccaatagtt caccttgtca
    15061 ccatactcag taagcaatac ctgtacatag tccacaaaat aatcgatagt tgctcgattt
    15121 aaccaaccgc cctgctcctc tagaaaataa ggtaaatcga aatggtacat ggtgacaatc
    15181 ggttcgattc cctgattcag gcattcatca attacttgat gataaaaaga aattcccttt
    15241 tgattggttt tattattttc aatatccata attcgcgtcc aagcgatgga aaagcgaaaa
    15301 gcttttaagc ctaattcttt aaacagctgt atatcttctt tatagtgatg gtaaaaatcc
    15361 gaggcaactt caaaattagc taccccttct gtatgctgat acttgtcaat aatagacggt
    15421 tgtttcccat cctcctgata ggcaccctct gcctggtagg cactaatgga ggcaccccat
    15481 aaaaacttct ctggaaatgc tgaaagctgt ttgtattgca caaaaatcct ctccttaact
    15541 aatttgcatc aatggttcgt gttcctttaa gcttccgctt gcttcactaa ttcctgcgta
    15601 attttcactg ttggtcacta ccacaatcac tgtatcatcg tagccagcgt ttctaatggc
    15661 agcgaaatcg gcgttgatta ataattgtcc tgctgacacc tgatctcctg cttttacaaa
    15721 tacttcaaag ggttcccctt ttaaattcac ggtgtcgatc cccacatgga tcaataattc
    15781 aataccttct ttagaacgta gaccaatcgc atgtttagat tctaaaatag taatgacttc
    15841 tccctcaaca ggtgaataaa tgctgttttc ttctggatga atggctacgc cctttcccaa
    15901 tagtccctta gaaaagacag catcgttcac ttcagaaata ggacacacgg ttccttttac
    15961 cggattttca acaatgactg cttcggctat tgccgtttgc tctgattcat cagaagtatc
    16021 gtcaaatcca attagataag caatgataaa ggttgcaaca aaggcgatta cacacgtaat
    16081 aattgcgtga acaatattca ttggattttc tccaataaag gctggtaagg ctgctaagcc
    16141 aggggaaaca aaggcatatc tgactaaacc gctcaagcca gcatacaaac cagcaattgc
    16201 accaccaatc atggaagcaa ctaatggttt tttcagcttc aaggttactc catagagtga
    16261 aggctcagta attcccatta aagcagtgat cccagtggac acagctagct gcttcaactc
    16321 tttgttttta gttttcgcgg ctacagcaaa ggtagcacct gattgagcaa tattagaagc
    16381 cagcattcct ggcccattga tcatttcaga acccgtactg ctcaattgca tagtagcgat
    16441 tggcgtcatt gcccaagctg tacccgtaac caccaaaaat ggctgcaaac cacccatcaa
    16501 aaatggaatt aaccaagctg ctttgccgtt aatccattca gcccccattg ctataagatc
    16561 gttcagcaac gttccaaacg gtccaatgac aaccaacgct aaaggcccca taattaaaaa
    16621 taccagcatc ggcttagtaa agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg
    16681 ctctacatag gacatgatcc aaacagtaaa gataatcggt aaaactgatc ccgcataatc
    16741 tgcagctaat accggaattc ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga
    16801 aatatttgga tgaagcaaaa tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa
    16861 ctttatggct gccccatagg ctaatagtac tggcataaaa taaaaggctg catcagcaat
    16921 agtattcaaa atgatgtaag tttgactact gtctgatagt acaccagtca aagtcaagac
    16981 ggccaaaatg gcttttacca tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt
    17041 ggtagaaatg acactaatca atcttgaaat aatactttct tgattgtctg aagaggggcc
    17101 ctttttaatt gaagttccca cttccttttg aatttccttg aaggctgttt ggacttcatt
    17161 gccaatcacc acttggtatt gtccaccgct atccaccaca ctaattacac caagtgtttt
    17221 ttctaaggct ttcgtatcgg ctttcctttt atctttgagt tcaaaacgca gtctggtggc
    17281 acaatgggtt aagctgacca cattttcttt tcccccaacc tgttgcaata catttcttcc
    17341 tacttctctg taatccatta ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc
    17401 acgccccaat caacattgaa gtgttagttt gtcttggtct tgcctgcata atcagtaaca
    17461 tgccaataac gttattttgc tgtaatccaa taatagcaca ttatgatagc gcaatcaaca
    17521 gggaatttaa ttttttatct tttcgtttga ccgtataggg taatataaca gttagatcac
    17581 atcaactagg gtatatctgc gtatacttac tgctaaaata agtatatcta atattaacta
    17641 taccagtaaa accattccta cataaccagc gggaaattcc caaaatcgtg aaagcatcaa
    17701 aaatgcatat aacggatcac acaacttcaa caattctgct cttgggaaaa attgatgaat
    17761 ttggtgagta aaggatggaa taaacagcat tccaacaata taggcaacaa tagtgatact
    17821 tgtttttttg gtaaaaatca gtaagcaata gccaatttca atcattccga gatataaagg
    17881 tatcaataga atgatttggg ctagtattct taaaaattaa
//
LOCUS       DAFBQH010000054        17687 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.056, whole genome shotgun sequence.
ACCESSION   DAFBQH010000054 DAFBQH010000000
VERSION     DAFBQH010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.056"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(136..1716)
                     /locus_tag="LK694_001911"
     CDS             complement(136..1716)
                     /locus_tag="LK694_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBK5052758.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2117..3493)
                     /locus_tag="LK694_001912"
     CDS             complement(2117..3493)
                     /locus_tag="LK694_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBK5052759.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3621..4739)
                     /locus_tag="LK694_001913"
     CDS             complement(3621..4739)
                     /locus_tag="LK694_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK5052760.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4881..5315
                     /locus_tag="LK694_001914"
     CDS             4881..5315
                     /locus_tag="LK694_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5052761.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5393..5926)
                     /locus_tag="LK694_001915"
     CDS             complement(5393..5926)
                     /locus_tag="LK694_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBK5052762.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6020..7198)
                     /gene="mutY"
                     /locus_tag="LK694_001916"
     CDS             complement(6020..7198)
                     /gene="mutY"
                     /locus_tag="LK694_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBK5052763.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7191..7988)
                     /gene="recX"
                     /locus_tag="LK694_001917"
     CDS             complement(7191..7988)
                     /gene="recX"
                     /locus_tag="LK694_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBK5052764.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8095..9477
                     /gene="rlmD"
                     /locus_tag="LK694_001918"
     CDS             8095..9477
                     /gene="rlmD"
                     /locus_tag="LK694_001918"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBK5052765.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9651..10229)
                     /locus_tag="LK694_001919"
     CDS             complement(9651..10229)
                     /locus_tag="LK694_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HBK5052766.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10551..11021
                     /locus_tag="LK694_001920"
     CDS             10551..11021
                     /locus_tag="LK694_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBK5052767.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11129..12355)
                     /locus_tag="LK694_001921"
     CDS             complement(11129..12355)
                     /locus_tag="LK694_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBK5052768.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13109..13309)
                     /locus_tag="LK694_001922"
     CDS             complement(13109..13309)
                     /locus_tag="LK694_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK5052769.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13443..14051)
                     /locus_tag="LK694_001923"
     CDS             complement(13443..14051)
                     /locus_tag="LK694_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBK5052770.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14136..14606)
                     /locus_tag="LK694_001924"
     CDS             complement(14136..14606)
                     /locus_tag="LK694_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBK5052771.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(14654..15568)
                     /locus_tag="LK694_001925"
     CDS             complement(14654..15568)
                     /locus_tag="LK694_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBK5052772.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            15714..16517
                     /locus_tag="LK694_001926"
     CDS             15714..16517
                     /locus_tag="LK694_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBK5052773.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            16695..17642
                     /locus_tag="LK694_001927"
     CDS             16695..17642
                     /locus_tag="LK694_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBK5052774.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         5363 a         3543 c         2911 g         5870 t
ORIGIN      
        1 gacatttcta tgattttaat atagtaaaaa gagccgtcac ggaaaatccg tcacggctct
       61 aaacaaaata tgagaggctg tttttgtttg gctattctta ttacttgatg cagggcagct
      121 tgtgtcaaga cttctttaca tcaaactttt taattttaca ttgggatatt tatccgcaaa
      181 ccagcgttct gcaaattggt tctcgaataa gaacaacggt tggtcgaaac ggtcacgagc
      241 caagatattg cgacttgaac tcatcttctc atccaaatct tctggatcga tccagcgagc
      301 gattttgtgt cccattggag acatgattac ttcggcgtta tactcattta acatacgata
      361 ttggaatact tcgaattgta gctgaccaac agctccaatg atatattctt ccgttagata
      421 tgttttataa agctgaatcg ctccttcttg aactaattga tagattcctt tgtggaagga
      481 tttttgtttc atgacatttt ttgcagtcac tttcataaac agctctggag taaatgaagg
      541 gagttcttca taagcaactt ttagttttcc ttcgaacaat gtatccccaa tctgataatt
      601 gcctgtatca tatacaccga tgatatcgcc tgctactgct ttttcgacgt tttctctcga
      661 atctgccata aattgtgtga cattgcttaa tttcagtttt ttatttgtac gttccaacca
      721 gacatccatt cctcgttcga atgttccaga acagattcgg acaaaagcga ttcggtcacg
      781 atgggctgga ttcatatttg cttggatctt gaatacgaaa cctgaaaatt cttcctcata
      841 aggacttacc atctgatcat cttgtgtttt gtgtccataa ggagctggtg ccaattcaac
      901 gaaagtctct aaaaaagtct gtacaccgaa gttagtcaat gcagaaccaa aaaatactgg
      961 tgtctgatcg ccacgagcaa ttttttcttg attaaatgca tctccagcct ctttgattag
     1021 ttctacttct tctaagactt gttcatataa ggaattttca gttagaggat gattttcggg
     1081 aagcagtcca tcttcatcca attctactaa tcgttcatcc tgataatttt ccggacgata
     1141 aaactctaca cgattatggt agatatcata aagaccttct agccctttac ccataccgat
     1201 tggccaattc atcggataag attcgatatc taatagctct tctaattctt ctaacaattc
     1261 caatggttca cgaccatcac gatctagttt gttgataaaa gtaaatatcg ggatgccacg
     1321 tttttttaca acttggaata atttctttgt ctgtgcttcg atcccttttg cactatcgat
     1381 taccatgacc gcactatcca cggccatcaa agtacgataa gtatcttcag aaaaatcttc
     1441 atgtcctggt gtatccaaga tatttactcg tttcccttga taatcaaact gcataacaga
     1501 actagtaaca gaaatcccac gttgtttctc gatttccatc cagtctgatt tggcaaaatt
     1561 acctgttttt ttgcctttta cggtcccggc ttgacggata gccccaccaa acaatagtaa
     1621 ttgttctgta atcgttgttt tcccagcatc cggatgggaa atgatcgcaa aagttctgcg
     1681 attgtccact tgttgcttta attttggatt attcataacc taactccatt tcttccattc
     1741 aattatttat attttctcta ggcacagatt gtccgatctc ttaaagaaag acaatttcat
     1801 atagggctgt gacaaaagcc gtgtagcagt ctctttttcc gagtaaacgg cgttctagag
     1861 acagcctatc agcaataagc ccacccaagc caaaaatacg aaaagctgtt tttgcttggc
     1921 tgctcttatt acttaatgca ggatagcttt ttcacaacct ttgtcaacta ctacaactga
     1981 atcagtatag catggattca gcgaatgaaa aagccaccaa accataagag tcaaatattt
     2041 ttttgtcagc tgactaaaag aaaagacgtg ttgcttgttt attcaagcaa cacgtctttc
     2101 ataaccaagt ttccaattat cttcttggct cgtcatcttc gaattctttt ctgaagtatc
     2161 ttcgttcttc ttcaggttcc gcttctgttt cttccggatc atgaaaaatg acacggattt
     2221 taaggattcg agaaccttcc atttcttcag aaaccagcgt aatattatcg acatcaaacg
     2281 ataacttttc gccttcatcc ggaatcatgc ctaatgctgt aatcaaataa ccagccatcg
     2341 tatctacatc actcatatgc agatcactgt caaaagcttc attgaactca tcaatcagca
     2401 tacgtccttg gatgatatat tcgtgttcat cgattttttc ataaagattc tcaacttcat
     2461 ctgattcatc atcgatttct ccgacgattt cttctaaaag gtcttctagt gttgctaacc
     2521 caacgacgcc gccatattca tctaataaaa tagccatctg attctgcgtc tttttcattt
     2581 catataacag atcatcaata aagaccgttt caggaacgaa taaaggctcc tgcatgattt
     2641 tcttgatatc taaattatca aagccgaatt tatgggcagc ttttagtaaa ttctttgtat
     2701 gaaggattcc gactacttta tctttgtctt cgttataaac agggattctt gaatagtttt
     2761 cggataaaac ttcattcaca ttttcttcca cagtgtcgtt gatatcaatc ataaatgcat
     2821 ccgttcgtgg gaccatgact tctcgggcaa ctttcgtatc aagagaaaaa accccttgga
     2881 gcatttctaa ttcttcatta ttcaatacgc cttcgttttc aagcatataa cgcatttcat
     2941 ctctggtcat ttttgaatct tcatcatcaa aagtcatcgg cgtgatacgc gccaataaac
     3001 ttgtggaagc agaaagcagc cagacgaagg gcttagctac tacaccaatc aagcgtatca
     3061 gccctgaagt gaactttgcg acttcttctg atttatttaa agcaatacgt ttagggtaca
     3121 attctccaaa aacaatcgat acataggtca aaatagccag cacaatgata tttgcaatat
     3181 tttttgccgc tgtgccgcca cctaataccg gagctaaccg ttccgataaa gtattagcta
     3241 gtgaagcacc agacaaaatg ttgaccaaag tgattcctac ttggattgtc gataggaaat
     3301 tatttggatt ctgcaatatt tttaaaagct tttgagattt cacatctcct tcatcagctt
     3361 tttgctcgat tcgattctta ttgatcgaaa caacagcaat ttcggaagct gctaaaaaag
     3421 cattgatcaa tgtcaaaacg atcaataata aaatctgcgc taacagcgac tgactctcag
     3481 ggtcagcatt cataatcgca ttctctcctt ataaaattaa tttttagttt acgatagaaa
     3541 aaaacgtggc actatagcca cgttaaaatg gtatcacaaa gcaattttct ttgcaataaa
     3601 aacaagtgta ctaaaactac ctacgaggtc gtagcaattt tcttcttgtt ttcattttgc
     3661 ttgtcttctt tgacgatttt gattatatag gaaatgatcg tcttgcagat tgcgtaaaaa
     3721 ggaacaccta agaagatacc aagcaatccg gcaatatttc cagctactag aagaacgata
     3781 atgatcgtta atggatggat tttcagtgat ttcccaatta cattaggata aatgatattc
     3841 ccatccaact gttggacgat tagcacaacc acacaacaaa gtaatgcttt taccggatca
     3901 ttgaagactg ttactagaaa tgcaggtgct agtcctagat aaggtcctaa atatggaatt
     3961 aggtttgtca atcccgcaat gaccccaaat aagaaagcat aatccacacc aattaccata
     4021 tatcctaaaa atgtgaaggt accaacgaat aaacattcga ttgcttgtcc gctaatgtaa
     4081 ttagccaatg ttttattcag ttgccccaat aactccacaa tttggtttct tcttttttct
     4141 gggaaaaact gttggatgtt tggcactagt ttttctccgt ctttcaacat atagaacaag
     4201 atgaatggtg ctgtaatgat tactacggtt gccgaagcga tcgttccaac gatcgaacct
     4261 aaactattgg atactccact aaggaattgc tgaatgattg tcccgtaaga aatgtctaat
     4321 tgctcaaaat atttattgat atctacttcc ttgaacaaag gcaattcggc tacctctttt
     4381 aaccagcttt ctacctgttt gatgaaatca ggcatattag atgccaatga ggaaatttgc
     4441 tgtactagac ttgggatgac actaagtaaa atccaaataa ttgctgcaac taaaaggagc
     4501 agcacaattg cgacagccca tattcttttg atttttgctt tcatcaacag cttgacaata
     4561 ggattcaata aatagtaaag aaagcctgca atcagtacag gtgcaaataa cgtggaaaaa
     4621 aatgtcccga tcggttcgaa aataaagttt atttctgatg aaatcatgat caatgcagct
     4681 atgatcaata attcaagcga ccaaaacatt agtttagact gtctaagttt ttcaaacaat
     4741 agatttcctc ctttctttct tactagtata tattatattc gttaaaaatt cttttattct
     4801 ttttttattt ttgaaaactt gccatacttt ttatttgttt tattttccag tatactatac
     4861 gaaaggaagg tggctagacc atgaaagttg tacaaacaaa agatacaatg agcaatattt
     4921 acttagatgc tgtacggatc aggcatcaag tttttgtagt cgaacagggt gtgcctttgt
     4981 ctcgagaaat agacaaagat gaagcccatt gtatccactt cgtcctgtac tcagataaaa
     5041 aagaaccgca aggaactgta cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc
     5101 ggatggcgat tctttctgaa tacagacacc aagggttagg aaaaatattg attgaagaag
     5161 cagaaaactt tgctaagaat caaggctata atacgatttt attaggggca cagtccacag
     5221 ctgaaacttt ttacgaaaaa ctaggatata cagcttacgg cgatcctttt gaagatgctg
     5281 gcatgcctca tatttatatg aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa
     5341 acagcaagct tttgagaaaa atctctcact ggcttactgt tcttttttgt gtttacttac
     5401 gcgacaattg cttgtaacgg ttataccaaa tatcaatata gccttccgag aacgggccat
     5461 ctccattatt gatccagtca accagaatct tcacattttc ttttaggatg aagtcaatat
     5521 cgttcgggta attcatcatt ttgctgtgca tctcatattc atcgacatcc aatagtcgtt
     5581 tctcgccgtc aggaaatacc ttgatatcca agtcatagtc gatatatttc aaggcctcat
     5641 cgtccagcaa aaaaggtgaa gctagattac aataatagga aacccccttt tctctgatca
     5701 ttgctattac attgaaccag tattttttat gaaaataaac aatcgctggt tcgcgcgtta
     5761 cccatcggcg tccatcagat tcagtgacta atgtgtgatc attcacgccg atcatagagt
     5821 gttcgcttgt ttttaatacc atggtatctc gccacgttcg atgcaatttg ccgtcgtgct
     5881 tgtaactttg gatcgtcaca aactctcctt cttttggaac tcccatagca acccttcctt
     5941 aagcagcatt ttgtgctttg tgtttgggtg ttcattacac cgacatcata attattatat
     6001 caataaaaat gagctttgcc tagaaaggat tcctattgtt tcgatttttc tctaactgat
     6061 ctaccagttt catttggacc ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc
     6121 acttcgaagt tttgttctcg gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa
     6181 aaaggagaac atgccatttc agatgactaa aaatatgagt aacttctcct aaatgtcgtg
     6241 tctgccaaac aaagagttcc tgcttttcaa atgggagatt ttgctcttct tctgctacta
     6301 ggtcatcaaa cagatcgatt tctggctttg tttcccattc ttttttcaat cgctcatact
     6361 cctcttgtgt cacttccatc attggaaatg tccacatatt cgccagcaat ttttggctat
     6421 ctctttcttc aaaataatac gctccagaat gattttgaag agctgcactg atataataaa
     6481 cgtcttttgg ctttgctttt tttgttttta caggaaaact cgtttggatt cctcgtttgt
     6541 tagctaaaca aaaggcttgg atcggacaag tctcacactt tggagaagtt ggtgtacaga
     6601 tagctgaacc tagatccatc attgcttggt tgaattcacc tggatgcttt tcatcaatga
     6661 ttttccgcat cgcttcatca aagattttcc tgctagaagc ttttgcgatg tctgcttcaa
     6721 tgcaaaacaa tctgctgact actcgcatca cgttgccgtc aactgcaggt tctggaagtc
     6781 caaatgcgat acttgcaatc gcgcctgtcg tatatggtcc tattcccttc aatgagctaa
     6841 tttcttcagg cgtttgcggc atttccccat caaattctga catgatctgt ttagcagcag
     6901 cttggatgtt ccgggctctt gagtaataac caaggccctc ccatgctttt aaaagctttt
     6961 cttctggagc atttgctagc tcttcaattg taggaaacca ttccatgaaa cgataaaaat
     7021 aatcgatcac tgtgtctact ctggtttgct gaagcatgat ctcagagatc cagatacgat
     7081 aaggatcacg attatagcgc caaggcaggt ttcttttttc ttgttcatac cattgaatga
     7141 actgatcttg aaattctttt gtttctttat ctgtccattt ttcccattct ttattcactt
     7201 tcctcttcct tttccgtaat gaatcgctgg atcatatcca aatcaaatcc tttttgatat
     7261 aagctttgct ttacttttag atttcttttt ttaggttcaa accttaaatt tcttcgccat
     7321 agtttatctc cttgtttgac taaataatca tattctattt cttgatctgc ttcttgcgga
     7381 agatcagcta tcgcttgttg gatcacttct tgagaaaagc ctttcgtcat caatgtttgt
     7441 tggatttttc tcaataattc tttttggctt tttccgtgat tttttccaaa cgctttttcg
     7501 gctgtctgat gtgcattttg aacttggtct tcaaatgtat attggctgag cgcttgttca
     7561 atgatttcag gtttcaaacc tttttgctgc atctgctgcg ctaatttctt tggtcctttg
     7621 tcacttaagc gcatattcgt gcgcacataa ctttccccat agaccaaatc atcgacaagg
     7681 tccaattctt ttaagcgggc tacgatttta tttcgatctt cttgaaggat ttccttttct
     7741 tttaagtatg ttcggatttc tttttccgat cgcaactgat aacttaagta attcattgct
     7801 aggtgaaaac cgaaatcagc tccgctactc ttcttgatct cttggatagt ttcttcgtct
     7861 aattcccgtc cttttaataa tcgatagcgt actaatacat cttcagatac ttgtaaagct
     7921 tctccttcag ataaatctac gcgatagaaa gggcctttgc ctttactgat tctcgtaacg
     7981 gttaacataa acatcctcct cgaacatata ttctctatca taccacagaa aatccccgta
     8041 atcaggtatt atatgttaga ataaaccaca gaaaaaagaa aaagggagat acttatggca
     8101 gaaacaattg tcaaaactgg acaaacggtt cctttgaaaa tcaagcgttt aggcataaat
     8161 ggtgaaggaa tcggttattt caaacggttg atcatatttg ttccatatgc actaccaaaa
     8221 gaagaagtac tagtgaaaat cacgaaagca actcctcgct atgcagaagg agaattaata
     8281 aaagtcaaaa aaacaagcaa agatcgcgta gtggcaccat gtcctgttta ctatgaatgc
     8341 ggtggctgtc aattacaaca cttagcttat caagcgcaat tggattttaa aaaggatctg
     8401 cttttgcaag cattggaaaa attcaaacca gcaggctttc gatcttatca acttttgccg
     8461 acgatcggga tggatgagcc ttggcattat agaaacaaag cacaatttca gttacgaaaa
     8521 aataagcaga ctcaaaaaat tgaagctggt ttatacgaag caaactctca tcagttagtc
     8581 cctataacag actgcttggt tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt
     8641 caactattga ataaatttga tgcacccatc tatgatgaac ggacaaacag tggaatcttc
     8701 cgcacaatca tggttagaat cggtgttaaa actggggagt tacaagttgt ttttattact
     8761 cgcagtaaaa aattcccgca aaaaaacgca atgatccgtg agatcaatga acgactgccc
     8821 gaagttgttt caatcatgca aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat
     8881 gaaacgatcc acttatgggg aaaagaagcc attgaagaac aaatcaatga agtgactttc
     8941 gatctttctc ctcgtgcctt ctttcaactc aatcctcagc agaccgaatt gctttatggc
     9001 gaaggaatca aagcattaga tgtccagcct aatgaaacga tcgtggatgc ttattgcgga
     9061 gttgggacga tcggcttaag tgtagcaaaa caagccaaag aagtacgggg aatggatacc
     9121 gttcctcaag caatcgctga tgcaaaaaaa aatgcggaaa gattaggata tcacaacact
     9181 cgatatgaag tcgggacagc agagcaagtt ctgcctaaat ggttgaatga aggttttcag
     9241 cctgatggta tcatcgtaga tccaccacgt acgggtttgg ataacgcatt gatccaatca
     9301 ttattgaaat accctccaaa aaaacttgtt tatatctcct gtaatgtttc tacacttgcc
     9361 agagatctgg ttaatttaag taaaaagttc aagattgaat atttacagtc cgtcgacatg
     9421 tttcctcaga ctgcacgttg tgaagttgtc gtgaagatga cgagactcgc tgactaaatg
     9481 aaagaggttg tgaaaaagtc tgccctgtat caagtaataa gaacagccaa acaaaaatag
     9541 ctcttcatgt tttatgaata ctgtacttgt gaccgaagag ttaacaggag gacactattt
     9601 atttaaaaaa agggttgtga catagttttt gtcacagccc tttttgatta ttattggata
     9661 ttcacgtcta tcacttgata aaatgcattt gtcgtatcgc tgatattcca tactgctaaa
     9721 atcacattat atccttttcg atcctgcgga agtgtgatcg tttggttgac tgtttttggt
     9781 ggaacagttg ctttgtcgtc tatttttgtg atcaattcaa aatcactaaa tttcaaaggt
     9841 tgattaggat tccaagttgg tttggtaatg tagtaatccc aagtacttgt ccgatgttgt
     9901 gctgttaaat tccaagtaat atttaatgtt cctggattaa ccatgttttt atgccatctg
     9961 ttagatgtct gttcatctaa aggctcaaag ccgctgactc ctgcactagc aatttttcca
    10021 tcaatgaatg tatttttggt agcttcgata ctttgcggtt cccattgtgc tcttccgaca
    10081 ttttgattta agtttcctgc tgctgatgtt cctaggtatg cacggcttcc tggagaagta
    10141 atataaccat gcgcaaaacc tggaatgcta aaaccgaaca accctaaact aactaaccca
    10201 ataaacaata acgtactaat ctttttcatg tagaaacctc cttcatttga taaccacatt
    10261 gtaaacgctt tattgtatta acacaaacat tatgctttta taattttatg ttgaagtaaa
    10321 aaaggtgaaa aaagtaagat gcagattata acagaagtag acgagaaaag tacaaataga
    10381 aaacatgaga atcaaatcat atgatctttt tcaaaaacat cattcatata aacaaatgtt
    10441 caacttttta ataacaacaa ttatcaattt ttttcttact tcacaaacta aatacaggtc
    10501 ttgctgtgag ccttcatggt aaactactat tatagaaagg agttagtaaa atgaagatga
    10561 taaaagatat tcgtttttgg gtttgtgtta taggaattgt catattaggc tttttatcag
    10621 ggttgcttag cggaaatccc ggagaatatt actattcttt gcaacttcca ccatttgcac
    10681 cacctagctg gatatttggg ccgatgtgga ccttactgta tatattgatg ggtatttctc
    10741 tttatttgtt acttaatcac aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg
    10801 tgattcagtt catttttaat tttatatggt ctgctctatt ttttaattta agaaatattt
    10861 tcattgcagc aatcgatatt acacttttag ttattttctt gtcagtgctt atgtatcagt
    10921 tgtggctaca tcatcgcctg gcaatgtggc tcatgattcc ttattattta tgggtactat
    10981 tcgcaactct attgaattat tcaatttatt ttttaaacta agcgctaaaa agagacgtcc
    11041 tgtaatttaa cacatagaaa aagtgccacc taagttagat tttttgtcta atttaggtgg
    11101 cactctttat taaccaaact atttttttct agtacccact tgttgttcca tactcattat
    11161 tctggtcata accataacca ttttgttcct gtccagcgta aggatcataa ctttgagtat
    11221 cttgacttgg tgtatagtac tgacttgtat cttctgtttg ctgataatag gtattcggat
    11281 cttggtactg atcatatgtg ttttctgaat aggaagaata atcggtgtac ccttcactat
    11341 tcgatccatc accgtttgtc gaatcgccat cttccgaatc atcaaagaac atcattgaag
    11401 cattctcatt tttcaaatta ggtaatgtat cgcttgtttt tatatttaat tgtttcttca
    11461 gttcattttg gatcgaaaga agatcatttt ttcctaaaat ttgatagtag atatcattga
    11521 tcatttgtcc ttcgccttgc aactggtctt gtttgattgt ttcaaacgca ggggtgtagt
    11581 tcgtcgctac atcaagcatg tcatcccaat ctaagtccgt cgtcacattg ttctcaactg
    11641 cttttaaaat ttttctataa ttgcttacgc catctaaact taaaacttta ttgacgattt
    11701 tcgtcactac ttcccgctga cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc
    11761 gcattcgaga ataggctaaa cctttttctc cattcaatgt ttgttcacct actggtacat
    11821 ggacaccatc caaagtaaag tcaattttat tattcacttt gatgccaccg accgcattga
    11881 tcaaatcttc catgccatcc aaattgatcg ttacataatg atcaatagga atatcgagca
    11941 gttgctcaat tgaatccatg gccatttcga caccaccaaa tgcataggca tgatttaatt
    12001 tatctaccgt tccataacct acgatattcg tataaatatc tcggtccaaa ctgatgatcg
    12061 ttgatttttt ttgtttcgga ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt
    12121 ccgttcttcc taatccaccg gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt
    12181 catcaatgct tactctctca gaacgtttgg aggatattcg attagagttt ttagaaattt
    12241 tgtttactgt ttggctcgct tctcccataa atctgattcc gtatgctgat aatccggcaa
    12301 tcagcaaaac aaatacccct aacaaggtta atagaacttt ttgccatcgc ttcataatac
    12361 acctctgttt cacattttct aaaatattgt ctcatataac tgttcaatca gcaaattcac
    12421 tttttaatca taagaaaact ttaaaaaaca tttttgtgta acgattacta ttttttaaaa
    12481 tttctcaaaa ttcttaacta tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac
    12541 gcagttcgca atcgttttat ctttttttac gtattttttc cagaaaaagc gaacaacaag
    12601 gtattttcca aataatcgtg cagtaaaatg atcgcacata cctttcccat ctttttaaca
    12661 gtctttgcct accgttcaga ttatcattcg atttcacgaa aatttcttcc tatgactggt
    12721 ccttttgtta aacccatctt ctattcgttt tatttccagc tcactgcatg gctcttaaaa
    12781 tgactcgaac tagtgatttc attaccatca tcttttaatg gatagatatg ctcctcttca
    12841 aagtaaaacg attttctttt aaagtaatag aattgacatg gtaatgagta tgtatactag
    12901 tggtttcaca aatggtcttt tattcaacat ccaaaaactg aaatttcttt aaaatccatt
    12961 actaacgaag aaaatgttcc ttgcttgata attagttagg acatgtcaag tgaacaaatg
    13021 catttactta gccaaaaaat ctaaaaactt cataaataga caaaaagaca cgactctcta
    13081 agaagtcgtg tctttattca acaaattatt ataatttagt cacttcagca gcttgaggtc
    13141 cgcgtgcacc ttcaacaatc gtgaattcta cttcttgacc ttcttctagg ctcttgaagc
    13201 cgtctccttg aattgctgag aaatgaacaa atacgtcatc tccgccgtct actgtaataa
    13261 acccaaatcc tttttcattg ttgaaccatt taaccgttcc gtgttccata aatgtatagc
    13321 ctccaaaaaa gttcttgcaa gtaaccttgc aagaacatta tacccaattt agaaaacgct
    13381 agcaagcttt cgctctttta ttttccaaat tatttttaag actgatatga cggaggaaat
    13441 ggttattttg ctgcttcgaa acgtttgttt acttcatccc aattaataac attccagaat
    13501 gctgcaatat aatctggtcg gacattttta tatttcaaat aataagcatg ttcccaaacg
    13561 tccaaaccta gaattggtgt ctttccttcc atcaatggag aatcttgatt tgcagtagag
    13621 gtaatagcta attttccatt ttccattaca agccaagccc atccagaacc aaatcgtcca
    13681 gcggctgctt ttttgaattc ttctttaaaa gaagaaaaat caccaaaagc ttcattaatc
    13741 gcttctttta tttctcctgt aggttcgcca ccagcatttg gtgccatgat ttcccagaaa
    13801 aatgaatggt tagcatgtcc gccaccattg ttacgtacag ctgtcttgat atctgttgga
    13861 atagcgtcca tatcagataa taattcttct attgtttttt cgcctaattc tgggtatttc
    13921 tcaattgctg catttaaatt cgtcacataa gtattgtgat gcttatcatg atgcagatgc
    13981 atcgtttctt cgtcaatata aggttccaat gcgtcatatg cataaggtag atctggtaat
    14041 gtgtaagtca taaaaaaatt cctcctcaat gttttcttta taatgtctac attacaaaga
    14101 gtaacaaaga gaaggaaatt cttcaattgt tatgcctagt cgtctttttc actttgacct
    14161 acttcttctt caaaaacctc agtttcttta aaaataaaca acttgctgaa aatatagttc
    14221 gctaggataa caataatttg agtgatgatt ttcgccacat actcattgct gttcataaca
    14281 ttgatcaaca ataccattgc ccccatgtcc aatccgtaag aaaggatacg gtagaagaag
    14341 aacttaccgc attcaataag tgcatgtgta aaattcggtg attttgaatg gaatacccaa
    14401 agtttgttcg ttacaaaagc aaacaataca gaacaaaacc acgaaataga attggataag
    14461 gccatactta aatcaaaata ttggcgtgcg agagcaaaac taacaaaatt tacaatcgta
    14521 gccaaaccac caaaaaagag atagactagt atctcccaat atccttttgc ttctaaatat
    14581 ccttttaact gtcgatactg cttcatgagc caacctacct tattaaaaat tcttatttat
    14641 catactctat atttcaataa ctttcatctt tatttaaaaa aattaatttt ttttgatcct
    14701 ttgcctttgt ctctgcttgg atcgtcacat gtgtaatacc atattttaat tttaataaag
    14761 aaattatacg aggatatatc ttttcaatct cgcttagtag ctgatcttca aaatcaatat
    14821 gtccgtcgaa aataattatc ttttcgctat actgccaaat atggacatga tgaacattct
    14881 ttaccccatc gattgcttca atttcttgtt ccattttttc atagtcaata gcaggtgcac
    14941 tttgtatcaa aatagcggca gcttttttga tcaccattat cgcttctatc aatatatata
    15001 tcgaaatgat cagtgtaata actggatcga tccagtagat atcgaaaaaa cggatcaaaa
    15061 ttgctccgat aatcacactt accgaagaca gtgtatcact taacaaatgc aaataactag
    15121 attttatatt caaactttca tctgcttctt gtcgtaataa taatactgag aacaaattcg
    15181 cgagcaatcc aataactgct actgttagca tcaaatgact atttatcttt tctggtgaag
    15241 tgaaccgctt gaatgcttca acggttaaaa acaaggataa tatgatcaat atgaatgagt
    15301 tgatgaaagc actcaatatt tctaaacgct ggtagccata cgtacgtttt ttatttgctt
    15361 cttttcgggc cattttttgt gccccgtaag aaaaaataat tgccaatgta tcactaaggt
    15421 tatggaaagc atctgaaaca agagacaaac tccctgacat aatccctcca ataaattctg
    15481 ataaagtgat caccgcattc aaaaccacta ctccaaatag tttttttgat gatctctgtg
    15541 gctgtgtagg cactattcct tcctccattt tatttatatt ggatagtctt tagacaagaa
    15601 taacattttt ttgatatttc accaatcatt cagatgtttc cctaaagcac ttgtatttca
    15661 cttgatactt cgctagaatt gcaagtgatg acactactag atgaggagta cttatgacaa
    15721 caaaacaatt aattatcagt tcctttatac gattcaaaga cctaaaggag acgagaaatg
    15781 tcccattttg gaaagccatt gtctatcttt tagttttgag tataattatg gctttgccaa
    15841 tctcttttca agtttttcaa gtcttaaaca acattcaatt ggatgggcaa aagattgcta
    15901 ctcgcattcc tgattttaca atccaaaacg ggcagcttca aaccgaagaa aaagaaggat
    15961 tcatttacca aacaaactcc attatcttca catttgatcc agagggaaaa cgaacagaac
    16021 aggacatctc tactgatttg atggggaatt ttgttagtgt cgggatgtta aaaaacaaac
    16081 ttatcatagc tcttcctaat acaggcacga cttctgcttt gttgaacaat aaccaattag
    16141 aacttcccta caccaatgaa tctttaaaaa acttaacagg taaggagctc cgttcttttt
    16201 taagtaaagc atctatacca ctttggataa aagtcctgac atttatattt tctatttatc
    16261 ctagttttct gaatctgctg atcactttgc tatttaccaa cattgctgct tttctctatg
    16321 cacgctttcg actaacaaaa gctacttttc ttgattgttt aaaaacattg atctattcaa
    16381 tttcattgcc tactataatt gcaacaatct tgatggtctt tcttccttct tttgacacaa
    16441 gcgccttcat tgcttttgca ggaatcttta tttttgctca agctgtcaaa ggatgggcaa
    16501 aaatatcgat caattaagaa ataaagacaa ctcgttattt ctacttcaat agaccctttt
    16561 agaggttgtg acattttttt gtcacagccc cttttttatt tgaaaaatat ttctttctca
    16621 aaaaacaagc aaggcgcttc aaagttcttc ttgtatgcgc tacacttaga gtaaagaaag
    16681 gcgggaaatt aactatgtca gaaagaatca tttatgcaga tcgtccattt cgtgatgaat
    16741 ttatcgaaga aatccatact atagcgcctg gttatatctt taaaacagaa ttacaaccag
    16801 aggatctaca aaatgtagag atcagtttgg gctggaactc agactaccaa gaaaaactat
    16861 tagcctcttc taccttgaaa tgggtccaat ctatctcagc tggtgttgat cagctacccc
    16921 ttgagacttt ttcaaagaaa catattttac tttcaaatgg tagtggcatc cacagtgaat
    16981 cgattacgga gcatgtctta ggaatcatcc ttgggtattc tcgtggtctt tttcaagcac
    17041 aaaaagcaca gagtgacatg aaatggttag gttcatcggt ccattaccaa tctatccaag
    17101 gaaaaaaaat actaatcatt ggaactggcc atattggaaa aatgattgca aaaaaatgca
    17161 aagcattaga catgaactgc tatggaatca atacatccgg tcatccagca gaaggaatgg
    17221 aaaagacttt ttctttaaat caactaaagg aagttcttcc tgaagtagat ttcgtagtca
    17281 atatcttacc tctaacggag gaaacaactg ggctctttga tcagcatatg tttgaacact
    17341 ttgatccaaa atctgtattc atcaatgttg gccgtggtgc ttctgttaag acacaagatc
    17401 tggtgcaggc tttaaataac cagcaacttt cctttgcagc attagacgtt tttgaggaag
    17461 agcctcttcc acaagatcat cctctttgga aaatggacaa tgtactgatt acttcccaca
    17521 ttgcaggttt gacacctgat tttcaaaaga aattaatggc tattttttta accaatctga
    17581 aaagctactt tgccacccat gagcttcaaa ccaatcaagt aaaattgtct gcaggatatt
    17641 aatagctgaa aacaatattt tcaaaagaac aaagagaagg tgacaaa
//
LOCUS       DAFBQH010000055        17516 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.057, whole genome shotgun sequence.
ACCESSION   DAFBQH010000055 DAFBQH010000000
VERSION     DAFBQH010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.057"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            100..2427
                     /locus_tag="LK694_001928"
     CDS             100..2427
                     /locus_tag="LK694_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK5052775.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2553..2882
                     /locus_tag="LK694_001929"
     CDS             2553..2882
                     /locus_tag="LK694_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBK5052776.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2922..3926
                     /locus_tag="LK694_001930"
     CDS             2922..3926
                     /locus_tag="LK694_001930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBK5052777.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3923..5352
                     /locus_tag="LK694_001931"
                     /pseudo
     CDS             3923..5352
                     /locus_tag="LK694_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            5558..6730
                     /locus_tag="LK694_001932"
     CDS             5558..6730
                     /locus_tag="LK694_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK5052778.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6892..7347)
                     /locus_tag="LK694_001933"
     CDS             complement(6892..7347)
                     /locus_tag="LK694_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052779.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            7936..8358
                     /locus_tag="LK694_001934"
     CDS             7936..8358
                     /locus_tag="LK694_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5052780.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(8524..9717)
                     /locus_tag="LK694_001935"
     CDS             complement(8524..9717)
                     /locus_tag="LK694_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5052781.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            9941..10318
                     /locus_tag="LK694_001936"
     CDS             9941..10318
                     /locus_tag="LK694_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK5052782.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            10306..10644
                     /locus_tag="LK694_001937"
     CDS             10306..10644
                     /locus_tag="LK694_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK5052783.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            10778..11221
                     /locus_tag="LK694_001938"
     CDS             10778..11221
                     /locus_tag="LK694_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HBK5052784.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            11483..12298
                     /locus_tag="LK694_001939"
     CDS             11483..12298
                     /locus_tag="LK694_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5052785.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            12308..12970
                     /locus_tag="LK694_001940"
     CDS             12308..12970
                     /locus_tag="LK694_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBK5052786.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            13175..14839
                     /gene="alsS"
                     /locus_tag="LK694_001941"
     CDS             13175..14839
                     /gene="alsS"
                     /locus_tag="LK694_001941"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBK5052787.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            14852..15562
                     /gene="budA"
                     /locus_tag="LK694_001942"
     CDS             14852..15562
                     /gene="budA"
                     /locus_tag="LK694_001942"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBK5052788.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            15692..16189
                     /locus_tag="LK694_001943"
     CDS             15692..16189
                     /locus_tag="LK694_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBK5052789.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            16374..16634
                     /locus_tag="LK694_001944"
     CDS             16374..16634
                     /locus_tag="LK694_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052790.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(16996..17235)
                     /locus_tag="LK694_001945"
     CDS             complement(16996..17235)
                     /locus_tag="LK694_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052791.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT         5944 a         3045 c         3473 g         5054 t
ORIGIN      
        1 aaaaaagccc caaactttct atacatcaat aaaataataa aaattaaatt tagaataacg
       61 tgtaaaggat tatttgatga ttttttattg agaggaggga tgaaaatgat taagaaaact
      121 caaaataaaa tccaacgtat atgtcaaaaa cattttgttt ctagactcaa agctgtatct
      181 aaaagaaaat acttacatcc cattatgatt tcgatcatct tgatttttac tttcttaggg
      241 agcttatcaa gtatttatgt aaacgtttat acagaaacgt caaattttgc taaccaaacg
      301 acagaggaga caaatagtcc accctattct gaaatacagt ccatagaaag caccaatcag
      361 tctagcaaca acgagacaac ctcaaccaca tcctcgactc aactgattga tccgacagaa
      421 aaagaaatca cagcagcaaa agcagcaggg gagaaagctt attcagaaac aggtagaagc
      481 cagacattta gtactgttgc ggccactgcg aatttcaacg gacataccat accagatgga
      541 tatacagtga tatatgccaa agatggagtc attgttgcgg ttaacggatc gatgagtgcg
      601 gataaaactg cgttacaagg cgtaaccaaa gcaatcgctt ttattgataa ccgtccagat
      661 atccataatc tgtttattgg ctccacagta aatggtacta ctatcacatt cccaaaaagt
      721 atctatatgg gggtggctac cgctggaaat acgatcgatt cagttacacc caatgcggat
      781 aaaagttatt cttttacacc ctcaacatgg ggagggcttc ctgccggagg aggtatgagt
      841 actttttcaa tcggtataca gttagcaaat gatattccat tcacggggta ctcgatcaaa
      901 aatgggcaga tcgttgatgg taagggaaat gtttcaaatt ttccaaccta tcaagcagca
      961 acggttcagg tcaatgcgaa tcggacagga actttttctt atacagtcaa tggtggagca
     1021 gcaaaaatcg gaacgtacgg gactacatta tctggaatcc atgtgggtga caaagtggtt
     1081 atcactccaa cagcagtaac gggctattct tttgcacaaa caaacggttc tttcacagcc
     1141 ctagaaagta acgtcgatac ggtgacctat acaggcaaac aaacaccaat cactattaag
     1201 tatcaagact attttggaca gatgattgct ccttctaaaa taatgaatct tacttacgga
     1261 agtgctgcac aagacctgac gactaatacg cctactatca acggatatac ctttacagct
     1321 gtttcagcga cagaaacaaa gaaccaatca gcaatgagtg ttttagctag tttagataca
     1381 aatggaaatg taatcgtaaa ggacgcaaat ggaaaacaga tttctgaagt catcctatat
     1441 tataagacaa atgcaacagt atcaatcaat gcaaatggca gtaagtacta tgatggtctt
     1501 tctgttgtac cgatcgttaa atatgccttt aatgataaaa cagaatcaac atccctttta
     1561 aacaatgatc tcagtattga tcaaatcaca tggaatccaa ctgatttcaa agcgatgaat
     1621 gaaaagggag ttgaagtaac tgcgcctact gaaattggaa cctatacgtc ttggcaattg
     1681 acaaaaagcg gactagctaa actagctgcc agaacaaact atttatttac tatcgtgcag
     1741 acatctgatg tctatacgat taagcaaatc agcggtgcgg tagagctcag tggcagtaag
     1801 acatatgatg gaaaagcagg tgtcccgtca atccatgtca agctagcaga gggtgtcact
     1861 agttcgctta ctccagtaca agtggcactt agcagtatgg attacacagc agatgcacaa
     1921 tcagcaaaaa atatggttaa tgctggcagc tatacaatta aactaacgaa tagcggaata
     1981 gacaaagtca aagcagcaaa tcccaagttg tcatttactg atatagccaa cacatcagga
     2041 acttatacca tctataaagc taacgcagtg attactgtcg atgacgctgc ctttaattat
     2101 gatgcccaaa gtcactcgat ttcggttgga aatctccacg ttaccggtgt tgtcttaggc
     2161 gaatcattgg attataccct aacagttaac agccggacag atattggatc acaaatagta
     2221 acgattagtt taggcgaagg aagtgtaaac aataattata cgattatcgc aacagatacc
     2281 gctaagttga cgatcaatcc gactctcttg cttccttcga caggtgggat aggtctactg
     2341 ccctttgtct ttttaggact gatctttatt ttttctggct tcttttattt tatccatcgt
     2401 aagaaaaaag caggtgaaca gcgatgaaaa aaacaaaaaa tgtacgtgtg gatatatttg
     2461 ctgttctctt ttttatccta ctggtctttt ttataggttc tccgactgtg tcagctagag
     2521 aatcacctat tacatttact ttgcataatc gaatgttcga aagcgataag atgccaagta
     2581 tcagtcaaaa cgacggaata aatatcccgg ataactttca aggagtaaaa gggttgaacg
     2641 gcgttacttt cgctatatat gatgtctcgg atgaatttta caagctgcgg tcagaaggaa
     2701 gtagtgtgga agacgcacaa agaaaactag cacaaaaaag tgatagtgaa aaaatactgg
     2761 cagaaaatgt gacaaaaaca gtagacgagg aagagggcat tgcttctttt agtgcatcgg
     2821 acaaagatga gcaaggtcgt gacgctgttt accgatttgc ggaaataaag acttcagatt
     2881 aagtaaaaga aaaatcagca ccatttgtcg ttgtgcttcc tgtgacagat tcatctaatc
     2941 ataaattgac gaccatccac ttgtatccaa agagcgaaca aaaaatacct gctgcactca
     3001 cattaacaaa aacagtagag aataagcaga cagattttgc agatggggat aaagttcctt
     3061 atctcatcac cgtaacaatt cctgagaaca tcaacgagat aggaacctat acgatcaaag
     3121 atacagctga tcctcagtta tggctagaac cagaaacgat cgattttttg attggtggag
     3181 acaagattca tacatttcac acacaaaaaa caaaacatgg ttttgtctta tcagattctg
     3241 gaaaagatct tagttcgttt gctgggaaaa aattaacgat ccgttaccaa atgacattga
     3301 aagaaaacag tgaaaaaacg acatttgata acgatgtacg tttgacgact gacaatcaaa
     3361 cagtagaaac acatcttgcc atccaaacag gaggcaaaca atttgtcaaa gtggatcggc
     3421 aaacgaaaca aaaactagca gatgctcaat ttctagtcaa aaaaaatgac caatacttag
     3481 cgcaagaaaa cgggataaat cattgggtag ccaaagatga ttcaagagca acgattttta
     3541 cttccagtaa agatggaaca ttttctgtcc atggattgtc ttttggaagt tacgaactag
     3601 tggagattaa accgcctaaa ggatatatat tgagtcaatc aaccattctc tttgaagttg
     3661 aaaaaggcag ttatgatccg cttcatccta taccattgga aatcatcaac gaaccaaaag
     3721 caaatccgac gaataatgga aaaacgccag ttcagatgca gacaaatggt ccatcaaatg
     3781 aaaaaaatgg aggaagcttt ccaaaaacaa gcgaagagat gcttggaggt ttctcaattt
     3841 tagggttgtt gctagtcctt tcaacaggga cagcttggtt ttataaaaaa caacaaaaag
     3901 gaaacaatag gagggaaata aaatgaaaaa catcaaaaca aaaatatgga cgtttttagg
     3961 aactgctatc atgctgcttc ctttcgttct cggcctagga actgcagagg tttctaggcc
     4021 gtatcaccta caccagaaaa tgttacggtt aatttgcata aattgaaatt cacttcagca
     4081 ccagaaaatc aaataaacaa cggaacggaa ttgacatttc cgaatagcga accgttgaac
     4141 ggcgttgaat tcaacgtata tgacatcact gctacgtact atccaagtaa agacacagca
     4201 gttccagcag acgctacacc cttcgcaagt gtaacgactt ctggtgaagg attagctaac
     4261 ctgactcttc caggtaaatc tgatgggaaa gatgctgtat acgtctttgt agaaacacca
     4321 aagcccggtg tcgaaacgtc gccaaatatc gtgttaagct taccctttta taacccgaat
     4381 tcatcagacc ctgacaaacc tttagacacg attcacctat atgctaaaaa tgtcatcaaa
     4441 gaaagcaata tcaaaatcca gaaagctggg aacttttcag gggtagataa gttagcaggg
     4501 gcgcaattcc agatcaaaaa ttcagagaat caattcgtga ccggttttga tgaaaacact
     4561 ggtgtcgctc tctatgatcc tgctgagtca gcgttgacat tcacaactga tactgaagga
     4621 tccatcagtg tcaatggatt aatagacggc aattatactt tagttgaaac aaaagcacca
     4681 gatggttatc aattaaccaa tccacaaaaa gaaactactt ttatagtaaa aaacggaaaa
     4741 ttgcaacaaa caaccggtat catccatggc gcagatgaag ccaatcttgg gaatccaaac
     4801 ggtgtagtca atcgggaaaa tacaactacg gataacacga ttactgtaga aaatgtccaa
     4861 aactacggtg atttccactt catcaaacaa gatgtgaaca cgcaagaaaa attagccgat
     4921 gcagaatttt ctattctaag agcaaaagat ccaactgtta aattaatgaa aaaaatagca
     4981 aatcttgcct caggagaata cccatatgct tggtcagatg aagtcacagg ttctgactgg
     5041 gaagaagtga aattgatttc tgattcagaa gggaaatttt caatcgaagg gctgaaacaa
     5101 ggggcatact gccttcaaga aacaaaagca ccagaaggct acgcattacc taagaatgaa
     5161 gctgcgtaca ctgaatttac agtagatgat gatccaacaa cttctgatga aacaacgatc
     5221 gataaccaac ctaaaggaat cttgccttca actggtggta tggggatcat cgtattcatc
     5281 ttggtaggta ctgctttagt tggaggagca gtgatctact tcaaaaaacg ccatcaagaa
     5341 acagaagctt aaactattgg aaacaaaaat catacgataa ataaacgata gtgattcaaa
     5401 tggggagaaa ggttgtgaca agtggtcggt ctcttggtat tcgctcaaca aacggcaaaa
     5461 taggaggaac tattttgccg tttgttgagc gaatactcag gtcaaggcgc tgatttcaca
     5521 acctcttcat atacatatta gaaaataggt ggacatgatg aataaaagca gaataagaat
     5581 actggacatc ttcatggcaa tcattttagt cgttgggatc gggatctttt cttatccttt
     5641 tgtagaagat tcactgaatg attttcttgc ccagcaaatg atcatccatt atcaaaagca
     5701 agcatcgaat aagaattcag aggaaataaa aaaacagcag gaaaaaatga caaagaaaaa
     5761 ccaacagttg gcagagcaaa acgtatcacc agggataggc agtttcaacc aagcagttga
     5821 tgcaaaagca ttaaaagatt tacctagtaa tgcctttttt atggaacata tgcttggagt
     5881 catcgaaatc ccaaaaatca atgtcagctt acctatattc gatcagacaa cggaaatatt
     5941 tttgcaaaaa ggaacgtcat tattggaaga aagttcttat ccgactggcg ggaaaaacac
     6001 ccatgccgtt ctttctgggc atcgtggact cccagaagca aaattattta cagatttgcc
     6061 taagttgaaa aaaggagacc agtttttcat ccagatcaat ggaaagacat tggcgtatca
     6121 agtagaaaaa atccaagtgg tcttacctga tgaagtagat tctttgggta tccaaaaagg
     6181 cagagatctc gtgactttgc ttacctgtac tccttatatg gtcaatacac atcgactgct
     6241 tgtgacgggg catcgtattc cttatcaagc aaaagaggca aaaaaagcga tccaaggaat
     6301 tgatcaatgg aaaaaatgga aattttttat ttggtttatc ggtatactac ttggatcaat
     6361 cggcttagtt tggctactca tcgcttattt agattctttg gccatcgcta agcgaaacta
     6421 tcctttatct ttttatgtaa agaacacaaa cggacgacca atcgaaggaa tggtcttttc
     6481 tgtaaaaacg ctcaacggca aacactatat tactagagaa aaagttccat ttgtgaaagc
     6541 ttctgatgaa tacggactag tgagattttc tgatttaaaa ggcaggaact atcgcttgca
     6601 acatgaggag ctcctgctta aaatccatgt aaaacataaa cattcgaaac agttttcaat
     6661 gaaattgaaa aaaggaagat ataaactgcg aaaagaaaaa gaggtgtact atttgattga
     6721 gaaagaatga atgaagatgt ccaaaaaagc agagggaaca gactaaatga aaaaagagag
     6781 aagctgacga aatggacgtg taaactgcta tttttggttt actatctgga acgccagctt
     6841 ctattttttt atgtttcaga agaattttat tattatagta taacttttga attagtttcc
     6901 ataacatttg gagcaagggg ttaaaccacg agcttgagct tcgcttaaag tggcaggggt
     6961 atacgtacca tttccacatt tatgtgtatg gtattttgaa ccggtcggtg tgacatagac
     7021 agtttgttca ttattttgct caggagctgc ggcttgtgcc tgctgagagg cctgttcagc
     7081 tgctgcttgc tgtcgcgcct gttctgcttg ttgctgtgcg attgcttctt gttgcgcttg
     7141 ttctgcttgt tgtcgtgcaa tttcttcttg ctttttccgt tcttcttctg cttcttttgc
     7201 tttgattgtg gaatcaattg cagcaagacg gcttgtcaac tcttgtttgt tttcaggtag
     7261 cgcttggata gctgctgaag cagttgcata gttttctttt gtaggatttg cttcagcttg
     7321 tgacaaagca gattccgctt ctgtcacttt tttttctagt gcagcttttt ccgctttttg
     7381 tttctcttct tcttttttct tttcaatttg ttcttgttct ttcgatgaat ctgccgtact
     7441 ttcggagatc gtggtactgc tgctggaagc ctgagctgaa cttgtgcttt gagtagtaga
     7501 cgtagtagca ccacacccac tcaacaaact tcctgcgacc aaaaacaatg caactaattt
     7561 tttcactaaa tttcctcctt aagtaaaaaa caaccgtttt caataaatgt ccttttcaat
     7621 tgtttactta cttgttataa taattgtgtt atgaatagtg attgaggaaa cttaatctaa
     7681 tcccgttttg atacacggga ttttttgcta tagttctgtt attaagtatg caataataaa
     7741 atatgtacgt tattttatta ttagattatt gacatttata taataaacga ttaaatgatt
     7801 catatcaata gaatacttag gaaaaaaaga gaaataataa caggtttatg cactattgac
     7861 ggtgtgacaa tatgatattt gtatccgttt ttttcttgta tgtagtatag taaagtagat
     7921 acaggaggag aaaatatgca gactttttta acaaaagttt ctcaaaagaa aattaaaatg
     7981 attcatttac tgcttgaaac agaacgttgg tgttcaatag aagaactaca aaacgaattg
     8041 aaggtatcaa gtaagtctat cttgaattat ctaacagatt tagaagaatt gttccaaaaa
     8101 tatcctgata aggtcatttt gaagaatgag aataatcggc gcttttctat ggaaaagcag
     8161 gagaacttcc caatctacat tatttatctt catttctaca gaaaatcata taattatcat
     8221 ttgattgaat tcatgtatca acatcctgag aaaaatttgg aagattatgc agacgctcaa
     8281 tatacgagtg tatccacagt ttttcgttat gctaagttat tagtaaagta ctttgaaatg
     8341 acggacgtca aagaatagta gccgccagat agaaggaaat tagcagttga aaaagctgcc
     8401 ctgcatcaag taatacgacc agccaaacgg aaacagtgtc tcatgttttt gatttggctg
     8461 gtcttattac cgcagatgca agcttttgcc acacctcttt cttgtcattt acagactcaa
     8521 ctatcaatgc tgcaagcttt cccttaatgc tgtaacacca gtaagagttg tcaagaccag
     8581 cagactgact aggtaaatac cgctcaaaaa caagattacg gcagttaaac tataatgatc
     8641 catcaagaaa ccgatgacga tagaagaaaa accaccaaca gctcgtccaa ggttcataat
     8701 gaaattattc gccgtcgcgc ggatttctgt tggatacaaa ctactaatca ctgcgccata
     8761 tccaccatac atgccattga taaagtagcc gactatcaca gcggctgcta ccaaactcca
     8821 ctggctatgg gctaacacaa gtagatagac agataaggat gaacaaatta aaaacaaaga
     8881 aaaggctagt ttaggcccca atttatccat gattatgcca aaagtcatca tccccaaaga
     8941 catgcctaaa atagtactga ccatccacaa tgaagaccca gaaaccgaaa gccctaatcg
     9001 attttgcata attgaaggca gccaattcat taacccaaaa tagccggcaa tttggacagt
     9061 taccatgata ctaaggccac atgtttgcca agcaagatgt tttgattcaa acagtctgct
     9121 aagtttggtt ttcttttcgg cagattgtac agatttgaat tcctttggtt cagtcaagcc
     9181 gcgacgaatg aacaagacca tcaacaccgg cagtacacct acgacataca acatcttcca
     9241 accaaacgtt ggaatgatca cagcagctaa aatcgcagcc aaagccgcac cgacctgcgc
     9301 accaatacca gcaaccgaag aagctcgtcc aatttgtttt tttgaaaaac tttcggaaat
     9361 caaggacata cacgccccgt attctcctcc cgctcctaca cctgcgataa agcgaaagag
     9421 ataaaccaag tagatgtttg aagcaaaata catcaataaa gaagcaatcg aaaacagaag
     9481 gaccgtttga gaaaatactt tcacacgtcc atatttatca gctatgatgc caaaaaaaat
     9541 acctccaatg agcatcccca aattagtgat cgttgaaatg aaacccgctt gcgtaccact
     9601 aatatgaaaa gaagtaatca ttgaagataa tgaaaaagct aaaaacataa tatccatatt
     9661 ctctaaagca attcccgccg atgtcgataa taataacttt cgctgatacg ttgtcatttt
     9721 tatacccctt ttcctaaaat ttccctaaat tgttaaaagt atatccaaaa aagaataaaa
     9781 aaacaagact tttttatttg aaataataca caaacaaccg atatagataa tccagcttag
     9841 ttttaataga aatgagtatt gacttggagt acacttaaag ttgtagaata aataaaatac
     9901 ataagtaaag tggcaagaaa ttaagagaaa gagtgatcag atgaatatca aacaagtcag
     9961 tgaagaaaag ggcatttcgg ccgatacatt gcgctattat gaacgaatcg ggttgattcc
    10021 accagttaat cggacaaaag gcggaatccg tgattataca gaagaagacc taaaatgggt
    10081 cgactttacg atctgtatgc gaaatgccgg attatcgatc gagtctttga ttgaatatat
    10141 tcgcctatct agcgaaggag aagcaactgt atttgagcgg cgtaagcttt tgattgaaga
    10201 aagtgaactt ttgacgaaaa aaattgctga aatgcaagaa tgtttggaac gtttacaagg
    10261 aaaaatcaag aaatatgatc gtgtgttatc tagaaataaa atggaatgta tcaagtaaaa
    10321 aaatcaagga gcatcgctat ggagttacgc ttactaaaat atttttggac aattgcagaa
    10381 gaaggaacaa tctctcaagc cgcagaagtt ctacatctta cacaaccaac cttaagccga
    10441 caactgaagg agttagaaga ggaactagga acagaactgt ttgtacgcga aaaccgcaaa
    10501 atgatcttga cagaagcagg ttattttcta aaaaagccga gcagaagaaa ttttagattt
    10561 aacgaatcaa accactcaag aattcgagaa cagaaaaaaa caattattca gcggccgatt
    10621 tacgatcggc tgtgtggaag ctgataattc gaatacattg gcactattac tagaaaagtt
    10681 gatccgtgat tatcccagcg ttcaatttca tatccacagc ggaacaagtg atgatatcac
    10741 ggagtgacta gaaaaaagcc tgttagattt agcgattttg ctagatccag tagatacaga
    10801 gaaatacgaa acgattttgt taccaagaaa ggaaaaatgg ggattgctca ttttagaaaa
    10861 ttctgtttta gcaaaaaaag acgtgataac aaaagaagac tttgcgaatg tacctctttt
    10921 gatctcaaag cgtccagaaa tacagcgaat gtttgctgac tggttaggca taaaaaagga
    10981 gcagttgctg atcacaggaa cgtacaattt gattttcaat gtatttgcgc taattgagaa
    11041 tcaggtaggt gctgctttag tcatagaagg tgctgtaagt catctttcaa agcgagtgac
    11101 attcgttccg cttgacccaa tgattgaaac gaactgcatg ctagtctgga aaaagaatcg
    11161 gattttatca cctgtagtca atgaattgat tcaccgtttc aaagaagctt ttgaacagta
    11221 ataagtatct ctaatcgaag aaatttcaat acaaaagcga gacgtatgac agtcttgctt
    11281 ttgtattttt tcatttttaa atcaaaaaaa ataaagagat ttacataact tgttaaataa
    11341 gcgcttacaa acataagaag aacggaaatg aatcagctat aataaccgtt gttctggatt
    11401 gtaatcatta caaagaaatg aagattataa ctttggaggt gttcgaaatg aaagaaaaaa
    11461 agcaaaaatt cgttcaaaaa acatgccctt tattcaatgg ttgggtatga ttctgctgat
    11521 tgtattagct ttattgacga tcattccttt tttatggatg gtttctgctt cattcaaagc
    11581 aaataatgaa gtgttcacga ttccgattca atggatccct aaaacttggc atttagagaa
    11641 ttatctggtt atttggtctc gtattccttt agtgacattt tttaagaata cagctttttt
    11701 aagcgttgtg atcacgttga ttcagttgtt tacttctagt tttgctgctt acgggttttc
    11761 taaactaaat tttaaagggc gcgatatttt atttttagca tatatcggca cgattgctgt
    11821 cccttggcag tcttatatga tcccgcagtt cattatgatg agaaaagtgg gtttaacata
    11881 tacgttgtgg tcgttggtgc tattacaagc gttcaatgct tttggtgtgt tcttgttgaa
    11941 gcaatactac agtagtatcc cagattcttt atgtgagagt gcgcggatcg atggcttatc
    12001 agagtgggga atctatcgca agatcatttt acctcttaca aaaccggcga ttgccagttt
    12061 gacgatcatc acattcgtta atacatggaa cgattatatg gggccattca tttatttgtc
    12121 ttctacggaa aataaaacaa tccagttagg gttgaaaatg tttattgggc tatttgatgc
    12181 agagtacgca ttaatcatgg ctgcctcagt tgtttctgtt cttcctgttg cttttatatt
    12241 tttagcgatg caaaaatatt ttgtagaagg gatcgcttca tcaggaatga aggaatagga
    12301 ggtgattatg aaagagcagc gttttgacaa aaatgtttat atggaattct tttattggtt
    12361 gtatcaatca ttggcaagta gcctttgttt ttggtgtgtc aatatcttgt atatagcagc
    12421 tgcgttcctt ttgtcgatag atggaaggaa tgtcttttcc tttatttttt ctttactttt
    12481 tattggtcca ggttgcatcg tgatgatagg tatgacagaa gaattagcaa aagaaaaaag
    12541 tttttcacca gtacgtttgt tcataaagac gtttcgccaa ttttggctaa agggattttt
    12601 ttattggcta tttgcttgga tagttagtgt gataatgatt tttgattgtt tcttttttat
    12661 acgtttttct tatggaaaat ggttgattcc gttgtttgtt ttacttgctt gtttatcagt
    12721 ttcctttagc atcaactact ggtattttca ggtgagaaat ccagcgtcga aaccaaatca
    12781 agttctacgg atagcgttct actatacgtt gaaaaaatgg tatgtgagtt tactagattt
    12841 tttactattg acaagtttgt ttctcttctt tttcgtaaag ccccaatggt gtattctact
    12901 aggtccttct atcgtatttg gtttgattta cttcaacaat cgtaaattga tgagaactat
    12961 ggatctgtag actttataaa ttgaaacttt caattaagaa tgacagtttt tatgtaaaaa
    13021 ctgtcattct ttttcatttt ttgaaacata tggctaaaca aatgaaataa aacgtgctat
    13081 acttttgaat gaaacaggtt tagaaactgt atcaattatt tgtgattatt tctaactaat
    13141 aattaaaaat aaagcataaa aaggacgatg ttcaatggac gaaaaaatga aacaaggttc
    13201 gaaatctgta gtagaaagtt tgattaatca tcatgttgat tatgtattcg gtataccagg
    13261 tgcaaaaatc gatggtgtat ttaatgaatt agaggatcaa ggtcctgaat taatcgtgac
    13321 tcgtcacgaa caaaatgctg catttatggc acaagcaatc ggacgtatta caggagagcc
    13381 aggagtagtt atcgcaacaa gtggtcctgg tgctagtaac ttagctacag gtttggtaac
    13441 agcgacagca gaaggtgacc cagttttagc aattgccgga caagtaaaac gaagcgatct
    13501 attgaagttg acccatcaaa gcatggataa tgcagcactg ttccagccaa tcacaaaata
    13561 cagtgcagaa atccaagatc cagaaacaat ttcagaagtg attgccaatg cttatcgtat
    13621 ggctaagagt tcaaaaaaag gcgcgagctt tatcagtatc ccgcaagatg tagtggatgc
    13681 acctgtccaa gggaatgtca tcaagccatt gagcgatcct aaattaggtt ctgcttctgc
    13741 agatgatatc cgttacctag ctgaacgcat tagagaagca aaactaccgg tactattagt
    13801 aggtatgcga ggatcaagtg aaaaagaaac gttagccatc aggcaattag ttggaaagac
    13861 agcattgcct gttgtagaga cattccaagc agctggagtg atctcacgtg aattagaagc
    13921 gcatttcttt ggtcgtgtgg gtttatttag aaaccaacca ggagatatgc ttttgaaacg
    13981 aagtgatcta gttattgcta ttggctatga tccaatcgaa tatgaagcaa gaaactggaa
    14041 tgcggaaaaa gatgcgcgga tcattgtgat cgatgaagcg ccagctgaaa tcgatccttt
    14101 tatgcagcct gaaagagaat tgatcggtga tatctcagcc actcttgatt tattgacagg
    14161 ttcattagag cctcaacaag tgtcagaaga tgcaaaagaa tatttagcat ctcttcaagc
    14221 agaattaacg gagcgggaca ttgttcaatc aaaaggagaa gcaggtatct tgcatccttt
    14281 agaagttatt aacacgcttc agtcaaaagt aacagatgat atgactgtta ccgtagatgt
    14341 aggtagtcat tatatttgga tggcacgcca ctttagaagt tatgaacctc gtcatttgtt
    14401 attcagtaat ggtatgcaaa cgcttggtgt tgcattgcca tgggcgattt ctgctgcatt
    14461 ggtgcgtcca aatacacaaa tcgtatcagt ttccggcgat gggggatttt tattctctgc
    14521 ccaggattta gaaacggcgg tacgtaaaaa attgaatatc gttcatttga tttggaatga
    14581 tggacactac aatatggtag agttccaaga aaaaatgaaa tatcagcggg cgtcaggtgt
    14641 agactttggt ccagttgatt ttgtgaaata tgcagaagct tttggcgcta aaggaatccg
    14701 agctacaagt gtagaggaat tagaaaaagc tttagaagaa ggatttgcga cagaaggacc
    14761 cgtaattatt gatattccga ttgattaccg tgataatgag aaattgggag aaacaatctt
    14821 accagatcaa ttctattaag gaagtgtcga catgactgaa aaaatcttat accagcacgg
    14881 gacattagga gctttgatgg ctggcttgat ggatggaaca gaaacgattg ctcatatatt
    14941 agaaaaagga actctgggat tgggaacatt gcatgggctt gatggtgaag tcatcttttt
    15001 agatggcgta gcttatcaag gaagatcaga tggctcagtt gcccaattag atggaagtga
    15061 acttaccccc tatgcagcaa ttacagattt tacgccagat actagttttt ctgtctcaaa
    15121 aactgcagat ggagaacaat tgaaaaaaga catattagta agagaagccg gggaaaacct
    15181 atttttggca gtgaagataa ctggattatt caaaaatatg cacatccgta tcatgccgaa
    15241 gcaagaaaaa ccttacagaa gactagcaaa aatttcagag tctcaaccag aattccaaca
    15301 aagcaatgtc caaggaacat tagtcgggtt ctttacgcct gaattattcc aaggagttgc
    15361 tgcagcggga tttcacttgc attttatcga tgatacacat acatttggcg gacatgtaat
    15421 ggattttgaa gtggctgaag gaaaagttga gattagccgc atctcgtccc ttgtacagca
    15481 ttttccagtt gatgatccga cttttgttca cgaaacgatt gattatgctg atttagcagc
    15541 agaaatccaa caagcagaat agaaaaagac agcgagatgc gttcaaggtg attaacctga
    15601 cgtatctcgt ttttttgagg aggaaaccta gcgtttacaa aaaaactgag ctataatctt
    15661 ctccatagat caaaaaggag cggtttttat catgtggaca gaaaaacaga aaaaagatgc
    15721 atacgaaatt ttattattac agcaacaagg attattagaa gcagaagata actggttagc
    15781 aaatttagca aattcatctg cattgttgaa tgagactttg cctgagactg tctttgcagg
    15841 atattattta ttcgatggta atgaattgat tttaggaccg ttccaagggc gagtttcttg
    15901 cacacgtatc aagatgggaa aaggagtttg tggagaatca gctgaaaaac aagaaacatt
    15961 gatcgttgat gatgtcaaaa cccatgcaaa ttacatttcc tgcgattcag ccgcacaatc
    16021 agaaatcgta gtgccaatgg taaaagaagg taatttaatc ggtgttttag atatcgattg
    16081 cggagtaacg aaagggtatg atgaaatcga tcaagcattt ctagaaaaat atgtagatct
    16141 tttattagca aattttaata taaatgcttt ttcatttatt aataactaaa atcgtctatt
    16201 gaaacaaaaa agaagagaaa tcaagaataa aaagagccgg aaagacagtg ggcatttctg
    16261 tttttcggtt tttttatttt tttataaaat cacaagtgtt attgattttc ataatcatat
    16321 tttaatggta ttatcatttt aaataatgga aaaacattaa ggatggatga aatatgaata
    16381 acgaaactat cattttagga aaaaaatata tttgccaacc tattggcttg aaacaaaaag
    16441 tggttggaga agtaatcagt aaattagaaa attgtgttgt gatctgcgta gacgaatatc
    16501 atgacgtcga tcgcaatgaa atattagaaa aatgcggaaa agtagtggca aaatatactc
    16561 acgtttatga gttggaaaca acagaatatc tatataacta tagcaacaaa tatgaacata
    16621 ctttagtttc ataaagattt aaataaagtg ccttcaaaag cgagaagacc aagcttttga
    16681 aggcacttta tttagttggc agtgattgtt catgattttt gatttattca aaaggttcgc
    16741 ctagagaatc tgtctctgta acgaggcctg gtggagaaac tcgataaaaa cctatactgc
    16801 cagaactgtc ctattttcgg atagacaaac tagtagtttt aaaaagatca tccgatccaa
    16861 tcatttcaaa aaatgcaaaa ctgatatcag gatcatttag ctcaggcttg ttatcgtaaa
    16921 gcatttgtaa aggttgcatt tagttaggag taaacgcgaa actatcatca ttatttgctg
    16981 aagaagatga agaagctaat tcagagtttg tttgaaataa aggagacgtg ctgacagttt
    17041 ctttaccctg ttttttgcag aggtagaaac ttttatatcg gaagtttcta ccggagaatt
    17101 tttctcattt gtagaaattg ttgaatcgat actattcgaa atttgcgtat ctgaaggtat
    17161 gttctttgat gcagaagtcc cacaagcagt aagtaacaat aaatttgata aactgataaa
    17221 cagaattctt ttcattatgt acacactcct tatagtatcg tttagcatac acctatgctc
    17281 agtattaggc aaaatcaatg caaaactatc aagtctttta tagtatctat tagtcttagt
    17341 aatacatata tggagagggt gatatcccag ttcaaaccag atacattggt taccatctgt
    17401 attaacgatt atgcctctaa acgctttttt tatgaaaaaa tttataagtg aaaacgtagg
    17461 tatgatatta tctagaggta atttttttgg gtatttaaat agttgctagg aggata
//
LOCUS       DAFBQH010000056        17428 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.058, whole genome shotgun sequence.
ACCESSION   DAFBQH010000056 DAFBQH010000000
VERSION     DAFBQH010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.058"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..346)
                     /locus_tag="LK694_001946"
     CDS             complement(<1..346)
                     /locus_tag="LK694_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBK5052792.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGY"
     gene            complement(363..665)
                     /locus_tag="LK694_001947"
     CDS             complement(363..665)
                     /locus_tag="LK694_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052793.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(682..1152)
                     /locus_tag="LK694_001948"
     CDS             complement(682..1152)
                     /locus_tag="LK694_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBK5052794.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(1127..1405)
                     /locus_tag="LK694_001949"
     CDS             complement(1127..1405)
                     /locus_tag="LK694_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBK5052795.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(1402..2451)
                     /locus_tag="LK694_001950"
     CDS             complement(1402..2451)
                     /locus_tag="LK694_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBK5052796.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(2408..3337)
                     /gene="tadA"
                     /locus_tag="LK694_001951"
     CDS             complement(2408..3337)
                     /gene="tadA"
                     /locus_tag="LK694_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBK5052797.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            3469..5223
                     /gene="ade"
                     /locus_tag="LK694_001952"
     CDS             3469..5223
                     /gene="ade"
                     /locus_tag="LK694_001952"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBK5052798.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(5321..5983)
                     /gene="trmB"
                     /locus_tag="LK694_001953"
     CDS             complement(5321..5983)
                     /gene="trmB"
                     /locus_tag="LK694_001953"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBK5052799.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(6053..6835)
                     /locus_tag="LK694_001954"
     CDS             complement(6053..6835)
                     /locus_tag="LK694_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBK5052800.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(7039..8250)
                     /locus_tag="LK694_001955"
     CDS             complement(7039..8250)
                     /locus_tag="LK694_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5052801.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(8243..8980)
                     /locus_tag="LK694_001956"
     CDS             complement(8243..8980)
                     /locus_tag="LK694_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5052802.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9118..9552
                     /locus_tag="LK694_001957"
     CDS             9118..9552
                     /locus_tag="LK694_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBK5052803.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            9545..9889
                     /locus_tag="LK694_001958"
     CDS             9545..9889
                     /locus_tag="LK694_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBK5052804.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            10069..11079
                     /locus_tag="LK694_001959"
     CDS             10069..11079
                     /locus_tag="LK694_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK5052805.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(11148..12092)
                     /locus_tag="LK694_001960"
     CDS             complement(11148..12092)
                     /locus_tag="LK694_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK5052806.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(12076..14796)
                     /locus_tag="LK694_001961"
     CDS             complement(12076..14796)
                     /locus_tag="LK694_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK5052807.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(14793..16013)
                     /locus_tag="LK694_001962"
     CDS             complement(14793..16013)
                     /locus_tag="LK694_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBK5052808.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(16294..17139)
                     /locus_tag="LK694_001963"
     CDS             complement(16294..17139)
                     /locus_tag="LK694_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK5052809.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT         5371 a         3469 c         2880 g         5708 t
ORIGIN      
        1 catatccatc ttctccattt tcaaagtgta cataaaaaga aattgtgtgt tctttttcca
       61 ataattgaag ctcagtgata cttgttaaca taggttgatg tccgccggaa gttcttcttt
      121 tacgtatcat tgcctgatat ctttcgatga tatactgttt atcctcgatc gtgtagtgca
      181 gttgctgatt tttcacagag acattgtgac tgctcttgag ttcttcttct aattgtgcta
      241 aaaatattag ccactctttt tgatctttac gctgcaaaaa ttcacttact tggatggttt
      301 gctgaatcaa aagattgaat gtgagcaata gactgctcaa tatcatcagt ccaattagac
      361 attcaatcag cgtaaatgct ggaattactt gttttttcaa tactgatcgc ttctttctcc
      421 atattgacaa acactttttc gattactcca ttattatcga caatttggat atcgtatggg
      481 atcatttctt cccttcgctc tggtcttttg tgatacatcc gctgcgtctt gatatcttca
      541 taaatcattc gaataaatag cagttcctcc ccacttttct tttctgcctg aagcaaaaac
      601 agctggccaa agctcactaa gtagacacag acagaagcga tcataagcgc tgtaagggac
      661 tccaacaaaa caaatcctgt tttattcaat tctttttgta tagcgaccac tccctatctg
      721 gaattgatac gtaatcgtcc gctctttttc ttcccaataa aattgataag cttttagtga
      781 actttcattc ccggttctag cagcaaaagt aatagaagca tggcgtttca aggtaatctc
      841 atcaggaata tctaatactt cccaatttat tttatctgga ttcggaacat gaaaaatgat
      901 ttgattctct tcactattcc aataaaagcc tgtttggatc tgattcacaa tagcgatttt
      961 ttgcgtagaa tagattcgtt tttcaaattg gccgaaaaat tgatagactg ccaattcatt
     1021 cttccatgca gaaaatgcga gaactggaaa agaaaggatc gttgtaagta ataaaatcag
     1081 taagagtgat tcaaacaacg tatatcctga tgctaaaacc ttaccttcaa ggccgctgat
     1141 agatttctgc atgttctcgt ttgatatagc cttcattcaa caattcctcc acagtaggaa
     1201 ctcttccatt attttgaagc gcatagagct ctttttgtgt gtctaccaac tggatgatcg
     1261 cttcacgact ttcttggtct acatgattcc tataacgcga caaattaggg acgaacaaca
     1321 acactaatac actgatgatc agtaagacga ttagcatttc gatcaatgta aaaccgctat
     1381 aataagatat ttttctcttt ttcatggcaa aacctcctct atgcttccgt aaacaggtaa
     1441 taaaacagcg gcataaacta ataaaatcaa agccgctacg cccaaaaaaa tgattggctg
     1501 aataaaatgc atccagtgat gaagacgttt gaaaagctgt tttcttgtcc attcgctgta
     1561 aaaaatcagt tctttaccta aacttccctt tgcctctccc tgtaaaacaa ttttactgaa
     1621 ttcttctgat agaaaacgat aattctggaa ctgctcagcc aatgacatac ctgcctccaa
     1681 tccttgaatc aacttagagg caagatgctg aatcagactt tcttgtttcg tttcctttaa
     1741 gcagactatg atttgcctta attcgaaacc ttcatgaaaa agctttccaa tctctagtga
     1801 caaataagcc gattgataca aggaatatat cggtccaatc aataattgtc tgcttatcca
     1861 ttcactctga tcaacaggag acaacttcat cagacgatac cttactagta aagccaatag
     1921 agtaaccgct aggatgaata gtagaacata ccaatgaaag acttgtatca gctgaatacc
     1981 ccaatgctct tgcatgacca ttcctgaacc aataagctga ggcaaaacga acaaccgcat
     2041 actaaacaag atacctagca aaaaggtgag caatagcatc ggatatgcaa gcagcttttt
     2101 caaatcattg cgaaatcctt caacaagccg aaactgtttg gcaatttcca acaaagttgt
     2161 cggcagattc ccatgacgtt cagctaactc gatctgtacc agttgctctg ctccatatcc
     2221 tatttgcgaa agtacctcac taaacgcttg cccttccttt agctgatgtt ggatatttgc
     2281 caaaataaca ggtggaaacg aacggatcgt gagtaataaa taaagggatt cttgcagact
     2341 aaaaccagat aaaagcagtt ctcccatggt ttgtgcaaac tggttttgct ggcttcttgt
     2401 taatccttta gaatagttct gactgccaaa tattttcctg agcttcttca taactttcct
     2461 cccatgattt ttttactgat tcttcctcca taaattgata actccataaa acagaaggat
     2521 ggtgattggc atcttccaaa agttcttgat aaatgacacc agctaaacat tcagatagct
     2581 cttctttttt ctttgtcagt tcatggattc tgaccaatgt accttcaaga ttgcgtgcat
     2641 gtacggttgc aaaaacccgg tgccctgtta gtgctgctct taccgcagca tgtgcagttg
     2701 attcatctct tatctcgcca atgattagca agtctggacg atgccgtaat gcgagtttca
     2761 aaagtgtatc ataagtctgt gctattttcg gattgatctg tagctgcaga aaaaacggtt
     2821 cttctatctc tacaggatct tcgatagtaa tcacttgcaa tttttcttcc aaagccaacc
     2881 gatacattag agaagtcttg cctgatccga ctggtccgct gaaaaggtat aatccacgtc
     2941 tgacggtttt ctgtaaaata gttgcaaaat ctcgagaact aaagcaacga tggggtgttt
     3001 caaaagtacc gtataatagc cgaatcacta aactctctcg ttgaaggtaa tctcctacag
     3061 aagacaaccg cagacgaact tcctgttttt ctaaagaata agtaaatgca cctaactgag
     3121 cttttctttt ttctccgata tccatctgtc ccaaatactt aaatcttgag atcagttgct
     3181 gtccatctgc cgaagagact ttcttccaga atgttcgttt ttttccatag cgaaaataga
     3241 tgtaaaatac ttctgaagct ggcaatatat atacgtcttg ggcatgtttg gcaaaacttt
     3301 cacttatcat ttgatccgat aatcgtttta tttccattat tctcccctcc acaaaaaaca
     3361 tacgtctttt tgatcttttt gtttcaaata aaatgaattt acaaaaaaat attggcaaaa
     3421 gccgaattta tgttatactt ataaaaattt atcttgggag gcactccatt ggaaacaaca
     3481 ttaaggaaat atatcgaagt agcaagtaaa aaaaagcctg ctgacctagt gatcaagcat
     3541 gctaagatcg tcaacgtctt tacgaaagaa atcatggaaa aagatgtagc aatctgcgaa
     3601 ggaatgatcg taggaatcgg tgaatacgaa ggaaaaagcg tacataatgc taatgggcaa
     3661 tatctagtgc caggattcat cgatggccat gtgcatatcg aaagctcact tttatcacca
     3721 agcgaatttt caaaagtatc cttgcttcat ggagttacta ctgtagtgac agatccccat
     3781 gaaataggaa acattgctgg gagtacaggt cttgagttta tgatagaaga tgcaagacaa
     3841 acactcatga atatcttttt tatgctacct tcttgtgtac ctgtaacccc ttttgagacc
     3901 aatggtgcaa atttaaatgc aaaaaaatta gccccttttt tacaaaaacc cgaagtttta
     3961 ggcttagctg aagtcatgaa ctatcaagca gtagcaaata atgaaacaga catcctagaa
     4021 aagatccaat tgatgcacca gcataagaaa aaaattgacg ggcatgcagc tggtatcggc
     4081 atggaagagt taaatgttta tcctgcagca ggtattcgga cggatcatga agctacaact
     4141 gcaaaagaag ctaaagaacg tttggatctt ggtatgtatt taatggttcg agaagggacc
     4201 gttgcaaaag atctggcatc gctactaccg gcgatcacac ctgaaaattc aagaagatgc
     4261 ttctttgtca ccgatgataa actgatcaat gatttagtaa aagaaggatc aatcgatcat
     4321 atcgtccgtc aagctattgc acttggaatc gatccattac aagccattca gatggcaaca
     4381 ttgaatgcag cagaatgttt tgatttgaaa acactaggtg cgattgcccc tgggtatcaa
     4441 gctgatttct tcttaacaga cgatcttacc aagctgccaa ttcatgaagt tttctataag
     4501 ggaacattag ttgttcaatc tgggcaactc caagaccatt tatttgctga tcattcaaat
     4561 tcatatacgt gttgtctgcc aaaactaaac gctcaaccat taaatccaca ttcgttagag
     4621 ctgcttcttt cttcttcaca agcccatgtg atcgaaatta tcccgaatag tctagtgact
     4681 aaagatttgg tagaaaaagt ggaccgacaa ggcgaacgat tcattccttc aatcgaaaaa
     4741 gaccaattaa agattgcggt gatcgagcgt caccatcaaa cgggaaacat tggattaggc
     4801 atagtgaaag gatttcagct gaaacaagga gcgatagcga caacgattgc ccatgactcg
     4861 cataatctag ttgtcgttgg gacaaacgac gaggatatgc tgtatgctgc taatcttttg
     4921 attcaaaaag gtggcgggat gaccgttgtc aacaaacagc aggaaatagc ttgtgtttct
     4981 cttcctatag gcggaatcat gacacaggaa cctttttcga tcgtctatga acagttgaat
     5041 gaattaataa ctaaagcaca tcaactagga gcagctaaaa cctttgatcc ttttttgaca
     5101 ctttctttct taacattatc tgtcataccc gaattgaaga tcaccgacaa aggattattt
     5161 tcattttctc gcttccaatt gattccacct tgtggaaaaa ccgatgaaat aaatacattt
     5221 taaatcaaat aaatcccaga aaatacatta catctgtaat gttcattttc tgagatttat
     5281 ttatggatat taatgttttt atgaaagcag aaagttagtt tcacagactt ttctttttgg
     5341 tcttatcttg aaattgagct tctactcgat aaatacgttg accttttgaa gaaaattttt
     5401 cttcatactc cgtcataata ttgccttcaa attgactttg atgtaaatct aaccatacct
     5461 gtttgaggat catgccatat tgagaaaaac ttgccagaga atactcaaac aatccttgat
     5521 tatctgtttt gaaatggatt tctccatttg gacgaagaat ctgttcatct acagcaagaa
     5581 aagtcttata tgtcaatcgc cgtttttcat gacgtttttt tggccatgga tcggaaaaat
     5641 tcagatagat ttgatctact tcgctgtcag cgaaatactc tgtcaacgct gatccatcca
     5701 catgtagaag tttcaggtta ggaagcggtt gttcgatcaa cttgtctagt gcaatcgaaa
     5761 caacactgac ctgcatttct actccaatat aattgatttc aggatgggct tttgccattc
     5821 cagtgatgaa ctggcctttc cccataccaa tctcaatgtg gattggatga tcgttttcaa
     5881 acaattcatt ccattttccc ttccacaatg ttggatcact aattacaaaa ttcgggtgag
     5941 ccgctaacat ttccgctgct cccggacgat ttcggacacg cattatttat ctccattcct
     6001 ttcctctaat taaaaaggtc tggccaaatc tccgcagagt ttgggccaaa cctcagccac
     6061 tgaaaattcg ttttaattgt aaaatttctt cgttcatccg tcgatgatct tcacgtttgc
     6121 aatatctggt gatttcttgg agcaaattca tggccgcata ccaatgaaat ttttcaagca
     6181 tttcgtcagt cgggcgtatg ccataactga tcagccactg attccagcta gacagtggca
     6241 cataatttcc taaaatcgtt gcaatatcca ccgcagggtc tgcaaacatg attgaatccc
     6301 aatcgactag atataaataa tttttacata ctaaccaatt acgatggttt acatctccgt
     6361 gtactactga taagttttcc ttatctattg acgggatctg ctcacgaaga aaacgatgga
     6421 caatttgcag aaattgattt ttctggagtt cttccggtaa ttctttttca tatttttgca
     6481 gcatcttctc cggcgtcatg acacgaccac cgatacgggt cagcatggtt ttaagagaat
     6541 ttgaatgatg taaatggtac aaaacatcaa tcacgtcgtt tctttgacca atctcattag
     6601 gagtcagcaa gcgaccttcc agccactctt gtgctgtcaa agtatcgcca ttccccgttc
     6661 gttttgtcca aacaagtttt ggtgtaattc cttcccttga aagagcagcc aacatcggag
     6721 tcgtgtttcg tttaataaag actttgtcct catcacgcat gcctatgtat gtttttcctg
     6781 tgtcgccttt gatgggacgt aaccgccact cttggtctaa ctgaaactcc atcatgcatt
     6841 ctcccgtcct cttactcttt gttttgcagt acttattatt ctagtcgcct gaacactaca
     6901 cgtcaagcac atatacgttc tttttcattt aattgccatt caaaaaacca gcttttctca
     6961 aaaaaaatag aacgaacagc attcttactg tcaacgctct atttttcatt ctcattttta
     7021 ccctacacag ctttgttttc agtctgacat cttcttcaaa cgagttggta catatagttt
     7081 aacaaagagc atagtaacaa tcaaataagc cgctagcgga acccatcgct gctgcccatc
     7141 taatacgact gcagcaatca aaccaaaaat aaccgacgcg ataattaata cccatgtgag
     7201 tagatactga atcgcctgct tcttctgatt cttatggatt ggataaagct gaacgagaac
     7261 catatagcgg aactggttcg ctaaaggtaa cagttggaaa ccgatcaaat ataaaaataa
     7321 gaaaccgata atcagactaa aataaaagtc gactactccg gcaatcaata acccacctat
     7381 aattactaat cggaaataaa gaccactaaa atcagaacct cttgccaatc ttcttgcaaa
     7441 caagtaaaga taagtgtttt ttgattctct tttaatatga gtcaatatag gatccaaata
     7501 gcgtcttctt ttgatctttg ccgtaatttc aggaacatct gtgaataaat tgataaattg
     7561 atagatctga tgcaacctat gttcttctac atgcaccatt ttctcccaat ccaatccttt
     7621 ttcttgttgc ttccacaaaa accagtaata tagacttgcc tgtactaatg cacctgctat
     7681 tcctgcccat attagccaat aaaatgatag aaacaaaaag atcagagaag aaaaaaacca
     7741 gcctaatttc caataacgaa taacttgttg atctagtcga aacaattctg ctctcttgat
     7801 ttgcagatga ctgaatttca aactccataa tatgatcaga tagacgaaaa acatcgaaaa
     7861 ggattgctcc gtactaacca ccactaatgg catggccaat ccgcatgcca atatcaaaaa
     7921 gatcaacgga aaaatgcaag aatagcgaaa agctcgttcc aaataggttt gcatttcttt
     7981 ttctttagga agtaaaaaaa caatatctgc tgcctctgca aaagtagcca tcttacccac
     8041 gaataaacag agcatccaaa atacagagat tatgagccct ccaattgcaa acggtggctg
     8101 cagggttttc aaccaattag aataatataa tcccacaccg ccaatcaaga aaagacaaac
     8161 taaaacaaaa tgatcgttca atacataacg catatatttc atcattttct tttgatgtct
     8221 tgctaaacgt ttttgaaaaa aactactcat gtgcctgctc ctttgttaaa gcaatataga
     8281 tttcgtccaa agaagaatcc ggcagctgaa attcttttct caacatctca agagaaccgt
     8341 ctgctcgtat ctctccgtta tgtaagacca caaaacgatc acaatatttt tctgcagtag
     8401 ctaaaatatg cgtagacatc aaaataccgg ctccttcttt tttcatttct accatcaatt
     8461 caagcaatgc attgatagct agtggatcca agcctaaaaa tggttcgtcg atgatatata
     8521 aagaaggctc gatcaaaaag gcgcataaga tcatcacttt ctgtttcatt ccctttgaaa
     8581 aattagctgg gaaccagtca agacgattag ataaccgaaa agtctccaac aacttttctg
     8641 ctcttttcat tgctatatcc ataggtatat cgtaggccat cgcagtgact tcgatatgtt
     8701 ctctcaatgt caattcttca tataaagaag gagtctctgg aatataccca atttgtttgc
     8761 gatattcttc cgtatttttc tttaaggata gatgattgat ttctatcgtt ccttttgaag
     8821 cttccagcag accgataatg tgcttgatgg tcgtactttt ccctgctccg ttcagtccaa
     8881 tcaaccctac tatctctcca ggctgtacat cgaaactgat atcttttaac acagggagat
     8941 gtccgtaccc ccctgtgaca tgatcgataa ttaaactcat tctttttcct ccgccattta
     9001 tttttattta ttataccacg aagaaaatga cggtattttg tttttttcct tacaatagct
     9061 ttaacttatg gtaaagttaa gctgaagtat ttattcttta tagaaaggat gttttatatg
     9121 gaaaactgta ttttttgcaa aatcatcaat caagaaatcc caagctataa aatatatgaa
     9181 gacgacaagg tctatgcttt tttagacatc tctcaagcaa cgaaaggaca cacattagtt
     9241 gtgcctaaac gtcatgtagc agatattttt gaatatgatc ccgaacttgc aggagaggtt
     9301 ttttcgcgtg tgcctaagat tgctcaagca ttggaaaaag cttttccaga aatgaaaggt
     9361 cttaatatct tgaataacaa ccgtgaacta gcctatcaat ctgtttttca ttcacatatc
     9421 catttagtcc ctcgctacag caaagaggat gatttttcta ttcactttgt caatcatcaa
     9481 gatagttatg gatcggaaga actaaaagct attcaagaaa caatcgtaaa gcaggtgtcc
     9541 tgcgatgatt aaaaactttt tcaaaggact gttatttgga gctgctgctg gcactgtcgg
     9601 aggtctgtta ttggcgccca ggagcgggaa tgaaacaaga aagaaactga tcgaagaatt
     9661 ggaagaagca acaactctga caaatgattt aaataacagc ctgaatcatt ttaaaagcgc
     9721 attgatcgaa acaaaagaaa cagctgaatc tgtgattcct gctttccaag aagcaatcaa
     9781 aaaagatatc gaagatttta aattccaagc cgaaccacgt atcacacaaa tcaatgaaca
     9841 agtagaaaaa ctaaaagctg atctgccaga gtatcctgaa caaatataat aatgattatt
     9901 ttctcaacag atttaactat ataagtgaga aaagtttgcg tatttcctag gaatgtgatg
     9961 aaattttgaa ttttgtttga aactaccacg acaacgtttt taaaggaaat gggatatggt
    10021 atagttaata tataaaaata ataactaatt acaggagtgc aaattttaat gaaaaaaaaa
    10081 tcaattattt tggcagctac tagtgcgttg gctgttttaa ctctagcagc ttgttcaggc
    10141 gatactaata aagatatcgc aacgatgaaa ggcggaacga tcactgtatc tgatttctat
    10201 gatgaagcaa aacttgaatc atcgaaccaa tccttagtac aacggatgat catttataaa
    10261 gtattcaata acaaatatgg cgataaagta accgataagc aagtggatgc agaatacgac
    10321 aaacaagcga aatcattagg tgatactttt gaatcacaat tagaagctgc tggatatacg
    10381 aaagatactt ataaagaata tattcgtaac aatttagctt ttgaagctgg cttgaaagct
    10441 cacgtagata ttacagatga cgatttgaaa actgcttgga aatctttcca tccagaagta
    10501 gaagcacaaa tcatcaaatt atcaagtgaa gatgaagcaa aagatgtgaa aaaatcagcg
    10561 gatgatggag atgatttctc taaattagcc aaagacaaat caactgatac agagacaaaa
    10621 gaagatggcg gtaaagttaa attcgactca acgacaacaa ctattcctgc agaagtaaaa
    10681 gaagcagctt tcaaattgaa agacggtgag atctccgatg ttattactac aacaaatcct
    10741 acttcttatg cgacagaata ctacgttgtc aaaatggtga aaaaccaaaa taaaggcaat
    10801 gacatggata aatacaaaga tcaactaaaa gatatcgcta ctgaaactaa actaagtgac
    10861 aatgcattca caactaaagt aatcggtgaa gaattaaaag atgcaaatgt aaaaatcaaa
    10921 gatgatgcct ttgaaaatgt gttgagtgca ttcacaacaa cttcatcaag tacgaaagac
    10981 tcaagtgaaa ctacagcttc aacaaagagt tctgacacaa aatcaactga ttcaacaaaa
    11041 gaatcttcaa ctgaagaaac aactgattca tcaaaataaa agaaaacaaa aaaagcgaag
    11101 acttatgtct cgcttttttt gttttgaatg aaaaaaataa atttttgcta taaatctttt
    11161 ggtacataga aacttcggtt gtccataccg aaaatccgat cggtgtattt tcctggttct
    11221 gtttgacgaa tcgaagtcaa catcatctgc atcgaagcat cgatattgtc gatttgatgt
    11281 aatatttctg cttccattat tcttggacgt acaggtgagc catattctaa taagccatgg
    11341 tgtgctaaaa ccatatgacg taatacaaga acatcttcgt cagcttcgct gattttcaac
    11401 gcaagacagg ctttagttat ttcttcgtca atcaatacca gatgaccgat caaatttcct
    11461 gcaagcgtat attctgtcga cataggaccg gaaagttcaa tcacctttcc taaatcatgc
    11521 aaaatgatac cagcatacaa caatgctcga ttcagctcag gatattcatc ggcgattgat
    11581 ttccctaagt gaagcattgt caaagtatga taagctaatc cactggcaaa tgcatgatgg
    11641 tttctttttg ctgccggaaa ctcaaaaaat tgtttttgat atttattaag tagatagcga
    11701 acaatacgat tccaatgcgc attggtgatc tcaaacaaca attgattgat ttcttcttcc
    11761 atttcttctt tcttcaaagg ggctctttcc atgtataaac taggctctcc tggctcatct
    11821 acccttgtta agcgcatatg aagaatcttt acttgagggt ttccttgata aacctctctt
    11881 ttaccgttta gaaaaacgac tgtgccggct gtaaatttat tgatttcctc ctccgaagca
    11941 tcccagtatt taccatcgat agtacctgaa gtatcttgga aagtaaaagc aataaatttt
    12001 tttccgtttt tagcgattct tacatctgca cttttgatca ggacaaattg ttcaaactgc
    12061 tcatcgatta ctaaatcacg aatttttttc attatttgct ccttctaatt gttttacgat
    12121 ttggttcatc tcatggtaat agcttaccat ttctttatca gaagaaaaac aaattatttg
    12181 atacttttct ccaaatttag ctaaaagttc agctaattga tactttcgct gatgatcata
    12241 atgaagccag ccatcatcaa tgatgatcgg gcatatcgaa cggttagctt ctaatgagag
    12301 gtaagcaaat ctcatcgcca taatcagctg atctttcgta cctgtagata actcatagat
    12361 gggaaattct gtatatccgt ctgttaaagt cagtgtatca tttttcagta atatcttctg
    12421 ataatttcct tttgttaaaa tacctaaata atcacctgct gctcttaaca actggggtaa
    12481 ttgtctttca ctcatctctg ttgaaagatc acctaagaat gtcccggcga tttcaagtgc
    12541 tccccattcc tccagtaatt cctgtaattt tgctttttgc cggctagctt gttgataaag
    12601 ttcgtccaat gtgccgtctc tttgcaactc ttctacttga agttggagtc gttgccgatt
    12661 ttcacttgct tctttttctt cttgctggag ttgggattgc ttttctttca gttggcgcag
    12721 tcttacagat aatttctctt ttgtcagctc ttcaggaaat agtggagtaa gcatttccga
    12781 taattcattg tatcttttca tctgctgttc ggcttgtttg gctttttgta aaaagacagg
    12841 tacttccgaa ggataagata agcctgcttg agtcaattgt tcgctgtatt cttgtatcca
    12901 tactttttgt ttctttttca attgattgat ttcttgcttc aataaagtat tttgctgata
    12961 gcttctggca aatttcatct gttccatctc ttgtataaaa cgggaaagca ataccatttt
    13021 ttctgaaatt gtcttatctt gtatcggtaa ccattcttta acaaactcaa atttttggtc
    13081 gatttgtcgt agtttttctt gtatttcctg ttctctttta tgtttccgct caaatacgcc
    13141 atataatttc tgataatcta tgacttcctg tccatattct ttcatgagtt tgagcgtatc
    13201 catctttcct aagtgccgat gatccgcaaa acgatatact tcttgctgaa gaatctgttt
    13261 ttcttctcca gttctctttt gtttttcttc tgcttcagct aattgagcta aggctagatc
    13321 aagttgtcct agtttttctt gccacattcc ttttatctca gagaaatcat ttttagtttt
    13381 aatttttggt tgccttttgt agaagaaata gccaatcatg cctgcaccaa ttagtaaaag
    13441 gatcactttc aagggcattg gtacaaatat acctgcaatg attattgctc cgccagccat
    13501 cagcatatac ggtttagatg aagagactgg tgctatctct gactgatttt gtaataatgc
    13561 tggataggtc tcttccaagt cattcacttc tgcttcaatc atctcttgct gttctttagc
    13621 tatttggaga cgcacagaaa tctggctttc tagatcagca atccttcttt cttcagccag
    13681 aagatgcttc cattcttctt catataaaaa ttcttccggt aacggatttt tctgccaatg
    13741 ccacctttgt tccaaaatct tcaattctga atttgtttct ctagtctcaa tttgtaatct
    13801 ttgccattct tcgtttaatt gttccgctgt aaattgaagt tgctggagat cttttagttc
    13861 ctgctcctgc tctaaataaa aataatatct ggctgactgc tgatccatcc cgctatgctt
    13921 ttccagtgtt gcttctaatt cttccaaacg ctcatttgtt tgctgatacc gtttggagaa
    13981 agactccaga tctaaaagaa gttgctcatc aggaagacct gtagtgctgt tttttttgag
    14041 agattgccat tcttcatata aagaaaaatt aagttcttgc tgtctggcta atgaaaattg
    14101 tttgttcgcc tcttttaatt gtgccaataa ctgctgctgt tgcgactcgt attcctgttc
    14161 ttgttggatc aacttgccaa aataagcttc ctgttcttgt ttttgatgaa ttttttcttg
    14221 cagctctttc cattcattta atttttgatt gattagtaat ttttggcctt ttttcttgaa
    14281 taattgttgt gctttctgat aatattcttg ccgcttttgg aatacttgag aactgcccgt
    14341 gatgcctaaa gaaatcagcg catcatgtag ctctttttct ctcaaatgat ctagttccat
    14401 caactgctct tgctggaaag taaatacctg ctggaataac tctttcgtca aaggtgcgat
    14461 cagtttttct agtaactcat cacttcccac ttgatccccc agccacacct tcgattttcc
    14521 gcgattttgc tgcttgtagc gctcgacagc aaattcacca tatacaggat gtttaagcca
    14581 tattttgccg ccataggctg agccgtcttt tggtgtataa tccttttttt tacggccttt
    14641 tgaagggaaa ccaaataata ttgcttgaat aaattgataa atcgttgatt ttcctgcttc
    14701 gtttccccca tagatcagct gattgcctga ataaaagtca aaactttttt gacgccattt
    14761 tccaaaagca gcaatctcga tccttaaaat cttcattcct gatcctcctc aaataaaaaa
    14821 tcttgattaa gcagctgctt ggctttttca agtgtttctt cttgatattc cggcagttcg
    14881 ctcaatattc tagccgcttc ttgatgacta tatacttcat ttaaaatttg ttggaaatct
    14941 tgaggtgtct gatagtattg aaataactgt tccatcaatt tagcagaagc agcaagtggg
    15001 gtcttcgtat aaaaagtatt ctctatgaga gaaatacgcc aaataaagaa atcatttatc
    15061 ctatctgaga attcctctaa caaatagtct aatagctctc ctgattgtat ccgttccaaa
    15121 acatctgtat tgagatggtc ataatctttt aattttatct caattaaact aaatccatcc
    15181 tctacttttg aaagctgctg acgaatgtga acaatgacct cttttgtcgt tcttgctttc
    15241 ctcaatgata tttcctttgt cttccaatag acctctgcta ctttaatcgg atgtatttgg
    15301 caacttcctt tacttaattc aactagtaga atagatgtag aagcttgttc ctttttagta
    15361 tgaccttgag gcgcacctgg ataaacaatt gtttgctttt catttaagac cgttggcaca
    15421 tggatatgcc ctaaagccca ataatcgtat cccttttctt gcatctttga gggttggaaa
    15481 ggagcatagt ttcctttttg agagacgcca ggttccccgt ggtacaaccc aatatggtaa
    15541 tctgtgagtt ctctagaagg aaattccatc actttgtctt tttgaatcca cggttgacga
    15601 taactaaatc cactgagaga gacagtttct ccacttttta tcgttatttt ttttgtctct
    15661 accgtttcgg atgtaaataa atggacattt ttaggaaagg agaaccaata ccgctctttt
    15721 tgatagaaat catgatttcc gaatattata aatacaggta tatggttctt ttctaaccgt
    15781 ttcatctgtt ctgaaaaatg tttctgtatt tttaatgaag gacgattttg atgaaaatta
    15841 tctcctgcaa gtaaaacaaa atcaacgtta tttattattg cctgttcaat gatatttgat
    15901 aaaacggtca gattcgtctt caatagtttt tcctgtacag ttttatcaag agttgtcagt
    15961 ccttcaaatg accgatccat atgtaaatct gccgcatgga taaatttcac cattaaaaat
    16021 ttcctccttt ttttctttta tcataccatt ttcaatgttt tttttcatca ataaccgaaa
    16081 atttgttcgt attatgcaaa gaaattacga taaatggccg acttcaaaaa ataagtttat
    16141 tcgaaaaaaa gattgtgaca tgatttttgt cacaatccta aatatctatg ttatattcgc
    16201 ttcgtttgac actgaagaag tactatttgt actggaattt atgctccatt tttccgtatt
    16261 tagaattcta tgttctatac taccttaatt agtttatatg gtaagaattc tttcaaatgc
    16321 ttgagatact ccgtctttat catttgaaac agtgacataa tcagcttttt tcatgatttc
    16381 ttgatgacta tttcccattg caaaactaat atctgcactt tcaaacatag ataagtcatt
    16441 taaactatct ccgatcgcga ttctcaaagg tttctttaaa ttgtagattg attccaaacg
    16501 atcaaacacc acacctttag atacattttg aggtacgatt tcaatattta caggtgccga
    16561 ttcagtaaca gacacattta gcatcttttt caaatggtta aatatattag ctttcaattg
    16621 acgattagga ataaaaataa aaaatttgta aatgctgata gcatcctttt ctataatttc
    16681 ctcaatatta ttataaggga ttgaacaaat agatttttca tactcaagct catagtttag
    16741 ctctttgata gaaaattcac tccctacctc gtgaaaaatt tttttcattt tatttttata
    16801 attctgagat tcataacttc cgagatttgt atatattttg gttggaaatt tcgataggac
    16861 agaatcaatt ttttttacat catttttatc gaaggaagac tcaaatatct ttttttctcc
    16921 atcaaatcca acagaaccat ttaaccctac tgctggtatc cttaaaccag attttttgag
    16981 aatattatta atgtcttcta cttctcttcc agaacaaatg aatggaaaac agtcttccgg
    17041 taaatgtttc attgttcttg catttttttg actaatgctc tggtcagaat tcaaaagagt
    17101 accgtctaaa tctataccaa ttagtttcat ttcatccatg tctcctatac gttgattata
    17161 aattcactgc ttattgtaag tctaaatttc tcacaaaaca tatactaatt atacctaatt
    17221 taagtaagta caaacatcat tttaagaata ggaaaggaca tttatatact attctctgta
    17281 tttttgattt ataaataaat atgaattgaa tccaaatcag ctatattact attttataat
    17341 aaccaactat aaaaaacgaa gttaggtgaa aaactaactt cgatattcat aaaagactct
    17401 attagttcaa cttagcaaaa gatttaaa
//
LOCUS       DAFBQH010000057        17327 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.059, whole genome shotgun sequence.
ACCESSION   DAFBQH010000057 DAFBQH010000000
VERSION     DAFBQH010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.059"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..392
                     /locus_tag="LK694_001964"
     CDS             <1..392
                     /locus_tag="LK694_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052810.1"
                     /translation="LIISEDVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVV
                     SEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEH
                     YAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            406..807
                     /locus_tag="LK694_001965"
     CDS             406..807
                     /locus_tag="LK694_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052811.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            809..1180
                     /locus_tag="LK694_001966"
     CDS             809..1180
                     /locus_tag="LK694_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052812.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1216..1518)
                     /locus_tag="LK694_001967"
     CDS             complement(1216..1518)
                     /locus_tag="LK694_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052813.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1767..1967)
                     /locus_tag="LK694_001968"
     CDS             complement(1767..1967)
                     /locus_tag="LK694_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK5052814.1"
                     /translation="MKINYTVDLVSFSLVINFCLFSLRLFIEGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(2272..3504)
                     /locus_tag="LK694_001969"
     CDS             complement(2272..3504)
                     /locus_tag="LK694_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBK5052815.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSAAGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVIDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            3761..4330
                     /locus_tag="LK694_001970"
     CDS             3761..4330
                     /locus_tag="LK694_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK5052816.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(4508..4948)
                     /locus_tag="LK694_001971"
     CDS             complement(4508..4948)
                     /locus_tag="LK694_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBK5052817.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLSGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            5106..5870
                     /gene="map"
                     /locus_tag="LK694_001972"
     CDS             5106..5870
                     /gene="map"
                     /locus_tag="LK694_001972"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBK5052818.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            5902..6825
                     /locus_tag="LK694_001973"
     CDS             5902..6825
                     /locus_tag="LK694_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBK5052819.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            6901..8040
                     /locus_tag="LK694_001974"
     CDS             6901..8040
                     /locus_tag="LK694_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBK5052820.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            8033..8833
                     /locus_tag="LK694_001975"
     CDS             8033..8833
                     /locus_tag="LK694_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK5052821.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            8833..9660
                     /locus_tag="LK694_001976"
     CDS             8833..9660
                     /locus_tag="LK694_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK5052822.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            9638..10372
                     /locus_tag="LK694_001977"
     CDS             9638..10372
                     /locus_tag="LK694_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK5052823.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            10472..11338
                     /gene="rfbA"
                     /locus_tag="LK694_001978"
     CDS             10472..11338
                     /gene="rfbA"
                     /locus_tag="LK694_001978"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBK5052824.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            11352..11924
                     /gene="rfbC"
                     /locus_tag="LK694_001979"
     CDS             11352..11924
                     /gene="rfbC"
                     /locus_tag="LK694_001979"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBK5052825.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            11946..12974
                     /gene="rfbB"
                     /locus_tag="LK694_001980"
     CDS             11946..12974
                     /gene="rfbB"
                     /locus_tag="LK694_001980"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBK5052826.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            13072..13923
                     /gene="rfbD"
                     /locus_tag="LK694_001981"
     CDS             13072..13923
                     /gene="rfbD"
                     /locus_tag="LK694_001981"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBK5052827.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            13957..15990
                     /locus_tag="LK694_001982"
     CDS             13957..15990
                     /locus_tag="LK694_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052828.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            16034..17314
                     /locus_tag="LK694_001983"
     CDS             16034..17314
                     /locus_tag="LK694_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBK5052829.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYGEQPDSAHSSRIQRYH"
BASE COUNT         5414 a         2935 c         3544 g         5434 t
ORIGIN      
        1 aattaataat ttcagaagat gtagaaagtc agcaatttgg cactaatgtt attaataaag
       61 taattaatcc agctctttct attaatgcgc ctttaattcc tactgcttta tcattttctg
      121 taacagctgt ggtgtctgag atagaggata cagaaaagat aaaaatagta gaaattgaag
      181 ttttaaacaa gaatgaaaaa caaatttttt ctacgggtga agtatcggta agcttgcctc
      241 ctcaagttaa cgatataaac tttaatataa acgccagaaa tgtcttggtt gaagaagcag
      301 gagagcatta tgctgttgtt aaatttaatg gaactgagat tggaaggcaa atctttgata
      361 tcaaggtcaa caaaccagtg gaaaaaaatt aaggagacga tacggatgct tgatatagta
      421 ccatcaaata cacatcggag tggtagctta ataaattttg ctgcgatttt tgcttgcgca
      481 tcggtaattc tagcatctcc agttgttgtg gatgcaagtt ctactccgaa aactaaaaat
      541 gataatcaac ttgtaattac tacaaaaaat gagattaaca caatttcaag taatgatggc
      601 aaaatttttg atgtgataaa tacggtttta aaaagaaaag ctgaaatcaa tataaaatat
      661 gatgaagatt taaacttgta tttctttgtt atcaaaacaa catctgaatt gttttcctca
      721 gattacgatg ttttggatac tctcgataat gtattatctg actataaata tatggggaaa
      781 tctgtagtag caactttggg ggaataaaat gtttgacttt aatgaatatt tatctatttc
      841 caaacaaatt gaatcagatg ataaatataa ttctaaagaa tcttatagaa gaactgctgt
      901 ttcaagagca tactacagtg cttttaaaaa atctgatgag tatttgaaag aaaattatga
      961 tattatttat aatggaagta gcggaaaagg aagccaccaa accgtttgga atttgttttc
     1021 aacagttaag gaattgaata ccttaggaat acataatagt gggtatagaa tgttagagaa
     1081 aagaaaatgt gctgactata ttcctaatga aaccattacg aaaacagata tggcgctgat
     1141 gaatcgagaa gcagaaaaaa taataaacaa actcacctaa tgaatttcaa gcgagtttgt
     1201 ttgttatttt tttatttaat ctttgtatct ttctggatca acgaaagtat actttatata
     1261 gtcataacgc cgatgatcgc tacgtgcgtc cggcacgtca gtcacgatat caaacaaaaa
     1321 gtatacaccc ttcttcattc tagttttcgc agcaggaatt ttgaaatagt tcttattaga
     1381 atagtagaga ttgattaata agctatcttc gattgctaaa aagaaaactt ctgaattcca
     1441 tactttataa aaatctttga taaatctatt cgaagggtca aatttaaacc ataattgtgt
     1501 ttttccttcc atcagcataa aagttcacct caaaaagagt atacgaacga atgttctttt
     1561 tgtgaaggcg gaatttaata tactgaataa tagaaaacaa gtacttgtgc caaattatgt
     1621 gccaaaaaaa ttcgaattca acaaaatgta aacaaataga aaaccttcta tattaagatt
     1681 tcctgcaata acaaacacaa cagaagacgt gtaatagtta gtcaggaacg tacaaataac
     1741 ccctgtatcc tttgcggtac agggggttat tttgtataag tagacattgt agtttctaaa
     1801 ctgctatgtc ctaaacgttt cgatacgcta agtatattta ctccttgata aagtaaaata
     1861 gatgcatgcg tatgtcttag tccctcaata aacagtcgaa gagaaaatag gcaaaaattg
     1921 attactaaag aaaaacttac taggtcgacg gtgtagttga ttttcatact tcaacagatt
     1981 cgaattaaac gatttcgatt ggcttatacc agtcctatcc gacgaaagat atatcgttct
     2041 taactctagc ggtataactt gaagctttac gtaaagccaa tgggaatgaa ctggagatga
     2101 ctttttatta tttttagtaa tagctgatgg gttaatactt acaactttcc ttttcaaagt
     2161 accatataaa aaaacggtgg ggttgtttag cgaaataaag gatctaagcc aatagcttag
     2221 atcctttatt ctatgcttta tattattttt tgcatgtgtg cgaaagtata ctcaagccca
     2281 atcgccattt cggaaaatcg gtactgtaga gccatcttca cggattccgt cgatatccat
     2341 cttgtcagat cctaccatga aatctacgtg ggtttgactt ctattcaatc cagcttctgc
     2401 taattcttca tcggacattt ctgtaccgcc tttcacactg aaggcataag cagaacctag
     2461 tgctaagtga ttcgatgcgt tttcatcaaa taatgtattg aaaaagatga tgccagattg
     2521 agaaataggt gaagggtcag gtacaagtgc gacttctcct aaacgacgcg caccttcatc
     2581 tgtatcaagc agttttgcta agacatcttc tccttgttcg gcagaaaaat caatgacttt
     2641 tccatcttta aaagtaaatt tcatacccga gatgatggtg ccagcataac tcagtggctt
     2701 tgtacttgaa atgtaaccat ctactcgatg actgtcaggt gctgtgaaca cttcttccgt
     2761 cggcatattt gccatgaatt tttctccacg agcattatag cttccagcgc cttcccaaag
     2821 atggttttta ggcaatccga taatgatatc tgtaccggga gcagtatagt gcagggcaga
     2881 aaattgttct cgattcaatt cttctgcttt ttttgctaat ttttcatcat gcttcttcca
     2941 tgctaagaca gggtcttctt catagacacg agtcgttttg aaaatctgat cccataatgc
     3001 ctcaacttgt ttttcttctg gcagttctgg gaagactttt gctgcccatt gtttaccagc
     3061 cgcagccacg acagtccaac tgactttatt tgcttgagtt gccttgcgta aattcattag
     3121 tgctttgcca gccgctgatt gataagacgc tactcgatgg ctatctacac cagcaaatgc
     3181 atcgggatcg gcagaaacaa cactgatcct gcttgctcct ttttcaagcc attcatccgc
     3241 ttgatcgatt ttagattgtg gtacattctc aatacgatcc gttgcagcat gtaaaagaaa
     3301 ttctctttgg atctggtcgt cagtccattg aacgataacc tcagcggctc ctagtttata
     3361 agcttcttgt gtgatcaatc gagcaagggg tgcctgttcc acgctgatct gtaaaacaac
     3421 tgtgtgtcct ttttctgtgg ctacgccagt ttctgcaatt aagcgtgcat atttttttag
     3481 aagttcatta aaatcagata acataataaa aattcctcct caccattttt tctggtaact
     3541 attagcatga taccatttaa ttatgattca aacaatgagg cctttaaata aaaaaaagag
     3601 gttatttttt taaggttaag tttcatgata tatgttacta cttgctattt cagcttgtga
     3661 agaaaatcaa aaactaattt tgcatatcaa aaaaaacatg ttacaatgaa attaatcaat
     3721 ggtggaagcg ttataaaaaa attaaggaga ttaaaaaaat atggcaagaa aaaagacgat
     3781 tactagagaa cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg
     3841 atttactgcg agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga
     3901 gtttaaaaat atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca
     3961 atatgaagtg ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta
     4021 tatccatttt gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg
     4081 tggaggaaaa gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga
     4141 tccagcttat gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat
     4201 catagctact gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca
     4261 gattgaaaag atgatacaag acagtataga tgctatcttg aaattcaaag aaccaataga
     4321 aatcgattga atatactcaa aagttccaca aatcagtaga taacaagaga tgtgtcaaaa
     4381 taaggaaaag ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca
     4441 tgaatgcaat gaataaaaga gccgtacaga ttgaataaat ctggatggct cttttattgc
     4501 aagtatatta aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt
     4561 cttcttcagc attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg
     4621 caaaagcagc atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag
     4681 atccgcagac accgtaaatt tttcccgata gatccaattc ttgcaagtct tcataaaagt
     4741 ctacgatctc atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat
     4801 ctgcctcctc gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat
     4861 ccagattttc aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc
     4921 tagcatatac gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa
     4981 ttatgaatta taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat
     5041 gtcatttggc taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg
     5101 aaaatatgat tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt
     5161 tattagcaga tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg
     5221 atattgaggt atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg
     5281 gctttgaagg gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat
     5341 tccctcgtaa aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt
     5401 tgaaaggcgg catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga
     5461 tcgaccgatt gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag
     5521 tagggaatcg gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc
     5581 tagcgatcgt tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag
     5641 cagttcctca ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca
     5701 cgatcgaacc tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga
     5761 ctgcgtatac aagagacggc ggactttctt gccaatatga acatacattg gctattacta
     5821 aagatggtcc aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt
     5881 caataggtac gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg
     5941 atgcgcttta ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct
     6001 gtcgtcgttg cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat
     6061 attttaccgt ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt
     6121 cctgagaccg tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca
     6181 ggaggcttgt tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat
     6241 gctttgcaaa ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc
     6301 gtccgtttct tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact
     6361 atcgttcttg ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc
     6421 gtgaacttta tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt
     6481 gtcatcatgt tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc
     6541 attccgggca cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc
     6601 tatgctaggt attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta
     6661 ctcatgcttt ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca
     6721 gttgttcaag aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa
     6781 tggttcaata aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta
     6841 ttgtaaattt atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat
     6901 atgcttgttt cttttagtat ggttataaga ttttttctga caattttatt atcactgata
     6961 ttaacacctg tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa
     7021 agaagaataa ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt
     7081 gttatatcga ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc
     7141 ttgattgctt ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca
     7201 cctaaacaaa aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat
     7261 atacgtattg attccttcac gcttcctttt attggatata tccaactagg ctggcttagt
     7321 tttcccgtaa cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt
     7381 ttggatgggc tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga
     7441 tattttttcc tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca
     7501 agtattatcg gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca
     7561 ggagctctat tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg
     7621 acttttgttt catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt
     7681 tttgctatca ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat
     7741 cttcatcacc gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat
     7801 gcgatggctt tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga
     7861 acgatcatat tgattattgc catgttgttt gctgcaattt tattatttga attgattggt
     7921 ctgatcaatg agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag
     7981 taccgtacgc aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa
     8041 caaattaaac cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg
     8101 gacactagat aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt
     8161 tgacaacaat tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta
     8221 catcgatatc tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct
     8281 gatagctagc gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga
     8341 gacagttaca gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc
     8401 taagattctt aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt
     8461 tgattacgtt ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact
     8521 ggctgtgttt gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc
     8581 aggtagtttt atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg
     8641 tttcttcatg tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa
     8701 gttattgtac acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa
     8761 tgcaaaactg tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg
     8821 gcgttttttc tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc
     8881 agtcaatctc cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt
     8941 attatttacg ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc
     9001 gtttatcatc atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct
     9061 caagcagatg aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac
     9121 gattattttg aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg
     9181 atgatttttt ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt
     9241 acagctcaag cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca
     9301 ggtgttgcct ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct
     9361 ttagattttt tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag
     9421 atccttcctg tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt
     9481 aatcgatacc gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg
     9541 catttatcca gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc
     9601 aaaaaccggc atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga
     9661 attcgtgcgt tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt
     9721 agatagtatt ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc
     9781 agtagatgat acatgggata tattaactga atacgcagaa aaagataagc gaatccagct
     9841 tttttcgggg cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa
     9901 aggagatcta atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa
     9961 gatcactgac tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat
    10021 tgtggacagc gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga
    10081 aggatggctt aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc
    10141 ttctttaaaa cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat
    10201 tggtttgctg ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag
    10261 acatgattta aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg
    10321 gcgtgttcat ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat
    10381 gtttaaatcc ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga
    10441 aatttgaaat caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag
    10501 cggaacacgt ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga
    10561 caaaccaatg atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct
    10621 gattatttca acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga
    10681 tttaggactg cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt
    10741 tatcattgga gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat
    10801 ttactatggt ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc
    10861 gacagtattt ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga
    10921 gaatatgcaa gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt
    10981 aactggcctt tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc
    11041 agaacgtgga gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact
    11101 atccgtagaa cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt
    11161 gcttgaagca tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg
    11221 tttggaagaa attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc
    11281 acagccattg aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg
    11341 agtccttaca gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg
    11401 acgtctttgg cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag
    11461 aacatggatt gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg
    11521 tccttcgggg gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag
    11581 tcactggagc agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc
    11641 aatgggacgg atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat
    11701 atgcccatgg attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt
    11761 actataatgc agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt
    11821 ggccaattga tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa
    11881 aagaatttga agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt
    11941 ttcttatgaa aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc
    12001 attacgtggt aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg
    12061 caggaaacaa agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag
    12121 atgttgcaga tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt
    12181 acgcggcaga atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga
    12241 atatcattgg tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc
    12301 acgtatctac tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg
    12361 gggaaggcga aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt
    12421 cttctacaaa agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac
    12481 gtgcaaccat ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta
    12541 ttcctcgcca agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa
    12601 agaatgttcg tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga
    12661 cgaaaggcag aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga
    12721 ccgtgatgga attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa
    12781 acgaccgtgc aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt
    12841 taggatggaa gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt
    12901 acgaagaaca tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca
    12961 aaaatggaca ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca
    13021 ggcgctcgcc tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta
    13081 ttaactggag gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat
    13141 ctgcaatatg tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg
    13201 gcttacatca cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta
    13261 gataaagcag aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga
    13321 aatgtagcac ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat
    13381 gttttcgatg gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta
    13441 aatgaatatg gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac
    13501 tattatatca tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg
    13561 atgcaacgcc tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga
    13621 ccaacttgga cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca
    13681 tttggtattt atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa
    13741 atattgaaag atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa
    13801 gcaacacgtc cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc
    13861 ataccaactt ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag
    13921 taatttcctt ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca
    13981 cgacgaaaag aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt
    14041 ctaaaactcc gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta
    14101 aacggaagtt cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa
    14161 actgatgtga tttttggaca gaatcgggag gtgcggtcag acgaatggct ggtgcagaca
    14221 cctttttacc tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta
    14281 aacggacaaa acatgatcat tgcgtataat tctcctgcaa aggatattac ggttatcgga
    14341 aaaccattca actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg
    14401 gcgatgaaat tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag
    14461 agaaaaaaag ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag
    14521 tggtggttca tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct
    14581 ttttatcatt atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg
    14641 gtggttattt tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg
    14701 gcgtacttgc tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca
    14761 tgggatctgt tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg
    14821 gttcatttct ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca
    14881 ggaaaacggg taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca
    14941 aattggaaaa ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc
    15001 tatcactttt ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat
    15061 agcgaacaaa agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg
    15121 attaccgttg ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca
    15181 ttaagagcca tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct
    15241 tatatttgga gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat
    15301 gcatggtctt atttctatgc gattacgcat tcgaaaatag ataattattt ttcggatttt
    15361 gaaaccatcg cagttgctgt gttgggaaca gtgattttag catttgcatt atttggctgg
    15421 agacgcttgt tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat
    15481 ccagtcgtag aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa
    15541 atcgatcaac aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc
    15601 ccaacattgg gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg
    15661 aaggaaatcg atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga
    15721 gcttttatcg ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc
    15781 acgttgaatt ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca
    15841 ttaactgatt acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa
    15901 gacggttata tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa
    15961 actcaggaaa atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatatt
    16021 agtagaaagg agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag
    16081 tgtcggtttc ttgcttttgt tgctgacgct agtctttttt gtttggacag cagggttagg
    16141 ttttccagca actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc
    16201 tattttgctc attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt
    16261 tgcgcttgcc cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt
    16321 tggagattta ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg
    16381 gacaaaatgg gatcgcggta tttatctgat tgtctttacg gtcttcctcg gaatcacgtt
    16441 atatcgtgta tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa
    16501 ttcgctttct gatatttgga tcaatacgaa tacaatcgga agttctttga tgactcttgc
    16561 tttattgatc agtggatttg ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc
    16621 agtagcagtt gttgcagctt ttctcgggat ttgggtctgc caatctagag gtgcattagt
    16681 cgcgttgatt gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg
    16741 tgcgccattt attgcttaca cggttacgat tcttttagct ttgccgattt cttatctggc
    16801 ggctgtctct gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta
    16861 tcagacgcta ggagaaaaat ctgaacaagt tttactagga atgaaaacat ttatcttcca
    16921 acgaggaaac caatttttag gaaaccacaa tagttataat tctattttga atatctatgg
    16981 attgatagga tttgggattg ctactttgct gcttatcctt tttattggtc gcttaacgtt
    17041 gaaagcagat ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat
    17101 gcagtcattt atggaggata cattgacttc atacccatgg gttccgatta tttatatcct
    17161 gttggctatg gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat
    17221 ggaagaggca tatgacgata tgacttatga tgatacatct tatggggaac aaccagatag
    17281 cgctcattct tccaggatcc aacgctatca ttgataggca caaaata
//
LOCUS       DAFBQH010000058        17316 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.060, whole genome shotgun sequence.
ACCESSION   DAFBQH010000058 DAFBQH010000000
VERSION     DAFBQH010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.060"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..945
                     /locus_tag="LK694_001984"
     CDS             <1..945
                     /locus_tag="LK694_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HBK5052830.1"
                     /translation="QGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAYQLVETQA
                     PTGYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQD
                     ANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVV
                     ELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGL
                     APGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSS
                     GNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1369..1539)
                     /locus_tag="LK694_001985"
     CDS             complement(1369..1539)
                     /locus_tag="LK694_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5052831.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1733..1912)
                     /locus_tag="LK694_001986"
     CDS             complement(1733..1912)
                     /locus_tag="LK694_001986"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052832.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2299..3411
                     /locus_tag="LK694_001987"
     CDS             2299..3411
                     /locus_tag="LK694_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK5052833.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3489..3782)
                     /locus_tag="LK694_001988"
     CDS             complement(3489..3782)
                     /locus_tag="LK694_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBK5052834.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3950..4264
                     /locus_tag="LK694_001989"
     CDS             3950..4264
                     /locus_tag="LK694_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBK5052835.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4285..6264
                     /locus_tag="LK694_001990"
     CDS             4285..6264
                     /locus_tag="LK694_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBK5052836.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6277..6603
                     /locus_tag="LK694_001991"
     CDS             6277..6603
                     /locus_tag="LK694_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBK5052837.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6619..7137
                     /locus_tag="LK694_001992"
     CDS             6619..7137
                     /locus_tag="LK694_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052838.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7137..8489
                     /gene="celB"
                     /locus_tag="LK694_001993"
     CDS             7137..8489
                     /gene="celB"
                     /locus_tag="LK694_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBK5052839.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8573..10078)
                     /locus_tag="LK694_001994"
     CDS             complement(8573..10078)
                     /locus_tag="LK694_001994"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBK5052840.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10170..10760)
                     /locus_tag="LK694_001995"
     CDS             complement(10170..10760)
                     /locus_tag="LK694_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK5052841.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10939..12585
                     /locus_tag="LK694_001996"
     CDS             10939..12585
                     /locus_tag="LK694_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK5052842.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12634..13353
                     /locus_tag="LK694_001997"
     CDS             12634..13353
                     /locus_tag="LK694_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBK5052843.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13832..14779
                     /locus_tag="LK694_001998"
     CDS             13832..14779
                     /locus_tag="LK694_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5052844.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14791..15711
                     /locus_tag="LK694_001999"
     CDS             14791..15711
                     /locus_tag="LK694_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5052845.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15752..17209
                     /locus_tag="LK694_002000"
     CDS             15752..17209
                     /locus_tag="LK694_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK5052846.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
BASE COUNT         5441 a         2977 c         3542 g         5356 t
ORIGIN      
        1 caaggagctg tttttgagtt acagaaccga gaaggagaaa cgctacaaac cggtctaact
       61 acaggagaag atggaaaact ggcaattgac ggtttagcac caggagcata tcagctggta
      121 gaaactcaag cgccaacggg gtatgaatta gatgcgacac cgatcgagtt tgagatcgaa
      181 agaagtcaga cagcggtggt agaattgacg aaagcaaacc gacttacacc aggcggtgtc
      241 gtgttgacaa agatcgatga ccaaagcggg gaagtcctgc aaggagctgt ttttgagtta
      301 caagatgcca atggaaccgt tctgcagtca ggtctaacca caggagcaga tggaaaactg
      361 gcgattgacg gcttagcacc aggagtgtat cagctggtag aaacccaagc gccaacgggg
      421 tatgaattgg atgcgacacc aatcgagttt gagatcgaaa gaagtcagac agcggtggta
      481 gaattgacga aagaaaatcg acttacacca ggcggtgtcg tgttgacaaa gatcgatgac
      541 caaagcggag aagtcctgca aggagctgtt tttgagttac aagatgccaa tggaaccgtt
      601 ctgcagtcag gtctaaccac aggagcagat ggaaaactgg cgatagatgg cttagcacca
      661 ggagcatatc agctggtaga aactcaagcg ccaacggggt atgaattgga tgcgacacca
      721 gtaactttca agatcgaaaa agaacaagaa gtggcgatct ttttaacaaa agagaataga
      781 aaagttgctg atagctcaga tataagagga aacaatccaa caagcagtgg aaataagcat
      841 cttccaaaaa ctggagaaac catcattagc cagtttatat tgtcaatatt gggtgtgcta
      901 cttgttttca tcagtataaa atttagaaag aaacgaaata tatgagctaa aataaaaaat
      961 tgaataagca tagtttttaa aatcaactag tacccatcaa ttgaatggaa actaccgatt
     1021 ttagaagggg tatacaaaca aatattagta tcggagtaga gaaggttgaa cccaaccagt
     1081 aaataagtaa agacaatttt gtgctgcgtt gagaagtgaa aaataaacct ttgtagtaat
     1141 actagttaat aaaaaatata caaaaacact gcgaaaatca ttcttgagtt tcgcagtgtt
     1201 ttttgcctaa tgactcaagt ttattgggta atactttgat tttctttcta caaataatca
     1261 gcgatcagtt gtaatttttt ctctagtttt aatggctctt tgtcaataag gagaaaaaca
     1321 agtcgggtct tttgtctcgg aaagcttcaa ccaactcgtg aataaaggtt acgcctcctt
     1381 gaaaggcgtg gaaaaaccaa gtaatcgatc aatcaccaat ttcgcatttg gtagaacatg
     1441 tttggtgagc tgaaagtagg tggcgttcat gtctgtaact aagaattcta tttctttagg
     1501 attagtgcag ctaaggaaat agtttgttaa tcgaggaatt ttttgtgtag gtaaattttc
     1561 tcgattaatt tttctgtctc gccatccgcg gaaataaaac tcagtttatc ctcaacggaa
     1621 acgtgagaac gaaattcaca accctcaaca ctcaagacag aattcttttg gaagactttg
     1681 gtaaatagct tctcaactct ttcaatgtgc gaataacggt tgtcaaagat acctaacaac
     1741 ttttcgaaat aaaaaataac gacacttttt cagtcagtaa agaagtgatt ttatgtctga
     1801 catgatttga aatcgagtgc cttggacgga taaaataact ttgagcgttc caatggcttc
     1861 gacagttttt acaggtatag cgctgctttt ttaggcgtat aaccattggc atatgattat
     1921 attattcaaa gtggacaatg gtttccttct tcttgttttt caccaccatt aatttcctat
     1981 ttccatctac cacagcagaa ccacaatttc tacagacaca tggagcaggg aagagaactg
     2041 cagcaacgat caatgttttt ttcttctgta aggtttcgta agagacctct gtaatcatca
     2101 aatttttgtc tattgatctc agaatttttt tgatagaacc attcataaag catatcgttc
     2161 tctcagttgt ttattttgtg gtgatttaat catacaagaa aacgatatgt ttttttataa
     2221 aaacatgaat aggaaaaata gttgacaaga aaatgacaac gccatatgat gttccatagt
     2281 atttacaacc tgtcatagat gactgtaaaa aataaaagta aaaagaaagg acgacaacag
     2341 gtggattttt atcagtctct acaactagat ccgtttattt taaaacaaaa aatacgagaa
     2401 gcagcttcta aaaaagaaaa gtgcatgtat atttgttcat tgtttttacg atcattattc
     2461 atcgtattgt ttgccatttg ttttatcatt ataattacaa cattatttga atcgaagcat
     2521 aaaccatacg ctgtggtttt attctgtatg ttgatgagta ttcgttttgt tgactttggt
     2581 tataagattt cccattcgat cataggttta gcaatcgtca tgttctcact attagtagca
     2641 ccgtatgtcc agctgatcaa atggtcagtt atgggcatgt tgattcattt tattttacta
     2701 agcagcatat taatggcaac cgctagtgat cccaaaatgg ggaatgcttc tttgtacggc
     2761 ttttcatatc tctttattgt ttattcgtta cctaaagatt cgttgaacaa gaatttcttt
     2821 acacaaacaa gtagcttatt atttttgttt ttttgttggt tttcagttct cctttacaga
     2881 aaacatcgag aaaagaacaa agacaaaagc ttgtttaaag agattttttt gaaaggcatg
     2941 tattcgcagg aaaaaatttg gatgctcatc tatgcatttg gcattagtct tttgatcgtt
     3001 gcaggagagt atgtcccttt tcagcgattg atgtgggcag ggtttgcatt ttcttctatt
     3061 gtatctagct atggattgat gtcgattggt ttcaaagaac gagcagtaga ccgaatcatt
     3121 ggctcattga ttggatgtgt tttatttatt ggtatcagcc aatttattcc ttttaactgg
     3181 gtcggtatat taggtggtct agctttaggt gtttgttcta cctatagata taaaacagtt
     3241 tttaattctt ttggtgcttt ggcaatcact gccagtttat tcggggttcc aggagcagta
     3301 acgatccgca tttttgagaa tatccttggg gtatgtttag gaatcatgta tattggggtg
     3361 actgaaatac ttatccgaaa aataagagaa aaacacggtt tgaatcatta gatgagatcg
     3421 aataaaaaat aaggaaaaag tgaagataca catggatgat aaattgctat cattccatgt
     3481 gtatcttttt acggtaaatc agtgaaacgt tcgactttca tttttaattt atacttttta
     3541 cggagttcgt ttatttgagc catcatttcg gatttgtgat gcttttcgat tgcttcttca
     3601 tctttccaac ggtcgatcag taaaagagat tctggatctt ctagtggaaa gtaatagtca
     3661 tatttctcgt ttccttcttc tgcacgaatc gctttgacaa tgtttctttc gaccatttct
     3721 tctgcaaatt ttctggcttg tccatttgta cttgtataca aaatattcac tgtgatactc
     3781 actaaatccc ctcctgaaaa aattctatca ctagtatatc acggtgccag aaattattat
     3841 tcaagatatg atacacattt tttccgcaat gctacggaaa tatatatttt gaatagaaaa
     3901 cgcttacaag atatgcttgg tttataaaaa taaaaggagt gattgattta tgaaaaaagc
     3961 attgattatt tgtgcggcag gaatgtcatc ctcgatgatg gcctcaaaga caacggattt
     4021 ctttaagcaa aaaagagaag aggtactcgt agatgcagtt tcagctacag aaggcgataa
     4081 tatgattaag aatagtgatt ttgatttatt tctgatcagc ccgcaaacga cgatgttttt
     4141 ggataaattc aaaaaattag gagaatctgt tggaaagcct gttgttagta tccctttcca
     4201 agcgtatgtc ccaatcccta caggcatcca gaagttagca gaggtgatcg aagagaacat
     4261 atgatggaat aggagtggat gataatgaaa cagaaggaaa aattgctgtt gagatatttg
     4321 attgatcaca agaaggagta tgtcaccagt caacgtttag cttctgaatt gttattatct
     4381 gatcggacaa tccgtaatta cttgcagcgg atcaaagagt tagtagaaaa aaacggaggt
     4441 aagatcattg ctaaaccagg ttatggctac caactgcata ttttacaaag actgacattt
     4501 gacttgtttt tgagtcggca agaaatagta cctggatatg ctagagaacc gcaaggattt
     4561 tatgagtccg aagatcgaca aaagtatata ttgaaccaat tgcttttgga agatgccatt
     4621 ctttttatgg atgatttggc agaagagctt tatatcagtc gttccagttt gacaaaggac
     4681 atgcaagaaa tcaaggagcg attgattcca tattcactaa aaatcgtttc taagcatggt
     4741 caagggacat ggatcgaagg ggaagaaaga aatcgacggc attttatcat ggatactttt
     4801 tttggaaacc attatggaaa ttcattgaaa gaatatttag ggcaaagccg ttttttccca
     4861 gacatcagtt ttgaagagct agtcattatc attctcgatg aaacaagaga agctaagtta
     4921 aaagtttcgg attttatcat acagaatctg actttacact tggctttggg gatcaaacgg
     4981 ctccgagaag ggtttgaaat caaggaatta ggcatcgaac aagaagtttc taagcgagta
     5041 gagtatcaag tggcgcagcg gatcgttcaa cgtattgagg taatcgcagg tgtatcgttt
     5101 cccgaagaag aaattgctta tttgacatta catctcatgg ctaagtcgaa tcacaaagaa
     5161 aatcaaaatg atcaggaact atcaaaggaa ttagcgaaag ttctgaaaag attgtctcag
     5221 gaatttcatt tttcattagt agaggatctc cagcttttca atggactact cgatcatttg
     5281 tcaccgatga tgattcgatt gagccgaggt attcgcattg agaacccatt gactgaggag
     5341 ataaagaaag aaaatcccaa agtctttgac gcagtaaaaa ggcatttcag caacatgccg
     5401 gctttgaaaa actataccat caatgaagat gaatgggcct atttggcttt gcatctcatg
     5461 gcggcattgg aaaaagaaag agctgcgcat aaactacatg cattgattat ctgtgcaacg
     5521 ggttacggca gtgcccaact attaaagaat cgcgtagtca gtgaattcgg taaaaatatt
     5581 acagtggtaa gtgtaaaagg ctattatgag atcaatgaaa caactttaaa aggaatcgac
     5641 ctgattattt cttctattga tctttcgaca atgtttttca aaattccagt tttacatgtc
     5701 agtatttttt tgaatgaaga tgatgttcgt aaaatacgaa aggtagttgg agaatctata
     5761 ccctatcatt cctcagatag accgatacca gcattttctc tacagcaaaa aaagcagatt
     5821 tacaccgaac agttgtctga aaaatttttc aagacttata cgtacgaacc gaaaaaggaa
     5881 gaaattcttc atgatttgct gcaaaagctg agcataaatg aagaagaagg atatataaga
     5941 gaaatgcgaa agcagatcca gcaacgtgaa aatatgggac aaatcatatt cagtgataca
     6001 gtggcagtcc cacatcccgc tgttccagtt ggaatcgaaa caaaggtagc cgttgcattg
     6061 attccttctg ggatgcagtg ggatcaatat caagaaattc attttgtttt tttagtttcg
     6121 ccttcttatg tcgaaaatga aggaattacg gtcgtgacta aagcaatcgt taggttagtt
     6181 gaccagctaa aagtacagca ggaaatctta gctgaaccta tttttgagaa tttcagcagt
     6241 aaatttatca aactgatcga ataaggagga aaacagatgg ctgaaaaaat aaacagtgaa
     6301 acactacaag tgacttcttt tgatatcatt ttccatagcg gaaatgcacg gacactgata
     6361 catgaagcat ttcgctcgat gcgtaaagag atgtttgaag aagtagaaga gaagttgcga
     6421 caagcaaatg aagagattct tcatgcgcat cagtcacaaa ctcaacttct gaaagaatat
     6481 gccagcggga caaaaataga aatggaaatt atcatggtcc atgcacaaga tcatctgatg
     6541 acgacgatga cattattgga agttgcacag gaaatgaatt atttgtatca aaaaaataaa
     6601 tagaaaggaa ggatcattgt gcaggataca gtaaaaagga aaatcgaatt agaaaaaaag
     6661 caacttagtt tgaaaaacat gtatttcaat cgttatctaa tcgtacgtta cctgactgcc
     6721 tttttctttt tcatgaacat gcaatggatg attctgcttc tatcagctaa aagtttagga
     6781 agtagtttac cgatgattct gattttagct attttgcctg cagtaggaga acaagtaaag
     6841 ctttatcgaa aacaccaaac gaacgttcca tggacaaagt ggtatttttt gttccagagt
     6901 gtctttaatt tattagtgat tccagtgttg tttacctcag gatttacatt cttgtatcct
     6961 tttatggcaa ataacaatgg aggacagtta tttgttttca ttcttgttgt aagcggtatt
     7021 tttgccagtg catttataca gtatcgttta aagaaaattt cactcaacca agaccaacag
     7081 tatatccgta ttaaacaata tgaaaaagcg ttatatttag gaaaggagaa taactaatgt
     7141 ttgatttttt gcagaaatac ctgatgggac ctatggggaa aattgcgcaa cttaaaatcg
     7201 ttcgggctgt catggcagcg gggatggctt ctattccttt cacgattgta ggctcgatgt
     7261 ttttggtttt aaatattttg ccattaccgt tcccattttt agaaggattt tttaatgcaa
     7321 cttttttcaa agttagtgac ttgtacatga tcgtcaatac aatgacgatg gggattttat
     7381 ctatttactt tgccattgtg tttgcttatg aattaacgtc aattgaacga gatgaacaaa
     7441 aattaaatgt taatccgctc acgggggcat tactatctgt ttttgcattc tttatgtgta
     7501 tccctgaatt gatcatgact gatggtaaaa tcagcttgat tgcttctatc aatgagacag
     7561 aaactgtagt aaatggtatt aggatgggct cttttgtcga acgtttagga acttcaggaa
     7621 tctttacagc tattattatg tcgattattg cggtcgaatt gtattgtatg tgtgtcaaga
     7681 gaaattgggt cataaaaatg ccagatgttg ttccgccagg agtttctcgt tctttcactg
     7741 ctctgatccc aacatttgtc attgcgtttg tggtgatgat catcaatgga ttattagttg
     7801 cactaggaac ggatatcttc aaaatgattg ctatcccatt tgggtttgtt acgcagttga
     7861 cgaatacatg gcttggtgtc atggtgattt atttcttgat ccatgcattg tggatcgtag
     7921 ggatccatgg agcgaatatc atcacttctt tcttgacccc gatcgtatta gctaatatgg
     7981 cagctaacgc acaaggcgca aattatccgt tggcaggaga attcaacaat tcttatgtaa
     8041 ccgttggtgg atcaggtgcg actttaggat tgatcatctt tattgcattc ctcgcgaaat
     8101 ctgatcagct gaaagtgtta ggaaaagcct cacttgtacc aggattattc aatatcaatg
     8161 aaccaatcat tttcgggatg ccaatcgtct acaatcccta tttagcggtt ccgtttttct
     8221 tagctccgat ggcttctgct acactcgctt attttgccat tcggctggaa atcgtcaaac
     8281 cgatgcttgc acagatgccg tggccttcac cagtcgggat cggagcattt gttggtagtg
     8341 gtggtgattg gaaagctgct gtgttagcag tcatttgtgc aatcatcgca ttcattatct
     8401 ggttcccatt tatcaaattc tatgataata aattattaaa tgaagaaaga gaaaaggcag
     8461 cagaattagc tagtgaaaag ttaaaatgat ttctaagaaa aaaggaactg tgacaaaaaa
     8521 tcacagtccc tttttttctt agaaatggga aaccactagt atataaatac cattattctt
     8581 caatcaaccg cttcacgatt gcataatccc cttcataagg ttcatctaca ccattgattt
     8641 tttgataggg atccacgcct tttcccgcaa taacgactga atcttgcgga gcgctttctg
     8701 ctaaagcgcg tcgaatcgct tcaggacgat ccatttcata atgaacgatc aaatctggat
     8761 tagtaattgc ttcattgatt tcttccgcga tttttttcgg atcttcaaat gctgggtcat
     8821 ctgccgtcaa aaaggcgatg tcagctgttt ctgatagcac ttttccaaaa tcatgtcgac
     8881 gggagattgc tttgtttcct gggctgccta atacaacaat tacacggcca tctggatgtt
     8941 catttttggc aaatttcagc aatgttttca agcttagata attatgtgca tagtccacat
     9001 agacatgcgc tccattggat tggatcaatt gatccattcg gcctggtaca cgagcttctt
     9061 ttaatccgtc ttgtgcatct tgactggagg cacctgttag tgctgcggca atgatcgcac
     9121 tggctgcatt ttcttggttg aatcctccag ccagcaaaat atcataagca gtatcgcaga
     9181 tagctaagcg atcttcatga gaagataaag taaaaccgtg agtaccttta tctgaacgga
     9241 tcacttgata attgctttct tccgaacgac cataagtgat cgttggaatc tggaatagat
     9301 cggctgtttc cctaagtaaa tcaaaatagt cactttcatg atttaaaaca actgtttttg
     9361 aattcaagat caattgtcgc ttgcaataga aatagtcatc aaatgtcgga tgttcgatcg
     9421 gactgatatg gtcaggcgag atattcaaga agattccgac atctaaagtc aagccgtata
     9481 cgcgttgtgt tttgtaagct tgtgaagaaa cttccatcac tagatgagtc ataccatttt
     9541 caactgcttc tgacatcata cgatataaat ctaatgactc aggcgttgtc aaatgggatt
     9601 tgaaatatgt tttgccatct aacgttgtat tcatagtaga aagcaatgct gtttttttat
     9661 ttgttgtgtg atccaagatt gcttttgtaa agtacgcagc agttgtttta ccttttgttc
     9721 cagtgaatcc gatcactttc aatttatttt gcggatagtc gtaaaaagcc atagcaagaa
     9781 gcgccattgc tttacggata tccgttacga tgattcctac tgctgaattt tcgtatggtt
     9841 gttctgatac ataatattga ataccctgtt cgattgctga atccaaatat tcttctttga
     9901 aattcattcc tttacagaaa aacaaagtag aagcatcagc ttttcttgaa tcataactta
     9961 actgaagaaa ttcagctttt tctggtacat caagatgcca gcctcttgtt gaaacaaatt
    10021 cttttaatag attttctttt aatagtaaat gacggatgtc ttctaaagag aagctcattg
    10081 attgatccct cgctttattc aatttcttgg tttattatag cacactcttt ttaatatatc
    10141 gtttgtttcc gctatgacaa aatccagctt tattcaaatt ttcttgacac aggggacaag
    10201 cggatgagta ttttcataaa caatgaactg actgtaatag ataggagaac gaatagtgtc
    10261 tccaacaatc caaaaacata ataattcaaa aagacgataa gactattggt aagtaaaagt
    10321 ccttgcggca ttggaagccg aaaaaatttg tggaaaataa tcggcggtat cgttataccg
    10381 ccatttgagg ccccgactgt gtacaatgtg taaaccccaa gactgaacaa aagacttcct
    10441 gaaatcagtg aaaagaaatg tgaggagatc atttccactt ttggaacaat ctctaaaaca
    10501 acaggaaaaa gcagacttcc cgctacagtc ttaaaaaata ctgattttcc taataggaca
    10561 gcggctagaa gcagcatcat aagattgatg ctccaaacag taactgagcg ttggatagaa
    10621 aaggccgttt caactaaatg tccgatggcg cccactccgg cggaagctat atcgtgaggg
    10681 agcaagaacc aattcaatcc aactgtgata aagaaaagac ctaaaaatat aacgggtagt
    10741 ttttttattt cttgtgtcat tgatatctcc tataaatgtt agccataaaa ggctgtatat
    10801 ttctttactt taccaaaaaa acgaaaccga attcaaaaaa acaattgttc ttataaaagt
    10861 tatataaaat cgattgccag tttctgtttt accaattaaa atgatagtaa tgttttgtag
    10921 aaagttggaa tctgaccgat ggataatcaa agaaaaccag cattagatca attaacaccg
    10981 caagaaaaaa tggcacgagg ctctgcttgg atgaccgcca gtaatatcat ttcaagattg
    11041 ctaggcgctg tctatatcat tccctggtat gcttggatgg gcgaaaatgc taaggctgcc
    11101 aatggattat tcaatatggg atacaatata tatgcattgt ttctactgat ctctacagca
    11161 ggaatacctg ctgcgattgc aaaacaaact gcacgctata attcattgaa tgaatatggc
    11221 acttcacgac gattatttat ccgtgcatta cagatgatgg gcgggttagg cctgttgttt
    11281 gctttgttca tgtacatcgc ctctccttgg ctagctcatg cttctggtgg cggagaagaa
    11341 ctgattccaa cgatgcgttc tttgagtatc gctgtattgg tttttccttg tatgagtgtg
    11401 atacgagggt atttgcaagg caatcaagaa atgatgcctt atgcgctgtc gcagattgta
    11461 gaacaggtgg cacgtatttt ttatatgcta ttggctacat tcatcatcat gaaagtcatg
    11521 gatggtgact atgtgacggc ggtcacgcaa tcaactttcg cggcatttat cggtatgcta
    11581 gcaagtattc ttgtcctttt gtattttttg aagaaacatc aagcgtatac atcggcgttt
    11641 attcactaca gcgaaaacaa aataaatatt gcaacgaagg aattgttgtt agataccgtt
    11701 aaagaagcga ttccgtttat tattgtgggt tcaggggtaa cgatcttcaa attagtcgat
    11761 cagttcactt ttatgaagat catgtctgca tccaccgagt attccaatgc acagttactg
    11821 gatctttttt cgatttttag tgccaatcca gataagttga cgatggtagt tattgcattg
    11881 gcaacatcga tcgcgtctac tgggttaccg ttgattacag aagctgttac gttgaaagat
    11941 cgagtaggtc tggcaaaatt gacaagcgga aatttacagt tgttttcatt ttttatgttt
    12001 ccggcgacat ttggtgttat gttacttgcc tatccgctaa atacattatt ttatacaccg
    12061 gattctctag gaagtaatgt actgatccaa gcaagttttg taggactctt cctaggacta
    12121 tatatgctag tttccaacat gctgcaagga atgtttgaaa acaaggcggc aattaattat
    12181 ttggttattg ggtttctagt gaaactcctt cttcaatacc cagccattcg tttatttgaa
    12241 gtatacggcc ctttattggc gacaatgatc ggatttgctg tgtcttgtac cttgatctta
    12301 aagagaatcc atgaagtagc ccgctttaaa ccaagttttg tctggcgcag gacattgctg
    12361 atctttattc ttaccttgat tatgcttttg gcttcgtgga tcacaaagat gatttttggt
    12421 gcttttttaa acgaagatag caaattccag tctctattgt tgattcttgt agtagcaggt
    12481 ataggggctt tagtgtatgg ttatttagct ttgaaaatcc gcttggcaga aagactacta
    12541 ggaccaggaa tagcgcgttt acgacataaa ttaaaaataa aataagtgaa tcagaagagc
    12601 tagattgatg ctcttttgaa atgggggaaa cgcatgcgtt tagataagtt tttagcagat
    12661 atgggacttg gcagccgcaa agaagtcaaa ggattaatca aaaaaggaac gatcgaagtc
    12721 aatggacaag tggtgaaaag cgataaagcg caagtaaatg aattcgaaga tgcggttact
    12781 tatttaggag aaatcattac gtatcaaaaa gatttttact acattttgca taaacctgca
    12841 ggagtcattt ctgctactca ggataatcat gatcaaaccg taatcgattt attggaagac
    12901 caggattatc gggaagatct ttttccagtc ggccgattag ataaagacac agagggcctc
    12961 ttgattttaa cgaatgacgg ggtgtttgcc catcagttac tatcacctaa aaaacatgtg
    13021 gagaaagaat atctagctga ggttagcggt ataatgacag aagaagatat ccgtttattt
    13081 gcagaaggac tgattattga tggcgatgag cagacattgc cggcaaaact ccaaattgat
    13141 caagtagatc aagagacaga aacttcaatt atccgtctga ttttgcatga aggaaagttt
    13201 caccaagtta agcgaatggt caaagcagta ggaaaagaag tcttatattt aaaaagaatc
    13261 cgtatgggaa aattttggct gcctaaagat ttgaataaag ggcaatatcg acccatgacc
    13321 aaagaagagc tagaacaagt gagagaaaaa taattgaatg gatagctaaa agaataagca
    13381 gagtagttgt agcgccatct gaaaagcaga cttgtccgtc tgactttcgg atggcttttt
    13441 tgtgaaattt cggcttattt ccgtagaagc tacttctgga acaccgtttg ttcggaataa
    13501 gggactgtga caagactttt gttacagtcc ccattgttcc ttgtgactca gagttagata
    13561 gtaaggacac gatctcccta atactcaggc caaaacgctg aattcacaac ctctgataaa
    13621 cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata tgaggaacta
    13681 ttttcccgtt tttttctcta atactcaggc caaaacgctg atttcacaac ctctcctata
    13741 ctttgtttca ttttccctat actttgttta tttattctca ttattgaaag cgctataatt
    13801 acgttgcgaa tgaatcgaaa ggggttttta gatgaaaaaa cgaaaggggt tcaatcattt
    13861 ttttatgaat ctatggcgtt ataaagcact tgtcctgatg gctgtaccgg gtatgctttg
    13921 gatgattttt ttcttttaca tacctgtaat ggcgaatgtc gtagcattca aaaattttca
    13981 tatttcagca gatgggttct tggctagctt gagggaaagt ccttgggtgg gttgggaaaa
    14041 tttcaaattt ttattttctt cggatcaagc atttctcatc actaaaaata caattttata
    14101 taatgtagca tttatccttt tgaatctgct gatctcagtc gtatttgcaa tcattatgag
    14161 cgagttgaga aataaacgac tcgttaaagt ttatcaaacg atgtccttac taccttattt
    14221 tctatcttgg gtcattatca gttactttgt ttacgctttt ttaagtcctg acaaaggaat
    14281 attcaatcag tggatcactg gacatggagg agaagctgtc aattggtata atgaaccgaa
    14341 atattggccg tttatcttgg tgttcattgg tacatggaaa gggattggtt ataacagcat
    14401 tatttacttt gcatctgtta tgggaatcga tccgacttat tatgaagctg ctatggttga
    14461 tggagctagc aaatggcagc agatcaaaca tgtgacgata ccacaactta ttccactaat
    14521 gacgatttta acgattttag ccgtaggaaa cattttccga gctgattttg gcctgtttta
    14581 taacattcct aggaattccg gtgcattata tgatgtgact caagttttgg atacgtatat
    14641 ctacaatggc ctgacatcca caggagattt tggtatgacg gctgctgcag gactttacca
    14701 atcagttgtc ggctttgtct tgttgatgat cacaaatacg atcgcccgac gttttgatag
    14761 cgattctgca ttattctaag gaggaaaaaa atgaaaaaaa agaaagtgga gagagtccaa
    14821 gtccgctctt tcaataagac ggctaatttt ttctctaata taatgattgc actctttgct
    14881 atttcctgta ttttgccatt tatatttgtt atcgccatct cttttaccga tgagtccata
    14941 ttgtcgactc acgggtacag tttttggcca gaaaagttca gtatatttgg ttatacgtac
    15001 ttgttcgacc agatgcagga taaaattttc caagcgcttt tcgtcactgt acttgttact
    15061 gttttaggta cactgatcaa tagcacggct acttctttat atgcttatgc gatttcacgg
    15121 tcaaactttc cattccgtcg tttttttacc gttttttgtt tgattacgat gttgttctca
    15181 ccagggatgg tggccaatta tcttgttatg acaaatcttc ttcagttaaa agatacgatt
    15241 tgggcattga tcttgccaat ggctgtcagt cccttcaata ttattgtgat gcgcacattc
    15301 tttaaacgtt cggtctctga ttcgattatt gaatcggcac ggatcgatgg agctagcgaa
    15361 ctgcgaatat ttgttcagat cgttttaccg ttagctgttc caggtattgc gacaatcagt
    15421 ttatttgccg cattgggtta ctggaatgac tggttcaatg ctttactata catccaagat
    15481 gataagctag ttccactaca gtatcttttg atgaaaatcc aaagtaatat tcaatatctg
    15541 acacaaaatt caggagcagg aagccaattg acaggcggat tagcctcagt tccaggagaa
    15601 tctgccagaa tggcgatcgt agtcatctcg acgttgccta ttgcgatcag ctatccattt
    15661 ttccagaagc attttgtcaa aggtctaaca atcggcggag taaaagaata aagaaacaaa
    15721 aatataaatc ataaaattag gaggaagaaa aatgaagtta tggaagaaac tagcctttac
    15781 aggtgtttca gcgttgttgt tgggaacatt agcagcatgc ggaggttctg gttctaaaga
    15841 ccaggcagaa gctagcaatt cggacacttt gcagatgtat caaatcggag ataaaccaga
    15901 taatttcgat caattgatgg aagtagcaaa caaacgaatc gaaaaagaga tcggtgtaaa
    15961 agtcaatatc aattacattg gctggggcga ttacgaaaag aaaatgaacg tcatcatttc
    16021 ttccggggaa aattatgata tcgcctttgc gaacaactat gtttccaatg cccaaaaagg
    16081 agcctttgct gatttgactg aattagcgcc taagtacgct gaaaaagctt ataatgattt
    16141 agatgaagca tatattaaag gaaatctggt caatggaaaa ttatatgctt tcccagtcaa
    16201 tggaaatgtc tttgctcaac aagtacttac ttttaacaaa gctttactag ataaatacga
    16261 tctttcaatt gatggaatcc aatcttacgc tgatgcagaa aaagtcttga aggaattcca
    16321 tgaaaaagaa ccaaacacgg cagcttttgc cattggacaa ggcttcaaag tccaagggaa
    16381 ctttgattat cctttaggga atacgttacc atttgctatt gatctggatg gagatgattc
    16441 gaaaatcatc aaccaatacg ataatgaaac gtatatcgga ctgttacgta caatgcatag
    16501 ctggtacaaa caaggactca ttccaagtga cgctgcaacg agcaataagg attatccact
    16561 agaaggaaat acttggctaa tgcgtggaga aacacaagga ccgtatgatt atggcgatac
    16621 aattttgaca aatgcagcaa aacaagaact tgtctcaaaa gcaattactg ttccactaaa
    16681 atctacagct caagcacaaa tggcaaattt tgtcgtatca aatacttcaa aaaataaaga
    16741 aaaatcagta gaacttctag ggctgcttaa cagtgatcca gaattattga atgggcttgt
    16801 ttgggggatc gagggagaag catgggaaaa agtcgatggt tcagatgata aaatcaaatt
    16861 gctagatggt tatcagccaa atacacatat gtctgcttgg aatacaggaa acaacaagat
    16921 tctatataca caagagtcta ttacggatga catgattgcc aaacgtgatc aatcgattgc
    16981 tgatgcagaa acttcaccga ttttaggatt cagcttcaac actgacagtg tcaaaacaga
    17041 attaagtaat atttctaatg tcatgaatca atatttagat ggattgaata cagggactgt
    17101 tgatccagat gaaacattac cgaaattgaa agacgctttg gacaaggctg gctatgataa
    17161 ggtattgaaa gaaatgcaaa aacaatatga tgagttccga caagaataat aatgaataaa
    17221 gacaaagggg ttgtgacaaa agacgtgtca caacctcttg ttccgaataa acggtgttca
    17281 aaagccagtt tctcggtatt aagctaaata tatgta
//
LOCUS       DAFBQH010000059        16591 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.061, whole genome shotgun sequence.
ACCESSION   DAFBQH010000059 DAFBQH010000000
VERSION     DAFBQH010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.061"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(45..779)
                     /locus_tag="LK694_002001"
     CDS             complement(45..779)
                     /locus_tag="LK694_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBK5052847.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRFVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1105..1515
                     /locus_tag="LK694_002002"
     CDS             1105..1515
                     /locus_tag="LK694_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBK5052848.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     EGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            1508..2209
                     /locus_tag="LK694_002003"
     CDS             1508..2209
                     /locus_tag="LK694_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBK5052849.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2209..3822
                     /locus_tag="LK694_002004"
     CDS             2209..3822
                     /locus_tag="LK694_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5052850.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            3812..4033
                     /locus_tag="LK694_002005"
     CDS             3812..4033
                     /locus_tag="LK694_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBK5052851.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4030..4791
                     /locus_tag="LK694_002006"
     CDS             4030..4791
                     /locus_tag="LK694_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBK5052852.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPETFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTSGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRRKSGTIINMASIAGLVAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5172..5681
                     /locus_tag="LK694_002007"
     CDS             5172..5681
                     /locus_tag="LK694_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBK5052853.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANMASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5751..6290)
                     /locus_tag="LK694_002008"
     CDS             complement(5751..6290)
                     /locus_tag="LK694_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBK5052854.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVVVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6430..7041)
                     /gene="rpsD"
                     /locus_tag="LK694_002009"
     CDS             complement(6430..7041)
                     /gene="rpsD"
                     /locus_tag="LK694_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBK5052855.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7303..8154
                     /locus_tag="LK694_002010"
     CDS             7303..8154
                     /locus_tag="LK694_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBK5052856.1"
                     /translation="MRGHVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPAATILVEGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLAYFASQGQTDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8182..8817
                     /locus_tag="LK694_002011"
     CDS             8182..8817
                     /locus_tag="LK694_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBK5052857.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSNSEVLAFVYYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8837..9610
                     /locus_tag="LK694_002012"
     CDS             8837..9610
                     /locus_tag="LK694_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK5052858.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10018..10815
                     /locus_tag="LK694_002013"
     CDS             10018..10815
                     /locus_tag="LK694_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBK5052859.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKKIQLIQINDDRPFME"
     gene            10793..11206
                     /locus_tag="LK694_002014"
     CDS             10793..11206
                     /locus_tag="LK694_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBK5052860.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11190..13835
                     /gene="mutS"
                     /locus_tag="LK694_002015"
     CDS             11190..13835
                     /gene="mutS"
                     /locus_tag="LK694_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBK5052861.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLKKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKNSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13853..15961
                     /gene="mutL"
                     /locus_tag="LK694_002016"
     CDS             13853..15961
                     /gene="mutL"
                     /locus_tag="LK694_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBK5052862.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPVV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15983..16543
                     /gene="maf"
                     /locus_tag="LK694_002017"
     CDS             15983..16543
                     /gene="maf"
                     /locus_tag="LK694_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBK5052863.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARIVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
BASE COUNT         5388 a         2964 c         3598 g         4641 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaaaacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc gagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctaa ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 ttcacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaagaaac atcaacacga aaagcatcgg
      841 tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata aaaaaatgaa
      901 ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt aaaagttctc
      961 cacaaaccac caagcttaac tataaatata ccaaaaaaaa aacgtttttt ttctcaaaaa
     1021 ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag taaaatgaaa
     1081 gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat tattattttc
     1141 ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc tggaagtatc
     1201 ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc tgaaaaaatc
     1261 ggagcaactg gtgatttttt attgaataat ctagctattt tatttgttcc tgctggtgta
     1321 ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact gggagcagtc
     1381 gtcgtgtgtt ccatcgtcac gatggttgca gagggaaaaa cggcagaagg tgtagaagct
     1441 ttgctgggct atgtccgtaa gaaaaaagca ggaagggtat ctgtaaacga aatggaggaa
     1501 aatcaaattg actgagtttt ttgtgaatcc actatttggc ttaagtttga ctttaggagt
     1561 gtatgtatta tctgataaat ttttgcggaa atataaactg cctgttttga atcctctcgt
     1621 attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct ataaagacta
     1681 taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg cacttgccat
     1741 ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa tggctgcgat
     1801 tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact tcttctctct
     1861 tcataaagag atggtgatct cattactacc taaatctgta acgacggcta tttctgtcga
     1921 tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg tctctacagg
     1981 tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg aaagtccagt
     2041 tgctcgcggt gtggcgttag gaagtacttc tcatgctatc ggtacagcaa aagcaatcga
     2101 gattggcgaa atagaaggaa tcatttccgg acttgcgata tgcgtaaatg ggatattgac
     2161 cgttttgttg ctgccgttat tctttcagcc ttttgctggc ttgttttgat gcgaaaacca
     2221 ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa agacgtgtat
     2281 ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat tctcctccta
     2341 gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt tgttcagctg
     2401 gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca gcagtttagc
     2461 ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt ttatgatacg
     2521 ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac gctgaaattc
     2581 cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt tgcgcaaaag
     2641 cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca gcaactgcaa
     2701 acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga agaaatcatt
     2761 cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg gccatttgaa
     2821 acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt ttttaatgtt
     2881 cgtctgaacg agatcaaaaa aagacgattg gaatacatgc taggtgcctt ttttttaaga
     2941 aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat ttctgacaat
     3001 ctgttgttcc agcgattttg ccaagtcatg gagccggtat ttcctaatta ttttcaagta
     3061 gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata ttatagtgat
     3121 cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc gccctttaca
     3181 gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg gctgccagca
     3241 gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt cgcctatctt
     3301 tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa tcatttagtt
     3361 attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag acacaaacat
     3421 cccaatcatc tggcatttaa aaatcatgcg tttttgatgg gtcggtatct gactgtgttt
     3481 aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt aatgacagat
     3541 ttcccattat ttgaagagca actgttagag gagggattac ggacattttt ccgtaatgag
     3601 tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct tctgatttcg
     3661 acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt gaccgaagaa
     3721 ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca aaaaaaaact
     3781 attaaaacaa taacaaagaa ggagaaaaaa gatgacattt gaagaaattt taccagaatt
     3841 gaaaaaaggt gcaaagatta ttcgtaaagg atggagtgga tttgaacttt atgttgttct
     3901 cgtatccgat gatgaatttg atggctgtcc agtgacacct tacttattga tcaaaacaag
     3961 tgacgaaggt ttttccagct tcgctcctac agtttgtgat attttagcag atgattgggc
     4021 gatcgtagaa tgaaaaacca gtatccagat ctagctaaac aggttgtatt tatcacagga
     4081 gctgcttctg gaattggtgc tgcgcaagcc cgagcgtttc ttgaacaaga tgcttttgta
     4141 tttggattag atcaaaagtt cggtaaaatg gaagaactct ctttgcgatt tcctgaaact
     4201 tttgcctacg cactaggtga tgttcgacaa atgaaagaac tccaacaagc ggtatcgaaa
     4261 tgccaggatc gttttggtga agttacgatt ttactgaata catcggggat attggatgcc
     4321 tacaaaccat tgatggaaac tgacgaatcg ctgtgggatc ttatttatga gacgaatgtg
     4381 aaaagcatgt atcagctgac aaaaatcatc cttcctgata tgatccgccg aaaaagcgga
     4441 acaatcatca acatggcttc gattgcgggt ttggttgctg gcggaggtgg gatcgcttat
     4501 acaagtgcga aacatgcaat cgtcggattt accaaacaac tcgctctgga ttacgcagga
     4561 cagggtatcc gagtaaaagg aattgctcca ggagccatcc aaacaccaat gaatgcggct
     4621 gattttgaag ggaaaggtga gatggctgag tgggtcgcaa atgaaacacc agtcaagcga
     4681 tgggcacagc cggaagaagt agctgagctg acgttatttc ttgcctctcc tcaagcaagc
     4741 tacatacaag gaacgatcat cccaatcgat ggcggatggt tgttaaaata accttgtgtt
     4801 tttttcaagc ttctgtatga taaaactaaa gagagaaaca acggttgatg ataaaagaaa
     4861 gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt ctttttaata
     4921 atcgttaata ttcgaaacta agcggctttt cacgaccttt tgatataaac ggtgttcaaa
     4981 agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct atttttattt
     5041 gactattctt attacttgtt gcagggcagc tttttcacta ccttctgcgg cacttcagtt
     5101 ggaattttct tcccatcctt tggtcgcagt cattaccaag ataaaattat cttgcaaagg
     5161 agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca acgacagatg
     5221 tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt ttgtctgtct
     5281 ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg tccttgtata
     5341 acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatg gcttctcctc
     5401 ttggactgat agatgttgta gtaggaagcg tcagcacgct gcttgtattg cttatcaact
     5461 ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggctgtgaca gcatgcgtat
     5521 ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa ctaccttttt
     5581 tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc ggtgggataa
     5641 ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt taatttaaaa
     5701 tatggcagcg tggaagagac tttgagtctt cttccatgct gcttttttga ttaatttgta
     5761 tgaagaaatc gatttagccg ttttttcatc aagataccga tataagctgc tccagttgcc
     5821 ttgattgctt ccggcagtaa aaacggcagt agtccactgg caaatgcagt tgtaaaagta
     5881 agatcaccac ttattttcag ccagatcgtt ccgcagatca atgcagcaag agagcccatt
     5941 agattggcaa agattgcata taagtaatga gcatccgttc tttcgatcat ccaaccagtt
     6001 atccacgttt gtaagagaaa tccaatcaaa tatccgccag ttggcccaaa taaaacagct
     6061 atacctccag tcatccctga aaatacagga agaccaatca agccaagtaa aagataaact
     6121 acaacagata tcaatcctag ttttcttccg agaaaagttg ctgttaatcc aatagcaaat
     6181 gtttgccccg tcaaaggaac caagccaaaa ggaatggtca tctgagaaag gacagaaata
     6241 atcgcggcaa attccgcagc aagtattaat tctttcaatg ttcggttcat aatgccctct
     6301 tttcgttaac ttaatatttt tatttggtta acgaaattgt aaaacaaagt gattactttg
     6361 taaatagaaa atataaaaaa agaccagcta tccaatagat agcggtcttt ttcaagaaag
     6421 cttttgaaat tataagcttt ggttgtagta ttcaacgaca agagcttcgt cgatttctgg
     6481 atatagttcg tcacgttctg gtaaacgagt caatgaacct tccaattttt cagcgtcaaa
     6541 gcttacgaat gctgggcgtc caacagttgc ttctacagct tcttttactg ttacgatgtt
     6601 ttgtgatttt tcacgaactg agatcacttg acccacttct acgtggtatg aagggatatc
     6661 aacgcgtttg ccatcaacta aaatgtgacc atggttaact aattgacgtg cttgacgacg
     6721 agtcgttgca agtcctaaac ggtaaacaac attgtctaaa cgttgttcta gtaagatcat
     6781 gaagttaaca ccatgtttac cttctttgat tttgctagct tttacgaaca agttacggaa
     6841 ttgacgttcg ttcattccgt acatgtgacg aagtttttgt ttttcagtta attgcatgcc
     6901 gtattctgat tgtttgccac ggctgttagg tccatgttga cctggtgcgt aagggcgacg
     6961 tgctaattct tttcctgtgc ctgacaatga gatgcctaag cgacgagaaa ttttccatga
     7021 tggtcctgta taacgagaca ttaaaatttc ctccaatgaa tataagtttt tggagtaaaa
     7081 taatccgttg aaaaattcat aatcatatag tttgttcttc aatcttcacc tttgcagccg
     7141 cggttacaca atcgaacccg agacggggca acaaactagg gatgagttat tatttttctg
     7201 ctgcattatt ttacacaaac tacagtatat gatagatcat ccacgaatgt caagataatt
     7261 agtacataaa aacgctttac aaacctaggc tgtctttata aaatgagagg gcatgtgcaa
     7321 atggaaaaaa gcgaaggaag tttaaggaga aaatcaatga atccggtatc accattattt
     7381 gagaaaatcg atgcttcaat tgaaaagaag gcgaatcttt ttgaaaacag cttttcacgt
     7441 tacgctgttc gagcggttct cgcctgtttg tttttgactt taggaacggc tgtggcattt
     7501 gcaatcgcga tgaaggggga agatatcgcg catggtctag gaaagatttt atacgcattt
     7561 atgttcagct ggggtctggt catgattcta tacatgaacg ctgaattagg aacatcgaat
     7621 atgctttata tgaccgtggg tgtttttcgg cagagacttg ctttaagtaa ggcagccaag
     7681 attctatttg cctgtatttt tttcaatatg attggcggta tattatgtgg atttcttatt
     7741 tctatgacta cgccatttca agatttaacg ttggacaact ttatgttaga atcaatagca
     7801 gtaaaactag caaaacctgc agcaacgatt ttggtggaag ggatgtttgc gaatatcgtt
     7861 gtcaataccg ctgtactgat cagcatgcgg atgaaagagg atgccgggaa agtgatcaca
     7921 gtcgtattca tcattttcat ttttgctttt ttaggatatg aacacgtgat agcgaatttt
     7981 cctgccttta cgcttgctta ctttgcttct caaggacaga cggatggaat gacagtcagc
     8041 aatgtgttac ataatttgtt ttttgctttg gcaggaaatt atattggtgg cggtttagta
     8101 atgggactgg gatatgcttg gctgaatcag tcaaaatcat cctatgtgga ctaaaaagtc
     8161 gttcattaaa ggggaagtgc catggaagtt ttagtttttc caaaaaatga ggaaaaagcg
     8221 ttacaaatgg aaaagtacat gaaaaatctt ttttcctttg ctggagtacc taagccggaa
     8281 cgcgctcttt tacaacaacc gttactggat gagagcaaaa atttatctaa ttctgaagtt
     8341 ttagcttttg tttattatta ttatgaacaa atcgaaagag agtatcagta tgtagcgatc
     8401 gatttagtcc agaaaaacat caaacggctt acatttgaag acttagtcgt attgttgcca
     8461 ctagttcaga aaaaagaatg gtgggacagc atcgactctt ggagaaaagt atacagcaca
     8521 tggtgtaaag cctacccaga gcagcgtgaa gaagtctttc aattgtttga aaaaaaggaa
     8581 gatttttggc tacgtcgaat agcaatcacg ttgcaattag gttataaaca gcaaacagac
     8641 acaggactat tgatccgagc aatagacgaa gatcgtcaaa caaaggaatt ctttatccaa
     8701 aaagcaattg gttgggcatt acgcgattac agtaagaccg atgccgattg ggtaaaaaga
     8761 cagctgacaa cagatttgag taagttagct gttcgagagg caagtaagta tttataagaa
     8821 agcgagacga caagagatga gacgcaaatt atttgctttt gatattgatg gaacgttgtt
     8881 agattcaaac aaacaagctt tagatagcac gagagaagca ttggaaaaat tacgcaaagc
     8941 cggtcatctg gtcacgattg ctacagggcg gagccgattt catgcgcaag agatcattcg
     9001 cgatcttgat tttacgaact acattttatg taatggagcg gccggttttt tagaccatga
     9061 acaagtatat aagaatttac tagatcgcga ccagttggat gcgttcgttc aagaagcgca
     9121 tcatgagcag atcgatacag cttttgtcag cttagacaat atcaagcgtt cttcttccaa
     9181 tgatgtaacg gtgatggaag aagcgatgca ttctttcggt gctgtcttgc ctgaattaga
     9241 tcagtttttt gcagaggaac aagaaatata ccaagcgtta gcatttttcg atcaaacata
     9301 tgatgggaag ttctcttcat atgataaaat tcgatttgtc cgctggcatg agaacagtgt
     9361 agatgtcgta cctaaaaatg gttctaaagc agcgacaatt ctgcacttag cagaacaagt
     9421 aggcatccct catgaagaca tcatcagctt tggcgatgga caaaacgacc gtgaaatgtt
     9481 gcgcatgtca ggaattggga tagctatggg gaatgctgtg ccagaagtac aagcagaagc
     9541 taagatggtc actgatacaa acgatcagga tggaatttgg aaggcattga aagagttaag
     9601 cgtgatttaa aagaggttgt gaaaaagctg ttctgcatca agtaataagc ccagccaaac
     9661 aaaaatagct cctcctattt ttcgtttttc tgggcttatt accgcagatg caagcttttg
     9721 aacaccgttt aaggaagagg tcgtgacaga agtggtcagt ttcaagaaat aagaagggat
     9781 ttacgaaaat tgcttctcaa atttttgtaa aattacggct tatttctgaa gaagtagatg
     9841 aaacaacgtt tattcgggaa atgggactgt gacaagatgt ttgtcatagt cccatttttt
     9901 tattttattt ttcaaaacta agaaatacca ggaaagagtt ccttttgttt gaccttttct
     9961 ttcttattga aaacaactat aatatagagt gatgttaaaa gagaagagga atttagtgtg
    10021 cgggtattgt ttattggtga tgtagtaggt tcaatgggac gagagatgat tactgaatat
    10081 cttcctagat tgaagaagaa gtaccgtccg caagtaacga ttgtaaatgg tgagaatgcc
    10141 gcttctggac gtggaatcac agaaaaaatc tataaaaaat ttttgcaaga cggcgtagat
    10201 gtcgtgacga tggggaatca tacgtgggac aaccgcggaa tctttgagtt tgtaaatgaa
    10261 gctaaaaaga tggttcgtcc ggcaaatttt ccggaaggga cgcctggaca agggatggtc
    10321 tttgtcaaag tgaaccaagt cgaactggca gtgatcaaca tgcaggctcg ttcttttatg
    10381 gtggacttgg atgatccttt tcgtaaaatg aatgagctgg tagaagaagc cagaaaacgt
    10441 acgccgatca tttttgtaga ttttcatgga gaaacaacaa gtgaaaaaca agcaatgggc
    10501 tggtttttag atgggaaagt ttcagcagtg gtcggtacac atacacacgt tcagacaaat
    10561 gatgcgcgaa ttttgccaag aggaacagcg tatctgacag acgttgggat gacaggtccc
    10621 tatgatggta ttttgggtat gcgcagagaa cctgtaatcg aaaagtttct gaccgcatta
    10681 ccaaaacgtt ttgaagttgt ggaacaaggc cgatcgattt taagcggctg tatcttggaa
    10741 ttggatgata cgacaggtca tgcaaaaaaa atccagctga ttcagatcaa cgatgatcga
    10801 ccgttcatgg agtagaaaga gaggcagttt tttgcagcag gagccggaaa ttcaaaaaga
    10861 agtaaggaaa ctgacaaaac ttttaagaga gaatgaaacg atcattcgct acaaagaact
    10921 tgaagaaaag atccagcaaa accagtattt agctgagttg agagaaaaaa tcaaacaagc
    10981 gcaaaaagat gctgttcatt ttgcccacta tgacaaacca gcagcagaac aagaagcaat
    11041 caaacaagcg gatcaattca tgcaagaatt tgatcagcat ccgcttgttg tagcttatag
    11101 aaaacagctg ttggaagcag atgatctgct tcatcattta acaactatga tacaagaaga
    11161 aatcaatgga caaatagagg aggaaaaaca tgcctcaaaa aactaaaaat actcctatga
    11221 tggaacaata tctaagcatc aaggcccaat accaagatgc ttttttgttc tatcgtttag
    11281 gagactttta tgaactattt tacgaagatg cgatcaaagc gtcacagctt ttggaattaa
    11341 cgttgacgag cagaaatcga aatgcagaag aacctattcc tatgtgcggt gtacctcacc
    11401 atgcagcaca aggatatatc gatgccttga ttgaaaaagg ctataaagta gcgatttgcg
    11461 agcaggtaga agatccaaag acgactaaag ggatggtcaa gcgcgaagtg atccagctag
    11521 tcactcctgg tacagtcatg gacagcaaag gattgtcggc aaaagacaac aattttttaa
    11581 cagctgtagt tagtcaagga aatgaatttg gctttgctta tgccgattta agtacgggag
    11641 aattgaagac ggctcggttg catgatgaag aagctgtttt aaatgaagcc tctgcattgc
    11701 agacaaaaga actcgtatta ggcagtgaga tcactgatac ccttcgtgaa cagctaagcg
    11761 ggcgtttagg gctagtcttt tctcaacaaa atgagatgga agaaaacgcg gaatttagct
    11821 ttttgacaag cgaactagtc gatccgctga aaaaagaagc aaccggcaaa ctattgactt
    11881 atttatcggt gacacaaaag cggagtttag ctcatatcca aaaagcagta gaataccaac
    11941 cagatcattt tttgaaaatg gattattatt caaaattcaa cctagaatta agccaatcta
    12001 ttcggactgg gaaaaagcac ggcactcttc tatggttgtt agatgaaaca aaaacagcaa
    12061 tgggtggccg tttactaaaa caatggctag accgtccgct tatccaggca aaacaaatta
    12121 aagcaagaca agaaatggtc gcttctttgt tagacagcta ttttgaacgt atcgatttgc
    12181 agtcagcatt aacgaaagtc tatgatctgg aacgcctagc aggtcgagta gctttcggta
    12241 atgtcaatgg gcgagacttg atccagctga aaaattcttt agaacaagtg ccaatgatca
    12301 gagggttgat tgaaggaatc gatcaaggtc aatggcaaag tctgctttca gaaatgcagc
    12361 cgatggacca tctcgtccaa ttgatcgacc aagctatcca agatgatccg ccgttacaaa
    12421 taacggaagg aaacatcatc aaagatggat tcaatgagca gctcgatatg taccgttctg
    12481 ctatgagaaa cggaaagaaa tggttggcag aacttgaagc aaaagagcgt caagaaacag
    12541 gaatcaaaaa cttaaaaatc ggatttaacc gcgttttcgg ttattatata gaaatcacaa
    12601 aagcaaatct tggaaatgca gaactggaga agtatgaacg aaaacaaact ttagcaaatg
    12661 cagagcgttt catcacacca gaactaaaag aattggagac acaaatcctc gaagcagaag
    12721 aaaaatctgt tgatttagaa tatcagttgt ttttagctgt gagagaagaa gtcaaaaagg
    12781 ctatccagcc gctacaagta cttgcaaaag caatcagtgc agtagatgtg ctacaaagtt
    12841 ttgcaacgat cagcgaacgc tatcagtatg ttcggccaga acttgtcagt gacaagcatc
    12901 agcttctgat caaagacgga cgacatcctg tagtagaaaa agtattagga catcaggaat
    12961 atatccctaa cagcgtagaa atggcagaag acgaaatgat cttgctgatt acaggaccga
    13021 atatgtctgg taaaagtacg tatatgcgac agttggcatt aactgttctg atggcgcaaa
    13081 tgggctgttt cgttccggca aaacaagcag ttttacctat ttttgatcgg atttttaccc
    13141 gaattggtgc aagtgacgac ttaattgctg gacaaagtac ttttatggta gagatgatgg
    13201 aagccaatca agccctgcgt tatgctacgc caaacagttt gattttgttt gacgaactag
    13261 ggcgaggaac agcaacctat gacggaatgg cgttggccca agcaatcatc gaatatatcc
    13321 atcgtcatgt acaagcaaaa acactctttt ctactcatta tcatgaactg acggtacttg
    13381 agaaagaact tccgcagctt aaaaatgtcc atgttggagc cgttgaaaaa gacggagaag
    13441 ttgtcttctt gcataaaatg atggatggac ctgcggataa aagctacggc atacatgtgg
    13501 caaagatcgc aggattgcct tccaatctac tagaacgagc agcagatatc ctgcatacgc
    13561 tagaaaatgg cgataacgga caccatactg ttccagctga agtagaagag aaaactaaaa
    13621 cagccaaaca ggagcaaata aaaccacctg cagaaaaagt aaatcaagac catatacaag
    13681 accgtgtaaa agaagaaaac caacagcttt ctttattcag tgaattatcc gaaaatgaaa
    13741 cagaagtcat tgaatcactg aagcaaataa atttactaga aatgacgcca atggatgcac
    13801 taaacacatt atatgaatta caaaaacaac tgtaggaaga aggcggatat cgatggccaa
    13861 aatccaagaa ttatctgaac gtttagctaa tcagatcgct gcgggagaag tagtggagcg
    13921 accagcttcc gttgtgaaag aattagtgga aaatgcaatt gacgccggca gtactcagat
    13981 tgatatctta gttgaagaag cgggactgcg gacgatccaa gtgattgata acggagaagg
    14041 cattttggca gacgacgtag agaatgcctt caagcggcat gcgacaagta agatccataa
    14101 tcgcgacgat ctttttcgca tacgaacgct gggctttcga ggggaagctt tgccaagtat
    14161 tgcctctgtt tctgaaatga ctctggaaac ggcgaatcag gaagaaaaac aaggcaccta
    14221 tctgtcgctg aaaggcggag aagttattga gcatcgacca gcaccactgc gtcaaggaac
    14281 gaagattacc gtcagcaatc tgtttttcaa tacgccagcc cgcttgaaat acgtgaaaac
    14341 actgcacaca gaattagcga acatcgggga catcgtcaat cgactggcat tgagtcatcc
    14401 atcgattgct tttcgtttag tacatgatgg aaacaaaatg ctggcaactg ctggaaatgg
    14461 cgatctgaaa caaacaattg cgggaatcta cggattatct accgcaaaaa aaatgctgaa
    14521 aatcgaagga aaagatctag attttgagtt atttggctat gtttcattgc ctgaggtgac
    14581 tcgtgccagt aggaattact tatcgacgat catcaatgga cgttttatca agaacttttc
    14641 cttgaataaa gcaatcgttg caggttatgg gtcaaaatta atggtcggtc gtttccctat
    14701 tgctgtattg gaaatcaaaa tggacccgct tttagtagat gtaaatgttc atccgacgaa
    14761 gcaggaagtc cggctgtcga aggaaaaaga actaacagag ctaattagta acgccattca
    14821 aaaagcattg tctcaagaaa atctgattcc aagtgcagcg gataatctac ggtttaagaa
    14881 aaaagtacct gatcagccta aaacagaaca aatcgaaatc gatttgtcct acgcttttga
    14941 agaagaaaaa cctaaaaagc caagtagttt gaattttgat cgagagacag gtaccttttt
    15001 cgtagaagaa ccaaagaaca catttgttga cagaattgtt gataagtctg tggaaaatgt
    15061 tgaaaactat tctggttttt ctgaagaaaa caccacttat ccagaaaaaa cagcgaaaac
    15121 ggtggataag tttggtgaaa atgttgataa ctttgtagaa aaaacagtgg aaaaatctga
    15181 aaagaaagaa tttcccgttg tggataacat ggagaaaaca aatgaagaac aagcagataa
    15241 atccgtagaa gaggatgaaa agatttacga ggaagaattg acactccatc ctgaatttga
    15301 tttcactcaa ggaaacgaca aaacattaga acaagccatc aagaaattag aaacggaaaa
    15361 accagtacaa cgttttcctc agttggaata ttttggacag atgcacggaa cgtatctttt
    15421 tgcccaaagt agcgatgggt tgtacatcat tgatcagcat gctgcccaag agcgcattaa
    15481 gtatgaatat ttccgggaaa aaattgggga agtatctgat gatctgcaag aattgcttgt
    15541 tccatttgtg ttagactatc caaacagtga tgctttgaaa ttaaaagagc aaaaacactt
    15601 gctagaagat gcagggattt atttagaaga atttggtcaa aacagcttta ttgtacgggc
    15661 ccatcctact tggtatccca atggagaaga agaatcgatt attcgggaga tgatcgatat
    15721 gctgctgaca actggaagtg tgagtgtaaa aaaattccgt gaagcaacag caatcatgat
    15781 gagttgcaaa cgatcgatta aagccaacca ttatttgaat gaagcacaag cacgtgtgct
    15841 gctgaaggat ctggcacgat gcgaaaatcc tttcaattgt ccccatggcc gtccagtatt
    15901 gattcacttt accaattcgg atatggaaag gatgttcaaa cgaatccaag acccgcatta
    15961 agtcaaagag aagagggaaa acatgaagat tgtattagca tcccaatcac caagaagaaa
    16021 agagttattg gctcatatcg tttcagaatt cgagattgaa cctgctgata tcgatgaaac
    16081 gccatttcca gacgaacagc ccgtcgatta tgttcgtaga atggctgaag aaaaagcacg
    16141 aattgtctgg gaacaagatg aaaaagaaga ccatttagtg attgccagtg acacgactgt
    16201 tgttctcaat caagagatca tgggtaaacc agaagatctg gctgaggctg aattcatgct
    16261 gaaaaaacta agcggggaaa cacatactgt atatacagct gttgttttaa aaacaaagga
    16321 acgagaagaa cggatactag cagaagcaca tgtcactttt tatcctttga cagacgaaga
    16381 gatcaaacgc tacttggaga cgggggatta tgcggataag gcaggggcat acggcatcca
    16441 aaatgctgcg agtgtattcg tcaaagaaat ttctggagat tattacagta tcgtaggatt
    16501 tccgattggt gctgtcaatc aggcactgaa aacattcatt taaacaaaaa agcggactat
    16561 ctgattttcg attgaaaatc catgatagcc c
//
LOCUS       DAFBQH010000060        16523 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.062, whole genome shotgun sequence.
ACCESSION   DAFBQH010000060 DAFBQH010000000
VERSION     DAFBQH010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.062"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(137..412)
                     /locus_tag="LK694_002018"
     CDS             complement(137..412)
                     /locus_tag="LK694_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBK5052864.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(655..1965)
                     /gene="der"
                     /locus_tag="LK694_002019"
     CDS             complement(655..1965)
                     /gene="der"
                     /locus_tag="LK694_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBK5052865.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2147..3376)
                     /gene="rpsA"
                     /locus_tag="LK694_002020"
     CDS             complement(2147..3376)
                     /gene="rpsA"
                     /locus_tag="LK694_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBK5052866.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3511..4191)
                     /locus_tag="LK694_002021"
     CDS             complement(3511..4191)
                     /locus_tag="LK694_002021"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBK5052867.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4240..4875)
                     /locus_tag="LK694_002022"
     CDS             complement(4240..4875)
                     /locus_tag="LK694_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK5052868.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4941..6344)
                     /locus_tag="LK694_002023"
     CDS             complement(4941..6344)
                     /locus_tag="LK694_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBK5052869.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6331..7362)
                     /locus_tag="LK694_002024"
     CDS             complement(6331..7362)
                     /locus_tag="LK694_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5052870.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7393..7614
                     /locus_tag="LK694_002025"
     CDS             7393..7614
                     /locus_tag="LK694_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBK5052871.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7779..8516)
                     /locus_tag="LK694_002026"
     CDS             complement(7779..8516)
                     /locus_tag="LK694_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBK5052872.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8669..10009)
                     /locus_tag="LK694_002027"
     CDS             complement(8669..10009)
                     /locus_tag="LK694_002027"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBK5052873.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10246..11769)
                     /locus_tag="LK694_002028"
     CDS             complement(10246..11769)
                     /locus_tag="LK694_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBK5052874.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12147..12758)
                     /locus_tag="LK694_002029"
     CDS             complement(12147..12758)
                     /locus_tag="LK694_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK5052875.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12881..13006)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13088..13804)
                     /locus_tag="LK694_002030"
     CDS             complement(13088..13804)
                     /locus_tag="LK694_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBK5052876.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13810..14379)
                     /gene="scpB"
                     /locus_tag="LK694_002031"
     CDS             complement(13810..14379)
                     /gene="scpB"
                     /locus_tag="LK694_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBK5052877.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14363..15160)
                     /locus_tag="LK694_002032"
     CDS             complement(14363..15160)
                     /locus_tag="LK694_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBK5052878.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15130..15501)
                     /locus_tag="LK694_002033"
     CDS             complement(15130..15501)
                     /locus_tag="LK694_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBK5052879.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15520..16407)
                     /gene="xerD"
                     /locus_tag="LK694_002034"
     CDS             complement(15520..16407)
                     /gene="xerD"
                     /locus_tag="LK694_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBK5052880.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4641 a         3577 c         2798 g         5507 t
ORIGIN      
        1 attaaataaa atacatgtgt tttctatgta tgacatacgg attcggaatc tctgtctctt
       61 atttattcct tttctgctag gaaataaaaa gaaaagcact tgtagcccta gagccacaag
      121 cgctttttca actgtcttat ttaacagcgt cttttaatgc tttacctggt ttgaatgcag
      181 gtactttgct agctggaatt tcgatttctt caccagtttg tgggttacga cctttgcgag
      241 ctgcgcgttc gcgaacttca aagttaccaa aaccgattaa ttgaactttt tcgccgttag
      301 ctaaagcatc ttgaatagtt gaaaatacag catctactgc tgcagttgca tcttttttag
      361 ttaaatcagt agctgaagca actttttcga ttaattctgc tttgtttgcc atggatagat
      421 tcacctcctc cacaagagat atgatagaaa tatcatttag ttaaaacttt ctgagtggtc
      481 cagaagttta aaaagaaata ttgattaact tcaatattct gttatcacga tatcatatga
      541 accgcttaat agcaaggttt ttaccaattt tcacatgctt tttttagtca tagaaacgta
      601 acaaacgaaa ctgtcttttt cttttgaaaa aaaaacgtcg atatggtaaa atgcctattt
      661 tctcctacga gggataattc ggatggacgt cccttcaaaa gtaaatgctt ttctgatttg
      721 attttctaag aaacgttcat aagaaaaatg catcaattcc tcttcattga caaaaacgac
      781 aaatgtaggt ggtttgacag cgacttgtgt agcataaaaa atcttcaggc gttttccttt
      841 atctgttggt gttggattga ttgctacagc atccataatg atatcattca acacagccga
      901 agagatacga agattctggt tcatacttac ttgttcgatc atttcaggca gacgttccaa
      961 ccgttgttta gtcactgcag aaacaaaaac gattggagcg taatcaagat accggaattc
     1021 atcacgaatc tcttgttcga aatcacgcat tgtattggtt tctttcttta caagatccca
     1081 tttattcacg acgataataa ttccacgacc agcttcatgg gcgtaacccg ctactcgctt
     1141 gtcttgttcc cgaatccctt cttcagcatt cagtaccatc aaaacgatat cagaacggtc
     1201 aatggcacgc attgcacgca tcacgctgta tttttcagta ttttcgtata cttttccgcg
     1261 tttacgcata ccagctgtat ctatcattaa gaatttctga ccactttcag aaacgaagtg
     1321 ggtgtcgatt gcgtctcggg tcgttccttc aatatccgaa acaattacac gttcttctcc
     1381 taaaatcgca ttgatcaaag aggatttacc aacatttggg cgaccaatca aactaaactt
     1441 aatcgtgtcg tcgtcctctt cttcagtctc ttcagagaaa tacttcactg cttcatctaa
     1501 aatatcacca atccctagtc catggcttcc tgaaacagga taaggatcgc ctagccctaa
     1561 ggaataaaat tcatagatat cgtttctcat ttctggatta tctactttat tgactgctaa
     1621 aatcactggt ttattacttc tgtataaaat tttcgcaacc atctcatctg cgtctgtcac
     1681 gccttcgcgt ccgctgacta cacaaatgat tacatctgct tcttcaatcg cgatttctgc
     1741 ttgatgcttg atctgatcca taaaaggttc atcgccaaga tcaattccgc ctgtgtcgat
     1801 cacgctgaat tctcgaccta accattcacc ttttgcataa atacgatcac gagttactcc
     1861 aggagtatct tcgacaatcg agatacgctc accagcgatg cggttaaaaa tcgtagactt
     1921 accgacgttc gggcggccaa cgattgcaat tgttggattt gccatattat ttcctcctca
     1981 caagtttcca atctcactta gtttaacgaa tgcattgcca actttcaagc atagattgtc
     2041 aattaaacga agtattttta ttccttattg aaatactaat cgcttttcaa aagaaaaact
     2101 gagacaaaag cagctgcttt catctcagtc acagatacaa aatcaattat ttttcagaat
     2161 catcggttgc tttttcttct acttcttctt tcaacgcatc acctaaaatg tctcccatag
     2221 taaatccagt attttcttct ggtaattcat aagactcaac attttttggt tcttcttctg
     2281 aagctggttt ttcttctaaa gctttgatac tcaatgcaat acgatgttct tctggatgga
     2341 tctctaatac tttcactttc acttcgtcac cttcatgaag cacttcatgt ggtgtcgcaa
     2401 tatgtttatg tgaaatttga gagatatgga ctagcccttc tactccaggg aacacttcaa
     2461 caaaggcacc gaagcttgtc aaacgtttaa ctgttccgtc taatactgtg ccaactggag
     2521 cttttgtatc gatttctgac caaggtccag gctgtgtgtc tttgattgac aaggaaacac
     2581 gttcttgttc aggattgata gacaacactt tgacatttac atcatcattg acagccaata
     2641 catcacttgg ttttgctaca tgactatgtg aaatctctga gacatggact agaccgtcaa
     2701 ttccgcctag gtcgacaaat gcgccaaaat cagtaagacg tgcaacacgt ccactgagaa
     2761 catctccctc atggatagtt gaaagaattt cttttttctt ttcttctctt tcgttttcca
     2821 ctaccgcttt atgcgataag atcaaacgat tttctgatgg ttcgatttcg atgattttaa
     2881 agctcaatgt tttgccttta tagtctgaaa agtctgcaac aaaatgatct tccaccattg
     2941 atgcagggac aaaaccacgt acaccgacgt caacgactaa accgcctttg acaacatttg
     3001 taacaggtgc ttcgatgacc ttgccctctt ggaaatctct ttcgatatct tcccaaactt
     3061 ttttagcatc taaacggcgt tttgaaagta ggtagctgcc gttttcttta tctttgccga
     3121 tcgtactgat aacgactaaa tctaatacat caccgacttt tactacttcg ctgatatctt
     3181 ctaccggcaa agttgataat tcttttgccg gaacgactcc ttcaactcca gccccttcga
     3241 ttccgacaat cacttgtttg tcttcaatga ctaaaacttc gcccttaaca acatcgccaa
     3301 cttttacttc ttgtacgctt ttcattgcgt cttccattga ctggttgttc tcttgatttt
     3361 gatcaaattc tgtcataaca cttgtcctcc taaccaacat tctgccgtaa gtacggtact
     3421 gtttttagtt taattgatta ttcaaaaaaa ataaagcgat ttcgttcgtt ttccttgaaa
     3481 aaacaattgt ttttcatttt ttttcttaat ttaaaaaaga agataacctt ttttcaaaac
     3541 aacagctttt attgctgcaa cgacttctgg aatcgatttg ccagtcgtat cgatacgaac
     3601 agcatcctct gcttgcttca gcggagagac gtcccttgtt gaatcataat ggtctctttc
     3661 ctgtatctct ttcgtcagtg tttcaagatc agtcggaatt cctttttctt ggttctcttt
     3721 gaaacgtctt tctgcacgct cacttacgct tgctactaaa aagattttca cttctgcatt
     3781 cggtaataca gctgtcccga tgtctcttcc gtccatgaca acgccaccgc gttcccctat
     3841 ttttcgttgg acagcaacta attcttctct gacttttcca tgtgcagaga cttcagaaac
     3901 agcttttgtg acatctggtt gacggatttc taaagtcaca tcttttgatg ctatgaatac
     3961 atgctgtcca tcttcttgtt gcgaaaaaga gataggatac tttttgatca gcgaaactag
     4021 cccttcttca tctgaaaaag atacattgtt cgtaatagct aaataagtta ctgcgcgata
     4081 catggcaccg gtatctgtat aaatataatt caattctttt gctaatatct tggcaacggt
     4141 acttttccca gatgaagcag gcccgtcgat tgcaatactg atttgtgtca tgatataact
     4201 cctttttctc agaaaaaaag ccgcaaagcg cggctattat tatttaattc ttaattcttg
     4261 accagggtaa agcataaagt tgtttggatc taagccattc aattcaaaca attgatcagt
     4321 ggtgatccct gcacgctcag ccacttgttt tggaccttcg cctgcttgaa cagtcgttgt
     4381 tgaaccggct gcagctgatg aagaaggtgt tgtttctgct gcattgctgc ttgaatcccc
     4441 tgcttgataa gttgcctcac ttgaggaagt cggtgttgtc tctgctgcaa tactgctttc
     4501 tgacgttaca cctgtatctt gagacacagt agattcttcc gcgattgtac tagattctac
     4561 tgttgaagtc tcagttgact cctcttttgt tgtacttgta gaactttcta ctactgatgt
     4621 cgaagtttca gaagaagttg ctgctgttgc attgttgctt gatccatttc tgatccagaa
     4681 ataagcacct gctggaatag cgatacataa gaccagtaaa atcaaaaata gcgaaagaaa
     4741 taatgtattt cctttctttt gttgtctctg agaacttctt gatgaaactt catcatctgt
     4801 atcgtagatt ggttgttccc atggttcttg tgtttcgtta ttttctgtag attgatgtct
     4861 atcttttctg ctcactgttt tacctcctaa actctatcgt gtgtattata ccataggaaa
     4921 aagtgtcttg tcctaaaaaa tcaatgttcc atgttttttt taaataaatt tagtaaaatc
     4981 gtttcccaat gaagttgttc agtcttagaa aaaagcttcc tctttggcag aagttctgct
     5041 tttgctccat caatatcaca acaattctgc ggcttttcgg agagtttttc gccaaaataa
     5101 gctaagatga attttctgcg gcagttttct tcattaatat agcgaagcat ctgctgcagg
     5161 cggaattctt tttctttttc ctgacgtttg agccgttcca cccaattttc tggtttttca
     5221 gactgctgga tcagctccat ccattttttt tgcagctcat caaagggagc ttgctttgtg
     5281 gcatattcca aataaagctc aaaactttgt cgctgttctc tagaaagttg attaaagaaa
     5341 taatggatac gttcatcccc actttgataa aaaagaattg ctgcactttc ttcctgatct
     5401 ctgcctgctc gaccgatttc ttgtacatag ttttctagac tgtctggcaa atcataatgg
     5461 accacataac ggatatcaga tttatcgatc cccataccaa atgcattcgt agcaatgaga
     5521 aactgcagct gattttttac aaactgctgt tgaagttgtc ttctttgagc tgcatcaaga
     5581 ccaccgtgat agtatccaac tgtaaaacgt tccctgaata aatgatacaa tcgttcgact
     5641 tctttttttg ttgcacaata aatgattgcc gctccttgtg ctctttccat gaagtgttca
     5701 agttcttgtt ctttttgctg cgttttccga acaaaaagcg agatattttg ccgattgact
     5761 gattgtctga tctcttttgg tggtcgatca aatagtacat gacgtatatc tcttgctact
     5821 aaatctgttg ctgtcgccgt tagtgccagt gtgaccgggt ttcctaaaag ctttcggatt
     5881 tttcctaact gttgatattc tggacggaaa tcaactcccc attgagacac gcaatgagcc
     5941 tcatctacta catataacgc aatattttgt tttttcagat gctctaaaag agaaggatta
     6001 gtcaacattt cgggactcaa aaacaaaaat ttatattgcg atagatgacg gaggacgtat
     6061 cttctttcag attgcgaaag taaactattg aaagcagcta ctcgtttctc ccctcgtttt
     6121 tgtaattgcg tcacttgatc ttccattaga gaaagcagcg gtgacacgat gatgaccaga
     6181 ccttccatta aataccccgt caattgatag caaaggcttt ttcccgttcc tgtaggcaaa
     6241 atagcgagag tatcttctcc atcaagcaac gcctgaataa tttcttcttg gccgggacga
     6301 aatgaatcaa atccaaaata tttttttaat tcatttatta acattctgta tcgcttcctt
     6361 tctcaataga tagatttgat acaaacgaaa ttctccataa tctaattgtc catctacatt
     6421 ccattcgcta aagcgccact ctcgcacgga atccttattt ggttccaaac gttcgattgt
     6481 ttctgcagag agaatccact cgtatggaaa atcatcaaaa aacaaggccc actcaataag
     6541 atgatctgtt actgttcctc tttttaaatg acggattgcc atgatctggt caattgtctg
     6601 accattcata aacatctgct tggtgatcat catgctttga ttgaaatttt gctgtaataa
     6661 tgaatcgatc aaagcgtgcc aaagcgaatc tggccgtttt tcgatttgtg ctaacaaaag
     6721 gtggatacat ctgctttgtt gcagataaac ctctgactca tcattgtttt ttgccagctg
     6781 ataaggaagc agccctgttc gctcatttcc agataattgg ttagctaaaa aatctgctgc
     6841 ttcttctggt atctccgaaa acagctgcac caaactctca tacgcagtat caatcaaaat
     6901 ttcacgtggc aattggcttt cactaagcca tttttttagt tggaaagtat aaaaaggact
     6961 tgtctctgct ggaagatagt cttgaatacc cgttgccaga ttagaaatta cttgaacagc
     7021 aaatttaatg agccgccaac tagtttcccc tgttctgcca tagcggtcaa aacgcaaacc
     7081 agtatgttct atttgatgtt cggacaacat atcagcccct gcaggcgtta gctttgcttc
     7141 attttcattt atttcgatcc aaccttgctg cgcaattttt tgcaaaattt gataaaactt
     7201 atcctgcttc aattctggaa aactgttatg gataaacaaa agttcgtggg taaatccata
     7261 aataagtact gaagtcgttc gttttcccac aaggagttgg taaagagaac taatccgcag
     7321 cttgtgtcct gatgaaaata gcgataaaat aaaaacaatc atattattct ccttaagtta
     7381 ggatggttag aaatgtcact actatgtaaa cttgtacctg aacgctgtat tgcatgcggt
     7441 ctttgtcaaa tcgaagcgcc agatattttc gattatgatg aagaaggaat cgttttattt
     7501 gcccaagaac cagaggctca tcaacagttc gtgatgccgg aagaagaaac atatgtaaaa
     7561 aaagcgtatc aaaaatgtcc agttcgagcg attttattag aaaataaaat ataaatataa
     7621 acgaaacttt aaaaacgtac actaagttct ctaaacttga aaataagccg ggatctgttt
     7681 ctcttgttcc gtgaaatccc gacttatttt gaaagattca tttgctatct atagttaatt
     7741 gactagtttt ttcacggacc ctataggacc catttatatt atttcaactt tttcagccag
     7801 ttttccaatg tattgacgat cacagtttgc tggtctgcgt tagaaatttc tgcctctcca
     7861 tctccttttt gctcaccata gctgccaaaa cctccatgat ttccgccatt gattgataca
     7921 tactctgttc tttccggtaa atactgctta gcttcttgat aagcttcctg attcaatact
     7981 ttatcttttg tcgccgtgat cgataaaaca ggaacagctg attctgtcaa tgttcctttt
     8041 ttgtcgggat aacttgccat gaagaatacc ccttttaaat gtttgtctga atgattctta
     8101 ttgacataac gacttgccat tacgccacct aatgaatggc caccgatcac ataatcggaa
     8161 tttggaaaat cttctttgac cttatccgct ttgtttccgc ccatcactgc tagatcaagt
     8221 ggcattttga caatagcgac tgaaaagccg ttttttgcta aatcttgcgc ccaaatacta
     8281 taactttctg gctctacgag agcaccagga taaaaaataa gtaatggaga ctttccatga
     8341 ttctttttct ctccttcaaa aaataagtaa tcttctttgt tttttccttc ttttaccaac
     8401 acttgcgctt ctgacgatgg ttcgtacgta ttttgctgta tatagtaata accggctcca
     8461 ccgagtaatc ctaaaatgat gactaagcca attagtattc gtctaaatat tttcactttc
     8521 tcccaccttt tctatctgag tgaccccttt ttcttaatca agtatgatta taccttaaaa
     8581 aaataagaga gaaatgcaaa ctttccagca tcaaataaaa aaagacggaa tatccatttg
     8641 aatatccgcc ctttttgtat gtttattttt atttagcaca taaacgatac acagcttctg
     8701 cataaatttc cattgcttta tacatactac ttaattccca tcgttcattc gcctggtgca
     8761 taaagtcagg agtatcaggg aacatcgcac cgaatgccac acactggttc atcgtacgag
     8821 caaaagtagc gccgcctgat acaaacggtt cagtcatgtc tccagtaata tcgcgatatg
     8881 tccccaatag atttttcact aactcactgt ctagtggcac gtataatgaa tcaaggaagt
     8941 caaattcttc ataagtcaac ccatattttt ctgctgtttt agttaattta gctactaaat
     9001 catctttctt gaatgtaaca gggattctca tatctacacc gatttttgtt tgttctggtg
     9061 tgatttccaa acttgcaaag ttcatcgtta gctcgccgga ttgttcatct tctgttttgc
     9121 ccacgacagc ttcaccagtt gcattttctt gaactaattt tcctaagaaa ttgattgggc
     9181 caaaatcaaa tacttctgaa agagcaattg ccagacgaga aatagcattc actccttgtg
     9241 ctgcatcttt cgcgtggata cttttaccaa gaacgacgat gccttcacct tgttcttcga
     9301 aatcgaattc gtgttttttc aatgcttctt ttacttcaga aagcttttct ccagagtaag
     9361 gagctgcatc aggaacaaca ttcaacgctc ctccagcttt tacagaaaac tcgttcgttc
     9421 ctggtcctgt caagtaagct tgcaataagc ctttttctgc ataaattaaa ggaaattcag
     9481 catctggcgc aaatccttgc gtaattcctt cttctttttc gttatatttt tctaaacaac
     9541 gccataaatt ttcttcatct gtaccaaaga taaaacgaat acgtgtcttg aattccacgc
     9601 cagcatcaat caaagctttg acggcataca ttgccgcaat agaaggacct ttgtcatctt
     9661 gggatccgcg accaactaac cagccgtctt ttatagttgg atcgaatggt tttgtttccc
     9721 aattattttc gtcccctgct ggaacaacgt ccatatgaca caagataccg aataattcat
     9781 ctccagagcc aatctctgaa taaccgtaat agccttctgg atctttaaat gttctaaatc
     9841 ctagcttttc actgatttcc aatactttat ccaaagcttc aatcactttt ttaccgaagg
     9901 gatgtccttg tcctgaatct gcttcatcta gtacggaagg aattcggatc aattcgctca
     9961 atgattctaa tgcttgttca tgatgttgtt ctgtcacaaa ttttttcatg atattatcct
    10021 cctaatatgt taaaaagggt catgtcaaat tcctccgaca caacctcttc ttctcaaata
    10081 atgacgtttt aagcctgctc catcagcaag cagaatgttc ttaacaaatg atgaaagaaa
    10141 aaacactcac tgaatggagc tattgacttg tccacgtgct tatcgatagc cgtttatcgg
    10201 tttcttcatt cttatgatag accttgtacc tgtatttgtc taggtttaga acattgctgc
    10261 tacacctagg atcacacaga tggctacaaa taatatcgca attaatttac cggtaaattt
    10321 ccaccacact gaaacatcga aacgagcaat tgctacagca cccattacga tagcagatgt
    10381 tggtgtaatt aagtttacta aaccagatgc agattgataa gcagtgatta ctaaatcctt
    10441 accgacgccg gcaaattcac ccataggtcc catgattccc attgttgcag cagctaagcc
    10501 ggaagttgaa ggaatcaaga atgacatagg gatatagaaa ataaatgtaa taatgatgaa
    10561 tacacttgaa gataatgaac ttaatccttg ttcgccccaa tgtaagatgg tgtctgtgat
    10621 taatccattg ttcataacca cttggatacc acgagcaaca gctactacga ttgctacacc
    10681 gatcaaatca gaagcaccag ctaaaaattc agagacaaat tcagactctt tcatgccata
    10741 agtgattgct actacgatgg acattactaa gaataacata gtaatttctg ggaagtacca
    10801 gtcacctaat ggaagcatgc ttttacctag aaccgtacct agtaccggaa ggtttgttaa
    10861 ccagtttgtc aatgattcaa aaatcgtgaa attcgcattc aaggttgtcc aaggaatcaa
    10921 actgatgatc atgataccaa aagtaataaa gaacaataca ttaacatgtt tttgtttttt
    10981 cgtgattact tcttgacgac cgattgcttc aatatcgaaa tgcttcatat cttctttacg
    11041 ttggttgtat actaatgatt ttgttggatc tttttcgatc tttgatgcat aacgatacac
    11101 atatacaata gaaatagcta acaaaataac gaacatcaag aatcgaagac caataccttc
    11161 acccatcgaa atcccaacag cttgtgaagc tacgcctgtt gcaaaaggat ttaccgttga
    11221 agctagacag cctacttgag accctactaa tacgattgct acagctacta ctgtatcaaa
    11281 gcctacagcc atcatgactg ggatcagtaa agcatagaat ggaatcgttt cttctgccat
    11341 tccgtaagta gagccgccta atgcaaataa cagcatcaat actggaataa gcattttttc
    11401 ttttcctttg aatttcttca caactgaagc aatcccggca tctagtgccc ctgttttgtt
    11461 gataatacct aaaaatccac caacaaccaa aatgaagaaa gagattggga ttgcagcagg
    11521 tgtttcttta gttagctccc ccgctggtac acctagcatc ccattgattg gcgccatgaa
    11581 aatatcccac agcccttgag gattttgttg tactcgttca tatgatccag cgacgatgtt
    11641 gcctgcatcg tctacactat agtgcccagc tggtataaac caagtaagta cagcgattac
    11701 tgcaataata ataaataaga ccgtatacgc tgaaggcatt tgaaattttt tcttttttgt
    11761 ttgttccatc ttcttcttcc tttcttttcc gttagagaca acaattttgg ctctaatgtt
    11821 attttatcaa aaagagtatc agaacaaaaa taataacacg cattttgtaa aacttttgtt
    11881 caaaaagttc gtttttccct attccctcct cttttttcag gtttttaaag ctttcttctt
    11941 tttcattgta gcgtttcctt ttttttataa acaaacattt tcgtctttta aaaggagaag
    12001 aatttcacat ttgcaaagaa ttgattgatt tttatgcata aatcaaaaaa atactcaaaa
    12061 caaacaaata aaacgtttac attattgcat gtcttgtatt tttttccaaa aaaaaagacc
    12121 aagaaatttc ttgatctttt tgatatttat ttgactgctg tatgacgttg ttcgaactga
    12181 tgttgttttc gacttaacca tggaagaagt tgcgcatgca gcactaaaaa gacagcactc
    12241 acgatgaatc ctttgatcaa gttgaaagga acgatcccaa tcagtacata gtttgctaaa
    12301 gacatgccca gcatctgtgt tgcgttcagc cctaaaaaca ttaagtaaag aggcgtaata
    12361 acgaaataat ttgcgatact cataaaaata gttagagcga tggttcctgt aatgacacct
    12421 aaaaatttgt ttcttccttt atgctgtttc tttacactaa agaaataata aatcgggaat
    12481 gtaaagatcg ttgttgataa gaaactagca gtgtctccta ccaaattatc cggagaaaaa
    12541 cctgtagtca gcagatgtaa tactgaacgc aagaacgctg ttgcaactcc ggccgctgga
    12601 ccgaataaga acatgctgat caataccgag atatcactaa aatcgatctt caaaaaacta
    12661 aaagccggca tgatcggaaa agcaaaatac tgtaaaacta atgcgatcgc agcaaacatc
    12721 gctactgcaa ccattttctg tacacgggtg ttcttcattt gaaatcctcc tgttaggact
    12781 cctcttcagc gaactttttg acgtttctct ccaaatgaaa aaactctatt ttccagggaa
    12841 aatagagtta gagttatttg atcaagcgtc gtcaaataag ctctatcttc tttatccaga
    12901 ctatactgtc ggtatcggaa tttcaccgat tcagctattt ttttgcaaaa taggtcgtgg
    12961 actataacca ccggtcggga atttcaccct gcccctgaag acgaacctta tataattttt
    13021 gtttacatat tcattataca tatctattca aaaaacgcaa catctaacac ttataaaaag
    13081 taagtgttca ttttgagtgg ttcagcaaac tgctgacttc ttttttgttc agttcacggt
    13141 aatccccagg acgtaggcct tgtaaagtca gatctccgta tttttcacgt tttagttttt
    13201 gcacaggaag accaactgct tgaagcatat ttttcacttg atgattacgt ccttcgtgga
    13261 tcgtcaattc cacgatactg tggtttgtct taggatccac tgataaaatt tggaatttag
    13321 ctggagatgt ttttcgtcct tcgatcttta ttccttttgt caaaggtgca agcatcgatt
    13381 ttgtagcgat cccttttacc tttgcaacgt aaactttatc cacttcatgt tttggatgtg
    13441 tcaggcgctg agcaaaatct ccatcatttg tcagtagcaa gatccccgaa gtatcgtaat
    13501 ccagtcgacc tacaggatag atacgttctt ttaccatcgg caaaagatcc gtaacaactt
    13561 ttcttccttt gtcatctgca acagcagaaa taactccttt aggtttgtac aataaatagt
    13621 aaccgtattc ttcttgataa attgggactt catccacttc cacagtgtca tgtttcccaa
    13681 cttttatccc taattccctt acaacttgtc cgtttacttt tacacgtcct gcaaggatga
    13741 attcttctgc ttttcgacga gaagtgatgc cagcgtgggc aattactttc tgcaatctct
    13801 ccattacaat tattctcctt tattcattcc atcaaagaat agatctggtg acacgtcttc
    13861 ttcaagtgaa atttccattt cttgtgtatc aggcagttct tctaatgatt tcaaaccaaa
    13921 ataatctaag aagtagccag ttgttccata taatatcggc cgacctggtc cttctactct
    13981 acctttttct tcaatcagtt tatgggcagc tagacgctgt accgctcctg aaacctgaac
    14041 tcctcgaatc tgctcgattt cagctcttgt gattggctgt ttataagcga tgattgctaa
    14101 ggtttcgacg gcggcttgag ataatgtgtt tgatgtccct tgcgcatatc gcttaagtaa
    14161 gggagccagc tcttttttag tcgttaatac ataatggctt tccgtttcaa aaatatgcaa
    14221 agctgagtgc gagttttgag tgtaagattc ttttaattct ttcactaaac ttgtgacttc
    14281 tgcttcgctg agatttaaca gtccgcttaa ttcttcatta ctgatccctt cctcgccaac
    14341 aataaaaaga agggcttcaa gttcacttaa tttcgtcatg ttcggtatct cctactgctg
    14401 gataaagcat aattgattca tagttttctt tttgttgcgc aatgatctgc ccttttttca
    14461 tcaattctag caaagccata aaagtagtga caacttcact tttagaaggt gactcaaaaa
    14521 aagaatcgaa agtacagcca tgcgcatttc tttttatacg ctcttcgata aataccattc
    14581 gttcttcgat cgttgtgtca tcgggcgtga tcgttttttc cactttttga gaaaatttct
    14641 tacgttctaa gattttctgc atggcacgga tcaaatcgat ttttttgact tgtcctcgct
    14701 tcaacggctt ttcagcatcc gctaacccat cgatgttcat aggttctttt gtgtaataaa
    14761 gacttcgttc ctctgctttt tctgtcagtc tttcagctgc atatttatat ttacggtatt
    14821 ctaaaagctg agtgaccaat tcttctcgag gatcttcttc ccataattct tcatcgtctg
    14881 tgccgaattc ttggacagga agcaatgtct tgcttttgat tgccatcaat gtagcagcca
    14941 tcactaagta ttctcctgca acagctagct cgagtgtttt cattgtatgg atataattca
    15001 tatattgctc cgtgatcgta gcgatcggta tatcatatat atcgatttcc atttgctgaa
    15061 tcagatgaag cagcagatcg agaggaccct cgaatacatc taatttaata ttgatctccg
    15121 tcaactcatt cactctttct tctttttctt tgctagccat tttccaatgt aaggctctgt
    15181 gatttcagtt gcacggatgg agacatccgg gtacttttcc tgtaattcat ctaacatttg
    15241 aaagcccata ttctcaccgc gataagacgg attcaaagaa atgtcatgga tgatcagctg
    15301 atcgttcgtt gcccagtaaa taccaataat cccttgaatg ttggttgaac cttctggcgt
    15361 aaagaaaaaa agttcataat cgtcttcaga ttcgtacgta tcaatctcct tcaacaaaga
    15421 agttttacct gtcagtttct tatggaaact caatagtccc atagcaattt tacggttttc
    15481 tttccggtaa tgtgttaaca tcttctcacc ttcctatgac taagcccttg gaaaatgctg
    15541 cttataaaca tccgtcattc ttttcttcgt gatatgtgta taaatctgag tcgtagagat
    15601 atccgcgtga cctaacaatt cctgtactgt ccgcaaatca gctccatttt ctagcagatg
    15661 tgtggcaaag ctatgccgca gcgtatgtgg tgttacttct ttgtaaatcc cagcctctcg
    15721 tactaactgc ttcagatttt tccagattcc ttgccgagaa agacctttcc catgattatt
    15781 gacaaataca tgcatttcag atgcgtcctt tacaagtaat ggacgtgctt cgtctaagta
    15841 gcgttccagc cattggatcg cataatcacc taaaggaatg atccgctctt tatctccttt
    15901 tccaactgtc tgtaccagtc caagagaaag atgaagatct cccaatttca atccaatcaa
    15961 ttcactgacg cgcatcccag tagcatacat gacttccaag attgcacgat ctcttatccc
    16021 taacgttttt gtcgtatcag gtgcttcaat cagcttttct acttctgtca gagtcaatgt
    16081 acttggtaat ttctgtgctt tcttcggcgt atcgatatgt tgcatcggat catgatcagt
    16141 gatacgctct tgccgtaaaa actgatggaa tcttctgaga cttgagatca tccgagtaac
    16201 agttgctgtc gctttttttt cttcatgcaa cttctccaga aaacggatga ctaaaaagcg
    16261 atcgacatcc tgccatgaag caattttctg ctcctgtaga aaatgcaagt actgttccaa
    16321 atcacgttca tagctttgtc gtgtattggc ggacaatccc cgttcaatat tcaagtagtg
    16381 gagataatcg ttgatttgct ctttcataat aatccccctc atgcctctct atcatatcag
    16441 ataccgcttc attctgatag tctttttcca taatctgagc aaagaaaaag ccgtctagac
    16501 actcagacgg actggtttct ttt
//
LOCUS       DAFBQH010000061        16348 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.063, whole genome shotgun sequence.
ACCESSION   DAFBQH010000061 DAFBQH010000000
VERSION     DAFBQH010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.063"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="LK694_002035"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="LK694_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HBK5052881.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2944..3378)
                     /locus_tag="LK694_002036"
     CDS             complement(2944..3378)
                     /locus_tag="LK694_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052882.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3395..4600)
                     /locus_tag="LK694_002037"
     CDS             complement(3395..4600)
                     /locus_tag="LK694_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HBK5052883.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5294..5680
                     /locus_tag="LK694_002038"
     CDS             5294..5680
                     /locus_tag="LK694_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBK5052884.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5774..5965
                     /locus_tag="LK694_002039"
     CDS             5774..5965
                     /locus_tag="LK694_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5052885.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6479..6811
                     /locus_tag="LK694_002040"
     CDS             6479..6811
                     /locus_tag="LK694_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5052886.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            6721..6915
                     /locus_tag="LK694_002041"
     CDS             6721..6915
                     /locus_tag="LK694_002041"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK5052887.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            7107..7262
                     /locus_tag="LK694_002042"
     CDS             7107..7262
                     /locus_tag="LK694_002042"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBK5052888.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7538..8878
                     /locus_tag="LK694_002043"
                     /pseudo
     CDS             7538..8878
                     /locus_tag="LK694_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9119..10735)
                     /locus_tag="LK694_002044"
     CDS             complement(9119..10735)
                     /locus_tag="LK694_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBK5052889.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            10853..11071
                     /locus_tag="LK694_002045"
     CDS             10853..11071
                     /locus_tag="LK694_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5052890.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            11266..11727
                     /locus_tag="LK694_002046"
     CDS             11266..11727
                     /locus_tag="LK694_002046"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK5052891.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(11920..12159)
                     /locus_tag="LK694_002047"
     CDS             complement(11920..12159)
                     /locus_tag="LK694_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5052892.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(12183..13706)
                     /locus_tag="LK694_002048"
     CDS             complement(12183..13706)
                     /locus_tag="LK694_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBK5052893.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(13724..13846)
                     /locus_tag="LK694_002049"
     CDS             complement(13724..13846)
                     /locus_tag="LK694_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBK5052894.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(13876..14859)
                     /locus_tag="LK694_002050"
     CDS             complement(13876..14859)
                     /locus_tag="LK694_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBK5052895.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(14883..15032)
                     /gene="rpmG"
                     /locus_tag="LK694_002051"
     CDS             complement(14883..15032)
                     /gene="rpmG"
                     /locus_tag="LK694_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBK5052896.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(15053..15430)
                     /locus_tag="LK694_002052"
     CDS             complement(15053..15430)
                     /locus_tag="LK694_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052897.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(15462..15641)
                     /gene="rpmF"
                     /locus_tag="LK694_002053"
     CDS             complement(15462..15641)
                     /gene="rpmF"
                     /locus_tag="LK694_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBK5052898.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(15656..15925)
                     /gene="rpsN"
                     /locus_tag="LK694_002054"
     CDS             complement(15656..15925)
                     /gene="rpsN"
                     /locus_tag="LK694_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBK5052899.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
BASE COUNT         5184 a         3096 c         2455 g         5613 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtcctt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2521 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2581 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2641 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2701 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2761 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2821 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2881 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     2941 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3001 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3061 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3121 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3181 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3241 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3301 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3361 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3421 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3481 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3541 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3601 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3661 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3721 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3781 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3841 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3901 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     3961 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4021 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4081 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4141 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4201 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4261 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4321 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4381 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
     4441 taatattatc agttagtaac tttttataat cataaaaaaa ataagtagtt ctatctgagc
     4501 gatactcttt tataatactg aattcggaat taaaaaccac gataggtaac tttaaacaaa
     4561 catgaagaga ctttaataag acttctttat ctgaaaacac ttttgtttct ctctttcctt
     4621 tctacatcat taaatagtat tttatacgtt atctaaaata ctattcctct gtcaaaataa
     4681 gctaacttac ttcattataa aatatccact tatcgctttc atgtatattc caccgtaaaa
     4741 agtacatttt atcaccgaga actcatcatt tttcatcatg atgaatacta tttacatata
     4801 tctagttcaa agacaataat tattatgatc cgttattatc taagcactat acagataata
     4861 ggaatttttc gtttctaaat attccaattt gggaatccaa taaaaatttt cgttcaaata
     4921 cgtgtctatt cgaactcttt tctagttgtt aattatgaag ggggaagaaa taaaaaatac
     4981 aatctataca cgaataagaa tattatccaa aaggaggttt tagaaaagat ttattatacg
     5041 taaaaagctc aagacatttt cttagccata aggacatgtt tgatatttaa gaagatatat
     5101 gaattttctt ttctaagtat aaagataact atccattatc aactaaaaaa acatttttga
     5161 aaataaacat cagcagtcct atcaatgcat gtgagcttat agaagttatt cgcagcgcag
     5221 cataattgca ttagcttctt ttcgtgtttt ttgcctcttt tccctttaaa tcttaatgga
     5281 ggaggtgtgc aatatgtcag ataacgacga acgcgtaaat gcccagttga ttcgattctt
     5341 tgaaaaggtc attttgaaca ccgctaatac ttattataaa aaacaagaag aaattcaaaa
     5401 acatgaacaa cttgatcaac tcccaccata tttgtttact aaagatacta taaaatttaa
     5461 acaaccgatt aatgttttaa atgtgactct ccttgttgaa gatataaaat tagcaaaaat
     5521 ccttcctttg ttgaaagaac ctgaacaaat ttttcttatc gaaaaattta tctttgataa
     5581 aactgataaa gaaattggaa aggttctggg gattacaaga cagggtgcga caaatttaaa
     5641 acatcgactt tacaaaaaaa ttcaaaaaat gctggtttaa tatctgcata ataactaatt
     5701 ttgtatgctt ctttgctcgt tctcctcctt cccaaagaaa tagattataa gtccatggga
     5761 agaaggtaga tttatgaatt ctttaaaaga agatttcatt ttagcaaagg ctggaaatga
     5821 agaagctgtt gaggcaattc tcaaaagatt tagctctttg attcataagc aatcctggag
     5881 aactggaaaa tatgatcaag attgttatca agaatgtatg cttgcaatat atctagctat
     5941 ctcgaaattc gaaattaaag aataactcaa ctaagggagt ctgtgacata agtaaaaaga
     6001 tagtttcaaa tttcgaacaa taaatttgaa actatcttct ttataattat aaagtatcga
     6061 ttttacagcg ttctataatg attcatagaa tctaaaacga atgattttaa ttatcaacgt
     6121 tttttaattc gtttttagct taaaaataac ttttgtcaca gtctcactcc taaaattact
     6181 ttatttacgt atgaagtggg caggaggtcg aatccagaac tgggctatgg tacttcaacg
     6241 aggacttctc ctctagaatt gaaaagcatg cgatttacct tccgtaacga cggtagattt
     6301 attcaactat gtgtccaatc aaaatttgtc tgatttcaaa aatagtcaag agaattttgg
     6361 ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt gacaaaccct gatactaagt
     6421 tgggctatga tacacttttc ttgactatca gttacgttac cggaaggaat gaactctatt
     6481 gagaaaaaca tatgatcgta aatttaaact aaagatttca caggatatcc ttgagaaaaa
     6541 agtaactacc aagaaaattg ctgaagaata caatatctct cgtccaacaa tttcccgatg
     6601 ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct ggacaaggaa aaagattacc
     6661 tgataaagca gatttctaca ttcttgaaca ggaaaataaa cgactaaaag aggaagacga
     6721 gtgttctacg aacacaaaga tcgatacgga tctccaggga tcactcaaaa actttacgat
     6781 gaaggaatcg aaatcaataa acgtgttgta gctgttctta tgcgtgaaat gaatttatgc
     6841 acaagaggat ttcatcgacg caaatcttct catggtagag aaaaaacaat caaagaaatg
     6901 gtaaaaaaat gttgaatcga taatttgaac agaaccaaat tgatgctgtt tgggtgatgg
     6961 atattactta cattccatgt aatgacggtc gcctctattt gtccacctat attgacttag
     7021 caactcgtat tctacgttac tatatggtag attcacacat gaaaaaagag attgtcgtcc
     7081 gtccattaca aatttattaa ggagaattgc ctacggtaat tcattccgat agaggtagcc
     7141 agcatcggtc ttttatttat caagagctgt tgaacgacca ccacatcaca cattcagtga
     7201 gccaaactgg gactcctgtt gacaatgcag taattgaagc gtttcatcga tcaatcaaat
     7261 gagaattaat cgactccaac aaatataaaa gtaaaataga gatgaaggtg ttaatccaag
     7321 attatttaga agactactac ccaaacaaaa gaatccatac aaaatttatg atgactcctt
     7381 gacaatttga agcgaagaaa ataagggatc tataatatct agtgttggtg acgccttctg
     7441 ttgccaatac tttttctttt ttctaataaa cgcaaacaga caccttaaaa tcctttagaa
     7501 taaagtcgac gaaaaccatt caaaggagtg tttcaagatg tcctattcag agtttaccaa
     7561 agagttacta gatattctag acctaaatct tatctttcat gaagatgcct ttcgcaaaga
     7621 gcgaatcaat gacgaaacat gttttgtttt tgatggcaca ttgacctatc aaccggagga
     7681 atgctttcat cgccactatc aaaatgaaca aacgatcatt aaatagggct ggaaaaaagt
     7741 atccatctta ttgaatgatg tcagtaacta caagaccatt ctgcggatta acaaacagcg
     7801 cttcaaatgt aaacactgtg gaaagacttt tttagcagaa gattcagtta gtgatcgtcg
     7861 ctgctccatt gctcgcagag tcaaacaagc cattcttgag ctattaagcg aacctctttc
     7921 tatgtctttg atcgcacgta tgaagcacat ttctccgacc actgtcattc gaatacttcg
     7981 cagccttcgg cctaaaacca tttctttgag ccagctgtta ccagaagtgg tgtgtttcga
     8041 tgaattcaaa tcagtgaaaa atgtctcggg agccatgagc tttgtcatga tggacggcaa
     8101 gacccatcaa ctgattgata ttgtggaaaa tcggcaactc aatcctttac gcgactattt
     8161 cttgcgctac cctataaaag tccgtgaaca agtgcgtcta gtggtttcag atttctatac
     8221 accctatcgc acattggtga aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt
     8281 tcatattagc caacacattg gtcgggcttt taccaaccac cgaattcaag taatgaagac
     8341 ctttaaaaaa ggggatcgtc ggtcgaagca cttaaagaaa tactggaaac tacttcaaaa
     8401 gaatgcgtgg gaattgaaag gtcagcatcg ctactggcgt ccgtcatttc gagatcatct
     8461 gacagaagca gagatagtcg atcggctatt atcttatgat gattccttaa agcgaggcta
     8521 cgaagtctac caggattttc tttccgttat cagaagacaa gatgttcctg aattcgtcgc
     8581 gctcctaaaa gaagactaca aggagttgcc agagcactac caaccagtgt tcacgacctt
     8641 caagaaatac caaacagaaa tcaaacgagc gttgcgcgtt ccctattcca acggccctat
     8701 agaatgtttg aataaccata tcaaagtact aaaacgaatt gcctatggct atagaaactt
     8761 tcaaaactac cgagaacgaa tctttttata tcgaggaaaa tattttaaga aaactaagaa
     8821 tactacccaa ctgactaaag ccagaacaac aacacggcta gacaagatag ctgtctagcc
     8881 gtgtgagtat ctcgattatt ctattgaatt tcttcaccaa cactcattga cgaagagcca
     8941 aaataagtaa tctaaccgac tgttaactaa accattgcag gtcccgttaa agatacttgt
     9001 gcctgttgtc accgcttctt tttcttgtcc acaagcgctt aacacactta ataaagcccc
     9061 tgctattatt aataataata tctttttctt attaaaaacc tcctcatcta tttccagatt
     9121 agtaaatgta gtaatctgtg ctttcttttt tctcaatact aataactaac ttatggggta
     9181 aacaaatact cgtttgaccg ggttttgaga tccatccagt tctaaccgcg atttgatcag
     9241 gactattatc ttctttaact cgaattcttc catctttaat ctcaacaata ttatattgtc
     9301 ccttcgcagg atgataattc acgagtttat gattgatctt atccaaatcg aagcgatcca
     9361 tttccttgcc atcaattcga acgatggcat atttgatagc atcttcagtt gtttcattct
     9421 ccttttctat agagaataca aataagggaa taaaagaggt aataatcaaa atgccaataa
     9481 ttatgacatc gcctatcttg acaccagctt ttcttaaaaa tttcattaac gatatctggc
     9541 taaacatatt agactttttc catggaaatg gtgaaatttt tcggaaatta tctgcattaa
     9601 gagaatcagt taaacaaccc tcaagttgtt ccttaataca ttgttgatcc atcttcatac
     9661 caataaaaac tagtttatta atgcgttcac caaacaacgg atgccaggct tccttcaatt
     9721 cgggattttt ttgtataatc atttgacact caattttagg taaactagct acccaataat
     9781 ttaatgattc aatactttta tagctacccg atgtttcaaa gatcgtcgaa aaatcactat
     9841 gttgggcaaa ccaaagaaac ccttttgctc ttgtaatatt gccaggaaaa tttttatccg
     9901 taagatgggc aaatttttgt gcgtcaagga gtaaccgatt tcgatagata aaacttgaga
     9961 taccatattc ttctgtttca ggaatatgat tttgatagcc aagctctaac tctttgaacc
    10021 agcccactga ataggaagtc tcatctatat taaaaagatt tgtatttaga attttcgata
    10081 agtctacttc acctttaatt gtcctaatta ttttggcatt ggactgtaac ttagcaatta
    10141 gaccgttaat tttgtaaatc gtcttggttg acactaagtc actattattt aaaagtaata
    10201 tgttacaaca ctcgaattga tcaattaaca agccacgaat atcctgttct tcttcaacta
    10261 cattaccttc agcttttctt tcctgaatct tttcacccgt ttcgaaatta tcccaaaaac
    10321 gttttacatc cactactata ataatggcac caatctctaa tcgttgggtc aaatcaatgt
    10381 ctaaagggtt tttagctaat ataattgctt gaataattgg aattggtttg ctgattcctg
    10441 aagcttcaat aacaatttga ttcaagtcag aaaattgcac taattgatct agttcaacaa
    10501 tcaaatcttc gcgtaatgta caacaaatgc atccattcgg catcgaaaca agtttttctc
    10561 cagttctttt aaaacctttc ttgtcgacta ataccccatc aacatttact tcccctatgt
    10621 catttacaat gacagcaatt tcctttccgt gatcaccgta cagaatatga tttagaagta
    10681 tcgtttttcc tgaaccaaga tagccagtta agattgtaat aggtattttt ttcataggga
    10741 acctctttct ataccttgaa tttacacttt aagtgcaaca cataaaaatg agtcataatc
    10801 gatatccaac tagtataagt ttgtcgagaa cactcctaga aggaaaataa ttatgagcca
    10861 gtatcgtcat cttactatac tcgaacgaga aagaatcttt ctgcttcacg aagaaggatt
    10921 ttctattagt ataattggca acgacattca gcatagcccg tttactattt tacatgaact
    10981 gagaagaaac aagaaaaaaa ctattcacct tccaaagcgg aaaaaaatta tgcttatcga
    11041 agaaaaaaat gcggacgtta tcacattcta aatgatccgt gagtttggtc aattgttcgt
    11101 catttatttg tcgattttca gtggtcgcca gaggaaatct cgaatcgttt gaagcatgaa
    11161 catatggact tttcaattag ttttatcacg atctaacgtg caatttgcaa tgatcttttc
    11221 gatacagaac ccctctcaca cggcaataaa ggcctgatcc gttcattgca ccatcgagga
    11281 aaaacaagac atacaaaaaa tcatgtcgag tctcgtggaa agatttgcct tactcattcg
    11341 atccatggcc gtccaaaatc cgcagatgat agaagtgatt ttggtcattg gaaagccgat
    11401 attgtagctg gcaaaactgg tggtacttgt ttagtgactt tgacagacag aaaatcatgc
    11461 ttccttttag caggaaagaa ggtccccaga aagaaagccg cttatgtgag cgagaaaatg
    11521 attgaacttt taagcagtat tccaaatgag gcggtcaaaa cgatcactcc agatagagga
    11581 aaagaattct ccaatcattt cgcagttaca gaagcattga ataatatgcc cttttatttt
    11641 ccagatcctc atgcccttgg caacaatgaa caaattaaaa tacgaataga ctcattcgag
    11701 aatacttgcc aaaatcgaaa gagatagcta cggtagataa ctcaacgata aatcagatga
    11761 ttgaaaaact gaataacaga cctagaaagt gtttagattg gagaacacct tatgaagtct
    11821 tctttgataa agtgttgcgc ttgatttgac aattcaagac ataataaaaa gcagatcaaa
    11881 tccaaataag ggaaaaatga tctactctgg tattatcaat taattggcca agctattatg
    11941 ccgttttgct aaacgtctac ttcttccagt attgaccttg ataaaacaac aatcaggatc
    12001 ccgttttaat actctatgaa ctgtgtcaat actaacatgt aaagttgcag caatatcagc
    12061 taatgtactc ccatttaatc tcatttgctt tgcaaatcga taacgccgat aagcatcaat
    12121 attcttggaa tccatagaca ataatacttt tcttaccatg tatttcctcc aaaaattgaa
    12181 ttttaatgag ccatcatttc ttgagcaatt tcatgtttag acaatgaagc tggatagaaa
    12241 gtaggccaat tatgcaattc attatagagt ttttcttgac tttctccacc aaagaaaata
    12301 tggaaatgct ctgccttact tggtgcaatt ccatgatcac taaattgaat atatttaaaa
    12361 cgaccagcac ctgcatcatc agtttcaaaa ttaaaacgaa caccacggtt tcctttttca
    12421 taattcaaaa ttttgtatcc aacatatttg tatgtgtatt gatgatgttc accgttgact
    12481 acaaaatcaa tggtgttgtc tttaatgttg atagtatcaa tatctgtctt atagccagta
    12541 gtataataat ctttgtactc ggcagctgtc atgtctttat tgatttttgc tttgtaatcg
    12601 aaaacttcat ccaaaattcc atcttttaac aacggataaa cagattgcca ttcgcctgta
    12661 taatcactca attcgcgatc cttcacatcg gcatctttaa agtacccatt agcaactgtc
    12721 ttttcatcct tcgcttcttt ttctggaact acctcttcac cagcaaccat agttgttttt
    12781 tctaacgctt ttagattgtc ttccattact gaaacataat cttcaccatt atccatttgt
    12841 tcttttgtaa gactttcaag aggattcagt acttccaatt ttatcccagc ttcgttggcc
    12901 aaagtttttg caattttatc attagcattc ttttcaaagt aaatataatt gatcttattt
    12961 tttttaacat actcttttaa ttcagctaat cgaccagaag atggctcttc ttcaggactt
    13021 agtccagcaa taggaacttg tttcaatcca taatccaaag ctaaatatcc aaaggctgcg
    13081 tgttgcgtca caaaactttt ttgtttcgct tctttaagac tagtggtata gtcagcatcc
    13141 aaccgtttca gtttagtcaa atacttttca gcatttgtct caaatacttt tgcttttttc
    13201 ggatacaatt taactaactg atccttgata tttgaaactt ccttaattgc cattttgggt
    13261 gaaacccacg tatgaggatc tagttcatga tggtgatctt cttctccgtg atcatgatct
    13321 tcttcttcac tacctggcaa taaaatcatg tcttttgttc cttctactac attaggccca
    13381 gctttcttcc aactttcttt agcttttggt acccatgatt ccatgttttc gttatgatat
    13441 acaaaaacat cagcatcatg agcttttgcc atatccttag ctgagggctc atagtcatga
    13501 ggttctgatc ccgctggaac cattaaatcc acgttcgctt catcgccgac gatgtttttg
    13561 gtgaaatcat acattggata gaatgttgtt acaatttcta attttccatc tttatcattc
    13621 gatacttttg catcattagt gctggagcaa gctcccaata atcctagagc tgctatccca
    13681 ataagtccta cactgatttt cttcattctt tttccttcct taattatgct aatttcttca
    13741 ttttatttcg tttatgttct ttgataattt ttaatgccct ttttctagtt ttaatattcg
    13801 ggcttttaag tcgtcgatat gcactagata tatccgtttt ctccattaga aattaccccc
    13861 aatcactact tatcattatt tgctttccgc cagtaattta tcaaaactct gtttgatcct
    13921 ttcttcatcc aacatttttc cgattagaat aatagtggtt tggtcttttt gtcgagtggt
    13981 gtattgaaaa ttaacctgtt cgtttacctc ttgcatggca actataaaat cctgctcttg
    14041 taaagcaatc aatcctttaa aacgatacag gctcccttgg ttggttaaaa tcagccagtc
    14101 aatccattgg agtaaaaaat ctttttttat gtcttgatct gaagttaaga gaattgattt
    14161 aaactcatgt acttgatggt gttgatgata gtatttgcca ttatagtgat tttctgatga
    14221 atcactttca aaaactaagg gatgattaaa ttttttcaaa ttataaaaat cctttttttg
    14281 aatcgtttca ctatgtgtga acattcgaat atcggataaa gggttaatac ttttgatttc
    14341 tctctgtaat ttttttagtg cagataaatt caagttttgt tttctagaca caaaaatgcg
    14401 atctgccatg acaagttgct taagggcttg tggttctctt tcaataagtt caaaactgtc
    14461 actatcaata acagtcaata aactatcgat atatactctt cctcttagtt gaggcgctgt
    14521 taaaagtgtt tgaatgattg gttggggatc tgctatacct gtagtttcaa tgatgatttg
    14581 tgaaattggt tgttgatgtt cttctattgt ttccagtaaa gaaatcaata ttgctaataa
    14641 atcactgcga agactgcaac acatacatcc attatttagt tgtaacaccc gttcctcgct
    14701 atgaactaat aatttgtgat caatcccaac ttcaccaaac tcattttcaa ttataataat
    14761 ttcttccctg gggtagggtg agacttgcaa ggcttgattg attagcgttg tttttccagc
    14821 ccctaaaaac ccagaaacga ttgtaactgg tatgcccatt ttgtcacctt tttaccactt
    14881 agctatttca cttctttaaa aattgcccgt ctgcgcaatt ttggcgaata cttttttaat
    14941 tctaaccgtt cagggttatt ccgtttattt ttgctggtga gatacagacg ttctccagtt
    15001 tcgacacatt ccaaaataat attttgacgc ataaatcttc ctccttcaat acttatcgat
    15061 cttcaagata tgcttgaatt tgttgtgcat cttcaaaagt taactcgcca gattccaatt
    15121 gtgaaactaa attttctttc atatcaacag gtatctccca ataaccatcg atacattgaa
    15181 ataactcctc gtcatactct tcaaaaattg gatagattct taaaaaatca tcaaatgata
    15241 agccttggtc ctcatagtaa tctttgacta atccattttc aattgcctgt tgggtcggta
    15301 aacccgtttc agtaataatt cctctctcta ccatcgaacg ataaaaagtg catttatcta
    15361 gcgattgctg ggtcgtttca agataatact ttaatccttc ttcaactaaa ggtgggtaaa
    15421 taatttccat tttgattccc tcctaaaagt tatatattaa actattttga agctccttta
    15481 attactttct ttccattata gtaccctttt aaagatacat gatggctacg tctataatca
    15541 ccattcgctt cgtcaaatga aatttctggt tttactacag tttgatgagt tcttcttaac
    15601 cgtttcttcg ccttagatgt ttttcttgct ggtactgcca tgatatttcc tccttttacc
    15661 aacttgcttt ttttactcca ggaatcaacc cttgatgggc aagttctcta aacttaatac
    15721 gggacatacc gaactttcgc atataccctc ttggacgacc atccgtctga tcacgaagcc
    15781 tcagacgatt cggatttgaa tctcttggca atttagcgag agcggtgcga tctcctgcgg
    15841 cttttaattc ctgacgttta tctgcatact tttcaatcat cgccatttgt ttctttgctt
    15901 tagcaatttt cgactttttt gccatatacc tttatactcc ttttattttg atttagacac
    15961 ctcaaatcgt aatcgttacg ttttgacact gatactttac caacaagagt tgctcttgtc
    16021 aacaataaat aacatgaaat aaaactcata tgaatagata actatttgaa aacgaattta
    16081 tcggtacata gagataatca aatcatttaa aagaatatgc agtttaggct ctgaaaaata
    16141 agaattcact tttattagtc caatcaaaca caaataccgt tttaagtagc ttttcctcta
    16201 cttaaaacgg caatatgaaa gaaactataa ctttttattc aatcttttat gtttaactgg
    16261 tttatcgcca attttttaaa actttgatta ttccttatat tataagaata gacattaagt
    16321 gaactaattc aatcatactg attctttc
//
LOCUS       DAFBQH010000062        16108 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.064, whole genome shotgun sequence.
ACCESSION   DAFBQH010000062 DAFBQH010000000
VERSION     DAFBQH010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.064"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(862..1203)
                     /locus_tag="LK694_002055"
     CDS             complement(862..1203)
                     /locus_tag="LK694_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBK5052900.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1203..1433)
                     /locus_tag="LK694_002056"
     CDS             complement(1203..1433)
                     /locus_tag="LK694_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK5052901.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1720..2058
                     /locus_tag="LK694_002057"
     CDS             1720..2058
                     /locus_tag="LK694_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052902.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2263..2502)
                     /locus_tag="LK694_002058"
     CDS             complement(2263..2502)
                     /locus_tag="LK694_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBK5052903.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2478..2825)
                     /locus_tag="LK694_002059"
     CDS             complement(2478..2825)
                     /locus_tag="LK694_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBK5052904.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(3557..4138)
                     /locus_tag="LK694_002060"
     CDS             complement(3557..4138)
                     /locus_tag="LK694_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK5052905.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4321..4947)
                     /locus_tag="LK694_002061"
     CDS             complement(4321..4947)
                     /locus_tag="LK694_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK5052906.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4969..6300)
                     /locus_tag="LK694_002062"
     CDS             complement(4969..6300)
                     /locus_tag="LK694_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBK5052907.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6455..6856)
                     /locus_tag="LK694_002063"
     CDS             complement(6455..6856)
                     /locus_tag="LK694_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBK5052908.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6942..7148)
                     /locus_tag="LK694_002064"
     CDS             complement(6942..7148)
                     /locus_tag="LK694_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052909.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7243..8745)
                     /locus_tag="LK694_002065"
     CDS             complement(7243..8745)
                     /locus_tag="LK694_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBK5052910.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8763..9218)
                     /gene="argR"
                     /locus_tag="LK694_002066"
     CDS             complement(8763..9218)
                     /gene="argR"
                     /locus_tag="LK694_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBK5052911.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9316..9933)
                     /locus_tag="LK694_002067"
     CDS             complement(9316..9933)
                     /locus_tag="LK694_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBK5052912.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10154..11527
                     /locus_tag="LK694_002068"
     CDS             10154..11527
                     /locus_tag="LK694_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5052913.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11602..12714
                     /locus_tag="LK694_002069"
     CDS             11602..12714
                     /locus_tag="LK694_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBK5052914.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12786..14423)
                     /locus_tag="LK694_002070"
     CDS             complement(12786..14423)
                     /locus_tag="LK694_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBK5052915.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14591..15199)
                     /locus_tag="LK694_002071"
     CDS             complement(14591..15199)
                     /locus_tag="LK694_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK5052916.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(15583..>16108)
                     /locus_tag="LK694_002072"
     CDS             complement(15583..>16108)
                     /locus_tag="LK694_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBK5052917.1"
                     /translation="MNAQRCLSYQTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQG
                     KDFHFHEEMEPKVDEIYPKLKPMLSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALAN
                     LGDRTALPEISECLTDVRPVIRGTAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVIN
                     ELEHAQKLLRRKLK"
BASE COUNT         4475 a         3290 c         2782 g         5561 t
ORIGIN      
        1 atttttactc attcatagac acttccttaa ataactttta ttatagaagg tattgtaaat
       61 aataatattg acaaaaagtg actcaaaata aaaaaaaact tacattatac tggaatgaac
      121 tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat
      181 tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt
      241 acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat
      301 tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt
      361 tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca
      421 ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct
      481 ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg
      541 acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt
      601 tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa
      661 aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt
      721 aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta
      781 gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat
      841 tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat
      901 gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt
      961 taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt
     1021 cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt
     1081 aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga
     1141 gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc tttcttctga
     1201 cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac
     1261 tcggcctctt ccccaccact aaatggatct tcaatttttg gaactaacgt aattgttcca
     1321 tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa
     1381 ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc
     1441 actcccttaa taaaagtata ctatataaca ctaagtaacg caaaattccc ctttgtaaac
     1501 tatctagaaa attgttttgt ttaaagtata tcttattgga tataaataga aataaattgt
     1561 agccagttca ttttttcttt aactaaaaaa ataatccaag aaacattgaa cctatggatc
     1621 caaaagctac ccaaatacta tagctaaccc cccgctacct tcaattttgc agataaaacg
     1681 ctttgggaga aaatttcaaa gacaacaggc catcctatca tgcccgatta taaacaaaaa
     1741 gctggctatt ccttttgctc tgacaaattt caattgggaa agttcacgaa actagcaggt
     1801 gaagaagttt caactgttcg gatcaaagaa tgcccgcttc aaatagaaac attggttcaa
     1861 cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg agatcgtagc tatttttgta
     1921 gaagaaggga ttttacatga tccaactcat atcaatgtgg ataaatggca acctcttatc
     1981 tataaattca gagaatatac aactgccaac cagtcgttaa ctttgaattt tagattccaa
     2041 gaattcaaag agtcataatg ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac
     2101 gagtaaaaaa caaatagaag ctatacatta gaaaggtcga ctgtgaccct atagatgcag
     2161 tatatgcagc ttatgagtat ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc
     2221 tttttcgttt tttgccgctc tttctttgaa aaatcgatta tgttatacct tctccattac
     2281 taattcaggc atctgctgag taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg
     2341 ttcgatttgt tcttcagtca agacaagcgg cattcggtca tggattggtg cgactgtgcg
     2401 gttaggttca gtagtcatta tgattgattc tgccattttt tcatcctgat ggctacgata
     2461 aaaaccgcca atatacacta ttttttcctt atttgaaaac ataaatttgt ctttttcttc
     2521 attccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt
     2581 tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat
     2641 ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca
     2701 agatttgacg gaaaaacttc tccagctgct aattccgctg cctgcttcgg ttgtttcgtc
     2761 tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca
     2821 cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc
     2881 aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca
     2941 gtgaatggta ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag
     3001 aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct
     3061 gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta
     3121 gaaagctatt ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct
     3181 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
     3241 tatgagcagc tatttttatt tggctgttct tattacttga tgcagggcag ctttttcaca
     3301 acctctccac taaacgatgt tcaatcagct aaccttcggt attagctcaa aaaacggcaa
     3361 aatatgagga actattttgc cgtttgtttc tctaatactc aggtcaaagc gctgatttca
     3421 caacctctaa tacacggtgt tccagaagta gcttcttcga aaaataagcc gaaatttcac
     3481 aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg
     3541 tcacaacctc taattattaa agaacctttt gcgaagttaa caatctttct ttgatcaatt
     3601 gaaaattctg cctaagtagt gatagatcct gtgtgacttt caacaataag atacttccct
     3661 ggatttcagc aatcaacgct ttggcttgac ggtctgcttc ttcatcggat agtttttctt
     3721 cagaaagtgc caggatattt tgtttcgtat acttgaccca ttgattgact aattctagta
     3781 gcaacagacg gtattcttca tccactgtac tcaactcaat aatcaaatta cctacaggac
     3841 agccatgata agtgatctgt gtctcatgat cagcgaaaat ccagtccaac atttcttcta
     3901 atgcttcttc tggctgtttt ccagcattta agatgtcttc gaacaattct gtctgccata
     3961 cgtccatatg cgcatggatc accgctagac aaacttcttt ttttgaagaa aagtaataat
     4021 aaaattgacc ttttcctgta tcagaagctg ctagaatatc tcggattgac gtttcatgat
     4081 agcctttttt ataaataaga tctttggtcg cttccaagat cttttccttt gttttcaatg
     4141 atattctcct ttttcatttc tcatagtcct atattataca gtaaattcat cattttttct
     4201 tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac tataaaactg
     4261 ttctcatata gctagatgtt cattgattca agaactgcga ggacaaaagc aattcctatc
     4321 ttatgatagt tgctctaata cttcctctgt tgtcatcact tgatctgcaa taaagccaaa
     4381 gttcgtcaac gctgcatcca ttgcttcttg gccaggagca ccaattgcat ctgtcgctac
     4441 cacaacattg aatccactct ctaccaattc acgcatatgg gaatcgctac acaagtttcc
     4501 gttcattcca ccaagaatta ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc
     4561 attgctttct ggtccaaaga ttttatgagg agagctgatg atcgtatttt catccaaata
     4621 aggtttcaat tcttcgataa aatcggctcc tgaaccttct tccactgatt catattgact
     4681 ttttctagca aacatctgct gctcaagcat catatgttct cctgcaccgc caaattgcca
     4741 tttatggtca tgtgggtaaa aataatgagg agaaataaag agtttgtatc ctttttcttt
     4801 tgcttgtttt accatttcaa tcatattttc gatcgtattg tatttttcta agatatcttt
     4861 tgtcaaacca aagccaccgc ctgctggttt caagaactcg acttgcgggt cagtgagaac
     4921 tactgctgta tttttattat tgaacatggt ggattctcca ttcgttattt attttaatac
     4981 atcatttaga gcttcactca ttgtcggatg agaatagatt tgatctctca ataacgtata
     5041 aggaagtttt ccattcatag ctagcgaaat caagttgatg acttcatgag aatcttccgc
     5101 atagatactt gcccctagaa tcaagtcagt ttcagggtcg actaaaactt tgaatacacc
     5161 tttaggatct tctaatactt gtgctttagg gacaccgctt gtcatatact tgaataattt
     5221 gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa cctacattgg ataaagtcgg
     5281 agtgataaat acactgtaag gcacagcttt ccggttattc gttgttcgtt tattctcgcc
     5341 cttcaactga tccataatga ttcgataatc atctagagag atataagtga actgcaatcc
     5401 gcctttgacg tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt attcatccac
     5461 agcgattgct ccgcgatctg ttagtttaac atctgtattt tctaatccaa gtgatgctgt
     5521 acttggtttt cttccagttg cagccaaaac ttttgacgct tggattgtta cttcttggcc
     5581 atcttctgta aaggtgacgg cagctgttga ttcttggtct gctacttgat ctacagatac
     5641 acctagatgg aattcgattc ccgcatttgt catgtcttct aaaatcattt gcgcgatatc
     5701 ttcatcttca cgtggaagaa attcagaatg ggcatcaaga acgacaactt tagatccgta
     5761 ttcattgaac attgatgcaa attccaagcc aatatatcca gccccgatga tcactaattt
     5821 ttccggtaat tgttcctgat ccatcgcttg cgtagaatca atcaagtagc gacttgtatt
     5881 caatcctttg attggtaaaa tgattggttc agaaccagta ttgatgaaaa tccgttcgcc
     5941 ttcaacaatc actttttcac cattattcgt cacttcgatt tgatggttag aaagaaaacg
     6001 tgctgtaccg tccatcactg tcccagttgc ctcatcgctg atcatatgat aatttttggc
     6061 gcgaagcatg cccgtcaatt tttctttccg agcaacagcc tctgtaaagt cgattcctct
     6121 ttctccattg aaaatcaatg atttagaagg aatacagccg atgttgatac atgttccgcc
     6181 atacatacgt gttgactttt caacgactaa aaccgattca ccttttgaag ctaacgcctt
     6241 cgctaaagtc ttaccgccct ttccaaaacc gatcacaata ttttgatatt tttccatcat
     6301 ttttccctcg cttctttttt acctaccgat tggtacaaaa atatgataac cgataattgt
     6361 ctattctcca aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc
     6421 acgtttttag tctggttcga tttcctttat tcttctaaac aatcgttact tctacatcga
     6481 tatttcctct agttgctttg gagtaagggc agaattcatg cgcatcttct gcaatttttt
     6541 gggcttcttc ttcgctgatg ccatcgatat gcacttctaa tactacaccg atctgatagc
     6601 cgccatcatc taaattataa agagagacac gtgcttttac tgtggactct gctgtcattc
     6661 ctgcttcttt gatcatatgt tgtaccgccc cattgaaaca agaagcataa cctgctgcaa
     6721 ataattgttc tggatttgtt ccttctgcat ggattcctgg aggagtgatt ttcatatgca
     6781 tcgagccatt tggtgcctcg acttctccat tgcgcccgcc atggttgatc attgctgttt
     6841 cgtatgcttt tttcattgta ctgctccttt cgattgacca tttgctactt ggctataatt
     6901 atagtgtatg acaagtaagg cgctttctac aaaaattctg cttattcgaa taccagaatg
     6961 ccgtcttcta ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata
     7021 tttgttccaa aataaatagc aatcttttct acataatctt ttatatgatt gattttaaga
     7081 tacatatata ccagaaagtt gaccccataa accaatactg ttaaaaaaat aaccatattc
     7141 gttaccattt tttcctcctt ttgctggtct ccctaccagc actcgattta accataccac
     7201 tttttttgcc gtccaagcaa ctagcctttc aaaaagactg gtttattctg ctgttaatga
     7261 ttcaagatca gaaaaatgtc cgaataattt tgctaatttt actaacgcca acttgttctt
     7321 tccgaattca tcattttcta tctgtaatac tagtaacggc tcatgtaagc ttaaacggat
     7381 caaaaaccag ccgtttccgt atggctcttc tagatcatat cgaatacctt cttcattttc
     7441 cgggtcgaag ttcatccctt tttcttcttc caacagtcgc ttcatttctt ctaatatctt
     7501 gtttccttgt tcgcgtggtt catcgctgaa caattttaat cggatttctt gtacttctac
     7561 gggctgtttc aactctttga tttgttcatc taatgtgata ccttttcttt ttaattctgg
     7621 caaaagcatc aggatctttg cgatgacata tgcaccatca tccagaaaat agttttcctt
     7681 gaatgcagcg tgtccgcttg tttcgatggc tagctgtgta tctttcccaa gacggttaag
     7741 ttctaatgct ttattgatta catttcgata gccggaaata taacgaactt ggatgcctcc
     7801 tttttcttca atgaatcttt gtagatgaga agaagtaggg gaattcgtca cgattcttgt
     7861 tcctggatgc tcttttaata cgattgtact taatactgcg atcaaattat ttcgattgat
     7921 cactgttcca gaagcactga caactgcaga acgatccaca tctgtatcga aaatgatccc
     7981 aagatctgct tcatttgcta aaacagcctg cttgatactt tccatcgctt ctttattatc
     8041 aggatttggg atatgattag gaaatcggcc atctggttcc aaaaattggg aacctgttga
     8101 atccgcacct aagctattca atactttttc agcaaagaat ccaccagcac catttccggc
     8161 atcaacaatg attttccaac catttagtgg tttgctttct agtgttccaa tccccttttt
     8221 aatttttgtg actaaatctt gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc
     8281 agtttgttcg ctttttattt tttgctgctg tgataggata aaagcaatat cttctttttc
     8341 tgctcctcct tctgaggtaa agaattttat tccattataa taatagggaa gatggctagc
     8401 agtcagcata accgcagcat cgcacgagaa ctgttcaaat tgagtggaca taaacatagc
     8461 aggcgtagta gctaattcga aatcgatgac atgtatccct tgttctttca gactttggat
     8521 caacgcctct ttcagtgctg gtccgcttat cctactgtca tgaccaatcc caatcgtcca
     8581 ttttttattt ggatcggttg atttcttctt tttctttatc cagtttgcta gtccccagcc
     8641 gatctttttt accatgtctg tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat
     8701 tcctcgaata tccgatccat tttgcagcgc tttcatatct ttcatataaa agtcctcctc
     8761 atttattccc tcattttttc cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg
     8821 caaataataa tgaatgtatc tacaccggcc atcgttccga caacttcagg gtaagccact
     8881 tcatctagcc aattcgttac cacatcggca ccatttcgtt cagtcaatac gataaccatg
     8941 aactgaacag actggatacg caaaacctcg cgtctaattg ctgtacgcaa acgttcttca
     9001 tttaaagaaa cagatggctg gttaaataat gtatagcgta ctattttgtt ttcattttga
     9061 ctttttacta acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg
     9121 cctgcttcat gcagccgcgc aagcagctct tcctgtgtcc caataggttg ttctgtaatg
     9181 atctggcgga tcagtgcttg tcgctgtttc tttttcatat tagacacctc tcaatgttga
     9241 ttatagcaaa agataagtaa tatttcttca tattttggta ttttttactt ttattttttg
     9301 ttttcattca ggttgctaac ctttttctat tttaacacgc aaaatcgtct tcattttttc
     9361 aggtgctgat tttcttggat cgctgatgta aatctctttg tgttctttag aagctctttc
     9421 atagccttct tttgcgatga atgcttctaa tttctcaaac gttgctggtt cttcggcata
     9481 gggcccgacg tgtaagattt gggcacaaag accttcttcc atctttttga atactacttg
     9541 ttctttcaag tcttccggaa gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc
     9601 tgtaacaaag tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt ttttcatcac
     9661 tggttcatgc aaatactttt cttgcagtcc ccattgtcct tccagtggat agacagtata
     9721 catctgataa tcaggaatca gccaattttt tttaggcgac atgcgtattg catagctgac
     9781 tgcatataga caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct
     9841 gatgctaaag aatgattttt taggaacctc tagtatttgc gggctttttt tcatactata
     9901 gctcttgtct ttacgccatt caattttttc catttttctt cctccgcgaa attccattta
     9961 gttatttatt atcatacctc tttttgagtg acttggctaa tcgtgttgac actatttttc
    10021 agtttgtgct agaataagtg aagtaaataa atcgtacaga taacacgttt cggcgtgtcg
    10081 agagagaggc ggtttctatt tttataatag aaatcactct ctttttttac gaaatttttt
    10141 agaggtgatg taaatgacaa aacaaaaaca gctgctttta gtacctgcag taattgcaac
    10201 agggatgatg tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa ctttcccaac
    10261 tcttatcaga cagtttgatt taacgactgc acaagtccaa tgggtaacaa cgatttatct
    10321 tttgatgatt tcgatcattg ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa
    10381 aaaactcttt ttagcttcaa atctgctttt cttggcaggt ataatgatcg atttcttctc
    10441 tcctaccttc atggtcctat tgtttggtcg attgctccaa ggtgcagcga caggtatcgc
    10501 tcttccgttg atgttccata ttatcttgac atttgccccc ctcaatcgac gaggcgcgat
    10561 gatgggtgtc ggtactttga caacggccat tgctccagca attggcccca cttatggagg
    10621 cattatgact tcacatttta catggaatca tatcttcctg tttcttgtgc ccgttttgat
    10681 tctttctttg attatcggct ggtttgctat tcccgagatg gcagtacaaa aaagtggtta
    10741 tttggattgg ataagcgttt taggaatttg tcttttgttt agtggaagtc taacattttt
    10801 gagtacgttg ggaacagttt ccagctggat tgctttaatc atcggcggta ttggctggtt
    10861 ccttttttac tatcgaagta aaaaagtgga gcatccactg atccgactgt ctattctttc
    10921 aaataaaaca ttccgcttgt ttttgattgg cttcttagtt tatcagtttt tattacttgg
    10981 tgtatctttt gtcttgccga atttccttca gatcgttcaa ggaagtagtc cttttgtcgc
    11041 tggattagcg atgcttcccg gcgcagcggt tggtgctttg ctttctcctt tttccgggaa
    11101 aatgctggat caatatggtc cgaagaagcc aatttttgct gggctgattc tttctttgat
    11161 tggttggcta gcattattcc tactcatagg acacgcttca gtgggtgtat tgattgcttg
    11221 tcatgtgttt tatatgatcg gtatcggatt atcttacagc aatatgatga ccaccggaat
    11281 gaatgcactg aatgaagaac tacaaggaga cgggaacgca atgttcaata ccttgcagca
    11341 gttttctggc gctgtggcta cttcattagt agcggtcatt atcaattacg tccagcatca
    11401 tacttcccat aactatgaag tgtctactac attaggatca aaagcagcat taggcgtctt
    11461 gttgctgttg cttctcgtaa gctttgctcg attcgcctat tatctgttct ttgctaaaaa
    11521 agcgtagatt ccatatacgg caggtgttgc atctgccgtt tttttgtgtg aaaatggttg
    11581 tttgtaaaag gaggcttttt tatggaaaaa attgccatta tcggtggcgg gatcattggt
    11641 atgaccctag ccagttactt agatacatca aaattcaatg ttgctttatt tgatgaaggg
    11701 acaggacagg caactaaagc tagcgctggg attatctccc cttggttgtc gaaacgcaga
    11761 aacaaacaat ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa acttataaaa
    11821 gatttccaac ttgatcaaac gatttacgca caatcaggga cacttttgct tcgacctgaa
    11881 aaagatttag ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt
    11941 ggaaacatcg aatggctttc acctgaacag acatctcaag cacttccgtt attgaaacca
    12001 gcagtttcac tgcgtatcag tggtggtggt cgtctggatg gaaaagcata tttagaacat
    12061 cttggtcagc gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt cactttgcat
    12121 aagaagagcg attcttggct catcaaggga ccgtttggag aatacacagc tgatcgtgta
    12181 attttgacac ctggcccggc acttaagcct ttattacaag aattaggcta tgctgcagat
    12241 atacgtccgc aaaaagggca gctcttagca tttcagacat cttataagat gagtcaagac
    12301 tggcctgttg ctatgcttga cggagaagct gatttcattc ctttcatgga tggaaagatc
    12361 cttttaggtg ctacccatga aaacgatcaa ggctgggatt tggctcctac tagcgaagct
    12421 tataagggat tgactaagag tacagaacag ttcttgactg atccagatca gataaaaaac
    12481 tggccatacg agttccgtgt aggtacccgt gcttatacgt cagatttttc cccatttttc
    12541 ggcgtactgc cagatgatcc aagcttactg gctgctagcg gtctaggctc ttctggcttg
    12601 accacaggtc cttatatcgg ctatctatta gccaacttct taaatactgg tcattggcct
    12661 acggaagatt atcaaaagga catctccacc tatattacga aaaatccatc atagaaaaac
    12721 agctttttca agtaaggatc ccgagcgata ttgcgcatgg attacttctt gaaaaagctg
    12781 ttattttagt tcaatttctg aatcaaattc tccaaacttt cttttgcttc atctggcaag
    12841 agatcttttc ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc
    12901 agatttttat aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg
    12961 atgtcttcaa atggaccaaa cgacttgaat tctgccgttt gttgcacgat tctttcaacg
    13021 attgacaatg tcgtttcagg tttgattttt ttatcatcga aatatccagt attcaggaca
    13081 tagcaggctg tttgctgttt caaaaacaac tctcgaaaag cttgatagtc ttccctaaga
    13141 ggatagacac ggaaaggatt agcaaatggt tcaatgacta gctcatctag ctgtcttttt
    13201 gtagtgatat tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct
    13261 aaaactgggt cattgatctt gataattggc ggaagcgaat catctttcat gatccaaaaa
    13321 acagcatcga tcttttcgct aagatgatcg actcggtcgg gtgtcgcata acgtgatttg
    13381 actgtccgcc catttccgtt tcgtatatct cctgttacta ggactttttc tcctttactt
    13441 gtacatgtca cgcccacatt ctgacaagta aggaaagaag aaatggccgg atcatcagac
    13501 ggatagtctt gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa accatttttt
    13561 cgagaaataa cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt
    13621 gctaaggtaa tcgttgattt tccagaaccg gacaacccaa atacagccat cgtataggtc
    13681 tggtcattac tagcatattg tttcatccca ccgtggcagg caataaagcc attgcgattt
    13741 cccaatgccc aagcaagcgt caaagtcgct ttttttaatt ctccaaagta acgtaatcct
    13801 agcacaaccg ctgtattatg gagagcatca aacaaaatga gtccatttgg aaatgtttca
    13861 tgtgtccatt cgggatcaca aaagatataa agatctccct catcaaagga aacagacttt
    13921 tgataagtct ctaagatctt cgaattcgct ggctgaaaat tcagcaaata agaatacaaa
    13981 ttgttctcaa atccttctgg aatcagcaaa tgactttgca cagaaaattc ttcagatagg
    14041 ccaacgatta cttttgtatg gtaaaactgg cgcttgcgac tttgatacac agcttctctt
    14101 aaaattttct cgtatttgct ttgttccttg tctaagtcct caaagatccg ccgagcactt
    14161 gctgttcttc ccacaatgct tccatcattg aatactagtg tcctaacatc agctggtaaa
    14221 tctagttctt cagcatgtga gattgttaaa tctgtgatga tcgttcctgg actccctttt
    14281 gctagttgat aagctgtctt cctatcaaaa acttcatgaa cattattacc gtaaaatgtc
    14341 gtttctacaa tcgatcgaat actggataac agtggatttc cacttgtgat ttcgtttaat
    14401 tcgagactct cttttgtact catggctcct cctcattccc taaacaaaaa aacgttttct
    14461 ttattataca ttattcgagt ttttatttgt actgctgcgc tgtgggaagc aaacgagtta
    14521 tttttcacac aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc atcattttcc
    14581 ttttatcggc ttagtaccag ccgtttgcta accagaatgt tttagcagca tcccatgaac
    14641 cataacgaga tgttacatat tgttctgcta cacgttcttg attggcagct gaatagtcgc
    14701 cgtttaagta tgaagcatct aattgataac ggccgatgta acgtccgtta gtagctgtgt
    14761 atgaaccgcc tgattctttt tgtgcgatcc attcttttgc agagcttgaa gttggcgctg
    14821 ttgcagcagc tggggcagca ggagctactt caactgtttc aacaggtgca acgtatgtat
    14881 gttcttgcgt tgctgtttca gttgtagatg ctgtattttc tgttgttttg ttttctgttg
    14941 ttgctgaaga atcatttgaa gttgggattg ttaattgttc accaacataa atgcggttga
    15001 tgtttgcgat cgaatttttt tcagcaattg catcgatcat tgagttgtct cctgcaaatt
    15061 tttgtgagat cttagataat gaatcacctg attttactgt ataaacttca tcagcgtgag
    15121 catttgtagt tcctacaaac attgcagtag caccagcagc aaaagttgta cctaaaacaa
    15181 ttttcttaaa tgaattcata ttaaactcct ttttttctcc gttttttatt ctttcgacgc
    15241 tattaacttt atcatttaga aatatttcat aggtaaaaga aacatgacat ttaatgacaa
    15301 aataacgaat tatttaatat ttgtaacatt taaacaaata tttttttgat acaaaaccat
    15361 aaaagccttg ttgtataagc aaagtaatcg tttttctatg tttttttatt tttgaatgaa
    15421 aaacgcagat tctgtaacat tcactgcaat aatccataaa aaaaagcaaa aaaatttttg
    15481 atttttatca aattctctta aaaaaatgta aaaaatacaa aaaaaccagt aattgttaca
    15541 acaattactg gacaaaaact ttgaataatg ttaactattt ttttatttta actttctccg
    15601 caataatttc tgggcatgtt ccaattcgtt tataacgact tcttctgttt ctttagctaa
    15661 tatttcatct aatacatcaa gccatttttc cggttctttt gttcctagac gtccaatagc
    15721 ccaagctgct gttcctctga tgacagggcg gacatctgtt aaacattctg aaatctcagg
    15781 caatgccgtt cgatcaccta gattagctaa agcaatcaat gcgtttctct gcaaaggttt
    15841 cttcccacgc catgaaccag ctaaatgccc aaattgctgt ttgaagtctt tattagacat
    15901 cgaaagcatt ggctttaatt ttggatatat ttcgtctact tttggttcca tctcttcatg
    15961 aaaatgaaaa tcttttcctt gattgtaagg gcagactaac tggcagatat cacatccata
    16021 aatgacattt cgcatttttt tacgatattc ttccggcatc atcccttttg tctgagtttg
    16081 ataagacaaa catcgctggg cattcatt
//
LOCUS       DAFBQH010000063        15988 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.065, whole genome shotgun sequence.
ACCESSION   DAFBQH010000063 DAFBQH010000000
VERSION     DAFBQH010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.065"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            37..555
                     /locus_tag="LK694_002073"
     CDS             37..555
                     /locus_tag="LK694_002073"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBK5052918.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            683..1366
                     /locus_tag="LK694_002074"
     CDS             683..1366
                     /locus_tag="LK694_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBK5052919.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1675..1748
                     /locus_tag="LK694_002075"
     tRNA            1675..1748
                     /locus_tag="LK694_002075"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1709..1711,aa:Arg,seq:tct)
     gene            1907..2266
                     /locus_tag="LK694_002076"
     CDS             1907..2266
                     /locus_tag="LK694_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBK5052920.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2389..4020
                     /locus_tag="LK694_002077"
     CDS             2389..4020
                     /locus_tag="LK694_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBK5052921.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4082..4723)
                     /locus_tag="LK694_002078"
     CDS             complement(4082..4723)
                     /locus_tag="LK694_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBK5052922.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4909..4985
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5005..5313
                     /gene="rplU"
                     /locus_tag="LK694_002079"
     CDS             5005..5313
                     /gene="rplU"
                     /locus_tag="LK694_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBK5052923.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5340..5684
                     /locus_tag="LK694_002080"
     CDS             5340..5684
                     /locus_tag="LK694_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBK5052924.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5697..5990
                     /gene="rpmA"
                     /locus_tag="LK694_002081"
     CDS             5697..5990
                     /gene="rpmA"
                     /locus_tag="LK694_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBK5052925.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6424..9231
                     /locus_tag="LK694_002082"
     CDS             6424..9231
                     /locus_tag="LK694_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK5052926.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9422..9913
                     /locus_tag="LK694_002083"
     CDS             9422..9913
                     /locus_tag="LK694_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5052927.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10016..11005
                     /gene="manX"
                     /locus_tag="LK694_002084"
     CDS             10016..11005
                     /gene="manX"
                     /locus_tag="LK694_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBK5052928.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11038..11841
                     /locus_tag="LK694_002085"
     CDS             11038..11841
                     /locus_tag="LK694_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBK5052929.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11862..12776
                     /locus_tag="LK694_002086"
     CDS             11862..12776
                     /locus_tag="LK694_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBK5052930.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12859..13233
                     /locus_tag="LK694_002087"
     CDS             12859..13233
                     /locus_tag="LK694_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBK5052931.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13372..13617
                     /locus_tag="LK694_002088"
     CDS             13372..13617
                     /locus_tag="LK694_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK5052932.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13789..14853
                     /locus_tag="LK694_002089"
     CDS             13789..14853
                     /locus_tag="LK694_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBK5052933.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15099..15524
                     /locus_tag="LK694_002090"
     CDS             15099..15524
                     /locus_tag="LK694_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBK5052934.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15517..15969
                     /gene="nusB"
                     /locus_tag="LK694_002091"
     CDS             15517..15969
                     /gene="nusB"
                     /locus_tag="LK694_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBK5052935.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELGLFVK"
BASE COUNT         5117 a         2645 c         3487 g         4739 t
ORIGIN      
        1 acttctttct ttgaataaag aaagaagttg aaaacgatac ctgattacaa aaaaataact
       61 tttataagtt ctataacgtt gattatattt ttaattctta ttcttctcgt cgtcttggtt
      121 ccccgaattt ttggtttgac tccttatatt attgaggaca aagaaatgag tccatattat
      181 gcacaaggaa atctgatata tgtacgaccg aaaaatacaa atgagatttt agtcggagat
      241 attattacat attatgaaaa ttcaggaaaa aaaatctcga cgcgtcgcgt aatagccgtt
      301 gaaaacaacc acgaagattt ttatacaaaa gctgatacaa agacttatat tgagcctgga
      361 gtagtaaaaa agagaaatat tatcggtagt cccatctttc agattccata tattggacta
      421 gtcctttcaa aaacggcatt tgcatggatt caggggttat tctttacgat tgctttttgc
      481 ctaacaggaa tcacagcatg gaataccttt gtagagttga aaaagaagcg gagacgaaca
      541 cagaaatttt tgtaagaatg ttaaaaacat aaatgtattc cagagatcca acgactcttc
      601 tgaacatgaa acacgagaaa acactcatgc agcttgtaga tttagtcagt tgttcgtata
      661 atgtgtgaag aggaggagta gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga
      721 agaactattg gattttatgc gtacccagca aaaaaaatta ccgggtgagc taggcaaact
      781 ggaagaagaa gctcatgtag cagaagtacc agttattccc catgaaacag ttgtattttt
      841 gaaattttta cttggacagc ttcagcctga acgaatcttg gagatcggag cagctatcgg
      901 tttttcatca agtgtcatgg ccacgacaat cggagaaaac gctcatgtta cgacaattga
      961 tcgtttcgat gtcatgatcg agaaagctaa aaaaaactat gaacgtctag gactgacaga
     1021 taaagtgact ttgcttgaag gtcaagcagc agatatcttg ccggagttgt ctggtccgta
     1081 tgactttatt tttatggata gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt
     1141 acgtttgtta agaaaaggtg gcgttttgat ggtagatgac atcttccaag gaggaacgat
     1201 cttgctccca gatgaagaga ttccccgtgg caaacgagct atccatcgta aattaaatga
     1261 atttcttcgt gttgtgatgg atcatcccga tttgacatcg acaatcttgc cattaggtga
     1321 tggtgtgatc ttaatgacaa aggaatcaga aacaatcgag ttgtgataca tgattatggc
     1381 agcctgtaga taatcgattg aatcgagggc tcaattcgaa cttgatgtaa aagtttctat
     1441 atttgtaagt caattaaagg atacaggaaa tctaaataaa caggagtagc atgaagtcaa
     1501 tgtagctcct gttttcaatt gataacctta gaacagctga aggagaatgt ctcgttttaa
     1561 aagattgatt tagtagttgg gccaaaataa ttcattattt gctaattaaa taactatttc
     1621 gtattgacag aagcaattta attatctata attattgatg ttccgctgat tcttgtcata
     1681 gtagctcagc tggatagagc atccgccttc taagcggacg gtcgggggtt cgaatccctc
     1741 ctgtgacgct aaaaaaccgt tagaaactga ggtttctaac ggtttttctt tgttttttgt
     1801 gccaatattg tttgttttca aaggcaaata aaataaaaaa acatccttat cgagtacaat
     1861 tataaggagc aaaaaaataa aactcatcag aaaagaggaa gaagatatga cgatactatc
     1921 tagaatttta gtgactctag ttgcaattga atttttttat atcatgtata tcgaaacggt
     1981 gagaactgat tctgacacga caagtagggt ttttaaaatg agtaaagaag agttaagcag
     2041 aaaatctgtt caaacattgt ttaagaacca aggagtgtac aatggactat taggagtggg
     2101 actactttat ggagcttatc tttcttccgc atctaaagaa atcacaagta tgctattaat
     2161 atctattttt tttgttgcat tatatggcag tttgactagc gatcgttgga ttttactcaa
     2221 acaaggtggg ctagctctgt tggcattgct ttctattttc ttataaatat agaaaaaata
     2281 agaatttatc taacaggaaa ctttgtgaaa aatattactt tatctcatga aatatgatat
     2341 tctaatgaat ggtattttag aaaatttcat gaaaaaggag ttagataggt gttcaaaaat
     2401 tatgtttcct cactaaaaaa agaattttca ggttatgatc tttcaagatt tcagaaagat
     2461 cttttggctg ggatcacagt agctgctgta gcattgcctt tagccttggc ttttggggta
     2521 tcaagcggag ctgatgcggc cgcaggtttg attacggcta tcattgccgg tatcgttatt
     2581 ggcggtttat caggcggttt ttatcaaata tcaggtccga caggtgcgat ggcagctatc
     2641 ttgatgtcta ttgcggcaac aaagggaatg caaggtgtat tgcttgctac ttttttagcg
     2701 ggtctatttt tacttgcagc aggacttttc cgattaggaa cactgacttc ttttatccca
     2761 gcacccgtaa ttacggggtt tacttcaggt attgctatca ttattgcatt aggtcagatc
     2821 gataatttat ttggtgttca ctcggaaggt acaaacgtta ttgaaaaact aagtagttat
     2881 caaacgatag gatttccgat aaacacagcc tctttgatca tgggagggct agtcatttta
     2941 gggatgttta tctatccaaa aaaatgggcg aaacgaatgc ctagttcgct gctggctatc
     3001 attttggcaa cagcagttat gctattgttc catttaccag tagcaactgt gggtaagatt
     3061 ccacaaacat tgatcagtag taatcgtttg aatatgggag attttagctt ttctgccttg
     3121 caacaagtgg cagttccagc tattagcatt gctttattag gaatgattga aagcttgcta
     3181 tgtggtgctt cggctggccg aatggcaaat aagcccttag atagcaatca agaattaatg
     3241 gcgcaaggaa ttggaaatct gttgcttcct ttctttggcg ggattcctgc tactgcggcc
     3301 atcgcccgta caagcgtggc catcaaatct ggtgcacaaa ctagattagc tggtatgata
     3361 catgcagttg tcttactgct ctccatgctt gtttttgcgc cagtgatgtc acagattcca
     3421 atgccagcac ttgcaggtgt tttgattgtg acggcttggc gtatgaatga atgggaaacg
     3481 atcaaggagc tatttactaa taaatattgg tcagcagttc tattgtttat ccttacgatg
     3541 atttgtacag ttatctttga tttaagtatt gctatcgtga tcggggtgat cggcggctgt
     3601 gtattttttg ttgtcaaaag cgctgctatc acgatttcat tagaaacgat cgattggcaa
     3661 agaatggggc tgccagaaac ggaaaagctg aataactgga cagtagtgta tattagtggt
     3721 ccactgtttt ttatgtcttc agaaaaactc aaaaataaat tggaagaact aaatcatcac
     3781 gatggtgtca ttttctccat gcgaggcgta ccaagtattg atttgacggc acgtgcagtt
     3841 tttgatgaat ttcaagaaaa agcagagcaa agaggacaaa cgattatcta tacttccttg
     3901 cagccagaag tagaaaaaca actttctcac ttgtgggaac atacattcac gcatcaacat
     3961 caaacagttg ctcatgcgtt gacagcttta catgcgaaat atactgcaat tagccattaa
     4021 aacaatgaaa aagaacagac aagactgtaa actgaagatt ttatggtctt gtctgttctt
     4081 tttaatattg ctgtaaaagt gaagctagta cttgccagtc ttgatggata acaggtacaa
     4141 gggagcgatt gtagaaaata gctgctggat gaaaaagtgg aagaattcga tagttttttt
     4201 ctgaccattc gtaggcatcc ttttgttctg ttaattgtaa gacaggccct gtatagagtt
     4261 gaccatgatt tttggtaacc gtatattctt tgccaagtaa acgttgaaga ccaatgtttc
     4321 caagtgtcgc aattaatttt ggctggacct tcatcagttc gtaatccaat attggtgcat
     4381 gcgcaaatac ttcggaacga gtaggtgtcc gatttggata aacagtttct gactgttgtg
     4441 tccgtttatt gatccgatga gtcacgcgat acgggcggct ccttacagca ctggtgatat
     4501 acacctcttc tcgtgttaag ccagttgaag aaagtgcctg catcaattct tttccagctt
     4561 gaccgctaaa aggaacatgc gtatcaatct ctgttttccc tggggcttct cccacgagca
     4621 tcagtttagg atgaagaggt ccttgcccag cgataaatcc ttctacagga cggccgttca
     4681 ttcttaaaga agcttcttgt atcagttctt ttgggtattc catttatgct cctccttttt
     4741 cttttattat aagaataaaa gaaacacaat gcgactattt tatttggtag aactgtgagg
     4801 atagaaaagc agaagtattg cttgaatttt tgttttactt tttgatttta aatttagttg
     4861 actaacggtt gctgttctgg taagctagta ctgttgtaat tgtggactcc acagctacaa
     4921 ccgctcagaa caagtaataa gcacagcata tgctgtcact tcggatggcg agtctaagta
     4981 tatcagaagg aggtgcttta tagcatgtac gcaattatta aaactggtgg taaacaagta
     5041 aaagttgaag tgggacaagc aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa
     5101 gttgtttttg acgaagttat cttagtaggc ggagaatcta caaaagtagg cgctccaact
     5161 gttgcaggtg caactgttga aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt
     5221 actttcaaat acaaacctaa aaaacattca caccgtaaac aaggtcatcg tcaaccatat
     5281 acaaaagtgg ttatcgacgc gatcaacgct taatagcgtt tgagaaagaa ggcctatata
     5341 tgatcaaagg aacatttaag cgaaatgacg caggtcagat cgtttcattc actttgaccg
     5401 ggcatgctga tgctggtcct tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag
     5461 cgatcagcac tgtcaacggg attgcctcat tagccggttt tgagccgatt gttgaaatga
     5521 atgaagaaga aggcggttat ctttataccg aagtcacttc aggaatgaca caagaacaaa
     5581 acaatattgc tgagattcta ttagaaaatc ttttattagg attacaatcg atagaagctg
     5641 aaaactcaga atatatccaa attaaaacaa ttaatgataa ataggaggtg cagaccatgt
     5701 tgttatctat gaatctacaa ttatttgccc acaaaaaagg tggcggttct acatctaacg
     5761 gccgtgactc agaatcaaaa cgtctaggtg ctaaacgtgc tgatggacaa acagttacag
     5821 gtggatcaat tctttaccgt caacgcggta caaaaatcta tccaggtgta aacgtgggta
     5881 tcggtggaga cgacacttta tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag
     5941 gccgtaacaa aaaacaagtg tctgtttatc cagtagctca agaagcttaa ttgaatctta
     6001 tgattaaaga ggttgtgaca agacttttgt cacagccttt tttgtttata aaaataaaag
     6061 ttgtagtgga ggctacgaag tttactttgt ctttttacac ttaatatttt tttgccaatt
     6121 tggaatcagg atgttttgat aactatcgtt ttcaattgat ggaagctata ttgtttgcta
     6181 tcagtgaaat aattatatgg taaacagtgt tctttttctt gtttccatta taaaaatcgt
     6241 atcgggattt ttcgttcttt taaattgaac atttctatct atccgcgtgt tatcaacaaa
     6301 acaaaatcag gtaaatagtt gacgaaacag aaataaatac atagtattat gttttgaagc
     6361 ctaagtacaa tagtctatag aaaaggagcg ataccattgg caaaacgcat tgaccgaatt
     6421 tatatgtatg tcaaagaaaa aaccgctcat ttaacaaagg cagaatatga tcaaggagta
     6481 acaacacagg aaattgctga agtgttaggg attcagcgaa caaatagcag taaagatctg
     6541 aatcagctag tgcgcgaagg aaaactgata aagacagacg gacgaccagt acgttacata
     6601 tatcaaacac tcgttacgca cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa
     6661 acgccggtct ctgaagaaaa aaatacactc caaatagata ctaaggatat ctttgcaaaa
     6721 atcattggag ctaacggcag catgaaaaat tcagtggaac aggcaaaagc tgcgattttg
     6781 tatccgccaa gaggattaaa ttgtttgatt acaggaccta ccggatcagg aaagacttat
     6841 tttgcacacg cgatgtatca ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa
     6901 ctgattgttt tcaattgtgc tgattatgcc aataaccctg agcttttgat gagtcattta
     6961 tttgggtacg caaaaggtgc ttttacaggc gcagatgaag aaaaaatggg gattatcgat
     7021 caagcggatg gcggtatgct gtttttggat gagattcacc ggttgccgcc tgaaggtcaa
     7081 gaaatgatct tttactttat ggatcatgga acttacagtc gtcttgggga aacaacgaaa
     7141 tcccatgaag caaatgtacg gatcgtaggt gcgaccacag aagacccagg ttcttctcta
     7201 ttagaaactt ttgttcggcg tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt
     7261 cctgcaaatg agaaagtcga tttagtaaaa atcatgattg cccacgaagc aaaccgcacg
     7321 cagcgaaaga tttctttgac agaagatgta gcaaaagcat tgatcggcag tgtgacctat
     7381 ggaaatatag gacaattgaa gtcaaatatc cagttagtat gtgcacgagg atttctaaat
     7441 catatgcaat cacccgaaat ctcgatcaca atcgatgatt tgacagaagg gattcgtagc
     7501 ggattgattc aattagcaag caaccgcagt gcaatggcag agctttctaa aatattggag
     7561 ccaaagataa ctgtagcacc aaatgatacg atcatgaaaa tccaatcgga ctcctatgag
     7621 ttaccttata atctatatga tatcatcgga gataaagcag ctttactgaa gtcggatggt
     7681 ttggatcaag aagcaatcaa tcatttcatt tctacagata tcaacatcca tctgaagtct
     7741 ttctataaag accatggttt ttcttttaat gcagacagca aattagcaga atttgtagat
     7801 ccgaaaatca tagaagtgac caatcagatt tatgtaatgg tcaaaagatc tttaccatat
     7861 gagtttcagc aaaactttat ttatgctatg agcttgcata tcagttcttt tttgaaaaaa
     7921 atcaacttag gagaagaacg gcatacgaat gataacatcc gtgagatggc ttttgatttt
     7981 ccagatgaat atgctgttgc gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg
     8041 gagattccag aaagtgaaga ctattacttg accgttctac tcgtctcgtt acgggcaaat
     8101 caagcaagcg gcagaatcgg tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt
     8161 atggtccaag ttgtccaaca gttgttagat gcagagaacg tacgagcagt agacatgcct
     8221 ttggatatgg atccgaaaac agcattggac cgaattgaac gcaatgtaca agaagtagat
     8281 gaaggaagcg gggtcatact cttagtagat atggggtccc ttgcgtcgtt taattcgcaa
     8341 attcaaagag acactggaat tccagtacgt actgttgata tggtaacaac ttctctagtt
     8401 ttggaaactg tacgaaaggt ctcagtatta gggacagatc tagatatgtt gcatgaatca
     8461 ttgaaaaatt ttcgaggtta cgcagaaata tcttctgtag aatctgttca ggaagcaaaa
     8521 cctattcata agaaaaaagc tgttttggct atctgcgcat ctggcgaagg tacagcacaa
     8581 agaatcaaag aattgatcga acgagcggta aacaagcgtc aagaaacaga attagaagtt
     8641 ctagctttgt cgattgtgga agtgaaagaa gaactgccag ttatccaaaa agaatatcag
     8701 atcatagcta ctactgggat tactgatttg aatatccaag ccccatttat tcctttagaa
     8761 cgttttattg atcaaaatat cgaagtcatt ttggatcagt tgctgatgga atcagaattg
     8821 gaggaaactg aattcatctc cctagatgaa gagtcagcta aaaatacgtg tgttgagttt
     8881 atctctgata acttcatctt tattaatggt agtaagttaa tcgatccaat gtggcaattt
     8941 tctacacaaa tctctcagac gacgggaatc ggcgatgaag aatatggatt caaaatcaac
     9001 ctagtgatgc acacagcagg aatgatcgaa cgtattattc ggaatgaacc attgactgtg
     9061 gaagaaaacg aattgacaaa tacaacgaat gatcctcttt atagccaatt ggcagcctca
     9121 gttgttctgt tggaagatca gataaaagta aaagtgccga tagaagaaat gtattatctg
     9181 ctacgtctag tccataatca gttagataaa aaagaataca ctgtgccatg aatacacaaa
     9241 aacgatacac ttttatagtg tatcgttttt gtgtattggt taagaatcct ttaaattcaa
     9301 ttagtgtatc gtaaaaaagt tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg
     9361 gttgttcaca cagaaatgat aggggtttct gtgaagtagt gaggaagaag gagttttgca
     9421 gatggatatt cgattggcac gtatagatga tcgtttaatc cacgggcaag tcgcaacagt
     9481 ttggtcgaag caaacaggtg ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga
     9541 taaattgaga aagtttctac taaaagaagc tgcaccgccg gggatcacag caaatgtaat
     9601 aaccgtgcag aaaatgattc aaatctatca tagtggatta ttatcacgag caaaagtgat
     9661 gcttttattc accaatcccc aagatgttga agcggttgtc cgctgcggtg tacagttgaa
     9721 gtcgttgaat atcggcggta tgagttttac tgaaggcaaa tcaatgatta ccaattttgt
     9781 ttcagtaaat caacaagata ttgattcctt tcgttacttg aacagtcaag aagtggagtt
     9841 agagattcga aaagtacctg ctgacaggaa aatccttttg atggaactac tagcgaaaat
     9901 caaaaaggtg tagcaagaat ggttgttgga aaaggtcaaa taattctgtt gtaatggtta
     9961 gcaattatta atatttttga atttgttaca acaaaagtaa ataggaggta taaatatggt
    10021 agggattatc ctagcaagtc atggacaatt tgctgaagga atcttacaat ccggttcaat
    10081 gatctttggc gaacaagaaa acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga
    10141 tgacttgaga gccaaattgg aagaagcggt tgcttctttt gataatcaag acgaagtgtt
    10201 attcttagtt gatttatggg gcggaacacc gttcaatcaa tcaaacacat tattcgaaga
    10261 gcacaaagat aaatgggcaa tcgtcagcgg tttgaattta ccaatgttga ttgaagccta
    10321 cgcatcacgc ttttcaatgg aatctgctca tgaaattgca gcacacatca ttgaaacagc
    10381 aaaagacggt gtaaaagtaa aaccggaaga attagaaccg gcagaagcac cgaaagcagc
    10441 agttgaagac gcacaaccta aaggagcact tccagaagga actgtggtcg gagacggaaa
    10501 aatcaaatat gtattagcac gtgttgactc acgtctgtta catggacaag ttgcgacagc
    10561 ttggacaaaa gcagtgcaac caaaccgtat tatcgttgtt tcagatgcag tatcaaagga
    10621 tgatctgcgt aagagactaa tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat
    10681 tcctattagc aaaatgatcg aagtagcaaa agaccctcgt tttggaaata ccaaagcatt
    10741 gcttttattt gaaaatcctg aagacgtatt gacagctgtt gaaggtggcg tagacatcaa
    10801 agaattaaat gttgggtcaa tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt
    10861 gtctatggga caagaagacg ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt
    10921 cgatgtacgt aaagtaccta atgattcacg cgataacatg gatgatattt tgaaaaaagc
    10981 aaaagctgaa ttagcaaaag cgtaataaat aatattaaaa tttataggag gcttatcatg
    11041 tctattattt caataatttt agtcgtatta gttgcctttc tagctggtat ggaaggtatt
    11101 cttgatgaat ttcaatttca tcaaccacta gtggcatgta cattgatcgg tttagttacc
    11161 ggtaacctcg aagctggtat cgtacttggc ggtacgcttc agatgatcgc tttaggttgg
    11221 gcaaatatcg gtgccgctgt agcaccagat gctgcattag catcagtagc atcagcaatt
    11281 attttagttt taggtggaca aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt
    11341 cctttggctg ttgcagggtt gttcttaaca atgatcgtac gtacaatcgc tgttccaatc
    11401 gttcatttga tggatgcggc tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac
    11461 atcattgctg tatgtttaca aggggttcgt atcgcggtcc cagcagcagc gttgctattt
    11521 atcccagcac aaactgtgca atcattcctt gaatcaatgc cagcatggtt aacagatggt
    11581 atggctatcg gtgggggaat ggtcgtagct gtaggttacg ctttggtaat caacatgatg
    11641 gctacaaaag aagtatggcc attcttcgtt attggtttcg ttgtagctgc aatttctcaa
    11701 ttaacattga ttgctctagg tgctttaggt gttgcattag ctcttattta cttgaacctt
    11761 tctaaaatgg gtggttcatc aaataatgga ggcggaagca actctggtga ccctttaggc
    11821 gacattttga atgactatta atcttgaagg aggaaaataa aatggcagaa gaaaaaatca
    11881 aattatcaaa aaaagatcgt ttagctgttg cgtggcgctc taccttcatt caaggttcat
    11941 ggaactacga acgtatgcaa aacggtggct gggcattttc tatgattcca gcaatcaaaa
    12001 aattatacaa aacaaaagaa gaacgttctg cagcattaaa acgtcacttg gaattcttta
    12061 atacacaccc atacattgct tctccaattt taggggttac attggctctg gaagaagaac
    12121 gtgcgaatgg cgcgcctgtt gatgatgtag cgattcaagg ggttaaagta ggtatgatgg
    12181 gtcctttagc tggtgttggt gacccagtgt tctggtttac agtacgtcca atgctaggtg
    12241 cactaggtgc ttcattagct atgggaggaa atatccttgg accaatcatc ttcttcttag
    12301 gttggaacat tattcgttgg gcattcatgt ggtatacaca agaatttggc tacaaagctg
    12361 gttctaaaat tactgatgac ctttctggcg gattgttaca agacgtaaca aaaggtgctt
    12421 caattttagg gatgttcgta ttagctgccc ttgtacaaag atgggtatca atcaaattct
    12481 taccagttgt atcaactgta aaattagata aaggtgctta catcgaatgg gataaattgc
    12541 cttcaggtgg tgaaggaatc aagagtgcat ttgaacaagt taataatggc ttagcccttt
    12601 ctcctgaaaa ggtaacaaca ttacaagaca acttagacca attgattcct ggtttagctg
    12661 cattaggatt aacattgtta tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta
    12721 tcatccttgg tttattcgta gtcggtgttg ttggacacgt catcggactt ttataatcca
    12781 taaaaatata gctcgggcgt tgtcccgagc tttttttata tagtatactg ttcataaaca
    12841 aatatctgga gggagaaaat ggttcaatca atcaatacaa aagtagactt ggtaattgat
    12901 gctacttcgt ttacgggtct gacagattac gggcaaatca tgataggtga taatggtttt
    12961 gagttttacc atgcgcgaga tagacgcaaa tatatccaaa ttccatggga agaagtggat
    13021 tatgtactag catcagtcat gctaaaggga aaatggattc ctcgatatgc cattcaaacg
    13081 aaaaaaaatg gtacatacac attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc
    13141 agagaatatg tagatgaaga tagaatggta caatcattga gtttctttga tgtagtgaaa
    13201 cgtggtattc gtagtatgtt taaacgcaag taaaaaataa agaaaacttc taagaggagg
    13261 ttttctttat ttttttactt tgcagaagaa aatatgaaat agcaaaacca attatttgtc
    13321 ccctttaaat cccatatgct aaaattgatt agcaaagaag gaggttatat tatgagttgg
    13381 ttatggacat tgattgttgg agccgtgatc ggagcaattg caggtgcgat caccagtaaa
    13441 ggaaattcga tgggctgtgt aacgaatatc attgctggac tagtcggttc aagtattgga
    13501 caagcgattt tcggcgaatg gggaccaaaa gctgcaggta tggcacttat tccatctatt
    13561 ttaggcgcag tgattttggt agcagttgtc tcatttttct ttggaaaacg atcataagtt
    13621 tttacaaaat agtaaactaa aaggagttgc agcgagactg tgactccttt ttggctttat
    13681 ttatttaaaa taaaaagaag ttttccggaa atttttattt aactgtcgtg tacgaagaaa
    13741 agtttgctat aatggaaaaa agcgtgcaaa gaaagaggga gtttcattat gatgctacgt
    13801 gtagaaaaat tgcgcaaaaa aatgcaagaa gaaaatttgg attcattttt ggttaccagt
    13861 ccgtacaatc ttcgttactt aacgaacttt acaggtacga caggattagc tgttattact
    13921 ttggaaaaag cattttttat tactgatttt cgttataccg agcaggcggc agcgcaggct
    13981 caagggtttg aaattattaa aaatgtcgga cctatttttg atgaggtagc aaacttagta
    14041 cgcaaagaag aactgcactc attagctttt gaggaaacaa cagtctcatt tttagaatat
    14101 tcggttttag aagaaatcat cgatgcggaa ttagtacctg tttcacagat gatcgaagaa
    14161 ctcagagaag tgaaagatga agaagaaatc gctattatcg aaaaagcatg tcatattgcg
    14221 gatatggcat atgatcacat cctgaaaatg atccaaccgg gcatgactga gattgaagtc
    14281 gcaaatcaac tagactttta tatgcgttct cttggcgctt caggtgtttc ttttgatacg
    14341 atcgttgcta gcggattgcg ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc
    14401 gaacaaggtg acttgatcac attggacttt ggttgctatt acgaagggta cgtttccgat
    14461 atgacacgta cattcgctat tggtgatcct ggggaaaaat taaaagaaat ttatcaaata
    14521 gtcttagatg ctcaattagc agttattgat gctgcaaaac caggaatgac tggtgtccaa
    14581 ttagatgcag ttgctagaga ttatatttca aaacatggtt acggggaagc atttggacat
    14641 tctacaggac atgggatcgg tttagagatc cacgaaggac caaacgtttc ttctcgtgca
    14701 gaaaagcaat ttgtaccagg aaatgtgatc actgatgaac cagggatcta cttaccagga
    14761 atcggtggag tacgtattga ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg
    14821 actcgttcac caaaagaatt gatcatctta tagtttcatc aatagaagaa gtaagcgtaa
    14881 aaatcagtgt tcacagactt tgttatagaa aatcactcga tttaatgata cactatagaa
    14941 gaacttttaa gctgtaagag ataataaagt tgggtgtttt ctccaattca tagcagtttg
    15001 cagctgatcg gcggcagttt tcttaccacg tgatcactat gccgcatcat agagaattcg
    15061 gatgtatctc gtgcttaggc aaagggagga ataaataaat ggcagatgaa aaaaatttga
    15121 ttttaaacgc aaaccaagat ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa
    15181 ttatcgggat tgcagcttct aaagtagaag gcgtatacgg aatgcgcgga acttttgcca
    15241 gcaatgtcac tgaattatta ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg
    15301 aggaagatgg cttgaaagta gatctttact gttatctaga atatggtgtt tcagttccta
    15361 aagtagcaat ggaaatgcag gaacgggtca gccagcaagt ccttttcatg acggatatcg
    15421 atttatctga agtcaatatc catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa
    15481 caatcgaaga actttttgaa gacgaagagg aagagaatga ataaagaatt atcaagacac
    15541 gagattcggg aaatggcatt gcaggcttta tttccattag acttcaatgc agacctaaca
    15601 aaagaagatg cgattttcaa cgcaatcgaa ttagaccacc gcgacatgat caacgaagat
    15661 gaatcagagt ttgttcctgt ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa
    15721 gatgaattgg ataaagtgat tgaaaaacat ttaaaaaaca attggcacat caatcggatc
    15781 tcaaaaatgg atttggtgat cttgcgtatc gcgatttttg aaatgactta tgtgacagat
    15841 gtaccagcgg ctgttgcttt aaatgaagca atcgagttgg caaaaacatt tagtgatgac
    15901 cgttcacgta aattcgtcaa cggtgtttta tccaatgtat tgaaagaatt agggctcttt
    15961 gtcaaatgat gttggtaaag agaaatct
//
LOCUS       DAFBQH010000064        15895 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.066, whole genome shotgun sequence.
ACCESSION   DAFBQH010000064 DAFBQH010000000
VERSION     DAFBQH010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.066"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(390..1337)
                     /locus_tag="LK694_002092"
     CDS             complement(390..1337)
                     /locus_tag="LK694_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBK5052936.1"
                     /translation="MHLEFSDYESLLVKICEDYYLEDINISEISKKYGITRYKALKYI
                     EDAREKQLVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADY
                     IQELIKKANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQK
                     AAERYESSYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDS
                     VEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGI
                     CKSKFKAESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
     gene            1525..2712
                     /locus_tag="LK694_002093"
     CDS             1525..2712
                     /locus_tag="LK694_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBK5052937.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            2693..3997
                     /locus_tag="LK694_002094"
     CDS             2693..3997
                     /locus_tag="LK694_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBK5052938.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            4023..5036
                     /gene="citC"
                     /locus_tag="LK694_002095"
     CDS             4023..5036
                     /gene="citC"
                     /locus_tag="LK694_002095"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBK5052939.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            5040..5333
                     /gene="citD"
                     /locus_tag="LK694_002096"
     CDS             5040..5333
                     /gene="citD"
                     /locus_tag="LK694_002096"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBK5052940.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            5338..6243
                     /gene="citE"
                     /locus_tag="LK694_002097"
     CDS             5338..6243
                     /gene="citE"
                     /locus_tag="LK694_002097"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBK5052941.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            6236..7795
                     /gene="citF"
                     /locus_tag="LK694_002098"
     CDS             6236..7795
                     /gene="citF"
                     /locus_tag="LK694_002098"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBK5052942.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            7792..8349
                     /gene="citX"
                     /locus_tag="LK694_002099"
     CDS             7792..8349
                     /gene="citX"
                     /locus_tag="LK694_002099"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBK5052943.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            8342..9205
                     /gene="citG"
                     /locus_tag="LK694_002100"
     CDS             8342..9205
                     /gene="citG"
                     /locus_tag="LK694_002100"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBK5052944.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            9439..9848
                     /gene="tnpA"
                     /locus_tag="LK694_002101"
                     /pseudo
     CDS             9439..9848
                     /gene="tnpA"
                     /locus_tag="LK694_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(10485..11657)
                     /locus_tag="LK694_002102"
     CDS             complement(10485..11657)
                     /locus_tag="LK694_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBK5052945.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRIGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LIASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWITFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYIKFAK"
     gene            12331..13143
                     /locus_tag="LK694_002103"
     CDS             12331..13143
                     /locus_tag="LK694_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052946.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
     gene            14778..15332
                     /locus_tag="LK694_002104"
     CDS             14778..15332
                     /locus_tag="LK694_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK5052947.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            15586..>15895
                     /locus_tag="LK694_002105"
     CDS             15586..>15895
                     /locus_tag="LK694_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052948.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     L"
BASE COUNT         5565 a         2356 c         2950 g         5024 t
ORIGIN      
        1 cgtggagaaa taataagaac taccagtcag ataaaacaag ataccgaaga ctcactgcgc
       61 aaagggtggg gaatgaataa taaagttaga tagaaccccc tttttatagc ataaaaaaga
      121 tttagaagga tatatgatcc tattgcatga gaagtacaat aaaattaata agaagtaaca
      181 ttaacgaaaa aacgtggaaa ttattcagtg aatacaaaga aattaaatct tgaaaagtaa
      241 tggagtatgg cttggaaaag ttcaaaaaat atcaagacaa gcttctcaaa aaaagggatt
      301 ttgatatcca ataaaaagag tgaatcattg tactgccctc caaaaaagta gtaccaaaaa
      361 tctaaatttt ttggaggatt ttacatgact cactcttttt ttatcaaaga aagtattttc
      421 caagcagttt tttcatccaa aaataaatct gtaaaaaaat taccattcaa ggcgcctaaa
      481 caactctccg ctttgaactt acttttacaa ataccaattc gttttggaat atcgaaaatt
      541 tcttttaaag aaagaccaaa agtcttatcc tcagatgctt tgatgaattc accattgata
      601 ttgaagggtc ttccgtaaag aaatccaaca gtatccgtca tgcttggaaa taggatttta
      661 cgatgcttgt tccagggttc gacagaatca atagaggata aggtgccaat tccagaaatt
      721 aacatatctg catgcgaagc ggttgctaag gtattagcta aagaaggttc tagagccatt
      781 agttcacgtg cagtttcatt gatgacatat aaaggaatac tgagagtcaa ataagaagac
      841 tcataacgtt cagcagcttt ttgaactaat cgtgtggagc cagctaagga attatatttt
      901 ccgatttcgc caatgaattg agtaaaaatt aattcttcac gagcacccat tttaaactga
      961 tcaatcactt tgtaaacggt atctccccaa gatagtgcaa ccacattggc tttcttaatt
     1021 aattcttgaa tgtaatcggc agcaaatttt gaaaataata aatcgtgttg tgataagtct
     1081 tcactacttt ttaatatata gatattcgtt ttaaagtaat ttttgaagat ttcttctagc
     1141 tcataattcc tagcatacgg agaattaatt gaaatactaa ctaattgttt ttcacgtgca
     1201 tcttcaatat attttaaagc tttatatctg gtgatcccat attttttaga aatttcacta
     1261 atattgatat cctcgagata atagtcttca caaattttta caaggagtga ttcatagtca
     1321 gagaattcca gatgcatagt atagctccta actttttcaa atagatattt tttctcatta
     1381 taccaaaaaa attgaattta gtcttttttt aaatttttaa aaataagcat cattttttta
     1441 aaatttaaaa tgaaagggtt tttttatttt aaaaaaagtg ttaaagtggt tttgtaatta
     1501 aaaaatgtaa aaaggagtga aggtatgctt gatgaagttt tagaaatcca taaaaaaaat
     1561 gtaggtgttt tacggatgga aacaacagaa gccgtgcaag atgatcaaga tttagcaaaa
     1621 atgtacaccc caggtgtagc agaactaagt ttaatgattg agaaaaatca tgctttggca
     1681 agagaattaa caattagtgg taaattagtg gccgttatta cagacgggtc ggcggtatta
     1741 ggtcttggaa atgtaggaaa ccaagctgga ttaccaatcg tcgaagggaa agcgctgctt
     1801 tataaaaatt tagcaggtgt caatgcgatt ccattagcca tcgaacaaaa atcagtagac
     1861 gagattgtac aaacaattgt caatttgcaa aatagttttg cagggatcca tttagaagat
     1921 attgcagcac cgaagtgttt tgagattgaa gaaaaattac aagaaaaact ttctattcct
     1981 gtgtatcatg acgatcaaga aggaacagcg atagttgttt tagctggatt gatcaatgct
     2041 gctaagatcc aaaggaaaac attaactgaa cttcgagttt tgatcaacgg catgggagca
     2101 tctggggttg caacagctag actattaatc gctgctggta tcaaaaatct aacattggtg
     2161 gataaacaag ggagattaaa gaagcaagat agtacattaa ataaatatca aagagagttg
     2221 ttggatcata tacatgatac aaataataat gcaagcgttt tggaagatgc aatcgttgga
     2281 caagatgttt ttatcggtct ttcaatagga aatgttttaa cgcctgaaat gattggaaaa
     2341 atgaatgata atccgattat ttttgcttta gccaatccag taccagagat cacacccgat
     2401 ttagcaaaag aatctggtgc aaagctaatc gctacaggtt cttcgaaata tcctaaccaa
     2461 gtaaacaata ttttagtatt tcccggattg ttcaagggac tattactatc gaaagcgaat
     2521 aaagtcgatc tttcattaca acttgaagta gccaaacaat tagctgctaa ggtcgcagag
     2581 cctacggtag aaaaattcat ccctaatgtt tttgatgaag gagtcgcaaa cgtcgtttca
     2641 caggcagttt tggattattt acaaaaacta aaagaaaacg aggataatat acatgctgaa
     2701 aacattaaat agaattaaaa ttggttttat tcctttatct ctttatatca ttttggtact
     2761 cgcgtatctt gcaatgacga taagcaatat cgttccagaa aatgatatgt tagtttcttt
     2821 gggtattatg actgtttttg cttatttatt agaagaaatt ggtaaaacta ttcctatttt
     2881 aaaagattta ggtggtaaag tattagttgt tacatttttg cctgcatttc tagtctataa
     2941 acattggcta cctcaaagta ctgtaactgt ggtttctgat tttatgaata ataacaattt
     3001 cttgatgttc tttatcacac tactagttgt cggcagtatt gcttcaatga atcggcaaac
     3061 attggttaaa gcaacttcac gtattatcat tattttaatt ttatgcgatg tgattggttc
     3121 tttgatagga acaggtgtgg gaatactcct tgggctatct gcttggaatg cgtatttctt
     3181 tatcgttgcg ccgattatgg ctggtggtgt tggggaagga gcgatgccat tatcgattgg
     3241 ttatgcagca atattatctg gtatgggaca aaatgatatc tttgggaaga ttctaccgtg
     3301 tgtgatgttg ggaagtttag tcgcagttct actgtctggt atgctcaaaa aaattggaga
     3361 gaaataccca caattaaatg gtcagggtaa tttgatcgat ggggatgatg aacatttaga
     3421 gaacttgaag ggttcagcta aaccagtcaa tattgaaaaa acattgttag caggcgtggt
     3481 agcaataacg atttattttg ttgctgtcta tgttgagcac ttattaaaat cactccttgg
     3541 cttttctatt ccttcacctg tgatcttatt agtctctctt atgggggcta aaatgcttgg
     3601 ttttatccct aaaaatatcg aagaaggtgg aaattcgttg tatggattca ctgttcgtgg
     3661 gatcacacct cccttacttt ttggcgtagg ggttgcaaaa acagattggt tagagctgat
     3721 cgaagtattt aaaaatcctg caacgatagt aacaatcatt gtgacggtag tagctattgt
     3781 cgctacagct tttatcgccg caaaattttt aaaaatgtac ccagttgata ctgccatcgc
     3841 agtttcatgt tgtagcggac aaggcggtac aggtgcttta gctatcttag ctgcgggtga
     3901 tcgcatggaa ttaatgccat ttgcacaagt tgctgttcga ctagggggtg ctatgaccgt
     3961 aacgttcgct atctttttaa tgggtctgct atcataagaa cataaagaaa ggacgtgacc
     4021 atgtgtttga tcaaacagat atccaacgat tatttttaaa aaataactat atcaaagacc
     4081 aatgggaaaa tcttttgctt gattcaggaa tacaaaagaa gcaaatcgaa gattttcaac
     4141 atttagatca aacattgggt atataccata aagaaaagtt agtaggaaca gtatcttatc
     4201 aaaacaacgt gattaaatat atcgccgtgt ctgaaaaata taaagatggt ggacatacat
     4261 ttaatgtact agtctctgca ataattcagg caatgagtca acaagagatt tttcattata
     4321 ttgttttcac gaaaccgcgc tatcaaaaaa gttttaactt tttaggtttt gaaacaatcg
     4381 tatctacccc aaaaatagct gtattagaaa tgggagatag ttctataaaa cagtacctag
     4441 agaggattcc taaagtgaat ggtgaaaaaa aagcagcaat tgtcatgaat gcgaacccat
     4501 ttacagaagg gcattattat ttagtaaaac aagctgccaa taacaacgat tgggtatatg
     4561 tatttgtact cgaaaaagaa caaaatcttt tttcgacgaa ggaccgactc aaattagttc
     4621 aacaaggatt acaaaaactg aaaaatgtca ttgttctatc aggtggcgag tatattatta
     4681 gccctgcaac gtttcccacc tattttttac gagaagatga tgaaatagca aaggagcaaa
     4741 tggtcgtaga tgctacttta ttcaaagaaa gaattgccac ttatttagat atctctgcta
     4801 gatatgtggg agaagaacca ctttccccaa tgactcgttc atataacgac atactggaaa
     4861 cgattttacc ccctgaaata aaagttcatg tccttgcaag gaagaaaacg gaacaacacc
     4921 aagttatttc tgcttctcaa gttcgtaaag cgtatcttgc tgggcaattg gaaaaaataa
     4981 aatatatggt tccagaaaca acctatcaat acttaaaaaa taaaagggag cgttaagtaa
     5041 tggaaataaa taaatcagcg attgctggaa cattagagtc aagtgatgtc caaatcatga
     5101 tttcaccgaa tacagaagga atagaaatcg atttagacag tcaagtgatt gagcaatttg
     5161 gaaaacaaat caaaaaagtt atctatgaca cactagcagt ttatcagatt aaaaacgtaa
     5221 aactcaaggt agtagataaa ggggcattag attgcgttat tgttgcacga ttaaaaacag
     5281 cgatccatcg ttccttaggt atccaacacc aagaaacacc gtgggaggtt taattagatg
     5341 atgaaagaac gtttgagaag aacaatgatg tttgttccag gaaataatcc agcgatgatc
     5401 aaagatgcag gtatttatgg tgctgactcg attatgttcg acctggaaga tgcagtgtct
     5461 atggcagaaa aagacagcgc aagaatgctt gtggctgagg cattgaaaac agtggattat
     5521 ggtgacacag aattaattgt tcgaataaat gacctgcact cagaatttgg acgtgcagac
     5581 atttttgcaa tggttaaagc cggcgcgcat gtattacgtt tacctaaaac agaaacctct
     5641 gaagatataa cagaagtaga aaaagtgatt atagaagcag aaaactatca taatgttccg
     5701 atagggcgaa cattgatgat ggcagccatt gaaggtgctt ctggtgtatt gaacgcacct
     5761 gcgatcgcca aagcatctga tcgattgatt ggaatcgcca ttggggcaga agactatgtg
     5821 acaaatatga aaaccaaacg atatccagat ggaaacgaat tgttttttgc tcgcagtatg
     5881 atcgttcatg ctgcacgtgc agcaggcatt tccgcatttg acactgttta ctctgatgtg
     5941 gatgatatgg aaggattttt aaacgaagta cgtttaatcc atcaattagg ttttgatgga
     6001 aaatcttgta tcaatccacg gcaaattcca gtagtcaata ctgtttatca gccgtcaatc
     6061 aaagaaatca aacgatcact agacatcatc tcggcaatcg aagaagcaaa agaaaaaggt
     6121 tctggcgtaa tttctttaaa tggaaaaatg atcgataaac caattgtaga acgtgcacaa
     6181 cggacattga tgttagcaaa agcatcacga ttgattgatg aggagggaaa tatccatgca
     6241 taataaacaa agacgtagca ttccatcaag tgttttaaca aagtataaaa aagaattcca
     6301 aagtgtttca ttatcgccac aaacgattca acgttgtgca ccaaaagtaa cgactgtaac
     6361 taatcagaca aataaattga tgcactcgat caaagaagct atagaaaaat cgggcttaaa
     6421 agatggaatg acgatttcat ttcatcatca tttgagagaa ggtgattttg tttttaacca
     6481 agtgatggcc gtgatccgtg aaatgggcat caaagaattg accttagcac caagttcttt
     6541 aacgaacact atgaatgaga tggttattga atgcatcaag gacggtacag taactcatat
     6601 cacaagcagt gggatgcgcg gcagcttagg tgaatttatt tctcatggag gaatggacaa
     6661 tcctgtgatt ttacgttctc atggtaatcg tccacgtgca attgaaagtg gtgaattgaa
     6721 aatcgatgtt gcatttctag gagtaccaac atctgatgat tatggaaatg ctaatgcgac
     6781 tgttggaaaa tccgtttttg gttcattagg gtatgcaaaa gtagatgcac aatatgcaga
     6841 taaagtcatt cttctaactg atcatttagt tccttaccct aatacgccca ttagtattcc
     6901 gcaaacacaa gtcgactatg tcgtggaagt agaagcaatt ggtgatccga ataaaatcgg
     6961 tgctggtgct acacggttta ctaaaaaccc aaaagaatta aaaattgctg aaatggtcaa
     7021 agatgtcatt gttcactcac cttatttcaa agatggcttt tcttttcaaa caggaacggg
     7081 tggtgcagct ttagcagtaa cgcgttactt agctagtgaa atggaagaaa aacagatcaa
     7141 agcttctttt gcattaggtg gcatcactaa accaacggtt gatttattgg aaaaaggtct
     7201 tgttgaaaaa gtgttagatg tccaagactt tgacaaagga gcagctgaag gaatggcgcg
     7261 gcttgagaac caacaggaga tcgatgcttc cttttatgct gatccaagta ataaaggagc
     7321 ggttgttaac caattagatg tcgctatttt atctgcatta gaaattgaca caaacttcaa
     7381 tgtaaatgtt atgactggtt cagatggtgt gctaagaggt gcgattggtg gacatcaaga
     7441 tgcagcaacg gctaaactta ctatcatctc agcgccgtta gtacgtggcc gaatacctac
     7501 agtagtaaat gatgttacta cattgatcac accagggaaa agtatcgatg tattagtcac
     7561 agaagtaggg gttgctatca atccccaacg taaagatcta atagctattt ttgaaagaat
     7621 tccacaaatt cctgttttta caatagaaga attacagcaa aaagcagaaa aaatagtagg
     7681 catcccggaa cctttgcaat tcacagatcg gatagttgcc tatgtagaat accgagatgg
     7741 ttcaatttta gatgtcgttc gtcaggtgaa agaatttgaa gaagagagat catgatgttt
     7801 gatactggag aaattcaaga aatcgaggat attttgatcc ataaagaaca acgtgctaac
     7861 ttgcaaagca acctgctttc caagtattca aatagttcat tgatggtctt tacattgaat
     7921 attcctgggc cgatcaaaaa taatccaatg attgagcagg tatttcgtca gggacagcag
     7981 tcaattcagc aaatgttttt aaaaaaaaag gtggaaatga cgtacacaaa agaaatcaat
     8041 ttgccaagtg gattggactg gtacgtaata gtaacagaag cacctttgaa tctcaaaaaa
     8101 tggttggtca cgttagaaga gcaagacgca ttaggtagac tttttgattt agatgtcttg
     8161 ttcaaaaaag gagagcagat aacaattgtc agtcgccaag ctcttaatca tggggaacgt
     8221 ccttgtttta tttgtgggaa atcagcaaag gtatgtgcac gtaatcgtac acatactgtg
     8281 aaagaaatgc aaaagaaaat cgaacaaata gtagccgatt ccttaaaagg agcattgaaa
     8341 catgcatgat aatattcaaa aaatagcaca agaagcacaa ttagcgatgc tttatgaagt
     8401 tacatgtatc aataaaccag gattggttga tccagtagac cccgggtctc atcaagatat
     8461 ggacattttt acttttctac aaagtagtgt agttttgact ccctattttg aagaatttat
     8521 ccaaacagga ttggaccgac aacatcaacc aattgaagaa acctttatgg cgattcgaca
     8581 gattggttta gaggctgaaa ctgcgatgtt ctcagtgaca aatggtataa atacacataa
     8641 aggtgctatc ttttcattgg ggatttttct tgctatttgt ggccggttaa ctatttggaa
     8701 agttccttgt aaaaaaagtt tctttcaaaa agaaattcaa aaaatgacga aaaatctact
     8761 taacgatttt gggaagattg atcaaacaaa accaaaagca tggacttggg gagaatatct
     8821 atttgttaac catggattga cagggatacg aggggaagcc aaaagaggtt atccttgtgt
     8881 ttttgaccga ggattgcctt attatcaagc atatcaaggg tcgcaacaag ataagatgat
     8941 agacacttta ctttttctat caatggaagt agaagatacc aatttgatca aacgaagtaa
     9001 taatagacat gtttttgaag agtatcaatt acttattcgt ccttattttg aattaggggg
     9061 tgtaaaaacc atacaaggta aaaaatattt ggattaccta aatcagcaat ttaaaaagaa
     9121 aaattggagt attggtggaa gtgcagactt attgatcctt acaatctttt tagataaaat
     9181 catggacaaa ggatggttat gttgatacta taatacatta ttttgtatta gtaagctaat
     9241 aactaaaaaa tggttcaata caaatatttt ataaaaaatg gtagtgccta tcctttcaga
     9301 ttgtagacaa agaaaaattt ctttgtctac aatttttttg ttaatgactt cagtcagttt
     9361 cgtgcgcaaa gcgtgcgaaa caagaaaacc atgcgtttct acacgtggaa gaactagcat
     9421 atcgaaagga gttttttcat gtcaaatgac gataaaagtt tagcccatac tcgttggaat
     9481 tttaagtatc atattgtatt tacaccaaaa tatcgacgaa aagtaatcta taggcaatta
     9541 cgaaaagata ttggaaaaat acttaggaag ttgtgcaaaa taaaaaacgt ggaaattata
     9601 gaagcacatg caacgccaga tcatattcac atgcttgttc aagtaccatc aaaaataagt
     9661 atatccagtt ttatagttat ctgaaagatc gtagtgctgt gctaatccac gagcagcacg
     9721 taaatcacaa atatagatac ggaaataaat cattttggtc ggaggaatat tatgtgagta
     9781 cagtcgaatt gaatcaaaag acaattgaaa aatacatccg tgaacaagaa gctgatgatc
     9841 gcatttgaga tagtataagt aaaagagagt agatgttgca ataacaaaag ggtaaaaatg
     9901 gactacctcc agtaaaatac cgaaaaatag tttaactttt ggagataagt ccatttaagg
     9961 gtctatagaa tgacaatttt tttaggggat tttactatta ttacttcaag acccgcttgg
    10021 attcgcaaag tgagtgaact cttaaaacta cctgatagta ggttgtttta agagttatct
    10081 gaaagtaaaa attgcattta tatttttgat aaatacgtaa atttatatat acgtatttat
    10141 caaagtttta ggccaaagga atattgtgta aatacactag gattgaatga atagaccaga
    10201 agaaattgcg tccaagaaca aaagaagcat gatagtgctc tatataaatt gggcattcaa
    10261 gaatctgaaa tttaaaggta gaattttagt tagactttta gattttaaac acaaaaataa
    10321 ccatacaagc aacaagaagc aaagtgctat attataaatt gtccgtccgt ttacttacat
    10381 aagatcattg gggctatttt taaaagagaa taatagataa taaatgaaaa aaaagtcaag
    10441 tccacaatcc tgtgtaaaat actatacaat gctctcacta ttttttactt ggcaaattta
    10501 atatattttc ttgatgaaga aagccactca tcgtgttgat caataaggac tgctccaatt
    10561 aaacgattgg ctgaaacgca gtttggaaag atacgaataa ttttttctct tctgcgcact
    10621 tcttgattca gtcgttcaat tagattggta ctctttagtc gattgtggga atttccttgt
    10681 acggtatatt gaaaggcgtc ttcgaatcca tcatccaatg atgcgcaagc ttttgaatat
    10741 tttggttgat cgatataatc atgaatcaat cgatttttag cctcacgagc taagttaata
    10801 tctgtgaact taaaaattcc tttaacagct tctctgaaag attttgaatt ttttttagga
    10861 atggtggtaa agatatttct taggaagtga acttggcatc tttgccaact tgcgttggtg
    10921 aaggattttc taatggcaga gactaatcct ttgtgcgcat cagaaataac gagttctgta
    10981 ccttgtaaac cgcgttcttt taggtattca aaaaatgtta tccaggtctc ttcgctttcg
    11041 ccactttgaa tcatgaagcc gataatttca cggtcgccat ctttggttat tccaatcgct
    11101 atatgacagc tttttgagag tactcgattt tcttctcgta cttttatata gagtacatcg
    11161 gtcattaagt aaggataatt tttttctgat aataaacgat tctgccactc gttaaccatt
    11221 ggttctagct gttctgttaa gctagaaacg aaggacttag agacggattt accacaaagt
    11281 tcttccacaa tttttgatac tttacgagtt gaaacgcctg atacatacat ttccaacatt
    11341 gaagcaatga gggctttttc gtttcgttga taacgttcaa acactgtggg tgaaaaatgg
    11401 ccatcacgtg ttctgggtac ttttaattct agcgtgccta tacgtgtcgt aaagctgcgc
    11461 tcataatagc catttcgttg actttgtcgg ttttctgttc gttcatattc ttttgcttga
    11521 atatattctg ttcgttgatt ttccattagt tgattaaata ccgttgttaa aatattttta
    11581 gaaacgtcat cctttacaga atattcaata atgctttgaa tctcttcgct tttcagtgta
    11641 aaatgtactt gggtcatgta aaagtcctcc tgggtatgtt tttgtcgtta aaaacattgt
    11701 accgtaaaag gactgttaca tggccttttt acttttacac aattataagg actttatcga
    11761 aaaaaagcag ttatttgtta aaatcattga tattattaat aataagtatt ttttgaaagg
    11821 ggaaatataa aaggtttttg aaaataaata aaaaatagta ttacttgttc tattgctagt
    11881 tattgctagt ctttattctt ttttgttttt tagggatgta caaaagatct caaccacagg
    11941 agacattgct tttcactttt ctagaataaa atgcttgtcc tcagtatttt ctggtccaat
    12001 aaattttacc acatttaata gctatggcgg aggcgtgaat ttttttatcc atatctaaca
    12061 tctttccagc cgtaatattt tactggacaa caaacaattt gattctaagt tatatactta
    12121 ctatacgttg ggatactgaa tatctgtaca attctagtga gctatcaata tggacaaaag
    12181 aatagatgca gccttcttat tcagctgttt gtatagcttt tatggattga tatatattag
    12241 cgtactgcaa tttcagaatc aattgcgttg acgattgtac cagttgtctt gtattacaca
    12301 tatactcttc tttatgaaag aaaaaactgc gtgattcttt tagctgtcag tatgagtttg
    12361 ctcatctata cccatattct ctccaccttt atgagtgtga ttgttattgg cttcttgttt
    12421 atcggctctc ttttctctaa gcggaaagag agaaaagaaa tgtcgaatct gatatcaaat
    12481 atgataaaag ctgttggttg gaccattctt ttaacagctt acgtttggta tccgatgtta
    12541 caacaagtcc tacatcaaaa aattaatcgt ccttttcgca caaaacttca agaaagggca
    12601 ttaaacattt ctgatagttt aatcggtgca gtaaataatg atttagttac atttacaatg
    12661 ggaacaattg gtgtggtttc atttattctt ccacttttct ttaaaaaaaa atttgccaat
    12721 aaagaaaaga ttattaattt ttgtgcagtg accacttggt tcttatcaac gaatcttttt
    12781 ccatgggaat ttttacaaaa aacacctatt caaatcattc agtttccttg gagaatcttg
    12841 ggttttcaag tgctttttgg ttcattgatc ttaattatag tgtttttaaa gtggaaaaca
    12901 agcaacaaaa aaagtatgtg tagccttgta ggaatagtcc ttcttatttt tacagtgact
    12961 gtagcaacag aagcgaatta ttcgcagaaa attcaatcgt ataaaggtcg tttaattatg
    13021 accaaaaaag atgttacact ttatacaacc tcgagaactg gaggtcttta tgactatgca
    13081 ccattagatg cattgaaata taaagatcat ttaaagaaac atgaagtaaa agtctatgat
    13141 tgatgggaga atgcatctca taaagcacat aattctaaaa taatctatac agtaggagat
    13201 gcgaatgagc aaaatatcac gttacctgta tttaattact gggaacagtt atgaaagtaa
    13261 atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa ggtacgggaa
    13321 aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta tctttattaa
    13381 ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc tgcggttaaa
    13441 tgaaaataga ttagatagat attataaata tgttgattct attttcatag caatggagaa
    13501 aatacattag caatatataa aagaaggtat caagagtcac ggtgaattta ttccgaaaag
    13561 tgaaaagaac aaatataaaa tttaataaaa atatagaact aaataggaaa acgtagacgt
    13621 aattttctac gttttccttt tcatatatat taaatattac ggaaatttat ataaactaat
    13681 ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt taggtctatt
    13741 taactcatca gacattctat gctcattttc tccaaatttg tttcaactct ttcgtactta
    13801 attttgcgta tttgaaaatt cttcttttag agtactttta ttacgatact agtatgatat
    13861 atatctatag caaacagtta aaatcttcct aaaataagcg aatagcattc ctaaaaccta
    13921 taaaattttt agatgaagcg acaagtggac aattaataaa ctacttgaac atgaaattat
    13981 ggatcacctt tcaaatttag aaaatggaat tcttatactt attgcgcaac atttattgat
    14041 tgcaaaatta tgtgacaaaa agaactagta gaagaaggaa taatggcttg ataataaagt
    14101 actacctatt aaaggtagta ctttatttaa tttagtataa attaacatac tactatattt
    14161 taagtttcat attatataaa ttaataatat actaccattt tttggtagta taatttaaag
    14221 gtaataagtt attcttttat tactatgttt gggttttata gcaaggaaag tttaataaga
    14281 gtttggacct agcaaacaat attaatgagg tcattaaatc ttattatctt tagtgatata
    14341 acgaaatatc tacacagaca aaattatgat cttgcgtaaa gttataaaat attaataaaa
    14401 atattttatt aatgaaaagc ttggtagcac ggttattcat atgctaattt atttcacttt
    14461 atagtaaggg gagttcttag tatattgtcc aaatttaaat ttcatataat ttaattatgt
    14521 aaattagcta ttctaagaac ttctacggat aactgcccgt ctaaaattta attaagagct
    14581 tagaatcttc atataagtaa ggttaatttt tttgtacaaa atagtcggaa tattctgagt
    14641 tactgaaatg atataaaatc ataaactaaa ccccttgatt ttcttcactt ttaacagagt
    14701 atatatataa gttcatgatt atggtaaaat atatatataa tcatgaactc agaatatttt
    14761 taagaaggga aacaatagtg agctataatg tacaaccgtt aaggacaaat gaagagatac
    14821 aagattttca gttttggcta agaagaacaa gtaatccaga aagagatagt tttttatttt
    14881 tgtttggaat caataatggg ttacgtatgt cagatattat aggattaaaa gttggagaca
    14941 tacgaggtaa aagtaagcct attattgttg aacataaaac aggtaagcga aaaccgattt
    15001 ttatagataa tttgagagaa gagattttat tatatactga aggtaaagaa gaaaatgatt
    15061 ggttatttcc aagtcggcaa caaggtcgac atataacaag ggatcgtgtc tatcagatat
    15121 atgcagatat agcagaaaaa ttaggaagag atgatatagg aacacataca ttaagaaaaa
    15181 cttttgggta tcactactac aagaaaacta gagacattgc taccttaatg tttattttta
    15241 atcatagctc gcaagcaatt acaaaaaggt acattggtat aactgaagat gaaatagggg
    15301 catcgttacg tggttttaaa ttgggaggct aattgagata ttaggatata ttttttgtgt
    15361 ataatgaaaa cttagaaggt gagattaatg aagataactg aattttcgga agtatttaaa
    15421 acgtttagta acatttacaa tagaatacca gagatagaac aaaatgttgt agttgataat
    15481 gattattata tagagaatga ttttaataaa ccaagaaaat atgacaacct atacaatttt
    15541 atacacaata gaacaacaat aaataacaac agtaacactc gagttttgat taattcagca
    15601 tattttcata ttaaatattt aaagaaatat gagtatgaag aagttgtatt agattatggg
    15661 ctaaatagac agtcattttt aatttatgca ataaataacg gaaatttgcc aagcaataaa
    15721 aagaacataa ttattgaaac atatgcctat ctggaggggg gatctaaaaa actaattaag
    15781 tcatgtaata tagagtatca attatcagta ggagaaatta aatgtgtttt cgaagaagat
    15841 ttagaacttg acttaaaaaa aatttttgcc agcattcatg attatatagg tttga
//
LOCUS       DAFBQH010000065        15687 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.067, whole genome shotgun sequence.
ACCESSION   DAFBQH010000065 DAFBQH010000000
VERSION     DAFBQH010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.067"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..111
                     /locus_tag="LK694_002106"
     CDS             <1..111
                     /locus_tag="LK694_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBK5052949.1"
                     /translation="ISREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            124..510
                     /locus_tag="LK694_002107"
     CDS             124..510
                     /locus_tag="LK694_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052950.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            1061..1285
                     /locus_tag="LK694_002108"
     CDS             1061..1285
                     /locus_tag="LK694_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052951.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMIREAT
                     PVEIDYCKLVYCGCGRWKEDHVQKNISKYI"
     gene            1303..1731
                     /locus_tag="LK694_002109"
     CDS             1303..1731
                     /locus_tag="LK694_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052952.1"
                     /translation="MEKSDTKANRYKPTAAEKKLLEVLINPDNLGKPVQELCSLAKIG
                     RTKYYDAMSKDGFVDLVNETTMDLIKGKASDVLNATYKYALTEKGHQDRKMILTIAGI
                     YADKQELKHSGGVDIRKQYEKMSDEELEELVKRYEKINDS"
     gene            1709..3127
                     /gene="terL"
                     /locus_tag="LK694_002110"
     CDS             1709..3127
                     /gene="terL"
                     /locus_tag="LK694_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HBK5052953.1"
                     /translation="MKKSTTLNRVEYAVVQRELAIRKARKSFWLFCQLLYPEFYKDDR
                     LYLKDLCHTLQDFYEDKIDKKILVINMPPRHGKTFTARLFVLWMFGQNPKTKIITGSY
                     NQILSSLFAQQTRDGIMVENENVKAEYFKDIFPEVQIKYGDAAKGFWSLDGSEEKNYL
                     ATSPGGTSTGIGANYVIVDDIIKTAEEAYNERVLDSHWEWYNNTLAQRMERPRKQILI
                     MTRWSSNDLAGKMLTRRKNNVHQICYKAVQEDGSMLCNEIMTHDEYLDVVQEMNVDIA
                     EANYQQEPIDQKGRLYQKFLTYDTLPDNIIKIWNYTDTADKGADYFASPVFAETSDHD
                     AYIIDVMYTKEPMETTEIAHANMIIRNRVNKVRIEGNNGGRGFRRNSERITKERGYRG
                     AVFEDFHQSDNKLSRILANSAWVENHVYYPDDWATRWPDFYEAMVSYQREGKNAHDDA
                     PDAVTGIAETIIEADDFEFIAY"
     gene            3139..4674
                     /locus_tag="LK694_002111"
     CDS             3139..4674
                     /locus_tag="LK694_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK5052954.1"
                     /translation="MFYSGITLKKYKRLRTKYSTQIEEGLFDPNAFMEDMKPFFDDRE
                     RKYLAYTSEKNEIDSREKPKTKIIKVNNKIHAGMYSVVVDQAVNHFTGIPIKWDYDVS
                     EQKRTFIQRMRDKLLGNDISAPSTPEEFETLTRQLSTMRFGMLDPDTATFQGATGLAF
                     RLLEPVETNDGWELRASNIEPWKAEKYENGAVYIKEKYDPYQKKFYEEMKVVTPKSIF
                     TYARYTDYPGTVQGAFKTLGNVPNPLGVINLTEFKNNTNRYCDFEVAEEISDAIDRVL
                     SDQQNEIEQFKLAYMLITGADLSEDKAKKMMEQLGIINLKDPNAKAGYVTKDLQKDFN
                     EYHFDLLKKQFYTVCKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAEVMAAYLDFAEKVTIDPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMIDVESRQSYPDGEYGGGIVESNRNEVLDQTSRTGRFSKTQNGKSNAK
                     ETK"
     gene            4754..5632
                     /locus_tag="LK694_002112"
     CDS             4754..5632
                     /locus_tag="LK694_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBK5052955.1"
                     /translation="MMEFYSTSDQKKYREYVEKNYKSLKMYDAKYKEFIEEYFPSFDY
                     AKVNRLLQMRSDIFRILAESAIDADVNKYFSDRLEDILQRSYVSNANVFAQILNVDLP
                     NYLSKEELERYLNYPWSGKTFSRRLWGNISSLEQKLSNAITTSVASGEGVLVALRTMR
                     EDPEICDMFKREESKYNKAIENLVRTEYAKFAQDGIEKSYIETGVDEYNVLTAKDEKV
                     CSICGGKAKNNPYKLSEAIIGENRAPFHGRCRCTDVPNMPKLGKDIDEEYERLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            5634..5951
                     /locus_tag="LK694_002113"
     CDS             5634..5951
                     /locus_tag="LK694_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052956.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYENMGCIVVRTDYKEGK
                     SSN"
     gene            6078..6701
                     /locus_tag="LK694_002114"
     CDS             6078..6701
                     /locus_tag="LK694_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HBK5052957.1"
                     /translation="MIMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            6716..7606
                     /locus_tag="LK694_002115"
     CDS             6716..7606
                     /locus_tag="LK694_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HBK5052958.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            7629..7844
                     /locus_tag="LK694_002116"
     CDS             7629..7844
                     /locus_tag="LK694_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052959.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            7856..8197
                     /locus_tag="LK694_002117"
     CDS             7856..8197
                     /locus_tag="LK694_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052960.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            8197..8568
                     /locus_tag="LK694_002118"
     CDS             8197..8568
                     /locus_tag="LK694_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052961.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            8561..8956
                     /locus_tag="LK694_002119"
     CDS             8561..8956
                     /locus_tag="LK694_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBK5052962.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            8958..9335
                     /locus_tag="LK694_002120"
     CDS             8958..9335
                     /locus_tag="LK694_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052963.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            9336..9944
                     /locus_tag="LK694_002121"
     CDS             9336..9944
                     /locus_tag="LK694_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5052964.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            9944..10285
                     /locus_tag="LK694_002122"
     CDS             9944..10285
                     /locus_tag="LK694_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052965.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            10527..13316
                     /locus_tag="LK694_002123"
     CDS             10527..13316
                     /locus_tag="LK694_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBK5052966.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            13306..14046
                     /locus_tag="LK694_002124"
     CDS             13306..14046
                     /locus_tag="LK694_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5052967.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            14043..>15687
                     /locus_tag="LK694_002125"
     CDS             14043..>15687
                     /locus_tag="LK694_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK5052968.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPK"
BASE COUNT         5631 a         2186 c         3268 g         4602 t
ORIGIN      
        1 attagccgtg aagtgggtta ttcagaaaga agcatacaaa ggatgaagtc ggaagctcta
       61 atagagtttg cagaagcgta taaacacgga agaataattg cttataaata gggggaccat
      121 ataatgtgga aagattatgt atcgttgaaa gagttgaaaa aagatcttgt tttcaaaaga
      181 attgtagaat ggtcagagag tgaattaatt ttagaagatg gaaccaaaat ggaagttgta
      241 tgtagcgaat cagattgctg cgcatgggcc gaaggtgaat tcaaaaatgt aaagcttgat
      301 gcagtgatta cagatataaa gatttttgat aaaggtaacc gtctctataa tggtgacgga
      361 catacctctt atgctgaagt cgttgtttat cataatcgta atgagatcgc taaagcagaa
      421 tgcacagcga atgatgggaa tggcggctac tattatagtg tttgtgcttt aaaggtcaaa
      481 gacaaacttt gtatagtgac tgacgcataa aaaaatggcg gttttttggc ggaatgatgg
      541 cggtttttag ccatttatca gtgatattat ggtagtgtcg aaagattagt gataggtctg
      601 agacaaaata ataataaaag gaacatcgtt ttattattgt ttcacaatta agcttcgata
      661 gacagcaacg gaaatattaa gaataaggat gtgaatttta actccttcta aattgttctt
      721 attatctatc atccgttgct gtctattgtt acatacagat cgcttaggcg gtctttttat
      781 tttggccgaa aacctacatt ttcgatggcc gattattgga ggaggaatag catgttcaaa
      841 ctatctgaaa tcattaagaa gtccgacgtt gagaaattag agatgttaaa aaagaagttg
      901 aagaaatagc agtctcttcg tgaggctgtt ttattttgct cacaaaaata gaccactatc
      961 gggtaatagt ggtcaggaat taaatgaaaa agatgttagg gttgttagct aagtataaca
     1021 tcataacgct tacaaaacaa tgcaagaagg ataaattatt atgagaactt actggtatgt
     1081 gtcattaaac aataaatatc cgaagccaat gaaaggacag cataggcgtg tagtcatgtc
     1141 tgttcaaatg aaggcgaagt attcgattgt agaaatgatc agagaggcaa cgccagtaga
     1201 gattgattat tgcaagctag tctattgtgg gtgcggccgt tggaaagagg atcatgtaca
     1261 aaaaaatatc agcaagtaca tatagagagg cggtgattaa ggatggaaaa aagcgatacg
     1321 aaagcgaaca gatacaaacc tactgctgca gagaaaaaac tcttggaagt attgataaat
     1381 ccagataact taggcaaacc ggtgcaagag ttgtgttcac tggcgaaaat aggtaggacc
     1441 aaatattacg acgccatgag caaagatggc tttgttgatc tagttaacga aacaacaatg
     1501 gatctgatca aaggaaaagc ttctgatgtc cttaatgcca cttacaagta tgcattgact
     1561 gaaaaaggtc atcaggatag gaaaatgatt ctgactattg ctggaatcta tgcagacaaa
     1621 caagaactca aacattctgg cggcgtcgat atccgaaaac agtatgagaa aatgtccgat
     1681 gaagaattag aagagttggt gaaacggtat gaaaaaatca acgactctta atcgtgttga
     1741 gtatgccgtt gttcaaagag agctagccat tcgaaaagct aggaaaagtt tttggctatt
     1801 ctgccagctt ctttatccag agttttacaa agatgatcgg ttgtatttga aagatttatg
     1861 tcatacgcta caagattttt acgaagataa aatcgacaaa aaaattttgg ttattaacat
     1921 gccgcctcga catgggaaga cttttacagc gcggctattc gttttatgga tgtttggaca
     1981 aaatccaaag acgaaaatta ttaccggatc ttataaccaa atcctgtcat ctttatttgc
     2041 gcaacaaaca cgtgatggaa tcatggttga aaacgaaaat gttaaggctg aatactttaa
     2101 agatattttt ccagaggtgc aaatcaaata tggagatgct gcaaagggtt tctggtcttt
     2161 agatggttca gaagaaaaga attatttagc gacttctcct ggtggaacat ccactggtat
     2221 tggcgcgaat tatgtaattg ttgatgacat cattaaaacc gcagaagaag cctataacga
     2281 gcgtgtttta gattctcact gggaatggta taacaacacg ttggcccaac gaatggaacg
     2341 gccacgtaaa cagatattga ttatgacacg ttggtcaagt aatgatttag ccggaaaaat
     2401 gttgacgaga cgaaaaaata atgttcatca gatctgttat aaagcagtac aagaagacgg
     2461 ctcgatgttg tgcaacgaga ttatgaccca cgatgagtac ttagatgtgg tacaagaaat
     2521 gaatgtagat attgcagaag ccaactatca gcaagaacca attgaccaaa aaggcaggct
     2581 atatcagaag tttcttactt atgatacgtt gcctgataac attatcaaaa tatggaacta
     2641 caccgacacc gccgacaagg gtgccgatta ctttgcgtct cctgtttttg ctgagacgtc
     2701 tgatcatgat gcttatatta tcgatgtcat gtatacaaaa gagccgatgg aaacaactga
     2761 aatagcccac gctaatatga ttattcgcaa tcgagttaat aaggtacgga ttgaaggaaa
     2821 caatggcggg cgtggattca gaagaaattc agaaagaatt acaaaagagc gaggctatcg
     2881 aggtgcagta tttgaagact ttcatcaatc ggataataaa ctgtctagaa tactggcgaa
     2941 tagtgcttgg gtggaaaatc atgtttacta tccagatgat tgggcgacac gttggccgga
     3001 cttttatgag gctatggtta gttatcaacg tgaaggtaag aacgctcatg atgatgctcc
     3061 ggatgcagtt acaggaattg cagaaacgat tatagaagca gatgacttcg agtttattgc
     3121 ttattaggag gtgggtcttt gttttattct ggaatcacat tgaaaaaata caaacgttta
     3181 agaacgaaat attcaacgca aatcgaagaa ggattattcg atcctaacgc ttttatggaa
     3241 gatatgaaac cctttttcga tgatcgagaa agaaagtatc ttgcttatac tagcgagaaa
     3301 aacgagatcg acagtcgtga gaaaccaaaa acaaaaatca ttaaggtcaa taacaagatt
     3361 catgcgggaa tgtattcagt ggtcgttgat caagcagtta accatttcac aggaatacca
     3421 attaaatggg attatgatgt ttcggaacag aaaaggacat tcattcaacg aatgagagac
     3481 aaattactgg gtaatgatat cagcgcccct tctaccccag aagaatttga aacactcacg
     3541 agacaattaa gtactatgcg gtttggaatg ttagaccccg atactgcaac atttcaagga
     3601 gcgacagggc ttgctttcag attactggag cctgttgaga caaatgatgg ttgggaacta
     3661 agagcaagca acattgagcc gtggaaagct gaaaagtacg aaaacggcgc agtgtacatt
     3721 aaagagaaat acgatccata tcaaaagaag ttttatgaag agatgaaggt tgtcacgccg
     3781 aaatcaatat ttacttatgc tcgatatact gattaccctg gcacggtaca aggagcattt
     3841 aaaacgctgg gtaatgttcc taatccttta ggcgttatca atcttacgga gtttaaaaac
     3901 aatacaaacc gttactgtga ctttgaagtt gcagaggaga ttagtgatgc tattgatcgt
     3961 gttttatctg atcagcagaa cgagattgaa caattcaagt tagcttatat gctgatcact
     4021 ggggcagatt tgtccgagga taaagccaag aagatgatgg agcaactagg gatcatcaat
     4081 ctgaaagacc ctaatgcgaa agccggctac gtgacaaagg atctacaaaa ggatttcaac
     4141 gagtatcatt ttgatcttct taaaaagcaa ttctacacgg tgtgtaaagc aattgatttc
     4201 aatgacgaag tctttaagtc gaatagttct ggggaagccc gaaagtggca aatcattagt
     4261 ttagaagcta agacaaatac caaagaacag tactttcgag aaggattgaa agagtgtgcc
     4321 gaggttatgg ctgcttattt ggattttgcc gagaaagtta caattgatcc ggaaaagatc
     4381 attttcacct tctctcgttc gcttccaaca gatttaagct acctagctga ggcattgcct
     4441 aagttggcac catacgtttc taagaggacg attcaaagtc agatcccgtt cgtcacagat
     4501 gttgattatg aaaacgaaat gattgacgtc gaaagcagac aatcttaccc agacggcgag
     4561 tatggaggtg gcatcgttga gtcaaataga aacgaagtac tggaccaaac gtcgagaact
     4621 ggaagattta gcaagactca aaatggaaaa tcaaacgcta aagaaactaa gtagtgtctt
     4681 tcctgatgcg ttgaaagaaa ttcaagcgga gttattatcc caagctgatt tacacgatat
     4741 tacctatgct gagatgatgg aattttatag tacaagtgat cagaaaaaat atcgtgaata
     4801 cgttgaaaag aactacaaat cactgaagat gtatgatgca aaatacaaag agttcatcga
     4861 agaatacttt ccttctttcg actacgccaa agtgaatcgg ctgcttcaaa tgaggtcaga
     4921 tatttttcga attcttgcag agagtgcaat agatgcagat gtgaataaat atttttcgga
     4981 tcggttagaa gatatcttgc aacgttcgta tgtatcaaac gcaaacgtat ttgcccagat
     5041 tttgaacgta gatttgccga attacttatc aaaggaagaa cttgaacgtt acctgaacta
     5101 tccttggtca ggtaagacat tctcgcgtcg tttatggggc aatatttcat ctctagaaca
     5161 aaagctatct aacgccatta caactagtgt agcaagtggc gaaggagttc tcgtggcatt
     5221 acgaacaatg cgagaagacc cagaaatatg cgatatgttc aaaagggagg aatcgaagta
     5281 caacaaggcg atcgaaaatc ttgtgagaac agaatacgct aaattcgctc aagatggtat
     5341 tgagaaatct tatattgaaa ctggcgtaga cgaatacaat gtcttaactg caaaagatga
     5401 aaaggtatgt tcaatatgcg gtggcaaagc aaagaacaac ccatacaagt tgagtgaagc
     5461 gatcattggt gagaaccgtg cgccttttca tggccgatgc agatgtacag atgttcctaa
     5521 tatgccgaaa ttaggaaaag atattgacga agagtatgag cgtttatttg gcgatttatt
     5581 agatgagttt gcacatgatt cttttggaat taatttgaaa cggaggaagt agaatgaaag
     5641 atttttttga agcagtacta acaattaatg taaatgctga tattgcagaa gcctacaaaa
     5701 cagctattga gtctgagaac catcctaatg gcttgagaga ccattggaat ggcaattatg
     5761 cctacgtggt tattggcgat caaactgtta attatcaaga taatactcca gttgataaga
     5821 ataccgttaa tttaacgatt caattattat ctcattcatt accaaattta aaagaaacag
     5881 ttgattggta tgaaaatatg ggatgtattg ttgttagaac tgactacaaa gaaggaaagt
     5941 ctagtaatta ggctttttta ttttgtccga aatgacgtta aactagcgca atactgggct
     6001 tggttgaatg gtggggcgca actattaaaa ctcaaagcaa tgcggggcgt gaaaacgaat
     6061 cgtggggcga aaggagaatg attatgaaac acaaatcatt aatgccaatg aatttgcaat
     6121 actttgccga aggtgacgat caaaagtttt cttttgatga cttcaaatct tttgtggaat
     6181 ccaatgaaga agctcaaaaa tttattcagt cacaatctca aagtgttgcc gacaaacaat
     6241 tggaagcttg gaaacaaaat aacttagata aaattaaaca ggataccatc aaggaatatg
     6301 aggaatctaa gaaaaacaag tcacctgagc aaattcaact ggaaaaacta caagctgaat
     6361 ttgaagcaga aaaagcgttg cgtgtgacaa gtgataataa agcatttgtt gcagaacaaa
     6421 ttgctggatt agaactagat ggagagttaa aagagtctat ttctcaattt atgctaaata
     6481 atcttgttag ttcggataca gatttcacta agaatgctgt tgaaggtttt acaagtgttt
     6541 tgaatgcaat taatgagaaa catgcagatg cattaaaaga actacaaatg aagtctgcat
     6601 ttggtggaac tcaacaatcg aataaccagg ttcagcagaa caatgaaaca ttaacaaatc
     6661 cagaagaaca attaggacaa attcttcaac aatttaacta ggagagtgaa aaattatgaa
     6721 aaaaacatct ttaaataatt tagagtattt ggatatttca ccagcgatta atgctatgca
     6781 agtaccaaat acaccttttt taagctattt acttggtgct ggaaaaacag agccagcaaa
     6841 ctcgacagaa attaaatggc gtgaatatga tatcaacaac gatgattctt ctgaaaaact
     6901 tgagggcgga gaatatccag atgctgaatc aggtcgaact tggtttaaca actatactga
     6961 aatttttaga aaatcaacct ctgtatctgg tacattagat gctattaatg tgaatggagt
     7021 cggaaatgaa ttaactaatc aagtagcact acgtggtatg gaaatgaaaa ttgatttgaa
     7081 ccgaaaattg attactggtg taaaagctga tgaaaatggt tctaaaggtc gtcgaatgaa
     7141 tgggattttg aacttgatca attcagcaaa taaggcagaa acagccactg cgggtgcagt
     7201 aacaagaaaa gatatcgata ctttatttaa attgatgtat gaaaaaggtt atatgggaga
     7261 aaaactatgc ttgatttctc cagatatgca ggagttaatg actgatgagt tagatggaaa
     7321 atcaacaaaa attgttcagt tcggggaaag agtaactttt ggattgcaaa ttggaaatat
     7381 cgtgtctaat tacggtacag gtattgctct actagaacca tcattgccaa aaggaacaat
     7441 tgccgcaatc gatactaatt atgtgaaact acgtccatta cgtgaatgga gagcagaaga
     7501 acttgcaaaa acaacggatt ctagacgtat cggtcttgta ggtgaatact ctcttgaata
     7561 caatgcttca aactctgggg caattttaaa tttaaagtct gaataaaagg gagttagtac
     7621 tccctttttt gataggagga attacagtgg taaaaaaaga tgaaactaaa aaagatgaag
     7681 tcgtgaaata tagagtaggt aaaactaaaa attttgttgg gtttgttcat cctaaaactc
     7741 gtagatttat cacagcagat tcaaataacg aatttatcat ttctatagat gataaagaag
     7801 caattgcaat tttggaagat gcaattgatg ttaatgaaat ttaggaagtg atctgatgga
     7861 tgaatcgcta aaaacggaaa tcattgagtc tacaaaagaa gattttccag atttgagtga
     7921 agaacgcata actaatttat tagaaataat tttgctagaa attgaatcat acaacacttg
     7981 taaaaatgat atttcatggg aaaagttaaa aagcgtgatt agtgaagtgt tgtataaaat
     8041 aataaaaaat gaatcagaaa aaacagtatc ttcagttaga cgtggcgata caacgattag
     8101 ttatgcttca acaacaaatg atgttagtga gttacttcta ggctatggtg atttgatacg
     8161 aagagttatt ggttgtggag gattggagtt tttttaatga atgaagcaga tattttagaa
     8221 actacttatg aagatagttg tattattgaa agactgacgg acattgaaga ttctaataca
     8281 aatattacta ttcaagatta caaaaaagta tatgataatc ctatttcttg cgctctttca
     8341 caaggtcaaa tcgatggact agcagtcata gaagatggag agatggtaaa tgtttcaact
     8401 gacacatata aattatttgt tcatcctaag attaaactaa aaaaaggaga tcgaataaca
     8461 ataactcaaa aggccagtgg cttaattttt tctctatttg ctactaagcc tttttactat
     8521 cctagtcatt gtgaagtgaa tttgatagga agtgagaaaa atgggtaatc tcaaatatga
     8581 atcaaatgca gaaaagatca ttgagaattt taaaaatatg actgtaattg ctcaaaaaga
     8641 aggaatatcc ttcgttaacg attcaatgaa taaagtcgtt agtttagcta aaccattaac
     8701 tcctgtaaaa tcaggtaatt taaggcgagg atatcgtgta gtaaaagcta gaaaactatc
     8761 aagcggtcga attgttggag cagttataaa caatgaacat tattttaaat atgtagaaga
     8821 aggtcatagg actaaaaatg gtggctttgt caaagggaaa ttcatgttaa ctcgtgcaac
     8881 gaatcttgca aatatgtctt atattcctcg aagatttaaa caaatggcaa taaaaatcgt
     8941 taagaaagga aagtaacatg aaagatgaaa tcattgctgc aatcagtagc aaattaaaag
     9001 aaatctatcc tgatgggaca atatatcttg attcagttat gcagtcaact aaagattttt
     9061 atttcgtttt atcagtaatg gaatctggaa ctgaaaatgt aggaattgat attcaaaatg
     9121 tttctttctt aattgatatt gcattgattg ataataaacc taatagaaat ttaattaatg
     9181 aattagtctc acgttgtggg acttttttta atacgattac aatcgatgag caaatattat
     9241 ttccaaaagc ctatttacct gatgaagcag atggtgttca acatattcgt tttacattag
     9301 aatttccaca atatattgaa tggagtgaaa gataaatggg agaaaaaaga agtaaaaccg
     9361 gaattatttc tgtcgaaaaa cctacttggt ttcctttgga agatgagaca gggaatttcc
     9421 cggtatatgg tactgcgttg ccaatgggaa cagcagtaag tatcaaacca acagctaatt
     9481 atgaaacaac acaagattat ggggattcag tggttcaaga tcaatttaca gcttttggtg
     9541 gtgcagaagt tgaactggaa gcaaatggat atactcataa agttttatct acaattactg
     9601 gagggaaaat tgttaaaggt ggtgccttgc gttctggaga agatattgcg caagatggtg
     9661 cgtttgcata tagacgtaaa aaatcgaatg ggaaataccg ttatacggtc ttttataaag
     9721 gacagtttgc tctagattca gatgaaacgt caacgattga aggaagtaaa gtaagtttta
     9781 ctcatccaac ttggaagggt tcatttgtag atgtaccagg acttggatat atgtattcag
     9841 ttgatgaaga tgatgaaggc gtggataaag caatgatcgg aaattggttt acaaaagtag
     9901 caattccaat tgaagaagca gaactttcag gaggtacaga ataatgtcaa aatatcaaac
     9961 aacgattaaa ttaacgaaaa aagatgaaga aggtaaatat gagcaagtac aatttaaatc
    10021 tgctgaattt ttaccaggaa cagttgtaga agatgctgca ggagttatgg aagaaatgca
    10081 aacagcgact gataaacaat cagttaaaaa agctttaagt cgtgcttatt catttattgc
    10141 agatacttta tttgagggac aatttactgg tgaagattat tgcaaaggaa ttgatgctcg
    10201 tgagattgcc tcattgacag gaaaattatt gaagtctgtt actgcaggtt ttgatgaaac
    10261 ttatacagag acgaaaaaaa agtaagtgag gcgctcaagt caccttcatt taagtattcg
    10321 attacttacc gagaattaga tataaagacg caattactcg aagcaggttg gacgttacca
    10381 gaaattgaac gtactgactt ggatgagtta atgcgtcttt ttgcttttag agatgcggta
    10441 aaagagcatg aagatgttga gtactatgat aatttcactc aattttagga ggtgatactt
    10501 tgaataacga tgacctaatt ctgaagatga tactagatga atctggtttt actgccggta
    10561 tgaataatgc tgtcaaaaag ttgaactctt ttgatgaaac aatcgaaaga aaaagtagaa
    10621 atagtggcag ttcattaggt agtatttgga aaatatttgc tggaagtttt ctagctagcg
    10681 gagtaactag aatcgtagga gctggctttg atctaatcaa gggttccata agtggagcgg
    10741 ttgatcgagt agatacgatg aataacgctc tacgaaattt ccaaaatatg ggatttagta
    10801 attcagaaat tatgaagaat atcggaaaga atgggctttt atctcaaggt attcaaggac
    10861 ttcctactgc tttgaatgat gcgataagtc atgttcaact tcttgcttct tctacaggtg
    10921 atttaactcg ttcaactcaa atatttaaag ctttaaatga tggaattctt ggtttcggtg
    10981 gttcaactga tcaagttaat gaagctgtta ttcaattatc tcaaagtttc tcaaacggaa
    11041 aagtagacgc acaaacttgg aactcaatga tcaacgctca acttggacct actctttctg
    11101 ctattgctaa aaagatggga attacaatgg gagatctgaa agaaggtttg tctcaaggta
    11161 agatttctgt tgaagaattc caaaatcaat taatagaaat ggataccaaa ggtggcggag
    11221 gacttaaatc attaagtcaa atcgctaaag attctactaa gggaattaaa acctctatac
    11281 aaaatgcaaa aacagctgtt acacgtggtg ttggtgaggt aatagaagga ttaaataaag
    11341 cattagtaga ttcagattta ggtggattta agggaattat tgataaagtg ggcagttcaa
    11401 tggaatcgtt cttaaaagta attgctgcaa atattcctac agcagtatca tttttaagta
    11461 atttatttaa cgaaattcaa aaatttggtt ctgctttgaa attcatgatg ccctttcttg
    11521 ttcctgcagc taccgctttt ggagcgttca tgtttcaact taaaggtata ccagcaataa
    11581 taaaaagctt caataatttt aagaatgcca taattggcgt tggaaattca ttaaagatta
    11641 tgggggcaat agctgctgct aattcatttg ttttaattat tggtgcagtc gttggtgcaa
    11701 tagctgtttt cggttacttt atggcaacca atgaagagtt tagaaacaag gttatatcta
    11761 tttggaatga tgtgaaagat tccgtaattg gtgtattaaa aaatataaag gactggggaa
    11821 ttgatacttg gaattctgct aaagaaatgg catcaaatgc agttgagggt gtcaaagatg
    11881 cttggtcagg aacaaaggaa tggttttcta acacctggaa aggtattaag gatggggcca
    11941 caggtttatt tgataaaaca gtagagactt caagaaatgc agtcgatagt gtaaaaaatg
    12001 catggtctaa tatgaagaaa tggttttctg atacttggca aagtataaaa gattcagcca
    12061 gagaaaaatg gaatgaaatt aaaggttcta ttatgtcggt tgctggtcca ttgattacag
    12121 gtataaagaa tgcattttta catgttactt tttatctaga gaccttatgg aataatttag
    12181 ttgagattgg taaaaacgtc tttgagattt taaaaaatgt tatacttgcg ccggttttgt
    12241 ttattacctc catgatttct ggtggatggg aagaaacaaa aaataatatg attgctgttt
    12301 ggaataatat taaagaaagt gccataaata tttgggaatc tataaagaat atatttgtaa
    12361 gttattttac aaatatttat tttgctgcac tcaatatttg gacagggttc aagcttacat
    12421 tgataaatat ttggaatgaa gtggtaaatc aagctaaatc gatttggtta aacataaagt
    12481 atttttttat taatctttgg attgatataa aatattttgc aattcagaaa tggattgaat
    12541 tgaagttttc tattattcaa acttggatcg atttaaaata taatgccatt actacttgga
    12601 ataatattaa acagtttttc aaagatactt ggaaaaatat taaagatacg gcatacaata
    12661 cgtggatttc tataaaaaat accatgatta atacatggaa taacattaag gactctttct
    12721 ggaatattgt tactggaatt gttaattctg ctgaaaatgc atggacaaat ctaaaaaatg
    12781 gtgtttcaaa tgctattaat cgggtaaaag aaatctttaa ttcgttaagg gaagtcaatt
    12841 tatttgaaat tggcaagaac atcattgatg gacttataaa tggtgtaaaa gaaaagtgga
    12901 atgctttgaa agatactatc aaaggtattg caggtaatat caaaaattgg attaaaggag
    12961 ctttagatat tcattctcca tcaagatgga tgcgtgatat ggttggtaaa aatattgttc
    13021 aaggtattat cgttggtatt gataaagagc aaagtaagtt agatcaaacc atgacaaatt
    13081 tagtaaaaac accgtctgtt caacctgtag taaatggaat aagtcaccta ccaattacac
    13141 aaacaaagca aaatatatct gctagtgaaa caaaagaaat tcatttacat ttaaatgttt
    13201 atggagattt acctgattca atggttcgga agattaccac aaaaattaaa acagaattga
    13261 caagacaaat gaaacgagat gctggtgcag taggaggaac attatatgca acttaagaga
    13321 ggacaatttt ttattaatca acattattct tctgaattta atgtgtatat tcaaaacagg
    13381 cctgcttctg tttcagctag tcgagtaatt gaattgagag agcgagaagg aaatgattct
    13441 atcattattg ataaaggcta ttacagaaat gtgactagaa agattgagtg ttactataaa
    13501 gcaccttcaa ttgatgtggt gcaggagtgg gaagatcgaa tcactgaatg gttagatatg
    13561 tcgtcttata gtgattttat tctttactat gatgaacaat atatttatca agctgtagta
    13621 atagaaggac ctgaatttaa aggaacaaga aaaacaggaa atattgtccc ttttgaattt
    13681 acagtgagca ttcgaccatt taaggaaaat tacaatggta gatttactat tcgacaaaca
    13741 gaaacttttg aaatttataa cccagagaag tattcttcaa aaccgcttat taaattgagt
    13801 ggttctggag atgcttcttt ttatattaat aaggacaaat atgatttgaa atcgttggac
    13861 agagaattat ttatagattc taaattagaa gaggcttacc gaaaattaga tggtaattta
    13921 gaacatcagg atcaagtcac tttattttta gattttccat ttttatatcc tggaaaaaat
    13981 gaaattaaat ggacgaaaaa tattcattca tttgaaataa tgcctaggtg gtggagaaaa
    14041 gtatgaaacc aagaatatat agtcctactg aaacagattt ctcaacgaat ggcttaggaa
    14101 ttttaaagga ttgtacaaga tgtgaaatat atgaagtagc taatggaaaa tatgaattag
    14161 agttggatta tcctttagga actagatttg atgaatattt tgaaaatgac tatcaaataa
    14221 aagcaaagcc aaacgatcaa gaagagtatc atatcttttt tattgatgat aaagatatag
    14281 atactttttt aaatacagta actatttatg ctcagagtcg tacaaatcga cttggaagac
    14341 gggtagtcac tcttgcggaa gtagactcta aaactggtca agaagcgata tcaattattg
    14401 aaaccaaaat ggataaaaaa tctgacatac gactttattc tgatattacg gccgtttcta
    14461 gcacaatctt tgaagcgaga aatgttttaa attgtattgc tggtgaacaa ggatcattgc
    14521 ttcaatattg gggtggagaa attaaacgtg aaccatttaa gctttctttg ttaaagcgaa
    14581 gaggtcgcaa taatgttgga acgattcggt atggaaaaga tatgtctggt ttaaaggtca
    14641 aattagattg gacaggtata aaaacaagac ttattccata tgctgatcct caaagtgagg
    14701 taggtacgac taatcgaatt tatggttcgc cagtagatag tgcctatatt aataactatc
    14761 ctgatgtgta tacagagcat gttcagttta cagaagaaca aggagtaaaa gacattaaga
    14821 gcttaaataa aatagctaaa aattacttta aaacgatcaa tcctggctgt gataagccta
    14881 aagtttctat tacggttgaa tttgataagt tgactgatag tgaagaagcg aaagaatttg
    14941 cgaagattag aaattatggt ctgtttgata catttaaaat atatcataaa aaatataata
    15001 tttatcttga atcaaaggtt agtggactac aatacgattc tttatctgaa aaaactttga
    15061 aattagaagc aggagacatt caagttgctt tttatcagca acaagctgta actattcagg
    15121 ataaattaaa agattatgca acgaataatt atatgagtga ttttaatgat tatgtttctt
    15181 caatgattac aggtcaaggc agtgcaggag gatatgtaag tttatggcca aaagaaaaac
    15241 cttccaacat ctttattatg gataatccag atttagaaaa ggcaagagaa gtactaagaa
    15301 tgaataaaaa tgggattgct ttttctaaga aaggatggcg ggggcctttt acttcagctt
    15361 ggacattaga tagtatattt aatgccaatt ttatacaaac tggattaatc aaagcagata
    15421 tctttcaaaa ttcttttaac aagactggag atgtattgaa attagttaat ggactactcc
    15481 aaatttggaa caacaaaaag aaaataatgg agttaactaa aaaaggaatg gaattttgga
    15541 attctaaggg ttcaattgga acgattggaa caactgattc tgctggtaat ccttttcctg
    15601 gggcttctac tccaacccct attgaagata attctttagt tattcgtaca aatggagacg
    15661 gcaaatatat tttgatttct cctaaag
//
LOCUS       DAFBQH010000066        15513 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.068, whole genome shotgun sequence.
ACCESSION   DAFBQH010000066 DAFBQH010000000
VERSION     DAFBQH010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.068"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(330..632)
                     /locus_tag="LK694_002126"
     CDS             complement(330..632)
                     /locus_tag="LK694_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052969.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(852..1218)
                     /gene="ssrA"
                     /locus_tag="LK694_002127"
     tmRNA           complement(852..1218)
                     /gene="ssrA"
                     /locus_tag="LK694_002127"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1389..2162)
                     /locus_tag="LK694_002128"
     CDS             complement(1389..2162)
                     /locus_tag="LK694_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK5052970.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2306..2656
                     /locus_tag="LK694_002129"
     CDS             2306..2656
                     /locus_tag="LK694_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBK5052971.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2637..3353
                     /locus_tag="LK694_002130"
     CDS             2637..3353
                     /locus_tag="LK694_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5052972.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3353..4801
                     /locus_tag="LK694_002131"
     CDS             3353..4801
                     /locus_tag="LK694_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK5052973.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4871..5380)
                     /locus_tag="LK694_002132"
     CDS             complement(4871..5380)
                     /locus_tag="LK694_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052974.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5370..6095)
                     /locus_tag="LK694_002133"
     CDS             complement(5370..6095)
                     /locus_tag="LK694_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052975.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6318..8438)
                     /locus_tag="LK694_002134"
     CDS             complement(6318..8438)
                     /locus_tag="LK694_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBK5052976.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8668..9846
                     /locus_tag="LK694_002135"
     CDS             8668..9846
                     /locus_tag="LK694_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBK5052977.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9862..10620
                     /gene="aroD"
                     /locus_tag="LK694_002136"
     CDS             9862..10620
                     /gene="aroD"
                     /locus_tag="LK694_002136"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBK5052978.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10643..11128
                     /gene="ybaK"
                     /locus_tag="LK694_002137"
     CDS             10643..11128
                     /gene="ybaK"
                     /locus_tag="LK694_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBK5052979.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(11199..12452)
                     /locus_tag="LK694_002138"
     CDS             complement(11199..12452)
                     /locus_tag="LK694_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK5052980.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12569..13918
                     /gene="gdhA"
                     /locus_tag="LK694_002139"
     CDS             12569..13918
                     /gene="gdhA"
                     /locus_tag="LK694_002139"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBK5052981.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            14030..15376
                     /locus_tag="LK694_002140"
     CDS             14030..15376
                     /locus_tag="LK694_002140"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBK5052982.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
BASE COUNT         5126 a         2750 c         3124 g         4513 t
ORIGIN      
        1 aaagacattt gggttatatt gtgagataat aataaagaag agtttaaagc gttccccaaa
       61 aaccacttcc ccataagtgt gttacgcttt aaactctttt atatttgaag ccattaaaaa
      121 gcataccata taactgtaaa aaataatggg aaaaagactt ataattggag tggtagttaa
      181 ttagtgactt atttttgatt ttatagcact gatactataa aatatagata tcatcatatt
      241 acacaatctt aatactaact taaaaaatat cccttttata agtatggtga taaaatccgt
      301 tccgggctac cttttttagg tagcctactt taatctttat acctttctgg atcaacgaaa
      361 gtatacttca aatagtcata acgccgatga tcgctccgtg cgtctggcac atcagtcacg
      421 atatcaaaca aaaaatacac atctttcttc attctggttt ttgttgccgg aattttaaag
      481 tagttcttat tagagtaata gagattgatt aataagctat cttcgattgc taaaaagaaa
      541 acttctgaat cccacacctt ataaaaatct ttgacaaatc tattcgaagg atcaaattta
      601 aaccataatt gtgtttttcc ttccatcaac ataatgttca cctcaaaaag agtatacgaa
      661 ctaatgttct ttttgtaaag taggaatatc attgataata tggatggaac atatatatta
      721 aaaagtgttc tttttgaatg ctattttgaa tgccattttc attcatttaa cgaaaatctg
      781 cgaaaataaa aaatgctcaa acccttataa aataaggatt taagcatcta gcgaaatctg
      841 ctgaaatctc acaatggaga cggcgggagt cgaacccgcg tccaaacaca tcgccactta
      901 aaaatctacg ttcatagtca actcatttaa ggattcactt tacagcttgc cgagtgacag
      961 gcattctgta tcgctagtct gatgttctct ttttcaactt acagacggaa agttgaaacg
     1021 tatcccacta aagttaggac ccttactcga gcacatgggc gatgccgaga ggatctacgc
     1081 taactgtttt taggcagcta aagcgtaaga gttgttttcg tttttagcag ttatatttaa
     1141 ctgtaacgtt tttacgtaga cgtaacctac gaaacgcaat tcaagctcga ctgtgcctgt
     1201 cgaatccgta acgtccccgt tagctaacaa gggttacgtg tatttttcat acaaaattag
     1261 tatagcatat attgggataa attcaataaa aaagcgaatc atatcccatt atttatataa
     1321 taactgcccc tttcttaaga aaaaataagc gattttctta agaaaagagg tagcttcaaa
     1381 aaaagatctt aaatcagatc aaaatacttc aatgcgtgga caattccgcc ttctgtattt
     1441 ttctttgtga taaaggtaga aagttctttt aatccgtcat aggcgttgcc catagcgatt
     1501 ttgttgtcgc aagcttccaa caaggcaaaa tcattcggac catcgccaaa tccaaaagta
     1561 ggaacttttt ccagctgaag ttctttttgc agacgtttga cacctgcccc ttttgagatt
     1621 cctttcttca ctgtatcgat cgagtaagga ccattccgat aaaaagttag ctcaggaaaa
     1681 tgttcgtgat agtattcgtc acctgattga gataagatca gcaacatatt tactgtgttt
     1741 tctttaaagc ctaaaggatc aatttccgga acatctgaat gaatgaagtc gtaagcattg
     1801 atcattgttt ggctatgacc ggtacaccag attttctgat cgttatagta agaaagttca
     1861 tgaccgttca actgtacatg atcatacatt ttttgacatt cttctgaaga aaatgtttga
     1921 gaatagattt cttcgccgtc tactcgaatg aaagaaccat tcatggtgac cgcagaagta
     1981 atgcccgcat cttccatgat agagtggatt tctgcttctg tacgtcctgt agcgatgacg
     2041 gggagaacat tgttttcttt taatgcgttc atagcttttg ccacttcggg tgtgattttt
     2101 gatttcgcat ctagtaatgt tccatcaaga tcaaagaaag tgattgcacg ataatttgac
     2161 acaaataaaa ctcctttttt attggaataa gtttatctta acaaagaatg aacaaaagac
     2221 aaacctttcg tgaagaattc ctgaacatat tggttcagtc aacaatttag agtatactat
     2281 aagaagagaa tttaggaggg aaagaatgat ttttttcagt attggtatct tattcttaat
     2341 aattggcagt ctttttcgga ttctgccctc aaaaggaaat ctgccttttt atgggtatca
     2401 ttcgcctctt gccgcaaaaa cagacgctca ttggcggctg gctcaaaaga caagcggaaa
     2461 ctggtttttt cttatgggct tactgatggc attgatcggc tattatttaa agacatctgg
     2521 gcatacgaat tattttctga ttgaaatgct tctgctagta tttccaatta tgcctatttt
     2581 tattatgaca gaaaagaaat tacagaaata tgacctggaa actggaggga acgataatga
     2641 atattttaat gattgaagat aatcaatctg tttctgaaat gatgcagatg tttttcttaa
     2701 atgaaggttg ggaagcaact ttcaagtatg atggaaaaga agggctcgat gcctttttag
     2761 aaaacccaca acattgggat atgattacac tagacctgaa tttgcccaca ctggacggta
     2821 tgctagtagg aagagagatc cgcaaagttt caagtactgt gccgattatc atgctgacag
     2881 ctagagattc tgaaagcgat caggtgatcg gcttagagat gggggcagat gattatgtca
     2941 caaaaccttt cagtcctctg acgcttattg ctcgaatgaa agctttgcat cgccgtagtg
     3001 agttggcaga agcaagagaa gaagcagtag tttcagatga tcattttgat atcgagacta
     3061 gtcatttcaa gatgaatacg aaaacacgag aagcgtatct ggacaaccgg ccaattgaag
     3121 gactgacgcc taaagagttc gatctattgt ttactttagc aaaaaaacca cgtcaagtat
     3181 tctctcggga gcagttgctt gaattagtct gggattatca gtactttggt gatgaacgga
     3241 cagtggacgc ccatatcaag aaattacgac aaaaaatcga aaaggtcgga ccacaagtca
     3301 tccaaactgt ttggggagtc ggctacaaat tcgatgattc tggtgttgcc taatgcgtta
     3361 tttgtatcag caactcttag ctttctgcgg aatggtcgcg ttgatcttat tgatcgttgg
     3421 agtttctttt acacaactga caaaacaaac gattgaagaa aacaattacc agcaactgtt
     3481 tggatatgct gaatcagttg aaaagacaac gcaaacctat gccgatacgc tcccgcagtt
     3541 tagtcaagat caggcgtttc aaaatgcatt gctgctgaca gagcaagtac tgacagaaca
     3601 aaatgtgaac tttatattta tcgataaaaa cgaacgtgtc atttatccga ctaatacagc
     3661 tttactgaat tttaccttaa cagctgatca atggaaaaat ctgaggaatg gacgtcaaga
     3721 aaaattcaca tcgaataaaa atattttagg acaagcacag gcgacctctt acgcacttgt
     3781 gccatttaat ttgaatcatg aattctatgg agcacttgta gttagtcaac ctgcgcgaaa
     3841 tatcgataac agcgttcgtc caattacact gaacctgttc aaaggattta ttttttccag
     3901 tatcgttgca gttattgcca gctatgcttt tgcagcattc caagttaagc gtatcaatcg
     3961 gttaaggaat gcgacgaaag aagtgacgaa tggtaatttt gatgtacagc tccccgtaca
     4021 tgacaaagat gaatttgatg agttagcaga tgatttcaat aaaatgacga attcattgaa
     4081 agaatctcaa gcagaaattg aagaacaaga aaatcgtcga agacaattta tggcagatgc
     4141 ttctcacgag atgagaacac ctcttacgac aatcaacgga cttctggaag ggttagaata
     4201 caatgcaatc cctgaaaatc agcgagaaaa cgcgattaaa ttgatgaaaa atgaaacaga
     4261 gcgtctgatc cgtttggtga atgaaaactt ggattatgaa aaaatccgta cgaatcaaat
     4321 cagtatggtc attaaaaaat tcaatggaac agaaacattg aaaaatattg ttgcacagtt
     4381 agaagctaaa gcagaagcag caggagatac attgacattg aaagccgttg atgatattga
     4441 tgtttacgct gattatgacc gatttgtcca aatcatggtc aatattattc aaaacgcgat
     4501 acagtttact gaaaacggcc aaatcacgat tactttagaa aaaggctatc ttgaaacgat
     4561 catcacgatt gaagatacgg gaattggaat gtctgaacaa caaatgaaaa gtatctggga
     4621 tcgttattat aaagtagatc cttcaagaaa aaatacaaaa tatggtgaat caggattagg
     4681 tttaccaatc gtgcagcaac ttgtacgctt gcataaagga aaattagaag tagaaagcga
     4741 actaggaaaa ggaacaaaat tcgctgttcg tttgcctgac gtagaaatca aaaaagagta
     4801 aaaaacaagc ggtataaaag atcatgaatc gatcttctat accgcttgtt tttactataa
     4861 aatgattgat ttagcccgcg ggctctaaac gatcaatata aggactttga gcaatagtag
     4921 catcatgagt aaagagtaag ataccttgat tttcttcatg agctaaataa gaaagcaagt
     4981 ggagtagctc ttggatctcc aatacactaa gttcatcaaa tgtatcttca ataacaatat
     5041 tgtttcgctt ggaaagcaag gcatgaagta attgcagctt tactttttca aaagacgtca
     5101 actcagctgc cgcacaatta agcaaggaag gagagatatc aagtttttct tgataggcca
     5161 aacgatcttg tttgatttct ttttccggta catccaacag gacatggtct ttaagattca
     5221 gataaggaaa catcggccaa tttttttgga tgatcgcaaa attatccagt tttgcaacaa
     5281 gaacctcttt tttcaagtgc tccaatattt tatgctgttt ttcgagagga cacaaaataa
     5341 cggtcaagtg ttcagcatga atcaaatatt caggtttcat tacgtccagg cctcctactt
     5401 tgcaaatgat ggataaaaaa agtgaaaggg aatagactaa gaatgagaca gcatccactg
     5461 agcccaataa gagtttgggc aaatccctta agtatatctg tgaaaaaaag atcattttgg
     5521 aaatcgatgg taaaaaaagt ctgttgatcc atcggaattg taagtccgct taccgaagtc
     5581 aagatatcac ggatatctgg cagttcagta gtctttaaga caagaaaatt gatcctttga
     5641 agaagcgctt cataaactga ctggaagctt acgatccaca ataaaaccaa gatacaacaa
     5701 agaaagagag gaaagaacag ctgccagaaa ataaaaagga ataatttggt tttagaaaga
     5761 ccagtcaggt gccaattgat caattcctgc ttgttttgcc tgactgaata gtaaaagaaa
     5821 aagataaaaa ggaaggcaaa tagaaaaaat aaactgaata tgccttttat ataaaatcgg
     5881 ctagctaatt ctaaagccgg aagaagctct gtagatgcag aagcgccaat aaatgaaagc
     5941 cgatcttgga cttgattggc taaagccgat tgcaaatggt aaagagtcag gcatgctatc
     6001 aataaaaaag cagaaaagct tgaaaataaa caatagagta ttgtctgctt tttgtaataa
     6061 attaatcgag cagtagcata gcggattatt ttcatacttt tcacctctat aaaaagtata
     6121 gaaaatgaat atgaataaag tatgaaattc ctttagtgaa atcacgaatt aaaaccagaa
     6181 aatattttgt aaaaagcaag aggttgtgaa gaagctgccc tattctatta ccgtagatgc
     6241 aagcttttga acactgttta ttcgaaaaga ggagcagcga caataatttt gtcgctgctc
     6301 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
     6361 gtttgcgttt tataaagatc aacagttgat tgtccgttgt gttcactaaa gacactagtt
     6421 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
     6481 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
     6541 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
     6601 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
     6661 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
     6721 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
     6781 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
     6841 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
     6901 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
     6961 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
     7021 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
     7081 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
     7141 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
     7201 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     7261 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     7321 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     7381 ctaatgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     7441 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     7501 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     7561 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt catgctggag accattctca
     7621 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     7681 ttttttcctc tagcgagccc gaacaaatcg tcatttggtg ctgcataatg ttcttttaca
     7741 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     7801 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     7861 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     7921 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     7981 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     8041 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     8101 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     8161 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     8221 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     8281 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     8341 aaataggcaa aaatcgtttt caaccaaagt aaaacgacta gtaaagaaaa aaatccgagc
     8401 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     8461 cttaatcaca gaagtgtcct gaaaccatgt tacacagctc acttattctc atttatttta
     8521 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     8581 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     8641 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     8701 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     8761 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     8821 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     8881 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     8941 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     9001 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     9061 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcatagg aggctatgag
     9121 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     9181 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     9241 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     9301 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     9361 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     9421 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     9481 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     9541 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     9601 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     9661 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     9721 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     9781 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     9841 cattagaagg agcgatgcag aatggttcgg gtaaagggat tgaatatagg aagcggtaga
     9901 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     9961 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
    10021 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
    10081 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
    10141 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
    10201 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
    10261 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
    10321 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
    10381 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
    10441 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggttggc
    10501 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
    10561 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
    10621 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
    10681 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
    10741 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcgta tatttaaaac
    10801 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
    10861 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
    10921 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
    10981 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
    11041 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
    11101 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
    11161 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
    11221 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
    11281 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
    11341 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
    11401 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
    11461 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
    11521 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
    11581 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
    11641 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
    11701 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
    11761 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
    11821 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
    11881 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
    11941 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
    12001 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
    12061 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
    12121 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
    12181 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
    12241 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
    12301 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
    12361 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
    12421 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
    12481 tctgaagaaa gaaaacagaa aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
    12541 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
    12601 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
    12661 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
    12721 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
    12781 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
    12841 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
    12901 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
    12961 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
    13021 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
    13081 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
    13141 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
    13201 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
    13261 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
    13321 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
    13381 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
    13441 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
    13501 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
    13561 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
    13621 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
    13681 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgtccagcg
    13741 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
    13801 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
    13861 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
    13921 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
    13981 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
    14041 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
    14101 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
    14161 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
    14221 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
    14281 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
    14341 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
    14401 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
    14461 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
    14521 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
    14581 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
    14641 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
    14701 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
    14761 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
    14821 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
    14881 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
    14941 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
    15001 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
    15061 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
    15121 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
    15181 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
    15241 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
    15301 caagaaaaac atgttcgcac ttctaggcaa accaggcttt gaagatcttg ctaaagaatt
    15361 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
    15421 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
    15481 ttcagaataa ggggctgtga caagacgttt gtc
//
LOCUS       DAFBQH010000067        15447 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.069, whole genome shotgun sequence.
ACCESSION   DAFBQH010000067 DAFBQH010000000
VERSION     DAFBQH010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.069"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            226..894
                     /locus_tag="LK694_002141"
     CDS             226..894
                     /locus_tag="LK694_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBK5052983.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="LK694_002142"
     CDS             complement(1094..1480)
                     /locus_tag="LK694_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052984.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="LK694_002143"
     CDS             complement(1452..2834)
                     /locus_tag="LK694_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBK5052985.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="LK694_002144"
     CDS             complement(2990..4177)
                     /locus_tag="LK694_002144"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBK5052986.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="LK694_002145"
     CDS             complement(4184..5215)
                     /locus_tag="LK694_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBK5052987.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="LK694_002146"
     CDS             complement(5231..6685)
                     /locus_tag="LK694_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5052988.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="LK694_002147"
     CDS             6860..7765
                     /locus_tag="LK694_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBK5052989.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     KNFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="LK694_002148"
     CDS             7856..8533
                     /locus_tag="LK694_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBK5052990.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8530..8700
                     /locus_tag="LK694_002149"
     CDS             8530..8700
                     /locus_tag="LK694_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052991.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8814..9710
                     /locus_tag="LK694_002150"
     CDS             8814..9710
                     /locus_tag="LK694_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK5052992.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="LK694_002151"
     CDS             complement(9765..11135)
                     /locus_tag="LK694_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5052993.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="LK694_002152"
     CDS             11373..12119
                     /locus_tag="LK694_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBK5052994.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="LK694_002153"
     CDS             12116..12955
                     /locus_tag="LK694_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBK5052995.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="LK694_002154"
     CDS             12956..13906
                     /locus_tag="LK694_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBK5052996.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="LK694_002155"
     CDS             14047..14799
                     /locus_tag="LK694_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5052997.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..>15447
                     /gene="queG"
                     /locus_tag="LK694_002156"
     CDS             14818..>15447
                     /gene="queG"
                     /locus_tag="LK694_002156"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBK5052998.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRM"
BASE COUNT         4991 a         2911 c         3103 g         4442 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaat aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccggc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaatatcta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagtg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aatttctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctgcattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacaaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagtcggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatg
//
LOCUS       DAFBQH010000068        15347 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.070, whole genome shotgun sequence.
ACCESSION   DAFBQH010000068 DAFBQH010000000
VERSION     DAFBQH010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.070"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..563)
                     /locus_tag="LK694_002157"
     CDS             complement(<1..563)
                     /locus_tag="LK694_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HBK5052999.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="LK694_002158"
     CDS             complement(750..1400)
                     /locus_tag="LK694_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053000.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="LK694_002159"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="LK694_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBK5053001.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="LK694_002160"
     CDS             complement(2912..3910)
                     /locus_tag="LK694_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBK5053002.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="LK694_002161"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="LK694_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBK5053003.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="LK694_002162"
     CDS             complement(4926..5657)
                     /locus_tag="LK694_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBK5053004.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="LK694_002163"
     CDS             complement(5661..6926)
                     /locus_tag="LK694_002163"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBK5053005.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="LK694_002164"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="LK694_002164"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBK5053006.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="LK694_002165"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="LK694_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBK5053007.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="LK694_002166"
     CDS             12268..13770
                     /locus_tag="LK694_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBK5053008.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="LK694_002167"
     CDS             13772..14509
                     /locus_tag="LK694_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK5053009.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14554..14727)
                     /locus_tag="LK694_002168"
     CDS             complement(14554..14727)
                     /locus_tag="LK694_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053010.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14703..>15347
                     /locus_tag="LK694_002169"
     CDS             14703..>15347
                     /locus_tag="LK694_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5053011.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLA"
BASE COUNT         4519 a         3288 c         2590 g         4950 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg aattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc gctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaat aaaaaccaaa
    14521 aatgcacggt cgccatgaag ggaccgtgta tttttatctt cttattttct cacctaatcg
    14581 tatcagccgc atcgcttgtt ttcctgcaca aacaagcaat cgctctacct gttccatcgc
    14641 ataagaaagt accatcggtt cattgatgat atcaagtaca tagtcgatac caccctcact
    14701 taattgagct tgatttttct tctccatatt atgtagttgt gggacaactt tctgacaatc
    14761 agcaattgtg tcaagaacgg atcaatcctt tattggagtt gctcgaaagg gtcttctctt
    14821 attacggaca ggctttgctc actgtacata gacaacaaat cattatccta gtcaatcgga
    14881 tttccagagc ctcattgcta tcattgcttg ataaaatcca gacaaagttt aataaaatgt
    14941 atcagttaca gctaaacttt ggaattgggt cgctttgtta tacagagcaa gagacaccgc
    15001 aatctttcct tcatgccaaa caagtttgcg aatggatagc tttccatcaa tctgtgaacg
    15061 agatccgctt ttttgaagat ttagacctcg ggatcgtttt accagcaatc ccgtcagatc
    15121 aacgaacatt atatgtaaaa cgtattttga aaagcctgac agaagaagaa gtacatttgt
    15181 tcaagaagac tttggcatgc ttcagtaaaa ataacggaag catcaaaaat tgctcagaag
    15241 aattatttat tcataaaaac accttgcaat atcgattgaa caagttccat tctctcactg
    15301 gttatacccc tagaaactat gacgattatc atattctaaa gctggct
//
LOCUS       DAFBQH010000069        15316 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.071, whole genome shotgun sequence.
ACCESSION   DAFBQH010000069 DAFBQH010000000
VERSION     DAFBQH010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.071"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..343
                     /locus_tag="LK694_002170"
     CDS             <1..343
                     /locus_tag="LK694_002170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053012.1"
                     /translation="NIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQDK
                     KNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQKK
                     RDNNDQYLPKK"
     gene            424..1044
                     /locus_tag="LK694_002171"
     CDS             424..1044
                     /locus_tag="LK694_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053013.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     EIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQIS"
     gene            complement(1374..2600)
                     /locus_tag="LK694_002172"
     CDS             complement(1374..2600)
                     /locus_tag="LK694_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK5053014.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(2672..2830)
                     /locus_tag="LK694_002173"
     CDS             complement(2672..2830)
                     /locus_tag="LK694_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5053015.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            3518..4258
                     /locus_tag="LK694_002174"
     CDS             3518..4258
                     /locus_tag="LK694_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBK5053016.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGETNYIESSKN"
     gene            complement(4350..5537)
                     /locus_tag="LK694_002175"
     CDS             complement(4350..5537)
                     /locus_tag="LK694_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBK5053017.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            5859..6701
                     /locus_tag="LK694_002176"
     CDS             5859..6701
                     /locus_tag="LK694_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBK5053018.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            6701..7648
                     /locus_tag="LK694_002177"
     CDS             6701..7648
                     /locus_tag="LK694_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBK5053019.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            7663..7896
                     /locus_tag="LK694_002178"
     CDS             7663..7896
                     /locus_tag="LK694_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HBK5053020.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            8227..8811
                     /locus_tag="LK694_002179"
     CDS             8227..8811
                     /locus_tag="LK694_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK5053021.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            8942..9610
                     /locus_tag="LK694_002180"
     CDS             8942..9610
                     /locus_tag="LK694_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBK5053022.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            9621..11036
                     /locus_tag="LK694_002181"
     CDS             9621..11036
                     /locus_tag="LK694_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK5053023.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            11054..11623
                     /locus_tag="LK694_002182"
     CDS             11054..11623
                     /locus_tag="LK694_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HBK5053024.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            11733..13487
                     /locus_tag="LK694_002183"
     CDS             11733..13487
                     /locus_tag="LK694_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5053025.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            13447..15213
                     /locus_tag="LK694_002184"
     CDS             13447..15213
                     /locus_tag="LK694_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5053026.1"
                     /translation="MQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
BASE COUNT         5257 a         2159 c         2728 g         5172 t
ORIGIN      
        1 aaatataaaa actagtacga aattaatttg taaattattt aagcttgata aaataagcaa
       61 tatcgatgat gtaaagtcca agatattaac aagaaaagta agcgaaccag taaagctaac
      121 ggcacaagat aaaaaaaatg gaattgttct ttatggtgat gatgcaaagt ataactataa
      181 ttgggatacc tcctcagaag ctgaggtatt aagtattcct actaccgata cttcagggga
      241 aaaagtcacc tttaaaataa attttataac ggcaatttct aagctggagt atgggaagca
      301 aaaaaagaga gataacaatg atcaatatct acctaaaaaa taacgaatta atgatgcata
      361 tgttaaagtt gtttttttga attaaacaac tcaatttatt cagttatatg ttcatcgcag
      421 caagtgaaca ttaatcgagt tataaaatta ataggagcta aaaatataaa taaagttcga
      481 tcagaattta tttttccaga agattttttt aactggtttt tttatcaatt cagtgaatgc
      541 aagcgactct taggtgagaa ttattactta aaaggaatcg gcggatttat ggggaatgtg
      601 gctgatgaac aaaatattat aaaaggaata tctgaacaaa catcagagct tacagtaact
      661 aaagcattta taagtaatgg tgaaagtttg aaaaatgtaa ctgcgcgaat tagaaaagaa
      721 aatgaagtag aaattgtttt tggaattgac catgaatgta attcagtgat atatgtaaca
      781 caatcagcaa atttaattct ttttgagaat ggttgcaagg acgattttat ggtggtctat
      841 ttgtacgata tactaattcc aaaacttcgt tccttatata tgtcttctga aagtattttt
      901 gtgagtgaac aaaagggatc tttctcgaaa aaaataggta ttgaagtaat tgaatcgatt
      961 atgtgtcata atcatattac gctagaagaa ttagaaattt tgatagggca aaaaaataat
     1021 aacacgaaaa tacaaatttc ttagaaatca ttttttattg agatatctag ataaatgatt
     1081 tattcttcaa tcaaccaact cgccaagctg aggtaatgtt ttgaggtaat gataacatac
     1141 aactttttat accctagtaa aactctataa acaaagactt attaatttcg catgaaatac
     1201 attcctgtaa aatgcaataa aatagagttt tgtttaacca ggtctcaaaa aggctcgtaa
     1261 agcatctcaa ttctctaaac gttaatatta tttatatatc aatgtttcaa ggcacttttc
     1321 aattgtttga aaagtgtctt tttcttgttt aagaacacat ttaagaacac atactaatca
     1381 catttattca atcctctctg aaaggcttca aaaacagttt tatctactac atctggtgta
     1441 tttacgtata ttctcgttgt agaaatatct gagtgtgtta aagcactaga tatttgattc
     1501 atatttgctc cgccttgtct agctatagtt gcataggtat gacgaagctt atgtggagat
     1561 aaatgagcca attcaggatg tcgtctttta attgaattga ttcgatagtt taggtaatca
     1621 gcgtgaacag ggcaatttac ttctccggat ggtttagtat aggtaaagag atactgatct
     1681 ggtgtttgat taatttttaa tctcaacaat tgctcagctt gcactgattt ccatttgctt
     1741 agtaatgcca ttacaatttc gggtacttga attacggtat cctttcgacc ttttgtaaat
     1801 ttcttttttt gatatttatc caaagcatgt ttaaggcgaa ccgtttgttt ctcaaagtcg
     1861 atatatttcc attgcaaagc atacgtctca ctttttctat caccaagata tagggtaagc
     1921 atgaaaagag tatagtcatg aagagtaagc aagtgattat tcaagtcagt gtttactgct
     1981 tctatccaag caataagctc ttctgacgat aatgcttgat taccttcccg tatccttctt
     2041 tcttctaaag caattttttt aggtgcagtg atacattgaa taattttggt agttcggtta
     2101 aattcaatat aatttagtat ttctgcaata tcaaacatag aacggacata tcctttgata
     2161 atttttatat ttgcgtattc tttcgatttt tttgttaatt catctgatat tatctcagta
     2221 tttgaattta gatagttcat tgcatactca ccaaacattg gaaggatgtg caatcggaat
     2281 atatctttag tattactaat tgttacgtaa cttggagctc tttttgatct agtagtctga
     2341 cctagttcat atctaggaag ccattttgat tcatagaatt ccttaaattt tatttttcct
     2401 tttaattcta gtgaatttgc attctcctct cttaaaactg tttcaatttt ccttttcatt
     2461 gcattttcgg ctttaatagc caattgccta gttggaaata ctttttgata cttttctgat
     2521 gtaattccag tgatttctct gaattttttt ggatacctca gattagtttt atatttccca
     2581 tttgaaagtt ttgtaggcaa aattcctcac tcccttaaaa agtaaggcag tatatttcgc
     2641 tattcttagt atatgcggcg agaagaaatt ttcaaattgt tttaaatgta ttcataaaat
     2701 ctttgatatc ctctatgtca aagcgaacta taccgtcaat tctgattact ggaaaattat
     2761 atatacgaat ccacttatct aaagtattat tatcaattcc tagaaactca caggtttgtt
     2821 ttttattcaa gtagactctc aattttataa aatcatctcc taaaaaagat tagaattttt
     2881 gaattgcttt gggtaagcac ataggaattt cacctctccc cagtttacgg cgagccttac
     2941 ggaagtatca ttatttgctg ctattgtcat ggcattttgt tttcccagaa caaaaagaaa
     3001 tctctaagta tttgtcgcta atcaggtcat ggcgtacttg agtttctagc tcgaatagca
     3061 gggatcgtta ttcaaaaatt gtatttaatt gtcaatgatc aaaaataggt tttttcctat
     3121 tcactattaa taacgataaa catgtataaa aagacacact acgaaaaaag ttaaatggag
     3181 tttactgatt ttcatatttg tatatggaac agacatagtc ttgaataatt tttataaatg
     3241 ttttgatgct tatcaactta ttcaacccgc tatccacttt aagtataatt tttccatacg
     3301 ccaataaagt agttttagga gcatgttctc atttgagaac gctccttttt tatatatttt
     3361 gaaagctact tactatacgc gtagtttcaa atagttttgt cattctaatc ttcagcaaca
     3421 gaaaaaaatc taaattataa aatttagggt tgactaaaat ataatttttg tataccatat
     3481 gatcgtgata atcattatca tttagaagga gaaagttatg ttagaagtga aaaagcttaa
     3541 tgtttgttat aacgattttt tagcattgaa cgagatttct ttaaaaatac aagaaggtgc
     3601 tttaacaggt attattggtc caaatggtgc tggaaaatct acattgctta aagctatgtt
     3661 aaatattatc cctcatcagg gagaggtctc cattaataat gaagatatca ataaaaaact
     3721 ggtgaagatt gcatatgtag agcaaaaagc tgatattgat tttacttttc caataaaagt
     3781 aaaagaatgt gtatctatgg gaacatatgc cggaatgaaa gtttttcaaa gaattaaaaa
     3841 tgcagaatgg caaagagttt caaaggcttt ggaaaaggtc gatatgggaa aatattctaa
     3901 tcatcaaata ggagaattat ctggaggaca atttcaacgt gtacttttag caagatgtct
     3961 tgctcaaaat gcggatttca tatttttaga tgaaccattt gtagggatag acttagttag
     4021 tgaacgaata attatggata cgttaaaaga attgaaacat cagggaaaaa cagtgctaat
     4081 tgtgcatcat gatttaagca aggtaaagaa gtattttgat aatattattc ttttgaatcg
     4141 aaatctaatt gctcacggaa gtgtagaatc tgtatttaat gaagagaatt taaaaaaagc
     4201 ctatggagat actatattta ttggagagac taactatata gagtcaagta aaaattagcc
     4261 aaatcaaaaa atattttata acagataatt ccgaaaaaag ggtacaacaa ataagcttct
     4321 taccattagt ttttttgaat gttcaatagc taatcagaaa caattatata aggctcgttt
     4381 ttagtcaaaa tagcatgaat cacgtaacaa agcttacgag aaaccgcacc taaggcagtt
     4441 agatgatgtt ttccttcgga aacttttttc tggtaaaacg cactaaaaac aggatcacat
     4501 tgagaagcta ctaaagccgc cgaaaacaag gcttttcgca aatatggaga ccctcgttta
     4561 ctcataacat tatgcgttgc ctcatattgc cctgattgtg tcacggaagc gtctatccca
     4621 gcgtaagcaa cgagtttttt cgggttagaa aatttttgaa tatctccgat ctcaccaaga
     4681 atagtcgcac cgtttatagg accaattcca ggaattgtga gaataacaga atttaaagtg
     4741 tccattatgt gcttgatttc tctttccgtt tcctttattt ggctttcaat gaattttaat
     4801 tgttcaatca ttgaacgtag ttggaaggtg aacgcttctt gagcaaattt tactccaaag
     4861 gagttagaag ctgcttgctt caattggtcc gctttttctg ccttgattcg gttcctgctt
     4921 aactcagcta agtattgggc caaagtttct gaggaaatgt tttctaagtc gatcggggca
     4981 ctaaacgcca gcaataactc tttagaagct tttccgaaaa cgccctgctt ggtaaaaatc
     5041 gtcgcgtatt ctggaaagac ttgatccaat acggctataa tttttctttt gaagtcgctc
     5101 gctgtaccta ctaaataagt tcggtaacga gaaagttgtt tcaaagaaaa aacattttca
     5161 tcggataaga ccgtttcgac aaatgagcca tagcgcatta agtcagcaat caaaacagaa
     5221 tcaataatat cgttttttcg tttccgtatt tctgtacctt tacgccatcc gtctgtttga
     5281 aggggattca caacgtgaat caaataatct gattcgtgaa ggtatgaaaa gatggacagc
     5341 caatagtgcc ccgtggcttc catccctata acaaagtcac tgggtgctcc ggaatagtcg
     5401 accattcgct caattaatga ctctgctcct tcagtggtgt taggaaatga aaaacctttt
     5461 agtaatacct ttccttcctc gttcatgata gaagcaacgt gagtgcgttt accgatatca
     5521 atgcctaaaa agtacatgta tatctgacct ttcaaaaaaa gaattttgga ttgaaatcca
     5581 ctcaactgaa aagcgtcact accttgtacg agatacggag taaagtaagg actttcaaca
     5641 tctaactcat acgcaatcca cttatcagag agaggagcca gtctctcgag tgcgggttca
     5701 atggcccaag gggggaacgg cttagcctct caatccaata aatatattat ctcaaaagaa
     5761 atgagttaat accaaattaa aataacgatg gttcataggg ttatccaatc aaaaccggaa
     5821 ggttaaaacc ctaactctaa tatacaaggg ggaataatat gcttcaaaat tttattaatg
     5881 gtctgtatga ctttcacttt ttacagaatg ctctaattac atctgtagtt atagggattg
     5941 tttctggaac agtaggttgt tttattattt taagaggaat gtctcttatg ggtgatgcta
     6001 tttctcatgc tgttttgcca ggagtagcaa tttcttatat tttaggtatt aattttttta
     6061 ttggtgcgat tgtatttggt ctattagctt ctatcattat tacttttata aaaagcaata
     6121 gtgtaataaa aggagataca gcaattggta ttactttcag ttctttttta gcattaggag
     6181 ttatccttat aggagttgca aatagctcaa cggatttatt tcatatctta tttggaaata
     6241 ttttagctgt acaagatatt gataagtgga ttacaattgg cgtttctgtt ttagtgttgg
     6301 ttacagttgt tctatttttt aaagaattat taatcacttc ttttgatcca ttaatggcaa
     6361 aagcaatggg gatgaatgtc agtttttatc attatctatt aatgattcta ttaactttag
     6421 tagcagtaac agctatgcaa agtgtgggaa ctatattaat tgttgcaatg ttaattacac
     6481 cagctgctac agcatattta tatgcaaaca gcttaaaaac tatgatattt atttcggcga
     6541 cagtcggagc gtgctcatca ttgctaggtt tgttcattgg ttatagtttt aacatagcag
     6601 caggatcaag tattgtttta acagcggcat tcttatttgt aattagtttt ctaatctcac
     6661 caaaacaaaa atttttaaga aagaaggaga gaaagttatg atgagaaaat ggaaagcagt
     6721 actgggaagt ctggggattt tgattgctct ttttatattc ggggcatgtt caacaaatag
     6781 taaagacaaa gatacagtgg cttcgaacga aaaattaaag gtagtagtta ctaattcgat
     6841 tttagcagat attactgaaa atatagcaaa agataaaatt gatttacaca gtatcgtacc
     6901 tattgggaaa gatccccacg aatatgaacc tttgcctgaa gatgttcaaa aaacttcaaa
     6961 agcagatttg attttttata acggtgttaa cttggaaact ggaggaaatg cttggtttac
     7021 aaaattagta aaaaatgcga acaaagagga aaacaaagac tattttgcag caagtgatgg
     7081 catagatgtt atttacttag agggtcagag tgagaaaggg aaggaagatc cccatgcttg
     7141 gttaaattta gaaaacggta ttatttacgc taaaaatatt gaaaaacagt tagcggaaaa
     7201 agatcctgat aataaaaaat tctataaaga aaatctagat aagtatattg aaaaattgga
     7261 ttctctagac aaagaagcta aatctaaatt tgcttcaatt ccgaatgata aaaaaatgat
     7321 tgttacaagt gaaggatgct ttaaatattt ctcgaaagcg tataatgtgc cttctgctta
     7381 catttgggaa atcaacactg aagaagaagg aacaccagat caaataaaac acttagttga
     7441 aaaattacgc acaacaaaag ttccctcctt attcgtagaa agcagtgtgg acgatagacc
     7501 aatgaaaaca gtatcaaaag ataccaatat tcctatctat tcaacgattt ttactgattc
     7561 aattgcagaa aaaggacaag atggtgatag ttactatgcg atgatgaaat ggaacctgga
     7621 taaaattgct gaaggccttt cgaaataatt tggagggtag gtatggtaca aattaaaacg
     7681 actactatac taatttattt actagcaatt caggaattat caaaaagaaa aaagaaatta
     7741 aaaaataatg atttagcaaa agttctaagt gttagtccag catctgtaag tgagatgctg
     7801 gctaaactta gatatgagga ctatttagat ataggatttc aattaactga taaagggaaa
     7861 aaatttattg acgattacaa aaaaggtttt aagtaaaaga ctttattttt agggaggata
     7921 agaaaatggc tattggagtg attatgatag gacttaccct aatagctgga tataattttt
     7981 ttaaatcttt ctcggattat tttagaaaag gtgaatgatt tagatgagtc tattaattct
     8041 tgtaggattg ctttttgtat cagttatttt gctctttctc attagtttag ctctaaggaa
     8101 tgagttcagt aatagacgtt aacgattgaa acttcaagtc ctgtgtaaga taatatagtt
     8161 aattcatctt attaggagga agtacccata gaaaccagag attatattac aactcaccaa
     8221 aaattaatag aaagtggact aagccatttt gaaaaagaag gatttcagca aactaagata
     8281 aggcaactgt gcttgaccgc aggcgtaact acgggtgctt tttataaaca ttttacttct
     8341 aaggaagcgt tatttgaaga aattattcag ccgcatatta atgcgcttat taatatgtat
     8401 aatgaagagt ttaccaaata cctcttaaac ttagaaaata tagatataga gaaaagttgg
     8461 agagagaact tcaatgaatt agatccattt attaattata tttttgagaa taaacgtata
     8521 ttcgatttgg ttttattcca atcagctgga accaaatatc aagatattgt tgaaaaaatt
     8581 acatcatttg tgacaaaaaa gacagtctgt tgtctagata agttaaagac tatcggaatg
     8641 atcaatcaaa atttagtctt taatgaagaa gaaatccact tctacattta ctcattctac
     8701 gctacattct atgatatatt gaagcatagc tattcaaaag aaaagactat gctattaacc
     8761 agaagattgt atcaattttc tgttcctggg tggattgaat tactgagtta attaaaaaaa
     8821 agcttgcatt gagaattgtt ctcagttatg ctttttgata agatcgcact gttatctgaa
     8881 taacggtgca tttttgtcag cttaagataa cagtgcgatc ttattttttg gaggtgaaag
     8941 agtgtcaaaa atcaatccgt taaccttgat tactttaaat attttttttc cggtcattat
     9001 ttttttagga aagggaaata tatttcaagt ggggtgtttc attattgcta cagttttagt
     9061 attgatgttt cgtaaaataa agacatgtat ttatttcatc ttgatttatt cagttctgtt
     9121 tttagtaatg aaaattattg atgctatcaa gatagagttt tttactatat tttttgggac
     9181 atccttatat attgtatttc gaatgttgcc ggtcgtaatg atctcatggt tgttggttag
     9241 tgtctatact agcaatgaat tattatcctc tttagaaaaa atacatttac cgaaaaaaat
     9301 aatgttaagt attacagtag tgatcagatt ctttccgact tatcgttctg aaataagaat
     9361 gattaaagaa tctttaaaaa tgagaaacat tcgcttgtct ttttttcagc ctctgcaata
     9421 tttggaattt ctgttagttc ccgttcttat acgtgcaact atgattgctg aagaaatgac
     9481 cgctaatgca ataacaaagg ggatagaatc tcctgtgaaa agaagttctt actataaaac
     9541 aaacatgagc ttattagact atatcatcct gttttccgtt actatttttt tcttaagtat
     9601 tttgatttga ggtgaaagaa ttgtttgaat ttaacaatgt ttctttttca tataaggatg
     9661 ttaaggaaca agcgcttgtt gaccttaatt tttcaataaa aaaaggcagt cttacattga
     9721 ttactggttc ttctgggagt gggaaatcca cactattaaa gttaatgaat ggacttattc
     9781 caagacttta tgacggaaaa ttggttgggg aaattttatt tgaaaataac tcgctacaat
     9841 ccttaacaca agaaagtata tcaaaaaata ttggctacgt aagtcaaaat cctagaggac
     9901 aattctttac aacaaataca acgagtgagt tagtattttc gatggaaaac tttggattta
     9961 caaaagagca tatggataaa agaatcaatg aattagctga attattagat ctttcaaata
    10021 tattagataa aaacattttt tcgctatcaa gtggtgaacg gcagaaaatt tctattggat
    10081 gtagtttatc gctaaactct aaattaatta tattggatga accgtcttca aatttggact
    10141 tcaaagcgac taagaaactt gctgacttac tttgtgattt aaaaaataaa ggatttacta
    10201 ttgttatagc tgaacatcgt ttttactatt tgaaagaatt aattgatcaa gttctatttg
    10261 ttaaaaatag aactgtaaaa actacaacta ttcagttgtt aaaaactgaa aacaataacg
    10321 cattaaggaa atttgacatt tttaatagtt cgattgaagt tccctatatc gaaaaggtaa
    10381 gtgcagatgt attaaaagtt gaagaaatta cttatgaaaa tattctaaaa gacataaatt
    10441 ttgatgtcaa agttggagat gttattggtt tggtagggaa aaacggtgtg gggaaaacaa
    10501 ccttactaag aatactcatg aagattattc gccctaccaa aggaaagata atcgaaaata
    10561 aaaaaaattt gtcatcgccg tttttagtaa tgcaagaaat ggattaccaa tttttcacag
    10621 aatcagtgag gagcgagtta gccctgggga atgaaatagt tagtaaagat cagcaagaaa
    10681 aaattttaaa aaaaatggaa ctttataaaa tgaaagatca attaccgttt gatttatccg
    10741 gtggagaaaa acaaagatta ttggtttcta tagcttcatt gtcaaaaact aatttatttc
    10801 tatttgatga acctacaagt gggttggatt atatcaatat ggaaaggatt gctgacctta
    10861 ttaaagactt aaaagaaaag ggtgcagtag ttatagccac tcatgatccg gaatttcttt
    10921 ataaaacatg caatagaatt atttatttaa aaaatggtgg aataaaaaaa gatataaaac
    10981 ttagagtaga agattcaact ttgatagaac agatttttaa tgacataacg aaatagaatt
    11041 gaggagaata tatatgaaaa caaaagatta tatttttatt ggtttagcta cagttatttc
    11101 cctagtaatt tacttttttt caataatggt tagtagtatt ggtggggcgt ttggtcacag
    11161 catatcccca gggatttttg gattattatc tggaatcata tttgtttata ttagctataa
    11221 ctaccctaga aaaggaattt ttactattta cacaattgtt ttattgttat tttttacgtt
    11281 aatgggcggg gcgtacttac cttggtttat cagttcgatt agtatggcga tcttagctga
    11341 tattattcta tctgtatttg gatatgatcg agcaattcct caagttgtgt cttgggttct
    11401 aatgcaacta ggcagtgctg ccggacagtg gattcccata tggttcttta cagatcgttt
    11461 tcgtcaagac tggatagata gaggacaatc tgctgcaaca atggatgcaa tgatccacta
    11521 cgcggtgggt atatggggaa ttatttcagt gttagttgta gcaagtttat ctatgattgg
    11581 tgtattgatt ggaagaaaag tcttaaaaaa atacaaaaaa tagaacgtag tttaccttct
    11641 atctgtttgt acatctctaa tttacgctat tacggaaagc attaacagtt gtaaacgaat
    11701 aagagaagaa attgaatgcg ggggaggaaa aaatgaaagt atacaaaaaa ttatttagtt
    11761 atgtccctaa aatgaaatac tacggatggt tagccacttt attttctgat ttttctgtcg
    11821 tattaacagt ctatggatat tacagtattt acaaatttct tcagtctcta attattgtag
    11881 gagatgaata tttagctaag agttatgcgg ttcaaactgt tgcttggtta actttaggag
    11941 ctttatttta tattttttct ggcttatttt ctcatatcct tgggtttcgc ttagagacta
    12001 atttgagaaa aaagggaatt agcggtttaa cagaagcaag ttttcgtttt tttgatttaa
    12061 attcttcggg atatattcga aagacaattg atgacaacgc ggctaaaaca catatggcaa
    12121 ttgctcactt gattccagat agtgctcaag ccatagtaac acctgtttta tcagttgcct
    12181 taggcttttt cattaacttt aaaattggat tggttctaat cgccatgtta gtaattggtg
    12241 tattcttgtt aaagaaaatg acagagaata caaattttat tcaaaaatat caagaatcgt
    12301 tagatgtgtt aagctctgaa acggtagaat atatccgcgg aattcaagtt ataaagatat
    12361 ttggtgctaa agtaacatca tttaaagcct tgaataaagc aattaacgat tatgctaagt
    12421 ttgcatataa gtattctttg agtgggaaaa caccttttgt tctttttcaa tggctatttt
    12481 ttggcatagt ttgcattttg atagttccta taaccttttt tataacttct cttaccactc
    12541 ccaagatctt agctgttgac ttgttgatgt tgtttttttt aagtggaatt atttttgttt
    12601 ctttcatgag aataatgtat gtaagtatgc acttgtttga agcaagatat gcagtcgaaa
    12661 cactagactc cctttattta gaaatgaagc aagaccaact aaaatatggg aacgagacac
    12721 agattaaaaa ttgtgacatt gcatttgaaa atgtcagctt ttcatacaaa gaagagtgtg
    12781 ttttagaaaa tttcaactta gttttagaac aaaaaagaat ttatgcatta gtaggaaaat
    12841 caggaagcgg aaagagtacg atagctaaac taatttcagg tttctacaat gtggataagg
    12901 gacagattaa aattggtggt aagcctttaa atcactattc aaaagacaca attattaaca
    12961 caattgcttt tgtctttcaa gatacgaaac tattcaatca gagtatttac gataacgttt
    13021 ctatagcaaa tcctcaagcg ccagcaagta aagtatttga cgcgctaaga ttagccggtt
    13081 gcgaatcgat tttagaaaaa tttcccgaaa gagagcatac cttaattggt agtaaaggag
    13141 tttatctgtc aggaggagaa aaacaacgta tagctattgc cagagcaatt ttaaaagatg
    13201 caaagattgt tattatggat gaagccagtg catctattga tccagataat gagtatgaac
    13261 tacaaaaggc ttttcaatat ttgatgaaag aaaaaacagt tattatgata gctcatcgat
    13321 tatccagcat taaatctgta gatgaaattc ttgtcttgtc agaaggaaaa atagtagaac
    13381 gtggaaatca taatgaatta atggctaaac atcgagtgta taaacaattg gtcactaatt
    13441 atgagaatgc aaatgattgg aggcttgaca atgaaaaatt tttataaaaa aaaatttgct
    13501 ttaactgatc agggcgcaga agctttaact aaagcttcta tttctagttt ttttgtatac
    13561 tgcattaata tggtaccagc attcattata atgatgctga tagacgaatt agttttagaa
    13621 aatgcaaagc cccgttggct ttattttgca gtatcgtttg ttactttgct tttcatgtat
    13681 tggttgttag atagggagta tgaaaactta tataatagta cttataaaga aagtgcgcat
    13741 ttaagagtgc aaattgcaga cgatttgtca aatttaccat tatcctattt ttcaaaacat
    13801 aatttatcag atttatctca aactatcatg tctgacgttg aaggtattga gcatgcgatg
    13861 agtcatgcaa tacctaaagc cggcggtatg gctctgtttt tcccttttat ttcagtgatg
    13921 cttttggttg gtaatgtcaa aatgggatta gctgttattt tgccaacgtt atttagtttt
    13981 gtcttaatct tgttatcaaa gaaatcccaa acgaaagcca atactaaata ttacgatact
    14041 ttgagagaaa actcggaaga atttcaagaa actattgaat tgcagcaaga gattaatagc
    14101 tttaatctat ctaaaaaagt tcaagacaga cttttcaaaa aaatggaaga gagtgaaagg
    14161 attcatttaa aggtagaatt aagtactttt tcagtcatgg ccttatcctc tattttctca
    14221 tatgttagtt tagcagtagt cattctagta ggtgttcact tactgttaac gggtgaagtg
    14281 actatactct acgtcgttgg ttacttacta gccgcaataa aaataaagga ttccttcgat
    14341 tctatgaaag aagcagtgct tgaaatattt tatttagctc ctaaaataca gcgaattaga
    14401 gctatgaagg aaaccagtat ccaagaggga tcagatagtc ctctgaaatc ttttgacatt
    14461 gagttgcgag atgtctcttt ctcatatgat aacaataccc caattttaga tcatatctct
    14521 tttacggcta aacaaggaga agtgactgcg ttagtaggtg ctagtggttc cgggaaaact
    14581 agtatcttga aattagtgtc tagactgtat gattatgatg aaggctgtat tttaattgat
    14641 gggtatgata ttaaacgtgt atctccagct tctttgttta gcaaaatcgc tattgttttt
    14701 caagaggtaa ctttgtttaa tacaagtatt ttggaaaata tacgtattgg aaatagtcaa
    14761 gcgtcagatg aggaagtgaa aaaagcagct cgtttagcta actgcgaaga ctttattgaa
    14821 aaactacctg acggttatca tacattggtc ggagaaaacg gaagttcact ttctggagga
    14881 gagcgacaac gcctatccat agcacgggct tttttgaaaa atgcgccaat tttaatttta
    14941 gatgaaatta ctgccagttt agatgcggaa aatgaaaaac gtattcagga aagtttaaat
    15001 cgtttaatac aagataagac agtactaatt atatcgcatc gcttgaagtc tattgaaaag
    15061 gtaaataaaa ttgtagtgat ggatcaagga aaactagttg atcaaggtac tcataatgag
    15121 ttgtatcgaa ggtcagaaat atacaaaaat ctcataaaga aaactaaact atctgagaaa
    15181 ttcgtctatg aaaaagaagc tcaatcacga tgatgaaata ggctttaaat taacctacaa
    15241 tttttgggat atagttataa ttctttttca aaaagaataa ttttattaaa cagcatctaa
    15301 ttttgaatac acgaaa
//
LOCUS       DAFBQH010000070        14562 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.072, whole genome shotgun sequence.
ACCESSION   DAFBQH010000070 DAFBQH010000000
VERSION     DAFBQH010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.072"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..129)
                     /locus_tag="LK694_002185"
     CDS             complement(<1..129)
                     /locus_tag="LK694_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBK5053027.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDS"
     gene            402..2873
                     /locus_tag="LK694_002186"
     CDS             402..2873
                     /locus_tag="LK694_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK5053028.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2976..4712)
                     /locus_tag="LK694_002187"
     CDS             complement(2976..4712)
                     /locus_tag="LK694_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBK5053029.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4709..5413)
                     /locus_tag="LK694_002188"
     CDS             complement(4709..5413)
                     /locus_tag="LK694_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5053030.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5544..6047
                     /locus_tag="LK694_002189"
     CDS             5544..6047
                     /locus_tag="LK694_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBK5053031.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6007..6345
                     /locus_tag="LK694_002190"
     CDS             6007..6345
                     /locus_tag="LK694_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBK5053032.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6366..7784
                     /gene="ffh"
                     /locus_tag="LK694_002191"
     CDS             6366..7784
                     /gene="ffh"
                     /locus_tag="LK694_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBK5053033.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7896..8474
                     /locus_tag="LK694_002192"
     CDS             7896..8474
                     /locus_tag="LK694_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBK5053034.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8478..8999
                     /locus_tag="LK694_002193"
     CDS             8478..8999
                     /locus_tag="LK694_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBK5053035.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9078..9632)
                     /locus_tag="LK694_002194"
     CDS             complement(9078..9632)
                     /locus_tag="LK694_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBK5053036.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9885..10160
                     /gene="rpsP"
                     /locus_tag="LK694_002195"
     CDS             9885..10160
                     /gene="rpsP"
                     /locus_tag="LK694_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBK5053037.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10172..10423
                     /locus_tag="LK694_002196"
     CDS             10172..10423
                     /locus_tag="LK694_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBK5053038.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10548..11339
                     /locus_tag="LK694_002197"
     CDS             10548..11339
                     /locus_tag="LK694_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053039.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11509..12030
                     /gene="rimM"
                     /locus_tag="LK694_002198"
     CDS             11509..12030
                     /gene="rimM"
                     /locus_tag="LK694_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBK5053040.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12020..12781
                     /gene="trmD"
                     /locus_tag="LK694_002199"
     CDS             12020..12781
                     /gene="trmD"
                     /locus_tag="LK694_002199"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBK5053041.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12917..13264
                     /gene="rplS"
                     /locus_tag="LK694_002200"
     CDS             12917..13264
                     /gene="rplS"
                     /locus_tag="LK694_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBK5053042.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13522..13815
                     /locus_tag="LK694_002201"
     CDS             13522..13815
                     /locus_tag="LK694_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBK5053043.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         4880 a         2616 c         2945 g         4121 t
ORIGIN      
        1 agagtcatcc aattttcctt taataaactg cgcttgaacg cctttatatt ctttttcaat
       61 cagccattct tcatcaaaaa ataaatgttt atctgttaat ccgagtaatt ttctagtatg
      121 attatccatt gagagactcc tttggtagga tgacgttttg gtcgactaaa ttttaccaaa
      181 agtgagtctc tttttgagtt gaaacgaaga aaaagtgtta gtagaaaatc gccttcgatt
      241 ttccaccaac acaaaatatt atagaaccct ttttttttgc tatatgacag gaatgaaagg
      301 tgtttgcaag cttaatgtga ggaacacttt ccatatctgt tctatactgg ataggagaag
      361 tttggaaata tccagaaaac gaaatcaatc gaggtagcca catgaagaat aaatggataa
      421 atcgaagtaa aaaaaagaaa gtaacacaaa gatcatcaga taaagtcatc cgtctttttc
      481 catcgtataa agaaggatta acggcagatc aagtcaagga acgaatagaa aaaggtgcag
      541 caaataattc agtcgatccc acattcaaaa caaatcagca aatcgtattg gaaaacatat
      601 ttacgtattt caatttgatc tttcttatct tagccatttt gctttgttta gtagaatcct
      661 ataagaactt gactttttta ccagttatca tagccaatac aggaatcgcc atttttcagg
      721 aaattcgatc caaaaagatc ttggacgaac taaatgtttt aaatacagca aaagtcaatg
      781 tcatacgaga cgggatagaa caaacaatag atatagaaga ccttgtaata gatgacattg
      841 ttattttaga gactggatat caaattccag cagatgcaga ggtcgtggaa gggaaactgc
      901 aagttaatga ggctttattg acaggcgaag cagatgaaat aaccaaagaa ataggtgatc
      961 cattaatgtc cggcagtttc attgtttccg gaaaagctta cgcccgatta gacaaagttg
     1021 gagcagattc gtatatttcc cagctgacag tcaaagccaa gacaatggga gaaggagaac
     1081 agtcggagat gattgcttct cttaatcaat tgatcaaatg gattgggatc atcattattc
     1141 ctatcggtat cctgcttttt tctcaaagtt acttttttaa tggaaacacg atcaaagaaa
     1201 gtgtcgttgc aatggaagca gcattgatcg gtatgatacc tgaaggactg tacttgctga
     1261 cgacgattgc attagcatta agtgcgacta aactagcaaa gcaaagagtt cttttacaca
     1321 atatgaaaag cattgagaca ttggcaaggg taaatgtcct ttgtgtagat aaaacaggaa
     1381 cgatcacaga aaataaaatg tctgttcaaa aaatgatcgt ctctaaaaaa caagaaataa
     1441 tcagtcagtc agatcaattg gaagcatgca ttgctgacta tgcaaaagcg atggcaggag
     1501 acaatgcaac aatggaagct gttaaagcat attttacaaa aaccactgat caatcctatc
     1561 accatgttat tcctttttct tctgtccaaa aattcagcag tgtagcgttg gcagacaagg
     1621 tgtatgtttt aggagcgccc gaaatggttt tgcgcgatca attgccggaa tatgcggaag
     1681 agtttgtttc tttcgctgaa aaaggctatc gcgtgttggt ctttggtgtg tacgatggga
     1741 ttcttgaaga accagtattg acagaagcag tcacaccatt aggctatatt ctgattgcta
     1801 atcctatcag aaaagaagca agagccacat ttgattactt caaaaaacag caagtagcca
     1861 tcaaagtgat ttctggagat aatccgctca ccgtatccaa tgtagctgtc caagcaggga
     1921 tttcacatgc tgatcagtac gttgatgcat ctaaattatc agaagaagaa tatgaagcag
     1981 ctgtcgaaaa atatacagtt tttggacgcg tcaagccaga acaaaagaaa atcttcgttc
     2041 aattgataaa gcgtaacaat acagtagcta tgacaggtga tggcgtgaat gatattctag
     2101 ccatgaaaga agcagactgc agtatagcga tggcttccgg aaatgaagcc gccatgcagg
     2161 cctcacaagt ggtcttactt gattctgact tttcacgcat gccagaagtt gttgcagaag
     2221 gacggcgcgt tgtgaacaat attgagcgat cagccagctt gtttttagtg aagaatattt
     2281 tctccttttt gctctcactg ttttcagtga tctttgctct gacctatcct ttagaaccat
     2341 cacaaatcac attgattagt ttgtttacta tcggacttcc atctttcttg cttgctctag
     2401 aagaaaacaa aaagcgaatc agagggaaat tcatcatgaa tgtgatggaa aaagctgtgc
     2461 caggtggact aacagatatg atcgtagtcg gtgcgttggt gatctgcggg gtgactttga
     2521 atctaaataa aacagatgtt tctacagcat ctaccatgct tttgattgca gtagggttcc
     2581 tcgttctcta taaaatctgt tcaccgctga ataaattcag aagtcagatc attcttttct
     2641 gtgctagcgg taactttttt tcggtcatct ttttgcataa gctgttttca atcaccggta
     2701 tctcagctgt atcacttttg ctgttgagca ttctattttt ctcggcagat tcgattttta
     2761 ggcatttgac cacattggta gaatatttgt ttgaacgtaa aaatgaaaaa gagcaaaagc
     2821 cgtttagtcg cgtagctaaa aaatttctat cgatatttaa acgagaaaat tgacgaaaag
     2881 tatctcaaaa aagagtaaaa aagaacaacc actcgtgtat ctttcgaagc aggatgagtc
     2941 atccaacttt aaaacgcacg agcagttgct cttttttaca ttttcggaat ggtaacggtg
     3001 aaagtcgaac ctgttccaag atgactgtct acagtaatcg taccattaag gagttgcacg
     3061 tagtctttta caatcgctaa ccctaatccg gtcccgcctg aatgtcggct tcgcgccttg
     3121 tctacgcggt aaaaacgctc aaagatccgt tcctgatcct caatgtcgat acctatacca
     3181 aagtcttgga cagaaaacga tagatcatct gttgcctgat agcggataat aatctcgctg
     3241 tcgctttttg aatattggac agcattttca atcagatttt tgataattgg ataaaggata
     3301 tctgttttag tagtaaatgg ttcgttcttt ccaatcaatc gaatcgtcaa ccgtttcttt
     3361 tcgatgattg gctgataatt ttgaacgatt tgctggaaat aaggttctgt ataaattgtc
     3421 tggatctcgt aagaattctc gccatctttt gacagctgga tgatttcccg aatcagttta
     3481 tctaaccgga tcgcatcttt ttgcatgatc tcaagaaaag aagtcagagt ttgcgggtca
     3541 tcttttgcac catccagtaa agtctcggta aaaccaatca gtgaagtcac aggagttttt
     3601 aattcgtgag agacgttgcc aacgaaatcc ttttgtagct tttctaaacg tcgcactttc
     3661 gttaagtcat acgccacccc tagaatctgt ccggtacctt ctgaattagt aaagtagcgc
     3721 aaactcaagt ctaatgtctg ccgaccttct gtaagggtga tctcctgatg gatcagcgga
     3781 tgctcaggag tcacttgatg aatcagctga atgagttttg gctcttgaat gacttctgtg
     3841 taccgctgtt caggttgata gcttaccacg tttaaatgta gctgcatctt tggattaagc
     3901 agtagtaaac gagaatcagc atcatcaatc aggaatacgc caatcatcag ttcgttcagt
     3961 aacgtatata attgctcttc tgtggtagta cacgcccgat acatcttact catttgttcg
     4021 ctcaacgcat taatcgtctg atacaattct tcccattgat cgggtgtttg catgattact
     4081 tccgcctgat taggattttg caccattttt tttagtacgg gcagcacggt tttcaatggc
     4141 tggttttttt gatagatcag ataatagatc attcctgcaa gaacaaggaa gaaagacaga
     4201 aagaagaaaa agacccaacg gcgaaaactc tcagttctcg gtaaaaaaac gcttgtcggt
     4261 tctgcgatac gtaaaatggc ttctaactgg ccactttttt tgactggtaa tgcgacatat
     4321 aaaagatcct cattcaaagt agtacttttg cgcaaagcgc tacctaggtt cccaccattc
     4381 aatacggctt tgatttcggg acgggagttt cgctgttcat tcaatgtttc attgtttgtg
     4441 tcaaataaga tctttccagt agcatccatt aaagtaatcc gttcgttcga ctgatggacg
     4501 aattcttcca aagcagtttt gttttgcgct gcttggaggt tttcgacatc taattgccgg
     4561 atcagcagtt gtgctttttt tgtcaggtag ctttcttgct gttccacgat ctgctgttga
     4621 aaataatggg aaatcaattg ccagccacca aaaaacaaac taactaaaag aataagaaag
     4681 aagagacgtt cataaatttt tttccatttc atcgtttggg ttcctgaaac ttatagccaa
     4741 atcctctaat agtcaccaag tactttgggt gttttggatc tcgctcgatt ttttctcgca
     4801 aatgactaac gtgtacatct acgattcggc tttgtcctgc gaagtcaaaa ttccaaatgc
     4861 ggtctagcaa tgtgtctcgg tcgatcacac ggtcttttcg tttcatgaaa taaacaagta
     4921 attcaaattc tttcggtgtt agttcaatca gatggtcgtc tacggtcact tgataattgg
     4981 tcaaatcagc agtgatttgt ccaatcgata gatattcagg ctcagcttca tcttttgatt
     5041 gcttacgagg ttcaattcgt ctgaatattg ctttcatacg ggctaatact tcgcgaggac
     5101 taaatggttt ggtcaaatag tcgtcagcac ctatttctag gccaatgatc cgatccactt
     5161 ggtcatcttt agccgtcagc atcaaaatcg gtgtttcatt tttttcttga cgcagccgct
     5221 gagtgatctc catgccatcc atcgttggaa gcatcacatc taaaatgatg aaatcaaatg
     5281 attgttctaa tgctaattct aatccttcgc ctccatctgt ggcactggtt accttgtagc
     5341 cttctttttc caaattgaac gtcaagagcg ttacaataga aggttcatca tcaacaacta
     5401 gtattttttt cattcaaatc tcctttaaat aggtttcatt ctgtttatat tttagcatag
     5461 aatcagtaca aagggctatt ttacaaaaga aataaatggt ataataggaa ggcactttgc
     5521 tgaatagaaa ggaagagcag tcaatgattg gaatcagtgc atgtttaggc ggtctggcct
     5581 gtcgctatga tggacagaaa aaagaagtac cagaattgat ggaactagtt gaaactggag
     5641 aagctgtcat ggtctgtcca gaagtgatcg gcggattacc aattcctaga gatcccgctg
     5701 aaatcattgg tggaaatgga ttcgatgtgt ggaaagatca agcaaaagta tggacgacaa
     5761 gcggcgaaga tgtaacggaa gcgtacaaaa atggcgcgat caaagcctat caaaaattgc
     5821 aggaaaaaca cattgatcta gtcatcatga aggaaaaaag cccttcatgc ggaacccatt
     5881 cgatttatga cggaagtttt tccggaataa aaaaaaatgg tgccggagtg gctactgctt
     5941 attttattca gcaaaaaatg actgtgctct ctgatgaaga atggctgaaa actagaggtg
     6001 agcagaatgg aaatcgaaaa gaccaatcga atgaacgcgc tctttgaatt ttattcgacc
     6061 ttattgacag aaaaacaaat gaactacatg gaattatatt atgcagatga tttttcatta
     6121 ggtgagatcg cggaagaata cgaagtaagc cgacaagcag tatatgacaa tatcaagcgg
     6181 acaagcaaga tcttggagga ttatgaaaaa aaactccatc ttttttctaa ttatattgtt
     6241 cgagaacagg tattggaaaa tatgactgca tatattacag ataagtaccc ggaagataaa
     6301 aaactacttg aatatgtaca gcaaattcaa gcaatcgaag aataaataaa aggaatgaag
     6361 aaaatatggc atttgaaagc ttaactgaac gtctccagca ggcgatggga aaaataagaa
     6421 aaaaaggaaa agtatcagaa gctgacgtaa aagaaatgat gcgggaaatc cgccttgcct
     6481 tactagaagc cgatgttaac ttgcaagtag tcaaagattt tactaagcgc gtacgcgaac
     6541 gtgcggtagg ggcagaagtg ctggaaagtt tatcacctgc acaacaaatc gtaaaaatcg
     6601 ttgatgaaga attgacgatc acacttggtt ctgaaacagc agagttaaat aaatcaccaa
     6661 aaataccaac tgttatcatg atggctggtc ttcaaggggc aggtaaaacg acctttactg
     6721 gtaaattagc caattattta aagaaaaatg aaaatgctcg tccgctccta atcgcagggg
     6781 acgtatatcg accagctgcg atcgaccaat tgaaagtatt aggtcaacag ctagacgttc
     6841 ctgtatttga tatgggaact gaagtcagcc cggtagagat cgtccgtcaa gggatggagt
     6901 tagcaaaaga aaagaaaaac gactatgtat tgatcgatac ggcaggacgt ctgcatatcg
     6961 atgaaacatt gatggatgag ttgaaacaaa tcaaagaatt gacgcaacca aatgagattt
     7021 tgcttgttgt cgatgccatg actggtcaag atgccgtcaa cgtagctgat agcttcaacc
     7081 agcaattagg tattacaggg gtcgtcatca ctaaacttga tggggatact cgaggcgggg
     7141 cagcactatc gatccgctca gtgacaggcg caccaatcaa attcatcggt tctggtgaaa
     7201 aattgactga cttagaagta ttccacccag atcgtatggc tagccgtatc cttggtatgg
     7261 gggatatgct gacattgatt gaaaaagcgc agcaagacta cgacgagaaa aaagcggaag
     7321 aacttgcaca gaaaatgcgg gaaaattcat tcgatttcaa tgactttatt gaacagcttg
     7381 atcaagtgat gggtatgggc ccactggaag atctaatcaa aatgatccca ggaatgaatc
     7441 aagtccctgg aatcgaaaac gtcaaggtag atccaaaaga tgttgaacgc aaaaaagcga
     7501 tggtttattc tatgacgcca gcagaaagag caaatcctga tctgttgaac ccaagccgtc
     7561 gtcgccgaat tgctgccggt tctggaaaca gtgttgttga agtgaatcga atgatcaaac
     7621 aattcaagga atcaagaaaa atgatgcagc aaatgtccaa aggggatatg aacatcccag
     7681 gtatggacca aatgtttggt tcaggcgtca aaggaaaact aggcaaaatg gcgatgaatc
     7741 gaatgatcaa gaaaaataag aagaaaaaga aaaaacggaa ataagatgaa aaaaacagct
     7801 tcggtacaga ctgaaaatgt ctactgccgg agcttttcta tcagcaattt ttattcttta
     7861 ctaatacaat tactctaaac gaagaggtgg cacagatgag atgtccgtgg agtgaacaaa
     7921 cagaaagtat gcggcaatat catgatactg tctggggtgt tcctgaatat gatgaccaaa
     7981 agctgttccg aaaattgatg cttgatatta atcaagccgg attgagctgg cagacgattt
     8041 taaataaaag tgaagcattc gatgaagcat atgatcgatt cgatatcaaa aaagtcgcaa
     8101 attatggaga aacaaaaatc agtgaactaa tggaaaacaa aggaattatt cgtaaccgaa
     8161 gaaaaatcga agcagccgtc caaaacgcca aagcagtctt gaaaattcaa gaagaaatcg
     8221 gcagtttttc agagtatctt tggtcgtttt ctaatggaaa aatcgttgat tcttcttatc
     8281 attcacaaga agaagtccca acaacaagtc ctctttcaga tgaattagca aaagatctaa
     8341 agaaaagagg atttaaattt atcggaagca caacaatcta tgctttttta gaagctgtcg
     8401 ggatcatcaa tgatcacctt gacagttgtt tccgcaaaga agaggtcgta cgtatcggca
     8461 gaaagggcga ataaagcatg cgaaaaatca tattttatgg tgctataagt ttagatggct
     8521 atttggcaac gaaaacggat gatcttcaat ggctttttga cactccaaca ggtaagcaaa
     8581 ctacttatga agctttttat gaaacaatcg atacgaccat catgggcaga aaaacttatc
     8641 aggaagcaaa aaaatatttg gagaccgaag caatttatcc agagaaaaag aatttcgttt
     8701 tttccaccca cgcacaatta caattgccag atgctgaggt cgtaaacgaa gatcctgttg
     8761 cttttctaaa aaatctacaa atgacagaag gaaaagatat ctgggttgtg ggtggcggtc
     8821 gattgatcaa accgctattg gaacatcaaa tgattgatga atggtatatc cagattaccc
     8881 ctgtattatt aggggacggg attcctttat tccaaaaagg aacatatgaa gcgcgttttg
     8941 aaatgctaga cacgacacga ttcggtgaat ttatcgaatt gcattatgta cgaaagtaga
     9001 agtaaataaa ataaaaaagg caactatatg cagaaatgtc ctgtacatag ttgccttttt
     9061 tattaccgtt atgcttttta ctctgttaat ttgttagaaa aatctagata cgcatctaaa
     9121 atcgtttgga agaaacttaa tgttccttca acgatattac cattttcatc aattagattc
     9181 gcaacgtttc cgatatatgc ttccggttgc tgcaaggttg gcacattcaa aaagacaagt
     9241 gattggcgta gatggtggtt tgcgccaaag ccgccgatcc cacctggtga tgcagtaacg
     9301 actaaacctg gtttatgatc ccaaacactg tgtccataag gtcttgagcc tacatctaaa
     9361 gcatttttca atactgctgg cacagaacgg ttgtattcag gagtaacgaa aacgacaccg
     9421 tctagtcccc tcacttcctc acgaaaacga gtccattcag caggagcctg ttctggagtt
     9481 tcgagatcct cattgtaaaa tggcagatca tcgattttta caaatacact ttcgtaccct
     9541 tcaggaagca atttcgcaaa agcttctgct acttttttgt tgtaagaatc ttttcttaaa
     9601 ctaccaataa aaaatccaat tttttttgtc atacgatttt tcctccttca aactatctat
     9661 aataatctta caaaagataa gtaactggag caaataaaaa cacttactga caaaagctac
     9721 atagaagata gattacttat ttttaacaaa aaagaagtct aaaatcttct attttctaaa
     9781 aaaaatcgaa tttaatcaag ctgtaaagaa aaaaagcttt acagggtaaa aaaaaactgt
     9841 tacactacat cttgtgaata aaacaatgga ggtggaaata aataatggca gttaaaatcc
     9901 gtttaaaacg tatgggttct aaaaagagtc cgttttaccg tatcgtcgta gctgattcac
     9961 gttctcctcg tgatggacgt ttcatcgaaa ctgtaggtac ttacaatcct ttgaaagacc
    10021 ctgcagaagt agttttaaaa gaagacttag ttcttgactg gttatcaaaa ggtgcacaac
    10081 cttctgacac tgtacgtaac atcctttcaa aagaaggcgt tatgcaaaaa caccacgaag
    10141 ctaaattctc aaagaaataa ggtgaccgga tatgaaagat ttaagcgact tagtcttaac
    10201 aatcgttcgt ccgctagtca cttatcctga tcaagtcaag ttagacgtcg tagagtcgaa
    10261 agacttttac gagtacaatc taacagtggc tcctgaagat attggccgta tcattggaaa
    10321 acaagggcgt gttgctaaag caatccgcac aatcgtttac ggtgttcgga tcaatgcacc
    10381 gaaaaaagtg cgtttgaata tcatcgatga taaggaaaag taaataaacg tgctgtactc
    10441 gaattttcga gtgcagcttt tttgtttttt ttattaattg aagaaatgat tatttggcaa
    10501 aacaagcaag aaagcgtatc cttataggag gagggagtga acagccaatg agaaagaaaa
    10561 tgttatttat tccatttgct ctattgttaa gcgcttgtag ttcggttact caagaatcag
    10621 acaatacaca tctatcaagc agtatatctg aaactgttac cagtccagaa aaaagtagta
    10681 ccaaaacggc ttccaccact gaacaaacaa tggctacctc aaataatgag aaaaagactg
    10741 ctttagacca gttgaaagaa caacagccaa atgttcccat gcctctagat gtaccagtct
    10801 cttcaggcta tctcaatatc gcagcgacac atacaaagca aggttattct attttatatt
    10861 acagaacaga tcgtcccctc gggttgaatg ctgacgaatt gaatcaagaa acgcctattg
    10921 caacctatct atatcaatat gggtttgctt ctagtcaaga aacaatccaa gttttgcagc
    10981 catttgagat cgacacaaat ggtcagcaag tggacttagg ttctcgaatc acagggtatc
    11041 agcaaggtgc agcaggttca agttttcttg aatggcaaga aggaaactgg cgtattcgaa
    11101 tccgtgggaa taatatagaa ggccaagatc ccttactatt agctaaagaa atcgtagctt
    11161 atttggaaga aaacagtcta ccagcacctg aacaatttgg taaaattact gttgatatgg
    11221 gtgatacgac gaatcgagcc gtagaagtaa gctggcaaga accgaaaaat gcctatacga
    11281 tcactcatca agatcctatg tctgcgttga agatggcagt ttctatgaag aggttgtgaa
    11341 atcagcgcgc tgatctgagc ccatcattta actccaagtc acaggaagta atcaaaaata
    11401 gcacgccgca tttttgcata attttggctt aataccttaa aagaaaacta gaattgcctc
    11461 ttcacacatg ctaaaataaa acagacaaaa aagaaatggg gatataagtt gactgaatac
    11521 ttgaatgtag gaaaaatcgt aaacacacaa ggtatcaaag gcgaagtccg tgttatctca
    11581 acaacggatt tcccagaaga acgttacaaa aaaggaacgg tgctaacact attccaagac
    11641 ggaaaagcac ctgtggaatt aacagtcaaa agtcatcgca aacataaaaa ttttgactta
    11701 ttgagttttg aaggacatcc ttctatcaat gatgtggaaa aatatcggga tggcatatta
    11761 aaagtgtcaa aagacaactc tgtagatcta gctgaaaatg aattctatta ccatgagatc
    11821 atcggtctgc atgtattaga agatggaact gagttaggta aagtaaaaga aatcttgtca
    11881 ccaggtgcca atgatgtttg ggtcgttcaa cgtccgaaaa aagctgatat attgcttcca
    11941 tatatcgctt ctgtcgtcaa agaagtcgat ttagaagctg gagtggtccg tgtagaaatt
    12001 ccagaaggac tgattgacga tgaagattga tgtattgact ttatttccaa gaatgtttga
    12061 aggtcctatg ggggaatcca tcatcggtaa ggctgtagat aaaggattat tagatatcaa
    12121 tatctctaac ttccgcgatt attcagataa caaacatcag acagtagatg attatccata
    12181 cggcggtgga gcaggtatgc tgctcaaagt ccagcccatc tatgataaca tccaagccat
    12241 tgaaaaagct gcaccggatg tcaaaaaaag agtaattctt cttgacccag ctggtaaacg
    12301 attcgatcag aaaatggcag aagaattttc acaagaagaa cacttagtat tcatttgtgg
    12361 tcattatgag ggatatgatg aacgtatccg cacattagtg acagatgaag tttccttagg
    12421 agattatgtc cttactgggg gagaattagg tgcgatggtc atgatcgatg caacagttcg
    12481 gcttttgcca gatgtgttag ggaatcagac ttcagctcaa acggattctt attcaaccgg
    12541 tttattggaa catccccaat atactcgccc ggcagaatat aaagggatga aagtaccaga
    12601 agtattgaca aatggaaatc ataaattgat tgaagaatgg caattaaaag aatcactaag
    12661 aaggacctat caacgtcggc ctgatctttt ggaaacactt gaatggacac ctcaaatgac
    12721 gaaattttta gaagaaatca agaaagaaga acaagaaaaa acagcagatg aagccaagtg
    12781 actttttcta aaatcttctt tacagtaggt tgataatatg ttagtatttt atggtgagtg
    12841 caaaagcact taactattac gatattccgc tgtgagaaaa gctcataaga atatttggaa
    12901 taaggagaag aaaaccatga atccattaat cgaagaatta acaaaagaac aattacgttc
    12961 tgacatccca gcgttccgcc ctggtgacac tgtgcgtgtt cacgcaaaag ttgttgaagg
    13021 tactcgtgaa cgtatccagt tatttgaagg tgttgttatc aaacgccgcg gtgctggaat
    13081 cagcgaaact tatacagtac gtaaagtttc taacggagtg ggcgttgaac gtacattccc
    13141 attgcataca ccacgtgttg caaaaatcga agtagttcgc tacggtaaag tacgtcgtgc
    13201 aaaattgtac tacctacgtg cattacacgg aaaagctgct cgtatcaaag aaatccgtcg
    13261 ttaatcattg ttatcagact gagaaagaac ccttggtatt caagggttct tttttcttag
    13321 catgtaattt gaacaccctg atccagtttt ttctttaatt attggtaggg gagaataatg
    13381 gattagaata aatctaccta taagtagtag taaaaaaaag agcactatga taatatcttt
    13441 cttaagagta accgctttaa taaaaaatcc agaatattct aacataaatg gagtattctt
    13501 agaatcaaaa ggagaaagaa gatggtttat acttatacgg agcaagaaaa gatgaaagtt
    13561 ttagacacat tcatgaatga tggaaaagta aaacaagtac caagttcaac aaaaaagaaa
    13621 tacatcctat tgaaagaagt cttgaaacgc tttgaccatg gagtgactta tacagaaaca
    13681 gaggttaatt cgatactgct gaacgtattt tctagtggcg attatgtcga acaaagacgt
    13741 tatctaatca catttggctt tttcaaaaga agttcagacg gtcgggcata tcagatgatg
    13801 ggaattgaaa attagaagtt ttaatttata ctcattgaaa aaagttcaag tatgcgatat
    13861 tggttctaca ccgcatactt gaactttttt tgtcatctgt atctataata caaagccaag
    13921 aacgttattc aaaatatgga tagctatgat aaaagctaat gagcgattcg cagttaaata
    13981 agcaatcgtc agtaaaatcg ttccaggagc gtacatgaaa aaatcaattg gatgcgttac
    14041 atgaataaag ataattctct tttatttgta cgaataaata ccaaaacgta cctcctaaaa
    14101 ttagattttg gacagatgcc ctttaaaaag ataacaagat ttcgttcttg aaacaatata
    14161 tatgctttta ttccaatgag gttgtgaaaa cagtattttg tcctgagcgt tagaaaattg
    14221 aaaatgggga atagcaactc gtactttacc attttttaag tgaatacaag atcagctaat
    14281 tgaacaccgt ttattcgtga tgagggacac aacactgcag tcgacacatt tctcttttat
    14341 gttcaaagat aacaaatatt taaaacaaag aaaaatagta gataatcccg tcctaactct
    14401 tttattcttc cctcatagct tccaaattta agcaatactt caattaatca agttttatct
    14461 ttttttttca ataatcgtaa aaaaagagta ttttttattt aaaaataaaa tctttgtatt
    14521 aaaggacaag actaaaataa ccttagaata aaaagagaaa aa
//
LOCUS       DAFBQH010000071        14153 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.073, whole genome shotgun sequence.
ACCESSION   DAFBQH010000071 DAFBQH010000000
VERSION     DAFBQH010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.073"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            1..300
                     /locus_tag="LK694_002202"
                     /pseudo
     CDS             1..300
                     /locus_tag="LK694_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            431..1381
                     /locus_tag="LK694_002203"
     CDS             431..1381
                     /locus_tag="LK694_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBK5053044.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            1899..2354
                     /locus_tag="LK694_002204"
     CDS             1899..2354
                     /locus_tag="LK694_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK5053045.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2695..3297
                     /locus_tag="LK694_002205"
     CDS             2695..3297
                     /locus_tag="LK694_002205"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053046.1"
                     /translation="MLDPLFTDETFITFGFGHLVELAEPGHYDEKWQNWKLESLPIFP
                     DRYDFEVAKDKGKQFKIVAELLKKANTIIVATDSDREGENIAWSIIHNANAFSKDKTF
                     KRLWINSLEKDVIRSGFQNLQPGMNYYPFYQEAQTRQIADWLIGMNASPLYTLNLQQK
                     GVQGTFSLGRVQTPTLYLIFQRQEAIENFKKEPFFEVEAK"
     gene            3391..3723
                     /locus_tag="LK694_002206"
     CDS             3391..3723
                     /locus_tag="LK694_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HBK5053047.1"
                     /translation="MGNQEGIIADVQTKEKKTNSPSSFSLSSLQSKVNQLYKATASQT
                     LKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIRSN
                     NSTQILTN"
     gene            complement(3874..4853)
                     /locus_tag="LK694_002207"
                     /pseudo
     CDS             complement(3874..4853)
                     /locus_tag="LK694_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(4858..5625)
                     /locus_tag="LK694_002208"
     CDS             complement(4858..5625)
                     /locus_tag="LK694_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK5053048.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6115..6540
                     /gene="lacA"
                     /locus_tag="LK694_002209"
     CDS             6115..6540
                     /gene="lacA"
                     /locus_tag="LK694_002209"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HBK5053049.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6557..7072
                     /gene="lacB"
                     /locus_tag="LK694_002210"
     CDS             6557..7072
                     /gene="lacB"
                     /locus_tag="LK694_002210"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HBK5053050.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7083..8015
                     /gene="lacC"
                     /locus_tag="LK694_002211"
     CDS             7083..8015
                     /gene="lacC"
                     /locus_tag="LK694_002211"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HBK5053051.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8018..8998
                     /gene="lacD"
                     /locus_tag="LK694_002212"
     CDS             8018..8998
                     /gene="lacD"
                     /locus_tag="LK694_002212"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBK5053052.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9026..9343
                     /locus_tag="LK694_002213"
     CDS             9026..9343
                     /locus_tag="LK694_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBK5053053.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9343..11049
                     /locus_tag="LK694_002214"
     CDS             9343..11049
                     /locus_tag="LK694_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK5053054.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11191..12597
                     /gene="lacG"
                     /locus_tag="LK694_002215"
     CDS             11191..12597
                     /gene="lacG"
                     /locus_tag="LK694_002215"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBK5053055.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12620..12892
                     /locus_tag="LK694_002216"
     CDS             12620..12892
                     /locus_tag="LK694_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HBK5053056.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            12913..13812
                     /locus_tag="LK694_002217"
     CDS             12913..13812
                     /locus_tag="LK694_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBK5053057.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4614 a         2349 c         2737 g         4453 t
ORIGIN      
        1 tttttaccga tgatgatatt tttttaattt tagcaatact ttttcttgtt tcatctaatt
       61 ttagtgataa cttaattgaa gttcttacag aaccagaaat tcggtctatg acggatgaag
      121 aacaacagat tattaaagct atgatttcgg aaaataagaa agacgtagta gttatcaaaa
      181 agattagaga gtatacaaat ttaggtgttt tacaagccaa aaaagtatat gaaaaaaaaa
      241 aaaagggtct ataattacaa attagataat acattagaag ctggagacaa gattgtctag
      301 ataatgtgtg agataaaatt tttggcaggt tatcatacat aagacaaaat gtttttaaaa
      361 ctgataacgt atttattcca caacaattac gcatttattt ctgagaaaaa tataaagcag
      421 gaggattaca atgctgaaaa cttattcctt aacaaaagat acattattag tttcgtcaga
      481 atcaaatatc aaaaattctt ctgttgttta tgtttatggt gacgaacgag aatatatcga
      541 atcttctgaa cataaatatg actttaaaat aggcaatata ctaacgtttg atgatcgagt
      601 tgcatatgac acgatgatag ataagaacga tgaaaaaagt ttgctcgtaa gttttttgtt
      661 tcccgaggta catgaaggtg ggcatctaga taccctgacg acatctgttg ttttcttggt
      721 atttaaaaat aagttgataa tttttacaaa acaaaaatta aaattcattc ccgaactctt
      781 atccaatatg gttttacgtg atgtcaatca tttcgtggag gaagtacttt taaagatcat
      841 gcaaaaagat ttctatatga tgcttaacga ccttcgtgga gtcaaggaaa aaattgatga
      901 tttagactca gaaatatcaa cgtccggttc actcaaacca acgtttggtg atttattgac
      961 tcttcaaaaa tacatgatag ctttgtcaac aacttataaa gctaaccata aagcgcttga
     1021 tttcattaaa aaaaatttca caactattga cgatattgat tttagtacta aaaaaataat
     1081 tgatgattta tacgatacaa gtgatacaat ggatagaatc attttaagtt acagccaata
     1141 tttggacaaa ttggaaaata tgataaacaa tatgatttcg tatcagctaa acatgattat
     1201 gaaaacccta accgagatat caattgtttt gactattcca gcaattattt ttggcttttg
     1261 gggaatcaat gtccatgtcc cgtttgaaaa gtcttcttat ggtgttttag ccgtattgat
     1321 tatctctgct atactaagtc taatttgctg gcttttgatg cgacgcaaaa catatttgta
     1381 aagtatttgt gataccctaa tatgatttaa agcagaattt ctttgttgac tttttaaaag
     1441 gctgattcct ttcaataatt taatagcttc agcggctgct cagaagtcaa taaattggac
     1501 tatttttatt cagttactta ggcaacattt gagatacgat tgcgattata aaaattgata
     1561 taccaaccaa tggctgcttg gaagactgac atattagcct cgatatcatc gaataactaa
     1621 atacagaaaa atacaataaa tattatcaaa ctagggacta tactagaaaa catcagtaca
     1681 ttccttaact cagggtctat aattagaaaa tcgccccttt gaaaactgat taaaacagcc
     1741 cccattatct agtgactaga taatgggggc tgtttttttg ttaagtgata taataaacca
     1801 taaaagaagt ttagaaatac ggtcctggtc atcaataaat aaacaattta attaattatt
     1861 tatttaatta tttaatttca gaaaggtata catgaatcat ggtgcaacaa attgtcctac
     1921 ccatcaaaga ctccagcgtc ttaaagatgg tacaagatac acttctcgat agtttccgtg
     1981 ctggtcgccg aaattacacc gtttttcaag ttggtaaggc cacgctacta cgtgtgagtg
     2041 atgtcatgac attaaaaaaa tcagatgtct ataatccaga cggctctgtc aaaaatgcaa
     2101 cctttattca tgataaaaag accggtaaag caaacacgtt atatttaaag cctgttcagc
     2161 aagacttgtt gcaatatcat gattggctgg cccaagaaaa tatcaattct gagtggttat
     2221 ttccctcgac agcccattat gatcttcata tcactgagaa gcagtttcat aaggtgatgg
     2281 cgcatgttgg tgatctctta ggaatcaact tggaagacaa agagaaaaaa ttgcgagtca
     2341 gaaaaccgtt ttgagatatg aacagcattt gccttcacgc cttagcgatt ttaaataata
     2401 gccttgaaag tgattatgga aaagccctct acgctcaatg caaaattgac gtagaaacta
     2461 tttttgatcg attgaaaggt gtatttggta tgtgaagaac ccatgtcaga ggcaagaacg
     2521 ttgcacctac tgacattgga atgatgctta tgagcatgaa tctaaccaaa ttatcccttg
     2581 aggctagaag aaaagcggaa gcttttcaac acaaagcagc caaaaataaa aaacgcgacg
     2641 aagcaatcac atttatgatt attcatcgcg ttttttatat ttagagctag ttatttgcta
     2701 gacccactat ttacagatga aacgtttatc acctttggtt ttgggcattt agtggaatta
     2761 gcagaaccag gtcattatga cgaaaagtgg caaaattgga aacttgaatc tttgccgatt
     2821 tttcctgatc gatacgattt tgaagttgca aaagataagg gaaagcagtt taaaattgtt
     2881 gcagaacttc tcaaaaaggc aaatacaatt attgttgcaa cagatagcga cagagaaggt
     2941 gaaaatatcg cctggtcgat tatccataat gcaaatgcct tttcaaaaga taaaacattt
     3001 aaaagactat ggatcaatag cttagaaaaa gatgtgatcc gaagcggttt tcaaaatttg
     3061 caaccaggaa tgaattacta tcccttttat caagaagcgc aaacacgcca aattgccgat
     3121 tggttgatcg gcatgaacgc aagccctttg tatacgttaa atttacaaca gaagggcgta
     3181 caaggtacat tttcactagg acgtgttcaa acgcccacct tataccttat ttttcagcgc
     3241 caggaagcca tagagaattt taaaaaagaa ccttttttcg aggtggaagc taagtaaaac
     3301 aagggtcatt taagggcgtt ctaagcccca cacagcgttt taaaacccaa gaggagcttt
     3361 ttagcttttg tttcttctaa acaagctaaa ataggcaatc aagaggggat aattgctgat
     3421 gttcaaacca aagagaagaa aacgaatagt ccgagttcgt tttctttaag tagtttgcaa
     3481 tcaaaagtca atcagcttta taaagcgaca gcgagccaaa ctttaaaagc tatgcaagga
     3541 ctgtatgaag caaaattatt gagttatcca agaacagata caccatttat tacagagaac
     3601 gaatttgctt atttaaaagc gaattttggc aaatatagcg gttttttagg acttgatctt
     3661 gaaatgttgt tatcattgat aatgatacgt tcaaacaaca gcacccaaat tttgacgaat
     3721 tagtgaaact ctatgaaaaa gacgtagtaa aacatgcaac atcctattct aatcagctgg
     3781 taaagcttaa ttaggttata taagcttgaa aataaagggt ttgtccaata tggacaagcc
     3841 tttttcttat gttttgccac agatttgcca cacttattgt gtaagagtat caaggatagg
     3901 tataatttga tactttgttt ttttagtgat gtgcaaataa atttctgtta cacgactacc
     3961 tctagaatgc cctacacggt cttgaattgc ttctaggggt attccttact ctgctaagaa
     4021 actaatatgc gtatgacgga atatatgggt cgtaatatgt ttattaaaac taagtttatt
     4081 atcaatatca attcctgctc gttttggatt accttttcga tttaattttc cattttcagg
     4141 tgagttgagg aaattgttaa ggacactata atgaagaggt gttccattat agtcgggttt
     4201 atgacgactt ttggaagtaa aaatccatcc attatcttta taatcaggat tgaatctcat
     4261 atgttggtga tttaactcaa tttcctcttt aagaatttct atagcccttt tagataattg
     4321 gacatttcgg taactttgta agttttttac attaccttca tagctatttt ctgcagctcc
     4381 tccgtgagtg gactgactaa tgatgatatt ttgcttttca aaatattgat aggcaccaat
     4441 attttggagt ttcgactctg taagtcaaca gggccattag ttagaatacc tattgggatg
     4501 cggtttttat gtagagccag cagtagttga tgtatctcag gaatcatcgt aatctgatcg
     4561 agttcatatt catagtcagc ttggaagtct agagcttctg tcctagaaat actatcgatg
     4621 ccaaattctt ccatagtctt ctgacagcga aaaattctaa gctgctcaat actgatgagt
     4681 tttttcgtgt atttaggaaa agcaacatca gaccaatatc tgaaacgttt gtaaatgtta
     4741 tctatttgtt ggatatcaat cgttggaaaa catttctcaa cgctacgttt aaaggggagt
     4801 tgatggtcat aaagagtgtc atcaaggtca aaaacaatat ttttataagt cataattcta
     4861 gtccgctttt aaaatagtag tgtactggct aaattcctct aagtgttgag ggctaatctg
     4921 gttatctgta atgatggtat cgagttgatc tagattatag aagttaaaga aatcgtatcg
     4981 atcgaattta gtactgtcta ctaataagaa tttttctaca gcattgttta gggcaagttg
     5041 ttgaatcaca ccttccttgt cactatatgt agcaatagaa ttatgggtaa cagcatttgc
     5101 actaacaaaa gctttggcaa atctaattgc ttttaaattt gtcgaagcca ttgaacctac
     5161 aaaagctcca gttatttctc tatattcacc gccaagaagc aataaatcaa tggtttcgct
     5221 atcatttaga atcaagaaca cagggagact atttgtaatg acacgaattt tataaccttt
     5281 acgacctttc aattctaatg ccagttgttc aagtgtagtt ccaggtccaa taaaaatagt
     5341 atcgccatct ttgattaaag agcaggcttt tttagcaatg tcaatttttt cttttgtatt
     5401 tagacttttc ttctcgatat gtgtcttttc aagtggcttt ttagaggaaa gcaattgtgc
     5461 accaccatga gtacgtgtta aaagtccatc agcttctaga gcatcaaggt ctctacgggc
     5521 tgtcatatcg gaaatatcta gttcatctat tatttctttt atggttatgg tcccatcaat
     5581 ctttaacata tctaaaattt tttctaatcg tcgttttttg ttcatatgaa gacccttctt
     5641 tcataaagta atttttttcc aaagataatt ctcttttaat tgtatcataa aagacaatat
     5701 tttcaaggta aaacaaacaa tttcaaacaa aaacaaatgt tagatgatga aataagaaca
     5761 gaggattgac gtatattagc ttaggtcaga ttttgtataa gacgaaaata aagtaggacc
     5821 tcttaatcag taagttatag aaagtaaaag acttttgtaa tacttgaata gatatttcac
     5881 gtccattttg tgatggatta aatgaacaaa aatgaacaat aatttaacgg tgttatctat
     5941 tttttaaaaa aacaaataaa aaaaacaaaa aataaacaaa aaaagtgttg ctttttgttt
     6001 gaatgcttga tattatataa acaaagaaat gatgaaaacg ttttttcgaa cattttgcaa
     6061 aatgttttct acttctacgt agcatttctt tctaaaattt aggaggtggt caaaatggct
     6121 attgttgttg gtgcagatct caaaggtacg agattaaaag atgttgtgaa aaattttctt
     6181 gtagaagaag gttttgaggt aattgatgtg actaaggacg gacaagattt tgttgatgtt
     6241 accttggcag ttgcctctga agtaaataaa gatgagcaaa acttaggtat tgtaattgat
     6301 gcttatggag ctggtccatt catggtagca actaaaatta aaggtatggt tgcagcagaa
     6361 gtttcagatg aacgctcagc ttatatgaca cgtggacata acaattcacg aatgattacg
     6421 gttggcgctg aaatcgtcgg tgatgagctt gctaaaaaca tcgctaaagg tttcgtcaat
     6481 ggtaaatatg atggcggacg tcatcaaatc cgagtagata tgcttaacaa aatgtgttaa
     6541 gaaaaggagt taaggaatga gaatcgcaat tggatgtgac cacattgtca cagatgaaaa
     6601 aatggcagta tcagaattct tgaaatcaaa aggacatgag gtccttgatt tcgggacata
     6661 tgaccacgca cgtactcact accctatcta tggtaaaaaa gtcggtgaag cagtagtaag
     6721 tggtcaagca gacttgggag tatgtatctg tggtacaggt gtcggcatta acaatgcagt
     6781 caataaagtt ccaggtgttc gctcagcgtt ggttcgtgat atgacatcag ctatatatgc
     6841 aaaagaagag ttaaatgcga atgttatcgg tttcggtgga aagattactg gtggtcttct
     6901 tatgaatgac atcattgaag ctttcattga agctgagtac aaaccaacag aagaaaataa
     6961 aaaattgatt gcaaaaatcg aacacgttga aacacataat gcacatcaag cagatgaaga
     7021 tttctttaca gaattccttg aaagatggga ccgtggtgac taccacgatt aaggtataac
     7081 aaatgattct gacagtcaca ctaaatcctt cagtagacat ctcttatcct ctagaaacat
     7141 tgaaaattga tacagtgaat agggtgaaag atgttagtaa gacagctggt ggaaaaggct
     7201 taaatgttac tagagtcttg tatgaatctg gtgataaagt tactgctaca ggtttcttag
     7261 gtggtaaaat tggtgaattt attgaaagcg aattagaaca atctcctgtt agtccagctt
     7321 tttataaaat ctcaggaaac actagaaact gtattgctat cttacacgag ggtaaccaaa
     7381 cagaaatttt ggaacaagga ccaacaattt ctcatgaaga agcagaaggc tttcttgacc
     7441 actatagcaa tcttattaaa caatcagaag ttgtgacaat ttcaggaagt ttaccttcag
     7501 gacttcccaa cgattattat gaaaaactta tccaacttgc ttcggatgaa ggagtagcag
     7561 tcgttttgga ttgttcaggt gcacctcttg aaacagttct aaaatcgtcg gctaaaccta
     7621 ctgccatcaa acctaataat gaggaacttt cacagttgct tggaaaagaa gtaacaaaag
     7681 atattgaaga actcaaagac gttcttaaag agtcactttt tagtggtatc gaatggatag
     7741 tggtctcatt aggtcgaaac ggtgcctttg caaaacatgg tgatgttttc tataaggtag
     7801 atattcctga tatcccagtt gttaatccag tcggatcagg ggactcaacg gtagctggta
     7861 ttgcatcagc tttaaatagt aaaaaaagtg acgctgactt attaaaacat gcgatgacat
     7921 tgggtatgtt aaatgctcaa gaaacaatga cagggcatgt gaatatgact aattacgaaa
     7981 cactaaactc acaaattgga gtaaaagagg tataaaaatg gtacttacag aacagaaacg
     8041 caaatctttg gaaaaaattt cagataaaaa cggtgttatc tcagctttgg catttgacca
     8101 acgtggtgct ttgaaacgtt tgatggcaca gtatcaagat acagaaccaa ctgtagctca
     8161 aatggaagaa ctaaaggttt tggttgctga tgaacttaca aaatacgctt cttcaatgtt
     8221 gcttgaccct gaatatggac ttccagcaac aaaagcattg gataaagaag caggtcttct
     8281 tcttgcctat gaaaaaacag gttatgatac atcaagtaca aaacgtttgc ctgactgctt
     8341 ggatgtttgg tctgcaaaac gtatcaaaga gcaaggcgct gatgccgtta agttcttgct
     8401 ttactatgat gtagacagct cagatgaact taaccaacaa aaacaagctt atattgaacg
     8461 tgttggttct gaatgtgtcg ctgaagatat tcctttcttc ttggaaatcc ttgcttatga
     8521 tgaagaaatc tcagatgcag gttctgttga atatgcaaaa gtgaaacctc gcaaagtcat
     8581 tgaagcgatg aaagttttct ctgatccacg ctttaacatt gatgttctta aagtagaagt
     8641 accagttaac gttaaatatg tcgaaggttt tgctgatggt gaagtagttt acagtaaagc
     8701 tgaagctgct gacttcttta aagcacaaga agaagcgact aaccttccat acatctacct
     8761 aagtgcaggt gtatctgcta aattgttcca agaaacattg caatttgctc acgactcagg
     8821 tgccaagttt aatggtgtgc tttgtggacg tgctacttgg gcaggatctg tagaacctta
     8881 catcaaagaa ggtgaaaaag cagcacgtga atggttgcgt actactggat tcgaaaatat
     8941 tgatgaactt aacaaagttc ttgttaaaac agctagtcca tggactgata aagtatagta
     9001 aaaacataaa acggaggata ttgttatgaa cagagaagag atgactctct tagggtttga
     9061 aattgttgct tatgctggag atgctcgctc taaactttta gaagcgctta aagcggctga
     9121 aaatggtgat ttcgctaagg cagatagtct tgtagtagaa gcaggaagct gtattgcaga
     9181 ggctcacaat tctcagacag ctatgttggc tcgagaagct tctggggagg aacttccata
     9241 cagcgttacc atgatgcatg gtcaggacca cttaatgact actgtcttat taaaagatgt
     9301 gattcatcac ctcatcgaac tttataaaag aggagcaaaa taatgcataa actcattgaa
     9361 cttattgaga aagggaaacc gttctttgag aaaatctctc gaaacatcta tcttcgtgct
     9421 attcgtgatg gatttatcgc tggtatgcca gtcatccttt tctcatctat ctttatcctt
     9481 attgcctatg tcccaaatgc ttggggattc cactggtcaa aagacattga gaccttcttg
     9541 atgactcctt atagctattc gatgggtatc ctagcattct ttgttggtgg tactaccgct
     9601 aaagctttga cggactcaat gaaccgtgac ctacctgcga ccaatcagat taacttctta
     9661 tctacgatgc tagcgtctat ggtagggttc cttttgatgg cagctgagcc tgcaaaagaa
     9721 ggaggcttct tgacagcctt cacgggaaca aaaggtcttt tgacagcatt tattgcagcc
     9781 tttgtgacag ttaatgttta caaggtctgt gtgaaaaata atgttactat tcgtatgcct
     9841 gaagaggttc caccaaatat ctctcaagta tttaaagact tgattccatt cactgtagcg
     9901 gttgttctcc tttatggatt tgaactcatt gttaagggaa ctcttggtgt aaccgttgca
     9961 gaatcaatcg gtacccttct tgctcctctc ttctcagctg cagatggtta ccttggaatt
    10021 acacttatct ttggtgctta tgccttcttc tggtttgttg gtatccacgg tccttcaatt
    10081 gtcgaaccag caatcgctgc tatcacttat gccaatatcg atgccaactt gcatcttatc
    10141 caagctggac aacatgcaga caaagttatc acttctggta ctcaaatgtt tattgttacc
    10201 atgggtggaa caggagctac attgattgtt ccattcttgt tcatgtggat ttgtaaatca
    10261 gaacgtaacc gtgccatcgg acgtgcctca gttgttccaa cattctttgg ggttaatgag
    10321 ccaatcttat ttggtgcacc aatcgtattg aatccgattt tctttgtacc gttcattttc
    10381 gctccgattg tcaacgtttg gatctttaaa ttctttgttg acacattgaa catgaactca
    10441 ttctctgcca accttccatg ggttactcct ggtccgttgg gaattgtact cggtactaac
    10501 ttccaagttc tatcatttat cttagccgga ctcttggtag ttgttgatac tatcatttat
    10561 tacccatttg ttaaggtata cgatgaacaa attcttgaag aagaacgttc tggtaaaaca
    10621 aacgatgctc ttaaagaaaa agtagctgca aacttcaata ctgctaaagc agatgccgtt
    10681 cttggaaaag caggcgttgc taaagaagat gtagcagcaa acaataatat cacaaaagaa
    10741 actaatgtcc ttgttctttg tgcaggtgga ggtacaagtg gcttgcttgc caatgccttg
    10801 aacaaagcag cagcagaata caatgttcca gtcaaagcgg cagcgggtag ttacggtgct
    10861 caccgtgaaa tgttgccaga gtttgacttg gttatcttgg ctccacaggt cgcttcaaac
    10921 ttcgatgata tgaaggctga aacggataaa ttgggcatta aacttgcaaa aactgaagga
    10981 gctcaatata tcaaattgac acgtgatgga caaggtgctc ttgcatttgt tcaacaacag
    11041 tttgattaat aaatttgtaa aagttatttg agacgagatt attctttcaa ttccttataa
    11101 tattgttaac catatcattg catttcattt ccttggtttc cggaactcag aatatcgtct
    11161 caaatgcttt ttttgaaagg atttatagtt atgactaaaa cacttcctaa agattttatt
    11221 tttggtggtg ctacagctgc ttatcaagca gaaggtgcga ctcacacgga tggtaaaggc
    11281 ccagttgcat gggataagta tcttgaagac aattattggt acacagcaga gcctgcaagt
    11341 gatttttacc ataaatatcc tgttgattta gaattagctg aaaaatatgg cgttaatggt
    11401 attcgtattt caattgcctg gtctcgtatt ttcccaactg gctatggcga agtaaatgaa
    11461 aaaggtgttg aattctacca taatcttttt gccgagtgcc acaaacgtca tgttgagcca
    11521 ttcgtgactt tgcaccactt tgacacacca gaagttcttc actcagatgg agatttctta
    11581 aaccgcgaaa atatagaaca ctttgtagat tatgccgctt tctgttttga agagttccca
    11641 gaagtaaact actggacaac tttcaatgaa attggcccaa ttggagatgg tcaatacttg
    11701 gttggtaaat tccctccagg cattcaatat gatcttgcca aagtcttcca atcacaccac
    11761 aatatgatgg tttctcatgc acgtgctgtg aaattgtata aagataaagg ttataaaggc
    11821 gaaattggtg tagtacatgc gttgccaacc aaataccctt atgatccaga aaatccagca
    11881 gatgttcgtg ccgctgagtt agaagatatc atccataaca aatttatctt ggatgcaacc
    11941 taccttggac actattcaga taaaacgatg gaaggggtca accacatcct agctgaaaat
    12001 ggtggagaac ttgatcttcg tgatgaagac ttccaagcac ttgaagcagc taaagatttg
    12061 aacgatttcc ttggtatcaa ctactacatg agtgattgga tgcaagcctt tgatggtgag
    12121 actgaaatca ttcacaatgg taagggtgaa aaaggaagtt ctaagtacca gattaaaggt
    12181 gttggtcgcc gaatcgcacc agattatgtg cctagaacgg attgggactg gattatttat
    12241 cctgaaggct tgtatgacca aatcatgcga gtgaaaaatg attatccaaa ctacaagaag
    12301 atttacatca ctgaaaacgg tcttggatac aaagatgagt ttgtagataa taccgtttat
    12361 gatgatggtc gtattgatta cgtgaagaaa cacttggaag ttttatcaga cgcaattgcg
    12421 gatggtgcaa atgttaaagg ttacttcatc tggtcactta tggatgtctt ctcatggtca
    12481 aacggatacg aaaaacgtta tggactattc tatgtagact ttgacacgca agaacgctat
    12541 cctaagaaat cagctcattg gtataagaaa gtagcagaaa ctcaagtgat agagtaatat
    12601 tacttgaaaa tgtaaaaaat tgttatccga tactttagaa aaagtacaac acttatttag
    12661 accaggcttt gaaaaggtat atattgttag tttcgaagat tgtcctatga ttcctgagct
    12721 tgaagctacc ccgttgctaa aatgtggtaa atggtatgtt tcaactggaa aagagtggat
    12781 ttgccattct gatttagagt tatcagcatt tgaatgggaa tttttacaat cactcgatgt
    12841 agaaatacgt gagactattc acttcgaggt aaattatttg cctttccaat aataaaaaga
    12901 aaggaattag ttgtgactat cgaattaaaa aacgagtatc ttactgttca gtttaaaaca
    12961 cttggtggtc aactgacttc gattaaagac aaggatggga tagagtatct ctggcaagct
    13021 gatccaaatt attggaatgg acaggcgcct attctatttc ctatctgtgg tagtttacga
    13081 aatgattggg ctatttatag acctcaagaa agaccctttt ttacaggtct tattcgtaga
    13141 catggttttg ttcgaaaaga agaatttatt ctggaagaag tcaatgataa tagcgtgacc
    13201 tttagtatta aaccaaatgc tgagatgctt gataattacc tatatcagtt tgaactgaga
    13261 gttgtttata ccttgaatgg taaatcaatt agaaccgaat ttcaggtaac gaacttggaa
    13321 atggaaaaaa caatgcctta ctttattgga gcccatccag cattcaatag tcctttagta
    13381 gaaggtgaaa actatgagga ttattatatt gaatttagtg aggttgagtc ttgctcgata
    13441 ccaaagagtt ttcctgagac aggattatta gatttgcaag atcggacacc ttttttggaa
    13501 aatcaaaaaa ttctcgattt ggactactca cttttttccc atgatgccat tacacttgac
    13561 cagctcaagt ctcgtagcgt aacgcttcgg tcaaggaagt ctggtaaagg tcttcgagta
    13621 gattttgatg attttccgaa cttgattctg tggtctacaa ctaataaaag tccattcatt
    13681 gctttggaac cttggagtgg tctctccact tcacttgaag aaggtaattt cttagaagat
    13741 aaaagacagg taacgaaaat ttcacctcaa gaaacttcta gaaaaatcta tgatattact
    13801 atccttaatt aaaatttgtt attatttgag tcttcaataa gatttgaata ctcttacctt
    13861 gtgatgatag attggtcttc tccgtgccag tcttacatcg caaggttttt aagttttttg
    13921 tgggtctatg tcaactgtag tgggtgacga aaagctaata tctaaagaca gtctggtagt
    13981 aaaaaataag attatttact acttcttgat ttaaaataat agtggagcca ttaatttgtg
    14041 gcaaatttaa tttaatattt tgcctcaaat tagccacact ttttatagaa ctaagaggaa
    14101 aaatgaaatt ttcttcctat taatatagtt gtttgaaatc ttaaatatag cgt
//
LOCUS       DAFBQH010000072        14031 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.074, whole genome shotgun sequence.
ACCESSION   DAFBQH010000072 DAFBQH010000000
VERSION     DAFBQH010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.074"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..1350
                     /locus_tag="LK694_002218"
     CDS             <1..1350
                     /locus_tag="LK694_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK5053058.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..2827
                     /locus_tag="LK694_002219"
     CDS             1484..2827
                     /locus_tag="LK694_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK5053059.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2939..4390
                     /locus_tag="LK694_002220"
     CDS             2939..4390
                     /locus_tag="LK694_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBK5053060.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            4739..5371
                     /locus_tag="LK694_002221"
     CDS             4739..5371
                     /locus_tag="LK694_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HBK5053061.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            5958..7397
                     /locus_tag="LK694_002222"
     CDS             5958..7397
                     /locus_tag="LK694_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5053062.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            7692..7910
                     /locus_tag="LK694_002223"
                     /pseudo
     CDS             7692..7910
                     /locus_tag="LK694_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8159..8374
                     /locus_tag="LK694_002224"
     CDS             8159..8374
                     /locus_tag="LK694_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053063.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8591..8847)
                     /locus_tag="LK694_002225"
                     /pseudo
     CDS             complement(8591..8847)
                     /locus_tag="LK694_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9034..9945)
                     /locus_tag="LK694_002226"
     CDS             complement(9034..9945)
                     /locus_tag="LK694_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK5053064.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(9964..10968)
                     /locus_tag="LK694_002227"
     CDS             complement(9964..10968)
                     /locus_tag="LK694_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBK5053065.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(10965..13088)
                     /locus_tag="LK694_002228"
     CDS             complement(10965..13088)
                     /locus_tag="LK694_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK5053066.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(13093..14013)
                     /locus_tag="LK694_002229"
     CDS             complement(13093..14013)
                     /locus_tag="LK694_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK5053067.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         4578 a         2498 c         2634 g         4321 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg ggaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2641 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     2701 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     2761 tcctagtttt agccaaccct ataattttat tttaaaggca gtaacaggat taaacattga
     2821 aacataatcg ttactaaatg aaatcaagaa atatactgta ttactcaatt cattgaaaat
     2881 atttatcata atttttaata gaaaacacta ttaaagagaa aaaagagagg taaatcaaat
     2941 gaaaaaatct ttaaaacttg catcagcagt tatattaatg tcccaactcc ttatagcaac
     3001 acctttccat gtgttggcaa gcgaaaattc aacatacaaa attgcaaaag aaagaaccga
     3061 gacgactgaa atgagtaatt ccgatgtaac agcagagagt agtgaacttt tgacaacttc
     3121 atcaagtgaa agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag
     3181 tagtacgact tcaaatgccg agtcttcaac aacttcatca agtgaaagta gtacgacttc
     3241 aaatgccgag tcttcaacaa cttcgtcaag tgaaagtagt acgatttcaa gtaccgaatc
     3301 ttcaacaact tcaagtagta caagtgaaat aacttcttcg aaagtgacaa aaccaaacca
     3361 tatagaatct tcaaaaaagg aagcgccaaa aacggaagga caaacaaaaa ctttttcgac
     3421 accacagcaa atagaatcaa cagaatcttt ccaatcacaa ccaatactat ctggtaatga
     3481 agaaattcat tttgacaaaa atgaatctgt agaaaatttt attatgaaaa ttggtgagtc
     3541 tgcgagaaaa attggtcaga aatatgattt atatgcttct gttatgattg cacaagcgat
     3601 cttagaatct gccagtggtc aaagccagtt agctcaggca ccgaattaca atttatttgg
     3661 gataaaagga acttataatg gaaactttgt cattatggta acaaacgaag atttgggtaa
     3721 tggaacgctt tatacgaccc aatcaaaatt tagagtgtat gaaaactatg aggaaagctt
     3781 tgaagattat gccaaattat taactaaggg aattagtgga aataaagatt tttatgcagg
     3841 agcattaaaa gctaatagta aaacatatcg agaagcaact aaattcctta caggacggta
     3901 tgcaacagat acacaatact atttgaagct aaatgaatta attaaaacct acgatttaac
     3961 caactatgat aaagaagtta aaggtccaca aatgagtaag actggatacc aagtgcctat
     4021 tcataattat tcaataacca gtttgtttgg aaatcgtgga aatgaatttc accgaggact
     4081 tgatatggca gctacacagg gtgagccgat tcatgcagtt aaagctggta aggttgttaa
     4141 agcagagttt cactactctt ggggaaatta cgtggtgata caacacgaag atggatcaac
     4201 cgcgttatat gctcaccagc aacaatacct agtaaaaact ggagataatg ttttgcaagg
     4261 acaaataatt ggctatgtag gaagtactgg gaatagtact ggcccacacc ttcatttgga
     4321 aatttgcaaa gatagtagtc tttctcagag catgttagtt gatccgaaaa ccttattatt
     4381 cggagactaa aaaaggtaat caatctgaca aagtaacaag aaaatggttg gattcttaga
     4441 cctttgatca ggatattgag actggaattg atatctcgat tacgatgggt ttgacaaata
     4501 aaacaagtcc attatcgaat atcgagtgta ttattgccgt ctttatgctc atattttgag
     4561 caggtttcac tagatgaaaa caatttatct atcttgagga tttcgtgtac gtatcgttca
     4621 gtcttgtatt gtaaattagt cataaaatca gactaagaaa aattggaaat atttttttct
     4681 gatttattaa aagttaattg aaattatatg gtacttcttt cttgggaggg taggtgcagt
     4741 gaaggaaaga tatggtcacc ttactgttgt tgaggagatt actcataatg gatataaaaa
     4801 atatcgttgc ctatgtgact gtgggaatga aacgattgta tatcgatcaa acttactatc
     4861 aggtagaacc aaaagttgtg gatgtggaaa ttataaaaat aggcataaat ataaaaattt
     4921 gacaaataaa acttttggta agttagttgc aatcaagcca actaaactgc gaagtgaagg
     4981 aactgttgtt tgggaatgtc aatgtctgtg tgggagaaaa aagttagtat cggcaaagcg
     5041 acttctaaaa ggtgatgtaa aaagctgtgg atgtttgaaa tcaaaaaagc atcacattga
     5101 aaataaaaaa ttcggattac ttactgcaaa gtatcctttg gacaataact gttcatggaa
     5161 aacaaaatgg gtttgtcatt gtgagtgtgg cagtatgtgt gagataagtt atagtaactt
     5221 attttttgga catactcaaa gttgtggatg tttaaaaagg caagaaacaa ggacatgtgt
     5281 tgaagggaca gtaatagagt gtctaacgtc aaaattaccg aaaaataata caagtggtgt
     5341 taagggagtt tatcgttcta aagagaaata gattgcgtat ataactttaa aaaaaagagg
     5401 tactatttag gatcgtatga ttctattaag agtgcagctc aagctagaaa agatgctgaa
     5461 aataaattgt ttcaaccttt gattgacaaa tatcgaggta atcattaatt attgtggata
     5521 ataattaaca gtgatatata aacatctata atatattatt catactattg atatttagta
     5581 taatcctatg gaattgaata tcatagcaat gtatttgaaa agcaaattgg aaaacaaata
     5641 ggtgcaacac cataaatatg aaaaaattgg gaaaaagtag aatggaaatt tgcttacgtc
     5701 tcacgagata gctcacacta ttaatctgaa gaacaatgaa gtcgactttt agaaattgct
     5761 agaagaaagt aaagcgaatt atcactagtt actatagagt aagttcaaat aaagcatctc
     5821 caacttacat ggttacatga ggatatcaaa tcgaaattac tcaagcttcc tatggctaga
     5881 gtcgcgagaa gttgttgggc agtagacata ggttatattg ttcttagcta cacgatcatt
     5941 agaaaggaaa tcctattatg tatagttatc aagaagatgc aaaagtccaa aaaaatcgct
     6001 ggtggatttt gattgctata tctatgttta ccttcatgtc cacattggat ggcagtattg
     6061 tcaatattgc actacctacc atctcagatg atatgggcat accaatgaat caagcggaat
     6121 gggtagtgtc cgtttatctg atgattgtct gttcttgttt gttgctgttt ggtaaaattg
     6181 gcgatagccg tgggaaaatc aaagtatttc ggattggtac gctgatcttt gtcttgggat
     6241 cgctgttgtg tgggttcaat cattcgctag gtattttatt gttcgcacgg gtggtacaag
     6301 ccattggtgc tagtatgact atggctacaa atgccggaat catcacggaa gtctttccca
     6361 tacatgaacg tggtcgggca ttgggagcaa taggtgcgtt tgtttcatta ggctcgattg
     6421 ctggaccagg tcttggcggc ttgattttgt cccaattttc ttgggcgtac attttttgga
     6481 tcaatgtacc ggtgggaatc gtaacgatgt tgattgctga aaaattctta ccaaaagata
     6541 tcaccatgag cggccataca atcgatggag ggggctttgc tgccattgcg gctacgatta
     6601 tcagcttttt tggagcaatc tttttggggc aagaaagtgg ctttttccat ccagtccccc
     6661 ttgtactgtt cgtaatttca gtgattgcct ttgtcagctt tttaaaagtt gaaaagaaag
     6721 cagcctctcc attgatttct ttcggtattt ttaagaacaa gatttttacc atgagcttga
     6781 ttacagcctt gttgattttt tcttccaact tctttgtcaa tgtagtgatt cctttttatc
     6841 tgcaaaatgc tcgcgggttg agtcctagct atgctgggct gttgatgatg gtctttcctt
     6901 tgatgatggt cgtcggggcg cctttaagcg gctacctgac cgaccgggtt ggcccggaga
     6961 ttttggtatt agtaggcttg ggcttattgt cggtaaccca ggtcatgtat atgtttttgt
     7021 cagtggactc tcctttgtgg ctatacattg tcgctactgc catcatgggt gccggcaatg
     7081 ccttgttcca gtcccccaat aatacgatgg ttatgagctc ggtcactatc gaaaacttgg
     7141 gactggcagg ctcgatgaat tcctttgccc gaaatttggg gatggtcagc gggatttcgc
     7201 tttctacgac ggtcttatat caagcgatgt ctgctaagat gggccagcaa gtaactaact
     7261 atatcgcaaa tcggccggat gtctttttat atggaatgaa gattaccttt ttaggcagct
     7321 ttgttttgtg ttttgttgcc tttttcctga ctatctggcg tatcaaaaaa caaggaggta
     7381 ttcagaaaaa tgtctgaaaa aaccactgtt gaagaatttt tggaggcctg cgtatgggtg
     7441 agtgggattt ccatggatga tggaagatga agtgaacttc taaaagccga aaaactcaag
     7501 tattatcttc aaaatactag tgctcaatga gatgcttgat ttgatcacaa gaagactgcg
     7561 aaagccgctg gcaaccgcaa gctaggtatc ccaccgatgg actacgtttt agaggctact
     7621 ggttatgaat acggagccaa cgctctcatc tgcataagaa tacatcaccc agattatgta
     7681 aagattttcg atacttctgt caaaaaatca ttaagataga taaatgattt ccgtctagtc
     7741 aaattggttc agaatgtaga cacacagatg gtaagaagtc actcacaatt cgagaatgga
     7801 cttgtcctat ttgccgtata catcataatc gaaacgtcaa cgatagtatc aatattttga
     7861 ccaagggcta tgaatacaag cattgcctta gaaaacatca agaaccttag gaacaacggg
     7921 gatagcttgg ttaataagag ataccgctgt ttaggcttgc aagtatactc ttttatcaag
     7981 aattccgtgg ctttagtcat gagaggctta atactatgaa aattttcttt agcatttttg
     8041 atttaggttc cttttctaat tataataaga tgaccactac agtaacctac tgaaatatat
     8101 aaacagccac tgtagtacca ttttcaaagt agtctatgag aggagaatct tcattcttat
     8161 gaacgctgga attgcggata tgaatttaat aaaaaaaacc cttaatgatt ttacttccaa
     8221 ttcaatatca aaaggtactg gtatcaattt aagcacaata aaaaagctga aaagtggtga
     8281 aagatcagtg gaaaagctga atcttttaga tgctataaaa ataacggaat ttgctatgaa
     8341 aaatggaaaa gctgagattg aaatatggag ataattatcg tctagtgaat tacttgccac
     8401 ttagctgaat ttaacgatcc ttggtgcttg aagggggaga ttcttgggaa tagagcatac
     8461 ttgcaagcct atgtctttac taacaacgat atctcttatt aaccaagcta tccccgtagt
     8521 tctaaggctt ttacttttat ctaagcaata atcgtattca aaggaaattt ttgaccctta
     8581 gaaagtctca ttttatcaga cgtgagctta ggcattgctg aattttgtta attcggtttt
     8641 aggttgtgct ttgagcataa tatgaaagta ttctttatca cgatttcatt catctaaggt
     8701 aatgtgatgt gattctgaaa ttctttcaaa ggcagttttt gcataagctg acattacatc
     8761 atcaatgaca tgtttacaat gtttcactac taaaataagg gggtaaccaa gaagaaaaac
     8821 tgaatgatta tttgtatcta attccatggt atatcaacca tttcctatat agactgatta
     8881 tgacatggat taaaataaaa aggcactatg tcttaattgt cggttttcga gttaccgaat
     8941 ggcattcatc acccacctat agaggatggg cgacttctgc ctgaaataag ttacactact
     9001 tataacttct aataaaatta gattgagtca ttattattta acatcaacga ttttccagtt
     9061 tccatttttt tgtaatacta atttgtactg aaagtagcta gtggtttttg atacattatc
     9121 aaggtatttt atctttgtac tcactagaaa attttttccg ttttgtagat aaactggatt
     9181 ctctaactcc gaaaacttta agtcaatatt gattggtcgc atcgcaccat tttcaacata
     9241 atactccaat tctttttcag aagctgatgg ataaagttta aaaaatgttt ctaaaaaatc
     9301 ggtgatttct tccgttgttt tggcatcaat ttctgaatcg ttttctactt gttttgcttc
     9361 atattttgcc ttgtctggct ttccaatcat tgttggcaaa ctagttacta aatgattcct
     9421 gttttcatct tcaaagattg tcactcgata tgcagaagaa attgattgcg tcttttttcc
     9481 ctctgtaata tgttgctcca ctaaaaatga aacgacaaat tcttccgagt gtttttcaac
     9541 atctagaatt tttacagatt gaacttcgga actcgtggga atatctgctc gaaccatatc
     9601 ttggattaaa gcgagaccgt tttctgtaag gtactgttcg agattactca ttctcttttc
     9661 aattgcttct ttgttatttt tccatgaaaa atattctttc gcaaaatcct ctgtaaaact
     9721 ttcaacgcca gaagtattca ctaccttctc ttttataacc actttttcat gagttgtatg
     9781 ttgatcaatg gcggtgaaat ttttataaac gccaaaagca agactacaag acaataaaag
     9841 ccaaagggca agcaccattt ttcgatgctt acccacggat acaactcgtt cttttttctt
     9901 ttttgggctc ttttcctttt gattccgttc aatttttatc ttcattcttt ttcctctttt
     9961 ctcttatttg tatcggccag ctccgataag atgctgccgc caatagtttt ccgttagatt
    10021 cgtccacccc actggatctc ctgcatgaaa catgcgattc tcccctgcgt aaatgcccac
    10081 atgggtcaca taagtaccgg catcataggt tgaatgaaag aaaactaaat cacctggttt
    10141 ggcatctttt agatcaatat gagtcatggc atcatattga tcctgagcca cccgaggaag
    10201 tttcaggtca actttggcat aacaccattg ggtcaagcca ctacaatcaa accccgtttc
    10261 tggacttgag ccaccaaaca cataggccgt tccttcatac ttaaatgcct catcaaaaat
    10321 ttcttggact attttgtcat caaactgagt tgtagttagg tattgcttca ctaataaaca
    10381 gtaaaacata ttgccataat tataccgcca cccaccattt tccttaatcg cgattgggtt
    10441 ggggtaatcg actttttgac cacctgattt ttcctttgaa aaagattcag ctaaagcaaa
    10501 actatgcttc ttcccgtttt tcccgacatg atctagatac ccgccaccat agttataggc
    10561 ctgaataata ctatcttgat cacaccctag ttctttggaa cgcttaacca aagacgcaaa
    10621 atagagacag ccttgtttga tggatgcctc ggtacttaat gaattaggtg gtaatccggc
    10681 tgactcagaa ctttgcataa cgtcttccat tgtgccacca gattccactt gaataatcgc
    10741 caaaagaatt ggaatatact ctgttatatc cgattctttg gcatactttt ccaccattgc
    10801 acggtgtttc aatacttctt ctgagacact agttccgcca ggagaagtgc cagaactacc
    10861 atttccctca ctgtcttcac caaacaacaa acccacacac atcaaaatac ccataaaaga
    10921 gatggtcaga aaagttaatg aaataacacc cactttcttc aagttcattt tttctcacct
    10981 gaccttgatt ttggacgatg gttcattctt ttttgtactt tttgattggt ctgctttggc
    11041 agagtggacc tctgtttcac ttgttggaaa gaatttttct tacttgctga ttggcttttc
    11101 ccttttgaaa tgatttcttg actagttagt tttcgtttga tttcgggatt cagatttgct
    11161 ctcacaatcc gctttggtgc ttcttttttc agcatcatat ctttctgagg ttgagctgct
    11221 ttatctattg gaatattcgc ccttcttttt ccctgacgat ttgttgcttc actcagtatc
    11281 ttgcgttttt cgtccatttt cgcatttctt cgttgtcgtt gtttttctcg caaatccact
    11341 cgatccgctt tttcttggaa cagtccgcgc ttgaattctt ctggtgcttt tttcgacttt
    11401 tcgattcctt tatggagatt gtatttcaaa ttcgttggcg tattccttac ctgatccttt
    11461 acttgtttag tcttatcttt caccaaagct tgggtatcca aaactttgcc gatttttctc
    11521 ccagccaaat tcattcggga ttgtttctta ttatttgaaa ctggattctc cttagttaag
    11581 tcagatagta tttcgtgatc gttcggtaat cgtgaattct ttcgtagaga agaaccggat
    11641 ggcttaaatt tggattttga ttgttccttt gccaacttat ttccgactgc tgcaccacca
    11701 ccaaaagcca aagctcgtcc cacatttcga ttgagttgcc gcaattttcg attcattaac
    11761 atttgcggtt ttcgcatgac gcgtcttccc attgactggc tatcattact ctggaggttg
    11821 aacatactca taatgtcgcc caatttgaaa tagataccgg caaaggtcac aatttgcaga
    11881 aaagcaacca tgaaaaatgg ataggttgcc gaaatattga aaaacatcgt ggagatgcta
    11941 aatgcagtcg taatcaccaa agtaacaccc gctcgcatca taatcgtatt gaataaacga
    12001 ataatcgcct tctttcctaa actttcataa gtcggcaaca tggacaataa gaaactaatt
    12061 ggcagaaaca tcgcaaagat aatgaagaga atttgcgaga acaacataat ccctgttaga
    12121 agaaaaacaa agaaggaaat gccaatattg aacaatacca aaaagaccac ggtccctaaa
    12181 cggttcatgg tcttggtaat actgagattc gcattgtcat tgtcttcaat ctctgccttg
    12241 accgcttctt cccgatcttt gcctttgttt tcatctggac tcacagatag aatcttgttg
    12301 acccggtctt caccaatttc ttctatgttg gaatcatcaa attgcagtag taaccaaggt
    12361 tgttggactt gaatcgaaaa caaactatcc cgaatcaaat ccacactatc ttttccttgg
    12421 ctttctgaat tgggtacaac gatctcggtt ccaagggtaa gggccgcttc actaacatct
    12481 gagctaaagt cattgatttt tgtgatatag gtaggcgcat aagcaatgaa cgaacccgat
    12541 agtagaaaaa tcatcacaaa attaatcacg gctcgaactg ctttcgtggt ttctcgtttt
    12601 aataatccgg tatacgccac atacaccccc agagctaaaa tcatcaataa tagaaaccca
    12661 aaataaaatc cggaggtctg gagtccattt tcagtaatac cagccaatgt ttggatattc
    12721 tttccaatac tttcagccgt atccgaaatg aaatctaatt tgtaagcctc ttgaaccaca
    12781 taacccgtgg cgttcgacaa atacaaactc accgtccaaa tgaaattagt aatggcataa
    12841 agaccataca tcacgctttt ccctaagcca tcaccccaat tccaaggcaa ccaatcccaa
    12901 gagctatcca caaagaaatc tagttgatag ttgttcagtg aatatttgga atatagattt
    12961 cctgcttcaa ctgtatcatc aactagccct gtcgcctcgg ctaccgtacc aactaaactc
    13021 aatagaacta tcagagtgag tatgctaata aaaacgattc ccacaatccg aaaaagtttc
    13081 tttttcatcc cctcactcca ttctctcttg aacaggtgga cgggtatcaa aggcatggaa
    13141 gagttcttca aagactggat gtacctgaac cacgcctacc cgaccataca aatcctgcat
    13201 caagcattgg ccattttcca aatctcgtag gcgtttttga ttgctttcat cttctcgatc
    13261 caagccaaag aattccaagg ttttcttgat ttcatgaata tcggtactgc gaaacgcaaa
    13321 cttcaacccg atattattct tcaagcgttc atccagtaaa tcatcactat tttgcgtgac
    13381 aaagtaaacc ccggcattca tagcccgccc agcacgaacg agcttattcg ataatgtttt
    13441 cccttgcgct acttgaagaa aactccacgc ctcatctaag tctacaattt tgaagatttc
    13501 tcgattacta tggataaagt ccaaggcaaa agtggaaatg acgattagca tggaaacact
    13561 aagcaattcc atggtggtat attcttcaaa agtggtttct gcatctggta aaaccaaatc
    13621 cgctacttga atgatattta actgcctatc taaactaatc gactgggtaa tcgaaccatc
    13681 tgagaaaagc aaatgggcaa aatcataatc ggtaaaggac tcgatgtgat ccgcgatatt
    13741 ttccgcaact ggtgagcctt cttttcgaag ctcattaatt acttgtaata atccccgttg
    13801 attgctttgg gtaaccgtcc gaatggcctt acgcaacact ggaaacttct ccccatctcg
    13861 actagaaatt cctgttaaaa aggtcagaat atcaattgct agactttcag aatccttctt
    13921 tttctccata atcacataag gatcaagtaa tccttgattt tttggcttac tagttaagtt
    13981 gagaatattg atttcatccg caatttctgg caaggtttct ttccagccac c
//
LOCUS       DAFBQH010000073        13977 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.075, whole genome shotgun sequence.
ACCESSION   DAFBQH010000073 DAFBQH010000000
VERSION     DAFBQH010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.075"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="LK694_002230"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="LK694_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBK5053068.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="LK694_002231"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="LK694_002231"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBK5053069.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="LK694_002232"
     CDS             complement(2169..2906)
                     /locus_tag="LK694_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBK5053070.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="LK694_002233"
     CDS             complement(2997..3524)
                     /locus_tag="LK694_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053071.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="LK694_002234"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="LK694_002234"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBK5053072.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="LK694_002235"
     CDS             complement(4401..4886)
                     /locus_tag="LK694_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBK5053073.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="LK694_002236"
     CDS             complement(5039..5236)
                     /locus_tag="LK694_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053074.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="LK694_002237"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="LK694_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBK5053075.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="LK694_002238"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="LK694_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HBK5053076.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="LK694_002239"
     CDS             9211..9423
                     /locus_tag="LK694_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBK5053077.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="LK694_002240"
     CDS             9425..11104
                     /locus_tag="LK694_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBK5053078.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="LK694_002241"
     CDS             complement(11358..11558)
                     /locus_tag="LK694_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK5053079.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="LK694_002242"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="LK694_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(12249..12322)
                     /locus_tag="LK694_002243"
     tRNA            complement(12249..12322)
                     /locus_tag="LK694_002243"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12286..12288),aa:Ile,seq:cat)
     gene            complement(12634..13032)
                     /locus_tag="LK694_002244"
     CDS             complement(12634..13032)
                     /locus_tag="LK694_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053080.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
     gene            complement(13025..13477)
                     /locus_tag="LK694_002245"
     CDS             complement(13025..13477)
                     /locus_tag="LK694_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053081.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            complement(13464..13970)
                     /locus_tag="LK694_002246"
     CDS             complement(13464..13970)
                     /locus_tag="LK694_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053082.1"
                     /translation="MDIKWLVQQNDSNLELAIKYLEETIFEDEHLTDNFLQVLKYLEI
                     YSVKKNKLIGENDSPIKTPIELSLRNRMGILQRSEIVKELFYHKFSYEIRLDDTYEHY
                     RIVFFVYNSIEDATATTALTFGFTKNGTINSDKTRQAATESDDICKKVCNGEENYWIG
                     EEKLNEIY"
BASE COUNT         4116 a         2943 c         2348 g         4570 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgacatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaattatg taccctgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc
    12301 tgaccaactg agctaagagt acaggttgtt gccacataaa gccataaaca atcaaccagt
    12361 agaaggtgtg gcaacaatcc tgttatcgca tagcttggag tgtgactatt atgggtgata
    12421 gtgaagatat gcgataacat cactatttta tcgaatgatt tttatagttg tcaatacagt
    12481 catctaaata cttcatatca atcatttatt cagaaacaaa ctatcttgca ggatctatac
    12541 gagttaatta cagagcaaaa aagcctcaac caaggatgac tggttgaggt ctttgtcttg
    12601 caaatttaaa tagataactc tattacttgt attttatttt tgttgactat atagctgata
    12661 taaatcaata actgtaggca taactcctac ttcgctatat aaattagaaa tcaaattaga
    12721 agctttattc aggataggtt tagctagctc tcccatagat tcttctacta attttttata
    12781 tggctgttca tttgtagaaa cacgaataac tgaaaagagt aaaatatcta ccggtgctcc
    12841 ttccatcccc cctacaatgt gagctcgtaa agaaacaagt tttttttcta gatactctgc
    12901 ttcgtcttct ttatatccaa gttcaatatt aaactctaaa tctccataac taaaattatc
    12961 agttaattca aaatgaattt tattcaaact aatattttca agtgcaaaag taacactatt
    13021 tcctttatcc atgtacatat acctcttttt ctacaatata gcggctcgta tcttggtaat
    13081 taacactagc atagaccata tttgacgata cttcttttct ctttctactt ttaaatactg
    13141 agtaactttt attccgaata taaggtttat tttcaatacg gtcatattta atagggccgc
    13201 cagcaacctt aatagtgaaa ttcattgaat ctttatagga caaattttca acttgtttca
    13261 aaagtttatt gtactctgca acaggaatat gcatatctcc gaactcaaat tcaagatatt
    13321 ttttttcact tattcctaac atttttgaga attctgtcac gtttttttgt ttttttaatc
    13381 ttattttaag taattgtttg gacaggtttc tttgttcaga atttaatttt ttatataaat
    13441 caggatgttt ttttaataaa tcattaatag atttcattca acttttcctc ccctatccag
    13501 taattctctt ccccattgca tactttctta catatatcat cactttctgt agcagcttga
    13561 cgggttttat ccgaattgat tgttccattt ttcgtaaaac cgaatgttaa tgcagtagtc
    13621 gcagtagcat cttctataga attgtatacg aaaaaaacaa ttctataatg ctcataagta
    13681 tcatctagtc ttatttcata actaaattta tgataaaaaa gttcttttac tatttccgat
    13741 ctttggagta ttcccatccg atttcttaag cttagctcaa taggtgtttt tattggagaa
    13801 tcattttcac caattaattt atttttttta acagagtaaa tttcaagata ttttaatact
    13861 tgtaaaaaat tatctgtaag atgttcatct tcaaagatag tttcttcgag atatttaatc
    13921 gccaattcta aattgctatc attttgttga accaaccact taatgtccat ctcacta
//
LOCUS       DAFBQH010000074        13973 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.076, whole genome shotgun sequence.
ACCESSION   DAFBQH010000074 DAFBQH010000000
VERSION     DAFBQH010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.076"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            83..970
                     /locus_tag="LK694_002247"
     CDS             83..970
                     /locus_tag="LK694_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBK5053083.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTITSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="LK694_002248"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="LK694_002248"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBK5053084.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="LK694_002249"
     CDS             2272..2718
                     /locus_tag="LK694_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBK5053085.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="LK694_002250"
     CDS             2991..4343
                     /locus_tag="LK694_002250"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HBK5053086.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="LK694_002251"
     CDS             4514..4801
                     /locus_tag="LK694_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053087.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5027..5470
                     /locus_tag="LK694_002252"
     CDS             5027..5470
                     /locus_tag="LK694_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK5053088.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5476..6660)
                     /locus_tag="LK694_002253"
     CDS             complement(5476..6660)
                     /locus_tag="LK694_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HBK5053089.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6928..7626
                     /locus_tag="LK694_002254"
     CDS             6928..7626
                     /locus_tag="LK694_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBK5053090.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7633..8310
                     /gene="nth"
                     /locus_tag="LK694_002255"
     CDS             7633..8310
                     /gene="nth"
                     /locus_tag="LK694_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBK5053091.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8436..8969)
                     /locus_tag="LK694_002256"
     CDS             complement(8436..8969)
                     /locus_tag="LK694_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053092.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9266..11647)
                     /locus_tag="LK694_002257"
     CDS             complement(9266..11647)
                     /locus_tag="LK694_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBK5053093.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11637..12284)
                     /gene="recU"
                     /locus_tag="LK694_002258"
     CDS             complement(11637..12284)
                     /gene="recU"
                     /locus_tag="LK694_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBK5053094.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12348..12887
                     /locus_tag="LK694_002259"
     CDS             12348..12887
                     /locus_tag="LK694_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HBK5053095.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12977..13396
                     /gene="gpsB"
                     /locus_tag="LK694_002260"
     CDS             12977..13396
                     /gene="gpsB"
                     /locus_tag="LK694_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBK5053096.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13418..13785
                     /gene="rnpB"
                     /locus_tag="LK694_002261"
     ncRNA           13418..13785
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="LK694_002261"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
BASE COUNT         4702 a         2400 c         2868 g         4003 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaat cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa
     4981 taggaaacaa aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca
     5041 actgctaata gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga
     5101 aatggaaaaa gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg
     5161 gaaaatccct gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc
     5221 catcagtaag ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga
     5281 agagaataaa aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag
     5341 acacgagcaa ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc
     5401 aaaagaagaa tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag
     5461 tagcaaatga agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa
     5521 tccgaatacc gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc
     5581 gatgacgcgc gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt
     5641 atctgagaca gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa
     5701 accataacag ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt
     5761 gatgaataga taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga
     5821 aaattttttg agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat
     5881 caaagagaag cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg
     5941 agaaaagata gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa
     6001 aggaataacg gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga
     6061 gaaacttaaa tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc
     6121 ataaacggca tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa
     6181 ctcagctaat agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc
     6241 catccatcca aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata
     6301 taaggtggag atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca
     6361 tatcgaattt cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat
     6421 aaggatcgga tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa
     6481 ggcagctaga gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga
     6541 gtgagaataa ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc
     6601 agtgaataag acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat
     6661 gtaaaaactc ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt
     6721 gtttccttgt caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag
     6781 tgaagatacg catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg
     6841 accgatcagt gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt
     6901 atactgtgat tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga
     6961 caaacagtca tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa
     7021 gaatttttat ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat
     7081 ttgcagatga ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat
     7141 cgtctcgtaa caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca
     7201 gatcgatata atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa
     7261 aaaaaatacg aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa
     7321 caagaatttg gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa
     7381 gaagaccatt atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag
     7441 gcttatagtt ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt
     7501 acaaaacaac aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa
     7561 gtagaacagt cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag
     7621 gagtagcgga atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga
     7681 atgtttcccg aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca
     7741 gtgatcttaa gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt
     7801 gcctcttttc cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa
     7861 ataaaaacga ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag
     7921 ctgattgaac gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct
     7981 ggtgtaggca gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc
     8041 gcagtagata cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct
     8101 acggtcatgg aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag
     8161 acacatcata cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc
     8221 gaggtatgtc ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa
     8281 aaggcgctaa aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta
     8341 tgaattatct tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt
     8401 aaatgtacat actcagtagc taggatggta acttattaca atttattgcg ataaaaataa
     8461 aagttgttat ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct
     8521 ataaaataag tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt
     8581 gtaataaaaa tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg
     8641 gatttgggcg gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa
     8701 aaagtgagaa tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt
     8761 aaaagtacag aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat
     8821 aataaacaaa atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa
     8881 aaaagatggt gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag
     8941 ccttctttca aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg
     9001 ggattattaa gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata
     9061 aacaagtaaa tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta
     9121 agctaaatgt tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac
     9181 agcttggtac atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac
     9241 gcttgtagac gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg
     9301 gtgtatggtc atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag
     9361 gagcagatga agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag
     9421 acgctggctc gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc
     9481 tttgactagt agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct
     9541 ggacatagct ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat
     9601 attgatcttt gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt
     9661 ctgtatttgt cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta
     9721 cgtgtgacga ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa
     9781 tcgaataatt tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac
     9841 caagttttgc atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata
     9901 acccagcgat ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg
     9961 tgatcatata agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt
    10021 ctgttccgtc ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat
    10081 tggcgaaagc cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat
    10141 cttgttcttc cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc
    10201 caagattttt taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg
    10261 ctggaacatt acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat
    10321 tatcccagtt tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag
    10381 tttttccagt agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca
    10441 tcgtagaacc aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt
    10501 ctgcgatgtg acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg
    10561 tggaggcaac ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat
    10621 catatagttt cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt
    10681 agacattctt atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga
    10741 cgatcttagt attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat
    10801 taacagcttg atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa
    10861 cagtatctcg tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg
    10921 cttgaggcat cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac
    10981 caaagtacat ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat
    11041 ttacgtagta ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc
    11101 acgcttcttg ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa
    11161 gtttgatcaa ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag
    11221 agagggcaga accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat
    11281 cttctaccga cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt
    11341 cacgcttttc tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag
    11401 atacggtagc gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga
    11461 aaagtcctac acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct
    11521 tgataaataa tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct
    11581 tcttcacact ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat
    11641 ttgccattct tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc
    11701 aaataaggaa tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa
    11761 gataaaggta gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga
    11821 acactattca aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc
    11881 ccattttgtt cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag
    11941 ggaaaagatg ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag
    12001 acgccattat agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca
    12061 cttctcttag gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc
    12121 gccaacccgc gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc
    12181 ccccgattgc cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag
    12241 gcattcttat aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct
    12301 aggccacatt atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa
    12361 aagtaatcta tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc
    12421 ctaaaatcca agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag
    12481 ggctggaatg ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag
    12541 tagcggaatt gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg
    12601 attttggtaa taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag
    12661 cagattatgt cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc
    12721 acactcgttt tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc
    12781 ctggaaaaac gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg
    12841 aaattcggct gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt
    12901 tgatttatca acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa
    12961 aactagaggt gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga
    13021 attcaaaaca aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat
    13081 catcaaagat tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg
    13141 attgagtgca aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca
    13201 acaaacagag gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc
    13261 aaatcttgaa cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc
    13321 agcacaacct aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa
    13381 aacacgtcaa ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga
    13441 cttgatcagg ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc
    13501 ttagcgaaac cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct
    13561 tcggctatgc ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat
    13621 ccggtggaac gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt
    13681 gtctggaaga agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg
    13741 ttttttctcc ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag
    13801 ggaattaggt cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta
    13861 tttttcaata cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta
    13921 ttcggaattt gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttt
//
LOCUS       DAFBQH010000075        13836 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.077, whole genome shotgun sequence.
ACCESSION   DAFBQH010000075 DAFBQH010000000
VERSION     DAFBQH010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.077"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(24..1043)
                     /locus_tag="LK694_002262"
     CDS             complement(24..1043)
                     /locus_tag="LK694_002262"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBK5053097.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(1676..2680)
                     /locus_tag="LK694_002263"
     CDS             complement(1676..2680)
                     /locus_tag="LK694_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK5053098.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(2693..3742)
                     /locus_tag="LK694_002264"
     CDS             complement(2693..3742)
                     /locus_tag="LK694_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK5053099.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(3759..4580)
                     /locus_tag="LK694_002265"
     CDS             complement(3759..4580)
                     /locus_tag="LK694_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK5053100.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(4567..5346)
                     /locus_tag="LK694_002266"
     CDS             complement(4567..5346)
                     /locus_tag="LK694_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5053101.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(5363..5833)
                     /locus_tag="LK694_002267"
     CDS             complement(5363..5833)
                     /locus_tag="LK694_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5053102.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(5845..6264)
                     /locus_tag="LK694_002268"
     CDS             complement(5845..6264)
                     /locus_tag="LK694_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBK5053103.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(6345..9122)
                     /locus_tag="LK694_002269"
     CDS             complement(6345..9122)
                     /locus_tag="LK694_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK5053104.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            9313..9537
                     /locus_tag="LK694_002270"
     CDS             9313..9537
                     /locus_tag="LK694_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053105.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            10316..12031
                     /locus_tag="LK694_002271"
     CDS             10316..12031
                     /locus_tag="LK694_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5053106.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            12034..13797
                     /locus_tag="LK694_002272"
     CDS             12034..13797
                     /locus_tag="LK694_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5053107.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         4065 a         2831 c         2402 g         4538 t
ORIGIN      
        1 acgattaact attgatcatc tatttaacga agagaaaatt cattctccat ttttccgtca
       61 cgtaacatga tttcttttgc tgcttttttg atatcttctc cattatacaa aacattatag
      121 attgtttgag taattggcat ctcaacacct agctgttgag ctagctctac tgctgctttt
      181 gtcgttgcca caccctcaac gaccattccc atgttttcta gaacttcttc cagcttatgt
      241 ccctgtccta acaagttacc ggcacgccaa ttgcgagaat gaacacttgt acaagtaacg
      301 atcaagtccc ccacaccact caaaccgata aatgtcagag gattggcacc catcgcgaca
      361 ccaagacggc tgatttctgc caatcctcga gtcatgattg ctgcttttgc atcatctccg
      421 aatcctaatc catggatagc gccggcacca attgcaatga tatttttcag tgctgcacca
      481 gtttctacgc caatcacatc attatttgta taaatcctga aataatcatt catgaacagt
      541 ttttgtacat aggctgctgc tttttgatca atacttgcag ctgtaattgt tgtgatatca
      601 tgtactgcca cttcctcagc atggctagga ccagataata caacaattgc ctgtcttttt
      661 tcttctggaa tttcttcagc caaaacttct gagattcgtt tatgcgtatc ttgttctagt
      721 cctttacttg catgtataat gattggctta gtattcattt taccaataag ttcctgtgcg
      781 actgaacgaa tcgcttttgt tggaacaaca aataaaatag catctgcgtt atccactgct
      841 tctgccaatt ctttatatcc gataatcgat tctgggatcg tcaaatcagg taaaaaatgc
      901 tgatttgtat gatacttatt gatttcatca atctgttcag gtacatttcc ccaaatacgg
      961 acatcatgcc cgttttctgc caatgtttgt gctaaagcag tgccccaaga tccaggacca
     1021 agaactgcga ttttttgttt catggatatt cctccaaata aataattgat aatttccatt
     1081 atagtataca tttcttaaag ttgggactaa tttccattgt ttttaagtgt ttgtttactt
     1141 cttttatcgt ttttacttga aataatcaat gatatgttac aatagtctac attttatttt
     1201 tacactcgtt atgctttgct tgttcctaag aggaagcagt tttgacaatg ttactaccag
     1261 cattactatt aggaggttag gaaacttctc ctagtgagta ttgatttaaa atacataaga
     1321 gacagattga acatttctaa atctgtcagg tcctgcaacg gacagattgt ctctgttgac
     1381 ggttgacata ggaacttttg ttttagtcaa cattctggat acaaaacgct atgtaaactg
     1441 gcgcaactcg ttgtacttgg acttttacta cggatccatg caacaactaa atagtactat
     1501 tgcataacaa aaaatggttg gcacccacat gtcccattaa acagattata agttgaactg
     1561 tagataatgc aaaaaatgtg ttttataacg taattgatgc caatatttta aaaaaataaa
     1621 gggagcagtt atcgctgctc tctttatttt ttatattctt tttatccagt ctatcctaat
     1681 aatcaaactg acgatagtag cggcgtatct ctaatggatg gcagttcaac tgctccacat
     1741 gagcatcgat gcgttgtgtc actacatgca tcaataagaa agacaaaaca cgtccgcgat
     1801 atttttcact gataccttct actggatagt cttttgtgtc tatcaatgta taattgctgc
     1861 aaatttttgg caaaagttta gccacacgtt cagataacac tctctgttca tcttcgccta
     1921 aaaataatgt gactggggtt gtttcttcta cgatctctaa cgtcccgtga agaaactcgg
     1981 cagcatggat agatttcgat ggaagccagc tttgttcttc ccagtaacac atggcatatg
     2041 aataaacagc tccccattga ttccctgctc caataaaata atgcatacta tcacgtcgat
     2101 gtttttctgc aaatgataag ccaaatgcat ctgctttttt ctctgcttcc actaaccctg
     2161 ttggtaaata acgttcgagc tgttcatagt attctgaata agcttcgaat tctccattca
     2221 aatacatcaa tcgatctgct gccataaaaa atttgatttg ttctgttcca ggagctggat
     2281 aagtgaccac aaaatcgcat agctgagaca acttactgtt cgctttatcg ataaatccaa
     2341 tcagtgtcgc tcctactttc ttgatttgtt ctactgcttt cactacttct tgagttgtac
     2401 cggtaacaga ggacaaaata acgatagaat cttttgttaa tcttttgttt ccagtagtgt
     2461 aatattctgc agcatgctgt acaaagacag gcaagttgct tttcccattc atataagtga
     2521 cagcttgcat cgaggaagca tacgttccac cgattcctag ataatagaca gcgtcaaatt
     2581 tttccgcata aagtttatcg atgatttctt ctacttgcgg acgtaaagct agtgcgcctt
     2641 ttattgcttt gatttgttct tcttcattga atttcagcat gatttccacc tcttataatt
     2701 tacttttcaa gactgtatca aagtcttcaa aaatcttttt gttcaagtca atacctttag
     2761 ctactgcggt tttataagcg aatagttgaa acggaacgac aagcgctaat acagacagat
     2821 attcatcaca ttctattgct aatccaagtg ttttctcgtt tcttgctttt tttgtggtaa
     2881 tggtgagagt ctctccaacg taccgactca tatatctttg caacttttgc gaacgttcct
     2941 tatttacact ttccccttca ataaagaata gcaaatgcga agcatctgcc tccaggtatg
     3001 ggccgtgcat ataagcttct aactcaaatc cttgtgacgg aacgcggatt gtttcagtca
     3061 atttcgtctc tgcttcttta gctgttcccc agtttggacc atatccaatg gtgacgaaac
     3121 gctgtgctaa acgaaataaa ctttgatatc catcaaaaaa ttcttccgtt ttttgaataa
     3181 tcgctggtaa atggttgatt attttgcgca actgttgcat gtagtcttgt tctatttttt
     3241 tgctgatttt atttttactt attccaattc ctatccctaa gagtagtaac tgtaaaacag
     3301 tcgcagaata gccttttgta acgaagccta ctgattctac cccggcacct aaatcaataa
     3361 tttgtgcagc ttctaaagca agtggacttt gcacgtcatt tgtgatagca acaacaggta
     3421 acccttgttt ttgtatcatt tttactgctt caacagtaga agcacttttc ccgctttgtg
     3481 acacagcaat cacagtatcg attgaaggag aaagttttcc ataattgaaa aaataaaaag
     3541 gttcttgaat atcgacagta agatcaccat aagattccaa tgctggcttc gctgctaaac
     3601 aagcgttata agatgaaccc gttgctaaga tcagtaaatt cactgttttt ttcatatgtt
     3661 ctcttgtcga aaaatcattc tttttcaaga tcgtacttag tacttccttt tcttcataga
     3721 tataatcttg catcgtaaac attttctctg ctcctttttt aaaaaatccc tatccacgaa
     3781 ccaaaaattc caaagaaagc tagtccgatc aagatcgtta atggtttcac ttctttcttc
     3841 aatagataaa acactatgcc aaatgctcca agacataaga tatttggaac gatatcatca
     3901 aaaatacttt gaacagtcac agcattttct cctgaaccga tttttaaagg aatattgatg
     3961 gtgatcatcg tggcagtcat tgctccaacg accatcagtc caataataga cgcgcctaat
     4021 gtcaaacttt ccatcatacc gtttgcctgg atctttttca aaaaacccgt tcctaagcga
     4081 tatccccaac ctgttgctag atagcgaaac agaatgtgcg gaatattgaa aatcaaaatg
     4141 aacaagatag ggcctaaaat atttccttgc atggctaaag aggtacccac acctgtggca
     4201 atcaatcgca aagttcccca aaagaaagaa tctcctaacc ctgcaagagg tcccatcaaa
     4261 gatgttttga tactatcgat cgcagtcaca tcaaattcag gatcatcctt attttcttcc
     4321 tccattgcag ccgtgattcc taaaatcagt gtaacgatat gtggggtcgt gttaaaaaac
     4381 tctagatgcc gtttcaaagc tgctgccatt tcttctttcg actgatataa ctttttcaaa
     4441 atcggaatca ttgcataaac ataagccata ttcatctgac gttcataatt ccaagaaaac
     4501 tccatttgaa aagaacgcca aaatacgtga tttagctctt ttttcgttat tttgctgttt
     4561 ttgatctcag aattcttcat cttcttctac ctcccctgca gttgtttgaa gtggtagttg
     4621 acggacattc ataatattga ctaccacaac tgctaaaatc gcaccaaaaa tagctactcc
     4681 ggtcacagga atctctaagt aagaagctat agcaaaacct aagaaaaaat atggtgcaac
     4741 ttgtttgttg atcagcaaac gagcaagcat cgcaaaacca agagctggaa tcaaccctgt
     4801 agctactgac aacccctttt gaacgaagtc aggaatcata tttaatagat ttccgatcgt
     4861 attgcttccc accatataag aaaccattac cacaacacca agcatcaagg aaagaccgat
     4921 accggcaagc aagtgcatat gctcgactcc tttataattt ccttcttctg cataattgtc
     4981 tgctttatgg ttcataattg gaatcaaaag ccctaagtaa atatttttca atatcaatgt
     5041 caacgtagca atcggcaaac ctagtaaaag cgcggtttct gtaccggctc cagctgtaat
     5101 agcaaaagcc gtccctaaaa tcccaccggt cacaacatca ggtggaatcg aagcacctac
     5161 tgagaacgat ccgataaatg ctaactctaa agttgcgccc ataatgatcc cagtttttac
     5221 atcacctaac acgattcctg taaacagccc cgtcacaatc ggacgagaaa gcaacgatgt
     5281 ccctagtgca tattctgatt gtgcgacaaa tgcaacaatt cctaataaga ctgcttgtac
     5341 aatcattgag gttcttcctt tcttacaaaa cttctcttac atccactttt ttatctgctg
     5401 gtacttgacg gatttctact tctatttgtt tgtcaattaa tcgtttcaag cgttcctcct
     5461 cttctggtaa aatatttacc gcttttccga tgctgcgagt accaggtttt gccttgatcc
     5521 ctcctaagtt gatggttttg atttctggat acgcttctgc cagcttatat gcatcctcga
     5581 tcgattccac tacgatgaat aacttatatt tatctgtcac cccgcttttc aacgcggcaa
     5641 tcgagtcatc aattgatttg atcactagtt tgactccttg tggcttggct aatttgatcg
     5701 ttgtctttcg tatctcattc gtaggtacat catcattagc aatcaaaata cagtctgcgc
     5761 ctattgtttg tgtccatgaa aaagctactt gtccgtgtaa taaccgatgg tcgactcttg
     5821 tcaatacaat catttttctt cctcctaaaa ttcgtcttct tctattttct gattgagccg
     5881 ctcgttacaa aactggatca ttttttttga atcagttagc acctgctgga tcaaaacagt
     5941 tgtctctact tcagcgtcca accgagatac tagctcaatc aatagtggca aattcaatcc
     6001 ggcaacgaga tagacatgat cccgattgat atattgtaag aattcgttgt tgacacttcc
     6061 gccaaaaaga tccgtcagaa caatgatttc tttatctttg tacctcataa aaagctgcct
     6121 tactgtttcc gctaaatcaa aattctcatc agtatagcaa tcgaatacag taatagaatg
     6181 atttttccca tagatcagct ctaatgaatc taatatccct gaagcaaatt ttccatggga
     6241 agctaagata atttctctat ccactttttc atctcctctt ttcacttaca catactaaaa
     6301 aagcaagttg catgccaaaa ttagtggcaa caacttgctt atgttcaaaa ttcttcttct
     6361 atcgagagaa actctgtatc ttcactcaaa atatcataga tataggcaat ctcagaattg
     6421 gggatcatga cactataatg ctcttcaatg acactaaacg cagatttaat agcagataac
     6481 ccttcttttt gacactgctt ccatttctct aatccttgat aggtttcgat tggcatttgt
     6541 cggatcaatc gttcaatgag acagctcaca tggacataca acgctaattt tttgggattg
     6601 ctgatgcttc gattcaactg cacctctaat tctctcataa aatgatcgat ttctcccatc
     6661 accttctctg tatctagaat agtcacggaa tccagtactt tttccaatga aaaattacga
     6721 atgatgcgct gattgaatat ttccttctct gaagcattca tagtagtgtc caaccattca
     6781 gctagtacag acatcttttc ccccgagatg aggtcttcta atgaaagata gtcgatattg
     6841 gtgatatcag gatctgtggt gccaatcatt cctacgactt cgtatatgga aaataatggg
     6901 tcactgtttt tcttatcttt taattgctga tattcatatg ggatgatttt caactgacaa
     6961 gccgtaggta agcttttttc tagcagttca cttaagtgga ccgccgtacc gattccagtg
     7021 aagcaagtag taatgagtgc tttgggtttg ttttcctctg gatagagcag tcgccattct
     7081 ggctggatct gtcgaacagt ctcttctgct atctcttcta atgatacatt tttttgcagg
     7141 cattccccta ctgtgattgc taaaggtgtc gtcacattat tcaaaatcaa aagcggtacg
     7201 gataactgtt ttttaaaaaa ctgatggatt tctttcagag atcccatgtc tactaatatg
     7261 atcaatccat ttgaaacatc gctgttttcg caataatcaa ggatttctga agcaatctgc
     7321 tgtggtgtga catctagtgg catatcaaat gattcaaata gatcctcttg taataaacga
     7381 tttactacat ttgccatact gctagcagtt gcataaccgt gagcagcaat catcgctttt
     7441 ggcagtcgtt tttcttttga ccatttcgct ttttttagat agatcgtcag taaaatccga
     7501 tccatacttg tgtcttcaat atctaatttg atcttactta attccagaat tcgttggaca
     7561 tagtgataac tagatggata agactgagaa acttgttgtt ctaattcttt gattagttgg
     7621 ataatatcat gttcttccgg taaccatttg atagtgcttc gctgatagag atagtaactc
     7681 aaggcataga cactgtttcc atcgaatttg atttgatagg cagtttccat ttgtcgaaag
     7741 gtctctcgga tagattgtgt ccaataaacc agcagctcgt gtttttcttg tctactggtc
     7801 tcaaacagca aaaaatcaaa taattgaatc acttcttgct ttaattttcc ttcgcaagaa
     7861 tatagcagac agttattttt cttgaaatca attaggattt gctcaaaact tttaacgatt
     7921 cgctgctgtt cgggctgttg cttctccatt aattgctcta aggtctggtc ttgcgtaata
     7981 tagattcctt gctgtgctgt atttttagta ataggctgcg caagtagttc tttttgcaaa
     8041 tgatggatcg tgacatggat ttcaggctgt tcacgctgtt cagcatatgc ttttgctgct
     8101 gaaactttga cgatattttt cagctctcct atgtttccgg ataatttctg acttgttaaa
     8161 agtgacaaag ctggtccact gactgacaaa gggctcccca tttttctttg ttcttcgaga
     8221 aaaaaagagt aaatcagttc tagccgttca ttacgactac gctgattaag tgcaggtagt
     8281 tcgattcgga taggtattct tcgaatgaaa gttgttaaga aatgactagt aatctcttca
     8341 gtcgtagcaa aaaagagacg tgttttgatc cggcgtgaca aattaggttc tcccatacga
     8401 taaatcacac cttgatcaag ataggtgaac agtttttcct gaccttctgg tgttaagcga
     8461 tgtacctcgt ctaaaaacaa gatcccacca tctgctgctt caaaagcccc ttttttattt
     8521 tcttctgcac cagtgtacgc tcccttaaca tgtccaaaca aattgctagt caaaagttca
     8581 ggattatctg catattgtgc acaattaaaa gtgatgaatg gtgcagaatc ttctagcaga
     8641 tcatgaagca gacaatactg atagaccaaa ttaactaaat aacttttacc tgtgccactt
     8701 tcgcctgtaa tcaaaagtgg taatccgcca tctggatagt aaagagctgt tttgatttgt
     8761 tcgatacttt ctttcaaact ggcgtcgtat ccgatcataa aagagaataa gtcttttggt
     8821 tgcgcgaaaa aaggctgttc ctctttgaga tcatcgatag aaaaataaaa attcgtgcgt
     8881 aacttaaaaa attgtttttc aaaagcttct ttgtggaaat aaatcactgg tcgagaattg
     8941 attttgacta gtagttgctc atcatttaac tggttcaaat aatgactgac tgtgtttctt
     9001 tttaccccaa aatgatcagc taacctttgt gctgtaaaga cctcgttcat atgttctaaa
     9061 tcgaaaaagg ctgtctggtt ctctaagtaa cataaaattt ccttcttcaa caacttattc
     9121 acccctcctc catcatagaa aacggattca ttttgtcaat aaaatcttta ataaagaatt
     9181 gaaaaatata tcttattata gacagcagcc tacttatccc ttataatttt tattctaaaa
     9241 aacgtacagt tccttccagt ttttatactg gaatttcaac atatgaaaga ataaggaggc
     9301 tatgactatt caatgaaatc ggataaatat aaaaacagga aacatacttc ctggaaaact
     9361 tttcgagaaa tcatcggatc ttatctcgct gtagttgtat ttttgccttt gctttatatg
     9421 gctatgacag aacaaactcg ttttattggc gtagatacgc aatatatagc cgaaacagga
     9481 tttttattca tctttatctt gatttttgcg ttctataaat ccttccatac aaaataagat
     9541 tcacagattt caactttcct gtctgttttt agctattaca aatccttata tgtggtataa
     9601 caacaattta ttggcataat gaggaaaaac gagttatgat gctttcagag agtaaagagg
     9661 tactgttgtc accatccttt ttattgatta cgtacaccac tcttagtttt ttcccattga
     9721 ttacgaaaac aaggtagaaa cattgatgtt cctaccttgt tttctttttt tattttcaga
     9781 tcaaaatctc aagtagcttt tgacttgatg tcgggaggtt gtcttcgttc catcaaacct
     9841 gatagaatct gcgccaaacc atagttttct ccactattat ttattcctct aagatagtac
     9901 ccacaataaa cgaataaata tttttctcat tttcatactc cttctttgaa aatcaattag
     9961 atttctctca acccactttt ttacaaaaat tacgtagcaa aaaagattgc aagaacaaat
    10021 taaaaaagaa gtaaatagag tttcatctat aaacatttac ttgatatatt ttttatgcag
    10081 aattcattta tagaaaaatt aagtaaaata gacgttccta tgaatgaaga gactggcatg
    10141 ttttctcatt ttcttttttt taaaaaaaat agtctattca atttctaaag cttcttcata
    10201 ataaaacttt catttatgaa aaggcttgtt ctcttttcat tctaaatttc ctaagctaca
    10261 atgtggtagt acttgttttt tcaattgtgg acaaataaat aataggagga atcatatgat
    10321 tcagttatta cgttatgcaa aagactatcg taagcaaatc attttgggcc cgttcttcaa
    10381 atttcttgaa gctgtcttcg aacttgtcct gcctttaatg atggcttcct tgatcgataa
    10441 tggactaaaa atgaatgacc gaggaaaaat cattgaaatg ggcttatgga tggtcgctat
    10501 gtctgtgatt ggtctgattt gtgctatcat ttgccaatac tatgcttcag tagcttcaca
    10561 aggatttggt acagagttac gtaatcagct aatcaaaaaa atcaatactt tctctcacaa
    10621 cgagttgaat cattttggaa cagatacctt gatcacccga atgacaaacg acatcaatca
    10681 gcttcagcta gctttagcaa tggtcattcg tttattgatc agagcaccgt ttttaagtat
    10741 cgggtcagtg gtcatggctt ttgtgatcga ctgggaagta ggtctgtttt tcttagcatt
    10801 attgcctatt ttctcaatca ttttattttt tatcatcaaa aaaaccgttc ctctctacca
    10861 aaaagtgcaa gaaaaactag atcagctcaa tgaacacgtt agtcagaatc ttagcggtgt
    10921 tcgtgtcatc cgcgcatttg cgcagacaaa aaaagaaaca gagaagttcg ataaaagcac
    10981 aaatgatcta gcgaacaatt atttacgtgt ttccaatatc tctgccctat tatcacctgc
    11041 tacaaccttg atcatgaatg tcgggatcat ctgtttgctg acagttggcg ggatcaaagt
    11101 aaatattggt agcttgcaac aaggacaagt tcttgctttg atcaattata tgaatcaaat
    11161 gcttttggca ctgatcgttg tttcaaacct cgtcgtgatc ttcacacgtg ccgaagcttc
    11221 aggaaatcgg gtgaaagagg tgctagacac agaaaattcg attcttgaag ctgaggtcag
    11281 tactactccc gatttttctt ctgaagcaat tatcgctttt gaccatgttg acttccgtta
    11341 tactcctgat tctggtcttt ctctccaaaa tattacattt cagctgaagc gaaatacagt
    11401 ccttggaatc attggtccaa ccggaagcgg gaaaacgact ttgacacaat tgattccgcg
    11461 tttctatgat gtcagttccg gtacagttat aatggacgga atggatgttc gttcttggca
    11521 gctggatcat ctccgtcagc aaattgctct tgttccccaa acttctgtac tatttaccgg
    11581 gactattcgt gaaaaccttc aatggggtaa gaaagacgcc acagaaaaag aatgttggga
    11641 agctctgaaa atcgctcagg cagaagaatt tgtccgtcag cttccaaatg gtttagatac
    11701 aaaagtcatg gaaaatggga aaaatttctc tggcggacaa aaacagcgac tgacgattgc
    11761 aagagctttg attgccaagc cagcattact gattttagat gactctttaa gtgcgttgga
    11821 ttatcaaaca gacttggatt tgcgtaaggc gctacagact tatcttggca gtacagtgat
    11881 tattatctcc caacgtgtcc gctctattca agaagcacat catatcttag tgatggatca
    11941 aggtaagatt gctgctcagg gtacccatga agaattactt gctcaatcag cagaatatca
    12001 ggaaatcgtc gcatcacagg aggaagaata gttatgaaag taaaaaaatt atccttgcag
    12061 acgctccgcc gattcattcc ttaccttact gcctatgcaa aagacatttg gctagctgtg
    12121 atactaggaa tcatcaatgg gattgcaaca gttgtgatga cgttacaaat tggtcaaagt
    12181 atcgatcaaa tgattggaga aggacaggtc cgcttcaatc gactgttcca tttacttatt
    12241 ctattcggtg gtatcgtttt aatcaacgta gtcagtcaat ggctcatcca agtgttaagc
    12301 aatcggttag cttacttatc cgttgcaaaa ttaagaaaag atgcatttgc tcatctgaat
    12361 cgattgccac ttcgttatta tgatcaaaac tcccacggaa atatcgtcag tcgatttaca
    12421 aatgatatgg acaatatttc cattgcaatt tctgcggcct tcaatcaatt attttctgga
    12481 gtggcaatca tcattctttc gtttattttt atgatgcgtc taagtccact tctgacattg
    12541 gtcgttcttt gttctacacc aattattttc cttgtgagtt ggatcgttgc tcgtgcttca
    12601 caaacaaatt tcgatagaca gcaaaggatt attggggata tctcgggctt tgtgaccgaa
    12661 cgaatcggta accagaaaat cgtgaaagct tttcagcaag aagaagctag tcaacagcaa
    12721 tttgaactgc taaatgctga tctatatgaa aaaggacaac gggcacaatt ttcttcttcc
    12781 ttgacaaacc cgtcttctcg ttttgtagat caccttgctt atttatcgat tggtttagtt
    12841 ggcggcctat tggcattgaa gagtggttcg tcaatcactg ttggtgtgat ttccagcttt
    12901 acgatctact caagtcagtt ttctaaacca tttatcgaac tttctggaat cacgacacaa
    12961 ctgcaaaccg ctttttctgg tctagaacga gcatttgatt tgatcgatca gccagaagaa
    13021 aagcctgatt tgccagatgc ctatgccttg gaacccaaaa atatcaaagg aaaagtctct
    13081 tttgatcatg tttctttttc ttatgacccc aagcagcgtt tgatcgaaga tttcaatttt
    13141 actgcttatc caggtcaaac cattgctata gtaggaaaaa caggggccgg aaaatccaca
    13201 cttgttaatt tgctgatgcg tttttatgac acaacaaaag gaacaattac agtcgatgac
    13261 catgacatcc gctctatcca acgtgataca ttacgtaaaa gttttggaat ggtgttacaa
    13321 gatacttggc tatttgacag tacattgcga gaaaatcttc gttttgggaa tccggaagca
    13381 agtgacgcag aaatcaatga tgccctaaaa gcctcttata tgtatgaatt tgtagaacgt
    13441 ttgccggaaa aattagatac taagattggc ggtcaagggt taaagatatc agatggacaa
    13501 cgccaactgt tgacgattgc tcgaacgatg atcagtaatc cccctatgtt gatcttagat
    13561 gaagctacca gctcagtcga tacattgaca gagaaaaaaa tccaacaagc cttcttagca
    13621 atgatggaag gaaaaaccag ttttgtgatt gcccatcgcc tgtccacaat caaaaatgcg
    13681 gaccagatat tagtgatgga tcagggagct attgtcgaaa tgggcaccca tgatgagctg
    13741 cttcaaaaag atggttatta ccgacggctc tatcaagcac aattcacagc acaataaaaa
    13801 caagcagtct tctgggatgg gaagactgct tgtttt
//
LOCUS       DAFBQH010000076        13533 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.078, whole genome shotgun sequence.
ACCESSION   DAFBQH010000076 DAFBQH010000000
VERSION     DAFBQH010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.078"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(57..1004)
                     /locus_tag="LK694_002273"
     CDS             complement(57..1004)
                     /locus_tag="LK694_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBK5053108.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1194..1463
                     /gene="rpsN"
                     /locus_tag="LK694_002274"
     CDS             1194..1463
                     /gene="rpsN"
                     /locus_tag="LK694_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBK5053109.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1639..2001)
                     /locus_tag="LK694_002275"
     CDS             complement(1639..2001)
                     /locus_tag="LK694_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBK5053110.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2012..3133)
                     /locus_tag="LK694_002276"
     CDS             complement(2012..3133)
                     /locus_tag="LK694_002276"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBK5053111.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYIHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3149..3499)
                     /locus_tag="LK694_002277"
     CDS             complement(3149..3499)
                     /locus_tag="LK694_002277"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBK5053112.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGKVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3629..5140)
                     /locus_tag="LK694_002278"
     CDS             complement(3629..5140)
                     /locus_tag="LK694_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBK5053113.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQSETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5462..6814)
                     /locus_tag="LK694_002279"
     CDS             complement(5462..6814)
                     /locus_tag="LK694_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBK5053114.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKDFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVPDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAVKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(6957..8042)
                     /locus_tag="LK694_002280"
     CDS             complement(6957..8042)
                     /locus_tag="LK694_002280"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBK5053115.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFLKKMNKEK"
     gene            8238..9983
                     /locus_tag="LK694_002281"
     CDS             8238..9983
                     /locus_tag="LK694_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBK5053116.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDSVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLKKEEQTQASRKTEIVHEKEFGSFAFRASTSTEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10232..10699)
                     /locus_tag="LK694_002282"
     CDS             complement(10232..10699)
                     /locus_tag="LK694_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBK5053117.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFCQALKKAIDQGLPSTEDSR"
     gene            complement(10683..12056)
                     /locus_tag="LK694_002283"
     CDS             complement(10683..12056)
                     /locus_tag="LK694_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053118.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGIMYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDAAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12377..12865)
                     /locus_tag="LK694_002284"
     CDS             complement(12377..12865)
                     /locus_tag="LK694_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5053119.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(12887..13489)
                     /locus_tag="LK694_002285"
     CDS             complement(12887..13489)
                     /locus_tag="LK694_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBK5053120.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3862 a         2938 c         2458 g         4275 t
ORIGIN      
        1 tgtctttgcc cctacaaaaa tgtttcagct tttttcgttt tttattcctg tatttcttat
       61 ttttcgaatg acaggatgat tttcccagca ggaggatgtc cttcaactag ctgatgtgct
      121 tggatgacat gcttaaggga agcagacaga ttcattgaaa taaaaacatg aaaggctcct
      181 ttttggtagg ctttgattat gccagcaaag gcttctgcat ctagatattc ctttcttgga
      241 acaaacgatt cataaaagcc ttcctttttt tgccgcaaat ctagatctgg gaggtcattt
      301 aatgcaacat acctgccgcc tggtttcatg atctggatgc cggtttctcc tttgatactt
      361 cctttcgtcg catcgattac aaggtctgcc tgatcagcaa attgcagacc tggatttgtt
      421 ttatcataag cagcaaatgc tgaagctcct aattttttta ccttttcctc atttttactg
      481 gatgcactgg ttaagattcg gattcccttt tcatggagaa gctggatcag acttgacccg
      541 accgcacccg atgctcctag tatcattaca gtatctgtcg gctggatttc cgtcaaatgg
      601 ttgattagat tataagccgt aattcctggc gtgaccattc cggctgcttc ttcccaactc
      661 attttatctg gaatttttgc tacttttgct gatggcaaca cgacttcttc tccgtaagtc
      721 ccgccgactg catggacagc tactcgatca cctacatgaa cagttgtgac atctgaacca
      781 atctctgtca caataccagc gccatcatta cctggtacat aagggaattt caatgttcgt
      841 atctctttca tttctcctaa acgcaaggca acatcataag gattcacact gaatgctttt
      901 atttctacac gaacatgtcc agaaccgact tctctgggat gagcatcgat ttcttcaaaa
      961 acatcttgtg cttctccgta accgttaatt gcaaatcttc gcatggtcac catctccctt
     1021 tttctttaag tttacgttta tgcctttatt ttgacaaaaa aaatgtcttt tcgttttggc
     1081 gcatgattca aaacaaaata gttgtctttt ttcgcgtaac gtgttaaatt atttatctaa
     1141 aagcgtaaag attacgattt acgctaataa aaaataattg gaggaatttg ttgatggcta
     1201 aaaaatcaaa gattgccaaa gcaaaaaaac agcaagaaac gattgaacgc tatgctgctt
     1261 tacgccagga attaaaagcg caaaaagatt acgaaggctt gcgcaaactt cctttagatg
     1321 ctcgtccaga acgattgaaa aatcgtgatt tgatcgatgg acgccctcgc ggatacatgc
     1381 gtaaattcgg gatgtcccgt ataaaattcc gtgaattagc acataaaggt ttgattccag
     1441 gagtaaaaaa agccagctgg taatccaagt ctggctgtaa tgtaaaggag gagggcgcat
     1501 ggaccactcc ttggatcatc aaaaaagata cgttcgcttc ttctagcaga aatgctggta
     1561 ctagaagcag acgtatcttt ttttacgcac agctggtcct tttttatatg taaaggctgc
     1621 atggttactt cgctatattt atttagtaac aggagtcagt gcttcgatac ccacactaat
     1681 tttcaatgct cggtcaactt ctgccataat gggtcgatcc aaatggcaga ccttttcttt
     1741 caatcttgat ttatcgatcg ttctgatctg ctccaacagg ataacggagt ctctttctat
     1801 tcctgttcca gtagctttga tcccaatatg agtcggaagc ttaggtttag ccatttttgc
     1861 cgttatggcc gcaacaatga tcgtcgggct aaaatggtta cctagattat tttgaataac
     1921 caaaactggg cgtgttccgc cttgctccga tcccacgact ggagaaagat cagcaaaata
     1981 aatatctcct ctttttacca taggatcctc cttattcttg atattctctt ggtatccgct
     2041 gtcctaatcc gcaagccact tcatagtgga tcgtacctaa ttgatctgct atatcctgca
     2101 tagtgatttc tttgtctgca tttttgccga taagcgtcac ttttgttccg acaggaaatt
     2161 cttgaggcaa tcggatcatc agttgatcca tacatacacg cccaatcgtt tcacagtagt
     2221 tcccttctac caatagagaa aaaccctgca tctttcgtgg ccagccatct gcataaccga
     2281 tcggtatcgt tccgatccac tctgcttcag gagtgatata tgtttctcca tagccgattc
     2341 cttcacctgc tgggagtttt ttgacttgaa ttaattcaga aaccagctca agtgctggct
     2401 gcagtggata aacttccggt agtgcgtgcc cagagggatt cagtccgtac attgctactc
     2461 cataacgaat catattgccg attgtttcat catgccataa agccgttgca ctattgctga
     2521 catgtatata tcgaggaagt tcgggaagtt tttttagcac ttctataaag cgttcttttt
     2581 gcagattcca atatgtatca tctgcctgat ctgctgtaga aaaatgggta aataaacctt
     2641 cccattctaa tacacgactt tctttgatga atgcagctgc ctcttttact tcttcaggtg
     2701 tacaaaatcc gatacgaccc atcccagtgt ctgcttttag atgaatcgaa agaggcgtct
     2761 ctagcttact tttctctaaa acagcagctg ctgcctgcaa ccattcccgt gtacctgcag
     2821 ttactgataa atcatgagca acagctacag tcagtgcgtc gtaaggaacc atattcaaga
     2881 taaggatggg ctgagtaaat ccagcttctc tcaactcgac tccttcatct aaatttgaaa
     2941 cacaaaagcc cgaagctcca cctgcaactg ctgcctcagc tgtttcaata gctccatgac
     3001 cgtatccgtt tgcttttact actgcaaaca attcttttcc ttgcggaagt cttgcaactt
     3061 catttgctac attttcagtt atagcttttt tatgtatcac tgctttcgtt gggcggtgcc
     3121 aagcgacaac catcgttttt ccttctttct agtcttccaa aataatttgt gcaaaagctg
     3181 tttcatttgt atgagtaata ctgacccata ctttgccttc atgaggtgaa cgcgtcacaa
     3241 caggctgacc gttttcattt tttaaaatct cgacatcttg aaaggtaact tttccgatcc
     3301 cagttcccca agctttagag aaagcctctt tgcaagcgta acgcccgcct aaaaattcta
     3361 cttggcgatg aaaaggcaag gactggaata gtttcatttc atcagaggtc aagattcgtg
     3421 caatgaattt tggtttttct tcgatcagcc gagtgatcct aggaagttcg actgcatcga
     3481 tcccaattcc ttttatcata tgcttttctc ttttcaataa atttagaaca ctctcattct
     3541 accaaattat aaccattttg ccacaaataa gtccacaaat aacaaaaatg aaagtttctc
     3601 ttaaaaaaga gaaactttca ttttatgttt aatcgttatt tgaacggatt acaaatccgc
     3661 gttttttatc gttgtgacgt ttaccgccat ctttttggct gttattattt ttgctataac
     3721 cgccttttga tttgtttcca cgataattac cgccgtcacg acgattacgg ttgtttccgc
     3781 caccacgttt gccattttta ttgtatcctt ttttctgcat tggcaatggg cgttctggtg
     3841 tgatcttcac tggcactgca tcagctggat cttttgctgt cgtttttagc aaaagtgcaa
     3901 ctaaatcttg tgcagaatat tcttctaata atttatctgc tgtttgcagg tacttatcta
     3961 gaccattttc tgcaagtttt gtttcgattt gttcaactgc agcacctaat tgacctttga
     4021 atgcttcttt ttcggttggt ggacgtaaag tcgtcatccg ttttttcgtc aagttttcaa
     4081 tcacatgcaa gtagtccatt tcatttggtg taacgaaagt cacagacata ccgccttttc
     4141 cggcacggcc tgtacgtccg atacggtgaa cgtagctttc tggatcttga gggatatcat
     4201 agttgtacac atgagtgaca cctgaaatat ctaatccacg tgcagcaacg tctgtcgcta
     4261 caaggatatc taaatgaccg ctcttaaatg aacgcaatac gctcattcgt ttttgttgag
     4321 acaaatcgcc atggattcct tctgcacggt aaccgcgtgc ttctaatccg cgtgccaatt
     4381 cgtctacacg gcgttttgta cgtccgaaga cgattgttag ttcaggtgtt tgaacatcaa
     4441 acaagcgagt catgatatcg aatttttcgt attcttttgc gcggacatag tattgatcga
     4501 tcaagtcagc tgtcatttct ttcgctttga tcttcacatg atgtggattt ttcatgaatt
     4561 taacgccaat attcttgata gctggcggca tcgttgctga gaaaagtaat gtttgtcttt
     4621 gatcaggaac ttgtgaaatg attttttcga tatcttccaa gaagcccata ttcaacattt
     4681 catctgcttc atccaaaact aatgtttgaa cggttccaag tttcaatgtg tggcggttga
     4741 tgtggtcaag catacgtcct ggtgtaccca caacgatatg tggacgatct ttcaagccac
     4801 ggatctgacg gccaatatct gctccgccgt aaacggcttg tacacgtact tttttgtcgc
     4861 gccctaaacg atataattct tcttgtgttt ggatagctaa ctcacgtgtt ggtgcgataa
     4921 ctaaaccttg taattcgtga cgatctggat cgattttttc taacattggt aatccaaatg
     4981 cagctgtttt ccctgtacct gtctgtgctt gtccaataac gtcttttcca gctaatgcta
     5041 gtggaatcgt ttctgattgg ataggtgttg cttcttcaaa accagcgcgt tcgactgatt
     5101 tcaacaattc tggtgataat tctagttctt taaatttcaa atgaatcctc ctatatggtt
     5161 gttaactgag gatgtagctt ttttgctacc ccgtcgattt gttcggtcaa agtgtattgt
     5221 ttctttttga cagtggccac gcacgttttt atccattctt agcccctgtg gcaccaccga
     5281 aaaatcactt tgcgaagttt aggcattaga aaagccaaac gggtcgcgtt cagctttatc
     5341 attattatga catctggctt caacaagttg tttctatggc aaaaccagtc caatttctct
     5401 tcatctttcg gcaataggtt attctaacat agtttttcgt tttctgcaag tttacagatt
     5461 tttatttcat ttgctgaagc tgttctacca cttcaaataa gcccatgctg ttgctgcctt
     5521 ttagcataat actatctgtt gcttcaagat tttgttttac agcatcaatc aatttattct
     5581 tttctttttc aaaatagaac acaggtagtt gggggtattt ttggttcaat tcttctttta
     5641 gtgcatgcat ttgttcccca taaaggaaaa tcatggcgaa tttctcagga tcgatatgtt
     5701 cactcatttg cgcatgcata tttaaagact ctttccctaa ttccagcata tctgctaaca
     5761 cagcgattct gcgtcctttt gttggtagat ttttaaaagt atctaatacc agtcccatgg
     5821 ctgttggatt tgcattatag acatcgctca aaatagctgc accatttcct gccgttagcc
     5881 attcggttct gttttgagtg atagaaacat gggcgagccc ttggcgtatc tcaggtatgg
     5941 acagtccaaa gaagcgcccg atcgtatacg caatcaaagc attcgttaca ttataacttc
     6001 caggcagcgg gatcgtaaat gtttctcctt ctactacaaa atcggtttgt gcttttcctt
     6061 ttgctgtcac ttccgcatga acatcgccat ttccaatacc gaatgttgtg actgtttgta
     6121 ctaatgttgc cgttaaaggt gtcagcaacg gttcatctgc gggcacaacg agtaatccgt
     6181 ctggtacaag tccgtcagtg atttccattt tggctttagc gattccttct cgcgacccaa
     6241 gattttctat gtgtgcttca ccgatcattg tgattgctgc agcttttggt tgagccataa
     6301 gtgaaagttc cgtgatttca ttggcgtgat ccatgcccat ttccaagacg atcttttctg
     6361 tatcttgggg catgtgcaaa atcgtgtaag gaagaccaat attattgttg taatttcctt
     6421 gcgtcttata tgttttgtat ttttcagaca agacagacgc ggttagatct ttcgtggtgg
     6481 ttttaccatt gcttcctgta attgcaacga cttctgcacc tactttttcc aagtaatagg
     6541 ttgccaattg ctgcatggct ttcaaaggat ctgctacagg aataactgga aaatcttttg
     6601 gagcttgttc aacaggcaag ctccaaaatg aagcagatgc tccgtttgtg atcgcttgct
     6661 ggataaattc atgtccatct cgcgtaccag caagaggaac gaataaactt ccttcggtta
     6721 tcttccggct gtcaaattcg acattggtaa tctttgtcag tgcagagcat tcgctgccca
     6781 cggcttgtgc aatttcttgt attgttagat tcattgagat ctctcctaaa aaaggttgtg
     6841 aaaaaagcgt ttggcctaaa cggcgggttc gcatgactct cacttccaag taaatttctc
     6901 tattcgtatt tagaagctag aatgaacgcg gttacgcaat cactccagct tcttctttat
     6961 ttctctttat tcattttttt caaaaaagat tgacgctgat ggtatcgatt cattcctaat
     7021 tgaatcagtt cttcaatcaa atcaccgtat ttcaagccca tattttccca taagagtggg
     7081 tacatactga actccgtaaa tcctggcata gagtttaatt cattcaggaa taattcgttt
     7141 ttatttgtca aaaagaaatc gcaccggctc aatccgcttc cacctaacat cgtgtaagct
     7201 aacttcgcgt actcttgcgc tttttgataa acttcttccg gcacttcggc tggaatctgc
     7261 atttcgattt tattattgat atatttggct tcataatcat agaatgctac gtcttttacg
     7321 acttcgccag gcaaagtcgt ccgaacatct tcatttccta atacagcaac ttcgatttcg
     7381 cgcgcttcaa ttccttgttc aacgattgct cgagaatcat actgataggc tgttgctaaa
     7441 gcattttgca gctcttctcg gttttctgcc tttgtaatgc cgacactaga acccatattc
     7501 gcaggtttga caaacatcgg ataaagcaaa gaaccttcac attgatcaaa tactttttta
     7561 ggattttctt tccattgatt cttaagtact ggtacataag gaacttgcgg cacaccagca
     7621 gcttgtaaaa tatacttggt catgatttta tccatggcac atgcactggt caatacgcct
     7681 gcgccgacat aaggcatatt caatgtctct aagaagcctt ggatcgttcc atcttccccg
     7741 tttggcccgt gtaaaactgg aaaaacgatg gctccttctt ctttgatttc tcccggattg
     7801 atcacttttc ctgtaaagcc ttcctctgtc tgtccacttg ggtcccatga aagatgcaag
     7861 acatctttgc tagcaggttt ttctgttagt aatggacctt tgacccattg tccttctttt
     7921 gtaataaata cgagttgaac ttggtaataa ttataataaa tggcatttaa aactgaaaat
     7981 gcggaaagaa tggaaacttc atgctctgcg ctgcgtccgc catatagtaa agtaatcttc
     8041 aaaagattgt cctcctgtat tctaaatcgt tcagtgattt actctcatat tttagcacaa
     8101 aacaaaaata aacgaataac ttatttataa aaaaacgagt tggtttctaa aagaacaaac
     8161 gtcatttatt ttttgtcagc tagattattt gtcccgtttt ttataaaaag ttatgataaa
     8221 gaaaaggagg gatgatgatg gcgcaaacaa atccacgtag ccgcttggca gaaatcattc
     8281 gagtgttcat caattacaat gtagtgccaa actttgtaca gcaaaaaaat cccgaacagg
     8341 taaaaaaagc ctttgaagaa ctaggcccca ccttcattaa gataggacag atgctttctg
     8401 ttcgagaaga tttgctttct tctgccttca ctcaaacatt caaaacatta caagatagtg
     8461 tacctagcga cacattttca acggtcaaaa agacgatcga aacagaactt tctctttcat
     8521 tatcagatat attcgatgat ttttccaaat cgccttttgc ctctgcttct atggggcagg
     8581 ctcatcgcgc caagctgaag aacggtgact cggtcgtcgt taagatccaa caccctaaca
     8641 tcgccgaaga gatccgactt gatcttcaac tatttgaacg agcgatccct ttgatcaaat
     8701 acattcctga aactagcgta gttgatctaa aaggggtact ccaagaagta aaacgttcct
     8761 tgatcaatga aatggacttc ttaaaagaaa gtcaaaatgg cgaacaattc tatcaaaaga
     8821 acaatggctg gaaagagatc cgcagcccca agatttatga cgctttttgt tcgaaaaaag
     8881 tgatcgtgat ggaagaaatg tctggtaaaa acttgaacca tttaatgaac gccgaaaaca
     8941 aaactgaaac ttttatcact ggaattcaaa acaagcagct gaaacaagaa gttgcaaagc
     9001 ttttagttga gaactttatg aaacaggtat ttgatgatgg ttttttccat gctgatcctc
     9061 atccagggaa tctgctattc catgtactaa aaaaagaaga acagacacaa gctagccgaa
     9121 aaactgaaat agtccatgaa aaagagttcg gttcctttgc ttttcgagcc tccacttcta
     9181 cagaagaccc tgttgcgcca tataccatca actacatcga ttttggtatg atggggcatc
     9241 tttctgcagg acttaggcaa aaactcacac aagccgtttt agcgctgtat acgaaagatg
     9301 cttatcgaat cgaaaaagcg gtccttcgac tttgtcagca agaaggctcg tttgatgaaa
     9361 gtcggttcca ccaagaactg acaagttttc ttgaacagta ttatgattct cctattgatg
     9421 aaatcaactt gcaagaagta ttcacacacg tcgtgacgat ttgtcatcaa aataatctgc
     9481 agttcgatcg tgatatcacg cttttgctga aagcatttgg gacattagaa ggggtgatcc
     9541 gcgtccttga ccctgaagtt tccctcatgg aagtcgcttc gccttttgcc caacattatt
     9601 ttctcactca tctggatgta gaagatacat tgaaacaaag cggattagac ttattggaag
     9661 ggatgaaggc tgcgcctaag atccctcaac agctccacca tctattagaa atgtggactt
     9721 cgggacaagg aaaagtcaat ttggaattga aaaagcaaga caaattattg agcaggatcg
     9781 aaagtatgat caatcggctt gttttcggta tgattttagc tgcattgatc gtagggtcct
     9841 ccctgcttgt ccaagcagca cctgtagaaa acgcagaggt cgtttcctta ttagggatat
     9901 ttacgtatgc tattgccgca ttcgtcatta tttttttggc aatcgatgca ttgatccaaa
     9961 tgtataagaa gaggaaaaag tagaaaaagg ttgtgacaga aagaatcagc ttcaagaaat
    10021 aagaagtgat tttgcataaa ttgtttctca aatttttgtg aaaattcggc ttatttttcg
    10081 aagaggctac ttctgaagca ccgagaataa aaaatgtgaa aaagctgttc tgcacccagt
    10141 aataagaaca atcaaaaagg aacatggagc acatttatcc tgtttccctg ttcccttttt
    10201 gatgtattcg agcataaata aaataccttc gttacctact atcttctgta cttggtaaac
    10261 cttggtcaat tgcctttttt aacgcttgac aaaaagacat atgatctttc tccgtaaagg
    10321 gttttggacc ctttgtcctt atgccagctt ttcttaactt tccactatga taacgttgtt
    10381 ctagaagcgt gccgatattc gcttcgtcaa attcttttcc agagtgatgg aacactcgga
    10441 cgcccttcgg caatacaagt gaagctaaac cataatcttg cgtgatcaaa aagtcattct
    10501 tcttaaccat accgacgatt ttataatccg cactatcagc tccgcggtct acgtagacga
    10561 tttggacatg attagcgtat tctttggtcg tataatggtc cacactggtc acgataacca
    10621 cgtcaagacc ataggattct cctaatttta tgacatcttc ttttactgga gaaccatctc
    10681 catcaatgaa tagcctcatt ctttttcgcc ttcttccata aatgataaac agttactaat
    10741 ccgctcagta aaagaccaac attgatccat tttgccaaga taagcagttg tacactttct
    10801 atcggcactg attcatggtt cagcgtgatc aataatagat ggattggctc tcccatttct
    10861 tttgctaaaa caaacaaaag cgtcatcaat aaaaactggt ggatcacact ccctttattt
    10921 tgagtagtat acttgacaac gatccggcga tccattggaa tagctgcatc agtttgataa
    10981 tattccttat ttagccagct gcctgttact acgtgccaga aactaagcag aaagacacct
    11041 atcgcaaaac catttggaat caagaggacc aataaagaag aactcagtcc aagcaattgc
    11101 atagccaatg gagaccatgt taaacgtttc aaaatccatg gtccgactat ttttgctgca
    11161 atcaagcctg ctgcataagg caaatacata aaaatcgaaa gtttttctaa tccgtacatt
    11221 atgccaacat aaaaactgct aaataaaaaa aggaaattcc ctagcattcc atttagaaaa
    11281 gtaaacagat ttagccagct tggcaacttc cattccttct tctggtgtcc taaaacaaac
    11341 acgaacaaga tgaacagcag caagaaacca taaatagcaa gatcaaaaag cctttcatcg
    11401 aataacaatc gggcatttct caaaagaaac aataagccaa agaatatcaa aaataaaaca
    11461 agctctcgcg tagctaccag atttcttttg atgtctttga aatccagttc gtaacgcgga
    11521 taatgactaa ccgtatggta ggccatgacg taaaaaagtg tataaatagt aaagcttgct
    11581 gcaatctgag tcggtgcagg caactcaatc gcttgataca ggagaaatag ccagatagct
    11641 gcaaataagt actttttccc atccatattg ataaaacctt gcgtcttttc gtgaaaattg
    11701 accgtggtat tagcaggtac aagccatgct gcactcagcc ctagcaaaac acttgaaaaa
    11761 taaaataaag gaaaataaaa tacgcctaat attccaagca atgaaccagc accaccaatc
    11821 agcagagcga tcatcagcaa cgtgtaacta tctagaccaa atcgtagact gttgactaaa
    11881 aatagtccag tcatccgaaa ggcataaaac agcgtaaatg gcaaaatcgt cagccaatta
    11941 tgcgagtctg ttagtcccaa aaataaaaga taaggaataa aaataaccgt gttacttaag
    12001 ataaagggcg ttgtttgtgt aaaattacgc ccaaatcgtt tgagtttttc gaacatgaca
    12061 atggctcctt aaacagtctt ataactaatc tgctcttcga ttttagcatt aaaggggaaa
    12121 taaaccaatt ttacagaata tattcttgta aaaaaaatac agaaagaagc ataagcttct
    12181 ttctgcattt ttacggtact tgattactca agcagacaag cccttctcca caaaagtctt
    12241 gcccgacacc cctgcatccg gcaggtgata aagatagaaa tcaattatcc tgtgcctcca
    12301 aaatcaatta ggagctctgt aaaccagagt ccttctcgac ccgtcgcata aatttacttt
    12361 aacacagttc ctatgtttat gcgagagctt ttttcatttt ataatcgacc atttgaaatc
    12421 cttgaccgat atctgaaata agttcttctg taatctcaaa tccttcatgc ttataaacct
    12481 cgattgcttg atgattcccc tgattcactc gcaaaaagat ctctctttcc tttgtttgcg
    12541 cagcaagttc ttcataccag cgaaaaactg aagaagtcaa tccttgacca cgtactttat
    12601 ttagcaaata taatttactt aaataaattt ttccatttat ctcttcataa gcagtgtaac
    12661 cgacagtctt ttcttcgctt tttaataaaa agtaggacac accttctgtc agttcttttt
    12721 gaatctgttt ttttgactga taagttttca gcatgtaatc cacttgtttt tgaccaatga
    12781 tcggcacaaa agcttccggc catacttctt gtatgactgc catcagctca tccagttcat
    12841 tttctgttac ttttattttt tccatacacc cacgtccttt tttacattat aaatgacctt
    12901 gcgcttgatg gaataaaaag agcatgatca tgctatacac acccaccgac cacggtttca
    12961 agaaaatcac aatctgcata aattccctaa aagataattt cgtcaaacca gctactagac
    13021 agatcaagtc gtctggcgca aagggaagca gcaaggtaac aactagtact tttttaatat
    13081 ctttcgtttt tcttgtcagc aattcatcaa atttctgata cttttttggc gataaaataa
    13141 gctggacaaa ggctacgcca tagtaacgga caagcaaaaa tccgattgct tccccaatca
    13201 ctaggccaat acaactatat aaaagcccaa atccattgcc aaacatcagc atgcccgcta
    13261 ctgaggagat ccctcccggc aaaataggaa tgatcacttg gatcacttgc aacagaacaa
    13321 atatcatcac tgcatacttg ccaaattgct ggataaacgc ttgcaaagac gtgctcgatc
    13381 gaaaaatccc atgatgataa gcataaagaa tcaacacaaa gaaaaaaaac agaccaataa
    13441 tgggcatgat ctgaagacat ttttgtaaaa ctgatccttt tttagacatc tatattcccc
    13501 acatcttttt tcatttatta aaactgcctc ctt
//
LOCUS       DAFBQH010000077        13334 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.079, whole genome shotgun sequence.
ACCESSION   DAFBQH010000077 DAFBQH010000000
VERSION     DAFBQH010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.079"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(180..737)
                     /gene="frr"
                     /locus_tag="LK694_002286"
     CDS             complement(180..737)
                     /gene="frr"
                     /locus_tag="LK694_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBK5053121.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(740..1462)
                     /locus_tag="LK694_002287"
     CDS             complement(740..1462)
                     /locus_tag="LK694_002287"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBK5053122.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1598..2479)
                     /locus_tag="LK694_002288"
     CDS             complement(1598..2479)
                     /locus_tag="LK694_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBK5053123.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="LK694_002289"
     CDS             complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="LK694_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBK5053124.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3719..4198
                     /locus_tag="LK694_002290"
     CDS             3719..4198
                     /locus_tag="LK694_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBK5053125.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4415..5506
                     /gene="phnW"
                     /locus_tag="LK694_002291"
     CDS             4415..5506
                     /gene="phnW"
                     /locus_tag="LK694_002291"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBK5053126.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5499..5627
                     /locus_tag="LK694_002292"
     CDS             5499..5627
                     /locus_tag="LK694_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBK5053127.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5631..6176
                     /locus_tag="LK694_002293"
     CDS             5631..6176
                     /locus_tag="LK694_002293"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBK5053128.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6629..8320)
                     /locus_tag="LK694_002294"
     CDS             complement(6629..8320)
                     /locus_tag="LK694_002294"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBK5053129.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8740..9687)
                     /gene="arcC"
                     /locus_tag="LK694_002295"
     CDS             complement(8740..9687)
                     /gene="arcC"
                     /locus_tag="LK694_002295"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBK5053130.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9802..10821)
                     /gene="argF"
                     /locus_tag="LK694_002296"
     CDS             complement(9802..10821)
                     /gene="argF"
                     /locus_tag="LK694_002296"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBK5053131.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10912..12141)
                     /gene="arcA"
                     /locus_tag="LK694_002297"
     CDS             complement(10912..12141)
                     /gene="arcA"
                     /locus_tag="LK694_002297"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBK5053132.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12602..13303
                     /locus_tag="LK694_002298"
     CDS             12602..13303
                     /locus_tag="LK694_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBK5053133.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         3751 a         2712 c         2367 g         4504 t
ORIGIN      
        1 acttaaaaga ttcccactat cttaagtatt atagcaatat ctaactgttt ttcctatgaa
       61 atcattgttt ttttatttaa aaactgaata attttttctt tttttaattt ttaaataaaa
      121 gaaatataaa taaaaacaga ggaagcggaa tgatccgccc ctctgttttt atgattttat
      181 taaacttcca ataattcttt ttctttatca gcagcgatct tatctacttc tttgatgctg
      241 ttatctgtca atgtttgtac gtctttttct aatccgcgca aatcatcttc ggtaatatca
      301 ccattttttt gttgtttttt atattcatcg atcgcatcac gacggatgtt acgaatcgcg
      361 atttttgcat tttctgcttc ttttttcaca tctttagcta attcttttct gcgttcttct
      421 gtcagttgcg ggataactaa gcgaatgaca tttccatcat tggttggact gattccgata
      481 tcacttgcca taatagcttt ttcaacatct tgaagaatgc tcttgtcaaa aggtgtgatc
      541 attaaaacac gtgcttctgg aatttggatt gatgccattt ggttcaatgg tgttggcgca
      601 ccgtagtagt tcacagtaat acggtccaaa aggctagcgt tcgcacggcc tgcgcggatt
      661 tgcccaagtt cacgttgaag attttgtgct gcttttttca ttttttcttt tgcttctgtc
      721 ataattgcat ctgccatttt tatttccccc ttacagttgt tccgatattt tcgcctaaga
      781 ttgcacggcg aatatttccg tgttcattta aattgaagac aagcagtgga atatcattat
      841 ccatgcttag tgaacttgca gttgaatcca tgacttgtaa tcctttggca ataacttcca
      901 gatgagtcag ttcttcaaat tttactgcat ttgcatctac tttcggatca gcagaataaa
      961 caccgtctac attgttttta gccatcagga taacatctgc gccgatttct gccgcgcgta
     1021 atgctgctgt tgtgtctgtc gagaagtacg ggttgccggt accgcctgca aagataacga
     1081 tccgtccttt ttctaaatga cgttctgctt tacggcgaat atatggctct gcgatttgac
     1141 gcatttcaat cgaagtttgc acgcgtgtcg gtacacccac gttttccaac gtatcctgta
     1201 aagctaaggc attcataact gtagctaaca tacccatgta atcagcttga gcacgttcca
     1261 tgcccatttg tgctccgatt tgtccgcgcc agatatttcc gccgcccaca acgattgcca
     1321 tttcaacgcc cagctcgtga acttctttga tttcttggat gatttctttg attactggtg
     1381 gtttgattcc aaatcctgcg tccccagcta aagcttctcc actcaatttt aagactacac
     1441 gctgatattt cggtgtgacc atgttcattc ctccaatatt ttctattgcc attttaccat
     1501 acttagatga tttcgacact ctaagactgg tttttcttgt tttgcttaaa aaaagggagc
     1561 gcacaaaccg tgcgttcccg tctgttattc aacagaatta ttttttcact tggctcataa
     1621 cttcttctac aaagttatct tcgcgttttt cgataccttc gccaacttcg aaacgtacga
     1681 atgttttaac agttgcgcct tttgatgcaa cgtatttttc aacagtcata tcaggatctt
     1741 taacgaatgg ttggtccacc aaagcgattt ctgctttgaa tttcttcaag cgtccttcta
     1801 ccattttttc aacgatattc gctggtttgc cttcgttcaa tgcttgttct gtaagaacag
     1861 ttttttcatg ttctaattca gcttctggaa tttgtgattc gtttacataa cgaggattga
     1921 tagctgctac gtgcatagct acgtctcttg caactgtttc gtcagttgtt ccatctagaa
     1981 cagtcaatac ggcaatacgt ccgcccatat gcaagtatcc accaaatgca gcattgtcgt
     2041 ctttttctac aacttcaaag cgacggaagc tgattttttc tccgataact tgagttgctt
     2101 cgatcaagtc agattcaatt gtacctttat ctgttttgat tttcattgca gcttccatgt
     2161 ctgctggttt gttttcagca acaagttctg caatttcttt tactaaatct tggaacattt
     2221 cgtttttaga aacaaagtct gtttctgagt ttacttcaac gattgctgca gtgttacctt
     2281 tcacagcaac tgaagccaat ccttcagctg caatgcggtc atttttcttc gcagcctttg
     2341 ccattccttt ttcacgtaaa agatcaactg ctttttccat gtcgccttct acttcaacta
     2401 atgctttttt cgcatccatc attcctacgc cagtcatttc gcgtagttct tttaccattt
     2461 tagctgtaac gtctgccatg tttaactcct cctgtgtgtt tttcttttta aaaaaagctg
     2521 cttcaaagga gagtgaggct tgtcgcccag cctttgaaac agctccatca ttcaaaatta
     2581 ttctgctgaa gaattgtcgc cttcaacaac atctacgatt tcttcgattg atgttgctgt
     2641 gttttcttct gtgaaatctt cttcaactac ttgatcttca ccttggtttc cttcgatgaa
     2701 agcatcagcc atttttgaag taatcaattt aacggcacga attgcatcgt cgtttgaagg
     2761 gattactacg tcgatttcat ctggatcgca gtttgtatca accatagcta cgattgggat
     2821 atttaatttg tgtgcttctt gaacagcaat acgttcttta cgtgggtcaa cgatgtacat
     2881 tacatctggg attcttggca tatcagcgat accgcctaag aatttttcaa gacgttcacg
     2941 ttctttgttc aaaccagcaa cttctttttt aggtagaact tcaaaagttc catcttcttc
     3001 cattgcattg atttgtttca aacggctgat acgtttttgg atagtatccc agtttgtcaa
     3061 tgttccgcct aaccaacgat ggttaacgaa gtattgtcct gcacgagttg cttcttcttt
     3121 gattgcttct tgtgcttgtt tttttgtacc tacgaataat gctacgccgc cttcttcagc
     3181 aacgctcttc atgtaatcat aagctgcatc tactaattta actgtttttt gcaagtcaat
     3241 gatgtagatt ccgtttcttt ctgtgaagat atatttcttc atttttggat tccagcgacg
     3301 agtttggtga ccaaagtgta cgccggcttc tagtaattgt ttcatagaaa ttactgccat
     3361 gtttgtttcc tccgatttgg ttttattttt cctcttctta tcttctacct tgccggcaaa
     3421 ctttttcaag cacctaccga caatccaata agaatgtgga tttgctgatg cataaagcac
     3481 cctcagctat tatacagaca aaagatccgt atcgcaagag caaatatact tttctgctca
     3541 acattcaata gtttacctga aagataaaaa gaattcaaga aaaacagtag atttctcttt
     3601 gtttctttat tatccttttt ccataaaata attttaggta taaaagacga atattattgg
     3661 gcaatccttc ttttttttgt tatgatacat gtgaaatctt tcgtaaagga gaaagaaaat
     3721 gcgcaaagct caaagacatt tgctgattaa acaagtcatt gaagaattcc cgatccgtac
     3781 gcaagaagaa ctattaaaca aacttgggca ttatggcgtt accgccacgc aggcaacgat
     3841 ttctcgagat atccgtgatc taaaaatcgt gaaggcacct gatgaaaatg gcgtttcccg
     3901 cttcgtattg tttcgcgaaa aaggaactcc tgagactagg actgaggaag aggatcggct
     3961 ggttcaaatg attgaagaca ttgtattgaa agtcgaacga gtccactttt tgacgatcgt
     4021 caataccttg ccagacaatg ctcatttatt tgcatctgtt ttagatgaga tcaaacctcc
     4081 ctctattgtc agtacaattg ctggattcga tacgatcatg gtcatctcgg aaaatgagga
     4141 ggacgcaaaa cgagtagaag aatttcttca ccatccaact gaagtatcac ttttttaatc
     4201 agagcacgta ttcctaatat ggaatgcgtg tttttttatt ttcaatattt tccttattga
     4261 ataaaccttt acactcttta caaaaataca tcatttttta ttcaaatttt tcaacacctt
     4321 gattgaatca caataatcat catttatatt ggatgttaac aagagcagct tcttgctctt
     4381 tgtgtatttc aaaaaagaaa tgaaaggaaa tcatatgtat aaattattaa cttctggacc
     4441 attgacgact tctgaaacag tgaaaaatgc tatgctgatc gatcattgta cttgggatca
     4501 agaatacaaa gaaattacac aatggataag aaaaaaatta ttggatcttg ctcaagtatc
     4561 ggaaggaata tacacaacgg tcttaatgca aggtagcagc tccttcgggg tagaatcggt
     4621 attaagttct gtgatcaggc cagaagatac tgtactgatt tgttcaaacg gcgcatatag
     4681 acaacgaatg atcaacatgt gcgaatgtct gaaactgaac tataccgttt atgaggtatc
     4741 ggaacatcaa atcctccaag ctcatgtgat tacacagctg attgaagaga atccgtctat
     4801 ctccgcacta gcaatggtcc atagcgagac tacttccggt attttgaata atttggaaga
     4861 gattgctgct gtcacacaag aaagagatct tttgtttata gtggacgcga tgtcaagctt
     4921 tggtgggata ccgatttgtg tagccgaact agggattgat tttttgattt ccagctcaaa
     4981 caaatgtatt caaggtgttc caggcttttc ttttgtactt gctaataaag cggaactgga
     5041 aaaacgaaaa aatgttagcc gaagtttgtc acttgatcta tatgaccagt accgcgtgat
     5101 ggaaaacgag aatggcaaat ggcgtttcac ttcccctacc catacggttt tggcttttca
     5161 acaagcgctg aaggaactag aggcagaagg aggaatcgaa actcgatatt cacgttatct
     5221 cgccaataat catgtgctac gacaaaaaat gacagagatg gggttccagc ctttcattca
     5281 agaatatcag gggccattca tcacctcttt tctttaccct aaggactggc gatttgattt
     5341 taatcatttt tatcagtatt taaagcaaaa tggatttgtg atctatcccg ggaaaatcag
     5401 cgatcatgaa tgttttcgta tcggaaatat tggcgagatc tatccggaag atattttgga
     5461 actgacagca attatcaaag actataagga gaacgttaat gattaaaact gtaatttttg
     5521 attgggcagg aacaactgtg gactttggct gtatggctcc tgtccacgct tttagaaatg
     5581 cttttctaga aaaaggaacc caactgactg acaaagaaat ccgctaaccg atgggaaaat
     5641 taaaatggga tcatatccaa cagcttcttt ccctcccttc tgtcaaaaaa caatggatag
     5701 ctatttatgg acaactacct caggaaaaag aggtggagga attgtatcaa caatttgaac
     5761 attatctgtt taaagatttg gtcaagcatt ctaccttgaa acccgacaca ttggaaacga
     5821 tcgaacggtt gagaaacgct gggatcaata ttggttccac gaatggattt aatgctccta
     5881 tgatgacaat cgtagcagaa accgcaaaaa aagaaaggta ttcaccagac ttcatggcaa
     5941 cttttgaaga cgtcgaaggg tatggtcgac cgtttcctta tatgatccat aaaaacatgc
     6001 agtacttcaa aaacaagtcg gttgatgaag taatcaaagt cggagatacg gtctcagata
     6061 tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt aattgaaggt agttcgctaa
     6121 tggggatttc ttatgatgag taccaaaaat tagagatggg gaaaaagatc gtttaaaaca
     6181 gtctgttgct cttagattcc aacaagccgg cgctgatatt tgtgtaaaca atttaaaaga
     6241 gttaacagaa tacattttgt acgctgacaa gaatccagaa tgacttttat acaaaataag
     6301 aggttgtgaa aaagctgtcc tgtatcaagt aataaaaaca gccaagcaaa aatagctcct
     6361 catatttttc gtttggttag gcttattacc gcagatgcaa gcttttgaac accgtttatt
     6421 cgaaaaaaaa agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac
     6481 agtgtttaag agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg
     6541 ctcaaaaaac gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc
     6601 agaacgctga tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca
     6661 aacgaaggtc ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt
     6721 tttgttcatc atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta
     6781 atttgattgc atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg
     6841 tttcagggta tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat
     6901 agtctgctgt ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat
     6961 gtgtatattg cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa
     7021 agtcaaatgt attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa
     7081 cttgtttagc tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt
     7141 tagcagattg gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag
     7201 ttgataattt tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag
     7261 accagtcaaa gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt
     7321 cattccctac tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag
     7381 cagctaagtc acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat
     7441 tcaggtcata agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt
     7501 tttcttctaa aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca
     7561 acgaatcgaa acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat
     7621 cacggaacca ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc
     7681 aagcacgagc ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt
     7741 ataaacgaag caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt
     7801 tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga
     7861 ttggctgata accgattttt tccataatga aaccgatcga attaccgata actgtcgaac
     7921 gcaaatgtcc cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg
     7981 ttccttgatt cccgatgttg ctatccccat aatgttcttt ttctttcact actgtttgca
     8041 aaactttttt gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt
     8101 cgattttttc aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg
     8161 gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag
     8221 ctgattttgg attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag
     8281 cgtcatagac agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt
     8341 tcccttgatt gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta
     8401 gagacgagat ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga
     8461 tatcggtaga cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc
     8521 ttcattcggc ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg
     8581 ttcaacaaat cttagtctta ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg
     8641 ctagtcgttt cataaaaaaa aaatccagat ggcctaaagg agaagaaata ccacctggat
     8701 agctataaat catttcgtaa atacgaaaga cttaacaaat taatcagcta cgataatcgt
     8761 tccttcttca gaagctaaaa gattctcgat attttctaaa gaagtgatga ttgccttagc
     8821 gtttggtttt gcttcaacaa atgcaatagc tgcttctact ttcggaagca tgcttcctgg
     8881 agcgaattgc ttttcatcaa tgtattgttt catttctgaa acagtaacag tttctaattt
     8941 cttttgatct ggtttttggt aattgacata cacattgtct actcctgtca atacgatcag
     9001 caaatccgca tcgatgattt cggctaattt ttctgaagca aaatctttat cgattacggc
     9061 ttctcgtcct tctaatccag tagctgtttc tacaacagga atcccaccgc caccaacaga
     9121 gactgtgata actccttgat cgaccagcgt ttcgatgaca cgtgcttctt taatcgagat
     9181 gggtttaggg gaagcgacta cttttctcca tccacgtcca gcatcttctt tgaatgtcac
     9241 agtggagtca gcattcattt gttctttggc ttcttcttcc gtgtagaatg gaccgactgg
     9301 tttacttgga tttttgaatg aaggatcatt ttcatccaca ataacttgag tgactaaaga
     9361 aaccacatct ttatcgatcc ctttttcctt cagtacttcg cccatggcgt tttgtaacca
     9421 gtaaccgata gatccttctg tcattgcaac acatgtatct aaaggcattg ccggtgtttt
     9481 ctcagaatca gcagcttgct gctgaatcaa taggttaccg acttgcggac catttccgtg
     9541 ggagatgatc aattcatctc cttgttcgat aaatttcact aaatactttg ctgtttccat
     9601 taacgcttct tgttgggctt ttgcacttgc atccgtagac aagatggcat tgccccctaa
     9661 agcgacaact acttttcgtt tggccatttc attcgtcctt tcttcagttc taccgaattt
     9721 tctagataac aaaacgagcc agcgttttgt gattttccct caatgctgac tcgttttttc
     9781 gtgagatgaa tgtggttatg cttaaacgcg agggataaac aagttaccta atgttgctgc
     9841 catgatcgct ttgattgagt gcatacgatt ttctgcttgg tcaaattgac gagcatattt
     9901 gctgcggaag acttcatctg ttacttccat ttcagtgatg ccaaagcgtt ctttcatttg
     9961 ttcaccgtaa acagtgtttg tatcatggaa agctggcaag cagtgcaaga agatcaaacg
    10021 atctgtgttg tgtgtttttt ctaccatttc catattgatt tggtaaggtt tcaacaattt
    10081 gatacgttct tcgaatttat cttcttcacc cattgatacc catacatcag aataaaggac
    10141 gtctgctccg tctacacctt tatcaacatc gtcggtaatc atcaattgtg caccggattt
    10201 ttcagcaaag ccttctgcca ttttgacgat ttcttcttca ggttgtaatt cttttggtgc
    10261 aacgatacgc atattcgctc ctaaaattgc acctgtgact aataaagagt tagccatatt
    10321 gtttcgacca tctccgcagt aagcaacagt gataccttct actgttccaa agttttcttg
    10381 gatagttaaa aagtcagcga tcatttgtgt tggatgccat tcatcagtca agccattcca
    10441 aactggaaca ccagagaatt ctgctagttc ctctaccatt ttttgactga atccacggaa
    10501 ttcgattccg tcaaacatac gtcctagtac tttagctgta tcttctgtgg attctttttt
    10561 acctaattgg atatcatttg cacctagata ttcaggatgt gcacctagat cgatcgctgc
    10621 agtagtaaat gcagaacgag tacgtgtaga tgttttttca aataaaagcg caatattttt
    10681 tccttctaga tagtgatgtg ggataccacg ttttttcaag tctttcaagt gttctgaaaa
    10741 atcgatcaaa tattgtaatt cttcttttgt aaagtctttt tctgctaata agcttcttcc
    10801 ttggaatact gattctttca tattaaacat tctcctttag attgttttta tttaagatta
    10861 aagaggaaat cccgacggca gctgacagta agactgccgt caggaaacaa attacttcaa
    10921 gtcttcacgg acaagtggtt ggctcatgca gcgtggacca ccacgacctc gtgacaattc
    10981 acttgaattg atttctaaca ctttgatgcc atagctacga agcaattcgt tcgatacata
    11041 atttcggtta taagtgacta caacacctgg agcgattgcc aatgtgtttg atccatcatt
    11101 ccattgttct cgaggtgcaa cgatctcatc tccattacca gttgggatca gtacaagatc
    11161 atctaatcca agagcagctt tcatcgtttc gtgtaagtta tctgaatgag taatcttgat
    11221 atcatcacca tctggttcga ttgtaaagac atccatctta ccgtctttac tttgaattgc
    11281 tggatggata gtgaatttat cgtgatctac cattgtaaat acggtatcca aatgcatcat
    11341 tgcacggttg ttcggaattt tgattgccaa cactttttca aatcctgaat tttttgcgaa
    11401 taggtggcgt gctaattttt ctaaagcttt cgcattagtt cgttgagaaa tcccaacagc
    11461 tactacttta tcgcttaaga tcaattcgtc tccgccttcg atatgatctg gattttcacg
    11521 atccaaccat acttcgattc ctttatttgc aaatcttgga tgatgtttca aaatatattc
    11581 tgtaaacatc gattcacgac gacgtgcttc aaaagtcatc ttattgactg tcatcccatt
    11641 tcccattgat gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt ccatgtaaaa
    11701 tggatagtca tcgtctgctg acagatcata tagactgcta gaacgtactt cgatatcttt
    11761 tgtacgaacc ccagccatga ttttatctac catttcttgc gtttccattg atagaaggaa
    11821 ctcatgaaga ccctctctta cagcattcga tgcaatatga gattcatcca acattttatt
    11881 caagaattgt tctttgacat tgcctgcgtc aatcgcttct gccgctaatt tttccaagta
    11941 aagagtctcg acgccttcat tttgcaatgt cttcgcaaag ttgtcatgtt cttcttgagc
    12001 aatcggaaga tatggaatat catcgaacaa cagacgatcc ataatatctg gtgtcaaatt
    12061 ttccacttct tgtccaggcc gtttcagtaa aactgtcttt aatttcccta tttcagagaa
    12121 aacgtgaata ggtttatcca tgttgtgttg cctccttctt tttgattaca tgtataggat
    12181 atcctccttt tgtaatcact ttcataattt tgtctcattc aaaatgagac attcctcaca
    12241 ttaatattcg ataattaaat aattaaacgt ttttctattt ttaagcgtaa tttattcatt
    12301 ttttattctg tatacacttt caaaaaaata tttattcatt gaaaaaattt ttttgaataa
    12361 attaaaaaaa taaattatat tatcgttgtt agcgtttact atatgtatta ttgttgtttt
    12421 ttatacacaa atcattcgct tcaaaacaaa ttattcaata ccattgtata ttttttacat
    12481 aataacaaat tatatttgta aagtattaaa acttgtttct aatgttggta tgttaaaact
    12541 ttacaaattc tttaaaaaaa gtgacaataa atatagtgaa caataaggag aggacttttt
    12601 aatgcgtgtt aataaaatcg attttcactt ttcaaaactc agatatcaac ctgattttga
    12661 acatttctcg gatgaagagt tccaacagat caaggaacat actgttcttc gttcttataa
    12721 aaaaggacag atccttttcg acgatggtga tcccagacaa cggttgtata tcttgaccaa
    12781 aggactcgtt cgtttagaac gttatgatga aagtgcaact tattactact atgactatgt
    12841 gaatacaaat aacctttttc ctatgtcagg aatgtttgga gaagagacct acacttatac
    12901 agctcaagcg atgactgata ttgaaacatt ctaccttcct gtttcctact atgaaaaatt
    12961 agcacgaaaa aatcccgaac agttgatcta tatgatacaa aaaatttcta aagtagttga
    13021 aatacatgaa ttgcgtatcc aaatcggatt gacctctagc gcctttgacc gggtaaaaca
    13081 aaatttattc attttgaaag aagaattggg cgtaccaaca aaagatggtc atatcaccat
    13141 tccttatccg atcaccttaa aagaattagc agtgaatagc ggcacgacac gtgaaactgc
    13201 tggacaagta atcaaacaac tacgtgattc cgggctgtta gattatgaca aaaagcgttt
    13261 tactttctgt aagacagcta ctactccagc tatgagagaa taacgcgaac aaaaataagc
    13321 agtgaccctg ataa
//
LOCUS       DAFBQH010000078        13096 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.080, whole genome shotgun sequence.
ACCESSION   DAFBQH010000078 DAFBQH010000000
VERSION     DAFBQH010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.080"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            149..1072
                     /locus_tag="LK694_002299"
     CDS             149..1072
                     /locus_tag="LK694_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBK5053134.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1078..2331
                     /locus_tag="LK694_002300"
     CDS             1078..2331
                     /locus_tag="LK694_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBK5053135.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            2465..3811
                     /gene="gor"
                     /locus_tag="LK694_002301"
     CDS             2465..3811
                     /gene="gor"
                     /locus_tag="LK694_002301"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBK5053136.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRCLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(4625..5578)
                     /gene="menA"
                     /locus_tag="LK694_002302"
     CDS             complement(4625..5578)
                     /gene="menA"
                     /locus_tag="LK694_002302"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBK5053137.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(5591..6694)
                     /locus_tag="LK694_002303"
     CDS             complement(5591..6694)
                     /locus_tag="LK694_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBK5053138.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(6784..7740)
                     /locus_tag="LK694_002304"
     CDS             complement(6784..7740)
                     /locus_tag="LK694_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBK5053139.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            8021..9955
                     /locus_tag="LK694_002305"
     CDS             8021..9955
                     /locus_tag="LK694_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK5053140.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            10412..10828
                     /locus_tag="LK694_002306"
     CDS             10412..10828
                     /locus_tag="LK694_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBK5053141.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            10842..11408
                     /locus_tag="LK694_002307"
     CDS             10842..11408
                     /locus_tag="LK694_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBK5053142.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            11431..12417
                     /locus_tag="LK694_002308"
     CDS             11431..12417
                     /locus_tag="LK694_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBK5053143.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(12572..>13096)
                     /gene="scm"
                     /locus_tag="LK694_002309"
     CDS             complement(12572..>13096)
                     /gene="scm"
                     /locus_tag="LK694_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBK5053144.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         4075 a         2374 c         2707 g         3940 t
ORIGIN      
        1 caatctcttt ttatatttgg acagaatata tcgaacttta ttttgatttt ttgtgaaata
       61 attttcaaaa atccgacttt aaatagaaga agtattggaa agtttttaga gctatgctac
      121 tattatgaag ttgtaggagg agaaaattat gatcgaactc ataaccacag tagaaactat
      181 tgaacaagca gaacagttgc ttgctgcagg tgcagataca ctatatttcg gtgaagagca
      241 atttggattg cgtctgcctg cttcatttac acgagaagaa cagcgacaat tggtagaatt
      301 agcacacaag tatggtaaaa aagcgaatat tgctgtaaat gggatcattc atcctgaaaa
      361 aatgaaaaat gtacctgaat atttggcttt tttaaaagaa ataaacgtag atgtgatcac
      421 agttggtgat acaggtatta tttatacgat gaaaaaaaat cctgaattgc gtataccgta
      481 tatttatgat gctgaaacat tagtaacgag cgcacgtcaa atcaatttct gggcgaaaaa
      541 aggcgcagtt ggagcagtac ttgctcgtga agttcctttt gaagagatga aggcgatgga
      601 agaaaaactc gatattcctg tagaaaccct tgtgtatggt gctacatgca tccatcaatc
      661 caaacgtcca ctgctacaga attactataa ctatacgaaa caagacgaac aaaaagatcg
      721 tgaaagaggt ctttttatct cggaacctaa aaaagaagag acacattatt ctatttatga
      781 agatagtcat ggcacacata tttttgccag caatgatctg aatctctcga atgaattgaa
      841 tgaactttat cagcatcatt actgtacatg gaaattggac ggattattta ctccaggcga
      901 aaaattcgta aacatcactc gactattcgc cgaagcaaaa aaacaaattt tagaaggtag
      961 ctgggatcaa gaaaaagcgg aaaaaatgac tagcgaaatc agtaaattgc atcctgaaaa
     1021 ccgtgaatta gatacaggat tcttttatat tgatccttct gctatcaaat aaggagtatg
     1081 atgaaagaaa tgacagtaaa aagaaaattg aaacgaccag aaatcctagc gccagcagga
     1141 acactggaaa agttaaaaac cgcgatccat tatggagcag atgcagtata tattggcggg
     1201 aatgcatatg gtttaagaag ccgtgcaggt aattttactt atgaagaaat ggctgaagga
     1261 gttgcttttg caaaagaaca tgatgcgaag gtatacgttg ctgctaatat ggtcacccat
     1321 gaaggaaatg aagatggagc aggggaattt ttccgcagtt tgcgtgatgt cggtatttct
     1381 gcagtgattg tttctgatcc agctttgatc gaaatttgtg cgacagaagc cccaggtctg
     1441 ccgatccatt tatccactca agcttcagca actaattttg agacattgga attctggaaa
     1501 gaagaagggt tggaacgtgt cgttttagct cgtgaagttt caatggagga agtggcagag
     1561 attcgtaaaa atacggatac cgaaatcgaa gcatttatcc atggtgccat gtgtatctct
     1621 tattctggtc gatgcacatt gtccaaccat atgtcgatgc gggatgcaaa ccgtggcgga
     1681 tgttctcaat cttgtcgctg gaagtatgga ttgttcgata tgccttttgg tcaagaacga
     1741 aacagtttaa ctgatacagg cgaagtggaa gaagaattct cgatgagtgc agtggatatg
     1801 tcaatgatcg aacatattcc agaattgatt gaaaacggcg tagacagctt caagatcgaa
     1861 ggaagaatga aatcgatcca ttatgtatct acggtgtcaa atgtctataa agcggctgta
     1921 gacagttata tggaagatcc tgaaaactat gtctgcaagc aagaatggat cgacgagtta
     1981 tggaaagtag cacaacgaga attagctaca ggtttttact acaatacacc aactgaaaac
     2041 gaacaattat tcggtgaacg tcgtaaaatc ccagtatata aatttgtcgg tgaagtatta
     2101 tcttatgatg aggcaacaca gattgctacg atacgtcaga gaaacttgtt ttctgtagga
     2161 gatgaaatcg aattttatgg tccaggattc agtcatttca gtcaaaccgt tgaagttatg
     2221 cataatgaag aaggcgaatc cattgaccga gcaccaaatc caatgatgat tttaactatg
     2281 ccagtcgcac agccggtgaa acctggagat atgatccgaa aacgtaaata aggaaaaagc
     2341 tgatatatcc ctgttttaag catcttcgca tacaaaattc aagaaaagtg ttacacttgt
     2401 tttctgtaga aaaaggatcg ttaaataaag gttttgattg atctcttcaa taaggaggaa
     2461 caaaatgcaa atatatgatt acatcgtaat tggtggcggc tctggcggta ttgcttcagc
     2521 aaacagagca gggatgcatg gcgcaaaagt tttgctgatt gaagcgaatg aattgggcgg
     2581 aacatgtgtc aacgtcggtt gtgtacctaa aaaagttatg tggcaagcaa gtacgatttt
     2641 ggaaacaatt gaacgagatg cctcaagtta cggtatccaa gcagatttaa aaaatgttga
     2701 tttcgccggt ctagtagaaa gacgagaaaa atatattgat tttctacacg gtgcttatca
     2761 aagaggatta gatagcaatc atgtcgaggc tataaaaggt tacgctactt ttatcgatag
     2821 tcaaacgatc gaagtaaacg gtgaaaccta tcgtgctcct aaaatcttga tcgctactgg
     2881 aggccgtgca agtaaaatga ccattcctgg tggagaatac gcaattgatt caaatggatt
     2941 ttttgaatta acagagttac ctaagagagc aatcgtttta ggtgctggat acattgcggc
     3001 tgaactttca ggtgtatttc gtggtttagg tagtgaagtc atgtgggcat atcgaaaaga
     3061 acgaccactt agaacgtttg ataaaatgct ttcggacaat ttgatccaaa tgtaccaaga
     3121 agctggaata aaaacttacg cacatcatat tgctaaagaa attactaaaa acaatgacga
     3181 atatacggtg ctatttgaaa atggtgaaac cttaactggt gattgtgtac tttttgccgg
     3241 tggacgtgta ccgaataccg aaaaattagg tctggaaaat acaaatgtgg aattggacaa
     3301 aaaagggttt atcaaagtag ataaatacca aaatacaacg gatgaacata tttttgctgt
     3361 tggtgatgtg atcggtaaat tagatctgac tcctgttgca atcgcagctg gtagatgttt
     3421 atcagaacgt ttatttaatg gaaagaaaga ttcttatctt gactataaac ttgttccgac
     3481 agtcgtcttt acgcatccac caattgcaac aattggtttg acagaagaag aagcattgga
     3541 aaaatatggc gaaaatgaat tgaaagttta tcgttcacgc tttaccccaa tgtactttgc
     3601 attaaatgat tatcgccaaa aatgcgaaat gaaattgatc tgtgtcggaa aagaagaaag
     3661 aatcgtcggc ttacatgcta ttggtgttgg tgtcgatgag atgctgcaag gttttgccgt
     3721 ggctatcaag atgggtgcaa ctaaagaaga ctttgacaat acagtagcca tccatccaac
     3781 aggagcggaa gaattcgtaa cgatgcgata atttaatatt aacagttcca acagcttttt
     3841 attgaacaaa ccatgtaaaa ccagaaaatc agtatactga ttttctggtt ttttgttttt
     3901 tagttattta tgtaagattg cttcgataat tctcaaaacg aaataagtag ggaagttgga
     3961 gattttgatt atcttcgtaa ctgacgaaga gtaaatgaat ccatttggta tgcactctta
     4021 agttcaaaat attggtatcg atgtttaaaa gaaatagaaa tgtatcgaag aacagatgtt
     4081 taaaaaagtg tgactttgta taaataaatt agaagaagca aacgaggatg ttaaaagaat
     4141 accatatagt taccaaacca agaaagttct cgtcaagttc gctggtacga atcaaatcaa
     4201 gtgaaaaaag ggactgtgaa aaaaattgtg tcatagttag tcactttttc cgaacaaata
     4261 gtgttcaatc agctgtcctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt
     4321 tttccatttg tttctctaat actcaggaca aaacactgat tttacaacct ctgataaacg
     4381 gtgttcaaaa gccagtttct cggtattaag ccaaatttat gcaaaaatgt ggaaagccat
     4441 tttcacaaaa tttttcttaa tactcgaaac taagcgactt tttcacaacc tcctgataaa
     4501 cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tataaggagc
     4561 tatttttatt tggctgttct tattacttga tgcagggcag ttttttcaca acctcttcat
     4621 gctgttaaat ggcttgcata atgattgtta gtaatagccc caatacataa ctggcattga
     4681 ataagaccat atttttgatg gaatagctga aactgatagg ctggggtaac gtttgcttga
     4741 attgatccaa atgcttcttc acaattggca aggtcaagaa ggtgaccaag atcggccatt
     4801 catagatacc gctcaacaac cccattaaaa tcacgaaata gcaagcatac atcaatagct
     4861 ggaataagac tgttcccgca ggacgtccga tataaaaaac tagtgtatag cgatgatttt
     4921 caatatcacg gtcaagatcc cgcaaattat tagccaacat gatattggca atcgtaaaca
     4981 ccaagggtaa ggaagctaat acgattgctg ctatagccca aagatttcct accaaatcaa
     5041 attgtccaga tgaaaagttg actgtcagat catagacatc tgaaccgaat gtattgacat
     5101 aaacgaccat ggcaaaaatt ccaaggccca tagtcacccc actgaaaatt tcccctaaag
     5161 gcattcgaga taaaggaatc ggtccgaatg tatagaatac tccaataaaa caacagataa
     5221 atcccatcaa aagtaaaagc caacccgtat tgatcgttag aataacgccg ataacaaaaa
     5281 caaatgcaat catagctaaa atcatgtttc gaatcaatac aggagataga tttctgcggc
     5341 caatgacatt ttcttcatac ttatatactt cagaatgagc cttttcataa tccatgtaat
     5401 tattgatagc ggtagtcgct aaatcaaaaa ctagcattcc aataaaaaat aaaaaagtgt
     5461 ttagccaatg tatctcatga aaaaaagcga tagagaataa tacccctacc gcaaaaggaa
     5521 atacactagc cacctttgtt cgtatttcaa ctacttctaa aaagattttt agtgtcatgg
     5581 ttcgtactcc ttattttagt tcagaacggt cacccatcgt gtaatgggag tcctccgata
     5641 atttgaaggt gtctttgatt ggatcggtca catatacttt atcgtctttt gtcacgaaga
     5701 tcgcttccgt cccgttattc aattcgcttt cgacgtattc cagtcctttt ttcacaccca
     5761 ttgaaaagac tgctgtagac aacccgtcac catcaattga tttatctgtt atgatcgtga
     5821 cacttgcgat atcattgtcg aacggataac ccgtttttgg atcaaacaaa tgatggtatt
     5881 ttttgccatc tacttctaaa taacgctcgt aaattccaga ggtaactaat gttttgttcc
     5941 gttctttaat agaacctaaa acagatccgc gagccatgtt cggatcttgg atgccgactg
     6001 tccagtcttg gttttcgcct cttgggctgt ggcctaagac atagacatta ccgcccaagt
     6061 caacgatggc agtggtcact ccttgttttt tcaagacttt cactacttcg tccgtaatgt
     6121 aacctttggc gattgcacct aaatcgatga tcattccttt ttcttttaga taaacagttt
     6181 tttctttgtc atttagttcg atcttatgat aatccactaa tttcaatgcc tgatcgattt
     6241 cttcttggga aggttttctg gcgtcatcaa aaccgattcg ccataattgt gtgattgcac
     6301 caatcgccat atcgaatccg ccttgagaat cttggctata ttcgtaggcc cgcttgacta
     6361 acgtgtagac gtcatcagaa acttttactg gtttgatccc agcttgctca ttcacttcat
     6421 cgatctcaga acctttttga ttgatcgtga ttttatcacc aagttctttt actcgatcga
     6481 aggcaggttt caatgctgat tctttgcctt catcatacac acggatacga acgtaagtcc
     6541 ctaacaagaa ctgttcatca gaatatggat ctttcaatat cttggtagct gctgaacttt
     6601 cagtggttgt tttatttgct gagttgctgc agccagttag caccataaaa gagatgccca
     6661 gtgtgattaa ggaaaatagt agttttttcc gcatcttttt cctctcttca ttttgaaagg
     6721 tgagacgact gcctaatctg gctatcgtcc caccctccaa cttttattag tatgattatg
     6781 tgattatttt tctgttacga tattatctac ttcgattgtt tgagtgtcgc ctttttcagc
     6841 tgcattcact aattgttgtg cgtacatgat gaatgaatgt gagctgtgtg tagcacctgt
     6901 aacaacttct acattagcag gtgagccatc ttcttcgccc attgctttga cgaattcgtc
     6961 gttcagagta gggatgtaag tttttggttc tgtaccgctt tttgctttca tattttcatt
     7021 atattctgta tcatcttgtt ttgatttacc gtctttgtcg atataatcaa agtttgattc
     7081 agttactttg ccgccagcaa ctgtcatttc gaattgcaca cggtagccgt tagagtagtt
     7141 cttttcttca agcttgtaag tgccgtcttt caaatcagca ccattgtcga tttcgattgt
     7201 atctgtgtta ccagcttgag cagcttggat caattgttgt gcgtagtttt ggaagctttc
     7261 agatgagtga gtagctccag ttacgacttc cacaccgctt gcgctttgtg cttttaagaa
     7321 ttggtcattc aactcaggaa tgtatttttc tgggtttgtg ccagattttg cttccatgtt
     7381 tttgttgtat tctgcatctt ttgtttttga ttcgccgttt tcattgacgt tatcatattt
     7441 tgattcagtg attttgccgt cttttacaac catttcaaag actgcacggt aaccatttga
     7501 ataatttttt tcttctaatt tatatgtgcc gtcttgcaaa tctccgccag ctacaacttt
     7561 tgcagatgat tctgttgtgc tttttgcagt cgaagattgt gctgtagatg attcagaaga
     7621 agagtttgag cttccgttgt catctgcgcc gcaagcgcct aacactagag ctgaaaatgc
     7681 taatacggca aatccagtta caaaacgttt tttcttcatt ttaatacccc accattccat
     7741 ttctagtcca caatttttct ttgtgattca ttatacattc taatcttttt agcctataag
     7801 tcaactattt ttgataaata acccatgtaa tatttcaaac gttttctttc agcaaatttc
     7861 gtgaaaaaac gctttatttt aaaatttgaa aaaaatccaa aaggagttag acaaaagaac
     7921 agaacatcta tataatatat ggagattgtg taataagtat caaactaaaa gaattatcat
     7981 atattcatta tatagatagt ctataaggag cggatgatca atggcaaagc aaaatattgt
     8041 cgttgtagga gcaggatatg ccggtgtttc agctacaaaa tacttggcaa agaaactgaa
     8101 aaaagaagat gtagcaatta ctctgatcga tcgtcattcg taccatacta tgatgaccga
     8161 gttgcatgaa gtagctggcg gacgtgtaga accagaagca atccaatacg atctgcagca
     8221 tttgtttgct cgtcaaaaga atgtacaact tgtcacagac actgtaatcg gtatcgataa
     8281 agaacgaaaa atcgtcaaga ccaaattagg ttcctatcct tttgatcagc tgatccttgg
     8341 aatgggcgga gagccgaatg actttggtac acctggtgtc aaagcacacg gattcaccct
     8401 ttggtcattt gaagattctg taaggattcg ggaacatatt gagaagacag tagcaaaagc
     8461 tgcattggaa ccagatgatg aactgcgtaa agccatgctg actttcgttg tctgtggttc
     8521 tggatttacc ggtattgaaa tgattgggga actgatcgac tggaaaaatc gcctagccaa
     8581 agattataaa ttagatccaa acgaaatcac actgatggtc gtagaggcaa tgccgacgat
     8641 cttgaatatg ttggatcgaa atgatgctgc aaaagctgaa cgctatttgc gtaagcacaa
     8701 cgtggaatta ttattgaatg caccaatcgt agaagtggct gctgatcata tcaaattgaa
     8761 agacggacgc aacattccga ctcacacact gatttggaca gctggtgtca aaggaacttc
     8821 tgacgctgct gactttggat tagaatcagc acgcggacag cggttgatcg ccaatgaata
     8881 tatgcaggca aaaggctacg aagataaaaa catctatatc attggtgatt tagtctttta
     8941 tgaagaattc ccaggaacac cgacaccgca gatcgttcaa gctgctgaac aaacaggaca
     9001 cacagccgca gcgaatatcg tggcttcgat caaaggcgga gaaaagcata aatttaaagg
     9061 aaactatcaa ggatttatgg tttctattgg tgccaaatgg ggggtagcta acctatttga
     9121 taagatccat ttaagcggat ttttagctat cgtaatgaaa catatcgtga atttgaaata
     9181 cttctttgat attcgttctg gttattatat gttccaatat ttaatgcatg agtttttcca
     9241 tatcaaagat gatcgcaacg tgactcgagg acattcttct cgttacggca atgtcttatg
     9301 gagtgtgcca cttcgtattt tctatggttt agtttggttc gttgaagctt ccaaaaaaat
     9361 cattgggaat ggtgaagtgt tcaaaccaag tacttggttt ggtgaaggat catggttcac
     9421 tggaaacagt gtgttccctt ggacatggga atacaatgta acagatgttg ccagtggtgc
     9481 ttctgctgct ggtgatgcaa ccacagctgc cagcggagca ggggcaagtg ccggagaagc
     9541 agcgactcaa gctgcacatt ttggtttgag ttatgcatac ggtgaagaac caatggctgt
     9601 gattgagaag tcaccagact ggttcacaaa gatcatgcaa gtgatcatgc caaacaatga
     9661 tgtagcaatg ttcttccaaa aattcatggt attctttgaa attgccttgg cattagcatt
     9721 gattgctgga ttattcacat ggctatgcag tgcgacaacg attgtattgg tgatcacgtt
     9781 ctgcttatca ggcatgtttt actgggtaaa catttggttc atttttgttg ctttcgcatt
     9841 gatgaacggt tccggacgtg cgattgggtt ggatcgctgg gtaattccat ggctacaacg
     9901 tacactaggt aattggtggt acggtagacc aagttcccag tacggcaaga aataaataga
     9961 taaagaacgt tatttgaatg caagatttca atagaaaggg gctgagacaa accgtcacag
    10021 cctccttttt atgtaaaatt aaaggaataa gatgcccgag ataagcctcc atgagcaaga
    10081 cacaggtgtc acaacctctg ttacaacctc ttgttataaa cggtgttcca gaagtagctt
    10141 cttcgaaaaa taagccgaaa tttcacaaaa acttgaaaag taattttcgt aaattccttc
    10201 ttatttcttg aagttgccca cttctgtcac aacctcttgt tataaacggt gttccacctg
    10261 ctgcttctcg gtaataagcc caaagaatta gaaaacatgg aaagctgttt tctattcttc
    10321 gttcttatta ctcaaagcaa gacacaggtg tcacaacctc ttgtttttat gctaaaattg
    10381 ggtgagttaa tctggaaaaa ggagaacaaa aatgagcctt aaagaatttg ctgaaaaaag
    10441 tctgctcaaa ccttgggatg tttttattat tttgcttttg actgttcttt cttttttacc
    10501 agtcatgatt tttggtcttc agcaaaacac cgcaacagca gaaaaagaag cggtactgcg
    10561 tgtggatggc acagaaatca aaacattccc tttgatggct ggagaagaga gcaacacata
    10621 tacttacaaa gatgaacacg gagattataa cttgatcgag atagatggcg atcgagtacg
    10681 gataaaagaa gcagattgcg atgaccagat ctgcgtacgt cgaggctggg caacgaaaaa
    10741 tggcgaaacc atcgtctgtt tgccacataa attagtgatc gaagtccgtt caacagatgg
    10801 aggtgacgaa gacagtctga tttattagac cttcacacac catgagtaaa ttacaaaaaa
    10861 tgatttatat ttcgctactt gttgcacaag gagtgatcat tggtttattg gaaaatatga
    10921 tcccttatcc tttttctttt gcgcctggtg caaaactagg cttagccaat ctaatcacga
    10981 tcgttgccat ttttaccatg aaaaaacaag acagcttctt actactttgg ctacgactat
    11041 tcctgactac tttgctaggc ggaacgattt ccacattcct ttatagtatg agtggtgctt
    11101 tgcttagtta ttttggaatg ttattggtca agcaattagg accaaaacgt gtcagtatca
    11161 tcggaattag tgcggccggc ggtttcatgc ataatgtcgg acagcttttg atggcttctt
    11221 tcattgcaca atcatggaca gtcatgttgt acttgccagt cctttcagtt ttagggattt
    11281 tatcagggat tgcaatcggg atcgcagcga attatctttt acgacatgtg cagacacttc
    11341 aacgattcca aaaagattat gagcagggaa ccaaacaaaa atggagccca atgtttacca
    11401 aaaaataatc gtcacacaag gagttttttt atgaagctac atccaatgtg ggaaaactat
    11461 cctgctttaa gtaaagaatt gacagaaaca ctaaaacgga tggaagaaac tgtccgcctg
    11521 aaaaacaaac ctgtcgaaca agcaatcaaa gaaacgatcc atgccggcgg aaagctgttg
    11581 cgtcctgcct atcagctatt gttctcacaa tttggaccag agcaagatcg agacaaagcc
    11641 atcgctttgg cagcttctat cgaaatgctc catacggcca cactgatcca tgatgatatc
    11701 gttgatgaag cggatctgcg acgtggtcag cctaatatcc gtagtcagtt tggtaatgtc
    11761 gtttctgtct atgcaggaga ctatttattt gtctgctgtt ttaaactatt gtctgattac
    11821 tccacttcct tgaaaagtct ccaattgaat tctcgcagta tggaaaaagt attgaatggc
    11881 gaattagggc agatggatga tcgctataat tatgaacttt ctgtaaaaga atatttggaa
    11941 aatatttcag gtaagacagc tgaactgttc caattaagtt gttctgttgg cgcttttgaa
    12001 agtggaacaa gcgagcgctt cgcaaaaaaa gcaggagata tcggactttc tatcgggatg
    12061 gcctttcaga ttatcgatga tattttagat tatacaaaag agagccagga aatcggcaaa
    12121 cctgtactag aagacatgcg tcaaggggtc tattctttac cgttgatcta ctctttgcaa
    12181 aaaaacaaac caaagctact accttattta gagaagaaag cggcaatgac agaagaagat
    12241 gtcgacgccg tacgcaaaat cgttgaacat acaggaggcg tggaagaagc taggaagtta
    12301 gcggcctctt acacagaaaa ggctttgaag gaaataaaga aattacctgc cacctctctt
    12361 cgaacaaaag agaatctctt ttcccttacc caactgattt tggatcgcaa agactgaata
    12421 gggaaaggtt cgatacgtaa ataaatttct cagaaggaaa tctacgtgtt ttttgacaaa
    12481 ttgaattgta gttcttgtat ttatgataaa aagcaaaaat aaagagcaac gtccgttagc
    12541 gacgttgctc ttttttatct tgaatatttt tttacctttt cgatttctta taactcataa
    12601 aagtgattct taaaccgatt acaagtaata taacgcctgc gagtgttatc caattattcg
    12661 attgacttcc agttttagga agtgctcttt ttgatttgtt tccattgtca ttgcttgttt
    12721 tattagttaa gttaaagttc gtcctgttat ccgaacttgt ttttgagaag gagacacttg
    12781 catgacgtgt atcgcttgtg gtagacgacg tgtctgtact atcgcttgaa gttgatgaat
    12841 ctgagctatc acttgtggta gaggatgtat ctgtactgtc acttgaagct gacgtgtccg
    12901 tgctatcact tgtagtggta gatgaatctg agctatcact tgtggtagag gtagtatctg
    12961 tactgtcact tgaagctgac gtgtccgtgc tatcacttgt tgtggtagat gtatccgtac
    13021 tgtcgcttga agctgacgtg tccgtgctat cacttgtggt agaggtagta tctgtactgt
    13081 cacttgaagc tgatgt
//
LOCUS       DAFBQH010000079        12808 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.081, whole genome shotgun sequence.
ACCESSION   DAFBQH010000079 DAFBQH010000000
VERSION     DAFBQH010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.081"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(283..1314)
                     /gene="queA"
                     /locus_tag="LK694_002310"
     CDS             complement(283..1314)
                     /gene="queA"
                     /locus_tag="LK694_002310"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBK5053145.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKIGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1507..2682
                     /locus_tag="LK694_002311"
     CDS             1507..2682
                     /locus_tag="LK694_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK5053146.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESDTPEESKEV"
     gene            2686..3327
                     /locus_tag="LK694_002312"
     CDS             2686..3327
                     /locus_tag="LK694_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5053147.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3324..4241
                     /locus_tag="LK694_002313"
     CDS             3324..4241
                     /locus_tag="LK694_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK5053148.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYDQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVNNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4238..4909
                     /locus_tag="LK694_002314"
     CDS             4238..4909
                     /locus_tag="LK694_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5053149.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATDGTSIILAGALPTALMVIVTDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5105..5939)
                     /locus_tag="LK694_002315"
                     /pseudo
     CDS             complement(5105..5939)
                     /locus_tag="LK694_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016619675.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6140..6406)
                     /locus_tag="LK694_002316"
     CDS             complement(6140..6406)
                     /locus_tag="LK694_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBK5053150.1"
                     /translation="MSNYTVAIKNSATVDLRKIKQSNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6406..6669)
                     /locus_tag="LK694_002317"
     CDS             complement(6406..6669)
                     /locus_tag="LK694_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBK5053151.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSGFTNIDDIDWEAL"
     gene            7144..7329
                     /locus_tag="LK694_002318"
     CDS             7144..7329
                     /locus_tag="LK694_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK5053152.1"
                     /translation="MALNYGNKKWSLDEAEISTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7471..9294)
                     /locus_tag="LK694_002319"
     CDS             complement(7471..9294)
                     /locus_tag="LK694_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBK5053153.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9891..12596)
                     /locus_tag="LK694_002320"
     CDS             complement(9891..12596)
                     /locus_tag="LK694_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBK5053154.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPILQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT         3689 a         2654 c         2414 g         4051 t
ORIGIN      
        1 tatttttctt ttgtgagaca attttcgtta aaagtaactg ataagtctag aatatctaat
       61 gtttctttga taaggtgagt gtaagacatc ttgattcctc cttttgggtt ggttcgccaa
      121 attaattcta aaggacttca aggtgtcttt tctatatgtt ttggaaaata aaaaagatgc
      181 tggtgtcatc caccaacatc aaaaagtata gagctctttt tctttgtcat aaaatctttg
      241 taaagctttt ttgttattca aaactagcgg cttttcataa ccttatttca cgaacatcgc
      301 atctccaaaa ctaaagaaac gatagcgctc ttcaatcgca tgctggtaag cagataaagt
      361 caattctctt cctgcaaagg cactaactaa catgactaaa gtcgattttg gcaaatggaa
      421 attagttgag aaagcttcta ccactttgaa ttcatagcca ggcgtaatga aaatatcagt
      481 ccatccgctg tctgctttga tttctccatt aaatttcgtt ccaatcgttt ctaatgttcg
      541 aattgaagtt gttccgacag ccacgatttt cccgccattt ttacgtactt ggttcaatcg
      601 ctcagctgct tcttccgtca aacggtagaa ttcactatgc atatggtgtt cttcaatttt
      661 atctacgctg acaggtcgga atgtacctaa ccctacatgt agtgtcagat agactaattc
      721 aacacccttt gcttcgatct catcaagtaa ttctttcgta aaatgcaatc cagctgttgg
      781 cgctgcagca gaaccatttt ctttcgcata gacagtttga tagcgatctg ggtcttccag
      841 acgttctttg atatatggag gcaatggcat ttctcccaat gattcaagaa tctctagaaa
      901 aattccttca tactcaaagt caatgatccg tccgccgtgt tccaattctt ctttcactac
      961 cgcttgcaat ctgccatcac caaaagagat tcttgtccca actttggcac gtttcgcagg
     1021 ttttatcaat gtctcccacg tatctccttg tgtattcgtt agaagaagga cttctaaatg
     1081 cgcaccagta tctggttttt ctccatataa acgagcaggc agaactcgtg tattattcat
     1141 aactagcgca tcaccaggct gtaactcttc aatgatttca tggaaatgtt tatcttccat
     1201 ctctccaatt ttccgatcca aaacaagtaa acgagaatga tcgcggtctt ttaatggtgt
     1261 ttgtgcgatc aactcttccg gcaagtcaaa atcaaaatct tctgttgtta acatatcgtc
     1321 actcactttc tatcatgtgc ttcatcattc tatcatgttt ttcattctgt tggcaaaatc
     1381 agcacttttt cacttctatt ttttgttttc ctaaataaaa aaactaccct atttgtattt
     1441 tttcattgtt ttaggcatac tagtaataga tagttcttca ttgaaaatag aaaaagaggt
     1501 ggcaccatga ttgaatttca aaatgtgtca aaaatttata aaggtggaaa agtcgctgta
     1561 gaggacgtca atctttcatt tgaaaagggt gagttcattt gttttatcgg tacgagcgga
     1621 agcggaaaaa cgacgacgat gcggatgatc aaccgaatga ctgatccaac acaagggaaa
     1681 attttgattg atggaaaatc catccaagaa attgatccag tggaattgcg acgtcagatc
     1741 ggctatgtta tccaaagtat tggattgatg cctcacatga cgatccgtga aaatatcacc
     1801 cttgtccaaa aattgttgaa agtcagtcaa gaagaacgga ataaaacagc tgaaaaaatg
     1861 attgatcttg tggaattgcc ccgtgagatg ttagatcgct acccacatga attatctggt
     1921 gggcagcagc agcgtattgg agtcgttcgt gcccttgcag cgaatcaaga tatcatcttg
     1981 atggacgagc cattcggtgc gcttgatccc atcactcgtg attctttgca agaccttgtc
     2041 aaagatttac aagagcgctt gggaaaaacg atcgtatttg taacccatga catggatgaa
     2101 gcgattaagc tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat
     2161 acaccagaaa atatcttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa
     2221 gatcgcctac tacaagccaa gccaaatgcg acaagagtcg gagaagttat gctaaataca
     2281 gcaatcacga ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa
     2341 cgtgtagata cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta
     2401 gaaacgttga ataaaaagcg aggaaaagta tccagtgtcg gcgacatatt aaataaacat
     2461 gttttttacg taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc
     2521 ggactgaaat acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt
     2581 gcatcattag tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat
     2641 gttgcagagt cagatactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt
     2701 catcagcacg gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcgggactt
     2761 gctttgatca ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca
     2821 aaagctgcca cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct
     2881 ttattggcat tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc
     2941 tttatttatt cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat
     3001 tggaattatc gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc
     3061 gttgaattgc ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac
     3121 gtgattgcat gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt
     3181 tttagtggac tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaacg
     3241 attttagcac tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc
     3301 gcacttagag aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct
     3361 ctcttcttat acttgcgagt tgttctttcc ctggtcttgc cagcaataca gatgaagaca
     3421 ccatttcgat cacaggtgga atcacttcag aagcacagat cttagcaagc ctagttgccg
     3481 gaatggttga acattataca gataaaaata ccgcaatcat caacaatctg gctaccacaa
     3541 ctatcaacca tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag
     3601 gaacagattt aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg
     3661 ctaccgtgaa agatgaattt gagaaaagat acgatcaaac gtggttcccg tcctatggtt
     3721 ttgaaaatac gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catttatcta
     3781 aagtcagcga tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga
     3841 tcaatcgtaa aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt
     3901 cgatattacc tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata
     3961 ttgttcttgg ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag
     4021 atgatcgtca attcttccca ccatatgatg ctgctccagt tgtaaacaac aaaatattag
     4081 ctgatacgcc tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa
     4141 aaatgcagga attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac
     4201 aacgtttctt acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa
     4261 tgtctttcaa caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt
     4321 tatccgtcac ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc
     4381 ggttgggatc atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa
     4441 tatcatccag acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct
     4501 gggagtcaat gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa
     4561 tacgtatacc gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg
     4621 gatgactgct agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat
     4681 ggcaggcata agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt
     4741 cggtgctggt ggtcttggag atatcattat ccgaggaacc aatgcaacag atggcacatc
     4801 gattatttta gcaggtgcat tgccaactgc attaatggtg attgtcacgg actggctttt
     4861 aggtattttg gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa
     4921 aaaacaaaag cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt
     4981 ttaaggcaat aatccactct gattcttccc tccttatggg caaagagttg cttttcttag
     5041 ctatttatcc attccgtata ctccaacttc atactgccaa cgggttcatc tctgcatcca
     5101 ccgactaagt ttttttctta tccgctaagg cattttgcca atccattttc ccatcactcg
     5161 tcatggaatt cataccacat gatctctaaa tactcgtctg cttgttcttt gaattgggca
     5221 tcaattctta catcctttgg cgtggctttc atttaagcct tttccatcag tcctcgattg
     5281 ttcgatgcct tcagctacat catcaaaact ggtgactgcc gtatcggtaa cttcgatcat
     5341 cttttataca atcgggaact tgccatcctc atccttggct aaaaatatat ctagccattc
     5401 cagcttctcg ccatgggagg tttgcggttc ctaaaaccgg tacatgattc ttatggactg
     5461 catccgtcgc aactagactt ggcggtacga tgatccttca ccagaagagc acccccgtaa
     5521 accaattgat cgatatattg ccaataagaa aagacattgg cgtaaaagat atcgatccct
     5581 cgtgatgcat tgccgttgat actgctattc atggccgaaa gcgccacgac tttgacttct
     5641 tccgtttgag tgacattcat gttcggcaat gtcgctttgg atacccgctc tgctaaagaa
     5701 acgacactgg tattgtattt cgtatctgta tcttttccaa aagaccatgc caacgaatcc
     5761 tctgggaacc aataagacga ctccggttga ttgtccattg cttttttcaa catacttttg
     5821 gctgtttctc gataaacgac atttccaatt gtcttcgcct cactttggcg ataatcgatg
     5881 gtcgttgcca ctgccaacac taacaacgat gctccaattt tactgatttt ccttgtcaca
     5941 cttcttcctc cttgttttac ataaacccgt caacattcca ttcctaaact caaacaatga
     6001 cccttcaaag gtggagatat aggaacaaat taaagaaatc atgcaataaa tccaagaagg
     6061 ttggttttag aacaaaaaaa gagtctcctt tttggggaga ctctttgtta actatctaaa
     6121 aaggttggct cttctcactt cactcataat gtgtccaggc agaataaatt tccacaactt
     6181 tattttcctc atcaactttg taaaccactc gatgctgtct attcagcctg cgtgagtatc
     6241 tgccttcatg tgtaggtctc agcttctcaa aagactgcgt gggcatatat ggatcttcct
     6301 tcaatgtctg aatcacttct tcaaattgtt tctttaaatt cgattgcttg atttttctta
     6361 aatccactgt cgcagagttt ttgattgcga ctgtatagtt actcatcaaa gagcttccca
     6421 atcaatatca tcgatattcg taaagccact gtcgtctttt tcgcggtctc gtactttatc
     6481 taaagtacct gtttgctcaa gaaaaagggt ttcttgaatc gctctccagt cttctagccc
     6541 aatcaataca gcattgtttc cactacgatc actaataatc tcgatttcag tatgattttc
     6601 atttacctct ttcaataact gataaaaatt tttacgagct gtgctaggat tcatcgttgt
     6661 attagccatt gtctctcctc ctttttctga tataattata acgtacttga ttaagtacgt
     6721 caaaaaatac aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa
     6781 aagacccctt tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc
     6841 ggtagccaaa cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa
     6901 agcctgtgag tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg
     6961 attgatatct tgaataacct gaggataccc acgaaagatt tgtaacggtt attcaaaact
     7021 gattttggcc gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgcca
     7081 ttcatgttca ctatcgattt gatacttgct tagaccgtcg ggtttgtgaa tcaccaatta
     7141 aaaatggcac tgaattatgg taacaaaaaa tggtcacttg acgaggctga gatttctact
     7201 tatgaagaca catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc
     7261 tgtacattat ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt
     7321 tacagctaaa taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat
     7381 agagggcacc tttagctggg aataagacaa aacagaccct ctagcttcaa ttaagagcta
     7441 gagggtctgt ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct
     7501 gacaacgata ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg
     7561 gagaatattc tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata
     7621 gttttgtttc gctgcatttt tacttacgat atctacataa cggataattg gattttcaat
     7681 cgagcgataa gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc
     7741 tgcttcgatt tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac
     7801 ataatcaccg atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc
     7861 gactaataca agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg
     7921 aagctgttct gctacttttt tataatgatc atggatctta taaaaaatga acataacgat
     7981 tggcataatg atgaagaaag gccaaatgtc ccctaaccga tagacgaaca aaatcgcaat
     8041 gattgcatat gagatgaacg cacctgtaat attggcaatc gacttgctta accatctttt
     8101 tccttctttt ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac
     8161 gccgatagag tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac
     8221 gatcgaacct agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc
     8281 ttgatacatg tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc
     8341 agaaaaaccg gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta
     8401 atacaaaata ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt
     8461 ttcaggaacg atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa
     8521 gatcaatgcc atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa
     8581 tggtacagca ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc
     8641 tcgtaaaatc aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg
     8701 caatggcaca gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac
     8761 aaggataaat gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag
     8821 attcagcatc atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag
     8881 tgctggcacc gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa
     8941 catgtagtcg atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc
     9001 actgctgacg acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga
     9061 tagcgttaat gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat
     9121 ggctgcagca gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga
     9181 tgataacgca tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc
     9241 attttcagca gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta
     9301 aactccttta cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga
     9361 tgttatagta cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa
     9421 aacagaaaaa tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt
     9481 ttatgggctt tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag
     9541 ttctatcctt tttgatatct taacttttga aagagagatg taaggttgct ttctacccat
     9601 cattcttact actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa
     9661 agcttgcatc tgcggtaata agcccaacca aattaaaaac ataagaagct gtttttattt
     9721 ggtcgttctt attacttgat gcagaacagc cttttcacaa cctcttggta cacggtgttc
     9781 aatcagctga ccctcggcat tagctcaaaa aacggcaaaa tatgaggaac tatttttcca
     9841 tttttttctc taatactcag gtcaaaacgc tgatttcaca acctcttttt ttaatgaaaa
     9901 atatggctct catgcgccgc ttctttgaat ttcgtcattt tttcttcgat gaacggatag
     9961 attgccgttc ctacgattcc aaagacaaca aacagaccga tcatgatcca gattgctttc
    10021 gtagcatttg gccagtaaat gcctcctgca gattcccgta acaagtttac cgcatgagta
    10081 aatggcaaga acggattgat catttggaag aatttccctg agacttggat cggatagttc
    10141 ccgcctccgc cagagatcga cagtactaag atgataatag cgatcccttt accgacatta
    10201 tcgaataatg cagccaagac atagacgatc atcatgaacg ctaaggcgat caagacagca
    10261 aatgacacgc tgtagaccgg attttggaca tctactccta gtaagaaaat attccctaat
    10321 gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg tcagcatacg agcaacgaat
    10381 tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta aatagtattc tgtcgtagca
    10441 acacttgaaa gaagaacagc gccgacccac agacatagtg cagtataaaa cggggtgcta
    10501 gctgaaccgt tatttgcgat tggataaagc gcattggttt gtagctcaac aggttgtgta
    10561 aagaagtcac tttcttttgt tgcatctagt ttcaataatt tgatgacttc gcctagatcg
    10621 acttcttgtt cgccttttcg gatcgcgtct gctgcttttt ggataccagc tttgatattt
    10681 ggccagtcat tgttgatcaa gtccgcagca gcgttcacag ctgtttcgac ttctggcatt
    10741 ttttcattta ctgttgtcaa tgtaccggtg atttcatttt ttagtgctgg ccaatcattt
    10801 tggataaaat cagcggccaa tcctaatttt tgctgaagga tcggtaattc attgttgtat
    10861 aggtctgccc cttcattgat gccattgacg atggtatcca tatttccatt cagcattgta
    10921 ttggcatcat ggatttcttg tccaattgca ggcatttctt tttgatattt ttctaaaatt
    10981 gccactgcat tcgttacagt tgctttcgtt gaactgagaa gcgaatcaaa gtcgatctgg
    11041 tccgctttgc ttagcgcccc ttgcgcagtc ccaatcgtat cgatgacttg gtctaataga
    11101 gaagtgacag tggaactgat ttgacctgta ttgataccag caatgatgcc attgatatta
    11161 ttaacaacgg catcaatttg atctagcaat tcatcaatag ccttcgtgtc ttcattttga
    11221 acagctgtgt tcaaagcaga taggcggttt tgcagattgc ttagcaaggg ttttaattga
    11281 tttagctgat caataattgg ctgcaatttc gtatttccag cacttgtttg caatttagtc
    11341 aggaactcga ccagtttatc gattgcattt tgttgttgag atattccgtc aataaagccg
    11401 ttcaatacat tgctgatgtt tttcttttca tcatcagtca gcttaccatc ttcaatagct
    11461 gttcttacag tatctattaa cgaacctgtc gttgttgcca cttgtccaat tgatttcaat
    11521 gtaacatcta cactgtttgt gatgcttggt atcgcatctt tcagctgaga ggcacctgat
    11581 tttgtttggt ttgctagatc acttgcctga ttttctaaat ttttcacatc tggcaagata
    11641 tcctgtactt gttggatgat cgtcaagcct tgttttgctt catcgattcc ttggttcatt
    11701 gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta gttgtttacc ggcattttgg
    11761 atctctggga ttttttcttg caaatcaagg atgatttttg cttgatcttt caactcaggc
    11821 attttgtcat tcaatgcaac gactttttca cccatctcat ctactttagg gatataatcc
    11881 acgaattcat ttgctttatt cagtttttcc ttgatttcag gtagttgact ttgtaattcg
    11941 aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt catctgttga taaaatcata
    12001 tctttgactt tattgatact tactaggttc gtatcgatgt cgtaacctat ctcattgaat
    12061 actttcaata aagtcgaact ggctgttttg ataaattcat ttgtgatttg cgattggatc
    12121 gaacttgctc ctttatcggt gattttcggt gcaatcgcat tgattttttc atttaccgta
    12181 tattcgattt ttggtttctt gatatcacca ctagtaaaac tcagcagatc ttctgagaaa
    12241 tcctttggca agtaaatacc tgcgtaatac tttccggaac ggactccgtc ttctaattgt
    12301 tcttttgaat caacgaattg ccatcctaat tgtttatttt tatgaagcga ttcgatgact
    12361 tgctccccaa tggccacttc tttaccttga aactctgctg gcttgtctgc actgtaaaca
    12421 gcaattggca gctctccagt atttccatat ggatcccata atgctttgat gttaaaccac
    12481 gcatatagcg aagggatgat catgatcgcg atgatcagaa gtgttgcaat cgggtttttg
    12541 aaaatccgtt tccagtcaag caaatataat cgaagcgtat ttttgattga ctgcatagtt
    12601 ctcccccttc ttcttttttc agctgaatta ctttaccatg tatttcatac ttttttcaat
    12661 cggctttttt caaaaaagtc gaatttttgg acatatcacg gcaattgtct cattgtaatc
    12721 tcttacatat tgagaaataa ttaaggatta cagactttac tttatcatga aataaaaggg
    12781 aaagataatt ttatgccaaa gtttttat
//
LOCUS       DAFBQH010000080        12598 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.082, whole genome shotgun sequence.
ACCESSION   DAFBQH010000080 DAFBQH010000000
VERSION     DAFBQH010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.082"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(11..160)
                     /locus_tag="LK694_002321"
     CDS             complement(11..160)
                     /locus_tag="LK694_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053155.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            556..756
                     /locus_tag="LK694_002322"
     CDS             556..756
                     /locus_tag="LK694_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK5053156.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            1324..1887
                     /locus_tag="LK694_002323"
     CDS             1324..1887
                     /locus_tag="LK694_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053157.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1948..2292)
                     /locus_tag="LK694_002324"
     CDS             complement(1948..2292)
                     /locus_tag="LK694_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053158.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(2389..2634)
                     /locus_tag="LK694_002325"
     CDS             complement(2389..2634)
                     /locus_tag="LK694_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5053159.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(2704..2958)
                     /locus_tag="LK694_002326"
     CDS             complement(2704..2958)
                     /locus_tag="LK694_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053160.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(3122..3445)
                     /locus_tag="LK694_002327"
     CDS             complement(3122..3445)
                     /locus_tag="LK694_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053161.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            3632..3705
                     /locus_tag="LK694_002328"
     tRNA            3632..3705
                     /locus_tag="LK694_002328"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3666..3668,aa:Ile,seq:cat)
     gene            complement(4090..4587)
                     /locus_tag="LK694_002329"
     CDS             complement(4090..4587)
                     /locus_tag="LK694_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK5053162.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(4627..5436)
                     /locus_tag="LK694_002330"
     CDS             complement(4627..5436)
                     /locus_tag="LK694_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK5053163.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            5770..6627
                     /locus_tag="LK694_002331"
     CDS             5770..6627
                     /locus_tag="LK694_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBK5053164.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            6620..7522
                     /locus_tag="LK694_002332"
     CDS             6620..7522
                     /locus_tag="LK694_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBK5053165.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            7555..7905
                     /locus_tag="LK694_002333"
     CDS             7555..7905
                     /locus_tag="LK694_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBK5053166.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7954..8877
                     /locus_tag="LK694_002334"
     CDS             7954..8877
                     /locus_tag="LK694_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBK5053167.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8955..9848
                     /locus_tag="LK694_002335"
     CDS             8955..9848
                     /locus_tag="LK694_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK5053168.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9886..10449
                     /gene="amaP"
                     /locus_tag="LK694_002336"
     CDS             9886..10449
                     /gene="amaP"
                     /locus_tag="LK694_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBK5053169.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            10463..10651
                     /locus_tag="LK694_002337"
     CDS             10463..10651
                     /locus_tag="LK694_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBK5053170.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            10664..11566
                     /locus_tag="LK694_002338"
     CDS             10664..11566
                     /locus_tag="LK694_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBK5053171.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            11598..11840
                     /locus_tag="LK694_002339"
     CDS             11598..11840
                     /locus_tag="LK694_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK5053172.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(12096..12467)
                     /locus_tag="LK694_002340"
     CDS             complement(12096..12467)
                     /locus_tag="LK694_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053173.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4461 a         2113 c         2366 g         3658 t
ORIGIN      
        1 tttccactcc ttatgcagat ttttttaatt cttgcactgc ttgttcgatc gtaacacctg
       61 tcgctacatg attggtattt tctttgtcaa aaacgacaaa tagattcaaa tctgtatcca
      121 aggttactcg atattgtata tctttgttat agaatgtcat agccattctc ctttctttaa
      181 atacttgctg aaaggcaacg aacgcgttga taacgaaatt ccatttataa gtataccacc
      241 aattgaaagg ttttcatagt taagaaattt taaagtcaaa agttgcagat aaaagcaaat
      301 aaacaagaaa acaaaaagtg aatcaatcgc ggttattcaa ctttttcttc gattcgaaga
      361 caatagaaga caatgaattt tatatttcat tgcattatcc ttgtgttatt agaaaaagca
      421 tgatatatta aatgagtaat ctattttgaa acgtaatttg agcgatatat ctacatagat
      481 aaaactcctt ttgcctatca aatatttatt gcaacgaaaa aacgtacatt gatacgtatc
      541 aggaggaatt tatttatgaa taacggtaca gtaaaatggt tcaacgcaga caaaggtttt
      601 ggctttatca ctggtgaaga tggaaatgat gtatttgcac atttttcagc tatccaaggt
      661 gacggcttca aaactttaga agaaggccaa gcagtgtctt acgatgttga agatggtcaa
      721 cgtggccctc aagcaacaaa cattgttaaa gcttaataaa aattgacttt taaccgtctt
      781 ttcgaaagaa aagacggttt ttttgtacga aaaaattcat tggtcgttga atgggctgaa
      841 tagtagtgta taaaacaatt ttgtggagcg tgaagctttt atggtagagg aaaatacgaa
      901 taaagtgatg ttgataaaaa gattcctatc ttgaatatga atcgagatgt cttgaaaatg
      961 aatgagtttg accaatcatt atcctatcga aatttacagt ttcgttcgat tggtctgtca
     1021 attaatgaaa aagtaaaaaa gaatatgttc taggaagagg gcggcacatc taaattttta
     1081 agtaacggac agttcaaata tcaagcattg aaagaggcta tcctactgtt tgaaggaata
     1141 cgaatatata aaaacgacaa agaggaaaaa gtagttcatt aaatcccttc aagtgatgaa
     1201 agaatgagat ctaataaaac ctgatctttc atctgttttt ttaataacgt aataattttt
     1261 ttattttttg tgctttcgcc gttattgtag agtgatttag cattcttatt tttaaaaata
     1321 actgtgttat atattaaaaa aggcataata gttataagct tttttaaaga atatttagaa
     1381 attattaatt ttcgtctgaa aacaaaaaaa gaaagcgtta ttattttagt ctattttcag
     1441 ggggggaacc ttatgataaa aaaactattt ttactatcaa tattaacgct agcaatggca
     1501 ggttgttctg actctggctt agcaactgaa ctgatcacaa aatcaactga agccacgaca
     1561 gaggagttaa aatgtggaaa cgatcacagt gactacagag gcttaaatcg acgatgcatg
     1621 ggaatattag gcagaagcaa ttgtaagcat cgatgtagta atcgaagaag gtgtagaaga
     1681 agcaacagaa aaatcaaaag aaagatcaaa agaagcaatc tgtgctgtgc atcgtataca
     1741 gacgtcgaag atgcggtact tttaaaccac gtcaggagat gttcatttat gtctagggaa
     1801 gttggcgatg aaccaaaact agagtacgct tttagttttg cacgtgagga tgaagataac
     1861 cctaaaactt acgacatttt agcttgatct aagatggtta aagcttttta atgaagtaag
     1921 aggagataga aggcttttct agttatatta ggtaagataa attccttttt gctccaaatt
     1981 tgctaaacat tctgctagat aaacttcatc gtgttccgga taaatcggca cgttgcgctc
     2041 tacagtaggt aaatcaaaaa atgcttcgca tgatttgcca cgatacatag gatttttcca
     2101 ctctggtgct gggctgtaac cacgtttttc catttcatcc atgacaagct gatgatattg
     2161 gaataatcta taaggagaat agtgaaaaac ataatccaca gtagcatgtt tctttcccca
     2221 gccatttcct cttaatgcac agcattcccg atgttgtcct aaaagctgtt gacgaggaag
     2281 ctttgaaatc aatttttcat gccataatca catagaaatg tctcctttat atatagattg
     2341 ttaaccttag acgttaaatg aaaaaaccag caccaaggct ggaaaaaatc acttcagttt
     2401 gcttgctgtt tctgagcgac caattaggta atcaacggaa atatcgtaaa agtcagcgat
     2461 atagattaat tcaatgatgc ttggaatttc ttctccttct tcgtacttac gatatttgaa
     2521 taaaggaata cttaacgcgc tagaaacagc tacttgagat aaagatcggc cagttcttaa
     2581 gaaattcaga cgacaacgta aagtatccag taaaatagct tcttcgatac tcatttaatc
     2641 acctttcatt atagtagaat agttataatg actatagaaa gctacacatg caatcaatag
     2701 tactcaatgt tttttattta aacgtatcaa ataaagaatg acgatcaata aagacaaaac
     2761 cagtcctgat aattcttcta taaaaacact atcgggttca aagtagttgt aattaaaaac
     2821 tttccacata aagtagatag ccggaagagt caaaatagct aaataaaacc gttctttgtt
     2881 ccaattcata agataaacaa tacttgcgac cagtgcgacc gcaggcaccc aaaacaaaac
     2941 tctcaccaac gtaatcatgt aacggactcc tcaacgattc atataataaa agataacatg
     3001 ttattgcata catcacaaat gaaatgctag gaaaataaaa gaagaataga tcaatgaaaa
     3061 atgaaaaagc agaacagttt tctgctgata agcaggaact gttccgctga atgtgtctta
     3121 tctatcgaga aaaaatcaat ggattgatat ctaagtaatt caatacatat aaaaagagga
     3181 cgatcgcatt cactaaaaag gaaagaatga tgatcccata gcgaatggaa acatatttaa
     3241 caaatacttt tgtttgaata cgggcatatc tttttccttt atagctgagc atcatgacaa
     3301 gaagcaaaag ggtaatcaca ccttgaaaaa tcattccaat aaacagaatt cctgaggttt
     3361 gataatttaa ttggagaaaa taagctgctg ccgcaactaa atcgaataaa atcgcaataa
     3421 atggcaaaat aatcactcct aacattgaga ataaataaaa gaataacatt ttttctaaaa
     3481 taagcaaata aaaattaaag taacaaaata gttatgacac aagtaaaaga aataagaata
     3541 acaatattat tttacttata ttttgaaact tgtgagagtt tgtcattcgt gctatagttg
     3601 aaaagtaaca aaaataaatt ttatttatga tgtaccttta gctcagttgg ttagagcaga
     3661 cggctcataa ccgtccggtc gtaggttcga gtcctacaag gtacataaga ggttgtgaaa
     3721 aagctgccct gcatcaagta ataagaacag ccaagtaaaa acagcttctt atgtttttac
     3781 ttggctgttc ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg
     3841 aaatcagcgt tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt
     3901 gccgtttgtt aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg
     3961 tgagcctgcc gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac
     4021 gtaggactaa aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga
     4081 tagatatttt tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga
     4141 ttctccgtgt tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa
     4201 agcactctgc aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac
     4261 ttcgatctct ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt
     4321 ttcaggtgga atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag
     4381 aagagggatc aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat
     4441 atcccgtgtg aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat
     4501 acgttctgcc aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg
     4561 attttgcata tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta
     4621 ttctttttaa tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt
     4681 tacttggttt gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc
     4741 gctgtaattc aacatatcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga
     4801 ttcgattcca aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg
     4861 cgtgatttct aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc
     4921 tctttgtagc ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct
     4981 aagagaagga tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa
     5041 atgttcataa taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc
     5101 ttgggtttct cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt
     5161 tctcagtgta tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt
     5221 catttcagca atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga
     5281 aaaaatatct ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt
     5341 ttcatcaaat ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag
     5401 caaggtaccg tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac
     5461 atatctattt tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg
     5521 ctttgtcatt atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta
     5581 gtttttggtc aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga
     5641 tgcgttttgt gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt
     5701 taaaaccaaa tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga
     5761 gtgattttga tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat
     5821 tatgctcctt acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga
     5881 cgcataaaat tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa
     5941 gaaacggtaa ataaatattc aagtaatgca agtatccaat tcctgacagt ggaaaaagat
     6001 atctacgaag acttcctagt cagtgaccat atcacaacta ccgcttactt aagaatttct
     6061 ttaccaaact tattagctaa agaggattat aagaaagtcc tttacttaga ttcagatgtg
     6121 ttagtactag acgatatcgt aaaactatat gatgagccgt taaatggaaa aacaatcggc
     6181 gctattatcg atccaggcca agtgaaagct ttggaaagat taggaatcga ttccgatgac
     6241 ttgtacttca attcaggagt gatggtgatt gacattgatc aatggaataa aaaagaaatt
     6301 acagaaaaaa ctatccatta cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa
     6361 gatgctttga atgctgtact ttatgaagat tgggaacaac ttcatccaaa atggaacatg
     6421 cagacttcgc tgatttttga acgtcatcca gcaccaaatg aaaaatatga acgtcagtat
     6481 aaagaaggaa atgaaaaacc ttcgatcgtt cactttacag gacatgataa accttggaat
     6541 acgttgaaag atcatccgta taccaattta tacttaaaaa aattagcaca cagtactcta
     6601 acgaaagtag gcgagatcaa tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac
     6661 aaaattcgta ccacatttag ctgcattatt tgtctcagta ttagataatt gcaacccgtc
     6721 taaatttgtt cggttctatg tgattgacga tgatatcgat tttgaaagta aacagctttt
     6781 acgcttctca gtgaaaaacg cacgaatgaa ctcagatgtg gaattcttga aaatcaataa
     6841 agaattcttt acaaatgtgg taatcagcga tcgtattcca gaaacagctt attatcgtat
     6901 tgcgattccg gaattgttcc gcggcacaga agttgaacgt atcttatata tggattgcga
     6961 catgatcgct ttacaagata tctctaaatt atggcgtctt gacttcggtg attcaatcgt
     7021 agcagctgtt gaagacgctg ggttccatca acgactggaa aaaatgaaaa tttcagctaa
     7081 atcaatgcgt tatttcaact caggtttgat gctgatcaat gtgaaaaaat ggttagatga
     7141 aaatattacg caaaaagtat tagatttcat cgaacataat ccagaaaaat tacgattcca
     7201 tgaccaagat gcattaaatg ctatcttaca tgaccgttgg ttaccacttc atccgcgttg
     7261 gaatgcacaa ggctatatca tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg
     7321 tgaatacgaa gaaactcgca acaatcctta cattatccat ttttcaggac atgtgaaacc
     7381 gtggagcaag gattttgaag ggccaacgaa gaaatattac gaaaaatatg cagggatgac
     7441 ggctttccgt tgtgtagcta aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa
     7501 aacagatgta gttgttaaat aatagaagac aacaactgaa gagggggagt aggaatggaa
     7561 attacaatgt ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa
     7621 atcgcactcg cgcttatcaa tgattccgat ccatttctaa gggacaaagg tgcttttgca
     7681 aaaggcagct tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa
     7741 atcgagcatc cattattgga catctataca tctaaattag agcaaaatta tttcggtaaa
     7801 cgattgaaca tggacttcaa caaacagctt gacagctttt cattagaaaa tgaaatcgca
     7861 gtactcgatt ataatatcaa attaaagaac tgcttcttca gctaatagtt tcgagaaagt
     7921 ccaaaaagga ggtatacatt gagatactac tttgtggatg aagaaaagaa tgtaatagaa
     7981 acagatgaaa aaaaatataa ttggctcgta cttgatagtg caaagcccga agagattgag
     8041 caagcaatcg aagaattcga gcttcctgat gatattttcg taggaaccga atatccggaa
     8101 gaagtttctc ggatggagcg cctttatgag acgaaactag agcatccatt ttctttggta
     8161 gtgattaatc tagatcagtc agataatcgt atcgaaaaaa aattaacgcc aatatctttt
     8221 gtactttccg ataatctctt gattacttgt atcgatagaa aaaccgaatt tgtggatcgg
     8281 ctgctgacaa agcatggaaa tcatttaaac tcgtttgaaa agatcatcac gtatacatta
     8341 ttagatattt acacccatta cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct
     8401 ttggataaag cagctcgtaa aacaacggaa aatgaagaac tgtataaaca ggcagatctt
     8461 gaaagagaca tcgtatatat cgatcatacc ttaagagatc aacgggaaac aatggatcaa
     8521 ttatggaatt ctgaaacttt catcaaacgt ttagacgatg aacagctact ctatgatgtc
     8581 catttgagac agcgacaaac agaaaaaatg atcgagatct atcgagattt attagagagt
     8641 attggtgatc tattcaacgg gatgatggac aataatctaa atcatatcat gaaatatcta
     8701 gattcagctg cattaatcat ttccgttccg gcgttgttcg cagggatttg gggaatgaat
     8761 acaggaggat taccgggtaa aagttctgct ctaggcttta tactcgtagt ctttggctct
     8821 ttgatagcag cagtagtttt aggttaccat ttataccgga aagattatac aaattaaaga
     8881 atctatttaa aacaggtatc aaacaaagaa atttgttttg gcaatacaat ttaaaaacta
     8941 ggaggaatac agcaatgaca gaaccaaaat taatcgaccc gcgaaacttg taccatacgg
     9001 agaaatttcc taaacaagat caggatacac cagctttaca gcagaatatg attcctgttc
     9061 ctgattgtgg agaagaatcc tatgaaggca ataatcaact agaaaaccgc cgtgtactaa
     9121 tcactggtgg agattcaggg atcggtcgtg cagcagcgat tgcatttgct agagaagggg
     9181 cagatatcgc tcttcaattc ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat
     9241 acgtgaaagt tgcaggacgt aagattgtct tactaccttt tgatcttcgt gacgaacaag
     9301 cacctaaaga aatcatagat aaagccgtga gtgaacttgg cgggctagat acgatggtac
     9361 taaacgcagg acagcaaatt tcttgtcagt ctattgctga gttaccactt gagcaagtca
     9421 aagacacatt tatgataaac atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc
     9481 atatcccagc aggaggagca atcgtaacaa caacttctgt tcaagcattc aatccaagcg
     9541 aacatttatt agattacgca gcaacaaaag cttcaatcgc taactttacg atcggtttag
     9601 caaaacagct tgcacctaaa ggaatccgtg taaatggtgt agcacctggt ccaatctgga
     9661 caccattaca actagacgaa ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa
     9721 gtttactaga acgtgcaggt cagccaggag aattagcacc agtttatgtt ttcttagcat
     9781 cgaataaagc aagttatgtt acagcccaaa tttacggagt aactggtgga gaagcgatca
     9841 acttataaga agttgatcgt ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta
     9901 aaaacgatcg gagtgatcgg aacattactt ttgctatctg tgcttttgtt tgcgatacta
     9961 ttaagtgcaa cctatccatt gccttttcag ctagagaagt ttcgattctt ttccatcact
    10021 aattcatacg tgcaacaata tgtattttgg cttgccgttg catttgctat tttgacaatt
    10081 atcacgttac ttgttttcct gttttatcca aaaacaatcg gaaactttct actgaaacat
    10141 ggtgatgggg agttgacgtt ggataaaaaa gcaattgaag gaatggttcg ttctcacttg
    10201 catgaagatg aattcatcca ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc
    10261 gccgtcaatg taaaagggga gttgaaacga acttcagcac tcattggtaa aacaggaaca
    10321 ttgatgaaag aaatcgaaaa tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa
    10381 gtatcggtga aatacacagg ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga
    10441 gtagaatagg gggggaccaa aaatgaaaga actattttcg acatacaagc tccctatcat
    10501 ctttggggta cttggattga tattagccgt tttatggatc accgtgggct ttttcaagac
    10561 cttattgatc ttgatcatgg cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac
    10621 gaaaatctta gatcactatt ttaaatctta ggaggaaaca tccatgaata acaataacaa
    10681 aaataataac aataaaatga acaaccaaaa accagtgtct gcacatccag acccaacgac
    10741 accaaaccaa tcaaatggtg tgaacgatga aaagaacaaa acagcaccag tatctgcaca
    10801 tccagatcca gtagtcccta acgttcaaca aggagtagaa gagaagaaac atgatgcaga
    10861 aaagcatgca cctcatcatc caggaactcc agaatctaaa gttccagaac aacatgatgc
    10921 aatcgatcct tctaaacctg tggaagagca aaaacatcct tctgaccatg cgaaagcaca
    10981 agaaaaactt agttctgacc acacaaacgg acaaggaaaa cttggttctg atcatacaaa
    11041 taaccaagga aagcatgatt ctgaacagga agattcaatg cgcggtgagc tgaattatga
    11101 agataaagtt gtgcaaaaaa tcatcggaat cgcaattgaa caagtagacg gtttattatc
    11161 tgcaaacggt ggtttcttct ctaatgtggc aggaaaactt gtgaatacag ataatgtaac
    11221 ggctgggatc gaaacagaag tcggtaaaaa acaagtagca gttgatatgg atgtcattgt
    11281 ggaatatggc aaagatatcg aaaaaatctt tgaagaaatg caagaagtaa tcggcaaaga
    11341 agtaaaaaat atgacacatt tagaagtgat cgaagtcaac gctaacgtag tggacatcaa
    11401 aacaaaagaa gaatttgaaa aagatcaaga aacggtgcaa gacaaagtaa ctgacgcagc
    11461 gaaaaaaacc ggtgaatttg cttcgaatca aacagataaa gtaaaatcag cagctggaag
    11521 cggcgcacaa aaagtaaaag aaaatacaga accacgggtt cagtaataag gaagtaaaac
    11581 attaggagga acgaaacatg ggatttattt ggtcacttat cgtcggagga attatcggag
    11641 caattgcagg agcaatcacg aataaaggtt cttcgatggg gattattgca aatgtcgtag
    11701 caggtttagt tggatcagca cttggtcaag cattattagg ttcttgggga ccaagtattg
    11761 caggaatggc aatcgtgcca tcattgattg gtgccatcat tttagtacta atcgtatcat
    11821 tctttgctcg aaaactgtaa aaagactttt aaaaagaggt cgtgaaaaag ctgttcttca
    11881 tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt ggttgggcat
    11941 attatcgaag atgcaagctt ttgaacactg tttatacgga ataagaggct gtgacaagac
    12001 ttttgtcaca gcctcttatt tatgcgggca aattcccaat tagattttta aaagtttttc
    12061 cttaaatgtg tctttattga tacagagaag cttctttaat aaaacaatgc ctccctaaat
    12121 tttcttgaga taggaaaggt gaacatacaa aataatacga ccactttgaa aacccagatg
    12181 atccacaaat ggatcgtatg atactgatga agtaaaaagc aatggatcag aaactcaaag
    12241 agattgaaaa agacaaggaa gaaataatat ctctttttta ctcttgtctg ttttagccaa
    12301 taatagctcg taaagtaatc tagacaaaca aatagaagta aacaaagttt cgtaagagca
    12361 gcaatcgtat catgttgctc tgctagacga ttgacgagca ggaaaaatgt tggtaatgga
    12421 atatagatca cgagtaatga aagaaaaaag taaacaataa atggcatact attcctccta
    12481 attgtgtgag ttcgccctca ttttaaaata acgatatttg tttgtgaagc tttttcgtgt
    12541 cgtaacaaat gttatctaat gtatttttaa ataaataatc attattatca aaataatg
//
LOCUS       DAFBQH010000081        12583 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.083, whole genome shotgun sequence.
ACCESSION   DAFBQH010000081 DAFBQH010000000
VERSION     DAFBQH010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.083"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            5..2377
                     /locus_tag="LK694_002341"
     CDS             5..2377
                     /locus_tag="LK694_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053174.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3907
                     /locus_tag="LK694_002342"
     CDS             2438..3907
                     /locus_tag="LK694_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBK5053175.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3918..5927
                     /gene="topB"
                     /locus_tag="LK694_002343"
     CDS             3918..5927
                     /gene="topB"
                     /locus_tag="LK694_002343"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBK5053176.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            6043..6192
                     /locus_tag="LK694_002344"
     CDS             6043..6192
                     /locus_tag="LK694_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053177.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6273..6869
                     /locus_tag="LK694_002345"
     CDS             6273..6869
                     /locus_tag="LK694_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBK5053178.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPEKTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            6882..7781
                     /locus_tag="LK694_002346"
     CDS             6882..7781
                     /locus_tag="LK694_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBK5053179.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            7784..7915
                     /gene="ssb"
                     /locus_tag="LK694_002347"
     CDS             7784..7915
                     /gene="ssb"
                     /locus_tag="LK694_002347"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK5053180.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            7937..8269
                     /gene="ssb"
                     /locus_tag="LK694_002348"
     CDS             7937..8269
                     /gene="ssb"
                     /locus_tag="LK694_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK5053181.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            8314..8547
                     /locus_tag="LK694_002349"
     CDS             8314..8547
                     /locus_tag="LK694_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053182.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(8706..8828)
                     /locus_tag="LK694_002350"
     CDS             complement(8706..8828)
                     /locus_tag="LK694_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HBK5053183.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            9685..9891
                     /locus_tag="LK694_002351"
     CDS             9685..9891
                     /locus_tag="LK694_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBK5053184.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9891..10142
                     /locus_tag="LK694_002352"
     CDS             9891..10142
                     /locus_tag="LK694_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053185.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            10158..10313
                     /locus_tag="LK694_002353"
     CDS             10158..10313
                     /locus_tag="LK694_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053186.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIE"
     gene            10323..10595
                     /locus_tag="LK694_002354"
     CDS             10323..10595
                     /locus_tag="LK694_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053187.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            11040..11219
                     /locus_tag="LK694_002355"
     CDS             11040..11219
                     /locus_tag="LK694_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053188.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            11278..11634
                     /locus_tag="LK694_002356"
     CDS             11278..11634
                     /locus_tag="LK694_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053189.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         4637 a         2068 c         2624 g         3254 t
ORIGIN      
        1 ggacatggca gaagaaaaag aaccactaga caatagtcgc ttgggaaaat ccaaaagaaa
       61 attagtacga ttgcaaaatg aattaaacga acaaattgag aagatgtttg aacaccagag
      121 aaagaccaac ggtcagccaa tgaacgataa gagaaatggg cattcttggt ttaggcaaca
      181 agaacgttta gaaaacaaag tgcatagtct gagagaagag atcaagcaac aagaaaaaca
      241 ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 cctggatatg acaatcgaaa atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg actttttcag ccgctacgat ccgaaaatac caacggaaat tggaaacatt
      421 agaacagctg aaagaaagat cagaaaaagg aaaagaaaat cttcttcctg aagtacaagc
      481 aattattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 agactatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctgc
      661 gccaactgat tctttgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tagaattgat
      781 cgatcaactc aaaacgttgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaagaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccca tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 aaaggaacaa aaacaaacgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaagcaaga
     1081 agtcaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtgaaa gaacttttga attttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ctgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actacacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgggg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cttcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 ggcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctgtagaaac ggcagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtga ttttaaaagg
     2221 aaaaagttat ggaaaagaga ttcaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg aaacagtgtc agaagtggcc aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg cgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg tttgaaaatg accgaaaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagact atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ctctaagcaa caatttaaag cagccacaga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtagaa aatcttcatt ttgagacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgctgaacta aagccgaaac atgaacggca aaatttttta gttggagacg accgtttaga
     2941 tcatgcggtt gcttttttat ggaaagatcc acagacagga gagacagtag gggcaagtta
     3001 tcaaggaacg atcgtcgatt ttaatcgttt cggtaaacgg ggaacgtata aacacattga
     3061 caagaatcct acgccaaatc acggatttaa tttaaaaata ggcgatccga agcatttaaa
     3121 attctttgaa agtagtattg atctgttaag ttacgctgca ttgaatcggg agaaactaca
     3181 agatgcctgg ttagtttcga tggacgggtt aaaacatcat gtaatcagtc attacgttga
     3241 agagtcgatt tctgaattaa gacgaaaaca aacatttcct caatcgattg aaatatgtgt
     3301 ggacaatgat cgtgcaggac atatttttta tgaaaaagaa cagatgaagg gaatcgttga
     3361 tccttttacc aataagaaaa tccgttgtga gcgaggaatt ccaaatgatt ggcaagtacc
     3421 taaagaatac aaagctactt atgaagcagt cgcaaaagaa atgagtgttg aacctgaagc
     3481 aattatggcc atacacaaaa ctgaaactaa tttacagctg acaaatcaat tggtgtctgc
     3541 tcatgatgtt caatctacgt ttggaaagat gttggcgaag ggcgaaccag tagaaacaat
     3601 tgatttgaaa gaagcatgta ccacggtcgc gaaggaactg aaagtatgtg aacgcgcaga
     3661 tggaacatac aactttgatc gtttttatag tagaaaagcc aacataaaag acgttaatgc
     3721 aggaatcctt ctttcctata aagcagaaca atactacaaa ggctataaaa agcatgaaca
     3781 tgaatttgta cctgaagtga aaaaggattg gaatgatcaa ttgaagcatg agatccagca
     3841 acaagaaatc agaaagcaaa aacgtgcgat gttgtttcaa caaggcagac aacaagaaag
     3901 ggagtgaagt cgtcatgatg aaaaccgtca ttttagcaga aaaaccgtca caagcgaagg
     3961 cttatgccga tagcttttct aaagctaccc gaaaagatgg gtattttgag atccaagatc
     4021 gattgtttac aggagaaact gtgatcacgt atggttttgg tcatttagtc gagttagatt
     4081 cgcccgatat gtacgatgaa aaatggaaac aatgggcatt agaacatttg cctattttcc
     4141 ccaatcaata ccactatcat gttcctaaag ataagaaaaa gcagtttagt gtcgtcaaac
     4201 gacagctgca gtcagctgat acgattgtga tcgcgactga tagcgatcgt gaaggggagc
     4261 tgattgcctg gtcaatcatt caacaagcag gcgccaatca aggaaaaacc tttaaaagac
     4321 tttggatcaa ctccttagaa aaagaagcca tttatcaggg attccaacag ttacgagacg
     4381 cagaagagac ctatccaaaa tttgaagaag tacaagcacg tcagatcgcc gattggttaa
     4441 ttgggatgaa cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa
     4501 gtttttcttt aggtagagtc caaaccccaa cactctatat gatttatcaa ttgcaggaaa
     4561 agattagaaa cttccaaaaa gaaccatatt ttgaaggaaa agcccaagta atcgctcaaa
     4621 atggcgcatt tgatgcgaag cttgatccaa atgaaaccca agcgacacaa gaagcatttg
     4681 aagcctattt aaaagaaaaa ggtgttcaat taggaaaaca gccaggtacg atccttcaag
     4741 tcgaaacaga gaaaaaaagt gcggctagcc cacgtctttt ttctttatcg agccttcagt
     4801 ctaaaatgaa tcaactcatg aaagctagtg ccaaagatac tttggaagcc atgcaagggt
     4861 tgtatgaagg aaaatatcta agctatccgc gaacggatac cccttatatt acggaagggg
     4921 aatatgcgta tctgttggat cacttagacg aatacaagca ttttttaaaa gccgaagcga
     4981 ttccgactcc tattcataca ccgaatagtc ggtatgtgaa taataagaaa gtccaggaac
     5041 attacgcgat cattcctaca aaaacggtta tgacagcagc ggcttttgaa caactaagtc
     5101 cactgcagca ggctatttat gaacaagtgt taaaaacaac ggtggccatg tttgcggaaa
     5161 agtataccta tgaagaaaca acgatcttga cgcaagttca gcagcttcag ttaaaggcaa
     5221 tcgaaaaggt gccgctagat ctaggttgga agaagttatt tggaaaagag agcgacggaa
     5281 aagagaagga agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtgg
     5341 atctacaagt tcttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga
     5401 tcactgcaat gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga
     5461 aagaagtgga aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac
     5521 aaaaggaata tatcaaggta gagaaaaata agctcgtggt gacgaataaa gggattttgc
     5581 tttgccaagc cgtggaaaaa gagccgctgc ttacgagcgc cgaaatgacc gccaaatggg
     5641 aaagctactt gttaaagatc ggtgaacgaa aaggaacaca aaccactttt ctagccaata
     5701 tccaaaaatt tgtctctcac ttattggaag tcgtaccagg gcaaatccaa tccactgatt
     5761 ttggatcgac gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt
     5821 taggaatttg tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt
     5881 gtacgtctga agcctgtgat tttaaaaggt cagaagggtt catttgaagc aacgattgtt
     5941 ttaaaagaag acttcactac aggctttgaa ttttctgaaa agaaaaaaac gaacttcaag
     6001 aaaagaacac gccgcactgc aaaataatgg agggttaatc ttatgcgtaa taccacacac
     6061 aaacaacaag aaatcgaaaa tcaaaaagcc aaaattcgtt cagaacagga aaaaatgcgt
     6121 gccagcaaac gaagactgca gcaattgaag aaccagcaga ggattcaaaa agaaatcgaa
     6181 ttagatcgat aaaatcttcc taactattgt atacgtataa acttatgtta tactgttttt
     6241 ataagtttat acgtatacaa aggagggaga aaatgacaaa agcaatggta tacaagctgc
     6301 tagaagaatt taatggaact ttggctttga aggatgccaa aaaagcgggc atttctcctg
     6361 taacgatcaa acgtatggta gatcgagggg agctggatcg agagtatcca ggttttttca
     6421 ccttacctgg tcaatttcct gatgaattgt ttatggcgca aaaaaaatat gaacgcggca
     6481 ttatttcaca tattaccgcg ttagatttgt atgatttaac agatatgatc cctagacaga
     6541 tcgatctgac tgtaccttac ggttatcacg tttctgaaaa aggattgaag gaattcgcag
     6601 ttgagctaca ctatacaaaa gcggaatggt atgaattagg taagattgaa ataaaaagtc
     6661 gctatgggaa tcctattata gcttatgatc cagaaaaaac cttgtgtgac atttggaatc
     6721 cctggtataa cgttgaggat gaaatcaaag taaaagcaat caaaaactac atggagtcgg
     6781 acagaaaaaa tcttagaaaa ttgaatgagt atcgacgaat tttgcctaca gataaaacga
     6841 tgcgctctta tattatggct ttaaattaaa ggagaataca catgacacct acgcaattaa
     6901 aagcaaaagt gaagaatatt tctagagaaa aagaagttga tccgcagctt gttttaagac
     6961 attttatgat ggaaaaattc ttggaaaaaa tctcagagtc tccttatcgt gaaaattttg
     7021 tgttaaaagg cggcttctta attggctcaa agtatggcat tgaaaatcga acaactaaag
     7081 acattgatac gactttacgg gaaatgaaag taaccaaaga aacattgaca acggttctaa
     7141 acgatatttt ttcaacgccg acgaaagaag gcattcaatt tgagattcaa ggaatgaaag
     7201 aaacgaggga agctgattat tatccagggt ttagcttacg tgtactggca catttggaaa
     7261 acatgcggcc agattttaag gtagatgtta ctacaggtga ctcgatctat cctgcgacga
     7321 tcactcacag tcataaattg atgtttgagg atcgaacgat tcctttggaa tcctatccta
     7381 cagaacaaat tattgctgaa aaattaagtg caacctttga ttttttaaca gataattcga
     7441 gaggaaaaga tttttatgat ctctatacta ttcctaaaat ggagaagatc gatgagaaga
     7501 ccttgaaaga tagtgtgaga aatacattta cacgtcgagg aaaaactgat ccgttaaaga
     7561 tatactatga aaaggatatg gatgtactga aaaatagtca cgagctaaaa gatacctgga
     7621 agaaatatca aaaaagcaat ccttatgctg cgtcgatcac gtacgatgag acaatggatt
     7681 cgattagtaa tttgatggaa aaagtgattc acgtcgaaga aaaagagcga aaactagctt
     7741 atcaaagaca ccaacagatg aatagagaaa tggagagata aagatgatta ataatgtgac
     7801 attagtagga cgcttaacca aagatcctga tttacgttat accgcaagtg gtactgcagt
     7861 agcgactttt accctagccg tcaaccgaaa tttcacgaat caaaacggca attgagaagc
     7921 cgattttatt aactgcgtga tttggcgcaa accagcagaa acaatggcga cattagctaa
     7981 aaaaggtagc ttgattggtg tagttgggcg gattcaaacg cggacttacg acaaccagca
     8041 aggacaacgt gtatatgtga ctgaagttgt agcagataac tttcaactat tagaaagtaa
     8101 agcagcgaca gagagccgtg cgcacgctga tcaatcgtcc accagtccaa gcaccgccac
     8161 ctttgaacaa cggaatacag cgacgcctaa caacaatgga ttgaacgctt cacagaatcc
     8221 atttggtgga caatcaatcg atattagcga tgatgatcta ccgttttagt ttgagaaaga
     8281 aggagtaagc atggcaaaaa aattagtctg acgatggatc tattgatacc cgtaattgca
     8341 tttatagacg ggatttcttt gggcgtcact gaaaatcctt tggtaaatag aattgcgaaa
     8401 ggatcactat ttcttgtatt cgtgtatctt gtctgttctt atcttttaaa aagagcagga
     8461 aaggataatt ccttttggcg attgactaaa gtcacagtag cagcgacagc tgttatactt
     8521 acggctattc taccgcttta tatgtagagt taacgggaaa tcgttgaact tcttgcgaaa
     8581 tattgctata atgaacatga aaaagaggag ttgcgctaac aacccctcat taacagcacc
     8641 gattaaagaa cggtggcaga atattagtta tttggaaata accgctgacc tgctaaagta
     8701 gacggttatt tctttttgtc atttttaagc atatttacaa tcaaactaat caaagcaatg
     8761 gtaaacatac caaaaccaag aatggtttga attgtctcat atgcggacaa aaggcttact
     8821 cctttcatag attttagcac ttacattcat aagcaccacc acctttccag aagtcgccac
     8881 cgtcttcaaa ctttactgtc ttgactatta tagcatatct aaaatcgaat aaaaattctc
     8941 aattttagct gtttttgcta taattttagc gaggtgatcg tgatggaaca atatattggt
     9001 ttactcgata aattagggtg atcaagacac agccattgct tgtgcgattt acattacgca
     9061 cgagtcatga accaatcaat tgtgtagtgg ccaatataga aatagcggat aaattgctga
     9121 ttttggaaga aggcaagtat actatttcag tgacaggtca cttcaataaa cgaaagcaac
     9181 tagtcattga atcaatgcaa attagaaacc ctgatcattt tacgcgatcg atgagcatat
     9241 aaaagaagag ctgcagtcct aaatgaggat tgtagctctt tttgttttac atagaaacag
     9301 taaaaatgaa aaggcctgtt taggaaggca caagcgcctt ctcaattgaa agacctttct
     9361 ctcactccaa ccgccaccgc cgttcgagaa gaaaggaagt gaacgctacg cgttcactaa
     9421 gataaagggt cttgcatagc aagacaaaat agaaaaaatt tcctctcacc aaaaggttcg
     9481 ctcctgttcg ttatctcttt attgatttgt cagaccggca acaagttgcc ttttatcacc
     9541 aaaaagcgac aaggacaagg gtaaatggca cgctgcgcgc ccttgtcttg tcgcttttcg
     9601 ttgattcatt ctggccaaca aatcaataaa gaaagaacta acgaaaggaa gtaaaacctt
     9661 tcctaagagg aggcagaatc aatcatgcga aagaaatttg attattgggg tgttcccttc
     9721 agcgaattat tcccaaacta tcatgcacca catactgtcg aatgcgattg tggagaaaga
     9781 gcgaaatgta tcaaaagcta ttgtttgtat caatgtccaa catgcgggaa aaaatatacc
     9841 ttgtcttatg gcgattatat tttaattgat gagaaaggaa agaaacgctg atgaaaacac
     9901 aacacaaatt attgactgta aaagaagtcg ttcaaatttt ggtggacaaa gaagtcattt
     9961 ctgaaaaagt agcagaagtt ttacttattc cgcggtactg gacgatagaa acccctttag
    10021 tagaggatgc accgactatg ctatttgaag acctagtgaa gattgcagga tatgaactgc
    10081 attatcaaga aaacttggaa acttatgaaa acgaatattg gttaaccaaa ttagagaact
    10141 aaaaaggaga aaaaaatatg gctcaaaatt actatgacga atttgtcaaa ttacctttgg
    10201 ataaaatggc acaaaaaatg gaggatatga cgttcctata caacgaaaca cgtgttccaa
    10261 aaaaacatta taaagaaaaa ttgtcggtag ctgtggaaga aatgattgaa taaggtgtcg
    10321 aaatgaatct tattgcgacg tactatcgaa cactggaaga actgaaaaag caaaatgcaa
    10381 aatggttttt ccaagcctta ctatgcttgg aagtaggtgt caaaccaagc actattaaac
    10441 cttctgaata ccaagcttta gaactaacgt atgcgaaatt tatcgaaacg aaaaaagcca
    10501 agactgtttc aagtgaatgg ttagattatt ttgaaaatat caataagtat ggcgcatatt
    10561 acaccatgaa aaaggaagac aacgaaaatg aataaggtga tttatcaact aacaacggat
    10621 tccatatcaa cgatttgaaa gaaaaaaagg tcaataatca tttataccga aaagtgagaa
    10681 tgtgtgaaac aaggacaaaa aacgcctagt aactaaggaa atggttctta tttcgcacca
    10741 atcgctccaa ccgcccccgc cgcttaggtg gtgcgaaata aacgggcaaa gcccgataac
    10801 ttaagagaaa gaaggtgatc catttgaaca agattattcc cttattgtta gtactgtttg
    10861 ttggcgaaat acttatgcga gatagtgcag ctgtacagtt aaacctcgct gtatttttgg
    10921 taatactggt gttttgtttg atcttttggc ggtttgtatt agtcctcgtg agtctactat
    10981 tcaagatgat tgcagccata tttttcatag ggttatttcg atgaaaggag tgagtaccaa
    11041 tgccaattgc aataggaaat aaacggttac ccgtgacatt agatgaaaag agacaaaaag
    11101 aattgcagca actaaagcag aagtatggca aaagtgaatc taagattatg tgtattgcgt
    11161 tagatctatt gatcgcgcaa gaaaaagcag gatttgatgt accagcgcta aaaaagtgac
    11221 gtcacttttt aatttgatct atataagtga tatcactttt accaaggagg gaattaaatg
    11281 ttaaaaacta aaaagaattc gtacaaatta aagcttagta tactatctat tatttgtctt
    11341 ttaggcatga taattagtgg tcatttgtat agagtggcat atacgcagtt tattgagtac
    11401 aaggttcact ctttaggatt agctggggat cgatacgttg aatatgtcca ttattcatta
    11461 aaatgttctt catatattct tctttggctt attttttcat tgtttgaatt gctaaattta
    11521 atagcaatat cttcatatca cttagataag ctaaatacca aatctagccg ttataacgaa
    11581 gtgtttaagg aaaatgtgaa gttagaaagt gacttaaaca agctaaaaaa ataaaaagaa
    11641 gaaaacataa gttaactaaa attattttat taagaatgat ggaatataag gataaggtta
    11701 ccaacataac gatcattact tctctgatat gctatggact tctatcaatc agtattttgt
    11761 ctatagatta tggagtaact atactgcaag ccttagtttt ggctatgcct atgtatctat
    11821 ctttgtttat catgcatgaa atatataagc tatttgtttt atcaaaaaat gtagaaatac
    11881 gggtaaatat tttggtgaat agttatcaaa aagaactcaa agaaagaaga cggtggcggt
    11941 atttattgaa aggatgaaaa agtaggattt gacatactgg tattcagaaa gtgccgtcac
    12001 ttttaaaaca atggaaaagg tggagataag atgaagaaaa aacaaactaa actatcatta
    12061 gcagatattt aacacagtta accgcaactg aagatggtgt tgttgaattt gaacaatctg
    12121 aaattgcggt agtagatgat cgttacctta aaatgcctta cttctttgac caagcaaagg
    12181 tcatttgcct ttgtcactac gatggggtac gtgattttag gcgttaaaaa gtagtgatta
    12241 tatcaacttt acgagtaaat ataaatttag acatgaaact gaattatcca atggaaaatc
    12301 atggtttata atctagaaaa attatagtaa tgattggatt atatatattc tttcgtattt
    12361 tcgtacaact gtagcttgtc aaaattgaga aatttaaccg attaggtact ttacatcgtt
    12421 atttgggtaa tttctaaaac atgaagctta ccaacgaata aactgagact atcttattaa
    12481 ttgatcaata taagaaaata tctttgagga gatgatgtac tatgttttcg tctatccacg
    12541 attggttagg tggtcttttc ggcggtggag gtaaaggttt tta
//
LOCUS       DAFBQH010000082        11444 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.084, whole genome shotgun sequence.
ACCESSION   DAFBQH010000082 DAFBQH010000000
VERSION     DAFBQH010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.084"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            21..191
                     /locus_tag="LK694_002357"
     CDS             21..191
                     /locus_tag="LK694_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053190.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            406..1665
                     /gene="lysA"
                     /locus_tag="LK694_002358"
     CDS             406..1665
                     /gene="lysA"
                     /locus_tag="LK694_002358"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBK5053191.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1652..2452
                     /gene="dapF"
                     /locus_tag="LK694_002359"
     CDS             1652..2452
                     /gene="dapF"
                     /locus_tag="LK694_002359"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBK5053192.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2455..3336
                     /locus_tag="LK694_002360"
     CDS             2455..3336
                     /locus_tag="LK694_002360"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBK5053193.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3333..4049
                     /locus_tag="LK694_002361"
     CDS             3333..4049
                     /locus_tag="LK694_002361"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBK5053194.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            4055..4750
                     /gene="dapD"
                     /locus_tag="LK694_002362"
     CDS             4055..4750
                     /gene="dapD"
                     /locus_tag="LK694_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBK5053195.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4766..5767
                     /locus_tag="LK694_002363"
     CDS             4766..5767
                     /locus_tag="LK694_002363"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBK5053196.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5764..7125
                     /locus_tag="LK694_002364"
     CDS             5764..7125
                     /locus_tag="LK694_002364"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBK5053197.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            7085..8236
                     /locus_tag="LK694_002365"
     CDS             7085..8236
                     /locus_tag="LK694_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBK5053198.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            8211..9380
                     /locus_tag="LK694_002366"
     CDS             8211..9380
                     /locus_tag="LK694_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK5053199.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9555..10934
                     /locus_tag="LK694_002367"
     CDS             9555..10934
                     /locus_tag="LK694_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK5053200.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            11215..11444
                     /locus_tag="LK694_002368"
                     /pseudo
     CDS             11215..11444
                     /locus_tag="LK694_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3828 a         1972 c         2535 g         3109 t
ORIGIN      
        1 ttttttgtgg aatcagcaaa ttgaaaaata aaaaagtatt tgattttatt tatattttga
       61 aagatgaatg gcaaaagcta gagaaagaaa aaactgcact tatttatgaa gaaattgaaa
      121 tgattcataa aattgaaaat atccgaacta ttccgaacta tattgaagcg aaaatatttt
      181 ttcatcacta aatagtgttg aatttatgtt tttatgtata aatagcttct aatagttcgg
      241 gtattttgtt tttataacca tttttattct tatttttaaa aaaatatttc gaaatatact
      301 agacaattca gaaaattttg tttaatatta ttccaataaa attcaaatct ttatcaaatg
      361 aaattccaat aaaaacggaa aaaaagtgaa aagaggaatg aaaaaatgac agaattgata
      421 attggcggag tcacagcaag cacattggca gaaaagtata agacacctct gtatgtgtat
      481 gatgaagaaa agatgagaca aacgatgctg tcattcaagc gaggctttgt atcgaaacgt
      541 tttgatacaa aagtgcttta cgcctctaaa gcattccaaa cgattgagat gctgcattta
      601 gttcgtgaat atggattcgg gctagatgtt gtcagtggtg gggaaatcta taccgctttg
      661 caagctgatt ttcctagaga tactatctac ttccatggta acaacaagac gccagaggaa
      721 ctgatctatg cacttgaaaa tgatttgttg catattgttg ccgataattt gatggaagtt
      781 gaattattag cagaattaag ccaacgctat ttaaaaaaaa tttgtatcat gcttcggttg
      841 aatgtcggag tagaagcaca tactcatgaa tatatcgtca ctgcgcatat tgattcaaaa
      901 ttcggcatga gctatgaaag taaagagtgc caacaagccc ttcagttgat aaaagaaagc
      961 agctatctag agttagaagg atttcatgct catatcgggt cacaaatttt tgatgttact
     1021 gcttggttag ctgaaatcga caaattgacg aattacttaa ccgattttga ccaacctctt
     1081 tccttgaatc ttggcggagg gtttgggatt cgttatacac aaaatgatca gccgatccca
     1141 gtcgaagagg cactaaaaaa actgattgaa tatacggaag aagctttgga aaaacgaaaa
     1201 ctgaccattc gtcaattact gatcgaaccg ggtagaagta ttgttggaga agctggtaca
     1261 acactataca cgattggttt catcaaaaag acaccacata aaaaatatta ctttgtagat
     1321 ggcgggatga cagataatat ccgtccagct ctgtatcagg cagaatacga ttgtgattta
     1381 gcagcaaaac tgtcggaaga aaaaacagag aaagtgacga ttgcaggaaa aatgtgtgaa
     1441 tcaggcgatg tggtaattaa agaagcttat ttgccgaaag cagaatcggg tgatctttta
     1501 gctgtttatt ccacgggagc ttatggatac tcgatgagca gcaactacaa tcgagcgaca
     1561 cgaccagcag ttgtttttgt caataatggc caatcaagac tgattgttcg cagacaagca
     1621 ttttctgatc tactaagggg ggaagtttcc tatgatcctt cataaatata atggctgcgg
     1681 aaatgatttt atcttattag attacgcaga agacacagat tatagtcaat tagccgcttc
     1741 tttatgcaca aaagaaaaat atgatataga cggattgatc gccgtaaaaa tcgatccgtt
     1801 ggaaatgatc tattacaaca aagatggcag ccgcgcgccg atgtgcggaa atggcattcg
     1861 ctgttttgct cgatacgtaa atgatcagaa catgatgaat gaacaggaat tcaacgtaga
     1921 gacattagca ggcacaagaa aaattacggt gttggatccc tcaccttttt attgcagcgt
     1981 agatatggga gtgccagatg attctccgga aaaaatcaaa gctaatcaaa cttatccaat
     2041 aaaaaaccag gaaatagaga tcaatggtga aaaagtaacg atcactagtt tatttttagg
     2101 aacgattcat accgttgttt ttgttgaaaa tgcgcgtgag gaagtctgga gaaaaagagg
     2161 tgaactgatc tgtcatcatc ctttgttcaa agaaaaaacc aatgtcaact ttgtagaagt
     2221 agtcaatgaa caagaattga tcgtccgtac ctatgaaaga ggagtaggtt ggacacttgc
     2281 ttgcggtaca ggttgctgtg cctcttatgt ggttgctaag gaaactggga aaatttcagc
     2341 agatcaggta gttgttcact tggaacaagg agacttgatg atctctggtg aggatgcgat
     2401 acaaatggct ggcccagcag ttcatgaatg gacaagaata ttggaggaat gaagatgata
     2461 aaaggttcga tcgtggcgct gatcacgccg atgaatgaag agggatcagt agattatgct
     2521 ggtttggaaa aattgatcca attccattta gatgaacaaa cagatggatt attagtttta
     2581 ggtacaacag gagaatcttc tacgctcaca caatctgagg aggagcagat ccttcaactg
     2641 acagtaaaga aagtggctgg ccgtgtgccg gtgattgcag gagctggaac gaataatacg
     2701 aaagaaacga ttgaaaaagc gaagcatttt gcttctttgg gggcagacgc acttttggtc
     2761 atcacccctt actacaacaa aacaagtgat gcaggactag ctgctcattt tacagcaatt
     2821 gcagaagcct caccgattcc gcttattttg tataatgttc cttctagaac aggcatgtcg
     2881 attccaatcc atgtattagt aaatttagca gaacatccta atatcattgg tttgaaagaa
     2941 gcctcaggag atatggctta tgtgatggat gccgctcggt tgattggaga agaattcttc
     3001 ctttacagtg gaaatgatga tttgatccta ccagtaatga gtgtaggagg aagcggtgta
     3061 ataagtgtat gggcgaatat ccaacctaaa atagtccatg aattagtaaa agatacgcag
     3121 gacggaagat ggcagcaagc aaaagaaaag caattaaatg cacttgaact gatccatgcc
     3181 ttgttcagtg agaccaatcc tattcctgtc aaagcagcca tgtcattatt agacttacct
     3241 tccggacctc tacgcctgcc tttagtttct ctttcaaaag agaaaaagaa acaattagct
     3301 cagcttttgt taaaagaaag gacggttgaa aaatgaaaat cggactgatt ggcagtggac
     3361 agatgggaaa tagagtcgaa gaagttgcac aagaaagagg agatacggtc cttcttttac
     3421 ggacggcacc taaagaagca actacgcttg cttttacgga actgccagaa ctgcttattg
     3481 atttttctca tccagataat ttagagaaaa tcctttcgta tagcttatca tatcaacttc
     3541 cgttagtcat tggcacgaca ggttatacac aatcacagtt ccaagaaata aaagaacatg
     3601 ccaaacatgt acctgtgatg tatagtgcta atttttcgtt cggcatcatg gtgatgaatc
     3661 aactaatcaa gcaagcagct gcaatgttgc aaggctggca gatcgaactg attgaaaaac
     3721 atcatagtcg taaaaaagat gcaccatctg gtactgcaaa tatgctgctt caaacaatcc
     3781 aagaagtgca aaagctgcag cccgtataca actggcaagg gaaaaaaaga gaagaaaacc
     3841 aaattggtgt acacagtatt cgtgcaggtt ctttacctgg tgaacatgat gtattgtttg
     3901 ctgctactga tgaaatattt acgatcaaac atgaatcttt ttctaatcga atctttgctg
     3961 caggcgcagt agaagcagcg gactggttga aagatcagtc accaggttat tacaaattag
     4021 aagatttatt aatggacaaa ggggtgtaaa aaacgtggaa gctcaagaaa tcatccgcta
     4081 cattcaaacg gcatctaaaa agacgcctgt gaaagtctat ctgaatgtaa ccgagccaat
     4141 cgaatttaaa gaaagtaaag tttttggtga aggaagcagc ttcgttgttt ttggtgacta
     4201 tgagacgatt gctccggttt tagaagctga atcagaaaaa atcattgaaa tcgaaatcga
     4261 aactgctgca agatacagcg cagttccttt gctagatatt aaaaaaatta atgctcgtat
     4321 cgaaccaggt gctattatca gagatcaagt gtcaatcggc aacaatgccg tgatcatgat
     4381 gggagcgatt atcaatattg gtgcagtaat cggtgaaaac acgatgattg atatgggtgc
     4441 tgtcttaggt ggacgagcga cagtgggcaa aaactgccat attggtgcag gagctgtctt
     4501 agcaggtgtg atcgaaccag catcggccaa accggttatc gtagaagacg gcgtactggt
     4561 aggtgcgaat gcagtgattg ttgaaggtgt ccatatcgga aaagatgcag tcgtggcagc
     4621 aggagcagtg gttttagaag atgtggcagc agaaactgtt gtcggtggaa tacctgcacg
     4681 tgtattaaaa ataagtgatg atcagacaaa agaaaataca gcattgattg ctgcgttacg
     4741 cgaattatag aggagatgga gaaaaatgcg cgaaatagat acctgtattg tgggggcaac
     4801 cggattagtc ggccaaacga tgctcaaagt gttagaagaa tatgattttc cagtaggacg
     4861 attgaaatta ttagcttcaa aacgttcagc cggtaaagtt tgtgtattca agaaaaagaa
     4921 acacactgta caggaattga cagaaacttc atttgaagga tatgatttag cgctattttc
     4981 tgcaggtgct tctgtatcca aagagtttgc gccaatcgca cgaagtcagg gactatttgt
     5041 catagataat tcctcagcat ggcgggaaga ttcggatatt ccattaatcg ttccagaggt
     5101 caatctggaa gactatacat tgaatcggct gattgcaaat ccaaattgtt caaccattca
     5161 agcggtactt ccgcttaaag ctttacaaga agcctttggt atccagcgag tcaattattc
     5221 gacctatcaa gcagtttctg gttccgggca aaaaggcatt gaagatcttc atcgtacgca
     5281 aaatgacgag gaaccagttt tgtatcctta taatattagc caaacggtga ttccggaaat
     5341 cgatcagcct ttggaaaatg ggtatacaaa agaagaacag aaaatgataa atgagacccg
     5401 taagattctg catcagcctg ctttgccagt atcagcaacc tgcgtacgtg tgccaatcga
     5461 aaatggacat ggagtctctg tggcagttca actgcaaaaa gagtttacga cggaagaaat
     5521 tagagagtgt ctcagccagt ttccagggat tcaattagaa gatgatctag cagcacataa
     5581 atatccgacg agcattcttg ctagaggaac agatatggtt tatgttggga ggatcagaaa
     5641 agacacttct ttagagaacg gattgttgtt ctatacgact gccgataaca tacgcaaagg
     5701 agccgctgcg aatgccgtac agattgctgc agctttaatg cagaaaatag cggaggtggc
     5761 aagatgagta atacgctgaa agtagcgaaa ttcggcggaa catcgatgtc tcatgaagag
     5821 caattcaaaa aagtccgtac aattgttctt tcagatccca atcgccaagt gatcgttgtt
     5881 tctgctttag gcaaacgtac gtcagatgat gacaaagtaa cagacttact ttatttgatt
     5941 tatgcttacc ttcagcatca tgttgattgg acaccccttt ggaaaaggat ttgtgatcgt
     6001 ttcattcaag taagagatgt tcttgccctg cagttaccta ttgaagatga actagcacgc
     6061 atacagaaac aactggaaga cgaaaagata actgaagatt atttagtaag tagaggtgaa
     6121 tatctgactg ctcaactgat ggctgagtat cttggttacc agtttattga cgcaaaagac
     6181 ctgattttct ttgactatga tgggaaaatc gatcaggaaa aaacagcaca ggaaatcaca
     6241 aaagcattta aaagatatcc gaaaatggtt gttccagggt tttacggggc gaaccctgca
     6301 ggaaaagaaa aattattggg ccgtggggga agcgacatta ccggcgcgat actggcgaag
     6361 atgttgcaag cggaaagtta tgagaattgg acggatgttt caggaatcat gatggctgat
     6421 ccgcgaatca tcgatcatcc taaaatcatc gaggaaatct cttttagaga actgagagag
     6481 atggcttata tgggggctaa cgttcttcat gaagcagctg ttttcccagt tcaagaggct
     6541 gatatcccca tccaaataaa aaataccaat gatcctttcg ctcaagggac aaaaatatcg
     6601 aatcacgaaa tagaaaagga aaatggactg acaggaatag ctggacgcaa ggattttctt
     6661 tccatcactt tattcaaacg tcatatgtcc gatgaaatcg ggtttatctg gaaagcaatg
     6721 agcatttttg ctaaacatga gatatcaatc gaacatattc cttcagggat cgacaatatc
     6781 ggagtgatcg tctcagcaga agcgattgcc gataagttat ttctcatcac gaaagaatta
     6841 aaagaaacac ttggcgtgga agaaatcgtc gtgatcgaag acttagcttt gatctctgtc
     6901 gttgggggtc cgcataaaga attgattgga ttatctggga aagtgttatc gatattgaac
     6961 aaattagaaa ttcgcacatc gatcttatca caaggagcac aggagttgaa tctgatcatc
     7021 ggtgtaccga ataatcaata tgaaacagta gtgaagggta tctatgaagg gatggtgaat
     7081 acggatgctg aaagaatatc gaatggcact ccatcaaatt cctgaaatcg ggtttcatga
     7141 attcaagaca aaaaaatatt tatatgaaca agtaaaaaac agcgaggccg ttatccatga
     7201 aatcggcgaa acaggactac tcctttattt tgataatcag caagaagaaa cgattgcttt
     7261 tcgaacagat atcgatgcac tgccagtcaa agaagaaacg aatctctctt ttgcttctaa
     7321 gcatgaaggt tatatgcatg catgtggtca tgacggacat atggcgatgc ttttagggtt
     7381 gactgacttt ataactaaac acattgagga tcttatttat aatgtagtac tgatctatca
     7441 gccatcagaa gaaatttccg gtggcgcaca aagcgttatt cagtcaggat tgttggataa
     7501 atatgaagta caagcaatat ttggctttca tctatggcct ggactagcta agggagaagt
     7561 tttctctcga ccaggacctt tgatggcaca aagcagtgaa acagacatta tcattaaagg
     7621 ccagtctgca catattgctt ctagtgaaaa aggaatcgat agtctggagg cagctgtacg
     7681 atttttgaag aatgtttatg attttgaccg gcaattaccg gcagagtgga atcatctaat
     7741 gaaatttggc caaatccaag gtggaacgat ccgaaatgtt ttagctggag aagttattgt
     7801 atccggaagt atccgttctt acagttcaga tatgcaaaat tatttgaaga ggggattaga
     7861 ccatttagca gaacagtttc actctacagc tactatttct tttcgctata atgacggcta
     7921 tccagctgta atcaaccata gaggcttata tgcagcactg ttagattcaa acaaactaca
     7981 tgaattagat gctccagttc ttcaagccga agattttggc atatatacgc aacaatatcc
     8041 atgtgtattt ttctttttag gtgtcggtga tacccctgct ttacatgatc cgacgttcga
     8101 ttttgatatg gatgttttag aaaatggtgt agaatggtac aaaacgattc tttttacaga
     8161 aaatcttctt gtaaaaatcg atagcagttc aaaagaggag cgagtaaata atgatgaatt
     8221 tatccaatcg atttagtcct aggctttcta gaatagaagt atcaaaaata cgacaattcg
     8281 atcagcaaat atcgtcgata ccagatgtga tcaaactcac cctgggagaa ccggattttc
     8341 caactcctga acatgtcaag caagcaggaa tagcagcgat tgaagaagac ttttctcact
     8401 ataccggaat gagaggatta gaggaactgc gggaagcggc ttgtatattt cagcaacagc
     8461 gttatggatt aacttatgat ccgcaaacgg aagttttgac gacagttggt gcaacggaag
     8521 cgattgcctc tgcgttgtta tctgttttgg aagaaggaga caaggttctt attccagcgc
     8581 ctgcttattc aggatatcag ccattagtgg aactagctgg tgctgaattg atcccgatag
     8641 atacttctga cacagggttt gtctgtcagc cggaacaatt tgaacgagca tttgaacaat
     8701 atggatcagc tgtcaaagca gtcattttaa actatcctaa caatcctact gggacgacat
     8761 tgtctgcaaa acaattgaaa gcaatcgctg aggttttaaa aaaatatccg gtatttgtta
     8821 taagtgatga agtatatgct gaattgactt atagtggaac gcatatgtcc atagctactt
     8881 atcttccaga acaaacaatc gtgataagcg ggttatccaa gtcacatgcg atgactggtt
     8941 ggcgagtggg ctttattttt gctcaaaaag cccttattga cgaactaatt aaagtccatc
     9001 aatatctggt gactgccgct acaacgatgt cgcaaaaagc tgcggctgaa gcgctaataa
     9061 atggggtgaa agatagcgag aatatgaaag agcaatatca aacacgaaga gactatctgg
     9121 tccagcaatt ggcgccttta ggatttgaag tgacccagcc aaacggtgca ttttacttgt
     9181 tttgcaaact cccagctgcc attcaaactg actcttggca gttttgtgta gaattagcgg
     9241 aacaggcaaa agttgcgtgt atcccaggaa ttgcatttgg cccagaagga gaaggttata
     9301 tccggatttc ttatgctagc agcatggaaa aattacatga ggcctgcaaa cggatcaaag
     9361 actttttaac agaacagtga ttcatagatt ttatcaaaaa aacaatcgtt aattttgcgg
     9421 tatattctgc aaggagacga ttgtttttta aatttagttc aaatgaacgc gtttcttttg
     9481 attcataact aatgtaatgt taagtttaaa ttgtttagaa tgattttaaa agaatattca
     9541 aggaggtttc atcaatggaa aaaatcaaag ttgtcattgt aggtgcatct catggagggc
     9601 atcaatcgat cttggaacta ttgacacgat acggtgaaaa tgtagatatc acgctatttg
     9661 aagcaggaga ctatatttcc ttcatgtcat gtggtatgga gctgtattta gaagacaagg
     9721 taacagatgt aaatgatgta cgtaatttca gaccagaaaa ctttccgcaa tccaatgttc
     9781 atattttaaa taatcacgaa gtaaaaacga tcgatgccga taaaaaaaca gtaacggtta
     9841 ctagcgcatc agataacgaa acaaaagaat atccttatga caaattgatt ttgagttctg
     9901 gtgtcaaacc aaacaatctg ccagtaccag gagcagatct agaaaatgtc tacttgatgc
     9961 gtggatacaa ttgggcaacc aaaatcaaag aaaaattaaa tgatccggct attaaaaaaa
    10021 tcgctgtcgt aggtgcgggt tacattggga tcgaagcagc agaagcaagc cgaaaagctg
    10081 ggaaagaagt cgtattgctt gatgtgatcg atagaccttt aggcacttat ttagatgctg
    10141 agatgacaga tattttagaa caacacctaa atgaaaacgg tattgaagtt ttgacaaatg
    10201 caaaaatcga agcttttact ggtaacggaa aagttgaggc aatcaaaaca agtgaaaaag
    10261 aaatcccagc agatctagtc attcaagctg ccggtgtgaa ggcaaataca gaatggttaa
    10321 aagggatcgt cgacttagat gaacgtggct ggatccaaac aaatgaatac ttgcaaacga
    10381 atcttccaga tgtgtatgca gtaggagatg ctacacttgc ttattctatc ccagcacgca
    10441 aaaaaatgcc gatcgcttta gcgactgttg cacgtagaga agcacgttat gtagtaaaac
    10501 atctttttga agaagtacca aacaaaccgt tcggcggagt cgtaggttcc tctgcattaa
    10561 gtgtctttga ctatcatttt gcagcaagcg gattgaatag ctttacagca gcaaaagcag
    10621 gagtagatgt acgagtgtct tattatgaag atacgttacg accaaaatat gtaccagaag
    10681 aacaaggaaa tccaaaagtg tccatccaat tattctcgga tccaatgacc catcaattgc
    10741 taggaggagc agtattatct acctatgatg ttacaggtca aggaaatgta ttggcactag
    10801 cgatccaaaa caaactgact ttagaagaac tagcagaagc tgacttcttc ttccagccag
    10861 gctttgaccg acaatggagc atgttgaatc tagccgctca acaagcgtta ggagaagaac
    10921 catttgtaga ataagaaaac gaaatatttg aaaagaggtt gtgaaaaagc tgccttgcat
    10981 caagtaataa gaacagccaa gtaaaaacag cttcttatgt ttttaacttg gctggactta
    11041 ttaccgcaga tgcaagcttt tgaacaccgt ttattttcaa aaaagggact gtgacacgat
    11101 ttgtgtaccg acccccaaaa gttagaccaa aaaatctaac ttttggaggt cggtattttt
    11161 tatggttaaa tatacttttg aacttaaatt aaagatcgtt catgattatt tggatggcaa
    11221 aggcgattgg accgtaaaaa tccagatcaa gagattgaag cggaaatgct taaaattcgg
    11281 gagaagcaca aggattatgg ctgtttacgg atgaccaatg aactaagaaa ccgcggtttc
    11341 tctatcaata aaaagaaagt tcagcgcttg atcaagaagc taggaataaa agtaacggca
    11401 tacacgcgta aatcacgtcg ctacaattct tatcgaggaa aagt
//
LOCUS       DAFBQH010000083        11345 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.086, whole genome shotgun sequence.
ACCESSION   DAFBQH010000083 DAFBQH010000000
VERSION     DAFBQH010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.086"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..936
                     /locus_tag="LK694_002369"
     CDS             <1..936
                     /locus_tag="LK694_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK5053201.1"
                     /translation="GRWKETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESL
                     AIDILTFLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADH
                     IESFTDYDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVA
                     MLIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFV
                     TQNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQC
                     LISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            939..3122
                     /locus_tag="LK694_002370"
     CDS             939..3122
                     /locus_tag="LK694_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBK5053202.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3119..4120
                     /locus_tag="LK694_002371"
     CDS             3119..4120
                     /locus_tag="LK694_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBK5053203.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4135..5049
                     /locus_tag="LK694_002372"
     CDS             4135..5049
                     /locus_tag="LK694_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK5053204.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5294..5410
                     /locus_tag="LK694_002373"
     CDS             5294..5410
                     /locus_tag="LK694_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBK5053205.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5426..7345
                     /gene="tet(M)"
                     /locus_tag="LK694_002374"
     CDS             5426..7345
                     /gene="tet(M)"
                     /locus_tag="LK694_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBK5053206.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7443..8816
                     /gene="tet(L)"
                     /locus_tag="LK694_002375"
     CDS             7443..8816
                     /gene="tet(L)"
                     /locus_tag="LK694_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBK5053207.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9380..10642
                     /locus_tag="LK694_002376"
     CDS             9380..10642
                     /locus_tag="LK694_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBK5053208.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10887..>11345
                     /locus_tag="LK694_002377"
     CDS             10887..>11345
                     /locus_tag="LK694_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBK5053209.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINK"
BASE COUNT         3739 a         1897 c         2376 g         3333 t
ORIGIN      
        1 ggcagatgga aagaaacctt gccagagatt tcccatgaaa tcaatatcgt caatctgact
       61 tctgatgaga aaaacaaagg cttacttgac ccttatgtaa ttatgaaaaa taccaaagat
      121 tctgaatcac tggctattga tattttgaca ttccttacgg ggatttcctc tcgtgatggg
      181 gaacgcttcc caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtgcgaggg
      241 ttgatgaaag tgattgaaga attacgggtt gagaatacgc cactaagtac cagcatagcc
      301 gaccatatcg aaagttttac agactatgac tttgcccatc tgctttttag tgatggttat
      361 gtagagcagt ctatcagcct tgaaaaacaa ctgaacatta tacaggttgc cgacttggta
      421 cttcctgaca aggaaacttc ctttgaggaa tataccacaa tggagttact atcggtagca
      481 atgctgattg tcattagtac ctttgcgttg gactttatcc atacagaccg aagtattttc
      541 aagattgtag acttagacga agcatggagc ttcttacagg tggcacaagg gaaaacacta
      601 tctatgaagc tggttcgtgc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa
      661 aatacagatg acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgccttt
      721 cgttccactg accttaacga gattaaaaaa acccttgcct tttttggtgt agacccagag
      781 gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat
      841 ttatatggtc gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc
      901 tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atattaaaca
      961 gaataaaatc aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca
     1021 cacttgtgat tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct
     1081 tggtagatga tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc
     1141 aactggattt ttatgtggat aatagctggg gctggctacc gtggaactgg tcggacggaa
     1201 ttggaaaaca agtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtc
     1261 tttatgtttc caatgcgacg ggttacttag tacaggaagc ctattcctta gacttcattt
     1321 ccgctacagc agattccatt ggcaagaata tgcagaccct tgcaggggtt acgcctagtg
     1381 gactatcaac agagggtttc tatgttggat tccttttact cttgattttg gttcttgggg
     1441 tttatgttgc ctatacagga ctgataaaga gagaaaccac aaaggcaatt catgccctca
     1501 tgaattttgt gctggttttt atcctatcgg cttcctttat tgcctacgct cccgactaca
     1561 ttaaaaaaat caatgacttt tcatcagaca tcagcaacgc tagtttgtca ctgggtacaa
     1621 aaattgtcat gccccattca gatagtcaag gcaaggacag tgtggacttg atacgtgaca
     1681 gcctattttc catacaggtt cagcaaccgt ggctactgct tcaatataac agttcagaca
     1741 ttgaaagtat tggtattgac cgtgtggaaa gcctgctctc aaccagtcca gattccaaca
     1801 atggggaaga cagagaaaaa attgttgcgg aagaaattga agatagaagc aataccaata
     1861 tgacgattac aaagacgatt aaccgtttag gtacagtctt cttcctattt gtcttcaata
     1921 ttgggatttc catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt
     1981 ttatcatcta tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg
     2041 atggtatgtc aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa
     2101 tcacattgat tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg
     2161 gttatccgtt ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttta
     2221 agctgggcga tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa
     2281 gtcgtatgat gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga
     2341 aacttggacg ctccgtgact gctttagggg ctgggtctgc cattgccagt gctacgggta
     2401 aaaaaggaca gtcgggttcg ggaagttctg cgagtacaca agcagatcac tctcgaccaa
     2461 acgggcagga aaaatcaaca ctaggaaagc gtatcggtca aaccatcggt acagtagctg
     2521 ataccaaaga cagaatggta gatactgctg gtaatttgaa agaacaggtt aaggatttgc
     2581 cgaccaatgc aagatatgca gtatatcaag gaaaatctaa agtgacggat aatgtccatg
     2641 atttaaccag aagtatttct caaaccaaag cggataaagc cagtggacgc aagaaacagc
     2701 aggaacaaag acggaaaacc attgcggagc gtcgctctga aatggaacag gtcaaacaga
     2761 aaaaacagcc agcttcttcc gttcatgaaa gaccagccac taagcaagaa caatctcatg
     2821 atggacagac ttcaaggcaa acaactgtac aggcttccta tagggaatct caacaagcca
     2881 aacaagagcg tccaacagtt aagtccgatt cttcaagttt aaaaacggaa cgtcaaagta
     2941 atacagttca agaaagaacg gttcaaaagc cagtaacttc aactgcacca acagatagag
     3001 cttcacaacg ttcaatcaca aaagaacgtc agtctactgt tcaaagagta ccactacaaa
     3061 atacaaaaag tagaccacca atcaaaaccg ccaccattaa gaaaggcagt aagaaaccat
     3121 gaagctgaaa actttagtga ttggtggttc gggattattc ttgatggtct tctcactgct
     3181 tctgtttgtc gccattttat tttcagatga acaagatggc ggtttttcca acattcacta
     3241 tggcggtgtg gatgtttccg cagaagtgct ggctcataag cctatggtag aaaaagttgc
     3301 caaagaatat ggaattgaag aatacgtcaa tatacttctt gcgattatac aggtggaatc
     3361 gggtggtaca gcagaagatg ttatgcagtc ctcggaatcc ctcggtcttc cacctaattc
     3421 attgagtaca gaagaatcca ttaagcaagg tgtgaagtat ttcagtgagt tattagccag
     3481 tagcgaaagg cttggtgtag atttagaatc ggttatccag tcctacaatt atggtggtgg
     3541 tttcttagga tatgtggcta atcgtgggaa taaatatacc tttgaactgg ctcaaagttt
     3601 ctcaaaagag tattcgggtg acgaaaaagt gtcttacccc aatcccatag ccatacctat
     3661 caatgggggc tggcgataca attacgggaa tatgttttat gtgcaactgg taacgcagta
     3721 tcttgtcaca acagagtttg atgatgatac agtacaagcc atcatggacg aagctctgaa
     3781 atttgaaggc tggaagtatg tctatggcgg agcttccccg actacctctt ttgattgtag
     3841 cggactgaca caatggacgt atggaaaagc tggaatcaac ttaccacgaa cagcacaaca
     3901 gcaatatgat gtaacccggc atatcccatt attggaagca aaagccggcg atttggtttt
     3961 ctttcattct acctataacg ctggctctta tattactcat atcgggattt atttaggcga
     4021 taaccgtatg tttcatgcag gcgaccctat cggttatgcc gacttaacaa gctcttactg
     4081 gcaacagcat ttagtgggag caggacgaat caaacaataa gaaaggacga ttcaatgatg
     4141 aaattcggaa aaaatcagaa tacagaaaaa cagacaccaa aggaaaagaa acctcgtgtc
     4201 tataaggtca atcctcgtaa aaaggttgtg attgtcctat gggtactttt aggacttagt
     4261 ttcagctttg cgatattcaa gcactttaca gccattgata cccataccat tcatgaaacc
     4321 accatcatag aaaaggaata tgtcgatact catcatgtgg aaaattttgt agagaacttt
     4381 gcgaaagtct actattcatg ggaactaaac gacaagtcca ttgataatcg catagaaaac
     4441 ctgaaagcat atctgacaga ggaacttcaa gctctgaatg tcgatacagt tcgtaaagat
     4501 attcctgtat cgtcttctgt aaaaggattt cagatatgga cggtagaagc agatggcgac
     4561 aatcaatttg atgtaaccta cagtgtagac caactcatta cagaggggga aaatacaaag
     4621 accgtccact ctgcttatat agtgagtgtc tatgtagaca gtactggaaa tatggtcctc
     4681 attaagaatc cgaccattac tagcatacca aagaaatcag actataaacc aaaagctatt
     4741 gaaagtgagg gtacggttga ttccattaca accaatgaaa tcaatgagtt tttaacgacg
     4801 ttcttcaagc tctatcctac agcgacagcc agtgaacttt cctactatgt gaatgacggg
     4861 atattaaaac caatcggaaa agagtacatc tttcaagaac tggtaaatcc tatttacaat
     4921 cgtaaggata atcaagtcac ggtatcgctg acagtggagt atatcgacca gcagaccaaa
     4981 gcaacgcagg tatctcaatt tgatttggta cttgaaaaga acgggagtaa ttggaagatt
     5041 atagaataac aaatattggt acattattac agctattttg taatcacgta ctctctttga
     5101 taaaaaattg gagattcctt tacaaatatg ctcttacgtg ctattattta agtatctatt
     5161 taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagtg
     5221 ggaacaaata attggatgtc cttttttagg agggcttagt tttttgcacc cagtttaaga
     5281 atacctttat catgtgattc taaagtatcc ggagaatatc tgtatgcttt gtatgcctat
     5341 ggttatgcat aaaaatccca gtgataagag tatttatcac tgggattttt atgccctttt
     5401 gggcttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
     5461 tgatgcggga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
     5521 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
     5581 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
     5641 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
     5701 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
     5761 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
     5821 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctgccgaaat
     5881 tgtaatcaaa cagaaggtag aactgtatcc taatatgtgt gtgacgaact ttaccgaatc
     5941 tgaacaatgg gatacggtaa tagagggaaa cgatgacctt ttagagaaat atatgtccgg
     6001 taaatcatta gaagcattgg aactcgaaca agaggaaagc ataagatttc agaattgctc
     6061 cttgtaccct gtttatcatg gaagcgcaaa aagcaacata gggattgagc agcttataga
     6121 agtgataacg aataaatttt attcatcaac atacagaaag aagtctgaac tttgcggaaa
     6181 tgtcttcaaa attgaatatt cggaagaaag acaacgtctt gcatatgtac gcctttatgg
     6241 cggaatcctg catttgcggg attcggttag aatatcggaa aaggaaaaaa taaaaattac
     6301 agaaatgtat acttcaataa atggtgaatt atgtaaaatt gataaggctt attccgggga
     6361 aattgttatt ttgcaaaatg agtttttgaa gctaaatagt gttcttggag atacaaagct
     6421 attgccacag agagagagaa ttgaaaatcc gctccctctg ctgcaaacaa ctgttgaacc
     6481 gagcaaacct caacaaaggg aaatgttact tgatgcactt ttagaaatct ccgacagtga
     6541 cccgcttcta caatattatg tggattctac gacacatgaa attatacttt ctttcttagg
     6601 gaaagtacaa atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga
     6661 actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat
     6721 tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct
     6781 tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc
     6841 atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg
     6901 gaatgtgacg gattgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac
     6961 cccagcagat tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg
     7021 aacagaattg ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc
     7081 acgagcatac aacgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa
     7141 taatgaggtc attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga
     7201 tttaactttc tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt
     7261 tactaccggt gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg
     7321 atatatgttc aataaaataa cttagtgtat tttatgttgt tatataaata tggtttcttg
     7381 ttaaataaga tgaaatattt tttaataaag atttgaatta aagtgtaaag gaggaattaa
     7441 ttatgaagtg taatgaatgt aacagggtta aatacaacca aattttaatt tggctttgcg
     7501 ttttatcttt ttttagcgta ttaaatgaaa tggttttgaa cgtctcatta cctgatattg
     7561 caaatgattt taataaacca cctgcgagta caaactgggt gaacacagcc tttatgttaa
     7621 ccttttccat tggaacagct gtatatggaa agctatctga tcaattaggc atcaaaaggt
     7681 tactcctatt tggaattata ataaattgtt tcgggtcggt aattgggttt gttggccatt
     7741 ctttcttttc cttacttatt atggctcgtt ttattcaagg ggctggtgca gctgcatttc
     7801 cagcactcgt aatggttgta gttgcgcgct atattccaaa ggaaaatagg ggtaaagcat
     7861 ttggtcttat tggatcgata gtagccatgg gagaaggagt cggtccagcg attggtggaa
     7921 tgatagccca ttatattcat tggtcctatc ttctactcat tcctatgata acaattatca
     7981 ctgttccgtt tcttatgaaa ttattaaaga aagaagtaag gataaaaggt cattttgata
     8041 tcaaaggaat tatactaatg tctgtaggca ttgtattttt tatgttgttt acaacatcat
     8101 atagcatttc ttttcttatc gttagcgtgc tgtcattcct gatatttgta aaacatatca
     8161 ggaaagtaac agatcctttt gttgatcccg gattagggaa aaatatacct tttatgattg
     8221 gagttctttg tgggggaatt atatttggaa cagtagcagg gtttgtctct atggttcctt
     8281 atatgatgaa agatgttcac cagctaagta ctgccgaaat cggaagtgta attattttcc
     8341 ctggaacaat gagtgtcatt attttcggct acattggtgg gatacttgtt gatagaagag
     8401 gtcctttata cgtgttaaac atcggagtta catttctttc tgttagcttt ttaactgctt
     8461 cctttctttt agaaacaaca tcatggttca tgacaattat aatcgtattt gttttaggtg
     8521 ggctttcgtt caccaaaaca gttatatcaa caattgtttc aagtagcttg aaacagcagg
     8581 aagctggtgc tggaatgagt ttgcttaact ttaccagctt tttatcagag ggaacaggta
     8641 ttgcaattgt aggtggttta ttatccatac ccttacttga tcaaaggttg ttacctatgg
     8701 aagttgatca gtcaacttat ctgtatagta atttgttatt acttttttca ggaatcattg
     8761 tcattagttg gctggttacc ttgaatgtat ataaacattc tcaaagggat ttctaaatcg
     8821 ttaagggatc aactttggga gagagttcaa aattgatcct ttttttataa caggaattca
     8881 aatctttttg ttccattaaa gggcgcgatt gctgaataaa agatacgaga gacctctctt
     8941 gtatcttttt tattttgagt ggttttgtcc gttacactag aaaaccgaaa gacaataaaa
     9001 attttattct tgctgagtct ggctttcggt aagctagaca aaacggacaa aataaaaatt
     9061 ggcaagggtt taaaggtgga gattttttga gtgatcttct caaaaaatac tacctgtccc
     9121 ttgctgattt ttaaacgagc acgagagcaa aacccccctt tgctgaggtg gcagagggca
     9181 ggtttttttg tttctttttt ctcgtaaaaa aaagaaaggt cttaaaggtt ttatggtttt
     9241 ggtcggcact gccgacagcc tcgcagagca cacactttat gaatataaag tatagtgtgt
     9301 tatactttac ttggaagtgg ttgccggaaa gagcgaaaat gcctcacatt tgtgccacct
     9361 aaaaaggagc gatttacata tgagttatgc agtttgtaga atgcaaaaag tgaaatcagc
     9421 tggactaaaa ggcatgcaat ttcataatca aagagagcga aaaagtagaa cgaatgatga
     9481 tattgaccat gagcgaacac gtgaaaatta tgatttgaaa aatgataaaa atattgatta
     9541 caacgaacgt gtcaaagaaa ttattgaatc acaaaaaaca ggtacaagaa aaacgaggaa
     9601 agatgctgtt cttgtaaatg agttgctagt aacatctgac cgagattttt ttgagcaact
     9661 ggatccagga gaacaaaaac gattttttga ggaaagttat aaattatttt ccgaacgata
     9721 tggcaagcaa aatattgctt atgcaacagt tcataatgat gagcaaaccc ctcacatgca
     9781 tttaggtgtt gtgcctatgc gtgatggaaa actgcaagga aaaaatgtgt ttaatcgtca
     9841 agaactgtta tggctacaag ataaattccc cgagcatatg aaaaaacagg gttttgagtt
     9901 gaagcgtggt gaacgtggct ctgaccgtaa acatattgag acagctaaat ttaaaaaaca
     9961 aactttggaa aaagagattg attttctaga aaaaaattta gcagttaaaa aagatgaatg
    10021 gactgcttat agcgataaag ttaaatcaga tttagaagta ccagcgaaac gacacatgaa
    10081 aagtgttgaa gtgccaacgg gtgaaaagtc catgtttggt ttgggaaaag aaataatgaa
    10141 aacagaaaag aaaccaacca aaaatgttgt tatatcggag cgtgattata aaaacttagt
    10201 gactgctgcg agagataacg ataggttaaa acagcatgtt agaaatctca tgagtactga
    10261 tatggcgaga gaatataaaa aattaagtaa agaacatggg caagttaaag aaaaatatag
    10321 tggtcttgta gagcgattta atgaaaatgt aaatgattat aatgagttgc ttgaagaaaa
    10381 caagtcttta aagtctaaaa taagcgattt aaagcgtgat gtgagtttaa tctatgaaag
    10441 cactaaggaa ttccttaagg aacgtacaga cggcttaaaa gcctttaaaa acgtttttaa
    10501 ggggtttgta gacaaggtaa aggataaaac agcacaattc caagaaaaac acgatttaga
    10561 acctaaaaag aacgaatttg aactaactca taaccgagag gtaaaaaaag aacgaagtcg
    10621 agatcaggga atgagtttat aaaataaaaa aagcacctga aaaggtgtct ttttttgatg
    10681 gttttgaact tgttctttct tatcttgata tatatagaaa taacgtcatt tttattttag
    10741 ttgctgaaag gtgcgttgaa gtgttggtat gtatgtgttt taaagtattg aaaaccctta
    10801 aaattggttg cacagaaaaa ccccatctgt taaagttata agtgaccaaa caaataacta
    10861 aatagatggg ggtttctttt aatattatgt gtcctaatag tagcatttat tcagatgaaa
    10921 aatcaagggt tttagtggac aagacaaaaa gtggaaaagt gagaccatgg agagaaaaga
    10981 aaatcgctaa tgttgattac tttgaacttc tgcatattct tgaatttaaa aaggctgaaa
    11041 gagtaaaaga ttgtgctgaa atattagagt ataaacaaaa tcgtgaaaca ggcgaaagaa
    11101 agttgtatcg agtgtggttt tgtaaatcca ggctttgtcc aatgtgcaac tggaggagag
    11161 caatgaaaca tggcattcag tcacaaaagg ttgttgctga agttattaaa caaaagccaa
    11221 cagttcgttg gttgtttctc acattaacag ttaaaaatgt ttatgatggc gaagaattaa
    11281 ataagagttt gtcagatatg gctcaaggat ttcgccgaat gatgcaatat aaaaaaatta
    11341 ataaa
//
LOCUS       DAFBQH010000084        11253 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.087, whole genome shotgun sequence.
ACCESSION   DAFBQH010000084 DAFBQH010000000
VERSION     DAFBQH010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.087"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(40..1419)
                     /locus_tag="LK694_002378"
     CDS             complement(40..1419)
                     /locus_tag="LK694_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HBK5053210.1"
                     /translation="MTKVTLKKVNPPKKDNEAKIYQLTDETMKFRKEKVLAAMEKSEI
                     DTLVIYCDLEHGGNFSYLTGFVTRFEESLLVLHQTGEAFLVLGNENTKMVDYSRIEAE
                     LIHTPLFSLPDQPMDNEKPLTEVLTNAKISKGKKVGLVGWKMFTSEEDNYQIQDLPAF
                     IVSSIVELVGEDAVSNRTDLFIHSDYGVRSLNTANEVAYYEFGSSLASDCVMNAIDEV
                     EVGKTEMEIGNHLVKYGQPNNVIPIAATGERFENAYIYPTDKQVKLGDKMSITTGFKG
                     GLASRSGYAVATTDDLPEGQKDYIEKVAAPYYDAVVAWLENIKIGMQGKELYSVIGKV
                     LPKSEYKWHLNPGHLTADEEWMSSPMKKDSEITLKSGMLLQIDIIPSVAGYAGASCES
                     GIALADESLRDQLAKEYPNVWSRIISRQEYIRNELGIELPDEVLPLSSGVGYYTPFFL
                     EKNLALVKG"
     gene            complement(1440..2987)
                     /locus_tag="LK694_002379"
     CDS             complement(1440..2987)
                     /locus_tag="LK694_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK5053211.1"
                     /translation="MGPLSIIVASGLLLGIVSILQNPTLVGESFANATVIQGFVGGVQ
                     AIVGTMFGLLPILFAISVAIGMSREDKEIAAFSVVIAFILFHVVINYLLQLRGITAES
                     TSVETLMSDGMSQIKAVETSNMYETLLGTFTYRMGVFGGIIAGLWTAMIHNRYHTKQL
                     PAAFSFFSGNRFVPIMIILTIPIISIASFFIWPFVNTAINALGGLIGKSGVIGTFLYG
                     FSERLLIPTGLHHVLNQLIRFTPIGGTATINGETVSGALTIFNTELALPVKDMDILRS
                     ATRFLTQGTHPFMVFGLPAACYAMYKTSYLAQRPKIKGMLIAAAATCFVTGITEPIEF
                     AFIFISPILWVFHAFMAGLSFMLMNLFGVAVGNAGGGFIDLAIFGILQGTYTRWYLVV
                     ALGLVYAVAYYFVFKYVIERFDVKTPGRSNDDVLEDQDEEMTENELGKLILQGLGGAG
                     NIQEIDNCISRLRLVLKDTATIDEHILKKTGSMGIVKINENNLQVVYGGQVENATRSL
                     KNEMKRK"
     gene            complement(3236..4015)
                     /locus_tag="LK694_002380"
     CDS             complement(3236..4015)
                     /locus_tag="LK694_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK5053212.1"
                     /translation="MTLEQLVNEHHSKLNENDDYVLSFITQNMEISISQTVSELAKQA
                     NVSDSTIIRLTKKLGFKGYGEFRYYLKEEFARMQKTRVDKSSLFLPEVLLGDVQATTK
                     LFEDDKSVEDIYETIRKADKIFAYATGYGQRMMLNEFARCMWNLGIYIIVVPEKNELD
                     LISKALTSNDLLFVISLSGRVDRIDDTLRRIHMKGTQLVSVTRFGQNNLASLSDYSLY
                     YQVTNINDVNELNNSSFCTLNLALSLLYEGYINYYKQSSVD"
     gene            complement(4090..4548)
                     /locus_tag="LK694_002381"
     CDS             complement(4090..4548)
                     /locus_tag="LK694_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK5053213.1"
                     /translation="MGFFSRKEKLVAPVSGNLIPLSQVSDPVFSQGMMGEGFAVEPTN
                     AKVVAPIDGVIKSIFPTKHALTLTSNQGRDLLIHIGIDTVQLKGVGFQVLVEEGQRVS
                     SGESLVIFDCDQIKAEKLSDVVMVLFPEDKGIKLEVSEKKVSEGELLFKI"
     gene            complement(4560..5834)
                     /locus_tag="LK694_002382"
     CDS             complement(4560..5834)
                     /locus_tag="LK694_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBK5053214.1"
                     /translation="MEDLIMKTNFERIKKDIENLAEYNATPGEGVTRSAYSKEDREAR
                     NYLINQMEQLGLEIWEDGFSTLFGRRAGKKADAPSVMIGSHYDSVVNGGRFDGVAGVV
                     TALETMRILEENNFENDYPIDLILMNAEEGETFGPSTGVSNSRAMMGTLTMSELETVK
                     NRHGQTKLEALRAYGVEPDLEAAKRDPKTIKNFIELHIEQGPVLEDEGKDIGLIEFIP
                     GIGRFKARFYGETEDSTAPMNKRKDALVAASKFVLFVEQWMKNLGEGIAGTVGKLTIK
                     PNSNQFVPDFVEALIEIRTFDTKTTNPQEIKEKLLNELSVIQGETGVRTELEEMARIN
                     YSNPTNPSVMDQGNVEKMKHICDQLGYSYQVINNGTGHDAMMMTDFVPTNMIYIPSKG
                     GITHHPDEWTDFEDVKKGADVLLNLVMDISKD"
     gene            complement(5935..6249)
                     /locus_tag="LK694_002383"
     CDS             complement(5935..6249)
                     /locus_tag="LK694_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK5053215.1"
                     /translation="MDYEIQKFISEKEDQYFDRKSARIKPRDIIKHIVAFANADGGKL
                     VIGIEDDGTISGFKFQGAIDPWICVNKVDAFIESFLNQSTLELCYETRKSSYGGIYDN
                     DF"
     gene            complement(6395..7891)
                     /locus_tag="LK694_002384"
     CDS             complement(6395..7891)
                     /locus_tag="LK694_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HBK5053216.1"
                     /translation="MNHQEAKQFFLKTSSFFSTSLPNYIDLSVGIEKAVDLLKNKPFT
                     ELVRHKRSLSEAVDVNHRILANKDGNYAWRPLQILHPLVYVDLVNGITQKNNWNHIKN
                     KFEEFQADKRIECISLPVESISSKSDTAETILNWWENLEQAQISYALEYDYCIHSDIT
                     DCYSSMYTHTIPWAVHSKEWAKTHRKDTQGVGNLIDSKIRYLQNGQTNGIPQGNVLMD
                     FVAEIVLGYADKMLLNKIEKTEIKEFKILRYRDDYRIFSVRKDQAEVIIRLLSEVLSD
                     LNLKLNSNKTFLSDNIILDAIKPDKIYWDLKHASFTEKSNNKWKFKLSLQKHLLQVKL
                     LGDKYPNCGSLSKALSEVYTHRVSTLNKRPDDIYQLISILVNIMQKNPRTLEACIVIL
                     GKLFEFIAVEEINLYLDKILRKFVNTPNTDIVEIWLQRLSLIHSREKSYSAKLCKKVS
                     NPKGFTLWNSDWLKKGFDESGIVNETFISNLSLTTPAKDLELFISQYE"
     gene            complement(8069..8548)
                     /locus_tag="LK694_002385"
     CDS             complement(8069..8548)
                     /locus_tag="LK694_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBK5053217.1"
                     /translation="MKVYTVQPVDFLQSVDSEGFIYPKKRYTELSVFFDNPNAVGALH
                     WLEEEYTKRIQLPLDRDLIWLWRDTCHLKYARKDNFTDFSKYNFYCFEIPIEFYRKNI
                     LWSRFIEWHEPLNHEWETDENQEFEHIFDIPKHPGYGDIQGVTTRLHKDWIKKITHN"
     gene            8715..8864
                     /locus_tag="LK694_002386"
     CDS             8715..8864
                     /locus_tag="LK694_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053218.1"
                     /translation="MRKVDEYIKKKSQKSKVWQRTFELEYKKLSDSLKKDTYNPNEQS
                     KADNQ"
     gene            complement(8924..9169)
                     /locus_tag="LK694_002387"
     CDS             complement(8924..9169)
                     /locus_tag="LK694_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5053219.1"
                     /translation="MLFLKEEKLIDKENVIGSNIRRIRLEKGMGQTELVRELQLSKIA
                     MTRETLVKIEGGRQHIKLDQLRAIRDILQVSYEDLLQ"
     gene            9274..10047
                     /locus_tag="LK694_002388"
     CDS             9274..10047
                     /locus_tag="LK694_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053220.1"
                     /translation="MKKFAIILTCALLTGAISGCATSDTNSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSESNSIEKVLPIEIEKNQENIKLKAELPILNSTEIDW
                     HENIKVTIEKGALAQLYTEKVEQFFESGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMTLKKIKAYDHPLNLTLNNGLLSFVLAGYVDYEYSPDYLETPLQHENNDINFR
                     FVYESKSKTWLVDQLSTINTSFHELPKPENEADMVIKIF"
     gene            complement(10276..10524)
                     /locus_tag="LK694_002389"
     CDS             complement(10276..10524)
                     /locus_tag="LK694_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBK5053221.1"
                     /translation="MDTITPTNFKKNIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDLMKNEKEDDFKVV"
     gene            complement(10623..10937)
                     /locus_tag="LK694_002390"
     CDS             complement(10623..10937)
                     /locus_tag="LK694_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053222.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFRLFRDQIIGISQT
                     TK"
     gene            complement(11074..11220)
                     /locus_tag="LK694_002391"
     CDS             complement(11074..11220)
                     /locus_tag="LK694_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053223.1"
                     /translation="MILNNPEYIESGKAETVNYYYELPSEIQNYQIVIREADGYFVGL
                     PFTL"
BASE COUNT         3500 a         2316 c         1684 g         3753 t
ORIGIN      
        1 tcatttcttc cgttttaaag aagaaatgat tttcatagat caacctttaa ctaaagctag
       61 attcttttct aaaaagaatg gtgtgtagta gcccacaccg ctggacaacg gtaaaacttc
      121 atccggcaac tcgatgccca gttcgttacg gatgtactct tggcgagaaa tgattcgcga
      181 ccaaacattt ggatattctt tggctagttg atcacgaagc gactcatcag ctagcgcaat
      241 cccactctca cagcttgcgc cggcatagcc agcaactgat ggaataatat caatttgtag
      301 taacattccg gacttaagag taatttctga gtcttttttc attggcgaag acatccactc
      361 ttcatcagcc gtaagatgcc ccggatttaa gtgccatttg tattccgatt taggcaagac
      421 tttgcctatt actgaatata gctctttacc ttgcattcct attttgatat tttccaacca
      481 tgcgaccacg gcgtcgtagt atggagctgc aactttttca atgtaatcct tttgcccttc
      541 tggtaggtca tctgtcgttg caactgcata gccacttcga cttgctaatc cgcctttaaa
      601 gcccgtagta attgacattt tatcgcctag tttaacttgt ttatcagttg gatatatata
      661 ggcgttctca aaccgttccc cagttgcagc aattggaatg acattatttg gttggccgta
      721 cttaactaag tggttaccaa tttccatttc tgtttttcct acctctactt catcaatagc
      781 gttcatcaca caatctgaag ccaaggatga gccaaattca taatatgcca cttcattagc
      841 agtatttaat gagcgtactc cgtaatcact gtgaatgaac aggtcggtgc gattgctaac
      901 ggcatcttcc cctactaatt ctacaatcga acttacaatg aatgccggca aatcttggat
      961 ttggtagtta tcctcttctg aagtaaacat cttccaacca acaagcccaa ctttcttacc
     1021 tttactgatt ttagcattgg tcaatacttc agttagtggt ttctcgttat ccatcggttg
     1081 atccggtaat gagaagagtg gcgtgtggat taattctgcc tcaattcttg aatagtccac
     1141 cattttagta ttttcatttc ctagaactaa gaaagcttca cccgtctgat gtaataccaa
     1201 caagctttct tcaaagcgag taacaaaacc tgttaaataa gagaagttcc cgccatgttc
     1261 caaatcacaa tagattacga gggtatctat ctctgacttt tccatcgctg caagtacctt
     1321 ttctttacgg aacttcattg tttcatcagt caattgatat attttagctt cattatcttt
     1381 tttaggtggg ttaacttttt ttagagttac cttagtcata ttgtcactac tttctgtttt
     1441 tatttacgct tcatttcatt tttcagagat cgagttgcgt tttccacttg tcctccatat
     1501 accacttgta aattattttc gttaatttta acgattccca ttgagccggt ctttttcaag
     1561 atatgttcat caattgttgc tgtatcttta agaactagtc tcagccgaga gatacagtta
     1621 tcgatttctt gaatattacc agcaccacca agtccttgca agattagttt gccaagttcg
     1681 ttttctgtca tttcttcgtc ctgatcttcc aacacatcat cgttggatcg accaggagtt
     1741 ttcacatcaa atcgctcaat gacatactta aatacaaagt aatatgcaac tgcataaaca
     1801 aggcccaatg ctacaactaa gtaccaacga gtataagtac cttgtaaaat tccgaagata
     1861 gccaaatcaa taaatccacc accggcattc ccaactgcga ctccgaatag attcattaac
     1921 ataaaggata aaccagccat gaatgcatgg aagacccata gaatcggtga gataaagata
     1981 aaggcgaatt caattggctc agtaatcccg gttacaaaac aagtagccgc tgctgcaatc
     2041 aacatccctt taatctttgg acgttgcgcc aaatatgaag ttttgtacat tgcatagcat
     2101 gctgccggca atccgaagac cataaatgga tgagtacctt gtgtcaagaa gcgagtagca
     2161 cttctcaaaa tatccatgtc ttttactggc aatgctaatt cagtattgaa gatggttaat
     2221 gctccagaaa ctgtttcacc attgatagtt gccgtaccgc cgattggtgt aaatcgaatc
     2281 aactggttta gtacatggtg aagacctgtt ggaatcagca ggcgctctga gaagccatat
     2341 aaaaaagttc caattacgcc tgacttacca atcaaaccgc ctaaagcatt gatagctgta
     2401 ttgacaaaag gccagatgaa aaatgaagcg attgagatga ttggaatagt taggatgatc
     2461 ataattggaa caaaacgatt gccactaaag aaactgaaag ctgctggtaa ttgttttgtg
     2521 tgatagcgat tgtggatcat ggctgtccat aatcctgcaa tgataccgcc gaaaacaccc
     2581 atacgatatg tgaatgtgcc aagtaaagtt tcatacatat tggaagtttc tactgctttg
     2641 atttgactca ttccgtcaga catcaatgtt tcaacagaag tgctttccgc tgtgatgcct
     2701 ctaagctgaa gcagataatt gataactaca tggaacagaa taaatgcaat aaccactgaa
     2761 aatgctgcaa tttccttatc ttctctagac atcccgattg ctactgaaat agcaaataga
     2821 ataggaagta gtccaaacat cgtaccaaca atagcctgta ctccaccaac aaatccttgg
     2881 atcacggttg cattagcgaa gctttctccc accaaagtag gattttgtaa aattgaaacg
     2941 attcctagta gtagtcccga tgcgacaata atagacaacg gacccattaa tgatttccct
     3001 aacttctgga tataagttct caatataaac accttcttct ttttagttga tataaacgtt
     3061 tacatccctt gtacaacgaa agtatagcac cctgaaaaag cgctaacaat acgataggtc
     3121 tgataaaaat caacattcag cacttggttg ttcattcaaa acgtgaatct tcaatcacag
     3181 aaaaatacgc gctatcaagc acgtatttta ggtgtaaata tatgcatcaa ctttattaat
     3241 caacgctact ttgtttgtaa tagttaatat acccttcata cagtaagcta agagcaagat
     3301 tcaaggtgca gaatgatgag ttgttcaact cattcacatc attaatgttg gtcacttgat
     3361 agtataggct atagtctgac agactggcta agttattttg accaaatcgt gtaactgaga
     3421 ccaactgtgt ccctttcata tggattcttc ttaaagtatc atcaatccga tcaacacgtc
     3481 cggacaatga tatgacaaat agtaaatcat tcgaggtcaa agcttttgaa atcaggtcta
     3541 attcattctt ttccggtaca acgataatgt agattcccaa gttccacatg cagcgtgcaa
     3601 actcattgag catcattctt tgtccatatc ctgttgcgta ggcaaatatc ttatctgcct
     3661 ttctaatcgt ttcatatata tcctcaacac ttttatcatc ttcaaacaat ttggttgtcg
     3721 cttgaacatc tccgagtaat acttccggta aaaacaaact acttttatcg actctggttt
     3781 tttgcatgcg tgcaaattcc tctttaaggt aatatcgaaa ttcgccatat cctttgaatc
     3841 cgagtttctt agttagtcta ataatcgttg agtcagacac atttgcttgt tttgctaatt
     3901 ccgatacagt ttgactgatg ctgatttcca tattttgagt gataaaactt aatacataat
     3961 catcattctc attcaattta ctgtgatgtt catttactaa ttgctccaat gtcataatca
     4021 gacctccaaa aataataaaa aagattgctc tctcatttta atacgaaagc acaatctttt
     4081 taagtactat taaattttga aaagaagttc tccttcagaa accttttttt cagagacttc
     4141 tagtttaata cccttatctt cgggaaatag aaccataaca acatctgaca gtttttccgc
     4201 cttgatttgg tcacaatcaa aaatcaccaa tgactcgccg gatgatactc gttgaccttc
     4261 ttcaacgagc acttgaaacc cgactccctt taactgaaca gtatcaattc caatatgaat
     4321 caataaatct ctaccttgat tactagtcaa tgtgagtgca tgctttgttg gaaatatcga
     4381 tttaatgaca ccgtcaatcg gtgctaccac cttagcgttt gttggttcaa cagcgaatcc
     4441 ttcgcccatc atcccttgcg aaaatacagg atcgcttact tggctaaggg gaatcagatt
     4501 tcctgatact ggagcaacta atttttcttt tcgactaaaa aatcccattg acttactcct
     4561 taatcttttg aaatgtccat cacaaggttt aaaagaacat ccgccccttt tttgacatcc
     4621 tcaaaatcag tccactcatc aggatgatgt gtaatgccac ctttggatgg aatataaatc
     4681 atatttgttg ggacaaagtc ggtcatcatc atcgcatcat gtccagtacc attgttaatg
     4741 acttgatagc tatagcccaa ctgatcgcaa atatgtttca tcttctcaac attaccttga
     4801 tccataacac ttggatttgt aggattactg tagttaattc gagccatttc ctccagctca
     4861 gttctaactc cagtttcccc ttgaatcact gataactcat tcaacaactt ttctttaatc
     4921 tcttgaggat tagttgtctt tgtatcaaag gttctgattt cgattaaagc ttcgacaaag
     4981 tcagggacaa attgatttga gttcggtttg attgtaagtt taccgacggt tccggcaatc
     5041 ccttcaccaa gattcttcat ccactgttca acaaaaagaa caaatttaga agcagcaact
     5101 aaagcatctt tccgtttatt cattggagca gtcgagtcct cagtttctcc ataaaaacgt
     5161 gctttaaaac ggccgatgcc aggaataaat tcaatcaatc cgatatcttt gccttcatct
     5221 tccaggactg gtccttgttc gatatgaagt tcaataaaat ttttgattgt ttttggatct
     5281 cgcttagcgg cctctaaatc tggttccact ccataagcac gaagagcttc caattttgtt
     5341 tgtccatggc gattttttac tgtttccaac tcactcatgg ttaatgttcc catcatagct
     5401 cgtgaatttg agacacccgt acttggccca aatgtctcgc cctcctctgc attcatcaga
     5461 attaggtcaa ttgggtaatc attttcaaaa ttattttctt ccagaatccg cattgtttcc
     5521 agtgcagtaa caacgccagc cactccgtca aatctgcctc cattaacaac tgaatcataa
     5581 tgagagccaa tcatcactga tggtgcatca gcctttttac cggctctacg accaaataat
     5641 gtagagaagc cgtcttccca aatctctaaa cccagttgtt ccatttgatt gatcaaataa
     5701 tttcttgctt cacgatcttc tttagagtaa gcagagcggg tcactccttc acctggagtt
     5761 gcattgtact cggctaaatt ttcgatatcc ttttttatac gctcaaaatt tgtcttcata
     5821 attaaatcct ccatttccta caagtgaatg ttagcactca tagaaagcgg ctacaatata
     5881 atcaatgaag attgaatcga tattcgaaaa ctgaatattg cttcggttaa tgacttagaa
     5941 atcattatcg tagattccac cataagaact ttttctcgtc tcataacaca actcgagagt
     6001 ggattggttt aaaaaactct cgataaatgc gtccacttta ttgacacaga tccatggatc
     6061 gatcgcacct tgaaatttaa acccagaaat agtcccatca tcttctatac cgattaccag
     6121 cttacctcca tcggcatttg caaacgctac tatgtgtttt ataatatctc taggcttaat
     6181 ccgtgcactt tttctatcaa aatactgatc ttctttttca cttataaact tttgaatctc
     6241 ataatccatt taaaatttcc cttcttaaat ttttttatta tagagccgta tattcaattt
     6301 ccacaaaaaa ataatctaaa acaaaacaga caccttcatt ttgatatgtt ctcccacata
     6361 tgaatcggtc gctatacagc attacatata gtaattattc atactgcgaa ataaatagct
     6421 ccaaatcttt agctggcgta gttaagctta aattcgaaat gaaagtttca ttgactattc
     6481 cactttcatc aaatcccttc ttcaaccaat cagagttcca caacgtaaat cctttaggat
     6541 tagaaacttt cttacataat tttgcactat aggatttctc tcttgaatga attaaagaaa
     6601 gtctttgtaa ccaaatttca actatatccg tattaggagt attaacaaat tttcttaaga
     6661 ttttatccaa gtataagttg atttcttcta cagcaataaa ctcaaaaagc ttacctaaaa
     6721 tcacaataca agcctccaaa gttctaggat tcttttgcat tatattaaca agaatactaa
     6781 tcaattggta gatatcatct ggtcttttgt ttaaagtaga aactctatgt gtatagactt
     6841 cacttagcgc tttacttaaa ctcccgcaat ttggatattt gtctcctaaa agctttacct
     6901 gtagcaaatg cttttgaagg ctaagcttaa acttccactt attattactt ttctctgtaa
     6961 aacttgcatg tttaagatcc cagtaaattt tatcaggctt aattgcatct aaaattatat
     7021 tatccgataa gaatgtttta tttgaattta gcttcaaatt tagatccgat aatacttcag
     7081 ataacaatct gataatcact tcagcttgat cttttcgaac cgaaaaaatt ctgtaatcat
     7141 ctctgtaacg aagtattttg aactctttaa tctctgtttt ttctatcttg ttcagtaaca
     7201 ttttatcagc atacccaagt actatttcag ctacaaaatc catcaataca tttccttgag
     7261 gtataccgtt cgtttgtcca ttttgtaaat atcgtatttt agaatctatt aagtttccaa
     7321 cgccttgagt atcttttcta tgtgtcttag cccattcttt tgaatgtact gcccacggga
     7381 tagtatgtgt gtacattgag ctataacaat cggtaatatc tgaatgaatg cagtagtcat
     7441 actccaaagc ataactaatc tgtgcttgtt caaggttctc ccaccaattt aaaatagttt
     7501 ccgcagtatc gctctttgaa gaaatagatt ccacaggaag tgatatacat tcaattcgct
     7561 tgtcggcttg gaattcttca aacttatttt tgatgtgatt ccaattattt ttctgtgtaa
     7621 tcccgtttac taagtctaca tatactaatg gatgtaatat ttgaaggggt ctccaagcat
     7681 aattaccatc tttattagct aatattcgat gattaacatc aactgcctca gagagcgatc
     7741 tcttatgtct aactagttca gtaaatggtt tgttttttaa taaatccact gctttctcaa
     7801 tgcctactga taaatcaata tagttgggca acgatgtact aaaaaaactt gatgttttta
     7861 aaaagaattg ctttgcttct tgatgattca tctctaaaat actttttcta gtcctagtca
     7921 aattcaaaat ctcctagatc aattaagttg taatgtaaga ggatacccta aggttacaca
     7981 ccctatttac tattatacca gtattcattc gtgatacata agggtgtaac attaagaact
     8041 ctatctaaaa gactacaagt agtcaccttt aattgtgagt aattttcttt atccaatcct
     8101 tgtgcaacct tgtcgtcacc ccttgtatat ccccgtaccc cggatgtttc ggaatatcaa
     8161 aaatgtgctc aaattcctga ttttcatcag tctcccactc atgattcaac ggttcatgcc
     8221 actctataaa ccttgaccat aaaatgttct ttctataaaa ctcaatagga atttcaaaac
     8281 agtaaaaatt gtatttgcta aaatctgtaa aattatcttt acgagcatac tttaaatggc
     8341 aggtatctcg ccacaaccaa attaaatctc gatccaatgg taactgaatt cgtttcgtat
     8401 actcttcttc aagccaatga agtgcaccaa ctgcattcgg attatcgaaa aagacagata
     8461 attcagtata gcgtttcttt ggataaataa atccttctga atcaacagat tgcaaaaaat
     8521 ctactggttg aaccgtgtac actttcattt attcgccacc tttttttgac tacttatagt
     8581 catttaccaa tttgaaatct gcatactttt tcattcaatt gagcccagca ttaattgtac
     8641 acgagttttc ttcactaacg gatagtatca ggatatacta atactatcct gaatagcgaa
     8701 atgaggtagc caaaatgaga aaagtcgatg aatatatcaa aaagaaatcc caaaaaagta
     8761 aagtatggca aagaacattt gagttagagt ataagaaact ctcagatagc ctcaaaaagg
     8821 acacttataa tccaaatgaa caaagtaaag cagataacca ataaagatta ccctcccata
     8881 tttaacattg cttcacgaat cagtaatcaa tgactacaag tagtcattga agcaaatctt
     8941 cataagatac ctgtagaata tccctaatag ctctcaattg atccagctta atatgttgcc
     9001 ggccaccttc aatcttaacc aatgtttctc ttgtcatagc gattttactg agctggagtt
     9061 ctcgcaccag ttctgtctgt cccattccct tttctaatcg gattcgtctg atattgctac
     9121 cgattacatt ttctttatct attaactttt cttccttcaa aaatagcacg ccttttggac
     9181 taaaattagt cctttttctt attaatctta aagatttggt atgctacaat gggactagta
     9241 ttagtccata ttcaatttta aggaggaatt tttttgaaga aatttgcaat cattctaacc
     9301 tgtgctttac tcacgggagc tataagtggg tgcgccactt ctgacacaaa ttctacaagt
     9361 actgaaacaa gtacaacaac ctcagaaaag aattatccta tacatttttt tccagttcca
     9421 gatggatttg aagcttatta tcatgataaa aaactagatg ttccattgtc agaaagtaac
     9481 tctattgaaa aagttttgcc aattgaaatt gaaaaaaatc aagagaatat aaaactcaag
     9541 gccgagcttc ccatactgaa cagcacagaa attgattggc acgagaatat taaagtaact
     9601 attgaaaaag gagcgttagc acaactttat actgaaaaag ttgaacaatt ttttgagtct
     9661 ggtgccaata aagacttttc aaaaataagc gattattcgg ataacttttt taatgagacc
     9721 caagatgaaa tcacgaactc aacttttatg gctgaagata tgtacaatat gacgttaaaa
     9781 aaaataaaag cttacgacca tccattaaat ctgacactaa ataatggatt actctctttt
     9841 gtattagcag gttacgttga ctatgaatat tcaccagatt atttggagac acctcttcaa
     9901 cacgagaaca atgatattaa ttttagattt gtctacgagt caaaaagtaa aacatggtta
     9961 gttgatcaat taagcactat aaacacaagc ttccatgaat tacctaaacc tgaaaatgaa
    10021 gctgatatgg tcattaaaat cttctaaaaa agaagcgttg aaggagaaat attcctttca
    10081 acgctctttt ttatatgctt agaaacataa ataaattgta tactttcttt ttagccccta
    10141 ttacttttca cgaaaaatat cttttttcga cactagcgtt tctctactta taatattgta
    10201 cacagttttt gtattattga aaaattactc attatttagc attaatccac atcgttttat
    10261 caatcgacct tcatactaaa ctactttaaa gtcatcttcc ttctcatttt tcattaaatc
    10321 agatacataa gcccatgtac ccgttcgttg taaataaagt tcttcttgta gcagctccca
    10381 ctttctctta gaaatcataa tgacgccatc gttcaatcca tttttagaat ccctagtaat
    10441 ttctatttca gaatttgaat gaactacttc ttgcaacact ttacctatat tttttttgaa
    10501 gtttgttgga gtaatagtat ccaaaatgaa cacctctctt ctctatacct ctattatgaa
    10561 gcgcccatca aataaagcaa gcgttgagga ataattcttt caacgcttgc tttattaagc
    10621 tttcattttg tagtttgact aataccaata atctgatctc taaacaatcg aaaaacttct
    10681 tgtggttgat aataatctgt atctatttca aatgtttctg agaacaattc attgaatttt
    10741 tctactgaaa tgatgatttt accattctcc tctcgactgt tttctattat ttctgcttct
    10801 aattcaaata aatctagtga cgaactaaaa tcataaatcc tgtcatattg aacaaaaaaa
    10861 tcaagaaaaa gttgctcaaa ctcttcctta tttatcacta gtgtttcaag cggaacaact
    10921 ttattttcat cagccattct tccaatttcc tcactttcat tttcaaaaaa tcgtaaaatt
    10981 gatatagatt ctttgtctct aatcactcgt gatattatct tgacagtcct acatctaatt
    11041 caattaaagt ataagccact ttcaaataaa agattacaaa gtaaatggca agccaacaaa
    11101 ataaccatct gcttcacgta ttacgatttg ataattctgg atttcgctcg gaagttcata
    11161 ataataatta acagtttctg ctttgccact ttcaatatat tctggattat tcaatatcat
    11221 agcatactca taaagttctt caccattttt att
//
LOCUS       DAFBQH010000085        11097 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.088, whole genome shotgun sequence.
ACCESSION   DAFBQH010000085 DAFBQH010000000
VERSION     DAFBQH010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.088"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            35..658
                     /locus_tag="LK694_002392"
     CDS             35..658
                     /locus_tag="LK694_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053224.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /locus_tag="LK694_002393"
     CDS             1107..2156
                     /locus_tag="LK694_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK5053225.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="LK694_002394"
     CDS             2153..3034
                     /locus_tag="LK694_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5053226.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="LK694_002395"
     CDS             3037..3807
                     /locus_tag="LK694_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5053227.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="LK694_002396"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="LK694_002396"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBK5053228.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="LK694_002397"
     CDS             5118..6281
                     /locus_tag="LK694_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBK5053229.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="LK694_002398"
     CDS             6398..6808
                     /locus_tag="LK694_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK5053230.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="LK694_002399"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="LK694_002399"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBK5053231.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9381..9665
                     /locus_tag="LK694_002400"
     CDS             9381..9665
                     /locus_tag="LK694_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053232.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9825..10061
                     /locus_tag="LK694_002401"
     CDS             9825..10061
                     /locus_tag="LK694_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK5053233.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10058..10456
                     /locus_tag="LK694_002402"
     CDS             10058..10456
                     /locus_tag="LK694_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBK5053234.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3876 a         1833 c         2308 g         3080 t
ORIGIN      
        1 ataataaaaa ataggagaaa aagtagaaaa gaaggtgttt gttatttggg aagtagttaa
       61 atcgagagta caagaaatga atgaacattt ctcagtgtca ttcgcagaaa atccaagtaa
      121 gtattttcga cgtcttgttt ttctgctcat cttatcattg ctgttaacag ttgcaatgtt
      181 tttattgccg gaaaaaatcc gagaagagca agtaaatagt ctcgtttatt cgtccatacc
      241 aaccagcaaa ttgaaacctt ttgattacag caaagctgat cagaaaatca aacaaagccg
      301 ggcgatatcg gtgatgttca gcaaaccgaa cgggaaaacc tatcaggaca tgcttgatgt
      361 ttttaatgat cctgagctta tggaagaatt gaatcgaccg atcttttatt atccgatcgt
      421 ttatgatgta catgaactgg aacaaaaata taacatacga acggatgaag tgacatttat
      481 tttctttgat ggaggaaaag aagcaaatcg gataactgcg ggtaagggag gaataactga
      541 ttttgataag gagttgatcc ctgaactgaa tcgacttccg ttagccaata tcaaacaatt
      601 agaagaagaa ttggatagaa cgggagtaac ttcccaaaat tccgagattg ctaattgaga
      661 cattggcgcg atgaaacggt gggtctatat agcgctattt ttaattatca gaacaagtga
      721 tgtttgatct tcaaaataac aaataaaaaa ttaaaaaata tagttgacga aattcaaaaa
      781 ttcgattaga cttaaatcaa ttcttaagga aagggtgacc tgtaatgaac ttatcaaaac
      841 gtatttcagc agaaaacaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatact gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc agcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg gccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
     9481 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
     9541 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
     9601 cggctatgat cattatttat tttctaccag ttattacaca agtatttcat caatattttg
     9661 gttaagaata cgagagtttg tgacaaaagt catgtcacaa acttttttct ttgtcatttt
     9721 ttcgtaagaa aaagcaaaat aaaagtatat acattgatta aaagtatata cttggatata
     9781 atgtacttac tatagaatga gaatggtata gagggtggcg aaatatgaaa aaaatgaaaa
     9841 agtataaaat tagaaaatct ggcaatagcg atgtaacaac tatcccacca gaagtaaaag
     9901 aattcatggg tgtgcagaca ggtgacgcaa tcagttatgt ttttcaatct gatggttctg
     9961 tacgaatgat caaagcacaa gaagaacctg atattgattc attagttgat tcgatcatga
    10021 atcaatatga agacgcgtta aaagatttgg ttgatttatg aggtatcttt cgactaaaga
    10081 aattatcaaa atcaacgcga aagttatttt acgtcactcg ccaggcgaaa tgatcggtgt
    10141 aaaagatgca aatgcattag atatggccgt caaacagcct tcacaagcag tattcaatca
    10201 agaactctat cctgaaatct atgaaaaagc ggcgatttta gcaattaatt tagctaaaaa
    10261 acatcctttt cataatggca ataaacgaac tgctcttgtt gccatgttgt tgtttcttca
    10321 attgaataat tgtccagttg ctttttctcg tcaagaagct gttgatttta tcataatgat
    10381 taccactagt tcattggatt tcgattcact aaagtccaag ataaccagtt atttacgaca
    10441 aaccgcaata aaatgaacga attaaaaact ttatttctca attagatcag catgtcatta
    10501 aggaatatgc tgattttttt atgtttaatc atacaaaata gttttttcat aaaaatatgg
    10561 aaaatatgga ttaattttga aataattgat ttaaaaggaa tacaaaagag tgaatagtag
    10621 gaaatagtgg tttagaacta gtgggctgat tttttatata ttttagttta cataatatat
    10681 attatacaaa gtagatttaa aaaatgataa acctctctcg aacggttttt tatcaaaaat
    10741 cgtcattttt ccatcaaaat ctgcttatgc aaaattataa aaaaattttg cagtttttct
    10801 tacttttttt gtcgtttaat tgaatgataa aaaccagttt ctttattcag ttaatcaaat
    10861 atgaacgaat tagatatgaa tcattgtatt tgccgttgcg gaaggatttt ttaattaacc
    10921 acagataaac attgataacg attcgcttat tgtaatcaac agattaaatt tcatatgttc
    10981 atatgtacgg atacattttc ggaccgatta attttaacca ttctgtttat ttgcttacct
    11041 attagatatc aataaaaaac tgcttaacag ctatttcagc tgttaagcag ttttagg
//
LOCUS       DAFBQH010000086        10971 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.089, whole genome shotgun sequence.
ACCESSION   DAFBQH010000086 DAFBQH010000000
VERSION     DAFBQH010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.089"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..424)
                     /locus_tag="LK694_002403"
     CDS             complement(<1..424)
                     /locus_tag="LK694_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBK5053235.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
     gene            complement(533..1564)
                     /locus_tag="LK694_002404"
     CDS             complement(533..1564)
                     /locus_tag="LK694_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK5053236.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            2288..3274
                     /locus_tag="LK694_002405"
     CDS             2288..3274
                     /locus_tag="LK694_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBK5053237.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            complement(3423..4763)
                     /locus_tag="LK694_002406"
     CDS             complement(3423..4763)
                     /locus_tag="LK694_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK5053238.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(4768..5442)
                     /locus_tag="LK694_002407"
     CDS             complement(4768..5442)
                     /locus_tag="LK694_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5053239.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            5664..6737
                     /locus_tag="LK694_002408"
     CDS             5664..6737
                     /locus_tag="LK694_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5053240.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            6743..7429
                     /locus_tag="LK694_002409"
     CDS             6743..7429
                     /locus_tag="LK694_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5053241.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            7437..8084
                     /locus_tag="LK694_002410"
     CDS             7437..8084
                     /locus_tag="LK694_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBK5053242.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(8157..9548)
                     /locus_tag="LK694_002411"
     CDS             complement(8157..9548)
                     /locus_tag="LK694_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK5053243.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIFFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            9805..>10971
                     /locus_tag="LK694_002412"
     CDS             9805..>10971
                     /locus_tag="LK694_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBK5053244.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLS"
BASE COUNT         3643 a         1887 c         2010 g         3431 t
ORIGIN      
        1 gaatctgttt tgattttgtt ccatttgaaa tttctctcac tcgctttttt gtctcatcat
       61 caaataaaat gaaagggtct tctaaacgat cgataaatac ttccattgtt gttgttggta
      121 gatgataaga agtcatgaca ggcactgcac caaggtaaga cacagaaaca gctagaaggt
      181 atgtgtcaaa agcggaactc ttataaataa taactttgtc tccggatttc actccaagat
      241 gagccaattg ataagcccgt ttcaacacac tttcgcagca ttttttgtaa gttgtttcaa
      301 gtcctaattc tgggaaagct gaaagtggct catcaaagat aatcggggtc tcggaaaagt
      361 tagctgcagc ttcttgaaaa ttcgtaaata agttcaaagg ggaatacgtt atggtctcca
      421 tcattcacac tccattatct tttgttaagt aatatacaaa ttcaagataa tcgtaacata
      481 atcctttata taataacata gtttattgtg aaaaaaagat catacatttg ttttatatgg
      541 aagaaacccg aacgaatgcc gctaattcgt gataagaaaa atcaaccatc ttttcccaga
      601 tatcttgaaa agaatgggat gagaggtatc gattaccaca taaataattt accgcgtcag
      661 catcatctaa atatagttct gcataataac gatatgtttt tctgcatagt tctggcgata
      721 cgtttttata tactgtaatt ttgctcaaaa tttggcgaat agtccgagga gaaatttgct
      781 gatcatgtga ggaacgaaaa acaaaagaat tttcgtccgt attttgtgct gttaacttaa
      841 aatagttctc cagaaatggc atcgcttctt ttggtattgg tactttattc agtctgtttt
      901 cttttctatt cagattaaga atctgcttgg tcaaagatac atctttacgt ttaagttcta
      961 ctacttcatg gatctttaat ccgcaataca gaagaatggc agtaattgca aggttcctcg
     1021 gtctattctt ttcttcaaag ctagcttctc tgcttgtttg aaagccttca atcgggataa
     1081 acaaatgctc aaaatctttg aaaagttctg catcgatata tcgcggagct gctttggttg
     1141 tttccggtaa acttgtaccc aaaagagaaa tctcttcttc aatcagtcct ttttgataca
     1201 aaaatcgtaa aaaatgattt aatgaatatt gcttccttga aatcccgtaa tagctatttt
     1261 caaacaattg ctccgtttct tggttcgctg tagtttggaa cgaacgagta tgtcgataga
     1321 ttttagctaa gaaattctga taatcttgtt tttctaaatg gataatcagc tgatcatcca
     1381 cttcttcaga aaatactttt ctcaaataat cagtaaacag atgaaaatct tttgcgtatt
     1441 catagcgact gcctaatgaa agggaagaac gattttcttc taaaaactgt tgaaagacgg
     1501 aagggagcaa gtgtgtacgt tgctttaact tttcttgcag ttgttttcta gttttgctgt
     1561 ccattacaaa ttccctccaa tagtgttcat tttctatatt ttaccgcatt tcatcaaatg
     1621 aagatagaca gtttgtcttg atcttaggaa gcaaattttt aactaagtac tagctaaaag
     1681 atatgaagta agaagataca tttttagtta ttttattagt gcagtcattc ttttgatctt
     1741 ttaattgatt tatcctcttt ttagctatgt ggttacttca tgagcaatga aatggatttg
     1801 gttcgttcta atggcaggag gctctttctt ttatttccga aaaattagaa aacatttttt
     1861 cagaatcgat tggctctcca tttattcaat aatggtttgt ctaacgatat tgttgggata
     1921 attcttaaag aagtattaga aatagttggt acttcattac tttacatgaa gatttattct
     1981 atggtaggag caattatatt gagaattaat ccttttttat ataagttgtt tgcttattga
     2041 tggattggat aaattaaacc atcacaacga gtggaaatta tatgaaattt ttcataaaaa
     2101 ttcatataga aacaaatgac attttattag aatatgactt tcattaacat aaatgttttc
     2161 aaatttcaat taaattgttt taatcaaatt ccaatctaaa tatttgtaga aaacaatcgt
     2221 ctcctataga ataaatttat aagtaacaat ataccaatca taaatctatt tgctaggagg
     2281 aatttacatg gaagagaaaa atttaacggt catcggaagc tatagtacga gggaagaagc
     2341 attatcagta attgaacggt tgcgtaacga agggtatgaa cgagatgata tcgttattta
     2401 tactactgat gaagcagcat ctagactagg atttgacgga ttgtctggta tagatgttga
     2461 aacagatgaa aatcggatgg atggtgaaga agaccgttct ttatgggaaa aaataaaaga
     2521 tactttttct tttgatacct atgattctga gacggctaca ccagaaaatg atcctttata
     2581 tcaatataga ggagatattt ctgatggaaa atttgtcatc acagtaaaag gctatcgtca
     2641 gccagaaaca acagaagata caatggatac acaagatact gttagcccag caagcacaat
     2701 ggggactgga ttagaaactg gcactggaac agatccaatg gacgtaggtg gcacgacagg
     2761 ctttcaaaat acaacggatc cattgaatat gacaccagga actgatccga tggacgtacc
     2821 tggcgagact gatacaatgg gtacaagcaa gactgaagac atcgatcgca aaccaaatct
     2881 agatgatgat acaattcaac taaaagaaga aaaattagat gtaaatacac atgatgtgac
     2941 aactggagaa gtcgacatcc acaaacatgt ggtcaacgat actgaaacgg tagaagtacc
     3001 ggtcaaacgt gaagaaatcg tcatcgaacg caaaccagta acagatcaat cttcacaggg
     3061 aacagacgaa aaattggaag atgacacgat tactatccca atcaaagaag agcaagtaga
     3121 tgtaagcaaa catacagtta ttcgtgaaga agttgggatt cataaagaag aacatgaaga
     3181 tgttgaaaaa gtgacagaag atgttagccg agaagaattg gatattgaca caagcggtga
     3241 tgtacacatt gaagaccgca ataaaaaatc gtaagcgtaa gctaaagagg ttgtgacaga
     3301 agtggtcagc ttaaagaaat ttcggcttat ttccgaagaa gctacttctg gaacaccgtt
     3361 tattcgcaac aaaaaagggc tgtgacaaga tttttgtcac agcccttttt tagggataag
     3421 cattattttt taggcttcaa cggcaattgg actttaaaat cgactccttg tggaagataa
     3481 ttggatacag aaatctgtcc atgatgctgt tcgattacct gttttgcaat tgccaagcca
     3541 agcccgtatc cgccagtctc tttcgcccga gattgatctt cccgataaaa tcgatcaaaa
     3601 atccttgctt gatcttcttc actgatagaa gggccagtat tctttacgtg aatcagccat
     3661 tctttatggt taacagatga atccaaaatg atcttatctt ctggattcgt atattttaat
     3721 gcattatcca gcaaaataac caaaacttga tggatttttt tttcatcaat tggtacttct
     3781 aatgttgcat cgttatggag aataaactct tttccatcta tgatggccat ctcttgaaat
     3841 ggtttaacaa gttgttcgat atattcttgt gtattgatca tttgctttga aagcagctgc
     3901 tgatcagaat cattccgagc gattgtcagc aaatccgaag tcaatccagt gaggcgcctc
     3961 gtctctgaca atgcttgagc gatgctttcg gattcttcaa taatagtatg attaggcttg
     4021 gtaaaaagat gttccaaact attttgaata atcgttaacg gtgtgcgcaa ttcatgagaa
     4081 gcattctcaa caaattcttt ttgtcttctc caagaaagaa taatagggcg catgcttaga
     4141 ctagacaagt aataactgat accaatagag agaatccaaa aaacaatcat acacaaaatc
     4201 ataatcgtgc gaaaagtcgc taatgaattc ttgatttgat ttgtattttc taaaatttgg
     4261 atgtaggcaa cgccatcttg attcgtctcc gcttttttcg taatcgaacg gaaagagagg
     4321 gcagatgagc cactgtcttc atttaattca atattttgga tggtattcag atttttcgta
     4381 tccattttta attctttgat ttggttgaaa cgcccaccaa tagctgcttt attcaatatc
     4441 tcaccatttt tagaccacaa gatcacttga gtgttaaaac ggtcatttga tggttccatt
     4501 aatggctgtt ccatgtttgt tctgttttgt tccgtttgag tcgggtttaa aaagggatcg
     4561 ccttgctgat atctttgtat ctctaattgt actgttcgag aatcaaaatt tattttctct
     4621 aatgacagat ctgtttctct gtatgcagat tggtttaata tctgtaaagt aatgaatccc
     4681 aaaagaaaaa agataaaagc aaatgctgct acatttgtcg caaaaaagcg actacgttgc
     4741 tttcgtgtaa tatctccatt cattgcatca caccctttgc agtatataac cgacattacg
     4801 taaggttttg atttctttat ccaatccagt acttcgcaga tgttttcgta agttgctcat
     4861 ataaacttca ataactgtaa tcgtcgtatc agaatcaaat ccccagatcc ggtcaaatat
     4921 ctgctctttt gtcaaaatga ttcccttatt ttgtaaaaaa taaacgagca attcaaactc
     4981 ttttccttgt atgggcaaaa cttgttcttt atacaagact tcattgcttt ttaaatcaca
     5041 actgatatcc ttatacttca gaacattttc atcaaagaga cctagcgagc gtttcaataa
     5101 tgctttcacg cgcatgatca gttcttctcg atagaaaggt tttgttagat aatcatctgc
     5161 acctcgttca aatccttcaa tcttatcttc caaactatct tttgcagtca aaatcaatac
     5221 tggagtctgt attttttctt tccgtatttt tgctaacact tcatatccat tcatttccgg
     5281 catcatcaaa tccaagattg caagatcata aactccactt tccaactcga acagtccttc
     5341 actaccatca taaacttgtg ttatctcgcc gattgcagcg agtatttctt ttatattatt
     5401 ggataatact ttatcgtctt ctactactaa tagtttaatc attttcccac ctcattgatt
     5461 tggtcacttt agtttatcat ggaaagcata aataaggctt aaataaattt tttatgactc
     5521 atgttaaggt tttatcgaaa gatgtcttag acaatttatt gataagaaaa aaacaacaca
     5581 agtgacaaat tgtcacttta gagttacact attcaacaag tgacattttg tcactatcaa
     5641 tctatcatag ggaggaaaaa caaatgtttc tagctatcaa agaaattcgc tattctaaat
     5701 tacgatatgg cttgatcgtc ggtattatgt tcttgattgc gtatgtcgta ttcatgttgt
     5761 caggactagc gaatggactt tcagaagagt ttaaaaaagc aatcgatgat tgggacgcac
     5821 aagagattgt tttgtcggag gatgcaaatc aagtatttgc agcttcacaa ttaacaagag
     5881 gagatctgaa atacattgaa ggaggagaga aagcaccaat cggattgtac agcggagcaa
     5941 tcaaaggaaa aaacaaagaa aatgttacag ttttcggcac aacgaaagaa gcatttttat
     6001 taccaaaact cacgaaaggg catgaattta aaacaaagaa tgaaatcatc atctctcaaa
     6061 atctggctga ttcaggctat aaaattggag atacgatcaa aattggcagt tacgacgatc
     6121 ctttgacgat tgtcggtatc tttcctgaaa cttattacac agtttctccc gtcatctatg
     6181 cgtccctaga tacgtggact gctttgaagt atggacaaca gccgtttgct tctgaagaag
     6241 aacagccgat caatgggatc gtaataaagg attcagctaa aatttcgaaa gaaaaagcat
     6301 cgaagggtct acagttatta actattggga cattcattga aagtattcct ggctattctg
     6361 cccaaaatat gaccttaaat gcgatgatat actttttgtt cttagtggtt gctgccgtgg
     6421 tcggtatttt catgtacgtg atgacacttc agaagactgc tatttttgga gtaatgaaag
     6481 cacaagggat caaaaatttc ttcatcgcta aatctttagt agcacagtcc tttattgttg
     6541 gggtggtagg cgttctgctt gctcttttgt ttgcttattt gactagtctg attttaccaa
     6601 gcgcgatgcc ttttgctgtt ttttggtcac aatggttgct atatagtggg attttgatca
     6661 ttgtcgcaat gctaggcgga ttgttttcga tacgtacgat aaccaaagta gacccaatta
     6721 cagcaatagg aggttaataa aaatgaaaaa aatattaacg atgaaaaaag ttaccaaaac
     6781 atttggttct ggtcatacaa aggtctcagc tttgaaagca attgattttt atgtaaagga
     6841 aggagaattc gtcagcatca ttggtccttc tggttcaggt aaaagtactt tccttaccat
     6901 ttcaggagga ttgcaaacac cgacttccgg agaaatagtg atcaacggtc actcactaac
     6961 tgagcttcca gaaaaaaaac gagcagattt acgctttaaa gaaataggat ttattctcca
     7021 aagctctaat ttgattcctt ttttaacggt aaaggaacaa tttgaactag ttgaccaagt
     7081 tacttcaaaa aaagaagatt caacaaaaat cgattccttg ctgcaatctt tagatatcca
     7141 gtcacttaaa aatagttatc ctaaagacct ttctggtgga gaaagacaga gagtggcaat
     7201 tgctcgcgct ttatataatg agccaagctt gatcttagca gacgaaccaa cagctagttt
     7261 ggatacagat catgcatatg aagtcgtcaa attactagtt aaagaagctc atgagaaaca
     7321 aaaagcgact attatggtta ctcatgatgt ccgtatgatt caatggagtg atcgcgttta
     7381 tcgaatggaa gatgggtact tgacagaaga agagaaaaag aaggaatgaa tccttcatga
     7441 caatgaaggg ctatccacgt ataatcagag ataaaggaga gatgtggatg ccaaaagaaa
     7501 cttttttcaa tttatcgaac gcaaagaaac agagaatcga tgacctatta ctggagatat
     7561 tttgctcaca gcacatcagc caagtcaaag tctcagaaat cgttgacaag atggggatgt
     7621 cgcgcggtgc tttttataaa tattttcaag atttggaaga tgcttatttg tatacgatca
     7681 aaagcagttc tcacatagtc catcaagata tcttaaaatt cattagcgaa aacaaacaac
     7741 atttcttttt ggggatcgaa aaatatttag agtggtgctg cgacttagat cacgaaagta
     7801 tctattggca aaggttatac ttgttaaccc agcgaaatga tcagcagaca accaaacgta
     7861 gagaactatc ggctgattct ccaatgaacg aaaaatggct ggaattatta agaatcaatc
     7921 atttctccat tcaatcgtcg gaagaagcag ttagtttcct atacttttcc atgaccctcg
     7981 taatgaacac tttgacggat ctcattgcaa ataattggac aaaagaagag ttgttaaaag
     8041 actttcgcta taaatctcgt tggattgctg aaggaataca ataaaaagaa acagctatgt
     8101 gtatttgtcc tgaaattttt atcagggtgg tttatacata gctgtttttt gattattcat
     8161 aactcgaata agcggatgag ttcttcttca tttgcgaatg acacactatc atcaatcaat
     8221 atatgaggaa catgggcatt caaaatgggt tgattcgtaa cgatcagatc atactcgaga
     8281 agattgtatt tcgacagctg atcttcagta atagcagttg tctcagagtg aattttatgc
     8341 acagcaagaa tatcactgat atattcttca atatattgac tatgtcgaag acttaagtcg
     8401 ctgataatca gtattttttt atcagaagta taatggtgaa tttcaggatt accgataagc
     8461 atccaaaaga tatagctaga taaataataa atcagattct gattcagtag agtggaataa
     8521 aattgaagtt ctgcacgaaa tccagcatat agacgtatct ggcggtgttg catctgctgt
     8581 gcaaagtaag ttgcacggat cgtcaaatga gtcgtatcgt atgggaaaat acgggagaaa
     8641 aaaaaaattt gcgttaataa ttgaacaaca tattcttcgt ttttttcttt gcaatcagcg
     8701 gatgaaaacg aacagtagga catatttttt cgtatctgct cactgatttc atttttttct
     8761 ttctccgaaa attctgaaaa aatcgtattg acaaaatagt tcactacttt tatatagctg
     8821 ctgtacttca attttcctaa gagatttttc aagattaaga agtctttttt atcgatattt
     8881 atcaactgta cataagagtc tccagctttc cattcaaatg gagttccttg ggattctctc
     8941 atgagatgca caacaaaata cataataaag aaagattgct catatgggct ttcttcctga
     9001 aacaaattca tctgacaatt attcttgacc ataatttctt tgatcttctt tttttcgatt
     9061 ggaaatggca acgtgcgtct tccgtagtaa cttaaaaaga aaaaggtaac aaagatacgc
     9121 aactggagct cacttttaag gctagttaac tgaaaacctt ggtcaacgat cacttttaat
     9181 aaatagcttt ctaaaatttg attgcattgt ttcaacttac gtgtaaaaga agaccggctg
     9241 atggaaattt tttcacaata atactcaatc gttttacccg gattgacaat aatctcattt
     9301 aataaacgac tgtttgtaga actttgcgac atttccatca gaacatttaa ataagctggt
     9361 acattcaaat gttcaagacg ataccccaat ttttgtgaag attgaatcgt tacatccgtt
     9421 ccccagcgga ttttgatctc ttcgatatac cttacaaatg tagattctga actaccaatt
     9481 cgtttggcaa gttctttttt agtcatccac ttgtcagaaa tggacagaat tcgaatgatt
     9541 ttttccatct ttctatagtt tcgttccaac aacctgatca tatgtttttc tccctcaacc
     9601 cttttctttc aatgaaacgc tcattaatcg aaggaatcaa cttcataaat gaaaccaatt
     9661 ggtatgaaaa ttggtgtgat tccctttagc gcccttcgta taataattat catattaaaa
     9721 taaataaaat tatgtaacgc ctgtttattt taataatttt cactattcac gtcaaaacag
     9781 aaaaaaagaa aaaggagggg aaaaatgacg accacaggga agaaactgaa agttattttc
     9841 atgctgataa tattgagttt atcaaacttt gtgccattat ctgcaatagc agacactaca
     9901 gatgatccaa cagttttaga aacaatttca gctgaagtca tttcggatca gtctggaaaa
     9961 aaagcactga acatcaagct aaatgcgaat aacaccagtg ctgaaaagat agaaaaagaa
    10021 attggtctag tcgaaaatta cttaagtgat gtggaaagaa aagaaggaga tggctatgct
    10081 tatcaggtaa atagcgggaa aattacgttg gaaatctcat caaacactaa acaaactatc
    10141 gatctgagtt ttccaatcga tccagcactt taccacagcc aggcaaacaa gctgatcgtc
    10201 gataataaag aatatgacat tattgatgag acagaaaata agaaagatac agatgtgtca
    10261 gtaccaaagc cagacgaaat agaagaagaa tcatcaaaag aaaacgaaaa ttctgtcagc
    10321 ccatttacat tgcctacatt atccttgcca gctgtgagtg tgccatctaa tcaaacgatt
    10381 cctacagaat atacaacaga tgatcagggc acttatccta aagccagttg gcaacctaca
    10441 ggaaatacaa atgttcttga tcatcaaggc aataaaaacg gaacaaatca atgggatggt
    10501 ataaattctt ggaatggaga tcctaatgat cggacccatt cgtatatcga atatggagga
    10561 accggtaatc aagcagacta tgcgatacga aagtatgcaa aggaaacaag tacacccgga
    10621 ttgtttgatg tttatttgaa tgctcgtgga aatgtacaaa aagatatcac gcctcttgat
    10681 ctcgtattgg tcgtagactg gtcaggaagt atgaacgaca ataatcggat cggtgaagta
    10741 aagattggtg tcgatcgttt tgtcgatact ttagcagata gcggtatcac agacaaaatc
    10801 aatatgggat atgtcggcta ctcaagcgaa ggatatagct acagtaacgg tgcagtacag
    10861 atgggttcat ttgattcagt gaaaaatcaa gtaaaatcca ttacaccttc acggacaaat
    10921 ggtggtactt ttacacaaaa agcactaaga gatgcaggaa gcatgctatc c
//
LOCUS       DAFBQH010000087        10957 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.090, whole genome shotgun sequence.
ACCESSION   DAFBQH010000087 DAFBQH010000000
VERSION     DAFBQH010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.090"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(362..1480)
                     /locus_tag="LK694_002413"
     CDS             complement(362..1480)
                     /locus_tag="LK694_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053245.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1951..3258
                     /locus_tag="LK694_002414"
     CDS             1951..3258
                     /locus_tag="LK694_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBK5053246.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3377..4576
                     /locus_tag="LK694_002415"
     CDS             3377..4576
                     /locus_tag="LK694_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBK5053247.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4603..5871)
                     /locus_tag="LK694_002416"
     CDS             complement(4603..5871)
                     /locus_tag="LK694_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBK5053248.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6034..6680)
                     /locus_tag="LK694_002417"
                     /pseudo
     CDS             complement(6034..6680)
                     /locus_tag="LK694_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7088..7591)
                     /locus_tag="LK694_002418"
     CDS             complement(7088..7591)
                     /locus_tag="LK694_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK5053249.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSPSMRKRWQRY
                     FGKESSI"
     gene            complement(7714..8478)
                     /locus_tag="LK694_002419"
     CDS             complement(7714..8478)
                     /locus_tag="LK694_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBK5053250.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8586..8861
                     /locus_tag="LK694_002420"
     CDS             8586..8861
                     /locus_tag="LK694_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBK5053251.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9299..10249
                     /gene="yidC"
                     /locus_tag="LK694_002421"
     CDS             9299..10249
                     /gene="yidC"
                     /locus_tag="LK694_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBK5053252.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10428..10628)
                     /locus_tag="LK694_002422"
     CDS             complement(10428..10628)
                     /locus_tag="LK694_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053253.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT         3292 a         2310 c         1959 g         3396 t
ORIGIN      
        1 aacggtgttc cagaagtagc ttcttcggaa ataagccgaa atttcacaaa aatttgagaa
       61 ataatttttg tcaatctctt cttatttctt gaagctgacc acttctgtca caacctcttc
      121 cttaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaccaaaca aaaaatatga
      181 agagctattt ttgcttgctg ttcttattac ttgatgcaga acagcttttt cacaacctct
      241 catacacggt gtttaatcag ctgaccttcg gtattagctc aaaaaacgga aaaatatgtg
      301 aaactatttt tccgtttgtt tctctaatac tcaggtcaaa acactgattt cacaacctct
      361 tttaataacg aagaatcgct gtcagactct tttcatctgg cgcatctttt tgatccaaaa
      421 taaacacttt cccgccatta atgatcacat catctgctaa tgcattcaac acttgacgcc
      481 gatcatattc tgtttcagga tcttcaccga aaccatcagc taagtttgac gtagcgataa
      541 ataaatgtga cacttttcct tctttcgcag ctggaacgat atcaactaac tgatcgatga
      601 atttcttatc taagagttta ttgtattctt ttgattcttg agctgcgagt tcacttgaca
      661 gcttcttcgc cccttcttcg atatctctaa aggatagctg cgcaggagaa gcagaaatcg
      721 caacgtctgc acaataatag ggatttttag caattttttt gaacatcgtc tgattttctg
      781 gtaatgcaaa taaataaatc ggccattgtt caggattttc gaaagaatcc ttcaagtacg
      841 tatcaacagc ttggtaatag ttgacccaat cgatctctac ttcttcatct tttggattta
      901 cgccatggaa agttactcca tctcgggctc cgcctcctcc attagaagag aagttcaaac
      961 cgccacctgt cagttcagat cccaatgcag taacgacatc aaccggtgcc ccttctggta
     1021 gagatacttc ttttacttgt ttgttgctga cctcatacaa tttcatggag tcgcggttca
     1081 atcccaataa ataataagaa tagttgaact gcccattttt gataatagcc agtaaataag
     1141 gaagatcact tacatagtat tgatcgtcta ccgggatact caaacgatgg atataggtct
     1201 ggtcagcagt tataatgacc gctacactcg tagttgcact acgccaaaac gaaggatctc
     1261 ctaacaaatt ttcaattttt tcttggaacg gggaccagtc atgttcggaa tattttttct
     1321 caaaacgttt ctttgcttct ttcgcaaaat ttttgaattt gatctgatct ttttccacat
     1381 cttgatgggc gacatgcgta ttcagcatga tcgtgacgaa tggtccttta gcgccttctg
     1441 aagtcaattg tgtcaataat tctttatcct tttttgccat aaacgtaccc ctcctaaaaa
     1501 aatcattcct ttgtttctat ctaaagtcta ccacaacact atttttccat acaataaaac
     1561 gtatctaaaa aaataaaacg gtttctacgg ttttaaattt agctattttt ttaagttatc
     1621 ttcataattt cctcaattat ttagccccag tcttcaacta ttcttcatga aatgctgata
     1681 aactaagaaa aagaataaaa gaggtggatc ttatgcattt gattatgatt ttcagtttat
     1741 taagtattat cagtttattt gctttcagcg cttcccgctt attagatgcg gaaacacagc
     1801 ctattcctgt aaaagtacgt aaaaacgacc gctaaacttc cataacgcgc gttcttatcc
     1861 taaagttttt tcagaaacat tgaaactctc tcccctctct gataaaattt taatttgtag
     1921 cgaaacaaat taattacaag gagatcggat atgtccataa aaaaaacacg tctagtccta
     1981 ttattggcta gtctcattct ttgccttgct tcttttctac cagctgttgc tgtgcaagct
     2041 gaagatacgt tcaaagtaaa tgcaaaagca gcttttgctg tagatgcgca aagcggtaag
     2101 attctttatg atcaagatgg agaaaaaacc atggggatcg cttcaatcac aaaaatcatc
     2161 ggtctttaca tcgtccttga tcaagtaaaa gaaggaaaac tgtcatgggg tgataaagtt
     2221 tcgatttcag attatgcaga aaatttgagt atcaccccag atttatcgaa tgttcctttg
     2281 cataaagaaa acacctacac tgtcaaagaa ttattcgact ctgcaatcat ccagtcagcc
     2341 aatgcttcaa tggtggcctt ggcagaaaaa atctctggca gcgaagctaa attcactgaa
     2401 cgaatgaaag aacaattgaa agattgggga atcaaagatg caacaatcgt taatgcatca
     2461 ggattgaaca actcttatct tggcgaaaac cgaccagaag gcactggaga aaacgacgaa
     2521 aatcaaatgt ctgctcaaga tgtggccatc gttgcccgcc atttgatctt ggatttccca
     2581 gaaatactgg atgtatcaag tactacgacc caaatgtttg gcgaaaatac gcaatcacca
     2641 gtggaaatgg tcaactggaa ctggatgctc ccaggattcg ttaattacaa ggaaggcgtg
     2701 gacggattaa aaactggtac gactgagctt gcaggtgctt gtttcgttgg tacgatcact
     2761 aaaaacgatc aacggatcat cactgtcgta ttgaatgcgg acggccatgc ggaaaacccg
     2821 agtgcccgct tcaatgaaac agaccgtttg atggactact gctatgacaa ttggtcagaa
     2881 aaagagctcg gaaaagctaa cgcaagtatt cctagtctaa aaaccattga tgtaaaagac
     2941 ggaaaagaat caacagttcc tgttgtgctt aaaagccctg taaaagtatg ggtacgaaac
     3001 gatatggata ctggcaaatt aacgatcacg ccaacacttg acaagaagca agtcactgac
     3061 aatgagatcc aagcacctat ggataaaggt acaaaaatcg gtaccgctac gatcaccttg
     3121 gcagacgata aattaggcta tttagaagac aacaatgcac catcaacaga gatcattacg
     3181 gataaaacag tggaaaaagc gaatatattc gtgattggct ggcgacgcgt cgcagaattt
     3241 ttcggcaact tgttttaatt gaacaataag aaaaacatct ttccatcgga aagatgtttt
     3301 tttgcttttt ctagtttaaa taacagcttt tttcatctat tttcgttata ctgtataaga
     3361 aaaaggagtt gaatttatga atctaaccag agatctgatc tttttagttc tctatcttat
     3421 tggggtattc tctatcgttt attggcgaaa acagaagaag gatccttcct tgttctttta
     3481 cggcagtacg tggacattta cggtaatctt aacttatttt gtcgtgttgg aaatttttta
     3541 ttcgaccttt tattttttga ttccattgtt gttctttagt atctttgctt tttcttattt
     3601 cacagaaaaa cgaaggctac tgaatggtct tttattcaat gttttactca tcagtttcgg
     3661 catctacttg tttgtcttgc tgtatgaaac gcaaaatatt tttcttggcg gactgatcgc
     3721 cttgatcacg attccattgc tacttgtcct tttatttggt atttatggat tgattgtttt
     3781 tcttttttgg aacggggtca cagttttacg tagagaatcg cattcattag ctaatctttt
     3841 aacgttgata ctagccattt ttcttacact gtttcttgtt tttgactttt tcttgttaaa
     3901 atatttgccg caatggataa atgctttgtt tttctgcgtt cccctgatct tgatctatct
     3961 atttatcgta ttctacaact ttttgactgt ctcattcttg tatcagttca accggcctcg
     4021 atacaatcaa gacttcatcg ttgtgttagg cgctggattg atcaatggag aaacagtttc
     4081 tcctcttcta gccaagcgaa tcaataaggc aattgctttt tatcgggctc aaagccgtgc
     4141 tacattaaat ccgcccatct tattgatgtc tggcgggcaa ggagcagacg aaaaagtacc
     4201 agaggccatt gcgatgaaac aatatgccat ggaacaagga atccctgaaa gagacattct
     4261 tgtcgaaacc aattcgacaa ccactttgga aaacatgctc tattcaaagg aaatcatgga
     4321 tcagcaaatg aaagggccat accgcgcgat ctttagttcc aacaattacc acatttttag
     4381 agccggtctt tatgcgcggc aagccaaatt gaaagcaaat ggtattggtg caaaaacagc
     4441 tttctactac ttgcctaatg cgtttttaag agaatacctc gccatcttga tgatgaacaa
     4501 aaaaagacat atgatcattt gcggattgct tttcgcaggc tccgcattct tatcgctgat
     4561 tactttactt ttctaaaaaa acaaggtgtg acaaaacata tgtcacacct tgtttttttc
     4621 tttcattctc ttaactattg gcaaaaagag tatccctttt ttgaccgcta cttgctgagc
     4681 agaataaata ccgctgttgc ctgaacacat aaagctgatc aagcagatca taaagaagta
     4741 tactgccgct tgtgaaccga acaattcgat ccccatcaca aagcaggcaa tcggtgtatt
     4801 tgtcgcacca gaaaatactc cgataaagcc caatcctgca agaaaaggaa cagaaacatg
     4861 gagcaaaggc gctagtgctg caccaagcgt tgcaccaatt tcaaacaatg gtgtcacttc
     4921 tcccccttga tatcccgcgc ctaaagacaa aactgtgaag aataacttcc caataaaatc
     4981 atagacatgt gcctgtccat taaatgattc ttgaagtaac ggaagactca atccgagata
     5041 acgttgtgtt tgcaaaatca atgctgctgc cacaactaca gctccaccta tcgtatttcg
     5101 acaaacagga tgaggaatcc attgtgcata tcgcgctttt aaaaagacga tcgaacggct
     5161 gaatacccaa ccaatcaaac caaaagcaat cccagcaaca aaaactttga agaacagcaa
     5221 catactccat tcaggaatca cacccatttg gtaatgtaga tgagaaacac catagctttc
     5281 tgtcatgaaa tttgcaaaca gtgcagcaaa gaagctaggg aagatcgctt ctgttcggac
     5341 tttcccgatt gccaacactt ctaatccaaa taatgttcca gctaaaggtg tgccaaatac
     5401 agaactaaaa cccgcactga ttccactgat aatcaagatt tcccgttctg atttatccaa
     5461 tcggaacaaa cgtgccgtat tatctgctaa cgctcccccc atttggactg ctgttccttc
     5521 acggccaaca gatcccccaa ataaatgggt agttatggtt ccaaacagtg taagtggaat
     5581 aagccgcaac gggatttttt cttctccacc atttccttga tcgatcacta agttgtttcc
     5641 acgactagaa agtccgccgt gatacgcata gagataagca aaaagcgcac cactaactgg
     5701 tagtagacaa aggagccaag gatgtgatag tcggatatct gtcacaactg tcaggctatt
     5761 taagaaaaaa gcagacaagc tccccatgaa tataccgaga atcgctgtga tcaccagcca
     5821 cttcagcata tacactaaaa tgcgtactgg actcggtaat tgtttttcca taaaaaattc
     5881 ctcctcaata aaaaaacgcc tactaaagaa aggggctgtc cccagacttt agtaggcgtc
     5941 attagctatc tttcgttagc atttaaaggc gaacaccatc acctattcac ttccattaaa
     6001 agatagcata tttttctgta aaaaacaata aaattatcgt tctttcttta cacgttcagc
     6061 aaattcatag aaaggagcca atttcgttaa ctgttgatta cattggtcac tatcatgaca
     6121 aatataattc ccctttttcg tatacgtgcc atcagaacct gctttcgttg tagaaagaaa
     6181 aagcgaaaca ttcgatgttt tctgacagat tgcacagatt cccttattta tcgttggtga
     6241 taaagtaccg ctaatccctt ttaatttttc atcttcgtaa tagaccatga attttttctg
     6301 cgtacctgga tcattccagc cgatgtacgt atgttcttgc aagtcgattt cattccaagc
     6361 tggttgtttg agttttttca cttttcggaa taatttctcg atttgtggtt tgctaggctg
     6421 cataaacggc tgaacgattt gcttcagctc ctccaatgct ctttctgctt gcgcttttgt
     6481 cagttttacg tctaaaactg tcgttaaaaa tgtatcgatt ctggcagttc gtgatctaat
     6541 atgtctcgaa tcttttcagc tgctagtgcc tgaactgttt gtaccgtttt tggatcattg
     6601 acagatgagt acgcatttaa taactgttct acttctctta ttataaaaaa atattggtat
     6661 ggttttattg tttgttccat gattttaaag tcctttcttc taaccaaagt gcatactttg
     6721 tagaaaaaag tgatcttctg caaagtatgt gttacctgaa gatcccggac tcttttaaat
     6781 aagatacgtt ttgcatctta tcatcacccc ctttaatgct tattattcta tgtacatgat
     6841 tcattcgata aataaaagta aaccgtatct tgaacagata gtcaactgca aacagaaaaa
     6901 aggactgtga caaaagtcgt gtcaaagtcc cttgtttcga atgaatggtg ttcaaaagct
     6961 ggcatctgcg gtaataagcc gaaacttcac aaaaatttga gaagcaattt tcgtaaattc
     7021 cttcttattt cttgaagctg accacttctg tcacaacctc aatatggtct attattggtt
     7081 ttatctatta aatcgaggat tctttcccaa agtaacgttg ccatctctta cgcatacttg
     7141 gtgacatagg ctgagcggct tctttgactg cgcagtattt gtctacgaat gttacgatat
     7201 atccttcttt gtacttaggc ggtgcaatcg ttgcgcccca catgtgcttc aaaatgatat
     7261 ctcgctcaag atccgacaat tcggtgatct tttctgcatt ctttacagca attcttggat
     7321 ggatatacgc atgtgtgcct tcatcaaatt tcgtcgtacg ccaatcatag tagaacagat
     7381 catgcaaaag tccagcacgc gctgttgctt tagcattgcc accccatttt tttgcgagca
     7441 gataactata gtaagaaaca ctgatggaat gctccaaacg agtggaatga acatgctgtg
     7501 tgtaattcgc taattttttc actgcttctg tttctaaaag atcttctaca taagacatat
     7561 attcttcgtc ttcacgccat ttttctgtca taattatcga tctgccacct ttttgaattt
     7621 cattttcttc tgaaattgat ctaagtatac caccagtgtt cggattattc tagtcgcctt
     7681 ggataaaata gccaaagctt aatagttcct taatcataaa gaaaaatgat aaagaacaat
     7741 ccctgccgca accccaacat tcaacgattc cgcatttcct ttcatcggga tatatacgtt
     7801 ctgatcagtc aaagacaaga tttcctgact taccccctga ccttcgttcc ccatgattat
     7861 tgcgaagtta tctgattttt ctactttgtc taatgcgtgt gcttcttcat ttaattctgt
     7921 gccatacact gtaaaccctt tgtctttcat taaaggaatc acttctgtca aatctttttg
     7981 caacacaggc aaatgataat tgcttccctg catgctgcgc agcacctttg tactataaat
     8041 atctgccgtt cctttgccta ataaaacacc agccatatca aatgcatctg ccgtacggat
     8101 catcgtccct acatttccag gatcttgtac attatctaac aacagccatc ctcctgaaaa
     8161 atctggcgtg ccttcagccg ttaacgccat cactgctaag attccttgtg gtgtaggtag
     8221 atccgataca gcatccatga cctcatccga aactagaaaa gtagaaatct ctttgtttgc
     8281 ggccagccag tctccccatt ctttcatgcc tcgctgtgtc acaaaaagtt cttttatttc
     8341 tgcatcagac gacgcagctt cttcaatcag atggaaccct tccaaaagat attcttcctt
     8401 ttcttcccga tatttctttt tatgtagttt ttttaattct ttgattcttg gattttttgc
     8461 tgattggatc tctttcacat tcctcacctc cccatgatta tatcatggaa taggtgcttg
     8521 agcttgtttc atcctaaaaa aatcggtatc attaatttat aaaataggaa agaaaggtga
     8581 cgaacatgcg caaagttaaa atgaacgtcc aaggacgcgt ccaaggcgta ggatttcgct
     8641 acatgaccaa gatggtcgct gatcaactag gcgtgactgg aacggtaaaa aacgaagatg
     8701 acggctcagt cacgatcgaa gcaatcggca atgacgacat catccagaaa tttattgaag
     8761 aagtcaagaa atctccgagc ccaagtggac gagtgcagta tgtggatgtc caagaagacc
     8821 cgatgattga ggaacgaaag aaatttgatg tggtgggata aggttgtgaa aaagctggtc
     8881 tgcatcaagc attaaggaca ggttgtgaaa aatagctctc catatttttc aacaggctgg
     8941 acttaatgcc gtagatgcaa gcttttgaac accgtcgata aggttattct tgaggcgctt
     9001 tgcctcaaaa attaaggaca gcagtgaaaa agctgatcta tcacttgcag tgagttcatt
     9061 tgactccgct ttttcgggca gaaaaaaagt ttttagttta ttaaagtaag aaagaaacac
     9121 ttggaatcat tctacttttt cctagtattt ctttcttgat tttttgtctt tttttcaaaa
     9181 tttttcgcta cgtaaaagaa cgaatattta tatgaaattt ctatgaagtt gattgaattc
     9241 catgcgcttt tttagtatag ttaagtttgt gtaaaaatta tagaagagga agtattctat
     9301 gaataaatgg aaaaactggt tattgggatc cggacttgtc agtatcctgc tctttttatc
     9361 tggctgtgtg cggatggatt caaacggaaa ccctgacact tcgggaatcg tttatcgagt
     9421 actcgtccat cctatgggcc aagcgattac atatctagtt gaaaatttca attggtctta
     9481 tggctgggca gttattgcaa tgactgtgat cgtccgtatc atcatcttgc cactaggtat
     9541 tagtcaatcg aaaaaaacaa tgatccaatc tgaaaaaatg caagcgttaa aaccacaagt
     9601 tgaagcagcc caacaaaaat tgaaagctgc aacaacacgt gaagaacaaa tggcagcaca
     9661 agcagaaatg cagcaagtat accgcgaaaa cggattgagt atgaccggcg gaatcggctg
     9721 tctgcctcta ttgatccaaa tgccgatctt ctctgccttg tactttacgg cacgttatac
     9781 agaaggtatc cgggaatcat ctttctacgg catcgattta ggctcaccaa gtatggtact
     9841 tgttgccatt gcaggggtcg cttatctgct tcaaggatac atctcaacga tcggtattcc
     9901 tgaagaacag aaaaagacga tgcgtacgat gctgatcgtc tctcctgcta tgatcgtctt
     9961 catgtcgatc agtgcgccag ccggtgttac actttactgg gtagtcggtg gtgtgttcag
    10021 ctgtctgcaa acattcatca caaacgttat gatgaaacca cgtttgaaag cacaagtggc
    10081 agaagagttg aaacaaaacc caccaaaaca agtcgtcact ccaagaattg atgtgacaga
    10141 atcagctaaa ccagctgaca gcaaacaagc aaccaaacaa atcaagcaac ccaaaaacag
    10201 tacaaacaat aaaggccgca acgcaggaaa gcagcagaag cggaagtaga tagtaaataa
    10261 gagtgtaata aaaattcatt aacagtttta gcaattaaaa atatcgacca agatttttga
    10321 aaaagaaatc tggtcgatat ttttatttaa gtgaatgttt aatttgaatc atcgattgat
    10381 cgctatctaa ttgcagcagc atctttttat gaagaaacaa ctttttctta tctatcaacg
    10441 tagacaggtg ttgctgtttc ttctaatagt gtacttaatt ccttaatatt ttgttttcct
    10501 tcttctaaca tccaactact cgctttttct tcgccttcgg taacaaaagg cgccactccc
    10561 ttcgcccatg ttgctcgccc acataagaca ccattctcca taaaggacaa cgcggagata
    10621 ccacccatct aaataacatc cataaacctt tctagtagaa gaatagtgat agtatccatc
    10681 cctttttcta ctgggaatac aattacacga ttcaaagctt gatacacaac atttcccttc
    10741 ttataaaccg ctattgtaac cgaattgttt tgaatcacct ttttgggata gaaactggct
    10801 cctttccgca acttcttaaa tgttggtttc ttttatactt tctttgtagc aactacaaat
    10861 ttcttcaaat ctctaaatgt ttgcataaca accgcctttc tgacttttac tcaggatggt
    10921 atttaaaaat cagcaaaata aggcttctct ccatctt
//
LOCUS       DAFBQH010000088        10729 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.091, whole genome shotgun sequence.
ACCESSION   DAFBQH010000088 DAFBQH010000000
VERSION     DAFBQH010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.091"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..537)
                     /locus_tag="LK694_002423"
     CDS             complement(<1..537)
                     /locus_tag="LK694_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK5053254.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHFVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFKRNISERKKDYVVFEEEF
                     GHLEGDTIVGIHHKSVVIT"
     gene            complement(619..969)
                     /locus_tag="LK694_002424"
     CDS             complement(619..969)
                     /locus_tag="LK694_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HBK5053255.1"
                     /translation="MINNYLHYKAINWNVIEDELDNVVWERATSLFWLDTRVPIENDR
                     SKWANLQLQEQEQLNRLLILLTNIATYQSNELGEIIRDSARSQQEIAIINNFQFTEMV
                     HTKAYNRILSIRQF"
     gene            complement(1018..1461)
                     /gene="nrdI"
                     /locus_tag="LK694_002425"
     CDS             complement(1018..1461)
                     /gene="nrdI"
                     /locus_tag="LK694_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBK5053256.1"
                     /translation="MQIIKVYYISLSGNTTSFLADLDTYCQKQYQKKVDYVNVKDLVK
                     LGKPLTFDINESYFVFLPTYLEGGNGIETGDVEILTTPLRQVITYNKNRDYCLGIIGS
                     GNRNFNKQFCLTAYQYADDFGFTVLDEFELKGTTEDIERIAETMQ"
     gene            1608..2273
                     /locus_tag="LK694_002426"
     CDS             1608..2273
                     /locus_tag="LK694_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBK5053257.1"
                     /translation="MKNSGCLCTVPLFASLSLDEKNEIKCLLHHQTFKKGEIIFSPSS
                     EEQLLIISLGAMKIYRISRTGKEQLIRIVKEGDYDGENYLFGLPNDCLYGEAVEETKT
                     CTLYKSDFNKVMARYPQLSYRLLQLNACKTVAVEKQLQLLAFDRIEDRLALYLNQLSI
                     AAKMGEPITLPISMRELANYLATSPETISRKLRDFQERQLITRKGKQITLLEEFWFIF
                     NYI"
     gene            2263..2718
                     /locus_tag="LK694_002427"
     CDS             2263..2718
                     /locus_tag="LK694_002427"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010905674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBK5053258.1"
                     /translation="MIYKEEDIKIGIVIARFNDFLTKSLLSGCEDGLTRSGVLTENID
                     VAWVPGAYEIPLTAATMAETGKYDAIITLGAVIRGATSHFDVVVNQASSGVSQVGLTH
                     KLPVIFGILTVENIEQAIERSGTKAGNLGFTYATNTIEMINLFKEIKQQ"
     gene            2868..4247
                     /locus_tag="LK694_002428"
     CDS             2868..4247
                     /locus_tag="LK694_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HBK5053259.1"
                     /translation="MTIQFKQTLAPTKDNSGKPIILSDATFAQRNEKVLQLMREYDFS
                     SLMIYADKEHGSNFEYLTGFIPRFEEAIQVINQDGTSTLILGNENYNKTSMARTQSEG
                     ILCPFFSLPNQPMDFTKTFADYLKDVKVDASKRIGLVDWKLLSNDMDNFHNHHSIPAF
                     IVDGIHEVFGSDKVVNATQLYMHPKEGARVTNNAEEIARYEYGASLASDAVLEAMNQL
                     TVGQSELAAGNHLNRDGQYPSVVTIAAFGDRFEGANIYPTDNTLNDGDKVALTVAYKG
                     GLSSRSGYAVKEESQLAEHDPGYLEEVVYPYFQAYKWWLDNLTIGRKGGDFYQAFSDF
                     YPQATYGWNLCPGHLVADEEWLSSPFYQDSDATIQSGYIFQLDFIPSQAGHNGVSAES
                     TIAVADAALREQIKAEQPELWARICNRKDYLKEALGLELSEHLLPLASTFAYYRPFLL
                     DKKTAIYFG"
     gene            4384..5226
                     /locus_tag="LK694_002429"
     CDS             4384..5226
                     /locus_tag="LK694_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBK5053260.1"
                     /translation="MPLDEQIELLFTIDEGYLNPLKVALTSIRQNNPGQAFRIWLIHE
                     SIAMHTIRELQKLTDYLQFGFEAIKIDGSRWNSAKTEDRYPKEMYFRLLAGEILPKEM
                     KRVIYLDPDILVVNPLLDLWQTDLEGYMLAAATHVGLTDVSTRVNQMRLDVDHAYYNS
                     GVMVIDLDKAREIIKWSDIAQMIEKYNLLLILPDQDILNRLYGKYTKEIPEEIWNYDT
                     RKYMRYFTKSLAQHDIHWVMAHTAILHFCGGPKPWDDKHDNRFTSLYLTYQNQLKRIE
                     DLMK"
     gene            5300..6748
                     /locus_tag="LK694_002430"
                     /pseudo
     CDS             5300..6748
                     /locus_tag="LK694_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            6863..8635
                     /locus_tag="LK694_002431"
     CDS             6863..8635
                     /locus_tag="LK694_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK5053261.1"
                     /translation="MNNNETKKTYELRITADEIKSTDPTFTMKNLSLGDNYQPKDAYF
                     RTAEGEITRRNYGKNERKISNQTLPRLAIQMFEQSIVSLPESERATFPVCRYHSGDEI
                     IRGIFPDVDSFKKKRKSIEFLIYKYNEDKQIVIYCWSIFSTLLFVQECLKRFGTSGDE
                     FILVYKGKEKKEIRVGETQTMTNHLLKSYVLHLLKSKNLILRGAPGTGKTFLANEMAA
                     DVVSNGRTTQINELSEDEKSRIGFVQFHPSYDYTDFVEGLRPTTSDDGVVSFTLKDGS
                     FKSFVEKAKKTKKVNGQDNFEEAWAKFFDAVTEASIEGEGYNKLKTLTGKPVRNLLAY
                     ERNEIQGVYPAKTNTYLNHDQIYNVYRGLPGTPKGGFDAYRKAIVKHLKEEFGLKNYT
                     ASTVDSTNNQNYVFIIDEINRGEISKIFGELFFSIDPGYRGHKEGVYTQYANLHENPE
                     EKFYIPNNVYIIGTMNDIDRSVDTFDFAMRRRFTFLEIKAEDSAQNMLTQEKTKELMT
                     RLNEAIVAKEKGGLSTDYQIGASYFLSIDNGEETMEQLWDTKLYPLLKDYFHGEHKAK
                     EKLEILEKVYSNDGDLDEAETSTR"
     gene            8610..9902
                     /locus_tag="LK694_002432"
     CDS             8610..9902
                     /locus_tag="LK694_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine 5'-monophosphate oxidoreductase"
                     /protein_id="HBK5053262.1"
                     /translation="MKLKPLHDNQEINDSVVQHPIITNLVNRDLSSLEKENFIIFPQQ
                     LNQSKDLSEDNLIFQSRNGKIWTCNVVGILSDSTDELRIHSRFANQMKHEDFFLRYML
                     QRVLNYNVINDHFSASKKMTYYDLLVFLFPYYLNEAMRKGTYKEYVKREYNNANIKGA
                     VDVAKHIRSNVPFVGKVAYRTREFSYNNHLTQLIRHTIEKIQNEYDFLLSGDEDTKEN
                     VVLIKQTTPGYARLNQFNILQENILHPVKHSYYEEYSALQQICIQILSEEKSGFGSDK
                     NQIHGIIIDVSWLWEEYIGKVTGWKHYGRDKGLATMHLFQEPNRSPRYPDFTFNNIPI
                     DTKYKKHLDTRNDYNQLVTYIHIMNLDQADTMKGGFLQPTSDPLPANHGYKKIGILSG
                     LGGEIFTYHFFIPQAASSYLEFVEQMREVEAALQTTFQ"
     gene            complement(10365..>10729)
                     /locus_tag="LK694_002433"
     CDS             complement(10365..>10729)
                     /locus_tag="LK694_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5053263.1"
                     /translation="DVKVLETLSEEANLPNILGDKGYISHEIHEKLALKGITISVPPR
                     KNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVL
                     LSRAQRRFEGTLRYSLGY"
BASE COUNT         3673 a         1767 c         2020 g         3269 t
ORIGIN      
        1 tgtgatgacg acacttttat ggtggatgcc gacaatcgta tcaccctcta aatgtccaaa
       61 ttcttcttcg aaaacgacat aatctttttt acgttcagaa atatttcttt tgaatgcctg
      121 ttttcctctt ttttctttat gtccgtttgg ttttcttttc cctttcattg gtaaatcgtg
      181 agggtcaaag gttttatctt taaacatccg atatagtgta cgtaccgaac aagcaatagg
      241 aaactctgct cgaccaataa ggacatctgg agtccaccct tgagccacct ttttttgaat
      301 gtattcttgt tgcccatctg gtaacacaat cggacgtctt ccgcagttac gtttgttttt
      361 cttatattgt tcatagtagc ttattgcgct tccaccagat ttaagaaaac gatagacatt
      421 ataaatggtt tgtcgagaac gttgtactag atgggctact tttgaaacaa agtgtgattg
      481 attgaaataa gcttctatca tcacgagttc agttggtgta agatgggtat aggtcatttg
      541 tactcactcc ttataattct ttcgacggaa ctattttgag tgtagcacaa atgacttttt
      601 tagttgtcta gcttaatttt agaattggcg tatgcttaaa atgcggttat aggcttttgt
      661 atgaaccatt tcagtaaatt gaaaattatt tatgattgca atctcttgtt gggaacgtgc
      721 actatctcga ataatttctc ctaattcatt ggattgatag gtagcgatat tagtcaaaag
      781 aatcaacaag cgattgagct gctcttgttc ttgtaattgt aaatttgccc attttgatcg
      841 atcattttcg atggggacac gtgtatccaa ccaaaaaagt gaggttgctc tttcccatac
      901 aacgttgtct aattcatcct caatgacatt ccagttaatt gctttatagt gtaaataatt
      961 atttatcata gtacctccta aaatattgtt tgaaagggga tgatatggtg tcatttttta
     1021 ttgcattgtt tctgcaattc tctctatatc ttctgtcgta ccttttagtt caaattcatc
     1081 caacacggta aaaccaaaat catcggcgta ttgataggct gttaggcaaa attgtttatt
     1141 gaagtttctg ttgccactac caataattcc taaacaatag tctcggtttt tattgtaagt
     1201 aatcacttgg cgcaatggtg ttgtcaaaat ttcgacatct cctgtttcaa taccatttcc
     1261 accttcaaga taagtaggca gaaatacaaa ataggattca ttgatgtcaa aagtgagtgg
     1321 ttttcctaat ttgactaaat cttttacatt cacataatct accttctttt gatattgttt
     1381 ttgacaatac gtatctaaat ctgcgagaaa actcgtggta tttccactta aactaatata
     1441 atagacttta attatttgca tgtatcacac ctctttttaa taaataacca catatatagt
     1501 gcttgtttta attagaaacg ctatatatag tgtttattgt acatgacata tcgttataga
     1561 aaattgacga tggtcaatta ttttgatttt agaggagaga aaattacatg aaaaattcag
     1621 gttgtttatg caccgttcca ctcttcgcct ctctttcatt agatgaaaaa aatgagataa
     1681 agtgtttgct tcaccatcag acgtttaaaa aaggagaaat tattttttca ccaagtagtg
     1741 aagaacaact gttgattata tctttagggg ctatgaaaat atatcgaatt agtagaactg
     1801 ggaaggagca attaattcgt atcgtaaaag aaggggatta cgacggtgag aactatctct
     1861 ttggtctacc caatgactgc ctatatggtg aagctgttga agaaaccaaa acttgcactc
     1921 tctataaatc cgactttaat aaagtgatgg cacgctatcc acagctcagt tatcgattgt
     1981 tacaattaaa tgcttgcaaa acagtagctg tggaaaaaca gttgcagctt ctagcgtttg
     2041 atcgtataga ggaccggtta gccttatatc ttaaccaact atctatagca gcaaaaatgg
     2101 gggagcctat tactttacct atctctatga gagagttggc aaactatcta gctaccagtc
     2161 ctgaaactat ttctcgtaaa ttaagagatt ttcaagaaag acaattgatt actagaaaag
     2221 gaaagcagat tacactatta gaagaatttt ggtttatatt taattatata taaagaagaa
     2281 gatattaaaa ttgggattgt cattgcacga ttcaatgatt ttttaactaa aagcttacta
     2341 agtggttgtg aagatggcct tacgcgttca ggcgttttga cagaaaatat cgatgttgct
     2401 tgggtgccgg gggcctatga aatcccactt acagccgcaa cgatggctga aacaggtaaa
     2461 tatgatgcca ttattaccct aggtgcggta atcagaggag ctacaagtca ctttgatgtg
     2521 gtggttaacc aagcgtcaag tggggtatct caagtaggct taacccataa attaccggtc
     2581 atctttggta tcttaaccgt tgaaaacatc gaacaagcca ttgaacgctc tggtactaaa
     2641 gctggtaacc ttgggtttac ttatgcaacg aatactattg aaatgattaa tttattcaaa
     2701 gaaattaaac aacaataagt catcattcta tatgaaaata tctgtaacag catagggcta
     2761 gctttaattt tcaatcatgg taagatggaa gataggaaaa taaagcgcat tcaattaatt
     2821 atcaagcctt tctgatgatt tgcgcaaaaa gcttaaggag acttcatatg acgattcaat
     2881 ttaaacaaac attagcgcca accaaggata attctggaaa accaatcatt ttaagtgacg
     2941 ctaccttcgc tcaacgtaat gagaaagtcc ttcagttaat gcgtgaatat gacttctcat
     3001 cattgatgat ttatgcagat aaagaacacg gtagtaactt tgaatattta actggtttta
     3061 ttccacggtt tgaagaagct atccaagtaa tcaaccaaga cggaacgtcg acactgattt
     3121 taggtaatga gaattacaat aaaaccagta tggcacgtac acaaagtgaa ggtattcttt
     3181 gcccattctt ctctttacca aaccaaccta tggatttcac taaaactttt gctgactatc
     3241 ttaaagacgt taaagtagat gcttcaaaac gaattggttt agtagactgg aagcttttat
     3301 ctaatgatat ggacaacttc cacaaccatc attcaatacc agcatttatt gtagatggta
     3361 tacatgaagt tttcggttct gacaaggtag tgaatgcaac ccaactatac atgcatccaa
     3421 aagaaggtgc tcgtgtgaca aacaacgcag aggaaatcgc tcgttatgaa tatggtgctt
     3481 cccttgcttc tgatgctgtt cttgaagcaa tgaaccagct aaccgttggc caaagtgagt
     3541 tagcagcagg taatcactta aatcgcgatg gtcaatatcc atctgttgtg accattgcag
     3601 cctttggtga tcgctttgag ggtgctaata tttatccaac tgataatacg ctaaatgatg
     3661 gcgataaagt tgcattaact gtagcctata aaggaggctt gtctagccgc tctggctatg
     3721 cagtaaaaga ggaaagtcag ctcgctgaac atgatccagg atacttagaa gaagtggttt
     3781 acccatattt ccaagcctat aaatggtggc tagataattt aaccattggg agaaagggtg
     3841 gcgatttcta ccaagcattc agtgacttct atccgcaagc tacttatggc tggaatttgt
     3901 gcccagggca tttagtagct gacgaagaat ggttaagctc accattttat caagactctg
     3961 atgcaacaat tcaaagtggc tacatcttcc aactagactt tattccttca caagcaggcc
     4021 acaatggtgt ttcagctgaa tcaacgattg cggttgcgga cgcggcacta cgtgaacaga
     4081 ttaaggctga acaacctgaa ttatgggcac gtatctgtaa ccgaaaggat tatctaaaag
     4141 aagcattagg tttagaatta tcagaacatt tactaccctt agcgagtacc tttgcctact
     4201 atagaccttt cttattagac aaaaaaactg ctatctactt tggataaaaa aatgcccctc
     4261 aagattgttt gccaaacttg agggtttttt gtctattttt aagtgaataa tgacggacta
     4321 tccaaacgga atcgccataa agatgttaat atatgtataa tattataaag aaattggagg
     4381 acaatgccct tggacgagca gattgaatta ttatttacca tagatgaagg ctatttgaat
     4441 cccttaaaag tggctttaac atcgattcgt cagaataatc caggccaagc tttccgcatt
     4501 tggttgattc acgaatccat tgcaatgcat acgattcgcg aattgcagaa gctaacggat
     4561 tatctacagt ttggttttga agcgattaaa attgatggta gtcgatggaa tagcgctaaa
     4621 actgaagata gatacccaaa agagatgtat ttccgcctgc ttgcgggtga aatattacct
     4681 aaagaaatga aacgcgtgat ctatttagac ccagatattt tagtggttaa ccctttatta
     4741 gatttgtggc aaacagattt agaaggttat atgctagcag ctgctacaca tgttggattg
     4801 acggacgttt ctacacgagt aaaccaaatg cgacttgacg ttgaccatgc ttattacaat
     4861 tctggtgtga tggtgattga tttagataaa gctagagaaa ttattaaatg gtcagatatt
     4921 gcacaaatga ttgaaaaata taacttgcta ttaatcctac ccgaccagga tatcctcaac
     4981 cgattatatg gtaaatacac taaggaaatt ccagaagaaa tttggaatta tgatacgcgt
     5041 aaatatatgc ggtatttcac caaaagtctc gctcaacatg atattcactg ggtgatggcg
     5101 catactgcta ttctccattt ctgtggtggc ccaaaaccat gggacgataa acatgataat
     5161 cgttttacca gtctttatct tacctatcaa aatcaattaa agcgtattga agatttgatg
     5221 aagtaaagtg aaacaagtgt ttttcattac ctacggtggt ttaatgctta tttattatcg
     5281 ttaaatcatg cttatacact ttacaaaatt tgattttcga aatggccgtc caacgaagtc
     5341 tgcgatctta accacgagtt ccattgacat ggccaaaaag tattatcgag cgatcaaaga
     5401 aatgacgaaa gaacctgact ggttaacaaa agagtttcct aatcagccca ttcgtgaagg
     5461 aagaacgatg gaggatcctg attttccacg aatcgctatt acctattcat tagaagaaaa
     5521 taaccaagat tcaagtgccc aacaagaaga aatgaaaaaa attattgagg aatataatca
     5581 gtattttgat actgcctggt cattagcaga cattgagcgt tataacggag acatcaataa
     5641 ttgtttggca cgaaaaagag cggaattcaa acaatttggg aaacaaatcg atttagtaat
     5701 cgtagtggat cgtctattga caggttttga cgcacctacg attcaaaccc tatttgtgga
     5761 tcgtaatcta gagtatgcag gattgattca agctttttca cgtacgaacc gtacgtatcc
     5821 tgaaaagaca aaaggattga ttgtgacgtt tcgtaagcca gcgacaatgg agaagaatgt
     5881 cgaggatgcg acaaaactgt attcagaagc aaaagaagaa tcgggtttga tttatccaag
     5941 ttatcaggaa tccaaaaaaa gattcaagca agcgtatggt aagttaaagg aggtttcatc
     6001 catgaaatcc attgatgaac atacgccact tgaaatccgt atcgagtacg ttaaagcatt
     6061 tcaagaattg aatcgtgcgt atgaatcgtt agtgacttat gacgattaca atgatgacat
     6121 ggtgcagtct tcaactttga acaatcaaat tcaacttttg gaagaacaaa ttggcgtgta
     6181 cgagaccgtg aaggggtcat taatagagga agaaccagaa aaagagggag aagatttttc
     6241 aacgatcgaa ttttatagtg aaaataccac taagttgtat gatattgatt ctacttatat
     6301 tgatcaatta ctaggaactt atgctgcgaa tagttcagat gcacgagatg aaattgaaaa
     6361 agctttggct aaattgaata aaacagaagg agtcaaagaa gtttaccgac aaattttaaa
     6421 tgcatttgat gtggggactt tagatagcaa tgaagatatt tttgtcatta aacgtcgcta
     6481 ttttacacaa gcaagtgatg aattgattca tgccttttcc aatgagtggt ttgtttctga
     6541 gaaagaatta catgcttcta atctgcaata tatgccaggt gaagatccaa ttcctaatat
     6601 gaaagcaatt atcaacagta aggattacga aggatataaa gccaaacatc caaaagcgaa
     6661 gccatttaaa tacccacaag aaatgaagcg agcctggcgt aaaatgttag atgatgagtt
     6721 gattccactt gagaatgaat tgaggtaaac gagaaaattg aagaaattgt atgataaaca
     6781 tgttaataaa tgatgctcga aataaaattt ggttttattt ttacattgat acggtaaata
     6841 aaatttgaaa gtagaagaaa atatgaacaa caatgaaaca aaaaaaacat atgaactaag
     6901 aataactgct gatgagatta aatcaacaga cccaacattt actatgaaga atctaagctt
     6961 aggggataat tatcaaccaa aagatgctta cttccgtacg gcagaaggcg aaataactag
     7021 aagaaactac ggtaaaaatg aacggaaaat ttcaaatcaa acattaccgc gattagctat
     7081 tcaaatgttt gaacaatcga ttgtttctct accagaaagc gaaagagcaa catttcctgt
     7141 gtgtcgatat cactcaggag atgagataat acgagggatt tttccagatg ttgacagttt
     7201 caaaaaaaaa aggaagtcca ttgaattttt gatttataaa tataatgaag acaagcagat
     7261 agtcatctat tgttggagta ttttttcaac tttattattt gtacaagaat gtttgaaacg
     7321 ttttggaaca tctggggatg aatttattct tgtttataaa gggaaagaga aaaaggaaat
     7381 acgagtagga gagactcaga ctatgaccaa tcatttgctg aaatcatatg ttttacattt
     7441 actcaaatct aaaaacctta ttctacgtgg cgctcctgga acagggaaaa cattccttgc
     7501 aaatgaaatg gcagctgatg tcgtttcaaa tggcagaact acacaaatca atgaattgag
     7561 cgaagatgaa aagagccgaa ttggatttgt ccagtttcat cctagctatg attatactga
     7621 ttttgtcgaa ggtttacgtc caactacatc cgatgatggc gttgttagtt tcaccttgaa
     7681 agatggaagc tttaagagct ttgttgaaaa agcaaagaaa acaaagaaag taaatggaca
     7741 agataatttt gaagaagctt gggctaaatt tttcgatgcg gtaacggaag ctagtataga
     7801 gggagaaggc tacaataaac ttaaaacatt gacaggtaaa ccagtacgca atcttctagc
     7861 ctatgaaaga aatgaaattc aaggtgttta tcctgctaaa accaatactt atctaaacca
     7921 tgaccaaatt tataatgtct atcgtggatt gccaggtacg ccaaaaggtg gatttgatgc
     7981 ttatcgaaaa gcgattgtaa aacatttaaa agaagaattt ggcttaaaaa actacacggc
     8041 gtcaacggta gattctacga ataaccaaaa ctatgtgttt atcattgatg aaattaatcg
     8101 gggcgaaatt tccaaaattt ttggcgaatt atttttctca attgatccag gatatcgtgg
     8161 gcataaagaa ggtgtttaca ctcagtatgc aaacctccat gaaaatccag aagaaaaatt
     8221 ctatattcct aataatgttt atattattgg gacgatgaac gacattgacc gttctgttga
     8281 cacttttgat tttgctatgc gccggcgatt tactttctta gaaataaaag cagaagattc
     8341 agcacaaaat atgctgactc aagagaaaac aaaagaactt atgacgcgtt taaatgaggc
     8401 gatcgttgcc aaagaaaaag gtggattgtc aactgattac caaattggtg ctagttactt
     8461 tttaagtatc gataatggtg aggagacaat ggaacaatta tgggatacta agttatatcc
     8521 attattgaaa gactattttc atggtgaaca taaggcgaaa gaaaaattag aaatactaga
     8581 aaaggtatac tctaatgacg gtgatttaga tgaagctgaa acctctacac gataatcaag
     8641 aaatcaatga ctcagttgtg caacatccaa tcattacgaa cttagttaat cgtgacttaa
     8701 gtagtttaga gaaagaaaat tttattatct ttccgcaaca gttaaatcaa tccaaggatc
     8761 taagtgaaga taacttgata tttcaatcaa gaaatggaaa aatttggact tgtaatgtcg
     8821 taggtatcct tagcgattca acagatgaat taagaattca ttctagattc gctaatcaaa
     8881 tgaagcatga agatttcttt ttacgttata tgcttcagcg tgttttgaat tacaacgtga
     8941 ttaatgatca ttttagtgca tctaaaaaga tgacatacta cgatttgttg gtatttctct
     9001 ttccttatta tctgaatgaa gcaatgcgta aagggacata caaagaatat gtcaaacgag
     9061 aatacaataa tgccaatatc aagggagctg ttgatgttgc aaaacacatc cgttcgaatg
     9121 ttcctttcgt tggaaaagtt gcttatcgaa cacgagagtt tagctataat aatcacctaa
     9181 cccagcttat tcgtcatact attgaaaaaa tccaaaatga gtacgatttc ttacttagtg
     9241 gagatgagga tacgaaagaa aacgtggttc taatcaaaca aacaacacct ggttatgcta
     9301 gattaaatca atttaatatc cttcaagaga atatccttca tccagtcaaa catagttatt
     9361 acgaggagta ttcggctctt caacaaatct gtatccaaat tttatcggaa gaaaaatcag
     9421 gatttggaag tgataaaaat caaatacatg gaatcattat cgatgtgtct tggctatggg
     9481 aagaatacat tggaaaagtc actggttgga aacattatgg cagagataaa gggttagcaa
     9541 caatgcattt atttcaagag cccaaccggt ccccacgata tccagatttt acatttaata
     9601 atataccaat tgatacaaaa tacaaaaaac atcttgatac tagaaatgac tataatcagt
     9661 tagtcacata tattcatata atgaatttag accaagcaga tacaatgaaa ggcggctttc
     9721 tgcaacctac ttccgatccc cttcctgcaa atcatgggta taaaaaaata ggcatattaa
     9781 gcgggctagg aggagaaata ttcacatatc attttttcat tccgcaagct gcgtcaagtt
     9841 atttggaatt tgtagaacag atgagagagg tagaagcagc actacaaact acttttcaat
     9901 aagtaggaat tatagaaatt ttactcaaaa aatcttcatt aaaaaatctg actaaataag
     9961 ttgagaaaac agaatatact tcaatatcca gtcatttata aatatcattc actcatgata
    10021 cccagctccg tatcacaaat aatgatattt tttaagagaa aaaattatcc ctattttaaa
    10081 taatagcaat aggtagtggt agagagaaat ttataaataa ctttgttatt tgctttggta
    10141 gccaagtaag ttgcaaaaga aaaatagata ctgttaaaaa gataatgtag acatagaaac
    10201 aaatacgtaa aattttagtt agaatgatgg caaggagaaa aaacatgata gatgaaaaaa
    10261 agtgtttaaa aaatctattg gaagatatgg atgatattga agaaaaacta tctaaatgga
    10321 ctgggaaaac aaatatattt cagaatccgt ggtgctagtt aattttaata tcctaaagaa
    10381 tatctcaaag ttccttcaaa acgtcgttgt gctcgactta atagaacact gtaagccagt
    10441 aatatgcttt caaatctact ctctagccct ttaacagaac gtgagttgaa attttgacaa
    10501 cctaattttt ctaaggaaga aaaaacagtc tcaactgttt ttctttgttt tccaagtaag
    10561 ctgtggtcta gtttttctga tttatccatg tttttacgtg gcggaacgga tatagtaatg
    10621 cccttaaggg caagcttttc atggatttca tggctaatat atcctttgtc ccctaggata
    10681 ttgggaagat tcgcttcttc cgataaagtc tctaacactt tcacgtcat
//
LOCUS       DAFBQH010000089        10433 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.092, whole genome shotgun sequence.
ACCESSION   DAFBQH010000089 DAFBQH010000000
VERSION     DAFBQH010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.092"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            87..305
                     /locus_tag="LK694_002434"
     CDS             87..305
                     /locus_tag="LK694_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053264.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(594..1106)
                     /locus_tag="LK694_002435"
     CDS             complement(594..1106)
                     /locus_tag="LK694_002435"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBK5053265.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(1120..3417)
                     /gene="recJ"
                     /locus_tag="LK694_002436"
     CDS             complement(1120..3417)
                     /gene="recJ"
                     /locus_tag="LK694_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBK5053266.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3559..4002)
                     /locus_tag="LK694_002437"
     CDS             complement(3559..4002)
                     /locus_tag="LK694_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBK5053267.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(4003..4791)
                     /locus_tag="LK694_002438"
     CDS             complement(4003..4791)
                     /locus_tag="LK694_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK5053268.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4788..5729)
                     /locus_tag="LK694_002439"
     CDS             complement(4788..5729)
                     /locus_tag="LK694_002439"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBK5053269.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5906..6493)
                     /locus_tag="LK694_002440"
     CDS             complement(5906..6493)
                     /locus_tag="LK694_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBK5053270.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6714..8021)
                     /gene="obgE"
                     /locus_tag="LK694_002441"
     CDS             complement(6714..8021)
                     /gene="obgE"
                     /locus_tag="LK694_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBK5053271.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            8315..9325
                     /gene="gap"
                     /locus_tag="LK694_002442"
     CDS             8315..9325
                     /gene="gap"
                     /locus_tag="LK694_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBK5053272.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9410..10345)
                     /locus_tag="LK694_002443"
     CDS             complement(9410..10345)
                     /locus_tag="LK694_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK5053273.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2888 a         2356 c         1809 g         3380 t
ORIGIN      
        1 atcacttccc tctttacaaa ccgaacaaat attcgtatac taaatatacg aacaattgtt
       61 cgcataaatt aaagaggtga gaattcatga aatcaatgag acgtcttggc ttagtattaa
      121 ctgttttgtt tttaggtatt attattggta atttcggcat acgtccagct ttacttgtgt
      181 ttgttccgtt atttactttg tggttcatgt tgtgggatga aaagaagtac cgtcaatctg
      241 agcggcgcag aattcatgaa gatcatttct atcatgagcc ctactacaga tccaggcaat
      301 cataataagg attgactgct aattattcaa aagacaagaa gttaaacttt tacgggggtg
      361 tgacaaaaat cggtcacatc ctttttttcg aataaacggt gttctaaagc cagtttttcg
      421 gtattaagtc aaattgcgga aaaagtgatt ttttaaactt ctactggtct ttgcttgttt
      481 ccatttataa aaaaatagcg accctcaaaa gtcagataga gaatctgact tttgagggtc
      541 gctttaaaat cgcttcttta agaataacat caagaatagc ttcttttttc tgcttaatat
      601 tccattaatg taatgatgtc atatcctttg attttgtcgc gtccatggag atccatcaat
      661 tcgatcaaaa atgcgcagcc tacgactaca ccgcctaatt gttcgatcag ttcgatcgtc
      721 gctttgattg ttcctccagt agctagcaaa tcgtcacaaa tcagcacacg ttgacctggt
      781 ttaatggcat ccttatgcaa tgtcaatgta tctgttccat attctaatcc ataagtcact
      841 tcgatggttt ctcgaggtaa cttccctttt ttgcggactg gggcaaaacc gactcctaac
      901 tcataagcaa caggacatcc tacgatgaat ccacgggctt ctggcccaac taccatatca
      961 attcgttttt cttttgcata atttacgatt tgtttagtag cttcccgata agcgtcacca
     1021 tctgccatca gtggggaaat atctcgaaac acaattcctt ttgacggata gtcgggaata
     1081 ctggcaatat aatcttttaa atccactctt tgtttcctcc taggttgtta accatttctt
     1141 tatggtagga atatcgctca aaagcaaaaa ttcctcaact ttgattttat tgagccgtcg
     1201 ctggtataat ctgctctcag tcaaaggatg actttctggg ctagcgactt tttgtaaaac
     1261 accgttctct attgtaacaa atttcaattc aaaaaacacc tgtatcataa aaattaataa
     1321 tttttcaggg atacgcaaat attgagctat tgcttttatt ttatgacgta tatcaattcg
     1381 ttcttgagaa tggatcaatt taaacaattt tgcgtactgt tctcttgtgc ctaccccatt
     1441 caaataggcc tcatcaggtg aaatccccat cagataaaca cgattgaatg aataagaccg
     1501 aaaaacttct ttcagatccg ctaaatcatc tggacaatcc ataacaacta gagagtttgg
     1561 ttttcttttt tcaattattg cctttacaga atctagtcct tcgaaaacaa tcgcagcttc
     1621 ctggataatc tcaggcacga atttcaacgt cgaagaatca aatgctaaat aaaggctacc
     1681 ttccgacgtt cggctctgtt cacggaaacg ttttgcccgc caatcaaata cttgcagacc
     1741 acccacagaa aaatcagata ccatcagctg cggtttctta cgcccgttcc attcattgat
     1801 cgaaagcttt cctaccacat ctacctgatt attcagcagt tcttcttctt gagtaccaaa
     1861 accaaaagcg accgcatcaa gctgactcga tgcatcggaa aggacaaatt tcagatgctg
     1921 ttggtttgct ccgattcttt tgatattttc tgcttggact tgtcgaaata aaaactgagg
     1981 caaaggatta tccgtaccaa atggcgccaa tagtttcagc tcatcaattc gctctactgt
     2041 tacttcattt ggcagcaata cttcatcaat tctcaactca ggccctctca tcaagtcaat
     2101 ttgatgatcg actatatatt ggttcatctt ctcctgtagt atagtgacat tttcacttgg
     2161 catcgttaaa ccgactgctg catgatgtcc tccgaagtaa gtaaataaat ctcgcatctg
     2221 gtcaagcatt tcaaaaagat tcaaggcttc aatactacgt ccagatcctt tagctgatcc
     2281 atcttctttt aaagtaagga caagcgttgg tttacccgtt tcattcatta ttttcccagc
     2341 aacaatgccc aaaacgcctt catgccatcc tcggtttacc aatagatgga tatgattctc
     2401 cttattgacc atcgctaatg cttcttgtgt tatctgctct acgattgctt ttctttcttc
     2461 attgatctca ttcaattttt ttgcttgaac ggatgcttcc tcttcatcaa acgtcgcaag
     2521 taacgagacg gcaggacgtg gatcgcccat ccgaccgatt gcatttagtc tgggagcaat
     2581 cgaaaaaccg atcgttgttt cgtctgcgtc cctcattttt acgccgcttt cttcaaatag
     2641 cgcctgaagt cctatccgtt ctgaatgatg aatggcatcg attcctaatg aaacaaggat
     2701 tcgattctca tcagtcaatg aaactaaatc ggcgatcgtt cctatcgcta ctaaatccaa
     2761 aaattctgca ggcggttctt ccaataatgc agaagctact ttgaatgcta ctccgactcc
     2821 tgccaaatct ggaaatggat agtgccccgc tggatgacga gggtgaacaa tcgcataagc
     2881 gtctggtaaa ttctcaggta gttcgtgatg atctgtaatg atcacgtcta caccagcttg
     2941 ctgagcgtat gctaccgcat catttcctgc taccccgtta tcaacagtga tgatcaactg
     3001 gattccttct tcgatctttt cttggtatac cgattggttt ggtccatacc catgctcaaa
     3061 tcgatttggc aggaatactt ctacttctgc accaagtaat tccagtgttt ctttcattac
     3121 cgttgcactg gtgatcccat ctgcgtcata atcaccgtaa acgaggattt tttctccact
     3181 gattacagct tcttggattc ggctgattgc tttatccata tcgtgtatca gatacggatc
     3241 atgtaattct tgttcctttg gatgtaaaaa tcggttgata tccttttcct catggtaacc
     3301 tctttgccac agtaacttac cgacaaatgg agataatttc agttcacgga tcatttggag
     3361 aaaggattca gaaggctctg tctcgcttaa cttccaatca aaatttgctt gtctcacttt
     3421 ctcactccta acttagttat tatagcaaag gaaatcagca aaaaaaagca gaaagaaaga
     3481 ataaaaacag atcagatccg ctagacagat gttcagcagg acctgatctt ttgtacgacc
     3541 atttatgaaa tatccctatt agtcaaaata attgaagctt tgtttgggcg attgagtttt
     3601 ttgttcttcc ggcgctttca attgactgat ttgtgcggtt ttcgcttgta actccgcctg
     3661 ataagcagct tccaactccg cacgttcgtt tctaaactcg cgttgctgtt cttcttttgc
     3721 ttcttcaatt ttcttttgaa gttctgtttg atattctgac atttcctgag tcagctgttt
     3781 gatttgcttt ttttgctgcc atagtgttgc cgaagcagtc aaaaatacaa tcagtgcacc
     3841 aatcagcgca gaaccgatga tcactaagat caatggcgca gaaaaggaag tgaagccaaa
     3901 acttacagga acatttttat tgttcaatac agcaaaaaga acaataatca aaactaaaac
     3961 aaatccaaga atcacattgc tttgcttttt caattccttc acctactttt tgttaaaaat
     4021 tccgctggct aaataatcac caatcgttgg aaacagcgta taaaattttg atgcaacttc
     4081 taaaataaac ggttgattga tttcccgttt tggatgtctc atattgcgga cgatcatttt
     4141 tgccaacttg gcaggttcta aaacgatttg tccgactttt tccaaatagc ttccgcttgg
     4201 atcagcctta tcgaagaaat ttgtctcgat tggacctgga ttcactgtcg taacagctac
     4261 gccaagtggt ttcaattcta atcgcaaagc gttggagaag ccaaggactg caaatttcgt
     4321 ggcagaataa accgttgatt ttgcagttgc catttttcca gccatagagg cgatattaat
     4381 gatatgacct tgtcttcttt ctgccatatc gatcgcaaat ttttgtgtca aaaccatcat
     4441 tcctaagatg ttgacttcaa acatatcgta tgcttttcca aggtcgaatg taaggaagtc
     4501 ttcgaaaatc ccaaaacccg cattgtttac cagaatatcg acgcgcccga cttcttccat
     4561 caccttttca tataattcgt ccacgctttc aggattgctg acgtctacct tgaaagcata
     4621 agcctctttg ccgctcaagc gtctgcactc gtctctgaca ccttctacaa atgccattct
     4681 tcgtgcgcaa cttataacga ttgctccttg ctttgctgct tcataacaga tttgtgcgcc
     4741 cagaccgcct gaactacctg ttactaaaac aactttatct tctaaattca tgcctcttcc
     4801 tcctcgcgaa atggaatctc aatgctatca aaatctttca taatcgtcgt gttttcaaac
     4861 acttcttgtg cttctttttc aagctctaaa gctgcttttc ctaaatatct cgcactgata
     4921 tgagtaagta gcagccgttt tgcctgcgct tcttttgcaa cttcagcagc ttgatgagtc
     4981 gtggaatgga aatagttgtg cgccattctt gcttcatctt tattaaatgt gctttcatgt
     5041 accagaatat cgctattttc cgcaagcaca acggaattat gtgtttttct cgtatcgcct
     5101 aaaatagtca cgattcgtcc ttttttatct ggacctacga aatcttttcc attgatcgtc
     5161 cgcccatctt ctagttggat cgtttcacct tgcttcagtt tgccgtacag tggtccagct
     5221 ggaataccta attcttttag acgatccacc tgcaattctc ctttatgatc atgttcaacc
     5281 acacgataac caaaactgtc gatcccatga tttagttttt ttgtataaac agaaaattgc
     5341 tgatcagtaa aaatcggggc tgtttcattt agctcaatga atttcagcgg ataacttaaa
     5401 cgcgtttgcg aaatacctaa agagactttt atgtattcct ctatcccttt tggcccgtaa
     5461 atctctaatg gcgtatcccc accttgaaat gaccggctgc tgatgagccc gggaagtcca
     5521 aaaatatgat cgccgtgtaa atgtgtaatg aaaattttac cgatttttct cggacgaata
     5581 gtcgttcgca aaatctgcat ttgcgtgcct tcgccacagt cgaacaacca aacttcattg
     5641 cgctcgtcta aaagtttcaa tgctatactt gtcacatttc tatgttttgc aggaacacct
     5701 gcacctgttc ctaaaaattg taattccata atctacctga ctttctttaa gttcctcttt
     5761 aattttaggg taaaaagaag ggataatcaa ccgtaagtcg agaatgggtt caaattaaaa
     5821 ataacgttct aaagcgatag cgttttcttt gaaaattact ataaaaacag cagcgaaccc
     5881 gtttgagatc ctctgctgtt ttttattaat ttgaagatcc agaactttca ttgtcgttgg
     5941 aagtttgcgg atgtttgtcc ttccaagtct tttgtgcagc ttctttcgca tgattttcag
     6001 cttctttcgc atcatgcaca tctttttcga catcttcata atccaagcga gtaatttctc
     6061 cgcctaattc catcattacc aattgattca gtgggcgatt gtcctcttca tcagcaatca
     6121 agatcagtcc tgtagctcct tcaccgattt tatttcctac aaagtcgaac acggtctggg
     6181 catcttttat ttctttggca tcttgtgtcg caccgatcat tcctccggca aaccatccta
     6241 ataaaatccc tagcggacca cctaatacac caaccaacat tccgattaat ccacccgtag
     6301 aagatttgtt attaccggta aagtcgataa aatcttctat tttgaattga tgtgtcccat
     6361 taggatcatg gctgacaact gccatctgtt ctcctttaat cgaacgaaca gcatgtaact
     6421 ttttgatttc tgaaaatgcc tgatatgcct ggctttctac ttgaaaattt aaaatgatta
     6481 ctcgtttgct cattaaaaac gcccctttca taccttcttg ttatttattt tacctttaat
     6541 tgaaaaataa gaaaagaaaa gcactctttt tttccaataa aatagggctg tgacaaaagt
     6601 cgtatcacag ctcctttttc cgaataaacg gcgttcttga agctgatcac ttctgtcaca
     6661 acctccctta aatctgaatc tatatttttt ggtatttgtt tttatttgct tgcctattcg
     6721 acaaattcaa attcaaactc accgatacgt acaaggtcgc catctttcgc accgcgtgcc
     6781 cgaagagctt catccacacc cataccacgt aattgacgag caaaacgcat cactgtttca
     6841 tcatgatcga aattcgtcat ttggaataat ttttcgatct tctcaccaga taagatccac
     6901 gttgcatctg aatcacgatc gatttggaac tcaggacctt ctggattgaa tccatagtgt
     6961 acagtttctt cttccagttc ttcttcgtac aatgggaatt ctggcgttac ttctaacaaa
     7021 tctgctgtag cattcaataa cgcgtccaat ccttgtcgag tcactccaga aattggaaat
     7081 actgggatgt catcagcaaa ttcatcttct ttttccttgt tcagctgttc tttgaatttc
     7141 actagatttt ccggtgcatc cggcatatcc attttgtttg ccacgatgat ttgcggacgc
     7201 tctagcaagc gtaagttata agtagataat tctttgttga tggcaagata gtcttcataa
     7261 ggatctcgtc cttccatccc actcatatcg atcacatgta aaattactcg tgttctttcg
     7321 atatgacgaa ggaattgtgt acccagccca acaccttgcg aagctccttc aatcaagcct
     7381 ggcaagtctg ctgcggcaaa acttctgcca tcacttgtag taaccattcc tagattaggt
     7441 acgagcgtcg tgaaatgata agcgccgatt tttggacgag cagatgaaat aacggatagt
     7501 aaagtcgatt tcccgacaga tggaaaacct acaagtccaa cgtctgccaa tactttcaat
     7561 tctagttcga tttttcgctc ttgtcctggt tcaccgtttt cagcaatttc cggtgcagga
     7621 ttccgaggag aggcaaaacg gatattccct cgtccaccgc gtccaccttt tgccacaact
     7681 agtgtttgac cattttcaat taaatctcct aaaagagcac cagtctccgc atctcggaca
     7741 gtggttcctt gtggtacttt aacataggta tcttctgaac cacgaccgtg catcccttta
     7801 ctcatcccat tttcacctgg ctgcgctttg aaatggcgat tgaaccgaaa atccatcaat
     7861 gtacgtaacc cttcatcgac gatcaagatg acatcgccgc cacgaccacc gtctccgcca
     7921 gccggtccgc cgtcaggaac gtatttttcg cgacgaaatg caaccattcc gtctccgccc
     7981 ttgccagctt tcacatcgat cgttacttga tctaaaaaca tggacataat taagtcctcc
     8041 tattattcat ttatttattt gactgcctga atttccacaa taaacaaaag tcttacttat
     8101 tttatcggtg aaatctgatt ttgtctatca tttcagcaaa aagattgttt accttcttct
     8161 ttttttatca tttggtaagg tttccaacct tttttctgct accaagtata attttttgac
     8221 aaaatattct ttgcgtagca gaataacagt gtattaattg tattaattga ggatgccgtt
     8281 tcacagcacc cctaataaag gaggaacttt tgatatgaca gtaaaagtag gtattaacgg
     8341 atttgggcgt attggccgtc tggctttccg ccgcatcaaa gaagtctcag atgatattga
     8401 agtagtagca atcaacgact taacaagtcc aactatgtta gcacaattat tgcaatttga
     8461 ctctactcat ggtacttatc ctggcacagt tactgcaaca gaagactcaa tcgtagtgga
     8521 tggcgaagca actcgtgttt atgcagaacc cgacgcaagc aaaatccctt gggcaaaaga
     8581 aaacggtgta gatatcgtac tagaatgtac tggtttttat acatctgaag aaaaagcaaa
     8641 agcgcattta gatgctggtg tcaaacgagt agttatttct gccccagctg gtgctatgaa
     8701 aaccatcgtc tataacgtca atgatgatac attagatgaa aacgacaaaa tcatctcagc
     8761 aggttcatgt acaacaaact gtttagcacc tatggcttat ttcttaaata atgaatttgg
     8821 gatcgaagtt ggtacaatga caacagttca tgcctacact tccacacaaa tgctcttaga
     8881 tggacctgta aaaggcggta atttgcgtgc tgctcgttca gctgctgaca atactatccc
     8941 acattctact ggtgctgcga aagcaatcgg cttagttatc cctgagctac aaggaaaatt
     9001 acaaggacac gcacaacgtg ttccagtcgt tgatggttca ttaactgaat tggtttcagt
     9061 attgaaaaca aaagtaactg ctgatcaagt aaacgaagca atcaaaaaac acacagttga
     9121 caatccttca ttcgggtatg atgaccgtca aatcgtttct agcgatgtca ttggaacaac
     9181 tcaaggttcg atctttgacc caacgcaaac agaagtaaca actgctggtg atttccaact
     9241 tgttaaaact gttgcatggt atgacaatga gtatggattt acttgccaaa tgattcgctt
     9301 actagaaaaa ttcgctaact tgtaatttga tagatcaatc gataaaagaa gcctttgact
     9361 ggatccagtc aaaggcttct ttcttttcat cccctacttt taaactcgtt caatgaacat
     9421 attggcttaa aaattgttgg actacttgct gatatttttc aggatctgtc ttgtatgctt
     9481 ccgcatgctc ggctcctttt acaacatact tttcttttgg tcccttcgtt gcacgataga
     9541 ccttatctaa catttcaaat ggtacaaatg tatcttcgtc tccatgtata aagagcattg
     9601 ggcgctggtt tttcttcaat tgttccactg catttgcttc accgaaaaag tagccagcac
     9661 ggacttttgt cactagactc gttacttgca taagcggaaa tgctggaaga ctaaacatat
     9721 cgtctagctg ataagcaagc tcatcatgaa cggaagaata accgcaatct tcaatgattg
     9781 ctttcacatt atctggtagt ttttctccgc tagtcatcat aactgttgct gctcccatgc
     9841 tgacaccgta tagaacgatt tcttgcgatt ttccattgtt ttctagcact ttgtttatcc
     9901 actgtacata gtctttacgc tctggccatc caaatccaat gtaatcccct tcgctttttc
     9961 catgtcctct tgcatcagga acaaggacat tgtaacccat atcatgaaac atctttgcgt
    10021 aattggtcat cgtttcagcg tttcccatat agccatgagc cacaagagct gttttttcac
    10081 ttttcgtttc agcaggaaga taaatcgctg ataatttcag accgtctttt gaagtgatgg
    10141 tccaatcgct tcggttttct tttgaactaa accatttatg tgcatccgtt tctttggatg
    10201 tctgtgttgt accaggtgta tcttcgtcta aaaagtcttt ctcagaaggc acaacagcat
    10261 aattataaaa ataattccct gcaaacacta acgcaacaat cagagctaaa gcgacgccta
    10321 aaaggatttt catccaaagt ttcataaatc atccatctca tttctctttt ttcatataac
    10381 cttgaattag tataatcaaa gaatctctac tatgcaatgt tcatttttat aac
//
LOCUS       DAFBQH010000090         9873 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.093, whole genome shotgun sequence.
ACCESSION   DAFBQH010000090 DAFBQH010000000
VERSION     DAFBQH010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.093"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(22..267)
                     /locus_tag="LK694_002444"
     CDS             complement(22..267)
                     /locus_tag="LK694_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBK5053274.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(381..959)
                     /locus_tag="LK694_002445"
     CDS             complement(381..959)
                     /locus_tag="LK694_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBK5053275.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1036..1566)
                     /locus_tag="LK694_002446"
     CDS             complement(1036..1566)
                     /locus_tag="LK694_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBK5053276.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="LK694_002447"
     CDS             complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="LK694_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBK5053277.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2423..2818)
                     /locus_tag="LK694_002448"
     CDS             complement(2423..2818)
                     /locus_tag="LK694_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBK5053278.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2815..4227)
                     /locus_tag="LK694_002449"
     CDS             complement(2815..4227)
                     /locus_tag="LK694_002449"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBK5053279.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4224..4760)
                     /gene="cysE"
                     /locus_tag="LK694_002450"
     CDS             complement(4224..4760)
                     /gene="cysE"
                     /locus_tag="LK694_002450"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBK5053280.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4846..5085)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5082..6542)
                     /locus_tag="LK694_002451"
     CDS             complement(5082..6542)
                     /locus_tag="LK694_002451"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBK5053281.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6712..7869)
                     /locus_tag="LK694_002452"
     CDS             complement(6712..7869)
                     /locus_tag="LK694_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBK5053282.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7903..9273)
                     /gene="radA"
                     /locus_tag="LK694_002453"
     CDS             complement(7903..9273)
                     /gene="radA"
                     /locus_tag="LK694_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBK5053283.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9336..9863)
                     /locus_tag="LK694_002454"
     CDS             complement(9336..9863)
                     /locus_tag="LK694_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBK5053284.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2508 a         2214 c         1784 g         3367 t
ORIGIN      
        1 aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa aattcgatct caactgtccg
       61 agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa gaattttctt caggatccaa
      121 atcaacaacg aatactgaag gataggtttc tgttaaaacg ccgcggcgtt ctgtttgacg
      181 ctttcttcct gtctgagcaa ccaacatgat tgggctgccg atacgacctt ctaaatcttt
      241 cttgattgaa gctaaagttg ttggcataca gttcacctct actatcaact ataccacata
      301 tttttaaaat tttcaagttt accataaaaa aaaagaaatg caataaaaat aaataagaag
      361 ctcattaaga tttttctaaa ttattcctca ttataaaatt tcaagtgttt ttttattttg
      421 agttttactc tcccgtaagc gctcattact tgcttgtgat ccattgctaa acattcagcc
      481 atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc tatttttgaa
      541 aactggattt cgcaattttc gattgcttct tttaataaaa gatgctgaat cgctaaaggt
      601 tcttgcaatg tgatctctgc taaaaagtct ggtccttctg tctctatttt ttggtcgatt
      661 gagacttcat ctttttgatc acgacgcttc agcgcgttct gcttcctcaa cagagaatag
      721 atttgattgc ttataatgct tttataataa cttccaaagc taacaccttt gcttttttcg
      781 taagttctca atgcctgata taaagcgatc cttgcttctt gttcccaatc ctcagaaaca
      841 taatttctta aataataggt gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac
      901 agggcagcaa atgcttcttc gtcaccatta cgtgcagcgc gcaataactc ctcagacata
      961 aaataattcc tccataaatt cgtttatgga ggaatcttat catcaatgat tcatcgaact
     1021 gattagaacg atatgttatt tattttttct cattaaatcg tccaattttt gggacaattt
     1081 ttttaactgc tcttcattcc atggagaatt acgtcggaaa tcaataaaat gcatatcttt
     1141 attttcttgg gctattgttt tttccgtttt ttttatcgtc ttatataatt cataagcaga
     1201 agttcgtaat gctccttggg aaaagatcat ccactgttct gctaaatcgc ttgtcgccac
     1261 tgtcacttgt gtcaaacggt cattcagctg accagcgatt ctctcgatat aactgtcagc
     1321 cgtttcgtct tctttcgtaa aaacaacatc caactggtat ttcttataat tttgttggat
     1381 gcccggaact agttgggcat cgaagacaac gatgacttcc attccttcat atttcgcata
     1441 atttgataaa cgatgtaaaa gagcttctct tgcatccgcc attttgtcct gctttttaag
     1501 ctggacaagt tccggccacg aaccaatcat gttgtagccg tctacaatca acagctgttt
     1561 tttcatggtc tactccccct gacgttggga aaaagcctga tacataagca aaccagcagc
     1621 aacactggca tttaagctct ggacgtgccc ggtcatcggg atcgtcagca attcatccat
     1681 ttcttttttc aatcctggac tcattccttt gccttcatta ccaatgacta aagcaattga
     1741 tcctttggca ttccattttt ggaagtttgt gccggtcata tctgtgccaa atacccaaaa
     1801 tcctgtttct ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg caacagggac
     1861 atgttcgact gccccagtgg aagtctttgt tacgataggt gtgattccaa ctgctcggtg
     1921 ttttggtatg atcacaccat cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa
     1981 attgtgtgga tcttcgatgc tatccaaaat caaaaagaag gggtcttctt ttttctcttt
     2041 tgttcgttca atcagttggt caagggataa atattcataa ggtgtaatcg caagaacgac
     2101 tccttgatgc acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa cccatttgac
     2161 tggcacagct tgttcacgtg cgatttcttt cagttcttct actttttctc ctcgtgaatc
     2221 ttcttgaaga aagagtttat tccctcgccc ttgttgtagc gcttctactg cggcatggtg
     2281 cccaaagacg aaattatctt ctaatgcttc ttcattttga tcagaaactg actgatttcg
     2341 tccagtttgc tttttatttg aatatctatt ttgttttttg tcttcaaatc cacgtttcga
     2401 ttgatgtttg tttgttcttt ctttacccat ttttttcacc aactttctga atacaccagc
     2461 ggatcaattc ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt
     2521 cgaaccctgt tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt
     2581 tagcatttct tcctctgcga taataaagtt cttcttcttc tgtcaaaaga tgttcttcca
     2641 gcatttgctg aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat
     2701 gcagcttatt gggttttgtt tctccttgat caatcaggtg gtcacgaaca tagatttcgt
     2761 aaatcgcatc tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc
     2821 ttctcttctc catcgggttc cttgcggtgt atcttctaat aagatccctt tttctttcaa
     2881 caaatcacga atttcgtcgc ttcggacaaa atcacgattg cttcttgctt gatttcgttc
     2941 ttcgatcaat tgttcaatct cttcatctaa taactcttct gctgtaaata aaataccgaa
     3001 gatcgaaagc cattgcgaga ataaagcttc tccttgtgcc aagacagcgg cagaaacttt
     3061 cggctggtct agataagtgt tcagccactt agcaagctca tagaccactg tgatcccatt
     3121 tgctgcatta aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat tttctaacaa
     3181 agcgatttta tccgcatcat ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa
     3241 attgtcaaac gtattcttca gtcgttgtaa attggtggct gcttctttca tcgtcgtttc
     3301 actgtagcgg atcggacgac ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg
     3361 atcaacgttt ttgatcattt catgtacagt gatgaaattc cctagagatt tactcatttt
     3421 ttcatcgtct tcgccaatcg ttacatagcc attgtgcatc cagtaattag cgaatttttt
     3481 ccctgttttc gcttcacttt gtgcgatttc attttcatga tgaggaaatt ctaaatcttg
     3541 cccgccacca tggatatcga tcgtttctcc taaatgtttt gttgccataa cggaacattc
     3601 aatatgccaa ccgggacgtc ctttgcccca tggtgaatcc caagaaatct cgccaggttt
     3661 tgcttgtttc cataaagcga aatctaatgg gtcttctttt tgctgctgtt cttcccctgt
     3721 acgctgactt gctccgactt ccagttcatc gatggattgg tcacttaatt tcccataatt
     3781 ttcaaattta cgggtacggt aatacacatc acctgaagat tcataagcat agcctttttc
     3841 aaccaacact tgaataaatg ctaaaatatc tggcatatga tccatgactc ttggatgcag
     3901 tgtagcaggc tcgacattca acgctttcgt atcttcttca aatgctcgga tgaaacgatc
     3961 agcaacttct ggtgctgtaa tacctaattc gtttgctgta cggatgattt tatcgtctac
     4021 atccgtgaaa ttcgatacga aatcgacttt atagcctcga tattcaaaat agcgtcgaat
     4081 tgtatcgaaa gcaatggtgc tacgagcatt tccgatatgg atataattat acacagtcgg
     4141 tccgcagaca tacataccga catgtccctt ttgtataggg atgaattctt ctttttttct
     4201 ggtcaatgta ttgtagattt ttatcattct gatctcctca ctttttctcc tttgattcga
     4261 acgacttttg ctggtactcc taccgctgta gcgtcgtctg gaacatcttt caacacaacg
     4321 gcacctgcac cgattttggc gttttttccg atcgttaccg gtcctaatat ctgcgcattc
     4381 gcagagagca tggctccttg tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg
     4441 ccgcctaatg ttacgccatg gaataaaatc acatcgtcct cgatttctgc ggtttcgcca
     4501 atgacgattc ccattccatg gtcgatgaac acgccagttc caatcgttgc tcctggatgg
     4561 atctcaacac cagttaggaa acgccagaat tgagcattga tcttcgccaa taaaaagagc
     4621 cggtgctgat ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa accagggtat
     4681 gtcaaaacga tctcagctgt cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc
     4741 gctcgggtaa accaactcat gactttccac tcctttcgta agaattttat gtaggaaatc
     4801 atcttcttat aaagaaaaga agcgcctttt aaataagttt ccttatcaaa agacgctttc
     4861 cgcgtggttc cacttttctt cataatcaat gattatctct gacagctaac gcctgttata
     4921 cgttcttggc tactttgttt cctataaaac agtttttcct gttttctgtg tttcgccaag
     4981 accactccca gaggcatttc ggcaaccatt cattgatacc ttgcaccacc cggtatctct
     5041 cttgtaatga tcagctgctt acttttttct gttcatcgtg tttagttcag tacttgattt
     5101 aaatgtgcta atgatttttc acgaccaagc aattcgatcg tttcaggcaa ttctggacca
     5161 tgcatttgtc ctgatacagc tacacggatt ggcatgaaaa gattttttcc ttttacgcca
     5221 gtttcttttt gaacagcttt gatggcagct ttgacagttg gagcatctac tacttccatt
     5281 tgttctagct gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc agcaagtact
     5341 tcttttgctg cttcattcaa tacaggatgt tcattgaaaa atagcgtaga cagatcaacg
     5401 atttctgcag catagctcat ttgaggttga tacaggctaa cgatcttctt cacccattcg
     5461 attgtctttt catctgggtt ttctgctaca cgtccatcag cgattagata aggcagacac
     5521 atgtcagtaa atgcatctag atccattgct ttgatgtaat ggttgtttac ccattgcagt
     5581 tttttcgcat cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa cattttgatc
     5641 aattcttggt gttcgaagat ttcgtcttcg cccacaggtg accagcccaa taatgcaata
     5701 aagttgaaca ttgcatctgg tagatagcct aattcgcgat attgttcgat aaattgcaag
     5761 attgattcgt cacgtttgct tagtttttta cctgtttcac tgttgatgat caatgtcata
     5821 tgtccgaagg ttggtggtgt ccagccaaat gcttcataga tcatcagttg ttttggtgta
     5881 ttggcaatat ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc
     5941 gcaacagcaa aattataagt tggcatacca tcgcgttttt gtatgacaaa gtcgccaccg
     6001 atattatcgg attcgaacga aatagcgcct ttcaccatat ctgtaaatgc gtattctgta
     6061 ttgcgaggca cacggaaacg tacaacagat tctagacctt gtgcttcttt ttctgcttgt
     6121 tcttcaggtg tcaagttcgc acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt
     6181 tgtgcttccc gttctgcttc taattcttct tctgtgcaat agcatttgta tgctcgattg
     6241 ctagcaagca gctgatcaat cagcggttga tagatttctt tacgttcaga ttgacggtaa
     6301 gggccgtatt ctccaggatt ttcaggagat tcatcccaat tcattcccaa ccaagcaagg
     6361 ttttccaact ggcttttttc gccatcttcg atattacgtt tttggtcagt atcctcaata
     6421 cgaataataa agtctccatc attgtggcgg gcaaacaaat aattgaataa tgcagttctt
     6481 gcatttccaa tatgcaggtg cccagttgga cttggtgcat agcgtacgcg tactttcgtc
     6541 attttcttcc tcttctttca aactagactt tgactaacag caaaaattcc gtcagttacg
     6601 gcttctcaaa tatcatttat ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa
     6661 atagtaaagc caactggttg aatttcctta ccagttggct gaatttcttt tttattttga
     6721 ttgtttagtc tttttctctg tctctttttt attgtcgatt cctcgtcctg catgtgcagg
     6781 tttagcaaag atcatgcgtc cagcagctgt ttgaagggca cttgttacaa cgacttcgat
     6841 acgttcattc atgtaatgct gtccatcttc tacgacaacc atcgtgccgt catcaaggta
     6901 ggctactcct tgttggcgtt ctgtcccggc ttttactact agaacattca tcgtttcacc
     6961 agggatgacg actggtttta cggcatttgc caacgcattg atattaagaa caggtacatt
     7021 ttggaattca gagactttat tcaagttata gtcattggtt acaatgacac catctaaaag
     7081 cttagccaat ttgatcaatt tgctgtctac ttcactgata tcttcaaagt ctccatcgta
     7141 catttcgaca gatataccct cttctttttg caaggcattt aggatatcta atccgcgacg
     7201 accacgcaca cgtttcaaac tatcccctga gtccgcgatg tattgtagtt cgtaaagaac
     7261 aaaattaggg atcatcaagg ttccttctaa gaatccagtc tttgtaatat cataaatccg
     7321 accgtcaatg atcacgcttg tatccaagat tttatattta tggaaatttt ctcctgattt
     7381 tcgttctaac agttgactct caacttgact agctgtttct tgctcgattt ttttgcttcg
     7441 tgaaccaaaa actttcttcc attcttcgat tcgtgtcgtt cccattctca atcccagata
     7501 gcctaagatg atcatcagta aaatcggcaa tacactattg acgaatggaa tttccatatt
     7561 gtaaagaggg gtcgagatca caacacctaa agtcaatccg atgatcgcac cgatcgcacc
     7621 gaagagaaga taagtcaagc tgacctcact tagtttttgt tctacttgtt tgataatagc
     7681 tgagatatgt ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct
     7741 gttggtaaat gtattattca gccatccgtt tgactgttga ccgattgcct gccaagctga
     7801 gggtaaaaat gaaataccca agcttgcgcc gacaatgatc atcagcaaag taatgacacg
     7861 tttttgcatc gtttttcctc cgtttcgaca atcatttatt agttagcgga acacacgctt
     7921 caacgtttca gccaaagttg ctactccaac tatctcgatt ccttttggcg cttcccagtt
     7981 acctaagttg tttttaggta ggtatacttt tgtgaagccg agtttttgga cttctttgac
     8041 tcgttgttca atatgactga cacgtctgat ttctccagtc agtccaattt caccgatgaa
     8101 acattccgtt ggctgtgttc ctttttcttt atagcttgaa gcaatactga cagcgatagc
     8161 taaatcaatc gctggttcat ttaattttac cccacctgca gcttttaagt aagcatcttg
     8221 attctgaaga agcaacccag ctcttttttc cagcactgcc atgatcagag atacccgatt
     8281 gaaatcaaga cctgttgttg ttcgtttggc atttccgaac atagtaggtg tgactaatgc
     8341 ttggatctct accaaaattg gacgtgtccc ttccattgcc acaacgatag cagagccagt
     8401 tgcaccgtct aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc
     8461 atgctcatgc atttcaaaaa tcccaatctc attcgttgag ccaaatcggt tttttactgc
     8521 tcgcaaaatc cgaaaactat gatgttgttc accttcaaaa tataacaccg tatccaccat
     8581 atgttctaac attcttgggc cggcgatcga gccttctttg gtaacatgac cgacgataaa
     8641 aatggctatc ccatttgttt tagcgatttt gagtagttct gcagtggttt ctcttacttg
     8701 gctgacactt cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat
     8761 gataacataa tcaggggaga tatgttcgat ggcacgactg atttcgttca tatctgtttc
     8821 agcgtataga taaaattctg tgttgatact tccgaggcgt tccgcgcgta attttatctg
     8881 ctctgcgctt tcttcgccgg atacatataa gacttttccg cctatggctg ctaattgctg
     8941 agaaacttgt aacagcaaag tggatttccc gatacctgga tcgccaccaa tcaagaccat
     9001 agaccctggc accacacctc cgcctaacac acggttcagt tcttctaatt ttgttttgat
     9061 ccgaggttct tttttaggga cgacgtctgc gatttttgtt ggttttgctt tttctcctgt
     9121 caaacttgtc cgagttctgc ggtccgttgt atcttgttcg atttcttcga ccatcgtatt
     9181 ccattttccg caatttgggc agcgtcccaa gaacttaggc gacacatacc cgcaattttg
     9241 gcaaacaaat tgtacttttg atttttttgc cattgtaccg cctcctttta tcctaatcat
     9301 gagacattaa atcaagctta agtatcttta acgttttatt tactggaaga accaaatccg
     9361 ccttgacgtt gagcagtcgt ttgatcttga tctgctttta aaaacggcaa aaagatccct
     9421 tggccgatac gctcgccttt tttgatcact acatccttga aaccaaagtt ggtgaattgg
     9481 aacatgatat ggccttcatt tgcctcatta ttgtaataat cactgtcaat cacacctact
     9541 ccgtttgtca acactaaaaa ccgcttcaac ggattgcttg aacggttagc aagctgcaaa
     9601 aattcatctt ctcccatgta agctttgatt ccagtaggca cgagggttgg tttcaattgt
     9661 ttttcatttt ctgatacatc ttttttcaat ccatctgcga ctgtttccaa cccctttttc
     9721 cagcctgctt tccagatact tggaatcacg acctcttctg ctgcttcaaa atcatagcca
     9781 gcagcatgat gtgtagcgcg ctgcggtaga ttgatatttt gatctttata tttagaaata
     9841 acttcaaatc ctctttttct caccggtttt cct
//
LOCUS       DAFBQH010000091         9701 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.094, whole genome shotgun sequence.
ACCESSION   DAFBQH010000091 DAFBQH010000000
VERSION     DAFBQH010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.094"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..432)
                     /locus_tag="LK694_002455"
     CDS             complement(<1..432)
                     /locus_tag="LK694_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBK5053285.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSILFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIA"
     gene            543..814
                     /locus_tag="LK694_002456"
                     /pseudo
     CDS             543..814
                     /locus_tag="LK694_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751573.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
     gene            1353..1553
                     /locus_tag="LK694_002457"
                     /pseudo
     CDS             1353..1553
                     /locus_tag="LK694_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2040..2219
                     /locus_tag="LK694_002458"
     CDS             2040..2219
                     /locus_tag="LK694_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053286.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(2265..2870)
                     /locus_tag="LK694_002459"
     CDS             complement(2265..2870)
                     /locus_tag="LK694_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5053287.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            3052..3387
                     /locus_tag="LK694_002460"
                     /pseudo
     CDS             3052..3387
                     /locus_tag="LK694_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3552..3779
                     /locus_tag="LK694_002461"
                     /pseudo
     CDS             3552..3779
                     /locus_tag="LK694_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3905..4876
                     /locus_tag="LK694_002462"
     CDS             3905..4876
                     /locus_tag="LK694_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5053288.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICKENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            5016..6329
                     /locus_tag="LK694_002463"
     CDS             5016..6329
                     /locus_tag="LK694_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBK5053289.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            6372..7238
                     /locus_tag="LK694_002464"
     CDS             6372..7238
                     /locus_tag="LK694_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK5053290.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLMPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            7235..8083
                     /locus_tag="LK694_002465"
     CDS             7235..8083
                     /locus_tag="LK694_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK5053291.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAAFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            8097..9569
                     /locus_tag="LK694_002466"
     CDS             8097..9569
                     /locus_tag="LK694_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HBK5053292.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            9586..>9701
                     /locus_tag="LK694_002467"
     CDS             9586..>9701
                     /locus_tag="LK694_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK5053293.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEET"
BASE COUNT         3083 a         1616 c         1967 g         3035 t
ORIGIN      
        1 ggctatttga ttcaatagtt ttgcttgtct atttctaact ggtttacata atggactagg
       61 aaaactatct ataattccga ctagttcacc tttggtgagc ttcttgatga aaaagtatcg
      121 aataatcttg atagtgtaag ctaagtttga acttaagcga gtgaatcgac tcctactagg
      181 aaagtcacca ttaggaaata agatggaaca aaccgctcta tacgtggctc tttggctagt
      241 atagccatta attatgcccc atataacaca agcaataatc actgtatcgc gttgcttcag
      301 ttgatcagta tttcttcggt ttttaatctt atctggtaca caattgtggt atatgttaga
      361 aacagttctc ataatttcct tgaatgttat aaaagtatct gtataatgtt cagtatattt
      421 tgatgaacgc atatgtagtc ctcatttctt gggagttttg agactactat atgcgttttt
      481 ttgttgattt accaacatgt ttagacattt tcctctagga aaaaattaac tagcaccacg
      541 ggttaaatct atattttgat atacaaatta cagcttcaga tactaagttg cataaacctt
      601 ttccggaccc catacaaaag gcaattgatg aattgggcat aaaaaataag aaacgattta
      661 tattggtgat tcattatatg acatgcaaag tgcgaaagct tgtgggatag catttggttt
      721 agctaaatgg ggagccaaaa atatagaaaa ttttcaaagt gtagatataa ttattgattc
      781 tcctcaagaa atacttgatt tgttagaaaa atagacaaag agcgtgagat aaggataggg
      841 tctcacgctc tttgtttttc gccatttaaa gattgaaatt tttgccattt aaaaagaaaa
      901 gtgacagata ggaataaaaa cttattaaat gtagttatga tctgttatat ttcatttcta
      961 atactaggtc ttccacaagc agtgtttcct gaaatgtttt ctggagactg gggtattgtc
     1021 agctattcta tattaacatt gattgctatg gttttaggta cttcatttta taatgctttt
     1081 gtaagtaagc atgttgagga atatgaagta ttaatgtact taaaagagaa aaaaacaagt
     1141 aattaatatg agaacaccac gcatagtttt tattttgaga tattctagaa tgaaacacat
     1201 taagaacgaa agcaaagcaa aagacgccag aacttctact accttctaag ttttttcata
     1261 tttgtttgtt atgctataca tagatctgca taattagact tgcgctgccc gttattgaag
     1321 tttcaggcag cgctcttttt gggggatcga taatggaaga agaaaaaata tttgagaaac
     1381 gctggcagct agcatcaagt gaacaaagag caagatacaa taatttgatc acctcctacc
     1441 ccactgttga ctagacatat aaggaaaaga aatacttact ttggctgtgc cagttagata
     1501 tagatacctt tgaaacattt gaagtgatcc tagataaaat taaacagaat tgatcagaaa
     1561 caagcgaagc cttctctcgg ttcgcttgtt ttgctattta ttagttggct cagggcaact
     1621 ctaagctcgt ttgaaagaaa accgatggat aatccccttg tttgtaccac tttagaatac
     1681 atggatgtac gtattgccat tgactcctat tagttactga aaaaaaattt aaacaagttg
     1741 agtattgcag ataaatagct ccagtgaggt gaataactgc tccactcaag gcaatagata
     1801 gctccatcca gggcaacaaa ctgctccact ttcatattta atagatgaat aaccttctgt
     1861 ataatagaaa ggcatacctt tcggtatgac aacttataca gaatgttatt ttattggttc
     1921 actatcgaaa aattttggag ctttattttt ccggtatttc tcaacgaacg attagtttaa
     1981 ctgtgggaag ctccagacat acgattcgag aagttacttc tcgagcagac aggaaggggg
     2041 tgactgaact aaccgctgat atgagcgata cttggctgca ggaatatctt tttccggaaa
     2101 aacaaacaac agcacgaggt taccttcctg tagactggga atatgttcat aaagaactaa
     2161 tgaaaaagaa tatgacattg aaactccttc atagagaata tggggtacat ggaacataga
     2221 aacgaaaatg cacggtagaa aagcgaatga atatagtata aattctacaa atcattcgct
     2281 ttctttattt tatatatggt tggtcgagag agaccatttt ctttggcaat ttctgcaact
     2341 ggaactcctt gatttaacat gtccaccaca gttttaaaga ctaatcgttt ttgcggatct
     2401 gttgagttct cagaatattc aattggtcgc cccttatatc gaccagcttt tttggccaat
     2461 aatactcctt gccgctgacg ttcacggatt ttctcacgtt cgttttgtgc aatataactt
     2521 agcaatgata aaaacatatc gaacatagca gtatctagga gttcatttcc agtattaaaa
     2581 tctaaaaact gtgcatctag tatttttaat gctacttttt tctgtttcat aaaggtaact
     2641 gtatttttta tgtcttcata atctctgcct aggcgatcca gtgatgcaac aatgacacaa
     2701 tcaccttctc ggatataatt tagtaatttt tgaaactcag gtcgctggat atctttacca
     2761 ctggcttttt cttggaataa tttatcaatg ttaaggtctt ccatcattgc caattgtcga
     2821 tctaaatttt gatcttgtga agacactcta atatatccaa tttttgccat aaaaactcct
     2881 tttaaaaaat gatataattt acatgtaaaa tttacttgta gttttactgt tatcatagca
     2941 tttttaaaaa taaattttaa gtctacttat aatttacttt ggaggttttg aatgaatgaa
     3001 attaagcgca ataaaaattc ttttggtgtt tatttaggag agcctagctc ggaacagtta
     3061 aataaaggtg aagctcgtca tgctctatgt cgaaatatat tttatggtag aaaaggcaaa
     3121 ctttatcaaa cttattacga cggtatggag gaacagctca acgcattagg attagtaact
     3181 aatgctatca tatattggaa ttcactatat ttagaaaaag tctttgaaca aattaaaacg
     3241 gaagggttcg actgttccga agaagtgtta cgcaagcaat ttccattaat tagtgatcac
     3301 attaattttg ttggcaaata tagtttccgc tatgaaaaag aattatcgaa tggaatacta
     3361 cgtccgttaa atatagagaa ttattgagaa tgttcgtctt ttggttccta ccgtgcattt
     3421 tcgcacggat gttccagata ctatttttga atgtataaaa ttgaaaacac aagaatacta
     3481 ataaatcaac gattcttgtg tttttaattt ttcactaccg tgcattttcg tttctatgtt
     3541 ccatgtaccc caatcttgaa aaattaatta aagacttaga taaagccatt gacgacttag
     3601 tcgtggtgct accggagtat caatgtttaa ccagtatccc aggtattggt cctgtttagg
     3661 ctgctggaat actcgctgaa attggtccaa ttgaacgttt ttctgatgaa ataaaattga
     3721 caaagtatgc cggcctatat tggcgacaga gtcaatccgg taaatctgaa tacgaaaatt
     3781 tttttacgaa aagcttcacg tctaatatct attttaaaat aaaaatattc ctgctaattt
     3841 ggaatcgttt ccttcttttg gtataataat acctgaaatt cttttgagag gagttgttcg
     3901 tgaagtgaaa actactatga aggatgttgc tgcgttagca ggagttggtg tgggtacggt
     3961 atctagagta ataaatggag taaaggtgaa agaggttacc aaagaaaaag taatggctgc
     4021 aattagtgag cttaattatg agcctgatga atatgccaga ggtatgaaaa cgaatagtag
     4081 caatacaata gctcttattt tacctacgat ttggcatccc ttcttttccg agtttgccta
     4141 ctatgtagag gaagctctca gccaaaaaaa ttataagctt tttttgtgta atgcgaacgg
     4201 cagtgcggaa aaagaaaaag aatatatcca aatggtcaag cagagtaaag tagacggaat
     4261 tataggtatt acctattcag acattgataa atatgtatca tcaaatttac catttgtcag
     4321 tatagaccgc catttttcgg aacaggtttc ttatgtaacg gcagataatt atttaggtgg
     4381 gcaaattgcc gctcaagaac tacttactcg taactgtaaa aacttgtgct atgttggggg
     4441 tgtttcacct taccctaacg aaacaaacaa tcgtaaagct ggtttttaca atatttgtaa
     4501 ggaaaataaa atagctgtga aggtattgga tatgcctgaa ccaattagca atttagagga
     4561 acaattgctc gctttttttt cggataatcc tgaaattgat agcattttta ccatcaatga
     4621 tttcatggct ttggatgtga taaaagccca aaaaaaacta gggaaggaag cacctaagga
     4681 ttttcaaatt attggttttg atggagtcaa gctatcgttg caaaaagact atttactatc
     4741 aacgattgta cagccgatag aggaaatggc caaggtttca gtagaaacac tcatggcgat
     4801 gattttaaat gaagaaccgc ctaagcgtgt ggtagtaccg gttttctttc acgctggaga
     4861 gacgactaaa gagtaaaaaa ccggtttata acgatttttt tgagataggt tgacagaaag
     4921 tgatttcaat gatatcttaa aaccaattaa ttggaaacgt taccaattaa ttgaaaaata
     4981 aaacggaaac gtttcaaaaa ataggaggaa catcgatgaa aaagagaatg ttagcaattg
     5041 cagcaatttt agcattaaca gctggaggac ttgcaggatg tggcagtaag gagaaaagtt
     5101 caggtagcag tgatccagga gaaattaaga tttgggttca gttttctgat gaaacgcctg
     5161 aaggaaaagc ttgggcagag attgtgaaca attttaatga tgaatacgag ggtgaatata
     5221 aagctgttac tgagtatatt cctcgtagcg gaagcggcgg tggatatgag gataaagtaa
     5281 atgctgcaat tacaaccaat acccttcctg atgttattac gttagatgga ccgaatactg
     5341 cagcttatgc tcattcagga gtcattgctc cattagatga ttatttgaaa gatgcggaca
     5401 tggatgatgt attagacagc attattcagc aaggaaccta cgatggcaaa atgtatgcgt
     5461 ttggtttctc agagtccagt gtgggtgtct attacaataa gtcgctgttc aaagatgctg
     5521 gaattacaga agatcaattg ccgacgttag aagatccttg gacttggacg gaatttcaag
     5581 caatttgcca aaagctgact gataaataca aaacgcctgc aattgatatg caaatggcgt
     5641 cgggagacga aatgctgacc tacgcatata caccatttat atggtcgaat ggcggcgatg
     5701 tcgttaatgc tgatggaacc aaagccgaag actattttaa cggtaaaaaa tccgttgaag
     5761 cgttggaatt tattcaagaa ttggttgaaa aaggttacac cacaatcagt cctattgaaa
     5821 aaggatttga aactggcaaa tatgccatga tgctgagcgg ttcttggaca atggcagatt
     5881 tagaggaaaa ctatcaagag attgattatg gtattttgcc ttaccccgtt tcagatgcaa
     5941 ctggggagtt agtttcacca accggaagct ggcagttagc aatgactgag aaaacagaga
     6001 aaaaggatgc ttctgcggcc tttattaagt ttgcaaccag tacagaatct agtcgtatta
     6061 tgagtttagg gaacagtgtt ctacctatca gaaattcaac agttgattta attaaagatg
     6121 aagtgtcgga aggaatgcag gtattaatta agcaaaacca aatttctgga catgcccgcc
     6181 cagtggttgt agcttatccg caagtatcta gagcattcca acaagcagtt caagatatta
     6241 gctacttcga ggaaaaccca gatgtacaaa aagtagcaga tagtcgcgct gctgaaatgc
     6301 aacaggcaat tgatacttca ctaaaataga agtttctaaa gggggccaat caagtgttaa
     6361 aaaaaccgac aatgaaagac aatttgatag gttacggctt tctagcgcca gctttgctgc
     6421 tgctgacgat ttttctgatc atacccgtaa tcatggtaat ttattatgcc tttactgatt
     6481 attatttgtt aatgccagat gctagacaat ttatcggctt agaaaatttt aagcgactgt
     6541 tcgctgatcc aatttttgta aaaagtattt ggaacacggc taaatttgtt gtttggatta
     6601 ttccggtcca aataggggca gctttaggaa tggcacttat tatcaacaaa aatcgtaaag
     6661 gaaatatctt ttttaaagta gccttttttg cgccagtcgt gatgtcctta gtcgttattt
     6721 cgattttatg gttgtacttg ttaaatccta atgaaggtct ggttaatacg ctatttgcaa
     6781 agattggaat tgcttcgcag ccttttttga ctagtcccaa acaagcaatg tatacgcttg
     6841 tgttcgtatc ggcatggcaa ggtgcaggat atcagatgct gctgttttta gggggaatgc
     6901 aaaatattcc gcaagacgtt tatgaggcag ccgaaattga tggtttttct aagtttcagc
     6961 aatttcgcta tatcaccatg ccattattaa agcccacggc gatttttatt ttacttacca
     7021 cattgatttc ggcctttaaa ctaatcattc agccaatggt aatgactcaa ggtggaccaa
     7081 tgaattctac tatgacaatg gtttattaca tttaccaaac tggatttaca gaccgtatgg
     7141 taggctactc cagctccatt gcgttaatct ttaccactat gattggttta attacaattg
     7201 ctcaaagaaa attggtaaag gaggatgatc cagcatgacc aagaaatttg gaccgctaat
     7261 gatcgtagaa tattgtttgc tgttcctttt agcggcatta ttcattttcc cgatgctgtg
     7321 gatggtagtt tcctctatga agcctgaagc agatgtctat caaaatttag cttccttcaa
     7381 agctttttta cctagtttga atccagctaa ttggtttaaa acttatcaag aggttatttc
     7441 ccggttcagt gtgggaactt atttattaaa cagtattttt tatggtttaa cctttgcctg
     7501 tgggtcaatc attgtaaact ctttggcggc ttttgccttt gcgaaggtta atttttcagg
     7561 caagaaaatt atctttggag ctttgttagc tctgctgatt gtcccgatgg agacggtttt
     7621 aattccgcaa tttaccatcg ttaattcact aggcttagtg aatactcgct tggcagtgat
     7681 tttaccagcg atggccagcg cgtttaatat ctatctgttt agaaatttct ttatcgcgat
     7741 tccagaagaa atcattgaat cagcgaaatt agatggtgca aatattgtta aaattttctt
     7801 tcacatcatg ctgccaatgt ctaaaccagc catagccaca gtaggtgtct tatcctttat
     7861 ctccagctgg aatgattata tttggccgtt aatggtctta actgacagca gcaaattttc
     7921 aatgcaagtg gcaattacta cgatcaatac tacacagcca gtttatatca atcaagtgat
     7981 ggctgtttta accattgcaa cgattccact gattattgtt tatatcgttg cacaaaaata
     8041 tattctgcag ggacttggcg gttctggaac gggtataaaa taggagtgaa tagtgaatgt
     8101 tgcaaaaact tttaaaggcc aatgagttta ttgagaaaaa tgctcaaaca gtcaatcaag
     8161 aataccggaa taaatttcat ttgatggctc cgattggctg gatgaatgat ccaaatggtt
     8221 ttgtttatta tcaagaggag tatcacttgt tttatcaata ttatccttac gacagcatct
     8281 ggggaccaat gcattggggc catgctaaaa gcaaggatct aattcactgg gagcatttgc
     8341 cagtagcact ggcaccaggt gaaagctatg acaaggaagg ctgtttttcc ggcagtgcca
     8401 tagaaaagga tggcaagctg tatttgattt atacaggaca caatgttgtg gaagggcaag
     8461 ttcgacaagt tcagtgtttg gcagtttcag aagatggcat tcactttgaa aaatatgccg
     8521 gaaatccaat catcgcagaa gatcacttag cttctgttgc aacgaccgaa gatttccgcg
     8581 atcccaaagt gtttcagcga aaagaagact attttactgt agtcgctact aaaactgcag
     8641 atgctcgtgg acaaattgtg atgttccatt ccaaagattt actaacttgg gagttttatt
     8701 ccgtattgct ggagggaact ccagaacaag gcattgtatg ggaatgccca gacttgtttg
     8761 aactagatgg aaaagaagtc ttgattcttt ctcctattca aatgactcca caaggcaatg
     8821 cttaccaaaa tacgaactca actgttgctt tcatcggtac tgtcgattgg caaacaggca
     8881 aatttctagt agagaatttt catgaaatcg atggcggttt agatttttat gcaccccaga
     8941 cctgtgaaaa cgaagcaggc caaagaataa tgattgcttg gatgcaaatg tggcagcgta
     9001 aaatacctac gaatgatctg ggccatggtt ggtcaggttc catgaccttg cctagagagc
     9061 tgagggtggc tgataatcaa ttgttgcaac ggccagtttc atcaatttat caagtaattg
     9121 atccgcagga aaggattgaa tgggcgaaga ctgaagagaa tcgcttctta ctgaaggact
     9181 tagtatatga ccagcactat ttacgcttgg aaatcaattt aaaacaggca acgaaactag
     9241 aacttcacta tgcgaaggga cccgaccata cattactgct taactatgat gttgaacggg
     9301 agtgctttac tgcttccagg gaacacatgc cgtatgattt atcaggagct gaaaaggaag
     9361 tgttgaaaaa gcgcagtgtg acgattcctt tggaaaataa ttgtctaacc atcgaaattt
     9421 tccgtgatac ttcagctatt gagctttttt taaatgatcg tctgactatg agtacaactt
     9481 tttatgagac agaaaaagga caggattttc ttttgctggc agacagtgaa gtaacctttt
     9541 cattggaaag cggcttaatt aatctataga agggagagct cagtgatgat ttatggttca
     9601 atcgaagcag gcggcacaaa atttgtgtgt gccattggcg atgaagaaat gacgatcaag
     9661 gaaagagtga gctttccgac aacgacacct gaggaaacaa t
//
LOCUS       DAFBQH010000092         9317 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.095, whole genome shotgun sequence.
ACCESSION   DAFBQH010000092 DAFBQH010000000
VERSION     DAFBQH010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.095"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..2122
                     /locus_tag="LK694_002468"
     CDS             <1..2122
                     /locus_tag="LK694_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053294.1"
                     /translation="KKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQYK
                     DDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKY
                     GTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFD
                     TIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTL
                     SIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYW
                     ERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENN
                     KEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYI
                     LVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSF
                     NTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQIN
                     PYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVS
                     FTINSKKIALKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIF
                     LINLITSIIIYKKNSFKYFKENIL"
     gene            2119..2739
                     /locus_tag="LK694_002469"
     CDS             2119..2739
                     /locus_tag="LK694_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5053295.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            2853..2990
                     /locus_tag="LK694_002470"
     CDS             2853..2990
                     /locus_tag="LK694_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053296.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESY
                     C"
     gene            3432..3812
                     /locus_tag="LK694_002471"
     CDS             3432..3812
                     /locus_tag="LK694_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBK5053297.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            3794..4708
                     /locus_tag="LK694_002472"
     CDS             3794..4708
                     /locus_tag="LK694_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBK5053298.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            4948..5637
                     /locus_tag="LK694_002473"
     CDS             4948..5637
                     /locus_tag="LK694_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053299.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(5758..5844)
                     /locus_tag="LK694_002474"
     CDS             complement(5758..5844)
                     /locus_tag="LK694_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBK5053300.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
     gene            6489..7226
                     /locus_tag="LK694_002475"
     CDS             6489..7226
                     /locus_tag="LK694_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK5053301.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            7254..7793
                     /locus_tag="LK694_002476"
     CDS             7254..7793
                     /locus_tag="LK694_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBK5053302.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            7809..7979
                     /locus_tag="LK694_002477"
     CDS             7809..7979
                     /locus_tag="LK694_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053303.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            complement(8291..8752)
                     /locus_tag="LK694_002478"
     CDS             complement(8291..8752)
                     /locus_tag="LK694_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5053304.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
     gene            8885..9259
                     /locus_tag="LK694_002479"
     CDS             8885..9259
                     /locus_tag="LK694_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HBK5053305.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
BASE COUNT         3600 a         1415 c         1455 g         2847 t
ORIGIN      
        1 aaaaaaaata aacgctgttt tatcactttt tgtctgcatt acaatactat ttttttctac
       61 taatgttata aatggtataa agacttctga agcgctatat aaaaataccg ctgaaattca
      121 tatgcaatat aaagatgata tggactttaa cagttatata aacgctttgt atgaaatatc
      181 tacacaagaa aatattaata tttctcaata tagttttact gctgttaacc aactaactat
      241 tcttgctaca aaccctaacg ctaacaaaca ctggaaattt tctaaaaaaa atatattagc
      301 aagaaacgat tctaaagtcc attatactaa taacaaaaat tctaagtacc aattaaaact
      361 tccaaataat tttttaaata taaatattta tcctttttct catgtggaaa atattggatt
      421 aagtgaaata ttatatgtac aaggaaactc aaacaaatta attcctgtac tgaaaaaata
      481 tggaacacct actatttcca atatcaatca atcagatact tttcaaatta atactaatat
      541 tgttcttgtt atctgctatc ttagtatatt tattgtaatt actacaatta tatttgcttt
      601 ttcaaaaatg aaagaaatca ctctaaaaaa aatgttaggc tataactctt ttgatactat
      661 aaaagattct attaaagagt taaacctctt attttgttct ttgattataa ctttcatttt
      721 atctattttt ttgagcatat attacagtaa cgaaaatttt ttcctatcat ttcttctatg
      781 tagtattttt cattttataa ttagtttttt atttatcata gttactctaa gtatctatca
      841 tttatctagt ataaaaaatt atctaaaaaa ttcacgacca cttaagttaa atctttgtat
      901 attaaatatc tgtctattct taagcatgat ccttctgtta atagcttcta caaaagtaat
      961 aaatacccat aatgaagccg aaaataattc tcttaaatat tgggaaagaa ctactaattt
     1021 atacaaaaca aatattacta atcaattaaa tagaaacaac accgtagaag aaaataacta
     1081 cttgaaaaaa gctagtaaat ttgtttataa aatacagaag aattataaaa cttttattat
     1141 cgcgccatac aattacgcaa ctatccaaga gaataataaa gaatttttta ttggacaaaa
     1201 agtttatcca aattttgaag actatgttag tcaacctgct ggtgcaggaa tatgtgttga
     1261 tttaaactat ctaaaatatt acaatcctat ttcttttgaa gaatctactg acttaaattt
     1321 aataaactct gatccactaa ctacttacat tctagtacct aaaaaatata aagaatatgc
     1381 aaaaatgatt gaaaaaaatt atttagaaga catacagttt agacttgagg actctcctcc
     1441 tcaagctcct tataaaaatc ctaatttgaa aaatttaaaa ataaaactaa tatttgttaa
     1501 taataaccaa aagtatttta gttttaatac cttatatgga aaggcaaaag ataactatac
     1561 aataactgat cccatagtta gagttattaa tccagaaata acagaatcat tattttgggg
     1621 aaatatattg actagtgatg gtggattatt ttttgatcag aaacatactt caaaccaaaa
     1681 cttttttaat caaattaatc cgtatataaa agaatttaac ctagaaaata tcataaattt
     1741 ttcttattct gtttttcaag aaactggtga attattatct caaaaaaaag taaataaatt
     1801 aaatgcatat ctacaatatc ttttaatatt aggattattt ataataatta attttcaaat
     1861 gatttttgtt agttttacta taaattcaaa aaaaatagct ttaaaaaaaa tatttggata
     1921 ttcaccaata agtcaagttt tagatttatg tttactccct tttacaatag agttatttgc
     1981 tactatgtgt tttcaattga tattttcaaa aaacacttac ttgtatttta taatttttat
     2041 gatcttttta ataaatctaa ttacttctat tattatttat aaaaaaaata gttttaaata
     2101 ttttaaggag aatattttat gatattatca gttgagaatt tcaccctgtc aattaataaa
     2161 aaaaagatca aacaaaattg gaacttttca atatcagaaa aagattttat tatagtaaca
     2221 ggaaaaagtg gtagtggaaa aactactctg ttaaataatt taagcctaat agataaacaa
     2281 tataccggta acttattcta ttttgaaaag aaagtttcta aacatgatat acaacgtcta
     2341 cgaagaaatc atattagcta tttatttcaa aactatgctc ttcttgaaaa tcaaacagta
     2401 caatacaatt tcgaattagc aaaaaagttt aacagtaata tttccaatga tcatatttat
     2461 aatttattaa cagatttttc tttgccaaaa gatatcttaa aagaaaaaat ctttctctta
     2521 agcggtggag aacaacaaag aatagcatta attagaaata tgttaaaacc atttgatata
     2581 ttatttgcag atgaacctac aggaaattta gataaagaaa actcaacttt tataatgaac
     2641 tttctgaaaa atttaacaga aaaagaaaaa aaagccgttg tactagtaac acatgactta
     2701 aatgttctaa actatggaac taatattata gaattataat ttttacccgt gatgctagct
     2761 gatttaaaac tagcaccacg ggtatttatt ttatattcca tctctcctat cccaaaattt
     2821 aaataatcac ttaataaaaa ggaggcaccc aagtgatttt attgaaaaaa gagtatgaaa
     2881 aattttatga tagacttatt tattctttgc aattgccaaa aaaattaaac cgtcaattac
     2941 aaaagactta tgaaactaaa tcaactgaag aatgggagag ttattgctag atttttggta
     3001 gaagtaacaa aaagtggaca tattatcata atgactcgcc gaaagtctga taaaaaattg
     3061 gctaaaaata ttgctgaatg tttaataaaa ctatcagttt tagctaaata gttagtagtt
     3121 tacacaaaaa agcatttatg ttttttgttg caaagcaaca atttcatctt cggtgaaata
     3181 agcgaaactt tacaaaaagt ggctagcatt tgctagcttt ttttgtattg tggagcgtgg
     3241 cttttgaggg ggattcaagg gggagcttgc gcccctttga caagcggtta tttgcactaa
     3301 atacgtaggt atttggctag caaatataga gcttgccaaa cccttttagt gagctttact
     3361 cattgtacct ttttaggtac aatggtattg cgaacgatac gtccgtagga cgtactgaaa
     3421 gggtgggact tatgagcgaa caaaatcaaa atttggctag caattcttct aaaaaatata
     3481 cctatcgatc cgagccaaaa caaatcagtt ttcgagtgag cgaatccgaa tttgcaaagc
     3541 tgaagcaatc ggctgaagct ttgcaaatga gtgtgccggc tttcgtgaaa gccaaggcac
     3601 aaaacgcgcg tctggtgaca ccgaaagtgg cgccagacat tgcccaggca atcgcgcgcg
     3661 atttagccaa agctggaggg aatatcaatc aaattgctaa gtggtgcaat acacaccaac
     3721 atgacgttgc ttctggtgac gctcaacgtt tatccgaaaa cctaaaaatc atgcaaaagg
     3781 agctacaaaa gatatggcaa caattaaggt aagccgaacc acttcttgta gtcgtgcaat
     3841 taattacgca gaaccacgag caaccattaa aacaggaata aactgtgacg tcgattatgc
     3901 caaaagtgaa atgaaacaaa ttcgcatgct gtatggaaaa gacgaccgtg tacaggcaca
     3961 cttattgatt caatcttttc gaccaggaga aattaccgct gaaaaagcta atcaactagg
     4021 ggcagagtat gcaaaaaaaa tagcccccga tcaccaaata gctatttaca cacatacaga
     4081 caaagaccat attcataatc acattgtcat taactccgtt aatctggaga ctggaaaaaa
     4141 atttcaagct catggtcaag catttctaaa caaatgttat gacctaaatg acgagatttg
     4201 tcttgcacat aacttaccaa tcactgagcg aggaaagaaa ccagaaaaac gaaccatgtc
     4261 tgaaataaaa ttaaaagaaa aaaatgaacc tgtttggaaa gacgaaattc ggattgcgat
     4321 cgatcaaaca atgaaaaaca aaaaaaccag aacctatgac caattttgta attccctcaa
     4381 aacattgggg attcattgct tcaatcgcgg gaaaaatttc acttacgaat tgatccacaa
     4441 aaagaaaaaa gtacgatcca acagactagg aaaggactac gaaaaggaga cgattttaca
     4501 tgagctggac agacgagaac gaacctataa ccaacagcga accaacgagc tacgaagagc
     4561 tcgagctagc attcgcgaaa ctgcagaaga agtacgaaga gaacaacgaa ctcttacgcc
     4621 tgaacaaccg gaaattagac cggctcgaag aaaccatgaa caacacgttg aaagaggaac
     4681 agaccaccaa caagaattat cgctctaaaa cgttaaaaga catagaaaac gaaataaaga
     4741 aacaacgtgc ttttctcgtt ccttatacgc aagaaaggat acaaactacg attgatttac
     4801 aagttaaaaa actccatcaa gagctattag accagcgaaa taaggaacag aaaaaggtgc
     4861 ttattcaacg atcattggat tttcttgccc ccttatgttg cttagtcctg acactgttcg
     4921 cattttggtt agtatgggga ggctaaaatg gcattttatt taccctatct actcattttt
     4981 gttagtattt caggaagtat ttggctcatc tataaaattt ttcaaacaag atattcatta
     5041 aaaggctcta aaattcgatt taagcgattt ttctttcttg gctgtatatt ttccttgata
     5101 atcgtttcaa gcggcttatt cggcgtttta gagggaaata agcgcgtttc tagaagtatt
     5161 ttattaggaa atgtcactca aaagtatgaa tcagcgcgtg ataaaaagaa aaaagagcaa
     5221 gcattagcac aaaaaatgga agagtttacc acttgttatg aggaaatgaa cgatattttt
     5281 gtgaatcaag aaaaacgttt gacggataaa aacatggaaa aactcactcg tctttatcaa
     5341 aacttgccag aaaaacagca aaaagaggtt caagacaact atgagcgaac caaaaaagac
     5401 gtgcaatatg taaaagatac aaagatagaa gaagcctgta gtgatctatt tggagacaca
     5461 aacccttggt ttgccagtga agaagaaaaa aaagaaaaac aacaatctgt cacatatgaa
     5521 cgctatgaaa tcttacttca acaagcaact aacatgcaaa atccgacaaa aaaagaaacc
     5581 gccttaaact atctaaaaag tgtcaaggaa tggttagatc agcaacaaca aaattgaatt
     5641 tgatttaaaa tcatgttaaa ctgaatgtgc aaatgatcaa acattagata cacgaaagcc
     5701 ccagagaggt cggattctct ggggcttttt ttcaggttga tgtcgccttt attgatttta
     5761 cttcttgttt cggttattca accaataact gaataaagag acacaaactc ctacaagaag
     5821 ggggcaaatg aacagttcaa acattagata cacctccttc cagtcgcaag actgtcggat
     5881 aggcgacaag ttattatatc ttaaaaaatt aaatttgtgt ggtgttttta agtatcactt
     5941 gttttagggt tgttagggct tgccctgacc gcctgtaagg cgcttctcga ctagattaag
     6001 ggaaattaat caagaattgt cttagaacgg cttagacaag ctttcagagt ctcacacctc
     6061 ctctttgaac aacggtttta gcaaaggagc cggaggcgac tgcgcagtat aatatgagag
     6121 catagcgatc acaccttttt agcgaccgta gggagcaagt tttccagttt agaaaaattc
     6181 gcgaaccttt ttggaggaaa aagtaggagt tttttaggcg ggaaattcaa tgtgctctta
     6241 agatatactg ttagatatac tgttagatat actgttaata ataactgtta gggattagca
     6301 accttatggg agagtaagta ttgacatagg tagtggggga taaattgtca cactatgggg
     6361 gataaattgt cacactatgg gggataaatt gtcacactat gggggataaa ttgtcacact
     6421 atgggggata aattgtgttt tacatcctct tatgtctatg ataacataat ttattgaggt
     6481 gatttttatt gagtaacgat atagttcgat ataataatgg attaaattct gtccctttaa
     6541 gaaaatttac ccctatagat atggatttat tttggggaat atgttctaaa atgaaaagaa
     6601 aaggtacaaa agaagaaatt tttacattta aacaaataaa agaattaatt gattacaata
     6661 taaatgcacg tggcgaccgt tttgctaatg atttaataaa aatgtcaaaa aaaatcatta
     6721 ctttgtcttt tatttatgaa gacgaaaaaa catatgaaca attaaatctg tttcaaaaat
     6781 ttaaaatcga taaagtgaat gaaacattat taataaaggt ttctgaagaa tttgaatata
     6841 tacttaattc aatcggaact aattttactc gtttcgaatt agaaaatatg acaaacttaa
     6901 agagtagcta cgttaaagag ttatatcgcc aacttatggc tcataaagac agaagatcta
     6961 aaaaaggtca ctggtttgta aaaatagaag attttagagc tctattagca ataccagaaa
     7021 gttatcgcat gagagatatt gataaacaaa tttttgccca agcgaaaaaa gaatttttaa
     7081 acgttaattc aactcaccct gctattttta aaacttttga aatcaaaaaa attaaagcct
     7141 acaaaggaaa taagatagga tctctgcaaa tttattttga agaaaatata ccttcaatta
     7201 ccttagataa ttggttagag aaataatacc taacaattta ggaggcaatt atcatgcaac
     7261 ccaaagaaac ctttacaata aaggaattat ctgaattatt tgaaataagt aggcaagcaa
     7321 tctctaaaca tgttgataaa ttagacccgt ccttattagc taaaaatgag cgtggttata
     7381 aggttgtatt acgttctgga gtattgcaac ttgctagcaa cttaggtaac caaaggttac
     7441 tatataggtt gcatgagtat gaacaagaaa acgataaaaa cgtttatata tcaagcaaaa
     7501 acgtgattgt aaacgagttg ctagataagt tgcgtgaaga aaatgaattt ttacgagaac
     7561 aactttccct caaagagcaa aataattcta atatgcaaaa attactcgac cagcaacaac
     7621 agctcaccct acaggcaaat caacagatcg aaaaactgca agaacagtta caattagctt
     7681 atacggaaga aactccagaa gatgaatcaa ctactattct ttctgaagaa acagaatcga
     7741 tagaaaaaca cacaaaaaag aagaagcaaa aatggtggca actttggaaa taactggagg
     7801 gaacattcat gacaagaaaa aatcttctat tcggttctat tttactagga attctaggct
     7861 tcagtccgat gttattgtca gtaatcaata aagggaataa acatggtgaa ttgaaattga
     7921 atccaaatta ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat
     7981 aaaaaagact gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa
     8041 gcacgcgctt atgaagcgct accaaaaatt taccatattt tacaaaaaat aacaagtctt
     8101 taaaaaatga cagataaaaa tgcacaaaga actaaaaaag agaaatcgcg taaaatcaag
     8161 cattagaagc tagtaactag ctagaccaat taaaaatcac aaagtgagcg tctctatcca
     8221 ttggtagagg ggctacttgg catagtgtac attatgtcaa ctatgcaatt aaataactaa
     8281 ctacttcata ttagcttaat aaaataatca aaataactag ggggaaaaac attgtataaa
     8341 aaaatttttc attttttaaa taagaatagt aaatataaaa ataatttaag atatctatta
     8401 ctattaatcc agatagagca aaccaagtaa atattccatg tgtaaagcta aaaaaattca
     8461 tttgaatcaa ttcaaataaa cctaatatag taacaaatat attaaaaata atagctgtca
     8521 ataccagtaa attatttttt ttattttttg aattatcttc atttaaaata gaatttatgt
     8581 tagtactata aaattcacaa atttttttta gtgtctctat atctggtgtt gttttgttat
     8641 tttcccactt tgaaatagtt tgacgtgtaa cgttaaattt tcttgccaca tattcttgag
     8701 tatacccttt tttcactctt aattctctta gcttagatcc taaatcctcc attttttctc
     8761 ctatgtaaac gattatttac ataaaatttt atcataaaaa tactaacgcg ccaaaaaagt
     8821 tctttatttt ataaaacaca ctaaaataaa ataattctag taaaaaagat aggagaaata
     8881 agttatgaaa aaatttttat gtctttctgc tttaatgggt gtcttattaa ctagtggagg
     8941 aatagctagt gccacagaag ctttaaatct agatgtaccc gaagaccata ccgccattta
     9001 cggaggagga atggaagcta cagaagaagg attttcttct agtaaattga gatatgctgc
     9061 tggcggcggg gatttcaact gtggggttaa tggatttaaa gtttatgcaa attactacca
     9121 tgctagagct aaacatagtg caacagctaa aaatggacga ggcggtcaag tacgatcagt
     9181 tcagaaagct ggagtaagag cttatgcaac atgcaatgcc acacttactg gaaatactgg
     9241 ttggtggaat gtttattaaa aaagagagtt taaactctct tttttattta catttgaaag
     9301 gaaattcaaa atatgtt
//
LOCUS       DAFBQH010000093         8400 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.096, whole genome shotgun sequence.
ACCESSION   DAFBQH010000093 DAFBQH010000000
VERSION     DAFBQH010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.096"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(400..717)
                     /locus_tag="LK694_002480"
     CDS             complement(400..717)
                     /locus_tag="LK694_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK5053306.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(718..972)
                     /locus_tag="LK694_002481"
     CDS             complement(718..972)
                     /locus_tag="LK694_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK5053307.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            1331..1735
                     /gene="tnpA"
                     /locus_tag="LK694_002482"
     CDS             1331..1735
                     /gene="tnpA"
                     /locus_tag="LK694_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HBK5053308.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            1752..2900
                     /locus_tag="LK694_002483"
     CDS             1752..2900
                     /locus_tag="LK694_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5053309.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTIALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(3123..3341)
                     /locus_tag="LK694_002484"
                     /pseudo
     CDS             complement(3123..3341)
                     /locus_tag="LK694_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3607..3801)
                     /locus_tag="LK694_002485"
     CDS             complement(3607..3801)
                     /locus_tag="LK694_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053310.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(3901..3987)
                     /locus_tag="LK694_002486"
                     /pseudo
     CDS             complement(3901..3987)
                     /locus_tag="LK694_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            5153..6193
                     /locus_tag="LK694_002487"
     CDS             5153..6193
                     /locus_tag="LK694_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBK5053311.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            7017..7349
                     /locus_tag="LK694_002488"
     CDS             7017..7349
                     /locus_tag="LK694_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBK5053312.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            7968..8309
                     /locus_tag="LK694_002489"
     CDS             7968..8309
                     /locus_tag="LK694_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBK5053313.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         2977 a         1266 c         1417 g         2740 t
ORIGIN      
        1 catagatata ttggacttcc tcttttaaaa cacaaaaata aatactacct tatttaggta
       61 gtatttatta aaagttacgt caattttgat taaaaatata ctacttgaac ctctcatgag
      121 taaagtcacg aaatttttgg gaatagagcg tacttgatat cttatttttt cactaatagc
      181 agtgtctctt attcaccaag cgatccccgt agttcctacg gttcttaatt tttctctaga
      241 ctaatgattg tatctttaga ccttcggcca taacatggag caaaataaaa aggtactaag
      301 tcttatatat caatcttcga gtaaccgacg gttaacaacc atatcgagcg gcattaatca
      361 cccacctgta gaggatgagc gactttttcc taattattgt taaaagccta gtttcatttg
      421 tttagattgg aaaatacgtc ctacaagtat ctcttttttt tcatggtcga tccgataaaa
      481 taaggcatag cttttgccga tcacgatccg atatgttggg atatccattc catagatttc
      541 ggaaacttcc tcatgcatag ttggaaaatt tttggttagt tctaaagcct tctggatcgc
      601 acctacaaat ctttttatgc tggctggaga aagagcaagc tcattttccc attcttctat
      661 tatcgtttca aggctggtct gaaaactttt agaataaact agcgtgtact ctttcatcta
      721 ttcactccag ccgtattttc cgaaagcttt taccatttca atttctgaaa ctgttaaacc
      781 tttctcaaag tcttggaaac tttcttcaat caatttgcga tcgtgttctt ttgctatttg
      841 agtagtatct aatatgaata agttgtcttc ttgtttggct tggataatgt ctccgctttt
      901 taatccaaga gagcttggaa ggatcactcc cacgctattg ccgatctttc taacctttag
      961 ttcttttccc atgttatcac actcctttta gtttttgatg aataaattat aacatgttat
     1021 aacaacaatt gccaacggtg tttcttaagg gattccactc catattatta ttggacaaaa
     1081 taagccactt agaacgctga aaacatatac aaacgatatc aaaatacgag tgaactactc
     1141 accatacact tccgttaaca tccatttaac agaattaggc agaagtcgcc catcctctat
     1201 aggtgggtga tgaatgccgt tcggtatgag ggttaccgtt ggtaactcga agaccgacaa
     1261 ctaaggcgta ttgccttttt attttgtccc atgttacaat cagtctatac aagaaatgag
     1321 ttgatataac atggaattag atacaaataa tcattcagta tttcttcttt attaccacct
     1381 tgttttagta acgaaatatc gtagacaagt gattgatgat gaaatatctg attatgccaa
     1441 aactactttt gaaagaattt cagaatcgta tcacattact ttagtggagt ggaatcatga
     1501 taaagaccac gttcatatta tgttcaaagc tcaacctaaa acagaattga caaaattcat
     1561 caatgcctat aaaagtgcca gctcaagatt gataaaacgt gactttccaa gagtcaaaca
     1621 atttctttgg aaagaaatgt tctggtctaa aagcttttgt cttctgacaa ctggtggcgc
     1681 accaattgat gtgattaaga agtatatcca aaatcaagga aacaatcata aatagaaagt
     1741 aggtgaactt tatggaacaa ctaaaagcat acaaattcag aatctatcca acagaggaac
     1801 aagaaatctt ctttgccaaa tcttttggtt gtgtccgtaa ggtctacaat ctaatgcttg
     1861 atgaccgaaa aaaggcgtat gaagaagtta aaaatgattc ttctaaaaaa atgacttttc
     1921 cgacaccagc taaatacaag aaagaatttc cgtttttgaa agaaattgat agccttgctt
     1981 tagccaatgc ccaacttaat ttagataaag catacaagaa tttttttcgg gataaatccg
     2041 ttggatttcc acgtttcaaa agtaagaaaa atcctgttca gagttataca acgaataatc
     2101 aaaatggtac aattgctttg attgatagta aattcatcaa agttcctaaa ttgaagtcat
     2161 tagtaagaat taagcttcat agacaaccaa aagggattat caaatctgct acaatatcac
     2221 gtcattctag cggtaaatac tatatttctt tgttgtgtaa agaagaaatt atcgaattac
     2281 ctaaaactaa ttccacaatt ggtattgact taggtattat ggactttgcg attctttctg
     2341 atggacaaaa aattgataat aataaattca cgtccaaaat ggaaaagaaa ctaaagcgtg
     2401 aacaacgcaa attgtctagg cgtgctttat tggctaaaaa taaaggtacc aatctatttg
     2461 aagctaaaaa ttatcaaaaa caaaaacgca aagtagctag attgcatgaa aaagtaatga
     2521 atcaacgtac tgattttctg aataagttga gtacagaaat gatcaaaaat cacgatatta
     2581 tctgtattga agacttaaac acaaaaggta tgttgcgaaa tcataaatta gctaaaagta
     2641 tttctgatgt ttcttggtca aagtttgtga ctaaattaca atacaaggct gactggtatg
     2701 gacgaaaaat catcaaagta gataaatggt ttccgtctag tcagatttgt tcagagtgcg
     2761 gacataaaga tggcaagaaa tctcttgaaa ttcgagaatg gacttgtcct gtttgtcata
     2821 ctcatcatga tcgtgatatc aatgctagta tcaatattct gaccgaaggt ctaagactac
     2881 attctatggg attggcttag atccaatcat caagaaccgt aggaactacg gggatagctt
     2941 ggtaaataag agacaccgct gttagtaaag aaatacgcta tcaagtatgc tctattccca
     3001 agaagctccc acttcaagcg ttaagaaccg tcaggtttag ctaagtggtg agtagttcac
     3061 gtaggtattt ttattatata tagatttaac atatgtcggt cggttgattg atatagctaa
     3121 agttataact caaccgaagc cgattcatct acacgaataa agttgcgagt gttctcggct
     3181 aattcataaa aattgttatg tgttccatat agcaatactg gaataacttt attttcttga
     3241 atctcataag caactctata cacggtgacc gcatacttaa atccattaga ccaattacct
     3301 gataaacctc cagttttttg ctcttactta aatggatcaa tggcaatttc atcacaaacc
     3361 tatagaggat gagtgacttc tgcctaatta cgttaaaaaa tattgaaagt aactctatgt
     3421 agcaaaaaat atctagaatt tcatttcctc aaaactgatc gatattgtat ctaaaaatat
     3481 atactaccta aaataggtag tatatattaa aatatgtgct tttgttagaa aaatcaatac
     3541 ataaattatc atatctgtat atgtcattta ggtatttcag ttatgttata atagttttgc
     3601 tgtctgctac cccggcctaa tctggcaagg ggaggaggag atataatgaa ataccttttt
     3661 gattttgttt ttgcaatcaa gccacttgca gatgtagtgt gcagttacct ctgcaaatgg
     3721 cttgattgca aaattgccaa aaaagacagt aagcctaccc gttaattcta taatatagaa
     3781 acgtaaaaaa aaccccatca gcctgcacgc tgatggggtt tggtgatata atgaaatacc
     3841 tctttgttac tcataattgt accataatta gtcacgaatg caaccaatag aaaaaactac
     3901 ttagaacggt agatcatcgc taataacaat gcattgtcca ccaaatggat tttgtgaagc
     3961 attaaataca ttgttattaa ttgtcgctgc ttttaactag gcctctcccc ttctattata
     4021 aaagaagata tatatcattt cttaattacc tactacctta aaccaaaatt taagtactac
     4081 ctaatttaag tattacttaa acaatctaaa aaatagttat cccaattgtt atgcttttat
     4141 aatacgatta tttgtaacaa aatgaatatt atataattaa acactaccat atttaggtag
     4201 tgtttaatta ttagcaaaac aaaaaatatt tattctattc tcttaacttc aactaagtta
     4261 tgctgcacac ctttatataa atcatatgca actcattagc tagacaaact acaagcatgt
     4321 agttttatct gttaattatg tactacctaa ataaagtagt ttataattag caagtatata
     4381 tatttaaaaa tttaagatta atttttttat gacttgtaca aatttatctg atattctctt
     4441 attaagaact acctaatttg ggtagttctt attttttaca ttttaagtag tatttaattg
     4501 caatttggta gtattttttt attatcttat tcttagaacg gtagtactta atataaatat
     4561 aggtagtatc taatttaagt attaatggta gtatctaaat taacaggtag tatgtaacta
     4621 ctttaaagta atttttttca gattaacaca gtagtatctt attaaataag ttaattttaa
     4681 tggtagtaca taattattat atatatctga cattggtagt acttattgta ttttttagta
     4741 gtatataatc tattgttttc tagcctaaag ccttgtctta taagaataat ataatatata
     4801 ctactgaaat aaggtagtat atattactag taatggtagt atgttttata tgcataaggt
     4861 agttcttaat ttattttttg ttttgacact ggtagtactt aattatcttt aattttaaaa
     4921 ctaaatttta ttcatacgtt tttctggtag tatttaatta taaataatat ttatttaaag
     4981 tagttcttaa tgactttaaa ggtagtacat aattcatttt atttctgatt ttaggtagta
     5041 tttaattaaa aaacagtttt aatgcgtttg cattttttat ttatctttgc tattttcgaa
     5101 aagagtaggt aattttacat aaataaaaca acgtgaaagt agaggatcta caatgactga
     5161 ttttaaattt tttaaggcgg atagagttta caacgaatta ttttatcaat ttccaaaagt
     5221 ctttattgtt tctgacgaat acaaaaaaat gaaagattca actaagattg cctatatgct
     5281 tttaaaagca agattagaga tcgcaatcag caaacggcaa atcgatgaag aaggtaatgt
     5341 ttattttact tatacgacaa atgaactatg tagagtatta aactgccaaa aacaaaaagc
     5401 gatagcaatc aaaaaagagt tggaatcctt tggtttatta ttacaaaagc agatgggatt
     5461 taacaaacag ttagggaaaa ataatcctaa tagactatat ctagcagaat taaaagtctc
     5521 agaaaatgat atctacttac tcgaaaaatt tgatagagag aatagggaaa acgttgataa
     5581 atcagagggt atgaaaatca tacccaccct cgacgaaaaa tcagacgctg aatcccttgg
     5641 ggctcaagag ggtatgaaaa tcataccgtg ccaaaacgtt gataaatcag agggtatgaa
     5701 aatcatacca gaacttaata ataatatatt agacactaat agacacaata tagacactga
     5761 aaaagaccgc ctacaagatc aattgttgtt agacaatttt gagacaatta tgacaaacga
     5821 cagcattgct acgtttgtcc ctgaacgagt attaaatttg ataaaaacat tttcttcaag
     5881 ttacagtgaa gctcaaaaaa ccgtccagac tattcataat gcaaagaaaa aagctgaaat
     5941 agaaagtggt atttcgatag tttttgaaga actcgatagt tattatgtca atgcagaaca
     6001 agaattatac acgacactgt taaaagccta tcaaaaatta aaaaccgaaa aagtcgaaaa
     6061 tatccagaac ctgatttttg tctatgtaaa aaattggttt atcgaaaaac caatagctgc
     6121 taaagtatca agtgaaaaac gtttgaatta tgaaagctcc ccaagcacta ttacgaaaga
     6181 ctggttagag tgaaacttta ctgacaaagt gattgttgct tcatggagta acattaaaat
     6241 taaaaatttt ttattattct aattatatta cgaatattac aaaataatag ctattttaat
     6301 agaatttata agttaaaata ttatgataaa aatcacaaac gtagcctttt acgtcataag
     6361 tgttaaaaga aataattaat ttcaaatata gaaaagggaa cgtatgtttg catactcctt
     6421 ttttttgagt tataataata cgcgaagaga gataatttca ttatttaaac aagctaaaaa
     6481 tcttgatatt acttcttttc aggcaatacg aatattattc gcttacattt tttgtataaa
     6541 tttaattgtc tgctatgctt gtgatgaatt gattttcgtt ctgcactttt ggatgataaa
     6601 tttggacaat tgaaaataca aaaaaaagaa aggcggtatg aactcggaag gctggtaacc
     6661 ttcgtcagcg ttcatacacg gcattaagat ttggccctta atgcgttaac ctttcagtgt
     6721 tacggtatta cccgcaatgg atttagtata acagatattc aaaagtgata ccactttttt
     6781 tggctgtgtt aaggttaaaa atacgtgata ttacgatgaa tctatgccta agcacaatgt
     6841 tataaaaatg tccgtgtggc tctttgccgt atgacgaacg ctgaaattag agggaacgca
     6901 tgtttggatg cgttccctct tttttgtttg tcatgaacga aaatcaattc tctatttttg
     6961 aaaaataaca agaaaaactt gttaaaaata tttaaagaaa ttgggtggtt tattttatgt
     7021 catatacagg aattttgagt ctcaaagata tttgtcacta cggtaaacgg tgtaccgcta
     7081 cagagaaaat cacgaagaaa ctatcaactg gccaaagtaa aaccgttgtg cagtgtaaga
     7141 aatatataat ccagaaagac aaagtctcag aggaaatgat ttattacatt gggaaacaaa
     7201 agcaaataat tttaaaagat cctatcccct taaaagagct atatccaaca atcaaacatg
     7261 tttatgacca aaacggtgta ctgattggtc gaagaaaaaa tggagtcttg cgttgtaccg
     7321 caaaagggat ggggcgtttg atcggctgaa ttgatcattc tcataacttg tattttatcg
     7381 tttttcgctt gcttgaaaat attgtcgaga cttctaaagg aacttagagt cactagaaaa
     7441 ataaggagta gacttgatct atcaacaatt cccttcgggg agtgacgggg taggttttaa
     7501 cgattggtaa cgtaaaaaat tggtcaatcg agtaaatgcg cttgtttgga tagtggtgaa
     7561 atgaatttgt gtaaataata agcgcagagt aaacaagagg gaatttagaa gttagacaag
     7621 ttagaatagc gtctagatta atcatgagca gctactaagg gatctagact ttgacgcaaa
     7681 ctcatggaat aaggagtagg tcttctcgta atggggagga cgcacaagaa attgtgtcgt
     7741 gtgctgcagt acatgtagat acttgcgagg acgttgatta ttggatcaat cgagcattta
     7801 ccgctgtcta ctagggtttc tcgtttcgga cgccataaac gagtcgaaat tttttatgta
     7861 tttttaagta aacgaaaaac gataaaatat gaacaagtcc tgaataaagt gaaaaaattg
     7921 atataaaaca tagagaaaat gaaatttacg taatggagga atgacaaatg aaaaattatg
     7981 atccgaatat acgattgggc acacatacta ttaaggtttc tttccaacgt tgggattaca
     8041 aaggatttgt gacgtttaga agaggcggaa attgtaaagg attagatgtt ttagcattag
     8101 acgaggatga tttatacgat caaacattga ctgataatcc aattggattc ggcttgctac
     8161 ctgaagatga tgaaggtaat gaatggttta aaatgacgct tatgaatgat aatggagacg
     8221 aattatctgt tgaagatact tggtcatatt tgagtgatta tattgtcagc tttgaaatca
     8281 ttgaatttgt agcggataaa gaagaataaa aactggagga ataaatttta cagataaagg
     8341 cttagaagga gtcacgatga ttaataaaaa ggtagtatta ccttaggcat tgagtaagga
//
LOCUS       DAFBQH010000094         8288 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.097, whole genome shotgun sequence.
ACCESSION   DAFBQH010000094 DAFBQH010000000
VERSION     DAFBQH010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.097"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     regulatory      140..219
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            217..1410
                     /locus_tag="LK694_002490"
     CDS             217..1410
                     /locus_tag="LK694_002490"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBK5053314.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1540..3012
                     /locus_tag="LK694_002491"
     CDS             1540..3012
                     /locus_tag="LK694_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5053315.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3095..3883
                     /locus_tag="LK694_002492"
     CDS             3095..3883
                     /locus_tag="LK694_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBK5053316.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3950..4618)
                     /locus_tag="LK694_002493"
     CDS             complement(3950..4618)
                     /locus_tag="LK694_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBK5053317.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4733..6445
                     /locus_tag="LK694_002494"
     CDS             4733..6445
                     /locus_tag="LK694_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5053318.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6448..8220
                     /locus_tag="LK694_002495"
     CDS             6448..8220
                     /locus_tag="LK694_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5053319.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2538 a         1387 c         1821 g         2542 t
ORIGIN      
        1 tttaaaaagc ttagcagagt tatctttatg gatgattgtg ctaagctttt ttggacagtt
       61 ctaatgtaaa atatttttat atttttaagc aaaaaaatga aaaaatacga gcagaaaact
      121 gttttatctg ctatacttgg ttcgttgcaa gttcccgaaa ggatccgcag gacggagatg
      181 ccttgtaacc gaatttaact agggggaatc attataatgg tagaaagaca cttatttaca
      241 tcagaatctg tttctgaagg acatccagat aaaattgctg accaaatcag tgatgcaatc
      301 ttggatgcaa ttttaaaaca agatccaaca gcacgagtag cgtgtgaaac gtctgtaaca
      361 actggtcttg ttttagtatt tggagaaata tctacgacgg catatgtaga tattcaaaaa
      421 gtcgtacggg aaacaataaa agaaattggt tatacacgtg caaaattcgg atttgatgga
      481 gatacagcag ctgtattggt tgcgattgat gaacaatctc ctgatattgc tcaaggagtt
      541 gatgaagcac ttgagatccg ggatgaagat aaaaaagacg tattagatga gattggtgct
      601 ggtgaccaag gtttaatgtt tgggtttgct gtagatgaaa caccagaatt gatgccttta
      661 ccaatcgctt tgagtcatcg tctggtacga cgcttggcag atttgcgtaa atcaaatgaa
      721 ttaacgtatt tacgtccaga tgcaaaatct caagtaacgg ttgaatatga tgatcaagga
      781 caaccggaac gcgtagatac aatcgttatt tcaacacagc atgatgatgc agtagataat
      841 gaaacaattc gtcatgatgt catcgaaaaa gtagtgaagg aagttattcc agctgaatta
      901 ttagatgatc aaacgaaata ttatatcaat ccgactggcc gatttgtcat tggtggtcct
      961 caaggggatg ccggattaac aggaagaaaa atcattgttg atacgtatgg cggttatgct
     1021 cgtcatggtg gcggtgcttt ttctggtaaa gatgcgacaa aagttgaccg ttctgccagc
     1081 tatgctgcac gttatattgc taaaaacatt gttgcagcag ggcttgctcg caaagcagaa
     1141 gtacagctag cttatgcgat tggtgttgct caacctgttt cgatctcaat caatactttc
     1201 ggtactggaa cggttccgga agaagaactg attgctgcag tgagagaaaa cttcgatctt
     1261 cggccagcag gaatcatcga gatgcttgat ttgcgtcgtc cgatttacaa acaaacagct
     1321 gcttacggtc attttggtcg tacagatgta gatttgcctt gggaacatac ggacaaagta
     1381 gatgcgttga aagcaagttt agcaaaataa gttatattca gctggaacga tcgccaagtg
     1441 gtggttgttc cagctttttt tatggcagtt aacctcttga aaaagatagt aattatttat
     1501 ctatttttag atcctgtcat aatgaaaggg aggaataaga tgaaaaaaga aacaaatgtt
     1561 actttggtca cagtgagtat tttcattgca acgttcatga cggctattga aggtacaatc
     1621 gtaacaaccg ctatgccaac gattgtcggt tcattacatg gaatggaaat catgaactgg
     1681 gtattttcga tttatttgct gacaaacgct atgcttactc cgatttacgg aaaacttgcg
     1741 gataaagtgg gacggaaacc aatctttatt tttgggacat tattgtttat tatcggttcg
     1801 ttattttgcg gtttatctca aagcatgatt gggttgattg ctgctcgtgc gattcaaggg
     1861 atgggtgctg gggcaatgat gccagtagct ttgacgatca tcggcgatct ttattctatt
     1921 gaaaagcgag caaaagtttt aggtttgaat agttcagcct gggggattgc cagtgttttt
     1981 ggaccgcttg cgggaggatt catcgttgaa acgatcagct ggcactggat cttttttatc
     2041 aatgtgccga tcggaattct attaattatt ttgatttggc tatatttaaa tgaaccaaaa
     2101 atcatacatg agtctaaacc aatggatatt tttggtagtt ttagtttgat ggccatgctt
     2161 gtctcattgc tgttagggtt ccaattatta ggagacgaag gaatgagtcc gcttgtttct
     2221 gctttgctgc taggtagtat cgtaatgtta ggtatttttg tttttgccga aaaaagagct
     2281 aaagatcctg tcatcatgat gcatttattc aaaaatccac tatttgttgt ggtcaacttg
     2341 gctgcagcat tggctagtgg ttttttgatg ggaatcgatg tttatatccc tatgtggatg
     2401 caaggtgtat tagggctgaa tgcagcaatt ggtgggctgg tattagcacc attatcacta
     2461 gtctggatgg tcggttcttt ccttgcaagc aagtggatga ctcaaatgaa aactcattgg
     2521 gtcttgagaa ttggactact aatcgcagct attgggggcg cttggttgta tcttatgcct
     2581 ttcagaagta actatttatg gttttttgct atatcaacgg tcatcggtat aggcttaggt
     2641 gtgaccatga ctacctcgac tgtcagtgca caaaatagtg tatctcgtga ccagcttggt
     2701 gtagcaactt cttttaatac gttagttcga acaattggtc aaacagtcat ggtttctatt
     2761 tttggtttat tgataaatgg ggtaactaaa cgagaacttg cagcagcaaa tttaacaaat
     2821 gatgcagata tcatgaataa actggtgaat ccacaaacgg ctaaatatat tgatgcggat
     2881 attttgttac cattaagaaa aattctttat gatgggttac attccgttta tctagttggc
     2941 ttgttactcg tgatcattgc tttggtctta acgcttttcg ttcatgaaaa aagtggtaaa
     3001 ataaaacagt agtttcggtt aagatatgta cgtagccgaa tcattcaaat ttaaagttat
     3061 tcaaaaataa cttcttacta aggaaggcga aaatatggca tttattcaag caaatattta
     3121 ttcgaatgtg ttagaaatgg aagtcaatct aaatgtgata ctaccgcaaa caacaaaaaa
     3181 ggtgattggc accagttcag agcaagagac aacggatata cctgttttat atttacttca
     3241 tggaatgggt ggcaatcata gtgtatggga aagacgtacg tcgattgaac gttatgtagc
     3301 aaactacgga ttagctgtca tcatgccatc tactgatctt ggctggtaca cagatacagt
     3361 ttatgacatg aagtattgga cattcattgc agaagagcta cctgagattt gtcatgaatt
     3421 attcccgcaa ttaacaagaa aaagagaaaa aacatatgcc gctggattat cgatgggcgg
     3481 atatggcgcg ttaaaactag gccttgcaaa atcagaaaat ttcgcagcag taatctctct
     3541 ttcaggcgct gtttctgtag gaaatcgaat ggatgatctt ttgatggttc gtaaagcatc
     3601 tttttgggaa gggatctttg gaccattgga tcaaatcgaa ggttcaaaaa atgatccgca
     3661 atacttattg aaagaactag taaaaagcgg aaaaccagtt ccaagatttt atatggcttg
     3721 tggggaatct gattttcttt atcaggcaaa tcaggaaatg gtatctgcta tgcaggaaca
     3781 aggcttggcg gtcacctttg aacaaggacc tggcgatcat aactggatct tctgggacca
     3841 atggattcaa cgtgcattgg cgtggctaat ggaagaaaaa taaaaaaaga gtcttaaaaa
     3901 gatagctcct atagagatca caaacatagt gatctctata ggagcttttt tactgctcgt
     3961 tttctcttct tttattttgt tggcataagc cttcaatata ggtttgtgca ctcgttagaa
     4021 agagtgcttt ttcattcaat tctgcatggg actgcatact ttctttaaat ccctctaatt
     4081 gattttcaac tacataatta cgcataaata gaactgtttc tttcaaaaat tcatttccgt
     4141 ccataatttt ttgtcgaagc tgctgttcat gctgctgatc cattaactgt ccaatcaata
     4201 aattgtggag agaggcaatg gcctgatgac tttgggtgac tgaaaatcct tcacgaacaa
     4261 gtaattggat catttgctcc aaatgttcta atcgactcgg aaaagcgggc acagtattga
     4321 tttctaattc tgccccacaa gggaaacgtg tataaaggtc atagtaattt tccatatact
     4381 gcagcaactg ttcattccaa ggcaaagttg gatctggaag cgctaattct tcttccattg
     4441 tttcagccat tttttgcaag agctgttgtt tgtttttgaa ataccaatat aaagcaggtg
     4501 cttgaacatg aattctgttt gcgatgtttc gcatggaaag ctgcgaaaga gcttgtgttt
     4561 cagccaggat atcaaaggcg gcctgaatga tgattgcttg agaaagcttt atttccattt
     4621 tatcaacctc gtgtattgca ttatcttttt aaacatggta aattgttagt cggtctaatt
     4681 ttacagtgtt aaattagtcc tgtcaattta aacaaagggg tgtaatcaac gaatgaaatt
     4741 aatcttacga catgcgaaaa aatataaagt agctgttttt atttccttgc tgtccgtagc
     4801 agtcatggtt gcagcagcat tatggcaacc taagttacta cagcaagtat tggaagctat
     4861 tattacagaa gataatgatg aaatgaaaac aattggtatt tacctgatca gtctggcttt
     4921 gctaggatta gccgcaggtg taacgaacac gattttctct gcaaaagtag ctcaaggcgt
     4981 aagtgcagat atccgagaag aagcatttcg aaagatccag acattttcat ttggaaatat
     5041 cgaacagttc tctgctggaa atcttgttgt tcgtctaaca aatgatatca cgcaaatcca
     5101 aaatttagtg atgatttctt tgcaatcact ttttcgaatt ccattcttat ttattggtgc
     5161 cttcatttta gctatgatca cgatgccgca attatggtgg atcatcgtag cgcttatcgt
     5221 tacggtattt ctgattacgg cactttcatt tacaaggatg ggcaagcact tcatgatcat
     5281 tcaaaaactg attgatcgtg tgaatagcat tgcaaaagaa aacttactag ggattcgtgt
     5341 agtgaaatct tttgtacaag aagaaaacca attgaagtca ttcagcaaag taagtgagga
     5401 cttaacaaaa cacaacatca tagtcggtac attattttct gtgatgatcc cgtccttcat
     5461 gctggctgcc aatttggctg tggttggggc gatctttttt gtgagcgatc ttgttaaaga
     5521 tgatcctacg ttaattggtg gaattgcttc attcatgaac tacttgatgc agattatgat
     5581 ggcaatcatt attggcggaa tgatgatgat gatgacttca agagcagccg tatcgctgaa
     5641 gcggattgcg gaaattttag atgctgaacc agatcttact tatttagacg ttccagaaca
     5701 agagttgacg ggttctgtca cttttgacca tgtttctttt cgttatcctg gtgaggatac
     5761 agatacgctt aaagatatca gcttttctat caaacctgga gaaatggttg gtatcgtcgg
     5821 ggcgactggt gccgggaagt caacactagc acaattgatc cctcgtctat ttgatccgac
     5881 agaaggcaaa gttgaagtag gtggtgtaga tcttcgtcaa gtaaatgaaa agagtttaag
     5941 acaaacggtt tcctttgttt tgcaaaaagc aattttattc tcaggaacga ttgctcaaaa
     6001 cttgcgccaa gggaaaaaga atgcaacaga aaaagagatg gaacatgctt cttctattgc
     6061 tcaagcaaaa gaattcatcg aaaagttatc tgatcgttac gaagcaccaa tagaagaacg
     6121 tagcagtaac ttttctggtg gtcaaaagca acgtttatcc attactcgtg gcgttatcgg
     6181 taatccaaag atcttgatcc tagatgacag tacgagtgca ctggatgctc gctctgaaaa
     6241 gctggtccga gaagcactcg atcgagattt gaagggaaca acgacgattg tcatcgcaca
     6301 aaaaattgct tctgttgtca aagcagaccg catcttagta ttggatgaag gcagattagt
     6361 cggcgaagga acccatgagg aattggtagc tggaaaccgt gtctatcagg aaatctttga
     6421 aactcaaaaa ggaacggagg agtaagtatg actgatctaa taaaagcaag taaatttttc
     6481 tatcactatt tgaagcggta caaactctcc tttttcttta tctttatcac agtgatcatt
     6541 gctacctatc tacaagtaaa ggcacctcaa tacatcgggg aagcttttca agaattagct
     6601 aattacgtag gaggcttgat gcaaggcgta gacgataaaa gcaaatttgt ttctgtaata
     6661 tggaaactct tgctgtttta tgtactagct agtgcagcca actttatcta tagtattttg
     6721 ttcacacaag tagtagggaa gtcaacgaac cgaatgcgta tcggtctatt caacaaattg
     6781 gaaaaattga cgatccgttt ctttgattct catcaagacg gtgaaatttt aagtcgtttt
     6841 accagtgact tagataatat tcaaaatagt ttgaaccaag ctttgcttca agtcatcacg
     6901 aatgctgttt tacttgtcgg gatcttgatc atgatgttcc gccaaaatgt agagttagct
     6961 tgggctacaa ttgcttccac tccagtagcc gttttgatag ctgtcttgat tatccgcaaa
     7021 gcacggaaat atgtggatct tcagcaagat gaagtgggga aactaaatgg ttatatggat
     7081 gaaaaaatca gcggacaacg tgtcatcatc acaaatggct tacaagaaga aacgattgaa
     7141 ggattcttag agcataatca aagtgttcgc gaagcaacct tcaaaggaca agcttattct
     7201 ggtctactat ttccgatgat gcaaggaatg tcactggtca atacagcgat agttatcttt
     7261 ttcggcggct ggttagcatt aaatggtgat ttggaacgtt cggtggcatt aggcttagtt
     7321 gtgacattcg tccagtattc acaacaatat taccaaccac tgatgcagat ttcatctgga
     7381 tacagcatga tccaattagc gattacaggg gcaagacgat tgaatgaaat atttgatgaa
     7441 aaagatgaag tcaaaccaga aaacggtaaa gcatttgatg gtgttgaaaa aggtcttgcg
     7501 ttgcagcacg tagattttgg ttattctcca gaaaaactag tcttgaaaga tgtctcgatc
     7561 aatgtggaca aaggagaaat ggttgctctt gttggtccaa caggatcggg gaaaacgacg
     7621 atcatgaatc tgctaaaccg tttttacgat gtcaatgatg gttcagtagt catcgatgga
     7681 atagatatcc gagattatga tcttgatgca ttacgtagac atgtaggaat cgttttgcaa
     7741 gaatctgtat tgttctccgg aacgatcaaa gacaatatca cattcggcaa accggacgct
     7801 tcagatgaag aagtcatcaa tgcagcaaaa caagcgaata ttcatgactt catcatgacg
     7861 ttagaaaaag gttatgatac agagatatcc gaagaaaata acttattcag tacaggtcaa
     7921 aagcagttga tcagtattgc tcgaacgatc atcacaaatc catctttatt gattttggat
     7981 gaagcaacaa gtaatgtaga cacggtaaca gaagcaagga tccaaaaagc aatggatgaa
     8041 gcaatcaaag gaagaaccag ctttgtgatt gctcaccggc tgaaaacaat cttgaatgca
     8101 gatagaatcg ttgtcttgcg tgatggggaa gtcattgaag aaggcagtca tcatgagtta
     8161 ttagcagaaa atggatttta tgcggaactt tatcataatc aatttgtatt tgaaagttaa
     8221 attaaaagaa cctcgttata acggggttct ttttatatat tttatgttgt ttgtttttat
     8281 aaggggca
//
LOCUS       DAFBQH010000095         8129 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.098, whole genome shotgun sequence.
ACCESSION   DAFBQH010000095 DAFBQH010000000
VERSION     DAFBQH010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.098"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            205..1173
                     /locus_tag="LK694_002496"
     CDS             205..1173
                     /locus_tag="LK694_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBK5053320.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1166..2197
                     /gene="vanA"
                     /locus_tag="LK694_002497"
     CDS             1166..2197
                     /gene="vanA"
                     /locus_tag="LK694_002497"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBK5053321.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2203..2811
                     /locus_tag="LK694_002498"
     CDS             2203..2811
                     /locus_tag="LK694_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBK5053322.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3217..4149
                     /gene="vanY"
                     /locus_tag="LK694_002499"
     CDS             3217..4149
                     /gene="vanY"
                     /locus_tag="LK694_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HBK5053323.1"
                     /translation="MVKEETENEEVVFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYD
                     QNPKEHLENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDEL
                     INGYGLLDSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAE
                     YALPAGYSEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQ
                     YEPWHIRYVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTT
                     IHVPTNLRYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4302..4787
                     /gene="vanZ"
                     /locus_tag="LK694_002500"
     CDS             4302..4787
                     /gene="vanZ"
                     /locus_tag="LK694_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBK5053324.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5074..5403)
                     /locus_tag="LK694_002501"
     CDS             complement(5074..5403)
                     /locus_tag="LK694_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5053325.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            5708..6037
                     /locus_tag="LK694_002502"
     CDS             5708..6037
                     /locus_tag="LK694_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK5053326.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            6027..6314
                     /locus_tag="LK694_002503"
     CDS             6027..6314
                     /locus_tag="LK694_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK5053327.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(6593..7156)
                     /locus_tag="LK694_002504"
     CDS             complement(6593..7156)
                     /locus_tag="LK694_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5053328.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            7799..>8129
                     /locus_tag="LK694_002505"
     CDS             7799..>8129
                     /locus_tag="LK694_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK5053329.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSISTLYR"
BASE COUNT         2687 a         1369 c         1611 g         2462 t
ORIGIN      
        1 taatttttta ggaaaatctc aaggttatct ttactttttc ttaggaaatt aacaatttaa
       61 tattaagaaa cggctcgttc ttacacggta gacttaatac cgtaagaacg agccgttttc
      121 gttcttcaga gaaagatttg acaagattac cattggcatc cccgttttat ttggtgcctt
      181 tcacagaaag ggttggtctt aattatgaat aacatcggca ttactgttta tggatgtgag
      241 caggatgagg cagatgcatt ccatgctctt tcgcctcgct ttggcgttat ggcaacgata
      301 attaacgcca acgtgtcgga atccaacgcc aaatccgcgc ctttcaatca atgtatcagt
      361 gtgggacata aatcagagat ttccgcctct attcttcttg cgctgaagag agccggtgtg
      421 aaatatattt ctacccgaag catcggctgc aatcatatag atacaactgc tgctaagaga
      481 atgggcatca ctgtcgacaa tgtggcgtac tcgccggata gcgttgccga ttatactatg
      541 atgctaattc ttatggcagt acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat
      601 gatttcaggt tggacagcga ccgtggcaag gtactcagcg acatgacagt tggtgtggtg
      661 ggaacgggcc agataggcaa agcggttatt gagcggctgc gaggatttgg atgtaaagtg
      721 ttggcttata gtcgcagccg aagtatagag gtaaactatg taccgtttga tgagttgctg
      781 caaaatagcg atatcgttac gcttcatgtg ccgctcaata cggatacgca ctatattatc
      841 agccacgaac aaatacagag aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt
      901 ccacttgtag atacctatga gttggttaaa gcattagaaa acgggaaact gggcggtgcc
      961 gcattggatg tattggaagg agaggaagag tttttctact ctgattgcac ccaaaaacca
     1021 attgataatc aatttttact taaacttcaa agaatgccta acgtgataat cacaccgcat
     1081 acggcctatt ataccgagca agcgttgcgt gataccgttg aaaaaaccat taaaaactgt
     1141 ttggattttg aaaggagaca ggagcatgaa tagaataaaa gttgcaatac tgtttggggg
     1201 ttgctcagag gagcatgacg tatcggtaaa atctgcaata gagatagccg ctaacattaa
     1261 taaagaaaaa tacgagccgt tatacattgg aattacgaaa tctggtgtat ggaaaatgtg
     1321 cgaaaaacct tgcgcggaat gggaaaacga caattgctat tcagctgtac tctcgccgga
     1381 taaaaaaatg cacggattac ttgttaaaaa gaaccatgaa tatgaaatca accatgttga
     1441 tgtagcattt tcagctttgc atggcaagtc aggtgaagat ggatccatac aaggtctgtt
     1501 tgaattgtcc ggtatccctt ttgtaggctg cgatattcaa agctcagcaa tttgtatgga
     1561 caaatcgttg acatacatcg ttgcgaaaaa tgctgggata gctactcccg ccttttgggt
     1621 tattaataaa gatgataggc cggtggcagc tacgtttacc tatcctgttt ttgttaagcc
     1681 ggcgcgttca ggctcatcct tcggtgtgaa aaaagtcaat agcgcggacg aattggacta
     1741 cgcaattgaa tcggcaagac aatatgacag caaaatctta attgagcagg ctgtttcggg
     1801 ctgtgaggtc ggttgtgcgg tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga
     1861 ccaaatcagg ctgcagtacg gaatctttcg tattcatcag gaagtcgagc cggaaaaagg
     1921 ctctgaaaac gcagttataa ccgttcccgc agacctttca gcagaggagc gaggacggat
     1981 acaggaaacg gcaaaaaaaa tatataaagc gctcggctgt agaggtctag cccgtgtgga
     2041 tatgttttta caagataacg gccgcattgt actgaacgaa gtcaatactc tgcccggttt
     2101 cacgtcatac agtcgttatc cccgtatgat ggccgctgca ggtattgcac ttcccgaact
     2161 gattgaccgc ttgatcgtat tagcgttaaa ggggtgataa gcatggaaat aggatttact
     2221 tttttagatg aaatagtaca cggtgttcgt tgggacgcta aatatgccac ttgggataat
     2281 ttcaccggaa aaccggttga cggttatgaa gtaaatcgca ttgtagggac atacgagttg
     2341 gctgaatcgc ttttgaaggc aaaagaactg gctgctaccc aagggtacgg attgcttcta
     2401 tgggacggtt accgtcctaa tcgtgctgta aactgtttta tgcaatgggc tgcacagccg
     2461 gaaaataacc tgacaaagga aagttattat cccaatattg accgaactga gatgatttca
     2521 aaaggatacg tggcttcaaa atcaagccat agccgcggca gtgccattga tcttacgctt
     2581 tatcgattag acacgggtga gcttgtacca atggggagcc gatttgattt tatggatgaa
     2641 cgctctcatc atgcggcaaa tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg
     2701 cgctccatca tggaaaacag tgggtttgaa gcatatagcc tcgaatggtg gcactatgta
     2761 ttaagagacg aaccataccc caatagctat tttgatttcc ccgttaaata aacttttaac
     2821 cgttgcacgg acaaactata taagctaact ctttcggcag gaaacccgac gtatgtaact
     2881 ggttcttagg gaatttatat atagtagata gtattgaaga tgtaaggcag agcgatattg
     2941 cggtcattat ctgcgtgcgc tgcggcaaga tagcctgata ataagactga tcgcatagag
     3001 gggtggtatt tcacaccgcc cattgtcaac aggcagttca gcctcgttaa attcagcatg
     3061 ggtatcactt atgaaaattc atctacattg gtgataatag taaatccagt agggcgaaat
     3121 aattgactgt aatttacggg gcaaaacggc acaatctcaa acgagattgt gccgtttaag
     3181 gggaagattc tagaaatatt tcatacttcc aactatatag ttaaggagga gactgaaaat
     3241 gaagaagttg tttttttatt gttattgtta ttcttaatat acttaggtta tgactacgtt
     3301 aatgaagcac tgttttctca ggaaaaagtc gaatttcaaa attatgatca aaatcccaaa
     3361 gaacatttag aaaatagtgg gacttctgaa aatacccaag agaaaacaat tacagaagaa
     3421 caggtttatc aaggaaatct gctattaatc aatagtaaat atcctgttcg ccaagaaagt
     3481 gtgaagtcag atatcgtgaa tttatctaaa catgacgaat taataaatgg atacgggttg
     3541 cttgatagta atatttatat gtcaaaagaa atagcacaaa aattttcaga gatggtcaat
     3601 gatgctgtaa agggtggcgt tagtcatttt attattaata gtggctatcg agactttgat
     3661 gagcaaagtg tgctttacca agaaatgggg gctgagtatg ccttaccagc aggttatagt
     3721 gagcataatt caggtttatc actagatgta ggatcaagct tgacgaaaat ggaacgagcc
     3781 cctgaaggaa agtggataga agaaaatgct tggaaatacg ggttcatttt acgttatcca
     3841 gaggacaaaa cagagttaac aggaattcaa tatgaaccat ggcatattcg ctatgttggt
     3901 ttaccacata gtgcgattat gaaagaaaag aatttcgttc tcgaggaata tatggattac
     3961 ctaaaagaag aaaaaaccat ttctgttagt gtaaatgggg aaaaatatga gatcttttat
     4021 tatcctgtta ctaaaaatac caccattcat gtgccgacta atcttcgtta tgagatatca
     4081 ggaaacaata tagacggtgt aattgtgaca gtgtttcccg gatcaacaca tactaattca
     4141 aggaggtaag gatggcggaa tgaaaccaac gaaattaatg aacagcatta ttgtactagc
     4201 acttttgggg taacgttagc tttttaattt aaaacccacg ttaactagga cattgctata
     4261 ctaatgatac aacttaaaca aaagaattag aggaaattat attgggaaaa atattatcta
     4321 gaggattgct agctttatat ttagtgacac taatctggtt agtgttattc aaattacaat
     4381 acaatatttt atcagtattt aattatcatc aaagaagtct taacttgact ccatttactg
     4441 ctactgggaa tttcagagag atgatagata atgttataat ctttattcca tttggcttgc
     4501 ttttgaatgt caattttaaa gaaatcggat ttttacctaa gtttgctttt gtactggttt
     4561 taagtcttac ttttgaaata attcaattta tcttcgctat tggagcgaca gacataacag
     4621 atgtaattac aaatactgtt ggaggctttc ttggactgaa attatatggt ttaagcaata
     4681 agcatatgaa tcaaaaaaaa ttagacagag ttattatttt tgtaggtata cttttgctcg
     4741 tattattgct cgtttaccgt acccatttaa gaataaatta cgtgtaagat gtctaaatca
     4801 agcaatctga tctttcatac acataaagat attgaatgaa ttggattaga tggaaaacgg
     4861 gatgtgggga aactcgcccg taggtgtgaa gtgaggggaa aaccggtgat aaagtaaaaa
     4921 gcttacctaa cactatagta acaaagaaag cccaattatc aattttagtg ctgaggaatt
     4981 ggtctcttta ataaatttcc ttaacgttgt aaatccgcat tttcctgacg gtacccccaa
     5041 actaagattt catagataga ttttctgata actttaaaaa acacgaatag cggaaaggat
     5101 gtcttcattc gctaaagagt aataaactac ttttccatct tgacgtgatt ttgccattcc
     5161 atgttttttt aaaaaacgta aatggtgtga cgttgtagcg acactagcag taagtatttc
     5221 tgctaaatca cagacgcaca tttccttata ggtttctaaa gcatagaaaa ttttaattct
     5281 agaaaaatcg ctgaaacatt tccctaacac aagtaagttt gaaaaatctt tttgatccaa
     5341 tttatttttt attccattta ctttttcttt atgtacgaca gtcacttggc aaactttttt
     5401 cattcatctc acctcacatt caaacgatta tttgaatgaa gtatacctat ttgatcactc
     5461 ttttgtcaat ctaccttaat tccaatacac tattataagc aatttcatgt attgaagtaa
     5521 acttacttgc aagtgcgttt ttgattccaa gttaaaaaca tattattatt ctacataatt
     5581 tcctaatcaa ataaaactcc tttgaaaatt gttctaaagg agttttatta ttttgtattt
     5641 ctactaaaaa tagcgtacaa tgtaattact aagtaattac gaaatgtaaa ataatggagg
     5701 aaataatatg attacaaaaa caagaaaaca aggaaattct attatgttaa cggtccctaa
     5761 agaattcgat gtgcccaatg gtgttgaagt agaagctaag cttgtagaga atggtatcct
     5821 ttatgagttt gtagagccta aaaaagaatt ttttgatttt agtgaagata ttttggctga
     5881 tattctttca gaaggttata gcaaacaaga gatcttaaaa gaatttagaa atagaaaaaa
     5941 tgcgttaact tctgcattta aatctattgc tgaagatact gttgcaaatt ctaaaccgat
     6001 gacaaaagag gagttggcag cagaaattgg cttatgagat tctaccttct aaacacgtaa
     6061 taaagtatct aaaaaaatta aaagagaaaa cattaaaaga acaattttta acgataattt
     6121 atgatgagat agcagttcgt cctcattcgg gagaacaaaa aacgggtgat ttatcaggaa
     6181 tttgggctat tggattcaag tatgcaggta cgacatatcg cgttgcatat gaaattaaag
     6241 ataatgcagt gatacctatc ttactatgtg gaacgcatga aaatttttat gaacaattaa
     6301 aaaagattag gtaagaaccc aatgtttcaa cttgagagga tagacgacac tttaaaagat
     6361 aatttttaac agctgagagt tgtaataccc tataatttta aaccactaac ctatatattt
     6421 atgaaaaata gaaaaaatta aaacgcattt taagcctctt tcaaattaaa agcttcatcc
     6481 ttatgcacaa agagggctta aaatgcgtta ttttatattg ttttttagta ataataaaaa
     6541 aatcactcta attctcccat aaatattata gattcctaaa gattaaaata gattattccc
     6601 tattgtcttt taagtagcgg tagagtgttg ccttgctaat accagtcatt tccgtaattt
     6661 cttttacgct ataaacctga cttttataca attttactgc tcgatcaaca tctttctggt
     6721 tcactcgtgg acggccacct ttacgacctc tcgcacgtgc acttttcaaa ccttctttgg
     6781 tacgttcaac aattaaatca cgttcgaact gactaaatgc ttggaagaca gtcatcatta
     6841 agcgtccgtg aggcgtgctg gtatcgaagt tttctttcaa gctcaccagc ttaacatggt
     6901 gttcttcaaa ataggtaaca aggtcaatca aatcctttgt actacgaccc aatcgagaaa
     6961 aactttctac aattaccgta tctcccggac gaattttatc ttttaaacga tttagttggg
     7021 gacgaaccgc tttggttccc gtcattttct cagttaggat ttcattacaa ttttgttcat
     7081 tgagtaagtc taattgacga tttagttctt gtgaacgtgt actcactcgg gcataaccat
     7141 aaatgtatcc actcatgctt ttcttccttt cgtactttaa ttctggcttg attgtatcat
     7201 aaaagggagt tatagataca tagttaatgc aacaagttat gatacaaaat atgacctctt
     7261 ttatttaata aaagaggtca tttcattttg tctcataatc gattgttttt gatacgagtg
     7321 tgtatacgaa ataaacaaaa aaatcttaag taaatttcta gatacctaat ttaatcctaa
     7381 ctagtgtatc ctaaagttaa taacgagttt atcacaatga aaattataaa atgctcccat
     7441 attagatata acatgcaata tttgaattag aactacaaaa tttattcgtt ttataaagta
     7501 aataatgttg agatactaag gaatagaata ttataaggaa ttgagctgaa tgtattggat
     7561 aaaaaaagaa taactttatt agagatggac tataaaaatg ctcgtaattt tcttctgaag
     7621 tctgaaagct atttgaatgc tcagctacca gaatatatta gttttgacga aactttaaaa
     7681 tctgctaaaa aagttttaac ttcgccgttt gtataattaa gcgagacaaa taaaaaagcc
     7741 atttatgtta cactcaaatt gtaatacccg ctaaagcata caaggagagt gaacataaat
     7801 gacttatacc catcttacac caaacgagct tgtaatgata gaagcatatt ttcatcaaga
     7861 aactccggtt gctatcgttg cgaagcagct taaacgtgga cgccaaacaa tttacaatgt
     7921 ctataacttt ctcaaatgtg gtggaacagc acttgaatac tttgaacaat acaaagaaaa
     7981 taagcgacgt tgtgggagaa ccgaaatcat ttttcctgct gaggaaaaag aatacattga
     8041 aaaaagatca actgaaggtt ggaccccaga cgtcattatt ggccgtgcag agcgaacctt
     8101 ctcttgttca ataagtaccc tctatcgcc
//
LOCUS       DAFBQH010000096         8002 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.099, whole genome shotgun sequence.
ACCESSION   DAFBQH010000096 DAFBQH010000000
VERSION     DAFBQH010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.099"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            48..1685
                     /locus_tag="LK694_002506"
     CDS             48..1685
                     /locus_tag="LK694_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053330.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1830..2570)
                     /gene="truA"
                     /locus_tag="LK694_002507"
     CDS             complement(1830..2570)
                     /gene="truA"
                     /locus_tag="LK694_002507"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBK5053331.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2761..3396)
                     /locus_tag="LK694_002508"
     CDS             complement(2761..3396)
                     /locus_tag="LK694_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBK5053332.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3543..4784)
                     /gene="sstT"
                     /locus_tag="LK694_002509"
     CDS             complement(3543..4784)
                     /gene="sstT"
                     /locus_tag="LK694_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBK5053333.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4982..5978
                     /locus_tag="LK694_002510"
                     /pseudo
     CDS             4982..5978
                     /locus_tag="LK694_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
     gene            5994..>8002
                     /locus_tag="LK694_002511"
     CDS             5994..>8002
                     /locus_tag="LK694_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBK5053334.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2625 a         1495 c         1240 g         2642 t
ORIGIN      
        1 atactagaaa agaatcaatt tttattactt ttaaatagga gaaaaaaatg aaaaaaaaca
       61 aaaaccaaat aatagatatc atttttatgt tatttctaag ttttgcttac gtcatccctc
      121 tattcatgtc tatgggaatc tatcatagtg tcaatcagga tacttacttt catttatctc
      181 gaatcatagg actagataat gtatggagtt ctccagtaaa cttcaataac ttcgatcatc
      241 atggaacaat gatgaatatt ttttatccat ggcttactct ttatccagct tttttatttt
      301 ataaaatgat gggaaatctt gttttaagtt ataatatcta ctattttttt atcacttttt
      361 taacaatggt cgtttcttat ttttctatga aacaaataaa aaacaatcgt tatatttcgc
      421 tgttattttc gattatttat acattttccg cctatcgtgc cattgacata ttccatcgtg
      481 cttcattggg agaagctgtc gctttaacat ttttgccttt gatactaatg gggtgttacg
      541 aaatttatat tagagattac caaaaatggt actggttgtc tattggcatg acgctagtag
      601 tgtatactca tttactttct gtcgctatgg tttctgtatt tattggagga acgttgtttc
      661 tttcttttta tttttgggac caaaaaatcg ctagactctt aagtctttta aaagctaccg
      721 tcctcacctt ttttttaagt gcgggattct taattccttt tattcaacaa tctagagcac
      781 aagaattgaa agtcccactg ggaaaagaat taagtggtat ggctcctagt gatatgctga
      841 cacacatttt aaataataac tataacaact atactattgg attattctta tttctcggat
      901 tgattggtgc ttttattttt ataaaaaaat taactgctga tgatttattt attttctttc
      961 ttggtgtatt tgttcttttt tgttcaacca atttatttcc ttggcaactt tttaaccata
     1021 caccagttaa gagtctacaa tttgtctggc ggttaaatgg tttttcttct ttatttatag
     1081 cctatacgat gagtatagtg atctattata ttttttctac taaaaataat tcatggaaaa
     1141 taatggtctt tacgtttctt gcactaatat tgcatctctc aggagtaagc aattctatcc
     1201 atgctaacaa agattcatta acacttatag cacctgaaga cgctgttgct atagctgaaa
     1261 attacaatca tactgattat gcaaacaaag agtcaatcta tcatcctgat atgattaata
     1321 atgatcaata tttactaggt aaccaggaaa taaatccaac aactcatttt atgagtaaca
     1381 aacttacaat tgaattagat aattcgaata ataaaaatac tgtcttgacg acacctattt
     1441 atcgttataa agggcaagtg gcttctatta atggcaaact agtacaaaca aaattatcta
     1501 agtttggcac aactgaatta actattcctc caggtataaa taaagtcgtt attacttatc
     1561 agtacacaaa actagcaatt gcttcaagat atttatctat cgttactttg attttatttt
     1621 tactttatcg atttactttt tctaaacaaa aacaaccgag aagagaagta caacattctc
     1681 actagatttt tatttaagaa agacttttta gctgtacaca taaacaggct aaaaagtctt
     1741 tttttatatt tctttaattt gcttcataca ttatttaaca tcgatattta tactgtgaaa
     1801 catgcgtttc atccattatc aaagtatttt tatcgataat aaacttcatc caaaaacaga
     1861 ccttgtgcag ggacggtttc tcctgcatgt tgccgcactt tgctttcaaa tatttcatcg
     1921 attgtttcta tcggcatggc acctgtgccg atttctagta atgtccccat gataatccgc
     1981 accattttat ataagaatcc gtctccgaca aaagtaaaat gaagcatttc tccctttcgt
     2041 tcgatagata attcatcaat cgtacgcact gtggattttt ttgatttttt caatgcagaa
     2101 aaaccaatga aatcatgtgt acccactaat ttttcgcaag ctgcattcag cttgtccata
     2161 tccaactctt gagggaaata aaagctgtgg tttcgctcaa atgctgaagg aatcacgttg
     2221 ttccaaacat aatagctgta ttttttgccc accgcattat atcttgaatg gaagcgctca
     2281 ggcatttctt cgattctttt gatgacaata tcccgcggaa ggtaacgatt cataaagtcg
     2341 atcatttctg ccagtccaat ttctgtgttt gttttaaaat ttgctacttg tcctcgcgca
     2401 tgggtgccag catctgttct tcctgatcca ataatttcta tctcttcatt tgtcatctga
     2461 tgcaatactt tttcgatttt tccttgaatg gttttatcgt catctccaag tctttgccat
     2521 cctgaatatc tttttccgtc atattcgatt gtcaacttga tgtttctcat attttagtgg
     2581 ctcctctgtc ttccatttgg tcttctctct acctttttaa gattgtagca caaaatatag
     2641 ctcctttctt ctctaaaaca gaagctatac aagaaaactg tggaaatata tatttcagaa
     2701 aaacaaaaaa cagccaccgg atgaagtatc cgctgactgt tcaatttgac ggctcaagtt
     2761 ttactgtttg agatgcaaga tactcaacca ggcaatgggc acataattaa agaaaaaact
     2821 gattctttgg caaaaaccta ttttattcag taatggccaa gaatttatag aaggaattct
     2881 tgccagtcca taaatcgccg caaaaagcaa actgatcaca gacagtccaa ggtataagtg
     2941 gcttacgtcc atttgcccat tttttcgata gatcaagaaa agaagaactg gaaacaataa
     3001 aaaaccactg taacctaaac ctgatccgat attatggacc catgtcgaga cattccacga
     3061 agattcatgg gtattgatac tgaacaaacc tgtaaagata caatcaccta taccatagaa
     3121 cgcgatggct gctgtaacta gggtagctaa cacacgagat gtacttacaa atgcagcata
     3181 caatgcaggc aatgacagga tgaaaaagat ccctgaaacc acagaccaaa taagaaacgc
     3241 ttttcttaca ggactggaaa cctctccaaa tacactgatc acgctcttca tttgatctaa
     3301 ttcaggataa tacaatccta atacgtacgg agtcaaaaaa tcactcagca cgcctaataa
     3361 taaaaagaaa aaaccaaatt tacgtaaaaa atccatgata cgcctccaat aacaattacc
     3421 tatgctttta attgttatta taagcgactt tatcttgatt taacagaaac ctctatttac
     3481 acgaacatct atataactca ttaaaaactc gaatcatttt aaaaatacta aatatctaac
     3541 gttcaacgtt cacttaatcc atctaaacaa tctttcaatt tgattttttc cccacttctt
     3601 ttacgcgcgc ctagctctcc tattgcagta aacaacagat cacttgacga atttaaggca
     3661 gtttccattg aatcctggat aacaccgata ataaatccaa taccgacgat ttgcatcgcg
     3721 acatcattag agataccaaa caaactacat gctaatggaa tcaataacag tgaaccgccc
     3781 gcaacacctg aagctccaca agcagctaaa gcagacaaaa tacataaaag gactttcaaa
     3841 acaaaaggtg cttcaatccc taaagaatgt acagcagtca acgtcatcaa ggaaatggta
     3901 atcgcggcac cgcccatatt gatcgtagca cctaacggga tcgatatgga ataagactct
     3961 tttgtcaatt tcaatctttc agccaacatc atgtttatcg ggatatttgc ggcagaactt
     4021 cgggtaaaga aagcgggaag tgcgctttct ttcaaacaaa agaaaatcaa tggataggga
     4081 ttttctttaa taaacaaaaa cgtgaggaat gggtacacaa ctaatgcgac aaacagcatc
     4141 gtaccgatca aaacaagtaa aagctgcaga tactctgcca atccagctat tccagtcgta
     4201 gcaattgatt gataaaccaa tcctaaaatc cctatcggag caaatgcaat aatcacttgt
     4261 acgacttgtg acaacgctat agatatctga tccaccaact cttttgtcga ttctgaacgt
     4321 gctcgaaaag ctagtcccaa caatacagcc cagaataaga cgccaaggta attcgcatca
     4381 ataagagatt gaacaggatt ttgtgtgaca ttcgtcaaca tagtagataa aacactttcc
     4441 aaactttgcg gtgctttttc agatactgtt tcttgtaaag taataggaac agtaaaaatt
     4501 gaacttacaa taactgctac aagtgcagaa agcaatgttc ccactaaata taagatcaaa
     4561 ataggcttca cgaatgtctt attcccaatt ttatgcttgg caattgacgc catcgtcaag
     4621 aaaaaaacca gaataggtgc gattgccttc aagcaactga caaacaacgt tcccaatatt
     4681 gaaataaatg tccatgtggg gaaaaagata ccaaacaata ttccgactaa aataccaaca
     4741 acaatacgtt gaactaacga catatccaaa aacttctcta acaaatgacc accctttcac
     4801 aattttaaca atcattgtat cattttatgc aaagaaacta aaggtataat tttctaaaaa
     4861 ttctaacaat acaaacaacc atttcttgct aattttgtac tttttctgtc tacacgttgt
     4921 tttttgagat attctaagtt aggatgttat taatgggcaa cttatcagga aacgttttct
     4981 tatgtttttg tccaattttt ttgtgaggtg tttattcttg aaaaaaataa agaaggagag
     5041 ttatggcttc ctgacaatct ttgtcgcttg tataatgatg gttttacagc gtatattgat
     5101 ttcagcagtc actattccta catatgcaac aaaccctggt cgattctaca tctatacatt
     5161 tttacaaact tccgttgtta ttggtgcaaa tttgatccca ctttacatgg gttaccaata
     5221 tcaaaaaatc gcccgtttaa aagttttaca ttatttaagt cgttttgcct ttatattcct
     5281 aatagccaca ctagtttgca atatcttttt tttacgtaca agctggcagc ttgaatattc
     5341 gtgattattg gcttatccta acacctatca gtcagaatta ctttacttat gctgtttctt
     5401 gtactttgct tttttgtagt attccttata cagttggatt tttaaataaa ttatcgaaaa
     5461 caacaattaa atatctacta ttatttttaa caattggatt agttggctgc tctactctat
     5521 ttaataaaga cccgtttgca ttacagaatg gacagagtat tttatggatt ttttatttat
     5581 ttttaatggg ttatggtttg aaagaatgga actggaaaga aaaggtccgc tataagcttc
     5641 ctcaagtatt tatcagcttg attgttttat tcggtttgat tattttgatg acacaaattt
     5701 cactcatcat cagaggaaat acagatacag cgctgaggtt ttctaaacca tattctctct
     5761 tcgcaatgta ttatactttc tcttcgtttt tatttttaga aatgctatct gataaattag
     5821 gtataaaagg aagaggaaat tttcttgctg tatttttgat tgtcactcaa gttatatgta
     5881 atttcccttt agtttcttat cgcgtatcta catttatgaa acgtgaattt ccaaactcag
     5941 ggaaggcatg gctgataaat atcgggcagt tttttatttg ctatttatta gcagtgtccc
     6001 tcctagttat ctttttattt tatatacaaa aaatccgttt cattcatcgt ttcatcgaat
     6061 acttcgcttt tgattcgatt gaacaattaa ctcagcgttg gaaaaaaaga attcattggc
     6121 tttttgttca taaaagttat tttttagttg cagcattttt ttactgcttt accttcgtgc
     6181 agattttcgt tttagaacca aaagaaggtt ggaaagaaac tatccaggtt gccttaaaaa
     6241 tatttactca gcgacaagca cccatgatcc tgacaatcat cattattatg ggatttttct
     6301 tcttactatt gttgatttcc aatcgttttt ggtacgcctt gactgctacc ttgatcatta
     6361 acttactgct cactatttcg acagtaatca aaatggaaat gcgggaagag cctgtctttc
     6421 cttcagattt aaaaatactg accggattgt cagaactatt atcgatggtt agcccagtct
     6481 tattaatcgt gggcggattg atccttctcc tattattgat tactagtatt attgtacaac
     6541 gtagactaca aaaacagtat tccttgaaga ttcattggaa acgtcgattt atcagtatcg
     6601 ctgtattatt aggatgtttt tcgggtgtct ttttcattaa tcacaaaaat tcgccttctt
     6661 tcttgctgtt taatttattt aaagtaaaca agactttctt taaccagcaa ggcgccgtaa
     6721 aagataacgg accagtcatt caattcctaa acaatatcga cattaaaatc atggaagaac
     6781 ctgctggcta ttcgaaagaa aaaattaatg gaataatgaa aaagtacaac acaaaggcag
     6841 atgaaatcaa ccaaacaaga agtgactggt taaacaatca gacgatcgtt ctcaatttaa
     6901 gtgaaagctt cagtgatcct gcacgtgtcc ctaatctgac agtacctaca gaccctattc
     6961 cttacatcaa aagcattata acagaaggaa caggaggatt gatgttatcc gtcggttatg
     7021 gtggcggaac agctaacatg gaatgggaag gactgacagg cttgagtatc agtaacctgt
     7081 ctgcttcgtt agttacacct tatacccagc tagtggagcg tcagaaaata agtccaaata
     7141 ttaccgatct attcgatgaa aaaatagcca tccatccttt tacagctgct ctttatcgca
     7201 gaaaacaagt gtttgataaa tttggttttg acaggttcta ttatttagac agtccggata
     7261 aattaactta tacggataaa atagaacaaa gcccacgcat atcggatgat tcagcataca
     7321 aagaaacctt gaaagcttta caaagcaata cagaaacgtc acaattcatc caattatcca
     7381 ctatgcaaaa ccatatgcct tatgatgatt actatagtga attgaactac actgctgaag
     7441 gaactgctgt cattcctaat agaagaaaag aattacaaac ttatatgcaa ggccttcact
     7501 ataccgatac agcagtaaaa aaattcatcc aagaaatcga caaaatcgat aaaccgatta
     7561 cctttgtttt ttatggtgac catttacccg caatctatag tgggaatgat tcgaaaaaat
     7621 atggattaga acagcaccag acagattatt tcatttataa caacaagtat agtcgtgaac
     7681 aagcagctac gttaaataaa gaagttgttg ctcctaataa ctttggtgct atggctcttg
     7741 agcatgcgaa tatcaaagta actccttact atgcgttgtt gacagaagta tctaatcatc
     7801 tacctgcatt aacaattgat cctacagaaa gcttgtctaa tcgttttaat ggaaaacagg
     7861 tctttgttac agaggatagt caaattatcc aagaaaaaaa tttaactgag aaacaaaaag
     7921 aaattctaga agattatcgt ctgatacaat atgatcttgt tgcaggtaac cagtacagtg
     7981 caacatgggc gacacaaaaa at
//
LOCUS       DAFBQH010000097         7933 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.101, whole genome shotgun sequence.
ACCESSION   DAFBQH010000097 DAFBQH010000000
VERSION     DAFBQH010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.101"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            181..1242
                     /locus_tag="LK694_002512"
     CDS             181..1242
                     /locus_tag="LK694_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK5053335.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            1560..2150
                     /locus_tag="LK694_002513"
     CDS             1560..2150
                     /locus_tag="LK694_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HBK5053336.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            2177..3487
                     /locus_tag="LK694_002514"
     CDS             2177..3487
                     /locus_tag="LK694_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBK5053337.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            3484..4203
                     /locus_tag="LK694_002515"
     CDS             3484..4203
                     /locus_tag="LK694_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBK5053338.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            4218..5303
                     /locus_tag="LK694_002516"
     CDS             4218..5303
                     /locus_tag="LK694_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK5053339.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            5439..6971
                     /locus_tag="LK694_002517"
     CDS             5439..6971
                     /locus_tag="LK694_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK5053340.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            7149..7370
                     /locus_tag="LK694_002518"
     CDS             7149..7370
                     /locus_tag="LK694_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBK5053341.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT         2819 a          846 c         1510 g         2758 t
ORIGIN      
        1 catacttacg aggatcatat tattgattta ataaataatt taaaggaaat aatttttcta
       61 gaagaagata gtatgtcatt cacatcatca agatcagagt ctattttttt cagagaattt
      121 aagaaaatcg ttgatgccgt cattgactaa ttgagaaaac gtgagtgaaa ggatagaagg
      181 atgaattcga gtgatctaat tagtgtaatc atacctgtat ataatgttga aaagtatgta
      241 ggaagttgtt tggaatcagt gtgtaatcaa acatataaga acctagatat tataattgtg
      301 gatgatggat ctaaggattc atctaataaa atatgtgaag actatgcatt aaaggattca
      361 aggattcgta ttattcataa aaataatgct ggcttgggat tggcaagaaa tacaggactt
      421 gaacatgtaa aaggaaaata tgttgctttt attgattctg atgattttgt tgcagctaat
      481 tatattgaag caatgttaaa tggtataaaa aggagtaatg cagacacatg ttatgctgga
      541 tattatgaat attatgatga taataatatt atttcaaagc cagcaagata tgatagtacg
      601 atatttacaa ataacgctat aattgaaaaa gtgttgcttg gaatgattgg cactgagccg
      661 agttataaag tcgattttat attgccaatg tcagtttggc atgcgctgta ttcatctgag
      721 ataattcaac agcacaatat caagtttccg tcagaaaggg attatatatc tgaagatatc
      781 atattccata tttcatattt gcaacattca aaaataacaa gttttattaa agattgtatc
      841 tattattata gatgtaataa taataattca ttgacacata attttagaga taatgagttc
      901 gattgtaata ttgaattagt taaaaaaatt gaaggcgatt taagctatat attagaaaat
      961 gagatatttg taaacaaaac aaggagactt ttgttgggac gctgtcgtac ttgtattcaa
     1021 aaggcagtta gatatgctag ttttaataaa gaatttgatt taaactccta tttgtctaag
     1081 atacttatga acgatgagtt gattgcctgt attcactcat acccatataa acaaaatcca
     1141 attaaacaac gaatatttaa ctatttttta ctaaaccaat ccattattgg ggttaaaaca
     1201 ttagcatatt taagcggaat gggtagaaat aggagatttt aatcataaaa aactgtacta
     1261 tagtgaattt ttggagattt atattccagt tatatattaa tgattgtgat acttgtacta
     1321 ttgtaaaaat tcgttaggtt taaataactg gaaatatcgg aaaatgggca ttatacatca
     1381 cggacatccc ttcttttgga aaaaatgtta cgatttttat aagtaatatt gtatccggtg
     1441 gaatcaacga tgaagataac atatccatcg ttgtagattc agtagcattg aatgatgctc
     1501 ttgttgcaag aaatccgaat aaaattgtta agtataaatt gaattaatag gagatatgaa
     1561 tggaaattag cagtaaagaa tatttaaaag cggttcttcg tatggaaaag aatctatata
     1621 tagaatctaa aggacaacac acaaaattac taaaaataat gaaatcacat ccggactaca
     1681 acgcttggcg gtatactcgt cgaatgagaa ttacatctta ttattattca aagagaaaga
     1741 aaaatatcat ttatgcgatt atgtatattg tgtcttgcag aagaatgaat agtttgggaa
     1801 gaaaatgtgg tatagagtgt ggtgaaaatg tatttgatga aggtgtaaga ttttaccata
     1861 cacaaggaat tgttattaat ggtaacgcca taataggaaa aaattgttac ttatatggga
     1921 ataactgtat tggaaatgat ggaatcgatc ctaagtgtcc tgtaattgga aataatgtac
     1981 gtgtatgcgt gggtgcaaag attattggta atgtaaaaat agctaacaat gtagttgttg
     2041 cggcaggagc tattgttatc aaagattgtc tagagaatgg tgcaattctt gcaggtgtac
     2101 cggcaaaaat aatagggtat gttaatttaa caaaagtatt tttagaataa tttttagcga
     2161 aagaaggtga ataacgatgc gtatttcaat aaaaagatgg tcaggagcag attggacatt
     2221 gttcacgttg gcattttttg caatctccat agttcttgta tatactttta acgccccaag
     2281 agcaatttta tatttgggag atataattaa tatctttgtg ttattaaatg cacttagctt
     2341 tagacgcaga aaaggaatat atgctagcaa aaatgagatg ctgatagttt tgtttttact
     2401 agtttctatt tgtagctcag ttattaattt agaaaatata ggattgatta tctgggggat
     2461 acgaaataac actcgtatgt tcatagtata tataaattgt gcaacatatt taacaattaa
     2521 agattactat aaagtattcg ctataattga aaaattattc tggatttcac tgccactttg
     2581 tttaattgaa agattcatgg tcacctatcc aactggaaca attgtaggag atatgatagg
     2641 tggagttttt tggaattatt caggttgtaa tatgccattg aatttaattt tgtgtgtgac
     2701 agttactgtt gttacaatcg gatatttcaa gggagaaaaa aatttcatcc attttatttt
     2761 atgtgtttgt gcagcacttt ttatgtctgc attagctgaa ttgaaagttt ttattcctga
     2821 attatttata atatttgttg caatagtatt cactttaaaa gttgatttta aagtagtgat
     2881 caagatatta tcatgcgtaa tacttgtgtc gatagtatta tcctttttta taacaatatt
     2941 tattacattg aacgataatg gacaaagcta tgctgataat ttcacaattc aaggtctttt
     3001 agaatatgcg tcaagagatt ctggatatga tggtgttggt gatttaaatc gaataactgg
     3061 tatctctaga gtagtagatg aaatatttaa taataatttt agattagtcc taatgggagt
     3121 aggcattggc aatgcagagt ttaccaattt ttttgtcagt gagttttata aatcgtttgg
     3181 taatcttcat tatcagtatt ttcatcatat ttggttattt atagaagttg gaatattagg
     3241 agtttcgata ttcctatttt ttataataaa gacatttttc aatgcaaaaa gatcttttat
     3301 gaaggataaa gatgcaagtc ttgttaaagt tattgcaatt atgtcggtat tcttttttgt
     3361 gtataacaca acacttaggg gtgaaacaac ggggccactg atttatatga ttttagccat
     3421 gcctgaagtt gtgaaaagga aagagcatta ttatagtgaa aaaaatacaa aaggagagat
     3481 attatgattc ctaagaagat acattactgt tggttcgggg gaaatccact tccgaaaaaa
     3541 gatagaaagt gcatagaaag ctggaaaaag tattgccctg attatgagat tattgaatgg
     3601 aatgaatcaa attatgattt aaataagaac cgttatatgg ccgatgcata taaagaacgt
     3661 aaatggggtt ttgttccaga ttatgcaaga tttgatatta tttataatca tggtggattt
     3721 tatttagata cagatgttga gctaattaag tcgttagata tacttcgtga aaatcatgcc
     3781 tatatgggat ttgaagggga aatctgggtt aatggcggta ttggttttgg tgcagaagaa
     3841 ggacattctg ttattaaagg tttaagggat atttacgatt cattaagttt ttatcaaaat
     3901 gatggtacat tgaacttgaa accgagtccg tattatatta cagattatct tgaatctatt
     3961 ggattggttc gcgataacag cgaacaaata ataaatggcc tgcatatcta tcctacggat
     4021 tattttgctg ccaaggatta tgatactgga aaaatttata aaactagtaa tacaataagc
     4081 attcatcaat ataatgcatc ttggaaaaca cctaaacaaa aatttgttta tagattaaaa
     4141 tcaacaattg gacaaaaaaa atttaacagg ttacttgcac tgaaaaaatt tttacactta
     4201 taaaggagaa aagtactttg aagaaaattt gtttcattag tggtgtaatt tctcgttctg
     4261 ggggaactga aagagtaggg agtattattg ccaatagctt gtctgattta ggttatgatg
     4321 tcactatttt aagcttttgg aatagtggag agccttattt tgggttagat aagacgatta
     4381 aggttgatta tatgttaaat cctaaaacgg agggaaaatt atatagaact tatttatatc
     4441 cggtttggaa attgcacaag tatatatgca aaaatgaatt tgatatagta attgatattg
     4501 atactttgtt gtctttgtat actacaaggg caattaaagg aacaaagtgc aaattgattt
     4561 cttgggaaca ttttaattac tggacaatgg aaatgctgaa agaaaagaag agattcaagg
     4621 ctaaaaaatt gataaaaaaa tatgcaaaga ggcttgtggt tctcacagaa gaagatcgtc
     4681 aaaaacatat tgatgaatat agtttatcag aagattttgt tataactatg ccaaatccgt
     4741 gtatatctaa tatcgagtac gaatatcatt atgataaaaa gagatttctt gcagtagggc
     4801 gattagtgga agagaaaaat tttgctgaca tgataagtgc ttggaaaatt gtagaaaaaa
     4861 aagtagatga ttgggatctt atcattgtag gaaaaggtga attagaaaaa gatcttcgcc
     4921 agcagataaa agatttaaat ctgcaaagag taactatagc agggcattca gaccatgtgg
     4981 aggaatatta taaatctgca tcttgttatg tgcttagttc tgtttatgaa ggatttccta
     5041 tggttatatt agaagcacag tcttatggat taccagttat ttcttatgat tgtaaaactg
     5101 gtccgagaga tttggtacat cataatttta atgggatgtt ggttgaagat aagaatatag
     5161 accaattagc taagagcatg ataatgttta ctaagaatac tgatttagca atgagaatgt
     5221 cactgaacgc atataataat gtacaaaaat ttaatttgaa agaaattaca aaacaatggg
     5281 ttgctttgat tgaaaatgta tagttttttg tggttgtcca aacatgaaaa ttagcgtaat
     5341 taatcaaaga gctagaagaa acctattaca tttcaagatt ttagaaatta tatagtatta
     5401 ggtaacaata aaaaatagaa agtttggagc catatgatat gataattaat cgtaaacaaa
     5461 atgctaaaag gaatataatt actggaacaa tttttaagtt atatcagatt atcattccct
     5521 tttttatgcg tacacttatg atttatcaga tgggaatgga gtatgctgga ttgaacaacc
     5581 tgttcacatc tatatttcaa atgttaaatt tagcggaatt gggaataggt gctgcactaa
     5641 ctgtaagtat gtataaacct atagcagaag acaatagaaa agagatttgt gctttactga
     5701 acgcatataa aaagtacttt tcaattattg gatggatagt gtttacatta ggattgttat
     5761 gcattccttt tttgagattt ttagttaaag gttcgattcc tggaaattta aatatgacag
     5821 ttttgtattt aatgtatttg ataaatactg ttttgtcgta ttggttattt tcatatagaa
     5881 agagtctaat atatgcacat caaagaaatg atgtaattaa cgtgtctatg ctaattactt
     5941 ttacattcca atttgtaatg caggcatatg ttctgatttt cttaaaaaat tactatttgt
     6001 atttattggc ttctattttg ggacaaatag ttcttaactt aatgtgtgct gttgtttctc
     6061 aaaagatgta tcctgattat actcctgttg gtgagttaga gaaagaaact attgacgata
     6121 ttataaaaaa agtaaaagga ttagttacaa acaaggtagg cggtacaata ttacgatctt
     6181 cagattcaat tattatttca tctttcttag gtctttcagt acttgcgatt tatcagaatt
     6241 attactttat tatttctgcc ataatgagta tatttgcaat aatttatgaa tcatgtatag
     6301 caggtatagg taacagtctt attgtagaaa gtcaagaaaa aaattatata gatttttgtg
     6361 atattacaac aattataggt tggtttctgg caattagttg tgcatgcttt attacattat
     6421 ttcagccgtt catgattatc tgggtgggaa gtgataatct tatgagtatt tcatttgtta
     6481 tatttatgat agttcatttt ttcttatatg aaattgatca gttaattggg ttatacaaag
     6541 atgctgctgg aatatggtac gcagacaggt ttagaccatt aattacagca atgataaaca
     6601 ttgtattaaa tatcatcctc gttaattggt tcggattata tggagttttg gcggcaacca
     6661 ttgtttcagt attgttggtt gatttaccct ggttattata taatgttttc caaaatatat
     6721 ttaaatttga atcggcaggt aaatatgttt ggaagctcat aagtttgtgt gtaatagctt
     6781 ttgttgcttc ttatttaagt tattttgctt gttcgtatgt taatatcgga aatttcaaag
     6841 tgataagttt agtgataaat ttagttatat cggttgtcat atcgatttta gttattcttc
     6901 tgtttaatat ccgtaataag aagtttaagt atattttgaa agttcttacg tcggtattgg
     6961 taaaaagatg agaagagaca tcagaatgat atataaagta actacaattt ttaggctgcc
     7021 gaataggcga acgatgtata gtttttgttc caacgaagac tcttgttgaa attggagatg
     7081 atatgaagga gttacaattt tgactcatgt ttatgattga tcggttttaa aagtagtatg
     7141 gagaagcttt gggatcatcg gagaggatca aaataggtaa taatgttttt ataggaatga
     7201 agtctagaat actaaagggt gtacatatag gaaataatgt agttataggt gcaaatagtt
     7261 tagtaaacaa ggacgtatca gacgattgtg ttgttgcagg aaatctagca aaagtaatta
     7321 tgtccctaga tgaatattat aaaaagataa acaatatgaa gaatccgtaa aacttgtgaa
     7381 actataccga gaataatatg ggaaaaatat tgatgaaaaa gcattatatg agtttttagg
     7441 gctgtttaca gatggagatg acaatttgtc gaagagttgg gagcaatgca aaatttagta
     7501 ggggattata aacagactga caaggtattt aaagaacatg cacaagtatt tcataatatt
     7561 gaagaatttt tgaatactgt gagataggag ttgctacatt gagaaaagta tgtatttttc
     7621 caatgtaaaa gattacgagt ttgaaaacga caccataata ttatttttta agaagatatt
     7681 atagagctta ggaacggaag tttatttaag gatgttgaat aaaatgaaaa ataagattag
     7741 gtggatgaaa aaggcagatg attttttact ataattaaaa atattgttac tatgttatca
     7801 atcgtataaa aatacttact cgttatcaag acgattattt cgaatatttt aggaaaaata
     7861 aattgaatat tagagaatgt gataatacac tctaatatga tcatgtgtaa ttggaagcga
     7921 taagtgtata ctt
//
LOCUS       DAFBQH010000098         7908 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.102, whole genome shotgun sequence.
ACCESSION   DAFBQH010000098 DAFBQH010000000
VERSION     DAFBQH010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.102"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..949)
                     /locus_tag="LK694_002519"
     CDS             complement(<1..949)
                     /locus_tag="LK694_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5053342.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKI"
     gene            complement(956..1786)
                     /locus_tag="LK694_002520"
     CDS             complement(956..1786)
                     /locus_tag="LK694_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5053343.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(1783..2598)
                     /locus_tag="LK694_002521"
     CDS             complement(1783..2598)
                     /locus_tag="LK694_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBK5053344.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(2595..3419)
                     /locus_tag="LK694_002522"
     CDS             complement(2595..3419)
                     /locus_tag="LK694_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBK5053345.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(3409..4509)
                     /locus_tag="LK694_002523"
     CDS             complement(3409..4509)
                     /locus_tag="LK694_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK5053346.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4687..5472
                     /locus_tag="LK694_002524"
     CDS             4687..5472
                     /locus_tag="LK694_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HBK5053347.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            5505..5888
                     /locus_tag="LK694_002525"
     CDS             5505..5888
                     /locus_tag="LK694_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBK5053348.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            5964..6095
                     /locus_tag="LK694_002526"
     CDS             5964..6095
                     /locus_tag="LK694_002526"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK5053349.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            6064..6300
                     /locus_tag="LK694_002527"
     CDS             6064..6300
                     /locus_tag="LK694_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK5053350.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(6378..7349)
                     /locus_tag="LK694_002528"
     CDS             complement(6378..7349)
                     /locus_tag="LK694_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBK5053351.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         2296 a         1861 c         1546 g         2205 t
ORIGIN      
        1 taattttctc ttcccgatcg ttcaaccgat ccactaaaat ttctttgccc tccagcgttt
       61 gaaaaacata ccgttgaatg ttgcctaaaa taaatgattc cttccataga accggaatct
      121 taaccccctg aggaaccggt tcaaattgaa tcagctccgg tcttaaataa taaatgcagc
      181 gattgccctt agcggaaaac tcgtgggtat actgattcag ctcataccat tcgaaacgat
      241 tatagttgcc gataaattcc gcaacaaatc ggctctgagg attctgatac aattccttcg
      301 aggtggaaac ctgagccacc cgcccagctt ccatcacaaa aatccgatca ctgattcgca
      361 tcgcctcttc ttggtcatga gtcacaaaaa tcatcgtcat ccctagcgat tgctggatag
      421 cgcgcaaatc ccgctgcagc tgttttctaa ttcgggcatc caaagcactt aatggctcat
      481 ccagcagcag gactttcggt tcagtgacca atgagcgagc cagtgccacc cgttgtcgtt
      541 gaccgccaga aagaaactgg acatttttat ctgcctgctc cgtcatatcc accaatgata
      601 gcatctcaaa aaccttttcc tgaatcacct caggactcag cttctttacc ttcaaaccaa
      661 aggctacatt ttcaaaaaca gtcatagttg gaaacaatgc atactgttga aaaacaaagc
      721 cgatattgcg atccttcgta ggcttatcgg caatattctc attgtccagc acaatctcac
      781 cgctctttgg ttgttccaat cccgtgatac agcgcagcaa cgtagacttt ccgcacccgc
      841 tgggacctag taaagtaacc agctcaccct tttcaatctg aatttcatca atagctaaaa
      901 tctgtttttc atcaaaaaac agattgagat catgtatttc taaaaacatt ttccgtcaca
      961 actcctttaa gggcttcttt ttcgactgct tctcttccct cgtctgctta agggacagct
     1021 gttgcttttg tcgataagtc agcataaagg tagttaaagc aattcccacc ataaacagca
     1081 aatacaaaac catcacagcg ctagccaaat ggccgttttc gttcatccgc cgcagcatat
     1141 agatgcgtac cgtctgaaat cgaccgccaa tcaacaggtt cgtcagcatg tactccccaa
     1201 aaaccgaaga aaaaatcatc agagaaacta gggtcacacc gatacggatg tttggaaaca
     1261 gcacctggat aataatcgta gacatcgggg cgcccaaggt ttctgctgca tccactaatt
     1321 ccttcagatt aattaagcgc atctggttgt taagactaag atagacaatc ggcaacgccg
     1381 taataaacaa tgcacctacc aacactacaa acattgggat accagtttca gcatacgttc
     1441 gaagcaaagc cgttaccaaa atcactcctg gtaaagcata cggcagcagg agcagctttt
     1501 ccagccaacg attcaaacga gggaaataca aatgaatcca aataatggtg ggaatcatca
     1561 acaacagaat cgtcaccaag accacacctg ctaataaaag tgagcgaccc accgcagcaa
     1621 taaattccct gtccgtcagc agctgttgga accattgcaa ggtaaaatca ctaggtaaaa
     1681 gcgattttga ccatttagtg gagctggcat acaaaatcgt tccaataatc ggcagcaagt
     1741 aatacaggac aaagcagaac aaaatcggtt ttattaactt tttcacgtct gcaccacctt
     1801 gcctttcttt tgaataatca acattgccgt taccagcagg acacctaaag cgactgccaa
     1861 cgcactgcct aaattaggct tggcgaacac atctcccgac actaaagccg caattcgaat
     1921 cgttaaaaga tttacattac tgctggtcag ggcatacgct gtttcatagg tccccatgcc
     1981 attagcaaat aagatgacaa aggtgctggc aatcgatggt cgcaaaaaag gcaaagccac
     2041 cttgaaccaa gcatacgctg atgaagcgcc cagcaattgt gcggactcca gccattcctt
     2101 tttcacttcc ttcatgctgg gatacaaaaa caagacccct aacggaattt ggaaataggt
     2161 atacgtgatg gtcaatcccc accaagaata caaattgaag tccgctagaa atggcaaaat
     2221 cattttcagc acacctacat tccccaacaa aataataaaa gagaatgcca gcggaatccc
     2281 agcaaaatta gctgccaaat tggaaacaaa ggtgattttc tcctgcaatt tagtcgatag
     2341 attcattaag cataaacata aaataaacgt cccgattaac ccaatcacac tggagctgac
     2401 agcaatcagt acgctgttaa caaaggcctg tgtgtaatat ttgttgctga aaatatcact
     2461 gtaattggcc aatgtccact ggttagttaa atcactgtgc aagctgcaca gaaccataaa
     2521 aagcagcggc agcagtaaaa atgcacagaa gataaaggag gcaggaacca gaaccaagcg
     2581 ttttttcatc tctttcatcc cctcatcaat tcttgaatgt cctgcatttt ctctgacggt
     2641 gcaattccta ataaccagca aaacaatgga gccgccagca gctgtggtac agtttgttcc
     2701 ttcagctttt ggcgtttatt tgaaatcaaa taaaaaggaa cttcacgatg tgcagccaag
     2761 ctgccgccat gcagtccgtg cgcatccatc ccgtgatccg cagtcaccac gacttgataa
     2821 cctttttgca gccattcagg aaggtaaata gccaataatc tgtccgcctg attaaccgct
     2881 gctgcatatt catgagaatc ggcggtaaat ttgtgaccga catcatcaat attcatcgaa
     2941 tgtacgacca aaaagtctgg ctgatactga ttcactaaat aatgggcatc cgcaaataaa
     3001 tgcgaatcag ggtaagtgtc ctcactataa aagatgccat gctgaatcag tcgctcctcc
     3061 tgatgctgga tgcgatcaac caaacgatta tagggggctt gattgtacaa ctcgctgtac
     3121 cagtggtaag cggcggctcc tgtttttccg ccagcttcct taactaattc aaacagcgac
     3181 cactctttgg atcgctgagc atggtaatta ctatagatgc cattttcata ggctggaacg
     3241 ccggtcatca gtacttcgta taacggacgt gaattgctgg gcagctcact ttttaccttc
     3301 aataagcttg cttgctggtg ctcaactaaa tgattcatga acccaagctg ggcgacggct
     3361 gtgtcgtagc gacagccgtc tagtataact acaatcagtt tgtcaagtct actttgcata
     3421 ggtcagtacc tccgcctgcc aaagctctgg aagctctaca gaggtcttgt tccaagcttc
     3481 attgtcttca actggcaccg ccgcttcata agcatcctcc ggcagtaatt tttcagctac
     3541 ttctttaggc agctctacct tgcgaatagg tcgcgcatag ccctttgcca aattgatttg
     3601 cccttcatca gataaaatat attcccgtgc tagctttgcc gcattgggat gaggggcatt
     3661 tttattgatt aaggttgtat acccgctaat gatggagccg tctgttggca cagttacctc
     3721 aaaacgctcg gagtcaattt ggtcacgata atttagtcca ttgaagtccc aaacaactgc
     3781 cacatcgatt tcaccttttt caagatttgc tacgctggca tcaacggtgg acaaacggcc
     3841 tttttctgcc atttttttgt agtaattcag acctggctga atatcctttt cgtcacctcc
     3901 gttggcaatc gccgcagcca atacagcaaa ttgtgcttgg ttggcaatcg tcacatcccc
     3961 cacgctaact tgatagtcac cctcttgcag ctccttccag ctagtcggtg cttgcttcac
     4021 attctcttta tcggtgataa aggaaatggt tcccgtatag cttaacaacc aatccccatc
     4081 atcatccttt gcccaatccg gaatttcctc ccaatagttg gtcttataag gtaaggttaa
     4141 ctctttgctt tcagccaacg gaccgaagct gataccgaca tcaccaatat cggcggttcc
     4201 attttttcct tctgattcaa acttagccaa ttcttctgca ctggacatat ccgtatccac
     4261 gtgacttaag ccgtattctt tttcaaggtc gttccaagtg cctacccaat ttgcccaagt
     4321 atccggcatt cccaagctgg caatctctcc ttcttccttg gctttttcta taatcgtatc
     4381 taaagagtcg tctgccgcag ccgattgctt cgttgcttca cctgagacgc agcctgtcag
     4441 caggatactt actgttgctg cacaaaataa accgatagtc atgttacgaa ttttttcatt
     4501 cattttcatt cattcttctc ccctttcgat aattccattc taacaaagct ttgtaatgag
     4561 agaatcagtt tcttgaaaag gtttcatgaa caatgtaaag gttgtatatg gtttgtaaat
     4621 ttcgacgaat tcccgttaac cttctaaagc ttttgcggta tgatagtaga aaaaaggagg
     4681 ctccctatgc agtattatga tcagcatctc cacacgtatt tttcctttga ttcagaggaa
     4741 aaatttgaaa actatttagc ctatcagccg gagtttttcg tttctacgga tcactttgat
     4801 ttaaacaatc catctaccgg aaaagatgac attccggatt atccagccta tataaacaaa
     4861 ctggactctt taaaagaaac ctattccacc aaatttctgc gaggtatcga gattggcgtt
     4921 gttccaggac aagaagagcg gatcgtggat tacctcgctc agcatcctta tgatttaaag
     4981 ctggtaagta ttcatcaaaa tggccgcttt gattatatgg acgatgtggt tttaaccaaa
     5041 gatcccattc aaactaccaa ggaatatttt gatcagatgt tgcaggtctt gacggatttt
     5101 ccagaaggag atattttgac tcactttgat tatggattac gccgcttcac cttcacagcg
     5161 gaagaattcg cggagcattt tgaggctcag ctgctaacga ttttcaaaaa ggttgttgaa
     5221 cgagaattgg cgatggagtt aaatgccaag agcttcttga aatacggcaa cgaagacctt
     5281 taccgttatg ccgttccttt gtatcaaagt gtcggcggaa agctctttac cttaggctcg
     5341 gatgctcatg ttgccgaaga ttatcagctg ggctttaaaa aaatgagtca gctgttggca
     5401 gactgtcagg tagaaaagtt gctagtcatt caaggaaaag aacgccgcct gacgccgctg
     5461 ccgattttgt gattcctata taatagaaga aagtggtgaa aaaaatgagc aacctgtggg
     5521 actacaatca agaggcaccc atccattatt taattgcgcg acattgggat gcgttgaaaa
     5581 ttgaggcggt ttgccgatcc ttgttagcag ccgttccaaa acaacaactg gaaaattttt
     5641 tggtggctga cagcttgcag cgggaaaaag ttcaggctta ttttgcagcc tttaaagatc
     5701 agcctttgga atatttgcat gcgcagtttc atctttttta tcaagtagcc gccccagatg
     5761 actacaacga tttacgagga cagctgcaat tgacctttca agcggatgaa accgcctata
     5821 ccgttttatt gggaatggct cgccttggtg atcaggcgaa ggtcgagtgg cggattttcg
     5881 atatttgatg gcaggatcaa ggcgtttcag ttgcaaacct tcctctcttt caattagaaa
     5941 aaccgcagca ggaagaagtt tgtgtgctgg tcaccgatgc tggtgaaatt cgtctccgct
     6001 tatttcctga ccaagctccc ctggctgtag ccaactggcg ctccctagct gagcaaggcg
     6061 tttatgacaa cacacctttc ggctcgtgtc attaaggaat ttgttattca aggtggcgct
     6121 ttggatggtt ctggtgatga agctgtttcc agctatgagg gctttttcgc tgatgaagtg
     6181 catcgcggac tttatcattt taatggcgcc ttggccttag gggaccacgg cccccacacc
     6241 aacggcaacc agttttttat tgtgcaaaac acaaaggcgc aggctgacct gctaatgtaa
     6301 agcaagcata cttggagctt ggaggactac cggagctgga tggtcgttat actgtttttg
     6361 gacaggtata ccaagggtta gaaatcgtag cagcgatcgc agctcagccg acaaacagtg
     6421 aagatgtacc gattgagcca gtgttcattc gacaaattaa cttcgaaaaa acatcgtaaa
     6481 aagacactca ctgacttcag caaaagctct attcttgagc tggctgcagc tagtgagtgt
     6541 ctttcattta tccataagat aaagcttgga ttcaataaat aaataaatgt tttggttaaa
     6601 gctgtgacca gcaaatgagg aaccgtgcga gccagttgat ctgccaatgc aaacatttct
     6661 ttaggtgaag gcgctggcgt gtctgccaac ggatactcca gtcccaatgc ttggtaaccg
     6721 tgcataccat agcagtggta cggtagtaat tctaacggta cgaaccgatc aagatctgcc
     6781 acaaaacaag caatcgtcag cagctcggtc aggttcatat tgatacctgg aaccacagga
     6841 actcgaattc gcacttcgca gttaggatac tcaattaaaa agcgacgaat attcttcaaa
     6901 atttgttggt tatctaagcc ggtccattgt ttgtggagct tgctggtaaa catttttaca
     6961 tccacaaaca atgtatccac atagggagct accttttgga tttcattata agcaccacaa
     7021 aagctggttt ccagtacggt attgattcca atgtatttcg atttttgcaa aatttcttta
     7081 gcaaaatccg cctgaaccag tgcctcgcct ccgctaatgg tcactccgcc atccgagtga
     7141 aagtaaaaaa cagcatcctt ctcaatctca tccattacct cttcagcggt catggtctgg
     7201 ccaaatcctt tttcaatctg cttgctttgt gattcgggaa tcgaacacca ggcacagcgt
     7261 aatgggcagc cctttaaata aacaacggtc cgtaaaccag caccatcatg tagagaaccc
     7321 tgttctattc ttaatactgt tccttccata tagatttttg aattacacac tagtttaaac
     7381 taaaacacac aaggaaaaac ttaccattta atcataaaat tagctcatat aaatatacta
     7441 ggatgtaaaa tagactggtc aaaatctggt caaaaaagag gactaataca ggtagtcctc
     7501 ttttttgtta ttttaatatt ctaaatcatt ttttattggt ttattccctt attcgatgta
     7561 gactcaaatc actatatcac tagatatcaa acggcgtcat acggaatcaa aaacatcaaa
     7621 tttgacctgt taaatggtca ttgaataaca ttcaaaatca ataaaacgga gtcagacacg
     7681 gagtcaaaaa cgctgagagt aaaaaatata tacagccttg taggtaaagt accacagggc
     7741 tgtatatttt ttatttcaga ttaccttaaa tgattccatt tactttttat ctcatttttt
     7801 cctactagtt agtatcaaat actctcctat tttctcaata aactattttt ggatacatgg
     7861 tatctgaaac agctaaaacc ttgtcattac agcaaaaaat cacgttat
//
LOCUS       DAFBQH010000099         7623 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.104, whole genome shotgun sequence.
ACCESSION   DAFBQH010000099 DAFBQH010000000
VERSION     DAFBQH010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.104"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(14..706)
                     /locus_tag="LK694_002529"
     CDS             complement(14..706)
                     /locus_tag="LK694_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK5053352.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="LK694_002530"
     CDS             complement(1143..1712)
                     /locus_tag="LK694_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBK5053353.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="LK694_002531"
     CDS             complement(1786..2109)
                     /locus_tag="LK694_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053354.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="LK694_002532"
     CDS             complement(2263..3075)
                     /locus_tag="LK694_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053355.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="LK694_002533"
     CDS             3429..4277
                     /locus_tag="LK694_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBK5053356.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="LK694_002534"
     CDS             4422..5363
                     /locus_tag="LK694_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HBK5053357.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="LK694_002535"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="LK694_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1511 c         1371 g         2469 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aac
//
LOCUS       DAFBQH010000100         7597 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.105, whole genome shotgun sequence.
ACCESSION   DAFBQH010000100 DAFBQH010000000
VERSION     DAFBQH010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.105"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(940..1353)
                     /locus_tag="LK694_002536"
     CDS             complement(940..1353)
                     /locus_tag="LK694_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBK5053358.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1809..2294)
                     /locus_tag="LK694_002537"
     CDS             complement(1809..2294)
                     /locus_tag="LK694_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053359.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2433..2678)
                     /locus_tag="LK694_002538"
     CDS             complement(2433..2678)
                     /locus_tag="LK694_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBK5053360.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2871..3671
                     /locus_tag="LK694_002539"
     CDS             2871..3671
                     /locus_tag="LK694_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK5053361.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3690..5558
                     /locus_tag="LK694_002540"
     CDS             3690..5558
                     /locus_tag="LK694_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBK5053362.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRSYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5551..6042
                     /locus_tag="LK694_002541"
     CDS             5551..6042
                     /locus_tag="LK694_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBK5053363.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6030..6584
                     /gene="srlA"
                     /locus_tag="LK694_002542"
     CDS             6030..6584
                     /gene="srlA"
                     /locus_tag="LK694_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBK5053364.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6602..7597
                     /locus_tag="LK694_002543"
     CDS             6602..7597
                     /locus_tag="LK694_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBK5053365.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1214 c         1442 g         2233 t
ORIGIN      
        1 gttactatga ataacataat aagaaagtaa agtggattga tttgagatct ttttatactt
       61 caaaaaagat aacacccaac tttcaaaaat atgttttgga agttgggaca aacgagttaa
      121 tacattttaa tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga
      181 gagatcataa gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc
      241 catatttctt caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc
      301 cagcaccatt tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga
      361 agtaaaaaca aagctttact cataactaac aaaataccag ctccttttat ttttttctcc
      421 cccacattca aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta
      481 tgaataacag ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa
      541 gtttattttt ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag
      601 ccgatttcgt agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta
      661 tggataaaat agatcatcgg acaagcagat aagagtaagt atttgttagg ccttttgaat
      721 aagtaaaaga aagcactggt cattatgata aatggtatcc aaaacaaaag tttacttaag
      781 acaatcaaat gatcaactcc catatttttt accaagagag atcataacat ctttatcgta
      841 aaaacaaaat atttttttgc tcattttgaa aatttaaaca tactcgttct tatccaaata
      901 tataaaaatc gcccctcatt catcgagagg cgataagttc taatttaact caattttttt
      961 tgtttctttt ggctgataag ctgttttagg taataccaat ttcagcacac cgtttttaaa
     1021 ggttccagtg attttttctt catcaacatt aggaagatag aatgaacgtt tgtaagagac
     1081 cgaatgtctt tctttccgta catagttgcc atcttcttta tcttctgtat gggactcatg
     1141 attggcggag atcgtcaaag tattatctga atattcaact gtcgtgtctt ctttgtcaca
     1201 gccaggcata tcagcagtta gtttgtattc tttttcattt tcgactaaat ctacttttgg
     1261 ataacttgaa acacctaaaa aatcattgaa tgctggacta aacacgtcat tgaaatcagg
     1321 gaacatgtcg cgaatgctag gtacatttgc catttttgat catcctttca ataagtattt
     1381 tttatttatc cctatttacg taataactct attttgaaaa aaatgcaagt ataatggtta
     1441 gctttgtgaa tatttcatct aatacgatac aaaggctatc tttctaattt gaaaaaggga
     1501 tcattaaaat aataagaaga aaatatgtca aaaggtaaaa aagtaaattc ttctatccaa
     1561 atatagaaaa attgaatcta gaaaaccgcc cgacaataaa tttcattaac tcagttgacc
     1621 gaaaatgaag tagataccaa aaatttatca agagagcaag tagagaagtg agtatattca
     1681 taagagaaat agctgtaacg aattattagg ttaatgcaaa tgcttattta tactccgtat
     1741 aactttctca gaaaaaatga taccaataat gacttgctag tactgtaaat ccgtataata
     1801 tcatctaatc aaaatggtgt tccatttgca tctgtcatcg taatcgatcc ttcgggtgac
     1861 actcgataaa aaccaacgct tccagatcct ccttgggaac ggatttgttt gctggaagct
     1921 gtgaacaaat aatctcgatc aattttttga taaaagctaa taacaatatc gtcactttgt
     1981 aattcggaac ggtgggcaat aagaaataga agtgctttgt cagagttttc taatggaaga
     2041 gaaggtacag tgctttcttg catttcagat gttgaagaaa tgctaaacga ctgaatactt
     2101 gattggctat gttgcgaatc tacattacca gtagaagtat tagtaatgga agtagtagaa
     2161 gtacttttag ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc tttaacagaa
     2221 ttctttgttt tatatccaca ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa
     2281 atccctttat gcatgatagc tcctcctaaa atagattatc tttattcagt atatcactta
     2341 aacaaagaaa agcagatggt ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg
     2401 aagataataa cttttttaat aattatagct ttctaatatc tttcatcaac ttttctagta
     2461 atcattggga aaaatttcaa gcagtagatc gcacaaatac ctacgacgat ataaatgatc
     2521 ttcgcaaaaa tagttgttga accaccagca atcatagcta ctaaatcaag ttcgaacaag
     2581 cctaccaaca gccagttcaa ccctcccaca atcaataaag ttaatgcaat agcatctaag
     2641 ttttcattag aattctcctt tcttaaatga taaaacaatt atatatcaaa aagagaaaca
     2701 ttgcttatat aacgctcagg aattttagca atctatgcaa aaagatgaaa attttttttg
     2761 ctcttccatt agtacacatt ttttagctga agctgtaaat ttcatgtctt aatttttttt
     2821 agtgagcttt tatactggat ttgtaagaaa aaagaaggag aggatcacat atgtcggatt
     2881 ggttggatat tgcaggaaaa gtagtaattg taactggagg ttcctcagga attggaagaa
     2941 gtatcgtcga aaatttgttg aagcagaatg cacaagtagc gaattttgat gtaactgaat
     3001 gccgtataca gcatgaaaac ctactatctt taaaagtcga tgtgtcttct aaaacggaca
     3061 tagaagaagg gatatataaa gtaatgaaac atttcaagac gatcgatggt ctagtcaata
     3121 atgcaggaat aaatattcct tctcttctaa tagatagaaa tcatccaaaa agcaagtatg
     3181 aattatcaga acaagttttt gataagatga tcgctgtcaa tcaaaaaagt gtatatttga
     3241 tgagccaagc agttggaaga atcttggtgc aaaagggatc aggagtgatc gtgaatcttt
     3301 cttctgaatc aggattggaa ggatcagaag gacagagctg ttatgctgct acaaaagcag
     3361 cgatgaacag ttttactcgt tcttgggcaa aagagttagg aaagaggaat gttcgtgtgg
     3421 taggtgttgc accagggata ctggaagaaa ccggtttgag aacacaagaa tatgaagaag
     3481 ctctttctta tacgagaggt ataagcgtcg aacagctgag aaacggttac agtcgaacaa
     3541 gtacgatccc tctaggaagg agtggtaagc tgcaagaagt ggcggatcta gtctgctatt
     3601 atctttcaga aagatcaagt tatatcactg gagtcacgac taatatttca ggaggtaaaa
     3661 caagaggata aaggataggg gggacgcacg tgacgctagt aaacagatgg taccaattat
     3721 tgaaattact agtcactcac aagaaagttt caatccagga agtgcaaaaa gaaatcaaaa
     3781 ccagtaatca aaccataaaa aaagatattg gggagttgaa tgaagaaatt gccggtattg
     3841 caaaaatcat ccaaaaaaac aaacattttg aacttgaaat ttatgatttt gatttttttg
     3901 atgagatcat gcaaggaaaa ttgaaaacag ccagtgattt caattcggca tccaaaagaa
     3961 tcgcttattt actaaatagg ctgatcgaaa cagaggattt cttacgtatg gatgatttgt
     4021 ctgaagaagt cggtgtcagc agaggaacag tggtcaagga tctaaagaca ttaaaagaga
     4081 tgatcaatga ttttgatgta aagattacag gcacaccaaa taaaggacta agaataatag
     4141 gagatgagct agaattacga ctacttttat tttattttgt ttctccctac ttttcagaga
     4201 cgaaaacaga acagtttcta gaagaaaaac aagcaatcaa acattttcag aagaagacga
     4261 atgctagtaa acaagaaatc acccttttaa cgaaagttat cggtatttct ttgaatagaa
     4321 tctctagtaa ctatctgttg aagaagccca tcaatcgtta cagtgggtgt tttgaatata
     4381 tccaagaatt caatgaactg atcaatcagt tggaagaaat ctatgaacta actttgagcc
     4441 aatatgaaaa agattttatc agttttcctt tgaacatcat caacacaagt ataacgatga
     4501 taaggaaagc taactctgat ttgcgcttat atttcgaccc catgatgatg cgtattcgac
     4561 gattagatct cattgatctg gatgagtcct tcttatatca agagatgaaa cagcacctct
     4621 ctttattgat aaatcgggta atttttcgtt atcgattgac tgatttattt tatggtgaga
     4681 tagaaaaaaa gtatcctttt tcttaccaaa tcgctagcga atgcatacaa gcattagaag
     4741 ctatcttgca acataagatc tctaaagtag agatcagcta cctaacgttg tatttggagt
     4801 tagcatttcg atatcaaaaa gagcattttt cctctaaaaa agtagccatt gttcatacga
     4861 caggaaaagg aacagcatta atgatccaaa gacaaatcaa aaggatattg gggagggata
     4921 tacaaataac acatgtttca gaagaacaat atgaaaaagt agatctaaac aaatatttcg
     4981 ctgtttttac aacgattccg ttaaaaaatg taaatgaaga gacaccggtg atccatttga
     5041 ctaatctttt tgatgactct tggttaaggg cacaatggca aaaagcaaaa aaaaagcgat
     5101 tagccgggtt tcattctact gtttttttct atcaaatatg gaaagaaaaa aagacgtatc
     5161 aagaatattt aaacaaatta gtcgctcctt tgatcagaag ctattatgtg gatgaagaat
     5221 ttgaaaaaag gatcttgaaa agagaatatc agcaaagcac atttgttaac aaagaatttg
     5281 cttttcctca cacaaccaac cagctatctc aacacattgt tttatcagta gggctaataa
     5341 ctgatcaagg tttagcagaa acaggaggaa atggagttat ttttttattt gccatacctg
     5401 aagaaatgac ggctgaagtt gagaatgaat tatttgagct atacgatatc ctatttgaac
     5461 tcataaaaat gggaatagat gagtttattg aattagcacc gacacaagaa tcatttttaa
     5521 aactaatcaa aagtaaagga gagagacttc atgaatgata tgtatctatt catagttttc
     5581 gcagcttcag cttatgtgct gcaagcagtt atcagttgga agcaactaaa gcatttcaat
     5641 caaacatata gcgaactgcg aagaaaagga aaagtagcga ttggtaaacg acctgggaaa
     5701 atccgttcag gcacgattgt tatgctagct atagacgatc atgatcaagt tttagaagtc
     5761 agaaaaatgc aaggggttac atctttggca aaatttcatt cattacctgt ctatgcgggt
     5821 gaagatatcc attacatcga tcgttaccat ccctcagttc gagcggaaga tcgtttgaca
     5881 caagcagctt tagaaaatgc cagagaagta tacttacgaa tagaggcagg aaatgaaaaa
     5941 agtgaacaac cttcattgcc ttttattgct atgctcaata attttcggat atggaaaagt
     6001 acgatcaaag aaaaaaggag tgacaaacga tggatatcat aacaaaattt gctgagggat
     6061 ttatgaggct gttccaaaca ggtgcagaaa cattcataag ctggatgaca ggaatcgtac
     6121 cagttgtctt gatgctgatg gttgcaatga atacgctgat tgctttttta ggtgaagacc
     6181 gagtaacgaa agtagccaaa ttatcagcga aaaatccgct tactcgttac cttgtgttac
     6241 cgttcttatc tgcttttatg ttgggaaatc ctatgtcatt tacaatggct cgtttccttc
     6301 cagaatacta caaaccaagc tattatgctg ctcaagctca attttgtcat acgagcaatg
     6361 gtgtattccc ccatattaat cctggagaat tgtttgtatg gatggggatt gctcaaggga
     6421 tcgaaacctt agggctaaat tcgatggagc tggctatccg atacttgctt gtgggactgc
     6481 tgatgaactt tattggtggt tggataactg attttactac tggatttgta tgcagacagc
     6541 aaggaatcgt tcttagcaaa aaagtagagc tttcagttga ttagagaaag gagagtttta
     6601 aatgtatggt gatattaaga tcgtaagagg aaacggcggg tatggaggtc ctttgattat
     6661 taggccgaca aaagataaac acaagttcat ctatgtcaca ggtggaggtg agaggccaat
     6721 gatcgtagat aaaatagaag aactgacagg tatggagtct atcaatgggt tcaaaacatc
     6781 aatacctgat agtgaaatcg cgcttgcaat cgttgactgt ggagggactc ttagatgtgg
     6841 aatttatcct aaaaaaggga taccaaccat caatatcgtt cccacaggaa aaagtggccc
     6901 attagcacaa tatatcactg aagatattta tgtttcagca gtagggttag aacaaataca
     6961 gccactaatg gaaactgatg ataacaaaga aacagtaaat actgcatctc cagataaaaa
     7021 gtacaaatat gatacttcta aaaagataac cgagcaaaaa gcagagcagc aaaactggat
     7081 agcaaaaatt ggaatgggcg cgggaaaagt agttgctaca ttcaatcaat cagcaagaga
     7141 agcagtagat acgatgatcc ggacagtgat cccttttatg gctttcgttt cccttttgat
     7201 tggtgtgata caaggttctg ggatcggtaa tgtatttgct aacattatgg cacctttagc
     7261 aggaaatgca gttggattaa caatcattgg ctttatttgt tcactgccat ttttatctcc
     7321 tttgcttggt ccaggggcag ttattagcca agtgattgga acattgatcg gtgtagagat
     7381 tgggaaagga aatattcctc cccaattagc attaccggca ctatttgcta tcaatactca
     7441 aaatgcatgt gattttattc cagtcggtct aggtttggaa gaagcaaaag ctgagacagt
     7501 agaagtaggt gtaccatcag ttctttattc aagatttctt aacggcatcc cacgagttat
     7561 cattgcttgg gtagcaagtt ttggattata tagctga
//
LOCUS       DAFBQH010000101         7188 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.106, whole genome shotgun sequence.
ACCESSION   DAFBQH010000101 DAFBQH010000000
VERSION     DAFBQH010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.106"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="LK694_002544"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="LK694_002544"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBK5053366.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="LK694_002545"
     CDS             complement(1172..1867)
                     /locus_tag="LK694_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBK5053367.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="LK694_002546"
     CDS             complement(1880..2596)
                     /locus_tag="LK694_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBK5053368.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSILYK"
     gene            2926..3534
                     /locus_tag="LK694_002547"
     CDS             2926..3534
                     /locus_tag="LK694_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBK5053369.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="LK694_002548"
     CDS             3859..4182
                     /locus_tag="LK694_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBK5053370.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="LK694_002549"
     CDS             4364..5734
                     /locus_tag="LK694_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBK5053371.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            6081..>7188
                     /locus_tag="LK694_002550"
     CDS             6081..>7188
                     /locus_tag="LK694_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBK5053372.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKK"
BASE COUNT         2110 a         1445 c         1344 g         2289 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcgttatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttatata aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaacat actaagatta gaggggaaca tcattgacga atgatgttct cctctatttt
     5881 ctatttttag attatagtga agagtgaaag aaagttcgaa caaagccttg aggactacga
     5941 gtccctatca aaaaccgaac ttctttactg tcattttaga atcaggttta cgtatgttgg
     6001 ggccatgagc ccgtgtatag gaaattggaa tcgaaatgaa gcagtaaagt tctttcaaaa
     6061 cttaaaaagg aggaaaacaa atgccttaca ttcttgcatt agatgtttca atgggacaca
     6121 gctacagtgt cctatacaaa gacgacacct gcttatttga agacgagatt gaacacactc
     6181 aaagaggatt tcatgcatta ctcgaagaga tccagcactt gcctgagcgt ccattgattg
     6241 tttttgaagc aacggggatt tattccagac cagtcgaaaa gttctttcaa gatcatcact
     6301 tttcttactg tttgttaaac ccgttagagg cgaaaaaaca gatggaagaa agcaccttac
     6361 gcagttggaa aacagataaa tcggatgccc accggctcgc acagactcat cttaaaaatc
     6421 aacgtcagcc aaaagaagta caaagcaatt tttacttaga gatgcgcgat ttagcccgtt
     6481 tttatcaaga aattgagaag aaaatcactc gtctgcgcat ggatttacac aactgccttc
     6541 aattgacttt tccagagctt gaacagttct tttcaaacag gctgacccct tacgcattga
     6601 cattgatccg tctttttccc catccagatt ttgtattggc atctactcgg acaaaaataa
     6661 aaaataaact gatcaacgaa acacggaaaa aaatctccgc gaatcgtgcc gagcaaaagg
     6721 cagatcaaat catccactat gcacagtgtg cctatccagc tgttgaaaaa gacagtatcc
     6781 actgtcaaaa aacgatctat tacgccgaac ttctccaaga tttactcgaa caaaaaaaag
     6841 cactggcgac acaaatgatc aaaaaagccg aaggctctcc ttgctttttg ttgtaccaaa
     6901 cgttccctgg gattggcgca ctcacagccg ctttattgct cggtgaatta ggagatatca
     6961 cacgatttaa aacccataaa caattgaatg cctttgtcgg aatcgacatc cgacggtatc
     7021 attcaggtaa gtatactggc caagaccgca tcaataagcg tggtaatccc aaagcacgga
     7081 aaattatttt cttcaccatt cgaaatatga ttcgccagca acgcgcagct cctaatcata
     7141 ttgtcgatta ttactacaaa ttaaaaaagc aacctatccc taagaaag
//
LOCUS       DAFBQH010000102         6773 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.107, whole genome shotgun sequence.
ACCESSION   DAFBQH010000102 DAFBQH010000000
VERSION     DAFBQH010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.107"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(559..1599)
                     /locus_tag="LK694_002551"
     CDS             complement(559..1599)
                     /locus_tag="LK694_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBK5053373.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLI
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1948..2274)
                     /locus_tag="LK694_002552"
     CDS             complement(1948..2274)
                     /locus_tag="LK694_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053374.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2261..3064)
                     /locus_tag="LK694_002553"
     CDS             complement(2261..3064)
                     /locus_tag="LK694_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK5053375.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3417..3707)
                     /locus_tag="LK694_002554"
     CDS             complement(3417..3707)
                     /locus_tag="LK694_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBK5053376.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3843..4022)
                     /locus_tag="LK694_002555"
     CDS             complement(3843..4022)
                     /locus_tag="LK694_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053377.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4019..4369)
                     /locus_tag="LK694_002556"
     CDS             complement(4019..4369)
                     /locus_tag="LK694_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053378.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4362..5687)
                     /locus_tag="LK694_002557"
     CDS             complement(4362..5687)
                     /locus_tag="LK694_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBK5053379.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6137..6751
                     /locus_tag="LK694_002558"
     CDS             6137..6751
                     /locus_tag="LK694_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5053380.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIITSVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
BASE COUNT         2077 a         1218 c         1019 g         2459 t
ORIGIN      
        1 cgttgcgggt aataccgtaa cactgaaggg cacgcattaa ggcgtcatct taatgctttc
       61 ctgtaggaca ctgacggaga ttaccagtct cccgagcctt acaggggcct ttcttttttt
      121 tgtattttca attatcacaa tttatcgtcc aaaagtgcag aacgaaaatc aattcatcac
      181 aagcatatca gacaattaaa tttaaaacaa ctaaaaatat acaaaaaagt gtaattgaat
      241 attagcagca ctgcctgaaa agaagtaata tcaggctttt cagcttgggg aaataagtga
      301 attacctctc ttcatgaatt attataactc aaaaaagcac atatgcaaac actagttccc
      361 tatttttttg aatgaaaaat aactaaaata tctaacgatt atgactttaa aacctacgtt
      421 tgtgattgtt ttcataaact tttaatctaa aaactccgga ataatagcta tttatttgta
      481 acatttgtaa taaaattaaa atcataaaaa agttctaatt taaaattgat catttttata
      541 gtgatcgctt tttatcaact actctagcca attttccgta ctaactgttg gtaattcgtt
      601 aaatgtttct ctttgttcgt ttgcaagctt tgccgcaata gctttttcga caaaccaatt
      661 ttttacataa acgaaaatta gattctgcaa attgtttact ttttcagttt tttgtttttg
      721 ataagctttc agcaaagtca tatataatcc tttgtcagca tcaattccat aagattccag
      781 ttcttcataa acaacaactg tttgctctaa ttcttcagct tttttctttg cattatgaat
      841 agtttggacg gttttttgag cgtcttcgta agtactagaa aatgttttta tcaagtttaa
      901 tacattttct ggtacaaatg tatttatact gtcctctttc atgatgtcga cgaagttatc
      961 tagtaaaact tgattttgta actgatcagt ttcagtgtct attatgtgtc tattagtgtc
     1021 tatagaatta gaatagaata gattgtctgc gattttcgca attacggttg agtccttgat
     1081 atgactggat tttgccgtat ttgcgatttt cgcaattacg gtggtatcat tgatttctcg
     1141 cggttttgcc gtatttgcga ttttcgcaat tacggtggta tcagtattgt cttcctcttc
     1201 catgatttta aagacgtctt gattcgttac ttctggcttg cctaagtaca ataaattagg
     1261 catggattca atcttaccgt cacgtttatt tactcttcct ctcttctgtt tcagaagacc
     1321 tgctttttct aattcctttt tgattttaga tactttacct tcgcggcatt gtaacaattt
     1381 cattaattct tcaactgtga atatgaaaaa tatattatca tcagaatcta cccagttatt
     1441 tttgactgaa taatcaaatc gatctttaat tagcatataa gcaatttttg catcatttga
     1501 tagatccatg tatttttcgt tagtaaaaaa taccttaggc atttgataat atttttcacg
     1561 ataaatttct tttgatttgt aataattaaa atcattcata gccaatcctc tttctgtagg
     1621 atagcgtttt gttcaaaact atgttacaat gaattaaaca aatatatcta tccactatat
     1681 atttttctac tgctgataag tcgccaaact ttgttaaagc agtagtcttt ttttatcttt
     1741 ttttagttta ccttaaaaaa taaattaaag caaaccgctc tatttcaagg tttaagctca
     1801 tagtaactat atcacgatat tacaataata ttatatcacg atatcgtaat attacaatat
     1861 gaagataagc gacttatcag ctttgatact actgattttt ataaaaaaaa tatcacgata
     1921 ttatgatatc gtgatattga atgtaaatta aattttattt tgtttctttg ctagtttata
     1981 tccttcaatc aagtattctt caggtatgga ctttaatagc gcatcttcta taacgtctgg
     2041 tttacttttg tctgattcag caatagtcgt ccaatatttg agtgctgaca ttagaattgg
     2101 atttatacga atgctatctg ctgttcttac actacttcct tgatctttta tgactttact
     2161 tatatttttc ttggttaatt cgatttcttc tacttctttt gatttaaaat tctttttatt
     2221 tttatttcct agtaatctac ttactgcagc ttgactgcga ttatcttctg ccatcaaaat
     2281 caccaacttc ttttaaatat tcttctgtaa atgctttata gtcatcttca gctgttgagg
     2341 gtttattaaa attgattaga tctgatccag cagcttgcgc ttcgtcaacg gttattgtcc
     2401 tacgaatata agttttgaaa atttttgcac caatgactct agaaatttcg gttgctgcag
     2461 acgtcatttc tttagaaatg ttataacgct tattatactt agtaaataga actcctcgta
     2521 cttcaagctt gtcgttcgta tattctttta ccgtttcaat agtttcactt aactttatta
     2581 gtccttttaa tgagaaatac gcagcttcaa ctggaatgat aatttcatca gaaacagtaa
     2641 atgcgttgac tgtaagcatt cctaaacttg gtggacaatc aattataata taatcatagt
     2701 tgtccttgat tggttgaagt actcttttaa gcctgtgttc acttcctact tgagtaaatt
     2761 ccctctctgc agaagaaagg gctatgtcgg ctggcaaaac atcaattcct ttatagtcca
     2821 gaatagcatc tgtaatgtca gcctcccctt ttaatacttc ataaattgta ggttggtttg
     2881 ttccatctat atcgaaacct acattatctg tagcatttcc ctgaggatca atgtccacta
     2941 aaagtacatt tttaccgtaa acaaatttca aacatgctgc taaattaacg ctagtcgtag
     3001 ttttagaaac tccacctttt tgattggcta ctgacaatat tcttgccatt aaaatatccc
     3061 ccattcatct tatattataa tataattata tcataatatt gtgatattgc aatatcgtat
     3121 atgcattata ttgtaatatt gtgtgattat tttgttttaa agatatatat cggttcggat
     3181 ataaagatta ttcatatgtt attcgaaatt aaaataatca ctttttatta ctttaaaatt
     3241 ttttattaat gataagaaat atgttttgcg agcagcaact agaataattt aaacattact
     3301 aaactgtcta tattgttact aataattccc ttcttatagt gcattttctt agaaaaaaac
     3361 aatattacga tatcatgata ttaagatatc gtaatattat acaattgtga ttgatattat
     3421 atatgttttt ttattttttt atcatgttta atcttgaagg aatcatattc tttttctaaa
     3481 gacaggagct tttcataaac atcagacatg tttaaactag aggccaggct taaatcataa
     3541 acgaatgaag caggtaatga ttttattcta cgttgtcggg ttatccagct tgcaaccgtt
     3601 ccttgtgggt aggcatataa ctcacaaaat tgttctacag tgattcctaa attcttgata
     3661 atgtaaacat tgatcgggtg cgagtaaaca aatgtattga tagacatggt gcgtaagcct
     3721 actttctttt attattgcgt ttgcaataat agtttaacac acgcattatt ctagtatcta
     3781 ctcctatttg taaaaacaaa aaaggattca tagagataac tctatagaat ccgtaaaata
     3841 ccttatatat ataacgggaa gatataaaac ctaaaattat gataacctgc tgcagcttct
     3901 acatagaaag agaagcgctc aacaccaacc tcatttatca aacgttggcc ataaccccaa
     3961 atctcttctg atagctgatc aaaggttata tactttagct catattgctg gataatgttc
     4021 attctaatcg actccattta ttatcctggt aaaattcgat atgacgtatt tcgtcgtagt
     4081 gaattttgtc ttttccaacg tagatcccca gctcatcact acctaagatc ttgccaatga
     4141 tatcttctgg atagtatcct tcagcgtttc gttcttcttt ttgaatagat actgatctat
     4201 tttttatgac tgcagcgttc aatacttccg caatctcttc agtcgtcatt tgttcttttg
     4261 ctaaattttg actggttcgt tgttcattgt cggcatcaat ttcttcagta tgatcagata
     4321 aataaaaccc tgcccacttt aaacgtccac gatcattgta atcaatcatt tttaattccc
     4381 ccatttccgc ccgcatgacc accaacaagt gtgcttctag taatgcttct agcgccttct
     4441 aacattgaag aagcatgtac aatcgacgta aaaccatatt tttgccgaat cttgtcaata
     4501 attttatcta aattttcttc gtcaatttgc ttttgtggat cactaaataa atccagctga
     4561 agcgaatcgg tgtacacaag cttgctatag gttattccaa cgtgccttac ttcttgtcct
     4621 tcataatact tatcaaacaa gaaaagacaa taattctgca attcctttgt attatctgtg
     4681 ataggtattt tcatctggtg tgaaaatcct ggtctcgttt ctaagatcga cgtaccaatg
     4741 tttaaatgaa cacatcctgt tttacaatta tgctgcctga tacgaatggc tacttgttcc
     4801 gccatttctt ttaccaccag ctcgatttca tttcttctag tataatctcg tggtaacact
     4861 tgtgaattac cataagattt ttcctttgtc ggttctggcg ggattgctat atctgttcga
     4921 tcgattccgt tagcatggaa gtacagctgt aagccaatca cacctagtct attcttaatc
     4981 gtataaggat tccagttagc aagatctcta atgctcataa tccccatctg attgagtctt
     5041 tttttcattc gagagccaat tccccaaaag tccgtcattt caggaatatt ccatactttt
     5101 tcaggaacgt tttcgtacgt ccattccgca acaaatcctt cattatgctt tgcttcgttg
     5161 tctaaggcta gtttagccaa aaggggatta tctccgacac ctactgtagc aatcaatcct
     5221 agctcttctt taatattctc ctggatcatt tgagccagct tctttctccg ttgacttcgt
     5281 gttccttcag tcgtaaaaag attcagtgat cgggtcactt ttaagattga ttcatcgatc
     5341 gagtaaatca gtagatcttc atcagccaca tatcttctga aaatattatt tacctgcata
     5401 tttcgcttga tatataggtt catacgtggt ggaacaacgt gtagtgtttt aggaaatggt
     5461 tgtggtaagt cacgtggtct actcacattt gtaataccat accgcttttt tgcttcagga
     5521 gaagaagcta atatcaatcc tgaaccagta ttatcagctc gactcataac aacaagttct
     5581 gttgttaatg gatctaaatt tctttctata cattcgacac ttgcgtaaaa gcttttcata
     5641 tcgattaaaa agtaatcatt tactggctct tttgaataat ccatcataaa taacactcca
     5701 ttctttcaca ttatacaaac atatgttcga tattgtaaag aagaaatagc taaatacata
     5761 aaaaaagaag cataggtatg ctatcaccct gcttcttctt ttgatttgta tgatcaattt
     5821 attctatcac tttactaatt aatttgaaaa tctaataaaa actctcactc taaaagagtg
     5881 agagactgca aattagagta cggacttttt tggagttccg ttctttaata ttaacaaatg
     5941 aataaaatcc ttacaacatt tgagaataag ttatcagtag acacttacaa aaaattctca
     6001 tactatttcc tccatcatta gtttttgtct atataaggta taccctaaat agacactggt
     6061 ataacattga aaaatatggc ttcctcgtgt atgattaggg tgtattttaa atagttgcag
     6121 aaagtaggta aaagttttga gagttgcata catacgtgtc tcatcgatcg atcaaaatga
     6181 acaacgccag attgaagaaa tgaaaaaatt cggtgcagag agaattttca tcgagaaaca
     6241 atctggcgca acgattactc atcgacctgt tttccaagaa gcattagatt acgtccgaga
     6301 tcaagatatt tttattgtgg aagctattga tcgacttgga agaaattatg acgagatcat
     6361 cacttcagtc aattacttga agaagaaaaa tgtgaagctg atcattacta gtttaccgat
     6421 catggctgaa gcaattggca atcctctcct ggataaattc attaaggatc tgatcatcca
     6481 aattttggcc atgatcgctg aacaagaacg gactgaatcg aaacgtcgcc aggcacaagg
     6541 aattaagatc gcaaaagcaa acggcgtcta caaagggcgg cccaaattat acagtgcaga
     6601 cgcaaaagat ccccaacgtc gccttgttta caaaagcatc gttgaagatc ttaaaaatgg
     6661 tgttgcgatt gctaagattg ctaaagacta caatgttacg agacaaaccg tgtatagaat
     6721 aaaaaaagac agcatggtca atgacaaata gttgatcatg ctgcttttat tat
//
LOCUS       DAFBQH010000103         6644 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.108, whole genome shotgun sequence.
ACCESSION   DAFBQH010000103 DAFBQH010000000
VERSION     DAFBQH010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.108"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(1..859)
                     /locus_tag="LK694_002559"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="LK694_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="LK694_002560"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="LK694_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBK5053381.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="LK694_002561"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="LK694_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="LK694_002562"
     CDS             complement(2143..2373)
                     /locus_tag="LK694_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HBK5053382.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="LK694_002563"
     CDS             complement(2603..3421)
                     /locus_tag="LK694_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK5053383.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="LK694_002564"
     CDS             3582..4271
                     /locus_tag="LK694_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBK5053384.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="LK694_002565"
     CDS             4285..5787
                     /locus_tag="LK694_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK5053385.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="LK694_002566"
     CDS             5800..6276
                     /locus_tag="LK694_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK5053386.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="LK694_002567"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="LK694_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAFBQH010000104         6466 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.109, whole genome shotgun sequence.
ACCESSION   DAFBQH010000104 DAFBQH010000000
VERSION     DAFBQH010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.109"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(109..564)
                     /locus_tag="LK694_002568"
     CDS             complement(109..564)
                     /locus_tag="LK694_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053387.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="LK694_002569"
     CDS             complement(718..1926)
                     /locus_tag="LK694_002569"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBK5053388.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="LK694_002570"
     CDS             complement(2038..2370)
                     /locus_tag="LK694_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBK5053389.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="LK694_002571"
     CDS             2518..3393
                     /locus_tag="LK694_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK5053390.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="LK694_002572"
     CDS             complement(3475..4056)
                     /locus_tag="LK694_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBK5053391.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="LK694_002573"
     CDS             complement(4062..5321)
                     /locus_tag="LK694_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBK5053392.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="LK694_002574"
     CDS             complement(5323..6369)
                     /locus_tag="LK694_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053393.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1803 a         1337 c         1102 g         2224 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccg ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaa
//
LOCUS       DAFBQH010000105         6378 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.111, whole genome shotgun sequence.
ACCESSION   DAFBQH010000105 DAFBQH010000000
VERSION     DAFBQH010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.111"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(312..842)
                     /locus_tag="LK694_002575"
     CDS             complement(312..842)
                     /locus_tag="LK694_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBK5053394.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(1024..1680)
                     /locus_tag="LK694_002576"
     CDS             complement(1024..1680)
                     /locus_tag="LK694_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBK5053395.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(1807..2280)
                     /gene="dfrF"
                     /locus_tag="LK694_002577"
     CDS             complement(1807..2280)
                     /gene="dfrF"
                     /locus_tag="LK694_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBK5053396.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(2699..2917)
                     /locus_tag="LK694_002578"
                     /pseudo
     CDS             complement(2699..2917)
                     /locus_tag="LK694_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2948..4162)
                     /locus_tag="LK694_002579"
     CDS             complement(2948..4162)
                     /locus_tag="LK694_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBK5053397.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(4503..4745)
                     /locus_tag="LK694_002580"
     CDS             complement(4503..4745)
                     /locus_tag="LK694_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK5053398.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(4777..5334)
                     /locus_tag="LK694_002581"
     CDS             complement(4777..5334)
                     /locus_tag="LK694_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBK5053399.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(5347..5535)
                     /locus_tag="LK694_002582"
     CDS             complement(5347..5535)
                     /locus_tag="LK694_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBK5053400.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(5548..6114)
                     /gene="amaP"
                     /locus_tag="LK694_002583"
     CDS             complement(5548..6114)
                     /gene="amaP"
                     /locus_tag="LK694_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBK5053401.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         1894 a         1339 c          953 g         2192 t
ORIGIN      
        1 ttttttgaaa tcaactgtct ttctcatatt tactattcaa ttttcatagt gatataggta
       61 caatttatat cactactgta aaaatggggt tttgccttat aaatacagcg ttttccgctt
      121 gttagtgtat agtgtttctt gttttttgtg ccccccttgt tagataacgg gggctttgaa
      181 cggctcgcaa gctcgccgaa ccgccgaagc gaaccgcccc cttgcaagca tggcggaaaa
      241 atcttaagga ttttcccgcc aacgtggtcg gatcgctccg tcggtttaac agcttttctg
      301 tttctctcta gttatctacg catataaatc aaatcggtta ttccgttata agtctgtgtt
      361 ttacaaagcc ttaactttat ttccctgtct atgttgccca aaagacgaac cccatttcct
      421 aacagagtag gaataacaga aatgtaatat ttatcaatca tatttttgct taccaactgc
      481 tgaacaagat ttgctccgcc acaaatccaa atatcctttc ctgcttcttg ttttagccgt
      541 tccaataata aaacaggatt ttcacttgta aaatgaattt gttgggaaga attgcactct
      601 ttatgtgtta atacataagt tgtaaattta ttatataccc attcttgcgg agaaagttca
      661 gtaacaacct gatgagaagt attccacccc attaagacag tatctatatc tttggtaaat
      721 tctgtataag tgtcgatgtt ttcattgtca tttccatgac catttagcca agctactcca
      781 ccattaccgt ccgcaatata tccgtcaagg ctcatggcaa taaataaaac tacttttctc
      841 attttttctc ttttataaat acaaacagga atttatttct cttttttact cataagggaa
      901 caactgattg ctacaaataa acccattaaa atccatggaa atgctgctct tataaaatca
      961 ctaaccatac tttattcctc cttaaattcc aatttcctta gttctacaaa ctgaaatttt
     1021 tgcctatgaa tgtatcttgt tctttatacg caaacaggct tctgtgtgca taatccaatg
     1081 tcttgaaaac ggcatttgaa ttagtccaca aatatcttta ttacaccaat aatcaatcag
     1141 ccaaattgct ttttcattgt cgtttactac attcaacgat tctaagtatc cttttctttc
     1201 tttcggtatt tttcttttca attcatcata agataaccgt tcaagcattt tctcagtgct
     1261 ttcccatact tcgaaaatat ataaatataa ttcttccaga ttaagctgtt ttgaaaagtc
     1321 tgcaatctgc tgtttcatga gttcatttcc tgttgtaatg ataggtgaat tgatacgctc
     1381 ttgataattg cctgcaaaaa atacttgttc atcttcattt atcactgtat gcacaactat
     1441 atcttcaatg cggaaaatat gccaaataga ataagcaatc gtcttactat gataaccatc
     1501 agcgtttatg aaggggattg agttaaaatc ttctctgcat aattcttctt tgaaagatac
     1561 taaggtttgt attaactgac ttcgtaaagt aagtaaagta tcaatacccc tcttataagt
     1621 atctttcttt tttatttgtg cctgcattgt tttattaagt tcagaccatt ccttattcat
     1681 attcaatcct ctctcaaatt ctgattttat cgccccatac aaactggaat ttgtcttaca
     1741 acaatttata tatgcataaa aagcaatata tttatgaaaa caccctaaat ccaaaattta
     1801 ctcaattcat tttttcctta cataaaatgt tctcgtatat ttcacttcct caccaagtgt
     1861 ttcccctatc aaaacttcaa aatcattgat atcaaattct ggaaagaatg tatctccatc
     1921 ttcaatattt aaatctactt ctgtgatata cattttatct actatttgca aagcttcctt
     1981 aaatagtcca tatccaccag atatgtatac atctcgtcct ttagccaata ataatgcatc
     2041 ttctaatgat ttaactgaaa ctaaattatc tccttgatac tctgttgtgg tggaaacaac
     2101 aatattcatt ctattaggca acggatgacc gatttcttca taagactttc gccccataat
     2161 aaccacatta cccgttgtta actctctaaa ttgcttttgt tctcccttta ttttccatgg
     2221 tatattacca ttcttgccta taacattatt ctttgacctc gcaacaatca aacctatcat
     2281 ctatttcatc ctccataata tgatgtcgta cctgtgattc catcaagtgc accacatctt
     2341 aacaaactca aaattatctc gaactaacca gctcgacaaa ttcctttttt gtcttcatct
     2401 gcattatatc atattgttat aagcattgcc atttcttttc tgcttttgaa aagttactta
     2461 tcagcctatg ttttgtaaag ggtgatattc agcatttctc acgcaacaag cccttgacaa
     2521 accatagact aataagtttt aaggtaatca agcagaaatg aaatagtctg ctgtttcacg
     2581 gcaacaaaaa agagatacca acacacttat gtagtatctc ttagtctagt agcaatattc
     2641 ttttgtttgc aagcaggtaa acatgggctg tttcttaata tctttttgtt tgggaaacac
     2701 ctttttccca tcctttcacc gttgccatct ctgctgcgca caatgccaca gggataaacc
     2761 atagcaaacc gcctaataac aagaaaaata ctaagctgaa gcctgaggtc gcgaatgtgg
     2821 gatattcata taatgaaacg accattgaga tggtcatagc caaaaagcca aagaacgtta
     2881 atttttttgc aggaattttc gttttgcttt gttgcatgaa tcttcctctt ttctatttga
     2941 atttgtttca attgagtttg ttgccaaaga agaaaaagtc caatccttct tcttgttctt
     3001 cttttgtagt agtgatcgtt gtaaagtgca tcgccgattt taaaatcact gttgtcagca
     3061 cataggcata cacggcagta atgatgacac caatcacttg aatccctaag aagtaccagc
     3121 tgcttggatt cgttagtaaa tggctgttag atgcaaagat ccctatcaga atcgtacctg
     3181 tgaatccgcc catgccatga acaccccaca catcaagtgc atcatcccat tgtttctttt
     3241 tacgaaaatc aacagcagca tgacagacga tacccgcaat gatcccaatc agcatggcac
     3301 tttttgcttc tacataaccg gcacaaggcg taatggctgc caatccagca acggatcctg
     3361 ttagtacgtc gacaaaatca aagccatttc cttttacttt tgcaatgatc agccaagtaa
     3421 tcatcgcaaa tgcaagagcg atgaatgttg aaccaaatgc attgactgct tgttcatctg
     3481 ctcgaagtgc accaccggaa ttaaaaccga accagccaaa ccaaagcagt cctgtaccga
     3541 tagcagctgc cataagactg ctatgctgac ttctttctcc taaaggaatc tttctttgac
     3601 caagtgtgag gatacatgct aaagagccga atccagctgt cgtgtggatg actgctccac
     3661 cagcgaaatc tctaaatccc atctgatcca aaaatccgcc accccatatc cagtggcaaa
     3721 cagggaaata aatcagcaga ttccaaaaaa taacaagtat aatatagcct tttaaattca
     3781 atctccctgc aaaagctcct gtcatcaaag gaacggtaat cacgataaac atcagttggt
     3841 aaagaaagaa cattaaaaat ggaatcgttg caccatgaaa catgttgggg aagcttgtaa
     3901 catgtctcat caaaaagaaa tctgccgggt ttccaataac accgccgata tcccgtccaa
     3961 aggccaatcc gaatccacca aatacccaca taatcgttgt tatcccaacg gagagaaaag
     4021 attgagacat gatcgttagt atattattct tccgtgctaa tccgccgtaa aaaaaagcta
     4081 gaccaggagt catcaggcaa accattgctg tacataaaat catgaatgct gtatctcctg
     4141 tattgatagg taatgtattc atttttttcg cctcctttct acgattacga gtataaaaga
     4201 aggtgtagtt gtttttttct tctgtttttt tgaattaata cttgcattta taaaagacag
     4261 acaattaata aaagcttgat gtgttgtgct ctttttcgtt tttcacacaa ataaagacaa
     4321 aaaataaacg gtcttctttt tatagaaaac cgtttatttt cgtctttttt tatttttaaa
     4381 gatatttttt cataaggaat aaagaatact tcatataaaa tatataaaaa agaaccgcag
     4441 cgagtgtcgc tacggttctc aaatttgttc ttcatttcaa cataagtttt tcttcaattt
     4501 gcttataatt tacgtgcaaa gaatgaaacg actaatacta aaatgattgc accgatcaat
     4561 gatggaatga tagccattcc tgcaattgtt ggtccccaag ttcctagtaa agcttgtcca
     4621 attgctgaac ctactaaacc agcaatgatg tttgcgatga tccccattga tgatccttta
     4681 ttcgtgattg ctcctgcaat tgctccaatg ataccgccga cgattaatgc ccaaataaat
     4741 cccattttaa attcctccta actgacttct tacttcttat tgtacacgtg gttcgttatt
     4801 ttctttgact ttttgtgcac ctttgcctac agcagatttt gctttatctg tttggcgtga
     4861 agcaaattgt cctgtggatt ttgctgcttc tgttactttg tcttgtactg tttcttgatc
     4921 tttttggtat tcttcttgtg ttttgatgtc tgcaacattc acattcactt cgataacatc
     4981 taaatgtgtc atattgttta cttcagtact gatcaattct ttgatttggt tgtaaatatc
     5041 ttcgatgtct ttaccgtatt ctacgatcac atctagatcc acagctactt gtttcttacc
     5101 tacttctgta ttgatacctg ctgttacgtt atctgtgttg actaattttt cagccatatt
     5161 tgagaaaaat ccgccatcta atgtcaatag accatctaca tctttcaatg cataaccgat
     5221 gattttttgg atgactttgt cttcataagt caattcacca ttcatcatgc ggttttctcc
     5281 tttttcattt gccaaaactg atccatttgt tgtattcatg tttttgtttt ccatctttgt
     5341 ttcctcctat gatttaaaaa attgatcaac tattcctgac tgtttcaaat aaaaaccgat
     5401 tcctgcgcca attgctgtca tgattatgat caacaatgtc ttgaaaaatc caatcgttaa
     5461 ccataaaaca gctagcagta gtccaagacc cccaaagata atagggagct tgtatgctga
     5521 aaatagttct ttcattattt ggccctccta ttccactcga gagtgcttgt agtctcttgt
     5581 atcttcttgt tcttcataac cgctgtatgt gactgccact ttgaccgttt cttttgttcc
     5641 gagtacttta gttacttctt cttcgatatc ttgcatcaat acacctgttt tccctactaa
     5701 tgaagaagtt cgtttcaagt ctccgttgac atggatatgg attcgatttt tcgttgaacg
     5761 tatccgtact ttaggtgaat gaatgaactc ttcttcgtgt aaatgggaac gtaccaaccc
     5821 ttcgatcgct tttttgtcga ttgttagttt gccatcttcc cgtttcataa cgaaagttcc
     5881 tcttgacttt ggatagaaaa gaacgactag caagatgata atagcaagaa cggcaaatac
     5941 tacagctgcc cagaaaatat attgttgcat gtagtaattc gttaaagtaa agaagcgaaa
     6001 tcgttccaat tgttcaggca taatatacgg cgcattgatc aacgcaatcg ctagaaatac
     6061 tgaaagtagc agtaaactca tgataacacc gattattttt aaccccttat tcattttcca
     6121 cctcctataa acttattttt accctgtaaa taactctacc gacaatcaca gtatagatag
     6181 cgaacagtta gcatagcatc agtaaataaa gcgctaccaa acattgctgg atcataacgg
     6241 cgttttattg ccagtctgat actgaaataa caattgctta cttattctct atttactttt
     6301 gattgctggc tatacttaaa ggatattatt caagattaaa aattgcaaag attatgctca
     6361 gttattatta caagattc
//
LOCUS       DAFBQH010000106         6046 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.112, whole genome shotgun sequence.
ACCESSION   DAFBQH010000106 DAFBQH010000000
VERSION     DAFBQH010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.112"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(43..681)
                     /gene="plsY"
                     /locus_tag="LK694_002584"
     CDS             complement(43..681)
                     /gene="plsY"
                     /locus_tag="LK694_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBK5053402.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            808..1242
                     /locus_tag="LK694_002585"
     CDS             808..1242
                     /locus_tag="LK694_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBK5053403.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1408..3453
                     /gene="parE"
                     /locus_tag="LK694_002586"
     CDS             1408..3453
                     /gene="parE"
                     /locus_tag="LK694_002586"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBK5053404.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3465..5915
                     /gene="parC"
                     /locus_tag="LK694_002587"
     CDS             3465..5915
                     /gene="parC"
                     /locus_tag="LK694_002587"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBK5053405.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
BASE COUNT         2145 a         1038 c         1348 g         1515 t
ORIGIN      
        1 atttgtcaca ctcccgtttt atctctttgg aatgaaaaag tatcaggatt ttttcttgct
       61 gctattcaaa ccaaacggga ttcgactttc agtatggttt ctgatacgct tgatattgtc
      121 tttatgccga taaaagataa aggaagaaac taaaaaggca atgatcgtca gtaaccaatt
      181 gggcttagcc aaaatcacag gagctacata aggaaggacg atcgtagata aagtgatcaa
      241 tactgcactg atcatactgc tcaaactcac catacttgta caaaataaac taacgataaa
      301 gatgatacaa gaataaacaa aaaatacagg attaaatcct aaaagcattc ctgcacttgt
      361 cgcaacagct tttcctcctt tgaacccagc aaaaatcgga aatgtatggc caagaatggc
      421 gcatacccca aaccaaagag ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt
      481 agccaatgta ccttttaaaa tatccataag caggacaaca atcccggctg ttttgcctaa
      541 tacacgaaat gtattggtcg ttccagtatt accgctcccg tgttgtcgga tatctttttt
      601 gaaaaacaat ttgccaatcc agacaccgga aggaatagaa cctaaaagat atgctaaaat
      661 aagtaataag atcaatttca ttttttaccc tctttcaagt tgaaaactat cctttatttt
      721 agcatgaata ggtaacagga acaattgaaa aaatgaaaga tacaagtata atagatggaa
      781 taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt
      841 ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa
      901 cgaacaagtt acatggttgc acaggtattg caagacaatg gatataaaat cataccagta
      961 aatccattat tggctggtga agaaattcta ggcgagaaag tctatgcgaa tttgacagaa
     1021 atcgatgatc caatcgatat cgtagatgta tttagacgga gtgaattttt acctgaaata
     1081 gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg cacaattagg gattgaaaac
     1141 aaagaagcag ctgagatgct aagaaaagca ggacgtacag atatcgtaat ggatcgttgt
     1201 attaagattg aacttggtaa gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga
     1261 gatgatcgat agaggggaat gtcgaaaaag cggttaggag ataattaaaa ttgatttctc
     1321 gtctaccctc ttttttttca cgaccttttc tagtatgata atcgtggtgc ggttttgccg
     1381 caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag taaacaacga atacaatgac
     1441 tcgtccatcc aagtattgga aggattagag gcagttagaa aaagaccagg gatgtacatt
     1501 ggatccactg acagccgagg cttgcatcat ttagtctatg aaatcgtcga taatgcagtt
     1561 gacgaagcat tatcaggtta tggcagtgag attgatgtga ccatccatga agacaatagt
     1621 ataactgttg cagatagcgg acgtgggatg cctgtaggaa tgcatgcttc cggaatacca
     1681 acagttgaag tgatttttac tgtgctgcat gctggcggaa agtttggtca aggagggtac
     1741 aaaacttctg gtggactgca cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg
     1801 ctaattgtaa cgatcgttag agatggcgta gagtatcagc agaaatttaa aaacggcggt
     1861 aaacctgacg gtactttgaa aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc
     1921 cattttttac cagatgatac aatattcagt acaacgaaat tttcatatga aattttagcc
     1981 gaacggttaa gagaatctgc ctttcttcta aaaggcgtta aaatctctct ttctgatctt
     2041 cgtggagaag aaccagtcaa ggaaattttt cattacgaag aaggaatcaa agaatttgtt
     2101 gattatctga atgaagaaaa agatacattg acaccagttg tttatttttc aggagaaaaa
     2161 gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg gttattcaga aaatgtttta
     2221 tcttttgtca ataacgttcg gacaaaagat gggggaaccc atgaagctgg tatgaaagcg
     2281 gcgatgacga aatcttataa tgaatatgcg agaaaagttg gtttgctgaa agaacgggac
     2341 aagaatctgg aaggtagtga tttcagagaa gggttagctg ctgtactttc tatccgtgta
     2401 cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa aacttggtac gccagttgct
     2461 cgtaccgtag tagataacgt catcagtgaa caaatgggct tttatttgca ggaaaatagc
     2521 gaaatgagcc agatgttggt ccgtaaagca atcaaagcca gagaagcgag agaagcagca
     2581 cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta aaaaaggcga atctcttctt
     2641 tcaggaaaat tgacacccgc acaatcccga aaccctaaga aaaatgaatt gtatttagtc
     2701 gaaggggatt ctgctggcgg atctgctaaa caaggaagag acagaaagtt ccaagcaatt
     2761 ctacctttac gaggaaaagt catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa
     2821 aatgaagaaa tcaatacgat gatctatacg attggtgcag gagtcggtcc tgagttttct
     2881 atcgaagact gtaattacga caaagtaatc atcatgaccg atgcggatac agacggcgcc
     2941 catattcaag tattactgct tactttcttt tatcgttata tgaaaccgct gattgaagct
     3001 gggaaagtat atatcgcgtt accgccttta tataaagtat ccaaaggaca aggaaagaag
     3061 caagtcattg aatatgcgtg gacagatgac gaattggcag caatgattaa aaaagtcggc
     3121 aaaggatata tgctgcagcg atataaagga cttggagaga tgaatgcgga acaattgtgg
     3181 gaaacaacga tggatccgac ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg
     3241 caagctgaac gtcgagtaac cacactgatg ggtgacaaag tagaacctcg aagaaaatgg
     3301 atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca gtatcttaga taaaaaagag
     3361 gatactgaaa tctctccttc agtttctaat gatttattag atgaagaacg agcagataaa
     3421 aatgaaaaca atcagttatt tgaagtagaa taggaggtca cctgatggaa aatcgacaag
     3481 aagtacaaga attaacttta gaagaagtga tgggtgaccg cttcggacgt tattccaaat
     3541 atattatcca agaacgtgca ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc
     3601 ggatattatt tgccatgaac aaagatggca atacgtatga caaaggattc cgtaaatcag
     3661 caaaatctgt cggaaatatc atggggaatt atcatcctca tggtgaccgc agtatttatg
     3721 aagcgatggt ccgcatgagc caagactgga agttgcggga agtactgatc gaaatgcacg
     3781 ggaataacgg gagtatggac ggtgatccac ctgctgcgat gcgttatact gaagccagat
     3841 tatccaagtt aagtggagag atgctagcag atatcgagaa agaaacagtc gatctcgtgt
     3901 ggaattttga tgatacagaa aaagaaccaa cagtccttcc agctcgatac ccaaaccttc
     3961 tcgtcaatgg atcgacaggt atatctgctg gatatgcaac agaaatcccg actcataact
     4021 tagctgaagt gatcgatggc acaatctata tgatcgatca tccacaagcg agccttgaaa
     4081 aattgatgga atatattcct ggacctgact ttccaacagg agggatcttg caaggaaaag
     4141 atgaaatcaa aaaagcttat gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa
     4201 agatagaggc aatgaagggt aataaacagc aaatcgtgat cacagaaatc ccatatgaag
     4261 taaataaagc gaccttggtg aaaaaaatgg atgagatccg tttgaataag aaaatcgatg
     4321 gaatagctga agtacgagat gaaagtgata gaacaggtct gcaaattgtc gtcgaattga
     4381 aaaaagacgc caatgctcaa ggaattttga attatttgtt caaaaacact gagctacaga
     4441 tcaattacaa tttcaatatg gtcgctatcg accatatgac tccgcatcaa gttggactga
     4501 aagacatttt aagaagttat atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat
     4561 tcgatttagc caaagctcaa aaaagacagc atatcgtaga aggattgatg aaagctttat
     4621 cgattctgga tgaagtaatc gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa
     4681 agaatttagt cgacgtcttt caatttacag aagaacaagc agaagcaatt gtcacattgc
     4741 aattataccg tttgacgaac acggatatca cagagcttca aaaggaatcc gaaagcttga
     4801 ttgcgcaaat cacagagttg aataaaatcc tttcaaatga caaagagctt ttctctgtta
     4861 tgaaaaaaga attgcgagaa gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg
     4921 aagacgaaat cgaagaaatc aaaattgata cgcaagtctt agtggcacaa gaagacgtaa
     4981 ttgtcagtgt gacgcgagaa ggctatgtga agcgaacaag ccttcgttct tatagtgctt
     5041 ccaaaccaga agaaatcggg atgcgagaag gcgactactt gctatactca ggtgaattga
     5101 gtacattgga ccatgttctt ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc
     5161 atgaactacc cgatttgaaa tggaaagatg ctggtgagca catttctcaa acaatctcca
     5221 atctgtctgt agacgaatcg attttagctg ttttcccata tcaaaaaatt caagcagaga
     5281 aaacatttgt attcatcagc aagaatggat tgatcaagca aacacgaatg acagagttcg
     5341 aaccatggcg cacctataag agccgtccgc tcagctgtat gaagctaaag gcggaagaag
     5401 atgaactgat tgctgtgtat ctgaaagaag atcagccagg cttagatgtt ttcttagtaa
     5461 cgcatcaagg aatgggtttg cgctatcctt taacagatgt tcccgttgtt ggagcaaagg
     5521 cagctggagt gaagtctatg aatttgaaag atggagatag gattatcaac gggctgcttg
     5581 tgttagcaga aggtgatacg ccgatcgtaa tcgttactca gcgaggagct gttaaacgca
     5641 tgcttgctca agaaatcagc caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg
     5701 agctgaaaaa aaatccgcac cgtattatct atatgtcgga aggaagatca aaagaaataa
     5761 ccttgatgaa tcaaaaaggg cagcagttaa cgattgaccc aactgatttc cctataggtg
     5821 atcgaacatc gaatggatca tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag
     5881 ttatcgatgc tcctgagatt ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga
     5941 cgccatctgt attaatacag gtggcgtctc tttttcattc atttttaata aatccttata
     6001 cgtttgcaaa aaaagatgaa agtgttcaca atgcttaaat gttggt
//
LOCUS       DAFBQH010000107         5981 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.113, whole genome shotgun sequence.
ACCESSION   DAFBQH010000107 DAFBQH010000000
VERSION     DAFBQH010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.113"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            658..1392
                     /locus_tag="LK694_002588"
     CDS             658..1392
                     /locus_tag="LK694_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5053406.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1408..2577
                     /locus_tag="LK694_002589"
     CDS             1408..2577
                     /locus_tag="LK694_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK5053407.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2593..3588
                     /gene="lacD"
                     /locus_tag="LK694_002590"
     CDS             2593..3588
                     /gene="lacD"
                     /locus_tag="LK694_002590"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBK5053408.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3667..4593
                     /locus_tag="LK694_002591"
     CDS             3667..4593
                     /locus_tag="LK694_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HBK5053409.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4612..5862
                     /locus_tag="LK694_002592"
     CDS             4612..5862
                     /locus_tag="LK694_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5053410.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2007 a          976 c         1220 g         1778 t
ORIGIN      
        1 actgttgcca ttccaaagtt tgaaaaatgc tatttcgagg tagaaggata ttatataagc
       61 acggttgact aaacgaagtg ataataaaaa aagttattca agaataagaa aaattatgat
      121 acgaattcag ataaactgga gaatacagag acctttaaaa gggataagaa acaacataaa
      181 ttcctcttaa gagagaacga aaggacaaag ccagcaactt gatacacgag ctagtattag
      241 aggaatttat tctcatagac tcccttttga atacgtttca actttaattt tcatgttcat
      301 aaatgatttg aaaccaaagc aaatgcgttg cacttttcct ccgtcttccg aaaatcgttc
      361 aattcactgg taagtagtca tttgatagtt tgaggcaaat tgttgattga tgaagggtag
      421 gagctaaaaa aagataccag ttgaagagag aaaaagaaaa aaatagaggg tgttcttgaa
      481 tgaaaaaagt tggaaaatta tttttcattt ccttttattc ctattaaagt cagtgatgtc
      541 aatcatatta acaagactaa aaatgaaaat aaaaaaaaga ttgttcttag tattgactca
      601 taaaaaatac cactactata cttacattgt ggttatagcc agttaggaga gacatcgatg
      661 caaaatacct ttcaatcaaa atcactttat tatcagctgg tagaaatatt gaaacaacga
      721 atcgaaaaca gtatggtcgc tcatgacaag ctgccatcag aaagagagtt gaccagtttt
      781 tatggcgtta gtcgcacgac ggtgcgacta gcattgcaag aactggaaaa aatgggctac
      841 atttatcgac aacatggaaa aggaaccttt gtttcggaat taaggacaaa tgcgactgat
      901 ctagctggcg catatagttt taccgaacaa atgcgggcaa tgggcaaaca tcctgaaacc
      961 aagatattgg ccttcaagaa gattgaagca actaaatatt tagcgagtca tctgaatgtt
     1021 caactaggtg agcctttatt tcaattagac cgattgcgct tagctgatga tttgccaatg
     1081 atgatcgaga aatcctattt gccagtgaag ctatttatgt ccctttctca agaaattcta
     1141 tttctgaaac ctttatataa tttgttttca gagaatttca atcaaatcgt acggttggct
     1201 gacgaggaat tttatgccag cattgtccag aaagaagata gtgagttgct aaatattcca
     1261 gaaggatcac cagtgctcca tttagttcgc tcaacctata atctcgagaa cgaagtgatt
     1321 gaatttacat taagtgtagc gcgggctgat aaatttcatt ataagatcag gcatctgcgt
     1381 tccactaact gaattggagg aaagaaaatg tttatgttct caaaagaaga actacgaaag
     1441 ctaggagcag aaaatactac ccgagaaatt cgacagcaac cggaactgtg gcatgagacg
     1501 tttgaccgtt atcaagaaac agttgcagat ttagatcgtt tgtttacgga tgcgaaacaa
     1561 catgctggag agcgaccaat ccgagtcatt tttacagggg ccggaacctc gcaatatgtg
     1621 ggagatacac taattccgtt tcttactagt caaggaaata cgaaccagtt tatttttcaa
     1681 agtattggtt cgaccgatat cgttgctacg ccaaaagaat acctgattcc tgatgagaca
     1741 acgatcttgg tctcatttgc ccgtagtgga aatagtccag aaagtctagc cgcagttaag
     1801 atggccaatc agctggtcaa caagatctat catatcgtga tcacctgtgc accagacgga
     1861 ttattggcta cacaaatgcc tacaaaaaaa gaaaatctag ttttactaat gccaaacaaa
     1921 gccaatgacg ctggttttgc tatgacaagc agtttcagct gtatgacgct gactgcgcta
     1981 cttatttttg atcaagcaag caatcagcaa aaagaaagat atgttcatca attaagccga
     2041 ctaggagaag aagtcatcca acgagaatca gagatccaag ggtatctggc atcagacttt
     2101 catcgcatca tttacttagg ttcgggaagc ttaggcggat tgactagaga agcgcaatta
     2161 aaagttctcg agttaactgc tggaaaaatc gcaaccgtat ttgatacatc tttaggattc
     2221 agacatggtc ccaaatcttt tgtcaatgag caaacattgg ttttcctgtt tgtcagcaat
     2281 gactcatata cgcagaaata cgatgtagac atgctggaag aaatcaaaaa tgacggtatt
     2341 gcccaacgcg ttgttggaat tggtcaaaag ggttcattgt ttagtggtga ttcctttgaa
     2401 ttagttgcag attcgttact gccggatggc tatcttgctt tagcagatat catggtagcc
     2461 caaacgatcg cattactttg ttcggtgaag gttggtaata caccagatac accatcgcca
     2521 agtggaacag tcaatcgtgt ggtcaaaggc gtaacgattt atccatatga aaaataggaa
     2581 agagtgtgaa gcatgttaga atggtcggaa aataaaagac aagcaatgga acgtctatcg
     2641 acgccagaag gcattattac cgctttggcg atcgatcagc gaggcgcgct gaagaaaatg
     2701 atgtcggctt tagggatgga agcgacaaag gaaaaaattg agcagtttaa aacactaatt
     2761 tcccaagaat tgactcctta tgcctcagca attttattgg atcctgaata cggactgcct
     2821 gctgcacaga agcgtgcagc agcgagcggt ctgctgttgg catatgaaaa aactggttat
     2881 gatcaaaccg tacccggccg cttgccggat ctgctgtcgg actgttcagt tctgcgattg
     2941 aaagagttag gtgcggatgc ggtgaaattt ttactgtatt acgattatga agaagcagag
     3001 gaaatcaatc ggcaaaagca agtttttgtt gaacgaattg gaagcgaatg catggctgaa
     3061 ggactgccat tttatttaga gttattgtca tatgatgcta aaaatgtaga tacgaaatcg
     3121 ctttcctatg ccaaagcaaa accgcgcaaa gtgatcgaaa tgatgaaaga attctcgaaa
     3181 acccagtata aagtagatgt cttaaaggtt gaagtaccag taaacatgtc atttgttgaa
     3241 ggaaatgcaa caggggagat cgcctataca cgtcaagaag ctgccgctta ttttaaagaa
     3301 caaagcgatg caacagattt gccgtatatc ttcttaagtg ctggggtatc agcggcatta
     3361 ttcatggaga cattgcgttt tgccaaacat gctggggcac gattcaacgg tgtactctgt
     3421 ggcagagcaa cttggaaaga tgcagtcgct cctttcgttg aaaaaggaga agcggcaact
     3481 ttagaatggc tgacagaaca agggacacaa aacattcgtc aattgaatga agtgataaga
     3541 gaaactgcca tcccatggta tgagaaagta agtgaaagca cttgttaatg tttctaatat
     3601 attagtagtg ataagagata tgtgtgatta taatttatca ttattgaaac tgggaggata
     3661 ttttatatga ataaaagatt gttagattat tcagcttcag attttttgaa agctaagccg
     3721 atggaattaa agcaggcaat tttggcttct gaaggtagaa caattatggc agaaaatgtg
     3781 ccaagtagct gtccttacat gggaaaatca gtgacaaatg cagaattgga gagagctgct
     3841 ggagctgatt taattttgtt taatgcacta gatcttttta atcctcaaat ttcaggtgta
     3901 cctttaactc taaaagagaa tccaataatc tggattaaaa aagctttagg aagagctatt
     3961 ggtgtaaatt tagaacctgt agacaaagaa gttttgatga gtgaaaatcg aactgaaatt
     4021 tcaagtggta gaatcgtttc tcctgagact ttaaaaaagg caaatgaatt aggctttaat
     4081 tttatttgtt taactggaaa tccaggaaca ggagtatcta atagaactat tgagaaagca
     4141 ataacattat ccaaatcaat ttttaatgga ttaattatcg ctggaaaaat gcatggtgct
     4201 ggtgttgatg aaccaattat gaacttagat atagcaaaaa gatttattaa aacaggtgta
     4261 gatattttat tagttcctgc tccatacact gttccgtatt tcatggaaag tgatttgcga
     4321 ggaatcgttg attatataaa agattttaat agaaataaat ctattgagga aaaagttcta
     4381 gtactaacgg ctaatggaac aagccaagac tcttctgacc aagaaactat taaaaaaatt
     4441 gcattagcat caaaagcatg tggagctgat attcaacaca taggggattc tttaaatggt
     4501 atttgtcttc cagaaaatat ttttgctttg ggtcaagcaa ttcggggata tcgtcatcaa
     4561 attattatgt taggaaaatc taattatcgt taattaagga ggaaatgaaa gatgatcgac
     4621 aaagttcaaa aaatagctgc aaaaattagt aatcaaagac atctactagc tttaagagac
     4681 agcatggctg cagttatgcc attaatcatt attggttcct tttttatgtt attagcaaat
     4741 ttcccgatag aaggatttac aaaatggtta tctgaaattg gagtacaatc ctttttgaac
     4801 aaagcatcag attcaacatt tggtattatt ggattagctg ttacctactc tttgggacac
     4861 aatctagcaa agacttatgg tgtagactca ttatcagcag gattattatc tttgagtgga
     4921 tatgttttac ttacaccttt attggatgca gatggttcta taggatttcc aacaaaatac
     4981 tttggtacct cgggtctttt tgttggtatt attgtagcac ttgtatctac agaaattttc
     5041 cggtggtttg tacttaaaaa tattacaatt aaaatgccgg caagcgttcc accaaatgta
     5101 agccgagctt tcgttgctat tattcctggc ttttttgtcg ttctactatg gtttattgtt
     5161 gttgtaattt gttataaact tggaattgaa aatgttcatg ctcttattgc agatacactc
     5221 gcaaaaccac tatctttact gacgaaaact ttaccaggaa ttatattggt tatacttatt
     5281 caatgtttct tttggatgtt tggtattcat ggtgcacagg taacggggcc aataattgaa
     5341 ccgttattat tgcaaaactc tgatgtaaat agaattgctt atcaagctgg aaaagaactt
     5401 cctaacatta taacttatga attcttatat aactttgttt tttctggtgg tgcaggttgt
     5461 ttatttgcac ttgccatttt aacatttttc ttttcaaaga gtcaggaaaa taaaacttta
     5521 ggtaagttat caatcgctcc agtaagtttt caagtagcag agccccttct atttgggttt
     5581 ccaactatat taaatataaa aatgttaatt ccatttgtaa ctgcaccagt agtaacaaca
     5641 ttaattactt atttttcaat gagtatggga ttagttgcaa aacctgtagg agcaacaatc
     5701 ccatggacaa ctccaccgat aattgccgga tttttagctt ctggtggacg aataagtggt
     5761 gcagtgattc aagtaataac aattgcaata aatgttttga tatactatcc attctttaaa
     5821 ttagatgata atgccaagct aaaaagcgaa aaaaatgatt agaactacct atatgtttct
     5881 aactgtagtt aagtatcgcc gtttgtataa ttaagcgaga caaataaaaa agccatttat
     5941 gttacactca aattgtaata cccgctaaag catacaagga g
//
LOCUS       DAFBQH010000108         5907 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.114, whole genome shotgun sequence.
ACCESSION   DAFBQH010000108 DAFBQH010000000
VERSION     DAFBQH010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.114"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(95..667)
                     /locus_tag="LK694_002593"
     CDS             complement(95..667)
                     /locus_tag="LK694_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK5053411.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(674..1336)
                     /gene="rpe"
                     /locus_tag="LK694_002594"
     CDS             complement(674..1336)
                     /gene="rpe"
                     /locus_tag="LK694_002594"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBK5053412.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(1352..1867)
                     /locus_tag="LK694_002595"
     CDS             complement(1352..1867)
                     /locus_tag="LK694_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBK5053413.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(1869..2153)
                     /locus_tag="LK694_002596"
     CDS             complement(1869..2153)
                     /locus_tag="LK694_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5053414.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(2187..3515)
                     /locus_tag="LK694_002597"
     CDS             complement(2187..3515)
                     /locus_tag="LK694_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBK5053415.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(3529..3984)
                     /locus_tag="LK694_002598"
     CDS             complement(3529..3984)
                     /locus_tag="LK694_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5053416.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(3987..>5907)
                     /locus_tag="LK694_002599"
     CDS             complement(3987..>5907)
                     /locus_tag="LK694_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK5053417.1"
                     /translation="KLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLEDYVVNYQVTI
                     ERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFGDKPVSLTHL
                     SQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAIKQLVFYYAN
                     DMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLISTLVIQCNRLL
                     KGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLCRQLFAHRVT
                     NNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLRLKKGIKIQN
                     PLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIEDQSKANNII
                     IICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISSLSFDAGGIP
                     LVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKFVQSSLVKTG
                     VQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALPHANPSMVIE
                     SNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQKEMVDRLLQV
                     HDSEKLLRIFQQKGE"
BASE COUNT         1919 a         1147 c          836 g         2005 t
ORIGIN      
        1 aattattaag ttatgtctaa tcaaataaaa aaaccattct catatttgct gaataattag
       61 catttagaga atggtttttt atctttaaaa tctactactc aaaatctgca tgtcgagcaa
      121 acatcgtttc tgagctttca cctgttttat ccattaaaga taacactaaa ctatcaaagg
      181 tgagaaatgc taattgttca aaacttgaac ccataggttg tgaaaaagta tcttcttctc
      241 gttgattttc atctttggta atacctggta atacaagaac agaatctgct aattgaccaa
      301 tcgacgagtc ttttttcatg gtaactaaag caatgcttac accactttga tttgctttgg
      361 ccgctaaatt ttttaaacta cctgtttcac ctgagccaga acagataatt aataaatctc
      421 caggttgcga atgtggtgaa ctaatttcac cgacaaggct gactgaaaaa cctaaatgca
      481 ttaagcgatt cgtaaatgct tgcatggcaa ttccagagcg tcctgcacca gctagaaaga
      541 tgtgtttagc ttgaacaatt ttctcactaa attgttctaa ctctacagaa ttaatacgtt
      601 ttgagttatt ttgtaattct aagataatcg cttctaaata gtttttattt tggtctttat
      661 tggtcatgat cttttacttc acttcctcta atgattgatt tagtttatca atttgttctt
      721 gcggggtatt tttcccattg aagatgaaac cgcctgatac aaaaatatcc gcccctgctt
      781 tagaacaaac tttaatcgtt tgattatcaa tcttgccatc tacttcaata tcatacgtat
      841 aatcattttg tttgcgtatt acatctaact gttcgatttt aactactgtc tcttgaataa
      901 aatgcttatt ctctgttcct ggattgattg tcattactaa tacttgatcg actaaagata
      961 atactggttc aataaaactg actggtgttg caggattaaa agtaatccct acttttgctc
     1021 ctgcttcctt gactaactga atgaccttca ttgcgtgttt ggtagcttca atgtggaagg
     1081 taacaatatc tgctcctgct tctacaaatc ttgtgacaat tttttctggt gtatcaatca
     1141 tcaaatgaac atcaaacaca acgtctgtta gtggacgaat atctttaata acacttggtc
     1201 caaaagccat atctggtaca aaacagccat ccattacatc aatatgcaaa taatcaacat
     1261 tcttttcttt taagatttca aataactctt taacttctgc gataggtgca tttaacaatg
     1321 atggagcaat tgtcattctc tattcccccg tttattctcc cataatcatg agattgattg
     1381 ttctagttcg ctctcttagt tgatcccaat cgacaaaata gacacggccc atttctccta
     1441 ataaaagttt accttcttga acaattaaag tcattgagtt ggttccataa atagatgaac
     1501 gtaaatgtgc atcggtattt agcatgaccc atttttctgc agggtaattg ggatcagata
     1561 atcttgttcc aaaatcaatg tgttttggcc ccggactgtt gtattgattt tcactcgtca
     1621 tttttggaat aatcgtttcc attacattgt tgatgtctac atgtaaataa tcatcaccaa
     1681 aaaagtttgt gtcatgcatc gtttcttcaa aaaagagtga gcaagttgta tgcgggctcg
     1741 aaattactaa cattccattc tcaatacctg ctgtatcaac tgtatctaaa agttcttttc
     1801 gaatatcatg atacgaagga cgccctgaaa ctgtttcaat agtcaaagtg tcatgatgaa
     1861 aacgcatatt aaacacctaa tctttctttt acatctgcat aaatggcatc tgtaccaata
     1921 cctgttaata agcccaagcc attcgttact ttagctttaa ttacatcagg gacaattgtt
     1981 gtacttacta cagcatcata ttcatcaatt ttatttagct catcagcaat tttagattga
     2041 taaattttca cttgatctgt taatccttct tcttccaacc attttgataa ttttgctact
     2101 gcaattgtag aagtagcatg acctgctcca caaataacta ataatttttt catattgatc
     2161 tcttctttct tattttaatt ttatgattaa gctttcttca ctttgttcat gtagatagaa
     2221 gcacctacta aaacaactcc taatccacct attccaatta caccaaagta agatgctaat
     2281 aaaacaaaca accctactgg ccataatcca cataacaaca tagttactaa tcctgctgtc
     2341 cccacatcga aacttgctaa tgtaaacaca tcagtgataa ttggtgccaa ccatgttgcc
     2401 atatataatg ttgtaacaac atacaacgaa catcctacta atgatctcac aatatttcct
     2461 ttaaaataag gaatcattaa acagatggcg aaagcaaaga atgctaaatc tccaaaaggt
     2521 aatactttat ttcctggaag aattagtgct agcaataatg agattggtac aagcaataga
     2581 gaagttgaca taatagttgg atgacccaca gttaacgctg catccattcc aatgtttact
     2641 tggcgaccat ttaacttttt attcgtaaat tctttcgccg cttctgcaat tggcattaaa
     2701 ccttccataa acattgctac cattttaggc ataatcttca tcactgccgc agtcataatt
     2761 cctaagtttc caattcctgc tacgtcatag cctgccataa tacccactac aataccgata
     2821 attaggccaa taacaactgt gtcacctaaa acaccgaaac gttttgttaa tgtttctgca
     2881 tctgcatcta ttttattaaa tccaggaata cgatcaaaaa cccaatttac tggtttggca
     2941 aatagtactc ctgataatcc catcatatgt gtgatagaga ttcctggata gccgaaaaat
     3001 tcgctaacag ttttttgtgt acgatctgct aagaaccatt gtaaaattac tgcgacaatc
     3061 gcgccaatta agccaaacca aaagtttccc gtaacagatt gagtaatcat acctaggaaa
     3121 attgcgaacc aaaagttcca aatatcaaca ttcaatgttt ttgtccaacc caaaaccaca
     3181 aatatgaagt taagaattaa tgaaattgga atcattaaaa ttcccaatga tgtagagtaa
     3241 gccaaaggtc caccaacacc tacgccaata tctaggatat tcaggccacc accgtattgt
     3301 tcccccattt gttgaattgc tgttcctaaa ccatctgata ataaacttaa aactaatcct
     3361 aaaccaatag aaccgatacc aacagtaacg ccggaaacaa atgctttacc aggttttaat
     3421 ccaaataata tcccgattac aaaaataatc agtggtaaaa agacgtttga ccctaatcca
     3481 ataaaccatt caaaggcatt taatacaata tccatctctc tattcctcct aataaactcc
     3541 tgcagaatgt actaactcaa taaatgcttc tttcgtggta catgcaagta attcatttac
     3601 tttttcttca ttttgaaata ggctaactaa gttttgtagt gtatcaattt gttcatgagg
     3661 ttgtgacatt gccaacataa agactaaacg tacaggtatt tcatgagatt tatctgtcat
     3721 atctgaaaat accagtggtt cctttaatga ggcaaaacag atttgagagc tatttacatg
     3781 tgcggaatct gtatgtggga tagcaaaccc aatttgattt acttctaatc cagttggaaa
     3841 ttcttgttca cggttggtga taccttctaa gaaactatct ttcacgcaac cagcgttttt
     3901 taattgttca gacattactt gaaacaattc tttttgatct ttcacttcta attgaaataa
     3961 acataattct ttatctagat acatttttat tctccttttt gttgaaaaat tcttagtaat
     4021 ttttcgctat catgaacttg taacaagcga tcgaccattt ctttttgccc aataaattgg
     4081 taaagttctt catatactcc tctaatctca gatacatctt cttcagctaa actaaccata
     4141 atgactaatt tgacttttcg atcgccccaa ttaatcgcat tttttaatgc tacaataaaa
     4201 atgttggatt caatgaccat tgatggattc gcatgcggta aagcaacacc actttctaaa
     4261 ttagtattcc ccattttctc tctagagaag atagaagcac gaaactcttc agtaactaat
     4321 gaattggctt ctagttcgga aatcatcaaa tccaaacatt cttctttgct ttccacctgt
     4381 actcctgttt ttactaaaga tgattgaaca aatttagata tggtgggaac tttaaccgct
     4441 ttaggaatca gttcattttg aataaatcgc gaaggttctt ctattaaata tttagtataa
     4501 gcttccataa tattcatata atctgtttta cttaccaatg gagataccct tactaagggt
     4561 ataccacctg catcaaagga aagagaggaa ataatcaaat caacttcccc aatttcagct
     4621 aaatgaaact tatctaaagt tgatatttct acattatctt ttgctggtaa aaaccgtcga
     4681 actttattgt aaattaattg cgcagaagaa ataccgtatt ggcaaataat gatgatatta
     4741 tttgctttgg actgatcttc aatagcaatc tgaaaatgaa tcaaaattaa ggaaacttca
     4801 tcgtccgtta aagtcacatc aaattcttct tcaaggatgg acatggcgta ccatactatg
     4861 ctaaagagtt ctgaatattg agatttgata ttttctaata acggattttg aattttaata
     4921 ccttttttta aacgaaggac cattgctggc acatgataca ataaggattg atataatcga
     4981 tcattttgag tcaaatcaat tttttcaatc tcactcattc tttcaatcaa tttatgaacg
     5041 ctcatagcgt actcttcatt gctggttttt aaattatttg tcacacgatg tgcaaatagt
     5101 tgacgacata gatattcttt atctgcgtca taaaatggaa gattcaattt tgccttcaat
     5161 tcttcaatta aaccatttgc cacaatgtac gtttccatat agcgaatgct attgaagagg
     5221 aattcttctt caacagttaa atgatgacct ttcagtaaac ggttacattg gataactaaa
     5281 gtagaaatca atgaccgaac atagtaatca gaaacctttt cagtcagttc actgtaatca
     5341 ttgaatagca aatcatgaat gacactaata acttttgaat caaacaacat atttaaaact
     5401 tgaaaaaatg tttttgattc catgtcattc gcataataga atacaagttg cttgattgct
     5461 cgttgaattg cttgttcatt accagtaaaa ctaattcctt ctgcagatga ctgcaactta
     5521 acttctccac tgccaataat tttattgata attctcaggt cattgtataa cgatgtttta
     5581 ctaaccataa aactttgaga caaatgagtt aaagaaacag gcttatcacc aaacaaaact
     5641 tctttaatga tctcgacacg tcttttttct ggagaataaa tatctttttc aagataattt
     5701 tctaccgatg aaatcaaatg ttcaacgttt ttcctatcac ctatgagttt gatacccaaa
     5761 gaaggtaatc gctcaattgt tacttggtaa ttaactacat aatcttccaa caattcaata
     5821 tcttgataaa tggtcttggt cgacactgcc aatttatcac taaaaaaaga agcgggttta
     5881 aagtcttctt gtcgacttaa taatttt
//
LOCUS       DAFBQH010000109         5708 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.115, whole genome shotgun sequence.
ACCESSION   DAFBQH010000109 DAFBQH010000000
VERSION     DAFBQH010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.115"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..194)
                     /locus_tag="LK694_002600"
     CDS             complement(<1..194)
                     /locus_tag="LK694_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053418.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFE"
     gene            complement(737..2887)
                     /locus_tag="LK694_002601"
                     /pseudo
     CDS             complement(737..2887)
                     /locus_tag="LK694_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3033..3608
                     /locus_tag="LK694_002602"
     CDS             3033..3608
                     /locus_tag="LK694_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5053419.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3822..4517
                     /gene="vanR"
                     /locus_tag="LK694_002603"
     CDS             3822..4517
                     /gene="vanR"
                     /locus_tag="LK694_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBK5053420.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4495..5649
                     /locus_tag="LK694_002604"
     CDS             4495..5649
                     /locus_tag="LK694_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBK5053421.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1784 a         1096 c         1083 g         1745 t
ORIGIN      
        1 tgttcaaaat ctaaaatcaa cacatcatgg ggagtgtgat aataaatatt ttcccttaat
       61 gtaccaacaa tggtaattcg gtcaattgac cattgcattt ttaagggggt gcacatagtt
      121 tgtgcattta accgcctgtt agaaatacgg ttaagttttt cccactcatt tacgattatg
      181 ccttttttac tcaaaaatag ctcctcctca acgaaaaaag agattcttgg aaagaatttc
      241 cagagttcac gtaactctaa aaatccacat ccaaaaatct ctagcttgaa gaaacctaaa
      301 aatattcgta taatgaactt gagttacgga actaggtgaa gtagtttccg taaaggtcga
      361 gagtgcgtca acactgctcg actatttttt tactcttttt tctcattttc tttgattata
      421 gcactcctct agaaaaaata aaaagacctc agggaatttc attccccgaa ccccttgagc
      481 ctcctcctgc cgaaatagaa tggcgctgcc agcatttatt tcgttcagct atcggctaca
      541 caatctaaca cgaccgttcc gctaccagtc gccttacagg ctccattttc gctcctctaa
      601 cgtgtttcac cgattgtgcc gataactaaa cgaaataaac gctaaaaccg ctcagaacgc
      661 aaaatatgcc attttagagc tattgtagaa aatatagttt gaaatgcaat tttcttcttt
      721 cagcttttta tttcccactt gtaaatcggt agatggtggc tccttttcca gttccataat
      781 gtggatttgc atctgcatgt agtgatgaaa cacctagctg cattctcata ccatctgacg
      841 aagatgttgt accgtcgccc caatagaaag gcaattgtaa tttatgatga aagtttacta
      901 atatggcttg ggctttattc atggcatctt catacatgcg ccattgagat acattggcta
      961 gttgcttata tgtaagtccg ggtgtggctt cggccatctt gctcaagcca atattcattc
     1021 ccattcctaa aagggcagcc atgataatga ttgtttcttc cttatctggt tttcgattat
     1081 tggaagcatg agtgaattgc tcatgaaatc ctgttatatg ggccacatcc atgagtaaat
     1141 cagttaattt tattcttggt agcatctgat aaaggcttgc actaaatttt tttgcttctt
     1201 ctggaacatc tttttctaag cgtgcaagtg atagctttcc tttttcaaga gaaaccccat
     1261 ctaacttatt ggaattggca gctaaccact ttaacctttc attaaagctg ctggttctct
     1321 ccgttatata atcttcgaat gataaactaa ctgataatct cgtattcccc ttcgattgat
     1381 tccatgtatc ttccgaaaac aaatattcct caaaatccct atattgtctg ctgccaacaa
     1441 tggaaacatc tcctgcccga acatgctccc gaagttctgt taaaacagcc atttcatagt
     1501 aatgacgatt aattgttgta ccatcatcct cgtataaatg tcttttccat cgttttgaaa
     1561 taaaatccac aggtgagtca tcaggcactt ttcgctttcc agattcgttc attcctcgga
     1621 taatctcaac agcttgtaaa agtggctcat ttgcctttgt agaatgaaat tccaatactc
     1681 ttaatagcgt tggcgtatat tttcttagtg aataaaaccg tttttgcagt aagtctaaat
     1741 aatcatagtc ggcaggacgt gcaagttcct gagcctcttc tactgaagag acaaaggtat
     1801 tccattcaat aaccgattct aaaaccttaa aaacgtctaa tttttcctct cttgctttaa
     1861 ttaatgcttg tccgatgttc gtaaagtgta taactttctc atttagcttt ttaccgtttt
     1921 gtttctggat ttcctcttga gccttacgac cttttgataa caaactaagt atttgcctat
     1981 catgaatttc aaacgcttta tccgttagct cctgagtaag ttgtaataaa tagatggtta
     2041 atatcgaata acgtttattt tcttgaaagt cacggaatgc atacggctcg tatcttgagc
     2101 ctaagcgaga cagctgcaac aggcggttac ggtgcaaatg actaatttgc actgtttcta
     2161 aatccattcc tcgtatgtat tcgagtcgtt ctattatttt tagaaaagtt tcgggtgaag
     2221 gatgacccgg tggctctttt aaccaaccca atatcgtttt attggattcg gatggatgct
     2281 gcgaggtaat aatcccttca agcttttctt tttgctcatt tgttagagat ttactaaccg
     2341 tattaaatag cttcttttca gccattgccc ttgcttccca caccattctt tcaagtgtag
     2401 tgatagcagg cagtataatt ttgttttttc ttagaaaatc tatgcattca tgcagtagat
     2461 gaatggcatc accattttcc aaagctaatt gatgaaggta cttaaatgtc attcgatatt
     2521 cactcagggt aaaagttaca aagtcgtatt cacttcgaat ttctttcaaa tgatcccaaa
     2581 gtgtattttc cctttgagga taatgatcaa gcgaggatgg actaacacca atctgtttcg
     2641 atatatattg tatgaccgaa tctgggatgc ttttgatatg agtgtatggc caaccgggat
     2701 accgaagaac agctaattga acagcaaatc ctaaacggtt ttcttccctc cttcgcttat
     2761 taactatttc taaatcccgt ttggaaaaag tgaagtaggt ccccagtatc cattcatctt
     2821 cagggatttg cataaaagcc tgtctctgtt ccggtgtaag caattctcta cctctcgcaa
     2881 ttttcattca gtatcattcc atttctgtat tttcaattta ttagttcaat tatatatcaa
     2941 tagagtgtac tctattgata caaatgtagt agactgataa aatcatagtt aagagcgtct
     3001 cataagactt gtctcaaaaa tgaggtgata ttttgcggaa aatcggttat attcgtgtca
     3061 gttcgactaa ccagaatcct tcaagacaat ttcagcagtt gaacgagatc ggaatggata
     3121 ttatatatga agagaaagtt tcaggagcaa caaaggatcg cgagcaactt caaaaagtgt
     3181 tagacgattt acaggaagat gacatcattt atgttacaga cttaactcga atcactcgta
     3241 gtacacaaga tctatttgaa ttaatcgata acatacgaga taaaaaggca agtttaaaat
     3301 cactaaaaga tacatggctt gatttatcag aagataatcc atacagccaa ttcttaatta
     3361 ctgtaatggc tggtgttaac caattagagc gagatcttat tcggatgaga caacgtgaag
     3421 ggattgaatt ggctaagaaa gaaggaaagt ttaaaggtcg attaaagaag tatcataaaa
     3481 atcacgcagg aatgaattat gcggtaaagc tatataaaga aggaaatatg actgtaaatc
     3541 aaatttgtga aattactaat gtatctaggg cttcattata caggaaatta tcagaagtga
     3601 ataattagcc attctgtatt ccgctaatgg gcaatatttt taaagaagaa aaggaaacta
     3661 taaaatatta acagcctcct agcgatgccg aaaagccctt tgataaaaaa agaatcatca
     3721 tcttaagaaa ttcttagtca tttattatgt aaatgcttat aaattcggcc ctataatctg
     3781 ataaattatt aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta
     3841 ttgtggatga tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt
     3901 atacggtttt caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga
     3961 ttgaccttgc catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa
     4021 aaataaggga caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag
     4081 ataaaattac agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac
     4141 tggagttaat tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa
     4201 aggagcagaa cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg
     4261 agtgttatct gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa
     4321 tcctctgtga aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg
     4381 gcgacgaata tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg
     4441 aaaaaatgaa cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt
     4501 ataaaattga aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat
     4561 atcgttgcaa ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga
     4621 gggaaacttg gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg
     4681 gacgcgatga aattatatca atattccata cggaacaata tagatatctt tatttatgtg
     4741 gcgattgtca ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac
     4801 tttgacgaga taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag
     4861 ctttctgcgg aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa
     4921 aagcgagagc aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg
     4981 gcgcacgata ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag
     5041 gctccagaca tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg
     5101 tatcgactcg aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg
     5161 ataacgctaa caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa
     5221 ttttatcctc agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg
     5281 accgtgtccg gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc
     5341 gctgcataca gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg
     5401 gtgtcaatcg aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt
     5461 gaaaagttct ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga
     5521 ttggcgattg caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat
     5581 gataactata cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg
     5641 aggtcctaag agatgtatat aattttggct ctttgtcaaa tgatgttggt aaagagaaat
     5701 cttagaat
//
LOCUS       DAFBQH010000110         5480 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.116, whole genome shotgun sequence.
ACCESSION   DAFBQH010000110 DAFBQH010000000
VERSION     DAFBQH010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.116"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..467
                     /locus_tag="LK694_002605"
     CDS             <1..467
                     /locus_tag="LK694_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053422.1"
                     /translation="SYNSETDPASVSVTEAKKIVAEKFKNDIETTPIMPRGAFPPDVG
                     TSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFLRWKSYIDDGRVGNVNQA
                     NATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNPVAGTYYVVQNI"
     gene            592..2943
                     /locus_tag="LK694_002606"
     CDS             592..2943
                     /locus_tag="LK694_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HBK5053423.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3023..3418
                     /locus_tag="LK694_002607"
     CDS             3023..3418
                     /locus_tag="LK694_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053424.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            3743..4360
                     /locus_tag="LK694_002608"
     CDS             3743..4360
                     /locus_tag="LK694_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5053425.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            4401..4700
                     /locus_tag="LK694_002609"
     CDS             4401..4700
                     /locus_tag="LK694_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HBK5053426.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            4734..4901
                     /locus_tag="LK694_002610"
     CDS             4734..4901
                     /locus_tag="LK694_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK5053427.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(4946..5470)
                     /locus_tag="LK694_002611"
     CDS             complement(4946..5470)
                     /locus_tag="LK694_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5053428.1"
                     /translation="MDKLFQEKASGKDTERPEFQKLLNYIREGDCVIVTSLDRLGRDY
                     EDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSLLSYIAQNEREKIR
                     ERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQGIPVAEIAKENGI
                     SRPTIYKIKKANDL"
BASE COUNT         1862 a          778 c         1097 g         1743 t
ORIGIN      
        1 gttcatataa ttcagaaact gatcctgctt cagtttcagt gacagaagca aaaaaaattg
       61 ttgctgaaaa attcaaaaat gatattgaaa ctactccaat tatgcctcga ggagcatttc
      121 cacccgatgt tggtactagc ggacaccttc ttgtactagc aggaggagga agctataaat
      181 cacagccatt tagtggtgcg ggatggcgtt ttggaggtat gggtgtaaaa ccagctgatg
      241 gtacaggcaa ctttttacgt tggaaatcct acattgacga tggaagagta ggaaatgtaa
      301 atcaagccaa cgcaactaaa tctggttcta ttcaaggaat ggcattgtat gctcgtgaag
      361 gattcaaata ctttgcacca aacgggcctg gttggacaat gaatataggt atttattaca
      421 cttataatcc tgttgcaggt acgtattatg ttgttcaaaa tatttaataa aacagcacaa
      481 agagatttct ttgtgctgtt ttattatttt tagttgttga caaacactaa aatataaaat
      541 ataatcaaca cataaacgaa cgtatgttcg gcaagaaagc aggggatttt tatggatgag
      601 atggttttaa gtactcagaa atggctaaac aagaaataca gtaatgttac agggtttgat
      661 aaggttccag aaaatgggcg aacagggtgg cctacaatct atggacttat tgaaggacta
      721 caagtagaac ttggtattac taatctagta gctaattttg gacctaccac tgaaaaaatg
      781 tacgataatc aggttactcc taaatggggc aaaaatctac ctaaaaatat tgtatttctt
      841 attcaaggtg ccttttggtg taaggggatt aatccaggtg gcttcgatgg ggtatatact
      901 ccatatttag atactgctgt aaaagagttg cagactgatg cagggtttaa tggaagcgct
      961 gtaactggag ttttggatgc gaaatgggcc aaagcgttat ttgatatgag tgcttttgtt
     1021 cttgttcccg gcggagatag taaaattaga aaaatgcagc aatggttaaa tgtaaattat
     1081 ttagaataca cgggaataat gccttgtgat ggtatttatc aaagaaattc aaatcaagca
     1141 ttaatttttg cattacaagc tgaactagga tattctccca gtgaagcaac agggtcttat
     1201 ggtaatggga caactagtaa aacttatcct gtgtctgaag gtaactcggg aaactatgta
     1261 agaataattc aatatggatt atatgttaat ggttattttg aagacggtac attcgatggt
     1321 attttcacta aatatatggg tttagaagta ctggcattcc gaaaatttat ggtattgcct
     1381 gaatatactg aaatagcaga tgttgttgta ataaaaggac tattgtctag cgctggagat
     1441 attagaagat ctgcagatgg cgcagatact tctacgcaat taactagatc tcaaatccaa
     1501 acattggttg ataatgatat aaaaatagtt ggtagatatc ttactggtac tgttggaatt
     1561 ggaaaagatg aacgaaataa gtatctaact acagaggaat tgaataatat tttttctaaa
     1621 aatttatctg tgtttcctat ctatcaagat ggaggtgcag cattagctta ttttacttat
     1681 gatcgtggct tatcagatgg taagaaggct atagatgcag caaaaaatct aaatatacca
     1741 ttaacaacag ttatttattt tgctgttgat ctagacatgt taggggaaga aattcttgca
     1801 tatgctggag aatattttaa gggggtatct gctgtaatgt catatagcgg gtatcaaacg
     1861 ggtgtttacg gaacaagaaa tgtttgttct caaataatca ataacgggta tgcttcgttt
     1921 gcatttgttt cagatatgtc gactggttat agtgggaatt taggttttcc tatgcctaga
     1981 caatggtctt ttgatcaatt tgttgaattt accataggaa gtggcaacgg agctgttggt
     2041 atcgatttaa tcgctacatc gggtagagat agcggattta atgagttatc aaatgatact
     2101 aataatgata attacattgc caaatacaat cagaaggtta taaatcagat ggtacgtgcg
     2161 tatcaatatc tatcacaagc tggtcttgat aatccttgga accctcattt gacattttat
     2221 agatatgtaa attattctgg tttgtcatgg gacatcattt cttctcctgt aacagagcat
     2281 gatagaaaaa tttatgatga atataggaat aaattaacta atagcgaggg cctgtataat
     2341 tattttatag atcctaatac tggttcaata ataggtttgc ctcacttaat tgtgacgtta
     2401 caatcacaga tgtttctagc cgacacaatc aatgatagtg tttctgactt tgcaggttgg
     2461 ttaggagatt taatgacttg ctggggtgaa gttaagaaat taggaataca aatggagcaa
     2521 ggtgtatttg cattagtagg tacggctgca ggtggaacat ttagtctaga agatttatac
     2581 caagatgttg acgcggtaaa tgtttatacc ttagcaaaaa gaaattctga ttcaacagct
     2641 ttatcggctt taaaaacgta ctattgtgat aagggtgcta ttgatagatt taatcaattt
     2701 gttaagaaca gatttggttc ttcagataaa atcgcttcag taacaaatag tttattagaa
     2761 aatgacgaaa cagttgatat cttaggtgta gggataattt catttttcat tgataaaata
     2821 gttaataatt cacttgggac agattttact tatcttgatt ttattggaga tgaggggaaa
     2881 gttgcaaatg gattttctga aaaaattatt caatttcaag aaaatagaag taaacttaat
     2941 taagaatcgt ttactattta aacagtgtcc ttttcctttt taaatatata cgataaaata
     3001 agtatatgaa ttgtaaaagg acatgatgat tatggaagaa atagaatgga ataatctgac
     3061 tcaaaataat aaaaataatt ctggatgtgg aattatcttt atgtttgtta tagttttggg
     3121 aatagtagta cttttaataa ctgtcaagat agtagattac tttgcgaata ctcctgaaga
     3181 acaaatacag agaaaacttc ctgagtttga aagagtacca ccttatgatc caagagataa
     3241 ttcatttcaa gtaaaaaaat taaaagagtc tccaaatgaa atgtggtttg aagttacagt
     3301 tgaatcaagc gacttcaaag aaaaaaagaa agaatacaat tatattgtaa aggcaaaagt
     3361 atcaaaaaga tttattttta accctaaatt agaaatatat agtgttgtaa tagaataata
     3421 gtgttaaaaa aattgtaaaa gttaaagaag tcaaattctg atgtgaaaaa taaaatatac
     3481 catggaagac ataaaagaaa aactggataa ctagtttatt taatggcaga ccgttaaggc
     3541 ctgccatttt tatgtatttt agcggaataa atataatcat gcgggagtgt aaacttactg
     3601 tttcaaaaag tatacttttt gacatgcctt gattttcttt gtttatgtat ggcataataa
     3661 ccacataata caatttgaaa cgtttctgat ttaatctgat tgttttgaaa caattttagt
     3721 gaaaaaaggg tagataaaaa cgatgagaaa atttttttat atccgggtca gtagttatga
     3781 tcaatcgatc gaacgacaac tgatttccgc gaaggaatta ggaatccctg aagaatatat
     3841 atttgtagaa aaagccagcg gcaaagattt taaacgccct gaatatcaat taatgaaacg
     3901 gatgtttcga aaaggagatt tactctatct acaatcatta gatagactag gcagaaataa
     3961 acaaatgatt ttagaagaat ggcgagaatt aactaaggaa aagcagattg atatttctgt
     4021 gttagacatg cctctgttag atactgctag ttacaagcaa ctagatgggt tagagacgct
     4081 tatctctgac ttaattctgc aattactcag ctatatggcc gaagacgagc ggaaacgaat
     4141 taatagccgg caaagagaag gcgtagaagc tgcgaaacaa aagggtgtaa aattcggcag
     4201 aaagaaaatt gctttacctc ctgaatttcc tgcgatctat tctgactgga aagcaggaaa
     4261 gataactgca gtctatgcta tggatcaatt gggcatgaaa cgaaatacct tttatcgacg
     4321 ggtgaaagaa tatgaacata ccttgcacca cttttcttga tagatataac agctattaga
     4381 cgggaaggga gagtttgaaa atggacttaa cgaatcaaag ggtcgttttt actggaacgc
     4441 tgcagcaaat gacacgaacc caagccatcg gtttggttca ctctttaaac ggccattgtc
     4501 aatcaagtgt tacttcaaaa acaaatttcc tggtttgtgg tcagtattct aagtcgttgt
     4561 ttgatgattc tctctataca aaaaaggaac aaaccgcgcg ggacttgatt gcgttaggtc
     4621 atgagattac cattttatca gaagtcgaat tttatgcgtt aattagtcaa cagttgaaag
     4681 aatggcaacg gtatttataa tcgttagtaa aatttacaag gaggaaaatt tttatgaagc
     4741 gaaaatctta tgatcgttgg ggcattgaaa taactagttt taatcgaaat attgcttttc
     4801 acgtggagtg tgactgtggg gaattagcaa aaagtgttta tcaaaaaaat tacttttact
     4861 gtcctaaatg ccatcgccga tacggggtac atggaacata gaaacgaaat agtgcggtag
     4921 aaaagcgaat gaatgtagta tatttctaca aatcattcgc tttctttatt ttataaatag
     4981 ttggtcgaga gatcccgttt tcttttgcaa tctctgcaac tggaatgcct tgctttaaca
     5041 tgtccaccac ggttttaaaa actaagcgtt tttgcggatc tgttgagtta atagaatact
     5101 cgattggtcg tcccttatat cggccggctt tttgagccaa taatactcct tgccgctgac
     5161 gttcacgaat tttctcacgc tcgttttgtg caatataact tagcagtgat aaaaacatat
     5221 cgaacatagc ggtatccagg agttcatttc cagtattaaa atctaaaaac tgtgcatcta
     5281 atatttttaa cgctactttt ttctgtttca taaaagcaac tgtatttttt atatcttcat
     5341 aatctcttcc taaacgatct agtgatgtaa cgatgacgca atcaccttca cggatataat
     5401 ttagtaattt ttgaaactca ggacgctcag tatctttacc actggctttt tcttggaata
     5461 atttatcaat gttaagtcct
//
LOCUS       DAFBQH010000111         5462 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.117, whole genome shotgun sequence.
ACCESSION   DAFBQH010000111 DAFBQH010000000
VERSION     DAFBQH010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.117"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            117..1106
                     /locus_tag="LK694_002612"
     CDS             117..1106
                     /locus_tag="LK694_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK5053429.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1088..1549
                     /locus_tag="LK694_002613"
     CDS             1088..1549
                     /locus_tag="LK694_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBK5053430.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1599..2063
                     /locus_tag="LK694_002614"
     CDS             1599..2063
                     /locus_tag="LK694_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK5053431.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2077..2826
                     /locus_tag="LK694_002615"
     CDS             2077..2826
                     /locus_tag="LK694_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK5053432.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2826..3656
                     /locus_tag="LK694_002616"
     CDS             2826..3656
                     /locus_tag="LK694_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK5053433.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3656..4303
                     /locus_tag="LK694_002617"
     CDS             3656..4303
                     /locus_tag="LK694_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK5053434.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKINYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4401..5177)
                     /locus_tag="LK694_002618"
     CDS             complement(4401..5177)
                     /locus_tag="LK694_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK5053435.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5259..5402
                     /locus_tag="LK694_002619"
                     /pseudo
     CDS             5259..5402
                     /locus_tag="LK694_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1873 a          738 c          972 g         1879 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaat ttttttgaaa aatagtattg caaaataaag
       61 caatttcata gataataggt atcgtaaagt tatataactt tataatacct attagagtga
      121 gggataaaga aatgagacaa ctacacaaag aacagattga aacagcaatg gcagcgttaa
      181 aggaaaagac aattgatcat gtatatttta cagcatgtgg gggttcacaa gccgttttaa
      241 tggctggtca atatctattt gataaagaat taactgtacc ttgtcatgtt tatactgcta
      301 acgaatttaa ctatgctata ccaaaaggat tatctgaaaa ttctttagtt atcacttgtt
      361 cccattctgg cacaacacca gaaactgtag aagcaacaaa aaaagcaaga gaatttggag
      421 caatgactat ttgtttatct aatgaagaag gatctcctct atgggaagca gctgaatatc
      481 ctgttcacta tgattggggt aaagatgttg atgctagtga tttaaataaa ggaattcttt
      541 atggtttatt atttaatatt ttacttgttt tagatggctc taaaaaatgg gaaacttgct
      601 tgaatgaatt tgataagtta aatgatcttg ttattgctga aaagagcaaa tttgaaataa
      661 ctgctaaaga atggggtaaa aaccaaaaac gagataaaat tatttacaca attggtagcg
      721 gtattaatta cggagaaatt tactccacag cagcttgctg gttcatggaa atgcaatgga
      781 ttaattctaa tgctattcat tctggggaat ttttccatgg tccttttgaa atcactgatt
      841 atgatgtacc attcatctta gtgaaaagta ttggagatac aagattttta gatcaacggg
      901 tagaagactt cactaaaaaa ttcagtaata aattaatggt tttagaccaa gctgattttg
      961 acttagaatc tatagatagt gtagcgaaag aatatattgc agctgtactt agtggcgttg
     1021 ttattcgcca aatggtagaa gcgattgctt ttgaaagagg ccattcctta gatgttagac
     1081 gctatatgtg gcaaatgaaa tattaattaa taggaggttg caaaagatgg caaatcaatt
     1141 atttcacatt gtgattacaa cacattctac cctttgtgag ggttatttaa aggcaacaaa
     1201 attaatttta atgaatgacg ccacaggtat tagtgctgtt ccatttaaag aaaatactag
     1261 tccagatgat tttgaggaga gtatgaaaga aataattagt cagcatcatt tgcaacctct
     1321 aattatttta acggatttaa ttggaggaac acctaataat gtttctatta aacagttgaa
     1381 caacaataga attcaaatta ttgctgggat taatttacct cttttactgg agttattaat
     1441 ggcccagtca gctggtaaat ctatagaaga aatagatttt aaggaattac tgaataactc
     1501 tcaaaaaagt atggttcata ttaattcgct tttaaaagat gtcagttaag gtggcaattt
     1561 ctattctata aattttaaaa attgaaggag gaaatagaat gattaaatta gtgcgtgtag
     1621 accacagatt acttcatgga caagtaatat tttcttggac taaacagtat gatattaacc
     1681 atattattgt ggctgataac aaagttccag gagatccaat gtctgtgatg gctttgacaa
     1741 ttgctaaacc tgctgattgc aaattagaca ttgttaaaat tagtgatgtt aaagggatta
     1801 tggaaacatc cccaaatgat aattacatga ttttaattaa aggacctaag gaagcttttg
     1861 aattagttca aaatttacca gaaattaaag aaattaattt tggcggtgtg tctaagaaaa
     1921 gcgagtccaa acaatatgga aaggcagtct ttttaactcc tgaagaatta gaagccacaa
     1981 aaaaaattct tgatcaagaa gtgaaaatat ttgtgcaaca agtcccatct tcaagtattg
     2041 aaaatgtaga ttttagtaag taagggggga aaataaatgt tattaaaagc cattttatta
     2101 ggtttagttg gtgtggtagc tgttattgac tccaggttaa ttggtcgaca aaatattggt
     2161 agaccactaa ttttgagtac acttgccgga cttgtgctag gtgacttgca tacagggatt
     2221 gttcttgggg catctttaga attaatttct atgggattcg tctcaattgg ggcggctgga
     2281 cctcctaata tgcaacttgg tagtattatt gccactgctt ttgcaatttt aactaatagt
     2341 agtacagaaa cagccttaac aatagctatc ccagtggctg tagctggtga atttatgagt
     2401 attatcatgc gcatgataat tgctcaattt ggacatgctg cagataaagc cattgaaaat
     2461 aataaattta gaaaagctca aattattcat atttattggt catttatttt taatgctttt
     2521 gtttacttta ttccaatctt tttaacagta tattttggtg ctgacgttgt ggcagattta
     2581 gtagataaaa ttccagaaat gattacagac gccttgactg tctctggaaa tatgctttcc
     2641 gcattagggt ttgcaatgct attaagcaca atgttgagta aaaaattata tccttacttt
     2701 atttttggat tctttattgt tgcctattca ggtctttcac taattggcgt gacaattttt
     2761 gcagctctga ttgcctttgt aatggatcaa gttaaatatg gaaaaagaga agaggcgcat
     2821 ggttaatgga aaatagtaca gaaaaaaaac gtttaatcac aaaaaaagat cttcataaga
     2881 tgtgtcttcg ttcatggaca atcatgtgtt cgtggaatta tgaaagacaa atgcatatgg
     2941 gatttatgta tgggatggcg ccaattttaa ataaagttta taaaaatgat gaagagaaaa
     3001 aaagagaagc ttatactcgt catatggcat tttttaactg tacgcctcaa atgactccat
     3061 ttattatggg actttgtgct tctatggaag aacaaaatgc tcaagcagaa gaaggaaact
     3121 ttaatactga atctatctcc atgattaaaa ccagtttaat gggtcctttt gctggaattg
     3181 gtgattcatt tttccaaggt actttaagaa ttattacatt tgggattgga ttgtcttttg
     3241 ctaagcaagg tagtatttta ggaccgattt tagctgttgg cctttttgct ataccttcta
     3301 ttttagctgt tttttacggc ggtaaattag gatatgagtc tggcaataaa taccttacta
     3361 aattgtatca agaaggtctt atggatcgag taatgcaatt tgcttctatc attggtttgg
     3421 ctgtagtagg tggaatggtg gctagcatgg tgccaattgt caccccttta aaattcacta
     3481 cagggggaac agaacttgtt ttacaagact tacttgattc cattattcct ggattattac
     3541 cacttgtatg tactttggga atatataaat tgattaagaa aaatgttagt acaaatatgc
     3601 tcttattagg ttgtattttg gcagggtttg ttttaagtat aataggtatc ttataatgat
     3661 taaggctcta atttttgata tggacggagt tttaattgat agcgagatta tgtatcaaaa
     3721 gtgggtattt aattttttaa aacaagaaaa tttaagctat cctttgtatt cttatcaaaa
     3781 aaagattgga actacagcaa gtatttttga tgatatagaa aagtacaatg agggatgtaa
     3841 cagtttagaa ttgaagggaa aatttactaa atattggaaa tcgcaaaaga ttaattactt
     3901 ggatattttt cctaatatac taattgaaca gcttgaatgg ttaaaaaaga aaaaaataaa
     3961 aatggcaatt gcttcttcca gtccaatgga tactataaat gaagttgtta ccacttgtaa
     4021 aatagagtca tattttgatt gtttaattag cggaagagat ttgccagaaa gtaaacctaa
     4081 tcctactata ttccttaaag cttcagaaca gctcagaatt ccagttgaag aatgtctggt
     4141 gatagaagat tcttataatg gtataaaagc tggtaaaaga gcaaatatga aggttcttgc
     4201 aattaaagat aaacgttttt ctcaagatgt aagtctagca gatgatgtta tttacgatat
     4261 taaatatctg cgacaagaag tacaggtgaa atttggaata taaataagtt ttttgatgta
     4321 tttagagaat attagtaaaa ataaaaatca tgcccaaatc taatcaaaat tgattgcttt
     4381 gggtatgatt tcttttaata tcaatctatt actttaggat ctgaatcatt tttatgcacc
     4441 gtatctaaag tgatattttc tgtgacttca ataacataac taattctatc acaacgaact
     4501 gtggattgag aataatgaaa tggttgatta tttttatctt tgccaacctt ttctaaaata
     4561 aatagcggat ctcctactat acattgcaat aagtcggaaa gctcaggtgt tgcagataaa
     4621 ccgtttattt ccataataga gctcgtagct ttgacatcat agtcatcttc tagaagttta
     4681 tataatgaag tatattggct acctttttca aataaagttg gaaatttttc ttctgatgcg
     4741 aaaaaattat caattcccat aggggcaccg tcttcccaca ttaaacgttt aatattgtaa
     4801 actctatcag ttacattgat ttgtaattta ttagctaaaa actcatcagc ctttataatt
     4861 ttagaatgta aaacagtata tctaatttta tgtcctcggg tagaaagaga atcgctaaat
     4921 ccatgaactc cagctaattt taaactttct tttttttgag ttacaaaggt ccctttcccc
     4981 cttaatcgtt caactgttcc actttctgtt aaaagtttaa aagcttttcg aatggtaatc
     5041 ttacttactc cataaatttt acataattct tcttctgtag gtaattgctc tcctggaaga
     5101 tatttttttt cacgaatatc gttacgtata gaagcttcta cttgtagata taatggtcta
     5161 ttatcttgaa aattcatagt acactcccta aaaagttata cttcattata tcacaattta
     5221 ttaaaataaa agattaagat ataaatataa atgaactagt ctttgactaa ccaatgcccg
     5281 cttttgaaag aaaacaaatt cattctgttt taagtaaaaa tccttatgtt accactcatt
     5341 ctgaagggga tgagccttat cgctacttgg ttgtggaacc aagtgataag acaattttat
     5401 aaattcaata ttaattaaaa taaaaacaaa caaagtagat tattttactt tgtttgtttt
     5461 tt
//
LOCUS       DAFBQH010000112         5257 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.118, whole genome shotgun sequence.
ACCESSION   DAFBQH010000112 DAFBQH010000000
VERSION     DAFBQH010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.118"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            84..380
                     /locus_tag="LK694_002620"
     CDS             84..380
                     /locus_tag="LK694_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HBK5053436.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(1024..1977)
                     /locus_tag="LK694_002621"
     CDS             complement(1024..1977)
                     /locus_tag="LK694_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK5053437.1"
                     /translation="MKKLVVGILILASFGLAACGNSDNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(2000..2755)
                     /locus_tag="LK694_002622"
     CDS             complement(2000..2755)
                     /locus_tag="LK694_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5053438.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIIAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2752..3711)
                     /locus_tag="LK694_002623"
     CDS             complement(2752..3711)
                     /locus_tag="LK694_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBK5053439.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3704..4654)
                     /locus_tag="LK694_002624"
     CDS             complement(3704..4654)
                     /locus_tag="LK694_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBK5053440.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4804..5256
                     /locus_tag="LK694_002625"
     CDS             4804..5256
                     /locus_tag="LK694_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBK5053441.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPVEKEKPKKKKSFWDRLF"
BASE COUNT         1741 a         1065 c          907 g         1544 t
ORIGIN      
        1 aattactttt tgttcacgga ttattaacaa ctcttcggta gagtaaactt atcaaataaa
       61 aagacttctt tggaggaatc actatgaagg aatatataaa aaacatctat tttatcgaag
      121 aaacacaaaa tatcgagggt tcttatattg aagtaaaaac actctttgtc aatgaagata
      181 aaaccaaagc attggatatt tataaaaaat tagcttcaaa aaaaacgaat agttttggat
      241 tgatattaag cgagtataaa atcaaagcag aagagagtta tttctatcaa ttattaaaac
      301 ggtggagtaa gcttcccgcc gatttttatc gaaaaatgca gatcatcaac tatcaaccgc
      361 ttgctgagac acatgcctaa atagaggttg tgaaatcagt gctttgagct aataccgaag
      421 gtcagctgat ggaacatcgt ttagaagaga ttgtgacaac tacgatttgc tttaagtaat
      481 aagaacgctg gacagaaaat agcttctcat attttctagt ctagtggact tattgccgag
      541 aagcagcaga tggaacatcg tttagaagag attgtgacaa ctgcgatttg ctttgagtaa
      601 taagaacgct ggacagaaaa tagcttctca tattttctag tctagtggac ttattgccga
      661 gaagcagcag atggaacatc gtttaaagaa gagatcggta caaaataaaa atatctaaat
      721 ttaagtttta tctaagtttc gctttgtacc tttgattcaa atcaaaggag gcgatcttga
      781 tgctgaaaaa atggatcaca ctattttttg ccactcccca ttctagaatc aacccgtatg
      841 atgtagagaa tgaattcacg ttgaaaaaag atttcccaaa taaataaaaa ccatccgtaa
      901 aatcatcaat acgattttac ggatggtttt tgataacttc tttgaggata ggctcttgct
      961 atttaagaat gtaagaaaca ttattgttac atagtatgag agtttaatcc tctgttattt
     1021 atgctattta aatgcttgat tcacatcttc gatcatcagt ttcattgatt ctaaaccgcc
     1081 cccgcttaaa taccagacat ctggttcaag ggagatgatc tgctgatttt taccagcatt
     1141 cgtttgtgct accagttcat tggcagaaat atcatctttg ctgtcgtctc caccaatagc
     1201 ttttgttctg tctactacaa ataagatatc cggatttttt tccagcacat attcataaga
     1261 aacactttga ccgtgagtgg atgcttcgat ttgatcatct gcttgttcaa agccaaataa
     1321 atcatggatg aaaccgaatc tagaaccaga tccgtaggct gataattgcc cttcattcac
     1381 aagtgtgact agtgcttttt tgtcagtagc ttctgctttc tttttcgtct gatcgattgc
     1441 actagataat tcctctaatt gagttttagc ttcttcttgc ttaccaaaga tctcccctaa
     1501 agcagtgaca ttttgctgga aagattccca tggatttttc gcatctaaag aaaggaaaat
     1561 ggtcggagca atttgactta gatctttttg aaaatctctt tgccggccag aaatgatgat
     1621 caggtctggc tgaagctgat tgattttttc taagtctggt tctttgatcc cacctgctga
     1681 ttccactttt ttataggatg aaagatactc aggaaggctg cttgtagctg ctccaactac
     1741 cttatctcca acaccaagag catccatcgt atctaaagaa ccattgtcga atacgacgac
     1801 tttttcagga ttctttggca cctcgatttg atcgccatta ctgtctttta tcgttaatgc
     1861 cgtttcagct tgcgtactgc ttttctttgt atctgcctga ctggtgttgt cactatttcc
     1921 acatgctgct aaaccaaatg atgctagaat caagatacct actactaatt ttttcatctc
     1981 ttctctccta atgttagttt tagaaataaa gacagaatct gcgaccttca atctcacaaa
     2041 ttcgtatgtc catttcgtac aattcgtcca gtacatcttt ttttatcatt tcttgggtcg
     2101 ttccttgcgt gaataactgc ccatctttca ttgcaataat ttcgtctgca taacttgcag
     2161 caaagttgat atcatgaaga acaataacaa ttgttttccc gatttcttgt actaattttt
     2221 ttaagatctg catgatttga accgcaaaat tcatatctag attgttcaaa ggttcatcca
     2281 gtaaaatata atccgtgtct tgcgctagca ccattgcaat atacgcacgt tggagttgac
     2341 cgcctgaaag agtgtcaatc gattgatccg ctatttcttc taacccaagg tgttccagtg
     2401 attcctgaac gatttcctcg tcttttgtcg tcaattttcc tttcgtataa ggaaatcgtc
     2461 caaattctac taattctttc acggtgattt ggacatttat ttcattcgat tgtcgtagga
     2521 tcgataattt tttggctaat tcattggatt tccaagcctt tacttcgtta tggtctaaat
     2581 agatttcacc tgtatctttt ggtatcaaac gactgatcat cgacaacacc gttgactttc
     2641 cagcaccatt tggaccgata aaagcagtca acttcttttc aggaatcgtt aactgtacat
     2701 ctttaaccac tgattttcca tggtacagtt tattgacctt ttgtaaatca atcataggat
     2761 actcttcctt tcgcgaatca attttgtgac aaaataaatt cctccgacaa attcaacgac
     2821 gacactcact gttgtattcc agccgaaaat ctgctctact gcgagctgtc ctcctgtcag
     2881 aaacagcata gctaccagaa agcttccgat aaatagctga ccatgacgat aagtagaaaa
     2941 gacacgataa ctgatatttg cgacaataaa ccctaaaaaa gtaactggac ccacaagagc
     3001 cgttgatgtc cctgtcaaaa gactgacaca acataaaatc aaaaattcaa aacgatctgt
     3061 gtctatcccg aggtttactg cttgttcatt tccgagatgg aaaacgtcta gcacatgtcg
     3121 atatcgaaat aacaggtacc agcagccgat aatgatcaaa gaagcaggca aaagatactg
     3181 actctggata ttgccaaagc ttgcaaataa tctgccttga agcaaatcat attcattcgg
     3241 atccatgatg acttgtaaaa aggtactgac actaccaaaa agcgtaccta aaatcatccc
     3301 tgacatcagt aataaaaaaa gattcgctct catttttttc aaaagaaaga aaaataacaa
     3361 tagactcagc ccgaccatca gcccaacgtt caataagaaa aaaactgtac ctgtctgctg
     3421 aagcatcgtc actcctccga taaagaagaa aaggaccgtt tggacaagga catataacga
     3481 ctcaagtccc aatactgaag gagtcaaaaa ctggttatgc gtaattgtct gaaagctgat
     3541 tgtggcactg ctgacagcta gcgagacaag gataaatgca aaaattttct tactcctcaa
     3601 tgcccatgca aaatgccagt ttccatatgt atggatcgtc aagtacattc ctgtaacaaa
     3661 taaaacaaga acaagcatac caataaaaat agatggcttt tttttaagca tatcgtcgcc
     3721 cccttatcag aagataaagg aagatagccc caccgatgat tccaacgatc acactgactg
     3781 gtatttcata aggaaaaatc aacacgcgag cgacaagatc gcaaaatact aagaacaaac
     3841 tacctgctaa agcagttacg ccaattgttt tgtgcacttg atctccatat attctagaga
     3901 caagattcgg aatgatcact ccgataaaag gaatgcttcc taccatgatc aacatgactg
     3961 cactagccaa tgatactaag ccaatgccta aaaactgcat cttctggtaa tctattccca
     4021 aatttactgc aaacgattcg cccagaccaa tgatcgtaaa tcggtaagca aatagccaaa
     4081 ccaagagcaa caatggaaca acagcataca ataactcata atttcctcgt atcacagtcg
     4141 caaaatttcc ttgaagccat gaagtcatat tttgtaccaa ctggaattga taagcaaaaa
     4201 aagtagcaac tgaaccaatg atattcccta acatgactcc aatcaacgga agcatcaatt
     4261 gatttttctg ggggaacaat cgcgtaaggg ataaaaaaag caatgttcct gcaaaagcaa
     4321 agcaaaatgc tgtcaaggat cggacaatca aggatgcacc aggtaggaaa atcatagcca
     4381 ctaaaatacc taaacgcgca ctatccatcg tgcccgcagt cgttggtgaa acaaatttat
     4441 tttgggtcaa atgttgcatg atcaacccac aaatactact ggtcgctccc acaaggatca
     4501 aacaaatcgt tcgtggcaaa cgagtcacta gcaagatttg taagctttgc ccatctaact
     4561 ggagcagttc tgacaattgt acattcgata ctccgatgaa caatgaaata ctacttaaaa
     4621 taactaataa tgctactagt cgtccgtagc gcatccaatc cacctttcta attgagaatg
     4681 attttcattc ttattttaag tgaatcgttg aacaaaagca accgcttttt catatgtttt
     4741 aggtattatt gggaatatgc tataatcgta agaaagacaa agagaagaag gagggtattt
     4801 tttatggcac aagatgatgt aaaaagccat ttggataagg gactttatgg tacaccttta
     4861 gtgaaccctg aagaacagca taaatattta gggacattca gggaacgctg ctatcttagt
     4921 atgactgttg ctgaaatgat ccaaccaaaa aacaaagaaa actttttaaa agaactcaaa
     4981 aaacatccag atgcttccat actcttgaat ggggctctac ccctttctgt ccaaaaccaa
     5041 tatatccagc tagcaacgaa aaacaatagc cgatttactg tagtcaacga ttacgtcact
     5101 gcaaatcctg aggaattcgg attgctctta acagcaaaag aagcagtaaa tgaacctgtg
     5161 attgatatcg aaaaaaaata cccagaagaa aactcttcgc cagttgaaaa agaaaaacca
     5221 aaaaagaaaa aaagcttctg ggatcgttta ttttaaa
//
LOCUS       DAFBQH010000113         5212 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.119, whole genome shotgun sequence.
ACCESSION   DAFBQH010000113 DAFBQH010000000
VERSION     DAFBQH010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.119"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            604..954
                     /locus_tag="LK694_002626"
                     /pseudo
     CDS             604..954
                     /locus_tag="LK694_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
     gene            948..1370
                     /locus_tag="LK694_002627"
     CDS             948..1370
                     /locus_tag="LK694_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053442.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTNLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2540..3508
                     /locus_tag="LK694_002628"
     CDS             2540..3508
                     /locus_tag="LK694_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK5053443.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3525..4049
                     /locus_tag="LK694_002629"
     CDS             3525..4049
                     /locus_tag="LK694_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBK5053444.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTETHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKTEKKWWQFWQ"
     gene            4425..4706
                     /locus_tag="LK694_002630"
     CDS             4425..4706
                     /locus_tag="LK694_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053445.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            4708..5124
                     /locus_tag="LK694_002631"
     CDS             4708..5124
                     /locus_tag="LK694_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053446.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1972 a          894 c          867 g         1479 t
ORIGIN      
        1 aaaaaaaaga aaaatccgct tatgcaactt cttctttttg tattttgggg cgctacccca
       61 atccccaagt atcggttatt taatagcgcg gaaatccaaa gaataggacg taccctgaaa
      121 caaaaaaaga ctgctctcac gagcagccca aaatgagatt atcctattca tcaacataaa
      181 aatattacaa agtgaagaac tagagaaaga caaaaaaagc atatcaaatc ctctcaaaaa
      241 taacaagtct ttaaaaaatg acagataaaa atgcacaaag aactaaaaaa gagaaatcgc
      301 gtaaaatcaa gcattagaag ctagtaacta gctagaccaa ttaaaaatca caaagtgagc
      361 gtctctatcc attggtagag gggctacttg gcatagtgta cattatgtta actagctcga
      421 taaataacga ttatgttccc aaaacaatat tttatgaact ataacaaaaa tttgttatcg
      481 catattgtat attttaatta tataattata ggtgtatacc acttaaattt aacacaaagg
      541 cgtggttatt ttgaaaccca aaaagaaaat ttatctaggt ctcctctttt ccttgatgat
      601 tggaatagtc aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat
      661 tactgtggtg tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca
      721 caactaagaa aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac
      781 agccgtcatg cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat
      841 gtgtggaatc cttggtataa ctacccaact gtaataagtg caaatgcagg accagttaca
      901 attcctgtac ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac
      961 ccattgcttt agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac
     1021 aagaaataca aaagactaat ctgattcctt ttctaggaac ttggcaaact aaagatcatt
     1081 cagaacgtct agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct
     1141 tagtaaaaga gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac
     1201 aagttttcat ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat
     1261 tagattacac ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg
     1321 atgggacaga agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg
     1381 tttgatatta caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt
     1441 ggctagcatt tgctagcctt ttttgtattg tggagcgtgg tttttgaggg ggattcaagg
     1501 gggagcttgc gcccctttga caagcggtta tttgcaccaa atacgtaggt atttggctag
     1561 caaatataga gcttgccaaa cactttagtg agttttgtag aaagtacctt taaaggtact
     1621 ttcacattac gaacgatacg tccgtaggac gtactgaaag ggtgggattt atgagcgaac
     1681 taatgcaaaa tttggctagc aaaaaaagtt aaaaaatata tgctaataat cgagcgaaat
     1741 gaacactttt tcaagtgagc gaatccgcca tgacaaagct gaagcagttt atttcagctt
     1801 tgtcatggcg tgtgcctaga cttttaaaaa agtcggcaca cgagcaaata tctttatcaa
     1861 gctctaattt gcgttttaag acgtttgaaa cgtctaagta tataaatata taagagtagg
     1921 tttaagagtg cttaaatcga attttagagg gcattcagac aactttaggg ttgttagggc
     1981 ttgccctgac cgcctgtaag gcgctttaag aacaagataa gggatttata ctaagactgt
     2041 cttaaaacgt ctcaaacgaa ggctcatagc ttctcaaaga gttccttatg aacattttta
     2101 gcaaaggagc cggaagcgac tgcgcagtat aatgtgagag cagagcgatc acaccttttt
     2161 aagcgactgt agggagtaag attcccagtt gagaaaaatg cgcgaatctg tttggagaaa
     2221 aaagaagtgg ttttttaggc gggaaatttt gtccccctct tttataatct ctttttaaac
     2281 ctctttttaa acctctttac cgtcggctat atccttactc tcccaaggga atgacgcttt
     2341 tatctacatc gttttgtctg tttatctaca tcgttttgtc tgtttatcta catcgttttg
     2401 tctgtttatc tacatcgttt tgtctgtttg tacattgaat tagtacatcg acgatgttaa
     2461 tataagatgt agataaacag gcattttgta tttcataacg tgtatctctt gtgcgagtaa
     2521 gaaaggagct attttaaata tgtctaatga aatcgtaaag tatcatcatg aattaaatac
     2581 aattccgttg cgaaaattta caccagtcga aatgaatttg ttcttttcta tcgtatcaag
     2641 aatgcgtgat gtaggtgata aaaaagtaca atttacattc gagcaattaa aagatttaag
     2701 caattataaa gcaactgcaa atgttcgatt catagatgat ttagaaacaa cgtatgacaa
     2761 attaatggat ttacgttttg gtagaagaag tgctgacggt ttgcaacgtg aacgttttgt
     2821 tttatttaat caatttaaaa tcgacggaaa atctgatatt ccttttgctg aaatacaagt
     2881 tcatgaaaaa gccttaccct tacttaataa tcttgaagaa tgggtgcgct attctctcca
     2941 acaattcaat gaattagaaa gcagttattc aaaaacaatg tttcgattac taaaacaata
     3001 cagaaccaaa ggtttcgctt atttttcaaa agaagacttt catgaattac ttgatattcc
     3061 taaaagttac aaccaaggta cgattgatca acgtgtgttc aatccaatac gacaagaact
     3121 aaccgcaatc tttaaaggtt taacaatcaa aaagaaatac ggaaaaggac ggggaaagcc
     3181 tgtcattggc tatcaattta catttaaacc tgagataaaa aaatcagacg actttaacaa
     3241 acatgcccca acaaaagaag aacaaaacaa agcattagaa ctagctaacc aacaagttca
     3301 atctgaaaaa gcacctactt cattacaaga aaaaatcaaa gaacaaaagc gaaaattgga
     3361 acttttacaa cagttagaaa agttagaaag agaacaaaat acccaaaata aagaaaataa
     3421 tgcacaaaaa tcagagataa attccgatat tccttcagaa ctaaaaaaag acttattaga
     3481 caatcttcaa aacttattca aagattgagg ggtttttaga ttttatgaca gaaaaaacaa
     3541 tcaaagaact tgctgaagaa ttaggagtta gtaaaacagc aattaacaaa aaagtttctg
     3601 acgaaaatcg aaaactttgg ttttcgaaaa tcggaaacaa gtttgtgatt aatgaaaccg
     3661 gacaaaaggc tataaaatca atgtttttta acgaaaacga aaactcaaat caacaactag
     3721 ttggcgaaaa aacgaaaacc gaaacccaca aaaacgccaa ccaagttccc gaattagaag
     3781 tcctttctct cctagaacaa cagttaaatc aaaaagatat gcaactacaa gaaaaagacg
     3841 agcaaataaa acaaatgcaa aaattactcg accaccaaca acaactcacc ctgcaagcca
     3901 accaacaaat cgagaaactg caggaacaac tacagttgac ttatacggaa gaatcttcgg
     3961 aaaataaatc aacgactctt tctgaagaaa cagaatcgat agaaaaacaa tcaaagacgg
     4021 agaaaaaatg gtggcaattc tggcaataaa accagcaatt taaattaata aaaattgctg
     4081 gttttattct tctttttgct tttgcgagca gagcaacatg gacccatgtt gcgatatttt
     4141 ggtggcgccc caatccccgt tttttcgttg aatcttactc ggactgcact cgttttacag
     4201 ctgatttttc tgaattttga aagagcataa ggaaacgtgg tatcactttc ttacgaaagg
     4261 tgctagatag gctgtgccta tcacgtttct cagaaaaaaa tttgctatca cttttttgcc
     4321 attgtggcta ggctatgtat agtgttttgg cgaaaaatag ctatacaaat actatatttg
     4381 tatggtatac ataaattata cgctgatacg aaaggaaaat ttccatggac attttagttc
     4441 gaaatatcaa tcccaaaatt attgcaagaa tagataaacg ttgtcaggaa ctcacaacca
     4501 aaacgggcaa aaaatggagt cgaaataaat atctaaaagt tctaatcgaa agtgattttg
     4561 accgtcaact tatgaattac aaaaaagatc aattcgattt attactcgaa aaatttattg
     4621 ccattcaaac agaaaatacg aacatactga atgactatgt tcacacaaac aaccaactca
     4681 ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa acacgtaacc
     4741 ctttatccca gtcacgttga cgttttagat tccatcatcg aaaaaaatcc agacgtcaac
     4801 aattattcag aagccattcg ttttttatgt atgagttatg aacctgatac caatacaacc
     4861 acaaccagta accaagcaaa actcaatgcc atgggaaaag aaatttctat catgacagaa
     4921 atcatggcag aatttgctga tttatatcta aaagatactg gtgttcttat cggtacaaat
     4981 tctaaagtgt atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc ttcccgacga
     5041 aatcaaccca aaaatcgtca aaaagaaacc aatcaaacct ttacttctgg aaattttgac
     5101 ccaacaaact ttctaaaaag ataaaaaaag aaaccgatcc ctaaaaaaat tcctcgattt
     5161 ctttttaggg atcagtcttc cagcaagatt tgcaagttgg taccaacttg cg
//
LOCUS       DAFBQH010000114         5120 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.120, whole genome shotgun sequence.
ACCESSION   DAFBQH010000114 DAFBQH010000000
VERSION     DAFBQH010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.120"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(207..1169)
                     /locus_tag="LK694_002632"
     CDS             complement(207..1169)
                     /locus_tag="LK694_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK5053447.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="LK694_002633"
     CDS             complement(1171..2610)
                     /locus_tag="LK694_002633"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBK5053448.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="LK694_002634"
     CDS             2795..4741
                     /locus_tag="LK694_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HBK5053449.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..>5120
                     /locus_tag="LK694_002635"
     CDS             5005..>5120
                     /locus_tag="LK694_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK5053450.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEET"
BASE COUNT         1665 a          855 c          841 g         1759 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat
//
LOCUS       DAFBQH010000115         5030 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.121, whole genome shotgun sequence.
ACCESSION   DAFBQH010000115 DAFBQH010000000
VERSION     DAFBQH010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.121"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(45..1364)
                     /locus_tag="LK694_002636"
     CDS             complement(45..1364)
                     /locus_tag="LK694_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK5053451.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="LK694_002637"
     CDS             complement(1361..2014)
                     /locus_tag="LK694_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5053452.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2822
                     /locus_tag="LK694_002638"
                     /pseudo
     CDS             2302..2822
                     /locus_tag="LK694_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2724..3440)
                     /locus_tag="LK694_002639"
     CDS             complement(2724..3440)
                     /locus_tag="LK694_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBK5053453.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3564..3749)
                     /locus_tag="LK694_002640"
     CDS             complement(3564..3749)
                     /locus_tag="LK694_002640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053454.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3976..5030
                     /locus_tag="LK694_002641"
                     /pseudo
     CDS             3976..5030
                     /locus_tag="LK694_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT         1614 a          970 c          694 g         1752 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     2761 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     2821 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     2881 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     2941 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     3001 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     3061 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     3121 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     3181 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     3241 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     3301 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     3361 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     3421 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     3481 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     3541 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     3601 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     3661 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     3721 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     3781 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     3841 gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt
     3901 gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc
     3961 aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat
     4021 ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt
     4081 gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa
     4141 catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg
     4201 aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc
     4261 tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat
     4321 tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc
     4381 ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg
     4441 aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa
     4501 gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt
     4561 ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt
     4621 ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag
     4681 tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc
     4741 agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa
     4801 gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg
     4861 cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt
     4921 gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat
     4981 caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca
//
LOCUS       DAFBQH010000116         4809 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.123, whole genome shotgun sequence.
ACCESSION   DAFBQH010000116 DAFBQH010000000
VERSION     DAFBQH010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.123"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(91..1176)
                     /gene="xerS"
                     /locus_tag="LK694_002642"
     CDS             complement(91..1176)
                     /gene="xerS"
                     /locus_tag="LK694_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HBK5053455.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3252..3770)
                     /locus_tag="LK694_002643"
     CDS             complement(3252..3770)
                     /locus_tag="LK694_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HBK5053456.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1580 a          786 c          730 g         1713 t
ORIGIN      
        1 tataaaaatg ataatgaagc ttgcctttaa agttgggctt ttatctaatt ttagagacag
       61 gcttcattat tatactattt tttactacat ttacaaagcg ttcaaagcat caattgtagc
      121 tgcattgtct atatgagtat ataaagttgt tgtatttgta cttgtatgtc ccaattgttg
      181 ggcagttaat accaatgaat ttgtctgttg atagagtcta gtagccaaag tatgacgtaa
      241 tttatgtgga ctgacttgga ctctgaatcc ttttgaatat tttgctacca tccgttcgat
      301 tgcaataccg gatatacgct tggcttctcc ttgataaatt gctagaaata aagctttttc
      361 gttttctgtt actttgtatc gttctttccg aatagctaga taattagcta aatattccat
      421 tgcaaatgct gctatattta ctgcgtctct cttatttcct tttctagtta ctaccacatt
      481 attcttttct aaagataggt cctctaaatt aatatttaca acctcggaga gtcttaatcc
      541 agatcctaaa atcaatgcat tgatcgccag gtctctttct acatctcgtt gataatagat
      601 caaagctcgt ttgctcaaag tttttttata ttcattggca atataatcta gataattttg
      661 tgtatcttcg tctagaaata attttttctc gattgcgttt gcacgggcag ctaacgtagt
      721 tttatctgtg atagttgcta cttgatccat aggattattt tctaagtatg gttttcctaa
      781 aggattattg gaacttttcg aaagatattt aaatagaacc tttaatgctg tgatatttct
      841 attgatagta gaagtagata gacctaagtt attattatct ttgagcttag tttttgttgt
      901 ctcttttttt acattttcct ttgtgcgacg tttcaaatga gatttgtaca agtttagatc
      961 ataggcagtt aactgtctta tcgaggtgat tgaagtatcg ggaacgatag actcactcag
     1021 aagccattca taaaagagct tataattttt tagatattct aaagtagttc gaggtgattg
     1081 atctttatcc tcacgatctt gaacaaaatc ttttatataa tcgggcatat gaagtatgat
     1141 tgtatctatt ttttgatata tttctgtatt tttcatgtta ttcatccttt cgataagtag
     1201 gggagaggag tatctctata attggactat accaattata gagtgttttg tcaattggta
     1261 tactctgaaa tttattaatt tatttcaaaa aagcagataa ataggtatag atttaataat
     1321 ttaaaataaa tggtatacat gaataggaaa ggaaacagaa ggaggcaatc ttaatttttt
     1381 aaattgtcta aattatatag aaagaacaat ctttctatat aattagacat aagagagact
     1441 acggttgatt tgatattaac aagcgtatat gaatgtatat acaaataaaa aaggggaggg
     1501 agaagttaaa gaaaattcag agaagtgggg cactcagtag tattaacaat ttaaaaggaa
     1561 tttcttaaag aaaatgggtt gcatcctgga gatacaattt ttttagataa agaaaaaatt
     1621 caagcaacaa ttcaaaaaaa tcttaactgc tgagattgat gtagcaatat ctcaatcatc
     1681 aacaaagtac gatcctgcct ttcaaaagct tattgataaa tgtttattca ttataaaaat
     1741 aaaagaaaaa tttatgtcag cttttttcat caaagtggta atgataattc gtctattaat
     1801 tgtatttttt aggaaatatg gtataattgt tttaacgaaa aggtaattca tgatacacca
     1861 aaaaccacta gctggcgggc tagtggtttt cttttacgtt ctattcagat taacgggtag
     1921 gcttactgtc ttttttggca ataattttgc aatcaagcca tttacagatg taatgcgcgg
     1981 atacttctgc taagattgca aaaaacatat cgaaaaggta attcataata caacctcctc
     2041 cccttgccat aataggccgg ggtagcagac agcataaata ttataccata actgaaaatt
     2101 tcattttagt acatttcaat atattgatga accagcacaa taaaacatgc catgactaac
     2161 agaaaaaaac ttattaaacc agatataact tgtaaacatc caactatata gaaaacgtaa
     2221 attttctata tagttggatt aagataacga ctatttattt tatcatattt ttactatacc
     2281 ctatactttt acttggtata tttatgaata atcgcaaatt tacccctata ctactagttt
     2341 gtaagttaga ttaataaaaa tactaaagtt atttgttctt ttattaaata cacctacaac
     2401 ttataaattg atggtattta gataacacga aagaataaaa ataacagctg atagtaaagt
     2461 ttgttgacga ttgctactca atcgaaagtg agttagtgcg tatgaacttg cttctgaaaa
     2521 cttatgaaat gaaagacctt ttacatcttg cagaaatcgg tactgggatt aaactaagca
     2581 ataaaaacta atgttcccct cattctttga acttctacta tttgatttat ttattctgat
     2641 aaatgacgat ttcaccttca ggatcttcaa atcccgtata gaatttattt aagatctctt
     2701 cagtatcagt aataggatag gcgataggcc acataccctt tgatggttag aataaactgc
     2761 tcaaaatctg tatcgtatgt tggctttagc atattaacaa aggctatctc caaattataa
     2821 accataagcg ttgaatcgtt cttaacaatt tcaattcttc ctaacccaaa gtcagaagat
     2881 ataattaccg gacttgcctt acccttcatg tgggttgttg agattccata tttaaaggga
     2941 attgtcggta ctatcggcgg tctgaaaaac atcccaaaaa taaaaaaatc tttcccctgc
     3001 tagtctccta agtccctttg tagcggatat ggcaccataa tctcatttta tcgaagtgag
     3061 tcatggttta tctgttggcc aaccaaaatt gatacaatct aacgatgcac cctctacggc
     3121 attctagggg agtgcacttg cattcaccca ataaaaaccg gcagttagca aagaacttat
     3181 gtagtcgtat gttcttactg ttaacataca agaggtctcc tttccacaat tgcttagcag
     3241 taaacgggaa tttacaaagc taatctcttc cattttctta tctttgttct aaaagtccca
     3301 aatggagcaa ctgtatttac atgaataaat ttatatactt cccatacagc cgttttggtt
     3361 gcctcgtctg cccattttct tttgtgtgca gtgaatagat cttcatcact cattgaatca
     3421 atcatcatgt taatatcatc aatattacga ttgagaattt cctctaattc tttcaatggt
     3481 agatgtgcat aggtatttgt aaaccactta tataactctc ctaactgatt ccatttaaat
     3541 tgatcagaag gcgttttaac agataatcca tttctttcat cattttccca ttttagaatt
     3601 aaattcgtcc aaccaacttg atatgctaaa ttttcagcag gtgttctatc cacttcctca
     3661 actcttttat ccttcaattc ttcaggaata gtttcaaatt ctaaaatata tttagtataa
     3721 gtcctattta tttctttttt taattcttct ttatttttat atgttctcat tatattacct
     3781 ccataaattc ctaattgtct cctttaattc atctataatt tcatccacga tattttaatc
     3841 cgttctacaa tgcatatctg atatgcattg actgtctaac tcatatgtga atgatttata
     3901 aaatatccca acgtctcatc taaagaaatg ttgatcgtca gcaaggagct aaaaaatacg
     3961 aatatatttc ttttgtagta aaatcggact ggtttgcata tcattttctt cgtaaatatc
     4021 aaaatcatgt taactactat caattactaa taaaaactac cactttaagc gttaagaact
     4081 gttaagttta gttaagtgag gatagttcac aaaatccatt gttaaacttc acgaaagtgg
     4141 taaaactcaa aatgctttat ataggattac agcatcgctc tttcttcgat tgcgcgttgg
     4201 gtaaaaaata ttatgaagta aaaattgacg atgacacgtt ccttacggct catcagatga
     4261 aacagctaca aaaacgaaat gatcaactcg aagaaaattt aattctaaaa aagcgattgc
     4321 catattcacg cctcgctcga cgaactatta aaggtcaaga tttacctgaa gatcatcctt
     4381 tttaggaaat tgaagctttt tatgttgcac aatgcattta taatactaca atgatgtttt
     4441 caccagatat tcttctaggg tgcggtgtaa tgaaacagat acacttgcaa aagaaaattc
     4501 accgcgaatt tactaaaatt atgaataatt atgttaatct tccgaaattt ttttccaaaa
     4561 ttaagggatc aagctggtgt tatagattgt ttaaggttgg caaaaaaata acttcttttt
     4621 aaacaattat agaaaaccga tactctgtta agttggttga cacggtgtcg atttttatga
     4681 ctttcttatt ctgaaacttc actaacggac agtacctaaa aagtctatag tatggacgtt
     4741 tacgagtact attttatcaa aaatgctgct catttcaact tatatgtttt agtgaactac
     4801 ccccactta
//
LOCUS       DAFBQH010000117         4791 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.124, whole genome shotgun sequence.
ACCESSION   DAFBQH010000117 DAFBQH010000000
VERSION     DAFBQH010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.124"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(75..1073)
                     /locus_tag="LK694_002644"
     CDS             complement(75..1073)
                     /locus_tag="LK694_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBK5053457.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1148..1444)
                     /locus_tag="LK694_002645"
     CDS             complement(1148..1444)
                     /locus_tag="LK694_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBK5053458.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1437..1730)
                     /locus_tag="LK694_002646"
     CDS             complement(1437..1730)
                     /locus_tag="LK694_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053459.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1876..2427)
                     /locus_tag="LK694_002647"
     CDS             complement(1876..2427)
                     /locus_tag="LK694_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HBK5053460.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2601..2900)
                     /locus_tag="LK694_002648"
     CDS             complement(2601..2900)
                     /locus_tag="LK694_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HBK5053461.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2927..3193)
                     /locus_tag="LK694_002649"
     CDS             complement(2927..3193)
                     /locus_tag="LK694_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBK5053462.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            3540..4223
                     /locus_tag="LK694_002650"
     CDS             3540..4223
                     /locus_tag="LK694_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053463.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
     gene            complement(4260..4757)
                     /locus_tag="LK694_002651"
     CDS             complement(4260..4757)
                     /locus_tag="LK694_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK5053464.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT         1428 a          912 c          696 g         1755 t
ORIGIN      
        1 taaaaaaact gattctaaga aaattaaata atttttctca aaatcagttt ttctttgata
       61 aataatattt tcaattattt aatcatttga aaattgttag tcattccaat acgttgagaa
      121 attcttccat ctgtataaac acgaaattgt tttccaccag ccatggtcgt gtttccatca
      181 tcatccgtta caatattaat tgaatagtag cttccatttt catcttgcat tagatctttc
      241 atatattcat ttgtgctaat taaattagta tttgatacat ttttatcagg agaatcatac
      301 aaccataaag ctttattatt tagcacagat tctttagcag cttcccaact tgaaattcct
      361 gaactacttg tagattgtgt cgttgtagtt gttgcttgtt gagtagattc ttctactact
      421 ggtgtttgag gagtttgtcc attcacaata tcactgaaac cttgttttaa ctgttgattt
      481 tgagtttcgt taaagtacag ataattattt tcattacctt gattttgttg cgttaaataa
      541 actttaggtt gtccattttg aaaaccgaaa aagtaaacat gctgccctaa atattctcca
      601 gtttctacat cagaataaac tgctactaaa ttgaaatctg catgaccagt tccatcttct
      661 gaccattgta cggtaactgg tgcttcattg attgccattt tccaatcgcc attaatcacg
      721 gcttgtggta aaggagtgcc atacagactt acattattct gaagattgta gcttttatat
      781 tcttgaccta atgtctttcc ccattgtgtg acaaaagttt ctaatttgga agctttattc
      841 gtatcccaca aagtattctt aacctcttca gaagaagatt cagtgattga acttgcactt
      901 gtttccacag attgcttcat ttctgttgta gtttgttcag ttgattgcgt tttacttttt
      961 ttagtctcaa ttgttgtatc agtttctata gtcgctttag tcctcgtatt ctcagatgta
     1021 caaccagtga ataaaattcc cattcctatt agtaataaca gccatttttt cattatattt
     1081 ccccctttca atttctccct cacgtactta ttctatcaga caattaattg atctaaatgg
     1141 taatctttta atcgattctt tcccaataat gtcgatagcc cgtatggcag cggtctcttc
     1201 cttgaggact ctcagatgga ttaaaactag tagaaatttc ccttccacat cctctacagc
     1261 gataagttct cattattttt gggctattag tattattttt cttagttaga gctatgagtc
     1321 caactatgat taccccaact gtcttaatta cttttccttt atctatattc ggatgcgcaa
     1381 gcatatattt atataaccaa tatccaccgt aaattaaacc acctaataca atcagcttag
     1441 ccatgtactc gtctcctttc tttttgttta attacttttt gaatccatct tgcagagaaa
     1501 ccataaactt ctgtcaattg acggatatct acagtttttt cctgttctat catgttttcg
     1561 attctttttc taactaactc acgatcgtac attttcattg gaaaagaaat ctgttgccct
     1621 cgaaaagcaa gataaaattg atagagcaat tctgaatcca gttccatttc ctcaaaaaag
     1681 tcattcatac ttttataaga gttactcaaa caagtttcat caatctccat ctacacaccc
     1741 cctgttattt tttacataat gaaaatagca tattactggg tacattgata attcatataa
     1801 catgtatcat cacatgtatt tttaaagtca aaaagcatgt aaaactactt ataagtaaca
     1861 tgagaaaata aaatgttatt tttcttgaaa taataattga ctatctgcca attgccattc
     1921 attacgaaca tgaatactta gtgggtggga taacttacct tctgcattta tcaatacttt
     1981 ggtcatcttc ttttgtttcg cactcaattg atcgtaaata gtctttgctt gttcataagc
     2041 cacttgactc gtcgctgtaa tcgaaccaca agcaaaaata atggtattga ttttcttttc
     2101 tgtaaaaaca gtcgttaata tttccaaaga ttgttcatca tttcctcgaa gaaatacccg
     2161 aggacttttc gcttttgtgt acgaaaaaag attcacagca ataaaaccag taaatcctaa
     2221 ttgtcggata ttcttttcga ttaataacat actcaaatca ttcgtaaatg gttctgtatc
     2281 tgttggacga atagtaatga ctactgctaa tttatcttcc gcttcttttc ctttgatcgc
     2341 ccattgtcgt ttgaaaacat agcgatgggt atgatgagga tctcgccaaa cttctgtttg
     2401 tgtggtcact aattcttttt caatcatctc tttttctcct atgctatttt ttgttcaagc
     2461 acttcaatta ccattgccgt tataagtgtc aatactagtt gaaaatattt catattcttt
     2521 ctcctttttt cttcagaatt tgatgaatcg cggtaaggat atcttctaaa catttcgcaa
     2581 tcattctcaa ataatcgatc ttattcatcc gttcctccaa tctgactcca aaccaatcgt
     2641 ccttctacca gtaacccacg aacaaattct cgctggctgg taatatctgt aatgaaataa
     2701 actacatagt cttttccatc ttctggtgcg caataaatat aaagatgtgt catcactaaa
     2761 cgtggtagat gaacatcttc aggaagcata cccatttcaa tttcctcttt ttccatcgta
     2821 acggattcat agccccactc ttcagaagca gtgaccagct gtgcacactt ttccagtact
     2881 tcgatagccg tttctaacaa gtcctttcgt tcctttcttt tagatactag tattcttctg
     2941 gtaataacat cgtttgatac tcacccgaat caatggccca tattttttga gacactgatt
     3001 tcaaacgaag cttaggtgaa atcagatact cttttctttc ttccggttct tcttgacgat
     3061 gaaccaactt ttgatttcct ttttccgtag aaaattcaaa aatttgaaga tagtctaaag
     3121 gaaattgctt ttgaacgttt tgatcgatta gattccaaca taaagcttgt aattccaccg
     3181 gcaaactgtc cattactccc tttgtgacat aacgttatcc tttattttga aacatttctt
     3241 ttccctcctt tgccataaaa atagcccaat cttctattaa agaagattgg gcttcatgga
     3301 tataatatat atttgtaaat aaagttaatt cagttataat tatcaaaact acacaattat
     3361 taaagttttt catatctctt tgtttagtaa gtttttctaa atcttaaccc gtggtgctag
     3421 ttaatttttt cctagaggaa aatgtctaaa catgttggta aatcaacaaa aaaacgcata
     3481 tagtagtctc aaaactccca agaaatgagg actatttaac aaactacgat ggttacgtcg
     3541 tgacaactga acattttgac ttagccaatc ctgttactaa agtcgatgat atccccgatt
     3601 atgaaatgta cagtcaaaca atcgatagct taaataagcg ttttggcaat cgtgttttaa
     3661 aaggcatcaa aatcggttac atcgcttctg aaaaggatcg gatcatcgac tatttagccg
     3721 ataaagatta cgatttaaaa ttactcagtg tgcatcataa tgggcaattt gattatttag
     3781 atgacgaagt caaagatatg gatcctgcta ttgtgattcc tcagtatttt gcacaacttt
     3841 cagaagcact agttgttata gaagcagatg tctttgctca ttttgactat ggttttcggg
     3901 tatttggtct atccgttgct gaatttaaac aatatgaagc gcagttttta ccgattctag
     3961 atcaagtaat caaaaataaa ttggcattcg aattaaatgc taagagcgct tacttgtatg
     4021 acaatttagc gttatatgaa tatgtcatcg atttatatct ttcacgtggt ggtaccttgt
     4081 ttagtgttgg atcagatggg cattaccttg aacatttccg tttccatttc gatgatcttt
     4141 ttgctctgtt aaaagcaaat ggtgtgacgg aattagcgat ttatcaaaaa ggaaagcgaa
     4201 ttatggttcc attaccagtc taaaaaaacg tcctcagcat caaaagtgag gacgtttttt
     4261 tagatttctt tttctaaaat cggacaaata cgatcttgtt ttcctaaagg aaaatgtttg
     4321 atcccgattt ttttaaatcc atgacgttca taaaaacgaa tacctgcatg atttttttca
     4381 taaacagtca aacgtaaata acgtttttgt ttcgttttag cataacgatt cgcttcatcg
     4441 agtaaccgtt gccctaaccc tttgttttga taatctttga gcagatagag ccgttgaatc
     4501 tctatagcat ctttttcatc attgactttc atataaccaa tgagtatttg atgatcctta
     4561 ataccaataa atttactggt tgactgtgcc aattctactg ccaatatttc agttgtcaat
     4621 tgtgtctgta aatatccttg catatcaagt ggatctgcgc catcgataaa cgattcataa
     4681 aaactgacaa cacttacaac ttgtaaagca tctagatcat cataatctaa atacgctagt
     4741 gtgatttgat tgttcatcta actcctcctt ttgacatagt atactataaa t
//
LOCUS       DAFBQH010000118         4755 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.125, whole genome shotgun sequence.
ACCESSION   DAFBQH010000118 DAFBQH010000000
VERSION     DAFBQH010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.125"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            408..674
                     /locus_tag="LK694_002652"
     CDS             408..674
                     /locus_tag="LK694_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK5053465.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            851..2809
                     /locus_tag="LK694_002653"
     CDS             851..2809
                     /locus_tag="LK694_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBK5053466.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            3163..4464
                     /locus_tag="LK694_002654"
     CDS             3163..4464
                     /locus_tag="LK694_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBK5053467.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
BASE COUNT         1434 a          844 c         1036 g         1441 t
ORIGIN      
        1 tttttcgaat aaacggtgtt caaaagcttg catctgcggt aataagtcca gccaagttaa
       61 aaacattagg agctgttttt acttggctgt tcttattact tgatgcagaa cagctttttt
      121 acaacctctc caataaacga tgttcaatca gctgtccctc gatattagct caaaaaacgg
      181 caaaatatga ggaactattt ttccgtttgt ttctctaata ctcaggtcaa accgctgatt
      241 tcacaacctc tgattcacgg tgttccagaa gcagcttcta cggaaataag ctgaacactt
      301 ttgttacaac ttcttttttt ctggagtttc ttggagcata tttttggagt ccttctgaat
      361 aaagcgtaaa ataaaagtga gaaatatgga taaaggaggg aaaggagatg ctgaattttt
      421 tatggtcgtt atttatcggc ggtatattag gtttgatagc tggcgcaatt ttaggaaagg
      481 aagtcccggg aggcgtatct ggaaatatca ttatcggttt tttaggtagt tggctcggcg
      541 aatttttact aggaccactg ggaccggaac tcggcggttt ttatttgata ccagcattat
      601 tgggtgcaat tttttgcctt gctctttatt catttgtggc aagacgtatc agagaacgtc
      661 caaagaaaga ataattttat tcaaataagt tgacacttca gtgtgttact tggataatta
      721 gaagcaattt aggaaagcgg gtgtcatgga taagaatatc atctatcatt caagcttacc
      781 taaaggatat ccatataaca agaaatgagg gaaaaacgtg gcaacaacgc acaataccaa
      841 aaagatatca atggctggcc tgcttgtagc gatgggagtc gtctacggag acatcgggac
      901 aagtccgctt tacgttatga aatcgattgt tgaagataat ggcggattgt caggaatcaa
      961 tcctgatttt gttatcggat cagtctcatt gattttttgg acattgactc tactgacaac
     1021 aatcaaatat gtcgtgattg cattgaatgc agataaccat ggtgaaggtg gaattttctc
     1081 actctacaca cttgtccgaa aaggtgggaa atatttgatc attcctgcaa tgatcggagg
     1141 agctgcctta ctagcagatg gggtattgac ccctgctgtg acggtaacga ctgcgattga
     1201 aggcttgcga ggaattccgc agttttttga ccggttcggt aataaccaga acgtgatcgt
     1261 cattatcact ttagttatta tttttgtttt attcatggtc caacgctttg gtacagatat
     1321 tgtcggaaaa gcattcggac caatcatgtt cgcttggttt acttttttag gcgtaatggg
     1381 attaatgaac ttcagtcaag actggacagt tatccgtgca ctgaatcctt attatgctat
     1441 ccagctgctt tttagtgcag ataacaaatt aggcttgttc attcttggaa atattttcct
     1501 ggctacgaca ggtgcagagg cgctttattc cgatcttgga catgtcggca aacgaaatat
     1561 tcgtctgagc tggccttacg tcaaaatttg tttggttatc aattattttg ggcaggctgc
     1621 gtggttgttg aatgtttatc aaaaccctga agcacagcaa atcaagaacc tgaatccatt
     1681 cttccaaatg atgcctcaaa gttggatcgt gatcggcgtt gcttttgcaa cagtcgctgc
     1741 agtcattgct tcgcaagcat tgattacagg ttcgtttacg ctagtttcag aagcaatcaa
     1801 gttgaaactg ctgcctcggt taaagatcat gtatcctggg aacagtatcg ggcaaatgta
     1861 tatcccagca gtcaatctga ttttatggat cgcctgttct ctagtggttg tcacattccg
     1921 aacgtctcat catatggaag ctgcttacgg tctatcgatc actgtgacga tgttgatgac
     1981 aacagcactg ctttactttt atcttatcca aaatgggcat tctcgttggc ttgcatattt
     2041 agttaccttt ttcttcggtg cgatagaagt cgttttcttt atctcaagtg ttgtcaaatt
     2101 cttccgaggc ggttttgtag ccgttctgat tggtttgatc atcttagtcg tgatgtttat
     2161 ttgggaacaa gggaacatta ttcgcgaatc gtcaaccgag gatgtgtctt tgagagatta
     2221 tatcccgcag cttagagaat tacgtgatga tacttcttta ccaatctatc agacaaatgt
     2281 ggtcttcttg gttcctgata tggctgaaga taaagttggc cgacagttca tctattcgat
     2341 tttagataaa cggcctaaaa gagcacgagt ctattggttt gttcatgtgg aagtgacgga
     2401 tgagccatat acgaaagaat atcaagtaga tatgatggac actgatttta ttgtccaagt
     2461 gaatctgttc ttaggcttca gggtccaaca ggaaatcaac gtatatgttc gccaagtcat
     2521 ccatgatttg atgaaacaag gccgtttgcc aaaacagcca caaagatact ctctgacacc
     2581 agggcgagaa gttggcgatt tccaatttgt cctgattgat gaagtagttt ccaatgtgac
     2641 aactctagga aaatggcaac gtcaaatcat gcaggcaaaa ctggcaatca aaaaaatcgc
     2701 tacctcgcca gaaacatggt ttgggctaga gtacagcgaa gtaaaacatg aaactgttcc
     2761 gcttatcatt ggaaatactc ggaaaacatg gttgaaagag cggttgtgaa agtagcgctt
     2821 tgacctgaac taatgtcgaa tgtcagctac ttgaacaccg tttatcagag gtcatgaaaa
     2881 agctgtactg caacaaacag taagaacgag cagataaaaa cagcttctca tgtttttcat
     2941 ctgtttgggc ttaatgccgc agatgcaagc ttttgaatac cgtttttatc agaggtcatg
     3001 aaaaagctgt ttagcaacaa gtaacgcaat gattttgaag tgataaggag atgtggctga
     3061 aaaatgccgc atctcctttt ttattcctaa atatgaatac gcattatttt caatcttttt
     3121 gtagaaaaag ggtacaatag aaaaagagaa aggcgggaga atatggttat caatttcatt
     3181 attttatttt tgatgattgc aattacttca ttttttgtag caagtgaatt tgctttagtg
     3241 aaaatcagac gatcaagatt ggaacaacta gaaaaggaaa atgtaaagaa cgccaagcta
     3301 gctcttcatg ttacacacca tttagataac tatttatcag ctagtcaatt aggaattacg
     3361 ctaaccggtt tgatcatcgg gtgggtcggt gaaggatctg tcgcggcttt actagaacca
     3421 gtgatcggta aactaccttt tagttcggct attagcagta caatctctgt ggcgctggga
     3481 tttattttag ttacgtatat cgatgtggtt attggcgaac tgcttcctaa gagctatagt
     3541 atcgtcaata cggagaaggt cgtcttgttc gtagttaaac cacttcatta tttttataaa
     3601 gtaatgtttc cttttatttg ggtattgaat cattcagctg cagggctagg gaagctatta
     3661 ggtgtgcgtc tcgtatctga aggagaagag acattgtctc aagaagagct gacattagtc
     3721 gcattgaatt cttatgaaaa aggagagttg accaaagaag aatatcatta tttggaaaac
     3781 gtgtttgaat ttgatgatac cttggcaaaa gatatacaag tagaccgtac gtccatgcaa
     3841 gtattcgaag cttcagaaac cgtcaaagaa gcgataaaag cttcgattga acaaggacat
     3901 actcgctatc cggttatctt agaatcaaaa gacaatgttt taggttatgt caccttgcca
     3961 gatttgatca agcaatctta taaagatgac cagctgacag tagaacagtt gattgaagag
     4021 ccgatcgtaa caactgaaac gatccctata aaaaaattat tgacgatcat gcgaaaaaag
     4081 ggaaagcata ttgctatctt aaaagatgaa tacggaggga caagcggctt agtgacgatt
     4141 gaagatattt tagaagaaat cgtcggggaa atccgagatg agacagattt ggacgaagca
     4201 ttgatagcgg aacagtcaga tggctcttat attatctccg gcaaactaac attagatgat
     4261 ttccagcgtt attttcatgt agagattcca gagtttgaag aaacgaattt tactacactt
     4321 gcgggctttg cttctagccg atataaagaa ataaaagcag gaacaatcat tgagattgca
     4381 tcattccgtt tcacggtatt agaataccag catgcacata tcgattattt caaagtagag
     4441 tccacggaaa gaaaaacaga ataaaagaaa aaaggctgtg acattttttg tcacagcctt
     4501 tttcctacga ttagtcaagt aaaaggtgca agaaaaatag ctcgtaaacg aaagcgacaa
     4561 aatcaaagca caccaaataa agccccgttc gtaaaaaatt ttcgatctgg gcaaagaagt
     4621 ggtatactgt aagtggttta gttaatggtc cactgagacc gcgtacgcat agtcgtactc
     4681 tgtatgtgcc ctaaccatag cgtgcataca tttcagaagc ttgttcatac aagcaactgt
     4741 ggcaacctta tcttt
//
LOCUS       DAFBQH010000119         4726 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.126, whole genome shotgun sequence.
ACCESSION   DAFBQH010000119 DAFBQH010000000
VERSION     DAFBQH010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.126"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            130..1275
                     /gene="tgt"
                     /locus_tag="LK694_002655"
     CDS             130..1275
                     /gene="tgt"
                     /locus_tag="LK694_002655"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBK5053468.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1372..1752
                     /gene="yajC"
                     /locus_tag="LK694_002656"
     CDS             1372..1752
                     /gene="yajC"
                     /locus_tag="LK694_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBK5053469.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKKEK"
     gene            2057..4654
                     /gene="adhE"
                     /locus_tag="LK694_002657"
                     /gene_synonym="adhC"
     CDS             2057..4654
                     /gene="adhE"
                     /locus_tag="LK694_002657"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBK5053470.1"
                     /translation="MAKVMEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     AQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         1655 a          834 c         1010 g         1227 t
ORIGIN      
        1 ataaaaaaag gataaaaaaa agaatattaa ataaaataga aggaagccga gggaacttgc
       61 aaaaagcact tgaaacgatt atgatggtgg gagttgtcaa gatattttat atagaaagta
      121 gggaaatgaa tgacgcaacc tgccattcgc tatcgtttaa tcaagaaaga aaaacatact
      181 ggcgcgcgtt taggggaatt gatcacgcca cacggaactt tccccacacc aatgttcatg
      241 ccagtaggaa cattagctac agtaaaaaca atgtccccag aagagttgaa agaaatgggt
      301 gctggcgtta ttttaagtaa tacatatcat ttatggctac gccctggtga agacttagta
      361 gaagaagctg gcggactgca taaatttatg aactgggatc aaccgatttt aacagattca
      421 ggcggctttc aagttttttc attaagtgat atgcggaata tcgaagaaga aggcgtgcat
      481 ttcaaaaatc acttgaacgg ttcgcgtatg tttttatcac cagaaaaagc aatcaacatc
      541 caaaataaat tgggctctga tatcatgatg agttttgacg aatgtccacc attcgatgaa
      601 agttacgact acgtgaaaaa atcagtagaa cgtacgtctc gatgggcaga acgaggattg
      661 aaagctcatg ctaacccaga ccgtcaagga ttatttggaa tcatccaagg agcaggattt
      721 gaagatcttc gtcgtcaaag cgcgaaagat ctagtcggga tggatttccc tggttactca
      781 atcggcgggt tatctgtcgg ggaacctaaa tcagaaatga atcgtgtatt agaattcact
      841 acaccgttga tccccgaaaa caaaccacgt tacttgatgg gtgtcggtgc tgctgattca
      901 ttgatcgatg gtgttatccg tggagtagac atgtttgact gtgtcttgcc tactcgaatc
      961 gctcgaaatg gcacatgtat gacttctcaa ggtcgtctag tagtgaaaaa tgcgaagtat
     1021 gctcatgatt tccgtccaat tgatgagaaa tgtgattgtt atacatgtaa aaactataca
     1081 cgtgcctata tccgccattt gatcaaatgt gatgaaacat ttgggattcg tttgacttct
     1141 taccacaatt tatacttctt gttgaatcta atgaaacaag tacgtcaagc aatcatggat
     1201 gataatttgc ttgaatttag agaagccttt tttgaagaat acgggttcaa taaagaaaac
     1261 gcaaaaagct tctaaaggaa aatcttttaa aaattaagta aaaaactagt gttagcagca
     1321 tttttctgct aaagtgtttt ttgtagatac agaattagtg aggtgaaaaa aatgggaaca
     1381 ttaccaatgc ttgtgatgtt tatcgcgcta ttagcaatgt ggttttttat gtcacgttcg
     1441 caaaagaaac aacagcaaga acgtcaaaac ttgttagata gcatgaaacc aggtgattca
     1501 gtcgtaacga tcggcggatt acatggagtt gtttcagaaa tcgacaatga caaacggaca
     1561 gttgttttag actgcgaagg gatcttcctt gagtacgatc gtgcagcaat caaaacggtc
     1621 aaaccaggga ctgtcgtaac aaatgatgca acaactactg ttgaaacaac agaagaacca
     1681 gctgagacag ttgaagtaaa aactgaaaca acagaaacgc tggaagagac agaagagaag
     1741 aaagaaaaat aagtatcaaa gcttatttaa agacgaggta gggcagaaag atgtcctacc
     1801 tcatttttta ttcaaaaaag aaagagataa gagtatagga tatattgctc aatattgaat
     1861 aatcgggtgt gtcatataaa taataataaa taactttata tatttttcta tacaaacgcc
     1921 tataaataaa gcaactttat atttaaaaat aactgtgaaa aaaatcacaa aaaagtgttt
     1981 acatagaaaa ataaatattg tatgatatac atgtgaatga tgtaacaaat aaccatgacc
     2041 cattaggagg ataatgatgg ctaaagtaat ggaaaaagaa aaaacgaaga caattgacgt
     2101 acaagcaatg attgatgatt tggcagaaaa agcaaatgca gcattgaaag aaatggaaag
     2161 ctttgatcaa gaaaaagtgg accatatcgt gcatgaaatg gcaatggcag ctttagatca
     2221 acatatgcca ttagcgaaga tggctgtaga ggaaacaggc cgcggtgttt acgaagataa
     2281 agcaatcaaa aatatgtatg cgtccgaata tatttggcat aatatcaaac atgataaaac
     2341 agttggtgta atcaatgaag atgcccaaaa aggattgatt gaaattgctg aaccagtcgg
     2401 tgtggtttgc ggagtaactc caacaacgaa tccgacttcc acaacgattt tcaaatcaat
     2461 gatcgcgttg aaaacaagaa atccaatcgt ttttgctttt catccaagtg cccaaaaatc
     2521 ttctgcagaa gcagcacgag tagtacgtga tgctgctatc gctgccggag ctcctgaaaa
     2581 ttgtatccaa tggatcgaac atccttcgat cgaagcgact tctatgctaa tgaaccatcc
     2641 aggtatcgcg atcgtcttag cgacaggtgg agctggaatg gtcaagtctg cttactctac
     2701 aggaaaacca gctttaggtg taggtccagg taacgtacca gcttatattg aaaaaacagc
     2761 aaaaattaaa cgtgcagtga acgatttgat cgtttcgaaa acatttgata atggaatgat
     2821 ttgtgcttct gaacaagctg tgatcgtgga taaagaaatc tatgcagcag taaaagcaga
     2881 attccaagca caccaagttt atatcgtaaa accagatgaa ttgcaaaaat tagaagatgc
     2941 agtgatgaac gaaggaaaat atgcggtcaa tccatcaatc gttggacatt ctgcgatgga
     3001 aatcgcaaaa ttggcaggaa tcagtgtacc aaaaggaacg aaaatgctga ttgctgaatt
     3061 ggaaggcgta ggcccagatt atccgctatc aagagaaaaa ctttctccag ttcttgccat
     3121 gatcaaagct aacaacacgg accatgcgtt tgatttgtgt gaaggtatgt tgaatcttgg
     3181 aggattagga catacagcag ttatccattc agaaaacgaa gagttgcatg tcaaattcgg
     3241 tcttcgaatg aaagcttgtc gtattttagt gaatacacca tctgcagaag gcggcatcgg
     3301 tgatatctat aacgaaatga ttccgtcatt gactttggga tgcggttctt atggtaagaa
     3361 ctctgtttct cgtaacgtct ctgctgtaaa cttaatcaat gtcaaaactg tagcgaaacg
     3421 gagaaataac atgcaatggt tcaaattacc gccaaaaatc ttctttgaaa agaactctct
     3481 attgtactta gagaaaatgg aaaacgttga acgtgtcatg atcgtttgtg accctggtat
     3541 ggtccaattc ggttactgcg atacagttcg tgaagtattg gcacgacgta aaaatgatgt
     3601 gaagatcgaa gtcttctcag atgttgaacc gaacccatcg acaaataccg tatacgcagg
     3661 aacgaaactg atggcagact tcaagccaga tacagtaatc gctcttggag gtggttctgc
     3721 aatggacgca gctaagggaa tgtggatgtt ctatgaacac ccagacacat ctttctttgg
     3781 tgcaaaacaa aaattcttgg atatccgtaa acggacatat aagatcgaca aaccagaaaa
     3841 aacacaattc gtatgtatcc cgacaacatc agggactggt tctgaagtaa cgccatttgc
     3901 ggtcatcact gacagtgaaa cacatgtgaa atatccgtta gcagattatg cattgacgcc
     3961 agatgtcgca atcgtcgatc ctcaattcgt catgtcagtt ccagcatctg tcacagcaga
     4021 taccgggatg gatgtgttaa cacatgcaat cgaatcctat gtttcagtca tggcttcaga
     4081 ttatacacgt ggattgagcc tacaagcaat caagttagtc tttgactatt tagaaaaatc
     4141 agtaaaaaca cctgatatgg aatcacgtga aaaaatgcac aacgcttcta caatggctgg
     4201 tatggcgttt gctaacgcat tcctaggtat ttgtcactca gtcgcacata aaatcggcgg
     4261 ggaatacggg atccctcatg gacggacaaa tgcgattctt ttaccgcata ttatccgcta
     4321 taatgcaaaa gatccacaaa aacatgcgat gttccctaaa tatgactatt tccgtgcaga
     4381 tacagattac gcagatattg caaaattctt aggtctgaaa ggcgaaacaa cagaggaatt
     4441 agtcgaagct ttagcaacgg ctgtttatga attaggaaaa tctgtcggca tcaacatgag
     4501 tctaaaagct caaggagtca cacaagaaac actagataca accgtagacc gtatggctga
     4561 attagcttac gaagaccaat gtacgacagc taatccgaaa gaaccattga tctctgaatt
     4621 gaaacagatc atcattgatg cttacaacgg ataagcaaaa gaaagcgtag aaaaaagata
     4681 agaaaataga tgaaaaagaa cttggaaaac gttgtttctc caagtt
//
LOCUS       DAFBQH010000120         4655 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.127, whole genome shotgun sequence.
ACCESSION   DAFBQH010000120 DAFBQH010000000
VERSION     DAFBQH010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.127"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            248..523
                     /locus_tag="LK694_002658"
     CDS             248..523
                     /locus_tag="LK694_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053471.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            536..2614
                     /locus_tag="LK694_002659"
     CDS             536..2614
                     /locus_tag="LK694_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBK5053472.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2774..>4655
                     /locus_tag="LK694_002660"
     CDS             2774..>4655
                     /locus_tag="LK694_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK5053473.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVE"
BASE COUNT         1553 a          745 c         1004 g         1353 t
ORIGIN      
        1 cagccccatt tttttgttgt gataaaagaa caagaaaggc aggaatggga aagtaaacat
       61 ccttttgtca gaaactttta tctttcttaa gttttacttg atatctgata ctatttggaa
      121 tagaatgagg cgttgaaaaa tttatggaaa tttctaacta gcaataaacg atgaaacaat
      181 gaaatatttt tcatccaaaa agaactttgc tataatgatg gaagtttgtg tataggagat
      241 ctggaaaatg agagagagtt ttgaacaaca aaaaaaactg ttgcatgatc gctatggcgc
      301 cctttcgatg gatgaccgcc gtcaaatact ttgtaagttg cgtaaaagaa atatattgat
      361 gtatcgtcag ttggaacgtc tgaaacatga tctgcttcgt ttggagtcaa aacgtgtaca
      421 gtgtgaatta gaggggaacc aaacacaggt tgaagtagtc gaaaccaaaa ttttaaagaa
      481 aaaagaacaa tttttaaaaa tgcttacaca aaataaaaaa taaaggggat ggacgatgga
      541 atttgctgag ttagttattg tatttgcagt aacgatcacc ttatcgaaca tagctagccg
      601 cattttgcca atgattccag ctccactgat ccaaattttc cttggagtgg tattaggtct
      661 gacagagtgg ggacaatcaa tcgattttga acctgaattg ttcttagtta tgattattgc
      721 accattgtta tttagagaag gagaaaaagc agatatttct tcgattctca aaaatttcgg
      781 gacaattttg ttcttggcat ttggaggagt cattttgacc cttgtcgggg taggtgcgac
      841 gttatcattc ctgttgccaa gtgtcccact tgctgcctgc tttgcttttg gagcagcgtt
      901 aggtccgaca gatgcggtag cagtcagttc tctttctgga cgagtgaata tccctaaaaa
      961 ggcgatgcat attttggaag gagaaggtct tttgaatgat gcatctggtg ttactgcctt
     1021 ccagtttgct cttggtgcgc tgatcaccgg atctttttca gcagtaaacg cggggatgag
     1081 tctagtagtt tctagtattg ggggagcttt gatcggcttt ttgcttgtct ggttcaaaca
     1141 aaaaataatt catttgatcg aaaaagcatc agcacaagat gtcacagggt atctgctgat
     1201 cgaacttttg cttccttttc tagcctatgt attggcagag gcatttgatg tatctggaat
     1261 cattgctgca gtggcagcag gtattctcca agcttctggt tttaggaaaa tctctgtgtt
     1321 tgatgcagaa ttgtcgagct tgtcccacag tacatggaca actattgctt ttacactgaa
     1381 tgcattagta tttatatttt taggaatcga attgacgcaa gtgttctcgc cagtatgggg
     1441 agacggttta tatccaaacg gtcttttatt agcgattatc gtattaatct ctgtcatgct
     1501 ttttgtgatc agattcattt ctatcagtct tttttatgta ttcaaagatg gcagtaaaaa
     1561 gttcaaaaaa cagttgaacg agatcttgat cttaacattt ggcggtgtaa agggaacagt
     1621 cagtttggct acgattttta ttttgccatt ttcaatcaat aatatgatgt tttaccagcg
     1681 gtctttgctt ttgtttctga cggcgggtgt catcttagtg actttggtca tagggatcat
     1741 tgtcttgcct atgctgacgg aaacagagga agcgaaaagc acggatttga atgctttgat
     1801 gattttagaa gaggtcgttg aagttttgcg aaaagaaata aaggaaattg atcgtaatac
     1861 aaaagaattt ttagccactg aagcagtgat agaaaattat caagaacgga tacgtgatct
     1921 gtacttggaa gatttaaccg atgatgaaaa gcaagaagta caagaaatac aagcactgat
     1981 tttgtcgatc gaacgggatg gattggatga aagttatcgt tcgggcaagc tttcttctaa
     2041 tggttatcgt ttttactccc gttttctttc tcgattcgaa cattctatca caagccagat
     2101 tttgtctttt atcggctttt ggttcattgt tgtccgtagg ttaatgcgaa tcgttctcca
     2161 tccgaaaatg ttttggcaaa gaagatatgc tgatcgtcag acatttatta gtgatgaaga
     2221 tatccaagaa atcagagaga catatcaaaa aaatactcag ttgattattg aaagtttaga
     2281 taacttgact gatatttatg atgaaacgat ggttcaattt tttatccagc aacgcaaact
     2341 tgaaggaata aaaatgaact cggggaattt gatttcttcg tggatgatcc agcaagatag
     2401 tctgtttacg aaaaaaatgc taagaggtta ttatcttgaa agaaaaatca ttgatgaata
     2461 cgaagttgct gaaaaaatca cgacgttttc tgcgaacagc tatcgcagga atatcaattt
     2521 gttagaatct tatacgatga acaaaccatc tgataacttt tctttccgat ttgcgtttcg
     2581 agcaaagtca aaaaaaacga gtccaataaa ataaaaaggt aaaaattttc aagttttata
     2641 aaaaacaaaa catatcagaa caggatcgaa tcggtcctgt tctttttttg agaacacaaa
     2701 aagaacaaat accaaaaaaa ttttcctgaa gttcactatt tttatgatac aatatccaag
     2761 ataaggaggg aacatgttga aagaattaaa agtaactgac ctcaaaaaaa cttatggtga
     2821 aaaagatttg tttgaccaga tttctttttt ggtccacgaa aaagaccgta tcgggttgat
     2881 tgggacgaac gggacgggta aaacaagtct cttaaatatt attgccggta tcgatagcag
     2941 cgacggtgat cggcaaacag tgttttatcc aactgattac cgtattggtt atctttctca
     3001 agcagctgaa ttttccgatg aattaactgt tctccaagca gtctttcaag gaaacagccc
     3061 cttgatccaa actgttcgag catatgagga agcattgatc gaactagggg aaaacggaga
     3121 agaccctgat gtgcaaaaac gttatgctaa agcagaagaa caaatgaata aagaagatgc
     3181 ttggactaca gataccaacg caaagattat tttgcaaaaa ttaggaatca gtgaattaga
     3241 taaaaaaatc agtactttat caggtggtca gaaaaaacga gttagtctgg cgcaagtgtt
     3301 gattgatgaa cccgatttac tattactaga cgaaccaaca aaccatttag attatgaagc
     3361 gatcgagtgg ttggaaaact atttgaaaca ataccgtggc gcactattga tggttaccca
     3421 cgatcgttat tttctagata gagtagcaaa ccgaatcttc gaactttcat ttggaaaatt
     3481 atacgagtat aaaggaaatt atgaagctta cgtcttagaa aaagcagaac gtgatcgtgt
     3541 agaagtagaa caagaagaaa agcgcaagcg gctttataaa caggaattag cttggatgag
     3601 ggctggcgta caggcacgtg gcaccaagca gcaagcacgg atcaaccgtt tcgaagatct
     3661 aaaggagaac ttgtaccaag taaaccaaga agatgattta gagctgaacc tagcgacaca
     3721 acgtttaggg aaaaaagtac tggaaatcaa agatggcagt taccgtataa atgaccaaac
     3781 actgctagaa catttagatt tattgattca atctcgggaa agactaggta ttactggtaa
     3841 aaatggtgcg ggaaaatcga cattgcttaa tattctagct ggcagaattc ccctggatag
     3901 cggtacaatg tctatcggtg aaacggttca tcttgcgtat tatacacaag aaaatgaaga
     3961 aatggcacca gataaacgga tgattgctta cttacaagaa gctgctgaag aagcaaaaac
     4021 agcagatggc agtcaaatcg gagtagcaga attacttgaa cgatttttat ttccaagatt
     4081 tatgcatggg acactgatag gcaaactttc aggcggagaa aaacgccggt tatttttgtt
     4141 gaaactgctg attcagcaac ctaatgtttt attattggat gaaccgacca atgatttgga
     4201 catagcaaca ttgacgattt tggaagatta ttttagaagt tttccaggtg ctgtgatcac
     4261 tgtttcccat gaccgttatt tcttggacaa ggtagcagat aagttattag tattccaagg
     4321 aaacggaaaa caagaacttt attacggaaa catgtccagt tatttactga agcaaaaaga
     4381 gacccagcag cctgctgaaa aagcgaaacc aaaaatacag agcaaggaac ctgctgaaaa
     4441 gaagaaatta tcttacatgg aacaaaaaga atgggaaacg attgaagatg agattgcaga
     4501 acttgaagaa aaaatctcat tgctccaaga agagatgaat catcaaggag ataatttcac
     4561 tcgattacaa gaattgcaaa acgacgtatc tgaaacagaa gcacaacttg aagaaaaaat
     4621 ggcacggtgg gaatacttga gtgaatgggt agaag
//
LOCUS       DAFBQH010000121         4554 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.128, whole genome shotgun sequence.
ACCESSION   DAFBQH010000121 DAFBQH010000000
VERSION     DAFBQH010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.128"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            155..2824
                     /locus_tag="LK694_002661"
     CDS             155..2824
                     /locus_tag="LK694_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBK5053474.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2915..3535)
                     /locus_tag="LK694_002662"
     CDS             complement(2915..3535)
                     /locus_tag="LK694_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK5053475.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3647..4048
                     /locus_tag="LK694_002663"
     CDS             3647..4048
                     /locus_tag="LK694_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053476.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANS"
     gene            4183..4521
                     /locus_tag="LK694_002664"
     CDS             4183..4521
                     /locus_tag="LK694_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HBK5053477.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1414 a          784 c          852 g         1504 t
ORIGIN      
        1 ctttttttgc gtttattttt gttatacgaa caaattatga agttatttat tctaaatttt
       61 atttttttgt gaaaattaga ctctttttaa gcagagaaaa acaaggatat gataaactga
      121 atacaacttc atagaaattt ggtggaaaat aaatatgaaa aaaaatttaa agtcattctt
      181 tagagaaaac ggacgctacg cagcagctag tttcctgatt ccgtttttga ttttagcact
      241 tgtttatcta agtatcggga tctatcctgg aagcagcaga agtattttgg caagtgatgc
      301 cttttcacag ttttctaatt ttcatgccag cttccgaaat gttcttcttg gaaagcaaag
      361 tattttttat acatggaatg cttctctagg attgaattat ttatctttaa tttcttacta
      421 tctaggcggg atcttcacac cactggtgtt cttttttcca aaccagatga tgccagatgc
      481 actgtatgtt ctgactttgc ttaaggtcgg ctgtgccgga ttggcttttt ggtttcttgc
      541 acgtacgttc aagattcctc gctgggggca tgttgcactt agcgtcagtt acgcattgat
      601 ctcatttatc actgcacatt cggagatcat catgtggctg gatgcatttg tttatcttcc
      661 attagtgatt ttggggattc atcgtgttat ggatcttaaa cggccgaaac ttttatttat
      721 cagttattta ctcttgtttt tatccagttt ttatatgggt tttatgattg gcgtattttc
      781 tttcttatat tttatcgccc gtctattgac aaactggtca gaatataaaa aaacgattgt
      841 tccttatggt atcacttctt tactggcagg cggcgcttca atgatcattg tattgccagc
      901 agtgctggat cttcggtcaa atggcgaggc actgagtcag atcactacgt tgaaaacaga
      961 agcgacttct tatctggatc taatcatgaa aaatatgatt ggtgtttatg acacgacaaa
     1021 atacggatcg attccgttcg tttacgcagg tctgctacca ttagtcctct gtctgtttta
     1081 ttttgccagt cggaaagtac cgttgaaaaa taaatttttg tacggcagtc ttttcgccat
     1141 acttatcgca agtttctatc ttgttccatt gaatttgttc tggcatggta tgcatgcacc
     1201 gaatatgttt ttattccgtt atgcctatct gttctctttc ttagtgctcc tgcttgcagg
     1261 aaaaggatgg gaaaagatag aacaagacga tcgtgggctg ttgttaggaa tcattttggt
     1321 cgtagctgct ctctttgcgg tggcatgggg catcaaacct gaaggaagct acacctatgt
     1381 gacgatgact tcctttgtat tgacagtggt tttcttaggg atttatgctt ttgctgtggg
     1441 attttatcag cataaaaaac tttcattcaa tcatctgagt atattgttat tattgatcat
     1501 gtctgctgaa gcactggtca atacgaacag tatgatccat ggcattcttg atgattggaa
     1561 ttatgcttcc cgcagtttat atactgatcc ttatcctgac ttgaaagctc ttgtaaatga
     1621 aacaaaaaaa gattccgact cgttttaccg cttggaaaat ctggatccag tatctgcaaa
     1681 cgattcgatc aactatggtt acagtggtat cagtttgttt tcttctataa gaaatcgtaa
     1741 ttcttcctct tacctggata aattaggttt ccgttcaaga gggacaaatt tgaatatccg
     1801 ttataacaat aatactttgc taatggatgg gtttacagga atcaaatata acatttctaa
     1861 aaacgaaagc acgttcagta aatatggctt ccgccaagct ggtcaaagtg gcgactatac
     1921 actgtatcag aatgtcaatg cattgccact agcatttact gcaccgttgt ccgttaatga
     1981 tgttgagcag ccagtgacag ataatttgac cagtcagaca aacttgatca atacattgtc
     2041 tggtttaaat gaacagtatt ttacattcta tacacctact ttgaagaatc agttgaatac
     2101 gacgatcaca aagacaacga ctggcgtcac ttactcagaa acagcagata accagccaaa
     2161 agagctcact tgggaagtcc atgtgcctgc aaatacacaa gcttacttaa gtttgttccc
     2221 aaccaatttt gcacaattag aaagttctag tgcgacgatc actgtcaatg gtacatcgcg
     2281 aaaaacgcaa atcaacattt ctggtcaata ctatgatctt ggctattatc ctcaagagac
     2341 aacagtgaca tttactgcaa gtttttatgg aacgaaagaa gtaagtttca tggagcctaa
     2401 agtcgttggt ctagatgttg tagcctatca agccgcaatg aatcagatca aagaaaaagg
     2461 tgtcgaaatg gaagccactg gcagaaaagc ttctgggact gtaaataccg ctgaagattc
     2521 catgttggtg acaactattc cttatgacaa aggatggaaa gctaaaattg atgggaaacc
     2581 agtcaaggtc agcaatttca aagacgcatt cttgatgata aaagttccag caggtaaaca
     2641 tactgtcact ttcagttact tgcctgaagg atttacgata ggtgcaatcc tttttgtcct
     2701 atgtattgca ttgtttattc tttatgtctg gtaccttgac cgcaatcgcc ccttttatgg
     2761 cacgattgat ccagcaatag aagaaaaacc gcgaaataca cacagaagaa gacgaagaaa
     2821 ataaagaaac gatggaatct gtgtcactct tctatttaaa tggcaaaaat ttcaatcttt
     2881 aaatgacaaa aaccacctga tttttggtgg ttttttatat acctatgact aaactatctc
     2941 ttgctgcttc gacaacttct acagtgatct tatccatttg attgattctt aaaatccgat
     3001 caatctgcgc aaataaacga tgaataagcc taaaatttcc ttttgtgatc ttaatgatag
     3061 ttgtgatagc ctcataatca gaaaaatctt ctagcttgat atcaattcct aaatctgccc
     3121 atttgtattc caaaatatga tgtgtttcgt ccttactaag gttatcaaat tcatgtgcaa
     3181 atcctatgcg agagtaaagc tgcggataac gagacaatct tttttcaata ccaggcatac
     3241 cgataagaac cattgccaga tcatttctat cgtaaatatc tcttagctgt tctaaatgct
     3301 gaagctttaa acgatcaatt tcatcgacaa taattaagtc aattgcttca taggttttag
     3361 gcacaggagg atcttcttga ttgtatttta gtaccatatg cattgcttta gtcagaccta
     3421 ttctactagt tattgtgtta atatccgaac tgacacgaga catcttttca gctggtacag
     3481 tataaaaaac tttatttaca cttaatattt catctgttgc atttatcaag aacaagaaaa
     3541 agtctgaata ttcaaaaaat aacggtgtac atttttaaaa ggagatgtat aatcgagatt
     3601 tagaaagcgt tttcctaaaa atttaatgaa ttaggaagtg attactatga aaaaattatt
     3661 tagcgcactt tttattctgt tatttggctt tattggtatt gggacttctg gaaatatagt
     3721 taaagctagc acgatggttg aacaaacaag tgaaatggca aaaactttta gtcaattaac
     3781 accagaagaa caaagttact tcaaatccaa aggatttagt gataaagata ctttttacac
     3841 tcaaaagata gagcctcaag atccgaaaac acgtttttct gcaaatgtat tgcggttaac
     3901 cgcatctact aaaaaaatta ctaatacgca ggggcggaca gaatatatta taacaagtcc
     3961 caattcggcg atgtatcgta tttctacacg cttgagttta ggtagtgcaa aatcatatac
     4021 tcaaacagta aatctagcta attcttaagg ttatggtgga ggaatgtatt ttacatttac
     4081 tgggaaaaga caatattaca gtgtaaaatt agctatgcaa gtttataata catttggtgt
     4141 agggtcagtc tcttgcactg cgggaggatt gacaatagga aaatgataat ttcttactat
     4201 gtcttaaata gaagtgatga tgaaggactc ttcgtttttc aatcattaca tgaggatacg
     4261 atgctaaaag agttttataa tcggattaag aataaacaga tagaaacaaa tttatatttt
     4321 ttaagtgact ctatcttgcc ttttattagc aaccctaaaa ttcttaactt gttgcagaca
     4381 aaagatttga aaagtttacc gattattgtc attgatgagg aaattgtaaa atatggcgct
     4441 tatctaagcg tagaagaatt agaaaaatta actaacattg gaatgagtat tcaggaggat
     4501 aatggggtac atggaacata gaatgaatat gcacagttga gaacggaatt tgac
//
LOCUS       DAFBQH010000122         4433 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.129, whole genome shotgun sequence.
ACCESSION   DAFBQH010000122 DAFBQH010000000
VERSION     DAFBQH010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.129"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(543..2048)
                     /locus_tag="LK694_002665"
     CDS             complement(543..2048)
                     /locus_tag="LK694_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK5053478.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="LK694_002666"
     CDS             complement(2045..2731)
                     /locus_tag="LK694_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK5053479.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="LK694_002667"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="LK694_002667"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBK5053480.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DAFBQH010000123         4415 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.130, whole genome shotgun sequence.
ACCESSION   DAFBQH010000123 DAFBQH010000000
VERSION     DAFBQH010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.130"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(12..482)
                     /locus_tag="LK694_002668"
     CDS             complement(12..482)
                     /locus_tag="LK694_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBK5053481.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="LK694_002669"
     CDS             complement(641..1099)
                     /locus_tag="LK694_002669"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBK5053482.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="LK694_002670"
     CDS             complement(1270..2415)
                     /locus_tag="LK694_002670"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBK5053483.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="LK694_002671"
     CDS             complement(2428..3345)
                     /locus_tag="LK694_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBK5053484.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPNGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="LK694_002672"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="LK694_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBK5053485.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1358 a          826 c          842 g         1389 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aaccattagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagag
//
LOCUS       DAFBQH010000124         4215 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.131, whole genome shotgun sequence.
ACCESSION   DAFBQH010000124 DAFBQH010000000
VERSION     DAFBQH010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.131"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            624..3263
                     /locus_tag="LK694_002673"
     CDS             624..3263
                     /locus_tag="LK694_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK5053486.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3431..4129)
                     /locus_tag="LK694_002674"
     CDS             complement(3431..4129)
                     /locus_tag="LK694_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBK5053487.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIAAILLIMIPI
                     ILFATTIYDGSASVSDATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVIGLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
BASE COUNT         1278 a          805 c          911 g         1221 t
ORIGIN      
        1 ttctttctgt tctgtccaaa ttattctaag atttctcttt accaacatca tttgacaaag
       61 agccagaaaa agccgagctc tcgataagct cggctttctt gtacctcgga aggattcaaa
      121 taaacagaaa tgattgtaac tttccatcat acatgcaaag cacggtgttg aatttcttca
      181 tgttaaacaa aaattttgtt ttcgttacat cttcaacatt tgaaacatct cttgttccta
      241 ttcaattttc agacgtttac gttattcaat tgttacgtat cgcattactt cttgtttcgg
      301 atacttcgtg atccaaacat ccatacgcat tcactattta taggtttgta ctctacacgc
      361 atgtctgggt gaaacctgta cttctaacaa ctttatttgc ttacactgtt ctatcgagaa
      421 attcttctgt ctatttgatg gtatcctcct tccttacatc tatataatag catcctttgg
      481 tagcgtttgc aaatataagc actttaaatt ttacgctttc ttaacaaata atcgataagc
      541 cctttatttg aagtaaataa aaaaaggatg ttatagtaaa ataagtacct ttttttgtac
      601 gaagatttta aaggaggaat aggatggaag aatacaagca gacaatagaa gaagtaaaga
      661 aggtaacaga agcagatcca gatactgggt tgtcttcaca agaagtggat gaacggcgaa
      721 aaaagcaggg attgaataaa ttcgatgagg cgccgaaaga atcgatgatc aaaaaattct
      781 ttcgaagctt atccgatttt acaacgatca tccttcttgt agcagcggtg atttcctttt
      841 ataccgcatt tgctacagaa catggggatt tatttgaagg actgctgatc atcgctatcg
      901 ttgtgatcaa ctcggtattg gcgattgtcc aagaaggaaa cgcagaaaaa gcactggaat
      961 ccttgcaaga tatgaataaa caaacggcaa ctgtcatacg agacggcaaa gtgaccactg
     1021 ttgagtcaga gcaactggta gtgggtgata ttcttgtttt agagtccggt gatgctattt
     1081 ctgccgatgc ccgtttgatc gaagcatcac aattacgtgt agaagaatcg gcgttgactg
     1141 gagaaagtga agcagttgaa aaagatgcag catttattgc tgaagaagat gagtcattag
     1201 gtgaccaact taatatggta tttaaaggat gtacggttgc tgcaggtcgt gggaaagcga
     1261 ttgtcactgc cattggtatg gctacagaaa tgggtaagat cgcggatctt ttgaacgaaa
     1321 atactatgca aaaaacacct ttacaggtcc gattgaatca gctaggcaaa cgaatcagta
     1381 tgatcgcttt agcggctgcg gctttagtat ttgtgattgg tgaactgcaa ggagaaccac
     1441 tccttgagat gttcatgact tcgatttcct tggctgttgc agctgtaccg gaaaccttga
     1501 ctgttattgt tacgttgaca cttgcatacg gtgtgcagaa gatggcgagg aaacatgcga
     1561 tcatccgaca gttgccagca gttgaaacgc taggaaccgc taatgttatc tgctcagaca
     1621 aaacaggaac gctgacacaa aatgaaatgc gcgtccgccg ggtatggagc aaagaagatg
     1681 aagtaacgaa tatcgaagat tcaatgacga acagcgcgat ggaaatattg aaaatggctg
     1741 cactatgtac agatgtgacg gtcgagcaag aagatgacga ccttgttatc aaaggtaatc
     1801 caacagaagt agccatcgta agggctgttg aagagaatta tcatacaaaa gcagaattag
     1861 aagaaaaata tccacgagta aacgagcttc cttttgattc tgaacgaaaa atgatgacaa
     1921 cagttcacca aatgggcgaa aaatacatct caatcaccaa aggagcattt gatgttcttg
     1981 ctccgcgctt tagttcaggc gatgtagaac aagcggcgat cgttaatgat cgctttggta
     2041 aacgggcact gagagtgatt gctgttggtt atgctactta tgacgaagaa ccgcaagata
     2101 tttcttctga agcactggaa aaagacttac gtttgattgg tctgatcgga atgatcgatc
     2161 caccacgtcc agaaagtaaa ggagcaatca agcgagcgaa aaaggctggc atcaaaacag
     2221 tcatgatcac tggagatcat gtggtaactg ccagtgccat agcgaaagaa ttaggtatct
     2281 tgaatgatcc gtcagaagca ctctcaggat cagaactgca tcaattatca gacgaagaat
     2341 tagatgcgcg tgtcaaagct ctctctgtct atgctcgggt aacgccagaa gacaagatcc
     2401 ggatcgtcaa atcttggcaa cgtacgggtg cagtcgtagc gatgactggg gatggtgtga
     2461 atgatgcacc agcgttgaaa gcaagtaacg ttgggtgtgc catggggatc acagggacag
     2521 atgtggcgca aagtgccgca gatatgattt tgacggatga caactttgcg acgatcgtag
     2581 atgctgttag tcaagggcgt tcagtttatc agaatattcg taaagcaatc aactttttac
     2641 taagctgtaa tatttcggag atctttattg ttttgattgc gatgctgtta ggatggggag
     2701 caccatttac agctgtgcaa ctactgtttg tcaatgtagt tgctgatgga cttccaggat
     2761 ttgctttagg aagagagcca gcggaacacg gcatcatgga tcagccaccc attccgaaaa
     2821 atgaagggat ctttgctcga ggtttattgc aaaaaattgc cataaatgca ggggtcttca
     2881 ccatcgtaac cttgtttggt tattatttag gtagttatgt cgatactatc agcccatggg
     2941 tagacgcaag tcagcatgtc ggtcaaactg ttgcattctt gatcttagct tattcatcga
     3001 ttctgcatgt gttcaatgta cgcagcagcc aatcgatctt caaagtcaat ttagcaacca
     3061 ataaagcttt ggtagaaatg gcgttactgg cattagctat tacaacggct gttgctttgc
     3121 tgccatttac gcaagaacta ttcggcctag tacacatcag tttgaatcac tggttcttgg
     3181 tagggatttt atctatcgta ccaatcgcgg tgaatgaact gatcaagttc catcgattac
     3241 cggaagcaga agaagaggaa tgattaactg tttctaagtt gattttcttt ttactggaac
     3301 tatacaaaaa ggaggtgtga catgaatgtt gtcacacctc ctttttgcta atgggctgaa
     3361 tgtttctttt actgcctctc gataaacggt gttctcctac caacccctcg gtcacaagtc
     3421 acactctctt ttaaatatct tttggcaatt gttcataaaa tgctgctttt gttgcggaaa
     3481 tgtatgggct gagccataga taaccaatac cagcagttgc taatgctaag atatgccagc
     3541 cgataaatga aagatctaac cagaaaagtt gtcctttata accatccatc aattttcgac
     3601 ttgcagtgat cgtatccagt actttaggtg tttgtcctgt ttcttggacg atatcgtagt
     3661 agataaaata gctttgagaa taagaatagc ttttaatgat tccaggaata actaatagca
     3721 aaccccataa tgttgtaaag atcgagatca gcacagctaa gccgatcact ccgccgataa
     3781 acaaaccttt gaatccacga aatgcatctt tgaatggttc gatctgatct ttttgtccac
     3841 gtaaaatatc aagataagtc caagaaatac cgctcatgaa caaagcgcta atgatgctgg
     3901 aaataattcc gccaccgcct cctgaactac cgcctcctga agttgtcgca tctgagacgc
     3961 tcgctgatcc atcatatatg gtggtggcaa ataagataat aggaatcata ataagaagaa
     4021 tagcagcaat accaatcaat gtagggatta gatttagtaa aacagcttgt ccccaacgtc
     4081 cttttagact ctcttttgct tggcgtttta attctgcaga agattgcatg atttttccct
     4141 ccttttcttt aataacacca atataccatg tatcaaaaaa tatttctgtc ataatgtttt
     4201 tctctttttt gttat
//
LOCUS       DAFBQH010000125         4201 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.132, whole genome shotgun sequence.
ACCESSION   DAFBQH010000125 DAFBQH010000000
VERSION     DAFBQH010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.132"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(3..1223)
                     /locus_tag="LK694_002675"
     CDS             complement(3..1223)
                     /locus_tag="LK694_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBK5053488.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1243..1854)
                     /locus_tag="LK694_002676"
     CDS             complement(1243..1854)
                     /locus_tag="LK694_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBK5053489.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1897..2829)
                     /locus_tag="LK694_002677"
     CDS             complement(1897..2829)
                     /locus_tag="LK694_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBK5053490.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLSNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3254..4111)
                     /locus_tag="LK694_002678"
     CDS             complement(3254..4111)
                     /locus_tag="LK694_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5053491.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKNDTID"
BASE COUNT         1192 a          849 c          692 g         1468 t
ORIGIN      
        1 ttttatttta cagaagcact cgaaactgca tctactgcag tttcatcgtg tgtgctggcg
       61 ctggttactg catctgtttt tccaccatga acagatgcac ttgaaaccgc atcttcattt
      121 ccaacaagtt gttgacccgt ttgttgtaaa taccactcaa tgattggttt gaaatcaaga
      181 atgtattcat tgattccagc atactctcca ttttcatcat gcattgcttg gtaattatgc
      241 acaatatatt tttctggcac ttgttttggt acatgaacac gtaccgcatc tgttgctccg
      301 cttcgcagtt gttgaattac ccactctaca tttttatacg atttttctgg gtgacagttg
      361 gctaatggat tccctacctg accgggcaca cgagcagcta acatctcttt cgcttcttgg
      421 cgcaaattat aatataaaaa ttggttatta ttatctgcaa aagttaattc catcggcatc
      481 gaatctaaaa agtaattgat ttgatccact gtcaataacc cacgatttaa tttgacgtaa
      541 gtatttccag aaacggcacc gactgcttca gaagcttttt ccaaccaatg atcatctgac
      601 atatcaacat cgggtattgt cgtagcaatt gttcctgtcg catgcaaatc ttctggtgga
      661 agctttttag gccctgctaa ggtagacacg actttaatga attttacaaa acttgctaat
      721 gtactgccta aaaagtcaac agttcgtgga tcaatcaagc gatcttcttg gtcaaatgct
      781 tcttttgctt ttcccaataa aaattcattt ccgggcatca ctaatgcatt cacgcctggt
      841 gcgtccaaaa tttggcgcaa gtgtaattgc gcgcgtgaag ttccttgatc gtagtaagaa
      901 gcacccacaa ccattacagg tttattttca agtggatgga ttttaaatga tagccattca
      961 agtgcacttt tcaatcctgc tggaaccgta tgattgtgtt ctggcgtagc aataattacc
     1021 ccatcagcca ttaagatttt gcgatttagc tgttgaatag caggactatt tgtttgatca
     1081 tcatcttgat tgaacagagg taaatctttg atttcaatga tttctaaatt aaatagttca
     1141 tgatattgtt tttggataaa tgttaataat ttacggttat atgaaagttc agctgctgat
     1201 ccgacgatac ctattaattt catctttgtt tgctcccctc gattaaatcg tttcccatga
     1261 aaagctttcg gcttcacgtt tatttgcttg gtgcgcatgt tgcaattgac tagtgattgt
     1321 gacaaagagt aagaaatctt tgaatagttc acgtaatttt gcgacttgtt cttcatcaat
     1381 gagtcgtcct tgctcatcaa aggcttggag tgaatgaccc aataaaaatt ctgagcttgg
     1441 cataattcgt gcctttaact ctggtgaatc caatatttgg cgcaaatgtg cttgtgcgcg
     1501 tgaagaacct aacgtgccat aagacgctcc ggtaatcatc actggtttat ccacaaacgg
     1561 aaaaatacca tatgaaagcc aatttaatgc gttaatcaat gaagcaggaa ccgcatggtc
     1621 atactctggt gtgctgatga ttacgccatc tgcttgttca atttttccag caatttcttt
     1681 aacaacattt ggtagttctg tgttttcagg tttgttaaat agtggcagct ccttgatttc
     1741 gacaatttcg atttctgcct catttacaaa ataatttttc ataaactgta ataattcacg
     1801 gtttgttgac cgcgttgaat ttgttcctac taatccaata aatttcatct tcattctctt
     1861 tcctttctaa catcgatttc tgactgttcg cacgtactaa attttttcaa atccttttgt
     1921 atacaagaat tgattctctt ttgttacaac tgctccctcg atacccggct gttcttcaag
     1981 cgctttcaac acttctaaag aagatgctcc aaataaacgt gtagtccaga tttctccatc
     2041 tacagaagaa tcagacaaaa tcgtcaggct tgctacgtca tttttaactg gaaagccagt
     2101 tttcggatca atgatgtgat gatacactga cgaaccattt tgtagataac gttcgtagat
     2161 tccagaggta actaaagatt ggttgcttag tttaaccacc gcaagcgact cttcacgtga
     2221 acctttagga tttcgcaaac caatacgcca agcaccatcc gaacgtcttg gtgattcacc
     2281 aaaaacaacg atatttcctc ctaagttaac taaagcaaac gctgcatttc gctctcggca
     2341 atgtgtaatt aatttatctg caatgtaacc ttttgctaac gcccccaagt caatcatcat
     2401 tcctggtttg gttaaaaaaa cggttttcat tttttcatct aattgaattt ggtgtggatc
     2461 cgttagcaga agtaactcag ttatctctgc ttttgttgga accaatgcat cttcaaatcc
     2521 aatgtgccat ttttgtacaa ccggaccaat agcaatattc agataactat ttcttgcaca
     2581 actatgtgct tttcctaatt tgatgagctc aaaaagttca ggatgtacaa tcacagcttg
     2641 tcgtccagca gcttgattga tggccattaa ttcagaagtt gcatcatgtg cgctgaagcg
     2701 atgttcataa acgtgcaacc attcgcctaa ctctgctagt aatcgactag gctcttcatg
     2761 cccaacttgt aatgtaattg ttgtccccat catttgccag attttcgttt cttcgatcac
     2821 ctgattcatt ttgtaccttc tttacttgaa ctacggatta catgagtaat tagtttttat
     2881 ttttgttact gttttcacaa ccagatgata cgcctgctag atttatttgt caaccgattt
     2941 tatatatttt tttatataat aaggtaattt taatataaaa ttttatattg taaaaaaaca
     3001 aataagggaa acaaacaaca catttttgtg tgttgcttat ttccctctaa ctatcgtttt
     3061 ctatttgatg gttaacctac ttaaaataaa tactgcaacg acaaacggag atcaagcatg
     3121 ctttgtaacg tattttacga gaattacgac tttattttat tactctatct tgctgccata
     3181 cccgtctatc cctaatcatc tttggtgaat tttttcaaag ttagcataac aaaaataagt
     3241 attttttcct tcgttaatca atagtatcat tttttttcat atgatttaat tcatcaatta
     3301 attttttgtt tttattaatt ttagcaaaag ctagtgcatc ttgaatcaat tcgagcactt
     3361 ctgccttcga cttattttta gcctgtgcgt tcttcgctaa ttgggcagca gctcgtgcta
     3421 agtaataagt caaatggtta tccgaacaaa tatcgattgc gtaatttagt agtgcatcac
     3481 ttgtttccaa atcaccgtct tgcgcaaaaa aaacaccaca atgaaagacg atatttaaca
     3541 cccgccagat atcttctgtt gtacgaatcg gatacgtata aattttttca aacactcgat
     3601 tgaaataaac ttctgctttt tccaactctt tttctctagc atagaccatt ccaatacctg
     3661 tatatgccaa taaacgaaac aacgtgttgg ctgtttctgc catcaacaac tgatcaaaat
     3721 ggaacaacac atccgtaatt ggtgcattcg taaaaatcat ttcaaatcct tttagatagt
     3781 agtaacgtgc aatcagctca gactcgtcaa ttaccttggg atcaatggct gctaaaatct
     3841 gtgtcaattg ttcatattca ctcgtaatca gtgaaaactc tgctttttct agctgttcat
     3901 caagttgtgt attgatcacg ttcatcgaag aaaaaagttc aactaacgga agattgattc
     3961 gattacataa tttgattaaa atttttaacg aagggacttg accattgttc tcaaatttac
     4021 ttaatgttgc ttgtgtacaa attccttcag acacttcttt ttgagataag ttggcagcct
     4081 ttcttgccgt aataaatttt tcaatgttca ttattttctc tccaatcaga cctcacacga
     4141 aaatcatttc tgtctcgatt ttagcatgtt ttctttggga atgctttaat tggttaagat
     4201 a
//
LOCUS       DAFBQH010000126         4156 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.133, whole genome shotgun sequence.
ACCESSION   DAFBQH010000126 DAFBQH010000000
VERSION     DAFBQH010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.133"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            178..774
                     /locus_tag="LK694_002679"
     CDS             178..774
                     /locus_tag="LK694_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBK5053492.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            898..2697
                     /locus_tag="LK694_002680"
     CDS             898..2697
                     /locus_tag="LK694_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBK5053493.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2975..3187)
                     /locus_tag="LK694_002681"
     CDS             complement(2975..3187)
                     /locus_tag="LK694_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053494.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3649..3798
                     /locus_tag="LK694_002682"
     CDS             3649..3798
                     /locus_tag="LK694_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053495.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            complement(4051..>4156)
                     /locus_tag="LK694_002683"
     CDS             complement(4051..>4156)
                     /locus_tag="LK694_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK5053496.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1228 a          706 c          729 g         1493 t
ORIGIN      
        1 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
       61 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
      121 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
      181 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
      241 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
      301 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
      361 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
      421 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
      481 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
      541 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
      601 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
      661 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
      721 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
      781 ttttgtaaaa ttagtcattg attgacaatg ttaaatctgg gaaagggtct gcaaaacaca
      841 ctgcaccctt tcctttttca ttagatttgc gctacaatgt gaagagcagg tgataggatg
      901 aattatttga aacgtagctt aggtaatgct tgggactttt taaaaggaac acaagcttat
      961 ttccgtgatg tcttgctgat gcatggcttt attttattta tttgtttacc cttattgagc
     1021 agtacgaccc gttttatttt acaccgaggg gcaattgact atttgtctta tgacaatatt
     1081 ccttcaattt tttctcaaca tcctggggtt ctactctcgt taatcgtagt tttgatgacg
     1141 attttactgt tagtttattt cgaatttact tttcttttga tgagtgtttt ttttattaaa
     1201 aagaaagaac caatctcttt gaagcaactc ttacacttga cgatacttca gctgaagaaa
     1261 gtgagaccca ttactttttt gtttttcctt gcttattttt tattgattct accgatcagc
     1321 ggattaagtt ttaattctga tctactgtca aaaataaaaa tccccgcttt cattatggat
     1381 tttatttttg ctaatcgttg gatcatcgtc agttcctttt tactcgttta tattttcctt
     1441 ggctatattg ggattcgact gatttttgct ttaccggaaa tgattctacg ggatcgtcct
     1501 tttcgagcag ccattcggga aagctggtca ttgaccaaat cgcgtctttt ggccatcacg
     1561 ggccaattta ttgtgattgg cggtacgatt ttactccttt cttctttagg atatatcgtt
     1621 gttattttgg cacaaagtat ggttgaacaa tttttcccag actatgcatt gatcagcgct
     1681 gtgtttgcta tgacgctttt acaagggatt ttattattca atatcgtgat gtctaccgta
     1741 gggatttttt atatcattgt tgatttcatg gatgacgaag gcttcttgcc tgagatccct
     1801 aagtggttta tcccacaagc ccccaacttg cgtttctctg cgctgaaaaa cactggtttg
     1861 actctttttg cagttttttt cggtattggc gtctgtcttt acaatatgga ttatctgact
     1921 tcagctgttc agacaaaacc agtgactgtc tctcacagag gtgtgagtac ccaaaatggg
     1981 gcgcaaaata ctttagaagc acttgaaaaa acaagtagag actatcatcc tgattatgta
     2041 gagatggatg ttcaggaaac aaaagatggt catttcgtag tgatgcatga tgcaaatctt
     2101 cgtcacttaa caggggtaaa tggaacacca caggatctta cattgaaaga attgacaaat
     2161 cttacagtta ctgaaaacgg agtgcaagcc cctgtttgtt cttttgattc ttatttggaa
     2221 aaagcacata agctaaacca aaaattactt attgagatca agacctctaa aaaagattcg
     2281 gatgaccttg tcgaacgatt tgtccataaa taccgcaaag ttatcttaga gaaccatcat
     2341 attttgcaaa gtttgacgta ccaaaccgtt tcagatttaa aagaagcaga accaaacttt
     2401 tatgtgggct acatcttacc tttcaatatt gttgggcccc ctgtgacgcc tgctgatttt
     2461 ctgacaatgg aatatagtac gatcaaccgt aacttcatta attctgccca ccaagacgga
     2521 aaagaagtat atgtatggac aatcaacgat tcagatgcta tgagtcgcat gatgttttat
     2581 ggtgtggatg gtatcataac cgatgagatg ggactactaa atgaaagtgt gaaacaaaca
     2641 gatgaagaaa tcacttattc agataaactg ctaaattttg ttttgggagt cggctaatca
     2701 aaaatcgtta atgaaaaaag tctttataca acaaaaaacg cataacctgc caccgcaggt
     2761 atatgcgttt tttgtttgac gctcataatg tgtgcttaac tttctcttga ttccttcatc
     2821 gcacgaacaa tagctaaaat agcaggaatt gcagagatca ctgcaagaat aattcccatt
     2881 ttccctgaac tttttctttt tggcatatct tctcgctccc ttctgtcttt atttatatta
     2941 taagggagtt cttgcgaatt ctcaaaaata ctgtttaaga aaccaattca gcaggtgcgc
     3001 taatatcaca gaattttacg agcatgcgat acttgctttc attgataagt tgatagtctc
     3061 gttggtcata actttcaaca tacacgacag ccgtgttgat atatgtacgt tcaatacagc
     3121 cgattagttc atgagatact ccgatcggtt tgcatatata agatcttcct acttctgctt
     3181 ttaccatttc attccctctt ttctttattt ttattttgtt ttttctttat tttataataa
     3241 caaattacct tgtatttgaa aacgttttta tccatttttt tattttttat tttgagtatc
     3301 tttctgcttt ttagataact attttttaac acgatcttag acgttgtgac aaaaacgttt
     3361 gtttatattc ctatgttgtt cgtgcgtttt tttctttata ttgctaagtt atttataaag
     3421 aggtgagcat atgatttata ttcaagcaat agtatttgta accttgatga ttgggctact
     3481 tatcggatta aaagaaaata gctaaaccat ttaagaaaaa ataaataagg aaaatgataa
     3541 atgcttttta attgcatcaa aaaacgtacg atgctaaact tggtataagt tcgaaagttt
     3601 aaaggaaact ttcaagaact aaattacatt acaaaggaga tgaattttat gacgtttaca
     3661 aaaacaaata tgcaataccg cgtagctttt gatacaactt ctaaccgttt tatggcgatt
     3721 gatgcacaaa acgaaaataa cgttgcgttt ggtattacaa tcgaacaagc agtccgagca
     3781 ttaaacgaat ctagataagg agtgaaccat tccaaatggc gactgatgag atctcggaaa
     3841 atcaataaaa gacgtttgta acggtaatcc gcgacaaacg tctttttgta ttcattacat
     3901 atatttggaa attgtctctc tttatctcta gacctaaaca aaaacacaag ttctaatatc
     3961 aaccatctcg ttttttatga caattttgtt agctgtttcg atgataatat tccgttatta
     4021 tctgtataat atttaatata atcttaattg tcaaattaag ttagacagat catcaatact
     4081 tacgtaactc aaaaatactt ccaaaggtgt tcgataatcc agtgattttc taggaatatt
     4141 atttcgttta gatgcg
//
LOCUS       DAFBQH010000127         4082 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.134, whole genome shotgun sequence.
ACCESSION   DAFBQH010000127 DAFBQH010000000
VERSION     DAFBQH010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.134"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..536
                     /locus_tag="LK694_002684"
     CDS             <1..536
                     /locus_tag="LK694_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HBK5053497.1"
                     /translation="STSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDNLKLDFQ
                     QAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKVMTKGVT
                     NIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYAVPYVFF
                     AIILWLIALFFRVFNDL"
     gene            1212..1466
                     /locus_tag="LK694_002685"
                     /pseudo
     CDS             1212..1466
                     /locus_tag="LK694_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1536..2048
                     /locus_tag="LK694_002686"
     CDS             1536..2048
                     /locus_tag="LK694_002686"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5053498.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            2045..3082
                     /locus_tag="LK694_002687"
     CDS             2045..3082
                     /locus_tag="LK694_002687"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK5053499.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
     gene            3242..3418
                     /locus_tag="LK694_002688"
                     /pseudo
     CDS             3242..3418
                     /locus_tag="LK694_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            3473..4082
                     /locus_tag="LK694_002689"
                     /pseudo
     CDS             3473..4082
                     /locus_tag="LK694_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1522 a          646 c          790 g         1124 t
ORIGIN      
        1 cttcgacctc gaactttaat aataagttag atgtttttga agaaaagact aagcatgcaa
       61 gattaaactt tgaaacaact gcaaagcact atattaaacg acttgatgaa gataatttga
      121 aattagattt tcaacaagct attcaagatg aattaagcga tactaaagac gaaatacgtg
      181 aagtgacgaa acaagcgaga gaagaaacaa aagagtataa agaaatctta gaaagtaaaa
      241 taaaagacca taacaaagtg gttgataaat cgaatacagc gctaaaagtg atgacaaaag
      301 gcgttacaaa tatatttttt gtgctaatta tttttgtact tgtaatgctt gtgacaggtc
      361 cgataggtca tttctttggt attgaacatt tatatagctt tattaatggt tttattgatg
      421 accacgaaag tgcatggcga tatttgatgt tgatacttta tgcagtaccc tatgtattct
      481 tcgctatcat tctgtggctc atagcattgt tttttagagt atttaatgac ctttaggtag
      541 atttatatat aaaaagggct aaatacgcaa aataccccct taaaatgcaa aataaatgca
      601 aataaggggg taactcctta tgaaaaaaca actaatagta ttataacacg tttttatgcc
      661 gagaaaactt attggttgga atgggctatg tgttagctaa cttgttagcg agttggttgg
      721 acttgaattg ggattaatcc caagaaagta ccaactcaac aacacataaa gccctgtagg
      781 ttccgaccaa taaggaaatt ggaataaagc aacgaaagga gttgagtaaa atgagtgaga
      841 aaacgaagaa aatcattcga gtatcattag ctttttcaca agtagtagtg caatcattga
      901 tagtttatta tacttaccag caatataaat tatctaaaga gaattaagtt ttgattatgt
      961 aataagtttt ggtactaaaa aagacttgat ctaattagac caagtctttt gttagtgtta
     1021 tattaataac aaaataaaaa taagaagtcg ctcactcccc gaccaaagtt tgtgagcaaa
     1081 ataaacagtc gatgtttgtt tcttaattgt atatcttgat attaaacgat ttaagattat
     1141 tcttcaagat atatattcgc aaatgtaatt ggaaaagcga cttcttaaac aaaattaagg
     1201 agtcgctttt tatggcaaaa ataacgaaaa atgagcaaga aaatttggtg gaaaattaca
     1261 caaaaaaacc ggcgtactct aatagccggt tagacgcaca tttcgtgtgc acttctaatc
     1321 cgaaattgag ctttgacgct atgacgatag tcggaaacct taataaaaac agcgctaaaa
     1381 agctatccga ttttatgagt ttagatccac aaattagact ttgggatata cttcaaacaa
     1441 aatttaaagc taaagcacta caagaccccc tatgttaggc tagttgtcgt ataggataat
     1501 aagttataat aaagactata cggaggatga ttaccatgac aaataaaaac aatgaacgtt
     1561 actctaccga actgaaagcg acagccatca agaaaatgat gccacccgaa aataggggga
     1621 ttcgccaatt aagtcaaact ttgggcattc cagaagcgac tctttgttct tggcgtaaaa
     1681 aagctagaat tcaagggaat actacacccg gaacgaaaaa aaatgtcgaa aaatggtcaa
     1741 gtgaagataa atttttaatt gtgatggaat cattctcaat gaaccaagta gaacttgcgg
     1801 aatattgccg aaagaagggc ttatttaaag aacaaattga ggcttggaag aaaacgtgct
     1861 tatctgcgaa tgaacaggaa gaaaatcgaa cgagagaact tgcaactgaa ctaaaagaag
     1921 agaaaaaaca agcgcgtcaa ttagagaaag aattacgtca taaggaaaaa gcattagcgg
     1981 aagccgctgc tctcttatta ctaagaaaaa aagcacacgc gatttggggg gaccaagagg
     2041 aagaatgatt tcatcttcag atcgcaaaca agctgttgaa cttattcaag aagccaatcg
     2101 caatggggcc cgtctcacct atgcctgtaa tgagctgaat attagcgttc gaacctacga
     2161 acgatggacg cgtgaaggta cgatagcgca tgatcaacgc cctttggcga aacgtcctgt
     2221 acccaaaaat aaactgacgg atcaagaacg cgaaaaaata atagaaaccg tttcaaaaaa
     2281 agaatttatg aatttgccac ctagccaaat tgtaccaaaa cttgcggatt gctcgattta
     2341 tattgcatca gagtctagct tctatcgtgt attgcgagaa aaaaatatgc aacatcaccg
     2401 tggccgaagt caagtttcac aaaaacgcat accgccaagt caccttgcga cgaagccaaa
     2461 tgaagtttgg acttgggata ttacttggct aaaaggtcca ataaaaggac tcttttaccg
     2521 gttatatctc atcattgatt tatttagccg taaaatggta ggctgggaga tttgggaatc
     2581 agaagaagct cgtcatgccg aaaaacttat taaacagacg gtgcttaagg aacgtatcca
     2641 aggacagccg ttggttttac actccgataa tggcagccct atgaaagccg cgacttttca
     2701 agtcttactc gaaaaattag gaattcaaag ttcttattca agaccacgag tgagtaatga
     2761 caatccttat tcggaagcgg ctttccgaac gttaaaatat cgaccagaat atcccaataa
     2821 cggctttaaa acaatagatg atgctcgaaa atggacgaaa gagtttgtcg attggtataa
     2881 cgattctcat ttgcatagtg ggattcaatt tgtgtctcct gcagcgtgtc atgaaggacg
     2941 acatctcgct attttagaga aacgaaagca ggtttacgag gacgctaaaa agcgccatcc
     3001 tgaacgctgg tcaaaaggtg ttcgaaattg gactccacat caatcggtgg cgttgaatcc
     3061 tttaaaagaa acagaaagtt aacaggagca taatctttca agattatcaa ataaccctcg
     3121 tcacccaata ggggaaaaag ttattcttcc cctattggat aacgaggcca accgaccata
     3181 gggaggttct gctttttgat aggtggaaaa taaaaatacg acaactatat tgacaaacac
     3241 cgaccatcgt ccagtaaaga gacgcaataa attctatcga agtttacgca ctgcctctac
     3301 aacgattaaa ggcatggaag ccatccgagg attatataag aaaacccgaa aagaaggcac
     3361 tctcttcggg ttttcggtct gtactgaaat caaggtatta ttgggaatcc cagcttaaat
     3421 catagatacc gtaagggatt ttattcttta tttaaaactt tgcaacagaa ccgaatcatt
     3481 aaaaaaacat tatacagagc cttgggaaga tgaagttttt agagtaatta ttgaatataa
     3541 caatgttcct attggatatg gacaaatata taaaatgtat gatgagttat atactgatta
     3601 tcattatcca aaaactgatg agatagtcta tggtatggat caatttatag gagagccaaa
     3661 ttattggagt aaaggaattg gtacaagata tattaaattg atttttgaat ttttgaaaaa
     3721 agaaagaaat gctaatgcag ttattttaga ccctcataaa aataatccaa gagcaataag
     3781 ggcataccaa aaatctggtt ttagaattat tgaagatttg ccagaacatg aattacacga
     3841 gggcaaaaaa gaagattgtt atttaatgga atatagatat gatgataatg ccacaaatgt
     3901 taaggcaatg aaatatttaa ttgagcatta ctttgataat ttcaaagtag atagtattga
     3961 aataatcggt agtggttatg atagtgtggc atatttagtt aataatgaat acatttttaa
     4021 aacaaaattt agtactaata agaaaaaagg ttatgcaaaa gaaaaagcaa tatataattt
     4081 tt
//
LOCUS       DAFBQH010000128         3956 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.135, whole genome shotgun sequence.
ACCESSION   DAFBQH010000128 DAFBQH010000000
VERSION     DAFBQH010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.135"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..156
                     /locus_tag="LK694_002690"
     CDS             <1..156
                     /locus_tag="LK694_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBK5053500.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="LK694_002691"
     CDS             175..1452
                     /locus_tag="LK694_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5053501.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="LK694_002692"
     CDS             1462..2118
                     /locus_tag="LK694_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK5053502.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="LK694_002693"
     CDS             2118..3494
                     /locus_tag="LK694_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK5053503.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3624..>3956
                     /locus_tag="LK694_002694"
     CDS             3624..>3956
                     /locus_tag="LK694_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBK5053504.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1594 a          423 c          693 g         1246 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc gacatc
//
LOCUS       DAFBQH010000129         3902 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.136, whole genome shotgun sequence.
ACCESSION   DAFBQH010000129 DAFBQH010000000
VERSION     DAFBQH010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.136"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..189
                     /locus_tag="LK694_002695"
     CDS             <1..189
                     /locus_tag="LK694_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK5053505.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(275..1369)
                     /locus_tag="LK694_002696"
     CDS             complement(275..1369)
                     /locus_tag="LK694_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBK5053506.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1558..2319
                     /locus_tag="LK694_002697"
     CDS             1558..2319
                     /locus_tag="LK694_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK5053507.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="LK694_002698"
     CDS             complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="LK694_002698"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBK5053508.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3094..3849
                     /gene="fabI"
                     /locus_tag="LK694_002699"
     CDS             3094..3849
                     /gene="fabI"
                     /locus_tag="LK694_002699"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBK5053509.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         1261 a          755 c          684 g         1202 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgtaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcataac ctataaaaaa acaaaataaa aaagcagaaa taactggcta
      241 tttctagcgg attatttctg caataactaa ttatctattt tttattttca attaatttaa
      301 agcttgacca tggtttcaaa aagtcaagga ggaataattc gtcatcggag attcttccta
      361 caacattgac acgtccgtcg tttttcatat ccttcaaggc gatttgtgtt tctcctttgt
      421 attgaccgta cccttcattg tcaatcagta catccccatg atgcatatct cgcgtattat
      481 gaggcgggaa atctttatct ttgtagtata cacgagtcat cgttgaacga aggatatatt
      541 ctgagcgatc ccctcgataa ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg
      601 taataccttc ttccgtatct actttcaatg ttggataatc cgcgttaaat gcttctgcca
      661 tttctttcaa ttcttcttca cttgcgtacg catttccaat agaaatatca tcaatcaatc
      721 ctgtaaggat taaatgcttc acttgtgtcg caatttcaag atctcggtgg tcttccagcg
      781 tgcacaagcc atcttgcgta ggccaagggc caaactcagc agattgcgaa ttgacgaatg
      841 ccatcgtatt caaattatat tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc
      901 ctaaaccaga gtatcgatga ggataaaaat tatgagaacc taataaatta tccgtgtttg
      961 gcgaataact catgatgttg tcaacgtaat tcgtgcccga actcatattg atctcgattt
     1021 tgataccaaa aggattgcgc gtcatttttg cttcttctgc tcctgtgaat ccgatatcta
     1081 agcggacccc gtaggcaccc atcttatgga aaaaagataa gtcatcgtaa gaaatctcca
     1141 actgttcaaa taacgctgga ttgatatcaa ccattacttc catgcctagg ctattcgcgt
     1201 aatcaactac ttctttaaat tctgaaagaa ctttttcttt atcatcattg atctgcagca
     1261 aactggtaaa aacccgttta tacccatact tatgagctaa atccaagtac gctttgtcct
     1321 tttcaaatgt tgaccgttct ggataaatcg agattcctaa ttttcccata ttaccatttc
     1381 ctcctgcatc ttttcgtttt tgtatccact tacattatag caaacacaaa gtaaatggtc
     1441 tacacctttt ctttttcttt taaaataaat gataaattcc aataatttaa gtaggtattt
     1501 tccctctatt ttgttatcat taaagataac aaaatagtaa attggaaagg aacagttatg
     1561 aagaaaaata aagttatttt aagtattagt ttagcagcag cattgctgtt tgctgctggt
     1621 tgttctgcaa acaacacgac ttcttctagt tcgtctacag aaagttcaaa aagtacatct
     1681 actgcggaaa gcacgactgc cagtagcgtc gatttgaacg cattggaatt accacaattg
     1741 acgaaagatg tcgccgaaaa tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc
     1801 aaaatcaaat tatttcccga acacgcacca aaagcggttg aaaactttat gacacatgca
     1861 aaagacggtt actacaacgg actgactttc catcgcgtca tcaaagattt catgatccaa
     1921 ggtggcgatc ctaaaggcga tggcactgga ggggaaagca tttggggaga aggatttgaa
     1981 acagagatca acaaccacct ttataatata agaggtgcgt tatctatggc tcgctcacaa
     2041 gatcctaaca gtaacggcag ccagttcttt atcgttcaaa atacagatga tatgcatgat
     2101 ggtcttttaa aagacgacta tcctcaagca atcatcgacg cttataaaaa cggcggttat
     2161 ccttcactag atggccatta tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg
     2221 gatgctattg caaatatcga aacagatagt tcagacaaac caaaagaaga agtaaaaatc
     2281 gataaaatca atatactgca agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg
     2341 catagatgac aacgtatgct aggattcagg cgtcttcttt ttacataaaa agcaagtaag
     2401 cttccttttg ttttgctata tcaggtttta tagtcaagca ttttggttga cgatgaacgt
     2461 gaactcgcac tcacagactt ttttatcatc tacatatgct gcagataacg ctgtaccgac
     2521 tgatcccttc attttggtga tttcaaaatg acatttcagc acatctcctg gaacaacttt
     2581 ttgacggaat ttggccttat tgatcccacc gatataagct ctttttccta gaaactcttc
     2641 tgattttagg ataagtatcg aacctgcttg tgccagtgtt tctagaatca acgcacccgg
     2701 catcactgga ttgtcaggaa agtgaccttg gaaaaaatct tcattgattg ttacattttt
     2761 tgtcgcaacg atcttttttc ctggttctaa agtatctaca taatcaatat aacaaatagg
     2821 ataacgatta ggaatcaatt ccattacttc agtagcagat aatacgtaag acataattaa
     2881 actccttctt tcaggcaatt gccattttca ttactttgac tatcaaagta tcatattcct
     2941 aaatatttag caatcttttt ttaatttaag aaaaaatagt ttgacaatca aaacattttg
     3001 actaataatt aagaaatcaa gaagttattt tagctattat tctctatttt tttacgacta
     3061 tcaaattttt atcagaaaca aaggagagtc attatgtcat ttctagaagg aaaaaaaatc
     3121 gtcattatgg gcgtcgctaa caaaaaaagc attgcttggg gttgtgctga agcaatgttg
     3181 aaacaagggg cagaaattat ctatacttac caaaatgaac gaatgaaaaa atcattagtc
     3241 aaattggtcg gcgaagaagc attcactgta gaatgtgatg tttctagcga cgaaagcatc
     3301 gaaaaagcaa tgaatgccat cggtgaatat gctggggaga tccacggcct tgtccatgca
     3361 gttgcttacg ccaacaaaga agaattgtcc ggaaatgtgt ctgatatttc cagagacggc
     3421 tatcaattag ctcaagatat cagcagctac tctttaattg ctgtatcaaa atacgcacaa
     3481 ccgtttcttg caccaaacag cgggatcgtt agcatgactt atctaggttc agaacgcgca
     3541 atcccgaatt ataacatgat gggtattgcg aaagcttctc ttgaagctgc tatccgttat
     3601 ttggcagctg agttctcgcc taaaggaatt cgggtcaatg gaatctcagc tggggcaatc
     3661 aaaacattag ctgtcacagg tgttaaagac tatcaaaaac tgatccagtt atctgaatct
     3721 cgtacgcctg atcagcaagg tgtgacgatc gaagaagtcg gtaatgcttg tgctttctta
     3781 atcagcccac tttccagcgg aatcatcggt gatattatct acgttgacaa aggtgtccat
     3841 ctttcataaa cttttgttgc attctttaaa gtaaaacata taaaaatcag gaaaaaactt
     3901 tt
//
LOCUS       DAFBQH010000130         3883 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.137, whole genome shotgun sequence.
ACCESSION   DAFBQH010000130 DAFBQH010000000
VERSION     DAFBQH010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.137"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            91..3123
                     /locus_tag="LK694_002700"
     CDS             91..3123
                     /locus_tag="LK694_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBK5053510.1"
                     /translation="MVENKEIRFEQDIETSLTTYGKWKKETFNESNFDPIIGLDCQKL
                     LGFVQKTQPKQWARHQRNYNNDGEVKFLQRFNQEVETHGMLHVLRNGIKDRGVRFKVA
                     YFKPASELNDETMEKYEANSFTCTRQFAYSPNNHKTIDMVLSLNGIPLVALELKNQYT
                     GQTVENAKLQFQKDRDPKELVFRFNRRFLVYFAVDHTDVYMTTKLDGKKTYFLPFNQG
                     SNGPGRVGGAGNPSNKDGYPTAYLWEKVLTRDNLMDIIQRFMNLEIKKEKQGDREVTK
                     KTLIFPRYHQLDVVRKLVADVKSHGSGENYLIQHSAGSGKSNSIAWLAYHLASIHDQL
                     NYPIFSSIIIVTDRTVLDRQLQNTIMNFDHQTGLVETIDDRKSSQDLKEAINDGRKII
                     ITTLQKFPVIYQEVDQVEGRNFAVIVDEAHSSQTGNSAQKLKAALSDKSKSLEEYYEL
                     EGEVDDVLPDAEDELVQQLVAQGKHQNLSFFAFTATPKEKTLEMFGTKQPDGHFEPFH
                     VYSMRQAIEEGFILDVLKNYMTYSTSYKIAREIEDNPELPKSEATRAIVRFQSLHPWA
                     VTQKTAVMVEQFRAVTRTAIGGQAKAMVVTASRLHAVRYMKEFKKYIANKGYNDLDVL
                     VAFSGSVVDGETEYTETKMNVTKSGENVRENQLKEAFHTDNFNILIVAEKYQTGFDEP
                     LLHTMFVDKKLKGVKAVQTLSRLNRTMPGKKDTFILDFVNDVEDIEAAFQPFYEQTNL
                     REEINVNLIYDTQSKLRKFNVYNQGDIERVMKLVKQAQKQQNERLLGRISSMFKPIMD
                     RYEEMNKDNQYQFRVTLRNFGKWYDYISQLDRLFDMELLEERIFTGYLLKFLPKETRE
                     NIDISDKVKLEYYKLRQDFSGEIHLVNKEGELVNPKSIDAGIKPPDERDSLEEIIHKI
                     NERFPNDFDEGDRVLVEILYRSFADNPDLKVVSMAKNNDAQMFEKSLFPDVFKDKVME
                     QYEKNNAAFEKMFGHDDKYFDLVYTLVARDLYKLLRTK"
     gene            3313..>3883
                     /locus_tag="LK694_002701"
     CDS             3313..>3883
                     /locus_tag="LK694_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBK5053511.1"
                     /translation="MSTTIEVNKQSVKQFLETGKIKKFVIPEYQRPYAWTDEQNQVLF
                     DDLAEYTANNNESTYFLGSIVAYENDHNEQEIIDGQQRITTLFLFLRAIYAKLENSCE
                     KEALFLKSQIEPALWEQDDLTGEVKPDKILIMSRVMGDEGNEEFASILVSGEADVKSK
                     SNYSKNYILIQHLLNEYATNEPLSFYRFIS"
BASE COUNT         1329 a          621 c          824 g         1109 t
ORIGIN      
        1 atagctgaat tgatggaact tgagagttat aaaaaagctc tcatctacga atacgtaacc
       61 ggtaaaaaag aagcctagga ggtgattcat ttggtagaga acaaagaaat tcgttttgag
      121 caggatattg agacttcact gactacgtat ggcaaatgga agaaagaaac attcaatgaa
      181 tcaaactttg atcccataat tggtttggat tgtcaaaaat tgctaggctt tgtccagaag
      241 actcaaccaa aacagtgggc acgtcaccaa cgaaactata acaatgatgg agaagtcaag
      301 ttccttcaac gctttaatca agaggtagaa acccacggca tgttacatgt attgcgcaat
      361 gggatcaaag accgcggtgt gcgctttaaa gtagcttact tcaaacctgc ttcagagctg
      421 aatgatgaaa cgatggaaaa gtatgaagca aatagtttta cctgtactcg ccagtttgct
      481 tacagtccta ataaccataa aaccattgat atggttttgt cgttaaatgg tattccgctc
      541 gttgctttag aattgaaaaa ccagtacact ggtcaaacag tagaaaatgc caaactccag
      601 tttcaaaaag atcgtgatcc aaaagaattg gtatttcgtt ttaatcgccg cttcttagtc
      661 tattttgcgg tggaccatac agatgtttat atgacgacaa agttggacgg taaaaaaacg
      721 tacttcttgc cattcaatca aggttccaat gggccgggaa gagttggtgg agcaggcaat
      781 ccgtccaata aagatggcta cccaactgcc tacttgtggg aaaaggtgct tactagagac
      841 aatttgatgg atattatcca acgttttatg aatctagaga ttaaaaaaga aaagcaaggc
      901 gacagagaag tcacaaagaa aacgttgatt tttcctcgtt atcatcagtt agatgtggtt
      961 cgaaaattgg ttgcagatgt aaaaagccat ggtagtggag aaaattattt gatccagcat
     1021 agtgctggtt ctggaaaatc taatagtatt gcatggctgg cataccattt agcctctatt
     1081 catgatcaac tgaactaccc aattttttca tcaattatta ttgtgactga tcgaacagtg
     1141 ttggatcgcc agttgcaaaa tactatcatg aactttgatc atcagacagg tttggtagaa
     1201 acgattgacg atcgcaaatc ctctcaagat ttaaaagaag ccattaatga tgggcgtaag
     1261 atcattatta ccactttgca aaagttccca gtgatctatc aggaagtaga tcaagtggaa
     1321 ggtcgtaatt ttgctgtgat tgtggatgag gcacattctt cacaaactgg gaacagtgcc
     1381 cagaagctaa aagccgctct ttcggacaaa tctaaatcct tggaagagta ttatgaactg
     1441 gaaggcgaag ttgacgatgt attaccagat gctgaagatg aactagttca acaattggta
     1501 gcacaaggta aacatcaaaa tttaagtttc tttgctttta ccgctacacc gaaagaaaag
     1561 acattggaaa tgtttggtac gaaacaacca gatgggcatt tcgaaccctt tcatgtttac
     1621 tcgatgcgcc aggcgattga ggaaggcttt atcttagatg tgctgaagaa ctatatgacc
     1681 tatagtactt cctacaaaat tgcccgtgaa attgaagata acccagaatt accaaagagt
     1741 gaagcaaccc gagcgattgt tcgtttccaa tcattgcatc cttgggcagt tactcaaaag
     1801 acggctgtga tggttgagca attccgagcg gttactcgta cagctattgg tgggcaggca
     1861 aaagcaatgg tcgttaccgc atcacgctta cacgctgttc gctatatgaa agaatttaaa
     1921 aaatatatag cgaacaaagg ttataatgac ttggatgtgt tggttgcttt ttctggttcg
     1981 gtagtagatg gagagactga atatacagaa acgaagatga atgtcacaaa atctggtgag
     2041 aatgttcggg agaatcaatt aaaggaagct ttccatacag ataattttaa tatcttgatt
     2101 gttgctgaaa aatatcaaac gggttttgat gaaccgttgc tgcataccat gtttgtcgat
     2161 aagaagttaa agggtgttaa agctgttcag actttatcac gactcaatcg aacaatgcct
     2221 gggaagaagg atacctttat tcttgatttt gtaaatgatg ttgaagatat tgaagctgct
     2281 ttccaaccct tttatgaaca aacaaatttg agagaagaaa tcaatgttaa cttgatttat
     2341 gatactcaaa gcaagctacg aaaatttaat gtttacaatc aaggtgatat tgaacgtgtc
     2401 atgaaattgg tgaaacaagc ccaaaagcaa cagaatgagc gcttgcttgg acgaatttct
     2461 agcatgttta agccgattat ggatcgctat gaggaaatga acaaggataa tcagtaccag
     2521 tttcgggtga ctctacgtaa ctttggcaag tggtatgatt atatttcaca acttgatcgg
     2581 ttattcgata tggaactatt ggaggaacga atttttactg gatacttgct gaagtttcta
     2641 cctaaagaaa cacgagaaaa tattgatatt tctgacaaag taaagttgga atattataag
     2701 ttacgtcagg acttttccgg tgaaatccat ctagtgaata aagaaggcga gctagtaaat
     2761 ccaaagtcaa ttgatgcagg aatcaaaccg ccggatgaac gagattcact agaagaaatc
     2821 attcataaaa tcaatgaacg atttccaaat gattttgacg aaggagaccg ggtgttagta
     2881 gagattttgt atcggtcatt tgccgataac ccagatttga aggttgtcag catggctaag
     2941 aataacgatg ctcagatgtt tgaaaaatcg ctgttccctg atgtatttaa agataaggtg
     3001 atggagcagt acgagaagaa caatgcagct tttgagaaaa tgtttgggca tgatgataag
     3061 tactttgatc tagtatatac attagttgct agagatttat acaagttatt acggacgaaa
     3121 taaaatttat aaataaggat tagacaatac aatttctctc aaaaataaag gagaagttgt
     3181 attgtttgct tttaagaaag ccctgaaaaa tttgttacgt actaaattgt ctgaactctg
     3241 tcatatcagc tatgtcagaa taaaggaaat agatttattt ttataaacag tgtcgtctcg
     3301 gagatttaaa ctatgtcaac gacaattgaa gtgaacaaac aaagcgtgaa gcagtttctt
     3361 gaaacaggga aaatcaaaaa attcgttatt ccagaatatc aacgtcctta tgcctggact
     3421 gacgagcaaa accaagtact atttgatgat ttagcggagt acacagcaaa caataacgag
     3481 agtacttact ttttaggaag cattgtggct tatgaaaatg atcataacga gcaagaaatc
     3541 attgatggac agcaaaggat aactacgcta tttttatttt tgagagctat ctatgctaaa
     3601 ttagaaaata gttgtgaaaa ggaagcttta ttcttgaaaa gccaaattga acccgcgtta
     3661 tgggaacaag atgatttaac aggtgaggtt aaaccagata aaattctgat tatgtctcga
     3721 gtaatggggg atgaggggaa tgaagaattt gctagtattc tagtaagtgg tgaagcagat
     3781 gttaaatcaa aaagtaatta ttcgaagaac tatattttaa ttcaacatct attaaatgag
     3841 tatgcaacaa acgaaccact atcattttat cgttttatca gta
//
LOCUS       DAFBQH010000131         3865 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.138, whole genome shotgun sequence.
ACCESSION   DAFBQH010000131 DAFBQH010000000
VERSION     DAFBQH010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.138"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            629..946
                     /locus_tag="LK694_002702"
     CDS             629..946
                     /locus_tag="LK694_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBK5053512.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1037..1402)
                     /locus_tag="LK694_002703"
     CDS             complement(1037..1402)
                     /locus_tag="LK694_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053513.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1610..2092
                     /locus_tag="LK694_002704"
     CDS             1610..2092
                     /locus_tag="LK694_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053514.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2289..3179
                     /locus_tag="LK694_002705"
     CDS             2289..3179
                     /locus_tag="LK694_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HBK5053515.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(3466..>3865)
                     /locus_tag="LK694_002706"
     CDS             complement(3466..>3865)
                     /locus_tag="LK694_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5053516.1"
                     /translation="EPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYY
                     FVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNNKI
                     KLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         1303 a          609 c          720 g         1233 t
ORIGIN      
        1 cttattgctt ttgcaaaatt tatttacctg ttttctggat caacgatagt gtacattatg
       61 ctatccttgt ttggatttta ggacgtttaa agaggcactt ttttacataa cttaatccat
      121 agacctttta agcatgctgt gagataataa taaagaagag tttaaagcgc accccaaacc
      181 acttccccat aagtgtatta cgctttaaac tcttttatat ttgaattcac taagaagcat
      241 accatatatt tgaatttact aagaagcata ccatattttt gaaaaaaagt gagaaaaaag
      301 gcttataatt aggatttaga gtaattaatt agatgtttat tttataggac tgatactata
      361 gattacaaat agtatcgtat ttcgcaatct taaaattatc ttcttactgt gaataagata
      421 ataaaatttg tatttgtcct ttttaggtaa cttattttaa tctatatacc tttctggatc
      481 aacgaaagta tactttatat agtcataacg ccgatggtaa ctatgcactg taacgataat
      541 gctgctaaaa tatcttttta cgaaaaacat cacaaggaaa aacttaacta tggtattatc
      601 taaacataat tttttaggag gtaatatttt gcttttttat agtaatttta tcttaatagt
      661 agcgatatta ttacttctga atatatggat ttttgatagg agtagaaatt caagtatagg
      721 ttttagaact aaacgaagtt tgtcttctaa aaaaaattgg gtatactcac aaactatttt
      781 ttatggagga attgttttaa ttagtttact ttcttcaaca ctttattctt tgaatattat
      841 agatgtttcc acctcaaatt caatatctat tattggaatc attattgctg caattattac
      901 acaactgttt ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata
      961 gttttggtga tataagaaaa acatatatgt ataaaaagaa gaaagtagct tctctttgta
     1021 catatatggt ttgtttttat ggatgccgag cttttttata agataaggct accgcccata
     1081 acgacccaag gatttgtgga actaagtaaa tacctgcaag aagcaagatc agctgggtcg
     1141 gttttaaagg atgatagaag taacgccatt cagtaaaatt ggcgcaaaat aaataggaag
     1201 tgacagtaga tataatattg gcaatgaata aagatataat acgcctgttc ctttgaaaat
     1261 aatatccagc aaaaagagat aaaaatatca atgtgatgaa cccgcttacg gaatgataac
     1321 gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa agataaaagc aacggaaggt
     1381 ttttccagca agtttttatc atctttcatc accacctaac aagtgttatt attttgttag
     1441 ttttattgta aattgaatcc tgcaataatc aattactttt tcattatttc tagattaaat
     1501 gaataaactc tcatgccatt atctctaaaa aaaaagtttt tccccctaat ttttttagat
     1561 agaaaattgt atagtatgat gagagaaaaa gtagtttggg ggaacgttaa tgagaagaaa
     1621 agcagtaatc gatcggcttt atttattttt ttggtttttt cctatcgttt tgctaccttt
     1681 tacatgggta aatgctgttg tacaaggtgc ggagatatta ggcctgatcc aaggaagcgg
     1741 atttgtggtt tctaggaatt ttccagcttt gattgttttc tatttagtga taagtgtagg
     1801 tctcttatta ccaaaatctc tgatcaatca ctacaattgg cagctgcttt acttttcttg
     1861 cttattagta tttacatcta tgttttcttt gctgcctcgc ttgattatcg gccctaatgg
     1921 tttgagcgag agtggattga aggatttagc ttattcctcc atgtatttct tgacgatcaa
     1981 accatggttc tatttagtaa ttgcctcatt tattcttgca acattttttt acttctggac
     2041 agaaatcagg ataaaaagtg aacgatctgt atctataaaa aataactatt gatggtggat
     2101 catcagtaaa aaaacatatg agctgcgtaa aatgcatgtt tctcatatgc tttttttatt
     2161 attatgtaaa gaatgcgaaa aacgtttaca tgatttttac ataacttaac tatattttta
     2221 cagcaaccat acgagggatt tacagtcgac tgataaactt tagttgtaaa taagaaagga
     2281 gcaataaaat gaaaaaagta ttgtttactc taggtatgat gggaattctt ttagccggat
     2341 gcggctcagg atcaggaaat acagatggct ctgccacaca aaataattca agtgactcaa
     2401 atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt acagccgtta gtcgatgcag
     2461 cacaagaaag ctttgtacaa gaaaatccaa attatcaaat ctctgtacaa ggaggaggaa
     2521 gcggaactgg tttgagccaa gtcgaagctg gagcagttac tatcggaaac tctgatgtat
     2581 ttgcagaaga aagagacgga gttgatgctt caaaattagt ggatcataaa gtagcagtgg
     2641 tcgggatggc accaatcgtc aataaagata cagatgtcaa agatatcacc aaacaggaat
     2701 tgattgatat ctttacagga aaaatcacta actggaagga agttggcggg aaagatcaaa
     2761 aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg cgcgacattc gaaaaatggg
     2821 gactggatgg tgctacccct gtgcagtccc aagaacaaga ttcatcagga acagttcggc
     2881 agctggtaag tcaaacacca ggagcaatca gctatttagc tttctcatat ctcgatgatt
     2941 ccacacaagc actaagtatc gacggtgtag aaccaaaaga agagaatgta gcagataaca
     3001 gctggggaat ctggtcatat gaacatatgt acacgaacgg aaaaccttcc cctgaagtcc
     3061 aaaaattctt agactacatg atgacagaag aaatccaaga aggacctgtc aaagaattgg
     3121 gctatttacc aatcacaatg atggaagtag aacgtgatca cgaaggaaat atcaaataac
     3181 tagcagataa acatgccacg gtagtcgtta ctattaagga aaaatcaact atgtttcccc
     3241 acatatacat ttttccttta attgtgaaaa atagttcctc atattttgcc gttttttgag
     3301 ctaataccga gggtcagctg attgaacacc gtttattcga aaaaaagagc tacgacatag
     3361 aactgtcgta actttttttg gctcttcgtc aaatcgtgtt ggtgaagaca aatttgaata
     3421 aaacttagtc cgatatgcgg cgcgttctgt gcgccgcata tttttttagt tttcaaagat
     3481 cagaccttgt tgtaaataga tccgtgcccg aaagttataa aagttgcgat aaccaaacgc
     3541 aattcgttta atcagcttga tcttgttatt cagcccctca gtgacgccgt ttgagtggga
     3601 caccttaaag gcatttgaaa tacccgattg gtagcaatgg aagatcgtga atttcttttt
     3661 aaacgactga ggtaacgtag atgaaattgt tttaagggtt tcttgaaagc cttccgtacc
     3721 tcgtttaacg aaataatagc gtaataattg acaaaaatga tacgcagttc gcaattcttc
     3781 gttgtagctt aacagctcat caacgatttc tgtttgacta attggtcgtt taaacagtcg
     3841 atgataatgc cgtttgagtg gctcc
//
LOCUS       DAFBQH010000132         3808 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.139, whole genome shotgun sequence.
ACCESSION   DAFBQH010000132 DAFBQH010000000
VERSION     DAFBQH010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.139"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            92..2191
                     /locus_tag="LK694_002707"
     CDS             92..2191
                     /locus_tag="LK694_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBK5053517.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2387..3250
                     /locus_tag="LK694_002708"
     CDS             2387..3250
                     /locus_tag="LK694_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBK5053518.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3355..>3808
                     /locus_tag="LK694_002709"
     CDS             3355..>3808
                     /locus_tag="LK694_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBK5053519.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         1523 a          636 c          771 g          878 t
ORIGIN      
        1 taaatcctaa aaataagcta ttggcgtgac gtatagaata tgatagatta aatggtagcg
       61 taaacctagt aaaggagccg atcgctaagt gatgaaaaaa tgtccaaatt gcggatcgaa
      121 aaaaatggag cagaatacta tctgtccaga ctgcggtcat caaataactg cagaagatca
      181 gcaaataaaa gaatcaattg aagaaacaaa tacagatgca gctatgcagg ataaatcaac
      241 tgaagatgca gtctttcctg atacagaatt aaacgatcca atcgaatggt cggaattgaa
      301 agatttacct ctagaatctg taatggaatt attcgaaaaa gcagaaccag aagacgaaga
      361 accacctgct cacgatcaag taactaatat agagaaaaat gaacagaaga aagcaactgt
      421 tcatgagact tcggaagaat caggaaagaa tcaagaagaa acgcttgaat caaaacaaaa
      481 aagaagagta gctgaactga aagaaatcgt cgataatgag gaagaaaatt cgatactatc
      541 cgcttatatc aaagcacatc gtgaagatac aaaagaagag cacgcgaaag agctgctcaa
      601 aatgatcagt gaaaaaattg cacaggaaaa caaagtagag ccagaaaagg aacaagaaac
      661 tgaaaaagac tctgatagag aaaacgccac aggtaaagaa acagtaaaaa taaaagacgt
      721 aaaaacggat atcacagaaa aaactgaagt atttactgaa aaacaattag aaaaggaaaa
      781 aacgaataaa ctgccggaag cagagaaaaa agaaatactc gaaaacacag acaaaaagtc
      841 aacagagccc gatgaaataa atagtgaatc cgatgctgaa aatcaatctg taagcaaaga
      901 gggtactgat cttgaagcaa aaatagaaaa agaagagaac cagcctaagc tgtttgaagc
      961 agaagcgaaa tcaataacaa tcgtgccaga tactgaaaaa acacatcaag aatcaaactt
     1021 agaaaaaaca gatccagata aagcgcaaaa gaaaaaatca aaaaaagtac cttatctcgt
     1081 tttagcagca gtacttctac tcggtggagg aagctgggct tattatgacc atcatcaaaa
     1141 agtagaagcc gaagtagcag cagaagcaaa aagacagcaa caaatggaag aacttcatga
     1201 gaaacttgct gccttttata cagatgatca ccatcaattt attcgtgcag atatgattca
     1261 tcaagattta tcaaaactaa aaaatgattt atccagtctt aagaatgaaa aaggatattc
     1321 agagttagaa acaacttacc aagatatcca atctaagatc cgagatatcc aaaaagtgaa
     1381 tgagtggttc atagcacctg ccatcgtcga tgatcatcta gcagatgaca tcaagttgaa
     1441 agcagataaa gagatccaag caatcgaaac ggataacaca gattttggaa aattgattca
     1501 acaagcacaa acagaagcta aaaatcaata tgctgaattg caaacagcta aagaaaaaac
     1561 agctatagtc tttaaagacg gaaaagtagt ggattcagca acacgcgacc aatacaagac
     1621 ggcaaaagaa gcagtagaaa aagtgaaaaa tacagatcta tctgctcagt tgaatgaaca
     1681 attgaaacaa gtagataaga tgctcactga taaagagaaa aaagaagaag aggaaaagaa
     1741 aaaagcagaa gaagcgaaaa aagcagctga agaacaagca aaacaagccg ctcaagcagc
     1801 agctgcagct caagccgctc aacaagctgc ggtcgcacaa gcagcacagc aagaagcggc
     1861 atcctcgacc ccttcctctt caaactcagc gaatcagcca atcaaatctg ccagagcaag
     1921 tgatattgcc gatgcatcta atccagcatg gacgtgggca ccaggtgtcc aaagcaaagt
     1981 gattgctaca tgtatccaac gtggatatat cgttgaagga ggttacagat tagaaaaagc
     2041 cagaatcgaa aatggggaag gctattataa tctatatgcc acttcaacaa aatctgcatt
     2101 gatgaaaggc attggagaaa gtgctttgcc attttacatc gtaaccatca actgtaaaac
     2161 tggatggttc ggaggaaatg ggagcaatta attttaggca aatgaaagag gctgtgagcc
     2221 tgccgtttaa caccgtttat tcgaaaaagg ggagtatgac acaactgttg tcatactcct
     2281 tttttcacct ttccaaacca aggctcagct attcaagtag ataacattat gttatgatag
     2341 caaagaaatg tattaagaaa acctttcact atagagggag aaaaaaatga acgatttatt
     2401 agcaactata tttacaggta ttattattga tgaaaatgaa aaaaactact ttgtccaaaa
     2461 aaacggacaa acctttggct taagcaaatc agaaggtgaa cacaaaatcg gtgaagcagt
     2521 tgaaggattc ggttaccaaa atcaaaaaca tgaaaatcgg atcactacac aaattcctaa
     2581 aagccgtatc ggtcattacg cttttggaat cgttactgct acacgaaaag atttaggtgc
     2641 gtttgtcgat atcggtcttc cagataaaga ctttgtcgta tctcttgatg aattgccgac
     2701 aatgacagag ctatggccta aaaaagggga tcgcttgatg atttccctta gagttgatgc
     2761 aaaagaccgt atttggggca gtttagcaga tgaaaaaata ttcaaatctt taagcaaata
     2821 tggaacagaa gaaatgaaaa acaaagatgt gataggaaca gcataccgct tgaaattaac
     2881 aggtacgtac gttttaacgg aagatttcta tcttgggttt atccatcctt ctgaacgata
     2941 taatgaacct cgtctaggcg agcagataaa aggtcgagtg attggtgtcc gaccagacgg
     3001 tgtactgaat ctttcattga agccaagagc atatgaagct atatcagatg atgcacaaat
     3061 gattttaacc attttagaac gttctgcaga tcagcaaatc aactttacag ataaatcgga
     3121 tccagaagaa atcatgaaag cttttggtat cagcaaagga gcatttaagc gcgcattagg
     3181 taatcttttg aaacaaggat tgattacaca agaaaacggt gtgacaaaac tagtcaaaaa
     3241 atctaattga gaatcccttg catgtttttc ttaactagct tataataatt ttaattaaca
     3301 tagtgttata tcttttttta tttgtaatta ttataaattg ttggggttga gtaaatggtc
     3361 atgaatgcta tttcggcgat gaaaaaaaca aaacagcagc tacatgaagc ggggtttaag
     3421 ctgacaccgc aaagagaagc tactctgctt gtgcttttgg aaaatgagaa agagcattta
     3481 tctgcagaag aaatcttctt tttagtgaaa aaaaagagtc cggaaattgg acttgctact
     3541 gtttatcgaa cccttgaaat cttgacagaa ttgaaaattg tcgataaagt aagttttaat
     3601 gatggattag cccgttatga tttgagaaaa gaaggcgcca aacatttcca tcatcattta
     3661 ctatgcttga actgcggcac gatcgaggaa gtagaagagg atcttttact tgaagtggag
     3721 caagtagtag aagaacgtta tcatttttta gtaaaagatc atcggctaac gttccatgga
     3781 atctgtcagg aatgctatga aaaagaaa
//
LOCUS       DAFBQH010000133         3680 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.140, whole genome shotgun sequence.
ACCESSION   DAFBQH010000133 DAFBQH010000000
VERSION     DAFBQH010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.140"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..276
                     /locus_tag="LK694_002710"
     CDS             <1..276
                     /locus_tag="LK694_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HBK5053520.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="LK694_002711"
     CDS             419..640
                     /locus_tag="LK694_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5053521.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="LK694_002712"
     CDS             656..1498
                     /locus_tag="LK694_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053522.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="LK694_002713"
     CDS             1561..3363
                     /locus_tag="LK694_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HBK5053523.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYCWKYKD"
     gene            complement(3422..>3680)
                     /locus_tag="LK694_002714"
     CDS             complement(3422..>3680)
                     /locus_tag="LK694_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBK5053524.1"
                     /translation="GITISIPPRKNMDKSEKLDHSLLGKQRKTVGTVFSLLEKLGCQN
                     FNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1311 a          523 c          749 g         1097 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actgctggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaataga tacccgtggt gctagttaat
     3421 tttaatatcc taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata
     3481 gaacactgta agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg
     3541 agttgaaatt ttgacaacct aatttttcta acaaagaaaa aacagtccca actgtttttc
     3601 tttgttttcc aagtaagctg tggtctagtt tttctgattt atccatgttt ttacgtggcg
     3661 gaatggatat agtaatgccc
//
LOCUS       DAFBQH010000134         3586 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.141, whole genome shotgun sequence.
ACCESSION   DAFBQH010000134 DAFBQH010000000
VERSION     DAFBQH010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.141"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            116..976
                     /locus_tag="LK694_002715"
                     /pseudo
     CDS             116..976
                     /locus_tag="LK694_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
     gene            988..1815
                     /gene="panB"
                     /locus_tag="LK694_002716"
     CDS             988..1815
                     /gene="panB"
                     /locus_tag="LK694_002716"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBK5053525.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            1830..2678
                     /locus_tag="LK694_002717"
     CDS             1830..2678
                     /locus_tag="LK694_002717"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBK5053526.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            2688..3083
                     /locus_tag="LK694_002718"
     CDS             2688..3083
                     /locus_tag="LK694_002718"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBK5053527.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            3285..3586
                     /locus_tag="LK694_002719"
                     /pseudo
     CDS             3285..3586
                     /locus_tag="LK694_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1399 a          397 c          715 g         1075 t
ORIGIN      
        1 gatattatga taaattaact atggatgata tttgaaaaaa acatactgta tatttgattt
       61 aatttttatt tttaagctca tatcgtaaga atgggacttg tagtctcact tctttatgga
      121 ggtgataatt atgaacgttg gttttattgg agccggcaag gtaggttgtt cattcgggaa
      181 gtatttccag gaacataaaa tacaagtaac aggattttat agtaaaagcg aagactcgtc
      241 attagcagct tcgaatttta catctagtaa acaatattta aacttaagag aattagtaga
      301 tgaaaatgat actattttca ttacgactcc tgatggtcaa attcaggagg tatggcaaga
      361 aattaaaaat tattaaatca aaaacaaatt aatctgccat tgcagtggtg caatatcttc
      421 agatatcttt tcaaatatac aggactatgg tgcttatggc tattctgtcc atcctatgtt
      481 ttcaatatca gataaatata attcatacaa aaaattaaaa aaatctttta taacaattga
      541 aggcgatgaa aaatatgcaa agtttttatg tgatttcttc aaaaagttag gtaatagtac
      601 tgtaattgta tctaaggaga ataaatctct ttatcatgcc gcatcagtag tatctagtaa
      661 ccttgtgcta ggacttatta atacaagcgt tacatatcta attcaatgtg gatttactga
      721 aaaaatggca attgaagctt tatatccatt aattgaattt aatattagaa atataaaaga
      781 aaaaggaatt atagaaagtt tgacagggcc agttgaacgt tgtgatattt caacaattaa
      841 aggtcactgt gaagttttat cagatgaaga cagagcttta tatatgctat tatcaaaaaa
      901 tgtattagag atagctatgt taaaaaatat taatagagat tatagtgaat tagaaaagta
      961 tttaggagag agttaggaga gaaatagatg aaaaatacag cagttacatt taaagaatct
     1021 aaattaagaa atgaaaaatt aacaatgctt acagcttatg actattcaac agcaaaaata
     1081 attgatgaag ctgggataaa tggaatatta gtaggagatt ctcttggtat ggtatgtcta
     1141 ggacatgaag atacactttc agtaacaatg gaagacatga ttcatcatac tagagcagtt
     1201 acaagaggag ctaagaacac tttagttgtt gcagatatgc catttatgtc ttatcaaact
     1261 tcagtatatg attcagtagt taatgcagga agattaataa aagaaggacg agctcaggtt
     1321 gttaaattag aaggagggat agaagtatgc gataaaatag aggctatagt aaaggcttct
     1381 atacctgtta tggcgcatat aggtttaaca cctcaatctg ttaatgcatt tggaggattt
     1441 aaggtacaag gaaaggacaa ggaagctgct aaagaattaa taagagctgc taaagctgta
     1501 gaaaaagcag gagcattcgc tgtagtgtta gaatgtgttc caactaagct tgcagagtta
     1561 atttctaaag agatatcaat acctacaatt ggtattggag caggtgcagg gtgtgatgga
     1621 caaattcttg tatatcaaga tatgttgggg atgtatagtg attttacccc taaatttgtg
     1681 aagaaatatg ctaatttatc agaagagatg aataaagcat ttacaaaata tatagaggaa
     1741 gtaaaagatg gagtttttcc aggtccagaa catgggttta aaataagtga tgatgtttta
     1801 gaaaaattat attaaaaggg tcaagaaata tggttatatt taaaacagtg aatgatgtta
     1861 gaagtcaagt aaaagagtgg aaaaaacaag gcttaaaggt aggattagtt cctactatgg
     1921 gatatttaca tgaaggtcat gaaagtttaa ttagaaaagc atcaaaagaa aatgataaag
     1981 ttgttgtaag tatctttgta aatccaactc agtttgggaa aaatgaagat ttaggtagtt
     2041 atccaagaga cttagaaaga gatatagaag tatgcacaag aggtagagca acagcaatat
     2101 ttaatcctga agttgaagag atgtattgtg acaatgcaag tacttttgtt aatataactg
     2161 gattaacaga aggtttatgt ggagctagta ggccaataca ttttagaggg gtttgcactg
     2221 ttgtaagtaa attatttaac ataataccag cagatagagc ttattttgga gaaaaggatg
     2281 cacaacagtt agctgtaatt aaaagaatgg ttagggacct taatattgat atagacgttg
     2341 tagggtgtcc aattataagg gaagaggatg gactagctaa aagttctaga aacacatact
     2401 taagtttaga ggaaagatct tcagctacta ttttaaataa atctttaact ttagctaagg
     2461 aagctttaaa taatggagag agagacagct taaaaataat tgagataata agtaaaaata
     2521 taaatactta taatttggca aagattgatt atgtagaagt tgttgattct ttatcattac
     2581 aaagagtaaa ttatattgaa aaatcagttt tagttgcaat agcagtattt ataggaaaaa
     2641 caagattaat agataatttt acatttgaat tacaataggg ggctataatg aaagttacaa
     2701 tgttaaaagg aaaaatacat agagctacag ttgaacaagc tgagttagat tatgtaggaa
     2761 gtattactgt tgatatgaat ttattagaag cagcaggaat atatgaatat gaaaaagtac
     2821 aaatagttga tgttaataat ggaaatagat ttgaaactta tacaatagct ggagagagag
     2881 gaacagggat gatttgcttg aatggagctg cagctagatg tgtatcaact ggagataaaa
     2941 taattttaat ggcatattgt gaattaaatg aaaatgaggt taaggatcat agacctaagg
     3001 ttgtttttgt cgatgataat aataaagttg aaagagtaac atcatatgaa aaacatggaa
     3061 ggttatcaga tataattctt taggttttgt aatgttattt atatatagct agactattat
     3121 tgaaatattt attgattact aattggatag atgtcaataa agtagacaca aaaagagaag
     3181 atacagactg tagacaaagt ccattttatg tgaatcggct acactagact agacagaaaa
     3241 aataaggtgt gtagaataat aaaaaacaca ctggaggatt tttcatgtca agacgtcaac
     3301 gaagaaccta ttcaaaagaa ttcaaacaac aaatcgtcaa tctctatctc gctggtaagc
     3361 ctcgcgcaga aattattcga gagtatgagc ttacgccttc ttctttcgat aaatggatga
     3421 agcaagcaca atcaacgggc tcattcaaaa aaagagacaa cttaacacca gaacaagcag
     3481 aattgatcgc actaagaaag aaaaataagc aactcgaaat ggagaatgat attttaaagc
     3541 aagcggcgct gatattcgga cgaaaagaca agtaattgat gccaac
//
LOCUS       DAFBQH010000135         3393 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.142, whole genome shotgun sequence.
ACCESSION   DAFBQH010000135 DAFBQH010000000
VERSION     DAFBQH010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.142"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(69..2561)
                     /locus_tag="LK694_002720"
     CDS             complement(69..2561)
                     /locus_tag="LK694_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBK5053528.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2568..3035)
                     /locus_tag="LK694_002721"
     CDS             complement(2568..3035)
                     /locus_tag="LK694_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBK5053529.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(3170..3243)
                     /locus_tag="LK694_002722"
     tRNA            complement(3170..3243)
                     /locus_tag="LK694_002722"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3207..3209),aa:Arg,seq:acg)
     gene            complement(3301..3374)
                     /locus_tag="LK694_002723"
     tRNA            complement(3301..3374)
                     /locus_tag="LK694_002723"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3338..3340),aa:Pro,seq:tgg)
BASE COUNT          928 a          750 c          557 g         1158 t
ORIGIN      
        1 aaaaatcttg tcacaacctc ttaatcaagt cttactatta taaaaattca gctacacagg
       61 ccaagtaact atagggaacc tactgtttcc tttttaggtc cgcgaacatt caatgtgatc
      121 ttaccttttg aagcacctac tgttacgtgg tcacctaaac gaatttctcc gccaagcaaa
      181 gcctcgctca atcgatcttc aatttctttt tgaagtgcgc gacggattgg acgtgcacca
      241 tattcaggat caaatccagc tttcccgatc acatcgattg cagatggtgt gattttcact
      301 tgaatatctt gttctttcaa tcgtttgata atcgctttgc tcatgatttg gacgattgta
      361 tggatttcat ctttactcaa tgaatggaag acgacagtct catcgattcg attcaagaat
      421 tcaggtcgga aagctttctt taactcttct aagatacgtt tttgcatggc tttgtgatct
      481 ttagtaacat cttggacatt gaatccgaca tttttttctt ctcgaatttg tgtcgctccg
      541 atatttgaag tcataatcat gattgtattt ctaaagtcca cttttctacc tttagaatct
      601 gttaaatgac catcatccaa tacttgcaga agtaaattga aaacgtctgg atgtgctttc
      661 tccacttcat ctaagagaat cactgaatat ggtttttgac gaattttttc tgtcaactgt
      721 ccaccttcat cataccctac gtatcctgga ggagacccga ttaatcggct tgtactgtat
      781 ttttccataa attctgacat atcaacacgg atcagggcat cttcacttcc aaacatgact
      841 tctgaaagtg ctttggccaa ttctgttttt ccgacaccag ttggtccaag gaacatgaat
      901 gaaccaattg gacgatcagg atctttcaat ccgcttcgtg cacggcggat cgcacgtgaa
      961 actgctttga cagcctcatc ttgaccaacg acacgttcat gcagaagtcc ttccagttct
     1021 aacaagcgtt cgctttcttt tttctccaac tgctgtagag ggacaccagt ccactccgat
     1081 acaactgtag caacatcttc ttctgttaca cgatcagaat aaccttgctc ctgttttgct
     1141 tcaattgcag aaactttttg cagcttttta cgtagtgctt tttcttgtct gcgtaattga
     1201 gcagcatttt caaagtcttg tttttggatc gctgcttcct tttcttgtac tagttcatcg
     1261 atttctagtt tgatcacagt tgattttgtt aagtgatcgg tttgatccaa acggactttc
     1321 gctgctgatt catcgatcaa atcaattgct ttatcgggta gttgacgaga agtgatataa
     1381 cgaacagaaa gattgactgc tgctcgtaca gcttcatctg tgatctccac tccatggtgc
     1441 tcttcatatc ttgtacgtaa tccttgaaga atcacttctg cttcttcagg agttggctca
     1501 tctacttgaa tgcgagcaaa acgtcgctct aatgcagaat ctttttcgat atatttttga
     1561 tattcatcta gggtcgttgc tccgatcgtt tgtaattctc ctcgagctaa cgccggtttc
     1621 aagatattcg aagcatcaat tgctccttct gctccacctg caccaattaa ggtatgcaat
     1681 tcatcaatga ataagattac ttgaccatca ttatagattt cgtcgatgac ctttttcatt
     1741 cgatcttcaa attcaccgcg atatttcgta ccagcaacca aagatcccat atcaagcatc
     1801 atcaggcgct tttcttgcat gtcttctggc acttcaccta aaatgatttt ttgagctaac
     1861 ccttcagcaa tggctgtttt gcctacacca ggttcaccca ccagtacagg gttattcttt
     1921 gtccgtcggc taagaatctg gataagccgt ttcacttctc tcgaacgacc aacaactggg
     1981 tccagcttgt tttcttttgc taatttcgtc aaatcacgtg ctaatgaatc tagcgtaggt
     2041 gttccttgcg gtgtttgccg tttatcttgt acatttacat ttctacgacg attcattcct
     2101 tttccagcag aatcaccagc tagccccatt ttttgtttca gaacttgacg ggcttttgaa
     2161 aggctgatac ctaagttgat catgattcga gtagccatga tactttcatc ttttaataag
     2221 ctcatcaaaa tatgttctgt tccaactttt tgagaaccag ttttcttcac ttcgctgcta
     2281 gctaaagcaa aaacttgttt cattcttggt gaatatggaa gaaagactcc tttagggtaa
     2341 gaacggatac caccataacc aatcagatgt tcaatttctt cataaatttc cgtttcattc
     2401 aaatccattt cacgtaatac ttttccagca attccatttt gctcgaccac taaggctagt
     2461 aaaacatgtt ctgaactaac tgattgatgt ttcaaccgtt ttgcttcttc ttgtgcaatt
     2521 tctaatactg cttgggctct ttcagtaaaa agttcttcca tctaatctca cttcccatct
     2581 tcataactta aacgttccag aaatgctcgg agaatccgag cacgtgtatg atcttcatat
     2641 tcattgaaat tcaatgtgtt ttttgcaatt gcactcaaca tcaagtttcc ttcttttttt
     2701 gtaattattt ggtcttcata aagtttttga ataatagcaa aagcattttt ctcactgatt
     2761 gtatcatcaa ataactgatt tatctgctca agtaattgtt ttttatctga aatccttact
     2821 tttgcaattc gtatataacc accaccacca cgtttgcttt ctactagata accacgttgg
     2881 atggtaaaac gggtattaat cacatagttg atttgtgaag gaacacagtt aaagaggtct
     2941 gccatttcaa tccttcgaat ctcgatcatt tcactatttt ccagaatttt ctttatatat
     3001 gattcaatta aatccgaggt attctgatgt gccatcttat tcattccctt tgactaatat
     3061 tgaccttaat tataacgaat ttgataatag aaagaaagaa gaacgctttt tgttaaataa
     3121 atcctttaga atcaacaaaa aaaatacgga atatactatt ccgtatcgac gcgcccaaga
     3181 ggagtcgaac ccctaacctt ttgatccgta gtcaaacact ctatccagtt gagctatggg
     3241 cgcatattca atttaaaaaa attataaatc atttgaaaac aaaaagcaag ataaaaaaaa
     3301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
     3361 tgagctactt cccgtttaaa aaataaaaaa gac
//
LOCUS       DAFBQH010000136         3374 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.143, whole genome shotgun sequence.
ACCESSION   DAFBQH010000136 DAFBQH010000000
VERSION     DAFBQH010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.143"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="LK694_002724"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="LK694_002724"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBK5053530.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="LK694_002725"
     CDS             complement(2261..2512)
                     /locus_tag="LK694_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBK5053531.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="LK694_002726"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="LK694_002726"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBK5053532.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          920 a          740 c          626 g         1088 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttt
//
LOCUS       DAFBQH010000137         3292 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.144, whole genome shotgun sequence.
ACCESSION   DAFBQH010000137 DAFBQH010000000
VERSION     DAFBQH010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.144"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            111..1100
                     /locus_tag="LK694_002727"
     CDS             111..1100
                     /locus_tag="LK694_002727"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBK5053533.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(1417..1941)
                     /locus_tag="LK694_002728"
     CDS             complement(1417..1941)
                     /locus_tag="LK694_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HBK5053534.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            2228..>3292
                     /gene="scm"
                     /locus_tag="LK694_002729"
     CDS             2228..>3292
                     /gene="scm"
                     /locus_tag="LK694_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBK5053535.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         1047 a          667 c          611 g          967 t
ORIGIN      
        1 gaacgcccac cctcttcttt tttctctttc cttctttcgt ttttgcttta tgtatcaacc
       61 gttgtttctt cgaaaactat tttgttctct ccttcttcct gatagaatgg atgggaaagg
      121 agcttgatgc catgacaacg aaagacaagg tacttcaatt attgaaaaag acaccagatt
      181 ttctttccgg agaaaagttg gcacagcaac tggaagtttc ccggacaagt atttggaaag
      241 ccatcaaaga attggaaaag gcaggctatc gttttgagca cgggcctaca gggtatcgtt
      301 acctgccctc tgacgtacta gatgcaaaag aaatccagca agagctacgg tcgtttattc
      361 ctgatttgaa agtacaaacg atgttgactt ctgaatctac gatgaaagat gccaaactgg
      421 cagcagctca aggagattct gtttcgaagt tatttgttgc tgatacacaa gaacaagctc
      481 gtggacgttt tggacggcct tttttcgctg aacctggacg aggaatctac atgagtctct
      541 tgctccatcc agacaaacgg tttgatgaat tgcctcaata tacgatcatc gcagccagtg
      601 cctgtgtgaa agccattgaa tctgtaaccg gtcgttctgc ttcgatcaaa tgggtcaatg
      661 atatctacat ggatggaaaa aaagtctgtg ggattttatc tgaagcagtc agtgatatgg
      721 aaagcggcag gatcagtcat atcatcattg ggatcgggct taatttttct gttcctcaaa
      781 atcattttcc agctgatctc caagaaaaag ccatctctgt ctttcccgac ggacaagcta
      841 gtgtcacaag gaatcagcta atcagcaaaa tctggcaaga attgttcact tatctttctc
      901 acccagaaaa agcgttggac ttctataaag aaaaatcatt cgtattggga aaaaccgtgt
      961 cgttcaatca aaaaggaacg acttatgtcg gtcttgcaaa agaaatcggt gatcatggcg
     1021 aactgatcgt ccaaacaaat gaacagaaga tgagtttgtc ttctggcgaa atcagtctca
     1081 catctatccg tacgcaataa ttatcagatt tcacaaaagg acacctgatc tcatacagtg
     1141 tccttttttc tgaattctct tcaaaagact ctccttattc tatcagctga cagtttctct
     1201 atcctctaga aaaaggtatt ctaaaaatat ctataaagca tggtacactt tctttaaatg
     1261 aatcaatgca aaaaacaaga gagtcatcag tttacacgat caaaagagat agcaggccgc
     1321 ttcctttttc aatcttatag atagaccatg ggtaacgata aaaacaaaaa agaacttgcg
     1381 acttattttg ccgtaagttc ttttttagct agtgccttaa tctcttaatc ccaacaaacg
     1441 tctcgcatag atcacctgac cagagtgcat agcagcatca tcaataatag aaacgagacg
     1501 cattcctcgt gtgactggtg gtgtccagtt ctcgtcaatt acttcatcta gctcggaagg
     1561 ttccagcctt cttaagtaag aaatagcaaa atcagcagct cctcgcaagt atcctagcag
     1621 atcttctatt tgattagctt ggatctgctt tgcttgttcc agactatgct tccaatcttg
     1681 ttcgttatct gctatcgtca tcggaaaccg gctttcccag tcttgactat gccaaattgg
     1741 agaagttcct gctaaatcag caatctggaa atctaattct cgagcggtat gccaagccag
     1801 ccaagtcatt gatttgatct gcggggctct atcttccact ggaaaacgat tggcctcctc
     1861 aatagagacg ccttcaaaca aacgttcaaa ccgatcgtat gcccgatcca cactctcgca
     1921 tagcatatct aaataatcca tttattttcc tcctttttat ttatcctact tactttttcc
     1981 taattctagg atacctactt atatgttact acacaccctt attttttaca ctttaacagt
     2041 caccatcatc aatagtttgg agattgaaag aaaaagatag tgtcgaacta tcaattgaac
     2101 aagaagcaac aatatgaacg tttcattaat attctttaaa taaacaatga tttttcaaac
     2161 gaacagttta agactaggag aaaaagagta taataggaaa atgtttgaaa ggaaaggaag
     2221 gggaaaaatg aaaaaaaggg caagtttact agtcctagtt gctttgttgt tcaatatctt
     2281 ttcgtatggt gtggccgttt cagcggaaac atacattcct aaagaccctg ggaatattta
     2341 taatgatcta actggtaact atggcttgtt aggaattgct agtcaattcc acgtatttgc
     2401 taaaaataag acaactgtga atgcacatac agacggaaat atagcaacac atgaacttga
     2461 tgcaaaaaat aattttggta cagagcttca tgaaggttta ttgaaccaag aaataaatta
     2521 tatacaaaaa actgattctt tcgctaattc ttcaggcata cctacaggta gcacaagaat
     2581 gaataaattt gtcgttggca gtaacgtgga tgtagcactt caagatggtc atcctacagt
     2641 caatggatcc aggcttgatc acttaactcc agatgatttt tataaagatc gtgctgggca
     2701 tgaatatatt gatttcgagc aggaatttgc taaattgaat gtagcagcaa atgatcttgc
     2761 aacgatcact ccagctaaaa catatactgc tgcagatttt ccagacatga acaatcggac
     2821 aatcgattta actgatttat cagataccgg gtttttattg gtaaatatcg atgcagaagt
     2881 gttaacgatg aatacgccac ttcaaatcat taatccaaat gatcaagtag ttgtatttaa
     2941 tgtcatcaac agttcttctg cattgaacgt acagtcacca atcaaataca atgatcgttc
     3001 aaatcatgaa acagaagatt tttcggatgc gaatatctct tggaattttg gaaatgagat
     3061 gactgatttg acaatcagtg ctccttttca aggaacaatc cttgcgccta atgctactat
     3121 ccgcattact caaaaccaag acggaacgat tataggaaca aatgtgattt tagatgcagc
     3181 taccaatcgt tgggatccta acgaaatatt tatcagcgac aatggtacag atacgacaga
     3241 cacaagcgac agtacagata catctaccac aagtgatagc tcagattcat ca
//
LOCUS       DAFBQH010000138         3232 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.145, whole genome shotgun sequence.
ACCESSION   DAFBQH010000138 DAFBQH010000000
VERSION     DAFBQH010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.145"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            184..1138
                     /locus_tag="LK694_002730"
                     /pseudo
     CDS             184..1138
                     /locus_tag="LK694_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
     gene            1155..2179
                     /locus_tag="LK694_002731"
                     /pseudo
     CDS             1155..2179
                     /locus_tag="LK694_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            2185..2664
                     /locus_tag="LK694_002732"
     CDS             2185..2664
                     /locus_tag="LK694_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBK5053536.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            2680..>3232
                     /locus_tag="LK694_002733"
     CDS             2680..>3232
                     /locus_tag="LK694_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBK5053537.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVG"
BASE COUNT          980 a          539 c          751 g          962 t
ORIGIN      
        1 acttggaaaa cgttgtttct ccaagttctt tttttaattt ccaagtaact gaaagttact
       61 tttgttattt aacatttagg tcaattttag acagtttttg ttcaaaaagc gatatttata
      121 gtggacggta ttactgatat attaaggata taaaatgaaa gcgtttaaaa aggtggcgta
      181 aagatgatta ttaaagaaga gatttttgta cctaaagaat tagtgaaaga aaaaattgac
      241 ctgctcgttg aaaacctgac aacgatcaaa gacgataatg gtgaattttt gttgggcttt
      301 gatggactga aagtagatga caagagctgg acagtgtgga attggccaca aggtgtcggt
      361 ctatatggta tttataaaaa ttaccagatg acaaaaaatc ctcgcgctta tcaagtggtc
      421 aatgaatggt ttgaggatcg tatggaagaa ggcgctccgc ccaaaaacgt caatacaatg
      481 gcaccgctat tgactatggc ttacttgtat gaagacacga aagattcacg ctatttgcct
      541 tatttagaac aatgggcaga atgggtcatg aatgacatgc ctcggacaaa tgaagacggg
      601 ttacagcatg ccacatatgg accggaaaat aagaatcagt tgtgggacga tacattgatg
      661 atgacagtgc ttcctttggc aaaaatcggg aaactgctgg ttgacgattg ctattccaga
      721 gatcattgaa atcttagaat tatcgaaaga agatgcattg cgcaaattat tgatcgaaac
      781 acttgaagca caagtgcgtg cattaaaaac ttatcagtct gaaagcggtt tatggcatac
      841 gttgttggat gatccttctt cttacgtaga atcttctgca actgcaggat ttgcttacgg
      901 tattttgaaa gcggtccata aacgttattt accacaagaa tataaagaag tcgcttatcg
      961 tgctatccaa ggattgcttg aacaaatcga tgaaaaagga gaagttcaaa acgtttcaat
     1021 cgggacagga atgggggaca gtttagattt ttatcggaat atcggaatca cagcaatgcc
     1081 ttatggacag tcgttaacgg tgctttgttt gacagaattg ctcgtctctt attgttaaaa
     1141 gaaaggaaac ccatatgaaa aaaacatcac tgctgtttgc agcaagttcg cttagcctag
     1201 gtctgctagg tctaagcggc tgcggaaata attctagtgt ggcaactgtc gcagatgcag
     1261 acgtaaatga gatagttacc ttgcgctttg cttatgcaag caacagccag taattgatgc
     1321 aatgaatgaa tttgggcgtt tagtaaaaga aaaaacagat ggttccgtac agatccagta
     1381 tttcccagat ggacaactgg gtggagaacg tgaactgatc gaattgacac agtcaggagc
     1441 agtagatttc acaaaagtaa gtgcctcagc gttggaaagt ttctctaaag attattcgat
     1501 tttttccgtt ccttatctat tcaatgatga agaacatttc tttaaggtga tggataatga
     1561 agaaataatg gagccaatct accaatcgac caaaaattta ggatttaccg gattgaccta
     1621 ttatgattct ggtcagctca gcttttatat ggtagacggt ccgatccata cgccggaaga
     1681 tttgaaggga aagaagatcc gtgtgatgca aagcgaaacc gctatcaaaa tggtagagct
     1741 tttaggcggt tcgcccgtac ctatgggaag tgatgaagtg tacacgtctc ttcaatccaa
     1801 tttgatcaac ggatcagaaa acaatgaatt tgtcttgtat acagctggac atggcggggt
     1861 agctaaatac tattcctatg atgagcatac acgtgtgcca gatatcatca tcatgaatga
     1921 tgccatcaaa gaacagctaa ctgatgaaca ggaaaaagcc attgaagaag cagcgaaaga
     1981 atcaacggtt tttgaaattg aagcttttgc aaaagcaatt gaagaagaaa aagctttagc
     2041 acaagaagaa catggagtcc aattcaatga tgtagataat acaccgtttc gagaggcagt
     2101 tgccccactt catgatatat tcaaaaacga tcctacctac aaagaccttt atcaaaagat
     2161 ccaagagcaa ggagcgtagc tgtaatgaaa atgattttaa acaaggtatt agagatcctg
     2221 ggagctgttt tgcttgttgg gatggtcgct gttgttttat ggcaagtatt ttccagaacg
     2281 gttttaggaa atccaaatac ggtcacggaa gagcttgttc gattcggtct tgtctggttt
     2341 gccatgttgt cttccgcgta tgttgtggga caaaaagcac gtttagccgt aacggtttta
     2401 agtgaaaaat taactggcaa aaaaagtaaa attttagaag ttgttgttca aagtttattt
     2461 ctggtatttg ctgcgatgat catggtgtat ggcggatgga atgctgtcac attgacaatg
     2521 gggcagatct cgccttcttt aagtattcct atgggatatg tttatctgtc agtacctgta
     2581 tcgggggtac tcatcattat ctatagcctg atcaatctaa tcgatacatt aagcaacaag
     2641 ccagcaattc aagaaaaaat ataaaaatgg aggaataaaa tggcaataga agccggatta
     2701 gtgttatttt ttgtatttgt tgtgttgctt gtattaggga tgccgattgc tataagtatt
     2761 gcattctcat cgatgatgac tttattgtta gttataccgt ttgatgtttc tgcttttagt
     2821 tcagctcaaa aaatggtggg aagtatcaat agtttttcgc tgatcgctat tccgtttttc
     2881 gttctctcgg gcattatcat gaataatgga gggatagcta agaaactagt ggattttgcg
     2941 atgttgttcg tcgggcgcgt gccgggggct ttggctcata cgaatgtgtt agggaatgct
     3001 ttatttggtt ccatctctag ctcggccatt gccgcttcta ccgcgattgg tggagtgttg
     3061 atccctcagc aagtggaaga aggatatgac cggaagtttg cgacagcggt caatatcgcg
     3121 tcagctccga caggtatggt cattcctcca agtactgctt ttatcatgta ttcacttgtt
     3181 gctggtggtg cttcgatctc tgctttgttt atgggaggtt atctcgtagg cg
//
LOCUS       DAFBQH010000139         3079 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.146, whole genome shotgun sequence.
ACCESSION   DAFBQH010000139 DAFBQH010000000
VERSION     DAFBQH010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.146"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(1014..1304)
                     /locus_tag="LK694_002734"
     CDS             complement(1014..1304)
                     /locus_tag="LK694_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053538.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2203)
                     /locus_tag="LK694_002735"
                     /pseudo
     CDS             complement(1512..2203)
                     /locus_tag="LK694_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
     gene            2598..>3079
                     /locus_tag="LK694_002736"
     CDS             2598..>3079
                     /locus_tag="LK694_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK5053539.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     G"
BASE COUNT          931 a          598 c          454 g         1096 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgatttct acatttgttt
     1681 tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt tgtaattctg
     1741 aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga tgattgattt
     1801 catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt ttgctttgta
     1861 gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct gccgattgat
     1921 ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct gcctttgaat
     1981 cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc gcttgctctt
     2041 tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca atatctgtct
     2101 ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca attagacata
     2161 gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata gctccttaat
     2221 ccaatacttt tacatgctaa aagtaagtga tctcaaacac aatgtcaagc gtttttaaat
     2281 tacatatgta attttaatat aaatactatc aaatactcat ttaatcctcc ttttttatct
     2341 atttaaaaaa tcttttttat agagaaaaat gcaggttaat actttcttaa ataaatattt
     2401 atggtgaaca aaaacaaaaa agcaagtttt attaaacgta acccttttca tttcatagat
     2461 tactgtattt attacaaaaa acgcggattt gtcataaatt gtcatgcttc ttttacctat
     2521 gaaatatttt cacgtgttaa agtattcctc gttgaacaca acaaataaaa ctttttaacg
     2581 aataaaggag atttttcatg acatcactta aaactttact ttttggaaca actttggctg
     2641 ccggcgctgc attcttcatg ggaacgactg ctcatgcgga cgaagcttat accgttcaat
     2701 caggtgatac tctatcaacg atttctcaaa aatacgttgg tgataactcc ttgatcaatg
     2761 ccattgcaga atcaaattca atttctgata ttaacctgat ttactcagga caacaattaa
     2821 caatcccaac agaaggctct gcacaagcag ctgctgaacc tcaagcagcc gttcaagaag
     2881 caccagtcca agctgaacaa cctgttgtac aagaaacggt tcaaacggaa aaacaagcag
     2941 cgcctgttgc tgaaacacaa cctgctcctg ctgtaacaga aactgctgct actccagcaa
     3001 gcacaagctc tgcaaaagaa tggatcgcac aaaaagaatc aagcggttct tacacagcaa
     3061 cgaacggacg ttacatcgg
//
LOCUS       DAFBQH010000140         3067 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.147, whole genome shotgun sequence.
ACCESSION   DAFBQH010000140 DAFBQH010000000
VERSION     DAFBQH010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.147"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(25..162)
                     /locus_tag="LK694_002737"
     CDS             complement(25..162)
                     /locus_tag="LK694_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBK5053540.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="LK694_002738"
     CDS             complement(159..1343)
                     /locus_tag="LK694_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBK5053541.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1602..1892)
                     /locus_tag="LK694_002739"
     CDS             complement(1602..1892)
                     /locus_tag="LK694_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053542.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(1882..3024)
                     /gene="dcm"
                     /locus_tag="LK694_002740"
                     /pseudo
     CDS             complement(1882..3024)
                     /gene="dcm"
                     /locus_tag="LK694_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
BASE COUNT          838 a          734 c          537 g          958 t
ORIGIN      
        1 aaattttgtc cgaaattcat cttgtcactt tcctttcttt tccattgctc gatccgctgc
       61 tgcacttgca ttcatcaagc acatcacagt aacaccaagc atactgccac caaataccaa
      121 aaaaatatct tttacaattc ccatgacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatcttttta tgtcggtctg ataagcgtgc attctgtacc
      241 atatcctgga tataggttgt ccgattcaat cgatccaact ccttagccat tttccacgta
      301 ggggcgactt gtcggcctag ccaattcaag gttcgttcaa tggtatacgg ttttggctcg
      361 gaagttaatt gaatctcttt ccgattccgt ccaataaaac gcccccattg ttcattcaag
      421 ggccagttgg ttcgcctttt accttctact ttatccgcaa atcgcaagta acgattgata
      481 atggaaaacg tggtgctctc aatgctacga ccttttaaca aatcttgaat cgcatgaaaa
      541 gcacggtcat ttttcagtcg aatctcaaaa cgatttttcg tctcagtttg gtctaatgga
      601 atccctaact tgatatattg ttcatagtct ttttcataca aacagaaata aacctcactc
      661 ttgagactgc cgatatataa ggtattgccc atgccgtcct tttcatcagc ctttaaaagt
      721 tccccggaac gataactttt aaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta gctcaggtat atccaatagc cccactcgat cattgattgc caagtccaag
      841 cgtttcatca cgccaccggc ctttagacaa tcctcgaaaa agtcgtacca gctacgcttt
      901 tgagcgagta aaaaagtttc aaattgtcgg caacctcgcc cctttaattc taaaagcaca
      961 cctttatctt cttcatgaga caacatcacc acaatatcac ccatgacata ctgttcttga
     1021 taggagtaaa aggcataatc ttcatggagc atatactcaa atttcaaatg gagaatttct
     1081 tgaataattt ttcgtgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gtcaaaggca aatcgggatt aaagctttcc agttggtcaa aaattttcct catggttttt
     1201 atagatgcgg ttacgccacc attttctatc tgatttaagt acggccgact aagtccacaa
     1261 gccgtagcca atttattttg tgaaacgccg tattcgttgc gtcggtcttt taatagccga
     1321 taatctaaat tccgttgtgc caagttctca cctccaaatt caaggtgtta actttttcaa
     1381 atgagttaac acctatacaa attacttact atctttgata caacaagcat tcttaatggt
     1441 tgcttactta ttttttgtgt ttttgtaccc ccctgttaga tacgggggtg agacatcatg
     1501 gtcggctaac gccgtccatg aacaggcaca gcaccacgga ggatttctgc ttcgcgaatc
     1561 ctccgcggtc agcgcctaac agtcagttgt gttggtttag gttactcatc tgtaatacca
     1621 tttacttcca actctgcttt catttctaac aagtattgac gaatatctgg ttcttttgct
     1681 tggtccgcaa tccgctcaaa ttctgcaatt acttgattta tttcaattcg agcatccgtc
     1741 tcctcattca ttaaatatgg atcgccagcg actttgacac cattcttgcg aatatcaaat
     1801 tttaaccgtc gcaattgttc ttctaattct gggtctgact gaatctttcc aatcacccaa
     1861 taaatttctc caatcaactc atcataaacc atgtacatcc aactctttta atcttctggc
     1921 aatatcaaaa atgaccggaa ctgttacaga gtttccggct tgcttataca actgactatc
     1981 tgaattgact tgagaatctc gatcaaaagc ccaatcggga aagccctgaa gtcgccaaca
     2041 ctctctaggt gttagcttcc gaatttgata ctgatccgtg acgatcgctt gttggtctcc
     2101 tgttagtaag gtatgaacac tctgttttcc cagacgacct cggcgacttg tggaattagg
     2161 aaaagccaga ttcacactgt cttctggctt cacctcttga tattctgttt cattttttcg
     2221 aatcatgatt tttgtaccac cttgtttcgt tgacaaggtg ggggcggttc cagtaacttc
     2281 ataaactcga ttaccttgtt caaactttcc cggtaatagt cctgctacag agattccatc
     2341 ccttgctggc agtgcgattt tgattccttc atcacctgtt aaggttggcg ctacgccatc
     2401 tgaaccatag acttgtccac ccattcctcg attggaagga ttgatattta ccaaatttct
     2461 gatgttttga tcagatgttg aagacggtcc aatgatagga aatacttgtt cggtacatgc
     2521 gtctcgagaa tgtccgataa ggaatatccg ctcccggctt tggggaatgt aggcctttga
     2581 gttaagcacc taccactcca cgtcataccc caatccatcc agcgttcgga ggatcgtctc
     2641 gaacgtagcc cctccttcgt gattgagcaa tcctcggacg ttctcaagga ataaaagctt
     2701 aggtcggaga agagtggtga accgagcgat ttcaaagaat aaagtacctc gagtatcgga
     2761 aaatctttgc cgctttcccg caattgaaaa agcttggcac ggaaatccgc cgcaaaggat
     2821 gtccactggt ccgagggatt gaataaactc atctgataca cttgtgatgt catgcatttc
     2881 cacctcctta cttgtatcat gaattgcttt ataactacgt ctagcaaatt tatcaatttc
     2941 acaaaaaccg atacaatgat ggctcgcctg ttccatacct aaacgaaagc caccaatacc
     3001 ggcgaataaa tctaaaaatc tcatacatta cctcattcat tgcaaagaaa aaagccaaga
     3061 acatttt
//
LOCUS       DAFBQH010000141         2778 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.148, whole genome shotgun sequence.
ACCESSION   DAFBQH010000141 DAFBQH010000000
VERSION     DAFBQH010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.148"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..197
                     /locus_tag="LK694_002741"
     CDS             <1..197
                     /locus_tag="LK694_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053543.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="LK694_002742"
     CDS             314..811
                     /locus_tag="LK694_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBK5053544.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="LK694_002743"
     CDS             898..1290
                     /locus_tag="LK694_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK5053545.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..>2778
                     /locus_tag="LK694_002744"
     CDS             1274..>2778
                     /locus_tag="LK694_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK5053546.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT          839 a          518 c          596 g          825 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagc
//
LOCUS       DAFBQH010000142         2650 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.149, whole genome shotgun sequence.
ACCESSION   DAFBQH010000142 DAFBQH010000000
VERSION     DAFBQH010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.149"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            36..197
                     /locus_tag="LK694_002745"
     CDS             36..197
                     /locus_tag="LK694_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053547.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
     gene            210..713
                     /locus_tag="LK694_002746"
     CDS             210..713
                     /locus_tag="LK694_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBK5053548.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            773..1162
                     /locus_tag="LK694_002747"
     CDS             773..1162
                     /locus_tag="LK694_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK5053549.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1149..>2650
                     /locus_tag="LK694_002748"
     CDS             1149..>2650
                     /locus_tag="LK694_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK5053550.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKG"
BASE COUNT          788 a          439 c          610 g          813 t
ORIGIN      
        1 aatggttttt aaccaatgcc caatccttag tcttactggc aattgtggtc attggtttgt
       61 ttttaggatt taagcgggag ttttccaaac tgattggctt tttggtgatt gctttgattg
      121 cggtcggatt ggtctttaat gcttctgggg tgaaggatgt cttactcagt ttgtttaacc
      181 gaattgtcgg tgcgtaaggg ggaagcacga tggaacagat gcgtgtttat attgcgaacc
      241 tcgggaaata taacgaaggt gaattggtgg gggcgtggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacgaatt gggttaaatg acgaatacga ggaatatgca attcatgatt
      361 atgaacttcc ctttgagatt gatgagtata cgccaattga ggaaattaat cggttgtgtg
      421 gcttagcgga ggaacttgaa ggaacgccag ttggtgaagt tgcttctgag attcaacatg
      481 ctttcttcaa ttcttttgag gagatggtag agcatgtgga tgatattatc tattatccag
      541 attgcgatga tatgagtgat gtagcatatt tcttgattgt agaagctggt gattttggag
      601 aagtaccaga acacttgaag acgtatattg attttgatgc gtgtggtaga gacttggaga
      661 ttggaggcaa ttatttggta acgagtcgag gtgtttttga gtatccatat taacaaaatt
      721 attgaagggt agttgatttc taaactatcc ttctttttaa aggaatgatt tgatgaagaa
      781 aataaaaagc tacaccagta tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt
      841 tcaactgcct tttcctgtga cttttaacca gatggcttgg tttgttttat ccttgctgtt
      901 tgtcatcgta tttgcccatg tgcctccact atctatgata gagggggcct ttttaaaata
      961 cttaggtata ccggtggcag ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa
     1021 accattggga tttttacgat cttttattag ctatcactta cgatttaagg tcactttcgc
     1081 aggaaagaaa gtcaaagaac agaagaaacg atgggacgaa cccattactc tggtaaggag
     1141 tgtgaactat gtacccgatt aagtatattg aaaataatct tgtctttaat caagagggag
     1201 aatgttttgc ctactatgag ttaatacctt acaattactc ctttttgtca cctgaacaga
     1261 aatatcaagt ccacgataac tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc
     1321 atgccttaca aattgccaca gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg
     1381 aaattaccgg gcgattaaca gaagtagcga aacaacgaat tgatttacag acagaggcat
     1441 tagtatccat gattggcgat agtcagattg attatcgctt ttttattggc ttcaaactga
     1501 ttgcgacaga tgaagaggtc aatctaaaga atctcaagaa atctttcttc tccggctttc
     1561 aagaatttgt ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg
     1621 aagaaattcg ccgatatacc aagcttgaaa aactaatgga aagtaaattg gctcgaagat
     1681 ttaaagtcag acgagtcaca ccgagtgatc tgatgtattt gattgaacat atctatggcg
     1741 aaaagggtac accatttgaa gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg
     1801 aaactttggt gaaacgttac gacttattac gtcccagtcg ttgtttgatt gaagaaaagc
     1861 cccggtactt atgcatggaa catgagaatc atgaatcata tgtagcgtac ttaacaatta
     1921 atacaattgt gggggaaatg gagtttccct cctcagaact tttctattac cagcaacaac
     1981 aatttacatt cccaattgac actagtatga atgtggagat tgtgacgaac aaaaaggcac
     2041 ttgctaccgt tcgcaataag aaaaaggaat taaaagactt ggacaaccat gcctatcaat
     2101 ctgacaatga aacaaattcc aatgtcttag atgcattgga ttccgtagat gaattggaaa
     2161 ctaccttgga tcagtccaaa gaatccatgt acaaactgag ctatgtggtt cgagtaagtg
     2221 ccgaatcagt agatgaacta aagcgccggt gtgatgaagt gctagatttt tatgatgata
     2281 ccaatgtaaa actagtccgg ccttttggcg atatgatggg gctacatgaa gaattcttac
     2341 cttctagtaa gcgatacatg aatgattata tccagtatgt tacgtctgat tttctcgccg
     2401 gacttggttt tggcgccact cagatgttag gggagttgga aggcatttac tttggttata
     2461 acgtagatac ggggcgcaat gtttacctga aaccggcatt ggcctcacaa ggggtcaagg
     2521 gttcagttac caatgcgcta gcagcggcat tcctcggttc actcggtggc ggaaagtcct
     2581 ttagtaacaa tctcttagtt tattacgccg tcctttttgg tggacaagct gtgattgtcg
     2641 atccaaaagg
//
LOCUS       DAFBQH010000143         2642 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.150, whole genome shotgun sequence.
ACCESSION   DAFBQH010000143 DAFBQH010000000
VERSION     DAFBQH010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.150"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            702..2561
                     /gene="ltrA"
                     /locus_tag="LK694_002749"
     CDS             702..2561
                     /gene="ltrA"
                     /locus_tag="LK694_002749"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBK5053551.1"
                     /translation="MNKVKTNKTLRHSEYYGMTQIFDELYVKSDKKEVFTELMTIIMS
                     DENIRLAYRNIKNNKGSYTASTDKKSIRDIKTLSEDTFLTEVKVLFRCYKPRAVRRVE
                     IPKPNGKTRPLGIPSIWDRLIQQCILQVLEPICEAKFHNYSYGFRPNRSTHHAIATCN
                     RLANRGKLHFVVDVDIKGFFDNVNHSKLLRQMWTIGIRDKWLLGIIRSMLNAPIVLPT
                     GKVEHPKKGVPQGGVLSPLLSNIILNELDWWVASQWQNTTIETEKIMDRRDKGKGIDK
                     TNQYRALRRTNLKEIYIVRYADDFKIFCRKRSHADKIFHATKMWLKERLSLEISEEKS
                     KIVNLKKGKSEYLGFELKLIKKGKKYVVESHMSKKSMTRVKIQLKKQLRKVARPKNHC
                     EQAKEIGLYNSMVIGIHQYYGIATCVNLDCSKIAYTLKPFINHKLPTKKHGKILNTFI
                     KSKYGKSKEMRWLNNVPIVPLNYVQFRLAVPLSEGTCKYTESGRSKIHSKLKLDLALL
                     LHMMRNSNYERSIEFVDNRISKYSAQKGKCAITGKFLEYEEIHCHHIVPVKQQGTDKY
                     SNLIIIHKNVHALVHAIEEKIIHKYLNVLNLTNEQIEKLNELRVKAGNSVLTV"
BASE COUNT         1021 a          421 c          505 g          695 t
ORIGIN      
        1 gcacttaaaa gaagaaaaca taagtttcct tttaaggaga actgatattt tgagcagtgg
       61 aatgatgggg taacgccctg aaacgctccc ttaatactcc gactggctgt ttaacatata
      121 gctgttagat ggtcagaagc tcggtgaagt cggctgaaag ttaccgtaaa tatgataaat
      181 tcatatacga aagttgttca gaggtgaatg atgtaaccta taggtcggag gtctataaaa
      241 tactcacggt gagaatgtaa gtaatactac tgacgaactt gcgaatgaaa gaatctatac
      301 acggactgta tagaaatgta caggattcta ccgtatcaac ggaacagaat gtatgttgtg
      361 gtttagtaag attattggtt atgaacaacc atgcaatgtt acaggcattg gaaatataca
      421 ggttcaaagc aagcacctaa aagtatatat aaagataaaa atatcgaaac atgggaagct
      481 ggcaatgtgg aggaaactat attccattga aacatttgtg acgaagaata ataagcacat
      541 ttatgccagt gaaagtggtg gcacagtacc aatgaggata tagaaatata tcggaggaat
      601 agccactagt caaaagaaat cgttaattaa atgatgtatt tcatagaatc gagtaagaca
      661 aaataaacac tacaacctaa gtaaacaaag gtagtggtgc tatgaataaa gtaaaaacaa
      721 ataaaacact tcgtcatagt gaatactatg gcatgacaca aatatttgat gaactgtatg
      781 taaaaagtga taaaaaagaa gtttttacag agctcatgac aattattatg agtgatgaaa
      841 atattcgact agcatacaga aatatcaaga ataacaaggg cagttataca gcgagtaccg
      901 ataagaaaag tattcgagac attaaaacac tgtcagaaga tacctttcta actgaggtta
      961 aagttctttt tcgctgttac aaaccacgag cagttagacg tgtagaaatt cccaaaccaa
     1021 atggaaaaac acgccctctg ggcattccat caatctggga taggttaata caacagtgta
     1081 ttttacaagt cttagaaccg atatgcgaag ctaaatttca caattatagt tatggatttc
     1141 gaccaaaccg ttccacacat catgcgattg ctacctgcaa tcgactggca aataggggga
     1201 agctacactt tgtagtagat gttgatatta aaggattctt tgataatgtc aatcactcta
     1261 aacttttgcg tcaaatgtgg acgattggaa tccgtgataa atggctatta ggtattattc
     1321 gatccatgct aaatgctcct atagtcctac caaccggtaa agtcgaacac cctaaaaaag
     1381 gtgtacctca aggtggtgtc ctatcccctt tactatctaa tatcatattg aatgaattag
     1441 attggtgggt tgcttcacaa tggcaaaata ccacgattga gacagaaaag attatggata
     1501 ggcgagataa aggcaaaggc attgataaga ctaatcagta tcgggcatta agacgaacaa
     1561 acttaaaaga aatatacatt gttcggtatg ctgacgactt caaaatcttt tgtagaaaac
     1621 gaagtcatgc agataaaata tttcacgcaa cgaaaatgtg gcttaaagaa cggttatcac
     1681 ttgaaattag cgaagaaaaa tccaaaattg tcaatctcaa aaaaggtaaa tcagaatatc
     1741 taggatttga attaaagcta atcaagaaag gtaaaaaata cgtagtcgaa agtcatatga
     1801 gcaaaaaatc aatgacaaga gttaaaatcc aattaaagaa acaattacgt aaagttgcca
     1861 gaccgaagaa tcattgcgaa caagcaaaag aaattggatt atataattct atggtgattg
     1921 gaatccatca atattatgga attgcaacct gtgtaaatct cgattgcagt aaaatagcct
     1981 atacactaaa accgttcatc aatcacaaac tgccgacaaa gaaacatgga aagatattaa
     2041 atacatttat caaatcgaag tatggaaaaa gtaaagaaat gcgatggttg aataatgtgc
     2101 caattgtacc gctcaactat gttcagttta gacttgctgt ccccctatca gagggaactt
     2161 gcaagtacac tgaaagtgga cgaagcaaga tacacagtaa attaaaatta gacctagccc
     2221 ttttacttca catgatgaga aatagtaatt atgaaagaag tattgaattt gtggataaca
     2281 gaatatctaa atattctgct caaaagggaa aatgtgctat cacgggtaaa tttctcgaat
     2341 acgaagaaat ccattgccac catatcgtac cagtaaaaca acagggtaca gacaaataca
     2401 gcaacctcat tattattcac aaaaatgttc acgcattagt ccatgctatc gaagaaaaaa
     2461 tcatacacaa atacctgaat gtattaaatc taaccaatga acaaatagaa aaactaaatg
     2521 aattacgtgt caaagctggt aatagtgttt taacagttta acttgaaaaa tatatcatac
     2581 ttaacattct tttgatggaa cgccgtatga agtgaaagtt tcatgtacgg tgtgaagcgg
     2641 gg
//
LOCUS       DAFBQH010000144         2623 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.151, whole genome shotgun sequence.
ACCESSION   DAFBQH010000144 DAFBQH010000000
VERSION     DAFBQH010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.151"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="LK694_002750"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="LK694_002750"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBK5053552.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          941 a          458 c          581 g          643 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaa
//
LOCUS       DAFBQH010000145         2557 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.152, whole genome shotgun sequence.
ACCESSION   DAFBQH010000145 DAFBQH010000000
VERSION     DAFBQH010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.152"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(48..1514)
                     /locus_tag="LK694_002751"
     CDS             complement(48..1514)
                     /locus_tag="LK694_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBK5053553.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1901..2497
                     /locus_tag="LK694_002752"
     CDS             1901..2497
                     /locus_tag="LK694_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBK5053554.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT          884 a          502 c          444 g          727 t
ORIGIN      
        1 aacaggaact gctgcaaata agcaattcct gtttttatgt tcattgatta atgaatagag
       61 cccattagtt ttttgattgg ttctttcaca gtaaatagca agataccacc gacgattgct
      121 actgcaccga taataccgaa gtaagcagat tctgttcccg gtgtgtagaa gcgtgcaatt
      181 tgtgcattga ttgcttgtga agaagcatca gctaagaacc aaatagcgat tgtttgtgat
      241 tcaaatgctc gtggtgctaa ttttgttgtc acagacaaac caactggtga aagacacaat
      301 tcagcaataa ccacgatgaa gaaacttccc aacaaccaca atggacttac ttttccactt
      361 gtaccataaa gcatacccgg caacatcaat agcaagtacg ataatcctgc aaataaaagt
      421 ccaagagaga atttcactac tgtagatggt tgacgttcac caagtttcgt ccacatcgtt
      481 acgaatactg gtgtcagaat aaagatgaaa attggattca atgattgata ccagcttgct
      541 tggatcgtta caccaaatag cgtagattgt gtacgttcac tagcaaataa agctaagata
      601 gatgaacctt gttcttgtaa tgaccagaaa atgattgctg caaggaataa tgggatataa
      661 gcccatactc ttggtttttc ttcagcagtt acatcttttg aagtcagcat tttgataaag
      721 taatacaacg gtaagataat ccccaataca ctgattgtat tgacaaaaaa gtcaattgtc
      781 agatgaccag ttagttgtgc cccgccgaat actgcagcga taacaacaag tgcgatgatc
      841 aagttacgga tgaatccttt acgttcttca gttgtcaaag gattcggcgg tgttgtaccc
      901 actccgtcta atgtcttgcg tccttggatg atgtattgta ataatccaaa gaacatccca
      961 atagcagcta aagagaaccc taaatgatag ttatattctt gaccgattgt accaacgatt
     1021 agtggtgcta ataatgcccc taagttgaca cccatgtaaa aaatcgaaaa tcctgcatct
     1081 cttcgcaagt cagtctttga ataaagatgt ccgaccatgc cagaaacatt tggtttcaaa
     1141 aaggcggttc cgataataat caatgccatc gaaacaaaca atgtacttac accaaatggt
     1201 aaagccaaga cgatgtggcc aagcataatg aaaactccac cgaagaatac agtgcgtttt
     1261 gctcctaaca ctcggtcaga tacccatccg ccgacgatac tagacatgta gatcaaagat
     1321 ccgtagatcg acatgatagc taatgctgtt gctcgtggta atccaagtcc cccgttggca
     1381 actgtatcgt acatataata gataagtaat gcacgcatac cgtaataact aaatcgttcc
     1441 cacatctcag tgaagaacaa agttgacaat cccttcggct gcccgaagaa gcccttgtct
     1501 tgctgttttt ccataatcta taccccaatt ctttaaattt tcagaaaata ttatacatct
     1561 ttaccgaaaa atttaactaa aaaacttatt taagaaatat aaactaattt tctataaaag
     1621 caagtaattt ttaatggcaa tatcataaaa aaaagaagat agagatttaa taattatata
     1681 tcagctgttt ttttcaaata agcaaaaatt cctaaaacac aacaatgaaa gcgataacta
     1741 agaaaatcct tacataacga tttttcttat taaaattgtt ttttgaactt cgcttatttt
     1801 ttgtgagcat atctgtttac ttactttttg taaggtgcta tacttcattt cgtaatcaat
     1861 taaaaaaatg caaaacgcaa atcaaaggag aaattttatt atgacaactt ttactgacac
     1921 tttgaaaaac cgccgttcaa tctatcatct aggacgtaat gtttcacttt ctaatgaaga
     1981 attaacaact ttgatcaaag aagcaattaa agaaagccca actgctttca atgctcaatc
     2041 aacacgcgca gttatccttt tcggagatgc acatgaaaaa ctttgggaaa tcactgaaga
     2101 agcacttcgc ccattgactc cagcagaagc tttcccaaat acacaaaaca aattagctgg
     2161 tttcaaaaat ggttatggta cagtattatt cttcaaagat actgacgtgg taaaaggtct
     2221 acaagaacaa tttgaattat atgcagacaa cttcccagat tggtcagaac aatcaaacgg
     2281 aatcgcaaca gctaatactt gggtagcatt agttgataaa ggattgggcg caaacttgca
     2341 acattacaac ccagttattg acgaagcagt tgcaaaagaa tggaatatcc cttcaaactg
     2401 gaaactacgt tcgcaattag tattcggttc accagaaaca ccggctgggg aaaaagaata
     2461 catgaacgat gccgatcgct tccgcgtatt tggataattt tatttgaaat aaaaaacaga
     2521 ggttgtgaca aacatcttgt cgcaacctct gttttta
//
LOCUS       DAFBQH010000146         2397 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.153, whole genome shotgun sequence.
ACCESSION   DAFBQH010000146 DAFBQH010000000
VERSION     DAFBQH010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.153"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            206..2068
                     /locus_tag="LK694_002753"
     CDS             206..2068
                     /locus_tag="LK694_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK5053555.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2397
                     /locus_tag="LK694_002754"
     CDS             2068..>2397
                     /locus_tag="LK694_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK5053556.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          864 a          353 c          466 g          714 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataat
//
LOCUS       DAFBQH010000147         2349 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.154, whole genome shotgun sequence.
ACCESSION   DAFBQH010000147 DAFBQH010000000
VERSION     DAFBQH010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.154"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            253..1551
                     /locus_tag="LK694_002755"
     CDS             253..1551
                     /locus_tag="LK694_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBK5053557.1"
                     /translation="MKHTHKIILAVTSVCTVIILGVSIFFVTQAATNNKLENTSQTTQ
                     HSSSSSKPVEDKQTTKQLDQAKQLAASYHYDEAIALLEKDDAKEAQQLLATLKKEKES
                     LVKWEDPTKISHVFFHSLIVDPAKAFHTQQAQGYKDYMVTISEFNKTIDQLYKNNYVL
                     VNLNGLVKKGTDGKLTFTGVSLPEGKKPLILSQDDVSYYEYMDNSGFPSKLIVDKQNQ
                     IKNIYIDNKKETVGDYDMVPLIDSFIKKHPDFSYQGAKGTLALTGYNGVLGYRTSKSE
                     YGDNEKTHKEIEAAKKVADQLKEDGWSFASHTWGHLNMTQASLADIQQDNERWQNEVA
                     PILGKTNILIYPFGADISDWQPYSEANQKFAYLKQQGFDIFCNVDASTPAWGQLGTDY
                     YRNARINIDGIRFEADLKGENPILDHFINVKEMYDQKDRG"
     gene            2198..2349
                     /locus_tag="LK694_002756"
                     /pseudo
     CDS             2198..2349
                     /locus_tag="LK694_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          836 a          400 c          429 g          684 t
ORIGIN      
        1 ttttagtttt tcttggaaat aaaaacagcg aaaatccgaa cattttcacc tctctcactt
       61 tataactttt acaaaatagt acttctcata aaaagcgctc tagttaaaga aaataagaga
      121 aaaatcaaaa aaaacaaaag tttcttttac acttgtttct atattcgtta caatggttat
      181 gggaagtttt taaaaaggtt aagaaaagct tacgggggtt tttgaaagca aaaagtagta
      241 ggggacgata aaatgaaaca tacacacaaa atcatattgg ctgtcacttc tgtatgcact
      301 gttattattc taggtgtaag tatctttttt gttacacaag cagcaacaaa caataaatta
      361 gaaaacacta gtcaaacaac tcaacactca agcagcagct caaaacctgt tgaagataaa
      421 caaacgacga aacagcttga tcaagcaaaa cagcttgctg caagttatca ttatgatgaa
      481 gccatcgcct tacttgaaaa agacgacgcc aaagaagcgc aacaattgtt agcaactttg
      541 aaaaaagaaa aagaatcctt ggtaaaatgg gaagacccga ccaaaatctc acacgtcttc
      601 tttcatagtc tgatcgttga tccagctaag gcttttcata cccagcaagc acaagggtat
      661 aaagattata tggtcactat ttccgaattc aataaaacca ttgatcagtt atataaaaat
      721 aactatgttc ttgttaatct aaatggatta gtcaaaaaag gaacagatgg aaaactgacc
      781 tttacaggtg tttctcttcc agaaggaaag aaaccgttaa ttctttcgca agacgatgtc
      841 agctattatg aatacatgga caattcagga tttccaagta agttgattgt cgataaacag
      901 aaccaaatca agaatattta tatcgacaat aagaaggaaa ctgttggtga ctatgatatg
      961 gttccattga tcgattcatt cattaaaaaa catcccgatt tttcttatca aggcgccaaa
     1021 ggaacattag ctttaacagg ctataatggt gtattaggtt accgtacatc aaaatcagaa
     1081 tatggtgata acgaaaaaac acacaaagaa atcgaagcag ccaaaaaagt tgctgatcaa
     1141 ttaaaggaag atggttggag ttttgcttct catacttggg gacacttgaa tatgacacaa
     1201 gcttctttag ccgatatcca acaagataat gaacgttggc aaaatgaagt tgcacctatt
     1261 ttaggcaaaa caaatatttt gatctaccca tttggcgctg acatcagcga ttggcaacct
     1321 tattctgaag caaatcaaaa atttgcttac ttgaaacaac aaggatttga tatcttctgc
     1381 aatgtcgatg cctcaactcc tgcatgggga caactaggaa ctgactacta ccgtaacgcc
     1441 agaatcaata ttgacggtat ccgtttcgaa gctgacctaa aaggagaaaa tccaatactt
     1501 gatcacttta tcaacgtcaa agaaatgtat gaccaaaaag atcgaggata gaggttgtga
     1561 aagaagcgtc cagctccgag caaataagaa ctagatttct aaaatagttt ctcatatttt
     1621 ggaaatctag cgcttattgc tgaggagttg cttcttgaac accgtgaata gaggttgtaa
     1681 aaagagcgtt ttgacttatt gttgaaggtc agctctttga acaccgcgtg ttaggttgtg
     1741 aaaaggctgg tctgcatcaa gcaataagaa tgaacagaga aaaacagctt cttgaatacc
     1801 gcagataggt tgtaaaaaaa ccgttttgac ctgacgataa ccagtaaaac agaggtgaga
     1861 ccattgctaa aagtagcagt ggtctcatct ctttttttgc acaagtcata tttgattgtt
     1921 tcgttatttt aatcaggata aagttctttt tttcatgcta aagagtgatt ctttcttctc
     1981 gattttcact ttcttcgttt aacttttggt aaatacttct gtcagaaaaa gctctaggta
     2041 gacacacgaa agcagataaa aagcctaact tttgcaacag tttatgctgt cgttcaaagt
     2101 taggctttta cagtttataa atagggttct gttgcaaagt tttccaaaaa atctatttta
     2161 gtgtaaaatt gagaaaaaag acagagagga cagagtaatg aatcatttta aaggcaaaca
     2221 attcaaaaaa gacgtcatta ttgtcgctgt tggttactac ctgcgttaca atctaagcta
     2281 tcgttaagtt caggaattgt tatatgatcg tggaataaat gtttgtcata ctacgattta
     2341 tcgttgggt
//
LOCUS       DAFBQH010000148         2340 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.155, whole genome shotgun sequence.
ACCESSION   DAFBQH010000148 DAFBQH010000000
VERSION     DAFBQH010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.155"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(365..1657)
                     /locus_tag="LK694_002757"
     CDS             complement(365..1657)
                     /locus_tag="LK694_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBK5053558.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1931..2191)
                     /locus_tag="LK694_002758"
     CDS             complement(1931..2191)
                     /locus_tag="LK694_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053559.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          781 a          441 c          355 g          763 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
     1681 ttgtagaaaa agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg
     1741 gtttatatta cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt
     1801 atctatttga aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatccta
     1861 ttatccatat ctttttcagt tttaatattg atacacatta taagaaaaag aaacttttgg
     1921 ttaatttatt tcatcccagt gcaaaatata aaaaagtaaa atagaccata ggcaatagat
     1981 taacaattaa tataatacaa gcaaatattc tattcattgt atttctgctt tcaaaaagtt
     2041 ctaatattga aagtataatt ccacaaatgc ctataataag tatgggaata agattagaaa
     2101 ataacacccc gagccaatct gtgaaaatta ctatatttac taatatccct attaacatac
     2161 ctatcatatt tattaaatca atttttttca ttagatattt ctcctttatt aaatctgaag
     2221 tacttatatt aaaataattc taaactattt acctcaaaaa gtgcaataaa ttatttctga
     2281 acaataaaat gatagtcaaa acaaaaaaat attgttaatc gttcagagcc atccgagagt
//
LOCUS       DAFBQH010000149         2327 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.156, whole genome shotgun sequence.
ACCESSION   DAFBQH010000149 DAFBQH010000000
VERSION     DAFBQH010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.156"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            61..624
                     /locus_tag="LK694_002759"
                     /pseudo
     CDS             61..624
                     /locus_tag="LK694_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            630..1046
                     /locus_tag="LK694_002760"
     CDS             630..1046
                     /locus_tag="LK694_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053560.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            1152..1415
                     /locus_tag="LK694_002761"
     CDS             1152..1415
                     /locus_tag="LK694_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBK5053561.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            1415..1687
                     /locus_tag="LK694_002762"
     CDS             1415..1687
                     /locus_tag="LK694_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBK5053562.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            1764..2093
                     /locus_tag="LK694_002763"
     CDS             1764..2093
                     /locus_tag="LK694_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK5053563.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            2083..>2327
                     /locus_tag="LK694_002764"
     CDS             2083..>2327
                     /locus_tag="LK694_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK5053564.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCG"
BASE COUNT          859 a          317 c          460 g          691 t
ORIGIN      
        1 ttttcttcta gtctacacac cttaattttt ctgtctagtt aattgtagcc tatccataga
       61 ataaatgttt gtcatactac gatttatcgt tgggtgcaag agtacagcaa agtcctctat
      121 tatctttgga agaagaaaaa tagacaatcc ttctattcat ggaaaatgga cgaaacctat
      181 atcaaaatta agggacgttg gcattatctt tatcgtgcaa ttgatgcgga cggcttaacc
      241 ttagatatct ggttacgaaa gaaacgggat acgcaagcag cctatgcttt cttaaaacga
      301 ctccataaac agtttggtga gccgaaagca attgtgaccg ataaagcacc ttctcttggc
      361 tccgccttta gaaagttaca gagtgtgggt ttatatacta agacagagca ccgaactgtg
      421 aagtatctta acaatttaat agaacaagac catcgaccta ttaaacgacg gaataaattt
      481 tatcaaagtc tccgtacagc ctcttccacg attaagggca tggagaccat tcgaggaata
      541 tataaaaaga accgaagaaa tggaacgctc ttcagctttt cggtgtctac tgaaatcaag
      601 gtattaatgg gaataacagc ctaagatatt tggagttcag agagggcgcg tttgattttc
      661 aaacttcgca acagaaccga aaatttatac cggaagattt tcaaaactta cagttagaaa
      721 acatgcgttt agaagattta tttagattag cagatgatga taagtataga tttgaagttt
      781 tgaaaattaa aaagaatgaa ctattgaata agttatatcc tgagaatgcg ataaaagcac
      841 attttggtga tgctaatcat atgtatagtt ggttcattcc taaaagattt agagggaaaa
      901 tcaatgctgg agatcaagtg aaagtactca atcgaaatag tacagatgtc gtaatgataa
      961 cagatatcta cgtagaagat gaagttataa tcaaatatat gaagcctgtg ataaaacttg
     1021 ttaataaaaa taaaggaaac ggataacact ttacaatgct gttttggtac tccaatttaa
     1081 acaaaacaat cgaatttacg tacttaatta agtacgttag aatggagata taaattagag
     1141 ggggagtaga tatggcaaaa acagtcttaa atcctagctc agcacggaaa aatttttata
     1201 agttgttgaa agatgtaaat gagaatcata cggaaattga aatcattagt gagcgcagtg
     1261 aaaataatgc ggtgcttatt ggtttggatg atttgaaagc aatcaaagaa acgctattac
     1321 ttgagcaaat aggcactttg ggtgttgttc gtaagcgaga aaaagatgat agtggattta
     1381 ctaatgttga tgaaattgat tgggataact tataatgaat aactattctg tgatgattaa
     1441 aaactcggct aaagctgatt taaagaaaat caaacagtct aatttgaaac ctcaatttga
     1501 gaaggttatt cagactttaa aggaagatcc ctatctccct acccaatcat ttgagaagtt
     1561 aaaacctaca tatgaaggta ggtattctag aagattgaat agacaacacc gggttgttta
     1621 taaggtagat gaagatgaac acgtggttga aatttattca gcaaggacgc attacgagtc
     1681 acagtaatta cttgtaaata cattatctcg tggtataatg tatttactaa gtaattacat
     1741 actgttagaa atgagggatt taaatgatta caaaaacaag aaaacaaggc aattcaatca
     1801 tgttgacagt acctaaagat tttaatgttc ctaatggagt cgaagttgaa gcaaagttgg
     1861 tagagaatgg tattctttat gaatttgttg aaccacaaaa agaattttat gattttagcg
     1921 aagatatttt gtctgatata attgcagaag gctacgataa agatgagatt cttgtagaat
     1981 ttaagaatag aaaaaacaaa atgcattctt cctttagaga tattgctgaa gatacactta
     2041 ctaattctaa agtaatgact aaagaggagt tggcaacaga aattggctta tgaaatcgtt
     2101 ccttcaaaac atgtcattaa gtatttaaaa aagctaaaag agaagccatt gaaagaaaaa
     2161 tttttgaatt taatttatga tgaaattgcg attgatccat ataagggtga acaaaaaact
     2221 ggggatttat caggtatttg gtctagtggg ttcaaatatg cgggcacaac atatagagta
     2281 gcttacgaga ttatagaaaa caaggttatt ccagtattgt tatgtgg
//
LOCUS       DAFBQH010000150         2293 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.157, whole genome shotgun sequence.
ACCESSION   DAFBQH010000150 DAFBQH010000000
VERSION     DAFBQH010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.157"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(51..1598)
                     /locus_tag="LK694_002765"
     CDS             complement(51..1598)
                     /locus_tag="LK694_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBK5053565.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="LK694_002766"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="LK694_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBK5053566.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="LK694_002767"
     CDS             complement(2043..2237)
                     /locus_tag="LK694_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053567.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          644 a          477 c          408 g          764 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agt
//
LOCUS       DAFBQH010000151         2278 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.158, whole genome shotgun sequence.
ACCESSION   DAFBQH010000151 DAFBQH010000000
VERSION     DAFBQH010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.158"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            22..399
                     /locus_tag="LK694_002768"
     CDS             22..399
                     /locus_tag="LK694_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053568.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            1005..1619
                     /locus_tag="LK694_002769"
     CDS             1005..1619
                     /locus_tag="LK694_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK5053569.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          774 a          331 c          430 g          743 t
ORIGIN      
        1 aaaaaaaggt gtgtgttcac tatgggaaaa ttagtcgctg ttgcacagtc gctgcttgat
       61 atactcaagt ggctgatgga aggaagcaat tggatatttg tgttgccact attgcttatt
      121 agtctattta tcatgtttcg catggtcaga aaaaaagaaa cgtttctgat ttttatctgg
      181 tatgcagcat ccgttggggt tgctaccttg atggttgaat tggcgttagg tagttgggaa
      241 gcttgcgtat ttgctttatt ttttgctgta ctgtatctaa tcatttatat cccatatatg
      301 aaaagaaagt ctcggatagg cagaaaaggt agacatacaa ctactttttc tagtacagat
      361 tctctcgaac aaagtagaag aacgagaaga agacggtaat atttagtagg tatacgcaaa
      421 aaaagataaa ataatggata aactattgag atgagagatt tatttaattg gtggaaaaaa
      481 gcttttttca cttatataaa agaatctttt ttaaaggaat gcttgttgta tcgggataag
      541 ccattccttt ttttatgtta tttatatata tataaatcat accaatgttt ttttgtattt
      601 tcttgcttgc acttggataa gagattactt acaataaatt cagatagaaa cagctgaata
      661 aaaaaaggct ccctgaccct tagttggcgc taagaatcag ggaatgacca gtcacacaca
      721 tacaccctga ccgatcattt tgccatagtc aatgcagtgc atcgacctaa tttattgtac
      781 ctaatctagg gtgaaaattc tacccctatg tagatatttt ccatggtctg cacgttgttt
      841 tatttgctat gtcaaggtat tgggcgtatt cgttcaatgc cttttttgtg tttgaaacag
      901 caaagaccac atgtgttact gtctaactac tgttttttta tgtacttcct tagattaggc
      961 agctgtttct attcatgtca agtatagcta ggcgggagag ataaatgagt ttatgttatt
     1021 ttgactatta tcaccaattg gaagagaaat taaggataga agagccgctg atgcttcaag
     1081 ctggcacttg gatcgcgaaa caaattaatc aaggtggtag attgcagata tatgctagcc
     1141 gcacactaag tggtgtagca tttgaatttt gggatcaaac cccggatctt gtaccaagta
     1201 tattgattga aaatccagcc ggaggtgtct atgaaacgct agaaggtgca ggacaggcaa
     1261 ttattgatca gctttctgtc aaatcagaag acatttttct tcttctttcg aatgaaggaa
     1321 gaaacccggc aatcatcgaa ctagctcagt ggatcaaaga aaatggtcat ccattaatca
     1381 ttgttactgg atttgatttg tcacgaagta tcaaatcgca acatagcagt ggtttgcgat
     1441 tatttgaatt tgctgatttg gttttggaca accacgccaa tttacatgat gcagtcttga
     1501 ctttgccaaa tctagatcca gcgatctgcg gaacagcctc tcttgcaacg atctttttgc
     1561 tgcagcaagt tttgtatttt acagcaaaac agctagtcca ggacacaagt ggaaaatagt
     1621 tatccacatg ttgacaaagt tccttaaaaa aataatgttc cacataaaaa tggagcgttt
     1681 gaaaaaaaga aaacgattat taatgaggat taacaacgac tacttttttt gttttagtta
     1741 ttttaagatg gatcatcttt gattttaagc ttgttttgtt taaagtttgt tatttttata
     1801 agttgtttca cagttatgag aaatgagtta taagaatatg tagtgtcaga aaaataaaaa
     1861 gtagtggaaa aagagacaag aaagaatcca tatggattct ttcttgtctc ttaatacacg
     1921 ttgatgaaag agtcttcccc aaaactcttt acaaagtaat tatagtacat ttgttatcat
     1981 tattgcaatg aagtttacct gatataacaa aattgtccaa caggatacct ttttctaata
     2041 gaaataaaat acacaaaaat tgaaggatag ataatggata tttataagtg tttattattt
     2101 tttttgcaat gaaattcaat ttttcaatga ttgtaaaatg aaaaaaacag gacgagattt
     2161 tctctaatct aaaaataaaa aaagaatttt aaggaaaaac agagaaataa aatacttaat
     2221 ataaatggaa agagaaaaaa ataatcatac ataggagaaa aaaaacaaaa tagaaaaa
//
LOCUS       DAFBQH010000152         2276 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.159, whole genome shotgun sequence.
ACCESSION   DAFBQH010000152 DAFBQH010000000
VERSION     DAFBQH010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.159"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            64..246
                     /locus_tag="LK694_002770"
     CDS             64..246
                     /locus_tag="LK694_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053570.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            230..415
                     /locus_tag="LK694_002771"
     CDS             230..415
                     /locus_tag="LK694_002771"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HBK5053571.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            658..2178
                     /locus_tag="LK694_002772"
     CDS             658..2178
                     /locus_tag="LK694_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBK5053572.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
BASE COUNT          781 a          391 c          466 g          638 t
ORIGIN      
        1 atctcgtacg ccgattatct ttcttctata attaaactat caaatataga aggagaaaaa
       61 cgaatgaata aaaaagatat tgtaccagtt caattttcaa gtgcggctca aaaatcgcaa
      121 attgacacca agccaaaact agcttgcaga atcaaatcgg aagataagga aatatttatt
      181 tataatggaa tggataaata tcttttgtat accttgttaa aagagttgaa tgccaatgct
      241 cattgatgca actgcattga aacatatttt tatcgtttgt ggaaaaacag atcttcgaaa
      301 aggaattgat ggactagcag ctttcgttat agaagaatat gaccttgatg tttacgatca
      361 agcgcttttc ttattttgcg gtacgaagaa agatcgcttc aaggctcttt actaggatca
      421 aaatggattt gtattaatgt acaaaagatt tgaaagtggg taccttcaat ggccgcgttc
      481 cagaaaagaa gtcttactga ctggcctttc aatcgaagaa aaatgtacgc taaaagaaac
      541 gaaaaaaggt gcgatatatt agacgaaaac cttgttaaat caaggatttt gtctgttttt
      601 cgttgtcata aaatggtata atagatgtaa gaaaatcgct agaatggtgg tgtaaaaatg
      661 acaaaaatgg aatctgaact tcttgtacaa atcaagcaat taacacagtt gcttgaaatc
      721 cagcaacagg aaaatactct tcttagagaa caaattgctg aaatgaatcg acgcttgttt
      781 ggacgaaaaa aagaaacacc acccgtagat gggcaaatcg atttattaga cgactcaact
      841 tttaatgaac cagagcacac tggacaagaa agccaggaac ccattacggt atcttctttt
      901 aaacgcagaa aacgcaaggg actcaaagca ctttcattag aaggtctgcc tgaagttcct
      961 attcattatg aattaaaagg tgccgattgt ttgtgtgaag actgtggtca tactttacat
     1021 gatatcggtg cgacaaggct tagagaagag ccattgtatg ttcctgccca tatagaaaaa
     1081 caagtcatct accaacatgc ctatcattgc agagaatgtg aagcattagg agaaacaaaa
     1141 atcaaaaaag cgcctgttcc taaaccgttg atcaataata gtctaggttc ctcttcaatt
     1201 gtgacacaga gtatttggga aaaatttgtt aaaaaggtgc cggcatatcg acaagaaaaa
     1261 gaatggcatg cacttggttt ccctcttgat cgtaaaaaaa tcactaattg gcatatcaag
     1321 gtaagtgaaa tggttttaga gccaatctac aattgccttc atcaagaatt aagcaatcag
     1381 gaggtcttac acgcggatga gacaagctat cgggtattga atactcataa agaaaagacc
     1441 ttttactggt tatatgcttc aggtaaaagc gaagaaaaac aagttgtgct ctacgagcac
     1501 gcaaattcgc gttccgttga tgagccaaaa agttttttag aatcatttag cggtttcttg
     1561 catactgatg gttacgcggc ctatggaaag ctgccggtaa acaatgttta ttgttgggcg
     1621 catgttcgca gaaagttttt tgaagcattg ggtaaacaac cgtccccaaa aagtcatgcc
     1681 aaaataggat tagacttctg tgacaagatg tttcgaatcg aacggacctt taataagctt
     1741 agcgcagaag aaaggtttgt agcgaggaag gaaacgttgc agcctgtgat gactgaattt
     1801 tttcattggt gtgagagttt tgcttatttg ccaaaatcgc agcttggaaa ggcggttcaa
     1861 tacgcagtca atcataaaga cggacttcag attgtgttgc ttgatggacg cttggaactt
     1921 tcaaataacc gagcagagcg tgccatcaaa gagttagtga tcggtcgaaa aaactggcta
     1981 ttttctaaaa gcttaaaagg cgctagatca aatggaatta tattgagtat tatccagaca
     2041 gctgttgcga atgggttaaa catccgcaaa tatttaaatc atctttttac agagattccc
     2101 aatctgtctt cgatgacacc tgaagcgcta cgcgcttatt tgccatggaa tcagcagata
     2161 caagaaatct gtaaataata taagccacaa ataacctgat ttgagtgtat caaatcgggc
     2221 tatttgtgtt taggtaccat tctatgacac gcttacgctc aaaactactg aattat
//
LOCUS       DAFBQH010000153         2240 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.160, whole genome shotgun sequence.
ACCESSION   DAFBQH010000153 DAFBQH010000000
VERSION     DAFBQH010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.160"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            153..326
                     /locus_tag="LK694_002773"
     CDS             153..326
                     /locus_tag="LK694_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053573.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            966..1439
                     /locus_tag="LK694_002774"
     CDS             966..1439
                     /locus_tag="LK694_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBK5053574.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1448..1675
                     /locus_tag="LK694_002775"
     CDS             1448..1675
                     /locus_tag="LK694_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5053575.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          728 a          356 c          329 g          827 t
ORIGIN      
        1 tatttattta ttaaattttt tcggagaatt aaaaaataca ataaataact ttagttactt
       61 taatgcgttc ttactttatt tttatattat catttttctt attcacttct ttggtttata
      121 ctattcttat aaataaaaac gaggtatgtc ctatgatgaa aagattgtac tattcattga
      181 tcataacgat tggctatttg attgtatcaa atctaggaaa tatggtattt ggtatcagta
      241 aagaattcag ttggactact acattatggg aatctctatt ctttttcatc ttcgtatttt
      301 tgttacaaaa ttatcgaaaa aaataaacat gtgccatcta tctgttggca catgttttag
      361 gatttatggt actttttatt cggcaatcca ttttattttt ctacttcttc ttaatacctc
      421 taacactttt gatttataaa ctaatccaat gcatctactt gcgcttatta agctatttca
      481 gctagatagt accaccccat gcgtcaatgc ttgattttcc ttttgtataa cattgtataa
      541 gcaataaagg acaaataagt ggtgattaca ccaaaccatt gaataagaaa aattctgata
      601 cgtaaataaa catgaacttg aaaaaattaa tgaatcggtt agctactacg tagttgtctt
      661 ttttctcata cagttctgtt aacttacact tctcttcgct agataataaa aaatcctcca
      721 actgtattgt ccaatataat cccaggcatc taaactagca ttatagaaga gacacacata
      781 gaatctaaaa taactggttt taaatgtttc ttacaatcaa aaaaagctgt taaatgcact
      841 ccacaccttc atactctatc attttttata aacgatatat ttttattgtt tttcgataaa
      901 aaatagttgt tttacaataa ttattctgct atattttagc tatcaaagat aaaagaggga
      961 taaaaatgaa atttaaaaca ttgctattaa aatttgtcgt tttaggtata agtgccgtat
     1021 ttattttgtt cggcgtactt ttttatactc agatcgctac aagcgaaaaa ggattcaatt
     1081 ttgattggca gaccacatta tttgtttctg tcatttactt tgcccttctc ctagggatcg
     1141 ttattgctta tttcttattg aaactattga aaaaaatcga agataaccaa gctttttcca
     1201 ctcaaagctt aaacatacta aaaaaaatcg aacgaaccat tttgtttatt ggtttcgcct
     1261 tttttggctt acttcctaaa tttatgaaag tagcggattt agacgatgct ccaggtttga
     1321 tgttggtagg aatcgctgtc gtattcattc cttttgcagt atacacatta acaagcgtgc
     1381 tggaaaaact gcttgaacat gcgattttac ttaagacaga tcatgatttg acagtgtgag
     1441 gtgcgacatg gcaattatcg ttaatttaga tgtagtatta gctcaaaaaa aaatgagtgt
     1501 gactgaattg agtgagaaag tgggcattac gatggcaaac atctctattc taaaaaatgg
     1561 aaaagcaaaa gccatccgtt tttccactct cgaaaaaatc tgccaagttt tggagtgtca
     1621 accaggtgat ttgttgacct ataaaaatga ctcgaatact ataccagaag aataaaataa
     1681 agtagaacaa acagtaaata aaaaataatc tgaatgatag atgtgtgaga cttttagaag
     1741 ttatttacgt ctattattca gattttttat ttcgattgaa taccgtttta tctataaata
     1801 agatatgtac tgactcatac aaagggggcc ttttcttctc catgtactat cacttttcct
     1861 gatacaactg gtttcctgtt acgttgtagt tggatctgct tttagtataa tactttattc
     1921 attccaccag actgatagca gtttgagcag tctaaataat catgtagtat tacaggccat
     1981 tctgtttcat accttgaagt gcttaaaact tcagctaatg gcccattgtg ttaactctta
     2041 ctccattctt ttttagtaag ctcccatata tactgtaaag ccttctatat attatgtcat
     2101 gttgcttttt aacatttact atgtagtttt gataaaatga tttgaataac agtgatggac
     2161 aattcttttt tatttttaac attattttga taataatgac agactgtaga caaaagctgt
     2221 tccaaactcg taactcgagc
//
LOCUS       DAFBQH010000154         2193 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.161, whole genome shotgun sequence.
ACCESSION   DAFBQH010000154 DAFBQH010000000
VERSION     DAFBQH010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.161"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(562..765)
                     /locus_tag="LK694_002776"
     CDS             complement(562..765)
                     /locus_tag="LK694_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053576.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(1224..2189)
                     /locus_tag="LK694_002777"
     CDS             complement(1224..2189)
                     /locus_tag="LK694_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBK5053577.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          630 a          506 c          346 g          711 t
ORIGIN      
        1 attttgttcc tcctcaaaga aaaagagact tttggaaaga atttccagag ttcacgtaac
       61 cctaaaaatt catatccaaa aatctctagc ttgaagaaac ctaaaaatat tcgtataatg
      121 aacctgagtt acggagctag gtgaagtagt ttccgtaaat agtcgagagt gttagcgcac
      181 tgctcggctt ttattttgct cttttttctc gttttctttg attatagcac tcctcctaaa
      241 aaaataaaaa gacctcaggg aatttcattc cccgaacccc ttgagcctcc tcctgccgaa
      301 atggaaaggg cgcacccgct gtttatttca ttcagctatc ggctttcaca atctaacacg
      361 atcgttccgc taccagtcgc cttacaggct ccattttcgc tcctctaacg tgttttaacg
      421 attgtgccga taactaaacg aaataaacgc taaaaccgct cagaacgcaa aatatgccat
      481 tttagagcta ttgtagaaaa tgtagtttga aatgcaattt tcttctttca gctttttatt
      541 tcccacttgt aaatctgcta attattttct accagacaaa ccattgattt tttggctatc
      601 agttaactca cgattattat gagaaatgct tgtattcttt gttccctttt tgaacgaaat
      661 actaaaccct aaggtgaacc cctccttcgc gctgtcgcta ataaaaagag tgtaaacctt
      721 tctgttactc tttgacaaag agtaacattt tcctaattgt gacatggctg tcacaattcc
      781 aaacgctctc cgagggtttt cgctgtcaca agtgacaacg aaaaaaggag ccgaaagcgt
      841 gtcacgctct caactccttt tacgctttat tttgcttcac gcaaaataat cgcttatctt
      901 aaacattgac gtaaatttac tctcacttat ctgacttttt tactcggtat cactccgagt
      961 aagccgtcat attcattggg ataatctcgt ctgcttcgct ctaattttcc agagtgaggg
     1021 gtaactcctt cccccgaatt accccaaaga gtgacactgg gatatcccta aatttattat
     1081 aaaaagccaa gtctctttgc gcaactgttt tatccaagtc ggcatttgcg gattcgttag
     1141 cgttttttcg ctcttgctgc aaacgcacga cccaccatgc cgacttgtgc tgaaaataaa
     1201 aaagtggaca aaaattcatc cactcattct tccgtcacgt ccaaccccaa aagccacgta
     1261 tccaattctt ttttcaaaac atctgccgat tccgaaaatg tctctcgtaa atgattaact
     1321 aactcatcac tttggctttc ttttttcaac aaatttcgat atttatattt tgtatgacga
     1381 gccaagccag cccataaatt ttgatctgaa atcaatcctg caatcataat tctatcagta
     1441 tgctttgtat cagtcggcaa aaaatgagga cttgcgaaac tgtctaaact ttcatgaacc
     1501 atcgcatacc aatcagtagc ttttccacgc cgtaactgca attccagtcg ccaccaagtt
     1561 tttatttccg gtggtacaat tttccgtttg gtttcttgtt ccaatttttt gttgtacatc
     1621 cttatttgtc gctcactggc acgactaccc caatatgccg tttccagctt cccagaccgc
     1681 ccatagattg gtttgaatga aacagggtca accacacgat attgcgtaat aaaatcatca
     1741 ggcacatcga taatgtcgca agcaatatca gcacgtgaga aatgtggttc taaaaataaa
     1801 tcatggataa agttcttcat cgaacccgcc aaaaattggc taattttatt tgggttaaag
     1861 tcaattcgcc cttgcccttc ttgatatttc aaaatttcaa tgtaagcaat attctcatca
     1921 tgcttatcca caatttgcca accatttaga ccaacagatt taacataacc attattttca
     1981 ttcagtcgca ttaactgttc aaagtctaaa atcaatacat cattttgcgt gtgataataa
     2041 atattctctt tgagttttcc aacaatggta attcggtcaa ttgaccactg tattttaagg
     2101 ggggtggtca aagtttgcac acttaaccgc ctgttagata gggctgttaa attttcccac
     2161 tcatttatga tcgtgccacg tttactcaaa ttt
//
LOCUS       DAFBQH010000155         2190 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.162, whole genome shotgun sequence.
ACCESSION   DAFBQH010000155 DAFBQH010000000
VERSION     DAFBQH010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.162"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            353..1081
                     /locus_tag="LK694_002778"
     CDS             353..1081
                     /locus_tag="LK694_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBK5053578.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNRSIILNDP
                     NWCKQANLREISMPSAWEETKGSKDVKVAVIDSGIDYQHEDLSGNVDESLGYDFFDDD
                     TDPMDTMGHGHILPGLLVLKQTTN"
     gene            1111..>2190
                     /locus_tag="LK694_002779"
     CDS             1111..>2190
                     /locus_tag="LK694_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBK5053579.1"
                     /translation="MVPIRVFGSSGGTSLAILTEAINYASQKGFPIINFSIGSVVYSS
                     TEKAIKNYPGLFVAAAGNNGIDMTDKPFYPASLDIPNIITVGNSSGYERSSDSNYRKT
                     GVDLFARGFRVFSTFLGSKYTYMSGTSMATPHVTGSAALALSLDPDLSTDELKKIILN
                     SVDSFYELSDLCLTGGRLNTNNVVRQVTGKLTASNITLRLNSEWSDEIAKEMCHVHWI
                     DQSGNDLTDQVVVLKNEVDTSNFGDYEVVFASPDLTDQISVTVTVPRLRKLTLGKETA
                     TIDIGSAWDSVIAIDTFQVKAIDDFGNDVTNQVQVTINADIDTINSIQAVIKFEVPEL
                     GLAQIGRLNITAKNSTINGLNEVSIE"
BASE COUNT          769 a          289 c          470 g          662 t
ORIGIN      
        1 cgtggagaaa taataagaac taccagtcag ataaaacaag ataccgaaga ctcactgcgc
       61 aaagggtggg gaatgaataa taaagttaga taataatcaa agaagaacta agtaactacc
      121 tttaaacggt agttacttag ttcttctttg atttgcgtga aatagagggg atttattttt
      181 ctggttacgt gattaaaaaa tccaaaaaat gagcatagga acggttatga ttttagggtg
      241 tgttttaatg agatattgtg atttcattaa caagtgtatg ataatctgtt gttgagttag
      301 taaataagta atagagctaa ctctaaaata aaactattag gaggagatgt aggtgaaaaa
      361 gtttaaaaag aaaatatatt taggattggt tgttggggtg agtatgtttg ggttcatggc
      421 tcatactaaa caagtaactg cagatactaa ccaaaaagaa aggattatga gccaattaga
      481 tgactctcaa atagagtatg tacccaatga gctaattgtc agtttaaagg ctacacaaag
      541 aatttcaaca gagaaaaata aaccgcaaaa tttattgggt gtaaaagtta tggtggaaaa
      601 agatttgact cttaggacaa atgaaagcga ttataataag cgagatcaag tattattagt
      661 gaaattgaac gaagaaatta gtgtggaaga ggcagtaaga acatttaatg cttctgatat
      721 ggtcaattat gcagagccaa actatgtggt tgaactagag ccgttgaatg atgttgagga
      781 gccaaataca actgattctt taggaaatcg atcaattatt cttaacgatc ctaattggtg
      841 taaacaagct aatttaagag agattagcat gccatccgct tgggaagaaa ccaagggttc
      901 taaggatgtg aaggtagcgg tcattgattc aggaattgat tatcaacatg aagatctttc
      961 tggaaatgta gatgaaagtt tgggatacga tttttttgac gatgatacag atcctatgga
     1021 tactatggga catggacaca tattgccggg attattggtg ctcaaacaaa caacgaatta
     1081 ggtgttgctg gaataaattg ggatgttacg atggtaccta tacgtgtatt tggtagtagt
     1141 ggaggtacca gtttggcaat cttaactgaa gctattaatt atgcatccca aaaagggttt
     1201 ccaattatca attttagtat aggtagtgtt gtttatagca gtacagaaaa agcaattaaa
     1261 aactatcctg gattattcgt agcagcagct ggaaataatg ggatagatat gactgataaa
     1321 cctttttacc cagcttcttt ggatatacct aatattatta ctgtaggaaa ttctagtggt
     1381 tatgaacgaa gttctgattc aaattatagg aaaacaggtg ttgatttatt cgctagaggg
     1441 tttcgtgttt ttagtacatt tctaggaagt aaatacacgt atatgtcagg tacctcgatg
     1501 gcaacacctc atgtcactgg tagtgctgca ttagcgttgt cactagatcc tgatctatct
     1561 actgatgaat taaaaaaaat cattttaaat agtgttgatt cattttatga actatctgat
     1621 ttatgtctta cagggggaag actaaatacg aataatgtcg tacgacaagt aacgggtaaa
     1681 cttactgcat ctaatatcac attaagatta aatagtgaat ggtcagatga aattgctaaa
     1741 gagatgtgtc acgttcactg gattgaccag tcaggaaatg atttgacgga tcaagtggtt
     1801 gttctaaaaa acgaagtgga tacatctaac tttggcgatt acgaagtagt ttttgcatca
     1861 cctgatttaa cagatcaaat ctcagttaca gtgaccgtac ctcgattacg taaacttacg
     1921 ctaggaaaag aaacagcaac cattgatatt ggttccgcat gggatagtgt catagccatt
     1981 gatacttttc aagtgaaagc tatagatgat tttgggaatg atgttacaaa tcaagtacag
     2041 gttactatta atgctgatat agatacaatt aattcaattc aagcagtgat taagtttgaa
     2101 gttccagaat taggcttagc ccaaattggt agactgaata ttactgcaaa aaatagtaca
     2161 atcaatggat tgaatgaagt tagcatcgag
//
LOCUS       DAFBQH010000156         2126 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.163, whole genome shotgun sequence.
ACCESSION   DAFBQH010000156 DAFBQH010000000
VERSION     DAFBQH010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.163"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..93
                     /locus_tag="LK694_002780"
     CDS             <1..93
                     /locus_tag="LK694_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK5053580.1"
                     /translation="NHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(959..1489)
                     /locus_tag="LK694_002781"
     CDS             complement(959..1489)
                     /locus_tag="LK694_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBK5053581.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1486..2103)
                     /locus_tag="LK694_002782"
     CDS             complement(1486..2103)
                     /locus_tag="LK694_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBK5053582.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
BASE COUNT          699 a          408 c          237 g          782 t
ORIGIN      
        1 aatcatattc caagaaaatc attgaattac agaacaccaa tcgaaatatt tttgagctat
       61 gtacaagaag cattttattc tagcttaatt tgacaaatca tataatttaa aattaacatt
      121 gattctccta tgaaaatatc ttttaccttt ctcctgtgaa aaggaaattt ccttttttat
      181 agtgttattg atataatcag aatattcatc aaactctgta tctggagtta aaaatctaca
      241 gtaacatcta taagctttgc tagtaatctt cttgggttct ctctccattg tcaagcaaca
      301 tcaaaacacc tacttactat tcgattcttt tctgccaaaa acgagtcagc atcactcaca
      361 acttatgtgt tgctgactcc gactagtcct ttgttcaata tcaacttatt aaacagatat
      421 aatatttttc tttatttcgt atactccaag acttccatct ttctttcatc ttatctctga
      481 aaaaaacaca gcaaagtgcc cttaataaat atacattaga atttgcaaat tttttcatct
      541 taagatatat cctattcata ttctttacaa tcctaatggg tgattacaaa tctatttagt
      601 tcaagttcta cattataaac acctttcttg atagtatcat ctgctgcaca agcgatgtcg
      661 atccacttat taggggattt acgtgctcaa aagtggctat tccttttggg aagtccatcc
      721 ataataatgg tccatggatt tttttagggt cttgtcgttc aaatttctca aaaactttat
      781 tatacttcca tagttttata attatttggt ttcactttgg ctcgttcaaa caccaacttg
      841 tatttctttt caacgttcga actggagccc cccctataat ctcattatta ttttcatgac
      901 tttttactac cgtggaatta caagcaacaa caatattatc tgctaaatga acacctttct
      961 atatcacgct ttttgctcca atccaacaat gtttacctat aactatttta ttatttttat
     1021 tcaaaatcct atgagtattc aattcaaaaa tcgtatgtga atcggtatcc ataaataatg
     1081 tatcccacga tactgtacag ccatctttga attgaatttc cttaaagcaa attatactca
     1141 tttttcctgt cgaattaaac tttgacccaa tatctaaaac tgcatttttc cttatgtcaa
     1201 tatgagcccc ttgtgcgata aacgctggcc cttgtattct tagtattcct tcaatactta
     1261 taatcgtttc ttcatttccc gcagtattta atactggaaa gcccaaataa actatttttc
     1321 cagattgtaa tactatctca attttccctc tcgatttgcg tgtaatccgt actttacgat
     1381 gcaccaaaat aggtaatctc cacgcatctg aaatatgaag aaatttataa ttgaagtata
     1441 tcgttttagg taaatttaat atattcttta ttatcaatcc gagtctcatc tttcaccatc
     1501 tctttttgta atcaaaatta gtttataaag gattacaccc aatataccgc ccaacgtatt
     1561 atatacaaaa tccgaaattt gaaacgttcc tagcctcaaa aaaagttgta aaaattcaat
     1621 tattgctgaa aaataaaatg caatttttcc tccatatact attgaggacc atattttggg
     1681 tttctttata ttttcatatt taatagacat taccatataa acatatggaa taaaaagaat
     1741 aatattttct aaacacgctg tagataactc ttttgttcca gttgttgtat tatacttata
     1801 aatccaccag tctcccataa catcagataa aggatttaac caaattgttc gattgattaa
     1861 tgttgtaaat aaaatcatcg cagtatagaa tatcaaagca caaacttttc tatattttct
     1921 atccttcttt aaatgaagat aaagttccat tagtatacgt ttatagccct cacctgcagc
     1981 aatgccatct gttgcataca taaaatagac cagtcctaaa attgtcaata aaacagcaaa
     2041 cccaaagggt tcatataatt tatataatat atctgtaata atttctgcaa tcactattat
     2101 cataattatt tctccaatat caagta
//
LOCUS       DAFBQH010000157         2104 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.164, whole genome shotgun sequence.
ACCESSION   DAFBQH010000157 DAFBQH010000000
VERSION     DAFBQH010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.164"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            156..374
                     /locus_tag="LK694_002783"
                     /pseudo
     CDS             156..374
                     /locus_tag="LK694_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            869..1468
                     /locus_tag="LK694_002784"
     CDS             869..1468
                     /locus_tag="LK694_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK5053583.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            1532..>2104
                     /locus_tag="LK694_002785"
     CDS             1532..>2104
                     /locus_tag="LK694_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage abortive infection AbiH family
                     protein"
                     /protein_id="HBK5053584.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT          758 a          326 c          392 g          628 t
ORIGIN      
        1 tttaatttaa tattttgcct caaattagcc acacttttta tagaactaag aggaaaaatg
       61 aagttttctt cctattaata tagttgtttg aaatcttaaa tatagcgttt tacgatgtaa
      121 tgggaagtat ggtgaataaa tcaactatgt tcgtacgaag catttactca actaatttaa
      181 tcgaatcttt caataaacaa atcaagaaat acagccaccg caaggaacag ttccaaaatg
      241 aagagtcgat ggaacgtttc ttagtctcgt cttttgatac ttacaaccaa aaattcctag
      301 gtcgcagtca taaaggtttc caacaggccg aaggcgagct cgaacaaatg ctgagtcaac
      361 cgatggagaa ttagaaacaa tctacagtag ggaagaacca ttgacacaaa attattgacg
      421 ctcccaacta aaagtgtcaa aataatgatg aaatcattta gttcttataa attagaaaag
      481 agaaaataaa tattccatat ctactttatt ggatccgtaa catgactaaa ttctgaatac
      541 tttttaaagc ttatcatttg gtaaaactga atataagtta attatataga ctgaaaattt
      601 tgaattttca gaatagaata acttaaaagt actgacaaaa ttttttaaag gggcaatatt
      661 aatgttctga gataatcaaa tcctaccgaa aatatcaaaa atccaatgtg acacaatcag
      721 attgtgtctc attcgaggga atcacacaat cccacgtggc tctaggcaga attgaatttt
      781 tgcttggggc tttttcctta tattgatttt gaatgagact tattatatgt tataatgcaa
      841 ttaagtatca ttggagaggg cgaagaaaat gaagacggta gaacctattc gagacaaaaa
      901 aaagatcgat gcgatgaagg caatcttggc ttcgggaaaa tatggccaac gaaatttggt
      961 tcttttttcg attgggatca acaccgccta tcgcatttct gatttgcgtc aattgaagct
     1021 ggccgacgtg ctggaaattt cacgcggtcg cgtggtggtc aaagatcgat tagcgatgaa
     1081 ggaacaaaaa acagctaaac ataattcggt ctatatttcc aacaaattgc gaaaaacgat
     1141 tttggagtac gttcaacagg aatttcctga tcaactcctt cagcaagatt ttgacggtta
     1201 tttgttccct agtcgaaaag ggacgaatga accgctcaac cgccaaagtc tgtggcgaat
     1261 cattcatgat gctggcacgg caatcggatt gaaagacatt ggcccacatt ccatgcgaaa
     1321 aacgttcggt tactttctct ataaacaggg gaccaaaaca gaaatcattc agtccttgtt
     1381 gaaccattct tctcagcgcg aaacattgcg gtatattggt attacacaag aagacaaaga
     1441 tacagctgta aaaagcttag atttataata taaaagttaa gtttaaacat ggatagtttt
     1501 aaattagtag attgagtgtt gggaggaaag aatgagtaca ttatatatta taggaaacgg
     1561 ttttgattta agtcatggtt taaaaactag atacactgat ttttcaaaat gggttgaaaa
     1621 aagtcatagt gaaatattcg ataaaataga cacaatgata aatgaaaata atgaggtgag
     1681 aagtacagct gatgataaga taggttggtt taattttgaa agtagtttgc atgagttaat
     1741 tcaaagtcca gtctttgaag attatgtaga agagtctaag gattatctca taggttatgg
     1801 acatgaagat tggtcagatt ctgcccatca tgattttcaa aatatggttt atgattatgt
     1861 tgtttctcta gaagaattac aacaactttt caaggaatgg atacgcaaag aaatacagcc
     1921 gacaatagaa cataaaattc caaaatacca attttcgtcc gatgtactat tctttacatt
     1981 caattataca gatgttttgg aaattctata caagcaaccg aaagaaaaca tatatcatat
     2041 tcatggcagt tatgacgata taataatcgg ccactccgag aattttgaca actttatagg
     2101 agat
//
LOCUS       DAFBQH010000158         1836 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.165, whole genome shotgun sequence.
ACCESSION   DAFBQH010000158 DAFBQH010000000
VERSION     DAFBQH010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.165"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..1647
                     /locus_tag="LK694_002786"
     rRNA            <1..1647
                     /locus_tag="LK694_002786"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1742..>1836
                     /gene="rrf"
                     /locus_tag="LK694_002787"
     rRNA            1742..>1836
                     /gene="rrf"
                     /locus_tag="LK694_002787"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          507 a          386 c          550 g          393 t
ORIGIN      
        1 ctggagcgct tagaagtgag aatgccggta tgagtagcga aagacaggtg agaatcctgt
       61 ccaccgaatg actaaggttt cctggggaag gctcgtccgc ccagggttag tcgggaccta
      121 agccgaggcc gacaggcgta ggcgatggat aacaggttga tattcctgta cccgttgttt
      181 ttgtttgagc aatggaggga cgcaggaggc taaggaatgc agacgatcgg aaatgtctgt
      241 ccaagcagta agtctgaaga ggagtcaaat gcttcttttc ttaaggacaa gctgtgatgg
      301 ggagggaaat aatagtaccg aagttcctga tgtcacactg ccgagaaaag cttctagtga
      361 gaaaacagcg gcccgtaccg caaaccgaca caggtagtcg aggagagaat cctaaggtga
      421 gcgagagaac tctcgttaag gaactcggca aaatgacccc gtaacttcgg gagaaggggt
      481 gctgatcata cgatcagccg cagtgaatag gcccaagcga ctgtttatca aaaacacagg
      541 tctctgcaaa atcgtaagat gaagtatagg ggctgacgcc tgcccggtgc tggaaggtta
      601 agaggagtgc ttagcgcaag cgaaggtacg aattgaagcc ccagtaaacg gcggccgtaa
      661 ctataacggt cctaaggtag cgaaattcct tgtcgggtaa gttccgaccc gcacgaaagg
      721 cgtaacgatt tgggcactgt ctcaacgaga gactcggtga aattttagta cctgtgaaga
      781 tgcaggttac ccgcgacagg acggaaagac cccatggagc tttactgtag tttgatattg
      841 agtgtctgta ccgcatgtac aggataggta ggagccgtag aaatcggaac gctagtttcg
      901 atggaggcgc tggtgggata ctacccctgc gttatggcca ctctaacccg caccactaat
      961 cgtggtggga gacagtgtca gatgggcagt ttgactgggg cggtcgcctc ctaaaaggta
     1021 acggaggcgc ccaaaggttc cctcagaatg gttggaaatc attcgaagag tgtaaaggca
     1081 gaagggagct tgactgcgag accaacaagt cgagcaggga cgaaagtcgg gcttagtgat
     1141 ccggtggttc cgcatggaag ggccatcgct caacggataa aagctaccct ggggataaca
     1201 ggcttatctc ccccaagagt ccacatcgac ggggaggttt ggcacctcga tgtcggctcg
     1261 tcgcatcctg gggctgtagt cggtcccaag ggttgggctg ttcgcccatt aaagcggcac
     1321 gcgagctggg ttcagaacgt cgtgagacag ttcggtccct atccgtcgcg ggcgttggaa
     1381 atttgagagg agctgtcctt agtacgagag gaccgggatg gacttaccgc tggtgtacca
     1441 gttgttctgc caagggcatt gctgggtagc tatgtaggga agggataaac gctgaaagca
     1501 tctaagtgtg aagcccacct caagatgaga tttcccattt ctttaagaaa gtaagatccc
     1561 tgagagatga tcaggtagat aggtcaggag tggaagtaca gtgatgtatg gagcggactg
     1621 atactaatcg atcgaggact taaccaatag gttaaaagaa aagaactcgg aaaaaaactt
     1681 atgtgacttc aaatccagtt ttgagtgaac aaaatttact caataaaaga taacaccaca
     1741 gtgtggtggc gatagcgaga aggatacacc tgtaaccatg ccgaacacag aagttaagct
     1801 tcttagcgcc gattgtagtg aggggttgcc ccttgt
//
LOCUS       DAFBQH010000159         1808 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.166, whole genome shotgun sequence.
ACCESSION   DAFBQH010000159 DAFBQH010000000
VERSION     DAFBQH010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.166"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..242
                     /locus_tag="LK694_002788"
     CDS             <1..242
                     /locus_tag="LK694_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053585.1"
                     /translation="LPKGDKGDPGINATTTAVATTTANGLMSSADKTKLDGIAAGAQK
                     NPGNATTTTAGLMSATDKAKLDGLANITFEKVGTV"
     gene            242..766
                     /locus_tag="LK694_002789"
     CDS             242..766
                     /locus_tag="LK694_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053586.1"
                     /translation="MADIVQLKEKGVPKYLKTHAKGIDGVEGVLVKATGNETVLGTKN
                     FKDGLQFNGLPVQAGMIERAITLADRSDTTNVTDVNGKIIRIGNIVFLTFNFKCGTWP
                     EGSETRWILRIPDGYKRDQGYPAQTALSLVRNASQPADARAFIDQSSIIQAKSGSGSS
                     YISGMWITRDPWPA"
     gene            794..1201
                     /locus_tag="LK694_002790"
     CDS             794..1201
                     /locus_tag="LK694_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053587.1"
                     /translation="MKTIYKVLYPLGYEEHEVEDNFPTGLPFVEVPPILFEKKEDETD
                     EAFGRRQQSQFFNFTENKWEEAVTQDYSKKLELLENLSIGLQVDNAALKKANEELTTK
                     AEGLAQINSKTMLTSLQNTKDIATIKEQLDGGK"
     gene            1203..1340
                     /locus_tag="LK694_002791"
     CDS             1203..1340
                     /locus_tag="LK694_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBK5053588.1"
                     /translation="MYSYDDIKMMFDWNCFTADQVRQFVPLCITDEEAEKIINNEESA
                     S"
     gene            1378..1671
                     /locus_tag="LK694_002792"
     CDS             1378..1671
                     /locus_tag="LK694_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053589.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1668..>1808
                     /locus_tag="LK694_002793"
     CDS             1668..>1808
                     /locus_tag="LK694_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HBK5053590.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFG"
BASE COUNT          650 a          294 c          382 g          482 t
ORIGIN      
        1 ttttaccaaa gggagataaa ggtgatcctg gaataaatgc cacaactaca gctgtagcta
       61 caactacagc aaatgggttg atgtcgtccg cagataaaac taagttggat ggaattgcag
      121 ctggagcaca gaaaaatcca ggtaatgcta caactacgac agcaggttta atgtcagcaa
      181 ctgacaaagc taaactcgat ggattagcca atattacatt tgagaaagtg gggacggttt
      241 aatggctgat attgtgcaat taaaagaaaa gggcgttcct aagtatttga aaactcatgc
      301 caaaggaatt gatggtgtgg agggtgtttt agtaaaagcc acaggtaatg aaacggtctt
      361 aggaacgaaa aattttaaag acggattgca attcaatgga ttgcctgtgc aagcaggtat
      421 gatcgagcga gctatcacac tagctgatcg aagtgataca acaaacgtaa ctgatgtgaa
      481 tggaaaaata atcagaatcg gaaacatcgt atttcttact tttaatttta aatgtggcac
      541 atggcccgaa ggatcagaaa ctcgttggat tttaagaatc ccagacggtt ataaacgcga
      601 tcaaggctat ccagcgcaaa ccgcgctttc actagttaga aatgcaagtc agccagcgga
      661 tgcacgtgca ttcattgatc aaagtagcat catacaagcc aaatcaggtt caggtagttc
      721 atatatttca ggtatgtgga tcactcgaga tccttggccg gcttaacaga ttactaattg
      781 gaggaagagt aaaatgaaaa caatttataa agtattatat ccactaggtt acgaagaaca
      841 cgaagtagag gataatttcc caactggttt accttttgta gaagttccac ctattttgtt
      901 tgagaagaag gaagatgaga cggatgaagc tttcggaaga agacaacagt cgcaattctt
      961 taacttcact gaaaataagt gggaagaagc agttacacaa gattattcaa aaaaattaga
     1021 actactagaa aatttatcta taggtttaca ggtagataat gctgcattga aaaaagcaaa
     1081 tgaagagctt actacaaaag cagaaggact agcacaaatt aattcgaaaa ccatgctgac
     1141 atcccttcaa aacacaaaag acattgctac tatcaaagaa caacttgacg gaggaaaata
     1201 atatgtattc ttatgatgat atcaagatga tgtttgactg gaattgtttt actgctgacc
     1261 aagttcgaca attcgttcca ctatgcatta cagacgaaga agcagaaaaa attattaaca
     1321 atgaagagag cgcatcttaa ttgatgtgct ttttattttg attcaaggag ttgtcacatg
     1381 attaatttag gggaatgggg agcgatagca ggatcaataa ccgctatcgt ttctttgatt
     1441 ttattagtaa taaaaccaat tactgcatct ttctcgaaga ttactgagac tctttcaaaa
     1501 gtaagccaca atttagattt gctgactaaa gatttagaat cgagcaaatc agatcgattg
     1561 atgattcatg aagaactaaa gaaacacgat gaaagattag atacacatac agaaaaattg
     1621 gtggaacaca cgcaacaaat caaaactttg tttagggaga gaagaaaatg aataataaaa
     1681 cgttcgaagt actaaaatgg ttcgcactgg taattattcc cgcactagct actttcgtgg
     1741 ggttagttgg taaagcgctc aattggcagt acacagatat ctgtgttgtc atcattactg
     1801 gttttggc
//
LOCUS       DAFBQH010000160         1756 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.167, whole genome shotgun sequence.
ACCESSION   DAFBQH010000160 DAFBQH010000000
VERSION     DAFBQH010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.167"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            272..628
                     /locus_tag="LK694_002794"
     CDS             272..628
                     /locus_tag="LK694_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBK5053591.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            638..1537
                     /locus_tag="LK694_002795"
     CDS             638..1537
                     /locus_tag="LK694_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBK5053592.1"
                     /translation="MGKLAIISDLHVDIKKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYEPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          588 a          296 c          355 g          517 t
ORIGIN      
        1 tttttccaat agttgttaaa ggttttttat tttttatagt attttttgtt atttaagaaa
       61 ggaataaggt taaacaaata acaggttgct ttttgggtct ttttccgttc aagaaactga
      121 acgaagaaaa gggaaagttg tctcttttgt gataagagaa atcataataa acgaatgact
      181 tactttccaa taaattgaga acagtcagaa ccagacaaat tagttgcatc tgatgaataa
      241 agtgattatt ctatgacatg aggtgataca gatgaaatta gaagtgacaa aagaagcaca
      301 agaagtattg aagaataaat tagaagcaga cgatcaactt ttattagata tcgaaaacgg
      361 tgacggtcca tttgcagaaa gccaagtcag ttgccaattg gacacggcat ttcgattgat
      421 cattgtcaaa aaagatacac aaacagatct aagtctttat tctgtagtgg ctgatacacc
      481 agttggacca atcaaaatca aagacagtgc aatactttac atggatgatc catccacact
      541 tgctttagaa ccaacttata atagtttaca attaaaagga ccaagcggct tgagaaaagg
      601 caacttgcaa gttgttcgaa aagactagag ggataaaatg ggaaaattgg cgatcatcag
      661 cgacctacat gtggacatca agaaattcgg cgaatcagag ctgatgcagc tttggcaagt
      721 tttgcaaaag cacaagatca ctcggcttca tctagccgga gatacggcta ataaagtaga
      781 ccgttgccta gctgttgtcg aatttttcgt caataaagga ttatctacga catttcattt
      841 cggcaatcac gagttggcag atgtgcccaa tgaacagatg atggaccatt ttccagaccc
      901 acatttttta aatgaacggt atattgcttt aaatgagcag accgttcttt tagggatgaa
      961 tggctggtac gactatcagt tttccgaatt gaaagattac gaggcaatcg tacgaatgaa
     1021 gcgtttgtat tggtatgatc ggttcatcca gcgtgggaaa acagatctcg aggtcaatgc
     1081 agaaatgctt gcagctgcaa aaaatttgct agaccagctg gaagcaaaaa aacttgaggt
     1141 gattttatcc actcatttcg ttcctaaacg ggcatttatc atgtatcaat ctgctcctta
     1201 tgagcgctgg aacaaattaa atgcattttt aggatcagaa tcattcggag aattacttga
     1261 ccgttattca aatatcaaac aagtagtttt tggccatacg catcgtcgct ttgaagataa
     1321 atccatcggt cacacgattt acagctgcag accttttggt tattattatg aatggcagct
     1381 gacccgagat tttgtcttga aagaacagct gttagatagc tatgaaccca tgaaattacg
     1441 tgtcctcttg cgtgcccatc atccggcctt tcaagcctat caaacacaac atttgcaaga
     1501 ggaatttgaa caggcgatga caatcgttcc gtattgagac agcattagta atgtgtaaaa
     1561 gaaaatacct tgtaaaacat aaggataagt aagagcagta gatccattta tcgtggatat
     1621 gctgcttttt ttgtggaaaa taatcagcga atgtttattt gatcggatga tttgtaactc
     1681 gcagagtact agatgcaagc gtttattctt tttttgaacg ataatcacaa aaaaaaatat
     1741 aatatacatt ttttat
//
LOCUS       DAFBQH010000161         1701 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.168, whole genome shotgun sequence.
ACCESSION   DAFBQH010000161 DAFBQH010000000
VERSION     DAFBQH010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.168"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..255
                     /locus_tag="LK694_002796"
     CDS             <1..255
                     /locus_tag="LK694_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053593.1"
                     /translation="NFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSVI
                     SEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD"
     gene            258..411
                     /locus_tag="LK694_002797"
                     /pseudo
     CDS             258..411
                     /locus_tag="LK694_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            434..1639
                     /locus_tag="LK694_002798"
     CDS             434..1639
                     /locus_tag="LK694_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBK5053594.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT          547 a          322 c          377 g          455 t
ORIGIN      
        1 aatttcagag tggctttagg tcgtatgtct gaaatgggct atggcatgat gtttggcagt
       61 gacgtacaaa aggatttctt cttaaagcga atcaaaggtc gtggctatgt tgatgtagga
      121 acaagtgtca tatcagagtt ttatactccc cttgtaccaa aaggatatga tttcttggag
      181 gaaattaaaa agttatccaa cagcagacag tccacgcagg cgacgtgcga agcggaagtc
      241 gcaggtgtgg actgatcttg ctggctggtg tggcaatagc cacgccagca cttaacccct
      301 cggtatctaa caggggggta caaatcgaca ggaaacagtc aaaaaaacat tagaaaatcc
      361 tttggttaca agggatttac aaaatttcag cgtatgtcaa atgggcttta aaagttgaca
      421 tacgcctttt tgattggagg gatttttact gaatgaacaa acttggttac agcatttaaa
      481 agaaaaacgc ttggcttatg gactatctca aaaccgttta gctgttgcga ctggtattac
      541 aaggcagtat ctaagcgata ttgaaacagg aaaagtcaag ccatcagagg atttacagca
      601 gtccctttgg gaagctctgg aacgcttcaa tcccgacgct ccccttgaaa tgctgtttga
      661 ttatgtaaga attcgctttc cgacaacaga cgtacagcag gtggtcgaaa acatcttaca
      721 actgaaactg tcctattttc ttcatgagga ctatggtttc tattcttatt cagagcatta
      781 tgctttaggc gacatattcg tcctttgctc ccatgaactg gacaaaggag ttctggtgga
      841 attgaaaggt cgtgggtgca gacaatttga aagctatctt ctggcacaac aaagaagctg
      901 gtatgagttc tttatggacg ttttggtggc tggcggtgtg atgaaacgcc ttgaccttgc
      961 cattaacgat aagacaggga ttttaaatat ccctgtactc actgaaaagt gccaacagga
     1021 agaatgtatc tccgtcttcc gcagttttaa aagctatcgc agtggcgaac tggtacgcaa
     1081 agaggaaaag gaatgtatgg gaaacaccct ctatatcggt tcattacaaa gtgaagttta
     1141 tttctgtatc tatgaaaagg actacgagca gtacaagaaa aatgatattc ccattgaaga
     1201 cgcagaagta aaaaaccgtt ttgagattcg attgaaaaat gagcgtgcct attatgcagt
     1261 ccgtgattta ctcgtctatg acaatccaga gcataccgcc tttaaaatta tcaatcggta
     1321 tatccgtttt gtagataaag acgattccaa acctcgttct gattggaaac tgaatgaaga
     1381 atgggcttgg tttattggga acaatcgtga acgattaaaa ctaaccacaa aaccagagcc
     1441 ttactccttc caaaggacgc tgaactggct atctcatcaa gttgccccga ccttaaaggt
     1501 tgcgattaaa cttgatgaaa tcaaccagac gcaggttgta aaagacattc tcgaccatgc
     1561 gaaactgaca gaccgacaca agcagatttt gaagcaacag tcagtaaaag aacaggacgt
     1621 gataacaaca aaaaaataac tcaaatacaa attcattgaa tatagagagg agaacatttt
     1681 tatgaatttt ggacaaaacc t
//
LOCUS       DAFBQH010000162         1700 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.169, whole genome shotgun sequence.
ACCESSION   DAFBQH010000162 DAFBQH010000000
VERSION     DAFBQH010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.169"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..207
                     /locus_tag="LK694_002799"
     CDS             <1..207
                     /locus_tag="LK694_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBK5053595.1"
                     /translation="AIINWARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELG
                     MICGKLDRQYYSASAISSIVIPPK"
     gene            220..1128
                     /locus_tag="LK694_002800"
     CDS             220..1128
                     /locus_tag="LK694_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBK5053596.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1128..>1700
                     /locus_tag="LK694_002801"
     CDS             1128..>1700
                     /locus_tag="LK694_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053597.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAESYTN"
BASE COUNT          595 a          226 c          381 g          498 t
ORIGIN      
        1 gcaattatta attgggcaag aggtggacaa gtgggttcat ggtttgcaga cggaacttat
       61 ggacatactg gcgttatccg aggcttagct aatggtcgta tgcaaactta tgaacaaaac
      121 acagagttag gtatgatttg tggaaagtta gatcgccagt attatagtgc aagtgcaatt
      181 tcttccattg tcataccacc aaaataggga ggtgatttaa tggcaaagtg gaatgtcata
      241 ttaagtacaa cagaacccta taattacgtg gggatgattc aagttcgaca aggcaataaa
      301 aacactgaag ttatggaagc tacaattgtc gaaaatggtc ttccatatga tttatcagaa
      361 tgtaaggtat attttgaatc agttgttggc ggaaaatatc cagtccaatt agaaacaaga
      421 attgtggatg ctaaaaaagg gaaaatcaaa tatatttttg ataaatattc catgcagtgt
      481 ttgcatcgac aaacagccaa tttcattata tttaaaggag aagacttgat tggaacaact
      541 caagacttct cctattttgt catcaatgct gtttcaaaaa cagaaggaga aatgggttct
      601 tattggcaat caattgaaga tttaattgct gatatgacag attttattaa tgaaaataaa
      661 ggcgatttta cggactggat gaatgaaagg aaagaagaat ttgatcgctg gagagaagaa
      721 caggagaata gtttccaaga ttggagagaa ggacaagaat cagattattt gtcatggttt
      781 gaatcaatca aggatatttt gaagtctatc gatccaggtg gagtaatgtt agccgaatta
      841 atggatgcac gtgttgatat tcaaggagtt cgtcatgcgt ctatttcaga gcgcttgctg
      901 gcagatatgg attatctata tcagaaatta cgagcaacac ttttcacgat tgaatacggt
      961 gagattgaag tgactgatat tttgcaggat gatctctttt cagataatca cgaagtcgaa
     1021 aaagttggaa ctgtagaatt tccaattgaa gaaggagctt tgattattgc aactgttgat
     1081 gatccaaaac agaatgtttt cactcttgag aaagttgggg tgatttgatg gttaaagtaa
     1141 agagaatgat ggagactgat gaatctggtg ttaaacgtca atttatgcca attacacatg
     1201 tttctgcagt tttaggctta gaaaaaatga tatcaggaca atcaaaagtt ctttcggtta
     1261 atggtaaata tggtgcagtc attttaacga aagctgattt aggattagag aatgcaatta
     1321 ctgaactacc ttatgcaagc gagacaagcg atggtattct tactgctgaa atgtttcaaa
     1381 agatcgtaaa tggagaaggt ggaacatata ttttgccgat tgctactcct gaacaattag
     1441 gaggtataaa agttggggaa ctcttagaag ttactgaaga aggtgtttta tctgcaacta
     1501 agcaaacaga cttcaatttc tctgaagaat taaagggaaa attagaatct ctaaaaatat
     1561 taaaagcagg tgcaaatata tcaattgcag aagatggaac aattagttct acaggtggta
     1621 gtgggactgg tggtgttaat cagtcatatg tagatcaaaa attccaagaa gctgtaaatc
     1681 aagcagaaag ttacacaaac
//
LOCUS       DAFBQH010000163         1700 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.170, whole genome shotgun sequence.
ACCESSION   DAFBQH010000163 DAFBQH010000000
VERSION     DAFBQH010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.170"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..207
                     /locus_tag="LK694_002802"
     CDS             <1..207
                     /locus_tag="LK694_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBK5053598.1"
                     /translation="AIINWARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELG
                     MICGKLDRQYYSASAISSIVIPPK"
     gene            220..1128
                     /locus_tag="LK694_002803"
     CDS             220..1128
                     /locus_tag="LK694_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBK5053599.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMSAFINENKGDFTDWMNARKKE
                     FEQWRQDQQNTFEAWREGQETDYLKWFESIKDILKSIDPGGVMLAELMDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1128..>1700
                     /locus_tag="LK694_002804"
     CDS             1128..>1700
                     /locus_tag="LK694_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053600.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTN"
BASE COUNT          597 a          235 c          378 g          490 t
ORIGIN      
        1 gcaattatta attgggcaag aggtggacaa gtgggttcat ggtttgcaga tggaacttat
       61 ggacatactg gcgttatccg aggcttagct aatggtcgta tgcaaactta tgaacaaaac
      121 acagagttag gtatgatttg tggaaagtta gatcgccagt attatagtgc aagtgcaatt
      181 tcttccattg tcataccacc aaaataggga ggtgatttaa tggcaaagtg gaatgtcata
      241 ttaagtacaa cagaacccta taattacgtg gggatgattc aagttcgaca aggcaataaa
      301 aacactgaag ttatggaagc tacaattgtc gaaaatggtc ttccatatga tttatcagaa
      361 tgtaaggtat attttgaatc agttgttggc ggaaaatatc cagtccaatt agaaacaaaa
      421 attgtggatg ctaaaaaagg gaaaatcaaa tatatttttg ataaatattc catgcagtgt
      481 ttgcatcgac aaacagccaa tttcattata tttaaaggag aagacttgat tggaacaact
      541 caagacttct cttattttgt catcaatgct gtttcaaaaa cggaaggaga aatgggttct
      601 tattggcaat caattgaaga tttaattgcg gatatgtcag cttttatcaa tgaaaacaaa
      661 ggtgacttca cagattggat gaatgcacgt aaaaaggaat ttgaacaatg gcgacaagac
      721 caacaaaata catttgaggc atggagagaa ggacaagaaa ccgattattt aaaatggttt
      781 gaatcaatca aagatatttt gaagtctatc gatccaggtg gagtaatgtt agccgaatta
      841 atggatgcac gtgttgatat tcaaggagtt cgtcatgcgt ctatttcaga gcgtttgctg
      901 gcagatatgg actatttata tcagaaattg cgagcaacgc ttttcacgat tgaatacggg
      961 gagattgaag tgactgatat tttgcaggat gatctctttt cagataatca tgaagtcgaa
     1021 aaagttggaa ctgtagcatt cccgattgaa gaaggagcct tgatcatcgc aaccgttgat
     1081 gatccaaagc aaaatgtgtt cactcttgag aaagttgggg tgatttgatg gttaaagtaa
     1141 agagaatgat ggaaactgat gaatctggtg ttaaacgtca atttatgcca attacacatg
     1201 tttctgcagt tttaggatta gaaaaaatga tatcaggaca atcaaaagtt ctttcggtta
     1261 atggtaaata tggtgcagtc attttaacga aagctgattt aggattagag aatgcaatta
     1321 ctgaactacc ttatgcaagc gagacaagcg atggtattct tactgctgaa atgtttcaaa
     1381 agatcgtaaa tggagaaggt ggaacatata ttttgccgat tgctactcct gaacaattag
     1441 gaggtataaa agttggggaa ctcttagagg ttactgaaga aggtgtttta tctgcaacta
     1501 agcaaacaga cttcaatttc tctgaagaat taaagggaaa attagaatct ctaaaaatat
     1561 taaaagcagg tgcaaatata tcaattgcag aagatggaac aattagttct acaggtggta
     1621 gtgggactgg tggtgttaat cagtcatatg tagatcaaaa attccaagaa gctgtaaatc
     1681 aagcagaaaa ttacacaaac
//
LOCUS       DAFBQH010000164         1647 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.171, whole genome shotgun sequence.
ACCESSION   DAFBQH010000164 DAFBQH010000000
VERSION     DAFBQH010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.171"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(20..91)
                     /locus_tag="LK694_002805"
     tRNA            complement(20..91)
                     /locus_tag="LK694_002805"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="LK694_002806"
     tRNA            complement(113..200)
                     /locus_tag="LK694_002806"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="LK694_002807"
     tRNA            complement(225..298)
                     /locus_tag="LK694_002807"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="LK694_002808"
     tRNA            complement(312..382)
                     /locus_tag="LK694_002808"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="LK694_002809"
     tRNA            complement(384..459)
                     /locus_tag="LK694_002809"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(478..551)
                     /locus_tag="LK694_002810"
     tRNA            complement(478..551)
                     /locus_tag="LK694_002810"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Met,seq:cat)
     gene            complement(564..653)
                     /locus_tag="LK694_002811"
     tRNA            complement(564..653)
                     /locus_tag="LK694_002811"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Ser,seq:tga)
     gene            complement(677..750)
                     /locus_tag="LK694_002812"
     tRNA            complement(677..750)
                     /locus_tag="LK694_002812"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Ile,seq:cat)
     gene            complement(764..837)
                     /locus_tag="LK694_002813"
     tRNA            complement(764..837)
                     /locus_tag="LK694_002813"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Met,seq:cat)
     gene            complement(865..937)
                     /locus_tag="LK694_002814"
     tRNA            complement(865..937)
                     /locus_tag="LK694_002814"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Ala,seq:tgc)
     gene            complement(957..1030)
                     /locus_tag="LK694_002815"
     tRNA            complement(957..1030)
                     /locus_tag="LK694_002815"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(994..996),aa:Pro,seq:tgg)
     gene            complement(1040..1113)
                     /locus_tag="LK694_002816"
     tRNA            complement(1040..1113)
                     /locus_tag="LK694_002816"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1077..1079),aa:Arg,seq:acg)
     gene            complement(1124..1209)
                     /locus_tag="LK694_002817"
     tRNA            complement(1124..1209)
                     /locus_tag="LK694_002817"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1173..1175),aa:Leu,seq:taa)
     gene            complement(1226..1297)
                     /locus_tag="LK694_002818"
     tRNA            complement(1226..1297)
                     /locus_tag="LK694_002818"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1263..1265),aa:Gly,seq:gcc)
     gene            complement(1309..1381)
                     /locus_tag="LK694_002819"
     tRNA            complement(1309..1381)
                     /locus_tag="LK694_002819"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1346..1348),aa:Thr,seq:tgt)
     gene            complement(1399..1480)
                     /locus_tag="LK694_002820"
     tRNA            complement(1399..1480)
                     /locus_tag="LK694_002820"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1444..1446),aa:Leu,seq:tag)
     gene            complement(1488..1560)
                     /locus_tag="LK694_002821"
     tRNA            complement(1488..1560)
                     /locus_tag="LK694_002821"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1525..1527),aa:Lys,seq:ttt)
     gene            complement(1564..1636)
                     /locus_tag="LK694_002822"
     tRNA            complement(1564..1636)
                     /locus_tag="LK694_002822"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1601..1603),aa:Val,seq:tac)
BASE COUNT          438 a          460 c          391 g          358 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
      481 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc
      541 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
      601 ccgcctgacg gttttcaaga ccgttccctt cagccggact tgggtaatcc tccatatgaa
      661 acttacaacg ccacaatgga ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
      721 gtctgctcta accaactgag ctaaaggtcc aggctcttaa aaatcgcggc ggaggggatc
      781 gaacccccga cctcccgggt atgaaccgga cgctctagcc agctgagcta caccgcgaaa
      841 aagatattca ttatttaaat gaaatggagc ctagcgggat cgaaccgctg acctcctgcg
      901 tgcaaagcag gcgctctccc agctgagcta aggccccgtg tttaaattta taattatcgg
      961 gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta ccaagctgag
     1021 ctacttcccg tttaattaac gcgcccaaga ggagtcgaac ccctaacctt ttgatccgta
     1081 gtcaaacact ctatccagtt gagctatggg cgcatatttg aaatgccgag gaccggaatc
     1141 gaaccggtac ggtgatcact caccgcagga ttttaagtcc tgtgcgtctg ccagttccgc
     1201 caccccggcg cgattgcttt ggcaaagcgg aaaacggggt tcgaacccgc gacccccacc
     1261 ttggcaaggt ggtgctctac cactgagcta tttccgcgtg tttaaagatg ccggctaaag
     1321 gacttgaacc ctcgaccctc tgattacaaa tcagatgctc taccaactga gctaagccgg
     1381 catgtaaaac ttaaattatg cgggtgaagg gacttgaacc cccacgccgt aaggcgctag
     1441 atcctaaatc tagtgcgtct gccaattccg ccacacccgc aaaaatatga gccgtacagg
     1501 gctcgaacct gtgaccctct gattaaaagt cagatgctct accaactgag ctaacggctc
     1561 atatggaggt taacgggatc gaaccgctga ccctctgctt gtaaggcaga tgctctccca
     1621 gctgagctaa acctccatgg ataactt
//
LOCUS       DAFBQH010000165         1636 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.172, whole genome shotgun sequence.
ACCESSION   DAFBQH010000165 DAFBQH010000000
VERSION     DAFBQH010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.172"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(183..1502)
                     /locus_tag="LK694_002823"
     CDS             complement(183..1502)
                     /locus_tag="LK694_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HBK5053601.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDKRAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          485 a          300 c          325 g          526 t
ORIGIN      
        1 attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac
       61 taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa
      121 ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg
      181 gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga
      241 tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac taatttccca
      301 gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca
      361 aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg
      421 acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc agctttcgct
      481 tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga
      541 gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct
      601 tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg
      661 gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat
      721 ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt
      781 tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag
      841 atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt
      901 acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc
      961 aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc
     1021 gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc
     1081 gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca
     1141 tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt
     1201 tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa
     1261 tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca
     1321 tgagtaaaat acgctcgttt gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa
     1381 ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca
     1441 cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc
     1501 atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt
     1561 cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt
     1621 ttcacatggt tttaat
//
LOCUS       DAFBQH010000166         1627 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.173, whole genome shotgun sequence.
ACCESSION   DAFBQH010000166 DAFBQH010000000
VERSION     DAFBQH010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.173"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..132)
                     /locus_tag="LK694_002824"
     CDS             complement(<1..132)
                     /locus_tag="LK694_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBK5053602.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..1516
                     /gene="bsh"
                     /locus_tag="LK694_002825"
     CDS             542..1516
                     /gene="bsh"
                     /locus_tag="LK694_002825"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBK5053603.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          578 a          230 c          285 g          534 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaataaac
      541 tatgtgtacg tctattactt atgtaacaag tgatcattat tttggaagga attttgatta
      601 tgaaatatct tacaatgaag tagttactgt tactccaaga aattataagt tgaattttcg
      661 aaaggtaaat gatttggata ctcattatgc aatgattggt attgccgctg gtatagctga
      721 ctaccctctt tattacgatg cgacaaatga aaaaggattg agtatggctg ggctaaattt
      781 ttctgggtat gctgattata aagaaataca agaagggaaa gacaatgtat ctccttttga
      841 atttattcct tggattttag gacaatgctc aacagtagga gaaactaaaa aattgttaaa
      901 aaatatcaat ttagcaaata taaattatag tgatgaactt cctttatccc ctttacattg
      961 gctattagct gataaagaaa aatcaattgt cattgaaagt atgaaagatg gacttcatat
     1021 atatgataac cctgtgggcg ttcttaccaa taatccttca tttgactatc aattatttaa
     1081 tttaaacaat tatcgtgtct tatcgagtga aactcctaaa aataattttt caaatcaaat
     1141 aagtttgaat gcctatagcc gcggtatggg agggataggc ttgcctggag atttatcctc
     1201 agtatctcgt tttgttaaag cgacttttac gaagctgaat tctgtatctg gagattcaga
     1261 gtcagaaagt attagtcaat ttttccatat cttaggttca gtagaacaac aaaaaggttt
     1321 gtgtgatgtt ggtgatggaa aatatgaata tacaatttat tcttcttgtt gcaatgttga
     1381 caaaggaatc tattattatc gaacatatga agacagtcaa attactgcaa ttgatatgaa
     1441 taaagaagac ttagatagtc ataagttaat tagttatcca attatagaaa aacaacaaat
     1501 taaatatata aattagttaa tgtgttgtga ttgattattt aatatagata taatacaaaa
     1561 aggcaaagat attttcaaaa cattttttct tgatgtgata tccttgtctt ttttgtaatg
     1621 tttttaa
//
LOCUS       DAFBQH010000167         1618 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.174, whole genome shotgun sequence.
ACCESSION   DAFBQH010000167 DAFBQH010000000
VERSION     DAFBQH010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.174"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(1..305)
                     /locus_tag="LK694_002826"
                     /pseudo
     CDS             complement(1..305)
                     /locus_tag="LK694_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            complement(379..660)
                     /locus_tag="LK694_002827"
                     /pseudo
     CDS             complement(379..660)
                     /locus_tag="LK694_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
     gene            1122..>1618
                     /locus_tag="LK694_002828"
     CDS             1122..>1618
                     /locus_tag="LK694_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053604.1"
                     /translation="MFSHKERVRAIQLFLKYDCSYAATIRELGYPSIGALRKWYKEYL
                     ESGELHQEHRKKSKYSEEQKRIAVNHYFEYGQCYARTIRMLGYPNRESLRQRCEELAP
                     GARKLRKSAVKLTQDQKEAVLKKFYKPQTNRKNLAESGGISRVTLYQWKNTVLGKDFP
                     LCMTL"
BASE COUNT          562 a          284 c          266 g          506 t
ORIGIN      
        1 atatctaagg ttaagccgtc cgcatcaatt gcacgataaa gataatgcca acgtccctta
       61 attttgatat aggtttcgtc cattttccat gaatagaagg attgtctatt tttcttcttc
      121 caaagataat agaggacttt gctgtactct tatacccaac gataaatcgt agtatgacaa
      181 acatttattc cacgatcata taacaattcc tgaacttcac gatagcttag attgtaacgc
      241 aggtagtaac caacagcgac aataatgacg tcttttttga attgtttgcc tttaaaatga
      301 ttcattactc tgtcctctct gtcttttttc tcaattttac actaaaatag attttttgga
      361 aaactttgca acagaaccga aatgagtata gtatgaatta gtaatttggt tttcatctaa
      421 gtttagcttt gatttactaa aattgatatc tcgatacatg ttcaatacat ctaatacaaa
      481 tcgagaatct tctttcggaa atggctcaga aagatcactc caaatttcat catcataatg
      541 atactcgtaa ccttctctca atatttcgat ttttctttca taataagatc tgttttggtc
      601 ctcaaattta gccaataact caaattgatt tactagtgac aatctctgaa aactgtccat
      661 taaatgtttc ttcctatctt gttcattaac aaaaaattat aagcccttac aacgaatatt
      721 atatattaaa tttaattgta ggtgaacacg aattttataa atatttatgg tgttaatgac
      781 aaatgatatc cgactcctct tcatacgttc tggactattt gctaatagat taaaatttca
      841 gcatctattt ctgaccataa ataaatgagc tgccgaaaag ctgccaaatc agacttgaaa
      901 ataaaggatc aaaaactttt aagtttctga ccccgtaact cacacttcaa aatcttactt
      961 aaaaaggagt ctgaaagaaa aaaagagtat tcaaaatttt tttctaacta gctacatcac
     1021 atctatgcat tgacactcaa agaaccagaa taagtcatca gaaagcaagg gggtgcggac
     1081 ataatcctgg acgaaaaaat aaacttagga ggaatgtccg tgtgttttcc cacaaagagc
     1141 gtgtaagggc aattcagtta tttttaaaat acgattgctc ttatgctgca accattcgag
     1201 aattagggta tccgtcgatt ggtgcactac ggaaatggta taaagagtat ttggaatccg
     1261 gagagctcca tcaggagcac cgaaaaaaat caaagtattc tgaagaacaa aaacgaatag
     1321 cggtgaatca ttattttgaa tatggtcaat gttatgcacg aaccattcgc atgcttggtt
     1381 atcctaatag agagtccttg cggcagcggt gtgaagaact tgcaccagga gctagaaaac
     1441 tccgaaaaag tgcggtaaag ttaacacaag atcaaaaaga agctgtgttg aaaaaattct
     1501 ataaaccgca aactaatagg aaaaatttag ccgaatctgg aggaattagt agagttactc
     1561 tatatcaatg gaaaaacacg gttcttggaa aggattttcc attgtgtatg actttaaa
//
LOCUS       DAFBQH010000168         1594 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.175, whole genome shotgun sequence.
ACCESSION   DAFBQH010000168 DAFBQH010000000
VERSION     DAFBQH010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.175"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..1485
                     /locus_tag="LK694_002829"
     CDS             <1..1485
                     /locus_tag="LK694_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBK5053605.1"
                     /translation="CEAKFSENSYGFRPNRSVENAIKATYNRLQISQLHYVIEFDIKG
                     FFDNVNHSKLIKQIWAMGIRDKHLIFILKRILKAPIKMTNGTITYPEKGTPQGGIISP
                     LLANIVLNELDHWVESQWQENPVTKNYVVHINKSGSPCKSNAYKEMKKTKLKEMYMVR
                     YADDFRVFCRYKESAEKAKIAITQWIEQRLKLEVSQEKTRIVNVRKRYSDFLGFKIKM
                     IPRRKKLVVKSHISDKQFKNQKDKLVSQAKKIASPSKGKTELEETRLYNSMVLGMQNY
                     YQIATNVNLDCMKLNRAVMAIFTNRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYV
                     AGEGEPIFPVGYIQHRKPMAKVYLANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENR
                     TIEYADNRISLFSAQHGKCAITLEEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAV
                     HRLIHAKTEETIQKYIALLKLNKVQLIRLNKYRVLAGNQELNLI"
BASE COUNT          632 a          251 c          307 g          404 t
ORIGIN      
        1 tgcgaagcta aatttagtga aaacagctat ggttttagac ctaataggtc agttgaaaat
       61 gcaataaaag caacctataa ccgcctacaa atcagccagc tacattatgt gattgaattt
      121 gatattaagg gtttctttga caacgtgaac cattccaagc tgataaaaca gatatgggct
      181 atgggaatac gggacaagca cttgatattc attttaaaaa ggatattaaa agctccaatc
      241 aaaatgacta acggtacaat aacatatcca gagaaaggaa ctccgcaagg gggaataata
      301 tccccactac tagcaaacat tgttcttaat gagttagacc attgggtaga aagccaatgg
      361 caggaaaatc ctgttacaaa aaactatgtg gttcatatca ataaaagcgg tagcccatgt
      421 aaaagcaacg cttacaaaga aatgaagaaa acaaagttaa aggaaatgta tatggttcgt
      481 tatgcagatg attttagagt tttctgccgc tacaaggaaa gtgctgaaaa agccaaaata
      541 gcaatcactc aatggattga acaacgatta aaattggaag tatcacaaga aaaaacaaga
      601 attgtaaatg ttagaaaaag atactccgac tttcttggtt tcaagattaa aatgataccg
      661 agaagaaaga agttagttgt taaatcacat ataagtgata aacaattcaa aaaccaaaaa
      721 gacaagctgg tatcacaggc aaagaaaatt gcttctccct caaaagggaa aacggaatta
      781 gaagaaacaa gactttataa ttcaatggta ttaggtatgc aaaattatta tcagattgca
      841 acgaatgtaa atctggattg tatgaaactg aatagagctg taatggctat cttcactaac
      901 agattaggta caaacaaaag gggcagattg agaaaaacag gcagacaact tacgacaaag
      961 gaggcaacaa gatatggtaa atctaaaatg cttcggtatg ttgctggcga gggcgaacct
     1021 attttccctg ttggatatat ccaacacaga aaaccaatgg caaaggtgta tttagcgaat
     1081 tgctacactc tagagggaag aaaatttatc cacaccaatc taagtgttga taaattactg
     1141 atgtaccagt taatgaaatt atctttggaa aatcgcacaa tagaatacgc agacaatcga
     1201 atttcgctat tttctgctca acatggaaaa tgtgctataa ctctggaaga atttcaacaa
     1261 gctaatgaaa tacattgtca tcatattgta ccaactggag ctggtggaaa tgacgattat
     1321 aaaaatctta ttttggtaaa agaggcggta caccgtctaa tacacgctaa aacagaagaa
     1381 acaatacaaa aatatatagc tctattgaag ctaaataaag tccagctcat aagactaaat
     1441 aaatatagag tattagctgg caatcaagaa ttaaacttga tataggtcta ctacataaat
     1501 acttttcaat ataaacacga tggaacgcgg agtgcggtga aagtcgcacg ctccgtgtga
     1561 atggggggaa aagtttgaga tatttataat atca
//
LOCUS       DAFBQH010000169         1476 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.176, whole genome shotgun sequence.
ACCESSION   DAFBQH010000169 DAFBQH010000000
VERSION     DAFBQH010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.176"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..772
                     /locus_tag="LK694_002830"
     CDS             <1..772
                     /locus_tag="LK694_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBK5053606.1"
                     /translation="ISNMSKLVAEDVLAFKERTLEANYSVIFMDATHIPVKRQTVSKE
                     AVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEE
                     SIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMT
                     RKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPN
                     EESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
     gene            complement(1112..>1476)
                     /locus_tag="LK694_002831"
     CDS             complement(1112..>1476)
                     /locus_tag="LK694_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HBK5053607.1"
                     /translation="KRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGF
                     YQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGT
                     LFGFSVCTEIKVLLGIPA"
BASE COUNT          469 a          250 c          262 g          495 t
ORIGIN      
        1 aatctccaac atgagtaaat tggtggctga agatgtttta gcttttaaag aaagaacgtt
       61 agaagccaat tattccgtta tatttatgga tgcgacgcat atccctgtga agcgacagac
      121 tgtttcgaag gaagcagtgt atattacgat tggtattcgt ttggatggaa ccaaagaagt
      181 tcttgggttt actattgccc caactgagtc tgcttatatt tggaaagaag ttcttcaaga
      241 tcttagaaaa cgtgggttag aagaagtttt attagtagtg acagacggat taagctgtat
      301 tgaagaaagt atccatagtg tgtatccgaa tgcccaattt caacaatgtt gtgtgcatgt
      361 atctagaaat atcgctcata aagttcgtgt tcgaggtcga aaagaaattt gtgaggattt
      421 caaattggtt taccaagcga attcaaaaga agaggcattg gatcacatcg actttatgac
      481 taggaaatgg aaaaagcagt atccaagagt cgtcaattta ctcttgaatc ctgccctatt
      541 aaccttttat aatttccctc acgccatcag acgaacaatt tattcgacga acctgattga
      601 aggctttaat aagcagctaa aacgatatac tcgaagaaaa gaacaattcc ctaatgaaga
      661 atctctagag agattcttca tttctcaatt taatcaatat aaccaaaaat ttttaggtag
      721 aattcacaag ggatttaaag aaattcagga tacattagag tcgatgattt aactgtaatt
      781 aacagaatgg attttccatt tacatataat tcttgacgct accataatct aaataaccat
      841 catattcatc tactacttat ataattgagt tttattaaac tttttaagtt tctattctat
      901 caaatattta aacaatctct gcatccgaaa agactacctc gtatagtttt actaacaata
      961 tttttattag cgtgttcaaa tcctgatttc ttgtatcaat tattttctgt tcactttcaa
     1021 taagaaaaaa gtctttactt ccatggataa taataagggt tctgttgcaa agttttaaat
     1081 aaagaataaa atcccttacg gtatctatga tttaagctgg gattcccaat aataccttga
     1141 tttcagtaca gaccgaaaac ccgaagagag tgccttcttt tcgggttttc ttatataatc
     1201 ctcggatggc ttccatgcct ttaatcgttg tagaggcagt gcgtaaactt cgatagaatt
     1261 tattgcgtct ctttactgga cgatggtctt gttcaatcaa attattcagg tatttaatgg
     1321 tacgatgttc tgtcccttga taaaagccgt attcttttag tttcttaaag gcacttgtaa
     1381 tagagggggc tttatctgtg actacaacct tcggttcatc aaactgcttc actaaccgct
     1441 taagaaaagc ataggctgct tgtgtgtccc gttttt
//
LOCUS       DAFBQH010000170         1457 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.177, whole genome shotgun sequence.
ACCESSION   DAFBQH010000170 DAFBQH010000000
VERSION     DAFBQH010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.177"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(100..1119)
                     /locus_tag="LK694_002832"
     CDS             complement(100..1119)
                     /locus_tag="LK694_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBK5053608.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1130..1336)
                     /locus_tag="LK694_002833"
     CDS             complement(1130..1336)
                     /locus_tag="LK694_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBK5053609.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          403 a          304 c          246 g          504 t
ORIGIN      
        1 tgggtgatag tgaagatatg cgataacatc actattttat cgaatgattt ttatagttgt
       61 caatatagtt atgtaatgct cctcaacgag gagctatttt tatcgtttag gaatatttaa
      121 ataccaacgt ttgtcatgga aatcttgcgc accgccttta gtgtttccct ctggatcatt
      181 cgttgcccgc atcatgacat agactttctt attagggaag ttacgcatgt taaaagatac
      241 atgatagcca acatttccag aagtattata agcttgattt acatctgatc tataaattcc
      301 atcagctctt actcgagcta attctttccc agtattgtaa tccataatga agatatactc
      361 gtatttatag ttagcaatgt gccatccagc tacatgcaaa tttgcatttt cgatttctcc
      421 aaactgatca atgtgggcgt aattcgttcc atctgtcagt gtaggattag ctgcaccagc
      481 tcgtgttgga tcaatgacag gcttgttttc agaagttgtt ggattttcat cggtaaatcc
      541 atgagctaaa tcatatgcta atttttcttt acttacgccc atttcagaaa gataaccgta
      601 aggatctgta tgatcgcccc agatgttttg tgttacccat aaatgcgatt tgattcccgg
      661 ttggttataa ggagtgtcca acgttaatgg aataccatat ttcattgctg aatctctagc
      721 caattcaacg tatgccttgt agtttttctc aaacgttgct ttatcatgtg tgtgttgtaa
      781 ctcaatctgc acaggactgt tggcattagc atacgaacca gcaccgtact gtacataacc
      841 aggttgaccg acttgataaa caattccgcc gtctcccaca atataagcag tataagcgct
      901 agtccatgaa cgttgcatat actgcgcttc attgcgtcct gttgctgttt cattagccgt
      961 ttcatgcagt aaaatatact gattatttgc tacttgtgag ctaccttcat ttgcgcccaa
     1021 attaaattca ttgttgatag tataggcaaa tccgttaatt ggcaataaaa aaagagccgt
     1081 taacaggctc atcgcagtaa tagtaatttt ctttttcata tctatcctcc tatttatctg
     1141 atgaaatttt cttatacgtt cgatttgata cacctaaaat actccctaga aatgctccaa
     1201 aacctgtaat aatcatcacc gttatatctg tgtaccgcca gttaacacct tttccgacaa
     1261 gaccgacaaa agtcgctaaa gcggggataa ctactatagc gatccatttc aaaatatcaa
     1321 aggttctatt gttcatacta tccacctccc ttcattggtt gggctacttt ctttttcaat
     1381 cgcttgtttt cttctatcaa tatttctctt tctgaaagca aggcatcaat ttgcccgtcc
     1441 agttgttcaa ttttatc
//
LOCUS       DAFBQH010000171         1456 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.178, whole genome shotgun sequence.
ACCESSION   DAFBQH010000171 DAFBQH010000000
VERSION     DAFBQH010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.178"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            108..1040
                     /locus_tag="LK694_002834"
     CDS             108..1040
                     /locus_tag="LK694_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBK5053610.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1456
                     /locus_tag="LK694_002835"
     CDS             1112..>1456
                     /locus_tag="LK694_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053611.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          227 g          420 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagat
//
LOCUS       DAFBQH010000172         1424 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.179, whole genome shotgun sequence.
ACCESSION   DAFBQH010000172 DAFBQH010000000
VERSION     DAFBQH010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.179"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(150..1337)
                     /locus_tag="LK694_002836"
     CDS             complement(150..1337)
                     /locus_tag="LK694_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBK5053612.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DAFBQH010000173         1381 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.180, whole genome shotgun sequence.
ACCESSION   DAFBQH010000173 DAFBQH010000000
VERSION     DAFBQH010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.180"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            35..1336
                     /locus_tag="LK694_002837"
     CDS             35..1336
                     /locus_tag="LK694_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBK5053613.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          448 a          280 c          253 g          400 t
ORIGIN      
        1 gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc atatcaaact
       61 actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga agagcattaa
      121 gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc gctgtccttt
      181 gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa aatcgctcat
      241 tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac aacgctacca
      301 ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata gaaactgctt
      361 tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac aagcaatgac
      421 gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac tcgcttctct
      481 gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa tggatgaatt
      541 tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg gcgatactgg
      601 aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt attttatgca
      661 atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga atgtttctta
      721 tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc gttttcacat
      781 tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga aaagctttga
      841 tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt ctcggcttct
      901 gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca attttggttg
      961 ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg acgaattacg
     1021 aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag aggcggacac
     1081 tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc attctataaa
     1141 aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc cttattcaaa
     1201 tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg cttatggctt
     1261 tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga ttacctatga
     1321 atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat ctcactctat
     1381 c
//
LOCUS       DAFBQH010000174         1368 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.181, whole genome shotgun sequence.
ACCESSION   DAFBQH010000174 DAFBQH010000000
VERSION     DAFBQH010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.181"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            82..>1368
                     /locus_tag="LK694_002838"
     CDS             82..>1368
                     /locus_tag="LK694_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBK5053614.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          467 a          263 c          257 g          381 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaat
//
LOCUS       DAFBQH010000175         1311 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.182, whole genome shotgun sequence.
ACCESSION   DAFBQH010000175 DAFBQH010000000
VERSION     DAFBQH010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.182"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            200..742
                     /locus_tag="LK694_002839"
     CDS             200..742
                     /locus_tag="LK694_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053615.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1248
                     /locus_tag="LK694_002840"
                     /pseudo
     CDS             898..1248
                     /locus_tag="LK694_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414636.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          532 a          148 c          254 g          377 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc t
//
LOCUS       DAFBQH010000176         1296 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.183, whole genome shotgun sequence.
ACCESSION   DAFBQH010000176 DAFBQH010000000
VERSION     DAFBQH010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.183"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..242
                     /locus_tag="LK694_002841"
     CDS             <1..242
                     /locus_tag="LK694_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053616.1"
                     /translation="LPKGDKGDPGINATTTAVATTTANGLMSSTDKTKLDGIAAGAQK
                     NPGNATTTTAGLMSATDKVKLDGLTNITFEKVGTV"
     gene            242..766
                     /locus_tag="LK694_002842"
     CDS             242..766
                     /locus_tag="LK694_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053617.1"
                     /translation="MADIVQLKEDGVAKYLKTHAKGIDGVEGVLVKATGNETVLGTKN
                     FKDGLQFNGLPVQAGMIERAITLADRSDTTNVTDVNGKIIRIGNIVFLTFNFKCGTWP
                     EGSETRWILKIPDGFKRDQGYPAQTALSLVRNASQPADARAFIDQSSIIQAKSGSGSS
                     YISGMWITQDPWPA"
     gene            794..1201
                     /locus_tag="LK694_002843"
     CDS             794..1201
                     /locus_tag="LK694_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053618.1"
                     /translation="MKTIYKVLYPMGYEEHQVEDNFPTSLPFVEVPPILFEKKEDETD
                     EGFGRRQQSQFFNFTENKWEEAVTQDYSKKLELLENLSIGLQVDNAALKKSNEELTEK
                     ADSMAQLNAKLMLNDLTINKKIEAIEKQIGGAS"
     gene            1201..>1296
                     /locus_tag="LK694_002844"
     CDS             1201..>1296
                     /locus_tag="LK694_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBK5053619.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRQFVPLCITDE"
BASE COUNT          472 a          203 c          283 g          338 t
ORIGIN      
        1 ttttaccaaa gggagataaa ggtgatcctg gaataaatgc cacaactaca gctgtagcta
       61 caactacagc aaatgggttg atgtcgtcca cagataaaac taagttggat ggaattgcag
      121 ctggagcaca gaaaaatcca ggtaatgcta caactacgac agcaggttta atgtcagcaa
      181 ccgataaagt gaaactcgat ggattaacca atattacatt tgagaaagtg gggacggttt
      241 aatggctgat attgtgcaat taaaagaaga cggagttgct aagtatttga aaactcatgc
      301 caaaggaatt gatggtgtgg agggtgtttt agtaaaagcc acaggtaatg aaacggtctt
      361 aggaacgaaa aattttaaag acggattgca attcaatgga ttgcctgtgc aagcaggtat
      421 gatcgagcga gctatcacac tagctgatcg aagtgataca acaaacgtaa ctgatgtgaa
      481 tggaaaaata atcagaatcg gaaacatcgt atttcttact tttaatttta aatgtggcac
      541 atggcccgaa ggatcagaaa ctcgttggat tttaaaaatc ccggacggtt tcaaacgtga
      601 tcaaggctat ccagcgcaaa ccgcgctttc actagttaga aatgcaagtc agccagcgga
      661 tgcacgtgca ttcattgatc aaagtagcat catacaagcc aaatcaggtt caggtagttc
      721 atatatttca ggtatgtgga tcactcaaga tccttggccg gcttaacaga ttactaattg
      781 gaggaagagt aaaatgaaaa caatttataa agtattgtat ccaatgggat atgaagaaca
      841 tcaagtagaa gataactttc caacatcttt accatttgtc gaagttccac ctattttgtt
      901 tgaaaagaag gaagatgaaa cggatgaagg tttcggaaga agacaacagt cgcaattctt
      961 taacttcact gaaaataagt gggaagaagc agttacacaa gattattcaa aaaaattaga
     1021 actactagaa aatttatcta taggtttaca ggtagataat gctgcattga aaaaatcaaa
     1081 tgaagagtta acggaaaaag cagattcgat ggcgcagttg aacgccaaac tgatgcttaa
     1141 tgatttaaca attaataaaa aaattgaagc aatagaaaaa caaattggag gtgcttcata
     1201 atgtttagtt ttagcgatgt gaaaatgatg tatgattggg gctgttttac tgacgatcaa
     1261 gttcgacaat tcgttccact atgcattaca gacgaa
//
LOCUS       DAFBQH010000177         1263 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.184, whole genome shotgun sequence.
ACCESSION   DAFBQH010000177 DAFBQH010000000
VERSION     DAFBQH010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.184"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            64..1242
                     /locus_tag="LK694_002845"
     CDS             64..1242
                     /locus_tag="LK694_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="HBK5053620.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVRTLNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDIYSRG
                     VHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRNIAHKVRVKDRKEICDDFKAV
                     YQASSKEEANTFLGSMIEKWQKTYPKVTQSLIKNQDLLTFYEFPPGIRRSIYSTNLIE
                     SFNKQIKKYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
BASE COUNT          424 a          254 c          245 g          340 t
ORIGIN      
        1 aaaggaagtc ccttctgtag aatagagtta ccacaacaca ttcacagaaa agaggacttc
       61 catatgaacg attttactac agaaattcta aagactctag cgaacaaagg cgatttgaat
      121 gaattattcc gtgtccattt ggaaaaagct gtcaatacgc ttctcaaaac ggagttaacg
      181 gctttcctcg attacgaaaa gtacgatcgc attggtttta acacgggtaa ttctcgtaac
      241 ggctcctatg accgtacggt caagaccgag tacggggaac ttcatctcca gattccgcgc
      301 gaccgcaacg gcgagttcaa gcaacagact gttcctgctt atagacggac gaatgatacc
      361 ttagaggaga ccgtcattca cctcttccga aaaggtatta ccatgtcgga aatcgcagac
      421 ttgattgaga aaatgtatgg gcatcactac acgccccaaa ccatgtccaa catgactaaa
      481 gtgctgactg aagaagtaaa tgcatttaaa gttagaactc taaatgataa gtatgtcgct
      541 attttcatgg atgctactta tattccttta aaacggcaaa ccgtctccaa agaagcaatc
      601 tatattgcca ttggtatacg agaagacggc actaaagaag tactaagtta tgcgattgcc
      661 ccgactgaat cgacctacgt ttggaatgag ttgttacagg acatttactc cagaggggtt
      721 catgatgtct tactgtttat taccgatggc ttaaaaggca tgaaagatac gattcaccag
      781 atttatccta aagctaaata ccaacattgc tgtgttcacg tttcccgtaa tattgctcat
      841 aaagtacgtg tcaaagatcg aaaagaaatc tgtgatgatt ttaaggctgt ttatcaagct
      901 agctcaaagg aagaggcaaa taccttttta gggagtatga ttgagaagtg gcagaaaact
      961 tatcctaaag tgacgcagtc actgataaaa aatcaagatt tattgacttt ttatgagttt
     1021 ccgcctggta tccgccgaag catttactca actaatttaa tcgaatcttt caataaacaa
     1081 atcaagaaat acagccgcag aaaagagcag tttcaaaatg aagaatcact agagcgtttc
     1141 ctagtatcca tctttgatac atacaatcaa aaattcttaa atagaagcca taaaggcttc
     1201 caacaagtga cggatacatt agcttcaatg tttactgagt aacccattat tttgcagaag
     1261 gac
//
LOCUS       DAFBQH010000178         1193 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.185, whole genome shotgun sequence.
ACCESSION   DAFBQH010000178 DAFBQH010000000
VERSION     DAFBQH010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.185"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..1076
                     /locus_tag="LK694_002846"
     CDS             <1..1076
                     /locus_tag="LK694_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBK5053621.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAFBQH010000179         1188 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.186, whole genome shotgun sequence.
ACCESSION   DAFBQH010000179 DAFBQH010000000
VERSION     DAFBQH010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.186"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            141..398
                     /locus_tag="LK694_002847"
     CDS             141..398
                     /locus_tag="LK694_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053622.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1061)
                     /locus_tag="LK694_002848"
     CDS             complement(630..1061)
                     /locus_tag="LK694_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HBK5053623.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          453 a          151 c          148 g          436 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatcc
//
LOCUS       DAFBQH010000180         1161 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.187, whole genome shotgun sequence.
ACCESSION   DAFBQH010000180 DAFBQH010000000
VERSION     DAFBQH010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.187"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(275..1156)
                     /gene="galU"
                     /locus_tag="LK694_002849"
     CDS             complement(275..1156)
                     /gene="galU"
                     /locus_tag="LK694_002849"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBK5053624.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          294 a          260 c          181 g          426 t
ORIGIN      
        1 ttaaaaaatt cgccttttta ttctactatg aatcttctgg cttggctttc atttctctta
       61 tgtaatcatt ttcttttata caacttaaaa aaaaattaaa ccattagcat aatattttct
      121 gaatctattc acgaaagtct ttttctatct ttcgttttcc acgactcaac tgatctagag
      181 tcaaaaaact aaaacgacag ttgcgcaaat gacgcacaag ctgccgcttt aataacaaat
      241 agcctttcat tctcagcagg ccacttattt ccagttaact ttctgttctt agctttttac
      301 tcaactggat aatatatgca cttaatgaag tcccaatttc cgaatgttga agtccatatt
      361 caatatttgt cttcatatat cctaacttat cgcctacgtc gtatcttttt cctttaaact
      421 ctctcgcata gaccgcctct gtctggttaa gagtctgaag tgcatccgtt agctgtatct
      481 cgtttcctgc tcctggttct tgagtttcta atatctcaaa tattttagga gtcagtagat
      541 aacgtccgat aatggctaaa ttacttggtg cttttcccat ttcaggtttt tctacaaacc
      601 cttttactct gcaacgaccc ttttcgctat ctgttttagg atcaataatt ccgtatttag
      661 atgtttcatt ttctggtaca cgcatgacgg caacagcgga agcttgtgtt tcttcataat
      721 ccatcatcaa ttgttgagtc aatgggatat catctttcat caaatcatcc cccagcatga
      781 cgacaaacgg ttcatttcct ataaatgctt ttgcttgaag cacggcatcc cccagtcctc
      841 ttggatgaga ttgacggatg aagtgaagat tgacttgagt cgtttcttca actaaagcga
      901 gtaattctgt tttttccttt tcccgcaaat tgttttccaa ttcgatattt gcatcaaagt
      961 gatcttcaat gggtcttttt tcttttcccg ttacgatcaa gatgtcttcg attcccgaat
     1021 ccaatgcttc ttccacaata aactggatcg ttggtttatc cacgattgga agcatttctt
     1081 ttgccatggc ttttgttgcc ggcaaaaaac gtgttcctag cccagcggca ggaatgactg
     1141 cttttttgac cttcacttgc t
//
LOCUS       DAFBQH010000181         1153 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.188, whole genome shotgun sequence.
ACCESSION   DAFBQH010000181 DAFBQH010000000
VERSION     DAFBQH010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.188"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            10..210
                     /locus_tag="LK694_002850"
     CDS             10..210
                     /locus_tag="LK694_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBK5053625.1"
                     /translation="MALGRMSELGYSMMFGEVEKNFFMKHIKGRGYVDTGGSVISEFY
                     TPLVPKGYDFLDSISKLEKMND"
     gene            288..962
                     /locus_tag="LK694_002851"
     CDS             288..962
                     /locus_tag="LK694_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBK5053626.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            955..1119
                     /locus_tag="LK694_002852"
     CDS             955..1119
                     /locus_tag="LK694_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053627.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
BASE COUNT          408 a          155 c          245 g          345 t
ORIGIN      
        1 aactttcgcg tggctttggg tcgtatgagt gaactcggat attccatgat gtttggggag
       61 gtcgaaaaaa acttctttat gaagcacatc aaaggtcgag gttatgtgga tacaggtggc
      121 agtgtgatta gtgagtttta tacgccactt gtaccaaaag ggtatgattt cttagattca
      181 attagtaaat tagaaaagat gaatgattga gtgatcttca atactgactt tatacttcaa
      241 gtaacgtata atacttttat atagtaggaa gaaaggggat aattaatatg gaaagtaatg
      301 attcttattt agagtatgca aaagcaaata aggataagtt tatttcttat attatcaaag
      361 ggaaatcccc cgaagttgga cgagacgcag tttttatggc agggagtcct ggggctggaa
      421 aatcagaggt tgccattgga ttagctgaga attatgataa ccatgtgatt attgatgcgg
      481 attactttcg cacccagttt cctgattata atggaaaaaa ctctagtctc tttcaaaaag
      541 catcttcttg gttggtagag caggcattta gatatgtctt agatcatggc tattctttta
      601 ttctggatgc aacttttgca atattaagtg ctgaaaaaaa tatctcgcgg gcgttgaaaa
      661 aacattttcg agtgacgatt ttctatgttt atcaagaccc taaaatcgct tggaaattta
      721 cacaagaacg tgaaattgca gagggacgtc atgtgccgaa aaaggcattt attaacgctt
      781 ttttcaaatc aagagagaac attgcaaaag taaagagtaa acatccagat gtattgcttc
      841 atatcataat aaaagattat acgaataata tttccgaagt tcattatgct acggataatc
      901 tagaattatt tttaccacta gggtatagta ggaaagattt ggaggttcaa ttacatgact
      961 gagaaagaac aagtaacgaa aattgtaaag aaatacaata agagcattgc ggatttatcg
     1021 gaaaatgcta ctgcaaaaga gttcaaaacg gtgattaaat atgtagccga tcaggccaat
     1081 gaaaaacaac ggaaattagt tggattaaat aaaaaatagt aggacaaatt aataatgtat
     1141 gagccaagag caa
//
LOCUS       DAFBQH010000182         1082 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.189, whole genome shotgun sequence.
ACCESSION   DAFBQH010000182 DAFBQH010000000
VERSION     DAFBQH010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.189"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            700..>1082
                     /locus_tag="LK694_002853"
     CDS             700..>1082
                     /locus_tag="LK694_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBK5053628.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMW"
BASE COUNT          413 a          161 c          244 g          264 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 ga
//
LOCUS       DAFBQH010000183         1025 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.190, whole genome shotgun sequence.
ACCESSION   DAFBQH010000183 DAFBQH010000000
VERSION     DAFBQH010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.190"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..>1025
                     /locus_tag="LK694_002854"
     CDS             <1..>1025
                     /locus_tag="LK694_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBK5053629.1"
                     /translation="TTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADTSVAKKHC
                     FISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTPLPKVLCF
                     DEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREAVEWIVID
                     MYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQKHYQLGKQ
                     LKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQLYQDFLY
                     AVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPLECLNNHI
                     KVLKRNA"
BASE COUNT          322 a          215 c          196 g          292 t
ORIGIN      
        1 caacaaccgt ccggttgtta atgaacgatg tttctgaata ccgtacctac ctcgaattga
       61 aaaagcaacg cttcaaatgc aaagcttgtc agcggacctt tgtcgcagat acttctgttg
      121 cgaaaaaaca ttgtttcatc agtgagaaag tccgctggtc tgtcgtgact cgtctgaaaa
      181 aaaatacctc aatgacagag atcgccgctc agaaaaatct atcggtctct tctgtttacc
      241 gtattatgaa gcgattttat cgtccgttga atccttttcg gacaccgtta cctaaggtac
      301 tttgttttga tgaattcaaa tcagttcggg gtgtttcagg tgctatgagt ttcatcatga
      361 tggatggaca aacacagcgc ttattggaca ttgtcgagaa ccgacaactg ccttttttaa
      421 aacgttattt tagtcatttt tcacgagaaa ttcgtgaagc ggtcgaatgg atcgtgattg
      481 atatgtacgc tccttatgta tcgctcgtta aaaagctgtt tccaaaagca cagctgatta
      541 ttgaccggtt tcacatcgtc caacatattg gtcgtacctt tcgcaatcat cgaatcaaag
      601 aaacgaacca actattaaaa agcaaagaac aaaaacacta tcaattaggt aagcaattaa
      661 aaagatactg gaaacttctt caaaaagatg aacgcaagct tgattatact cgacgccttt
      721 ggcgacctgg cttcaaagct catttaacag agaccgatat tgtggatcgc ttactgaaag
      781 ggagccctgc tttgcgtgta ggctatcagt tatatcaaga ctttctttac gctgtaaagg
      841 aacgagacta cgtatcgttt gaagaactac ttaccaataa tatcatgctt cccgaaggct
      901 atcagacgac cttgagaacg ttccagaaat ttctgccaca aatcaaaaat gctttacaac
      961 aaagttattc aaatggccca cttgaatgtc tgaacaacca tatcaaagtg ttaaaacgaa
     1021 atgca
//
LOCUS       DAFBQH010000184          995 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.191, whole genome shotgun sequence.
ACCESSION   DAFBQH010000184 DAFBQH010000000
VERSION     DAFBQH010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.191"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            1..891
                     /locus_tag="LK694_002855"
                     /pseudo
     CDS             1..891
                     /locus_tag="LK694_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          378 a          167 c          166 g          284 t
ORIGIN      
        1 ttaaaaaatc aggaaaaaca aaaagagcta cttttaaagg aagtagaaga cttacaaaaa
       61 caagtacacc aattacagct cgaaaaagca ttactcgaag gagcggctga actgctaaaa
      121 aaagaaaaag gcgtcaatct tctttgtcta tcaaatcaag aaaaaacgat attgattgat
      181 gccttacgaa atcagttcac cttgaaagag cttcttcaac agctccagtt tcctaaaagc
      241 agctattttt atcaaaaaca agcacttgag aaaccagata agtattacaa agaacgtcaa
      301 attaattatc acgattttca atcataactt ctgtgcgtat ggttaccgta gaattcacca
      361 agctttaaaa aatatgggta aaaagctttc tgaaaaagtt gtacgtagat tgatgactga
      421 ggaaaatcta tttgttaaat tttctagacg aaaaaagtat tcttcgtatg ctggggaaat
      481 ttctcctgca caccctaatt tattaaatcg gaattttaaa gcaaagaagc caaatgaaaa
      541 atggttaaca gatattactg aatttagaat tctcgcaggg aaaatctact tatcaccact
      601 tgtcgattgt tacgatggtg ctattgttag ttggactatt ggaacaaagc caacttgctt
      661 cactctatgt ctaaaaaggg ttattcacca gataattcag cgtgcgaagg tttctttgga
      721 cgattaaaaa acgagatgtt ttatcaacgt gactggaaga atacaacaat caatcagttt
      781 ataaacaaat tggatgatta tattcattgg tataataatc aacgaattaa gttatcttta
      841 ggagggctaa gccctcttca atatcggaag aagcaaagat atatttgtta aaaaggtcca
      901 aaaaaaatat ccgcaccccc ttttttttgt aaaaaactat tgcacaatac aagtagccta
      961 cggctatcga aatttccata actttcgagc aagaa
//
LOCUS       DAFBQH010000185          994 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.192, whole genome shotgun sequence.
ACCESSION   DAFBQH010000185 DAFBQH010000000
VERSION     DAFBQH010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.192"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..>994)
                     /locus_tag="LK694_002856"
     rRNA            complement(<1..>994)
                     /locus_tag="LK694_002856"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DAFBQH010000186          890 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.193, whole genome shotgun sequence.
ACCESSION   DAFBQH010000186 DAFBQH010000000
VERSION     DAFBQH010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.193"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..888
                     /locus_tag="LK694_002857"
     CDS             <1..888
                     /locus_tag="LK694_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBK5053630.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DAFBQH010000187          867 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.194, whole genome shotgun sequence.
ACCESSION   DAFBQH010000187 DAFBQH010000000
VERSION     DAFBQH010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.194"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            170..>867
                     /locus_tag="LK694_002858"
     CDS             170..>867
                     /locus_tag="LK694_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053631.1"
                     /translation="MYDILCNNGFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGL
                     VQWDGSSYPLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVV
                     QPTTVDGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEA
                     GLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIA
                     YDWSAVGWKVIQNP"
BASE COUNT          281 a          128 c          216 g          242 t
ORIGIN      
        1 aggaaatggt aaagcaattt attttggaga cttagatgta caaggaaaac ttacagttaa
       61 aggaaaagaa ataattcctg gacaaggtgg tggtccatcg ggaggtggag aatctactgg
      121 tgggtatcct agtgaattaa aaacagatgc agaaaagaga gcttggagaa tttacgatat
      181 tttgtgtaat aacggattta cgaaacaatc tgcatgtgga atattgggga acattcaaca
      241 agaaacagga ggaacttttg atcctgatac tgttcaaata ggtggaccag catacggatt
      301 agttcagtgg gatggttctt catatcctct tgttggtcca gcaacatggg atggaaaagt
      361 ttatgttcaa aacttattta atgctgcagg tattaaagaa ccaataacga gcttagatgc
      421 acaagttcga ttgcttattt ggacatttac aaatggacaa tggatgggcg tagtacaacc
      481 tacgacggtt gatggattta aggcctgtac tgatccaaga caagcagcat atgcttttga
      541 acgaaactat gaacgtccgg cagcgacaca tcctgaacgt caagattatg cagttaactg
      601 gtataacaaa tttaaagatt taaaaccagg aggagctact ggagaagcag gactaaaaca
      661 tttagagtct ttgattggac aacgaattgg caatggtcag tgttatggct tgtctgcaga
      721 atattcagga tatctgggtg gttgtggcat gggtgctgga acaaaatatg gtttaactca
      781 tgtgatagga aatacttctg cagcatctga tattggtatt gcctatgatt ggtctgctgt
      841 tggttggaaa gtgattcaaa accctag
//
LOCUS       DAFBQH010000188          844 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.196, whole genome shotgun sequence.
ACCESSION   DAFBQH010000188 DAFBQH010000000
VERSION     DAFBQH010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.196"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..735
                     /locus_tag="LK694_002859"
     CDS             <1..735
                     /locus_tag="LK694_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK5053632.1"
                     /translation="KQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFI
                     GTMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEG
                     FSHPEMGHMLVVPYPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWE
                     IEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKY
                     IVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          236 a          170 c          210 g          228 t
ORIGIN      
        1 aaacaatacc aagcagattt agcagggatt gggattggct catttgggcc tatcgatatc
       61 catcgcgatt ccgccactta tgggtacatt acctcgaccc cgaaattggc atggcaaaat
      121 tttgatttca tcggtaccat gaaaaaagaa tttccgatcc cgatttcttg gacaacagat
      181 gtcaatgcgg ctgcctatgg tgagtatgtc ttcggcagtg gcaaaggatt gagcagtgtc
      241 gtttactata cgatcggtac gggtgttggc ggcggtgcct tacaagaagg gcgctttatc
      301 gaagggttta gtcatccaga aatggggcat atgctcgtcg tcccttatcc aaaagatgac
      361 tttgctggca gctgtccatt tcatggcaac tgtttagaag ggatggccgc tggacccgcc
      421 atcgaaaaac gcttagggaa aaaaggacaa gaggttgcag aagatgatcc ttattgggaa
      481 atcgaagcct cctatattgc tcaatgtgcc tacaatacaa ccttgatgtt ttctccagat
      541 gtgatcattt ttggcggtgg tgtgatgaaa caacgccata tgctgcaaaa cgtccacgac
      601 gcatttgcaa aattggtcaa tgggtatgtc gaaacacctg agttaagcaa atatatcgtg
      661 accccagcat tagaagacaa cgctggaacg ctcggttgtt tggcattggc tcgagaagcg
      721 gtcttacatt cataaacgaa aaaaggctct tcgacattta gtgttggtag agaaagatac
      781 aactgaaaat ttagtcataa ccagttaggc agagtgctta actggttttt gttttacttg
      841 gaag
//
LOCUS       DAFBQH010000189          816 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.197, whole genome shotgun sequence.
ACCESSION   DAFBQH010000189 DAFBQH010000000
VERSION     DAFBQH010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.197"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..739
                     /gene="aph(2'')-I"
                     /locus_tag="LK694_002860"
     CDS             <1..739
                     /gene="aph(2'')-I"
                     /locus_tag="LK694_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HBK5053633.1"
                     /translation="SILHRSLDLTNFLNTNLETNVKIPNIEYSYISDELSILGYKEIK
                     GTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLR
                     ETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDF
                     GDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIET
                     IVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          351 a           57 c          134 g          274 t
ORIGIN      
        1 tagcatttta cacaggagtc tggacttgac taatttttta aatacaaatt tagaaactaa
       61 tgtaaaaatt cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta
      121 taaagaaatt aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga
      181 acaaaatttg ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta
      241 tacagatatt agtgaatgta ctattgataa taaacaaaat gtattagaag agtatatatt
      301 gttgcgtgaa actatttata atgatttaac tgatatagaa aaagattata tagaaagttt
      361 tatggaaaga ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat gccataatga
      421 ttttagttgt aatcatctat tgttagatgg caataataga ttaactggaa taattgattt
      481 tggagattct ggaattatag atgaatattg tgattttata tacttacttg aagatagtga
      541 agaagaaata ggaacaaatt ttggagaaga tatattaaga atgtatggaa atatagatat
      601 tgagaaagca aaagaatatc aagatatagt tgaagaatat tatcctattg aaactattgt
      661 ttatggaatt aaaaatatta aacaggaatt tatcgaaaat ggtagaaaag aaatttataa
      721 aaggacttat aaagattgat tatataatat atgaaaagct attataaaag acattagtat
      781 taaatagttt aaaaaaatga aaaataataa aggaag
//
LOCUS       DAFBQH010000190          791 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.198, whole genome shotgun sequence.
ACCESSION   DAFBQH010000190 DAFBQH010000000
VERSION     DAFBQH010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.198"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..782
                     /locus_tag="LK694_002861"
     CDS             <1..782
                     /locus_tag="LK694_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK5053634.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          242 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc t
//
LOCUS       DAFBQH010000191          787 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.199, whole genome shotgun sequence.
ACCESSION   DAFBQH010000191 DAFBQH010000000
VERSION     DAFBQH010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.199"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..>787
                     /locus_tag="LK694_002862"
     rRNA            <1..>787
                     /locus_tag="LK694_002862"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          241 a          156 c          231 g          159 t
ORIGIN      
        1 aatatggtag ttctttcaga tagtcgaatg acttggaaaa gtcagtcaaa gagggtaaaa
       61 accccgtaga cgaaatgtgg aagacaccta ggaggatcct gagtacggcg gaacacgaga
      121 aattccgtcg gaatccggga ggaccatctc ccaaggctaa atactcccta gtgaccgata
      181 gtgaaccagt accgtgaggg aaaggtgaaa agcaccccgg aaggggagtg aaatagaacc
      241 tgaaaccgtg tgcctacaac aagtcaaagc ccgttaatgg gtgatggcgt gccttttgta
      301 gaatgaaccg gcgagttacg attgcatgcg aggttaagtt gaagagacgg agccgcagcg
      361 aaagcgagtc tgaatagggc gtttgagtat gtagtcgtag acccgaaacc atgtgatcta
      421 cccatgtcca ggttgaaggt gcggtaaaac gcactggagg accgaaccca cgtacgttga
      481 aaagtgcggg gatgaggtgt gggtagcgga gaaattccaa acgaacttgg agatagctgg
      541 ttctctccga aatagcttta gggctagcct cggaattgag aatgatggag gtagagcact
      601 gtttggacta ggggcccatc tcgggttacc gaattcagat aaactccgaa tgccattcat
      661 tcatatccgg gagtcagact gtgagtgata agatccatag tcgaaaggga aacagcccag
      721 accaccagct aaggtcccaa aatatatgtt aagtggaaaa ggatgtgggg ttgcacagac
      781 aactagg
//
LOCUS       DAFBQH010000192          787 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.200, whole genome shotgun sequence.
ACCESSION   DAFBQH010000192 DAFBQH010000000
VERSION     DAFBQH010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.200"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            24..716
                     /locus_tag="LK694_002863"
     CDS             24..716
                     /locus_tag="LK694_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBK5053635.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          195 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttat
//
LOCUS       DAFBQH010000193          754 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.201, whole genome shotgun sequence.
ACCESSION   DAFBQH010000193 DAFBQH010000000
VERSION     DAFBQH010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.201"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..>754
                     /locus_tag="LK694_002864"
     CDS             <1..>754
                     /locus_tag="LK694_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBK5053636.1"
                     /translation="MTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAKEAIH
                     IVVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAGAVQ
                     RFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFAEKW
                     KTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKKYSHRKEQFQNEE
                     SMERFLVSSFDTYNQKFLGRSHKGFQQAEGELE"
BASE COUNT          224 a          164 c          168 g          198 t
ORIGIN      
        1 atgactaaat catttacgga agaagtcact tcatttaagg aacgtgagct tcatgaccgt
       61 tatgccgcta tttatatgga cgcaacctat atccctttaa agcggaaaac cgtcgccaag
      121 gaagccattc atatcgtagt tggcattcgc ccggacggtt caaaggaagt gttgagctat
      181 gcgattgcac caactgaatc tatcacgatt tgggaagaga ttttaataga cctccaggaa
      241 cgtggcgtga aaaacgtcct ccttttcatc acggatggct taaaggggat ggcaggggcc
      301 gtgcagcggt tctaccccaa agcccgtttt caacattgct gtgtgcacgt ttcccgtaat
      361 atcagtcaca aagtgcgtgt cgatgatcgt aaggaagtct gtgatgattt taaaatggtg
      421 tatcaagcgt catctaaaaa ggcggcattg gaagcacgtg gtgcttttgc ggaaaaatgg
      481 aaaaccagtt atccaaaaat ggtggaatcg atcctttcga acgattacct gctcactttc
      541 tatgatttcc cattagccat acgcaagact atttactcta cgaacttgat tgaatccttt
      601 aataagcaaa tcaagaaata cagccaccgc aaggaacagt tccaaaatga agagtcgatg
      661 gaacgtttct tagtctcgtc ttttgatact tacaaccaaa aattcctagg tcgcagtcat
      721 aaaggtttcc aacaggccga aggcgagctc gaac
//
LOCUS       DAFBQH010000194          736 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.202, whole genome shotgun sequence.
ACCESSION   DAFBQH010000194 DAFBQH010000000
VERSION     DAFBQH010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.202"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            312..608
                     /locus_tag="LK694_002865"
     CDS             312..608
                     /locus_tag="LK694_002865"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK5053637.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLSTVCMKKLDVC"
     gene            complement(531..736)
                     /locus_tag="LK694_002866"
                     /pseudo
     CDS             complement(531..736)
                     /locus_tag="LK694_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          248 a          141 c          106 g          241 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttccacggt ctgtatgaaa aagcttgacg
      601 tctgttaaag aatacggtat ccgtccaaag gcttctttta ccaatgaggc atctttcttc
      661 tcaccacagg aataaccaat aatttctcga ttaaacaaat caagtattaa gcagatataa
      721 tgccactttt tcccca
//
LOCUS       DAFBQH010000195          587 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.204, whole genome shotgun sequence.
ACCESSION   DAFBQH010000195 DAFBQH010000000
VERSION     DAFBQH010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.204"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            26..412
                     /locus_tag="LK694_002867"
     CDS             26..412
                     /locus_tag="LK694_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK5053638.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT          221 a           93 c          118 g          155 t
ORIGIN      
        1 ataaaaataa aaaaggagat ctttcatgaa aaaaatttta gggttgatcg ttatcatatt
       61 gattgctttt ataggatgga aaggttggga ctactatcag tctacttatg taggtaaaga
      121 ttattatgcg gtagtcaaag ctccaatgcc tgcagaaact gatatcaaag cagataatgg
      181 ggaagtaatt ggaagaggtt ttaaatacaa cgtagatgct tatgctgaaa acggtgaaaa
      241 aagacagtta gatttcgatg tcattacttc tggtgactct gccaatggtt ctgcttaccc
      301 cgaaggaacg atcttgcagt tgaaagcaag cgaaaagcgg atcatagaga aaaaagtaat
      361 taccagcgat aaggtaccct catctgtaaa agaaaaatta ggcctatctt aagctaagaa
      421 tatatcgcaa aaaagcactc gaagtgagtg cttttttgct tactgattct agaaaacaaa
      481 aaaacagaat taaccttcga agtcagcctc actgttctta aagtcctaaa acaaactaaa
      541 gagcaaggca atcaatcgat ttctatacaa acaagggact gtgacaa
//
LOCUS       DAFBQH010000196          566 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.205, whole genome shotgun sequence.
ACCESSION   DAFBQH010000196 DAFBQH010000000
VERSION     DAFBQH010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.205"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            30..103
                     /locus_tag="LK694_002868"
     tRNA            30..103
                     /locus_tag="LK694_002868"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:64..66,aa:Met,seq:cat)
     gene            122..194
                     /locus_tag="LK694_002869"
     tRNA            122..194
                     /locus_tag="LK694_002869"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:155..157,aa:Asp,seq:gtc)
     gene            198..270
                     /locus_tag="LK694_002870"
     tRNA            198..270
                     /locus_tag="LK694_002870"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:231..233,aa:Phe,seq:gaa)
     gene            275..345
                     /locus_tag="LK694_002871"
     tRNA            275..345
                     /locus_tag="LK694_002871"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:307..309,aa:Gly,seq:tcc)
     gene            358..431
                     /locus_tag="LK694_002872"
     tRNA            358..431
                     /locus_tag="LK694_002872"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:392..394,aa:Ile,seq:gat)
     gene            443..514
                     /locus_tag="LK694_002873"
     tRNA            443..514
                     /locus_tag="LK694_002873"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:476..478,aa:Glu,seq:ttc)
BASE COUNT          117 a          130 c          147 g          172 t
ORIGIN      
        1 gaatccctta tcctcctttt tcatattatc gcgggatgga gcagtctggt agctcgtcgg
       61 gctcataacc cgaaggtcgt aggttcaaat cctgctcccg caattaaaaa ctaaaaacat
      121 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
      181 ttccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      241 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      301 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      361 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      421 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      481 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      541 tctttttatc tgaaagaatg atttta
//
LOCUS       DAFBQH010000197          563 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.206, whole genome shotgun sequence.
ACCESSION   DAFBQH010000197 DAFBQH010000000
VERSION     DAFBQH010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.206"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..129
                     /locus_tag="LK694_002874"
     CDS             <1..129
                     /locus_tag="LK694_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK5053639.1"
                     /translation="SGITMEKTKKGLLKQWDRKSHELFISILKELEEHENWYAEVE"
     gene            130..>563
                     /locus_tag="LK694_002875"
     CDS             130..>563
                     /locus_tag="LK694_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053640.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYP"
BASE COUNT          233 a           80 c          119 g          131 t
ORIGIN      
        1 tcaggtatta cgatggagaa gacaaagaaa ggtctcttaa aacaatggga tcgcaaaagc
       61 cacgaattat ttatcagtat tttgaaagaa ttggaagaac acgagaattg gtatgcggag
      121 gtggagtaat tggctgacat caaaggactt gatatttttg ggaacaacaa aggactcgcc
      181 aaaataaaaa aagaagcaga agcaaagaaa aagcagcaag caattacacc atctgaaaat
      241 aagcaattag aagtgcacca agttcaagaa ataaaaactt cctccaatgt tgaaagacag
      301 agtagaggaa gaacaaaaaa agttggcgca ccaatcagaa agtttgataa ggtttattcc
      361 accaagcatc ctttaaaact gtcgactttg ttaaatgcga cttccagagt tttagtagaa
      421 aagtatgaga ctaatatgac aagagatgaa ttgcttagaa aagctttaga tgagtatatt
      481 aaacagaatc ttacgcaaga agataagcaa gatttgttta atgacgtggt gaaagaacta
      541 gacttgttca gagaaaaata tcc
//
LOCUS       DAFBQH010000198          555 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.207, whole genome shotgun sequence.
ACCESSION   DAFBQH010000198 DAFBQH010000000
VERSION     DAFBQH010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.207"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(271..534)
                     /locus_tag="LK694_002876"
     CDS             complement(271..534)
                     /locus_tag="LK694_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HBK5053641.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          215 a           97 c           75 g          168 t
ORIGIN      
        1 actctcctga attttttcat gttgaaaaca tcactaatca aaggcaaatt ataatttaga
       61 aatagaatga aaacactaaa tgaaaataga acattagaaa aaatagaatc aaaaaaaccc
      121 aagactagtg ccgaaataag ggaaacaatc gaagctacaa aaaataatga tttcgttgaa
      181 caggaccaat ttatttgttt ccgatatttc tcaaaaagaa ctttctttgg gaaagttaaa
      241 ggcgactgta aagtcgcctt tagtcctttt ttaccaagct gctgcatatt tagctccata
      301 ttttttaatt aatgatttgg ctgtagcaac aataccagct gtaattaatc cgcctccaac
      361 aactgcagca acaatagaaa tgattgtagc aaccgtgcta ccagtattaa ttatgtcaat
      421 aactttttta gctgcataag aagaaattcc aatgttagca cttagcattg gtacaccaac
      481 aaagaatccc gcaaaaccta aaattggtaa aactaataat aaattctttt tcataataat
      541 tcctctattt tttta
//
LOCUS       DAFBQH010000199          530 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.208, whole genome shotgun sequence.
ACCESSION   DAFBQH010000199 DAFBQH010000000
VERSION     DAFBQH010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.208"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..>530
                     /locus_tag="LK694_002877"
     CDS             <1..>530
                     /locus_tag="LK694_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK5053642.1"
                     /translation="LFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNE
                     FGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLF
                     KSITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFN
                     SVDEFFIQSVASKRNN"
BASE COUNT          184 a           88 c          101 g          157 t
ORIGIN      
        1 ggctatttga ttcttctgac ttaccgatga aaggcaagcg taaaccgaat ggacatcaag
       61 aaagacgtgg aaaacaaact ttccgccgct ctattcatga acgtgaaaag gattatagcc
      121 aattctcaaa tgagtttggt caccttgaag gtgacactat cgtaggtctg aaacataaaa
      181 gtgctgtaat taccttagtt gaacgattat caaaagttat catcacattg aaaccgtgtg
      241 gtagacaagc gattgatatt gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa
      301 acctattcaa atccatcact tttgattgtg gaaaggaatt ttcaaattgg aaacagatca
      361 gtaatgtcaa tgatattgcc atttatttcg ctgatccagg aacgccgtct caaagaggcc
      421 taaacgagaa ttctaacgga ttgttacgta gagatggttt attgaaatct atggatttca
      481 attcagtaga tgaatttttt attcaatctg tcgcatctaa acgaaataat
//
LOCUS       DAFBQH010000200          527 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.209, whole genome shotgun sequence.
ACCESSION   DAFBQH010000200 DAFBQH010000000
VERSION     DAFBQH010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.209"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            128..>527
                     /locus_tag="LK694_002878"
     CDS             128..>527
                     /locus_tag="LK694_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK5053643.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQT"
BASE COUNT          210 a           84 c           80 g          153 t
ORIGIN      
        1 atatcccata ttttaaaata aaagatagcg taaaatattt tgtgtaaata gaaatttagg
       61 gtagaaaaaa aagtccattc tgtaaaatta aagttaccac acagaaattt tataggagga
      121 ctttctcatg actaaattta ctatagaaat tatgcaaaca ctactcaata aaggggattt
      181 agatgaatta tttcaggatc atttagagcg agcgattaat tcacttctac aagcagagct
      241 aactgctttt ttagattacg aaaaatatga tcgaaatgga ttcaattcag gaaattcccg
      301 taacgggaat tattcacgta catttaaaac agaatatggc gagttgaatt taactattcc
      361 tagagatcgg aatggcgaat tttctcaaca aacactacca gcatataagc gaaccaatga
      421 ttcattagaa acgaccatta tccaattatt tcaaaaaggc attactatgg ctgaaatctc
      481 caaattaatt gaaaaaatgt atggtcacca ctatacgcca caaacaa
//
LOCUS       DAFBQH010000201          517 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.210, whole genome shotgun sequence.
ACCESSION   DAFBQH010000201 DAFBQH010000000
VERSION     DAFBQH010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.210"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..417
                     /locus_tag="LK694_002879"
     CDS             <1..417
                     /locus_tag="LK694_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK5053644.1"
                     /translation="TSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHVAG
                     WHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPS
                     KKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          179 a           94 c          101 g          143 t
ORIGIN      
        1 acttctgaaa acaagcctgt cattgatcca acacgagctg gtgcagctaa tcctacgctg
       61 acagatggaa caaatcacgc ccacattgat cagtttgggg aaatcgaaaa cgcaaacttg
      121 catgtggctg gatggcacat tgctaactat aaatacgagt atattttcat tatggactac
      181 aatactggga aagaattagc tcgagtaaga gctgatggaa tttatagacc agatgtaaac
      241 caagcttata atacttctgg aaatgttggt tatcatgtat ctttcaatat gcgtaatttt
      301 cctagtaaga aagtctatgt aatgatgcgg gcaacgaatg atccagaggg aaacactaaa
      361 ggcggtgcgc aagatttcca tgacaaacgt tggtatttaa atattcctaa acgataaaaa
      421 tagctcctcg ttgaggagca gtacataact atattgacaa ctataaaaat tattcgataa
      481 aatagtgatg ttatcgcata tcttcactat cacccat
//
LOCUS       DAFBQH010000202          498 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.211, whole genome shotgun sequence.
ACCESSION   DAFBQH010000202 DAFBQH010000000
VERSION     DAFBQH010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.211"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..451)
                     /locus_tag="LK694_002880"
     CDS             complement(<1..451)
                     /locus_tag="LK694_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053645.1"
                     /translation="MCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNSL
                     DFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKCFESAVVKFPQFKLNP
                     AASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKKAFKIK"
BASE COUNT          166 a           79 c           49 g          204 t
ORIGIN      
        1 ttttaatttt aaaggctttt tttatatctt ctaaattaga agatatttgt atatcaatat
       61 ggaatatatt accttttttg tattttttta tgttatcttt agctaaaaca tcaaatacat
      121 gtccttgaaa ttgtatcgag gcagctggat ttagtttaaa ttgaggaaac tttaccacag
      181 ctgattcaaa acacttgtca cattttttta tttttttacc tttgtaaaaa tattcgattg
      241 aatattctat ttggcacgac tcacttggca gtatatgtat gtcaatattt ttcatctgtt
      301 tcataggtat ttcaaaatct aaactattaa aaacatataa ttccctgtaa ttatttacat
      361 ctaactctac taaatttatc tttggaacaa tagatcctcc acccattcca ccataataaa
      421 aaaaatcttg ttctctatca aaatagcaca tattaagctc agtttgttta tcatcctcaa
      481 taattttata aatcaaac
//
LOCUS       DAFBQH010000203          488 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.212, whole genome shotgun sequence.
ACCESSION   DAFBQH010000203 DAFBQH010000000
VERSION     DAFBQH010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.212"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..414)
                     /locus_tag="LK694_002881"
     CDS             complement(<1..414)
                     /locus_tag="LK694_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053646.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFF"
BASE COUNT          185 a           78 c           62 g          163 t
ORIGIN      
        1 aaaaaacggc tttccaaatg atgaaatacc taaatctaac ttattaagaa agttgaaatt
       61 tttatattca ggaaaccatt tattaatttc atgtgcagca cgtaatgcat tccctggagt
      121 tgtcaaagag aaaattaatt ctgctaagct tattattgaa aatactgata acttataatt
      181 atatcgtcca ttgacaatca aatataaact aactatactt gttagaacaa aaaaacaggc
      241 gttgacttgt tcttgattag ttaaaaatat caataaagga atacttaccc aatatatttt
      301 tctatttatt tcttctcctc ttaagagttg aaaaaacgga taaaaagtta gtaaactaaa
      361 tgctactggc cactgatagt tcaaggtggt tgctctccaa ccggtttccc ccattactgt
      421 caatggtatt aaacagtata tagagcaaac caataataaa tttttaacat ttattttctc
      481 aaaaacat
//
LOCUS       DAFBQH010000204          487 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.denovo.213, whole genome shotgun sequence.
ACCESSION   DAFBQH010000204 DAFBQH010000000
VERSION     DAFBQH010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.denovo.213"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(<1..>487)
                     /locus_tag="LK694_002882"
     CDS             complement(<1..>487)
                     /locus_tag="LK694_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBK5053647.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DAFBQH010000205        20985 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.guided.042, whole genome shotgun sequence.
ACCESSION   DAFBQH010000205 DAFBQH010000000
VERSION     DAFBQH010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.guided.042"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(134..577)
                     /locus_tag="LK694_002883"
     CDS             complement(134..577)
                     /locus_tag="LK694_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBK5053648.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="LK694_002884"
     CDS             complement(795..2135)
                     /locus_tag="LK694_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5053649.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="LK694_002885"
     CDS             2404..3300
                     /locus_tag="LK694_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBK5053650.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="LK694_002886"
     CDS             complement(3450..4391)
                     /locus_tag="LK694_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBK5053651.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="LK694_002887"
     CDS             4517..5638
                     /locus_tag="LK694_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBK5053652.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="LK694_002888"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="LK694_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBK5053653.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="LK694_002889"
     CDS             7426..7992
                     /locus_tag="LK694_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK5053654.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="LK694_002890"
     CDS             8203..9264
                     /locus_tag="LK694_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBK5053655.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="LK694_002891"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="LK694_002891"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBK5053656.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="LK694_002892"
     CDS             10177..12159
                     /locus_tag="LK694_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK5053657.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="LK694_002893"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="LK694_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBK5053658.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="LK694_002894"
     CDS             12881..14305
                     /locus_tag="LK694_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK5053659.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="LK694_002895"
     CDS             complement(14486..15259)
                     /locus_tag="LK694_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBK5053660.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="LK694_002896"
     CDS             complement(15256..16161)
                     /locus_tag="LK694_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK5053661.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="LK694_002897"
     CDS             complement(16315..16635)
                     /locus_tag="LK694_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBK5053662.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17109..17795
                     /locus_tag="LK694_002898"
     CDS             17109..17795
                     /locus_tag="LK694_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBK5053663.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17797..18741
                     /locus_tag="LK694_002899"
     CDS             17797..18741
                     /locus_tag="LK694_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBK5053664.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            18940..19584
                     /gene="pcp"
                     /locus_tag="LK694_002900"
     CDS             18940..19584
                     /gene="pcp"
                     /locus_tag="LK694_002900"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBK5053665.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVSFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            19663..20940
                     /locus_tag="LK694_002901"
     CDS             19663..20940
                     /locus_tag="LK694_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053666.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6779 a         3783 c         4361 g         6062 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaaaaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttctata tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat
    16981 aatttttgtg ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa
    17041 acaactatat tttttatgtg ttttgcttta taattaaaaa aatattgtca ttttatggag
    17101 gaaaatatat ggaaaattat cttagccttt taggcgtctt gattgttatt ctcgggtttg
    17161 catttaaatt agactcgata ttgatcgtaa tggttgcttt agttgttaca gctttgactg
    17221 gcggtcttgg cattcaagga atgttagaac ttttaggtac aagttttgta aacaatcgcg
    17281 ggatggcgat ttttatcatc attatgttgg caacaggaac gcttgaaaga aatggtctga
    17341 aagaatcagc ggcttccttg atcaagcgat tcaagaaagt atctgctggt atgattgtag
    17401 atatttatgg gatctttcga atgatctttg cggcttttaa cgtgagtttt ggtggagttg
    17461 ctggatttgt tcgtccaatc attttaccga tggcattggg gaccatcgaa tcaaaagggt
    17521 tagaagtcca tcctaagcat gaagaagaac taaaaggtat ggcttcagct atggaaaatg
    17581 tctgctggtt ctttggccaa gtactattca tcggaggagc aggggcgttg ctagtccaat
    17641 caacactgaa agatctagga tatgacgtaa cattgggaca gcttgcttta gtggaagttc
    17701 cagtggctat cacggcttta gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat
    17761 tagcgaagaa atattatcca ggaaaggggc aataagatgg acttttttac aaatcctgat
    17821 attttattag gcgataaatt actggagatc ctttatatcg tgatgggact cgtattgatt
    17881 tacactggag taagaaattt aacagataaa accaatcctc atcgttatgg gtcagccttt
    17941 ttctggacgg tcttaggggt agttattgca ggaggaagat ggcttccggc aatcgtgaac
    18001 ggtggcttga tctttgcgat gaccatacca gcaattgcta agaaagtcag caaaggagaa
    18061 agccggcttc catcaaaaga atatatggaa aaaatgtcag acaaactagg aatgaaaatt
    18121 tttattccag ctttgagtat cggtgtcttt gcgatcttgt ttgctctatt taccaattta
    18181 ggtgctttag tcggcgtggg cgtcggtgtt tttgcagcca tgctaatctt gatgttcttt
    18241 tctcgagata atcgaccaag tacattttta gatgatgcag cagatatgct gggcacagta
    18301 gggccgttaa gcatgttgcc aatgctttta gcttctctag gtgcggtatt tacaagcgct
    18361 ggagttggga cagtgatttc caatgcagta ggtacgatcg ttccagaagg aaatgtgaac
    18421 atcggcatca tcatttacgc tcttggcatg gtgatattta cggtaatcat ggggaatgca
    18481 tttgcggcca tcaccgtgat gacggttggg atcggtgcac cattcgtatt tagtcatgga
    18541 gcgaatccag cgttaatagg aatggttgca ttgacttgcg gtttttgtgg gaccctgctg
    18601 acaccgatgg ctgctaactt caatatcgtg cctgtagcga tgctggagat gaaagacaaa
    18661 tacggagtga tcaaaaacca gttgtttatt gcgttattta tgctagtttt ccaaattgct
    18721 tatatgattc tatttaaata atagaagaga ggggttgtga cagaataatt gtcgcaacct
    18781 cttctaacat tgtagtggaa cactattttt ttataagaat atattaagtt tgatctgtta
    18841 tgttatttaa actattgttt cctttattta cgctgttcaa ggaaaatggt atgataaaag
    18901 ataaccatat gttaaatcaa tcggacggag gaacaacaga tgaaagtact agtaacaggt
    18961 tttgatccat ttggtgggga caaagtgaat ccagcctatg aagcagtaaa aaaaatgcca
    19021 gatgaaatca gcggtgctga aatcatcaaa gtggaagtac cgaccgtatt cgaaaaaagc
    19081 agtcaagtag tcaaagaagc catccaacag caccaaccag atgtcgtgat ctgtgtcgga
    19141 caagcaggtg ggcggtcagc agtctcgttc gaacgtgtcg caatcaatct agcagaagca
    19201 agaatccctg ataatgaagg aaatcagcca ttcgatacgg ctttagaaga aaacggtcca
    19261 gccgcttact ttacctcatt gccaatcaaa gcaatgacaa aaaatgtaca agatcatggt
    19321 ctaccagcgt acatctctta tacagcagga acatttgtct gcaatgatat catgtatcgt
    19381 ttgcttcatt tgatcgaaac acaattcccg gcaattcgag gtgggttcat ccacgtgtca
    19441 ttctcacccg atcaagtgat cgatcgtcca gtgggtacgc cttctatgtc tttagaagac
    19501 attgcttctt ctttgactta tgcagttgaa gcagcagtag aaaataaaga agatatttcc
    19561 ggaaatgccg gaacaactca ctaatttagc aagaaacact ggctggttgc taacgttatg
    19621 ttagtagcca gctttttgta attagaaagg aaagaaacag ccgtggaaga atggcaatcc
    19681 gtctttgaag aatggtttcc aaaagaaatc agcaaaagct atcccatcaa aatttctaaa
    19741 caatacacaa gcagtcaacg ttgggagatc tatgcaaaat taacgaaaaa acagcgggaa
    19801 ctagtcgata agcaccgccg atatctaatc agctctcgtt ttatggaaga acattattta
    19861 gctgcaacag attgggtgtt ttctgacttc aagatcaatc cgttttttcg aacaaaacga
    19921 agccagcaaa aattgtattg tgagtgtggt agagaattaa aagtgcaata catcgttaaa
    19981 tcgccaaaaa ccggaaagat cctaaaacta ggcatcaacc actttgccga tcacctgcat
    20041 gtctctccga cagtggcagc ttcgatccat cagggaatga caaaagttga tctggcctta
    20101 gacgaattat tatggcttaa gcagaagaat atcgattttc ctgaaggact atggcaaaaa
    20161 tactgttttg tcttgtacca aaatcgtcgg atgaaacagc catatttacc ggatataaaa
    20221 ttagctcagc ggctcgcaga atttcgccaa gtagaaatgc cgatttacat agcggattat
    20281 caagcattgg aaaatgaaat caagaaaata agtgaacaca tcaatgggca gtcaaaaaag
    20341 cgacagataa aaaaagaact atttgacgac tttgcagaag aattagtaaa ggatgtagaa
    20401 gaattcttga tcaactatcg cgcattttta cgtaaagatt ggcaatccat cgtatatgaa
    20461 gaagtgccag tacatcctaa tgcttatttt gaaacattta tttccgtgtt gagaaagaca
    20521 aaacgtcaac gaacaccaga agtcactgct cagatggaat attttgcgaa aaaccaacga
    20581 ttcatccaac caaaaatcta tctatttatt tggaagcaat attgtcgcta tggttttaca
    20641 gaagggttct tcgacagcat tcctcggata gtcagaaatg gttttttaaa agtattgcgt
    20701 aaagaacgag aagctataca atctgctgat aagaaagacc gtacagtctc aaaagaaaaa
    20761 tggcaactag tagtgaaaga tatccaatct ggaaatgtgc aagagacaat cgacaaatgg
    20821 aaaggaaaac attatcgttt tactgaagct cagaaacaag cgttggaata ttatcagaag
    20881 ttagaagaat cactgcgttt caatgatgag gcaagaaagt atctgaagga attactataa
    20941 aaaatagagg ctgggacaga agcgtttaac tccaagaaat aagaa
//
LOCUS       DAFBQH010000206        20863 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.guided.044, whole genome shotgun sequence.
ACCESSION   DAFBQH010000206 DAFBQH010000000
VERSION     DAFBQH010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.guided.044"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            267..1304
                     /locus_tag="LK694_002902"
     CDS             267..1304
                     /locus_tag="LK694_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBK5053667.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(1393..1716)
                     /locus_tag="LK694_002903"
     CDS             complement(1393..1716)
                     /locus_tag="LK694_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBK5053668.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(1703..2410)
                     /locus_tag="LK694_002904"
     CDS             complement(1703..2410)
                     /locus_tag="LK694_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBK5053669.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2496..2837
                     /locus_tag="LK694_002905"
     CDS             2496..2837
                     /locus_tag="LK694_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HBK5053670.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(3008..3856)
                     /locus_tag="LK694_002906"
     CDS             complement(3008..3856)
                     /locus_tag="LK694_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK5053671.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            4454..5317
                     /locus_tag="LK694_002907"
     CDS             4454..5317
                     /locus_tag="LK694_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBK5053672.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            5417..5899
                     /locus_tag="LK694_002908"
     CDS             5417..5899
                     /locus_tag="LK694_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBK5053673.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(6162..6629)
                     /locus_tag="LK694_002909"
     CDS             complement(6162..6629)
                     /locus_tag="LK694_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBK5053674.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            6752..7603
                     /locus_tag="LK694_002910"
     CDS             6752..7603
                     /locus_tag="LK694_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK5053675.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(7854..9812)
                     /locus_tag="LK694_002911"
     CDS             complement(7854..9812)
                     /locus_tag="LK694_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK5053676.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(9809..10681)
                     /locus_tag="LK694_002912"
     CDS             complement(9809..10681)
                     /locus_tag="LK694_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK5053677.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(11023..11895)
                     /locus_tag="LK694_002913"
     CDS             complement(11023..11895)
                     /locus_tag="LK694_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK5053678.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            12023..12592
                     /locus_tag="LK694_002914"
     CDS             12023..12592
                     /locus_tag="LK694_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBK5053679.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12659..13396
                     /locus_tag="LK694_002915"
     CDS             12659..13396
                     /locus_tag="LK694_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK5053680.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            13627..14895
                     /locus_tag="LK694_002916"
     CDS             13627..14895
                     /locus_tag="LK694_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBK5053681.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            15382..15846
                     /locus_tag="LK694_002917"
     CDS             15382..15846
                     /locus_tag="LK694_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK5053682.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            15830..16459
                     /locus_tag="LK694_002918"
     CDS             15830..16459
                     /locus_tag="LK694_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBK5053683.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            16813..18291
                     /gene="msr(C)"
                     /locus_tag="LK694_002919"
     CDS             16813..18291
                     /gene="msr(C)"
                     /locus_tag="LK694_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBK5053684.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            18338..18784
                     /locus_tag="LK694_002920"
     CDS             18338..18784
                     /locus_tag="LK694_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBK5053685.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(18983..19498)
                     /locus_tag="LK694_002921"
     CDS             complement(18983..19498)
                     /locus_tag="LK694_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK5053686.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            19615..20817
                     /locus_tag="LK694_002922"
     CDS             19615..20817
                     /locus_tag="LK694_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK5053687.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         6632 a         3703 c         4315 g         6213 t
ORIGIN      
        1 caaggagcaa tttcaaaaaa cagcttctca tgtttgatga atattttgac ttgtgaccga
       61 agagttggta ggagaacacc gtttataaga ggttgtgaca aaagtcttgt cgcaacctct
      121 tttttaacca aaaaagtgca aatagtttac tttaagtaag agaataatta tgatgaataa
      181 aaaaggtgga acaaatagca tactatatat attgaaatga acactgtatg gatgctccaa
      241 cttgatgaag ctaaggagga attcgtatgt cagaaaagga attaatgaag gtagtcggtt
      301 tttatgaagg gttgccgatc gatgatccaa aaagctttat tgatgaaaaa gaacagatac
      361 ctgtacccag ttcacgggat cttctggtaa aagtcaacgc agtatctgtt aatcctgtcg
      421 atacgaaact tcgccaagat aacgggataa gaaatgcttt acgtatctta ggttttgatg
      481 gagtcgggaa agttgttgct gtaggtgacg aagtgcaaaa attttctgtc ggcgatcgcg
      541 ttttttacgc aggtacaaca acaagagcag gaagcaatca agaataccaa ctagttgacg
      601 aacgtattgt cgctcttgca ccaaaaaatc tttcagatga agaagctgca gccttgccgt
      661 tgacctcatt gaccgcttat gaattgttat ttgaaaaatt tggtctaact ccagaagaaa
      721 atgccaatcg cgggaaaaaa atcttagtga tcaacggatc cgggggcgtc ggttcgattt
      781 tgaatcaact agcgcattgg gctggcctag aagtctacgc gactgcaagt ccgaagaatt
      841 ttgaatggct gaaaaaaaca ggtgtagact atccaatcga ttatcatcac gacttaagta
      901 aaagttttcg tgaattagga atcgatcaag tagagtatgt cgctgtatta ttcgatatta
      961 cccgctactt tgatcaaatc aagcaccttg tccgaccatt tggacatgtg ggaacgatcg
     1021 tagggatcaa agagccgtta gatataggtg tttggaaaaa ccaatctatc agcttcgact
     1081 gggagtatat gttcgctaaa acagactata actatgaaat cgaaacgcaa ggagaagctt
     1141 tagcgcacat tgctgcttta gcaaatgaag ggattatccg cagtacattg agcaaggtct
     1201 attcagatgg gatgaatgcg aagaacttga aacaagcaac caaagacgta gaaacaggac
     1261 atatgttagg aaaagtcgtt gttagtggtc cgttcaacgg ataagcaaga atacgccgat
     1321 ttttttctta tttgactttg ataaaaacaa gagcgcatga cagtaatttg ccataagctc
     1381 ttgttttttt atttataata agcgaatcaa cgaaagggaa agaattcctg ccaagacgat
     1441 caccaataag ctttttgaaa gaatagcagc taaaagtgta ggaactgaag ccaaagcatt
     1501 ttcgagattg atctgcggca gatgtccgat attttgggta aatagactgc tgaaccataa
     1561 agcagacata atgacgatcg ggacaaaact taaatactca agcaacggtt gcggtaaatc
     1621 gaattttttc agtaagacaa atggtaggac cctagaaagc caagttgcta aagtacaacc
     1681 taaaatagtc agcaatgtaa actcaaaaga aggcatgttt gatccacacc cccaatccgc
     1741 acccaatcag tgttacaact acaatcacta agttgcttgg gataaaaatc agtccgacat
     1801 ataccagaat caaagtgaga ccaatcagga tcaactgtaa tcgtttagag gtgtttctat
     1861 cagaaatgac ttgaagataa agtaacccga taaacatagc gacgatggcg aaatccaaac
     1921 caaacttttt cggatcagta ataaaattcc ctaaaagtgc gccaacaaga gaagaaccca
     1981 cccacgtcaa ataagaaatc caattagctg tattcatcca acgaaagttt aacttttgtc
     2041 cagtatagtt caatttattc attcccagtg cgaaactttc atctgtcagc aatgtgccaa
     2101 tcagcagatt ttgaagcagc cgatttttct tgaaataagg agcaatcgtc atactcatca
     2161 gaatcattcg ggaattgact agaaaggtcg agaaaacgat ggaaagcaat gggctgtgac
     2221 tagcaagcat gctgactgtg ataaattggg cagaaccagc atagaccagc agggacatca
     2281 acgtgacgac tagtggatga aatccggcag ctttcccaac gataccaaat gcaagtccaa
     2341 taccgatata accgaaaacg gtaggtaacg tgtctttgat cgcagttttt atatctaact
     2401 tttcatccaa taacaaaact cctttagcac gacataaatt ttcataattg tcgttaaact
     2461 tgtgaatatt ctaaattttt aatgagcatt tgtcaatggt gggattgaaa cgtaaagaac
     2521 tagcgggtcg cagatttaca cgtattgttt cggtgggagg aggcgcgaaa aatcttgcat
     2581 ggctacaaat ccaagctgat attttcgatg caacggtcta ttgtttaagt gtggaggaag
     2641 gacctgcatt aggcgcaaca atgattgctg cactagggtt agggtggttc gacacacctg
     2701 aaagttgttg tcagaatttt atccaatata cagatgcagc ggaacctctc ttacaaaatg
     2761 tagaaaagta tcataaaatc tatcaacaat gccggaaaat ctatccagca acgaaggaaa
     2821 tttgtcatga cttataaaac aaaacgcgta cctaatagtc aaatgactaa tattgggtac
     2881 gcgttaagtc ttctttactt catcctttgc atgattcgtg tttttaaagc aaggtctgct
     2941 gtatttcttg tggatttagg atggttgtta tcgtttactt gctatttttc attgaaatcg
     3001 tctttcttta ccgtttcttt agttttttat ctattttttt cttttggtta gaaaattctt
     3061 tttcccgaaa gtcttctttc aggattttta tagagtagcg ttttgaataa gaggcgtcag
     3121 tatgatcgac ttggcagacg aaccaagaga acatttgcct tttttctgca tgaggtgctt
     3181 ctgctaataa ttctttccat aaataataac agtcctctat cagaagaagg gttcgttcat
     3241 cctcaggctc ccaatagaat gtttcgagag tagcaatcac ttcattgatc aattcgaatg
     3301 cgggaagata ggcattttgc tgaataagcg attcaactgc ttcttggatg aactttcgca
     3361 tctccatgat gaacttcgct gttttcctgt ttttgttttt cttgataata tgatcgtatt
     3421 tatggatgat agttgttaat ttatctgaat aatttattgc atgaaatggc atcttaactt
     3481 tgaagcgttc gataaagtgc ggatgttcta taagaatctc tgttaaaagc tcccgtacaa
     3541 gagaggaact agcggaggaa acaagggacg agagttcgga aagtgagtag ttggattttt
     3601 ttattggacg ttcttcgtaa gcgaataaga cagcagccat gtgtttgcat ttttttcctt
     3661 ctatggcata tagacaatca caagtcatgt tggtcttttc atcccaagtg atcactacct
     3721 tataatcttc cgtgccagaa actgttgcgg acaagtactt gcttgtatag ttcaaatgac
     3781 ggacaaaacc gtgcagatga tagctgtaac cgcgttccaa aacgttttca ggaaaatact
     3841 caagccaatt attcatctag ttctcctttc atgaaacact tatttatatt tagtataaag
     3901 aaacgtttaa gaaaaaagta gctgatttta catatttgat aatcactttt gctcgaattg
     3961 tcaaaaattc attctccttt tcttttctta gagaatgtga gaaaaagtaa aagaatagcg
     4021 tgttttaaag atggaacttt ttttgttttt tatttatctg acaaaaatga agtgattttg
     4081 ttagaaataa ataattgttc caattgataa aagaggtatg atgtatcgca aagaaaaatc
     4141 caagcagcgt tctgatcagc agtttgaaca acaatttgct ttgtacagta ggtagtaagc
     4201 ggattaacta agggaataaa agatattttt accgctttgt ttacttagaa tctgtcttcc
     4261 ccaaaattta tttttctaaa ggtaaaacat agataggcag ataataccaa ttatccgtta
     4321 cgaaacgctg cttgagaatt ttctttagat ccatacaaca ttttctacat ctgactaaat
     4381 tggttcctga ttcatcaagt aattgacttt ctttgataat atgataaaaa ggcaatcaag
     4441 gaggaagata aatatgctta aagatctgaa ccaattgatc gattacatcg aagaacattt
     4501 gacagaagga ctttcctata aaataatagc cgaaaataca ggaatctctg aatatcaatt
     4561 aaaacgcatc tttacgttta ttgcagggat gtcgctaatg gactatatca aaaaccgtag
     4621 attggctttg gctaatcgcg atcttctaga tggatcaaga gtgacagaaa cggcgtttag
     4681 atatggatat gaaacagtcg aaggtttttc aagagctttt cgtgattggt caggatatct
     4741 gccttcagaa atcagtagat acggcataca aaaatctttt ccacgcttga cattttttat
     4801 tgatgtcaga ggaggaaaat cgatggaatt tcgtatcgaa aagaaagaac agttttatgt
     4861 ggcaggtgtg gcgaaaaaag ttcctgtcaa atttgaagga gtcaatcaag aaatacaaaa
     4921 gttagcgcag acgatcacgg agactcaaag ggaagagatg cataagttag gagatttata
     4981 tccaaatcaa gtaatcaacg tttcttatgg atttgatgga gaccgcttgg aagaaaaggg
     5041 cagtttgatg cacatgattg gctttgctac gacaaaagaa aatccgtacg ctgatttaga
     5101 aatgcttgct atagaagaac acacttgggc catttttcct aataaagggc catttcccca
     5161 aactttacaa gatacttggg ggaaaatcta tgcagaatgg cttccttctt cagattatgg
     5221 gttagtcgaa gctcccgaga tctcttttac aaaatgggga gaggatttca gtaatgttta
     5281 cagtgagatt tggattgcag tgaaagcaaa aaaatagtta aaaaataaaa atatccctag
     5341 aaaaaagagc gtaatcgtgt catttccctt taattttatg taagatgaac ttaatataat
     5401 agaaagggtg ggagaaatgg caaagtatag gatcgaagaa aaaccagatt ttacggtgac
     5461 gggtattggt gtggaattga agagtagcaa tacggatttt gtcggtttgg ctgcagagaa
     5521 acaagcattt gagcaaaaag ttatcggtaa tggaacaata gatgcattga aacaagctgc
     5581 tgaaaatcat tatttcttta cagtcaacga accctataac gggaaaatga tgtactatgt
     5641 cggtgttcaa acgagtcagg atgttccaaa cactacgcga atcatcccat tcccaaaagg
     5701 aacgtatctt gttgtaagcg ctttagcaga atcgcctgaa gaattaagaa atgatttgac
     5761 gaatacggcc tttgggcatg ttttaagaga agtagaagat tacgcctatg ttgggggtcc
     5821 aaatgctgta gtcgaaatgg gagttaccaa cgggaaatat atgggagaaa tctggatacc
     5881 ggtagtcaat aaaccatgat aatatagagg tcgtgaaagt agcgctttga cctgagcatt
     5941 agagaggaaa aatgggaaat agctttccat attttgcatt tttcagaact aatgccgagg
     6001 gtcagctact tgaacaccgt ttatcaagac gtcatgactt cagcgctttg acctgagcat
     6061 tagagaggaa aaaggggcat gacaaggctt ttgtcccccg gagaataaaa gaatttatgt
     6121 agacgattcc gatcagaaaa atgcaggcac agtagagaga attataaaaa tctctctacg
     6181 aggcctgtgt tttctggtct tttttctttt tccaaaacgg ctattttatg tatcagacta
     6241 tcaagtgtga tcttagccat ttcttcttct gcctttttct gcagccgagc gtagctttct
     6301 tctaaagctt cttggatatt cgcaccaacg acgcaatttg gatttgtttt tggatcgact
     6361 tggataagct gtgtattacc ttcgatgctg cgatagacat ctagtaaaga aatctgatct
     6421 ggacttttgg caagggaagg ctgcggtttt ccggtttttg taaagatcaa accggatttt
     6481 ttgagctgac tcatgatttt acgtacattt gctgcatttg tttcgatact actggcaatc
     6541 atctcactag aaagtgatcg ggtatcttga aacatttcaa tataggcaag tacatggatt
     6601 gcatcactaa attgcagaga atacttcatc agttgtcatc ctttcatttt ttttgcttga
     6661 cttatcttac cacactcttt atgataaagg tgtaatttat aaatgtacag ctgtaaatct
     6721 taacaaataa aaagaatagg gagtggcaaa tatgcattat ttagtaacag gagcaactgg
     6781 aggttttggt ggttatgcgc tggaattctt aaaaaagatg gttccacaaa atgaaatcta
     6841 tgtactagta cgtagtgaag aaaaaggaga agcactgaaa gaagctggat ttaatatacg
     6901 tattggtgat tatgatgatt tagactcaat gaaagaagcg ttgaaaggaa tagatcgatt
     6961 gctttttgtc tctggcgttc caggaaatcg tcaagccgaa cataagaatg tagtgaaggc
     7021 agcacaagca gctggggtat cttatatcgc ttatacaagt tttgcagatg ccgatcactc
     7081 gatcagtata ttagcgccag atcaccaatt cactgaaaaa atgattaagg attcaggtat
     7141 ttcccataca tttttacgga ataattggta tgtagagaat gaattaccta tcattgggca
     7201 ggctttaaaa actggtcaat tcgtttatgc tgcaggaaat ggaaaaacag gttgggcatt
     7261 gaaacgtgaa tatgcagaag ctgcagcaaa agtactagta gaaagtgact ctccagaaat
     7321 cttagaatta tccggcaatc taactgacta tgaagaatta gccaaggcat tagagcgtgc
     7381 gacaggtaaa gagttagaaa ttattgaagc aagtgatgca gcgtttgtcg aaaatctaaa
     7441 agaagccgga ttccctcaag aagcagctga catgttttta gcgatccaac atgatattaa
     7501 aaaggatcag ttagctattt cctctgatga tttagaaaaa gtattgggaa aaccattaac
     7561 ttctcttgaa gcagcattaa aagaaatatt ggatcaggaa taatgtataa agaggttgtg
     7621 aaaaagccga ttagttttga gtatgaagaa aaattttgtg aaaaagctgt tctgcatcaa
     7681 gtaataagaa gagtcaaata aaaaatagct cttcatatgt ttcgtttagc tggacttatt
     7741 accgcggagt tggcaggaga acgccgtttt tattcggaaa aaggagctga gacaagattt
     7801 ttgtctcagc tcctgtttgt ttatcgataa aaaagtctgg ggaagacgat ggctcaggct
     7861 ttgttggaaa cagagccgat tttatcattt gcttttacat cacgtgcggg ttcgatttct
     7921 aagctttgga ctttgtcgcc atttgtgaat acaacgacca tatccgtgtt tttgcctgca
     7981 gattgaatag cggcaagatc aactaaagca atcaattgac ctcttgcgac tttttgacct
     8041 tcttctacat ataaagtgaa tggttcgcct tttaagtcaa cagtgttgat gcccatatga
     8101 agcaagactt cgataccagc atctgtttgg attccgacag catgtttcgt tgggaaaatg
     8161 tttgtgatcg taccagcgat tggagagaag atttctccat tttctggtaa tacagcatag
     8221 ccgtcaccca tcattttttc cgcgaataca tcatctgaaa cttcagtgat tggaatcagt
     8281 ttcccatttg cgatagcgta aatatcttgt tcgatcgttt caccggcaaa tgtttctgtt
     8341 ttaaccacgg tgtttgtttg tgcaggtcgt gctgcagcac caccatgtag ttttgccatc
     8401 tcatctgcaa cgaattgtac ttctgtacct acgatgactt gcgcattttt tccgtcgatg
     8461 atcttcatac caggtacgcc agtggctttg attttatttt gatcgatcag gcctgtatct
     8521 ttgacttgca aacgaaggcg tgtagtacag ttatcgatcg aagttacatt gtcggctccg
     8581 cctaatgcag cgtagatttt gcttgcttgt gccgcatatt tattgtctga attagtcgtt
     8641 gcagtatcgt catctgaaag atctgcttcc tcgcggccag gagtcatcag atggaatttt
     8701 ttgatagcaa agttaaagcc aaagtagtaa atcactgctg ttactaatcc ttggacgatc
     8761 aacatcaatg gctgattcgc aatcggattt ttcaaagata ggaagtaatc gacaaatccg
     8821 gcactaaagg caaatcctgc tgtccaatgg aagaatgcac tcactgctaa agaaatccca
     8881 gtgaaaattg catgcaagac atataatggc caagcaacga acataaatga aaattcaagt
     8941 ggttctgtca caccagtaaa gaatgaagca aaagctgctg ctagcatcaa ggaagctgtt
     9001 tttgctttgt tttctggacg tgcatttctg taaatagcat atgctcctgc tggaagcccg
     9061 aacatcatga ttgggaaaaa tccagcttga tacattcctg ttacgccttt tgttccttca
     9121 gaagccaaaa atttaccaat atcattgatt cctgctacgt caaaccagaa cacggagttc
     9181 attgcttgat gcaatcctgt agggatcagt aaacggttga agaagccata aagacctgca
     9241 ccaccccagc ctaaactcaa aatgaattta gcaaaagaaa ctaaagcaga gtaaaccgga
     9301 ggccatacga gcagtagcac tgcactgatc agcaacatga caaaggcagc caagattggc
     9361 actagacgct tgccgctaaa gaaagataaa gccatcggta attttgcttc gtggaaacga
     9421 ttataaagag ctgccgcaat caatccggca ataataccaa taaatacgtt actgatctga
     9481 gcaaaagatg gatcaactga ttctggtttg atattcaata aagtagctac tgaatcagtt
     9541 tttaaaacat ttactggaac ttcaaatgcg accaaacctg ccaaggcagc agcacctgat
     9601 ttgtccactg acatccctag tgctaaccca acggcaaaca gcaaaggtaa ttgacctaaa
     9661 actgcgttac cgccagcttg taaaaaatga gcgacaatgt cgttactata agatgcccac
     9721 cagtttccga tccctactag taaagcagca gcaggtaaga ccgcaactgg aagttgaagg
     9781 gaacgtccca ttcgttgtag atatgctttc aatttttttc ctcctcatga ttgttaacat
     9841 ttgtttgtac gattgcgctt cttaaacgct cgatatggat ggccaagtaa cccagttctt
     9901 ctttcgtgat cgcgagctgg tattctttgg tcagcaacac acggattttt ttagaaatct
     9961 tatagctttc tgggtatttt cgtttgacca tttctacgat ttccgtgtct aaaacagcat
    10021 attgttgcgc atgggttcgt tgtagaagca gtcgtaaatg attcaccagt cttgaataat
    10081 tcagtgaaaa tggttctgaa tggatatctg tttccaattc tgattccact aaatggatga
    10141 tgtcagatac aatcgtcact tcacggatac tgcgatggtt attgttccga ccgcttcgtc
    10201 cgctgtgaat atggattgcg atatatcctg cttcgtcata agtatatgga atcccaagct
    10261 cttttgtcag atactctact gcccattgag caatactgaa ttcatcactg taaagcactt
    10321 cgatttctct taaaagtttg ttgcggatga cgataccgtt attaatgttc tccgcagaaa
    10381 aagcaatgtg gtcggtcaaa gcgatcaata catgttcatt caacttttcc atcaaaacag
    10441 tttccgcatg atcaatgatc gtttctgctg caaataaaaa gcgttcatca atctgattaa
    10501 gtaaattttg caacttgatt tgaccttctg gttccatgac aaatatccgt tcgacatcac
    10561 gatcaaaaat caaatcattt ctttttctgt caaatccaat cccttttcca atagctactt
    10621 tttcctgtcc accgtcatct accaatactg cattttgatt aaagatcttt ttgattttca
    10681 tgcgcgcacc ctttttgaag atttggtttt cacgtttgct agaccagttg attgcggcca
    10741 aacaaaaata gacacgaaaa acctcctagg gaaaccatgt tcctcagaga tttttcatgc
    10801 ctagtatgat ctagtcacat gaaacgaatt attgttaacg taatcaattt tatatagaca
    10861 gttatgtttt gtcaattctt tttttataat tatagtaatt tactggaatt atcagaaata
    10921 aaaggaagaa ataaattaag aaacttatgt tatttaatcg ttggatcttt tgcttatctt
    10981 attcgttgtt aaaaagctga aagcgaagga aaaaaactct acttaactgt atttctccgt
    11041 taggacatct aaaaatttac gagcagcagg agaaagaggc tggtcttttt tccaaatgag
    11101 attcagtgaa gcttgtaatg gcggatcgag tgggatgaat attttttccg tcccataagt
    11161 attgatgatc ccatctagag ctaatgcacc aaaagtcccg ttatcgacca tcaaagaagc
    11221 attgtacaat aagttatatg ttccgacgat cgataactga tctatgctga aacctagcca
    11281 ttcagctagt tgactgtcca ctgacgtttg ccgggaaatg atcaaaggaa cagttttcag
    11341 ttcctgcgga tggatgacgg tttgctttgc taaaggatga tttcgatgta tcaaaagtcc
    11401 ccaacgatct tgggctggca gtcgaaatga ctggtatttt tgtttgtttg tcggttcgat
    11461 tacgagtcca aaatcaagta atccgctatc cagtttgtct atgatatcat ctgcattccc
    11521 gctatataaa tgaaactgga tatcaggatg cgccttgatc aacgaatgaa ggctatcaga
    11581 aatgaaagag attgctttag tttcgcctcc gccaatgtag atttctccag agatgattgc
    11641 gtcatttgtt aaattgttcg ccgttttttc taccaacgac aagatttctt ttccttttga
    11701 ataaagatat cgaccagaat ccgtcaactg tatctctcgg gagccccgag tgaaaagtgt
    11761 cacaccaagt tcttcttcta aatctttcag ttgttttgag agagtaggtt gcgtgatatg
    11821 cagagatttt gctgctcggc tgatattttt ttctctagct actgctaaaa aatacgtcag
    11881 tatacgaata tccatttttt ccctcctgtt tttaaaaaag cataccacta agtatagtta
    11941 aaaggaatag ttaagtatgt tttataagta ttagacatga taagccgtcc gttataaagt
    12001 aaggataaaa ggagggagca aaatgacaag cctcatcgaa aaacaaatcg tcaataaaga
    12061 gattcgagta acggatccgc tctttgaagt gatccatcag atccaagctg aaaacgaaga
    12121 gcctttagca gcgctgaata ctgggtacca tgaaccagca gatatccgca aacggctaga
    12181 aaatatcatt agtgacaaag tagatgatac ggtgactgtt ctgctgcctt tttatacaga
    12241 tttcggcaaa catattagta tagggaaaaa tgtctttatc aatcgtcaag tgatgttcgt
    12301 tgacttaggt ggcatctgtt tagaagattc agtattgatt ggtcctcgtg tcaacctcat
    12361 cacggtgaac catctaaccg atccaaaaga acgaagaggc ttgtcagtaa agccaatcca
    12421 catcaagaaa aatgcttgga tcggagcagg agcaactatt ctgccaggcg taacgattgg
    12481 cgagaatgcc attgttgcag cgaatgcaac tgttacgaaa gatgtcccag acaataccat
    12541 cgttgcagga atccctgcca aaatcgtcaa acctgtcgaa cggattattt gaacagtaag
    12601 atgaaataag agatagtgag tataagcaag agatttttca aaacaggagg agaatagaat
    12661 gaatgattta tcagaaaaag tgattgtcat catgggcgct tcaagcggaa tcggtgaagc
    12721 aacaacgaag ctattagcag aaaaaggggc taaactagtt atagctgcac gccgagaaga
    12781 tcgcctaaaa gcaataaaag aatctttacc agaagctgag ctatatattc aaacagcaga
    12841 tgtaagagat tttgcacaag tccaagcagt tatcgattta gcgatggaaa agttcggtcg
    12901 aatcgatgta ttgtacaata atgcaggaat catgccaacc gctccattag ttgaaggtca
    12961 ccgtgatgaa tggcaaaata tgcttgatat caacatcatg ggtgtgttaa atggaatttc
    13021 cgccgtactt ccaatcatgg aaaaacaaaa atctgggcat atcatttcaa ctgattctgt
    13081 ggcgggacat gttgtttatc ctgattccgc cgtttattgc ggaacaaaat ttgctgtccg
    13141 tgcaatcatg gaaggattac gtcaagagca acggcaaaat aacatcaaat ctacgatcat
    13201 ctctccagga gctgttcaaa ctgaattata tcaaaccatc agcgacaaaa aggctgctct
    13261 tgaacttcat gaagcgcaaa aagaatgggg attgacctcc gaagatatag cttctgctgt
    13321 agcatttgcg atagaaacgc cagaccggat gtctgtcagt aacatgatta tccgtccgac
    13381 gacacaggaa gtataatcca taacggataa tatccaaaga gaattacttg ttaaatagtt
    13441 attcaaacga ccatcaattt aaggcgtgaa tggagaagaa tattcttcat ttacgtcttt
    13501 ttatgtagaa aaagcaaatt tatcaaaaag aaacaaatgt tatttaaata tggacattga
    13561 cttgctttta cttttttagt tacactgtta tttaggaaaa gtttcataat tcaaaggaga
    13621 aaaaagatga aagcaaagaa tttattattt agcgttattc ttttaggaaa tctagcagtt
    13681 gccaacacaa cagtgctagc tgaagaatcg acagaaactg tcgcaactca gacatcgagt
    13741 tcttcagaaa caggaattga aaacgataca gagagtactt tgcagtcagc tactgtctca
    13801 gaaaatacaa tttcaaccga agaaccaaca actgaaacaa cagaacaaac acaaaccgaa
    13861 gacagtacat cagcagaagc tcagaacaat ccagagaatg cagaagaaat cacgaaccaa
    13921 ttgacagatg ggcaaacatt gacacatacc catcaagcag atttgtctga attcgaaaac
    13981 tatactggct atgcttttta tgctcgctca tctgctaatt ctcaacaagc gttcattgat
    14041 agtattgcct caactgcaca aagtttggca agtgccaatg acttatatgc atcagtcatg
    14101 attgctcaag ccatcgttga aagtggctgg gggaacagta ctttggcttc tgcccctaat
    14161 tacaatctat ttgggattaa aggaagctat aatggccaat ctgttacgat gcctacttcg
    14221 gaatatgtga atgggcaatg gattacggtg aatgccgctt ttagaaaata tccttcgtac
    14281 aaagaatcat tgcaagataa cgtaacggta ttgaagacga cttcttttca gccaggcgtt
    14341 tactactatt ccggtgcatg gaaaagcaat acgaactctt acaaagatgc tacggcttgg
    14401 ctaacaggtc gttatgccac tgcgcctaat tatggttcta cattgaacaa tgtaatcgaa
    14461 acctataatc tgacacagta tgatacagca ccaacttcaa cttctccgat gtatcgtctg
    14521 tataaccgtc atacaggaga acatttatat acattgaatg ccggagaaaa aaattatctg
    14581 ccaactgtag gctgggaata tgaagggatt gcttggcaag caccaaacag cggacaacct
    14641 gtctaccgtt tatacaatcc ttacagtgga gaccatcatt acacaatggc acaatctgag
    14701 atcaactctt taacaaaaat cggttggcgt tatgaaggtc taagttttta ttctggtggt
    14761 tccaagccaa tttatcgact atttaatcca aatgaaaaaa ctggtaccca tcactatacc
    14821 ttgagtgcta aggaaagaga ttggctaaca ccgatgggct ggagatatga aggaatagga
    14881 ttttatggtt attaaaaaga ggtagtgaaa aagctgccct gcatcaagta gtaagaacag
    14941 ccaaacaaaa acagattttc atgttttttg tttggttgaa cttattaccg cagatgcaag
    15001 tttttgaaca ccgtttactc aaaaaaggtt gtgacaacat cttgtcataa cttttttatt
    15061 actgtttaag catactgatc caatttctaa tattcgacgg gattcttgca aaaggcgatt
    15121 tcggaaattt ttggcggtgt aaaattttct actgcagaag agctatcttc tatagtttga
    15181 taagaatcaa taatcttggt tttagcgttg tgctttcttt atcatttttt ccgttcattc
    15241 tacaaaaaat ttcttttctc tatacttttc tattgagagc gtttttttta ttacacagct
    15301 gattctttga cttttttctt tttttattct attattgaca agtcaatcgt tgacgagtca
    15361 attattagaa aggaggaaat tatgtcttta gaagagatta gtcacctttt atatcaaatc
    15421 aaattagccg atcaaaaaat cgtccagatc tttgaagaaa atatcggtat aagtcttact
    15481 agatacgaga tcatgatgag actgaaagaa gaaaatccat tgctacaaag cagtttgcaa
    15541 gaaaagttgc agattgatca aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat
    15601 ggctatgttt cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt agcgctaaca
    15661 gaaaaagccc agaagaaaat cgagcattgt gaaatggacc ataaagatac aggttccgtg
    15721 ctatccccgg ctttcagcga tgtagaaatg aaacaatttc taacactttt agataaattg
    15781 aatacccaac tttgcaatat aacaacagaa acgaaagagg ttaataataa tggaacaaaa
    15841 aaatgatttt tcaacaatta tgactggtag aagatccgtt cgcgtatatg atgaaacata
    15901 caaaatccca cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg caccttcttc
    15961 tgtcaatatg caaccatggc gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt
    16021 gcgtcctttg atccgtttca atacgagaca aaatgatact tcttctgcaa tggtcatgat
    16081 ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc tataaccaag cttacgaatc
    16141 agggaagatg ccaaaagaag tcagagatca acaattagct gcgatcattc cttattacaa
    16201 gagtttttca agagaagaaa tgaacgatgt agtcaaagtc gattctagct tagcggctat
    16261 gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg gtggattcga
    16321 agcagatcag ttggcagagg catttggttt ggataaagac cgttatgttc cagttattat
    16381 tttatcaata ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag caccagaaaa
    16441 gatcactacg ttcgaataaa aggttttcag ttactattca gtagctattg ctttttttga
    16501 atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta
    16561 cgtttcgaac ttttgaataa caactaaata aattcaaaag acatgtataa acaacaattt
    16621 catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga
    16681 tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt
    16741 tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg
    16801 agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc
    16861 aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc
    16921 ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt
    16981 cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat
    17041 ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa
    17101 gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt
    17161 ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa
    17221 agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt
    17281 catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa
    17341 atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag
    17401 actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc
    17461 caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa
    17521 caaatcagac cggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc
    17581 caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg
    17641 cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat
    17701 cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt
    17761 gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag
    17821 acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta
    17881 agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt
    17941 tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta
    18001 gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc
    18061 cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg
    18121 actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg
    18181 ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt
    18241 tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac
    18301 gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg
    18361 aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt
    18421 atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt
    18481 ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa
    18541 actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt
    18601 tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga
    18661 tcaattattc tctgcttata tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt
    18721 gcagttgatc atcgttggcc cgctttcaag aagtgtgcta aaagcaattc aaagtaatga
    18781 atgatggaac actgttattt gaaaaaaggc cttggcatgc tttttgtcat agtctcttga
    18841 atgcagttct agaaaaatag tgatccgaac gataggtttt ggcctgtcgt tcggatcgct
    18901 ttttcttttc caactgtatt gctcttttat tgaagataat ttgctcttca atagatattt
    18961 ctagaaaggt tctgtttatc ccttactcaa aaaactgctg catttgttct cttacttgct
    19021 gacaccattg gagttcctgc tgacaaagca taattctgcg ctggatcaca acataacgtc
    19081 caaattcttg tttcttgtct agcgagaggt cattcaattt tttttgattt ttttgcatga
    19141 ttttgttcag ctgttgttct ctagtgaata gcagtccagt agctgttgtt ttatcgagcg
    19201 tcgaaaaagc atagattttt gctagaaatt catctttttg cgtaggagcg ggagtttcct
    19261 ctttgatcca ttcctctaca agagcacgtc cttcatcagt caagtggtag atcttttttt
    19321 gagagtgctc gtctttgtcc aaaacggtca cataaccctg ctggtctaac ttggcaagcg
    19381 ttgtatagat ctgactgtga tgggcttccc aaaaaattgt gatcagctgc ttcaattcat
    19441 acccagaacg tggtttggat aaaagcacgc ataataatat ataggctaaa gtattcataa
    19501 aaatcctacc tctcgtctta ttttctttat tatatcacag taaagtttcc acgttttaca
    19561 tgtgcatatt tacatgtaaa ataaatcatg tatgatgcta atagagagaa agggatggaa
    19621 atattggaga atcaaataga tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt
    19681 tcagcgctag ttgtagcagc tacctgtatg atcgctttta tgggagtggg tctagtagac
    19741 ccaatcttaa aaacgattgc tgcacagtta aacgctacgc cagctgaaac gaccctgtta
    19801 tttactagtt acatgttagt cactggtgtg gtgatgctat ttacaggttt cttatctagt
    19861 cggatcggtt tgaaaaaaac attgatgaca ggattgttta tcatcgttgt attcgctggt
    19921 cttggcggat tatctgggaa tatcggaatg ctgattgggt tacgtgccgg ctggggtgtc
    19981 gggaatgctt tatttgtctc tactgcttta gcaacgatcg tcagtatttt agttggtaac
    20041 acagaaaaag caatcatgat gtatgaagca gcacttggct gcggaatggc tgttggccct
    20101 ctagtcggtg gggtcttggg cagtggttca tggcgttatc cattttttgg ggtagcattt
    20161 ttgatgtttt gcggttttat agccctagcg gtacttttac ctgaaactga gaaaccaaag
    20221 agaaaagtag gcctttggga aggtgtcaaa gcgttaagca atcataaact tcgttcagtt
    20281 ggactaatcg cattgttata taatttcgga ttctttactt tacttgcgta tgcgccattt
    20341 ttattgattg gctattctga attagaagtc ggttttgtat tttttggctg gggtgtgctt
    20401 ctggctattg cttcgatttt cgttgcaccg gctcttgagc ggaagtttac gacttatcgg
    20461 acaattttag cagcatttgt cttattcatt atttgtttgc tgttattagg agctggaact
    20521 tctatgccag tccttgtacc gatcagcatt gtgttagcag gatttttcca aggaattatc
    20581 aatacactgt tgacgacgat tgctatggaa ataccaggtc tgcaacgaaa cgttgcttcg
    20641 tccagctata gttttgtccg ctttttcggt ggagctttag caccatttat tgcaggtgtg
    20701 attggtgaac atattggtga aagctatgcg ttttattttg caggtgccat tgttcttatc
    20761 agtgtatgtt gtatcattta tcaccgtaat tattttgcta caaatgaaga agaataagaa
    20821 atcagaggtt gtgaaaaagc tgtcctgcat caagtcataa gaa
//
LOCUS       DAFBQH010000207        11427 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.guided.085, whole genome shotgun sequence.
ACCESSION   DAFBQH010000207 DAFBQH010000000
VERSION     DAFBQH010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.guided.085"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            39..977
                     /locus_tag="LK694_002923"
                     /pseudo
     CDS             39..977
                     /locus_tag="LK694_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            980..3163
                     /locus_tag="LK694_002924"
     CDS             980..3163
                     /locus_tag="LK694_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBK5053688.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3160..4161
                     /locus_tag="LK694_002925"
     CDS             3160..4161
                     /locus_tag="LK694_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBK5053689.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4176..5090
                     /locus_tag="LK694_002926"
     CDS             4176..5090
                     /locus_tag="LK694_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK5053690.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5335..5451
                     /locus_tag="LK694_002927"
     CDS             5335..5451
                     /locus_tag="LK694_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBK5053691.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5467..7386
                     /gene="tet(M)"
                     /locus_tag="LK694_002928"
     CDS             5467..7386
                     /gene="tet(M)"
                     /locus_tag="LK694_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBK5053692.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7484..8857
                     /gene="tet(L)"
                     /locus_tag="LK694_002929"
     CDS             7484..8857
                     /gene="tet(L)"
                     /locus_tag="LK694_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBK5053693.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9421..10683
                     /locus_tag="LK694_002930"
     CDS             9421..10683
                     /locus_tag="LK694_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBK5053694.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10928..11389
                     /locus_tag="LK694_002931"
                     /pseudo
     CDS             10928..11389
                     /locus_tag="LK694_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
BASE COUNT         3772 a         1910 c         2388 g         3357 t
ORIGIN      
        1 acttggagat aacatcaaaa agttacctat cgctataacg tggcagatgg aaagaaacct
       61 tgccagagat ttcccatgaa atcaatatcg tcaatctgac ttctgatgag aaaaacaaag
      121 gcttacttga cccttatgta attatgaaaa ataccaaaga ttctgaatca ctggctattg
      181 atattttgac attccttacg gggatttcct ctcgtgatgg ggaacgcttc ccaatcctta
      241 gaaaagccat tcgtgcagta accaatagtg aagtgcgagg gttgatgaaa gtgattgaag
      301 aattacgggt tgagaatacg ccactaagta ccagcatagc cgaccatatc gaaagtttta
      361 cagactatga ctttgcccat ctgcttttta gtgatggtta tgtagagcag tctatcagcc
      421 ttgaaaaaca actgaacatt atacaggttg ccgacttggt acttcctgac aaggaaactt
      481 cctttgagga atataccaca atggagttac tatcggtagc aatgctgatt gtcattagta
      541 cctttgcgtt ggactttatc catacagacc gaagtatttt caagattgta gacttagacg
      601 aagcatggag cttcttacag gtggcacaag ggaaaacact atctatgaag ctggttcgtg
      661 ctggtcgtgc tatgaacgct ggggtatatt tcgtgaccca aaatacagat gacctcttag
      721 atgaaaaact gaaaaataac ctcggcttaa aatttgcctt tcgttccact gaccttaacg
      781 agattaaaaa aacccttgcc ttttttggtg tagacccaga ggacgaaaac aatcagaagc
      841 gattgcgtga tttggaaaac gggcaatgcc ttatcagtga tttatatggt cgtgtcggtg
      901 tgatacagtt ccaccctgta tttgaagaac tgctccatgc ctttgatacc agaccacctg
      961 tgcgaaaaga ggtgtaaatg tgaaaccatc aatattaaac agaataaaat caaactggac
     1021 gctgaaacgt ctaggtaaag tggcaatgac agtggctttc acacttgtga ttgccatttt
     1081 tcttttagcc atgctgggaa cggtggttca agctgcgggc ttggtagatg atacggtcaa
     1141 tgtggcaaat gaatacagcc gatacccact tgaaaactat caactggatt tttatgtgga
     1201 taatagctgg ggctggctac cgtggaactg gtcggacgga attggaaaac aagtcatgta
     1261 tggactatat gccattacca attttatttg gacaatcagt ctttatgttt ccaatgcgac
     1321 gggttactta gtacaggaag cctattcctt agacttcatt tccgctacag cagattccat
     1381 tggcaagaat atgcagaccc ttgcaggggt tacgcctagt ggactatcaa cagagggttt
     1441 ctatgttgga ttccttttac tcttgatttt ggttcttggg gtttatgttg cctatacagg
     1501 actgataaag agagaaacca caaaggcaat tcatgccctc atgaattttg tgctggtttt
     1561 tatcctatcg gcttccttta ttgcctacgc tcccgactac attaaaaaaa tcaatgactt
     1621 ttcatcagac atcagcaacg ctagtttgtc actgggtaca aaaattgtca tgccccattc
     1681 agatagtcaa ggcaaggaca gtgtggactt gatacgtgac agcctatttt ccatacaggt
     1741 tcagcaaccg tggctactgc ttcaatataa cagttcagac attgaaagta ttggtattga
     1801 ccgtgtggaa agcctgctct caaccagtcc agattccaac aatggggaag acagagaaaa
     1861 aattgttgcg gaagaaattg aagatagaag caataccaat atgacgatta caaagacgat
     1921 taaccgttta ggtacagtct tcttcctatt tgtcttcaat attgggattt ccatatttgt
     1981 attcctatta acaggaatca tgattttctc gcaggtactt tttatcatct atgctatgtt
     2041 tctgcctgtg agctttattt taagcatgat tccatcattt gatggtatgt caaaacgagc
     2101 cataacaaag ctctttaata ccattttgac acgagctgga atcacattga ttattacgac
     2161 agcatttagt atttcaacca tgctctatac cttatcggct ggttatccgt tctttttgat
     2221 tgcttttcta cagattgtga cctttgcagg aatctacttt aagctgggcg atttaatgag
     2281 tatgttttct ctacagagta acgattctca aagtgtggga agtcgtatga tgagaaaacc
     2341 tcgtatgctt atgcacgctc acatgcaccg tctacagcgg aaacttggac gctccgtgac
     2401 tgctttaggg gctgggtctg ccattgccag tgctacgggt aaaaaaggac agtcgggttc
     2461 gggaagttct gcgagtacac aagcagatca ctctcgacca aacgggcagg aaaaatcaac
     2521 actaggaaag cgtatcggtc aaaccatcgg tacagtagct gataccaaag acagaatggt
     2581 agatactgct ggtaatttga aagaacaggt taaggatttg ccgaccaatg caagatatgc
     2641 agtatatcaa ggaaaatcta aagtgacgga taatgtccat gatttaacca gaagtatttc
     2701 tcaaaccaaa gcggataaag ccagtggacg caagaaacag caggaacaaa gacggaaaac
     2761 cattgcggag cgtcgctctg aaatggaaca ggtcaaacag aaaaaacagc cagcttcttc
     2821 cgttcatgaa agaccagcca ctaagcaaga acaatctcat gatggacaga cttcaaggca
     2881 aacaactgta caggcttcct atagggaatc tcaacaagcc aaacaagagc gtccaacagt
     2941 taagtccgat tcttcaagtt taaaaacgga acgtcaaagt aatacagttc aagaaagaac
     3001 ggttcaaaag ccagtaactt caactgcacc aacagataga gcttcacaac gttcaatcac
     3061 aaaagaacgt cagtctactg ttcaaagagt accactacaa aatacaaaaa gtagaccacc
     3121 aatcaaaacc gccaccatta agaaaggcag taagaaacca tgaagctgaa aactttagtg
     3181 attggtggtt cgggattatt cttgatggtc ttctcactgc ttctgtttgt cgccatttta
     3241 ttttcagatg aacaagatgg cggtttttcc aacattcact atggcggtgt ggatgtttcc
     3301 gcagaagtgc tggctcataa gcctatggta gaaaaagttg ccaaagaata tggaattgaa
     3361 gaatacgtca atatacttct tgcgattata caggtggaat cgggtggtac agcagaagat
     3421 gttatgcagt cctcggaatc cctcggtctt ccacctaatt cattgagtac agaagaatcc
     3481 attaagcaag gtgtgaagta tttcagtgag ttattagcca gtagcgaaag gcttggtgta
     3541 gatttagaat cggttatcca gtcctacaat tatggtggtg gtttcttagg atatgtggct
     3601 aatcgtggga ataaatatac ctttgaactg gctcaaagtt tctcaaaaga gtattcgggt
     3661 gacgaaaaag tgtcttaccc caatcccata gccataccta tcaatggggg ctggcgatac
     3721 aattacggga atatgtttta tgtgcaactg gtaacgcagt atcttgtcac aacagagttt
     3781 gatgatgata cagtacaagc catcatggac gaagctctga aatttgaagg ctggaagtat
     3841 gtctatggcg gagcttcccc gactacctct tttgattgta gcggactgac acaatggacg
     3901 tatggaaaag ctggaatcaa cttaccacga acagcacaac agcaatatga tgtaacccgg
     3961 catatcccat tattggaagc aaaagccggc gatttggttt tctttcattc tacctataac
     4021 gctggctctt atattactca tatcgggatt tatttaggcg ataaccgtat gtttcatgca
     4081 ggcgacccta tcggttatgc cgacttaaca agctcttact ggcaacagca tttagtggga
     4141 gcaggacgaa tcaaacaata agaaaggacg attcaatgat gaaattcgga aaaaatcaga
     4201 atacagaaaa acagacacca aaggaaaaga aacctcgtgt ctataaggtc aatcctcgta
     4261 aaaaggttgt gattgtccta tgggtacttt taggacttag tttcagcttt gcgatattca
     4321 agcactttac agccattgat acccatacca ttcatgaaac caccatcata gaaaaggaat
     4381 atgtcgatac tcatcatgtg gaaaattttg tagagaactt tgcgaaagtc tactattcat
     4441 gggaactaaa cgacaagtcc attgataatc gcatagaaaa cctgaaagca tatctgacag
     4501 aggaacttca agctctgaat gtcgatacag ttcgtaaaga tattcctgta tcgtcttctg
     4561 taaaaggatt tcagatatgg acggtagaag cagatggcga caatcaattt gatgtaacct
     4621 acagtgtaga ccaactcatt acagaggggg aaaatacaaa gaccgtccac tctgcttata
     4681 tagtgagtgt ctatgtagac agtactggaa atatggtcct cattaagaat ccgaccatta
     4741 ctagcatacc aaagaaatca gactataaac caaaagctat tgaaagtgag ggtacggttg
     4801 attccattac aaccaatgaa atcaatgagt ttttaacgac gttcttcaag ctctatccta
     4861 cagcgacagc cagtgaactt tcctactatg tgaatgacgg gatattaaaa ccaatcggaa
     4921 aagagtacat ctttcaagaa ctggtaaatc ctatttacaa tcgtaaggat aatcaagtca
     4981 cggtatcgct gacagtggag tatatcgacc agcagaccaa agcaacgcag gtatctcaat
     5041 ttgatttggt acttgaaaag aacgggagta attggaagat tatagaataa caaatattgg
     5101 tacattatta cagctatttt gtaatcacgt actctctttg ataaaaaatt ggagattcct
     5161 ttacaaatat gctcttacgt gctattattt aagtatctat ttaaaaggag ttaataaata
     5221 tgcggcaagg tattcttaaa taaactgtca atttgatagt gggaacaaat aattggatgt
     5281 ccttttttag gagggcttag ttttttgcac ccagtttaag aataccttta tcatgtgatt
     5341 ctaaagtatc cggagaatat ctgtatgctt tgtatgccta tggttatgca taaaaatccc
     5401 agtgataaga gtatttatca ctgggatttt tatgcccttt tgggcttttg aatggaggaa
     5461 aatcacatga aaattattaa tattggagtt ttagctcatg ttgatgcggg aaaaactacc
     5521 ttaacagaaa gcttattata taacagtgga gcgattacag aattaggaag cgtggacaaa
     5581 ggtacaacga ggacggataa tacgctttta gaacgtcaga gaggaattac aattcagaca
     5641 ggaataacct cttttcagtg ggaaaatacg aaggtgaaca tcatagacac gccaggacat
     5701 atggatttct tagcagaagt atatcgttca ttatcagttt tagatggggc aattctactg
     5761 atttctgcaa aagatggcgt acaagcacaa actcgtatat tatttcatgc acttaggaaa
     5821 atggggattc ccacaatctt ttttatcaat aagattgacc aaaatggaat tgatttatca
     5881 acggtttatc aggatattaa agagaaactt tctgccgaaa ttgtaatcaa acagaaggta
     5941 gaactgtatc ctaatatgtg tgtgacgaac tttaccgaat ctgaacaatg ggatacggta
     6001 atagagggaa acgatgacct tttagagaaa tatatgtccg gtaaatcatt agaagcattg
     6061 gaactcgaac aagaggaaag cataagattt cagaattgct ccttgtaccc tgtttatcat
     6121 ggaagcgcaa aaagcaacat agggattgag cagcttatag aagtgataac gaataaattt
     6181 tattcatcaa catacagaaa gaagtctgaa ctttgcggaa atgtcttcaa aattgaatat
     6241 tcggaagaaa gacaacgtct tgcatatgta cgcctttatg gcggaatcct gcatttgcgg
     6301 gattcggtta gaatatcgga aaaggaaaaa ataaaaatta cagaaatgta tacttcaata
     6361 aatggtgaat tatgtaaaat tgataaggct tattccgggg aaattgttat tttgcaaaat
     6421 gagtttttga agctaaatag tgttcttgga gatacaaagc tattgccaca gagagagaga
     6481 attgaaaatc cgctccctct gctgcaaaca actgttgaac cgagcaaacc tcaacaaagg
     6541 gaaatgttac ttgatgcact tttagaaatc tccgacagtg acccgcttct acaatattat
     6601 gtggattcta cgacacatga aattatactt tctttcttag ggaaagtaca aatggaagtg
     6661 attagtgcac tgttgcaaga aaagtatcat gtggagatag aactaaaaga gcctacagtc
     6721 atttatatgg agagaccgtt aaaaaatgca gaatatacca ttcacatcga agtgccgcca
     6781 aatcctttct gggcttccat tggtttatct gtatcaccgc ttccgttggg aagtggaatg
     6841 cagtatgaga gctcggtttc tcttggatac ttaaatcaat catttcaaaa tgcagttatg
     6901 gaagggatac gctatggttg cgaacaagga ttatatggtt ggaatgtgac ggattgtaaa
     6961 atctgtttta agtatggctt atactatagc cctgttagta ccccagcaga ttttcggatg
     7021 cttgctccta ttgtattgga acaagtctta aaaaaagctg gaacagaatt gttagagcca
     7081 tatcttagtt ttaaaattta tgcgccacag gaatatcttt cacgagcata caacgatgct
     7141 cctaaatatt gtgcgaacat cgtagacact caattgaaaa ataatgaggt cattcttagt
     7201 ggagaaatcc ctgctcggtg tattcaagaa tatcgtagtg atttaacttt ctttacaaat
     7261 ggacgtagtg tttgtttaac agagttaaaa gggtaccatg ttactaccgg tgaacctgtt
     7321 tgccagcccc gtcgtccaaa tagtcggata gataaagtac gatatatgtt caataaaata
     7381 acttagtgta ttttatgttg ttatataaat atggtttctt gttaaataag atgaaatatt
     7441 ttttaataaa gatttgaatt aaagtgtaaa ggaggaatta attatgaagt gtaatgaatg
     7501 taacagggtt aaatacaacc aaattttaat ttggctttgc gttttatctt tttttagcgt
     7561 attaaatgaa atggttttga acgtctcatt acctgatatt gcaaatgatt ttaataaacc
     7621 acctgcgagt acaaactggg tgaacacagc ctttatgtta accttttcca ttggaacagc
     7681 tgtatatgga aagctatctg atcaattagg catcaaaagg ttactcctat ttggaattat
     7741 aataaattgt ttcgggtcgg taattgggtt tgttggccat tctttctttt ccttacttat
     7801 tatggctcgt tttattcaag gggctggtgc agctgcattt ccagcactcg taatggttgt
     7861 agttgcgcgc tatattccaa aggaaaatag gggtaaagca tttggtctta ttggatcgat
     7921 agtagccatg ggagaaggag tcggtccagc gattggtgga atgatagccc attatattca
     7981 ttggtcctat cttctactca ttcctatgat aacaattatc actgttccgt ttcttatgaa
     8041 attattaaag aaagaagtaa ggataaaagg tcattttgat atcaaaggaa ttatactaat
     8101 gtctgtaggc attgtatttt ttatgttgtt tacaacatca tatagcattt cttttcttat
     8161 cgttagcgtg ctgtcattcc tgatatttgt aaaacatatc aggaaagtaa cagatccttt
     8221 tgttgatccc ggattaggga aaaatatacc ttttatgatt ggagttcttt gtgggggaat
     8281 tatatttgga acagtagcag ggtttgtctc tatggttcct tatatgatga aagatgttca
     8341 ccagctaagt actgccgaaa tcggaagtgt aattattttc cctggaacaa tgagtgtcat
     8401 tattttcggc tacattggtg ggatacttgt tgatagaaga ggtcctttat acgtgttaaa
     8461 catcggagtt acatttcttt ctgttagctt tttaactgct tcctttcttt tagaaacaac
     8521 atcatggttc atgacaatta taatcgtatt tgttttaggt gggctttcgt tcaccaaaac
     8581 agttatatca acaattgttt caagtagctt gaaacagcag gaagctggtg ctggaatgag
     8641 tttgcttaac tttaccagct ttttatcaga gggaacaggt attgcaattg taggtggttt
     8701 attatccata cccttacttg atcaaaggtt gttacctatg gaagttgatc agtcaactta
     8761 tctgtatagt aatttgttat tacttttttc aggaatcatt gtcattagtt ggctggttac
     8821 cttgaatgta tataaacatt ctcaaaggga tttctaaatc gttaagggat caactttggg
     8881 agagagttca aaattgatcc tttttttata acaggaattc aaatcttttt gttccattaa
     8941 agggcgcgat tgctgaataa aagatacgag agacctctct tgtatctttt ttattttgag
     9001 tggttttgtc cgttacacta gaaaaccgaa agacaataaa aattttattc ttgctgagtc
     9061 tggctttcgg taagctagac aaaacggaca aaataaaaat tggcaagggt ttaaaggtgg
     9121 agattttttg agtgatcttc tcaaaaaata ctacctgtcc cttgctgatt tttaaacgag
     9181 cacgagagca aaacccccct ttgctgaggt ggcagagggc aggttttttt gtttcttttt
     9241 tctcgtaaaa aaaagaaagg tcttaaaggt tttatggttt tggtcggcac tgccgacagc
     9301 ctcgcagagc acacacttta tgaatataaa gtatagtgtg ttatacttta cttggaagtg
     9361 gttgccggaa agagcgaaaa tgcctcacat ttgtgccacc taaaaaggag cgatttacat
     9421 atgagttatg cagtttgtag aatgcaaaaa gtgaaatcag ctggactaaa aggcatgcaa
     9481 tttcataatc aaagagagcg aaaaagtaga acgaatgatg atattgacca tgagcgaaca
     9541 cgtgaaaatt atgatttgaa aaatgataaa aatattgatt acaacgaacg tgtcaaagaa
     9601 attattgaat cacaaaaaac aggtacaaga aaaacgagga aagatgctgt tcttgtaaat
     9661 gagttgctag taacatctga ccgagatttt tttgagcaac tggatccagg agaacaaaaa
     9721 cgattttttg aggaaagtta taaattattt tccgaacgat atggcaagca aaatattgct
     9781 tatgcaacag ttcataatga tgagcaaacc cctcacatgc atttaggtgt tgtgcctatg
     9841 cgtgatggaa aactgcaagg aaaaaatgtg tttaatcgtc aagaactgtt atggctacaa
     9901 gataaattcc ccgagcatat gaaaaaacag ggttttgagt tgaagcgtgg tgaacgtggc
     9961 tctgaccgta aacatattga gacagctaaa tttaaaaaac aaactttgga aaaagagatt
    10021 gattttctag aaaaaaattt agcagttaaa aaagatgaat ggactgctta tagcgataaa
    10081 gttaaatcag atttagaagt accagcgaaa cgacacatga aaagtgttga agtgccaacg
    10141 ggtgaaaagt ccatgtttgg tttgggaaaa gaaataatga aaacagaaaa gaaaccaacc
    10201 aaaaatgttg ttatatcgga gcgtgattat aaaaacttag tgactgctgc gagagataac
    10261 gataggttaa aacagcatgt tagaaatctc atgagtactg atatggcgag agaatataaa
    10321 aaattaagta aagaacatgg gcaagttaaa gaaaaatata gtggtcttgt agagcgattt
    10381 aatgaaaatg taaatgatta taatgagttg cttgaagaaa acaagtcttt aaagtctaaa
    10441 ataagcgatt taaagcgtga tgtgagttta atctatgaaa gcactaagga attccttaag
    10501 gaacgtacag acggcttaaa agcctttaaa aacgttttta aggggtttgt agacaaggta
    10561 aaggataaaa cagcacaatt ccaagaaaaa cacgatttag aacctaaaaa gaacgaattt
    10621 gaactaactc ataaccgaga ggtaaaaaaa gaacgaagtc gagatcaggg aatgagttta
    10681 taaaataaaa aaagcacctg aaaaggtgtc tttttttgat ggttttgaac ttgttctttc
    10741 ttatcttgat atatatagaa ataacgtcat ttttatttta gttgctgaaa ggtgcgttga
    10801 agtgttggta tgtatgtgtt ttaaagtatt gaaaaccctt aaaattggtt gcacagaaaa
    10861 accccatctg ttaaagttat aagtgaccaa acaaataact aaatagatgg gggtttcttt
    10921 taatattatg tgtcctaata gtagcattta ttcagatgaa aaatcaaggg ttttagtgga
    10981 caagacaaaa agtggaaaag tgagaccatg gagagaaaag aaaatcgcta atgttgatta
    11041 ctttgaactt ctgcatattc ttgaatttaa aaaggctgaa agagtaaaag attgtgctga
    11101 aatattagag tataaacaaa atcgtgaaac aggcgaaaga aagttgtatc gagtgtggtt
    11161 ttgtaaatcc aggctttgtc caatgtgcaa ctggaggaga gcaatgaaac atggcattca
    11221 gtcacaaaag gttgttgctg aagttattaa acaaaagcca acagttcgtt ggttgtttct
    11281 cacattaaca gttaaaaatg tttatgatgg cgaagaatta aataagagtt tgtcagatat
    11341 ggctcaagga tttcgccgaa tgatgcaata taaaaaaatt aataaaaatg gttctgttgc
    11401 aaagttttaa ataaagaata aaatccc
//
LOCUS       DAFBQH010000208         7959 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.guided.100, whole genome shotgun sequence.
ACCESSION   DAFBQH010000208 DAFBQH010000000
VERSION     DAFBQH010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.guided.100"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            128..1564
                     /locus_tag="LK694_002932"
     CDS             128..1564
                     /locus_tag="LK694_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK5053695.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="LK694_002933"
     CDS             1753..4200
                     /locus_tag="LK694_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBK5053696.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="LK694_002934"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="LK694_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBK5053697.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="LK694_002935"
     CDS             complement(5690..6793)
                     /locus_tag="LK694_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBK5053698.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="LK694_002936"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="LK694_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBK5053699.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2665 a         1359 c         1729 g         2206 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DAFBQH010000209         7779 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.guided.103, whole genome shotgun sequence.
ACCESSION   DAFBQH010000209 DAFBQH010000000
VERSION     DAFBQH010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.guided.103"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            245..1213
                     /locus_tag="LK694_002937"
     CDS             245..1213
                     /locus_tag="LK694_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBK5053700.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="LK694_002938"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="LK694_002938"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBK5053701.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="LK694_002939"
     CDS             2243..2851
                     /locus_tag="LK694_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBK5053702.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3257..4189
                     /gene="vanY"
                     /locus_tag="LK694_002940"
     CDS             3257..4189
                     /gene="vanY"
                     /locus_tag="LK694_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HBK5053703.1"
                     /translation="MVKEETENEEVVFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYD
                     QNPKEHLENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDEL
                     INGYGLLDSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAE
                     YALPAGYSEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQ
                     YEPWHIRYVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTT
                     IHVPTNLRYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4342..4827
                     /gene="vanZ"
                     /locus_tag="LK694_002941"
     CDS             4342..4827
                     /gene="vanZ"
                     /locus_tag="LK694_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBK5053704.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5114..5443)
                     /locus_tag="LK694_002942"
     CDS             complement(5114..5443)
                     /locus_tag="LK694_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK5053705.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            5748..6077
                     /locus_tag="LK694_002943"
     CDS             5748..6077
                     /locus_tag="LK694_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK5053706.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            6067..6354
                     /locus_tag="LK694_002944"
     CDS             6067..6354
                     /locus_tag="LK694_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK5053707.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(6633..7196)
                     /locus_tag="LK694_002945"
     CDS             complement(6633..7196)
                     /locus_tag="LK694_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5053708.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         2566 a         1298 c         1546 g         2369 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga tgtaaggcag agcgatattg cggtcattat ctgcgtgcgc
     3001 tgcggcaaga tagcctgata ataagactga tcgcatagag gggtggtatt tcacaccgcc
     3061 cattgtcaac aggcagttca gcctcgttaa attcagcatg ggtatcactt atgaaaattc
     3121 atctacattg gtgataatag taaatccagt agggcgaaat aattgactgt aatttacggg
     3181 gcaaaacggc acaatctcaa acgagattgt gccgtttaag gggaagattc tagaaatatt
     3241 tcatacttcc aactatatag ttaaggagga gactgaaaat gaagaagttg tttttttatt
     3301 gttattgtta ttcttaatat acttaggtta tgactacgtt aatgaagcac tgttttctca
     3361 ggaaaaagtc gaatttcaaa attatgatca aaatcccaaa gaacatttag aaaatagtgg
     3421 gacttctgaa aatacccaag agaaaacaat tacagaagaa caggtttatc aaggaaatct
     3481 gctattaatc aatagtaaat atcctgttcg ccaagaaagt gtgaagtcag atatcgtgaa
     3541 tttatctaaa catgacgaat taataaatgg atacgggttg cttgatagta atatttatat
     3601 gtcaaaagaa atagcacaaa aattttcaga gatggtcaat gatgctgtaa agggtggcgt
     3661 tagtcatttt attattaata gtggctatcg agactttgat gagcaaagtg tgctttacca
     3721 agaaatgggg gctgagtatg ccttaccagc aggttatagt gagcataatt caggtttatc
     3781 actagatgta ggatcaagct tgacgaaaat ggaacgagcc cctgaaggaa agtggataga
     3841 agaaaatgct tggaaatacg ggttcatttt acgttatcca gaggacaaaa cagagttaac
     3901 aggaattcaa tatgaaccat ggcatattcg ctatgttggt ttaccacata gtgcgattat
     3961 gaaagaaaag aatttcgttc tcgaggaata tatggattac ctaaaagaag aaaaaaccat
     4021 ttctgttagt gtaaatgggg aaaaatatga gatcttttat tatcctgtta ctaaaaatac
     4081 caccattcat gtgccgacta atcttcgtta tgagatatca ggaaacaata tagacggtgt
     4141 aattgtgaca gtgtttcccg gatcaacaca tactaattca aggaggtaag gatggcggaa
     4201 tgaaaccaac gaaattaatg aacagcatta ttgtactagc acttttgggg taacgttagc
     4261 tttttaattt aaaacccacg ttaactagga cattgctata ctaatgatac aacttaaaca
     4321 aaagaattag aggaaattat attgggaaaa atattatcta gaggattgct agctttatat
     4381 ttagtgacac taatctggtt agtgttattc aaattacaat acaatatttt atcagtattt
     4441 aattatcatc aaagaagtct taacttgact ccatttactg ctactgggaa tttcagagag
     4501 atgatagata atgttataat ctttattcca tttggcttgc ttttgaatgt caattttaaa
     4561 gaaatcggat ttttacctaa gtttgctttt gtactggttt taagtcttac ttttgaaata
     4621 attcaattta tcttcgctat tggagcgaca gacataacag atgtaattac aaatactgtt
     4681 ggaggctttc ttggactgaa attatatggt ttaagcaata agcatatgaa tcaaaaaaaa
     4741 ttagacagag ttattatttt tgtaggtata cttttgctcg tattattgct cgtttaccgt
     4801 acccatttaa gaataaatta cgtgtaagat gtctaaatca agcaatctga tctttcatac
     4861 acataaagat attgaatgaa ttggattaga tggaaaacgg gatgtgggga aactcgcccg
     4921 taggtgtgaa gtgaggggaa aaccggtgat aaagtaaaaa gcttacctaa cactatagta
     4981 acaaagaaag cccaattatc aattttagtg ctgaggaatt ggtctcttta ataaatttcc
     5041 ttaacgttgt aaatccgcat tttcctgacg gtacccccaa actaagattt catagataga
     5101 ttttctgata actttaaaaa acacgaatag cggaaaggat gtcttcattc gctaaagagt
     5161 aataaactac ttttccatct tgacgtgatt ttgccattcc atgttttttt aaaaaacgta
     5221 aatggtgtga cgttgtagcg acactagcag taagtatttc tgctaaatca cagacgcaca
     5281 tttccttata ggtttctaaa gcatagaaaa ttttaattct agaaaaatcg ctgaaacatt
     5341 tccctaacac aagtaagttt gaaaaatctt tttgatccaa tttatttttt attccattta
     5401 ctttttcttt atgtacgaca gtcacttggc aaactttttt cattcatctc acctcacatt
     5461 caaacgatta tttgaatgaa gtatacctat ttgatcactc ttttgtcaat ctaccttaat
     5521 tccaatacac tattataagc aatttcatgt attgaagtaa acttacttgc aagtgcgttt
     5581 ttgattccaa gttaaaaaca tattattatt ctacataatt tcctaatcaa ataaaactcc
     5641 tttgaaaatt gttctaaagg agttttatta ttttgtattt ctactaaaaa tagcgtacaa
     5701 tgtaattact aagtaattac gaaatgtaaa ataatggagg aaataatatg attacaaaaa
     5761 caagaaaaca aggaaattct attatgttaa cggtccctaa agaattcgat gtgcccaatg
     5821 gtgttgaagt agaagctaag cttgtagaga atggtatcct ttatgagttt gtagagccta
     5881 aaaaagaatt ttttgatttt agtgaagata ttttggctga tattctttca gaaggttata
     5941 gcaaacaaga gatcttaaaa gaatttagaa atagaaaaaa tgcgttaact tctgcattta
     6001 aatctattgc tgaagatact gttgcaaatt ctaaaccgat gacaaaagag gagttggcag
     6061 cagaaattgg cttatgagat tctaccttct aaacacgtaa taaagtatct aaaaaaatta
     6121 aaagagaaaa cattaaaaga acaattttta acgataattt atgatgagat agcagttcgt
     6181 cctcattcgg gagaacaaaa aacgggtgat ttatcaggaa tttgggctat tggattcaag
     6241 tatgcaggta cgacatatcg cgttgcatat gaaattaaag ataatgcagt gatacctatc
     6301 ttactatgtg gaacgcatga aaatttttat gaacaattaa aaaagattag gtaagaaccc
     6361 aatgtttcaa cttgagagga tagacgacac tttaaaagat aatttttaac agctgagagt
     6421 tgtaataccc tataatttta aaccactaac ctatatattt atgaaaaata gaaaaaatta
     6481 aaacgcattt taagcctctt tcaaattaaa agcttcatcc ttatgcacaa agagggctta
     6541 aaatgcgtta ttttatattg ttttttagta ataataaaaa aatcactcta attctcccat
     6601 aaatattata gattcctaaa gattaaaata gattattccc tattgtcttt taagtagcgg
     6661 tagagtgttg ccttgctaat accagtcatt tccgtaattt cttttacgct ataaacctga
     6721 cttttataca attttactgc tcgatcaaca tctttctggt tcactcgtgg acggccacct
     6781 ttacgacctc tcgcacgtgc acttttcaaa ccttctttgg tacgttcaac aattaaatca
     6841 cgttcgaact gactaaatgc ttggaagaca gtcatcatta agcgtccgtg aggcgtgctg
     6901 gtatcgaagt tttctttcaa gctcaccagc ttaacatggt gttcttcaaa ataggtaaca
     6961 aggtcaatca aatcctttgt actacgaccc aatcgagaaa aactttctac aattaccgta
     7021 tctcccggac gaattttatc ttttaaacga tttagttggg gacgaaccgc tttggttccc
     7081 gtcattttct cagttaggat ttcattacaa ttttgttcat tgagtaagtc taattgacga
     7141 tttagttctt gtgaacgtgt actcactcgg gcataaccat aaatgtatcc actcatgctt
     7201 ttcttccttt cgtactttaa ttctggcttg attgtatcat aaaagggagt tatagataca
     7261 tagttaatgc aacaagttat gatacaaaat atgacctctt ttatttaata aaagaggtca
     7321 tttcattttg tctcataatc gattgttttt gatacgagtg tgtatacgaa ataaacaaaa
     7381 aaatcttaag taaatttcta gatacctaat ttaatcctaa ctagtgtatc ctaaagttaa
     7441 taacgagttt atcacaatga aaattataaa atgctcccat attagatata acatgcaata
     7501 tttgaattag aactacaaaa tttattcgtt ttataaagta aataatgttg agatactaag
     7561 gaatagaata ttataaggaa ttgagctgaa tgtattggat aaaaaaagaa taactttatt
     7621 agagatggac tataaaaatg ctcgtaattt tcttctgaag tctgaaagct atttgaatgc
     7681 tcagctacca gaatatatta gttttgacga aactttaaaa tctgctaaaa aagttttaac
     7741 ttcgccgttt gtataattaa gcgagacaaa taaaaaagc
//
LOCUS       DAFBQH010000210         6459 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.guided.110, whole genome shotgun sequence.
ACCESSION   DAFBQH010000210 DAFBQH010000000
VERSION     DAFBQH010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.guided.110"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            305..871
                     /gene="amaP"
                     /locus_tag="LK694_002946"
     CDS             305..871
                     /gene="amaP"
                     /locus_tag="LK694_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBK5053709.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            884..1072
                     /locus_tag="LK694_002947"
     CDS             884..1072
                     /locus_tag="LK694_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBK5053710.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1085..1642
                     /locus_tag="LK694_002948"
     CDS             1085..1642
                     /locus_tag="LK694_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBK5053711.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1674..1916
                     /locus_tag="LK694_002949"
     CDS             1674..1916
                     /locus_tag="LK694_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK5053712.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            2257..3471
                     /locus_tag="LK694_002950"
     CDS             2257..3471
                     /locus_tag="LK694_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBK5053713.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            3502..3720
                     /locus_tag="LK694_002951"
                     /pseudo
     CDS             3502..3720
                     /locus_tag="LK694_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4139..4612
                     /gene="dfrF"
                     /locus_tag="LK694_002952"
     CDS             4139..4612
                     /gene="dfrF"
                     /locus_tag="LK694_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBK5053714.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            4739..5395
                     /locus_tag="LK694_002953"
     CDS             4739..5395
                     /locus_tag="LK694_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBK5053715.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            5577..6107
                     /locus_tag="LK694_002954"
     CDS             5577..6107
                     /locus_tag="LK694_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBK5053716.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
BASE COUNT         2222 a          963 c         1356 g         1918 t
ORIGIN      
        1 taaaatgtgc caaagaatta aagctatgaa ttattcagga gaatcttgta ataataactg
       61 agcataatct ttgcaatttt taatcttgaa taatatcctt taagtatagc cagcaatcaa
      121 aagtaaatag agaataagta agcaattgtt atttcagtat cagactggca ataaaacgcc
      181 gttatgatcc agcaatgttt ggtagcgctt tatttactga tgctatgcta actgttcgct
      241 atctatactg tgattgtcgg tagagttatt tacagggtaa aaataagttt ataggaggtg
      301 gaaaatgaat aaggggttaa aaataatcgg tgttatcatg agtttactgc tactttcagt
      361 atttctagcg attgcgttga tcaatgcgcc gtatattatg cctgaacaat tggaacgatt
      421 tcgcttcttt actttaacga attactacat gcaacaatat attttctggg cagctgtagt
      481 atttgccgtt cttgctatta tcatcttgct agtcgttctt ttctatccaa agtcaagagg
      541 aactttcgtt atgaaacggg aagatggcaa actaacaatc gacaaaaaag cgatcgaagg
      601 gttggtacgt tcccatttac acgaagaaga gttcattcat tcacctaaag tacggatacg
      661 ttcaacgaaa aatcgaatcc atatccatgt caacggagac ttgaaacgaa cttcttcatt
      721 agtagggaaa acaggtgtat tgatgcaaga tatcgaagaa gaagtaacta aagtactcgg
      781 aacaaaagaa acggtcaaag tggcagtcac atacagcggt tatgaagaac aagaagatac
      841 aagagactac aagcactctc gagtggaata ggagggccaa ataatgaaag aactattttc
      901 agcatacaag ctccctatta tctttggggg tcttggacta ctgctagctg ttttatggtt
      961 aacgattgga tttttcaaga cattgttgat cataatcatg acagcaattg gcgcaggaat
     1021 cggtttttat ttgaaacagt caggaatagt tgatcaattt tttaaatcat aggaggaaac
     1081 aaagatggaa aacaaaaaca tgaatacaac aaatggatca gttttggcaa atgaaaaagg
     1141 agaaaaccgc atgatgaatg gtgaattgac ttatgaagac aaagtcatcc aaaaaatcat
     1201 cggttatgca ttgaaagatg tagatggtct attgacatta gatggcggat ttttctcaaa
     1261 tatggctgaa aaattagtca acacagataa cgtaacagca ggtatcaata cagaagtagg
     1321 taagaaacaa gtagctgtgg atctagatgt gatcgtagaa tacggtaaag acatcgaaga
     1381 tatttacaac caaatcaaag aattgatcag tactgaagta aacaatatga cacatttaga
     1441 tgttatcgaa gtgaatgtga atgttgcaga catcaaaaca caagaagaat accaaaaaga
     1501 tcaagaaaca gtacaagaca aagtaacaga agcagcaaaa tccacaggac aatttgcttc
     1561 acgccaaaca gataaagcaa aatctgctgt aggcaaaggt gcacaaaaag tcaaagaaaa
     1621 taacgaacca cgtgtacaat aagaagtaag aagtcagtta ggaggaattt aaaatgggat
     1681 ttatttgggc attaatcgtc ggcggtatca ttggagcaat tgcaggagca atcacgaata
     1741 aaggatcatc aatggggatc atcgcaaaca tcattgctgg tttagtaggt tcagcaattg
     1801 gacaagcttt actaggaact tggggaccaa caattgcagg aatggctatc attccatcat
     1861 tgatcggtgc aatcatttta gtattagtcg tttcattctt tgcacgtaaa ttataagcaa
     1921 attgaagaaa aacttatgtt gaaatgaaga acaaatttga gaaccgtagc gacactcgct
     1981 gcggttcttt tttatatatt ttatatgaag tattctttat tccttatgaa aaaatatctt
     2041 taaaaataaa aaaagacgaa aataaacggt tttctataaa aagaagaccg tttatttttt
     2101 gtctttattt gtgtgaaaaa cgaaaaagag cacaacacat caagctttta ttaattgtct
     2161 gtcttttata aatgcaagta ttaattcaaa aaaacagaag aaaaaaacaa ctacaccttc
     2221 ttttatactc gtaatcgtag aaaggaggcg aaaaaaatga atacattacc tatcaataca
     2281 ggagatacag cattcatgat tttatgtaca gcaatggttt gcctgatgac tcctggtcta
     2341 gctttttttt acggcggatt agcacggaag aataatatac taacgatcat gtctcaatct
     2401 tttctctccg ttgggataac aacgattatg tgggtatttg gtggattcgg attggccttt
     2461 ggacgggata tcggcggtgt tattggaaac ccggcagatt tctttttgat gagacatgtt
     2521 acaagcttcc ccaacatgtt tcatggtgca acgattccat ttttaatgtt ctttctttac
     2581 caactgatgt ttatcgtgat taccgttcct ttgatgacag gagcttttgc agggagattg
     2641 aatttaaaag gctatattat acttgttatt ttttggaatc tgctgattta tttccctgtt
     2701 tgccactgga tatggggtgg cggatttttg gatcagatgg gatttagaga tttcgctggt
     2761 ggagcagtca tccacacgac agctggattc ggctctttag catgtatcct cacacttggt
     2821 caaagaaaga ttcctttagg agaaagaagt cagcatagca gtcttatggc agctgctatc
     2881 ggtacaggac tgctttggtt tggctggttc ggttttaatt ccggtggtgc acttcgagca
     2941 gatgaacaag cagtcaatgc atttggttca acattcatcg ctcttgcatt tgcgatgatt
     3001 acttggctga tcattgcaaa agtaaaagga aatggctttg attttgtcga cgtactaaca
     3061 ggatccgttg ctggattggc agccattacg ccttgtgccg gttatgtaga agcaaaaagt
     3121 gccatgctga ttgggatcat tgcgggtatc gtctgtcatg ctgctgttga ttttcgtaaa
     3181 aagaaacaat gggatgatgc acttgatgtg tggggtgttc atggcatggg cggattcaca
     3241 ggtacgattc tgatagggat ctttgcatct aacagccatt tactaacgaa tccaagcagc
     3301 tggtacttct tagggattca agtgattggt gtcatcatta ctgccgtgta tgcctatgtg
     3361 ctgacaacag tgattttaaa atcggcgatg cactttacaa cgatcactac tacaaaagaa
     3421 gaacaagaag aaggattgga ctttttcttc tttggcaaca aactcaattg aaacaaattc
     3481 aaatagaaaa gaggaagatt catgcaacaa agcaaaacga aaattcctgc aaaaaaatta
     3541 acgttctttg gctttttggc tatgaccatc tcaatggtcg tttcattata tgaatatccc
     3601 acattcgcga cctcaggctt cagcttagta tttttcttgt tattaggcgg tttgctatgg
     3661 tttatccctg tggcattgtg cgcagcagag atggcaacgg tgaaaggatg ggaaaaaggt
     3721 gtttcccaaa caaaaagata ttaagaaaca gcccatgttt acctgcttgc aaacaaaaga
     3781 atattgctac tagactaaga gatactacat aagtgtgttg gtatctcttt tttgttgccg
     3841 tgaaacagca gactatttca tttctgcttg attaccttaa aacttattag tctatggttt
     3901 gtcaagggct tgttgcgtga gaaatgctga atatcaccct ttacaaaaca taggctgata
     3961 agtaactttt caaaagcaga aaagaaatgg caatgcttat aacaatatga tataatgcag
     4021 atgaagacaa aaaaggaatt tgtcgagctg gttagttcga gataattttg agtttgttaa
     4081 gatgtggtgc acttgatgga atcacaggta cgacatcata ttatggagga tgaaatagat
     4141 gataggtttg attgttgcga ggtcaaagaa taatgttata ggcaagaatg gtaatatacc
     4201 atggaaaata aagggagaac aaaagcaatt tagagagtta acaacgggta atgtggttat
     4261 tatggggcga aagtcttatg aagaaatcgg tcatccgttg cctaatagaa tgaatattgt
     4321 tgtttccacc acaacagagt atcaaggaga taatttagtt tcagttaaat cattagaaga
     4381 tgcattatta ttggctaaag gacgagatgt atacatatct ggtggatatg gactatttaa
     4441 ggaagctttg caaatagtag ataaaatgta tatcacagaa gtagatttaa atattgaaga
     4501 tggagataca ttctttccag aatttgatat caatgatttt gaagttttga taggggaaac
     4561 acttggtgag gaagtgaaat atacgagaac attttatgta aggaaaaaat gaattgagta
     4621 aattttggat ttagggtgtt ttcataaata tattgctttt tatgcatata taaattgttg
     4681 taagacaaat tccagtttgt atggggcgat aaaatcagaa tttgagagag gattgaatat
     4741 gaataaggaa tggtctgaac ttaataaaac aatgcaggca caaataaaaa agaaagatac
     4801 ttataagagg ggtattgata ctttacttac tttacgaagt cagttaatac aaaccttagt
     4861 atctttcaaa gaagaattat gcagagaaga ttttaactca atccccttca taaacgctga
     4921 tggttatcat agtaagacga ttgcttattc tatttggcat attttccgca ttgaagatat
     4981 agttgtgcat acagtgataa atgaagatga acaagtattt tttgcaggca attatcaaga
     5041 gcgtatcaat tcacctatca ttacaacagg aaatgaactc atgaaacagc agattgcaga
     5101 cttttcaaaa cagcttaatc tggaagaatt atatttatat attttcgaag tatgggaaag
     5161 cactgagaaa atgcttgaac ggttatctta tgatgaattg aaaagaaaaa taccgaaaga
     5221 aagaaaagga tacttagaat cgttgaatgt agtaaacgac aatgaaaaag caatttggct
     5281 gattgattat tggtgtaata aagatatttg tggactaatt caaatgccgt tttcaagaca
     5341 ttggattatg cacacagaag cctgtttgcg tataaagaac aagatacatt cataggcaaa
     5401 aatttcagtt tgtagaacta aggaaattgg aatttaagga ggaataaagt atggttagtg
     5461 attttataag agcagcattt ccatggattt taatgggttt atttgtagca atcagttgtt
     5521 cccttatgag taaaaaagag aaataaattc ctgtttgtat ttataaaaga gaaaaaatga
     5581 gaaaagtagt tttatttatt gccatgagcc ttgacggata tattgcggac ggtaatggtg
     5641 gagtagcttg gctaaatggt catggaaatg acaatgaaaa catcgacact tatacagaat
     5701 ttaccaaaga tatagatact gtcttaatgg ggtggaatac ttctcatcag gttgttactg
     5761 aactttctcc gcaagaatgg gtatataata aatttacaac ttatgtatta acacataaag
     5821 agtgcaattc ttcccaacaa attcatttta caagtgaaaa tcctgtttta ttattggaac
     5881 ggctaaaaca agaagcagga aaggatattt ggatttgtgg cggagcaaat cttgttcagc
     5941 agttggtaag caaaaatatg attgataaat attacatttc tgttattcct actctgttag
     6001 gaaatggggt tcgtcttttg ggcaacatag acagggaaat aaagttaagg ctttgtaaaa
     6061 cacagactta taacggaata accgatttga tttatatgcg tagataacta gagagaaaca
     6121 gaaaagctgt taaaccgacg gagcgatccg accacgttgg cgggaaaatc cttaagattt
     6181 ttccgccatg cttgcaaggg ggcggttcgc ttcggcggtt cggcgagctt gcgagccgtt
     6241 caaagccccc gttatctaac aaggggggca caaaaaacaa gaaacactat acactaacaa
     6301 gcggaaaacg ctgtatttat aaggcaaaac cccattttta cagtagtgat ataaattgta
     6361 cctatatcac tatgaaaatt gaatagtaaa tatgagaaag acagttgatt tcaaaaaata
     6421 ggctctttgt caataaggac tgatgggttg tacaaaatc
//
LOCUS       DAFBQH010000211         4995 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.guided.122, whole genome shotgun sequence.
ACCESSION   DAFBQH010000211 DAFBQH010000000
VERSION     DAFBQH010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.guided.122"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            complement(30..2180)
                     /locus_tag="LK694_002955"
                     /pseudo
     CDS             complement(30..2180)
                     /locus_tag="LK694_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2326..2901
                     /locus_tag="LK694_002956"
     CDS             2326..2901
                     /locus_tag="LK694_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK5053717.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3115..3810
                     /gene="vanR"
                     /locus_tag="LK694_002957"
     CDS             3115..3810
                     /gene="vanR"
                     /locus_tag="LK694_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBK5053718.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3788..4942
                     /locus_tag="LK694_002958"
     CDS             3788..4942
                     /locus_tag="LK694_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBK5053719.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1560 a          939 c          967 g         1529 t
ORIGIN      
        1 aattttcttc tttcagcttt ttatttccca cttgtaaatc ggtagatggt ggctcctttt
       61 ccagttccat aatgtggatt tgcatctgca tgtagtgatg aaacacctag ctgcattctc
      121 ataccatctg acgaagatgt tgtaccgtcg ccccaataga aaggcaattg taatttatga
      181 tgaaagttta ctaatatggc ttgggcttta ttcatggcat cttcatacat gcgccattga
      241 gatacattgg ctagttgctt atatgtaagt ccgggtgtgg cttcggccat cttgctcaag
      301 ccaatattca ttcccattcc taaaagggca gccatgataa tgattgtttc ttccttatct
      361 ggttttcgat tattggaagc atgagtgaat tgctcatgaa atcctgttat atgggccaca
      421 tccatgagta aatcagttaa ttttattctt ggtagcatct gataaaggct tgcactaaat
      481 ttttttgctt cttctggaac atctttttct aagcgtgcaa gtgatagctt tcctttttca
      541 agagaaaccc catctaactt attggaattg gcagctaacc actttaacct ttcattaaag
      601 ctgctggttc tctccgttat ataatcttcg aatgataaac taactgataa tctcgtattc
      661 cccttcgatt gattccatgt atcttccgaa aacaaatatt cctcaaaatc cctatattgt
      721 ctgctgccaa caatggaaac atctcctgcc cgaacatgct cccgaagttc tgttaaaaca
      781 gccatttcat agtaatgacg attaattgtt gtaccatcat cctcgtataa atgtcttttc
      841 catcgttttg aaataaaatc cacaggtgag tcatcaggca cttttcgctt tccagattcg
      901 ttcattcctc ggataatctc aacagcttgt aaaagtggct catttgcctt tgtagaatga
      961 aattccaata ctcttaatag cgttggcgta tattttctta gtgaataaaa ccgtttttgc
     1021 agtaagtcta aataatcata gtcggcagga cgtgcaagtt cctgagcctc ttctactgaa
     1081 gagacaaagg tattccattc aataaccgat tctaaaacct taaaaacgtc taatttttcc
     1141 tctcttgctt taattaatgc ttgtccgatg ttcgtaaagt gtataacttt ctcatttagc
     1201 tttttaccgt tttgtttctg gatttcctct tgagccttac gaccttttga taacaaacta
     1261 agtatttgcc tatcatgaat ttcaaacgct ttatccgtta gctcctgagt aagttgtaat
     1321 aaatagatgg ttaatatcga ataacgttta ttttcttgaa agtcacggaa tgcatacggc
     1381 tcgtatcttg agcctaagcg agacagctgc aacaggcggt tacggtgcaa atgactaatt
     1441 tgcactgttt ctaaatccat tcctcgtatg tattcgagtc gttctattat ttttagaaaa
     1501 gtttcgggtg aaggatgacc cggtggctct tttaaccaac ccaatatcgt tttattggat
     1561 tcggatggat gctgcgaggt aataatccct tcaagctttt ctttttgctc atttgttaga
     1621 gatttactaa ccgtattaaa tagcttcttt tcagccattg cccttgcttc ccacaccatt
     1681 ctttcaagtg tagtgatagc aggcagtata attttgtttt ttcttagaaa atctatgcat
     1741 tcatgcagta gatgaatggc atcaccattt tccaaagcta attgatgaag gtacttaaat
     1801 gtcattcgat attcactcag ggtaaaagtt acaaagtcgt attcacttcg aatttctttc
     1861 aaatgatccc aaagtgtatt ttccctttga ggataatgat caagcgagga tggactaaca
     1921 ccaatctgtt tcgatatata ttgtatgacc gaatctggga tgcttttgat atgagtgtat
     1981 ggccaaccgg gataccgaag aacagctaat tgaacagcaa atcctaaacg gttttcttcc
     2041 ctccttcgct tattaactat ttctaaatcc cgtttggaaa aagtgaagta ggtccccagt
     2101 atccattcat cttcagggat ttgcataaaa gcctgtctct gttccggtgt aagcaattct
     2161 ctacctctcg caattttcat tcagtatcat tccatttctg tattttcaat ttattagttc
     2221 aattatatat caatagagtg tactctattg atacaaatgt agtagactga taaaatcata
     2281 gttaagagcg tctcataaga cttgtctcaa aaatgaggtg atattttgcg gaaaatcggt
     2341 tatattcgtg tcagttcgac taaccagaat ccttcaagac aatttcagca gttgaacgag
     2401 atcggaatgg atattatata tgaagagaaa gtttcaggag caacaaagga tcgcgagcaa
     2461 cttcaaaaag tgttagacga tttacaggaa gatgacatca tttatgttac agacttaact
     2521 cgaatcactc gtagtacaca agatctattt gaattaatcg ataacatacg agataaaaag
     2581 gcaagtttaa aatcactaaa agatacatgg cttgatttat cagaagataa tccatacagc
     2641 caattcttaa ttactgtaat ggctggtgtt aaccaattag agcgagatct tattcggatg
     2701 agacaacgtg aagggattga attggctaag aaagaaggaa agtttaaagg tcgattaaag
     2761 aagtatcata aaaatcacgc aggaatgaat tatgcggtaa agctatataa agaaggaaat
     2821 atgactgtaa atcaaatttg tgaaattact aatgtatcta gggcttcatt atacaggaaa
     2881 ttatcagaag tgaataatta gccattctgt attccgctaa tgggcaatat ttttaaagaa
     2941 gaaaaggaaa ctataaaata ttaacagcct cctagcgatg ccgaaaagcc ctttgataaa
     3001 aaaagaatca tcatcttaag aaattcttag tcatttatta tgtaaatgct tataaattcg
     3061 gccctataat ctgataaatt attaagggca aacttatgtg aaagggtgat aactatgagc
     3121 gataaaatac ttattgtgga tgatgaacat gaaattgccg atttggttga attatactta
     3181 aaaaacgaga attatacggt tttcaaatac tataccgcca aagaagcatt ggaatgtata
     3241 gacaagtctg agattgacct tgccatattg gacatcatgc ttcccggcac aagcggcctt
     3301 actatctgtc aaaaaataag ggacaagcac acctatccga ttatcatgct gaccgggaaa
     3361 gatacagagg tagataaaat tacagggtta acaatcggcg cggatgatta tataacgaag
     3421 ccctttcgcc cactggagtt aattgctcgg gtaaaggccc agttgcgccg atacaaaaaa
     3481 ttcagtggag taaaggagca gaacgaaaat gttatcgtcc actccggcct tgtcattaat
     3541 gttaacaccc atgagtgtta tctgaacgag aagcagttat cccttactcc caccgagttt
     3601 tcaatactgc gaatcctctg tgaaaacaag gggaatgtgg ttagctccga gctgctattt
     3661 catgagatat ggggcgacga atatttcagc aagagcaaca acaccatcac cgtgcatatc
     3721 cggcatttgc gcgaaaaaat gaacgacacc attgataatc cgaaatatat aaaaacggta
     3781 tggggggttg gttataaaat tgaaaaataa aaaaaacgac tattccaaac tagaacgaaa
     3841 actttacatg tatatcgttg caattgttgt ggtagcaatt gtattcgtgt tgtatattcg
     3901 ttcaatgatc cgagggaaac ttggggattg gatcttaagt attttggaaa acaaatatga
     3961 cttaaatcac ctggacgcga tgaaattata tcaatattcc atacggaaca atatagatat
     4021 ctttatttat gtggcgattg tcattagtat tcttattcta tgtcgcgtca tgctttcaaa
     4081 attcgcaaaa tactttgacg agataaatac cggcattgat gtacttattc agaacgaaga
     4141 taaacaaatt gagctttctg cggaaatgga tgttatggaa caaaagctca acacattaaa
     4201 acggactctg gaaaagcgag agcaggatgc aaagctggcc gaacaaagaa aaaatgacgt
     4261 tgttatgtac ttggcgcacg atattaaaac gccccttaca tccattatcg gttatttgag
     4321 cctgcttgac gaggctccag acatgccggt agatcaaaag gcaaagtatg tgcatatcac
     4381 gttggacaaa gcgtatcgac tcgaacagct aatcgacgag ttttttgaga ttacacggta
     4441 taacctacaa acgataacgc taacaaaaac gcacatagac ctatactata tgctggtgca
     4501 gatgaccgat gaattttatc ctcagctttc cgcacatgga aaacaggcgg ttattcacgc
     4561 ccccgaggat ctgaccgtgt ccggcgaccc tgataaactc gcgagagtct ttaacaacat
     4621 tttgaaaaac gccgctgcat acagtgagga taacagcatc attgacatta ccgcgggcct
     4681 ctccggggat gtggtgtcaa tcgaattcaa gaacactgga agcatcccaa aagataagct
     4741 agctgccata tttgaaaagt tctataggct ggacaatgct cgttcttccg atacgggtgg
     4801 cgcgggactt ggattggcga ttgcaaaaga aattattgtt cagcatggag ggcagattta
     4861 cgcggaaagc aatgataact atacgacgtt tagggtagag cttccagcga tgccagactt
     4921 ggttgataaa aggaggtcct aagagatgta tataattttg gctctttgtc aaatgatgtt
     4981 ggtaaagaga aatct
//
LOCUS       DAFBQH010000212          866 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.guided.195, whole genome shotgun sequence.
ACCESSION   DAFBQH010000212 DAFBQH010000000
VERSION     DAFBQH010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.guided.195"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            <1..748
                     /gene="aph(2'')-I"
                     /locus_tag="LK694_002959"
     CDS             <1..748
                     /gene="aph(2'')-I"
                     /locus_tag="LK694_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HBK5053720.1"
                     /translation="TLYSILHRSLDLTNFLNTNLETNVKIPNIEYSYISDELSILGYK
                     EIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYI
                     LLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGI
                     IDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYP
                     IETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          368 a           66 c          143 g          289 t
ORIGIN      
        1 aacattgtat agcattttac acaggagtct ggacttgact aattttttaa atacaaattt
       61 agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg aattatctat
      121 actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt ctactatgtc
      181 agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac aaatgcacgg
      241 tttagattat acagatatta gtgaatgtac tattgataat aaacaaaatg tattagaaga
      301 gtatatattg ttgcgtgaaa ctatttataa tgatttaact gatatagaaa aagattatat
      361 agaaagtttt atggaaagac taaatgcaac aacagttttt gagggtaaaa agtgtttatg
      421 ccataatgat tttagttgta atcatctatt gttagatggc aataatagat taactggaat
      481 aattgatttt ggagattctg gaattataga tgaatattgt gattttatat acttacttga
      541 agatagtgaa gaagaaatag gaacaaattt tggagaagat atattaagaa tgtatggaaa
      601 tatagatatt gagaaagcaa aagaatatca agatatagtt gaagaatatt atcctattga
      661 aactattgtt tatggaatta aaaatattaa acaggaattt atcgaaaatg gtagaaaaga
      721 aatttataaa aggacttata aagattgatt atataatata tgaaaagcta ttataaaaga
      781 cattagtatt aaatagttta aaaaaatgaa aaataataaa ggaagtgagt caagtccaga
      841 ctcctgtgta aaatgctata caatgt
//
LOCUS       DAFBQH010000213          689 bp    DNA     linear   BCT 05-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate P-2341
            SAMN22135342-rid13226223.guided.203, whole genome shotgun sequence.
ACCESSION   DAFBQH010000213 DAFBQH010000000
VERSION     DAFBQH010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22135342
            Sequence Read Archive: SRR16347143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001162988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001162988.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/28/2021 00:26:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,960
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 24 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22135342-rid13226223.guided.203"
                     /isolate="P-2341"
                     /isolation_source="rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Boston"
                     /collection_date="2018-07"
     gene            1..650
                     /locus_tag="LK694_002960"
                     /pseudo
     CDS             1..650
                     /locus_tag="LK694_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          286 a           62 c          120 g          221 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccga atcattaaaa aaacattata
       61 cagagccttg ggaagatgaa gtttttagag taattattga atataacaat gttcctattg
      121 gatatggaca aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa
      181 ctgatgagat agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag
      241 gaattggtac aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta
      301 atgcagttat tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat
      361 ctggttttag aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag
      421 attgttattt aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat
      481 atttaattga gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg
      541 gttatgatag tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta
      601 ctaataagaa aaaaggttat gcaaaagaaa aagcaatata taattttttg ataaagtccg
      661 tataattgtg taaaagtaaa aaggccata
//
